LOCUS       DAJVRL010000001        84321 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVRL010000001 DAJVRL010000000
VERSION     DAJVRL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.001"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OQN02_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OQN02_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS1640960.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OQN02_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OQN02_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1640961.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OQN02_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OQN02_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OQN02_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OQN02_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCS1640962.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OQN02_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OQN02_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1640963.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OQN02_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OQN02_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1640964.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OQN02_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OQN02_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1640965.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OQN02_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OQN02_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1640966.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OQN02_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OQN02_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1640967.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OQN02_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OQN02_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1640968.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OQN02_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OQN02_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1640969.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OQN02_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OQN02_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1640970.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OQN02_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OQN02_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1640971.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OQN02_000014"
     CDS             18619..18894
                     /locus_tag="OQN02_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1640972.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OQN02_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OQN02_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1640973.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OQN02_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OQN02_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1640974.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20840..20911
                     /gene="ypdK"
                     /locus_tag="OQN02_000017"
     CDS             20840..20911
                     /gene="ypdK"
                     /locus_tag="OQN02_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1640975.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="OQN02_000018"
     CDS             complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="OQN02_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1640976.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22591..24077
                     /locus_tag="OQN02_000019"
                     /pseudo
     CDS             22591..24077
                     /locus_tag="OQN02_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24092..24826
                     /gene="ypdB"
                     /locus_tag="OQN02_000020"
     CDS             24092..24826
                     /gene="ypdB"
                     /locus_tag="OQN02_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1640977.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24839..25696
                     /locus_tag="OQN02_000021"
     CDS             24839..25696
                     /locus_tag="OQN02_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1640978.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25699..28194)
                     /gene="ptsP"
                     /locus_tag="OQN02_000022"
     CDS             complement(25699..28194)
                     /gene="ptsP"
                     /locus_tag="OQN02_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1640979.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28219..29256)
                     /gene="ypdE"
                     /locus_tag="OQN02_000023"
     CDS             complement(28219..29256)
                     /gene="ypdE"
                     /locus_tag="OQN02_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1640980.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29256..30339)
                     /gene="ypdF"
                     /locus_tag="OQN02_000024"
                     /pseudo
     CDS             complement(29256..30339)
                     /gene="ypdF"
                     /locus_tag="OQN02_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30354..31601)
                     /locus_tag="OQN02_000025"
     CDS             complement(30354..31601)
                     /locus_tag="OQN02_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1640981.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31623..31949)
                     /locus_tag="OQN02_000026"
     CDS             complement(31623..31949)
                     /locus_tag="OQN02_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1640982.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32168..33133)
                     /gene="glk"
                     /locus_tag="OQN02_000027"
     CDS             complement(32168..33133)
                     /gene="glk"
                     /locus_tag="OQN02_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1640983.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33337..34593
                     /locus_tag="OQN02_000028"
     CDS             33337..34593
                     /locus_tag="OQN02_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1640984.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34708..35034
                     /locus_tag="OQN02_000029"
     CDS             34708..35034
                     /locus_tag="OQN02_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1640985.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35175..36413)
                     /locus_tag="OQN02_000030"
     CDS             complement(35175..36413)
                     /locus_tag="OQN02_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1640986.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36749..37951
                     /gene="nupC"
                     /locus_tag="OQN02_000031"
     CDS             36749..37951
                     /gene="nupC"
                     /locus_tag="OQN02_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1640987.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38001..40113)
                     /locus_tag="OQN02_000032"
                     /pseudo
     CDS             complement(38001..40113)
                     /locus_tag="OQN02_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40321..40396)
                     /locus_tag="OQN02_000033"
     tRNA            complement(40321..40396)
                     /locus_tag="OQN02_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40361..40363),aa:Ala,seq:ggc)
     gene            complement(40436..40511)
                     /locus_tag="OQN02_000034"
     tRNA            complement(40436..40511)
                     /locus_tag="OQN02_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40476..40478),aa:Ala,seq:ggc)
     gene            40732..41091
                     /locus_tag="OQN02_000035"
     CDS             40732..41091
                     /locus_tag="OQN02_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1640988.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41093..41485
                     /locus_tag="OQN02_000036"
     CDS             41093..41485
                     /locus_tag="OQN02_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1640989.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41537..42952)
                     /gene="gltX"
                     /locus_tag="OQN02_000037"
     CDS             complement(41537..42952)
                     /gene="gltX"
                     /locus_tag="OQN02_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1640990.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43211..43286
                     /locus_tag="OQN02_000038"
     tRNA            43211..43286
                     /locus_tag="OQN02_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43244..43246,aa:Val,seq:tac)
     gene            43331..43406
                     /locus_tag="OQN02_000039"
     tRNA            43331..43406
                     /locus_tag="OQN02_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43364..43366,aa:Val,seq:tac)
     gene            43411..43486
                     /locus_tag="OQN02_000040"
     tRNA            43411..43486
                     /locus_tag="OQN02_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43444..43446,aa:Lys,seq:ttt)
     gene            43762..44103
                     /locus_tag="OQN02_000041"
     CDS             43762..44103
                     /locus_tag="OQN02_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1640991.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44094..45020)
                     /locus_tag="OQN02_000042"
     CDS             complement(44094..45020)
                     /locus_tag="OQN02_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1640992.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45110..46108
                     /locus_tag="OQN02_000043"
     CDS             45110..46108
                     /locus_tag="OQN02_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1640993.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46105..46323)
                     /locus_tag="OQN02_000044"
     CDS             complement(46105..46323)
                     /locus_tag="OQN02_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1640994.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46325..48340)
                     /gene="ligA"
                     /locus_tag="OQN02_000045"
     CDS             complement(46325..48340)
                     /gene="ligA"
                     /locus_tag="OQN02_000045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1640995.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48411..49397)
                     /gene="zipA"
                     /locus_tag="OQN02_000046"
     CDS             complement(48411..49397)
                     /gene="zipA"
                     /locus_tag="OQN02_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1640996.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49627..50388
                     /gene="cysZ"
                     /locus_tag="OQN02_000047"
     CDS             49627..50388
                     /gene="cysZ"
                     /locus_tag="OQN02_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1640997.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50573..51544
                     /gene="cysK"
                     /locus_tag="OQN02_000048"
     CDS             50573..51544
                     /gene="cysK"
                     /locus_tag="OQN02_000048"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1640998.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51928..52185
                     /gene="ptsH"
                     /locus_tag="OQN02_000049"
     CDS             51928..52185
                     /gene="ptsH"
                     /locus_tag="OQN02_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1640999.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52230..53957
                     /gene="ptsI"
                     /locus_tag="OQN02_000050"
     CDS             52230..53957
                     /gene="ptsI"
                     /locus_tag="OQN02_000050"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1641000.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53998..54507
                     /gene="crr"
                     /locus_tag="OQN02_000051"
     CDS             53998..54507
                     /gene="crr"
                     /locus_tag="OQN02_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1641001.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54549..55400)
                     /gene="pdxK"
                     /locus_tag="OQN02_000052"
                     /gene_synonym="thiJ"
     CDS             complement(54549..55400)
                     /gene="pdxK"
                     /locus_tag="OQN02_000052"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1641002.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55505..55879
                     /locus_tag="OQN02_000053"
     CDS             55505..55879
                     /locus_tag="OQN02_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1641003.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55912..56635
                     /locus_tag="OQN02_000054"
                     /pseudo
     CDS             55912..56635
                     /locus_tag="OQN02_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56839..57750)
                     /gene="cysM"
                     /locus_tag="OQN02_000055"
     CDS             complement(56839..57750)
                     /gene="cysM"
                     /locus_tag="OQN02_000055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1641004.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57884..58981)
                     /gene="cysA"
                     /locus_tag="OQN02_000056"
     CDS             complement(57884..58981)
                     /gene="cysA"
                     /locus_tag="OQN02_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1641005.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58971..59846)
                     /gene="cysW"
                     /locus_tag="OQN02_000057"
     CDS             complement(58971..59846)
                     /gene="cysW"
                     /locus_tag="OQN02_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1641006.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59846..60679)
                     /gene="cysT"
                     /locus_tag="OQN02_000058"
     CDS             complement(59846..60679)
                     /gene="cysT"
                     /locus_tag="OQN02_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1641007.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60679..61695)
                     /gene="cysP"
                     /locus_tag="OQN02_000059"
     CDS             complement(60679..61695)
                     /gene="cysP"
                     /locus_tag="OQN02_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1641008.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61999..62790)
                     /gene="ucpA"
                     /locus_tag="OQN02_000060"
     CDS             complement(61999..62790)
                     /gene="ucpA"
                     /locus_tag="OQN02_000060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1641009.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62977..63777)
                     /gene="murR"
                     /locus_tag="OQN02_000061"
     CDS             complement(62977..63777)
                     /gene="murR"
                     /locus_tag="OQN02_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1641010.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63941..64837
                     /gene="murQ"
                     /locus_tag="OQN02_000062"
     CDS             63941..64837
                     /gene="murQ"
                     /locus_tag="OQN02_000062"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1641011.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64841..66265
                     /gene="murP"
                     /locus_tag="OQN02_000063"
     CDS             64841..66265
                     /gene="murP"
                     /locus_tag="OQN02_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCS1641012.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66445..67344)
                     /gene="yfeX"
                     /locus_tag="OQN02_000064"
     CDS             complement(66445..67344)
                     /gene="yfeX"
                     /locus_tag="OQN02_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1641013.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67440..68015)
                     /locus_tag="OQN02_000065"
     CDS             complement(67440..68015)
                     /locus_tag="OQN02_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1641014.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68076..68525)
                     /locus_tag="OQN02_000066"
     CDS             complement(68076..68525)
                     /locus_tag="OQN02_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1641015.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68512..68937)
                     /locus_tag="OQN02_000067"
     CDS             complement(68512..68937)
                     /locus_tag="OQN02_000067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1641016.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69151..70020
                     /gene="amiA"
                     /locus_tag="OQN02_000068"
     CDS             69151..70020
                     /gene="amiA"
                     /locus_tag="OQN02_000068"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1641017.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70024..70923
                     /gene="hemF"
                     /locus_tag="OQN02_000069"
     CDS             70024..70923
                     /gene="hemF"
                     /locus_tag="OQN02_000069"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1641018.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70929..71981)
                     /gene="eutR"
                     /locus_tag="OQN02_000070"
                     /pseudo
     CDS             complement(70929..71981)
                     /gene="eutR"
                     /locus_tag="OQN02_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72027..72527)
                     /gene="eutK"
                     /locus_tag="OQN02_000071"
     CDS             complement(72027..72527)
                     /gene="eutK"
                     /locus_tag="OQN02_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1641019.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72540..73199)
                     /gene="eutL"
                     /locus_tag="OQN02_000072"
     CDS             complement(72540..73199)
                     /gene="eutL"
                     /locus_tag="OQN02_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1641020.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74381..75172
                     /locus_tag="OQN02_000073"
     CDS             74381..75172
                     /locus_tag="OQN02_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641021.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75892..76287
                     /locus_tag="OQN02_000074"
     CDS             75892..76287
                     /locus_tag="OQN02_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1641022.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76305..76568
                     /locus_tag="OQN02_000075"
     CDS             76305..76568
                     /locus_tag="OQN02_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641023.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76591..77133
                     /locus_tag="OQN02_000076"
     CDS             76591..77133
                     /locus_tag="OQN02_000076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641024.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77272..77559
                     /locus_tag="OQN02_000077"
     CDS             77272..77559
                     /locus_tag="OQN02_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641025.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77598..77804
                     /locus_tag="OQN02_000078"
     CDS             77598..77804
                     /locus_tag="OQN02_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641026.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78116..78601
                     /locus_tag="OQN02_000079"
     CDS             78116..78601
                     /locus_tag="OQN02_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1641027.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78582..78860
                     /locus_tag="OQN02_000080"
     CDS             78582..78860
                     /locus_tag="OQN02_000080"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641028.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78853..80313
                     /locus_tag="OQN02_000081"
     CDS             78853..80313
                     /locus_tag="OQN02_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1641029.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80347..81156
                     /gene="murI"
                     /locus_tag="OQN02_000082"
     CDS             80347..81156
                     /gene="murI"
                     /locus_tag="OQN02_000082"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1641030.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81275..81969)
                     /locus_tag="OQN02_000083"
     CDS             complement(join(81275..81723,81723..81969))
                     /locus_tag="OQN02_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1641031.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82488..83861)
                     /gene="eutA"
                     /locus_tag="OQN02_000084"
     CDS             complement(82488..83861)
                     /gene="eutA"
                     /locus_tag="OQN02_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS1641032.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83836..84315)
                     /gene="eutP"
                     /locus_tag="OQN02_000085"
     CDS             complement(83836..84315)
                     /gene="eutP"
                     /locus_tag="OQN02_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1641033.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        21899 a        21343 c        20003 g        21076 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatata gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aagtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cacataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacccgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac ctcatgattt ttatagcttt ttcttaagtt ttttccttcg ctgaatatct
    20581 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    20641 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    20701 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct tttaaggtcg
    20761 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    20821 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
    20881 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    20941 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    21001 ttgctgctga cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    21061 tgacgaatac ggtcgtggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    21121 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    21181 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    21241 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    21301 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    21361 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    21421 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
    21481 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    21541 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    21601 acatcgtagc atttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    21661 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    21721 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    21781 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    21841 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    21901 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    21961 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    22021 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    22081 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    22141 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    22201 cgagtgtcag ccataatttt ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    22261 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    22321 aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga aatttttcta
    22381 cgctaacaag aaaatcacca ccaattttat taacattata atttcaataa atttaccagc
    22441 tagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    22501 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    22561 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    22621 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    22681 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    22741 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    22801 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    22861 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    22921 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    22981 aaaatcccca aagcacagcg ctggcgctgg atatcgccaa caaaacgcta ccgctgtttc
    23041 gccatgtcaa tagcgagtca ttacgcaagg tctgcgaaat tatccgcgat gacattcacg
    23101 ccgatgcggt ggcgattact aataccgatc atgtgctggc ctatgttggc gtgggtgaac
    23161 ataactatca gaatggcgat gacttcatta gcccgactac ccgtcaggcg atgaattacg
    23221 gaaaaatcat cattaaaaac aatgatgaag cccaccgcac accagagatt cattccatgc
    23281 tggtgatccc attgtgggag aaaggggtcg tgaccggaac gctgaaaatt tactactgcc
    23341 acgcgcatca gatcacctcg tcattacagg aaatggcggt cggtctgtcg caaatcatct
    23401 ccacgcaact ggaggtttca cgcgccgagc agctacgtga aatggcaaat aaggcagagc
    23461 ttcgcgccct gcaaagcaaa attaatcccc attttctgtt taacgctctg aacgctattt
    23521 catcgtcaat ccgtctgaat ccggataccg ctcgccagtt gatctttaat ctgtcgcgtt
    23581 atctgcgcta taacattgaa ttaaaagacg atgagcaaat cgatatcaaa aaagagctgt
    23641 atcaaattaa agactatatt gccattgagc aggcccgctt tggcgacaag ctgacggtta
    23701 tctatgatat tgatgaagag gtgaattgct gcattcccag cctgctgatc cagccgttgg
    23761 tggagaacgc cattgtccac ggtattcagc cttgcaaagg taaaggcgtt gtcaccatca
    23821 gcgttgcaga gtgcggaaat cgggtacgca ttgcggtgcg agataccggg cacggcatcg
    23881 ttccaaaggt gattgagcgg gtcgaagcga atgaaatgcc gggcaataaa attggcctgc
    23941 tgaatgtcca tcatcgcgtg aagttattgt atggcgaggg gctgcatatc cgccgcctgg
    24001 agccggggac ggaaattgcg ttttacattc ctaaccaacg caccccagtc gcctcacagg
    24061 ctacgttatt gctttgagcc ggagtgatat tgtgaaagtc atcattgttg aagacgaatt
    24121 cctggcacaa caggaactga gctggctaat taaagagcac agccagatgg agattgtcgg
    24181 cacctttgac gacggtctgg acgtgttgaa gtttttgcag cataaccgcg tcgacgccat
    24241 ttttctggat atcaatattc cgtcgctgga tggcgtgttg ctggcgcaaa acatcagcca
    24301 gttcgcccat aaaccgttta ttgtgttcat caccgcgtgg aaagaacatg cggtagaagc
    24361 gtttgaactg gaggcgtttg actacattct caaaccgtat caggagtcac gtattaccgg
    24421 gatgctgcaa aaactggaag cggcctggca acaacagcag accagcagta cgcctgccgc
    24481 gacggtaacg cgtgagaatg acaccattaa tctggtgaaa gatgagcgaa taatcgtcac
    24541 gccaattaac gatatctatt acgccgaagc gcacgagaaa atgacctttg tctatacgcg
    24601 gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt tgcagcaaac tgccgccgtc
    24661 gcattttttc cgctgccatc gctcattttg tgtcaatctg aacaaaatac gcgaaatcga
    24721 accgtggttt aataacacct acattctgcg actgaaagat ctggattttg aagtgccggt
    24781 cagccgcagc aaagtgaaag aatttcgcca gttaatgcat ctttaatcgg gaactttcat
    24841 gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc gatctgttca gcggcctagt
    24901 gcttaacccg caactactgg ggcgcgtctg gtttgccagc cagcctgcct cgttaccggt
    24961 gggcagttta tgtattgatt ttccccgtct ggatatcgtg ctgcgcggcg aatacggcaa
    25021 cctgctggaa gcaaagcagc agcgaatggt ggaaggagaa atgctgttta ttccggcacg
    25081 cgcggctaat ttaccgatca acaacaaacc ggtgatgctg ttaagcctgg tgttcgcacc
    25141 gacctggctt gggttatcgt tttacgatag ccgcaccacg tcgttgttgc atcctgctcg
    25201 ccagacccag cttcccagcc tgcaacgcgg tgaaggtgaa gcgatgctta ccgccctcac
    25261 ccatcttagc cgttcgccgc tggagcagaa tatcattcag ccactggtgt taagtttgct
    25321 gcatctttgc cgcaacgtgg tgaatatgcc gccgggtaat tcacaaccgc gcggcgattt
    25381 tctctatcac agcatttgta actgggttca ggataattat gcccagccgc tcacccgcga
    25441 gagcgtggcg cagtttttta atatcacgcc caatcatctg tcaaaactgt ttgctcagca
    25501 tggaacgatg cgttttatcg agtatgtgcg ttgggtgcga atggcgaagg cgaggatgat
    25561 tttgcagaaa tatcatctgt caattcatga agtggcacag cgttgcggtt ttccggatag
    25621 cgactatttt tgtcgcgttt tccggcgtca gtttagtctg acgccgggag agtacagcgc
    25681 ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc agcagcgtca ggatactttg
    25741 tgcatcttgc gcggcaaaca acgactggcg gaagttttta ttcaccagtt tacgcgccag
    25801 ctgcgagaag actttcacat gattcatccc ttcgttagca cccagcgtca gcatgatcac
    25861 cagttcgact tcgcccattt ctgactgcca gtcaaccggt ttcgccagcc gggcaatgct
    25921 gatactggaa tgacggatcc actgagattt ggtgtgcggg atcgctacgc caaaaccaac
    25981 gccggtggta acaatctctt cccgctgcca gacatcttct tccagctcaa acggatgttc
    26041 agtgcgcccg ttaacgccga ggttgccgca caggaactgg atcgcctgct ctttattgct
    26101 aaaatcctga tcaacaaaga tattctccag cgccagcagt gggcgaacgt cttcttccgg
    26161 cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcactg cggcattcac atgcctgacg
    26221 cgccagttcc cgacacgcct cgctatccag ttgacgaagc tggcttttca ccgccggaat
    26281 acgcgggcta ctcatactca gctcatccag gcccagccca agcagtagcg gcagataacg
    26341 gctttcaccg cccagttcac cgcaaatgcc tacccatttg ccccgctgat gcgcagtggt
    26401 aactatttgc tgcaacatgc gcaggaacga tggcgtaatc gggttatata gcggcgatac
    26461 gcgcgggtta ttacgatcga ccgcatacag atactgggtc atatcgttgg agccgatact
    26521 gaagaaatcg acctcatcgc agaagtggtc gatgatgtag cacaccgacg gcacttccac
    26581 catgatccca agcgtaatcg tctctgcatg acgcaggcca tcgcgcttaa gctcaacgat
    26641 cgctttttgg atctcgcctt tcacccataa gatctgatcg aggctgtgaa ccatcgggat
    26701 catcaactgg gcgttgccga aactggcggc gcgcaaaatg gcccgcagtt gagtgcggaa
    26761 caggccagca aattccgggt aaatacgtac cgcgcgatag ccgaggaacg ggttctcttc
    26821 ctggggaata ttcagataag gaatgctttt atcgccgccg atgtccatcg tgcggaagat
    26881 aatcggcttg tcgcccgccg ccagtagcac ctgctggtag gcttcaaatt gctcctgctc
    26941 gtccggcgcg ctgtcgcgat ccatatagag catttcggta cggaacagcc cgacaccttc
    27001 cgcgccgttg gcaaacgcgc ctggcgcttc cagagcggtg ccgatattcg ccgcaatatc
    27061 aatacgcttg ttatcacggg aataggccag ctgcgcggca gcctgcgcct gttgtttttg
    27121 gcgtttatcc gccagcgtct gcgcgacctg ataataaccg ctcaccgcgt cattcgggtt
    27181 aatcgccagc acgccgcact gggcgtcaag cactgcaggt tgcccggcat aacgggcaat
    27241 cgcatccagc ggcaagccac tcagtaccgg gatcgccgag gcacgggcca gaatcagtgt
    27301 atgcgaggtg cgcccggttt tctccagaat catacccgca agatttttca aatcaaggct
    27361 caaaaactgg cttggggtta aatcttcagc caccagaatg gtcggttttt ccagcaccag
    27421 attgttgcgc ggcttcagtt ccggccaggt gatatgcagc aactgttcgc tgatatcgcg
    27481 aatgtcgctg acccgctcgc gcagataatc gctggcagaa gcagatagtt tggcgcaaac
    27541 ctgctccata ttgctgatga tcgccgcccc cagtccctga tgctgttctg tcatcaggcg
    27601 acggatattg cctgcaaatt catcatcctg aatcagcgac aaatgagcgc tgaggatagt
    27661 tttgctttcg ccgtcacgct cacgcaattg ctgattcagt tgctcggcaa gcgttgccag
    27721 gctgtgctcc agccgggtgg aatcttgcgc actggcgggg attgcccgat aactgtcgag
    27781 gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg ccgcttgcca gcacattgcc
    27841 gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc agcggatgcg ccgtcagttc
    27901 tgcctgcgtc ggctgaacgc tgtcgctgtc gataaagcgc accaggatgt actcttccag
    27961 cacgcgccgc gcctgctctt catcgctgcc gctaatgttc aggctgcaac tgtcattaaa
    28021 taaggtgcca gttccaatca acgccagcga gcttttagca tcagcctttg cgttctggcg
    28081 atggttgata aaggtgattt cgctttgcca ctggctgcac tgttctttaa gttcccacgc
    28141 cggacgagca tgtagaccgt taggcaaagg acagagaaat tgaatcgtta acataaccgc
    28201 tccgggttaa tcaggagatc atctgaaatc cgtcagttga acaaccgtct cacgcgtaag
    28261 acgttgaata agggcagata aaagttgctc catctgcaaa atgtcgcggc aatcggcaat
    28321 cgatgccgcg caatgtccgt ggcgggtggc aggccctatt accaatgtgg gtacgccagt
    28381 accggttaag tgcaccgctc caccgtccgt gccgccgtta ctgaacatat ccgcctgcaa
    28441 cggaacgcca atttctgccg ccacggtttc aatccacgcg gtaagttttg gcggcgcaat
    28501 cagcgattta tcgcttaaca ccagcatcgg cccgttacca atctgacgat ggttagccgc
    28561 gccataatca aagtttttcg cccagcaggc ggtatcaagc acaatggcga cgtccggcga
    28621 caccgcgcgg gtagcagttt gcccgccgcg taatcccacc tcttcgctgg aacttgccac
    28681 cagccacact tccgcaggca gttcggcgtc gtgcagctca cgtagtaacg tcaccagcag
    28741 atagcaactg aggcgatcat caaaggcttt gcccatcacc cgctggtgag ggaggacctg
    28801 aaaagtggta tcgaaggtga cgcgatcgcc tggacgaatt cccgcctgca tcacttcgtc
    28861 ataggtgcgc gcgccaatgt ccacgcgcat ggcgctaacg tcattcccct gccggtcgcc
    28921 gtcaagcagg cctggaattt tgcactcttc acgggtggtg atgcgcaccg gctgcagctg
    28981 gcgggcagcc atgcgcacgt tgccaaccgg cagcacgtca atagcgcctt cgcgggagat
    29041 gctgcgcacc ataaatccca cttcgtccat atgggcgcag atcatcacct tcggacctgt
    29101 cgattcattg aggcggatca gcaccgatcc cagaccatca aatcgcactt ctttttgcag
    29161 gcgagccgct tcttccagca ggatctgccg cacttcctgt tccgaggagg cgatcgcatc
    29221 tgcctcgctc aacgctttta atagcgataa atccattatg cctctcccgt gagcaacact
    29281 gttttcggca tggcgtagag cacttctgcg ccttgcgggg taaccagcac aacatcttcg
    29341 atgcgcacgc ccccttgccc tagcaaataa atccccggct ccacggtcag caacatgcct
    29401 ggctgtagcg tcgtggtgtc ccgcggtgaa aaacgcggat cttcatggac ttcaatgccg
    29461 atagcgtgac cagtgttatg accgaaatag tcgccaaatc cagcctcggt aatgacccgg
    29521 cgcgcggcgt cgtcaatctg ctggcagcgc acgccggggc gaattgcaga gattgccgcg
    29581 agctgtgcct gcaggacaat ctgatagacg tcaaacagcg ggtgagattc ggcgctcacc
    29641 ccttcgccat tcaccagcaa ggtgcgcgtc atatcagcag tagccctgat acagcgcacc
    29701 aaaatcgaga gtgacaaact cgcccgctgc aacaatcttg tcgctggctt tgccgtgcgg
    29761 cagcgccccg cgccagccgc tggcgacaat ggtgtcaaaa gaggcttttt ctgcgccctg
    29821 ctggcgcata aaccactcca gttcagcggc tatctcgcgc tcgctcatcc ccgcctgaat
    29881 aaagcggcga atatgctccg ctccgcgatc ggcaatccca caggcgaggc ggattatctc
    29941 cacctcctct ggcgttttga tttgccgcag cacatccggc gtggcgctaa ccagtttcgc
    30001 attgagttca gacttccagc ggtgcgcggt ttcccaactc acctgctggc cttcaaaacc
    30061 gagcgtttgc aactgctcat cggcaatgat ttgattgacg atagtggtaa gcgtgttcgt
    30121 cgcgtcaagc aaatgcagct ggtagccttg cgcacgggct tccacctcaa cgaaatagcg
    30181 cgaatctacc agaatgtgcg cactttcacg gctaataacc acatagcctg atccggtgga
    30241 gatccccaga tgcggctgtt tgttctgccg tgaggagaga agcaccgcat ccagttgttg
    30301 caccttaagc cagttgcgca gcgaagcgag taatgtcatc tggattttcc ttcttacagg
    30361 ctatcgatta acaatttgcc tttgcggaac atcatcagac gcaggaacac caccatcagc
    30421 gcagtaatga ttgcccccag cgcgatcccc gccatataaa cgcccaggtt agtgaccagc
    30481 ggccatgccc agatagcgga ttccgggaac cattgcactg cgcccagcca gacagctgcg
    30541 gttgagccga caatcgcgcc gaccatatat gacggaatag cggtgatggg gctttccagc
    30601 gcaaacggaa tcgcaccctc actaatcccc ataaaggcca ggaacatcgc ggtcttaccc
    30661 tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta aacgacggtc aataatggtc
    30721 gccagaccca gaccaatcgg cggaataacg atagcgatag aacgagcggt gaccggtaat
    30781 acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg ctttgttgat cgggccacca
    30841 aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca tcagcgcgcc cttctcacct
    30901 gcggcagtca gcacagtacg gataccgccg ttgatccagc caccaaaggg agtgatgacg
    30961 tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg gtagcaaaaa tgtggttttg
    31021 aacgctaaca ggaaatccgg taactggatt ttctggttca tccatttcac aaggtaaccg
    31081 gcaacgatag aaataatcag cgcaccaatg aaggtggacg gcaccggcga agaggtcgcc
    31141 cattgcatag tgctgggatc gaagttcagc agttgcgtcg gctgggtaga catcaacccg
    31201 ccgataaaac cagccgggaa cgccagtttg ccaccgatag agttcgccac aaatgcagca
    31261 aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg actgcgacag ccaggcaaat
    31321 ttaagtaagg agaggtcgaa accggagaat ttcccgctat taagagcatc catgatgccg
    31381 atctcagcag gaattttaag ccagctataa gcaatcagct ggctgaaagc gaggatcaca
    31441 ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg acatcacatg ctgcgggagt
    31501 tcaccccaga agctgctttt agaggcctgc ggattcttaa ctgccgctgc cgcaccggat
    31561 gcgccaggca caacggttgc actgcgtttt ttaatggcca taacaattat ccctgtcgaa
    31621 tattactgtt gttcagaggc aatcatctct tcgatttctt tgatgatgcc cgcagcgttt
    31681 ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg attcgaaacg ttcgttatct
    31741 tccggggtaa ctgccacgga atggatgatg atggtcgctt cggcgatatc ctgcgccgtc
    31801 aggcgattct ggatgccgtc cgcgccctgg gtttcaattt tcacttcata accggcttct
    31861 accgccgctt cttccagcac ctgagcggcc ataaaagtgt gagctaggcc cattgggcag
    31921 gcacataagg caatcagttt cttactcatc gttaattcct cgttcagtga tagttgacga
    31981 gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga caaataatgc atttactgga
    32041 gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc ttgtgagggg acacaaagaa
    32101 aaattgaatt aactgtatga tttaaaagat tatcgggaga gttacctccc gatataacag
    32161 gaaggattta cagaatgtga cctaaggtct ggcgtaaatg tgcaccggaa ccgagaagac
    32221 ccggatagtc atggacgatg agatacaccg gaatatcatg gacatattct ttaaagcgcc
    32281 ctttatcttc aaatgcggca cggaaaccgg aggctttgaa gaactcaagg aagcgcggca
    32341 cgataccgcc cgcaataaac acgccgccaa atgtcccgag attgagcgcc agattgccgc
    32401 caaaacggcc cataatgacg caaaacagcg acaatgcgcg gcggcaatcg gtgcagctgt
    32461 cagccagcgc gcgttcggta atatcttttg gcttgagatt ttctggcagg cggttgtcag
    32521 ctttcacaat tgcgcgatac aaattcacca gcccagggcc agaaagcacg cgctccgccg
    32581 aaacatgacc aatttccgca cgcaatattt cgaggataat ggcctcttct tcactattcg
    32641 gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac ccaacgctta tcgacatgga
    32701 ccagatgcgc aaccccaagc cccgttccgg caccgtaaac cgcaataggc ttaccttcga
    32761 ccggttctgc gccaccaaac tgaatcagat gctctttttt cagcatcggg atcgccatcg
    32821 atacagcggt aaaatcgtta ataatttcca gatggctaaa accgagattc tttttcattt
    32881 cggcaattga gaacgcccag gtatggttgg tcattgccac ccagtcaccg gtaattgggc
    32941 aagcgatggc aatacagccg tctttcacct cgaccttatg ttcttcaaga taaacgcgaa
    33001 tgaccgcttc gaggctgggg taatcaagcc ctgaataggt cttagcctgc gagatttcac
    33061 cactggcaat atcacacaga gcaagacgtg cgttggtgcc gcccacatca ccgactaatg
    33121 catactttgt cattcttcaa ctgctccgct aaagtcaaaa taattctttc tcacactgta
    33181 aatacctggg ggcataacaa caacgccgga aaggcaggct ccctgtaaat atcgatctgg
    33241 gtcacacaat tactttatcg tttcagcacc aattgcagcg atgccttttt gcaagctggg
    33301 caaactaagt atctgacccc gcataaggaa tagaacatgc tccatccgcg agccagaacc
    33361 atgttgttat tatcgctccc cgccgtggca attgggattg cgtccagtct tattctgatt
    33421 gtggtgatga aaatcgcctc ggtattacag aatttgctct ggcaacgact gccgggaact
    33481 ctggggatag cccaggattc acccctctgg atcatcggtg tattaacgct aacgggtatt
    33541 gcggtggggt tggttatccg tttcagccag ggtcatgccg gaccagaccc cgcctgtgaa
    33601 ccgctgatcg gcgcaccggt tccgccctct gcgctacctg gacttatcgt agcattaatt
    33661 ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc cgatcatgac cgtcaatatc
    33721 gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg tcaaccgaat ggagtggact
    33781 attttagcct ctgccggaac catcggcgcg ctgtttggta cacctgttgc ggcggcgttg
    33841 atattttcgc aaaccttaaa tggcagtagt gaagttccgc tatgggatcg tctttttgcg
    33901 ccgttaatgg cggcagcagc tggtgcactt actaccggat tatttttcca tccccatttt
    33961 tcactgccca ttgcgcatta cggacagatg gaaatgaccg atattctcag cggtgcaatt
    34021 gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat ggtgcttacc acggttgcac
    34081 gcgatgatga atcaaatgaa aaatccggtg ctcgtgctgg gtattggcgg atttattctc
    34141 ggaattctgg gggttattgg tggaccagtt tcgctgttta aagggctgga tgagatgcag
    34201 cagatggtgg caaatcaggc tttcagcacc agcgattact ttttgctggc ggtaattaaa
    34261 cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg gtgggcgaat cttcccggca
    34321 gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc acgttcccgc cgttccagcg
    34381 gcaataaccg tttcctgcgc tattctcggc atcgtgctgg tggtaacacg cgatggctgg
    34441 ttaagtcttt ttatggcggc agtcgttgta cccaatacca cattgctacc gctgctctgt
    34501 atcgtcatgc ttccggcatg gctgttatta gcaggtaagc cgatgatgat ggtcaatcgt
    34561 ccgaagcaac agccacccca cgataatgtt tagcaaaaaa tgctctcctt tatgatttaa
    34621 gagttatggc gatgatacgg ggctttacct ccccgtaata ttgcgttaac aggccgctga
    34681 cggcttcgat cgtgaaggag aataacaatg ttcaggtcac tgtttctggc ggccgccctg
    34741 atggcattta ccccgcttgc agcaaatgca ggtgaaatca ccctactgcc atcgatcaaa
    34801 ttacaaattg gcgatcgcga tcattacggt aattactggg acggtggtca ctggcgagat
    34861 cgtgattact ggcatcgcaa ttatgaatgg cgcaaaaacc gctggtggcg tcatgataat
    34921 ggctatcatc gtggctggga taagcgtaaa gcatatgagc gtggctatcg tgaaggctgg
    34981 cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg ggcaccgcca ttaaacgcgt
    35041 cctccaatgg agcacaatgc ctgatgcgac gctagcgcgt cttatcaggc ctacaaaccg
    35101 gctctttaac gcgtagggcg gataaggcgt ttacgccgca tccgccaata gtgccagatg
    35161 cgacgctcat tcaactacaa tcccagcgcc gtccccacca acaaccagat attcagcgcc
    35221 acgaccagca ctacaatcac ccagcctgtc tgttttacgc gtttgctgtt caccagatcg
    35281 cccatcaact tgctgtcact ggtgaaaatc agcagtggaa ccagcgccag ggcgatacca
    35341 aaacttaaca gcacctgact cataaccaga atccgtgtcg gatctaatcc catcagaatg
    35401 acaataaatg acggcaacat ggtgactgta cgacgcaccc acagcgggat atggaagcga
    35461 atgaatccct gcatcaccac ctgccccgcc agtgtcccca ccaccgttga ggacagtccg
    35521 gcagcaacca gacttaaccc aaagaccgtt gccgcagcat ggcttaacag cggttgcagc
    35581 gtcagataag cctcatcaag atcggcaaca ccagtatgac cagaaaagtg gaacgccgcc
    35641 gcagctgtag ccatcatcac cagattgaca aaaccggcaa tcgtcatggc gatagccaca
    35701 tcccatttgg tggcggaata acgttgttga cgcgaaccgc catgtaaatg ctgagtgagc
    35761 gaagagtgca aataaatcac atgcggcata atcgtcgccc ctaacacgcc tgctgccagg
    35821 aagaccgcct ccgaagtagg taaactcggg atcaccattc ctttacccag ctgcgccagg
    35881 ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa cagtaacccg
    35941 ccaatcactt tctccagcgg tttttgcccg cgacgttgca gcattaaaat caggaaagtc
    36001 gcgatccccg tcagcaccgc gccctgcaac aacgaaacac caagaatgag tttaaaaccg
    36061 atcgccgcac caataaattc cgccaggtcg gttgccatcg caataatttc tgcctgaacc
    36121 caatagaacc acactacggg acgcggatag tgatcacgaa tctgctccgc cagattttta
    36181 ccggtggcaa tccctagttt ggcagagagg atctgaatca gcatcgccat caggttggcc
    36241 caaacgacaa cccacagtag ctgatagccg aagctagcac ccgcctgaat attggtcgca
    36301 aagttaccgg gatcgatata accaatcgcc gcaatgaacg caggtcccat taatgcgagc
    36361 ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt catcttgtgc
    36421 ctctaaaaca tagcctttgc tatgtttcat gctatgctaa acgagaatga ttatcaaatt
    36481 catttaaatg gattgtggtg atttctctga tagaccaaga ttatgattac gaaaagattg
    36541 atgacttgaa tgttatggtt atgtttaatg ttagcacatt tacataactt tcagcttcca
    36601 tacacaacat agcagaaatg tatgacagat cactattttt gaagcctgtc acaggacgtc
    36661 attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg tgcacggaaa
    36721 tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg tactggcact
    36781 tgccgttgtt gtgattctcg cactgctggt aagcagcgac cgcaaaaaaa ttcgtatccg
    36841 ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc tgaactccga
    36901 cgttggttta ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc tcggatttgc
    36961 caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg cattcttctt
    37021 cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg atcggtattc tccagcacat
    37081 tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg ctctccaaag tcaacggtat
    37141 gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt cagaaaactt
    37201 tattgcctat aaagatatcc tcggcaaaat ctcccgtaat cgtatgtaca ccatggctgc
    37261 cacggcaatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca tgctggaacc
    37321 gaaatacgtc gttgctgcgc tggtactgaa catgttcagc acctttatcg tgctgtcgct
    37381 gatcaatcct taccgtgttg atgccagtga agaaaacatc cagatgtcca acctgcacga
    37441 aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca aagttgccat
    37501 tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga acgcactgtt
    37561 tgccaccgtt actggctggt ttggctacag catctccttc cagggcatcc tgggctacat
    37621 cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc aagtgggcag
    37681 tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc tgcagaaaat
    37741 tgcttccacg ctctctccgc gtgctgaagg catcatctct gtgttcctag tttccttcgc
    37801 taacttctct tcaatcggga ttatcgcagg tgcagttaaa ggcctgaatg aagagcaagg
    37861 taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg tgagtgtgct
    37921 gtctgcgtca atcgcagcac tggtgctgta aaaccataca taaaaaagcc ggggatcatt
    37981 ccccggcttt tttacgcctg ttaatcagct aatggctgcg ggcgaccaat caaataccct
    38041 tgcagatatt gcaccccgag cttatgcaat agcgcctgct gctgttgcgt ctcgacaaac
    38101 tccgcgacca cactcaatga cttcgctttc gccagatcgg taattgatct cacaatcatc
    38161 gcatccagcg tattcgtgac aatatctttc acaaagacgc catcaatttt gatgatatca
    38221 gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc atcaatcgca
    38281 atccggaaac caaacttatg cagctgctcg atgttgtaca tgctgctttc tgcgttagaa
    38341 aacgcctgct cctcggtgat ctcaagaatg accgcctgcg gggagatgtg ataacgctta
    38401 aacagacgaa taatccgccc ggcaatattc ttttgcagca gcgtgagcgg cattaaattg
    38461 actgaaaagc gcggtccttt tttgtcgcaa gggtgtgttg ccagccactt caacaaggat
    38521 tccagcactt gcaaatcaaa acgcgcgcta aggttaaact gagcaataag gggcagaaac
    38581 ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg cgaggatctc atcataacct
    38641 tcaccctctt tgttgcgaat tggctgggcg tagagcagca aatcaccctg atctaacgcc
    38701 gtgcgaatgg tattcagcaa tagcacctgt ttagtggtct gcccggaaac catctcctct
    38761 ctgctatcca gcgccagcac atgatgatgt gcgcaggatt gctccgccag ccagcttaac
    38821 tgccccaaca agggttgcag ggtttcctga tttccatcaa aacgccccca ggcagcacca
    38881 tagcccatat ccagcccggt attgttccag tgaatttgcc gactattcag gatgttaacc
    38941 atatgctgga gtcgcccttc cgtttccggc ccgctcagca ctaacagcag ttcactaccc
    39001 ggcaattgat acaacttttc gttttcctgc atcaacggca gcagcgtacg gcaaattgag
    39061 cggatacagt gaacgcgcat cattaagccg taatgacgac tcataaactc aagattatca
    39121 atgcgcaggc agcaaaaact cttgcccgcc tcttgctccg gcgcttgttc caacgcacga
    39181 aagttgggta ggagcgttaa cggatctgtt agcgcctgca aatgccagcg gcgattaagc
    39241 cattcactgc gatgataaat gcgcaccata tagagcaggc aaacgctaaa ggaaatcagc
    39301 accgcgagaa taaatgccag cgaatattcg gtttccaccc cttgcaaaaa gttctggttg
    39361 taattcagaa ggcaaagcgt tgaaacagcc caggtgagat ttaaaaacgg atagcgaaga
    39421 ttaccgaccc cgagggtaaa gatgatgaag aaaacgggta ccaggtaacc ggcaataaag
    39481 tcgttttcat aaggtgtgca caacagaagt agcagcacgc ttaacgctgc cagccagctt
    39541 aaggtaaatg cgagtgagat agtagaaaag catgttgtaa atcagcactg cggtgaaaag
    39601 gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg ccgaaaaagg tggatatctt
    39661 tagcgggaaa tcaaagaagc tccccacaag atacatgctg catttgatgc cgatcggcgt
    39721 caccaaacca agccagaaca gacgttgcca gacatatcgg ctggtcaatc cataacgcca
    39781 acgcgtcccc agctgccagc gtaatattgc gcaggcacac aacacgacaa acgtctggct
    39841 gccgagcatg accgcagtct gtaaaaaaga gagatgaaag ttccacagat tggtgcaaaa
    39901 catcccgcat aaaacgggaa cgacaccgcg ccagccaaag ataaacaaga ttgacagcat
    39961 tacgcacagt ggcatccacg ccagaaagat ataactggaa tggataatgg caagtggcga
    40021 aataaaacgg gatagctgaa taagtaccac ggtgagcgta aacgctagtg tgaatatctt
    40081 gatatttttt atcaggttat gctccacaaa catgagtaca ctactctcac tgatcggggt
    40141 tttcaggcac acaaataata agtttattcg catcgcgaag caaacagggg ccagcctgtt
    40201 gccgattttt catccaatga tgctttcacc aggcggcgct aacccggacg tagactgtat
    40261 tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt tgcagcgagg gttggaaatt
    40321 tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca
    40381 gctgagctat agccccacga tgcgtttacg taccaaattt gctgggtgca aaatttggtg
    40441 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    40501 gctatagccc cgtcacgtaa agcttgtcga gttgacgggc ggcatcatat gaattccgcc
    40561 cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc gctgaaaaat caggcaaatg
    40621 aacaattttg gaaaaccgct cgcattcagt agttattcat gtcacggttt cctgtaaagt
    40681 ggtgttataa aatgaactac taatagaccc acatacattc agggaattgt tatgttcaag
    40741 gagaggatga caccagatga acttgccaga ctgaccggct atagccgcca gaccattaat
    40801 aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac ctggcgtcca gggtggcaaa
    40861 gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata ttcgcaatgc tgaacgtcca
    40921 gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc cgcttgaagt attgctggtg
    40981 acactggcga aagagatgac gccagttgaa caaaaacagt ttacatcctt gcttctgcgg
    41041 gaagggatta tcggattgtt acaacgctta gggattcgcg atagcaaata atatgaaaag
    41101 attacgcaat aaaatgacca ccgaagaact ggctgaatgc ctcggtgtgg ctaaacaaac
    41161 tgttaaccgt tggaccagag aaaaaggctg gaaaacggaa aaatttcccg gcgtgaaagg
    41221 cggtcgtgcc agacttattc tggtcgatac gcaagtttgc gagtttattc agaacacgcc
    41281 agccttccat aacacaccaa tgttgatgga agctgaagag agaattgctg aatacgctcc
    41341 tggagcacgc gccccggcgt atcgacaaat catcaacgcg atagataata tgacagacat
    41401 tgagcaggaa aaagtcgcgc aatttttgtc acgtgaagga attcgcaatt tcctcgcccg
    41461 tctcgatatt gacgaatcag cataaataaa aaacggcagg atattatctc ctgccgttta
    41521 tctttttaca cgctaattac tgctgatttt cgcgttcagc aataaaatcc agcgctttgt
    41581 tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac ggtaacatcc agtgctggag
    41641 actgccccgc accggttacg gcgacacgca gcggcatacc aactttaccc atacccactt
    41701 ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt ttcagcggtc cagtcagtaa
    41761 tcgcggccag tttgtcacga accacttcca gcggctgacg cgctaccgga cgcagatgtt
    41821 ttttcgccgc atcggcatcg aactcagcaa aatcttcgta gaaataacgg cagctctgtg
    41881 ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt caccagatca gccagctgcg
    41941 ggccgttacg ggtatcgata ttttcctgct caatgtgcca ctgtaagtga gtagcaacat
    42001 actccggcgg cagcgcgtta atgtagtgat ggttcagcca cagcagcttg tcggtgttga
    42061 acgcactggc agatttgctg acggcattca aagtgaagta tttgatcatc tcttcacgag
    42121 tgaagatttc ctgatcgccg tgggaccagc ccagacgcac cagatagttc agcagtgctt
    42181 ctggcaaata accgtcatca cgatactgca ttacgctgac tgccccgtga cgtttggaca
    42241 gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc gtaaaccggc accggcgctt
    42301 tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt gatatggtct tcgccacgga
    42361 taacgtgggt gatttccata tcccagtcat cgacaaccac acagaagtta taggttgggg
    42421 aaccatcggt acggcggata ataagatcgt ccagttcctg gttgctgaac tcgatcggac
    42481 cacggatctg atcgtcaaaa acaacagaac cttcctgcgg gttagcaaaa cgtacaacac
    42541 acggttcatc atcagcatga tgctcatggc tgtggcggca gcgaccgtca taacgcggct
    42601 tctcaccttt cgccatttgc tcttcgcgca gcgcttccag gcgctcttta gagcaatagc
    42661 atttataagc agtgccctct tccagcatct gatcgatcac cgcgttgtag cgatcaaaac
    42721 gtttggtctg gtagtacgga ccttcatccc actccaggct cagccagttc atgccatcca
    42781 taatggcttc gatagcttcc ggcgtggaac gctcaagatc ggtgtcttca atacgcagca
    42841 cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta aagagcagta cgcgcgccgc
    42901 caacgtgcag atagcctgtt gggcttggcg cgaagcgagt tttgattttc atgaaatggc
    42961 cttacgttta gaaagatgcc gacaaccggc aaatcctgga aaaattgagt gggcgatatt
    43021 ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc taccttacgc attattgttt
    43081 tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat cttgcttaaa tttacgacga
    43141 acgaacaatt tctttagaaa atgcgttgac tcattttgaa ctctccctat aatgcgactc
    43201 cacacagcgg gggtgattag ctcagctggg agagcacctc ccttacaagg agggggtcgg
    43261 cggttcgatc ccgtcatcac ccaccaacta ctttatgtag tctccgccgt gtagcaagaa
    43321 attgagaagt gggtgattag ctcagctggg agagcacctc ccttacaagg agggggtcgg
    43381 cggttcgata ccgtcatcac ccaccacttc gggtcgttag ctcagttggt agagcagttg
    43441 acttttaatc aattggtcgc aggttcgaat cctgcacgac ccaccaatgt aaaaaagcgc
    43501 cctaaaggcg cttttttgct atctgcgata ctcaaagatt cgaacctgca gcaggtttga
    43561 gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg cccgaaaggc gagcgaagcg
    43621 agtcatcctg cacgacccac caatgcaaaa aagcgcccta aaggcgcttt tttgctatct
    43681 gcgatttgta agattgattt ttcttctcaa gttccttgtt cttctaccga tattacctcg
    43741 tacagtgacg aggactgaac aatggccact atctcttcaa cttcaattcc ttccatacaa
    43801 acacagagta gtaacagggc atcacaaggt agcgatgttg cttcacaaat cgcgcggata
    43861 agtcagcaaa tcatcaaact cactcaacaa attaaagaga ttgtagacac tagcggcagt
    43921 gctgaagaca agcaaaaaca ggccgaattg attcaacagc aaatcaccct actggaaaca
    43981 caactggcgc agttgcagaa acagcaggca gaaaaggcgc aggagaaaga gcagcgtttg
    44041 tctctgaatg tatcccttct aaatcctgtt gaaaaaacga ctcatatcga tatctatatc
    44101 tgatacaacg gatccccttc ccgaccagcc attagcgcat tgccctggtc tcgtaccacg
    44161 tcccataacg cttcagcggc ggtggaaagt gaacggttct tcctgcgcac cagcatcaac
    44221 tggcgttcaa ccaccggcgt aatgcgctta accaccagtg ggctaccttc aggcaatggt
    44281 agcgccagtg caggaagaat actgatgccg atgcctgcgg ctaccatcgg gaacagcgtc
    44341 gccggatgtc caatctcctg cacaatattc gcctgaatac cattgcgcgc cagcgctgcg
    44401 tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttcgc tccctgtaat
    44461 gcctgccagg gtacataatc ttccacggcc aaagcgctat cgcgatggca aagcagaaag
    44521 aaaggctcgg aaagaatcgc ttcacattgc agatcgccca ctgggcctgg atcgatgacg
    44581 atgccaaagt cgacatctcc ctggcgaata ctttccatca cccactgctg cggacgatca
    44641 tgcaggacaa actgaatatc tggatagcgg cgatggcttt cagcaatgca ttgcggaata
    44701 agatgcgcgg aaatggtctg gctggcagcg acccgaactt ttccgctcag ttgttgcccc
    44761 atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca gcagtcgctc aagacgcaaa
    44821 gccaactgct gccctgcatc tgtaagcacc acttcacgcg tggttctgtc cagcaggcga
    44881 acaccagtat gattttccag ctccttcaca ctgtgactta ctgccgactg gctcaagccg
    44941 atacgctctc ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa aacctttaat
    45001 tgttttaaag aataattcat ctgttttctt catgaatgga tgcaataaat caattttatt
    45061 tctcaaactg gaagaagcac aatagaacca tcgatcatct ggagtcttta tgaaactttt
    45121 tcgtatcctc gatcctttca ccttaaccct gatcacggtg gtgttgctgg cctctttctt
    45181 tccggccaga ggcgatttcg tccccttctt tgaaaatctg accaccgcag ctattgccct
    45241 gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg attattgctg gcggtggtca
    45301 ctggcgactg catttgtggg taatgtgcag caccttcgtg ctgtttccga ttctgggtgt
    45361 actgtttgcc tggtggaaac cggtaaatgt cgacccgatg ctctactccg gttttctcta
    45421 cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc ttcacgtcaa tggcgggcgg
    45481 taacgtcgcg gcggcggttt gttctgcgtc agcatccagc ctgctgggga ttttcctttc
    45541 accattgctg gttggtctgg tgatgaatgt tcacggtgca aggggcagcc ttgagcaggt
    45601 cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg gggcatcttt cccggccgtg
    45661 gattggtgac tgggtgtcgc gcaataaaaa atggattgcg aaaactgacc agacgtccat
    45721 tctgttggtg gtttatacag cgttcagcga agccgtcgtt aatggtatct ggcataaagt
    45781 tggctgggga tcattgctgt ttatcgtggt ggtcagctgc gttcttctgg ctatcgtgat
    45841 tgtagttaac gtctttatgg cacgccgact gagcttcaat aaggcagatg aaattactat
    45901 cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc ccgatggcaa acattctgtt
    45961 ccccacatcg gtgatcggta tgatggtgct gcccctgatg attttccatc agatccaatt
    46021 gatggtctgt gcggtgctgg cgcgtcgata caaacgccag accgaacagt tacaggcgca
    46081 gcaggaaagc agcgccgata aagcttaaag cggacgcttc aggggctgga ccagttgcgt
    46141 cagcccctcg gttttaatca gcagcgtgat ttgcattagc tcaccgagct tccctgccgg
    46201 gaactcatct ttgcgggcaa accacagcag atactcctcc ggcaggtcaa ttaagcgacg
    46261 ccctttgtat ttgccaaacg gcattatcgt attggctatt tcaatcagct gctctttttc
    46321 catctcagct acccagcaaa cgcagcattt ccgcttcgtc gatgacttca atgcccagtt
    46381 cctgcgcctt cgccagttta gatcctgcag cttcacccgc tatcaccaga tcggttttct
    46441 tcgacacgct gcccgcgact ttcgcgccca gttcgaccag tcgagcttta gcgtcatcac
    46501 gcgacatctg gcttaagctg cccgtaagca ccacggtttt accagcaaac gggctgtcaa
    46561 tctcttccgc gttgataacg atcggcgcag gccagtgaac accttccgcc aacagctcgc
    46621 tgatgacatt gcggttgctt tcttcggcaa agaagttgtg aacgtgggat gcaacgacaa
    46681 tgccaacatc aggcaccttt tgcagctctt caatcgaagc ggcttccagc gcttccagcg
    46741 tgccgaaata tgccgccaga cctgctgcgg tggcctcgcc gacttcacgg atgccaagtg
    46801 catagaggaa gcgagcaaag gtggtttctt tcgctttttc cagcgcgtta accacgtttt
    46861 gtgccaattt tggccccata cgctccagtc cggtcagttt gcctgcggtg agtttgaaca
    46921 gatctgccgg agtatggaca tattcttttt caaccagctg atcgatgatt ttgtcgccca
    46981 ttccgtcaac atccatcgca cggcgggaaa caaagtgttt cagcgactct ttacgctgcg
    47041 caccgcaaat caggccaccg gtacagcggg caaccgcttc accttccaca cgctcaacgt
    47101 cagaaccaca taccggacaa tgcgtcggga atacaacctc acgggtatct tccgggcgtt
    47161 cagaaagcac gacgttaacc acctgcggga tcacgtcgcc agcgcggcga atcaccactt
    47221 tatcgccaat gcgtaaacca agacgttcga tttcatccgc attgtgtaag gttgcgttac
    47281 tcaccagcac gccagcaaca tggacaggtt ccagacgcgc aacaggcgta atcgcgccag
    47341 tacgcccaac ctgaaactcg acgtcacgca caaaggtcat ctgctcctgc gccgggaatt
    47401 taaacgcaac cgcccagcgc ggggcacgcg cgacaaagcc aagctgctcc tgctgttcca
    47461 gtgagttgac cttaatcacc acgccgtcga tatcaaagcc cagcgtcggg cggtcttctt
    47521 ccactttgtg atagaacgcc agcacttctt ccgccgattc acaaagcgtt acccgatcgc
    47581 tgaccggcac cccccacttt ttaaattgca gtaaacggcc aagatgagta tccggcagct
    47641 cgccaccttc cagaacacca acgccatagc agaaaaaagt gagcggtcgc ttcgctgtaa
    47701 tacgcggatc aagctgacgc agtgaaccag ctgccgcatt acgtgggtta gcaaaaactt
    47761 tcccgcccgt gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc ggcaggaaca
    47821 cttcaccacg cacttccaga cgcgccggga tattctctcc gtgcagcttc agcggaatgg
    47881 cgcgaatagt acgcacatta gacgtgatat cttccccggt ggtgccatcg ccacgggtcg
    47941 cggcactgac taaaacgcca ttttcataca gaatactgac ggcaaggcca tccagcttca
    48001 gctcacagca ccaggtgact ttctcgttgt ttttcagacg gtcctgcaca cgtttgttga
    48061 aagcaagaaa gctttcttca tcaaaaacgt tatccagtga cagcattggt acttcatggc
    48121 gtatctggct gaaagctgcc agcggcgcag cacctacacg ttgggtaggc gaatcaggcg
    48181 taatcagttc tggatgtttg gtttccagct cgcgcagttc gcgcatcagc ctgtcgtatt
    48241 cagcgtcggg aatttccggc gcatccatca catgataaag atattcatga tggcaaagcg
    48301 tcgttcgcag ttctgtcagt tgttgttcga ttgattccat atcgcaccat caatgctaaa
    48361 aacccccgac aagcgggggt tcgaagagga gttaatttgc cttaagtgta tcaggcgttg
    48421 gcgtctttga cttcgcggat gatgtcctgg tactcgcgca atttctgcgg agtcatcata
    48481 cggcgctgat cgtcaagcac gacaccgccc acttcatcgg caatatgctg cgcagattgc
    48541 agcatcagct tgaagttctg cagctcgtca ccgtaagacg gtacctgcat aaagatagtg
    48601 acacccggag tagtgaaatc cttcatttca ggatcaaagg ttcccggttt caccatattc
    48661 gccaggctga ataacgccgg gccgctgcca tccgggctaa gatgacgatg gtaaatattc
    48721 atatcgccaa aaatgaagcc cgcttgttga atgctgttaa gaagcagttc accgtttagc
    48781 tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg cttctttgcg cttcggttta
    48841 tccataactg gagccggttc cgctacaggc tcaggctgtg gtgccgctac gggttctgca
    48901 ggctggaaag cctgttgtgc cggttgcggt gctgaatgca caggctgcgg cgctggcgcg
    48961 acctgtggcg aaactggctg ctgcaacggc tgttcaggct gcggctgata ggcaggctgc
    49021 tgcaccggct gcgctggacg cggagcatgt tgcggcggta cctgcgcttc aggcggctgc
    49081 tggaccggtt gacgcggctg cgcagacgca taaggcggta ggtactggtg ttgcggcgac
    49141 ggacgagcag cctcatgctc ctgagcgtta gccggggcat gattcacgcg gtgaacacga
    49201 acctcaccaa cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc gtcacgtttt
    49261 gacttcattc gttttaatgg ccgatcgcgg aacatagaag atcgttcttt acggctggtc
    49321 cagaaaccat gtaccagtaa agcgattatg gcgatcgcgc caacaatgat taatatcaga
    49381 cgcaaatcct gcatcattat attctctgtt gttctaacac cttgccacca cggcaaacat
    49441 ttactcacta agagtatttg ccgattacct caagtgcaag tgcactatta actttcacag
    49501 cacaaagata gatgaaatcg tgctttttgc tgttttttcg aacatatcct aactgtccat
    49561 tgcgcaatta cccggtaaaa tacgcagaat tttcctggga ttggtcaaaa ggagctcatc
    49621 ctgattatgg tttcatcatt cacatctgcc ccacgcagcg gtttttacta ttttgcgcaa
    49681 ggctggaagc tcgtctcgca acctgggatt cggcgtttcg ttattttacc gctgctggtc
    49741 aatattttgt tgatgggggg cgcattctgg tggctcttta cacagctcga tgtctggatc
    49801 ccgactttca tgagttacgt tccggactgg ctacaatggc tgagttatct gttgtggcct
    49861 ctggctgtca tctctgtgct gttagtgttt ggctatttct tctccacgat tgctaactgg
    49921 attgccgctc cgtttaacgg tttattggct gaacaactgg aagcacgatt gactggcgct
    49981 acaccgccag ataccgggat tttcggtatc atgaaagatg tgccgcgaat catgaaacgc
    50041 gaatggcaaa aatttgtctg gtatctgccg cgcgcaattg tattactaat tctttacttc
    50101 atccctggta ttgggcaaac cgtcgcgccg gtactgtggt tcctgtttag cgcctggatg
    50161 ttagccatcc agtattgcga ttaccccttc gataaccaca aagtgccgtt taaagagatg
    50221 cgcaccgccc tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt aaccagcctg
    50281 tttacgatga tcccgctgct taatctgttc atcatgcccg ttgccgtttg tggcgcgacg
    50341 gcgatgtggg tcgattgcta tcgcgataaa cacgcgatgt ggcggtaaca atctaccggt
    50401 tattttgtaa accgtttgtg tgaaacaggg gtggcttatg ccgcccctta ttccatcttg
    50461 catgtcatta tttcccttct gtatatagat atgctaaatc cttacttccg catattctct
    50521 gagcgggtat gctacctgtt gtatcccaat ttcatacagt taaggacagg ccatgagtaa
    50581 gatttttgaa gataactcgc tgactatcgg tcacacgccg ctggttcgcc tgaatcgcat
    50641 cggtaacgga cgcattctgg cgaaggtgga atctcgtaac cccagcttca gcgttaagtg
    50701 ccgtatcggt gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga aaccaggcgt
    50761 tgaactggtt gaaccgacca gcggtaatac cgggattgca ctggcctatg tagctgccgc
    50821 tcgcggttac aaactcaccc tgaccatgcc agaaaccatg agtattgaac gccgcaagct
    50881 gctgaaagcg ttaggtgcaa acctggtgct gacggaaggt gctaaaggca tgaaaggcgc
    50941 aatccaaaaa gcagaagaaa ttgtcgccag caatccagag aaatacctgc tgctgcaaca
    51001 attcagcaat ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg aaatctggga
    51061 agataccgac ggtcaggttg atgtatttat tgctggcgtt gggactggcg gtacgctgac
    51121 tggcgtcagc cgctacatta aaggcaccaa aggcaagacc gatcttatct ctgtcgccgt
    51181 tgagccaacc gattctccag ttatcgccca ggtgctggca ggtgaagaga ttaaacctgg
    51241 cccgcataaa attcagggta ttggcgctgg ttttatcccg gctaacctcg atctcaagct
    51301 ggtcgataaa gtcattggca tcaccaatga agaagccatt tctaccgccc gtcgtctaat
    51361 ggaagaagaa ggtattcttg caggtatctc ttctggagca gctgttgccg cagcgttgaa
    51421 actacaagaa gatgaaagct ttaccaacaa gaatattgtg gttattctac catcatcggg
    51481 tgagcgttat ttaagcaccg cattgtttgc cgatctcttc actgagaaag aattgcaaca
    51541 gtaatgccag cttgttaaaa atgcgtaaaa aagcaccttt ttaggtgctt ttttgtggcc
    51601 tgcttcaaac tttcgcccct cctggcattg attcagcctg tcggaactgg tatttaacca
    51661 gactaattat tttgatgcgc gaaattaatc gttacaggaa aagccaaagc tgaatcgatt
    51721 ttatgatttg gttcaattct tcctttagcg gcataatgct taatgacgta cgaaacgtca
    51781 gcggtcaaca cccgccagca atggactgta ttgcgctctt cgtgcgtcgc gtctgttaaa
    51841 aactggcgct aacaatacag gctaaagtcg aaccgccagg ctagacttta gttccacaac
    51901 actaaaccta taagttgggg aaatacaatg ttccagcaag aagttaccat taccgctccg
    51961 aacggtctgc acacccgccc tgctgcccag tttgtaaaag aagctaaggg cttcacttct
    52021 gaaattactg tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt taaactgcag
    52081 actctgggcc tgactcaagg taccgttgtg actatctccg cagaaggcga agacgagcag
    52141 aaagcggttg aacatctggt taaactgatg gcggaactcg agtaatttcc cgggttcttt
    52201 taaaaatcag tcacaagtaa ggtagggtta tgatttcagg cattttagca tccccgggta
    52261 tcgctttcgg taaagctctg cttctgaaag aagacgaaat tgtcattgac cggaaaaaaa
    52321 tttctgccga ccaggttgat caggaagttg aacgttttct gagcggtcgt gccaaggcat
    52381 cagcccagct ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa gaaaaagaag
    52441 ccatctttga agggcatatt atgctgctcg aagatgagga gctggagcag gaaatcatag
    52501 ccctgattaa agataaacac atgacagctg acgcagctgc tcatgaagtt atcgaaggtc
    52561 aggcttctgc cctggaagag ctggatgatg aatacctgaa agaacgtgcg gctgacgtac
    52621 gtgatatcgg taagcgcctg ctgcgcaaca tcctgggcct gaagattatc gacctgagcg
    52681 ccattcagga tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa accgcacagc
    52741 tgaacctgaa gaaggtgctg ggtttcatca ccgacgcggg tggccgtact tcccacacct
    52801 ctatcatggc gcgttctctg gaactgcctg ctatcgtggg taccggtagc gtcacctctc
    52861 aggtgaaaaa tgacgactat ctgattctgg atgccgtaaa taatcaggtt tacgtcaatc
    52921 caaccaacga agttattgat aaaatgcgcg ctgttcagga gcaagtggct tctgaaaaag
    52981 cagagcttgc taaactgaaa gatctgccag ctattacgct ggacggtcac caggtagaag
    53041 tatgcgctaa cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac ggcgctgaag
    53101 gcgttggtct gtatcgtact gagttcctgt tcatggaccg cgacgcgctg cccactgaag
    53161 aagaacagtt tgctgcttac aaagcagtgg ctgaagcgtg tggctcgcag gcggttatcg
    53221 ttcgtaccat ggacatcggc ggcgacaaag agctgccata catgaacttc ccgaaagaag
    53281 agaacccgtt cctcggctgg cgcgctatcc gtatcgcgat ggatcgtaaa gagatcctgc
    53341 gcgatcagct ccgcgctatc ctgcgtgcct cggctttcgg taaattgcgc attatgttcc
    53401 cgatgatcat ctctgttgaa gaagtgcgtg cactgcgcaa agagatcgaa atctacaaac
    53461 aggaactgcg cgacgaaggt aaagcgtttg acgagtcaat tgaaatcggc gtaatggtgg
    53521 aaacaccggc tgccgcaaca attgcacgtc atttagccaa agaagttgat ttctttagta
    53581 tcggcaccaa tgatttaacg cagtacactc tggcagttga ccgtggtaat gatatgattt
    53641 cacaccttta ccagccaatg tcaccgtccg tgctgaactt gatcaagcaa gttattgatg
    53701 cttctcatgc tgaaggcaaa tggactggca tgtgtggtga gcttgctggc gatgaacgtg
    53761 ctacacttct gttgctgggg atgggtctgg acgaattctc tatgagcgcc atttctatcc
    53821 cgcgcattaa gaagattatc cgtaacacga acttcgaaga tgcgaaggtg ttagcagagc
    53881 aggctcttgc tcaaccgaca acggacgagt taatgacgct ggttaacaag ttcattgaag
    53941 aaaaaacaat ctgctaatcc acgagatgcg gcccaattta ctgcttagga gaagatcatg
    54001 ggtttgttcg ataaactgaa atctctggtt tccgacgaca aaaaggatac cggaactatt
    54061 gagatcattg ctccgctctc tggcgagatc gtcaatatcg aagacgtgcc ggatgtcgtt
    54121 tttgcggaaa aaatcgttgg tgatggtatt gctatcaaac caacgggtaa caaaatggtc
    54181 gcgcctgtag acggtaccat tggtaaaatc tttgaaacca atcacgcgtt ctctatcgaa
    54241 tctgatagcg gcgttgaact gttcgtccac ttcggtatcg acaccgttga actgaaaggc
    54301 gaaggcttca agcgtattgc tgaagaaggt cagcgcgtga aagttggcga tactgtcatt
    54361 gaatttgatc tgccgctgct ggaagagaaa gccaagtcta ccctgactcc ggttgttatc
    54421 tccaacatgg acgaaatcaa agaactgatc aaactgtccg gtagcgtaac cgtgggtgaa
    54481 accccggtta tccgcatcaa gaagtaattc ttgccgcagt gaaaaatggc gcccatcggc
    54541 gccatttttt atgcttccgc cagcggcggc aaaatcaatt catcgctctc atgctgctgg
    54601 gtgtagcgca tcacttccag tacgcgcaac cccgcacggt gcaccgcatc gtttaacgcc
    54661 ttccctttca gcaaaccact gataagctga gcacaaaaca ggtcgccagt ccctttcagg
    54721 tcggttttta cccgtgaatg ggaaatgaca ttcacgctgt cggcactgac caccaccacc
    54781 tgcatctcct gattttcttc attaccggag gcgctggtaa tcaccaccca ttttaatgtg
    54841 tctgaaagca gactttttgc ggcagcaatg gcactgtcga gatcgcggca atttttaccg
    54901 gtcaggattt ccagctcaaa gatattgggg gtaattccct gcgccagcgg cagtaaatat
    54961 tgtcgatacg cctcgggaag gtcaggtttg acataaattc cgctatcaat atcgccaatc
    55021 accggatcga ccatgatcaa taggtcagga tggtctttgc gtagcgcagt cagccactcg
    55081 gcaaggattt tgatttgcga tgccgttccc atatagcccg tggttacagc acgaagttgg
    55141 cgcagcgcat cacgctcctg aagcgcacgc aaatagccgc taaaccattc gtccggaatc
    55201 gcaccaccgt agaaagtgtc ataatgcggc gtattgctca gcaataccgt cggcacggca
    55261 aagacattca agccgttctg ttttatagca ggcaccgcaa tgctgttgcc tacgcttccg
    55321 tacactacct gcgactgcac ggcgacgata tccgcctgca gcgccctact cttatcgtta
    55381 aacaacaaca aactactcat ttaatttttt ctccttgccg atgatcctca tcgtattcca
    55441 accgaaactt tacctgattc tggcagtcaa atcggctatt acaaagcaaa gataaggtaa
    55501 ttcagtgaag aaaatcattt gtctggccat tacgctgcta atgacactcc ccgcttacgc
    55561 gaagttaact gttcatgaag aagcccgcat caacgccatg ctggagggat tagcacagaa
    55621 aaaggatttg atatttgtgc gcaacggtga tgaacatacc tgcgatgaag cggtttccca
    55681 cctgcgtctg aagctcggca atacccgtaa ccgcattgac actgccgagc agtttattga
    55741 taaggtagct tcgtcgtcat cgattaccgg gaagccgtat atcgtgaaga tccccggtaa
    55801 gagcgatgag aacgcacagc cttttttaca tgcgttaatt gcgcagacgg ataaaacggt
    55861 gcctgcggaa ggaaattaat ccgctttggt aaggcattta caggaggtaa catgaaaaaa
    55921 tgctttattt atcacgatga aaaatcgaat aaattttggt ggatagatta cgaaggggat
    55981 agtttagctg tcaactatgg caaggtaggt agtattggta aattccatac aaaagaattc
    56041 gataatgaag aacagtgtct gaaagaagcc agtaaattga ttgccgcaaa aatgaaaaaa
    56101 ggatatcaag aaactccaga ctttaacttc atggatctct actattttga tgatgaagag
    56161 attgggttac atcttaaaac gtcacaccca aacttccagt gccattttac tgatccactt
    56221 tatatgtgtt gctgggatga agaatctcct tttggtagcg atgaaggtgc tgatgctcta
    56281 aacgaacttg aaaatagcct ccgttctgga ctgtgctgat ttccctcaaa tgttaattga
    56341 aactatgtgg ggtatgaaat acatcgctat ggacagtatt cttgaagagg atgtgcgtgc
    56401 gcaattacta gccgatgaaa tgagctctat ccagagcaat atgattacct acgcgactgc
    56461 attcggtcag atcaaagtca tgggtaaaat ctcccataaa cttaaaaaga tgggactcaa
    56521 tgcactagcg cgtcatcagc ttaccgcaaa aattcttcaa tggggtgacg gtcaggactc
    56581 accaatactt caaaaaatga ttgatgacct tactgctttt ccacacgaaa attaaaaact
    56641 gcatttgtcg gcagcaacaa ctgccgacaa atcagattat tgacaaagtg cactttgttt
    56701 atgccggatg cggcgtaaac gcctgatccg gcataaaaat attgctaatt caatatattg
    56761 cagaaacatt gtaggcctga taagcggagc gcatcaggca gttttgcgtt tgtcatcagt
    56821 ctccaatgct attaatcctt aaatccctgc cccctgacta aaatgctctt ccccaaacac
    56881 tccggtggag aggtagcgat cgccacgatc gcagatgatc gctaccacca ccgcaccagg
    56941 gttagctttt gccacccgca gtgctccagc aaccgcgccg cctgagctga cgccacagaa
    57001 tattccttcc cgcaccgcca gttcgcgcat ggtgttttcc gcatcgcgct gatgaatatc
    57061 cagcacctca tccaccagcg cagcgttgaa aatccccggc agatattccg caggccagcg
    57121 gcgaatgccc ggaatactgc tgccttcttc cggttgcagg ccaacaatgg tcaccggttt
    57181 ggattgttcg cgcataaagc atgaaacgcc ggtgatagtg ccggtcgtcc ccatgctgga
    57241 gacaaaatga gtgatgcgcc cgccggtttg ctgccagatt tccggcccgg tggtggtgta
    57301 atgcgcataa gggttatcgg gattattgaa ctgatcgagc agctttcctt cgccacgatt
    57361 cgccatctcc agcgccagat cgcgcgcacc ttccatgccc tgctctttgg tgacaagaat
    57421 cagttccgca ccataagcac gcatcgccgc acggcgttcc tggctcatgt tgtcgggcat
    57481 cagcaatttc atgcgatagc ctttcagcgc ggcaatcatt gccagcgcaa tgccggtgtt
    57541 accactggtg gcttcgatta agacatcacc cggtttaatt tccccgcgct tttccgcctc
    57601 gacgatcatc gaaagtgccg cacgatcttt caccgaacct gccgggttat tgccttccag
    57661 ttttaaccac acttcactgc cgttatccgg ccccattcgc tgcaacttca ccagaggcgt
    57721 attgcctatt gtttgttcta atgtactcac aatctctatc catacgtggt gttgcctgat
    57781 gcgacgcttg cgcgtcttat caggcctaca ggttacaaac cttgccatta aaaagcccgg
    57841 atcgcgggaa gcgcctccgg gcgtttaaca ttcactcaac ctatcaggcg ctttgtgcga
    57901 gagcaagttc ctcatcgcgg gtttcaatac gctcgtcgcc gttatacagc cgcgcatgtt
    57961 gcagaccaac gaataaacgc tcgccatgct gcggggcatc gtcgccatgc atcacgaccg
    58021 tcagcggttc gttgtaccac cccagcggct gcaccactaa ttgggtgtag tgacctttcg
    58081 ggctggcttc cagtacctgt accggcagcg gcgaatcgag actggtacgg cggctgatat
    58141 ccacttccca cgggcgcagg aagagatcca ccggcccctg atacgcaggt gtgtagccca
    58201 gcggccagcg atgcgcgcca acatggaact gcccgccgcg aatggttccc tgcaggcggt
    58261 tcacttcgcc cataaattcg agtacaaaac gggtcgctgg ttcgcgccat acctgattcg
    58321 gcgcatcagc ctgttcaata ttcccctgac tcatcaccac tacacgatcg gctacttcgg
    58381 tcgcctcttc ctggtcgtgg gtcacaaaca cgctggtgaa tttcagctct tcatgcagtt
    58441 gacgcagcca gcgacgcagc tctttacgca cctgcgcatc cagcgcgccg aacggttcat
    58501 caagcagcag aatttgcggt tctacagcca gcgcgcgcgc cagcgccaca cgctgtttct
    58561 ggccgccgga aagctgcgcc ggataacgat ccgccagatg cgcaagctgg accatttcca
    58621 gcaattttgt cactttcgct ttgatggctg cggcattcgg gcgctcgcga cgcggcagca
    58681 ccgtcaggcc aaaagtgata ttgtcgaata ccgtcatatg gcggaacagc gcgtaatgct
    58741 ggaacacgaa accgacttta cgatcgcgcg cgtgcaggcg actcacgtcg gtgccgtgga
    58801 agcgaatatg cccgctggtt tgatgctcca gcccggcgat aatccgcagc agcgtggttt
    58861 tcccggaacc ggacggcccc agcaacgcga ccatctgacc agaaggaata tccagtgaga
    58921 tatcgttcag cacctgggtg cgaccaaaag acttcttgat attggcaatc tcaatgctca
    58981 tgatgctcct cctgctgcgc gcgtttttcc tgattctcca ggcgccactg caacatactc
    59041 tttaaaaaca gggtgataat cgccatcaac gtcaacagcg cggcggcggt aaaggagccg
    59101 acggtgttgt agtcctgctc caacaattca atctgtaacg gcagcgaaag tgtttcgccg
    59161 cgaatcgagc cggaaaccac cgacactgca ccaaactcgc caatcgcgcg ggcgttggtc
    59221 agcaccacgc cgtaaagcag cgcccagcga atattcggca atgtgacgcg acggaacatc
    59281 tgccagccgg acgcgccaag caaaatcgcc gcttcgtctt cctggctgcc ctggcttaac
    59341 atcaccggca ccagctcgcg caccacaaac ggacacgtca caaagatggt gaccagcacc
    59401 attccgggcc aggagaacat aatttgcaga ttatgttcat ccagccagcc gccgagcggg
    59461 ccgttggagc cgtagaacag cagatacacc agaccggcaa ccaccggcga tacagcaaac
    59521 ggaatgtcca gtagcgtcag cagtaactgg cgtcccggga agttaaagcg cgtcaccagc
    59581 caggccagca gaatgccgaa caccaggttt accggtacgg caatcagcgc gatcatcacc
    59641 gtcagccaga tggcgtgcag catgtccgga tcggccagat tctgtaaaac cggcatcagc
    59701 cccttgctga atgcctgcac gaagatgtaa atcatcggca ccagcaggat gaacgccgaa
    59761 accagcatcc cgatgccaat cagaaaccat ttgccccagt taatcgggcg cgcgtcataa
    59821 cgcttcaatt gggtaacttc cgccattaat gacctaccac acgccgacca aagcgacttt
    59881 gcagagtgtt aattgagaac agcagcagca gagatgccgc gaggatcacc gaagcaatcg
    59941 cgctcgctgc cgggtaatca aactcctgta agcgcacaaa aatcatcagc gacgtcactt
    60001 ccgtcttcca cgcgatattt ccggcgataa aaatcaccgc gccaaattca ccaagactac
    60061 gggtaaacga cagcgccacg cccgccacca gcgccggaga aagctccggc agcaccactt
    60121 tgcagaaact ctgccagcgc gttgcaccaa gcgtttccgc cgcttcttca tattccgggc
    60181 ctaactcttc cagcaccggc tgcacggtac gcaccacaaa cggaatgctg gtaaaggcca
    60241 tcgccaccgc aataccaagc caggtatagg tgactttgat atcaaacttc gccagccatt
    60301 caccgtaaaa accgtttacg gaaaagagcg aggccagcgt taaaccggcg actgccgttg
    60361 gcagcgcaaa gggtaaatcc atcagcgcat caagcagcgt gcggcccgga aagcgataac
    60421 gtgttaaaat ccacgccatc agcagaccga aaacgccgtt aaaaatcgat gccacaaacg
    60481 ccgacagcag cgtcactttg taggccgcga ccacctgcgg gttggtgatc acctcccagt
    60541 actgcgccca gctcatctca gaaagctgca tcaccagcgc agagagcggc agcagcaata
    60601 tcaggcacac aaacagcaga ctggtgccaa ggcttaaagt aaagcccggc agcacgcgtc
    60661 ttgaagaaac tgcaaacatt acttacgccc cgccgctaac agcttgtcta actcgccgcc
    60721 gctggtgaag tgggttttca tcacttccgg ccatgagcca aatttgtctt ccacgcggaa
    60781 cagttcggtc tgcgggaatt tatctttcag tttgtccatg acttccgggt tattcacacg
    60841 gtagtaatag tcggtgatga tggtctgcgc ctgtgggctg tagagccagt tcaggtaggc
    60901 ttttgcggct ttttccgtac cgttggcctg cacattttta tcgacccacg ccaccgggaa
    60961 ttccgccaga atattggttt tcgggatcac cacttcaaag ccctgtgctt catactgttt
    61021 acggatgttg ttcacttccg attcaaagct aatcagcaca tcgcccaagc cacgctcggc
    61081 aaaagtggtg gttgcgccac gaccgccggt atcgaacact tcaacgtttt tcaggaactg
    61141 ggtcataaac tgttcggttt tggctttatc gccaccgtca gctttatccg ctgcgcccca
    61201 tgccgccaga taggtataac gcgcgttacc cgacgttttc gggttcggga aaatcagctt
    61261 cacgtcggag cgcaccaggt cgttccagtc gtggatattc ttcgggttgc ccttacgcac
    61321 caggaagccc atggtggagt agaacggcga gctattattc ggcaggcgcg actgccagtc
    61381 agccgggatc agcttgcctt tgtcgtgcag gatttgtacg tcggtcacct ggttataagt
    61441 gacaacgtct gcttttaagc cctgcaaaat cgccagcgcc tgttttgatg acccggcatg
    61501 agattgtttt atcgtcagtt tgtcgccgcc gttatctttt gcccattgct gttcaaacgg
    61561 cggattcagg gcggcaaaca gctcgcggga gacgtcataa gaactgttca gcagttccgt
    61621 tgcctgtaca tggcccgcca gcagcagaga agcgaccagc gcgagtgagt tctttttcag
    61681 taagttaacg gccattgcgc acccttataa atttaatgac tttctaatag ccatcatatt
    61741 tataacggac gtgaaaggag taacggtttt atataccgtt tggtgatttg gaagttaaaa
    61801 agggaataag aacttggttc aacaggcgta attgttgcag gcagtttgga cacggacagc
    61861 gcggagaaac cggagcgtac acatagtacg tgaggatttc gagcactgcc cggggccaaa
    61921 atgacaaatg aaatagcctg gtgaacttag ttcaagacga aatcctcccc acacatgcag
    61981 ggaggaaaca gaggtgaatc agataccgac gctaaccgtc tccggcagtg tgctgccgcc
    62041 atcaatcaca ttctgtgtac cggttaaata gctggattca tccgatgcga ggaaggccgc
    62101 cagttcgccg acttccagcg gatcggcgag gcgacacagc gggattgctt ttgccatttc
    62161 agtcagcacc gattctggat cttccgggtt cgactggcgg gcaatgcttt ccgccattgg
    62221 cgtgcggacg tatcccgggc agatggcgtt aacgcgaata ccggattgcg cgtactccac
    62281 cgccagcgat ttagtcaggc caacaatcgc cgctttcgtt aaggcatacg ccgtttcgcc
    62341 aggatcggcc accatatcac cagtgactga agacatcatc acaatgcgac catctttgcg
    62401 cgcaatcatc tccggcagca ccgccttcgt gacgttccat acgcctttaa tattgatatc
    62461 aatatggaaa tcgcgatctt catcgctcat atcgaggaaa ctgcccagac gacaaacgcc
    62521 tgcgttattc accaggatat caatgcgccc ttctttttcc ttcgcgcgtt tgatagctgc
    62581 ggctaccgac gccgggtcac gcacatcggc gacaaccgcc gtacagcgat gaccacgacc
    62641 acacagttcg tccgctagct tttcgatctc aggggagata tccagcaaga ttaggttcgc
    62701 gccatgacgt gcaaaagttc tggcaattcc ttcgccaatt ccctgcaatg cgcccgtaat
    62761 cagtgctgtc ttgcccgtga gtttacccat tttaatgctc tccttgttaa tacagagcgt
    62821 tacactccat tccctctgaa aatacaggat aaacaaaata ttgctgcgga tcagctcact
    62881 aattcaggaa aatctcaatc cagtcgctgc tttatgcatc atttcaggcg ttgtgttagc
    62941 tcactgctgc gctgaatcat tttcaatgac gccacatcat tgatgttgca caagaccaac
    63001 aaacagtaaa tcggtaacag aattttgcgc cgtgcgggta gacatcgacg agctacgcca
    63061 ttcggtttca ccggaaacgg tatcgagcgt gaaatgcgcc agccgccgca gcggagagtc
    63121 agccagagaa gtaatggcaa tcaccgttgc cccctgcttt cgtgccgctt ctgcgcacaa
    63181 gacaatctct tttttactgc cgctgtaaga gatagcgatt tgtacatcgc cttttttcaa
    63241 cgcctgggaa acggtggctt gtacgtgcgt gtcggcttcg caggccacgc gatagccaat
    63301 tttcatcagt ttgaacgaca gatcgcgccc caccagcgcc gatccgccca ggcccgttat
    63361 ctggataaac ggtgctttac tgatgacctc aatgattttt tgcagccgcg cgtagtcgaa
    63421 caacgcacag gtttgctcca gcgccagctc tttttcgcga ttcagtttgc gggcaatcac
    63481 ttccagcgaa tcatcactgg taatcgaact gtgcaggtgc agcgccgtgg cattcgtttt
    63541 ctcccggctg gcactgtatt cgccaattaa cgccatacgc aattcagtaa atccttgcgc
    63601 gccgagcttc tgggcaaatt tcacaatgct cgactggctg ataccgagct gtttcgccat
    63661 ctgccgtgaa gagacggatt tcagctcgct cactctggcc cgcagaaagt cagcaatttt
    63721 ttgctcgttc ccggtaaatt cactttccgc attgcgtatt ttcgtcaggt acaacatctt
    63781 ccactctcct gggctggcag caaaaattcc gcttaagttc ttatgacgct cttcacactc
    63841 tgcgagtgta gactcaatga ttcctttaag tctgtcaatt cgggatatat gattccatac
    63901 atctgcaatt atttgatcgt aaatagtaag gtcaccaccg atgcaactag aaaagatgat
    63961 tactgaaggc tcgaacaccg cctcggctga aattgaccgc gtatcgacgc tggaaatgtg
    64021 ccggattatc aacgatgaag ataaaaccgt accgcctgcc gttgatcgcg tcctgccgga
    64081 tatcgccgcg gcgatcgatg ttatccacgc ccaggtcagc ggcggcggtc gtctgattta
    64141 tctcggtgcg ggaacatccg gtcgtctggg gattctggat gccagcgaat gtccgcccac
    64201 ctacggcgtg aaaccgggtc tggtggttgg tttgattgct ggaggcgaat atgccattca
    64261 gcacgcggtg gaaggagctg aagatagccg ggaaggtggt gttaacgatc tgaaaaatat
    64321 taatttaacg gcacaggatg tggtggttgg cattgctgcc agcggtcgca cgccgtatgt
    64381 gattgccgga ctggaatacg cacgccagct cggctgccgc acagtgggaa tttcctgtaa
    64441 tccggggagc gccgtttcaa ccaccgctga gtttgccatt acaccgattg taggtgccga
    64501 agttgttacc ggttcttcgc ggatgaaagc aggtacagcg cagaaactgg tgctcaatat
    64561 gctttccacc gggctgatga ttaaatccgg caaagtgttc ggcaacctga tggtcgatgt
    64621 ggtcgccacc aacgaaaaac tgcatgtgcg ccaggtcaat atcgttaaaa acgccaccgg
    64681 atgtaatgcc gaacaagcgg aagcggcgtt aattgcctgc gagcgcaact gtaaaacggc
    64741 cattgtgatg gtgctgaaaa atctcgatgc cgcagaagct aaaaaacgcc tggatcaaca
    64801 cggcggcttt attcgtcagg ttttagacaa ggaataaccc atggccaaag agatcagcag
    64861 tgaacttctg aacaccattc ttacccgtgt cggcggaccg ggaaatatcg ccagttgtgg
    64921 taactgtatg acgcgcctgc gtctgggtgt acatgacagt tcactggttg atcccaatat
    64981 caaaacgctg gaaggcgtga agggcgtcat tttgaccagc gaccaggtgc aggtcgtttt
    65041 tgggcctggt aaagcgcatc gtgccgcaaa agcgatgagc gagctgctgg gcgatgcacc
    65101 ggtacaggat gccgcagaaa tcgccgccca gaacaaacgt cagttaaaag ccaaacaaac
    65161 ctccggcgtg caacaatttc tcgccaaatt cgccaccatc ttcacgccgc tgatccccgg
    65221 ttttattgcc gccggtctgc tgctggggat agcgacgtta attgccacgg tgatgcacgt
    65281 tccggcagat gctcagggca cgctacccga cgcgctgaat tttatgaagg tgttcagcaa
    65341 aggtttgttc accttcctag tgattctggt gggctataac gccgcccagg catttggcgg
    65401 cacgggcgtc aatggcgcaa ttattgccgc actctttttg ctcggttata accccgctgc
    65461 aaccaccggt tactacgccg gttttcacga tttctttggt ctgcccatcg atccgcgcgg
    65521 caatattatc ggcgtgctga ttgccgcgtg ggcctgcgcc cgcattgaag gcatggtgcg
    65581 ccgctttatg ccggacgatc tcgacatgct gctgacctcg ttaatcaccc tgctgatcac
    65641 cgctacgctt gcgtacctga tcattatgcc gctgggtggc tggttattcg aaggtatgtc
    65701 atggctgttt atgcacctga acagtaatcc gttcggttgt gcggttttag ccgggctgtt
    65761 cctgatcgcc gtggtatttg gcgtgcatca gggctttatt cctgtttacc tcgcgttaat
    65821 ggacagccag ggattcaaca gcttatttcc catcctttca atggcaggcg cgggccaggt
    65881 gggcgcggcg ctggcactct actggcgggc gcaaccgcac agtgcgctac gcagtcaggt
    65941 acgcggggcg attattcccg gcctgctggg cgttggtgaa ccgctgattt acggtgtcac
    66001 cctgccccgc atgaagccgt ttgttaccgc ctgtttaggc ggcgcggcgg gcggtttgtt
    66061 tatcggattg atagcctggt ggggtctgcc gatgggctta aacagcgcct ttgggccgtc
    66121 tggtctggtg gcgctgccgc tgatgacttc cgcacaaggc atccttccgg caatggcggt
    66181 ttatgctggc gggattctgg tggcatgggt ttgcgggttt attttcacca cgctctttgg
    66241 ctgccgtaac gtcaatctgg actgatatta tgaaacggac aatgctctat ctttctctgc
    66301 tggctgttag ttgtagcgtc agcgccgcaa aataccctgt tctgacagaa ggtagttgat
    66361 caagtatatg cggcgttaaa gcagtaataa aaaggccagt cagtaatgtt actgcctggc
    66421 ctttaatcaa tgaatcagaa acgcttacag cgccatcaac ttgtccagcg acggtgcgaa
    66481 ataatagccg ccggttaccg gtttggtgaa acgcaacatc gcatcacgtt tgccatcggt
    66541 atcgccaaac atgctcagca gttgctgctc aatgttatgc agacgcgcgc agtaggcgca
    66601 gaagtacaga ccgtgagtgc cactggcggt gccgtacggc aggctctggc gaacaatctt
    66661 cagccctttg ccatcttctt tcagatcaac gcgggtgagg tgagaggttt ccggacgttc
    66721 gtcgccgtca atctcttcgt tggcctcttt ggtgcgcccg atcatcatct cctgatcgtg
    66781 aacgctcatc cggttgagct gcttcaggtt gtgctcccaa cgctgcacaa acacatagct
    66841 gccgccggaa tccacgccgt ctttgataac cgccacttcg cggcgcgtct cttcacccgc
    66901 cgggttttct gtaccgtcaa caaagccgct caggtcacgc tcttccaccc aacggaagcc
    66961 gtggatctct tctttcactt caatgcagtc accaaaggct tccatcgccg cctgagcgac
    67021 agagaagttt acgtcgtgac gcagagaaag aatgtggatc aacacatcga actgggtcgt
    67081 cggcgcaagg cctttaccgt agcccggaaa atctttcagc tcttctgccc caacgccgcc
    67141 gctcagagcg cgccaggtgt tgttaccaaa ggcaaccacc gcaccaagat gcgcgtccgg
    67201 gaatttcgct tcaaaagttg ccagtttgtc ggcaaatgtt ttactggccg cacgcagggc
    67261 gtcaacttcc cctttcacgt tggcttcgat ccaaatcgcc gcgcggcaat gttctggcaa
    67321 aatgccactc tgaacctgag acattgttcc tcctgaaaat aagaatgcca cgcaatccgt
    67381 ggcattgttg agcgctattt tacgtgtttt ttcgcgacgg cggtttgttc agacgcaaat
    67441 taacgccgcc agataatttt actgactttc cagtttttca gggtatcgtc cgaaggcatt
    67501 aacccttcag gaccacgcca ttccccgctg aactggtagc taatatgttg actgccttcg
    67561 gctttacatt cgacggcacg attatcatca ccatcagctt tttggcaatt gccaaatgct
    67621 ttgctgtaaa ggtcgctaaa cggtgtaccg attttaacac cggtgtcagc aggaatatcg
    67681 ctatccagca cgtcaatgcg actgatcgtg ccttgatcgc cgttaatcac catcgccacg
    67741 ttgtcgcctt tcatcacttc aaaaaagcgc accacgttgc cgttcgcggt tttcattccg
    67801 ctgcgcaggc gataatcgcc atcaagcgca tcagcaatgg cttgttcttg cagtggtgtg
    67861 gacgccgtta attcacccac gccctgctcg ctcactttgg tggacgatcc aaaccagttc
    67921 cacgggttag cggcagacca gttaactgac gacatcgtgg aacagccggt cagcatcaac
    67981 ggcatagcgc ataacattaa acgcagcgat ttcatgtcac ttcctttggt tattcaataa
    68041 cgttgcttgg agtgcaaatt caccaaaaag tgccgttatt ctttttcttc attaaaacaa
    68101 gcgcgtaaac gtcgattggt cagcagccag attaacgcca caatatccgc cactaccagc
    68161 gccagaccga taccgctaac ggattcaccg ttcagccaca gccacggttg ccagcaaagt
    68221 aaaaccacct gcgccaacag caacagaaaa tagagcacat gccaggtgcg aggaaacgaa
    68281 gcccgccgac cgctcaacaa aaacgccagc accgccggaa taccgggaat cagccccagc
    68341 cagaaattat cgtgatcggg ataaaacaga tttagcagcg cagtaccctg ctcacgcgac
    68401 gcaccggcaa tgacaaacag cacccaggtt cgcgcctgaa gcaatagcac aagccagaag
    68461 agcaagggta aacgcaggcg tccgtgcgca tcataatgga caggatggaa ctcagtactc
    68521 ttcatcttca atcaaacgct tacccagact cagcacgtcg gcgtgttcat atcccaggcg
    68581 ttcatacatt ccgagcacca tgtcgttatc ttccggcacg ttgatctgaa ttttcgggca
    68641 gccgcgagca atcagctttt tctccagccg gttaagcaac gcattggcaa ttccacgccc
    68701 acgaaactct ggatgcacgc caagataata agcagacccg cgatgcccgt cataaccgcc
    68761 catcaccgtt ccgaccactt caccgtttac ctcagcgacc aaaaacaaac tgacgtcatg
    68821 gttcatctta cgctcgatgt ccatttccgg atcgttccac ggacgcagca agtcgcaacg
    68881 ctcccaaagg gtgatgacct cttcgaaatc ttcctggcga aatacgcgta tctccatggt
    68941 attcgttacc tttttgcggg ttaaaaggct gattatggcg tgaacggtcg aattagccaa
    69001 tatctgacga aaatcggttg aaaaagtggc ataatgggga gttgtcaact attgaaatga
    69061 aaagtaaaac aattctcaac agcaaaccgt cgtaacggat tacgcgatac gatataacat
    69121 ctggaacttt attattacaa ctcaggccgt atgagcactt ttaaaccact aaaaacactc
    69181 acttcgcgcc gccaggtgct gaaagccggt ttagctgccc tgacgttgtc aggaatgtcg
    69241 caagccattg ccaaagacga acctttaaaa accagcaacg gacacagcaa gccgaaagcc
    69301 aaaaaatctg gcggcaaacg tgtcgttgtt ctcgatccag gccacggcgg aattgatacc
    69361 ggagcgatcg gacgcaacgg ttcgaaagaa aaacatgtgg tgctggcgat tgctaaaaac
    69421 gtccgttcca ttttgcgtaa tcatgggatt gatgcgcgtt taacgcgttc tggcgatacg
    69481 tttatcccac tttacgatcg cgttgaaatc gcccataaac atggcgcaga tctgtttatg
    69541 tcaattcatg ccgatggctt taccaacccg aaagctgccg gtgcttcggt atttgccctc
    69601 tctaaccgtg gggcaagtag cgcaatggcg aaatacctgt ctgaacgcga aaaccgcgcc
    69661 gatgaagttg ccggtaaaaa ggcgactgac aaggatcacc tattgcagca agtactgttt
    69721 gatctggtgc aaaccgatac tattaaaaac agcctgacgc ttggctcgca tattctgaaa
    69781 aagattaagc cggtgcataa actgcacagc cgcaacaccg aacaagccgc gtttgtggtg
    69841 ttgaaatcgc cgtcggttcc ttcggtgctg gtggaaacct cgtttatcac caacccggaa
    69901 gaagagcggc tgttaggcac ggcggcgttt cgtcagaaaa tcgccacagc gattgctgaa
    69961 ggcgtgatca gttatttcca ctggttcgac aaccagaaag cacattcgaa aaagcgataa
    70021 gttatgaaac ccgacgcaca ccaggttaaa cagtttctgc tcaaccttca ggatacgatt
    70081 tgtcagcagc tgaccgccgt cgatggcgca gaatttgtcg aagatagttg gcagcgcgaa
    70141 gctggcggcg gcgggcgcag tcgggtgttg cgtaatggtg gtgttttcga acaggcgggc
    70201 gtcaactttt ctcacgtcca cggtgaggcg atgcctgctt ccgccaccgc tcatcgcccg
    70261 gaacttgccg ggcgcagttt cgaggcgatg ggcgtttcac tggtagtgca tccgcataac
    70321 ccgtatgttc ccaccagcca cgcgaatgtg cgttttttta ttgccgaaaa accgggtgca
    70381 gagcccgttt ggtggtttgg cggcggcttc gatttaaccc ctttctatgg ttttgaagaa
    70441 gacgccattc actggcaccg caccgcccgt gacctgtgcc agccatttgg tgaagacgtt
    70501 tatccccgtt acaaaaagtg gtgcgacgat tacttctacc tcaaacatcg caacgaacag
    70561 cgcggcattg gcgggctgtt ctttgatgat ctgaacacgc cagatttcga ccactgtttt
    70621 gcctttatgc aggcggtagg caaaggctac accgacgctt atttaccaat tgtagagcga
    70681 cgtaaagcga tggcctacgg cgagcgcgag cgcaattttc agctctaccg tcgcggtcgt
    70741 tatgtcgagt tcaatctggt ctgggatcgc ggcacgctgt ttggcctgca aactggcggg
    70801 cgcaccgagt cgatcctgat gtcaatgccg ccactggtac gctgggagta tgattatcag
    70861 ccaaaagatg gcagcccaga agcggcgtta agtgagttta ttaaggtcag ggattgggtg
    70921 taactccctc acccccattc ccgcatccgc tgatgcaacg tcaacgactg cttctcggca
    70981 aacagctgct ggtaatccgt ggcaaattgc cccagatgcc agaatcccca ctgcatggcg
    71041 gcgtctttta ccgttgtgct ttgcgaccac gggcttatca gttcgcggcg tacggcgttc
    71101 aggcgaatgc gtttcagcca cgcgtttggg ccaatgccta aaatagcgtg aaacgcgttt
    71161 tgtagcgtgc ggcgactgac atgcagttga ttacacaaat ccagcaccgt caccggttcg
    71221 gacatgtttt ccagcacata ttcacgggcg cgggaaagca accgacggta actctgatga
    71281 ctgatgcttt ccgccgtcac cattggttgc gcttcttcca gcatcgcccc catcgccatt
    71341 agcaaattat cccccagcac ttttcgcact gcaggctgat ggagattttc cgggttctcg
    71401 caaaacgtcg ccagcgcctg ttggacaaag ccccacagcg cggctttatg ctgctctttc
    71461 acttccagcg ccgactggct acgcagcata tgtaataccc gatccggatt gtgcaaaaag
    71521 ttagcctgac aggtgatgac atcttcagac agcactacgc ccaggatcgt ataatcgtcc
    71581 ggcgtactta attcaaactc ggttccaccg gggcgcgtcg ctatttctgc gcttcccaga
    71641 cattgcgaac cgataaatcc ctgctcaccg cgcgtcgccg gaatgccaaa ccagaacgag
    71701 ttcggccaga ccaggcacga ctgacgcagc gccagaccgg tgtattcacg aaaaacctga
    71761 atatcatcga gtagaatttc cgtaaattca ccatgaaact tgcccggatg cagctgatcg
    71821 taaatctgct gctaggcggt aatcgtcaaa gcatgttcat agacatccgt tgtctgtcgt
    71881 tgatgaacat tatccacttc gaccttcggc gtgagcttca ggttttcggg taagggttca
    71941 tgataaagat ggtgcaaatt ggctgtacgg gtctttttca tgatgttaat gccgggtgtt
    72001 gtaggacacc cggcacctcc gacaggttaa tggggcttga gacgataacg actactgcgt
    72061 ttacgtaacg tcccggcaga aaagagctgt tcgagcgcat ttctggcttt ttccagcggc
    72121 cagccaaagt gggcggccac ttctcctgcc gtcattccct gacatactga tgttaacagc
    72181 gccaacaact cgtcagcaga ttccgcgacg ataactggcg cgtcgggttc ctttacgggt
    72241 tgcttcggct ggcggttaaa gccagtaacc agccactggg tgtcatcgtc tggccgaccg
    72301 atctccttac ggctgatgac acgcccggta cgcatcgcgg cagcacaacc tgcgtccagc
    72361 gccgcacgac acgccgccag atcgccttcc accaccagcg ttaagcgacc agggtcaagc
    72421 acttcgtgac tgagcagacg aacgttagct gctttgagca tggcatccgc tgcatctatc
    72481 gcggcgacca tgccgtccac ttccagcaat cccagtgcat tgatcatcgg caacctccgt
    72541 tacgcacgct ggattggatt acgcgcgatt tccagcactg catcggtaaa ggcgttacag
    72601 gctgctttac acgcggcctg gctaccggtt aaaaatgctg ccgagtagtt ggtttcagac
    72661 ggtggcggga cgtaggtcac cagctgtacg tcggcagatt tcaacgcggc atcaatgccg
    72721 taggtcgctt ccagcggcgg tgccaccaga tacgccatcg gatcgccaag cgtgatcccg
    72781 gcggttgatg aaagataaga accggtacgc gaaaccacat gcgccaggaa ggcggtgttt
    72841 tctgcgtcat tggcccactg gaaagctgcg ccattttcaa tatgtgcaac catcgcatcc
    72901 agaccggcac gcacttccgc cgggtttggc ccaccaagca taatcagcac ttcaccggca
    72961 gtcggtgacg ggccgtgtgc cgctcccgca tacagcgagc gaccatatac cacttcgacc
    73021 atcgcttgtt tggtcgcttc gtcagccgca atgtaggtta cgtcgtcaga atcggcagaa
    73081 atcagcccga ggctacgaat atgcggcggc aattttagtt cacgcgcaaa ttcagcgtta
    73141 acggaggcaa tcacccgcat ggcggttacc gacggtcgaa tcaaatctaa agctggcatg
    73201 atgcctcctt atcgggtcat gttgatgccg gatgctttct gctccagcat acgtttcgcc
    73261 aaatccacaa tgacggcaca gcagcacgaa agagaagcaa aagtcagcta cctggtacac
    73321 taggcagcgg gtcagcaaag gggggcaaat gccccttttt tcatgtcaga taatcagatc
    73381 attacctcaa taaactaata atcacatcgc ggttacaacc agtttgatag tgatagctca
    73441 aacccagacg tggcgctggc tgcaagcaaa ataagccgca aaaatgcgac cccatgttta
    73501 ctagccgcaa aaatgcggtc ctatttgcca gaacctttta ttattcaatt ggttagtgca
    73561 agaaaaagcg tgattactgt tcaagaagta tccagaaagg tcatgatttt gacgctgaaa
    73621 aaaaataggt gggcattttt gcccactcac tagccgcaaa tttgcccact cactagccgc
    73681 atttttgcgg cccaaagtaa taaatacaga gaaataataa atacagatca gcgccaagat
    73741 ttttcgtctt ttcggcggaa tcatgagact agtgcttacg tcctgataac ctccagagca
    73801 gaccttaact tctgtgcagc aggactacgc tcattgccgc tcggctaggt caaatcctca
    73861 cgataaagct gttcggattg tgaccataat gttaaaggac tctttaaatc attccttgac
    73921 gaattaacta gctggcttaa gcggaatagc atgcaggata aaaacaaaac cagcttagca
    73981 gtggggagag cttcgcttcg ctcgtctcct ggggccaatg ctcaatgaaa cgacgtaagg
    74041 cacggagcgc tcaccatagg tagacacata aacatctgag agcgcacctt ctatcgcgct
    74101 acgcgctcaa taaaaaccaa cgacaccgcc acactgcaag accaatggtt tttatcgcgt
    74161 atacagacca gacaggaaac agcagcaaag cagcgtatag ccaggtgcag acagcacact
    74221 cacacgacgc acagcaggct taagtgatca ctcaggaaac tttatccccg ccgccgatca
    74281 ccgcgaacta tcatgaccgc gccaaattcg aaaggaatgg cccatagaac gcgatgaaca
    74341 cacataagcg ctacacttac ttaacgtcgc cacagagcga ttgagagcgc ttaaaatcga
    74401 tgcgatttca ttaaattttt ctcactcgcc tgaatggtgg cgatcaggac tgatcgcaac
    74461 attgccccgc ctcgatattg agcgcataat agatacatac attaagcgga aaagagataa
    74521 gtttattgtg aaaatcaggg ggttacatat catgcgcata tcagaacaac aacggcagat
    74581 cctcgactat ctggcaaaaa acgaatcaat caccgcacga caagccgcag atctcgttta
    74641 tggtggcaat gttacccgcg tccaggttga cgccgcccgc cgctcactca tgacgatggt
    74701 tagtaatggg ctaatcagga aacaaggccg cgctttcgta gctgaaagac atatcaatca
    74761 cgcttatagc caggacgttg aagaattgcg ccgccagatc ctttgggtat tcagcaaaca
    74821 gggtcaggag tatttccttt ctgaacaccc aaatttcgcc gccaaagagt taacagtaag
    74881 cggattgtcg tgcgcactgg attatcttgt cggtgaaggt tgcgttactt acaagcagct
    74941 atggcacgcc ctgaatacgc ttgtagaaga tggtttactc attgccacgc gtgagaacat
    75001 cgaatactgg ataatgccgc gttcaggtcg ccgcaatcat gcacggtata tttcaggcaa
    75061 cagatacaga acgccggact ttaatccgac acaggaagat cgggaccgtg ccgaaatgat
    75121 gtgcagggaa caggagaaaa gaattatgga taagttattt tcgatgcagt gaacacaagc
    75181 gggcgcaatg cccgttttta ttgccttatt ccacgcctga cgaatcaatt tgatcacacc
    75241 tgagaaaatg catagccaga agttaaggcc gttatattgc gattgggagc gttttaaacg
    75301 gtatcaattt aattaattct gatgtggtga tttgcaggca aaaaaaaccc gccggagaag
    75361 cgggaaatag agacatatcg ttgtagttat taacagatca ctttgaggtc gagggcggct
    75421 aaccctcgct aatattatcc cgctattacc ttgcgggtgc aaataatctt ttattgcttt
    75481 gatgatttaa taaatagcac tttttgctaa caaaaactgc tgtcaatata tggccaaaaa
    75541 tgtcaacaga aaaaataagg tttataatat taagtatcac atgggtaatg ggttcaggga
    75601 atcggaatcc accgcctcac ggaaattcaa aatttgcttc ttctttcctc cgtgggcggc
    75661 ttctttttcg tatcgaggta tttatgaaag caataacatt agaacagttc gcagcaatgg
    75721 cgaatgtatc gaccagaacg ctgaggcgct ggctaaaaaa accatcaacc ggagtccctt
    75781 tgccatttac cccacgcggt actcgccaat tattctggga gcacgaatgc aaagaatggt
    75841 tgtacagaac agaacgataa taatcacgca tcacattttg aggtaaacat catgaccaat
    75901 atcgaaagat tagaacgctg tgccgatatt atgcggcgca gatggattta tgaccctgaa
    75961 gcgggtttat tgtttagccg tgaaacaaaa gacgttatca aaggcagcat gaccaataaa
    76021 gggcatttgg ttgtaacggt acatgaaggt aaattcaaag tgacactcac ctatcagaaa
    76081 gcctgctatg tatacacata cggcgcttat gacgagacat tatatgaagt gcaccacgtt
    76141 aatttgaaca aacaggataa ccgcatcaag aatattcgcc tgatgccgaa agaaaagcac
    76201 cgccggatac ataaaaacgt ccgcaaactt attcggctgg gccacacaca atggcttcaa
    76261 ccgcaattaa ttgaacaatc actataaaca caggagtaaa taccatgcat tcattagaca
    76321 gttattttca gcgcacgaca gcccctaaat cagccgccca ggaacgccgt gaggaattcc
    76381 atgagaaagt gatgcgttct gctgattata ttgcggacaa gttcgtggaa acggttcgtc
    76441 cacttgttga tgaagtagct gataaacttc agtctgaaat gccggaagat atggaaggca
    76501 ctgcaaagcg gcgactcatt tgcgaattaa gtcgtcgttt tggcgtgtca ataagcgctt
    76561 ttaaataatc gaaaaaaggg gggaaagata atggacgcat ttgaggtact ggcaaataaa
    76621 ggcgctgaac tggtagcaca gcgtgataaa accgccaacg aaggggaacg cgcactgcta
    76681 aataaacaga ttaaagcgat ccgtatggcc cagtttaaat taatcagtaa tgaagttatc
    76741 gaaacaatta aaccgcagat cccacaaatc gttgctgacg ctattaaagc cgccggactg
    76801 gagaaacgca ttgccgggct aaaaacgaac gcagagaaaa tggagatgca caaacagagc
    76861 ggtgctccga atcccgatga gtacttctca ccggatgttg agtttatggc gcaggttgag
    76921 gaacgtctgg gtagctgtct caccgaggaa cagcgtcgct attttgatgg agtggatagt
    76981 tcggcaggta ttgacctgaa ctcgtatttc ggtcgtgaaa ttgagcattt tgatgccgcc
    77041 ccgtctatgc cggagccaga accagaaatg gccaccgatc ctgaagctga gcaacggaaa
    77101 cgcgtattca atgcgatagg gattcggtac tgatcggata aaagaaagcc gggaattacc
    77161 ccggctgact taacaatgtg accaatgaac aaatcggaca agacaaacag gataagcagt
    77221 ctcgaaacaa tgttcgggaa ctatattagc acaagtccgg agaaaatcat catgttggca
    77281 ctacgcatca tgcagggcat cgctaagacc ctcgcagaac atgtcttaga cctgaaacat
    77341 tcgccactga gcaaacaggc catgaagcgc caaacgcttc gtctgtgggc tgaatattcg
    77401 cttggaacta tcaataaaat catcgacatg aaatcggggc cgagcaacca gagcgcggag
    77461 gaaatggagt tcatcaggcg tttaattctc attcgcagag acattcatag ccagttacac
    77521 agcgtgggaa tcgatattaa tgatgggacg ggcgattaat tttcggacaa gtactatgcg
    77581 cgcgcgagga gctgaacatg aagcaagata ttgcagacag gctggagata ctcgaaggcc
    77641 agcgggcaga agcgaaacaa ctacgtaagc aggccagaag agcgcacagg aacaacgaag
    77701 cagagttact aaccaaatac atcagtttca ccaattactg catctacgag tgttacaagg
    77761 aagatgcaga ggactggctg gattcattgc cagaacagta ctgagcagaa taacaccacg
    77821 tcatgttgtt ctgaggtgag catatcacct ctgttcaggt ggccaaattt agtgtgccac
    77881 ttcagcatca ccgttgaaat cacaataacg atttttttgt aaatatgctc tcacatatgt
    77941 aggaatagac caggttggca ttgaaacaca tctaccatga ccataataaa gtaatttttt
    78001 gatatgttta tggttttata ttaattaaaa tatatctaaa attgatagtt ttaaaattgc
    78061 aaaatccaga ttgtgtttgt aatgtttaat ctgattgtat tgagtggcgt ttattatgaa
    78121 aaataaatca ggtaattctt gttatagaga agcagtcgat attcatagct cacttgagct
    78181 ttcatttata ttgcataaat atcatttgcg tgtagatgag aagaaatctc gaaatgtatt
    78241 cagcgccata cgtgatgagc tcatccttga tggggattca cgacaaaact tagctacttt
    78301 ctgtcaaaca tggcttgatg atgaaatccg tgagttgctt aatttagcta ttgataaaaa
    78361 catgatttat aaagatgaat atccacaaat ggcggaacct gaaggtcgct gtgtgcgtat
    78421 gctttccgat tcgtggagtt tccccgattc gcgacacacc ttaggttgct caaccattgg
    78481 ttcatctgaa gctgctatgc tcggccgctt ggcgttaaaa tggcagtggt gtaaaaaacg
    78541 cgaagtacaa ggtaaatcaa ccgaacctga tttgcgatcc tgtgcaaata tgctggcata
    78601 aattctgccg ttactgtgat gtgaaattgt gtgaaattcc acttgatgat catcacctga
    78661 taatgaatgc taaagaggtt ttgaagcatg tcgatgaaaa cactattggt gtcgttacga
    78721 ctttaggtat tacttatttt tgtaaagcag attgcgttag tgtttttaag ataagtgtta
    78781 aagttaatat tagtttttat atttacatcg taattatatg tttctatttt aaaaatgttc
    78841 gagagtataa aaatgtctaa tgctaatatc gcacccaaaa gtaagaaaat gacagtgggg
    78901 acactcgcta tcatgaatat aactgctgta gtaagtctac gatttatacc ttctgaagca
    78961 gaatacggct taggtgccat tttttactat acctttgctg caattatgtt tttagtacct
    79021 gttgcctttg ttgcggctga gctagctaca acatatccac aaaaaggggg ggcatttcga
    79081 tggataggag aagcatttgg ggctcgctgg ggttttgtag gaatgcttat gacctggcta
    79141 caagtcatcc cctactttcc aacagtactg acatttggtg cagtctcggt tgcatttatt
    79201 gatgttgata tgggggtggc agaatcaatt gccagtaata aatggtatat tttatttttc
    79261 gtactatttg tttactgggg agctatttgt gttgcattac gtggcgttgg tattttctcg
    79321 caggtatcta agtggtgcgg tattataggg acaattattc cagctattat ccttgttatt
    79381 cttggtttct cctatctgtg gttcagcggt aaaccagcgg tgatcccatt aggttggggt
    79441 gatcttttcc ctaatatagc taacttccag aatattgttc ttgccgccag catatttctt
    79501 gcttatgcag ggatggaaat gaatgcggtc catgttaacg atttagataa tccaactaaa
    79561 aaatacccaa ttgcaattac catcgcttct ataggtacta ttgcaatatt cctgctcagt
    79621 actttagggg tcgcctttat tattcctaca gataaaatta gtctaactca aagtttgttg
    79681 gttgcctacg atatgctgtt cgaatgggct ggagtgccat ggcttggctc tgtgatggca
    79741 tttatgctag caattggcgt actgggtggg gtggttactt ggattgcagg gcctaataca
    79801 ggtgtattag cgatcgcgaa agctggttat ttacctaagt ttttccaaaa aaccaatcgt
    79861 catggaatgg ggcaccatct aatgtttgta caaggtataa ttgtctcagt tctttctgtt
    79921 acttttgtga taatgccttc agttcaagca gcttttcaga ttttatcaca gttaacagtt
    79981 atgctctatt tagttatgta tatgctcatg ttcgctagtg ctatctatct acgacattct
    80041 caacctgata caaaacgccc atatcgagcc cctgctttat ttttctgggc aacacttgga
    80101 ttcttagggt cttttctggc ttttgtatta agttttattc cgcccgagca aatcccaact
    80161 ggtagtccaa ccagctatgt tctttatttg ttagtattag ttgccctatt tttattaatt
    80221 ccaatattaa tttacgcctt ccgaaaatct gactggaaag atccaaactc tgatttcgaa
    80281 ccattttctt gggaaaaaag aactagtaat taaattttcc ataagctaca aaagatgagg
    80341 ttaaatatgt ttagtgctga atctccaatt ggtgtaatag actcaggtgt aggtggattg
    80401 acatcggtaa aggaaataat taatttgctt cctggtgaag atattatcta ttgtggagat
    80461 aatatgaatg caccttatgg caaccgttct gccgatgata tcatcagttt gactaaaaaa
    80521 atgctgactt ttttacagtc aaggaatgtc aaactagttg ctgtcgcatg taatactata
    80581 tcctcaactt tggaatccga agaatacagc ggatatgcta agagctttcc gtttcctatc
    80641 ttatccatca ttgaacctgc agtagaagat gtgattcgcc agcaatataa aaatgttggt
    80701 attatcgcta ctgagtttac tattaaaacg gggtgtcaca aagagctaat aaaaaaattg
    80761 aattcaacaa taaatgtatt tggagaacca agcaaaaatc ttgcaatgtt aattgaggaa
    80821 ggtaatctta atgctcctgc aatcttgaat gacatcaaaa aacatgttaa ccatttaatt
    80881 tccctccatc cagtaaatga aattattctc ggctgtactc actatccaat tgttcagaat
    80941 ttttttgaag cagttgcacc ggatataaaa tttattaatc ctgcacatga tcaggctatc
    81001 tctattaaaa atcacttggg acagctaaat ttattaaact cctcaaatat aggaactttg
    81061 caaataaata ctagtggaag tatggagatc tacaaaacgg tactagggga attgagcatt
    81121 acgaaacctc atacattcag tatcaggcag ttttaaattt atccggtgca ccgtaaaatc
    81181 ccgtccttct gaactgcacc ccaaaagttg gacaccaact gagtaaggtg cattttatgg
    81241 acaaacccag ttacatactg ggtagtgcat ccaattagta gaacatgtgt ttttcgataa
    81301 acgctccgat cactttttcg tggatctcaa ctgagcgtga gaagcagatt gttttacgac
    81361 ccaaccgctt aatgcgggtg cgtagcgtca gattattacg ctcaatgcgt tgggtgaata
    81421 ttttgccggt cagatgctta ttcttcggca cctcccggcc atagctgccc cagtcatcgc
    81481 tggtcagcat gccgatgttg aagggtgtaa gcagtgccag tagctcccgg cacgtttgat
    81541 cggttcgggg accaaaagtg taggccagta caccgcctgt tttggtgttg tacgcgtacc
    81601 cgagccaatg ttgccgggct ttactgccaa cgtagctcca ttgctcatca agctcgcaga
    81661 taaacgccac atcagcatgg gcaacaggcg aagaagttat tcgctttggc gcgagttttt
    81721 taaagtccgg atgacggtgt tactaccaat tttcagtgtc ctggcggtat cgcgaacccc
    81781 ggcaccatta aaggccattt cagttatcag ctctttcata cccggcttac gtgcttgata
    81841 agtataagtg aactgaaaca cacggtggca gtcacggcag cgaagtctgt cacggccttt
    81901 agggttctga ccatggcggt aaacctgagc tgactgacaa cggggacaat gaatattaac
    81961 gctggtcatg agataacctc aaaagcccgt attatacatc agattcaact aattagaggc
    82021 atcacccatc cagcattata aaacatacta acaataacta aattataata atcacaactt
    82081 acaatcaaat aataaatgca taagattaac aatgattttt tcgggttata tttatctaaa
    82141 aaagcagaat attatgtaac ttattatttt ctaagcgaaa atttattgtg cggtcagact
    82201 aacaaacttg cgtaccaccc gaagaacatg cgaagattta agtattcttt cttttttggt
    82261 ttttattttt cattttctct tttcttctct ttccggttct gttctggttt tggttttttc
    82321 ctttttattt ttattctttc ttcagggtta tgctaaaaca ccaaaacatt tttcaccaca
    82381 aaagcatcca atcatcttgc cagaccaatc accttaaccg gatacactgc cggatataat
    82441 cccactaaca cccagacacc aagcaccata cctgataact accgtattca ggaaggaaat
    82501 gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt accaatgtcg
    82561 atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag ttgttgtagc
    82621 tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac caccagcagg
    82681 ggatgcggat tcggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac cgtcagaacc
    82741 gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacacgtc agttttggga
    82801 tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt ttcatcaatc
    82861 gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctccagcca gattgtgctg
    82921 ccagagagcg aaagggtatg tgcacccgcg ccaatcaccg tggagcgtac ggtttgcgcc
    82981 ggaaactgca cattcatctc acgcaggcgc ggatggtcat gcagcgccgt tgccagaagt
    83041 gggccaatat cggcaaaaca gaacgggttg gcgggctggt ggcgataaca ttcgcccacg
    83101 cctccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc
    83161 aatgcctgcg cgagcggcga aagcgttccg tcaatcactt cgacaatcag cgccgccatc
    83221 cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcagt gcctgcaccg
    83281 aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg cccctggctg
    83341 tcggtttcca gcaggcgacc accgacgttg agacaggcag tgccgctgat ttttccggca
    83401 tcgaacaggg cgtagttcgc ggtgccaccg ccgatatcga tattcagtac ccgacacagc
    83461 cgttgttcag aaagggtttg cgccccggct ccgtgaccgg cgatcacgga ttcgaggtgc
    83521 ggcccggcgc tggcgacgac aaaatcgccc agcgactgtt tttccttcat cactttttcc
    83581 atcagctgtg cgaccaggct ttcggtaaac tggccttccg gcagctgggc aatgatggtt
    83641 tcgcggatgc gctggctttc ggttttgtcg gctggcacgc tggcgggcac ggacgccgtt
    83701 accgggatcg attcatcaca ttcggtgatg gtaaaaccca gtagatccgc cacttcacgg
    83761 gcttccgggg taataatgct ggcgcgcaga accacggaca ttgcctgttc gccgcgcgcg
    83821 tgtgcttcac gaatatcatt cgctgtgatg agttttttca cctgcttcct cctgtttggt
    83881 gagagatgcc agataatcca ccagttgctg cacgctttgc gggtcgtggc tgtttagctc
    83941 gaaaatcggc tcttcgaaac ccgtctcaag caataacttt cgcgttgcgg cgacgtcagc
    84001 atctggcatg tccgttttac tgatgacggc gatttgccgt ttgctgacgc caatatccag
    84061 caacccggca ggcaaccgac tttccgggtc atttgcgcca tggacataga tcagcatgtc
    84121 tacatcctgt agcgtggtaa ttaaggcgtg ataccagcgg ggatggctaa aatattcacc
    84181 cggcgtatca atatcgccct tatcattaaa ttccacggcc agtgtttttc tggcgagggt
    84241 ataattcccc tgcaacgcat taaataacgt tgtttttcct gccccgacgc tacccacaaa
    84301 cgcaattcgt ttcatgaatg c
//
LOCUS       DAJVRL010000002        78782 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVRL010000002 DAJVRL010000000
VERSION     DAJVRL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.002"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..228
                     /locus_tag="OQN02_000086"
     CDS             <1..228
                     /locus_tag="OQN02_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCS1641034.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            648..1263
                     /locus_tag="OQN02_000087"
                     /pseudo
     CDS             648..1263
                     /locus_tag="OQN02_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1366..1689
                     /locus_tag="OQN02_000088"
     CDS             1366..1689
                     /locus_tag="OQN02_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCS1641035.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OQN02_000089"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OQN02_000089"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1641036.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OQN02_000090"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OQN02_000090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1641037.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OQN02_000091"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OQN02_000091"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1641038.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OQN02_000092"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OQN02_000092"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1641039.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OQN02_000093"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OQN02_000093"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1641040.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OQN02_000094"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OQN02_000094"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1641041.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OQN02_000095"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OQN02_000095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1641042.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OQN02_000096"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OQN02_000096"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1641043.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OQN02_000097"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OQN02_000097"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1641044.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OQN02_000098"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OQN02_000098"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1641045.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OQN02_000099"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OQN02_000099"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1641046.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OQN02_000100"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OQN02_000100"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1641047.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OQN02_000101"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OQN02_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1641048.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OQN02_000102"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OQN02_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1641049.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19269..20486
                     /gene="alaA"
                     /locus_tag="OQN02_000103"
     CDS             19269..20486
                     /gene="alaA"
                     /locus_tag="OQN02_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1641050.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20570..21169
                     /gene="yfbR"
                     /locus_tag="OQN02_000104"
     CDS             20570..21169
                     /gene="yfbR"
                     /locus_tag="OQN02_000104"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1641051.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21228..23060)
                     /locus_tag="OQN02_000105"
     CDS             complement(21228..23060)
                     /locus_tag="OQN02_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1641052.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OQN02_000106"
     CDS             complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OQN02_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCS1641053.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23808..24302)
                     /locus_tag="OQN02_000107"
     CDS             complement(23808..24302)
                     /locus_tag="OQN02_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1641054.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24385..24840)
                     /locus_tag="OQN02_000108"
     CDS             complement(24385..24840)
                     /locus_tag="OQN02_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1641055.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25178..26380
                     /gene="ackA"
                     /locus_tag="OQN02_000109"
     CDS             25178..26380
                     /gene="ackA"
                     /locus_tag="OQN02_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1641056.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26455..28599
                     /gene="pta"
                     /locus_tag="OQN02_000110"
     CDS             26455..28599
                     /gene="pta"
                     /locus_tag="OQN02_000110"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1641057.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28789..30309
                     /gene="yfcC"
                     /locus_tag="OQN02_000111"
     CDS             28789..30309
                     /gene="yfcC"
                     /locus_tag="OQN02_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1641058.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OQN02_000112"
     CDS             complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OQN02_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1641059.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OQN02_000113"
     CDS             complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OQN02_000113"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1641060.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OQN02_000114"
     CDS             complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OQN02_000114"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1641061.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32329..32976
                     /gene="yfcG"
                     /locus_tag="OQN02_000115"
     CDS             32329..32976
                     /gene="yfcG"
                     /locus_tag="OQN02_000115"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1641062.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33033..33395
                     /gene="folX"
                     /locus_tag="OQN02_000116"
     CDS             33033..33395
                     /gene="folX"
                     /locus_tag="OQN02_000116"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1641063.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33416..34309
                     /locus_tag="OQN02_000117"
     CDS             33416..34309
                     /locus_tag="OQN02_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1641064.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OQN02_000118"
     CDS             complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OQN02_000118"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1641065.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OQN02_000119"
     CDS             complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OQN02_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1641066.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36225..36941)
                     /locus_tag="OQN02_000120"
     CDS             complement(36225..36941)
                     /locus_tag="OQN02_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1641067.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OQN02_000121"
     CDS             complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OQN02_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1641068.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OQN02_000122"
     CDS             complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OQN02_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1641069.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OQN02_000123"
     CDS             complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OQN02_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS1641070.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OQN02_000124"
     CDS             complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OQN02_000124"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1641071.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OQN02_000125"
     CDS             complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OQN02_000125"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1641072.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OQN02_000126"
     CDS             complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OQN02_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1641073.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OQN02_000127"
     CDS             complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OQN02_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1641074.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OQN02_000128"
     CDS             complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OQN02_000128"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1641075.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OQN02_000129"
     CDS             complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OQN02_000129"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1641076.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45790..46449)
                     /locus_tag="OQN02_000130"
     CDS             complement(45790..46449)
                     /locus_tag="OQN02_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1641077.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OQN02_000131"
     CDS             complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OQN02_000131"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1641078.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47344..48357)
                     /locus_tag="OQN02_000132"
     CDS             complement(47344..48357)
                     /locus_tag="OQN02_000132"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1641079.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OQN02_000133"
     CDS             complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OQN02_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1641080.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49658..50653
                     /gene="flk"
                     /locus_tag="OQN02_000134"
     CDS             49658..50653
                     /gene="flk"
                     /locus_tag="OQN02_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1641081.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50650..51828)
                     /locus_tag="OQN02_000135"
     CDS             complement(50650..51828)
                     /locus_tag="OQN02_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1641082.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OQN02_000136"
     CDS             complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OQN02_000136"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1641083.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53483..55489
                     /gene="mnmC"
                     /locus_tag="OQN02_000137"
     CDS             53483..55489
                     /gene="mnmC"
                     /locus_tag="OQN02_000137"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1641084.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55610..55888)
                     /locus_tag="OQN02_000138"
     CDS             complement(55610..55888)
                     /locus_tag="OQN02_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1641085.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OQN02_000139"
     CDS             complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OQN02_000139"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1641086.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56470..57279)
                     /locus_tag="OQN02_000140"
     CDS             complement(56470..57279)
                     /locus_tag="OQN02_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1641087.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OQN02_000141"
     CDS             complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OQN02_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1641088.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OQN02_000142"
     CDS             complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OQN02_000142"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1641089.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OQN02_000143"
     CDS             complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OQN02_000143"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1641090.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60325..60876
                     /gene="smrB"
                     /locus_tag="OQN02_000144"
     CDS             60325..60876
                     /gene="smrB"
                     /locus_tag="OQN02_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1641091.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60980..61870)
                     /locus_tag="OQN02_000145"
     CDS             complement(60980..61870)
                     /locus_tag="OQN02_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1641092.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61857..62381)
                     /locus_tag="OQN02_000146"
     CDS             complement(61857..62381)
                     /locus_tag="OQN02_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1641093.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62378..62848)
                     /locus_tag="OQN02_000147"
     CDS             complement(62378..62848)
                     /locus_tag="OQN02_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1641094.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62845..63393)
                     /locus_tag="OQN02_000148"
     CDS             complement(62845..63393)
                     /locus_tag="OQN02_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1641095.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63398..64120)
                     /locus_tag="OQN02_000149"
                     /pseudo
     CDS             complement(63398..64120)
                     /locus_tag="OQN02_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64140..64862)
                     /locus_tag="OQN02_000150"
     CDS             complement(64140..64862)
                     /locus_tag="OQN02_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1641096.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64922..66154)
                     /locus_tag="OQN02_000151"
     CDS             complement(64922..66154)
                     /locus_tag="OQN02_000151"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1641097.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66203..66781)
                     /locus_tag="OQN02_000152"
                     /pseudo
     CDS             complement(66203..66781)
                     /locus_tag="OQN02_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66863..67426)
                     /locus_tag="OQN02_000153"
     CDS             complement(66863..67426)
                     /locus_tag="OQN02_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1641098.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="OQN02_000154"
     CDS             complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="OQN02_000154"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1641099.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="OQN02_000155"
                     /pseudo
     CDS             complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="OQN02_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="OQN02_000156"
     CDS             complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="OQN02_000156"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1641100.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72432..72716)
                     /locus_tag="OQN02_000157"
     CDS             complement(72432..72716)
                     /locus_tag="OQN02_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1641101.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73088..74428
                     /gene="fadL"
                     /locus_tag="OQN02_000158"
     CDS             73088..74428
                     /gene="fadL"
                     /locus_tag="OQN02_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1641102.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75179..75853
                     /locus_tag="OQN02_000159"
     CDS             75179..75853
                     /locus_tag="OQN02_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641103.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="OQN02_000160"
     CDS             complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="OQN02_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1641104.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            77084..78016
                     /locus_tag="OQN02_000161"
     CDS             77084..78016
                     /locus_tag="OQN02_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1641105.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            78090..78164
                     /locus_tag="OQN02_000162"
     tRNA            78090..78164
                     /locus_tag="OQN02_000162"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78123..78125,aa:Arg,seq:cct)
     gene            78326..>78782
                     /gene="intS"
                     /locus_tag="OQN02_000163"
     CDS             78326..>78782
                     /gene="intS"
                     /locus_tag="OQN02_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCS1641106.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRG"
BASE COUNT        19967 a        21039 c        19324 g        18452 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctataaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa aaaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc atcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac
    19141 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    19201 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    19261 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    19321 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    19381 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19441 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19501 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19561 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19621 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19681 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19741 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa
    19801 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19861 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19921 ctgagcatca ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac
    19981 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    20041 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    20101 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    20161 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    20221 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    20281 tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc
    20341 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    20401 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20461 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20521 tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20581 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20641 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20701 ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20761 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20821 cttcaatgcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact
    20881 ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc
    20941 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    21001 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    21061 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    21121 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    21181 ctgcgtcgca tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    21241 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    21301 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa
    21361 gacaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa
    21421 ggcgacactc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21481 gcggtattag agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21541 atatgtggcc cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct
    21601 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21661 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21721 caggcgatga tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt
    21781 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21841 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21901 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21961 ccttccagcg ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga
    22021 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    22081 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    22141 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    22201 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    22261 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    22321 tccagccgtt gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    22381 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22441 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22501 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22561 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22621 ctaatcagcc cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22681 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac
    22741 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22801 gccactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22861 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22921 tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22981 tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc
    23041 caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc
    23101 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    23161 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    23221 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg
    23281 ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa
    23341 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
    23401 gctcagcggt tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg
    23461 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
    23521 tgagtaaggc gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt
    23581 gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
    23641 gcaaagaagt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
    23701 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
    23761 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
    23821 atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta
    23881 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
    23941 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
    24001 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
    24061 tcctgcaaat tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata
    24121 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
    24181 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
    24241 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
    24301 attgttgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct
    24361 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
    24421 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
    24481 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
    24541 agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact
    24601 taaggcgaac agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca
    24661 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
    24721 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta
    24781 atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
    24841 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
    24901 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
    24961 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
    25021 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
    25081 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
    25141 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
    25201 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
    25261 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
    25321 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
    25381 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
    25441 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
    25501 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
    25561 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
    25621 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
    25681 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
    25741 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
    25801 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
    25861 ctctatgggt ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
    25921 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
    25981 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
    26041 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
    26101 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
    26161 tgcattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
    26221 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
    26281 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
    26341 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
    26401 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
    26461 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
    26521 gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag
    26581 ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
    26641 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
    26701 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
    26761 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
    26821 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
    26881 gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc
    26941 aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag
    27001 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
    27061 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg
    27121 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc
    27181 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
    27241 agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
    27301 cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc
    27361 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
    27421 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc
    27481 ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag
    27541 gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag
    27601 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa
    27661 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct
    27721 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
    27781 cgtgttgcag cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa
    27841 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
    27901 gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa
    27961 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
    28021 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
    28081 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
    28141 gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc
    28201 gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta
    28261 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
    28321 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac
    28381 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
    28441 acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt
    28501 atgcgcaaac cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
    28561 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt
    28621 tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt
    28681 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
    28741 cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
    28801 actgaatcca aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
    28861 gttgctattt taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag
    28921 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
    28981 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
    29041 acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    29101 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    29161 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    29221 gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta
    29281 tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg
    29341 ctgggctatg acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt
    29401 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    29461 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg
    29521 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    29581 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    29641 ggtgactggc tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg
    29701 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    29761 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    29821 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    29881 gggattttgc tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc
    29941 ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg
    30001 ttgctcttcc aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta
    30061 actatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg
    30121 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta
    30181 atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc
    30241 ctgcttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc
    30301 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    30361 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    30421 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg
    30481 acttcatctt cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag
    30541 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    30601 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga
    30661 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    30721 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    30781 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    30841 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    30901 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    30961 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    31021 ccataacccg ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa
    31081 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    31141 tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt
    31201 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga
    31261 tccacttcgc tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc
    31321 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    31381 aacacgtcgc caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca
    31441 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    31501 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    31561 cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata
    31621 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    31681 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    31741 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc
    31801 ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc
    31861 gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    31921 aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    31981 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    32041 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    32101 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc
    32161 atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg
    32221 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg
    32281 cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc
    32341 tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt
    32401 gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg
    32461 cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctgc cgatggcggc
    32521 gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    32581 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    32641 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa
    32701 accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta
    32761 aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt
    32821 gcctgctggc cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg
    32881 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta
    32941 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    33001 ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    33061 taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    33121 aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag
    33181 atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga
    33241 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    33301 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    33361 attcggtatc catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa
    33421 catagtgatc accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga
    33481 gctgggccat caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg
    33541 ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    33601 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga
    33661 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgctag
    33721 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    33781 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    33841 ccgctgggaa gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    33901 aaccggcgtg gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg
    33961 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    34021 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    34081 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca
    34141 tcgcccggct attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc
    34201 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    34261 gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca
    34321 gcaaacatcc gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc
    34381 gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat
    34441 ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa
    34501 tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc
    34561 aataaatgca cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt
    34621 tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc
    34681 caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg
    34741 ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata
    34801 tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata
    34861 aggacttctg caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc
    34921 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
    34981 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
    35041 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
    35101 tggttctagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
    35161 attaataaaa ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
    35221 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
    35281 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
    35341 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
    35401 ccccttgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
    35461 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
    35521 cttcttctat tttcccctga tggaggaaaa tgacgtgagt agaaacatgg cgagcaaagc
    35581 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
    35641 gtaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
    35701 cttctggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
    35761 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca
    35821 ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
    35881 cgttttccaa caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
    35941 gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
    36001 gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga
    36061 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
    36121 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt
    36181 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
    36241 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
    36301 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
    36361 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca
    36421 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
    36481 gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct
    36541 tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
    36601 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
    36661 aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac
    36721 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
    36781 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
    36841 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
    36901 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
    36961 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca
    37021 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
    37081 gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
    37141 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
    37201 tgatccgccg aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc
    37261 ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa
    37321 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
    37381 cctccgtcac catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca
    37441 agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
    37501 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
    37561 tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca
    37621 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
    37681 cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa
    37741 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
    37801 cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
    37861 cagtttttca tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt
    37921 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
    37981 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
    38041 ccagtgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
    38101 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc
    38161 ggcagcgtac agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag
    38221 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
    38281 gttttcgaca aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat
    38341 atcgaaacca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
    38401 ggtaccgatg cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc
    38461 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
    38521 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
    38581 aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgtatttca
    38641 atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca
    38701 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
    38761 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
    38821 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
    38881 ctgatgagcc agcaaaagcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
    38941 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
    39001 aatcctggtt ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt
    39061 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
    39121 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
    39181 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt
    39241 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acccaggtac
    39301 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
    39361 cacctttagc atctttcgac gagaacagcg cataggtggt atcggttccg atacgtaccg
    39421 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag
    39481 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
    39541 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    39601 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    39661 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    39721 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    39781 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
    39841 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata
    39901 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
    39961 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
    40021 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    40081 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
    40141 gcatcgagcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    40201 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
    40261 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
    40321 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
    40381 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
    40441 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
    40501 taacgtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    40561 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
    40621 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
    40681 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    40741 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    40801 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
    40861 gtttttatcg cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg
    40921 cagctgctgg cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg
    40981 gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg
    41041 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    41101 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
    41161 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    41221 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    41281 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    41341 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
    41401 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    41461 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
    41521 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    41581 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    41641 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    41701 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    41761 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    41821 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    41881 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    41941 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa
    42001 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat
    42061 cagctgtgat ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa
    42121 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    42181 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    42241 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    42301 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    42361 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgtgga tcaggctaac
    42421 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
    42481 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    42541 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    42601 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
    42661 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
    42721 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
    42781 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
    42841 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
    42901 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
    42961 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
    43021 cgcaggtggt gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc
    43081 gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag
    43141 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
    43201 cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcacgatcgc ccgctttcgg
    43261 caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc
    43321 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
    43381 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
    43441 aagaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg
    43501 cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
    43561 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
    43621 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
    43681 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
    43741 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
    43801 cgctggcaat cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg
    43861 ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
    43921 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
    43981 cccgacgttg taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt
    44041 ccggctcacc gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa
    44101 tactttcgcg atctggaccc agccagtcgg tatgatccag cgcaatactg gttactaccg
    44161 cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
    44221 tcaccacgtc aagttgtgcc tgcttgaaca gccacaacgc cgacagcgta ccgtactcga
    44281 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
    44341 ccgattccgg caattcctgg ccctgcacac gtacgcgctc ggtataacgc accagatgag
    44401 gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
    44461 aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagga
    44521 cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
    44581 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
    44641 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
    44701 gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta
    44761 cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc
    44821 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
    44881 tcaggaattc actgcgctgg aatctaggcg gcagtttttc gcgaacggtc tgttcgataa
    44941 cacgcggacc ggcaaagccg attaacgctt taggttcagc gatgttgaga tcgcccagca
    45001 tggcgaagct tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca
    45061 agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca
    45121 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat
    45181 cttcctgcgc ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac
    45241 cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt
    45301 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt
    45361 tatacttctt ggagtcacga aacttcagct catctttcgg ctcaagctcg ctacccagct
    45421 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    45481 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    45541 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    45601 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    45661 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    45721 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    45781 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tgacggatta tttcgatgac
    45841 gcccggcaaa atagaaacca caataatccc gacgatcagc agcttaaggt tatcctgaac
    45901 catcgggatt gtaccgaaga aatagcctgc gtaggtaaaa agcagtaccc acaacagtgc
    45961 gccgatcacg ttataagcag cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa
    46021 cggggcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg
    46081 tttctcataa aactgatggg ttttgtcgag ataactgcga cggaaaattt tcgaattggg
    46141 attactgaat aacttttcac cgaacaaccg cccgatagtg taattgaccg cgtcacccac
    46201 aatcgcggcg atcagcatca gcactaccat catatggacg ttgagatcgt tggtttccag
    46261 tgatgccaac gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcattaccac
    46321 cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga cgccgtactc
    46381 cgcgactagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    46441 caggtccata tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    46501 acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg
    46561 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    46621 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    46681 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    46741 gatgtacaaa ggcattcgct ttgatatcaa ccaccacata aggaccgtga cgcgtgacat
    46801 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    46861 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    46921 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    46981 tgatgtagcg ataacggcga gccgtggcgc taaatcgtgc atgaaaatca tcaggtacag
    47041 ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc aaggtccacg
    47101 ccgcgtcttt gcgctgcgcg gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc
    47161 ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    47221 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact
    47281 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg
    47341 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    47401 gccaaagcga acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc
    47461 attacgcacg cagccaacag aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg
    47521 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    47581 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    47641 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    47701 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag
    47761 catattgaac gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag
    47821 caatttcgca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    47881 aatcaggctg gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc
    47941 cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc
    48001 ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa
    48061 cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc
    48121 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc
    48181 ggcatcctgc acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    48241 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    48301 ggcttcgccc atagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    48361 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    48421 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    48481 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    48541 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg
    48601 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc
    48661 agcgtaatgc ggccaaactc aggcgcgggc agcaacgtat ccagcgcaac gtgttgttca
    48721 tgcccaataa acttgctata ggcttcaaac acttgcgtcg tgccacgcgc tttaccctcc
    48781 agggtatagc ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta
    48841 agttccggct cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg
    48901 caggtcagca gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    48961 ccgggcttca ggctacggat cagtttttca tccgccagat gtagcgtttt gtacggacca
    49021 tctttaaaga gtggcgtatg gaaagtcaga atatccgcgc gctggactaa ctcatccagc
    49081 gagcggaaat caccctcatc cccacggtcg gcgcgaggcg gatcgcaaag taaggtttta
    49141 atccctaacg cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc
    49201 cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag cagggaggag
    49261 aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    49321 gcctgcttca accatgcttc atcgacatgg tcggtccccg cagtggcagt tccaacaaat
    49381 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    49441 gcatccgcgt ctgccagttg agcgacgggg attggacgcc cgggaaccgc gatcacctca
    49501 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg
    49561 tttgtgttac ctgtatgaga cgagagttaa ccggacaagt atgccataat ctcgcggcca
    49621 ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc
    49681 tcctcctggg cagccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc
    49741 tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac
    49801 gcaacaacaa agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga
    49861 ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct
    49921 ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg
    49981 tctggggaat catcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc
    50041 gctgaatcag ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa
    50101 gatggttatt ccgcaaccac agcagcgcga ggcgaccgac cgtcctttat taccggcgga
    50161 gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa
    50221 acagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc
    50281 gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa
    50341 tacgccgacg ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact
    50401 ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc
    50461 ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga
    50521 tccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa
    50581 tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg
    50641 gctggttcgt taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga
    50701 gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag
    50761 cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc
    50821 gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga
    50881 acatccggct ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca
    50941 gagcagcaac aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc
    51001 aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc
    51061 ggtaagagta aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt
    51121 cccgataacc gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag
    51181 ccccacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt
    51241 gccgttacag gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa
    51301 accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat
    51361 cgccattccg ttccatgaca tcactttgcc agagtgtttt ggccctacga tgcctaaccc
    51421 ccaggtcaga gcgcctgtca gtaactggct ttcaccaaac ccaagaatca aacgcccgat
    51481 gaccaacagg gcaaatttga acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc
    51541 gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg cgccatattg
    51601 atcggccagt cgcccggcgt aaccacgcgt cagcaccgta gccagaaact gaatcccgac
    51661 ggcaatgccg accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac
    51721 cggcaacggc aaccctacgg tcatgtaggt gagaaaaacc gcaaaagaga tgcggaagag
    51781 cgaaaaactg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag
    51841 aatgcagcgc aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacggtgc
    51901 gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc
    51961 ctagaactgg tggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg
    52021 ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg
    52081 acctactccg gcctactgcg aattaatctt ttcagcttgc gcattaccag cgtggcgttg
    52141 gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg
    52201 gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg
    52261 ataaagccgt gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc
    52321 agtgagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    52381 tcacggatag ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc
    52441 aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct
    52501 tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca
    52561 gcatagatgt aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg
    52621 ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc
    52681 ggggtgtcgt tgtatttagt agatagcgca cccatcgcgt cgaattcgca ggccatttcc
    52741 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    52801 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    52861 acgccatgaa ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta
    52921 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    52981 aacacctgga aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta
    53041 ttctggtaag cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat
    53101 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    53161 tccagtttta tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag
    53221 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    53281 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    53341 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    53401 atcagccatt taatagagat atttgcgcag ccttacacac atcgctaaga tcgagccacc
    53461 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    53521 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    53581 cgggctggaa gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc
    53641 tgagcatcca catcctctgt ttgtgatagc agagagcggc ttcggcaccg gattaaactt
    53701 cctgacgcta tggaaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    53761 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    53821 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    53881 gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg
    53941 gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga
    54001 tgcctggttt ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct
    54061 gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc
    54121 cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt
    54181 tgggcgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc
    54241 cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg
    54301 tattgccagc gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta
    54361 ttgcgcggat gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc
    54421 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc
    54481 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    54541 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc
    54601 cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    54661 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    54721 taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    54781 gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    54841 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac
    54901 tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc
    54961 agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    55021 acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    55081 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa
    55141 agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    55201 tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt
    55261 ggcggagcaa aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc
    55321 ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca
    55381 gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg
    55441 gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc
    55501 agtgtttgcc ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga
    55561 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg
    55621 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    55681 attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    55741 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    55801 acccactggc aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca
    55861 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctgac
    55921 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    55981 aattttgtag tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat
    56041 ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt
    56101 ccagattgtc gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga
    56161 acgcctgcgg cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt
    56221 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    56281 agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat
    56341 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    56401 tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg
    56461 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    56521 taatttggca ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc
    56581 tttgctcaac accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac
    56641 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt
    56701 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    56761 cataacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa
    56821 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    56881 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg
    56941 caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga
    57001 gccgacaaag gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    57061 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    57121 cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc
    57181 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    57241 cagcggagaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    57301 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc
    57361 ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt
    57421 aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc
    57481 gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    57541 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    57601 gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta
    57661 ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    57721 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    57781 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    57841 agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa
    57901 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca
    57961 gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    58021 tgggttattt tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc
    58081 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    58141 ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg
    58201 atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact
    58261 tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    58321 gccatatgag caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg
    58381 ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact
    58441 acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    58501 gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg
    58561 gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt
    58621 tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc
    58681 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca
    58741 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    58801 gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg
    58861 taggtgtaat cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa
    58921 cgctggtcgg tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa
    58981 acaccggaga gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc
    59041 ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc
    59101 ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg
    59161 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    59221 gtttacttaa tctttataaa tcgcgaaatg ttctcgtgcg gcaataagct gctctttagt
    59281 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg
    59341 atattgttcc ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc
    59401 agcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc
    59461 agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    59521 cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg
    59581 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    59641 ttcggcaacc gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa
    59701 ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg
    59761 cggttgcttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg
    59821 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt
    59881 caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg
    59941 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta
    60001 gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta
    60061 ccagatattt gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg
    60121 cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    60181 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    60241 tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg
    60301 aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    60361 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac
    60421 cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    60481 gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg
    60541 aaatatgttc gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat
    60601 tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg
    60661 ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg
    60721 cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg
    60781 atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt
    60841 gaagtggaag aatggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca
    60901 gcgtaaacgc cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc
    60961 gtaggcctga taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact
    61021 gaaaacgaca cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat
    61081 cgtgtagtac tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat
    61141 cacaaccgta agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc
    61201 catcattatc agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg
    61261 aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga
    61321 cataattttc actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt
    61381 tatgcactgt aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg
    61441 atgcgatcca agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg
    61501 cacaacgaaa aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt
    61561 cagtcagtac caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat
    61621 cgcctttata cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg
    61681 taacattgaa aacactgctc gctacagcac cggttacctt gtccatggca taataaactc
    61741 tcacactgtc aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga
    61801 caacactcag aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag
    61861 aaatactcaa aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg
    61921 ctctttgcct gtaaccaggt attaaaattt aggctgttat ttccctgatt cagcgggagc
    61981 gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc
    62041 ccagcgccgg atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct
    62101 tctgttcctg tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat
    62161 tgaaacacca atggcactcg ctctgactgc ccaaggttca ttaaatcact ggcggcaatg
    62221 gcaggaaaat gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg
    62281 attaaattgc cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac
    62341 atcaggctac taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg
    62401 tggcccaggc ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag
    62461 ccgttaacac cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct
    62521 gccctgcctg tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat
    62581 caaaatcgct tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg
    62641 ccgtatcgca ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat
    62701 caataagcac atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag
    62761 gcggctgcac taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc
    62821 acagcaaaat aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt
    62881 aaattcagct gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg
    62941 aactcccgag gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg
    63001 atcactactt ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc
    63061 accgttaatc acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt
    63121 gttacaaaca agcgagtagt caatgggacg tttataatca accctattga tcttattaat
    63181 gaatacatta ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac
    63241 aagcacccgt aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac
    63301 acaacatatc agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct
    63361 cccggcctca gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag
    63421 tgaaaaagag cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac
    63481 ccatcttcac attcagcggt gcagaggttt tcggcgacat cactattagc gagaaaccgg
    63541 ctgccggatt gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg
    63601 ggttattgac ggtaaattcc tgaccgttgc gggttaaagt aatcttatat tgctaggggt
    63661 ttttcatgct gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta
    63721 gtgctatttg cagcgtgttg gctttgtttg attttggcgg gatctcgcgc acgttgaagt
    63781 agaacaggct ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta
    63841 cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac
    63901 tgatcttgtt acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat
    63961 cgttattgcg caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac
    64021 gatctggcgt tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg
    64081 tcgtagcacc caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt
    64141 tattggttag ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc
    64201 tgaatgacag gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat
    64261 ggcagtaaca agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa
    64321 tgcgcaacac cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa
    64381 acattgccgt catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg
    64441 ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg
    64501 atgacggcaa atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac
    64561 tgggttgctt cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa
    64621 tagttattga cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg
    64681 ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc
    64741 atattctggg tacggtgaag tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac
    64801 gacaagcccg cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc
    64861 agcgaaccat catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg
    64921 ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc
    64981 gtcccactgc catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga
    65041 gcgaaatgta catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg
    65101 agatgctgac attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt
    65161 ttgtctgctc ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac
    65221 ccgcatcgcg gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc
    65281 gtaccattcc gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg
    65341 agaagcgata tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac
    65401 tcacacggaa ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt
    65461 tggtgtgcga gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga
    65521 caccaagcgc cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc
    65581 cgttagcgat cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc
    65641 cccactcttg cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt
    65701 atggcatcga ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc
    65761 gaatatgcaa cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg
    65821 gaacctgcgt ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt
    65881 gtgccacgcc ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt
    65941 cagtactgac actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat
    66001 cctcgcccag cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac
    66061 tccactccca ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag
    66121 tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa
    66181 ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt
    66241 gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg
    66301 gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg
    66361 actggcttaa atcagcctta atttccatgc cttccagttg accggatttc aggcatttag
    66421 catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca
    66481 gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa
    66541 tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg
    66601 gctcaacata tccctagctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt
    66661 ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg
    66721 acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca
    66781 tacacgtttc tatccgtttt tattcttgtt cggtcaaaat acatttaaat cccccatagc
    66841 agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa
    66901 aattacccag atctggcgca tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct
    66961 gcttcgcttt gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc
    67021 cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg
    67081 caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac
    67141 cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct
    67201 ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca
    67261 tatcaacttc cagtttgtga tcgtctggaa cgatagaaca cactgagtct tcaatggtgc
    67321 cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc
    67381 ccatcaccat tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa
    67441 ctataaataa ttaaaaatat aaacattgca atacattgat tcagtcaata gccaatgttt
    67501 atacccagcc attgaatacg gcatgcgacc caaaaagaac aggtcgtaga aaattgtatt
    67561 cattggtgtg gggccgcata gtgatgtttc cacacccctg atccaattta attttactaa
    67621 agagtaaatg tagatttaat taatatattg acagaagaaa ggttatctta aggacatcct
    67681 taaaaaatac ggaataagta tttagaaaaa atagaaagta tatcaataat gaatatcaac
    67741 agcttaaaaa acaaacaatg aacatataat gcgattggca tttatccact tttgacaata
    67801 taatatataa acactatagc cagaatttaa tcaagtttca attacatcct gcctgattat
    67861 tcaactctat tttctctatt atctgtatta ataaatgaca atgagtgtca aacagatatt
    67921 attcatttca acatgtaact ttgggaaaaa tcaagatacg cgcagaagaa taatttccga
    67981 atgaatgtag aaagtgtttt atttatagat aaggagagtc ttcgatagta gtgaggttga
    68041 cagatatccg ctgcctaatg agcaattatg cccaatgaat ctacctcatt gggcataatt
    68101 tggaacacat cagatagctt ttgccatctt cagattacac gggctcatct gccagttgaa
    68161 tgtaccgttt ccgctttcat caagcgttac gctggcaatg gccgaggtgg taaacatagg
    68221 cggcgtttcg cccggacata actcggcaac cagatacccg actaacggta agtgagagat
    68281 caccagtacc gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc
    68341 aacatcgccg cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca
    68401 atcccctact tcttccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc
    68461 gatttccact ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt
    68521 agtggtcaga ggacgaaccg aatcactggc ggaatcgagg gctgcgtcgc cgtgacgcat
    68581 gataaaaact tgcatattgc accgcttttg ttaaccagat tcgcccgttt tctttcatct
    68641 gaaaccagag aaagaaaacg gtggccggca ttgtgcctta tccattcacc gaatgaaacg
    68701 ctgtttttta cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaacact
    68761 ccgccattca gcgcggattc atatagcttt gaccttctta ttgcaggtca gttgcagttg
    68821 ttttccaaaa actttcccca cgcgcgccca tctcgaccaa acgctcgcaa ggggtaaaac
    68881 gggaaccata ctgcgtggcc agtcgttgca ttattgcaac cacttcgccc gcgccgagag
    68941 aatcgatata gcggaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac
    69001 caatatcccc gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat
    69061 tcagcatcaa catcacacac cgttcagcaa cctgcggtgc ggagagtcac ccctgccctt
    69121 gtgcgccaat gagcgggtaa atggcaggat cgacctgttt tttgctttta cgccctttct
    69181 gaccataaag atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg
    69241 aagaaacaac atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa
    69301 taattttagt cccggtgtcg attcctacct catccaaaag ttggattggg cctaccggaa
    69361 aaccaaattt cactagcgcg gcatcaatgt gctctacccg ttcaccttcg gtcagcatgc
    69421 ggatagcttc attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac
    69481 gcacgacaat gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt
    69541 gcgccgatgt acccgcatga ggaataatct ctaccagcgg cattttttcc accggactga
    69601 agaaatgcag gccaataact tgctcaggtc gcgcggcgtg agcggcgata tcaccaatcg
    69661 gtaaagatga cgtgttcgaa gcaaaaatgg tatgagcggc gcaattttgc tccacttccg
    69721 ccaccatctg ttgttttaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat
    69781 gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcgc
    69841 tggctttgag atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg
    69901 catgatttat gccctgcggg ttgatatctt taattctgac cggaagcccc gctttacaag
    69961 cagtgacata agcaataccg ccgcccatca agccaccacc taaaattccc acgctgttta
    70021 atgccgcagg cggtgcatca ctaccgggat ctttcttcac gtccgtactg gcaaaaaaga
    70081 tactacgcaa agcttgcgat tgcggcgtca tcgccagttc gccaaaggcg cgagcttcag
    70141 cgtcatagcc gctgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt
    70201 ctgtcgccgg ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca
    70261 gcagcgcacg acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag
    70321 atgggcgatc cttcttcgcc agctcaacag cggcttccag caaaatggag tgcggaacaa
    70381 cgtcatccac cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagaa
    70441 tcatctctaa tgctgtactg acgcctatca gacgcggtaa acgctgggtg ccgcctgaac
    70501 cgggtaacaa tccaagttgt acttcagaca aaccgagcac cgttttagga tcgtcagtac
    70561 aaacgcgacc gtggcacgcc agcgccagct ccagcccacc gcccaggcaa gcgccatgaa
    70621 tagccgcgat aaccggaatg ggcaaagcat gaatctccgc catcaactgt tggccctgcc
    70681 gcgccagtgc ttccgcttct tgcgccgttt tgcagttgcc aatcatgttg atgtctgctc
    70741 cggcgataaa attgtccggt ttcgcggaga taaacaccac gccgcgcaac tctttgtttt
    70801 cacggagttg cttaataatg gcgcgcacct gcgaggcaaa ttccgccttc agggtattca
    70861 ttttctcatc cggtacgtcg atggtgatga tagcaatgtt gtccagacga acattaagag
    70921 taaacgctga tgccatttcc attattccgc ctccagaacc attgccgcgc caagcccacc
    70981 ggcagcacag gcggtaacta aaccaaatcc accgccgcga cggcgaagtt catgcaatgt
    71041 ctgggtaatc atccgcgcgc cggtcgccgc gaagggatgt ccgtaagcaa tcgaaccgcc
    71101 aagcacgtta aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc
    71161 acgagcaaaa cgttcactac ccagcaactg aatattcgcc agtgtctgag ctgcaaaggc
    71221 ttcgtgcata tcgatcaatg tcagttcgct catcgtcaaa ccggcacgtt ccagcgccag
    71281 cggtgttgac caggctggac cgagcaacat gtcctgccag acatcaatcg cagtaaatgc
    71341 gtagctgcgc agatacccca gcggcaccag ccctaattct ttcgcccggg attcagtcat
    71401 caggatcacc gctgccgcgc catcggtcag cggcgtactg tttgccgccg ttaccgttcc
    71461 gtgtttgcga tcaaacgccg ggcgcagctt tgcgtaatcg gcaagcgagg aattaccgcg
    71521 aatattgttg tcttcgacaa gcggttgttt ataaggaggg ataaaggcag tcatcacctc
    71581 ttctttgagt tttccgtctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa
    71641 tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc atttgttcgg cggtgtcgcc
    71701 catccgcaag ccagtagaat attctgctac cgcaggcggt acgggcatta agtcgcgcaa
    71761 acgcaggcga gagaagagtt tcaggcgctg gctcatggtt cgagctttgt tgacatcaac
    71821 cagcacgtgc gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc
    71881 accggcaatc cccgctcgga tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc
    71941 ctggaaactg gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc
    72001 ggtaccgaga acaatttcac gcgcaatgtt gggggcttca ggcatttgta cgacctgacc
    72061 aaagaccagc tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac
    72121 caccatcttc cctaaatcaa ccgtgggaat gccatgaaaa gccgtcgcct ggcgggcaaa
    72181 aggcgtacgt aaaccgctaa caatggcgat acgatcgccc tggcgggtaa ccagtggtaa
    72241 aacctgaccc ataacactcc cctgtaaaaa aataaataaa gtggtctgac ctgatcatag
    72301 tcttaaccat ttttttacat ttagccaagc ggagaaaaag gaaagtggga gctatgacac
    72361 agtgaagaca gaagaaagaa aagaaaacgc ccctgccagc aactgacagg ggcgtacgta
    72421 cccgagagga attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa
    72481 gtgaaatcga tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggagattttg
    72541 cacggttcgg attccacgct acgggatttt tcagtcagcg ccgccagcgt ttgttctgct
    72601 tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg
    72661 gtgccaacat ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt
    72721 gatttcctct gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc
    72781 ggaaagtgct gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt
    72841 aaaaatgatc taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc
    72901 agctggatcg cgtttcttag atcatatttg aaaaaagatg gaaacatact tgcaacattc
    72961 tagctggtcc gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct
    73021 acacttcgcg ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt
    73081 tatggtcatg agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact
    73141 tatctccacc caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct
    73201 gggccgggct tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa
    73261 ccccgcattg attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga
    73321 cccggatgtg aatatcagcg gaacgtctcc gtctggtcgt agcctgaaag ccgataacat
    73381 cgcgcctacg gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg
    73441 ttggggcgct tctattacct ctaactatgg tctggcaaca gagtttaatg atacttatgc
    73501 aggcggctct gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc
    73561 gtatcgctta aataatgcat ggagctttgg tcttggtttc aacgccatct acgctcgcgc
    73621 gaaaattgaa cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc
    73681 tcctgccggg caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag
    73741 taataccaaa atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat
    73801 cctgtatgaa ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat
    73861 tgacttcaaa ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc
    73921 aattcctacc gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga
    73981 aatgtgggaa gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct
    74041 ggcttacacc agctggagtc agttccagca gctgaaagcg acctcaacca gtggcgacac
    74101 gctgttccag aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta
    74161 ttactacgat gataactgga ccttccgtac cggtatcgcc tttgatgaca gcccagttcc
    74221 ggctcagaat cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac
    74281 gacttacgca tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca
    74341 gagcgtgaaa attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt
    74401 cggtactaac tttaactacg cgttctgata acgcgttcgc ctagataaag tcacctgcta
    74461 tgcaggtgac tttaactccc ccacttcacc gaaagtagtt tcctcattgc ttgcctcctg
    74521 agttttgcaa acagcctgat ggcagcttgc cctttcaaat cagtaagtag tatccacaga
    74581 caaacctgaa gggaaatgca tattttcaag cgttctgctc gtccttctca aagagtttac
    74641 ttttctgcat ttccaggata ctcaaccccc ctggctattg tgcgcttata cactcaaatt
    74701 aaagataggt tctaaataaa tgagcgtttt ttgatagtct atttcattag gtaatatata
    74761 tttgtaacaa atcaatcaac atggaataaa atcatgctac catctatttc aatcaacaat
    74821 accagcgcag cttacccaga atccatcaat gaaaataaca atgatgaaat taatggatta
    74881 gtacaagagt tcaaaaacca tttttaatgg taaggaagga ataagcacct gtattaaaca
    74941 tctacttgag cttataaaaa acgccatacg agtaaacgac gatccttata gatctaatat
    75001 taataattcc tcagttactt atattgatat tggctccaat gatacagacc atattactat
    75061 tggtatcgac aaccaagaac caatagaatt acctgcgaac tataaagaca aagaactcgt
    75121 ccgtactatc attaatgaca acatagttga gaagactcat gatatcaata acaaggaaat
    75181 gatcttcagc gcattaaaag aaatatatga tggagatcct ggttttatct tcgataagat
    75241 atcacacaaa ctcagacata cggtaacgga atttgatgag agcgggaaaa gcgaaccaac
    75301 ggacttattt acctggtacg gtaaagataa aaaaggcgac tctctcgcta ttgtaattaa
    75361 aaataaaaac ggaaatgatt acttatttct cggttactac gatcaggacg actaccacat
    75421 tcaaagagga attcgtatta atggtgatag tctcacccaa tattgtagtg aaaacgccag
    75481 gagtgcttca gcgtggtttg aaagcagtaa agctatcatg gcagaatcat ttgcaactgg
    75541 ttccgatcat caggttgtaa acgagctcaa cggggaaaga ctgagagaac ctaacgaagt
    75601 ttttaaacgt ttaggtcgag caataagata taattttcaa gtggacgatg caaaatttag
    75661 acgcgataat gtaaaagaaa taatttctac tttattcgct aacaaagttg acgttgacca
    75721 tcctgaaaat aaatacaaag actttaagaa tctcgaagat aaagttgaaa aaagacttca
    75781 aaatcgccag acgaaatatc aaaatgaaat taatcaacta tctgcactag gtgttaattt
    75841 tgatgatatt taaaattatt tttccagttt tttgattttt caacgcctga atatcggaaa
    75901 tagtcagtga caaacatcag tatttttaca tcattacgtc cgggtcattg tcggtttatc
    75961 tccttttact tgtggtaatt gagggataac aacaaaaaag gtgagttttg cgactcacct
    76021 tttttatttg tttcttattc agaatcgata tcttttaaat catcctgaat cgcttgcgcg
    76081 ttcggatttt cctgcggttt gagttcgccg ccattagcga tgaaatcatg acgctggaag
    76141 tacgcttcgc gcaccataat ataaggatcg gacgactgac gcagcagacc atcggaatcc
    76201 agcagctgag cgcgggtttc gatcccttca agcgtccatt tacccacaga catcggccag
    76261 gtcagccagg aaagaaccgg gtaaagagca tccgccatat caccaccgtc atcacgcagc
    76321 gtgaagctac cgtagaacgg taactgaacg taaggcccat aacccacgcc ataatgacca
    76381 agcgtactac cgaagcggtg aggttcagtc cgttgcagtt tcgggttcgc catccctgca
    76441 acatcaataa agccgcccat ccccaaaatg gtgttcagga aaaagcgggt aaagtggacc
    76501 atcccctgat aagggtcgcc ctgcaagaag tagttaacca tcaccgcagg ttcttcaagg
    76561 ttgccagtaa agttgctcaa accattacgc gccggttgcg gaacataatc acgccaggcg
    76621 acagcgaccg gtcgaacaat atacgggtct aatacgttga agttgaagtt gtacatggtg
    76681 cggttgaacc cttctaacgg gtcagaacgc ccttgctgat ctgtaccgga actcgcacac
    76741 cccaccagaa gcgtagttcc cagagcaagc gccgacaggc gaagcttcat aaatgtctcc
    76801 ctgttttttt atggcttatg cagtttgcca tccatgacgg aacgataccg tatccgcctg
    76861 tttaggtgtg ggcgattgta acagcacgtc aactgatgtc cagacgccct gatttgctga
    76921 tttgatcata gcctggtaat cgccgccctg taggctactt gattctatag aaacagaaaa
    76981 aggcaaacgc agccttttct acattttcag agtaactccc gccagttgcg agcaaaaaag
    77041 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
    77101 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
    77161 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
    77221 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
    77281 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgccg aactggaagg
    77341 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
    77401 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
    77461 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
    77521 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
    77581 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
    77641 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
    77701 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
    77761 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
    77821 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
    77881 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
    77941 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
    78001 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
    78061 agtccattag cgggtatact cgccgcattg tcctcttagt taaatggata taacgagccc
    78121 ctcctaaggg ctaattgcag gttcgattcc tgcaggggac accatttatc agttcgctcc
    78181 catccgtacc agtccgcaaa atcccctgaa tatcaagcct tccgtatatt cacagttcgt
    78241 catggttcgc gtcagatcgt tgacagccgc actccatgac gggtaaaaag tggataaaat
    78301 aattttaccc accggatttt tacccatgct caccgttaag cagattgaag cagcaaagcc
    78361 gaaagaaaaa ccataccgcc ttctcgatgg taatggcctg tacctttatg tccctgtatc
    78421 cgggaaaaag gtatggcagc ttcgctacaa gattgacggt aaggagaaaa tcctgaccgt
    78481 cggaaaatat ccgcttatga ctttgcagga ggcaagggat aaagcatgga ctgcgaggaa
    78541 agacatctcg gctggcatcg atccggtaaa ggcgaaaaag gcttcgtcta acaacaattc
    78601 atttagtgcg atttacaagg aatggtacga acacaagaag caagtctggt cagtagggta
    78661 tgcgactgaa cttgcaaaaa tgtttgatga cgacatttta cccatcatcg gcggccttga
    78721 gattcaggat attgagccga tgcaactgct ggaagtaatc cgcagatttg aagatcgcgg
    78781 tg
//
LOCUS       DAJVRL010000003        75869 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVRL010000003 DAJVRL010000000
VERSION     DAJVRL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.003"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..208)
                     /locus_tag="OQN02_000164"
     CDS             complement(<1..208)
                     /locus_tag="OQN02_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641107.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDR"
     gene            complement(210..430)
                     /locus_tag="OQN02_000165"
                     /pseudo
     CDS             complement(210..430)
                     /locus_tag="OQN02_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(423..644)
                     /locus_tag="OQN02_000166"
     CDS             complement(423..644)
                     /locus_tag="OQN02_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641108.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(634..834)
                     /locus_tag="OQN02_000167"
     CDS             complement(634..834)
                     /locus_tag="OQN02_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1641109.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            964..1143
                     /locus_tag="OQN02_000168"
     CDS             964..1143
                     /locus_tag="OQN02_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641110.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1268..1597
                     /locus_tag="OQN02_000169"
     CDS             1268..1597
                     /locus_tag="OQN02_000169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641111.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1647..1835)
                     /locus_tag="OQN02_000170"
     CDS             complement(1647..1835)
                     /locus_tag="OQN02_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1641112.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2210..3438)
                     /locus_tag="OQN02_000171"
                     /pseudo
     CDS             complement(2210..3438)
                     /locus_tag="OQN02_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3687..4808
                     /gene="roxA"
                     /locus_tag="OQN02_000172"
                     /gene_synonym="ycfD"
     CDS             3687..4808
                     /gene="roxA"
                     /locus_tag="OQN02_000172"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1641113.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4857..6083)
                     /gene="pepT"
                     /locus_tag="OQN02_000173"
     CDS             complement(4857..6083)
                     /gene="pepT"
                     /locus_tag="OQN02_000173"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1641114.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6333..7469
                     /gene="potA"
                     /locus_tag="OQN02_000174"
     CDS             6333..7469
                     /gene="potA"
                     /locus_tag="OQN02_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1641115.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7453..8310
                     /gene="potB"
                     /locus_tag="OQN02_000175"
     CDS             7453..8310
                     /gene="potB"
                     /locus_tag="OQN02_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1641116.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8307..9101
                     /gene="potC"
                     /locus_tag="OQN02_000176"
     CDS             8307..9101
                     /gene="potC"
                     /locus_tag="OQN02_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1641117.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9098..10144
                     /gene="potD"
                     /locus_tag="OQN02_000177"
     CDS             9098..10144
                     /gene="potD"
                     /locus_tag="OQN02_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1641118.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10300..11121)
                     /gene="cobB"
                     /locus_tag="OQN02_000178"
     CDS             complement(10300..11121)
                     /gene="cobB"
                     /locus_tag="OQN02_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1641119.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11137..12048)
                     /gene="nagK"
                     /locus_tag="OQN02_000179"
     CDS             complement(11137..12048)
                     /gene="nagK"
                     /locus_tag="OQN02_000179"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1641120.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12077..13321)
                     /gene="lolE"
                     /locus_tag="OQN02_000180"
     CDS             complement(12077..13321)
                     /gene="lolE"
                     /locus_tag="OQN02_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1641121.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13321..14022)
                     /gene="lolD"
                     /locus_tag="OQN02_000181"
     CDS             complement(13321..14022)
                     /gene="lolD"
                     /locus_tag="OQN02_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1641122.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14015..15214)
                     /gene="lolC"
                     /locus_tag="OQN02_000182"
     CDS             complement(14015..15214)
                     /gene="lolC"
                     /locus_tag="OQN02_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1641123.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15476..16549
                     /locus_tag="OQN02_000183"
     CDS             15476..16549
                     /locus_tag="OQN02_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1641124.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16677..20123
                     /gene="mfd"
                     /locus_tag="OQN02_000184"
     CDS             16677..20123
                     /gene="mfd"
                     /locus_tag="OQN02_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1641125.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20266..21228
                     /gene="ldtC"
                     /locus_tag="OQN02_000185"
     CDS             20266..21228
                     /gene="ldtC"
                     /locus_tag="OQN02_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCS1641126.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21311..21523)
                     /locus_tag="OQN02_000186"
     CDS             complement(21311..21523)
                     /locus_tag="OQN02_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1641127.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21808..22440
                     /gene="comR"
                     /locus_tag="OQN02_000187"
     CDS             21808..22440
                     /gene="comR"
                     /locus_tag="OQN02_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1641128.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22502..23041)
                     /locus_tag="OQN02_000188"
     CDS             complement(22502..23041)
                     /locus_tag="OQN02_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1641129.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23268..24572)
                     /gene="ndh"
                     /locus_tag="OQN02_000189"
     CDS             complement(23268..24572)
                     /gene="ndh"
                     /locus_tag="OQN02_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1641130.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24980..25522)
                     /locus_tag="OQN02_000190"
     CDS             complement(24980..25522)
                     /locus_tag="OQN02_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1641131.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25545..26570)
                     /gene="nagZ"
                     /locus_tag="OQN02_000191"
     CDS             complement(25545..26570)
                     /gene="nagZ"
                     /locus_tag="OQN02_000191"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1641132.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26581..27405)
                     /gene="thiK"
                     /locus_tag="OQN02_000192"
     CDS             complement(26581..27405)
                     /gene="thiK"
                     /locus_tag="OQN02_000192"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1641133.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27386..28027)
                     /gene="lpoB"
                     /locus_tag="OQN02_000193"
     CDS             complement(27386..28027)
                     /gene="lpoB"
                     /locus_tag="OQN02_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1641134.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(28042..28419)
                     /locus_tag="OQN02_000194"
     CDS             complement(28042..28419)
                     /locus_tag="OQN02_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1641135.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28422..28781)
                     /gene="hinT"
                     /locus_tag="OQN02_000195"
     CDS             complement(28422..28781)
                     /gene="hinT"
                     /locus_tag="OQN02_000195"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1641136.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            29120..31305
                     /gene="fhuE"
                     /locus_tag="OQN02_000196"
                     /pseudo
     CDS             29120..31305
                     /gene="fhuE"
                     /locus_tag="OQN02_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31365..32798)
                     /gene="ptsG"
                     /locus_tag="OQN02_000197"
     CDS             complement(31365..32798)
                     /gene="ptsG"
                     /locus_tag="OQN02_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1641137.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33093..33890)
                     /locus_tag="OQN02_000198"
     CDS             complement(33093..33890)
                     /locus_tag="OQN02_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1641138.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33901..34905)
                     /gene="holB"
                     /locus_tag="OQN02_000199"
     CDS             complement(33901..34905)
                     /gene="holB"
                     /locus_tag="OQN02_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1641139.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34902..35543)
                     /locus_tag="OQN02_000200"
     CDS             complement(34902..35543)
                     /locus_tag="OQN02_000200"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1641140.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35533..36555)
                     /gene="yceG"
                     /locus_tag="OQN02_000201"
     CDS             complement(35533..36555)
                     /gene="yceG"
                     /locus_tag="OQN02_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1641141.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36558..37367)
                     /gene="pabC"
                     /locus_tag="OQN02_000202"
     CDS             complement(36558..37367)
                     /gene="pabC"
                     /locus_tag="OQN02_000202"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1641142.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37487..38728)
                     /gene="fabF"
                     /locus_tag="OQN02_000203"
     CDS             complement(37487..38728)
                     /gene="fabF"
                     /locus_tag="OQN02_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1641143.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38815..39051)
                     /gene="acpP"
                     /locus_tag="OQN02_000204"
     CDS             complement(38815..39051)
                     /gene="acpP"
                     /locus_tag="OQN02_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1641144.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39262..39996)
                     /gene="fabG"
                     /locus_tag="OQN02_000205"
     CDS             complement(39262..39996)
                     /gene="fabG"
                     /locus_tag="OQN02_000205"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1641145.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(40009..40938)
                     /gene="fabD"
                     /locus_tag="OQN02_000206"
     CDS             complement(40009..40938)
                     /gene="fabD"
                     /locus_tag="OQN02_000206"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1641146.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40954..41907)
                     /gene="fabH"
                     /locus_tag="OQN02_000207"
     CDS             complement(40954..41907)
                     /gene="fabH"
                     /locus_tag="OQN02_000207"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1641147.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41975..43045)
                     /gene="plsX"
                     /locus_tag="OQN02_000208"
     CDS             complement(41975..43045)
                     /gene="plsX"
                     /locus_tag="OQN02_000208"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1641148.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43126..43299)
                     /gene="rpmF"
                     /locus_tag="OQN02_000209"
     CDS             complement(43126..43299)
                     /gene="rpmF"
                     /locus_tag="OQN02_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1641149.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43351..43872)
                     /gene="yceD"
                     /locus_tag="OQN02_000210"
     CDS             complement(43351..43872)
                     /gene="yceD"
                     /locus_tag="OQN02_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1641150.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            44071..44655
                     /gene="yceF"
                     /locus_tag="OQN02_000211"
     CDS             44071..44655
                     /gene="yceF"
                     /locus_tag="OQN02_000211"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1641151.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44767..45726)
                     /gene="rluC"
                     /locus_tag="OQN02_000212"
     CDS             complement(44767..45726)
                     /gene="rluC"
                     /locus_tag="OQN02_000212"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1641152.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46299..49484
                     /gene="rne"
                     /locus_tag="OQN02_000213"
     CDS             46299..49484
                     /gene="rne"
                     /locus_tag="OQN02_000213"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1641153.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49680..50633)
                     /gene="flgL"
                     /locus_tag="OQN02_000214"
     CDS             complement(49680..50633)
                     /gene="flgL"
                     /locus_tag="OQN02_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS1641154.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50645..52287)
                     /gene="flgK"
                     /locus_tag="OQN02_000215"
                     /pseudo
     CDS             complement(50645..52287)
                     /gene="flgK"
                     /locus_tag="OQN02_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52353..53294)
                     /gene="flgJ"
                     /locus_tag="OQN02_000216"
     CDS             complement(52353..53294)
                     /gene="flgJ"
                     /locus_tag="OQN02_000216"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS1641155.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53294..54391)
                     /gene="flgI"
                     /locus_tag="OQN02_000217"
     CDS             complement(53294..54391)
                     /gene="flgI"
                     /locus_tag="OQN02_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1641156.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54403..55101)
                     /gene="flgH"
                     /locus_tag="OQN02_000218"
     CDS             complement(54403..55101)
                     /gene="flgH"
                     /locus_tag="OQN02_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1641157.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55154..55936)
                     /gene="flgG"
                     /locus_tag="OQN02_000219"
     CDS             complement(55154..55936)
                     /gene="flgG"
                     /locus_tag="OQN02_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCS1641158.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(56074..56825)
                     /gene="flgF"
                     /locus_tag="OQN02_000220"
                     /pseudo
     CDS             complement(56074..56825)
                     /gene="flgF"
                     /locus_tag="OQN02_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(56845..58053)
                     /gene="flgE"
                     /locus_tag="OQN02_000221"
     CDS             complement(56845..58053)
                     /gene="flgE"
                     /locus_tag="OQN02_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1641159.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(58078..58773)
                     /gene="flgD"
                     /locus_tag="OQN02_000222"
     CDS             complement(58078..58773)
                     /gene="flgD"
                     /locus_tag="OQN02_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1641160.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(58785..59188)
                     /gene="flgC"
                     /locus_tag="OQN02_000223"
                     /pseudo
     CDS             complement(58785..59188)
                     /gene="flgC"
                     /locus_tag="OQN02_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59192..59608)
                     /gene="flgB"
                     /locus_tag="OQN02_000224"
     CDS             complement(59192..59608)
                     /gene="flgB"
                     /locus_tag="OQN02_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1641161.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59763..60422
                     /gene="flgA"
                     /locus_tag="OQN02_000225"
     CDS             59763..60422
                     /gene="flgA"
                     /locus_tag="OQN02_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1641162.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60498..60791
                     /gene="flgM"
                     /locus_tag="OQN02_000226"
     CDS             60498..60791
                     /gene="flgM"
                     /locus_tag="OQN02_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1641163.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60796..61212
                     /gene="flgN"
                     /locus_tag="OQN02_000227"
     CDS             60796..61212
                     /gene="flgN"
                     /locus_tag="OQN02_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1641164.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61252..62787)
                     /gene="murJ"
                     /locus_tag="OQN02_000228"
     CDS             complement(61252..62787)
                     /gene="murJ"
                     /locus_tag="OQN02_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1641165.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62954..63820)
                     /locus_tag="OQN02_000229"
     CDS             complement(62954..63820)
                     /locus_tag="OQN02_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1641166.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63822..64469)
                     /locus_tag="OQN02_000230"
                     /pseudo
     CDS             complement(63822..64469)
                     /locus_tag="OQN02_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(64480..65064)
                     /gene="rimJ"
                     /locus_tag="OQN02_000231"
     CDS             complement(64480..65064)
                     /gene="rimJ"
                     /locus_tag="OQN02_000231"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1641167.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65300..66508
                     /gene="mdtH"
                     /locus_tag="OQN02_000232"
     CDS             65300..66508
                     /gene="mdtH"
                     /locus_tag="OQN02_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1641168.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66572..67219
                     /gene="grxB"
                     /locus_tag="OQN02_000233"
     CDS             66572..67219
                     /gene="grxB"
                     /locus_tag="OQN02_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1641169.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67353..67913
                     /locus_tag="OQN02_000234"
     CDS             67353..67913
                     /locus_tag="OQN02_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1641170.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            68019..69065
                     /gene="pyrC"
                     /locus_tag="OQN02_000235"
     CDS             68019..69065
                     /gene="pyrC"
                     /locus_tag="OQN02_000235"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1641171.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69139..69384
                     /gene="dinI"
                     /locus_tag="OQN02_000236"
     CDS             69139..69384
                     /gene="dinI"
                     /locus_tag="OQN02_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1641172.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69674..69928
                     /gene="bssS"
                     /locus_tag="OQN02_000237"
     CDS             69674..69928
                     /gene="bssS"
                     /locus_tag="OQN02_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1641173.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            70043..71161
                     /gene="solA"
                     /locus_tag="OQN02_000238"
     CDS             70043..71161
                     /gene="solA"
                     /locus_tag="OQN02_000238"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1641174.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71209..71322
                     /locus_tag="OQN02_000239"
     CDS             71209..71322
                     /locus_tag="OQN02_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCS1641175.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71583..72149
                     /locus_tag="OQN02_000240"
     CDS             71583..72149
                     /locus_tag="OQN02_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1641176.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72153..72728
                     /locus_tag="OQN02_000241"
     CDS             72153..72728
                     /locus_tag="OQN02_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1641177.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72770..73822)
                     /locus_tag="OQN02_000242"
     CDS             complement(72770..73822)
                     /locus_tag="OQN02_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1641178.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            74047..74967
                     /gene="lpxL"
                     /locus_tag="OQN02_000243"
     CDS             74047..74967
                     /gene="lpxL"
                     /locus_tag="OQN02_000243"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1641179.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75139..75336
                     /locus_tag="OQN02_000244"
     CDS             75139..75336
                     /locus_tag="OQN02_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1641180.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            75360..>75869
                     /locus_tag="OQN02_000245"
     CDS             75360..>75869
                     /locus_tag="OQN02_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1641181.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVS"
BASE COUNT        18495 a        20068 c        18764 g        18542 t
ORIGIN      
        1 atctgtcgtg caatagatct atttcttgca gttccattat tacaggctca agaagcgtta
       61 ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc attgtcgcca agtgtttcaa
      121 gccatatgcg caatatttcc agcatgtttt cactgtgaga aagtgcaaga aatgcgcggt
      181 ctattgtttc gtggtaaata tcacgcatat taatccccgt ccgtcgtttt tcttaaaata
      241 acctctgtca cgaaatcagc ataatcggcg gcgatattca gtacatcaag cccgttgatt
      301 tatattctct cgtggaaagt aagaaaaaag ccgctctaat aagttctggc attgacgaaa
      361 gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg tttcagggaa ttaaaacgat
      421 catcacgcat aacccccccc attttcacaa tcggcaacaa gaatattttt agcgtcattc
      481 agtgaccgcg ttgcgatgta tcggatacat tccagggcga actgtgtccg gtctttctgt
      541 tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa taagcgcgtg catcagcgta
      601 gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt gcataggttt ccccctgacg
      661 aatacgggcg gcgaatacag caacacaacc ggacgggcaa cggctacgcg cttcgcgttc
      721 cgtccaagcg gttacgtgga tgatttggga ttctccggca ctcagtgcca gaaaacgcca
      781 cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga tttacggcaa ccataacggc
      841 tcctagttta cgttgttggt tagaagcccc gttactgctc ctacagtgcg gggctttgct
      901 ttttgtgttg attgtgtgta atgtgtcact acacataaat acattacctg aggtgtaatt
      961 aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc cgttccagtt ccgtttagat
     1021 cctgacttac gggaaaagat ggaaaaggca caacaacagg acggcgatga ttctcttgct
     1081 gcctggataa agcgaataat ccgcaaagaa ttacagcaac gcggcataga acccaaagga
     1141 taaaaactat cagcgccgtg gtgtgaggta ctacggcgca ttgctttaca ggcgcacaca
     1201 atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac gaaaaagacg acgcagaaag
     1261 aaagagcatg aatcagaacg attcgcgccg tgttcttttg cccttgagaa gtaccttaaa
     1321 gaatactcat caaaggagag aacttcgcag ttatggcaac gcactgaacc agactgattg
     1381 cattgcccac cagcctgata gcggctatca tccccgtgct tatgtttggg atcacacaca
     1441 caaacggcgc agcgggttat ctgttcagaa aggcggctcc atttcggggc cgcttttttt
     1501 atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac cagggcgaac gaatccccgc
     1561 ccacgttctg gcgtatattc aatcttcatg gttatagctc tgtgttcaga tgattgatgt
     1621 gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct tgcagctatc cttccaggcc
     1681 agaacctcgg ataaagacca gccaacggaa cgaccgccaa gtttacgacg tgatgggaat
     1741 tgtccggcct tttccaggcg gtagcggcat gagcggctaa ggcctgttag cttttcgcat
     1801 tctttttcac gtataaaccg atcagtgctt aacactattg ccccctttcg tttcttaaag
     1861 agttatttcg tgttctattg cgttgggatg tgtctgattg tgtcaggatg attcagagtt
     1921 ggcaaatgtt gaggccgcat ggtttacaga aagaggaata atcaggataa aatcctttaa
     1981 attcatgtta atacaaaggc ataaaatatt gtttcatgcc ttttttctcg ctatttaaag
     2041 agtgattcgc tagtgtataa aaaatcagca acacattaaa aatcagctat ttataaatct
     2101 gtacactttt tcacctcttg ttcgtagttg ttccgctttg ttgctcattg ttgcacgttg
     2161 tatctgttcg catatccagt atgcgcatac tgaaaaaaca cgaaaaaaat tattttcttc
     2221 tggctactgg tagcgtggtt acgttttcat gtgttcccgc cagtatccct aaccgctccg
     2281 tccacatatc cagcgcattg cgtttagcat ccagataacg ggaatgatta taaactcgct
     2341 gcattcctgg catctggtgg cctgtaagct gctccacgac gtgaggatcc acgcctaaat
     2401 cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag tgaccagtga gggtgattta
     2461 gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt ttcctgtttc acttccccca
     2521 gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt gacgaacggc agtattgctt
     2581 ccggtatggg ccggaatatt gccaccttcg ttttgctgtg ttctttcggc acggtccaga
     2641 gcatttcggt aaaatcccac tcgctgatct ccgataacct cagttctacc gtccggcatc
     2701 cgaacacaat caggaggcgg attaacgcga tgtagtaggg ggagaatatt tttttgtcca
     2761 gtgcctgcaa taattcgccc agttctttgg tgcttaagac acgctcgctt atatccggtt
     2821 ttttcccaac gtccgccaca ctcatatcat caagaacgtt gctgattgca tagcgccgcc
     2881 tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa gaatccggca gtaacaggcg
     2941 ttcgctttgc cacctggtca aaacaggcca gccagtgccg tagctcgcat ttatccagcg
     3001 gcatagcacc aatgtgctgt attacgtgat tattaaggcg ctttttcagg gcggcataat
     3061 ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt gagcgcatcg ccaaccgtta
     3121 ccggctttaa cgcttcctgt acggtgtaat taagctcatg acgtggattt tcccctctgc
     3181 cagccatgcg cgacactggg cggctttttc cctggctgat ttcaggttca gatcaggata
     3241 atttcccagc ttaatgcgtt ccggtggtgc cccccttccc gttccggccc tgtaagtgaa
     3301 ataccaggtt aaaaggccac tggttgaatg cctgacgctc aggtttccac cgtcattaag
     3361 aaaggctgtt ttttgggcgg gtgtgccgtt gattttcctc agctgtgtat cgctcagttt
     3421 gttaagtgct ctgctcataa ttttgattcc ggtcatcaca attaactaca ccattaacta
     3481 caccgatcgt tgcacaaagg gctacaacgt gaaacaagct ggaacaagga aaatcacaaa
     3541 tctgtttata atcaaaaaca taatgaacaa tctgaaacat tatgaaacgg caaaaaacac
     3601 taattgataa aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg
     3661 cagataccag cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt
     3721 ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta
     3781 ttgacccgat ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc
     3841 gactggtcag tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg
     3901 atcatctcgg tgaaaccaac tggtcattac tggtacaggc agtaaaccac tggcatgagc
     3961 cgaccgccgc gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga
     4021 tgatttcttt ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt
     4081 ttatcattca gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac
     4141 agcactgccc acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag
     4201 agctggagcc tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc
     4261 tggaaaatgc gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta
     4321 gcggatttgc cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg
     4381 atgttccacc tcgtgctcat cctgcggatg ttctgccgca agagatggat aaactgcgtg
     4441 agatgatgct cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta
     4501 tatcccagtc acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg
     4561 aaatctacga tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat
     4621 tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg
     4681 cactggatgc actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg
     4741 aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg
     4801 aagggtaagt ttggatttaa gccggatgca gcatcacatg ccgcatccct tttcgcttac
     4861 ttccgttgcg ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct
     4921 tccagaatca caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg
     4981 caaggtaatc ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt
     5041 tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc
     5101 tcaaccactt tctcgcgcat attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa
     5161 tcaggatgta accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct
     5221 tcaaactgtt tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg
     5281 cctttcatgc tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca
     5341 tccgccggaa cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct
     5401 ttcgccgtgc ccggatgaac attgttaccg acaattttta tattgaccga tgcggcgttg
     5461 aagttttcaa actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca
     5521 tcgaaggcat caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag
     5581 gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg
     5641 atttctgcaa tacctgcttt gtcatcagca cctaacaagg ttttaccatc ggtggtaatc
     5701 aacgtctgac ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg
     5761 ccgataccca gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt
     5821 ttgccgctgc aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg
     5881 ccagggacgt tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg
     5941 ataagcccca tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc
     6001 gtgctgggaa cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa
     6061 aatcgctcaa gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga
     6121 agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat
     6181 cagcattacc ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc
     6241 aagcggtttg ccgtagaatt accgccctca ttaagagtca ccaaggtggt taaccacaaa
     6301 ccccgcatcg gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac
     6361 aaccgagttc gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta
     6421 aagaggtcat tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg
     6481 gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg
     6541 attccggacg catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct
     6601 atgtgaacac tgttttccaa agctatgcac ttttccccca catgaccgtg ttcgaaaatg
     6661 tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg
     6721 aagccctgcg gatggtgcag ctggaaacct tcgctcaacg taaaccgcat cagctctctg
     6781 gtggtcaaca gcaacgcgtc gccattgctc gcgcggtggt taacaagcct cgtctgttgt
     6841 tgctggatga gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc
     6901 tgaaagcgtt acagcgtaag cttggcatta cattcgtttt cgtgactcac gaccaggaag
     6961 aagcactcac catgtcggac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg
     7021 gcacgccgcg tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg
     7081 aaattaatat gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca
     7141 acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac
     7201 tgcatgttct gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg
     7261 ctgaagggct gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg
     7321 ttgttgaact ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgatc
     7381 ctgactttga ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg
     7441 tcgtactggc tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc
     7501 ggttggcttg tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg
     7561 acccgcgacg acgccagttt cgtcaaaatg gtctttacgc tggataacta cacgcgtctg
     7621 ctcgatccgc tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc
     7681 ctcgcctgcc tggtgctggg ctatccattt gcctggtttc tggcgaagct gccacacaag
     7741 gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct ggaccaactc attgattcgt
     7801 atctacgggc tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg
     7861 ctgggcgtta tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt
     7921 ctggtttaca ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg
     7981 gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc
     8041 cgcatcatta ttccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg
     8101 ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc
     8161 ggtaacgtca tcaaggtaca gttcctcaat attcgtgact ggccgtttgg tgcagccacc
     8221 agcattacgc tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt
     8281 ttgctgaata agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga
     8341 ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca
     8401 gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga
     8461 acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga
     8521 cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgcg
     8581 gtaagccgtt cgttagcgga atgctgtttg tggtaatgat gtcaccagat atcgtgatgg
     8641 caatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc
     8701 tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga
     8761 aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc gaatttacca
     8821 ttctgcgaaa aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa
     8881 gctttaccct gtcgatggac gatgtggtag tttcttcatt cgtcaccgga ccaagttatg
     8941 aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg
     9001 cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg
     9061 ctcgtgataa aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct
     9121 gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac
     9181 gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa
     9241 agaaaccggt attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa
     9301 gctgaaaacg tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga
     9361 taaaatgcgt aaagagggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag
     9421 caatctcgat ccagacatgc tcaacaagcc ttttgacccg aataacgact actccattcc
     9481 gtatatctgg ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt
     9541 caccagctgg gccgatctgt ggaagccaga gtacaaaggc agcctgctgt tgaccgacga
     9601 tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga
     9661 tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc
     9721 gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat
     9781 ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc
     9841 gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa
     9901 agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc
     9961 tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt
    10021 ggcaaacgat aagacgctct acccggatgc tgaaaccatt aaaaatggcg aatggcagaa
    10081 tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg
    10141 ttaattcagc aagcgggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct
    10201 aatgagtcag gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta
    10261 ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct ccagcaactt
    10321 ttcaacaaat tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt
    10381 accaacctga ctcggttcaa gattcagttc aacggtgtga gcaccatgta gtttggcttc
    10441 gtgtacaaac ccagccgccg gatagacatg cccggaagta ccaatggcaa taaagacatc
    10501 cgccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac
    10561 gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac
    10621 gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc
    10681 atgcatatga atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt
    10741 taccagcaaa aagcgatcgc cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc
    10801 gttaggctga atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac
    10861 cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc
    10921 ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc
    10981 tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa
    11041 aatacgctga cgcaaacgct cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg
    11101 atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc
    11161 cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc
    11221 tacaggtaag agatgacaag gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt
    11281 cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag
    11341 agaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg
    11401 atcataaagc gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag
    11461 ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca
    11521 gcggcgtaac gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg
    11581 gccaaactcg ccggtaatgt agcttttacc agtaatcggt ttgccgttga aaaccagccc
    11641 gccgccaacg ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc
    11701 atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc
    11761 aagacgtgcg ctcaggtcgg cacgcagcgg tttgccgctg gcagcgggga cattagcggc
    11821 atacagctta ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt
    11881 acagccaaaa cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc
    11941 atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa
    12001 cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg
    12061 ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc
    12121 gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga
    12181 agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaataaata tcgctggaga
    12241 ggaactgatg accgatcagc ttttcaatcc actcaataat cggggtaagt tgcagtgaaa
    12301 caactacgcc gataaccacc ccacacaggc tgccgaacag ccctgccagc aatccatacc
    12361 agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat
    12421 cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc
    12481 caatcaccag taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca
    12541 tatagccgta agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg
    12601 catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca
    12661 cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt
    12721 gatcgagttg accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct
    12781 gcatcagttt atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct
    12841 tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa
    12901 aattgcgcca cgcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt
    12961 gtgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc accagaccgg
    13021 tgaaattgat atacggcgca gcggcggcaa tacccggcac tttctgcacg ttatccagtg
    13081 cttcctgcca gttagtccat ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg
    13141 ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca
    13201 ataccgccac gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc
    13261 cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca
    13321 ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg
    13381 gcggctcata cgtttcgcca gttgcaggtc gtgagtgacc accaggaaag cggtgccctg
    13441 caagcgattc aactccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt
    13501 accggtaggt tcatccgcca gtaccagacg cggattattg accagcgcac gggcaatcgc
    13561 tacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag
    13621 ccccaccgct tttaacatct ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc
    13681 aatcagcagt ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa
    13741 ctgataaata aagcccagct tctggttgcg cagttctgct ttcgccgctg aagacagttt
    13801 gctcatcggc tgaccgttaa agatcacatc gccggaggtt ggcgtatcca gcccgcctaa
    13861 caggtgcagc aaggtacttt taccggaacc agagctaccg acaattgcca tcatttcacc
    13921 ttcgcccacg ctgaaactga cattgagcag cacatcggtt tgcacactgc cttcctgata
    13981 gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg
    14041 gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg
    14101 ccaccagcgc aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat
    14161 caagcaggac gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa
    14221 gcgccgctcc gaggattgca ccgataatcc cggcgctagc cccttgcacc ataaagacca
    14281 tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct
    14341 ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc
    14401 tcagcagtaa gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac
    14461 ggtcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa
    14521 ctctcagcgg ctcatccagc cacaaacgcc agccggtaat attggctgcc ggataacgca
    14581 tcagacgcga tgcatcctca atattcacca gcatttcata gccatcgact tcactgttgg
    14641 cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga
    14701 actggctggc agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg
    14761 aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca
    14821 cattgaccag atacggcgtt agtggatctt tttgcgccgg gtcgataccg agcatcaccc
    14881 cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc
    14941 gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat
    15001 gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct
    15061 caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga
    15121 tggtagaaag ccaggagacg aaacgaccga agcgatccac tgcacgccca cgcatgtaac
    15181 gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa
    15241 gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag
    15301 ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatgtttt atcatcaatc
    15361 ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg
    15421 ggtcataacc agcaggatca accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa
    15481 acaaaaagag ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta
    15541 tcactcggtc attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt
    15601 cctgagcaaa tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt
    15661 tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg
    15721 tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg
    15781 gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc
    15841 caatgccgct tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac
    15901 cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg
    15961 tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc
    16021 cacgccgtgg tggggaatga acagtgtgat acgcaatttg ccttattaca gccttggcgc
    16081 atggtttggc gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct
    16141 gatggcttct ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct
    16201 gttgtcgctg gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt
    16261 cggtatgcgc tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac
    16321 cacccatcgc attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc
    16381 acgctggtcg ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt
    16441 catctgtact gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct
    16501 gttgttctcc ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc
    16561 agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta
    16621 tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc
    16681 ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt
    16741 taaccggcgc agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg
    16801 tggtactcat tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt
    16861 tcaccgatca gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt
    16921 cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc
    16981 agcgtggcat actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt
    17041 ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac
    17101 gaacccaact ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat
    17161 acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc
    17221 ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca
    17281 cgctggagga agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag
    17341 cggcaattga actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg
    17401 aacatattta ccagcaagtg agtaaaggca cattacctgc tgggatcgag tactggcagc
    17461 cattgttctt cagcgaacca ctgccgccgc tgttcagtta tttccctgcc aataccttgc
    17521 tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt ccaagctgac acgctggcgc
    17581 gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc
    17641 tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgagtgcag ctaaaaactg
    17701 aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg
    17761 ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg
    17821 acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc
    17881 tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg
    17941 ggcgttatct gatgattggc gctgccgaac atggttttgt cgataaggcg cgtaatctgg
    18001 cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc
    18061 gccgcaccat caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc
    18121 cggtggtcca tctggagcac ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg
    18181 gcggcattac tggcgagtac ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc
    18241 cggtgtcgtc actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc
    18301 tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc
    18361 gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg
    18421 cgtttaaaca cgatcgtgag cagtatcagt tattctgcga cagcttcccg tttgaaacca
    18481 cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa
    18541 tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg
    18601 ctttcctggc agtagataac cacaagcagg tagcggtgtt ggtgcctacc acccttcttg
    18661 cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa
    18721 tgatctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag
    18781 ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag
    18841 atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca
    18901 ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta
    18961 cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc
    19021 gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga
    19081 tcctgcgtga aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca
    19141 ttcagaaagc cgccgaacgg ctggcagaac tggtgcctga agcacggatt gccatcggtc
    19201 acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt
    19261 tcaacgtgct ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca
    19321 ccattatcat tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc
    19381 gcgtcggacg ttcacatcat caggcatatg catggctgct gacgccgcat ccaaaagcga
    19441 tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag
    19501 gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag
    19561 aacaaagcgg ttcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg
    19621 ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa
    19681 cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca
    19741 cgcgtctgtc gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga
    19801 tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg
    19861 atattgcccg tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg
    19921 agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg
    19981 gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta
    20041 ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac
    20101 tggaagagaa cgcgatcgct taatgtaaat cgggggcgtt tctgcgcccc catctgcaac
    20161 atttacaaat tctttgcact ttcctgcact atccgacact gtcaccatcc ataattcagg
    20221 cttatctgtt tattacaata accttatatt tattatgtat ttttggtgat gatcaaaacg
    20281 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg
    20341 gcaaaagcca acacctggcc gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa
    20401 tttcatgtgg tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc
    20461 ggctttctcg ctctgttaca agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc
    20521 agcgtgttaa cgatcccgtt gcaaacccta cttccagacg cgccgcgcga aggcattgtg
    20581 atcaacattg cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg
    20641 tatccaatag gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc
    20701 gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat
    20761 aaagcacagg gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc
    20821 catcatgcga ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc
    20881 gatttcggca ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc
    20941 aaaacactct ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata
    21001 aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag
    21061 aagattgatg acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt
    21121 aaagatgcag cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg
    21181 atgccggtgg atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa
    21241 aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga
    21301 gggttaatgc ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag
    21361 aagtaatacg gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca
    21421 gcgatcccag atttgtcccc gcgttagcac tgatagtacc gactttttgt tggccttctg
    21481 gcgttgactg aacttcgaca gccgcaaagt tggcaaatga catggagctt aaaatcgccg
    21541 cagcgatgag gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca
    21601 agaaattact taacgatcga taactaaatg atagatgtga tctggatcac atacaagata
    21661 ttttttataa caatcattaa ttaatttaaa aatctttaaa tttcagtcaa atagaaagca
    21721 attattttta tgggatctgg actggtgaac ggaggtgatt atttttataa taacctttca
    21781 ttcacagtca ccaggtacaa cggcatcatg gcaactgact caacacaatg tgtaaaaaaa
    21841 agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc ttgataaggc catgaaattg
    21901 ttctggcaac acggttatga agcgacttct cttgcggacc tcgtcgaagc aaccggagcc
    21961 aaagcgccca cgctatacgc ggaatttacc aacaaagagg ggttatttcg tgccgttctc
    22021 gaccgctata tcgatcgttt tgccgctaag catgaagcac agctgttttg tgaagagaaa
    22081 agcgtggagt ctgcgctggc tgactatttt gcggccatcg ccaactgctt taccagcaaa
    22141 gacaccccgg caggctgctt catgatcaac aactgcacca ccctctcccc agattcagga
    22201 gatatagcca atacgttgaa atcacgccat gcgatgcaag agcgcacttt gcagcagttt
    22261 ttatgtcaac gacaagcgcg cggggaaatc ccgacccact gtgacgtgac acatctggca
    22321 gaattcctta attgtattat tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg
    22381 gaaaaactga tgcagattgc cggaacgact ttgcgtttat ggcccgaact ggtgaaataa
    22441 gtcgcaaaaa aggcccctca gcctgagcga atgaggggcc aaattgcaga gtacagcctt
    22501 gttatacttt gttattcaaa atcagttgcc cgttactatc tagcgggatc tgcgtacccg
    22561 gatcgcggtc catgcggatt ttgccctgct gatcgccaat cttataggtc acatcataac
    22621 cgagcatttt ttctgacttg tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag
    22681 tatcgctttc ctggagagag ccctggatct ggttaccggc atatccacca cccagcgccc
    22741 ccacaacagt ggcgacatct ttaccgcgac caccaccaaa ctgatgccct atcacgccac
    22801 cagcaacagc gccgagcacc gacccggtaa tgcgattttc atcctgcacc ggtcgacgat
    22861 gggtcactgt gacgttgcga cactcctgac gcggtgtttt aaccgtttcc ttgattgggg
    22921 ttgcagaaac aacctgagcg tattgcgggc cccgttcaaa cacgttcaga ctggccactg
    22981 ccgctacgcc caacgcagct gcgacaccaa tcccgatacc cgccaacatt gatttattca
    23041 cgggaacacc tccttctttg cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta
    23101 tcagacagaa gatgaaaagc gcgggaatga ctggaaatac ttaaggattc ggaggagtct
    23161 gaaggaatgc ctgatgcgac gcttaacgcg tcttatcagg cctaccgctc gtaccatttg
    23221 tccagggcgg ataaggcgtt tataccacat ccgccagtgt acgtcgatta atgcaacttc
    23281 aaacgcggac ggataacgcg gttaatactc cccaccagca tcattaatcc ggttttaaag
    23341 taaccatgca gcgcaatctg atgcattcgg tatagcgaga tatatacaaa gcgcgcaatt
    23401 cgtccttcaa tcatcattga gccgcgcgtc aggttaccca tcaggctacc aacggtggag
    23461 aagttcgaca gcgataccag cgaaccatga tctttatact gataattttt cagcggctta
    23521 ccgttcatct gcgccagaat gttgttcatt gcgcaagtcg ccatctggtg tgcagcctga
    23581 gcacgcggcg gaacaaagcc cccttccgga cgcgggcatg acgcgcagtc gccaatagcg
    23641 taaatgtctg gatcgcgagt ggtttgcagc gtcggttcca ccaccagctg gttgatacgg
    23701 ttagtttcaa gaccaccgat atctttcagg aagtctggcg ctttgatccc ggctgcccat
    23761 accatcagat cagcctcaat atattcgcca tctttagtgt gcaggccgcc ttcatcagca
    23821 ctggtgacca tggtttgcgt cagcacgcga acgccaagtt tcgttagctc gttgtgggcc
    23881 gcagtagaga tacgtggcgg taatgcaggc aaaatacgtt ctcccgcttc taccagcgtt
    23941 acgttcaggg cttcgttggt caggcctttg taaccgtagc tgtgcagttg cttgatcgcg
    24001 ttgtgcaatt cagcggagag ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc
    24061 actttgccat ttgcgcccag gttggcagag tatttcagga acagattcag catctcctgg
    24121 tggaagcgac gcgcctggtg cgggttatcg aggaaaatgc agttctcttt gacgcctggc
    24181 gtattgaaat cgttagaggt gctacccagc gccatgacca gggtgtcata ggcgatttta
    24241 cgttccggaa ccaacagttc acctttctcg tcgcgtagtt ctgcaatagt gattgttttc
    24301 gcttcacgat caatatcaat gacggaaccc aactggaact ggaaaccatg attgcgcgca
    24361 tgggccagat agctcaacgc atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc
    24421 agcagtggtt tccacaggtg gctgtggtta cgatcgacca gcgtaatttt ggcttttttc
    24481 ttacgtccca gcttatgccc cagctgtgtt gccatttcca gcccaccagc accgccgccg
    24541 acaatcacaa tctttttcaa tggcgtagtc aacgtgaccc ccttaaattt attaaccaat
    24601 tgttaattaa tggttattaa catagctctt aattaacaac aggttacagc aatgaaaatg
    24661 ttcttcgcta ttgagaataa catgaatggt gcattggtca taccaaaatt gatgttaatc
    24721 aagttttgtt gcttaaaaga taggtaactt taccggggga gatcaaaaaa tagagtttat
    24781 tgtttattat ttgagcaaat tcggaagggt aatatagggt gaagcgtata tcgagggcgt
    24841 ttggtcgtcc tgataagatg cgcagcatcg catcaggcat tgatgctcga ctgtcggatg
    24901 cggcgtggac gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta
    24961 cctgatgcga cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc tgtaaatgcg
    25021 gggagatatt cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat
    25081 gcaactcttc agatgtccgc tggctgttaa gtgcttcatc attacgcgac aaaataacca
    25141 gacaacgatc gcgattcttc tcgcggaagt tggtcacaca cttagtggca atatcggcat
    25201 actcttccgg gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga
    25261 agatcacctg gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccaggc
    25321 caacgccgca aattagcgga cgctcgtcaa cgttcagttg caacattttg tccacttctt
    25381 taagcagatg ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat
    25441 ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa
    25501 aaccgtgtaa atagataatc atcgcttcct cacataagcc agggttagtg acctgctttc
    25561 tcttcctgcc agcgttcatg taactgattc agacgggtgc tgatcgcttt ccagcgagcc
    25621 gagtccatca gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct
    25681 gccttgatcg gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc
    25741 aggatcatat cgcaacccgc atccagtgat gcctgcccgc gttcggcata actgcccata
    25801 atcgcggcac cttccatcga taaatcgtca gagaaaatca cgccgtcaaa acccagttcc
    25861 tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg ccggacgcgg atcaacatca
    25921 ctgtagatca catgcgcagg cataatggcg tcgagtttat tttcgcggat taaggaactg
    25981 aagaccgaca tatctttagc gcgaatctcc gcttgtggac gtggatcgca cggtgtttct
    26041 ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt
    26101 ccggcttcat gcataccatc aataaaccgg ctggcaattg ccagggcttt ttgtggatcg
    26161 gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc cgacatccag cactggcgca
    26221 aagctgatat caatatccat agcgatcatt tcgctggcca tcagccaacc cgcctcttgc
    26281 gccagtttgc cgccctcttc cattcccaac agcgcagcaa aggattgtgc tgccggtaag
    26341 cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt cctgatctac cgccaccacc
    26401 agatgattgc gcgatgctgc gcggatctgg cgcaccagtt cacgtaactg ggcaggatca
    26461 tgatagttac gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc
    26521 tcttccgcgt ccagttcgta accttcgaca tccaacatta ctggacccac actgctctcc
    26581 ttattgtttt attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc
    26641 gccggtttgt cgccagcggt actcaaacca ccctcctttg agcatcagca gccagggaaa
    26701 ccatcgcctg acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc
    26761 attgaccaat tgccggtgct gttcagtatt ttccacccac accgccgcca gttccagcgc
    26821 gatatcacca tctccagcat actcccagtc gatgagtttt aaccctgatg cgctatgcac
    26881 taaatttccg gcgtggacat ccatatgcaa tggacttaag cgtaaaggtc gtggttcccg
    26941 cgctttgcgc agacgtttta acattcgcag ccaacccaca gtccgccgcg ccggatcgct
    27001 ttgctgccag tacagttcca gtaagggcaa cagcgttatt cgccagccaa aacgtggttg
    27061 ttgatgtaga taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac
    27121 cgcgccgggc agatagtcga ctaccatcca gtcacggaga tataaatgcg gcttcggtgc
    27181 aatgcttgcg ggtagttgtg ataaagcccg atactggcgc aagaacgcgg actgcggcgc
    27241 atcaggatcg tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact
    27301 accgccacta agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga
    27361 cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta cctgaccaga
    27421 taatttcgcc cgtctgcacc agcatcagct gcatttgtag ggtcggagcg ttaacgttgc
    27481 cagaggcgct ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt
    27541 tactacgggt gcctaaactg tcctgcggcg acaaacctaa ctgttgctta gccatcgaca
    27601 gttgctgggc ggaaaccagg gtaaatttcc cgttattagc cagcgcattt cgcagcgttt
    27661 cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca
    27721 ggacgctacc cgcagtcacc ccgtcagccc caagcatctt actgaccatc ggctgcattg
    27781 cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg tgccgttcga tcttcgtgct
    27841 caattgggcc tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt
    27901 gtggctcggc tggttgttcc ggcgctggtt tcacttcttc taccggtgca ggttcacgtt
    27961 gccccacaca cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt
    28021 ttgtcatcaa gattcacccc tttacaaata tagataaagt ctgactttgt gcgcccccag
    28081 aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa cgctgcgtgg
    28141 cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac gataatgaac
    28201 ggtaacgggt tcttgccttt cgttataaag cgttgaggat gctgaaggtt gaatatcaga
    28261 cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg actccatcac
    28321 cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca ccagcagtaa
    28381 caacaccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc tttatgcgcc
    28441 agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta aacctcttgt
    28501 ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat accttcttgc
    28561 tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac
    28621 acgtcgttca cagtcggaat gagaatattc ggaatgatca gaatatgcgt tggcgcttgc
    28681 ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc ggaggggatc
    28741 tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc ctttttcaaa
    28801 tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt atggtcgagt
    28861 taaacctgct ctttcaatct taactcacga tttcttaagc aaaaaatcgt tttgctgccg
    28921 tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt
    28981 ggtttacatg acaaatatga atgcgtatat ttctcatttg catttgcatt tgcagcaaaa
    29041 ttattcgcac actaaatata accgctcatc accctcctcc ggcatgagcc tgacgacaac
    29101 ataaaccaag agatttcaaa tgctttcaac acaatttaac agggataatc aatatcaagt
    29161 catcaccaaa ccgtcactac ttgccggttg catagcactg gcactattac cttctgccgc
    29221 ttttgctgca ccagccactg aagaaacggt gattgttgag ggttcagcca cagctccaga
    29281 tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac
    29341 tcaacgtgat attcctcagt cggtcactat tgttagccag cagcggatgg aagatcagca
    29401 gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga
    29461 ttccgatcgt gctctttatt attcccgcgg attccagatc gataactata tggttgatgg
    29521 tatccccacc tattttgaat cgcgctggaa tctgggcgac gcactttctg atatggcact
    29581 gtttgaacgc gtagaagtag tgcgtggcgc gacaggactc atgaccggga cgggtaatcc
    29641 atctgcggca attaatatgg ttcgaaaaca cgcgacctgt cgtgaattta aaggcgatgt
    29701 ctcggcggaa tacggtagct ggaacaaaga acggtatgtg gcggatttac aaagcccact
    29761 caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc taccagaata acgactcatg
    29821 gctggaccgc tacaatagtg aaaagacctt cttctcgggc attgtcgatg ctgatttagg
    29881 cgatcttacg acgctttcag ccggttacga atatcagcgc attgatgtta atagcccaac
    29941 ctggggcggt ttaccgcgct ggaatactga tggcagcagc aacagttacg atcgcgcacg
    30001 cagtaccgca cctgactggg cgtacaacga taaagagatc aacaaggtct ttatgaccct
    30061 gaagcagcgg tttgctgata cctggcaagc gacactgaat gccacccact ctgaagtcga
    30121 atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt
    30181 tgggccatac agtaattatg gacctggctt tgatcatgtc ggcggcaccg gttggaacag
    30241 tggcaaacgt aaagttgatg cgctggattt gttcgctgac ggtagttatg aattgtttgg
    30301 tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa caaaacaatc gttcagttca
    30361 tgggccaaca tcttcccgga tgaaattggc agtttctaca actttaatgg caatttccca
    30421 caagccgact ggtcatcaca gagcctggcg caggacgata ccacacatat gaaatcgtta
    30481 tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat
    30541 accaactggc gggttgatac gctgacttac agcatggaga aaaaccacac cacgccttac
    30601 gctggtctgg tgtttgacat caatgacaac tggtcgacct acgccagcta tacctctatt
    30661 ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tggctccaat caccggtaac
    30721 aactacgagc tgggtctgaa atcggactgg atgaatagcc gtctgaccac cacgttagcc
    30781 atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat ccccggcagc
    30841 aacggcgaaa ccgcctataa agcggtggat gggacagtca gtaaaggggt ggaatttgaa
    30901 ctcaacggcg caattaccga caactggcag ctgacatttg gcgcaacgcg ctatcttgca
    30961 gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacggt taaaatgttc
    31021 accagctatc ggttgcctgt catgccagag ttgacagtcg gcggtggtgt taactggcaa
    31081 aatcgcgtgt ataccgacac cgtgacaccg tatggcacct tccgcgccga gcaaggtagc
    31141 tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc gttacagggg
    31201 aacgtcaata acctgttcga caaaacctac gataccaaca tggaaggttc tatcgtctac
    31261 ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac acctgtaaaa
    31321 aaggcagcca tctggctgcc ttagtctccc caacgtctta cggattagtg gttacggatg
    31381 tactcatcca tctcggtttt caggttatcg gatttagtac cgaaaatcgc ctgaacacca
    31441 gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg atccacttta
    31501 gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt
    31561 tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc tgttgctttt
    31621 gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt aatcagcacg
    31681 cggaagatgg tgtagtaaac aatcgcataa ccgataccga cgatcggtaa cagccacagt
    31741 ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg
    31801 tcacgcatcc ccagaagaat acagattggg aatgccaggc ctgccagaat cgcgtggatg
    31861 atgtacagga tcggcgcaac gaacatgaag aagaactcga tcggctcggt gataccggtc
    31921 aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcacg gttttctggt
    31981 ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt gaacaggaag
    32041 ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat gtcgccgtgg
    32101 aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg tacgttccag
    32161 atgtggtgca gaccaaacgg taccaggcaa cgttcgatga aaccgtaaat gccaaacgca
    32221 actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga accaatcggc
    32281 ggccaaatga aggacagcac aacgccagta aagatggcag ccaggccaga aatgatcggc
    32341 acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg gtagaaacgg
    32401 ttaaacatgt acgctgcaat cgcaccggag ataatccctc cgagtacgcc agtatccgcc
    32461 aagtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc
    32521 atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac gccatcgtta
    32581 ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc aaagacggaa
    32641 ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa attcgcggaa
    32701 ccgacgccca gcagaatacc tgcgataggt agtacggata ccggcagcat cagcgattta
    32761 ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc tcctgagtat
    32821 gggtgctttt tttacgttct cacgcgtggc aaggggggag agcctcgccg tgtacagggc
    32881 atctaagcgc cctttattta ttacacagag taaaataatt cagtgccaat ttgtttgacg
    32941 gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc
    33001 acaatatttc aaacgcggtt attcaccgcc caattttcgg cggtgaactt tactcgtttt
    33061 agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagcg
    33121 tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca
    33181 acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct
    33241 tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc
    33301 agggggacat aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt cacaatgccg
    33361 gaaaaggaga tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta
    33421 aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta
    33481 tcggcacggg cgtcacgggt atggacgata accggcttgt tcagttcacg accaatctgg
    33541 atatgatgga tgaaggactc ttgctgacgt actttagttt ccggtgtgta ataataatcc
    33601 agcccggttt cacccagcgc tacaacaccc tcttctgccg ccagacggcg taaatcttct
    33661 acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtca
    33721 cgttcgccta ccagatcccg catatgtaaa taacccggta atgttgtggc gactgccaga
    33781 caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa
    33841 gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc
    33901 ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa
    33961 aagatcggtg atgagaagct cgcggttgat gcctgtgaca gacattaact gttcacgaat
    34021 gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt ttgccagttc
    34081 ggcgaccagt cccggcacat caacattggt cacctgcgca gcgccatgat ggcgttttag
    34141 cgcatccatc agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt catgattaag
    34201 ggccgccagc agcgaatacc agtcgcccga tggcacgcta tatgccaacg cctgacacaa
    34261 tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgcgc caggcgaacc
    34321 ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac attgtcactt cgcgtgaaag
    34381 ccaagtcacg gcgtactgtt ctggaggcgg cgcaaggtaa tgtaaccgac aacgactacg
    34441 taatgttgcc agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg tttctgctgg
    34501 tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc
    34561 ggttacccag acaactttcg caccacctag gcgtgcgtgc tcattcagct tttcggtgac
    34621 ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt tcgggagcca gggtgtagta
    34681 atcgggatgc gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg
    34741 gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc
    34801 catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc
    34861 taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc
    34921 acccagtggg tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca
    34981 tcaatggtat gaatactttt atcttgtgca gccagttcca gatagcgggc gcgggtgcga
    35041 ttaaagaaat cgaaagactc ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt
    35101 tttaagccaa cttccggggt aacatcgaga tagagcgtta agtctgggcg aaaatccccg
    35161 agaacagcat cacgcagtgt tgccagcata tgttggtcaa taccacgtcc tccgccctga
    35221 tacgcctgag tggaaagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca
    35281 ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag aacttcggct
    35341 ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt
    35401 tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc
    35461 tcgagcgtct caaccaccac attacgcgcg gtagttttgc ctgcgccttc cagcccctca
    35521 atgacgatat acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga
    35581 cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa
    35641 atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccccggcgt
    35701 agctatcgca cccggcggca aaccggtaat ggtataggtg ttatacgctg tcggcgtttc
    35761 caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggttgg
    35821 gtcggtctgc aggcgcatac caatgcgtaa acggttgata aataccgagg caacctgatc
    35881 gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca ccagctgatt
    35941 tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac
    36001 cattttcttg tgaactcgct tgagtaacgc gacatcggtg gtattggcgg tatacatcaa
    36061 ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc
    36121 tacggtggcg tacttatcgt cactcagcgt gtgcttgata tacggagcct cacgcaattg
    36181 cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact gtgcttcttt
    36241 accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt
    36301 cccggcttta aagtgagaaa gatccggttc gatacgcagc agccattgaa aaacccgtgg
    36361 gcgattgatg atcttatcag cataaagctg ttcaccgagc gccagacgtc cggtccctgg
    36421 cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac
    36481 cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca agataatcaa
    36541 taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac
    36601 gttgctgacg aaaaggtgac atcgccacag gcacgtacgg gcatcactgg cattaacgca
    36661 ttacaaataa ccatctcatc tgcctgcaac gcctcctcca gcgaggcttg cacttcgaca
    36721 agctgataag aggattgtgc cagtaaacgg atacagaatt gtcgcataat gccgttaaca
    36781 cctgcctgat ccagtcgcgg cgtataaact acattgccct tccgccagaa caaattagcc
    36841 gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc
    36901 tgctcaagat gagagcgaat caatacttgc tcaagccgat taagatgttt aatacctgca
    36961 agatgaggat tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc
    37021 aaacggtcgt aatgtgcagg ataagccgta acggagagaa tccgcgttgc tggtccgctg
    37081 ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagcacacca
    37141 ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca gaagtcacag
    37201 gaaatcatca accgctgaca agcatcctgt agtcgcagga tatgcgccga taacaaactg
    37261 actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga
    37321 tcgcttactg ccagcgattc ctgcttataa ccgttaatta agaacatacc ggctccttat
    37381 cagagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata aaagctaaga
    37441 aaaaaggccc gcaagcggac cttttatatg ggtggaaaat gacaacttag atctttttaa
    37501 agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagcgtg tattccattc
    37561 cgctgacctg acgtgcttca tgcggtacga aatccagatc gcaaccttca tccgggttat
    37621 ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag tagatagatt
    37681 ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca
    37741 cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag
    37801 ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg actcgcttca atgcctgcat
    37861 cacgcagagc atttgccatt gccagagctg cgcctgcgcc attttctggc ggtgacgtca
    37921 tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc
    37981 gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga
    38041 agccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag
    38101 ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc
    38161 cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca tggccaatgt
    38221 tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca
    38281 tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga
    38341 tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc
    38401 caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc
    38461 cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag gcatccatct
    38521 tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa
    38581 atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg
    38641 caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc
    38701 cggtcacaac tacacgacgc ttagacacgt ttgtcctcca aggagggaaa aatgattcta
    38761 gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc
    38821 ctggtggccg ttgatgtaat caatggcagc ctgaacggtg gtgattttct cagcttcttc
    38881 gtccggaatc tcagtatcaa actcttcttc cagagccatt accagctcaa cggtgtcaag
    38941 agaatccgcg cccaggtctt caacgaaaga agcattgttg gtaacttctt cctgcttaac
    39001 gcccagctgt tcgccgataa ttttcttaac gcgttcttcg atagtgctca tactcttaaa
    39061 tttcctatca aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt
    39121 gcaactaaat cccggcaggt cttaccacga ttttacgtta ttttgaggcc ttacccccca
    39181 ataacgcaaa tcattttgca ctaattatta ctcataacca cgcagactgc gcgcaacatg
    39241 agcattttgt gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat gcaaagtttc
    39301 acccgtgatg taagctgctt cgtcggatgc caggaatgca accgcgttgg cgatttcctg
    39361 tgcgccgccg aggcgacccg caggaacctg cgccaggata cccgcacgct ggtcatcgct
    39421 cagcgcacgt gtcatgtccg tttcaataaa gcccggagca acaacgttta cagtaatacc
    39481 gcgtgacgca acttcgcgcg ccagtgattt actgaagccg atcaagcccg ctttcgccgc
    39541 agcgtagttg gcctgaccgc catttcccat ggtaccaacc acagaaccga tagtgataat
    39601 acgaccatga cgctttttca tcatagcgcg cattaccgct tttgacagac ggaaaacaga
    39661 tgaaaggtta gtttcgataa tatcgttcca ctcttcatct ttcattcgca ttaacaggtt
    39721 atcacgagtg ataccggcat tattgaccag gatatccact tcaccaaatt ctgcgcgaat
    39781 tttttccaga acagattcga tagatgccgg gtcggtcaca ttcaacatca gacctttgcc
    39841 gttggcacct aaataatcac tgatcgcctg agcgccattt tcactggtcg cagtgccaat
    39901 aactttcgcg ccacgggctg cgagcgtttc agcaattgcg cggccaattc cgcggcttgc
    39961 accggttacc agtgcgattt ttccttcaaa attcatgatt ttcctctttt aaagctcgag
    40021 cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg
    40081 tttcgtcagg ccagtaagca ccttgcccgg gccgacttca tagagatgtt ctacgccttg
    40141 cgctgccatg tactcaacag acttcgtcca ctgaaccggg ttatacaact gacgtaccag
    40201 tgcgtcacgg atggcatcac cattggtttc gcatttcaca tcaacgttat tcacaacaga
    40261 aactgttggt gcgttaaagg tgattttcgc taattctact gccagtttgt cggctgctgg
    40321 tttcatcagc gcacagtgag acggtacgct cactggtaac ggcagcgcgc gttttgcgcc
    40381 agcagcttta caggcagcgc cagcacgctc aaccgcttct ttatgaccgg caataaccac
    40441 ctgtcccgga gagttaaagt ttaccggaga aacgacctga ccttctgcag cttcttcaca
    40501 cgctttcgca ataggcgcat catccagacc gatgattgcc gccatagcgc ccgtgccttc
    40561 cggtacggct tcttgcatga acttaccgcg catctcaacc agacgcaccg catcagcgaa
    40621 atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac cggccatcat
    40681 tgccggtgct ttaccgccct gctgctgcca tacgcgatac agcgcaacag atgcagtcaa
    40741 cagcgctggc tgggtttgcc aggttttatt cagttcttca gctggcccct gctgggtcag
    40801 cgcccacagg tcgtagccca gcgccgcaga agcttcagca aacgtttctt cgacaattgg
    40861 atagctcgcc gccatatcag ccagcattcc aacggtttga gaaccctgtc cagggaacac
    40921 aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg cagagcccca
    40981 agtgaatcca ccgccaaagg cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc
    41041 gcgtacagct tcatccagcg cgcacgggac agaggccgca gaggtattac cgtggcgatc
    41101 cagcgtcacc acgacattgt ccatcgacat accgagtttt ttcgccgttg cactgataat
    41161 acgcaggtta gcctgatgcg gaaccagcca gtccagttga gaacggtcaa gattattcgc
    41221 cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc gcaaccttga agacttcgtt
    41281 gcccgccatc gtcagatgaa ttgaattctc tggattcacg cggtcggcgt ttggcagcgt
    41341 cagcaattca ccataactac cgtcggcatg cagatgggtg gaaatgattc ccggctcttc
    41401 agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa ataataatag tcccacgatc
    41461 tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg accagagcat acttcaccgc
    41521 cccagatttc acgtattgat cggctacgct taatgcatag gtgaaacctg cacaggctgc
    41581 tgcaacgtca aatgccgggc aacctttaat gtccaacatg ctttgaatct gacaagctgc
    41641 gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa tctggtcttt
    41701 ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt
    41761 ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa tccactcgtc
    41821 agaggtgtcc accatttttt ccaaatcggc gtttgtccgc acttgttcgg gcagatagct
    41881 gccagtacca ataatcttcg tatacatgta cgctcagtca cttttcggtt atataccgtc
    41941 acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg cagagttccg cttttgccac
    42001 cgtccagcag ctcaaaacca gctgggtata cagattccag gcgagcggca attcgctgag
    42061 gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat
    42121 tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc taacagacag gcgccgttat
    42181 actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta
    42241 ataacagtag ccaccacgac cgttttttcc cttcaccctg agatttcagc agagaaagga
    42301 acatcctgac aacaccttcc atcgtcttta acgtgacatt tcctgtaaag ccgtcacaaa
    42361 ccagcacatc tgtcttgcca gttaacaact cattggcttc aagatagccg atataattga
    42421 tagaagggat tgttttaagc accgctgagg catcccgaat actgtcaaga ccctttactt
    42481 cttcttcacc aatattgagc aacgccacgc gaggattggg aatttccacc acctcttcag
    42541 ccagaactga gcccataatg gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg
    42601 cccctaaatc gaggaccacc gttttgccct tttgctgatg tggtaatacc gtcaccagcg
    42661 ccggacgctc aatcccctcc aggggcttga gtaataattt tgccagcccc atcagtgccc
    42721 cggtattacc ggcactgaca caggcttgcg ctcgaccttc tttcaccagc tccagggcca
    42781 tacgcattga actcccacga ctggcgcgga tagcttgcga aggccgggca tcactggcga
    42841 taactgactg cgcaggaata atctgcagac gcgaacgttg ttcaaagtca gctttagcaa
    42901 gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag agtgagttgc gaattagaat
    42961 tcagtgcctg caatgctgca ggcactgtca cggaagggcc aaaatcccct cccatgacat
    43021 ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt
    43081 tgcctgggga aaatcctcac taagcttcat cacgcagacg cgtgattact tagcgatgac
    43141 cttgcggccg cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga
    43201 agttttgtct acagacaggc tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc
    43261 acgtttggaa cgggttggtt tattctgttg tacggccatg gaccttactc ctcaattacg
    43321 gtttatcccc gatccaacgg ggagcaccaa ttacttacgc tttaagctgg ctaatacggc
    43381 aaatgggttt ggcttttgca cttcttcagg cagttcacca aagaccatgt ccgcttcgga
    43441 cacttcacag tgttcagaat catgcaccgg aactaccggc aaggcgagga tgatttcatc
    43501 ttcaaccatt gcaagcagat cgatttcacc gaattcgtta acctcaatcg gttcatacgc
    43561 ttccggcagt gcttcagcct gttcgtctga acgcacagga ctaaaacaat acgttgtgta
    43621 gacctgatga gtaaacggct tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc
    43681 atcgccgttt aacactgcga gacgttggtt atcgatagcg aacgacatgg agcattccac
    43741 atcactgtcc acactgacta cggattcggc gacgcgctca acctgatcag gggtatagat
    43801 accctggtaa tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca ggggtaattt
    43861 taccttttgc atagggcgcg catattaact ttgtaacgtc atagagtcaa agaaaaaggc
    43921 agcctgtggt tgccttttgc caataattcg cacacattgc gggttactgc tttattttcg
    43981 ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg catagtttaa aatggctatc
    44041 atcaatacgc tatatctgat ggaaaaaaat atgcctaaac ttattttagc ctccacctcg
    44101 ccatggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc agcacctgag
    44161 gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg actggcacaa
    44221 gaaaaagcgc aatctctggc gtcacgttat ccggatcatt taattattgg ttcagaccag
    44281 gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg
    44341 caattacgca aagccagcgg caatatcgtc accttctata ctggactggc gttgtttaat
    44401 tcggcgaatg ggcatctaca aacagaagtg gagccttttg acgtccattt ccgtcaccta
    44461 agcgaggcgg agattgataa ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc
    44521 tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagagggacg tgaccctaac
    44581 acgctggttg gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac
    44641 ccgctgatgg gatagttgtt tgcgccggat gcggttacgt cgcatccggc aatcggtgca
    44701 gacggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca ggcttatatg
    44761 cttgtattag cgcgcgttac gcagcttttg caaacaacgc ttcaaacctt catccatcgg
    44821 cgcttcgata cgcatcacct caccggtccc cggatgggta aacttcaacg ctgcggcgtg
    44881 caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc
    44941 accgtagcga tcgtcaaagg caatcggatg acccgcatac tgtgtatgca cacggatctg
    45001 atgagtacgt ccggttaccg ggctacaacg caccagggtg gcaaatgcat agcgttcttc
    45061 cactttaaag cgtgtttccg acggtttgcc ttcctgactg acacgcacga tacgttcgcc
    45121 gctttgcaga atatttttca ataacggcgc ttgaacgctc ttcacatgcg actgccactg
    45181 accgcgcacc agcgccaggt aatctttttg cattcctttt tcacgtaatt gctcatgcag
    45241 agaacgcaac gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt cccggtcgag
    45301 acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac cttcgataac
    45361 gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat tcagcaccag
    45421 gatatgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt gcagatgtgg
    45481 cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt gggatacgca cctcatcacc
    45541 cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt cacctttacg
    45601 caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca aaaagttatc
    45661 gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg gagtctctgt
    45721 tttcatggtg ggcgattcta attagccaac aggattcgcg ccactcattt ttctatgctt
    45781 atatttagtt ttgcacctta ttacttcact acgtgatcac tttattgatg gttattaaac
    45841 caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg taaaagtcat
    45901 cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca tccttgttaa
    45961 aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc agcaatggcg
    46021 taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct taccggtaga
    46081 tggaaatatt tctggagagt aatacccagt ctgtttcttt gataattgcg ctgtttttcc
    46141 gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa ccgtgaggtt
    46201 ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc
    46261 agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg ttaatcaacg
    46321 caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg tatgacctgg
    46381 atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc
    46441 gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt cacggtttcc
    46501 tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct catggtcgtc
    46561 ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc gataaagaag
    46621 agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt agctatctgg
    46681 ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc
    46741 gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg
    46801 tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca atgggattta agcttccgcc
    46861 tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccggctccg ttcctgattc
    46921 atcaggagag caacgtaatc gttcgcgcct tccgcgatta tttacgtcag gatatcggcg
    46981 aaatccttat cgataacccg aaagtcctcg aactggcacg tcagcatatc gctgcattag
    47041 gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc
    47101 actaccagat cgagtcgcag atcgagtccg ccttccagcg tgaagtccgt ctgccatccg
    47161 gtggttccat tgttatcgac agcaccgaag cgttaacagc catcgacatc aactccgcac
    47221 gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa caccaacctc gaagccgccg
    47281 atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt atcgacttca
    47341 tcgacatgac gccagtacgc caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc
    47401 gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg ctggaaatgt
    47461 cccgccagcg cctgagccca tcactgggtg aatccagcca tcacgtctgc ccgcgctgct
    47521 ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct ctctattctg cgtttgatcg
    47581 aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct gtgccaatcg
    47641 cttcttatct gctgaatgaa aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg
    47701 gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac cccgcactac cacgtgttgc
    47761 gcgtgcgcaa aggggaagaa acgccgacct taagctacat gctgccgaag ctgcatgaag
    47821 aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtcct gaacaacctg
    47881 cactggcaac ctttgccatg ccggatgtac cgccagcgcc aaccccagct gaacctgccg
    47941 cgactgtcgt agccccagca ccgaaagctg caacggcaac accagcagct cctgcacaac
    48001 ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag
    48061 ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa accggaacgt caacaggatc
    48121 gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga gcgccgcgac acccgtagtg
    48181 aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc
    48241 agcaaactgc cgagacgcgt gagagccgtc agcaggctga ggtaacggaa aaagcacgta
    48301 ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac
    48361 gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt caggaaaccg
    48421 aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga
    48481 aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa
    48541 ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc acgcacagaa ctggtgaaag
    48601 tcccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac aatgctgata
    48661 accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga
    48721 gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt
    48781 tgaccgtagc gtgcacgtct ccggaattgg cctctggcaa agtctggatc cgctatccaa
    48841 ttgtacgtcc gcaagatgta caagttgaag agcagcgcga acaggaagaa gtacaagtgc
    48901 agccgatggt gactgaggtc cctgtcgccg ccgctgtcga acctgttgtt agcgcgccag
    48961 ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc ggttgccgaa ccgcaaccgg
    49021 aagtggttga aacgacgcat cctgaagtga ttgccgccgc ggtaactgaa cagccgcagg
    49081 tgattaccga gtctgatgtt gccgtagccc aggaagtcgc agaacacgca gaaccggtgg
    49141 ttgaaccgca ggaagagacg gcagatattg aagaagttgc cgaaactgct gaggttgtgg
    49201 ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag
    49261 aagttgaaac ggtggccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg
    49321 cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact
    49381 ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc
    49441 atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta
    49501 atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac atattaaaat
    49561 tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaat gcccgccccg
    49621 gcgatcccag agcgggcaga caaaacatac ccagtttcat gatatgtttt aaagcgagct
    49681 tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt atacgatgcc
    49741 tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc cacatcaacc
    49801 agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc tacctaagga atccagcgac
    49861 tccagtttgt tcagctgcgt gcctaattcc gcgcgaacgg taaggacatt attcagcgag
    49921 tttttcagtc cacggttggt tttatccaat gccgcagcgg cgatttcttt atccgcttcg
    49981 ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc aaaaagattg
    50041 gtttcagaag cgctaccgtc tggttccgct accgcgttac tggtaatact gtcgaaaatt
    50101 ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg cttaatactt
    50161 tctgttccgc caacataatc accgttagcc tcactaaacg gcgcagtctc tgttttataa
    50221 ccggcaaaaa tgtagcgccc attaccgtca gtggtgttcg ccagattcag caactggtca
    50281 cgaagcccct gaatatccgt agccagcgag gcccggtcat cgtcactcaa ggtgccattg
    50341 ctggcgtaga caattttttc ctgagcattc tggatagcag tggtgacctg gctaagtaca
    50401 ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt
    50461 tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc gtctgaaggg
    50521 ttaacgactc gcttacccgt cgacatctgc tcgccgtact tcatccattc tgcctgagaa
    50581 ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct
    50641 ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg
    50701 cattcgccag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga
    50761 caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc
    50821 tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt
    50881 cgttaaagga tttcgcaccg cccaccgttt tactgttgct ttgcagatcc agcagggcct
    50941 gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct cgccatcgcg attttcgctt
    51001 cgtcggtgat taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc
    51061 tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac
    51121 cgttggcatc tggtgtcgcc gtaaaagtgg tattgctggc aaggcgggtg acctgccact
    51181 gattattatc gaacgagatt ttgtaatctg tcgccagtac cacggaggca tcagttaccg
    51241 tggcaccgat cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga
    51301 tagtaaagaa atcttcacca gcatcgccgt tagcatcaaa tccggctttg tgttggctgt
    51361 tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat
    51421 cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga
    51481 tctcaatatt gcctgccgtc ccatcaacgt aagcgacagt cgtacgagaa gggtcggcgc
    51541 tggaaggaac tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg
    51601 catcgtgatg ttataagtac cgccatcctg aacgctgact tctacgccaa caatctggtt
    51661 taattcgctc accagttgat cgcgttgatc cagcagattg ttaggtgacg cccctgcccc
    51721 cacgcctgtc aggcgcgaga tttgatcgtt caggctggca atttgtttag cgtaattgtt
    51781 gatctgatca acgctggcac ctatcgcgat attgacctgt ttgtcctggt cgcgcagata
    51841 ttgatcggtg gttttaaact gattcaccaa cccttctgat ttcccaatca gcgcctggcg
    51901 cgccgccggg tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga aatcctgcat
    51961 ctgtgttgcc agcgaagagg tactggtgga gagcatattg tcgattttcg acatctgctc
    52021 atagcgggcg gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt tggtaataaa
    52081 cgtatcatac tcacgctgca caccagaaac gtagacgcca ttgccaaccc agccgccagc
    52141 gcccaacgtg ctattggcct gcgccataat agtggtttgg cgggtatatc cggcaacgtt
    52201 atagctggag atattattac ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct
    52261 catggcgtta ttgatcaagc tggacatgga ggttccttat aagccttcaa ttacagagta
    52321 ttatcggcag cgacccgccg gacttgagtt attcagaaca gattatcaat gttcatgctg
    52381 taggttttgc tcaccttgtc gcttatcgat ttcatctgct ggatcatgtt ggtgagttta
    52441 cgggcatagt gaggatcggt agcatagccc gcgtcctgta ggacctgcgc cccctgttcc
    52501 gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa
    52561 tccgacaatg cttccagata cgagctgtag acccgaaact tcgcttttac tttcttcgct
    52621 tcgccgtttt catattcagt cgtggtgatt tcagtgactt gccctttcca gttgccagag
    52681 gctttgacac caaacaggtt atagcttggc tcgccgtttt cgcggcggat ttgccgttgc
    52741 ccccaaccag attcaagcgc agcctgagcg aggatcaaat gatgtggcac accgctttgc
    52801 tggcttgcca gttgggcggg cagcgagagt tgcgcgagga atgctctact gtcacccggc
    52861 agcgaatcat cgtagttacg tggtacggcc ttttgcacca gctgcgaaag cgtctggttt
    52921 tgataacgca ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt ggactcctct
    52981 ggcaatggtt gttctggcgt catctgttta accatcatct ctgcaagccc cagacctttg
    53041 cccgccgtca tctgttgggc aatttgctgg tcatacatac tggtatacag acgagtgtgc
    53101 tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc
    53161 acgaacatcc cttccacctg acgggccacc ggacggatat ttgccgccgg atcttcgctc
    53221 gctttcgcct ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc cagtagtttg
    53281 ctgtcgctga tcatcagatg atttccagtt ttgcccgcag acatcccgca ctttgcattg
    53341 attgcaaaat agacatcaga tccatcggcg tagcgcccag cgcattgagc gcacgcacca
    53401 cgttattgag gctggcgctg gaacgtacgc tttgcagcga accgccgctc tggcgtaaat
    53461 cgatctgcgt ttgtggagta acaacagtct gtccaccacc aaacggtgta tctggctggc
    53521 tgacattggc ctgacgatta actgttactg agagatttcc ctgcgctacc gcgcagctgt
    53581 cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc actactttag
    53641 cgtcctgcgg ggtgacatta acctgcatat tctggatatc ggcaaggaaa cggacctggg
    53701 aactgttgcc acttggtacg cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc
    53761 catatccacg cacgcggttg atggtgtcag cgatttgctg cgccatgctg aaatcttcgt
    53821 cgttaagttg caaattaagg gtattcccga cgccaaactg gctgggcaat tcacgttcaa
    53881 taaccgcacc attggtgatc cgtccaccgt tcagttggtt aacctgaaca ctgctaccgc
    53941 ctgcggaggc tcctgcgccg ccaaccagaa tattgccctg cgccagcgca tacacctgac
    54001 tgtcaacgcc cttaagcggt gtcatcaaca acgtacctcc acgcaagctt ttggcattac
    54061 ccatggaaga aaccaccaca tcgatggttt gcccctgacg tccaaacgga ggaagtgacg
    54121 ctgtcaccat taccgcagcg acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa
    54181 ttcccagctg tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct
    54241 ggtcaccagt gccatccagc cccactacca agccatagcc aatcagtgag ttttgcctta
    54301 ccccctgaac actggtgaga tcgcgaatac gctcagcctg agccgccgtc gtaaccagta
    54361 gaagaattaa tgcagagaga aatttaatca ccacagcctc acttacattg gcgacaggtt
    54421 aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc cattgcctac
    54481 gtattcaata cgcgcatccg ccacctgagt agacggtacg gtattgctgc cgctgatagt
    54541 gcgtggatta accacgcccg agaagcgaat aaattcggta ccctgattaa tggcaatctg
    54601 tttttcaccc accacatgca ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa
    54661 cgtgccgcta aaggtattgc tggcattggc cccgcccttt ccgttgaacg tgttaccacc
    54721 ggaggcttcg acatcggcac gagcgttacc aaacagcccc tgcaaatagc gcggcacagt
    54781 atcaaagcca aaattagttt taccgtcacg gctggcattc gcagaggagc ttttgctggc
    54841 gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc
    54901 ttcaaacagc ggttgatagc catagttaat cggctgagca gactggaaaa tagaaccgtt
    54961 ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc gccccctgca ccagcggcgt
    55021 ggagggtatc caggcgcagc cggttagtga aagcaccaac aagctggaaa tggcataagt
    55081 atgcgcagcg tttttttgca tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc
    55141 caccggttat gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg
    55201 ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat attgaccagt tcttccgcca
    55261 cgttgacgtt agacgtttca acataccctt gatacagcag tcccgcgccg ttcaggcccg
    55321 gcgtgctttc gttcggtgca ccggaggatt gcgtttcggt gtagaggttt tcgccaatgc
    55381 tctccagtcc ggtatcattc ataaaggtgg tgagattgag ctgcccaacc tgaaccggag
    55441 ctgcctggcc ttgttgggtt acgctgacca cgccatcacg accgatggtg atacttaacg
    55501 cattcgccgg aatggtgatc gctggctgca cctgaaaacc accagccgtc accagctgcc
    55561 cgttctgatc cacctggaaa gagccgtcac ggatataggc tgatgagcca tctggcaaca
    55621 ttacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttattg gtctgcgaca
    55681 gattgccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg ccgatttgta
    55741 atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga atggtttgat
    55801 aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg acgtttgcca
    55861 ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt ttggcgatcc
    55921 ataatgaact gatcatataa tgtcctgtag tggattgctg acaagcagag cagaagcacg
    55981 cgacagtgtg aatttcccct caccctaacc ctctccccaa aggggcgagg gaatagtccg
    56041 cgcgctttta ttgcaatgtc atcaatcgtt caattaactc atcgacagca gttggttggc
    56101 acggcctgcg ttatcatcga cgctgctgat caccttcatc tgcatttcaa aacgccgcgc
    56161 gctggcaatc atgtcgctca ttgccgcaac ggcattgacg ttactgcctt ccagaacccc
    56221 cgacatcaca cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg cctgggtttc
    56281 tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac
    56341 cagtttcaga cgccctactg gcgcaactgt atttgccgga tcgcccggat tgagcgccga
    56401 gattgtgcca tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa ttggccccgc
    56461 ctcgcctatc accggatgcc cctgaattgt cagttgcccg gtgggatcaa cctgaatgct
    56521 gccattacgc gtataccctt cgctgccgtc agcggtctgc acggccagcc atcctgctgc
    56581 aacgcaacgt ccagcgggcg cgaggtgtaa tccatcttgc cgggcgtcat gtctgcaccc
    56641 ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac cggcactgcg
    56701 cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag attactggcg
    56761 gttaccgcct gttgattcag cgtctggctg gctgctccca tcgcagtata aattgcgtga
    56821 tccattgagc tatcccgtca gcgattagcg taagttaacc cgcgtgttga ggatctggtc
    56881 ctgggttttg atggtctggg cgttagactt atagttacgc tgggcaacga tcatattgac
    56941 cagttcttta ctgagatcga cgttggacgc ttccagcgca ccgttggtca gggtgccaaa
    57001 gtttcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac
    57061 gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc
    57121 cagcagttgg gtttgttcgt tggaattgtt gccgacaacc gtaccgtcat cattgatttg
    57181 ataactcacc agatcgcccg gtttgtagcc attctgggtg gttgccacaa tgttgttagc
    57241 gccggtattt tgctgcatgg agttgaggaa gctcagacta aacgtggcgg gttctgcacc
    57301 gttaattgcg ccggttgcga tattattcgc catcgcacca tccactaatg tgccattagc
    57361 attaaattcc agtgttgtcg ctgtcttcgc aatgctgttt ggatcactgc tatcctgggt
    57421 gtagacctgc cagttattat ccccggtctt cacaaaatag acgctcatgt catgagcatt
    57481 accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctggc
    57541 gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct gcatcgacgc
    57601 cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg tcggattcgc
    57661 cccttgctga atagtcggcg gcgtaccggt taccgggtaa cccgtcagct gtaaaccttg
    57721 cgtattcaac aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac
    57781 cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc
    57841 tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg caacttttac
    57901 ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa
    57961 gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc
    58021 gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta
    58081 aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt
    58141 accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag
    58201 ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg gcgcagtgct
    58261 gccatcagtc aacgaaccgt cccaggtgaa actgtgaact ccggcggtca gttcaccaat
    58321 atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa ctttgtctgc
    58381 cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc
    58441 ggttcccgca agaacagttg tgccggggat catcacgccg tgaccgatca ggttactggc
    58501 ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt
    58561 ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt tgttttccat
    58621 tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa aactgctttg
    58681 taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga cgccggtatt
    58741 tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg accgagcgta
    58801 agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg
    58861 cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg catttttacg
    58921 tagccctttg catctgccag cggattacct ggttcataaa ccagtttgtc cggggcctga
    58981 ctttcttaac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct
    59041 ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg ctatcagcat
    59101 ttgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac gccgacccgg
    59161 cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca
    59221 tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg ctgttatcgg
    59281 caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg
    59341 gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccggg atgtgttgcg
    59401 ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt
    59461 taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga
    59521 tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac
    59581 gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc
    59641 agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt
    59701 atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga
    59761 aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt ccgttaagta
    59821 cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc gcgggggtaa
    59881 gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca tgcgagcagc
    59941 cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct
    60001 gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat tatgtggttg
    60061 ccgcgatgcc tattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctgaaacgtg
    60121 gacgcctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg
    60181 ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat
    60241 ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg tttagcgcca
    60301 acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg gtgcgcatgg
    60361 tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt ataaacctgt
    60421 aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc
    60481 tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct gtaagcaccg
    60541 ttcaaccgcg cgaaaccacc gacgcgccgg taacgaacag ccgggcggca aaaacaaccg
    60601 cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca
    60661 gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac
    60721 taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag caagacttgc
    60781 agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc cgctgtgctt
    60841 aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg gcagatcaac
    60901 ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac gctggattac
    60961 ctcgaacagt tacgcaagaa agaacccaat acagcaaata gcgttgatat tagtcaacgc
    61021 tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg
    61081 ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag
    61141 gaaccgacgc tatatggggc gaacggtcag acctcaacac cccatcgcgg cagtaaaaag
    61201 atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt
    61261 ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc
    61321 aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga
    61381 ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac
    61441 caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt tctgcttacg
    61501 caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag
    61561 cccggcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta aaatcagcgt
    61621 aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa
    61681 cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt
    61741 catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc
    61801 tgaaagaatg cccaacgcaa ccgcactcgg cagtgccaac aggaaacaaa gacgcaaccc
    61861 ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga
    61921 cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gagggaaact ccattaagcg
    61981 gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt
    62041 gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca tctgtttcac
    62101 cacgcgcatt gctccagcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt
    62161 cttcaggtgc ggtagctgat acaccagctg caggatgccg ccgaccgtaa cagcccacgc
    62221 cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct
    62281 gatgttaagc agtgttggcg caaacgccgg aatcgagaag cggttccacg tattcagaat
    62341 cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag
    62401 ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat
    62461 cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa gtgtcagcag
    62521 gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc
    62581 cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa
    62641 gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct
    62701 ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac
    62761 ggcggccagc gattttaata aattcatcgg tgttctaatc cggacccacg ggcaaaacgc
    62821 ctgcaaggca ggcgctgaaa gaggcgaata gtctacctag attatggtga attgctaccg
    62881 ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt
    62941 gcgccagcac tgcctattcg ccggcggttt gcggaactgt ctgattttgc acgcattcaa
    63001 tgaagtgccg cgcacagccg acaaatccac gttgctcaag agtgctctgc caaccaggaa
    63061 tcggtttatt cactacaccc tgcccgcgct cctcacgcca ttcgcgcata tccgtaatgt
    63121 cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat
    63181 gcatacaagt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca aacagcattt
    63241 cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc
    63301 acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg
    63361 gcccgacgct attgctacga tgtttatcca ttcttagcga ggctgcggtg gcgagttgcg
    63421 tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt
    63481 ttttccgcgc cgccagttcc accagccttt cagcatcgcg cagattttct gccagcggtt
    63541 tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca aagtggctgg
    63601 cggtgctgga gtgcacaaaa accgcatcac aactggcggc aaggctggat aacgaatcgg
    63661 cataaggaat gcgccagctg tcacaaattg gcagggcttt ggcgcgcgta ggcgaccagg
    63721 ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa
    63781 tgccgcctaa tcccactacg ccgatacgta attttttcac tttaatctcc cagatgggcc
    63841 agcaacgaat caagacgttg tttcagttct gccacttcga tttccagggc ttcgacgcgg
    63901 gtctataaat caccgtcaac catgttcgac atagccgtta ccgccggctg atcttcaacc
    63961 tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc
    64021 accacaaaag gaccatcttc gcgattcgcc agttgttcca gcgtcgactc cacttccgcc
    64081 atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca
    64141 ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagttt cagatcgcca
    64201 aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa accgctcact
    64261 gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc ggattcactc
    64321 agattcatga ccggtttacg gttcgttttc tgattacagg ccgtgactac accattgact
    64381 gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aattacgcga
    64441 gcttccaagg cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc
    64501 tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc
    64561 tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt
    64621 gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat
    64681 ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata
    64741 accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg caacgccaat
    64801 aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa
    64861 ttcgttaatc atccctagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg
    64921 cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg ccagacgcca
    64981 ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg
    65041 cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg
    65101 ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa tgccattttc
    65161 tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata
    65221 ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt
    65281 ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg gtaaatattt
    65341 cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc
    65401 tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct
    65461 acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg
    65521 tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg
    65581 tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac
    65641 gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacaacgtgg
    65701 tcgttttttc tcgctgttga tgatgcagga cagtgccagt gcggtcattg gcgcattgtt
    65761 ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg ccgttttgtt
    65821 tgtgctatgt gcggcgttca atgcgtggtt gttaccggca tggaaactct ccaccgtacg
    65881 cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt
    65941 cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat
    66001 ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg
    66061 tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga
    66121 acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt
    66181 cagcggccta caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc
    66241 cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc gcggcagcta
    66301 tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggtgg
    66361 ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga tgatgctggg
    66421 aattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgccgc
    66481 gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga
    66541 aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc
    66601 ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt
    66661 tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat
    66721 tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga
    66781 taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct
    66841 gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt
    66901 tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc
    66961 gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga
    67021 tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga
    67081 agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg
    67141 gccaagccgc gttgctaatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt
    67201 atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc
    67261 gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg
    67321 atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat
    67381 tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac
    67441 aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg
    67501 gcgtggctga cgcccatatt gttctgacaa acttgaccag ccaaattggt cgcgaagagc
    67561 cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc
    67621 agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg
    67681 cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa
    67741 cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc
    67801 aacctgctta cgtcctgcgc gaagatggca gtcagggcga agcaatggcg aaaaaactgg
    67861 cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat taatgacgag
    67921 ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt
    67981 ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca tcccaggtat
    68041 taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa
    68101 ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc
    68161 cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac
    68221 ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta
    68281 atgagctgga gcgcggattt aatgaagccg tgttcaccgc tgcaaaactt tacccggcaa
    68341 acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg ccggtacttg
    68401 agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata
    68461 tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc
    68521 gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg
    68581 tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc
    68641 gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc atcctcaaac
    68701 gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgagtattcc
    68761 tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg
    68821 gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg
    68881 ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat ggcttgccgg
    68941 tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa agcatcgcac
    69001 tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac
    69061 aataaaaatc gtagcttcct gttgtcatta ggttatttta cctgtataaa taaccagtat
    69121 gttcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca
    69181 gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta tgcgtttcct
    69241 gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc ggttattggc
    69301 gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc
    69361 gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg atcgccccgg
    69421 cttttttatt ttacttacga attttgagta caaaatttct acacttcctc aaaataattg
    69481 cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata aatcttaaaa
    69541 gcagaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata
    69601 ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa
    69661 ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg
    69721 ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc
    69781 aaataagtcc gagcaggaag ggactgaagt tggtcagacg ctctggttaa ccactgatgt
    69841 tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt
    69901 cccggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc
    69961 accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa
    70021 ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt
    70081 aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc
    70141 ccatatgcca ccgcatcaac acggcagcca ccacggcgat acgcgattaa ttcgccatgc
    70201 ttatggtgaa ggcgaaaagt atgtcccgtt ggtcctccgc gcgcaaacgc tgtgggatga
    70261 actttcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca ttaacctcgg
    70321 cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa
    70381 cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac gcgtcccgga
    70441 caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa
    70501 aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac
    70561 cgcaattcgt catgacgatg atggcgtaac tattgaaacg gttgacggtg agtatcaggc
    70621 gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt
    70681 ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata gcgtgaagaa
    70741 taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc
    70801 agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga
    70861 tgaacgtgtt ccgtttgcgg aagtggccag tgatgggtca gaagccttcc cgttcttgcg
    70921 caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc
    70981 gcctgacgaa gattttatta tcgataccct acccggccac gataatacac tgctcattac
    71041 cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgccgattt
    71101 tgcgcaagac caaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata
    71161 atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg
    71221 ttgcactatc tcatcaataa tattcgcgag catttgatgc tttatctttt tctctgggga
    71281 ttgttggcaa ttatggatct gatctatgtg ttttattttt agttttccgc actgacataa
    71341 actgacaaat atttaatctc cctttcattt agcgtgaaaa attgaatgat attattcctg
    71401 cttctacagc actcaccaga catctctgat gctgaaccca ctaaacttat gaagattctt
    71461 tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaatttg ttatttttgt
    71521 gttgcaattc attctccgac acgccatttt ctttctctcg caaattcgta ttggatgcac
    71581 ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc ttccattggc
    71641 taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggtc acgctcagtt
    71701 attacgatgg ctggtatcac aaagcacccg aactgcataa aagcatcggt attttgttaa
    71761 tgatggggct ggttattcgc gttctgtggc gtgtcatatc tccaccgccg ggtccgctgc
    71821 cgagttattc gcccatgact cgtcttgccg caaaagctgg acatctcgca ttgtacctat
    71881 tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt aaaccgatca
    71941 gcgtttttgg ctggtttgac gtccccgcga cccttgccga cgctggcgca caggcagact
    72001 ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtactgtct gttatgcacg
    72061 gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag cgcatgctgg
    72121 gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt ggtttaacct
    72181 tcgcgtccct gatgttctct gccggttcag cggttgccgc cgattacaaa attgacaaag
    72241 aaggtcagca cgcctttgtt aacttccgca tacagcatct tggctatagc tggttatatg
    72301 gcacctttaa agatttcgac ggtactttta cctttgatga aaaaaatccg gctgccgata
    72361 aagtgaatgt gacaattaac accaccagcg tcgatactaa ccacgccgaa cgcgataaac
    72421 atcttcgcag tgcggatttt ctcaatacca caaaatatcc acaggcaaca ttcacctcca
    72481 ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg ctgaatggcg
    72541 taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac ccatggggcg
    72601 gtaaacgtgc aggctttgag gccgaaggca aaattaagct caaagacttc aatatcaaga
    72661 cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa ggcgtacagc
    72721 agaagtaaga gtgaaatgtt gccggatggc acgcatccgg caatgatatt tattctgttg
    72781 gatcaggaat acccagtgtt gtattcagac gtccacgaga cttattaaag atcttattgc
    72841 cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg ctttcttcgc
    72901 agcaaatctc actacaacaa cctttgtatt tttccgcgca tactggacac tgaataaaca
    72961 gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgcg ccgcactggt
    73021 ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca aaaacaaaat
    73081 ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg tattcaataa
    73141 ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag gcactggcct
    73201 tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta tgtgcctgca
    73261 tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt tcgagcgcgt
    73321 tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat agtgcatcgg
    73381 gatcgtcaag catggcgttc acttccgccg cttgcagata ctcaccaaca ttgctggcat
    73441 caaagtgagg atcgtcaata ccgtcggcaa caatgcgatc gcgtaccttc atgcgcagta
    73501 cccagaagga tttcccgtca tcatccaacg cgatattcag gcgtaaaccc tctaaagccg
    73561 ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgcaggt acgctgattt
    73621 gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc gcggtaaaca
    73681 gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac ttgtaaaacg
    73741 aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttaacgcg tcgttggaaa
    73801 tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag taaaaatccg
    73861 cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta catttgcgca
    73921 ttaaaaatta tttgttattt acaagcgcgg caatttcgcc cagtcttcag ccacaaattt
    73981 tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgtataggtt gaaaaacagg
    74041 attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg ttattggtta
    74101 acctggttgg gtattggcgt actttggttg gtcgtgcaat tgccctaccc gattatctac
    74161 cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg cgcaaaaatt
    74221 gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg ccgtaaaatg
    74281 gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggtat ggcgtggttc
    74341 tggccggacc gccgaatcgc ccgctggacg gaagtgatcg gcatggaaca cattcgtgac
    74401 gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac actggagctg
    74461 ggcgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc gaacgataat
    74521 ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc gatgctcgac
    74581 cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt ctggtacgca
    74641 ccagatcatg attacggccc gcgctcaagc gttttcgtcc cgttatttgc cgttgagcag
    74701 gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg tctggtgccc
    74761 ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct gccaccagag
    74821 tgttctccgc cactggatga tgccgaaact accgccgcgt ggatgaacaa agtggtcgaa
    74881 aaatgcatca tgatggcacc agagcagtat atgtggttac accgtcgctt taaaacacgc
    74941 ccggaaggcg ttccttcacg ctattaaatc tcccatgccg gatgcttcag aatggcatcc
    75001 ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat tgcgccccct
    75061 ggaagtcggg cgcataatta gtgtgtttat cttttcttct tatgttcacc gcgcctggcg
    75121 caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa ctggaaacga
    75181 aacctgatcg tcgcctggct aggctgtttt cttaccgggg ccgccttcag tctggtaatg
    75241 cccttcttac ccctctacgt tgagcagctt ggtgttaccg gccactccgc cctgaatatg
    75301 tggtccggta ttgtcttcag tataatgggt tcctaaacac atcattttag gtattgaata
    75361 tgagacgggc aatcagctac atcagatttt caagtgaacg acaactgaaa ggggactctg
    75421 tacgccgaca atctaagctg gttactgact ggcttgataa gaaccctgag ttttacttgg
    75481 attcatcctt gagctttaaa gatttgggga aaagtgcttt tagtggtaag cacctgaaag
    75541 gtggtctagg tgacttttta accgctatcg agaaagggct tgttaaagct ggtgatacgc
    75601 tactaatcga gagtttagac cgtctgtcac gtcaggatat tgatattgct tctgagctat
    75661 tacgccgtat cttaagggct ggtgtagatg tagtcacact gagcgatgga gaacattaca
    75721 cacgagaaag tttaaaagac ccgttagccc tgattaaaag cattctgata atgcagcggg
    75781 cgcatgagga aagtttgaga aagtctgagc gtgtccaagc tgcatggaac cgtaaaaaag
    75841 aactcatctc cgaaggtatc aaggtatct
//
LOCUS       DAJVRL010000004        71504 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVRL010000004 DAJVRL010000000
VERSION     DAJVRL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.004"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(750..2345)
                     /locus_tag="OQN02_000246"
                     /pseudo
     CDS             complement(750..2345)
                     /locus_tag="OQN02_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
     gene            complement(2700..4871)
                     /gene="glcB"
                     /locus_tag="OQN02_000247"
     CDS             complement(2700..4871)
                     /gene="glcB"
                     /locus_tag="OQN02_000247"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCS1641182.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4893..5297)
                     /locus_tag="OQN02_000248"
     CDS             complement(4893..5297)
                     /locus_tag="OQN02_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCS1641183.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5302..6521)
                     /gene="glcF"
                     /locus_tag="OQN02_000249"
                     /pseudo
     CDS             complement(5302..6521)
                     /gene="glcF"
                     /locus_tag="OQN02_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6532..7584)
                     /gene="glcE"
                     /locus_tag="OQN02_000250"
     CDS             complement(6532..7584)
                     /gene="glcE"
                     /locus_tag="OQN02_000250"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCS1641184.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7584..9083)
                     /gene="glcD"
                     /locus_tag="OQN02_000251"
                     /pseudo
     CDS             complement(7584..9083)
                     /gene="glcD"
                     /locus_tag="OQN02_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9334..10111
                     /gene="glcC"
                     /locus_tag="OQN02_000252"
                     /pseudo
     CDS             9334..10111
                     /gene="glcC"
                     /locus_tag="OQN02_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            complement(10235..11376)
                     /locus_tag="OQN02_000253"
                     /pseudo
     CDS             complement(10235..11376)
                     /locus_tag="OQN02_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11738..13468
                     /locus_tag="OQN02_000254"
     CDS             11738..13468
                     /locus_tag="OQN02_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCS1641185.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13465..14379
                     /locus_tag="OQN02_000255"
     CDS             13465..14379
                     /locus_tag="OQN02_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCS1641186.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14412..14660
                     /locus_tag="OQN02_000256"
     CDS             14412..14660
                     /locus_tag="OQN02_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1641187.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14660..15831
                     /locus_tag="OQN02_000257"
                     /pseudo
     CDS             14660..15831
                     /locus_tag="OQN02_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15867..16946)
                     /gene="lptG"
                     /locus_tag="OQN02_000258"
     CDS             complement(15867..16946)
                     /gene="lptG"
                     /locus_tag="OQN02_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1641188.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16943..18013)
                     /gene="lptF"
                     /locus_tag="OQN02_000259"
     CDS             complement(16943..18013)
                     /gene="lptF"
                     /locus_tag="OQN02_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1641189.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18044..18604)
                     /locus_tag="OQN02_000260"
     CDS             complement(18044..18604)
                     /locus_tag="OQN02_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1641190.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18616..19440)
                     /locus_tag="OQN02_000261"
     CDS             complement(18616..19440)
                     /locus_tag="OQN02_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641191.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19440..20789)
                     /locus_tag="OQN02_000262"
     CDS             complement(19440..20789)
                     /locus_tag="OQN02_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS1641192.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20835..21593)
                     /locus_tag="OQN02_000263"
     CDS             complement(20835..21593)
                     /locus_tag="OQN02_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641193.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21625..22338)
                     /locus_tag="OQN02_000264"
     CDS             complement(21625..22338)
                     /locus_tag="OQN02_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1641194.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22512..23204
                     /locus_tag="OQN02_000265"
     CDS             22512..23204
                     /locus_tag="OQN02_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1641195.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23253..24752)
                     /gene="pitB"
                     /locus_tag="OQN02_000266"
     CDS             complement(23253..24752)
                     /gene="pitB"
                     /locus_tag="OQN02_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS1641196.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25044..26903)
                     /gene="gss"
                     /locus_tag="OQN02_000267"
     CDS             complement(25044..26903)
                     /gene="gss"
                     /locus_tag="OQN02_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1641197.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27108..27974
                     /gene="yghU"
                     /locus_tag="OQN02_000268"
     CDS             27108..27974
                     /gene="yghU"
                     /locus_tag="OQN02_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1641198.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28097..28345)
                     /gene="hybG"
                     /locus_tag="OQN02_000269"
     CDS             complement(28097..28345)
                     /gene="hybG"
                     /locus_tag="OQN02_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1641199.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28479..28820)
                     /gene="hypA"
                     /locus_tag="OQN02_000270"
     CDS             complement(28479..28820)
                     /gene="hypA"
                     /locus_tag="OQN02_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1641200.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28813..29301)
                     /gene="hybE"
                     /locus_tag="OQN02_000271"
     CDS             complement(28813..29301)
                     /gene="hybE"
                     /locus_tag="OQN02_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1641201.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29294..29788)
                     /locus_tag="OQN02_000272"
     CDS             complement(29294..29788)
                     /locus_tag="OQN02_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1641202.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29788..31491)
                     /gene="hybC"
                     /locus_tag="OQN02_000273"
     CDS             complement(29788..31491)
                     /gene="hybC"
                     /locus_tag="OQN02_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1641203.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31488..32666)
                     /gene="hybB"
                     /locus_tag="OQN02_000274"
     CDS             complement(31488..32666)
                     /gene="hybB"
                     /locus_tag="OQN02_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1641204.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32656..33642)
                     /gene="hybA"
                     /locus_tag="OQN02_000275"
     CDS             complement(32656..33642)
                     /gene="hybA"
                     /locus_tag="OQN02_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1641205.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33645..34763)
                     /gene="hybO"
                     /locus_tag="OQN02_000276"
     CDS             complement(33645..34763)
                     /gene="hybO"
                     /locus_tag="OQN02_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1641206.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34952..35239)
                     /locus_tag="OQN02_000277"
     CDS             complement(34952..35239)
                     /locus_tag="OQN02_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1641207.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35358..36245)
                     /locus_tag="OQN02_000278"
     CDS             complement(35358..36245)
                     /locus_tag="OQN02_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1641208.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36402..37442
                     /gene="gpr"
                     /locus_tag="OQN02_000279"
     CDS             36402..37442
                     /gene="gpr"
                     /locus_tag="OQN02_000279"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1641209.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37482..37976)
                     /locus_tag="OQN02_000280"
     CDS             complement(37482..37976)
                     /locus_tag="OQN02_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1641210.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38167..39051
                     /gene="yghA"
                     /locus_tag="OQN02_000281"
     CDS             38167..39051
                     /gene="yghA"
                     /locus_tag="OQN02_000281"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1641211.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39323..39748)
                     /gene="exbD"
                     /locus_tag="OQN02_000282"
     CDS             complement(39323..39748)
                     /gene="exbD"
                     /locus_tag="OQN02_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1641212.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39755..40489)
                     /gene="exbB"
                     /locus_tag="OQN02_000283"
     CDS             complement(39755..40489)
                     /gene="exbB"
                     /locus_tag="OQN02_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1641213.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40741..41928
                     /gene="metC"
                     /locus_tag="OQN02_000284"
     CDS             40741..41928
                     /gene="metC"
                     /locus_tag="OQN02_000284"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1641214.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42068..42727
                     /locus_tag="OQN02_000285"
     CDS             42068..42727
                     /locus_tag="OQN02_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1641215.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42767..43666)
                     /gene="yqhC"
                     /locus_tag="OQN02_000286"
     CDS             complement(42767..43666)
                     /gene="yqhC"
                     /locus_tag="OQN02_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1641216.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43860..45023
                     /gene="yqhD"
                     /locus_tag="OQN02_000287"
     CDS             43860..45023
                     /gene="yqhD"
                     /locus_tag="OQN02_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1641217.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45128..45955
                     /gene="dkgA"
                     /locus_tag="OQN02_000288"
     CDS             45128..45955
                     /gene="dkgA"
                     /locus_tag="OQN02_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1641218.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46155..47081
                     /locus_tag="OQN02_000289"
     CDS             46155..47081
                     /locus_tag="OQN02_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1641219.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47132..47389
                     /gene="yqhH"
                     /locus_tag="OQN02_000290"
     CDS             47132..47389
                     /gene="yqhH"
                     /locus_tag="OQN02_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1641220.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47432..49651)
                     /locus_tag="OQN02_000291"
     CDS             complement(47432..49651)
                     /locus_tag="OQN02_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1641221.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49762..51174)
                     /gene="ftsP"
                     /locus_tag="OQN02_000292"
     CDS             complement(49762..51174)
                     /gene="ftsP"
                     /locus_tag="OQN02_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1641222.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51249..51986)
                     /gene="plsC"
                     /locus_tag="OQN02_000293"
                     /gene_synonym="parF"
     CDS             complement(51249..51986)
                     /gene="plsC"
                     /locus_tag="OQN02_000293"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1641223.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52220..54478)
                     /gene="parC"
                     /locus_tag="OQN02_000294"
     CDS             complement(52220..54478)
                     /gene="parC"
                     /locus_tag="OQN02_000294"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1641224.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55024..55506)
                     /locus_tag="OQN02_000295"
     CDS             complement(55024..55506)
                     /locus_tag="OQN02_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCS1641225.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55559..55951)
                     /gene="ygiW"
                     /locus_tag="OQN02_000296"
     CDS             complement(55559..55951)
                     /gene="ygiW"
                     /locus_tag="OQN02_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1641226.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56103..56762
                     /gene="qseB"
                     /locus_tag="OQN02_000297"
     CDS             56103..56762
                     /gene="qseB"
                     /locus_tag="OQN02_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1641227.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56759..58033
                     /gene="qseC"
                     /locus_tag="OQN02_000298"
     CDS             56759..58033
                     /gene="qseC"
                     /locus_tag="OQN02_000298"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1641228.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58079..58411)
                     /locus_tag="OQN02_000299"
     CDS             complement(58079..58411)
                     /locus_tag="OQN02_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCS1641229.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58730..59311
                     /gene="mdaB"
                     /locus_tag="OQN02_000300"
     CDS             58730..59311
                     /gene="mdaB"
                     /locus_tag="OQN02_000300"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1641230.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59342..59656
                     /locus_tag="OQN02_000301"
     CDS             59342..59656
                     /locus_tag="OQN02_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1641231.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59704..61596)
                     /gene="parE"
                     /locus_tag="OQN02_000302"
     CDS             complement(59704..61596)
                     /gene="parE"
                     /locus_tag="OQN02_000302"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1641232.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61625..62206)
                     /gene="yqiA"
                     /locus_tag="OQN02_000303"
     CDS             complement(61625..62206)
                     /gene="yqiA"
                     /locus_tag="OQN02_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1641233.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62206..63033)
                     /gene="cpdA"
                     /locus_tag="OQN02_000304"
     CDS             complement(62206..63033)
                     /gene="cpdA"
                     /locus_tag="OQN02_000304"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1641234.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63058..63480)
                     /locus_tag="OQN02_000305"
     CDS             complement(63058..63480)
                     /locus_tag="OQN02_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1641235.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63481..64110)
                     /gene="nudF"
                     /locus_tag="OQN02_000306"
     CDS             complement(63481..64110)
                     /gene="nudF"
                     /locus_tag="OQN02_000306"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1641236.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64315..65796
                     /gene="tolC"
                     /locus_tag="OQN02_000307"
     CDS             64315..65796
                     /gene="tolC"
                     /locus_tag="OQN02_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1641237.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65944..66615
                     /locus_tag="OQN02_000308"
     CDS             65944..66615
                     /locus_tag="OQN02_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1641238.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66621..67781
                     /locus_tag="OQN02_000309"
     CDS             66621..67781
                     /locus_tag="OQN02_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1641239.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67819..68607)
                     /gene="ygiD"
                     /locus_tag="OQN02_000310"
     CDS             complement(67819..68607)
                     /gene="ygiD"
                     /locus_tag="OQN02_000310"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1641240.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68750..69523
                     /gene="zupT"
                     /locus_tag="OQN02_000311"
     CDS             68750..69523
                     /gene="zupT"
                     /locus_tag="OQN02_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1641241.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69581..69751)
                     /locus_tag="OQN02_000312"
     CDS             complement(69581..69751)
                     /locus_tag="OQN02_000312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641242.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70012..70665)
                     /gene="ribB"
                     /locus_tag="OQN02_000313"
     CDS             complement(70012..70665)
                     /gene="ribB"
                     /locus_tag="OQN02_000313"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1641243.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70769..70917)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71039..71329
                     /gene="ubiK"
                     /locus_tag="OQN02_000314"
     CDS             71039..71329
                     /gene="ubiK"
                     /locus_tag="OQN02_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS1641244.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17839 a        19060 c        17663 g        16942 t
ORIGIN      
        1 tcatttattc acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca
       61 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      121 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      181 ttggagttaa aatacataca taatattaac atctgccaat atttcacttt ccgcaagacg
      241 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      301 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      361 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      421 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      481 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      541 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      601 gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag
      661 aagccaatcg taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt
      721 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      781 gcctgcagca gggtgataac gccgataacg ctggcaaaaa tcagactgtg cttcacggtg
      841 tagcggaaca gttaagattc tcggcccacc atgcccgtcg cggcgcaggc cacggcgata
      901 gattgcgggg agatcatctt gccagttacg ccgccgctgg tatttgctgc caccagcaga
      961 gtgtcagaga cgttgatttg ctgcgccgtg gtcgattgca gtgaaccaaa cagggcgttt
     1021 gaagaagtgt ccgacccggt aaggaatacg cccagccagc cgagaaacgg tgagaaaaac
     1081 gggaacatca cgcctgtacc tgccagtacc agcgccagcg tggtggacat gccggaatag
     1141 ttggtgacga aggcgaatgc cagcaccatg ccaatcgaca atatcggcca cttcagacta
     1201 attagcattt cggcaaagac gccaatccct ttcttgatgc ccacgccgag gatgaagata
     1261 gagataatcg ccgcaataaa aatagcggtg ccgccagccg agagagggtc gaatttaaac
     1321 accgcatcca ttggcgttgg ttgggcgaca atgggtgccg ctttcaacac ttgttgatgc
     1381 aaatgaggga tctggaaatt aatcaccagt gaataaaacg cgccgcccgg agcaaataac
     1441 gctttaaacg gcttcatggt ccagatggtg accagcaccg ttaagattaa aaacggtgac
     1501 cacgcacgaa tgatttgccc cagactatat tctgaaggca cgggaccgcc agaagatggc
     1561 ttatttacca ccatcgcacc tgcggattgc cccatgctga ttgccgtttc ggtatttttc
     1621 ggccgccaga ctttaagaaa taaagcgagt gagacgatac tcaccagcgc cgaagtaata
     1681 tccggcagtt ccggaccaat atagttagag gtaaagaact gagtgacagc gaagcttccc
     1741 ccagcaacca gcgccgctgg ccacgtctct ttcacccctt tccagccgtc catcattgct
     1801 accagccaga acggcacaag aaccgacagg aacggtaact gacgtcccgc cattgcgcca
     1861 atgtggaacg gatcgattcc cgttacctga ccggcgacca gaatcggcac gcccaacgca
     1921 ccaaacgcca ccgtcgcacc aaagccagca gctccttcca gcaacgcacc aaaggagaaa
     1981 ccaatcagta acacctgcaa acgctgatcg tcggtgatgg agataaccaa gctgcggata
     2041 atatcgaact gcccgctggc aacggttaat ttatacagga acaccgccgc gacaataatc
     2101 cacgcaattg gccataatcc ataaataaag ccatagcccg cagcagcaaa tgccatatca
     2161 atcggcattt taaaggcgaa tatcgcaatc aggatagata atataagggt tattgctcca
     2221 gcgacatgtc ctttcagacg taataccgcg agtgcaacga agaagaatat tatcgggatc
     2281 agggcgacca gagcggatag ccccagtcct cccatcggca tatacatttg ggtccaggta
     2341 accatattgt tcagtctctt attatcttta tatgcttgat atacttaagg ttgtaataag
     2401 caaaagagga ctgaactgta aaatataggc gttatacttt acagcaacag tacgccgcta
     2461 acgcaattgc tacctctggc ataacaagta tatcgggtaa gggtttctgt tccgcacacg
     2521 caggcgcaga gtatcgttaa gatgtccata ttgttgtttt aggcccgcta gtaatgcgct
     2581 acgggtattt aatattgtta aaccctgata atcgctccgg ttatttccgg gataaatgta
     2641 ctaccgcagt tactatcata gccccgacaa taaaacttgc cggggctttt ttgacgctat
     2701 taatgacttt ctttttcgcg taaacgccag gcgtgtaata acggttcggt ataaccgttt
     2761 ggctgtttca cgccgaggaa gattaaatcg ctggcagctt taaaagcaca cgagttagcg
     2821 aaattccccg ccatcggacg ataagccgga tcgccagcat tttgctgatc aaccactttc
     2881 gccatattct ccagcgacgc ctgcacctgt tctttggtca gaataccgtg acgtaaccag
     2941 ttggcgatat gctggctgga gatacgcagc gttgcgcggt cttccatcag cgccacgttg
     3001 tgaatatccg gcacttttga acaaccaatc ccctgctcca cccaacgcac cacgtacccc
     3061 agaatcccct gcacgttgtt atccagctct tgttggatct cttgcgccga ccagttagcg
     3121 ttttcagcca ccggaatagt cagcagatcg tccagcagcg gttcaaattc agcactgaac
     3181 tcggtctggg caatgttggc ttgcacgctc tgtacgttgg tatggtggta gtgcagcgca
     3241 tggagcgtag cagcggttgg tgacggaacc caggctgtgt ttgccccggc acgcagttgg
     3301 tcgcccttct ggctgtacat gtctgccatc aggtccggca ttgcccacat gcctttaccg
     3361 atttgcgctt taccgcgcag cccacagaac agaccggaaa gcacgttatt acgttcgtag
     3421 gctttgatcc acggcgtcga tttcatctgg tttttacgca gcatcgggcc agcttccatc
     3481 accgaatgca tttcatcgcc ggtacggtcg aggaaaccgg tattgatgaa cgccacgcgg
     3541 ttgcgcgcct gagcgataca gctacgcaag ttcagcgagg tccggcgttc ttcatccata
     3601 atgcccattt tcagggtatt cggtgccata ccgagcattg tctcaatgcg ggtaaacagt
     3661 ttgttggcga acgccacttc ctgcggaccg tgcattttcg gtttcacaat atagacgctg
     3721 ccagtgcgcg agtttttctg cacttttaaa tcatagaggg caatcgcgcc agtcatgacg
     3781 ccatcaagaa taccttccgg gatttcattg ccttcgctgt cccaaatcac aggaatggtc
     3841 atcaaatgac ccacgttgcg gataaacagc aacgagcgtc cgtgcagaga aatttcagag
     3901 ccatcggcgg cggtgtactg acgatcgtca ttcagtttac gtacgatttg ccgaccgttt
     3961 ttctccattt tctcttgcag agtcccctgc atcaggccca gcagattgcg gtacagcagg
     4021 attttatctt ccgcatcaac cgccgcgacc gaatcttcgc agtcgagaat ggtactgata
     4081 gcagcttcga cgataacatc gttgatgtgc gccggatcgt ctttgccaat ccgcccgttg
     4141 gcatcaattt gcagttcaat atgcaggccg ttatttttca gcaaaatgca ggtcggcgca
     4201 gcggcatcgc cacggtaacc gacaaactgt gctggagtac gtaacgtggt ttctttacca
     4261 tttttcaact ggatgcgtaa ttgtttatcg accaccttaa acgccaccac atcctgatag
     4321 ctgccgtttt ccagcggtag tgattcatcg aggaaacgtc gaacccaggc gataacctgc
     4381 tcaccgcgct gcggatcgta gccactgacc atcgcccctt cctgcgggat gatgtcgctg
     4441 ccgtataacg catcgtacag tgagccccag cgagcgttcg ccgcgttcag cgcgtagcgg
     4501 gcgttcattg ccggaaccac cagctgtggc cccgcctgac tggtgatttc gctgtcaatg
     4561 cccgtggttt ccaccgtcac gcgctccggt tgcggcacca ggtagcccag ttcacgaagg
     4621 aaagatttat aggccgcttt atcttttacc ggccccggat tgctgcgatg ccactcatca
     4681 agcgccgcct gaatgcgatc gcgttctgcc agcaactgac gattttctgg tgccagatcg
     4741 tgaacgatct catcaacatt gtgccagaat gcagcagcgt ccagttccac gcccggcaaa
     4801 acttctccat ccacaaaacg tttaaaattg gcgtcaatgc gtaaacggcc ctgggttatg
     4861 gtttgactca ttgtttctct cctcgttttc gcttatttcg ccaacaccgc tgctgccact
     4921 ttcgcgacct gcgcatcctg tgctccggtt aaaccagaaa cgcccacggc accaataatt
     4981 tgcccatcaa caacaaccgg tacgccgcct tccagcgacg tcagtaacgg cgcagtcacg
     5041 aacgcggtac gtccgttgtt caccatctct tcatagccct tagtttcacg acgccccagc
     5101 gcggcggtac gcgctttctc ctgggagatg taagccgcaa tcggcgcgca atcgtccatg
     5161 cgacttaacg ccagcagatg accgccgtca tcggcaacag caatggaaac agaccagtta
     5221 tttttctgcg cctcttcctg acctgcggca ataattgcac tcgccatttg ctggctaaga
     5281 atgactttag ttttcatttt tttattcctt ttcaagggct tgttctacaa tttcaatcca
     5341 gtgacgcaca gaggtacgac cggcgctcgc cagatgcgtc tggcaaccaa tgttggcggt
     5401 gacgatcatt tccggtttgc cgctttccag cgcattcatt ttgttatccc gcagctggcg
     5461 tgccagatcg ggatgcgtta acgcatatgt tcccgctgaa ccgcagcaca gatggctgtc
     5521 gggaacgtcc gttaaggtaa atccaagacg aagtaacact ttttccactt cgccgttcag
     5581 cttttgcgca tgttgtaggg tacacgggca gtggaaggcc agttttttat cgccgcgaat
     5641 tgccagtttt tccagcggtt cctcgcgcag aagttcgact aaatcgaccg ccagttcact
     5701 gacctgacgc gctttatcgg catataacgc atcgtttttc agcatctgcc catactcttt
     5761 gacgaacgcg ccgcaaccgc tggcggtttg caaaattgcc tcggcacctg cttcaatcgc
     5821 gggccaccag gcatcaatat tattgcgcgc ccgtgccaac cctttctcct gcgcattaag
     5881 atgatagtcc accgcgccac aacagcctgc ttcgttagct gacatgacgc tgatccccag
     5941 acgatccagc actcgcgcag ttgccgcgtt ggtgttgggc gaaagcgtag gctgggcgca
     6001 gccttccagc attaaaaccc gacgcttatg gcgcagcggc ggacgcggtt tagctttcac
     6061 cgtttcagca ggcagttttg ctctgacctg ttccggtaaa aacggtcgca gcaccagccc
     6121 tacctgcgtc agcgcacgga agaccgccgg acgcggcact acctggcgca atccttcgcg
     6181 cagcattcgc tccggcagtg ggcgtttcac tttctgctcg acaatatcac gcccgatatc
     6241 cagcaaattg tgatagcgca caccagaagg acaggtggtt tcacaattgc ggcaagtgag
     6301 gcagcgatcg agatgctcct gtgttttaag cgtgacttcg ttgccttcca gcacctgttt
     6361 aatcagatag atgcgcccgc gcggcccgtc cagttcatcg cccagaagct gataggttgg
     6421 gcaggttgcg gtacaaaatc cgcagtgaac acaggcgcac aggatgctgt cggcttccag
     6481 cgcgcgttct gccgcatctc ttcggttaat cgggtttgca tagcctgctc ctcaaagttc
     6541 cgcgtacatg cgaccggggt taaacacgcc gcaagggtcg agctgctgtt taagctgctg
     6601 gtgatagcgg aataaaggag ccgatagcgg ggcaaagcca ccatctccgg cactaaagcg
     6661 ggtcgcatga ccgccagcgt tgcgggcgat gcgatggatt tgattgtcat cggctgtcga
     6721 tttcagccag cgtaacgccc cgccccagtc gatcagttgc tcgccgggta aatccatcat
     6781 cggcgcatcg ctgggtaatg aaatgcgcca taaggtacct ggtaacgaga agaacggcag
     6841 ttgttgttca cgcaattgct gccagaactg acccgcaacc tcttcgccac ccagcagttc
     6901 acgcgctgct tttaccgatc cttcgccgcc ctcaaggcgg atccacaacg cattgtcgaa
     6961 gtaacataag ccactaatgg gtaatggctg gagttgccac tcggcgattt cactcatggc
     7021 ttcttgcagg ctgatttccc gacgcaggct cagagaggca cgcggtcgcg gtaacacttt
     7081 cattgagatt tcagtgagca cgccaagaca accatagctt ccggccatta accgtgagag
     7141 atcgtatccg gcaacgtttt tcatcacttc gccaccaaaa cgcagatgtt ttccagtgcc
     7201 ggtaatgatg cgcgtgccga ggacaaaatc gcggaccgaa ccgctccacg ggcgacgcgg
     7261 ccccgccagc ccgcaggcga ccatcccgcc ccaggtggct tcttcaccat aatgcggcgg
     7321 ctcgcaggga agcatttgcc cggcgctctc cagcgccgct tcaatcgcca ccagcggcgt
     7381 tccggcacga gcggttatca ccagctcggt cgggtcgtaa ttaacaatgc cgcgatgaca
     7441 acgcacatcc agcgtttgcc cggtgactgg gcgacctaaa aaggctttgc tattgctgcc
     7501 ctgaatcacc agcggcgttt tatcgctaat tgcctgattc acctgctcca gcagcgcctg
     7561 actgtaatca cactcgcgta gcatcaaaag cgctccagtt caggaaaagg taaatgaccg
     7621 tgatgcacat gcatggcacc aaattcagca cagcggtgta gcgtgggaat gtttttccca
     7681 gggttcagca aaccatcggg gtcaaacgcc gccttgaccg catggaaggt cgtgatttca
     7741 tcgctgttga actgggcgca catttgattg attttttctc gcccgatgcc atgttcccca
     7801 ctgatgctgc cgccaacttc aacgcagagt tcgaggatct tcccgcccag ctcttccgcg
     7861 cgggcaaatt caccaggttc gttggcatcg aaaaggatta acgggtgcat gttgccatct
     7921 ccggcatgaa agacgttggc aacacgtaaa tcatattgct gcgataaacg ggcaatgcct
     7981 tccagtacgc caggcagggc gcgacgcggg atggtgccat ccatgcagta gtaatccggg
     8041 gagatacgtc ctaccgccgg gaacgcattt ttgcgaccgg cccagaaacg tacgcgctct
     8101 gcttcgtcct gtgccaaacg gacgtcagtc gcgcccgctt tcaacaagat gtagttaacc
     8161 cgctcgcagt cttcctgtac gtcagactcc acgccgtcca gttcgcataa caaaatcgct
     8221 tcggcgtcga cgggataacc ggcatgaata aaatcttccg ccgcgcggat cgacaggtta
     8281 tccatcatct ccagcccgcc ggggataatg ccattggcga tgatgtcacc aaccgcaagt
     8341 ccggcttttt ctaccgagtc aaagctggct aacagaaccc gcgccacggg cggcttcggc
     8401 agcagtttta ccgtcacttc ggtggtcacg ccgagcatac cttccgatcc ggtgaacagc
     8461 gccagcaggt caaaaccagg tgaatccagc gcgtccgatc ccagcgtcag cgcctcgccg
     8521 tccagcgttt gcacttcaat tttcagcagg ttatgtacgg tcagaccata tttcaggcag
     8581 tggacgccac cggcattttc cgccacattg ccgccaattg aacaggcgat ttgtgaggaa
     8641 ggatccggcg cgtaatagag attatgcggc gcaacggcct aggagatcgc caggttacat
     8701 acgcccggct gcacgcgcgc gcggcgacca acggggttaa tgtcgaggct ctctttaaag
     8761 cgcgccatca ccaacaacac acctttttcc agcggcagcg cgccaccaga aagcccggtg
     8821 cctgcaccac gggtcaccac cggtacacgc aggcgatggc agacagccag aatcgctgtc
     8881 acctgttcca tttgcttagg cagaacaacc agtaatggac gcgtgcgata cgcgctcaac
     8941 ccgtcacact cgtaaggaat gatctcctca tcggtatgca ggatctcaag tccagggaca
     9001 tgctcacgca gtgccatcag taccgatgtg cggtcgacat cgggtaaagc gccatcaaga
     9061 cgctcttcgt acaagatgct catgagtagg cttcgctttg ttgtgttgtg tggcagctga
     9121 tttttgcgcg ctgcttctgt gaacagttat taagcgggct tttcgttttc gtctatctct
     9181 ttagctaccg gtcagaccat tttttttcca gctctgtgac cttgtcttgg ttaacttaat
     9241 gttaaattga tgtaacataa tcacttgcgt gatgtgcgtg ttttgcgagt taagaacaga
     9301 aaaattggtc ctacctgtgc acgaggtccg ggaatgaaag atgaacgtcg ccctatttgc
     9361 gaagtggttg cagagagtat cgaacggtta attatcgacg gcgtactgaa ggtcggtcag
     9421 ccgcttccct cggaacgtcg actgtgtgaa aagctcggct tctcacgctc cgcactgcgt
     9481 gaagggctga ccgtgctgcg cgggcgcggg attattgaaa cggcgcaggg tcgcgattct
     9541 catgtcgcac ggcttaatcg ggagcaggac accagcccgc tgatccatct gttcagtacg
     9601 cagccgcgaa cgctgtacga tctgctcgac gttcgcgcat tactggaggg cgaatcggca
     9661 aggctggcgg caacgctggg aacgcaggct gattttgttg tgataacccg ctgttatgaa
     9721 aaaatgctcg ccgccagtga gaaccacaaa gagatttcgc tgatcgaaca tgcgcagttg
     9781 gatcacgctt tccatctcgc catttgtcag gcttctcaca atcaggtgct ggtgtttacg
     9841 ctgcaatcat tgaccgatct gatgtttaat tcagtgtttg ccagcgtaaa taatctctac
     9901 catcgaccac agcaaaaaaa gcagatcgat cgccagcatg cgcggatcta caacgcggtg
     9961 ttgcagcggc tgccgcacgt cgctcagcgc gcagcgcgca gcgcgcgatc atgtacggac
    10021 cgtgaaaaag aatctccacg atatcgagct ggaaggccac catttgattc gctcggcggt
    10081 gccgctggag atgaacaagg tgggaatgtg agcagagttt taccctatcc ccaaggcgag
    10141 gggaccgccc ctgcacttaa caggatctgg tgttaaatct gttcatctat accctctccc
    10201 gaatgaccgc ccctgcgcta aacaggatct ggtattaaat ttgcttttca tacaatcggt
    10261 aacgcttgta cggctccgcc ccaatgcgtt ccagcatgtt attcatgcct gtattggttt
    10321 cgaggatcca tgacatctcc agcgcatcga tcttccggcg ggcaaacgga tcgcgtaagg
    10381 cttcaatcaa taacagcgca atcaccgggc cgatgcggct gaactgatac tcgtcgcgca
    10441 cgcccatcag cggtactcgc gcagttcgca caccgctgac tttcaaacgc cacagcaatt
    10501 ttgcccagcc gaagggaaag agcgatccgt tcagatcggc aatcgcctcg ttgatgttag
    10561 gcaagccaca ataaacgcgc agggtgcaga atcaatctca gcgatgtaga tcatatcgtc
    10621 cggcaccaga tatttaagtt ggtcgcccat ggtcgcgaat tcatgttcgg taaacggcac
    10681 aaatccccag ttgtgctgcc agccagagtt gaaaatctca cgcaggatct gcatctcttc
    10741 ggcaaaccgc tgacgattga tgcagcgaat ggtcaccttt ttgcgcacct gatccatcag
    10801 tttttttagc gccggagaga aagtgagatc ggttcgctgc atccaccacg ccagtaaatc
    10861 aatgcctttg tgataaccca gttgttcaat atgcgcggca taccacggtt tgccgtgtgg
    10921 catcatcgca cagggtggtg tgtcaaaacc ttcaatcagt aatccgcttt cctgattgat
    10981 attcaggctg aaaggcccgc tgatcttact tgcaccttgt gacttcaacc acgcttccgc
    11041 tgcgccaaac aacgcggcaa aatcctgcgg atcatcaatg gcgtcaatca tgccgaaatg
    11101 accggtatct tcaccgtaac gctcacggtg caaggtatct atttgcgcgg taatacgccc
    11161 aactatctgc cccgcttttt ttgccaccca cgcctgccag atgatatggt ccgtccccgg
    11221 atttttcgca gacaaatgct cgttgcgttc aatgaataaa gggggtatcc agtttggatc
    11281 gtcgggataa agtgatgacg gaaaagcgat aaatgcctta aggtcatttt tattaattac
    11341 tttttcaatt ttaatcatca gcagatcgca ttccatatct ctgtggataa caaataatta
    11401 atggtgaaaa acaggttgca acagcaacga gcggcatctt aataatcaac tcaatgaata
    11461 caaacgacga ataaatagta ttaattaatg attatgataa ggtcagaggc tacacagaaa
    11521 cgcctcaatg ccttttgtca atttaaggcg accggacaag gcttcttctt aatatattca
    11581 taaattaaat atcattcata ataaaaacaa aaaacttaaa ctcattattt aaatcaatgt
    11641 tcattttcac accgatgaaa atactatctc atttatcttt tatgcctata taatccaccg
    11701 cattattgtc attttatttt tgatatccaa ttttttcgtg gtgtatatgt ctaataaaat
    11761 ctttacgcat tccctaccta tgcgctatgc cgattttcca acgctggttg atgctttgga
    11821 ctacgccgct ctgagtagag ccggaatgaa tttttatgac agacgttgcc aacttgaaga
    11881 tcaactggaa tatcagacat taaaaacgcg tgccgaagct ggtgcgaagc ggttgttatc
    11941 gctgaacctg aaaaaaggcg aacgcgtggc gctgattgcc gaaacgagta gcgggtttgt
    12001 agaggctttt tttgcctgtc agtatgctgg cttagtcgcc gtcccgttgg cgattccaat
    12061 gggcgtcggt cagcgggatt cctggagcgc caaactccag ggtttactgg caagttgcca
    12121 gcccgcagcc attatcactg gtgatgagtg gttgccactg gtcaatgccg cgacgcataa
    12181 caaccccgaa ttacatgttt taagccacgc ttggtttaag gcattaccgg aagccgatgt
    12241 tgcgctccag cgtccagttc cgaacgatat cgcctacctc cagtacacct ccggcagcac
    12301 ccgttttccc cgtggcgtca ttatcaccca tcgcgaagtg atggctaatc tacgtgctat
    12361 aagccacgat ggcattaaat tacgccctag cgaccgctgc gtctcctggc agcctttcta
    12421 ccatgatatg ggactggtcg gctttctcct gccccccgtc gccacgcaac tttcagtaga
    12481 ttatttgcgc actcaggatt ttgccatgcg tcctttgcaa tggcttaaat tgatcagtaa
    12541 aaatcgcggc accgtttccg ttgcgccgcc gtttggctat gaattgtgcc aacgccgcgt
    12601 gaatgaaaaa gatctcgctg aactggatct ttcctgctgg cgcgtcgctg gtattggtgc
    12661 tgagccgatc tccgcagaac aactccatca attcgctgaa tgtttccgtc aggttaactt
    12721 tgacaataaa actttcatgc cgtgctacgg actggcagaa aatgcgctgg ctgtcagctt
    12781 ctctgatgaa gcctccgggg ttgtggttaa cgaagtggat cgcgacatcc tcgaatatca
    12841 gggtaaagcc gtcgcgccgg gtgcagagac acgcgccgta tcgactttcg tcaactgcgg
    12901 caaagcgttg ccggaacatg gtattgaaat ccgcaatgaa gcaggtatgc cggtcgcgga
    12961 acgtgtggta ggccatattt gcatctccgg tcccagtctg atgagcggtt actttggcga
    13021 ccaggtttcg caagacgaga ttgccgcgac gggctggtta gacaccggcg acctcggtta
    13081 tctgctggac ggttatctgt atgtcaccgg acgcattaaa gatctgatta ttattcgtgg
    13141 ccgtaatatc tggccgcagg atattgaata tatagcggaa caggaaccgg aaattcattc
    13201 tggcgatgcg attgcttttg ttaccgccca ggaaaaaatc attttgcaga tccagtgtcg
    13261 gatcagcgac gaagaacgtc gcgggcagct tatccacgcg ctggcggcac ggatccaaag
    13321 cgaatttggc gtgaccgcgg ctatcgatct gttgccgccc cacagtattc cccgaacgtc
    13381 ctccggcaag cctgcccgtg cggaagcgaa aaaacgttat cagaaggctt atgctgccag
    13441 tcttaatgtg caggaatccc tggcataaat caaactgtcg cggtgacggg cgctaccggg
    13501 tttatcggta aatatattat cgataacctg ctcgcccgcg gctttcacgt tcgcgcattg
    13561 acgcgtactg cccgcgctca cgtcaatgat aatctcacct gggtgcgcgg ttcgctggaa
    13621 gatactcatt cacttagcga gctggttgcc ggagccagcg tagtggtcca ttgcgccggg
    13681 caagtgcgcg ggcacaaaga agagattttc acccgctgta acgttgacgg cagcctgcgc
    13741 ctgatgcaag cagcaaaaga gagcggcttt tgccaacgtt ttctgtttat ctcttcgctg
    13801 gcggcgcgcc atcccgagct ctcctggtat gcaaaatcca aatacgtcgc cgagcaacga
    13861 ctggcagcaa tggcggacga aattacgctg ggcgtttttc gcccgacagc cgtgtatggt
    13921 cccggcgata aagagttaaa accgctgttt gactggatgc tacgcggcct gctgccacga
    13981 cttggtgcgc cagatacaca actctctttt cttcacgtca ccgattttgc gcaagcagtg
    14041 ggtcagtggt taagcgccga aactgtacag acgcaaacct atgaattatg cgatggcgtc
    14101 gccggtggct atgactggca acgcgtacga cagcttgccg ccaaagcccg ttgtggttcc
    14161 gtgcgaatgg ttgacattcc tctgccggta ctcacaggcc ttgcggatat cagtaccgcg
    14221 ttgagtcgcc tggcaggtaa agaacctatg ctgacccgct cgaaaattcg tgaattaacc
    14281 cacgccgact ggtcggcaag taataaccgt atttctgaag atatcaattg gtttcccggg
    14341 attagcctgg aacacgcatt acgcaacggg ctattttgat tgtcttgttt taaaggtgtg
    14401 ttaacgtttc tatggtaaat cgtgaaatag taatggatta catcctttcc tgtttacagg
    14461 atttggtcga aaacggtgtg gaaattaagc ccgacagcga tctcgtcaac gatcttggcc
    14521 tggagtcgat caaagtgatg gatctcttaa tgatgctgga agacagattc gatatttcca
    14581 ttcctattaa tattctgctg gatgtcaaaa cccctgcgca attaatggaa actctacttc
    14641 catggctgga gaataaataa tggggctata cgataaatat gcgcgccttg ctggcgagcg
    14701 tctgcaattt agtgacaacg ggttaacacc gtttggcacc tgtattgatg aagtgtattc
    14761 cgccaccgaa ggccggatcg gcaataaaaa agtgattctg gccgggacca ataattatct
    14821 cggtttaacc tttaatcatg atgccatcag cgaaggtcag gccgcgttag cggcccaagg
    14881 caccggcacc accggttctc gtatggcaaa cggcagttat gcgccgcacc tggcgttaga
    14941 gaaagagatt gccgagtttt tcaatctccc taccgctatc gtttttcaac cggttatacc
    15001 gctaatttag gcgtcatcag cgcacttgcc gaccataacg ccgtggtgtt actggatgcc
    15061 gacagccacg ccagtattta tgacgcctgt tctctcggtg gcgcggaaat tattcgtttc
    15121 cgccataacg atgctaaaga tctggaacgg cgcatggttc gcctcggtga acgggcgaaa
    15181 gaggcgatta tcatcgttga gggcatctac agcatgctcg gtgacgttgc gccgctggcg
    15241 gaaattgttg atatcaaacg tcgtctcggt ggttatttga ttgtcgatga agcgcattcg
    15301 tttggcgtgt taggcgcaac ggggcgcgga ctggctgaag ctgtcggcgt tgaagatgat
    15361 gtggatatta tcgttggtac tttcagtaaa agtctggcct cgattggcgg ttttgctgtc
    15421 ggttcggaag cgatggaagt gctgcgctac ggcagccgcc cgtatatttt taccgcctct
    15481 ccttctccgt cctgcattgc gaccgtgcgc tcgtcgttaa gaactattgc cacacaacca
    15541 gagctgcggc aaaagctgat ggataacgcc aaccatctgt atgacggtct gcaaaaactg
    15601 ggttatgagt taagttcaca tattagcccg gtagtgccag taataatcgg ttcgaaagag
    15661 gaaggtttac gcatctggcg cgagcttatc tcgccgggcg tgtatgtgaa tcttatcctg
    15721 ccgcctgccg cgcctgcagg catcacgctg ctgcgctgta gcgtaaatgc tgcccacagt
    15781 cgtgagcaaa tcgatgccat cattcaggca tttgctactc tgaagcagta atgtgagccc
    15841 gccgtggtgt ttgcgccacg gcgggtttaa tgttgtctgt cataccaata caccaggctc
    15901 aacgccacca acacaaacgc taccggtaaa ccgagcgcgg taacaggtgc gcttaacgca
    15961 aatagcaatc ccagattaac catgatttga tagctgatcc aggtgagtaa gcccacgatg
    16021 acgcctacag cgagacggct acccatccca ggcgaacgcg gggcactaaa ggtaaacggc
    16081 acagccagca aaatcatcgc cagggtcagg atgggttgcc ccagtttttc ccacagtgca
    16141 aggcggtatt cgctgctggg ttgcccggtt tcctgcaaat aatggatgta atgattaagc
    16201 tgacggacag aaaaagtatt ccccggcatt gataactctt caagattcat actggtgaag
    16261 gccgattgcc aggcaagatt atctaatgtt tccagcgttt ctttgccatt cagccatttt
    16321 ttatgattca caccatgcag gacccacgtt ttgtcgtctt caatggtggc actgcgtgca
    16381 taaatgtagg attcaagcga gagatcgtcg cgataatgaa atatctccac gcccaccggc
    16441 tggccctgct cgttcagcga tttcaccgtc acaaattcat taccacgcct ggcccacagc
    16501 atattgccgg taatgtcatc gtcctccccc aacgcggtgg cggtcgattt gatttgcaac
    16561 gcctgttgct gcaatggcga cgccacccac tcatctatcg cgcctaacga aacagtccac
    16621 aatatcccgg caaccagcgc caccagtgca atacggaaga tagaaaaccc cgtgctgcga
    16681 atggcggtaa gctcactgtt tttcgataac tgccccagtc caacaatacc acccaataac
    16741 gcgataaacg gcgaaagttc gaccagtgtg cgcggtaagg tcattagcac caccagcacc
    16801 gcctgagtcc agcgataacc gcccgggctg acaccatcca gttcgttaat caggttgaag
    16861 gtggtaaaaa gcggcagcaa tagccctgca gcggcggcaa aaccgagaaa gagatgacgg
    16921 attaaatagc gactgaaaac attcatcggg aaaatttctg cagcaaagag aagtcacgtg
    16981 cgaccagtat cagcaggccc attagcatga gtccgggcac taaccacaaa ccaggaatga
    17041 ggggaatcgc gccgttagcc accagtgtac ggcagacgtc gccgccataa aaaatggcaa
    17101 taaacagcaa cgtcagtggc aataacgttg aaaatcgccc ttgccgcggt tttacccgac
    17161 ttaatgaaat ggccagcagc gccatcaaca atgcggtcag gccacggctt tgtcgccatt
    17221 gcagttcggc atgatcggca ggaaaggcgg agcgcgccag ctccgtgacc gatttcgctt
    17281 tgcgtttgac gttcgggctt tgatccagcg gattcagatg taattgcaga ttacggtaga
    17341 tctgttcgtt gtcgtcacgc ccctgatgat cgagaagata ggcagtgccg ttgtgcaaca
    17401 taacggtcgg tttctcgggt gatgggtcaa ccacatcaac cgaaccggcg cggaaaatac
    17461 gggttcgatt ggcagtagaa gtataaatca gcgcgtcagt caggcgatta ttatcctgat
    17521 caaccgtctg cgaaaggatc attcgtccgt tatcgttagt gttaaatttc tttgcccgca
    17581 actggcgaac atccagctcc gactgcgact gctgctccag ttgataaatc tgcgcataag
    17641 cccacggacg accatacatc gacagaaggg tgacaaaaat acttagcggt atcgccagat
    17701 aaagaacggc tttgtacaag cgtcccggac tgccccccgc cgcagagata gcggtaattt
    17761 ccgagtcggt atacatctgc cctagcgtca cgccaactga cacatacaga ccaacaggta
    17821 acaacatctc cagtgcaatc agtactttgt agaaaacgat atccagcaca acatcaagag
    17881 ctaatgtccc atttgccgct tcggtcagat aacgctgtgc ggagtagctg gcgaaaataa
    17941 agatcagaaa accgacgata atcagcacca gacggcgcgt accacgcatg atgtagtgtt
    18001 caaccagctt catctcacta acgccctcaa tcctttgagt tttttattca ttgatcagtt
    18061 tctgcgccaa cgcaatgaca tgatctacct cttccggcgt caggctgccg tcctttgccc
    18121 actgaatttg cccggcttta ttcagcacca gaatggttga actctgctcc ggcaagcgcc
    18181 aggcaacgcg tccctgtcca ttgccgtcaa taacgaattg cgcccacgga tagcggcgtt
    18241 tatttttttc gattttgcca acaacaaaat agccagtacc aaagatggca tcatcggtgt
    18301 tcacaatggt ggtgggctga aaacgatcct gcggaaagtt tgccgcttca accgctttaa
    18361 ttaacagtga gttctttttc ttcgccgact tacgcccggc gatatactga ataatgcgga
    18421 cttttcccgc taattgtgtg ctgttccact tttggtagtg aatttcgtcg tcgctattca
    18481 gaagcagctc gccgccttcc tggatataaa caggggtaac cgttttgcca gtgacgaaat
    18541 tgtgtgcctg cacacccggg gctaagagca tgataatcaa cgcaataatc aaacgagatg
    18601 acatcattgc tccggctata gcggaaaacg cgaaatcagc agctctaagc gctgacaaat
    18661 atcgctatcc agcgccaacg gtgcataata cgactggcgg ttttccatat catccgccac
    18721 catgtcgtcg tgtttatctc ccatcaggcc attaatggcg gcgaggaaac gggaaatctg
    18781 taacgcctct ttattttcag gatcaagcga tcttaaggtg tcgtaataag aaacctgctg
    18841 gctgatgatt tttcgcacat catgaaattc cctgccggtt agtcgcggtt tggcaataat
    18901 ctctttcagg gtatgcattt gcttttgccg ataggcaata aaactgctta cgctttctaa
    18961 ctggctatgg cgtaatgaat agttcaccaa cgaccagacc ggagagctta aataaatccg
    19021 taacaggttg ccgtaatacg acacaatatt tttcttaccg ttacgaaagc cgtcctgaaa
    19081 acgccccaga aaaacggtcg ttttgccgaa taaaaatccg gcctgatgct tctttaaata
    19141 cagccgttgc gcaaaacgca ggtgcttgta gcgcgctcgc atgtatttaa attgtttgcg
    19201 ctcatcagca gtcagctcat cgcctttggc ttgtttgcga actaaacgca gtaattcctg
    19261 acgatcaacg ccattgagcc agaattgcag gcataacgcg taattatcat gaatgatttc
    19321 ggtggtacaa cttagtgcaa tagtcggcgg caaactaacc tgtgcatcaa cgacatcatc
    19381 ttcttcaatc gcaacaaata gacgctcgct cgcctcaagc gggaaatatt ttctcatcat
    19441 caggatttct ctccggctgc gttataaagc aaggaatggc gctgaaaaaa tgcctttaag
    19501 gttggaatta acgagagcgc cacgtccaga cattgcccac cgaagtaagc aaacgctgct
    19561 cccagcacac caaacagatg acagaacatg aacagcaata cgatgtagcc gattgaggcg
    19621 atggtttgcg ccacgagaaa agcgcgctgt tttcccgcca tcaatagcaa tgactcctgc
    19681 gggaagccca gcatcgaaat cacaattgcg cccagcatca cctgaatcag gtcatacgct
    19741 tcgagatact taacgccaaa caccagcgaa atgagcggct tgccgacgat caacaccgcg
    19801 agagccacca gaataccgat tccgcccgcc agtaaaccgg acttcacacc taacaaccac
    19861 ggtctggtgg tgcgcggatc taaacgcatc acctctgggt aaaaactttt acccagcaaa
    19921 cccgctggcg ttccggcggc gtcgaagaat gtcatggcga ttttaaataa cccggcggca
    19981 gcgggtccta acacgatccc caccaacact gtgctgcacg agttacgcgc cgaccagatg
    20041 gagtgggcaa tgttggttga ccagacaaaa ctccacgcgc ctttaatgtg tcgggcagac
    20101 tcaaacagat tcaatttgaa ggcgttatgg atatttctac ggcgtaattc gcgcgcggca
    20161 aaccaccagt acatggtgcc gccaaccaga ttcgacacgt accaggcaat aacaaaaccc
    20221 gcaaaaccaa agtcaaaata ccaggctgcg acgctccccg ctgcgcgcag aaaaggtttc
    20281 gtcgcctgct gtacagcaat caaatcaaag cgatctaccg cacgcagaat gccggtcggc
    20341 gtggaggaag ccattgaagg aatgagcgtg caatacagcg ccgccagcca aaagctttgg
    20401 tcatccagac ctaatgaatg ggaaaggaat ggcagtaagg caatgccgcc gacaatcgcc
    20461 accgcgccgc tgacgatatc cagcgagaag gaaaatgaga cgacattgcg aaattgctgc
    20521 ggattattgt tggtcaaggc tggcgttccg tactgaacca ccagctgcca tgtctgaaac
    20581 ttaataaaat cgctgatcga cttggcgtac gattgcacaa tcaccagtac accaaacatg
    20641 gcgggcgtca tccctttacc ggcacacgag agcgccaaca gccccagcaa ggcgctctcg
    20701 acattactgg agcctaacca ggcgctattg cgaataatgg tgcgaaacgc gccatctgca
    20761 aaccaatgtt tgatgttaaa acccgccaat tcagcctgac cttatcgttg atagtaaaaa
    20821 atatcccgcc agccttaagt taaacttcgg cggtcagaaa cgatggcaac cagagaaacc
    20881 gccttctgta cctgttccag cacttcgctg tagggcgctc tggaatcaat ctcaagaatt
    20941 tttgtgccgt tatagccaat cgtcgacatg acgccgattt tgtcctgcag ctcggcatac
    21001 tcatggtcag gcttgcggga gatggcagta tcaatatcaa tccccaaacg aatgattaat
    21061 tcaggacggt attgcgccat tttttggtat aaactccgtt cgcgctgcgc cagaaacatg
    21121 ctgattttcc cggtcgcacg ttcgacgccg atccccggtc cgtcataata aaagcccgaa
    21181 atttcagcct gcgggaagcg atcgctgacc accagaacgc cactttgcgc cagtcgctga
    21241 accttgcgta gattcgccat tcttcgcagc gagaagcagt acataatcac cgccgcccat
    21301 agcgccggag atttggtttt catgctttgg gttttcgagg atttggccgc caatcgccgt
    21361 tccagccaga cgccaaccaa cggcaatcgt ttgattttgt cgccgtcttc gccggagagc
    21421 agccccagat agcggcgctc ggtttgccag tgttgttgca gcgatttcac caggtcggtg
    21481 gtcagcgtgg atttaccggt gccatcacac ccgaccaccg caatcagccc cggaatgtag
    21541 ttgggctgcg gcgcggttgt actattaaca gtttgtgttt gtagtgcatc cattcgttaa
    21601 atcatcccct atccattttg gtgatcagga aagcgcggcg tgaattgcgc gtagtgacgc
    21661 ttgcataatt tcatcggctg gttgccgacc atccagttca aggatcttgg cgccattgaa
    21721 tgtcagttgc ggcgtaacgg cgattttttc ctgtaacgct gccagttggt ggtcaggttt
    21781 acgcgcaaac gcggtttgtt catcaatgcc aagacgaatc aacaatacgg gtaaataaga
    21841 cgccatccat tggtacagct tcaactcgcg ctgccttaac atttttatcc aaccgttacc
    21901 gcccgtggtt tttgccaatt gcgggccatc aaagcgaaaa cccggcactt caacctgcgg
    21961 gtagcggtcg gtgatgagca gatagccttg ctggctttta cacaacattt tgcgaaactt
    22021 gtacgcccgc cagcaggaaa gcagatagat aaccagcgca gtaatattgc caggcggagt
    22081 taagggcttt tcgtgtacat gtgccgcttt acttcgcaga taacacccac aaggtgcgcc
    22141 aataacaggg agctgtgaaa tccattcgcc aattcgcccg gacgattgcc cgagataaat
    22201 gtgttctgtt ggcattcttg ctgccagttc atttaccagg cttgccgtga gggtcgattt
    22261 acctgaacca tcacatccta caatggcaat aacacgcact ggcgtggaat taattattga
    22321 catattacgt tgattcacga aaaacccggc agtaaataat gtattgaata ttacgtggtc
    22381 cgtgctcaga atatccgttc aaccttattg tgacaatggc taattgtgac attgacctga
    22441 attacgcttt cataaaaaca tattaaccaa ataaatattt ttaatggata tttaaattaa
    22501 aggatatatt catgcagtca ataacacctc cattaattgc cgttattggt agcgatggtt
    22561 caggcaagtc aacggtgtgt gaacatctta ttaccgttgt cgaaaaatat ggtgctgccg
    22621 aaagagttca tttaggaaaa caggccggaa atgtcggtcg tgcagtgaca aaattacctt
    22681 tgatgggaaa atccttacat aaaacaattg aacgaaatca ggtgaaaaca gcaaaaaaat
    22741 tgcctggacc agttccggcg ctggtaatta cagcgtttgt cgcccgtcgc ttactgcgct
    22801 ttcgtcatat gcttgcctgt cgtcgtcgcg ggttaattgt tctaaccgac cgttatcctc
    22861 aggaccaaat tcctggcgct tacgatggta cggtgttccc acctaacgtt gaaggtggtc
    22921 gttttgtctc atggctggca agccaggaac gtaaagcgtt tcactggatg gcgagccata
    22981 agcctgatct ggtcatcaaa ctcaatgttg accttgaagt tgcctgtgca cgtaaacccg
    23041 accataaacg ggaatcgctg gcgaggaaga ttgccataac gccacagtta acctttggtg
    23101 gtgcacaact ggttgatatc gatgccaatc agccactgga acaggtgttg gttgatgcag
    23161 aaaaagcgat tacggatttt atgaccgcgc gtggttatca ctagtcaaaa atggaaatgc
    23221 ccgatcgcca ggaccgggca ttttcaggaa ggttaaatca actgcaatgc tatccagtac
    23281 agcccaccag aaagaaaaat tgccgccggt aaagtaaaca cccacgccat caggatgctg
    23341 gttaccgttt tacgctgtaa cccaccgccg tccaccacca tcgtccctgc taccgcagac
    23401 gagaggacgt gtgttgtgga gacaggcatc ccaatataac tggcaaggcc gatagacact
    23461 gccgccgtca tttgtgccgc catgccttgc gcatacgtca tgccgcgctt accaatcttc
    23521 tcaccgatgg tcatcgctac acgacgccag ccaatcatgg tgccaatgcc gagcgccagt
    23581 gctaccgcca tgatgatcca caccggagcg tactcaatgg tgcttaacat atcgctgcgc
    23641 agttttttca gcaggttctg gtcttcttta ctgacgcctg gcagtttcgc cagcttcgcg
    23701 gaggtatcgg agatgcacag cataatgcgg cgcagctggc tgcgctgact cacgcttaac
    23761 ggctcgtaac tttccatatt gcctggcagc atcgttttaa cgcgcgcaat cgcatcaaag
    23821 gtatttgccg gatgacagtg aaactctgtt acttgcgtgc catcagtcga tgctgcaggc
    23881 aatggaggtt ccatcgcaat caacttctgc ggcagttcag gatgctgttg caggtagtgt
    23941 tcgaagttgg taacggcatc gcgggtacgg gtaatttcat agccggacgc attcatattg
    24001 acgacgaagc cagcaggggc aatccccacc agtaccagca ttaccaggcc gatccctttc
    24061 tgcccgtcgt tcgcgccgtg cgaaaacgcc acgcccgcag cggaaacaat cagcgcaata
    24121 cgcgtccaga atggcggttt acgtttgccc tttttctttt tgcgatcttc cggaatgcgg
    24181 tgaatacggt cacgcttttt cgtcccactc cagaaacggc gcagcaggaa aatcagacca
    24241 cccgcaatga ccaggccgac gataggggaa acaatcagcg aggagaaaat tttggttact
    24301 tcacgcaggt ttaacgcatc catcaccgat gagccggtta acagcgcgtt ggttaaaccg
    24361 atgccgataa tcgcaccaat caaggtgtgc gaactggagg ccggtaaacc aaagaaccac
    24421 gttcccaggt tccagataat cgccgccagc agcatggaaa agaccatcgc caaaccgtga
    24481 gttgacccca tattcagcaa caaatcgatt ggtaacatat ggacaatggc ataggcaacg
    24541 ctaagtccgc ccaataacac gccaaaaaag ttaaaaaatg ccgccatcac cacagcaagt
    24601 tgtggctgca tggcacgagt ataaataacg gttgccaccg cattcgccgt gtcatgaaaa
    24661 ccattgattg cttcgtagaa caacacaaat gccagagcaa gtaataacaa aagccccgta
    24721 tatatatcaa ggccaacaaa taaatttagc atatattaga ttacgccatt ttgaatttac
    24781 gaacggacgc attatcagtg actttaacgg catgggcaaa gtgaaatatc attttttaac
    24841 gataaaaaga gtgttatttc ttgtgtatca gacaataagc attcattcaa aacctttaga
    24901 aataatcaaa acacaggtat tccactggtt aatatattca catatgaaat gaataaatat
    24961 tggaatatat aaatattgaa tattttgatt aatccccgta ctaattattc ttcataatca
    25021 gtacggggtt gctacaacat gtattacttt ttcaccacaa ttaacggttc aatatcactc
    25081 tcttttttga tgaccagtga ttcatcaccg cgcaaacacg tcccaccgta gttgccgcca
    25141 acggtgaagg tacatacctg aatgtattta ccgtccactt tcggcaaaca ccacagttgc
    25201 tgatagatgt ttttctgctc ggcaaattta ccgctggttt tgtccagcac ctcttcatga
    25261 tggctgacca ggtcgatatt gctaccacag cgaccggcga tcggtttcac tgcgtaacct
    25321 gttttcacca attcatcatt aacagtgaaa tcggtatcca gcaggtaacg atggtgcggg
    25381 aacagcgacc agaggatcgg cagaatcgct ttgttgccgg ggatcaccgt ccacagcggc
    25441 tcaaagacca gcacttccgg gcgcagcaat acgtcgataa gacgcacttc gttttgcgga
    25501 tgaccggtac ggattggcac cgcagcaaac tcacggtcgc taacttcacg aatctgatca
    25561 aacgcggttt cccacgccca ggttttccac acgcagttaa ccagtcgccc ttccccatca
    25621 atcagttgcc cggcagcatc ccagcccagt tcatccagcc cgcgcaagat acgcgtttca
    25681 aagcccgcct ggtgcagcgc ctgctccata aactgcgcgt gatagttttc ctcgatatct
    25741 ttgtcctgca tgatatggac aaacggacgt gcacgactgt gtttccaggc accagccagt
    25801 tcgttaatca gcccttccgc cggattgaag ccgttgcctt tatagccctg ctccgcccaa
    25861 cgttcgagga tcaagcccgc ttcggtatga caggaggcgg agtcggcgtt gtattcgtaa
    25921 accttcaggc cacgctcatc catgcagaaa tccatacgac cagtgatcat atggtgacgg
    25981 cgacgctgcc aggagagacg caaacgtggc cagaggattt tcggaatgtc gaacagcgcc
    26041 agcaggttgt catctttcag caccttgtcg gttgcgtgaa gatacatcag atgcagctcg
    26101 ttggtggctt taattagctc ctgctcggca ctctcggtaa tggtgtagta atgatacgga
    26161 tcctgattga tcacctgacc gttggcctgc acataggcgt tttgtagcgg atctttttca
    26221 tccagccatt taccgtcaaa ctggcctttg ttttccagac gcgctccact gattttcagc
    26281 agctcgcctg caatttccgg ctgcggtaag ctgtattcag tatcttccgt ctggatcatc
    26341 cagcccagaa tggtggtgtc atcaaaagtg tctttaaggg tatagcagcc gttttcgacc
    26401 accatctcca gctcgcgcgt ccactgttgc ccttgcggca acggggaatg aatcacgttc
    26461 tgttccgcaa tacggacttt gttgccatgc aattgggtaa tgatggcgac atggccagtg
    26521 tctttaaatt caccgccttt atcccagata agaagcgcac ccgcgaccgg cgcacgcggc
    26581 gagccgttag gaaatgcctg caatggcagg atgttgtcat taaccacttc tcgcaggaag
    26641 cgcagcgaga aaatctccca cgccataccc acgtcagtaa agaccacacc gtaattcaga
    26701 aagagaaaac ggcgagcaaa ttcaacgcat tgccacttgt ggcccatata ttcgtcgtcg
    26761 atatagctac ggaatacggc gtcatcttcg tattcctgcg gatcgagaga actgtaatct
    26821 gaagagtaga ttgctacccc acctggggcg tagcccaata atgtcccgaa cggggcatcc
    26881 tggctggtcg ttcctttgct catcacttta cctttaacaa tacaacctaa gcagttggcg
    26941 taattgttgt gctctgatta cctgctcagc gacattaacc ggacagaggt cagactaatc
    27001 atacacctca tcgcagcggc tggcgcaaag ggttaaaaaa ttcacattct gtacagcaag
    27061 tgacctgcta cactgcttca acactaccac tcagaaggca actcactatg acagacaata
    27121 cttatcagcc cgcgaaagtc tggacgtggg ataaatccgc tggcggcgcg ttcgccaata
    27181 tcaatcgccc gttttctggt ccgacgcatg aaaaaacgct gcccgttggc aaacatccat
    27241 tgcaacttta ttcgctggga acgccgaacg gtcagaaagt aacgattatg cttgaggagc
    27301 tactggcgct gggcgttact ggtgcagagt acgacgcctg gctgattcgt attggcgatg
    27361 gcgatcaatt ctccagcggc tttgtcgaag tgaacccaaa ctcgaagatc ccggcgctgc
    27421 gcgatcatac gcataatccg ccgatccgcg tgtttgaatc tggttcgatc ctgctttatc
    27481 tggcggagaa atttggctac ttcctgccgc aggatctggc aaaacggact gaaacgttga
    27541 actggctgtt ctggttacag ggtgccgcgc cgttcctcgg cggtggtttt ggtcactttt
    27601 accattacgc gccggtgaaa attgagtacg ccatcaaccg ctttaccatg gaagccaagc
    27661 gcctgctcga cgtgctggat aagcaactgg cgcagcataa gtttgttgcg ggcgatgagt
    27721 acaccattgc ggatatggcg atttggccgt ggtttggcaa cgtggtgctc ggcggcgtgt
    27781 atgatgccgc agagtttctg gatgcgggaa gttataaaca tgtgcaacgc tgggcgaaag
    27841 aagtgggcga acgtccggca gtgaaacgtg ggcgtattgt taaccgcacc aacgggccgc
    27901 tgaacgagca gttgcatgag cgccatgacg ccagtgattt tgagacaaat acagaagata
    27961 agcgtcaggg gtaagggttg gtgttcgtcg cagcaagcca tccaggccgg ataaggcgtt
    28021 cacgccgcat ccggcaatcg tgcacaatgc ctgatgcgac gctgccgcgt cttatcaggc
    28081 ctacaaaagc taatcatcac gcgctggtaa tgtcgtaatc catttggcgc agtgcgtcta
    28141 atgtggcttt ggcttcatct tcgtcgatga tgctcatggc aaatccgacg tgtaccagca
    28201 cccactggcc cagcagatcg gcagggttac cttcgcaaat cagggcgata ttcacgtcgc
    28261 gcttgatacc acatacttca acctgcgcaa gctggtgaat atcttcaccg acagccagca
    28321 cctggcctgg aacgccaata cacattgtta actccggtta tttcgattgc cggacaaacg
    28381 cgaaaaagaa cctttagcgg gtgttccctc tcaccctaac cctctccccg gaggggcgag
    28441 gggaccgtcc gtgcgctttt taggcgttgt cctcagtctt attcaacttc aatacttttg
    28501 acgatcagcg aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc
    28561 gcatcgtgct gatgaatctc caccacctgg ctgcaatccc agcaccaggc ctgggcgggt
    28621 ttatagacga tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa
    28681 ctaaaacgga cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg
    28741 gtgacgcgct taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg
    28801 caaagcgaca actcatgcat tttcgctcct cctgcggcca aacagcaggg cgcgacgccc
    28861 tgcgtgtggt acatccggat tggtgactgg caacgaaagg atcattcgtg cgcagtcatc
    28921 cgtcaggcgt tggccttctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca
    28981 ggagaggtat tgcgaaacac cgtccagttc gccaacggta aaggtcatgg tgccatacgg
    29041 cagttgcaga ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg
    29101 gaagataact gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa
    29161 cagcgtaaaa tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat
    29221 cgaacgccgg gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa
    29281 acctgctatc tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc
    29341 gcagcgcagc cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta
    29401 agccgatgtg tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa
    29461 actcaccggt gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga
    29521 ttttgttggt aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggag
    29581 tgttcttttt cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc
    29641 caagcagctc cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt
    29701 atcgttgctc taacgcttcg acaatccgca cgccgatggc ttcatcggtc agcaaaatat
    29761 tgccgacccc taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc
    29821 gtcggcatcc actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg
    29881 caccacttcc agcggtttat tcggatcggc aaccggtgta cccaccagcg actgctcgta
    29941 aggaccgacg tcatcattga agttacgcgg accagagttc caggttgatg gaacaaccgc
    30001 ctggtagttg ctgatgatac cgtctttaat aaccatccag tgagagagca taccgcgcgg
    30061 cgcttcgagg aagccaacac ctttgaactc acccgttgcc ggaatgttcg gcttcacaaa
    30121 ggtggtgtga tcgcctttgc cgatattggt gatcagtgca ctgtattggt tttgcaggat
    30181 atcctgcaat tcacagcagt gaacggtacg accaataata cggcccagcg tggagtgcag
    30241 ctgtgccact tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag
    30301 tttgttctgg gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc
    30361 cacttctacc gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc
    30421 agaccaacca tcataagccg gaatggtggt accttcccac ggcgcctgtg gcgcttcgtc
    30481 tttataccag gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg
    30541 agaagtgatc ggacgatacg aggacagatc cgcattctca atgtagcctc ccgggaacag
    30601 gaagctgccg tttttgctgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc
    30661 tttaccgcgc gtcagccatt ccgggtagaa cgcggcgata accgcggtat caaccttata
    30721 aacctgctca acaaagtcgc tcagtttgtc gatgaaagac ttgatgtaca tcaggcgctc
    30781 aaggttcaac acgcccaacc cgtcgaggtt gattgggttc gcgacaccac ccactgccag
    30841 gttctgaatg tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg
    30901 gcactccagc gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt
    30961 catcgccggg tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag
    31021 atctttgatc ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga
    31081 aacgcctttc agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc
    31141 cacccagtcc agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc
    31201 agccagaatg atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact
    31261 ttctgccgca cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg
    31321 cacaatcatc catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca
    31381 catggtaccg gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg
    31441 taaatgcccc tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc
    31501 gcctcatgac gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg
    31561 agtacgatgt aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc
    31621 caggtcggga agtaggcgta accgccgccc gggttgaatg ccaccaacga ataggtcaga
    31681 cgccaggttg cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca
    31741 ttacgcagct tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc
    31801 cagaacatca cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata
    31861 agctcgccaa agcgcagccc gatgaaaatc gccagcaaca cactgatggt gttggtcagc
    31921 ttgacgaaca ggctcttttc atccggaccg ttgccacgca gacccgcctg caccagtgaa
    31981 ccttcaaaga tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc
    32041 aacatttcat agctctgcca gagcggatgc accttgtagc ccgccgagat catcagcgac
    32101 cccattgaag actggtgcat ggtcggcagc agcgcaccga gcgcgatgat gaagaacatc
    32161 accttgttca gacgttgtag cgacaccttc cagcccagac gttcaaacag tgccggagca
    32221 aactccagag ccatcacgcc gatgtagatg gtcatacaga ccgccgtctc gaacagtacc
    32281 gagttcacgt tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg
    32341 tcgatagtga tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga
    32401 cgcaccagcg gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag
    32461 ccgccacagg caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg
    32521 aagccgccgt tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg
    32581 agcatacaga tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg
    32641 cccagcggtt gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt
    32701 gttgcgacga accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt
    32761 atacagggtg tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg
    32821 caggtcgaga ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc
    32881 tttctcgccg tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga
    32941 tttcagcgtc tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt
    33001 cgcctccgcc atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc
    33061 tacgcagcca ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc
    33121 gcacttatgc agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca
    33181 ggcgaccatg cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt
    33241 tttcggatct tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca
    33301 gtgcatacac tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc
    33361 tgtgccgctg gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt
    33421 cgaccaggtc tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt
    33481 gacgcaagcc tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg
    33541 aattggcggg cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa
    33601 tgccccgcag gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag
    33661 tcagcgttat ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg
    33721 gcaaccagcc caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc
    33781 tctttagcgt taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc
    33841 tgatggatgc ctttatggaa gccgatacct ttttcgttac agccatagca cgggtgacca
    33901 atcgccaccg gccacacacc gccaacatcg cagaattgca gcgttgagca gttgccgtaa
    33961 gtttctggcc ctttacagcc gaggtggtac aggcaccagc cttcgcggtg gccttcatca
    34021 ccgaactctt tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga
    34081 atcagacggc cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg
    34141 taagtgatga tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga
    34201 atattgataa cggttttgcc tggcagaact tcttgcaagc tgactgcgcc agttgggtta
    34261 actccagctg cggcaacacc gccccacgcg gagcaggaac caatagcgat aatggctgct
    34321 gagccttccg ccgctttgcg gatgtgatcc acaatcggct caccggcaac catgcaataa
    34381 ataccgttat cttttaatgg gatggaacca tccaccacta acacatactg ccctttgtac
    34441 ttctcgagag cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact
    34501 tcgtgatatt ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca
    34561 cgaagcagag attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga
    34621 cgctgcgggt tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg
    34681 gtggcggcta atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg
    34741 atgagggtgt tatctccagt catttatagt tattccgttg cgaggacctg gcatatattt
    34801 tgcctcaatc gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg
    34861 ttatcgatat catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa
    34921 ttaattaaag caagataata cgtatgtttg atcaattttc gaggcaataa ccggccataa
    34981 aaaagcgcaa cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat
    35041 ccagtccata gcggcgcgtc agaatacccg cttcccaggc gctaagctgg cgatctccgg
    35101 tcttttcgta catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga
    35161 attttgtcac attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt
    35221 taccaaaatg gttattcatc gttcacctca ttcgttatat atctccatat atagcgatat
    35281 tttaggcaaa gaacgacttt tgcgcacgct attttttcat acttgtttaa gacgtaatcc
    35341 cgtaagagat aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag
    35401 gcaagatcgg cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta
    35461 acccccggat agatataagc ctcataaacc ttattattgt ctttcaacgc cgcctcgtaa
    35521 ggaggccagc cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct
    35581 tcaatcttcg ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc
    35641 agttccggat acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta
    35701 atacccactt tacctgtcgc ttgcggatag cgttgcataa actcaattgc ggcaaagaaa
    35761 tcattcatca gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt
    35821 cccggataac ctccaacgga acttaatccg tcaggtgcca gggcgatata ccccgccttc
    35881 gccactcgcc gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc
    35941 cccacggctg gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg
    36001 ccgtgaccat ttggcgaagg atacgtgatg tactcagcaa caatctccgg gtcggtaaac
    36061 tctacctgag tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc
    36121 atcccgccca ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg
    36181 tgaacgtaat agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc
    36241 ggcatcgttg cattcctcaa ctggtgtttt tttaagcaaa gcataagcgc gtgtttttgc
    36301 ccagtttttc gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact
    36361 agaaacattg tttcgaaatt gaacggagga aaggagaggt catggtctgg ttagcgaatc
    36421 ccgaacgtta cgggcagatg cagtaccgct attgcggaaa aagtggttta cgcctgcccg
    36481 cattatcgct cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg
    36541 caatcctgcg taaagcgttt gatttaggca ttacgcactt tgatttagcc aacaattacg
    36601 ggccgcctcc aggaagcgca gaagagaact ttggtcgcct gctgcgcgag gattttgctg
    36661 cttatcgcga tgaactgatt atctctacca aggctggcta cgatatgtgg cccggcccgt
    36721 acggctctgg cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta
    36781 tggggcttga gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg
    36841 aagaaaccgc ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga
    36901 tctcctctta ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa
    36961 ttccgctttt aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg
    37021 gcctgctgga taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc
    37081 agggattgct gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg
    37141 aagggaataa agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc
    37201 tgcgcttatt gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa
    37261 gctggttgct gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc
    37321 aactagagga gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc
    37381 agattgatca gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat
    37441 gacctgttaa taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc
    37501 agtcggtcaa gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg
    37561 taccacatca gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga
    37621 atggaagaaa ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt
    37681 ttccccagcc acttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc
    37741 tcataaccgg aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc
    37801 gacagcaaca cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga
    37861 ataatctcct ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac
    37921 acgggggcaa gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat
    37981 tcctgtcgtg tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca
    38041 gcaaatcaac atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg
    38101 gttcccttat caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga
    38161 aaaacgatgt ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa
    38221 cagaaacaac cgacgccagg catccaggcg aagatgacac cgataccgga ttgcggcgag
    38281 aaaacctatg ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat
    38341 tccggaatag gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc
    38401 agttatcttc ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc
    38461 ggacgcaaag ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg
    38521 gttcacgaag cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa
    38581 caggttgcca ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc
    38641 attaacgttt tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt
    38701 gcaagtgtca tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac
    38761 tatgcggcta cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg
    38821 gagaaaggta ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt
    38881 tccggcggac aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt
    38941 gcggggcaac cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc
    39001 tacgtcaccg cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc
    39061 cggttgtgaa aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac
    39121 gccttatccg cctacaaacg catgaagatt caacatgttg caatagattt tgtaggcctg
    39181 ataagcgtag cgcatcaggc attgagcacc taatgcctga tgcgtcgtga acgccttatc
    39241 cggcctacaa acgcacgaag attcaacatg ttgcaataga ttttgtaggc ctgatgagcg
    39301 tagcgcatca ggcaattcta ctttacttcg ctttggcggt ttcttcgccg accagaccta
    39361 tcttcaggta acccgcctga tgcagcgtat ccattacctt catcaacgtc tcgtaatcga
    39421 cggttttgtc cgctcggaag aagatggtgg tgtctttctt gccttcggtt aacgcattca
    39481 acgccgtaat cattgtttca tcggtgaccg gatcgttacc gataaacatc gagttgtctg
    39541 ccttcaccga cagataaacc ggtttttccg ggcgcggctg cggcgtgctg gtagaagcag
    39601 gcaagttcac cttcacatct accgtcgcta acggtgccgc caccataaag ataatcagca
    39661 gaaccaacat cacgtcgata aacggcgtca cgttgatatc atgcatttcg ccgttatcgt
    39721 cgaggttttc gttaagatgc attgccatcg gacattatcc tgcgcgtaat ttttgtgcga
    39781 cacgaaccgg atgcgcagcg gcgctggctt ccagatccag gtcacggctt tgcagcaaca
    39841 atacctgcgc tgcaacatca cccagcatcg ctttaaagcc gccaatctgg cgtgcaaata
    39901 cgttatagat aacgaccgca ggaatcgctg ccacgaggcc gattgccgtt gctaacagag
    39961 cttctgcgat acccggcgca acgactgcca ggttagtggt ctgcgtttgc gcgataccaa
    40021 taaagctgtt catgatgccc cagaccgtac caaacagacc aacgaacgga gaaatcgcgc
    40081 cgatggttgc cagatagccg ttaccgcggc ccatttgacg acccactgcg gcgacccgac
    40141 gctccagacg gaagctggta cgttctttaa taccttcgtt atcgtcgctg ccttctgaca
    40201 gttccagctc attctgcgct tcattgagca aatgcaggct taagcttttg ctaccaaaat
    40261 cagcggcgat atcgttggcc tggtttaagg aacgtgcttc agccagcagt tgctgctcgc
    40321 gcttaaggcg acgcttctga ttgaagaact ctacgctctt actgaagaag attgcccagg
    40381 tgactacaga ggccaaaata agcccaatca tcacgcactt aacgacaata tcggcgtact
    40441 gatacatacc ccagacggaa aggtccgtct gcattaaatt attacccact gtgtatctcc
    40501 aggacgcaag tcacaaaatc tgcgcataat aatatcaaaa cgacgtcgaa ttgatagtcg
    40561 ttctcattac tatttgcata ctgccgtacc tttgctttct tttccttgcg tttacgcagt
    40621 aaaaaagtca ccagcacgcc atttgcgaaa attttctgct ttatgccaat tcttcaggat
    40681 gcgcccgcga atattcatgc tagtttagac atccagacgt ataaaaacag gaatcccgac
    40741 atggcggaca aaaagcttga tactcaactg gtgaatgcag gacgcagcaa aaaatacact
    40801 cttggcgcgg taaatagcgt gattcagcgc gcttcttcgc tggtatttga gagtatggaa
    40861 gccaaaaagc acgcgacgcg caatcgcgcc aatggcgagt tgttctatgg acggcgcgga
    40921 acgttaaccc atttttcctt acaacaagcg atgtgtgaac tggaaggtgg cgcaggctgc
    40981 gcgctatttc cctgcggggc ggcagcggtt gctaattcga ttcttgcttt tgtcgaacag
    41041 ggcgatcatg tgttgatgac caacaccgcc tatgaaccga gtcaggattt ctgtagcaaa
    41101 atcctcagca aactgggcgt aacgacatcg tggtttgatc cgctgattgg tgccgatatc
    41161 gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg aatcgccggg ttccatcact
    41221 atggaagtcc acgacgttcc ggcgattgtt gccgccgtgc gcagtgtggt gccggatgcc
    41281 atcattatga tcgacaacac ctgggcagcc ggtgtgctgt ttaaggcgct ggattttggc
    41341 atcgatgttt ctattcaggc cgccaccaaa tatctggttg ggcattcaga tgcgatgatt
    41401 ggcactgccg tgtgcaatgc ccgttgctgg gagcagctac gggaaaatgc ctatctgatg
    41461 ggccagatgg tcgatgccga taccgcctat ataaccagcc gtggcctgcg cacattaggt
    41521 gtgcgtttgc gtcaacatca tgaaagcagt ctgaaagtgg ccgaatggct ggcagaacat
    41581 ccgcaagttg cgcgagttaa ccaccctgcc ctgcctggca gtaaaggaca cgaattctgg
    41641 aaacgagact ttacaggcag cagcgggcta ttttcctttg tgcttaagaa aaaactcaat
    41701 gatgaagagc tggcgaacta tctggataac ttcagtttat tcagcatggc ctactcgtgg
    41761 ggcgggtatg aatcgttgat cctggcaaat caaccagaac atatcgccgc cattcgccca
    41821 caaggcgaga tcgattttag cgggaccttg attcgcctgc atattggtct ggaagatgtc
    41881 gacgatctga ttgccgatct ggacgccggt tttgcacgga ttgtataaca ttgccacttt
    41941 tggacaattt tgcagacatt ttattgtgaa aagtcttaaa ttgttgcgtc cgggatcaag
    42001 gcgtcccgga cgattcagga gtacaatagg cagataaagg cttaaacgct gttccacagg
    42061 aaagtccatg gctgttattc aagatatcat cgctgcgctc tggcaacacg actttgccgc
    42121 gctggcggat cctcatattg ttagcgttgt ttactttgtc atgtttgcca cgctgttttt
    42181 agaaaacggc ctgctgcccg cctcattttt gccaggcgac agcttgttga tactggcagg
    42241 cgcattgatt gcccaagggg ttatggattt tctgcctacg attgcgattc tgaccgccgc
    42301 agcaagtctg ggctgctggc taagttatat tcaggggagc tggttaggga ataccaaaac
    42361 ggtgaaaggc tggctggcac agcttcctgc taaatatcac cagcgcgcca cctgcatgtt
    42421 tgaccgccac ggtctgctgg cgctgctggc tggacgtttt cttgcatttg tccgtacgct
    42481 gctgccaacc atggcgggaa tttccggtct gccaaaccgc cgcttccagt ttttcaactg
    42541 gttaagtgga ttgctgtggg tcagcgtggt aaccagtttt ggctatgcct taagtatgat
    42601 tccgttcgtt aaacgccatg aagatcaggt aatgacgttc ctgatgatcc tgccaattgc
    42661 cttgttaacc gctggcttgt taggcacgct gtttgtggtg attaaaaaaa aatactgtaa
    42721 cgcctgacga ttttccccgt tcccggctgc tgtaccggga acgtatttaa ttcccctgca
    42781 tcgcccgcat tcttgccgca tcttcccccg gcgtcacacc gaagtaacgt ttaaactcac
    42841 ggctaaattg cgatgcgctt tcatagccga cgcgcatcgc tgctgcgctg gccttcatgc
    42901 cgtcatggat gatcatcatc cgcgccttat gcagacggta attcttcaaa tactgcaacg
    42961 gcgaggtgct ggtgacagac ttaaaattat ggtggaacgc cgatacgctc atgttggctt
    43021 ctgccgccag ttgctcgacg ctcaggtttt cggtgtattt attctcaatt cgtttcagca
    43081 cgcggctaat cagactgaag tgagtctggc gactgaccag cgccagtaac gcgccgccgc
    43141 aaggtccggt cagcacgtag tacagaattt cgcggatgat ctgtttgccg agaatacgcg
    43201 catccagtgg tcgctccatc acgtcgagta accgctccgc cgcgcataaa atctcttctg
    43261 ataacgtggc ggagttaatc ccgctggctg ccatcgacgg ctggaaatgc tcatcttcgc
    43321 caatgtccat caacagttcc tgtaactgca aaatatcgac attgagacgc aaccctgcca
    43381 gcggcacctc tgacgtggca taggtttcgc actcaaacgg caacggcacc gtcagcagca
    43441 ggtattcatt ggcatcataa cgaaacacgc gttcattgat ataaccgatt ttatgcccgg
    43501 aaaagagaat tatgatgcca ggctcgtaca tcaccggtgt acgtgcgaaa ggcgtctcgc
    43561 catacaacaa acgcacatcg ggcaacagtt ctgacaaact attttcttta tttttcagtt
    43621 tattaacttt atccgccagc aagcgacaaa tctcttcacg tttcatatcg cgtaatttct
    43681 taggaataat gcggcaattt gattgtgcgc aattttgtag catttctcca gcactctgga
    43741 gaaataggca agacattggc agaaatgagc attgagagcc agggcgctgg cgatcacaat
    43801 gaaaaacatc aggcagatcg ttctctgccc tcatattggc ccagcaaagg gagcaagtaa
    43861 tgaacaactt taatctgcac accccaaccc gcattctgtt tggtaaaggc gcaatcgctg
    43921 gtttacgcga acaaattcct cacgatgctc gcgtattgat tacctacggt ggcggcagcg
    43981 tgaaaaaaac cggcgttctc gatcaagttc tggatgccct gaaaggcatg gacgtgctgg
    44041 aatttggcgg tattgagcca aacccggctt atgaaacgct gatgaacgcc gtgaaactgg
    44101 ttcgcgaaca gaaagtgact ttcctgctgg cggttggcgg cggttctgta ctggacggca
    44161 ccaaatttat cgccgcagcg gctaactatc cggaaaatat cgatccgtgg cacattctgc
    44221 aaacgggcgg taaagagatt aaaagcgcca tcccgatggg ctgtgtgctg acgctgccag
    44281 caaccggttc agaatccaac gcaggcgcgg tgatctcccg taaaaccaca ggcgacaagc
    44341 aggcgttcca ttctgcacat gttcagccgg tatttgccgt gctcgatccg gtttatacct
    44401 acaccctgcc gccgcgtcag gtggctaacg gcgtagtgga cgcctttgta cacaccgtgg
    44461 aacagtatgt taccaaaccg gttgatgcca aaattcagga ccgtttcgca gaaggcattt
    44521 tgctgacgct aatcgaagat ggtccgaaag ccctgaaaga gccagaaaac tacgatgtgc
    44581 gcgccaacgt catgtgggcg gcgactcagg cgctgaacgg tttgattggc actggcgtac
    44641 cgcaggactg ggcaacgcat atgctgggcc acgaactgac tgcgatgcac ggtctggatc
    44701 acgcgcaaac actggctatc gtcctgcctg cactgtggaa tgaaaaacgc gagaccaagc
    44761 gcgctaagct gctgcaatat gctgaacgcg tctggaacat cactgaaggt tccgacgatg
    44821 agcgtattga cgccgcgatt gccgcaaccc gcaatttctt tgagcaatta ggcgtgccga
    44881 cccacctctc cgactacggt ctggacggca gctccatccc ggctttgctg aaaaaactgg
    44941 aagagcacgg catgacccaa ctgggcgaaa atcatgacat tacgttggat gtcagccgcc
    45001 gtatatacga agccgcccgc taagcttttt acgcctcaaa ctttcgtttt cgggcatttc
    45061 gtccagactt aagttcacaa cacctcaccg gagcctgctc cggtgagttc atataaagga
    45121 ggaacgtatg gctaatccaa ccgttattaa gctacaggat ggcaatgtca tgccccagct
    45181 gggactgggc gtctggcaag caagtaatga ggaagtaatc accgccattc aaaaagcgtt
    45241 agaagtgggt tatcgctcga ttgataccgc cgcggcctac aagaacgaag aaggtgtcgg
    45301 caaagccctg aaaaatgcct cagtcaacag agaagaactg ttcatcacca ctaagctgtg
    45361 gaacgacgac cacaagcgcc cccgcgaagc cctgctcgac agcctgaaaa aactccagct
    45421 tgattatatc gacctctact taatgcactg gcccgttccc gctatcgacc attatgtcga
    45481 agcatggaaa ggcatgatcg aattgcaaaa agagggatta atcaaaagca tcggcgtgtg
    45541 caacttccag atccatcacc tgcaacgcct gattgatgaa actggcgtga cgcctgtgat
    45601 aaaccagatc gaacttcatc cgctgatgca acaacgccag ctacacgcct ggaacgcgac
    45661 acacaaaatc cagaccgaat cctggagccc attagcgcaa ggagggaaag gcgttttcga
    45721 tcagaaagtc attcgcgatc tggcagataa atacggcaaa accccggcgc agattgttat
    45781 ccgctggcat ctggatagcg gcctggtggt gatcccgaaa tcggtcacac cttcacgtat
    45841 tgccgaaaac tttgatgtct gggatttccg tctcgacaaa gacgaactcg gcaaaattgc
    45901 aaaactcgat cagggcaagc gtctcggtcc cgatcctgac cagttcggcg gctaacgtgc
    45961 aaattctccc ggtggcggta atgttccgct accggacttt tcagaaatca tttattcccc
    46021 tcgcgtcccg cccgttgtta ctcttccttg ttcaggaatg ccaaatataa ggacatcatc
    46081 atgcagagcc ggaagctctt aaaagaacaa ctcatctata tccgggataa acgcaacgga
    46141 gaggtgaaaa acagatgaaa ataatacttc tgtttttagc agccctggca agttttaccg
    46201 tacacgcgca gcctccttca cagaccgtag aacaaacagt ccggcagatt tatcagaact
    46261 ataaatcaga tgccagtacg ccttatttcg gtgaaaccgg agagcgggcg ataacttcag
    46321 cgcgtattca acaggcgctt accctgaacg acaatcttac actaccgggc aatattggct
    46381 ggctggatta tgatccggtt tgtgattgtc aggattttgg cgatctggtg ttagaaagcg
    46441 tagcgataac ccaaactgac gtcgatcatg ccgatgccgt tgtgcgcttt cgtatcttta
    46501 aagatgataa agaaaagacc acacagacac tgaaaatggt ggcggaaaat ggtcgttggg
    46561 tcattgatga tattgtcagc aatcatggca gcgtcttaca agcagttaat agcgagaatg
    46621 aaaaaacgct ggccgcttta gcttcattgc agaaagaaca gccagaatcc tttgttgctg
    46681 aactctttga acatattgct gattatagct ggccgtggac gtgggtggtt tccgactctt
    46741 accgccaggc ggttaatgcc ttctataaaa ccaccttcaa gacggccaat aatcccgatg
    46801 aagatatgca aatagaacgg caatttattt acgacaatcc gatctgtttt ggcgaagagt
    46861 cgctattttc acgcgttgat gaaattcgag tcctggagaa aaccgccgat tccgcccgca
    46921 ttcatgttcg ttttacgctg accaatggca acaatgaaga gcaagagctg gttttacagc
    46981 ggcgcgaagg taagtgggaa atcgctgatt ttatccgccc gaacagcggc agcctactta
    47041 agcagattga ggcaaaaacc gccgccagat taaagcaatg agctgaatta aataacaatt
    47101 agccggaaca ataaataaaa gggaacacta tatgaaaacg attttcaccg tgggagctgt
    47161 tgttctggca acctgcttgc tcagtggctg cgtcaatgag caaaaggtca atcaactggc
    47221 gagcaatgta caaacattaa atgccaaaat cgcccggctt gagcaggata tgaaagcact
    47281 acgcccacaa atctatgctg ccaaatccga agctaacaga gccaatacgc gtcttgatgc
    47341 acaggactat tttgattgcc tgcgctgctt gcgtatgtac gcagaatgat aaaaaaatcc
    47401 ccggcagcat gtcagttgcc ggggattttt tttaacgtcc aaccgccgct ttagggcgtt
    47461 tcttcgcacc agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag
    47521 gcgtctgacg ctgagtcgcc atcggcgtat gtttagtcaa cgccggacgg gtattgcggt
    47581 tctggcgacg agcttcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac
    47641 ggctgccaat cagatgcttt ttgcccatcg cttccagcgc ctgacggatt aacggccagt
    47701 ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg acgctgtttg tcgcccttcg
    47761 gtacgaagac gccttcgctc ttataaccaa tcttcgccag cggttttttc ccggtgtaat
    47821 acatggtggt tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg tcgagacgga
    47881 aacgatgctt tttcagccac agcgccaaat tcaccatatc ttcatcacgc gtaccggggt
    47941 gcgcagagat gaaatacggg atcagatact gctctttacc tgcctgtttc gagtaggtat
    48001 cgaacagctc tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc ttcgacaacg
    48061 gcccttcttc ggtatgttcc ggagcaatct tcagataacc gccgacgtga tgggtcgcca
    48121 actctttgat atagcgcgga tcttccacgg cgatgtcata acgtacgcct gaggcgataa
    48181 ggattttttt aatgcctttc agatcacgcg cacggcgata gaggttgatc gtcggttcgt
    48241 ggttagtgtc catgtgcgga caaatatccg gataaacgca cgacaaacgg cgacaagttt
    48301 gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat
    48361 cggaaatcac gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga
    48421 tcgaatcttc cgaacggctc tgaataatgc gcccttcgtg ctcggtaata gaacagaaag
    48481 aacagccgcc aaagcagccg cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg
    48541 gaatacgggc attgccatag gccggatgtg gcacgcgctt gtacggcagc gcaaaaacgc
    48601 tgtccatctc ttcggtagaa agcgggatag caggcgggtt gatccacaca tagcggtcac
    48661 cgtgtttttg catcaatgcg cgggcgcagc ctgggttggt ttcgtggtgc agaatgcgcg
    48721 aagcatgggc gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaatacgt
    48781 aggttttttc ccacggtttc tggcgcggtg gctgcacgat tacagctttc gcttcctgct
    48841 ttttcggcgc aacaggtttg ttatccgcac acggcaaatc ttcgccatac ggatgcggga
    48901 ttggggcgat tttccctggg gtatcaagac gggtggaatc cacaccgctc cagccaggca
    48961 gcgcctcttt tacgataatt gcggtattac gcacatcgcg gatttcacta atcggctcgc
    49021 ccatcgccag acgatgcact acttccacca gcggacgttc accgttacca aacatcagca
    49081 tgtcggcttt cgaatctacc agaacggaac ggcgcacggt atcggaccag taatcataat
    49141 gcgcggtacg gcgcaggcta gcctcaatac cgccgaggat caccggcaca tctttccacg
    49201 cctctttaca acgctgggta taaaccagcg tggcgcgatc cggtcgctta cccgcgacat
    49261 tatctggcgt gtaggcatca tcatgacgta aacggcgatc ggcggtatag cggttgatca
    49321 tcgaatccat gttgccagca gtaacaccaa aaaacagatt cggtttaccc agacgcataa
    49381 agtcgtcttt gctgctccag tccggctggg cgatgatccc gacgcgaaag ccctgcgctt
    49441 ccagcatacg accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag
    49501 taaccaaaat gatgtcgcag ctatcccagc caagttgatc catctcttca cgagacatcg
    49561 gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc
    49621 gatccggttg gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat
    49681 caaaggccgg ggattataag ccggaacgaa agagaaatcg aaaggtattc catactcgcc
    49741 ctcctcgggc gagtatgaag attacggtac cggattgacc aacagttgcc caatcgagcc
    49801 acggtccgcc atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg
    49861 ctgaccgaaa taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc
    49921 tctgtcttcc gggaacggca tcgcgccatt cacgttacgg atctggaaca ttacgccttc
    49981 aatatggaac gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg
    50041 cgcggtgaca tcaatacggt tgacgtccca cagctgtcca ttaatacccg ggtcatcacc
    50101 cagactgata tcgcgactgc gaattggcga accagccatg atttcagttg gcagcaagcg
    50161 catcggaaga ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccaa
    50221 ggtagaaacc agaatactgg atggctcaaa gaagccgcga atacgatcaa caatgctcgc
    50281 cgcttcacca caggtgatcg acacttcatc gccgttgctc atatccacca gaatctcgcg
    50341 gcgctcgccc ggtgccagcg aaagttgctt caccgataca ggagcaggca ggaatccctg
    50401 atcgccagaa atcacatgta acgggcgacc atcgctcatc tgtagttgat agcgacgaga
    50461 gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct
    50521 ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta
    50581 ttctggcgta ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac
    50641 accataatgg ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc
    50701 ggcaaggccg ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag
    50761 agtagctgcg ttctggcgaa taggcagtac gggtgcccag tcagcgtttg gcgacatcat
    50821 ccgtgccgga ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac
    50881 attttctgtc aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat
    50941 agtcggcccc aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa
    51001 tgaccagtgc gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagtag
    51061 cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc
    51121 tgcacaaagt gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc
    51181 tccatgtaaa actggctaaa gtaataccgt gcaggaaagt gttccccgca aaaatacaga
    51241 ttgccgactt aaacttttcc ggcggcttcg cgttctgcga cttctttatc gagctcggcg
    51301 attttttgtt ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca
    51361 tactgactga cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga
    51421 ttaagattaa tcttattcga agttgtagag acgcacacgg gaataatcgg gacgcccgcc
    51481 gcaattgccg cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc acggctgcgg
    51541 gttccttccg ggaacatcca gatggaaata cggcgttttt tgaaatgatt cacgacttcc
    51601 gcaatggtgc cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac
    51661 cagtacaact gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc
    51721 ggcggttgca caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata
    51781 tagatagcat tgccgtagtt ttcagcatct gcaggtttgc ggcactcaac tttcaggccg
    51841 aacagtggcg caagacggcc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc
    51901 gggctgaaaa ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta
    51961 ataataagac gaaagatata tagcataaca ccctctgaag gtctgagaga ctggcattgt
    52021 acgtcacctg aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt
    52081 taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg
    52141 aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgctag cgggaggaaa
    52201 cagcgccctc cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt
    52261 cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt
    52321 cgccagtgac tttctgtagc tcttccggac gcagtttaat tttgcgtttc ccaacatgaa
    52381 tggtcagcgt gctttgcggc ggcagaacgt acagttgcgc cagaccatcc tcgccacgcg
    52441 cggcttctgc cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca
    52501 gatcacttac cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg
    52561 aagcatcttc aatcaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag
    52621 ccttacccgc acggttacgc gctaccagat cgttaaaggt gcagacgaaa ccgtaacccg
    52681 catcggaagc catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg
    52741 ccccaggcgg caacgttaat ttgccggtga gtggttcgcc ctgaccacgc gccgacggca
    52801 gcgtaatcgg gtcgatggca tagctacgac cggtggaatc aacaaacact accggttggt
    52861 tactcttacc tttcaccgcc gctttgaagc tatcacccgc tttataattc aggcccggcg
    52921 cgtcgatatc atggccttta gcgctgcgta cccagcccat ctgcgacagc acaatggtga
    52981 caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcctgca
    53041 acggcgaacg acgatcgtca ccgtaggctt gcgcgtctgc ctgcagttct ttcttcagca
    53101 ggttattcat tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca
    53161 gctcactctg ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca
    53221 gttcgaggat cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt
    53281 tcggttcatc ttcgttacga atgatctcaa tcacttcgtc gatgttgaga aacgccacca
    53341 gcaaaccttc caggatatgc aggcgcttga ggactttctc cagacgatag ttcagtcggc
    53401 ggcgcacggt atcgcgacgg aacaccagcc attcggagag gatttccagc aggtttttca
    53461 ccgccggacg accatccaga ccgatcatat tgaggttaat gcgatagctc ttttccagat
    53521 cggtggtagc gaagaggtgg ttcatcacct gatccatatc cacgcggttg gaacgcggca
    53581 caatcaccag acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg
    53641 gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct
    53701 gatgcggtaa tgcgctgata accaccgcgc catcttcttt cttccacacc gcgcgcatac
    53761 gcactgaacc acgtccgttc tcgtagattt tacgaatctc ggcgcgcgaa gtgataattt
    53821 ccgcttcagt cggataatcc ggcccctgca cgatatccag cagctgatcg agcgtagttt
    53881 tcggctggtc gattaatgcg attgccgcct gagctacttc acgcaggtta tgcggtggaa
    53941 tatcggtcgc catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac
    54001 gggcaggtag cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg
    54061 tcccctgccc cagctcgctc aatagcagct cggaatattt cgacaaccgg gattcggtgt
    54121 aacgcattgc cgcgaacgat ttcggatcgt ccggcgcgcc ccagttcccc tgaccatcaa
    54181 ccagcggata acggtaagag aacggctgcg ccatcaggac catcgcttca taacaggcgc
    54241 tatcgccgtg cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa
    54301 atttggcgct ggcattcagg cccagttcag acatcgcata cacaatgcgg cgctgaacag
    54361 gtttcagacc atcaccaata aacggcaacg cacggtccat gatcacgtac atggagtagt
    54421 ttaagtaggc gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta
    54481 attctgattc ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac
    54541 attatctgcc gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg
    54601 acggcgataa aggcatcaat gtgccttgat atacaactct ttgctgtaga cttgatcggc
    54661 atagagggca gcacaaggtt gcgcccatgc cacaccactc ggcatggggg tccgctccgg
    54721 gcgattcgag aagttgaggt tatgcgagtc cagggaagcc taaagcatca aagtagtcga
    54781 ctgtaagtat acggttcagc ccagtttagc tgtgtctcca ccagtgcccc gcgcctcctg
    54841 ccataagtgc ggcttgctca tggatatccc cgagacggct tacggcgcac cggataataa
    54901 atttcgcgta tacatggtat tccttaccgg agaaattata agcccaacaa caatacgcct
    54961 gtgacattgt tcgctcaaac ttatcgcgag tgatttggct cacactccgg taaagattcc
    55021 ccatcacgcc aacggcacat aaacatccgt ttccagtcgc tgctctgtca ccccttcggc
    55081 aagattggtg tagtgaaaca gaatcggtgc tttacgcatt ttctcgccgc ttgcaggcag
    55141 ccagtggcga ataatgcccc atatcgtgtg tgaaatatcg tccagctcgc caacgtggcg
    55201 ggccacggca taacgtccac cggtaagttc accattgctc acaccataac gattatcggg
    55261 aatcggttcg ctaacgctgc cgcagatatc aaagcgaaac gcttccggtg cggtggtggc
    55321 aggggcgtcc caggcgacgc caaaagtctg gctttggtta acaggagaaa gccccgtctc
    55381 tttacgccac atgataaatt tcgccacact gtagttgagc aactcagggc tacagcggtg
    55441 cggcaacatc gccacaggga ttgatgggaa atcgataatg tttacatcca gtgtcaggtt
    55501 tgtcattttg cctccctgcg aaattattcc cgggaacgcg tcccgggagc ggtaacaatt
    55561 acggatttac tttgcggatc tgtttgacgt caatttcaac agaattccag tctttatcga
    55621 cttcaccctg aatctcaacc gtatctttcg gcgtcaccgt cacaccgttc cagcgtttgt
    55681 gatcgatatc aacattgata gtaccgctgg catctttaaa cacgtagaga tcgtcagaga
    55741 tgcgttcaac gatattgccg cgcagggtta cccaggtgtc gtcacgcagg gattttgcgc
    55801 tttctacagt cgttacgctg ccgttcggcc cctgaaatcc tccggcctga ctttgcgtta
    55861 ccgatgggcc agaaaaaccg ccctgctctg ctgccatcac cggtgcactg cacagggcca
    55921 ttactgcgat tactgctgcg aattttttca tgtttattac tccctttaat gtctgtttcc
    55981 gagcatttaa caagatagtc cttaacaact tcttaaggga aaaaaataaa atttaatgct
    56041 gtacagagcg cgttacaaca cggtttactg gcagcaaata cggttatcgc agggatgaaa
    56101 aaatgcgaat tttactgata gaagatgaca tgctgattgg tgacggcatc aaaactggcc
    56161 ttagtaaaat gggttttagt gtcgactggt ttacacaagg tcgtcaggga aaagaggcgc
    56221 tttatagcgc accttatgat gcggtgatcc tggatttaac cttaccaggc atggatggtc
    56281 gcgatatttt gcgcgaatgg cgagagaaag gtcagcgtga gccggtactg aacctgaccg
    56341 cgcgtgatgc gttagcggaa cgtgtagaag ggctgcgtct gggagctgac gattatctgt
    56401 gtaaaccttt tgcgttgata gaagtcgccg ccaagctgga ggctctgatg cgccgaacca
    56461 acggccaggc cagcaacgag ctgcgccacg gtaacgtcat gctcgacccc ggcaaacgta
    56521 tcgccacgct ggctggcgaa cccttaacac tgaaaccaaa agaatttgcc ctgctggaat
    56581 tactgatgcg taacgctggt cgggtactgc cgcgcaaact gattgaagag aaactgtata
    56641 cctgggacga agaggtcacc agtaatgccg ttgaagtgca tgtgcatcat ctgcgacgca
    56701 aactcggtag tgattttatt cgtaccgtgc atggtattgg ctacacatta ggtgagaaat
    56761 gaaatttacc caacgtctta gtctgcgcgt caggctgacg ctaatctttt taattctggc
    56821 ctcggtgacc tggctgcttt ccagctttgt cgcctggaaa caaacaacgg ataacgtcga
    56881 tgaattgttc gacacccaac tgatgctgtt tgccaaacgg ttaagtacgc tcgatctcaa
    56941 cgaaatcaac gcggcggatc gcatggcaca gacgccaaat aaattaaaac acggtcatgt
    57001 tgatgacgat gcgctgacct ttgccatctt tacccacgac ggcagaatgg tccttaatga
    57061 tggcgataac ggagaagata ttccctatag ctatcaacgg gaaggttttg ctgacgggca
    57121 actggtcggt gaagacgatc cttggcgttt tgtctggatg acctcacctg atggcaaata
    57181 tcgcatcgtt gttggccagg aatgggaata ccgtgaagac atggcgctgg cgattgttgc
    57241 cgggcaattg atcccgtggc tggtcgcact gccgattatg ttaatcatca tgatggtact
    57301 actgggtcgt gaactcgcgc cgctgaacaa actggcgctg gcactacgta tgcgtgaccc
    57361 tgactcggaa aaaccactaa acgcgactgg cgtacccagc gaagtgcggc cactggttga
    57421 gtcgctaaat caactgttcg cccgcacaca tgcgatgatg gttcgagaac gacgctttac
    57481 ctccgatgca gctcacgaac tacgtagccc gttaacagcg ctgaaagtgc aaaccgaagt
    57541 tgcgcagctc tctgacgatg atccgcaggc gcggaaaaaa gcactgctcc aattacattc
    57601 cgggatcgat cgcgctactc gtctggttga tcaactgctc acgctatcgc ggctggactc
    57661 actggataac cttcaggacg tcgcggagat cccgcttgaa gatctcctac aatcgtcggt
    57721 gatggatatt taccacacgg cgcagcaggc gaaaattgac gtgcgactga cactcaatgc
    57781 ccacggcatc aaacgcaccg gacaaccgct attgctaagt ttgttggtgc gaaatttgct
    57841 ggataacggc cccggtgtga caccagaggc actggcgcga attggcgaac gcttctatcg
    57901 cccacccgga caaaccgcta ccggcagtgg acttgggcta tcgattgtcc agcgaatcgc
    57961 caagctgcat gacatgaatg ttgagtttgg gaatgcggaa caaggtggat ttgaggcgaa
    58021 ggtaagctgg taacttctct gtctatgaga gccgttaaaa cgactctcat agattttact
    58081 aatagcaaaa tataaaccgt ccccaaaaaa gccaccaacc acaaaccaga caaataacga
    58141 gcgagaatac atatagccaa aactcgaaca tttcccgcat aaagagtttt cttaagataa
    58201 gaataataag tggcgtaaga agaaaaaatg ctgcaatcat tgcttgcaat atacgatctt
    58261 cgcctgaaac ggaaggtata ctgcaaatat tgcctcccga ctcaagcatc cagtcatact
    58321 tattgaaagc aaaaaaagaa tctattaacc aataagtaag cattatatat agcaatgctt
    58381 caaatattgt tttaattttc tgctttaaca tatatcagta cgctcataaa aatccattta
    58441 taattatcaa catgacacgt tgatattttc atcttttact tattcaaagc catattttca
    58501 atccccaaaa aaaccgaacc aaacgtgtaa attattccgg tgcgagtaag aattatgagg
    58561 aatggctatc agtattgtca ttttcagaaa atatttatcc tgcatcggtg agtcagagct
    58621 agatcagact tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt tcacagtacc
    58681 gctctgtgac gtactatagt cgacaaacgt ctcaccttga gattaaaaaa tgagcaacat
    58741 cctgattatc aacggcgcga aaaaattcgc ccactccaat ggtcaactga acgacaccct
    58801 gaccgaagtc gcggatggca cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc
    58861 cgacagcgac tacaatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat
    58921 ctggcagatg ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat atattgatga
    58981 tgtattcacc gaaggtcacg ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc
    59041 gtcgaaaaaa tacggttccg gcagcctggt acagggcaaa aaatatatgc tttctctaac
    59101 ctggaacgca ccaatggaag ccttcaccga aaaagatcag ttcttccacg gcgttggcgt
    59161 tgacggtgtg tatctgccgt tccataaagc aaaccaattc ctcggtatgg aaccgctgcc
    59221 gacatttatc gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata
    59281 tcgcaagcat cttgtggaaa tttttggtta actagagctc aggctttaga aggagttaac
    59341 catgcttacc gtaatcgcag aaatccgtac tcgtcctggt caacatcacc gtcaggcggt
    59401 attggatcag tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta
    59461 tgcgccaatg gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat
    59521 cgtgatgatt gagcagtggg aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca
    59581 catgaaggcg tacagcgaag ccgtaaaagg cgacgtgctg gagatgaata tccgtattct
    59641 gcagccaggg atttaatcct gccttgtttg cccggccatc ctgaccgggc aatgttcttt
    59701 cctttaaacc tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc
    59761 cgagcgtttc ttcgccagca gcatatccat catcgcgtca gtacgctgat cgtcttcatc
    59821 atcgatagtc aactgcacca gacggcgagt gttcggatca agcgtggttt cacgcaattg
    59881 catcgggttc atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg
    59941 ttttaattgc tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt
    60001 cccgagatca atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa
    60061 cgcgcggaag tgttttacga acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga
    60121 gtccgcatcc gcgaggatac agattttgcc ataacgaagc tggctcagat cgtcgctgtc
    60181 aggatcgata ccgatcgcta ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc
    60241 ggaagaaact tcccaggtgt taaggatctt acctttcagc ggcatgatcg cctgatattc
    60301 acgatcgcgc gcctgcttgg cagatccgcc tgcggagtca ccttccacta ggaacagttc
    60361 ggtacggtta aggccctgcg cggtacaatc agccagtttg ccaggcagcg ccgggccgct
    60421 ggtcagcttt ttacgcacca cttttttagc cgcacgcata cgacgctggg cgctggaaat
    60481 cgccatctct gccagcaact ccgccgcctg aacgttctgg ttcagccaca ggatgaaggc
    60541 atctttcacc acgcccgaaa cgaatgccgc acactgacgc gaagagagac gctctttggt
    60601 ctgcccggca aactgcggat cctgcatttt aaccgacaga acataggcgc agcgatccca
    60661 gatatcttcc gccgacagct tcacgccgcg cggcagaatg ttgcggtatt cacagaactc
    60721 acgcatcgcg tccaacaggc cctgacgcag accattaaca tgggtaccgc cctgcatcgt
    60781 tgggataagg ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag
    60841 cgcccagtcc acagcttcag tatcgccagc gaaattaccg ataaacggtt tttctggcag
    60901 cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca
    60961 gcgctgttcg gtgttgttga tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac
    61021 tgctttggcc ttcagcacat gcgtcaggcg tgaaacagaa aaacgcgggc tgtcaaagaa
    61081 ggtttcatcc ggccagaagt gcacgctggt accggtattg cgtttaccgc aagtgccgac
    61141 aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc
    61201 gcggcgcacg ttaacttcta cgcgcttcga caaggcgtta actaccgaaa tccccacgcc
    61261 atgcaggccg ccagagaact ggtaattttt gttagagaat ttaccgcctg catgcagacg
    61321 gcaaagaatc agttcaaccg ccggtactcc ttcttccggg tgaatatcca ccggcatccc
    61381 gcgcccatcg tcaatcactt ctaacgactg gtcagcatgt aagattacat ccacgcgttt
    61441 tgcgtgaccc gccagtgctt catcgacact gttatcaata acttcttgcc ccaaatggtt
    61501 agggcgagtg gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag
    61561 tacctcaatg gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg
    61621 atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc
    61681 ccgtgaatgc gtggttgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca
    61741 ccgcctggcg gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg
    61801 gcgcttccag cgggtcaatc tgcatgactt tgagatcgta aatatggcgt gactctagca
    61861 catattgctg cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcaattcaa
    61921 acgggcgtac cgccgggttt accaccactg cgggcagcat aaaacattgc gacaaccagg
    61981 tggcgtaata tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca
    62041 ggacaatgga ttccagcagt tctgccgcgt cggaaggata cggcggcaac tgcggaatga
    62101 tcatctcaac gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag
    62161 agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga
    62221 agcggtatca ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc
    62281 atcagcatgt aactcgagag tacgccagcc gggcgcgata gtatccagcg taaagttgga
    62341 acagtgcggc ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca
    62401 gtcgagatcc agctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt
    62461 cgccagcacg gtatccagtt cgcccgcgtt acgcagactg tgttgatcga gccaactaca
    62521 acccgcaggt agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc
    62581 cagtttacgt tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa
    62641 cacctggcta tccagcaaca ggatttgcca ttgctcacca ataaacaccc gcttcgccgg
    62701 ggagataccc gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg
    62761 cagccagacg cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc
    62821 agaggattga tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg
    62881 tggacgaatc gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaacagggc
    62941 ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt aaaatccttc ctctggcctc
    63001 accagccaga ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta
    63061 gtaaaccgga atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa
    63121 ctggttaatt tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg
    63181 cgctttgaag cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata
    63241 cagacgcacc gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat
    63301 tgtcactagg gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc
    63361 cacctgatag cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg
    63421 tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat
    63481 ttatgcccac tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac
    63541 cgacgctgcg ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac
    63601 cacatgaacg cgaatatctt cgttttcatc agccagacca tgaataccgc ttgcggtcgt
    63661 ggcgtccact tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag
    63721 gaaacttaac accggtttgg tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg
    63781 acgggcaaca tcttccacac tttcaccctc ttcaatcatc ccggcaacca tctccagtag
    63841 ccagggggtt tcgctggtgt cgtacgcggc aatccgaatc tgctcaatca gcacaacttc
    63901 atcacgcact gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg
    63961 ccgtacctca tgactcattt gcccgttgaa tagacgatga cgaaatctat aaagatctaa
    64021 tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa
    64081 tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc ctagcactaa tagtgaatta
    64141 aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt catatgttaa attgaggcac
    64201 attaacgccc tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg
    64261 caataatttt acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag
    64321 aaattgctcc ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc
    64381 gagaacctga tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct
    64441 gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca
    64501 cagctaggtt taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc
    64561 aactcgaacg cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa
    64621 tggcgtgcgt taacgctgca ggaaaaagca gcagggattc aggacgtcac atatcagacc
    64681 gatcagcaaa ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt
    64741 gacgttcttt cctatacaca ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc
    64801 acccaacgtt ttaacgtggg cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag
    64861 tacgataccg tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag
    64921 cagctgcgcc agatcaccgg taactactat ccggaactgg cggcgctgaa tgtcgaaaac
    64981 tttaaaaccg acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac
    65041 ctgtcgctgt tacaggcacg cttgagccag gacctggagc gcgagcaaat tcgccaggcg
    65101 caggatggtc acttaccgac tctggattta acggcttcta ccgggatttc tgacacctct
    65161 tatagcggtt cgaaaacccg tggtgccgct ggtacccagt atgacgatag caatatgggc
    65221 cagaacaaag ttggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg
    65281 caggtgaaac aggcacagta caactttgtt ggtgccagtg agcaactgga aagcgcacat
    65341 cgtagcgtcg tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcttc tatcagtagt
    65401 attaacgcct acaaacaagc cgtagtttcc gcgcaaagct cattagacgc gatggaagcg
    65461 ggctactcgg tcggtacgcg taccattgtt gatgtgttgg atgcgaccac cacgctgtac
    65521 aacgccaagc aagagctggc gaatgcgcgt tataactacc tgattaatca gctgaatatt
    65581 aagtcagccc tgggtacgtt gaacgagcag gatttgctgg cactgaacaa tgcgctgagc
    65641 aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt
    65701 gctgatggtt atgcgcctga tagcccggca cccgtcgttc agcaaacatc cgcacgcact
    65761 accaccagta acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc
    65821 gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc
    65881 ttcaatttcg accagccatc ctctattctg atgggtattt accactggtc ccggaagaca
    65941 aaaatgaaac ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcacgc
    66001 catctgacac cagtcgctct cgcggttgcc actgttttta tgctggctgg ctgtgaaaag
    66061 agtgatgaaa cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc
    66121 aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg
    66181 ccgaaatacg ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgtcagcaa
    66241 gcaccagccc aggctggcat ggcaccagaa aaccaggcgc aggctcagca atccagcggg
    66301 agtttctgga tgccgctgat ggccggttac atgatggggc gtctgatggg cggcggcgcg
    66361 ggatttgcac agcagccgct gttctcctcg aaaaacccgg ccagtccggc ttacggtaaa
    66421 tataccgacg cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta
    66481 ccgaagacgg caatggcacc aaaaccggcg accaccacta ccgttacccg tggcggtttt
    66541 ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg caaccggcac ctcttctcgt
    66601 tcaatgggtg gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg
    66661 agaaagccca cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg
    66721 aagatgctta ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg
    66781 aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga
    66841 ccaaattccg cataccaaaa cacacctgga gttttgtgcg tcaatcatgg ctgactcacc
    66901 agccatcgct ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa cctaaacttc
    66961 tggaaaataa cgccgatacg ccaacgtcac tatacgaggc ggcgttcttt cagtggatct
    67021 ggctggaaga tcagcttaac gccggtaact tgtcggaggg cagcgaccag tttaacagtc
    67081 tgcaagaaaa actgatcgat cgcttcgttg agctgcgtga acagtatggc ttccagttgc
    67141 tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg
    67201 actgcgcaac cgaagctgaa atcgccaccg agttcctcta catcgacgag ataggtttag
    67261 gcgaaaaagg ccagttcacg gatttacagg atcaggtgat ttctaacctg ttcaagcttt
    67321 atccgtggga atttatgctg cgcgaaatgt tctccaccaa gctggaagat gctggcgtac
    67381 gctggctgga accggcgtgg aagagcatta tctccaacaa ggcacttcta ccgctactgt
    67441 gggagatgtt cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc
    67501 cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga
    67561 tcattgagaa cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga
    67621 ttgttcagca attccacccg ttaccgaaat tcggcgacag ctatatgctg attggtagct
    67681 ggctggtgaa cgatcaacct gccggaattg gcattcgtga agaccgtgcg ttgatcaccc
    67741 aggatatgtc tcggttttat ccacatattt ttgttgaata agccacgata ccggatggca
    67801 ctcgccatcc ggtaattgtt agcctatctg caccgacagc atacttaaac tgcccatttc
    67861 tataccatcg accggaatgg taattggctc ctgcccatcc cacacaccta acacatacaa
    67921 caacggcaga tagtgctctg gcgttgggtt cgataacgcg ccaccttcat ggtcgagata
    67981 attcaccaga ggatgctgct ctaccggccc ttgccacgtc agattcgctt tcacatactc
    68041 attaaacgac gtcgcccacg gatacggtga actatcaccg tgccacttca ctgtgcgcag
    68101 gttatgcacc acgttaccgc tggcgaccaa cattattcct tcatcacgca gcgccgcgag
    68161 tttgcgcccc atttcgaaat gccaggcgac aggtttgcta ctgtcgatac tcaactgcac
    68221 catagggata tcggcgtcag gatacatctt aatcagcacg ccccacgagc cgtggtcaaa
    68281 gccccaggct tctttatcca gcgtcaccgg gatcggcgct aacagctcaa ccagacgctg
    68341 tgccagcgca ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc
    68401 accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa
    68461 ccagtgagcc gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg
    68521 ccagctgcgg gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa
    68581 caatgctggc atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat
    68641 atcctcacaa tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc
    68701 agttattttg acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc
    68761 tctcattctg accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct
    68821 cggacaaaaa ccctcgaacc gcttactggc gttttcgcta ggttttgcgg cggggatcat
    68881 gttgctcatc tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc
    68941 ggtgttgggt tatggaatgt ttatcttcgg tctgcttggc tactttggcc tggaccgcat
    69001 gttgccgcat gcgcatccgc aggatttaat gcaaaaatcg gtgcagctgt tgccaaaatc
    69061 gatcaagcgc acagccattc tgctcactct cggcatcagt ctgcataact tcccggaagg
    69121 gattgccacc tttgtcacgg cgagcagcaa cctggagctg ggatttggca tcgcactggc
    69181 cgtcgcgttg cacaatatcc ctgaaggtct ggcagtggca ggcccggttt atgcggcaac
    69241 ggggtctaaa cgcaccgcga ttctgtgggc ggggatttct ggactagcag aaattcttgg
    69301 tggtgtgctg gcgtggttaa tcctcggtag catgatttcc ccggtggtca tggcggcaat
    69361 catggcggcg gttgcaggaa ttatggtggc gctctcggtt gatgaattaa tgccgctcgc
    69421 caaagagatt gaccctaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat
    69481 gggattcagt ttagtgctgc tacaaacggc gggaattggt taaaaacaac atcagaagtt
    69541 gtccagcaca tggacaactt ctgccagctt cacgaaagcc ttattctacc ttttcggctg
    69601 tctcttctct cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct
    69661 ggcgaaacgc ccgacaataa cgtttcaaat gcaggaaata acccaccaca accagagcaa
    69721 tcaatacaat ccagtaccag gcgataaaca tccttttacc cttaatattt aacaatacta
    69781 aacatttagc gcataaattt cacatatcct ttttcggata tattcatatg gtcgcataat
    69841 aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt
    69901 ttctcataga aagagaagcg gaattaacat cttgcatagc agggcaaaat aaaaaccgaa
    69961 gccagctaca acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc
    70021 tttacgctca tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt
    70081 gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt
    70141 cagctcacac agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc
    70201 ttcagtatga ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg
    70261 gcccgggcga ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt
    70321 aatacgatca gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt
    70381 aaaaccggtg ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt
    70441 acggcgatct tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc
    70501 catctgctca acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc
    70561 ttcatcatca agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac
    70621 acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca
    70681 ctaaaattat ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata
    70741 acgtgagcgg gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc
    70801 ggccccggaa ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc
    70861 tttcaactga gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga
    70921 ttcactataa cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat
    70981 gacttaccct tatcgcacta caatggtact caacacctat cagtacaggg aaactacaat
    71041 gattgacccg aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg
    71101 aatcagggag ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct
    71161 gacgcgcctc gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg
    71221 gactcgtgag aaactggcgc tgcttgaaca aagaatcagc gaactggaaa atcgcagtac
    71281 tgagatcaaa aagcagccag atccagagac tctcccccca acgctgtaaa catttgtctt
    71341 taatgcactg taaattatga attaatcctc taatttacag tgttttctat tacattatcg
    71401 cttaatccca ttcagagtat catcacaaaa atgaatgatt gcaattgaaa atataattgt
    71461 tatgtattct ttaagtttta cttaatcgat atttagactg gccc
//
LOCUS       DAJVRL010000005        70705 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVRL010000005 DAJVRL010000000
VERSION     DAJVRL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.005"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..794
                     /locus_tag="OQN02_000315"
     CDS             <1..794
                     /locus_tag="OQN02_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1641245.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="OQN02_000316"
     CDS             805..1896
                     /locus_tag="OQN02_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1641246.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OQN02_000317"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OQN02_000317"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1641247.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="OQN02_000318"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="OQN02_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1641248.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="OQN02_000319"
     CDS             4858..5253
                     /locus_tag="OQN02_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1641249.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="OQN02_000320"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="OQN02_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1641250.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="OQN02_000321"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="OQN02_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1641251.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="OQN02_000322"
     CDS             complement(6562..7602)
                     /locus_tag="OQN02_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1641252.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="OQN02_000323"
     CDS             complement(7675..8310)
                     /locus_tag="OQN02_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1641253.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="OQN02_000324"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="OQN02_000324"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1641254.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="OQN02_000325"
     CDS             complement(8936..9379)
                     /locus_tag="OQN02_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1641255.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="OQN02_000326"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="OQN02_000326"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1641256.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="OQN02_000327"
     CDS             complement(9719..10222)
                     /locus_tag="OQN02_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1641257.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="OQN02_000328"
     CDS             complement(10216..10740)
                     /locus_tag="OQN02_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1641258.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="OQN02_000329"
     CDS             10949..11944
                     /locus_tag="OQN02_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1641259.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     HYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="OQN02_000330"
     CDS             11953..12831
                     /locus_tag="OQN02_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1641260.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="OQN02_000331"
     CDS             12912..13919
                     /locus_tag="OQN02_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS1641261.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OQN02_000332"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OQN02_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1641262.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OQN02_000333"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OQN02_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1641263.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OQN02_000334"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OQN02_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1641264.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OQN02_000335"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OQN02_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1641265.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OQN02_000336"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OQN02_000336"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1641266.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OQN02_000337"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OQN02_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1641267.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OQN02_000338"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OQN02_000338"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1641268.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OQN02_000339"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OQN02_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1641269.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OQN02_000340"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OQN02_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1641270.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OQN02_000341"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OQN02_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1641271.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OQN02_000342"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OQN02_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1641272.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="OQN02_000343"
     tRNA            complement(27653..27729)
                     /locus_tag="OQN02_000343"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="OQN02_000344"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="OQN02_000344"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1641273.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="OQN02_000345"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="OQN02_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="OQN02_000346"
     tRNA            complement(31513..31599)
                     /locus_tag="OQN02_000346"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OQN02_000347"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OQN02_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1641274.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OQN02_000348"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OQN02_000348"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1641275.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OQN02_000349"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OQN02_000349"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1641276.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OQN02_000350"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OQN02_000350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1641277.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OQN02_000351"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OQN02_000351"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1641278.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="OQN02_000352"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="OQN02_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1641279.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OQN02_000353"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OQN02_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1641280.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="OQN02_000354"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="OQN02_000354"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1641281.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OQN02_000355"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OQN02_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1641282.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="OQN02_000356"
     CDS             complement(41211..42176)
                     /locus_tag="OQN02_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1641283.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OQN02_000357"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OQN02_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1641284.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OQN02_000358"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OQN02_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1641285.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="OQN02_000359"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="OQN02_000359"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1641286.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="OQN02_000360"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="OQN02_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1641287.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OQN02_000361"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OQN02_000361"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1641288.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OQN02_000362"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OQN02_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1641289.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OQN02_000363"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OQN02_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1641290.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OQN02_000364"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OQN02_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1641291.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OQN02_000365"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OQN02_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1641292.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OQN02_000366"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OQN02_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1641293.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OQN02_000367"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OQN02_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1641294.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="OQN02_000368"
     CDS             49714..50679
                     /locus_tag="OQN02_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1641295.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="OQN02_000369"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="OQN02_000369"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1641296.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="OQN02_000370"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="OQN02_000370"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1641297.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="OQN02_000371"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="OQN02_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1641298.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="OQN02_000372"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="OQN02_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1641299.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="OQN02_000373"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="OQN02_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1641300.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="OQN02_000374"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="OQN02_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1641301.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="OQN02_000375"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="OQN02_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1641302.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="OQN02_000376"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="OQN02_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1641303.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="OQN02_000377"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="OQN02_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1641304.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="OQN02_000378"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="OQN02_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1641305.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58504
                     /gene="yrbL"
                     /locus_tag="OQN02_000379"
     CDS             57878..58504
                     /gene="yrbL"
                     /locus_tag="OQN02_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1641306.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="OQN02_000380"
                     /pseudo
     CDS             complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="OQN02_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="OQN02_000381"
     CDS             complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="OQN02_000381"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1641307.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="OQN02_000382"
     CDS             complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="OQN02_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1641308.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62540..63469)
                     /locus_tag="OQN02_000383"
     CDS             complement(62540..63469)
                     /locus_tag="OQN02_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1641309.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64144..68604
                     /gene="gltB"
                     /locus_tag="OQN02_000384"
     CDS             64144..68604
                     /gene="gltB"
                     /locus_tag="OQN02_000384"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1641310.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68617..70035
                     /gene="gltD"
                     /locus_tag="OQN02_000385"
     CDS             68617..70035
                     /gene="gltD"
                     /locus_tag="OQN02_000385"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1641311.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70219..70698
                     /locus_tag="OQN02_000386"
     CDS             70219..70698
                     /locus_tag="OQN02_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCS1641312.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17316 a        19177 c        17550 g        16662 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccgccattac cgtcatggtg cggtaaaaga agacgttacg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgca
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcagtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa cgccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctactgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgcattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accattatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt cgtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caagctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagttagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatca ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgataaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatctc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggtggcga taaagagatc cgccgtgagt taaagtacta
    58021 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    58081 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    58141 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    58201 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    58261 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    58321 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    58381 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    58441 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    58501 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    58561 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    58621 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    58681 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    58741 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    58801 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gattttggcc
    58861 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    58921 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    58981 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    59041 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    59101 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    59161 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    59221 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    59281 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    59341 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg
    59401 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    59461 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    59521 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    59581 tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    59641 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    59701 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    59761 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    59821 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    59881 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    59941 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    60001 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    60061 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    60121 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    60181 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    60241 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    60301 tcctcaacaa tgcctttttt atcctgcgcc gtcagattca actccagcac gctgacatag
    60361 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    60421 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    60481 gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    60541 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    60601 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    60661 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    60721 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    60781 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    60841 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    60901 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    60961 ttgccctgtt cactggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    61021 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    61081 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    61141 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    61201 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    61261 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    61321 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    61381 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    61441 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    61501 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    61561 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    61621 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    61681 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    61741 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    61801 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc
    61861 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    61921 ttatcttcgt tacgataaaa aaccaagtcg ggtgaagcat caaggaagga acgtaagaat
    61981 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    62041 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    62101 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    62161 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    62221 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    62281 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    62341 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    62401 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    62461 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    62521 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    62581 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    62641 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    62701 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    62761 caataccgtt caaacgcacc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    62821 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    62881 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    62941 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    63001 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    63061 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    63121 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    63181 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    63241 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    63301 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    63361 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    63421 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    63481 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctggg
    63541 tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    63601 gcacaatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    63661 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    63721 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    63781 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagct
    63841 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    63901 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    63961 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    64021 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    64081 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    64141 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    64201 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    64261 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    64321 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    64381 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    64441 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    64501 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    64561 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgccg tctgtttatc
    64621 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    64681 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    64741 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    64801 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    64861 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    64921 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    64981 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    65041 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    65101 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    65161 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    65221 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    65281 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    65341 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    65401 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    65461 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    65521 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    65581 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    65641 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    65701 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    65761 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    65821 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    65881 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    65941 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    66001 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    66061 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    66121 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    66181 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    66241 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    66301 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    66361 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    66421 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    66481 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    66541 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    66601 aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    66661 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    66721 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    66781 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    66841 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    66901 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    66961 gaaggtggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    67021 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    67081 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    67141 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    67201 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    67261 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    67321 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    67381 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    67441 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    67501 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    67561 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    67621 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    67681 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    67741 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    67801 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    67861 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    67921 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    67981 gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt
    68041 aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc
    68101 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    68161 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    68221 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    68281 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    68341 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    68401 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    68461 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    68521 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    68581 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    68641 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    68701 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    68761 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    68821 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    68881 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    68941 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    69001 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    69061 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    69121 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    69181 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcacaggc
    69241 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    69301 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    69361 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    69421 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    69481 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    69541 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    69601 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    69661 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    69721 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    69781 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    69841 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    69901 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    69961 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    70021 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    70081 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    70141 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    70201 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    70261 atccacaacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    70321 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    70381 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    70441 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    70501 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    70561 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    70621 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    70681 gtatattcat gcaaataaac gaagg
//
LOCUS       DAJVRL010000006        65689 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVRL010000006 DAJVRL010000000
VERSION     DAJVRL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.006"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OQN02_000387"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OQN02_000387"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1641313.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQN02_000388"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQN02_000388"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1641314.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQN02_000389"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQN02_000389"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1641315.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQN02_000390"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQN02_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1641316.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQN02_000391"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQN02_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1641317.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQN02_000392"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQN02_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1641318.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OQN02_000393"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OQN02_000393"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1641319.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10140
                     /gene="mrcB"
                     /locus_tag="OQN02_000394"
     CDS             7606..10140
                     /gene="mrcB"
                     /locus_tag="OQN02_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1641320.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGGQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10360..12603
                     /gene="fhuA"
                     /locus_tag="OQN02_000395"
     CDS             10360..12603
                     /gene="fhuA"
                     /locus_tag="OQN02_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1641321.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12654..13451
                     /gene="fhuC"
                     /locus_tag="OQN02_000396"
     CDS             12654..13451
                     /gene="fhuC"
                     /locus_tag="OQN02_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1641322.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13451..14341
                     /gene="fhuD"
                     /locus_tag="OQN02_000397"
     CDS             13451..14341
                     /gene="fhuD"
                     /locus_tag="OQN02_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1641323.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14338..16320
                     /gene="fhuB"
                     /locus_tag="OQN02_000398"
     CDS             14338..16320
                     /gene="fhuB"
                     /locus_tag="OQN02_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1641324.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="OQN02_000399"
     CDS             complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="OQN02_000399"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1641325.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17860..19281
                     /gene="clcA"
                     /locus_tag="OQN02_000400"
     CDS             17860..19281
                     /gene="clcA"
                     /locus_tag="OQN02_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1641326.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19305..19370
                     /locus_tag="OQN02_000401"
     CDS             19305..19370
                     /locus_tag="OQN02_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641327.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19363..19707
                     /gene="erpA"
                     /locus_tag="OQN02_000402"
     CDS             19363..19707
                     /gene="erpA"
                     /locus_tag="OQN02_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1641328.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19754..20377)
                     /locus_tag="OQN02_000403"
     CDS             complement(19754..20377)
                     /locus_tag="OQN02_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1641329.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="OQN02_000404"
     CDS             complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="OQN02_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS1641330.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="OQN02_000405"
     CDS             complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="OQN02_000405"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1641331.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21990..23507
                     /gene="dgt"
                     /locus_tag="OQN02_000406"
     CDS             21990..23507
                     /gene="dgt"
                     /locus_tag="OQN02_000406"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1641332.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23637..25061
                     /gene="degP"
                     /locus_tag="OQN02_000407"
     CDS             23637..25061
                     /gene="degP"
                     /locus_tag="OQN02_000407"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1641333.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25216..26372
                     /gene="cdaR"
                     /locus_tag="OQN02_000408"
                     /pseudo
     CDS             25216..26372
                     /gene="cdaR"
                     /locus_tag="OQN02_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26461..26847)
                     /locus_tag="OQN02_000409"
     CDS             complement(26461..26847)
                     /locus_tag="OQN02_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1641334.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="OQN02_000410"
     CDS             complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="OQN02_000410"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1641335.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="OQN02_000411"
     CDS             complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="OQN02_000411"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1641336.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30750..31544)
                     /gene="map"
                     /locus_tag="OQN02_000412"
     CDS             complement(30750..31544)
                     /gene="map"
                     /locus_tag="OQN02_000412"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1641337.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31912..32637
                     /gene="rpsB"
                     /locus_tag="OQN02_000413"
     CDS             31912..32637
                     /gene="rpsB"
                     /locus_tag="OQN02_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1641338.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32895..33746
                     /gene="tsf"
                     /locus_tag="OQN02_000414"
     CDS             32895..33746
                     /gene="tsf"
                     /locus_tag="OQN02_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1641339.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33893..34618
                     /gene="pyrH"
                     /locus_tag="OQN02_000415"
     CDS             33893..34618
                     /gene="pyrH"
                     /locus_tag="OQN02_000415"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1641340.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34910..35467
                     /gene="frr"
                     /locus_tag="OQN02_000416"
     CDS             34910..35467
                     /gene="frr"
                     /locus_tag="OQN02_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1641341.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35559..36755
                     /gene="ispC"
                     /locus_tag="OQN02_000417"
     CDS             35559..36755
                     /gene="ispC"
                     /locus_tag="OQN02_000417"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1641342.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36944..37702
                     /gene="ispU"
                     /locus_tag="OQN02_000418"
                     /gene_synonym="uppS"
     CDS             36944..37702
                     /gene="ispU"
                     /locus_tag="OQN02_000418"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1641343.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37715..38572
                     /gene="cdsA"
                     /locus_tag="OQN02_000419"
     CDS             37715..38572
                     /gene="cdsA"
                     /locus_tag="OQN02_000419"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1641344.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38584..39936
                     /gene="rseP"
                     /locus_tag="OQN02_000420"
     CDS             38584..39936
                     /gene="rseP"
                     /locus_tag="OQN02_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1641345.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39966..42398
                     /gene="bamA"
                     /locus_tag="OQN02_000421"
     CDS             39966..42398
                     /gene="bamA"
                     /locus_tag="OQN02_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1641346.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42520..43005
                     /gene="skp"
                     /locus_tag="OQN02_000422"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42520..43005
                     /gene="skp"
                     /locus_tag="OQN02_000422"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1641347.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43009..44034
                     /gene="lpxD"
                     /locus_tag="OQN02_000423"
     CDS             43009..44034
                     /gene="lpxD"
                     /locus_tag="OQN02_000423"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1641348.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44139..44594
                     /gene="fabZ"
                     /locus_tag="OQN02_000424"
     CDS             44139..44594
                     /gene="fabZ"
                     /locus_tag="OQN02_000424"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1641349.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44598..45386
                     /gene="lpxA"
                     /locus_tag="OQN02_000425"
     CDS             44598..45386
                     /gene="lpxA"
                     /locus_tag="OQN02_000425"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1641350.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45386..46534
                     /gene="lpxB"
                     /locus_tag="OQN02_000426"
     CDS             45386..46534
                     /gene="lpxB"
                     /locus_tag="OQN02_000426"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1641351.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46531..47127
                     /gene="rnhB"
                     /locus_tag="OQN02_000427"
     CDS             46531..47127
                     /gene="rnhB"
                     /locus_tag="OQN02_000427"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1641352.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47164..50646
                     /gene="dnaE"
                     /locus_tag="OQN02_000428"
     CDS             47164..50646
                     /gene="dnaE"
                     /locus_tag="OQN02_000428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1641353.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50659..51618
                     /gene="accA"
                     /locus_tag="OQN02_000429"
     CDS             50659..51618
                     /gene="accA"
                     /locus_tag="OQN02_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1641354.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51717..53858
                     /gene="ldcC"
                     /locus_tag="OQN02_000430"
     CDS             51717..53858
                     /gene="ldcC"
                     /locus_tag="OQN02_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1641355.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53915..54304
                     /locus_tag="OQN02_000431"
     CDS             53915..54304
                     /locus_tag="OQN02_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1641356.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54369..55667
                     /gene="tilS"
                     /locus_tag="OQN02_000432"
     CDS             54369..55667
                     /gene="tilS"
                     /locus_tag="OQN02_000432"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1641357.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55716..55976)
                     /gene="rof"
                     /locus_tag="OQN02_000433"
     CDS             complement(55716..55976)
                     /gene="rof"
                     /locus_tag="OQN02_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1641358.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55963..56163)
                     /locus_tag="OQN02_000434"
     CDS             complement(55963..56163)
                     /locus_tag="OQN02_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1641359.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56329..56874
                     /locus_tag="OQN02_000435"
     CDS             56329..56874
                     /locus_tag="OQN02_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1641360.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56871..57293
                     /gene="arfB"
                     /locus_tag="OQN02_000436"
     CDS             56871..57293
                     /gene="arfB"
                     /locus_tag="OQN02_000436"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1641361.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57307..58017
                     /gene="nlpE"
                     /locus_tag="OQN02_000437"
                     /gene_synonym="cutF"
     CDS             57307..58017
                     /gene="nlpE"
                     /locus_tag="OQN02_000437"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1641362.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58172..58996)
                     /locus_tag="OQN02_000438"
     CDS             complement(58172..58996)
                     /locus_tag="OQN02_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS1641363.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59049..60767)
                     /gene="proS"
                     /locus_tag="OQN02_000439"
     CDS             complement(59049..60767)
                     /gene="proS"
                     /locus_tag="OQN02_000439"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1641364.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="OQN02_000440"
     CDS             complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="OQN02_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1641365.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="OQN02_000441"
     CDS             complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="OQN02_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1641366.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="OQN02_000442"
     CDS             complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="OQN02_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1641367.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62959..63612)
                     /gene="metI"
                     /locus_tag="OQN02_000443"
     CDS             complement(62959..63612)
                     /gene="metI"
                     /locus_tag="OQN02_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1641368.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63605..64636)
                     /gene="metN"
                     /locus_tag="OQN02_000444"
     CDS             complement(63605..64636)
                     /gene="metN"
                     /locus_tag="OQN02_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1641369.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64824..65399
                     /gene="gmhB"
                     /locus_tag="OQN02_000445"
     CDS             64824..65399
                     /gene="gmhB"
                     /locus_tag="OQN02_000445"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1641370.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15530 a        16279 c        18103 g        15777 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccacca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatca ggcactttcc agccaaaaag
     2341 ctggtacagc gggatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaaaataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcaggt gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggctgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgtgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acagcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgagcg caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcccgcggca
     7921 gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa cgaaatggtg
     7981 aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg aatcagtctc
     8341 tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ttcttactgg
     8461 cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tggggggtca gccgcgtggt
     8821 ggggtgatct ctcctcagcc agcctttatg cagctggtgc gtcaggagtt gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca gttgcatccg
     9421 gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggta aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg ggtaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa
    10081 gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg tagtaattaa
    10141 catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccatctaa
    10201 gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc
    10261 gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa taatcattct
    10321 cgtttacgtt attattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct
    10381 cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg
    10441 tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca
    10501 cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg gcagtcagct
    10561 accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc
    10621 gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg
    10681 ggtgtctctg ttggtacgcg tggcgcatcc aacacctatg accacctgat cattcgcggt
    10741 tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt gcagggcaac
    10801 ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat tatgcgtggc
    10861 ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat ggtcagcaag
    10921 cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc
    10981 cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc ttatcgcctg
    11041 accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct
    11101 attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac
    11161 ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag
    11221 ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa gaacaacacc
    11281 tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa cgacaccttt
    11341 actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt
    11401 tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca
    11461 gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac
    11521 ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg
    11581 ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac
    11641 gactctgtgc cactgctcaa tctgtacaat ccggtgaatc ccgatttcga cttcaatgcc
    11701 aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca aacgggcgtt
    11761 tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg tcgttatgac
    11821 tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg tgatgacaaa
    11881 cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac accttacttc
    11941 agctatagcg aatcgtttga accttcttcg caagttggga aggatggtaa tattttcgca
    12001 ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt
    12061 gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag
    12121 ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg
    12181 aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac cgatgcggaa
    12241 tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct
    12301 tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac gctgggcacc
    12361 ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt
    12421 tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc
    12481 aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac
    12541 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac ctttcgtttc
    12601 taatttctct tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg
    12661 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
    12721 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
    12781 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
    12841 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
    12901 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
    12961 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
    13021 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
    13081 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
    13141 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc agcgctggat atcgcccacc
    13201 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
    13261 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
    13321 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
    13381 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
    13441 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
    13501 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
    13561 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
    13621 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
    13681 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
    13741 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
    13801 gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg cgcgtaaatc
    13861 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
    13921 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
    13981 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
    14041 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
    14101 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
    14161 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
    14221 catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt tttatggtgc
    14281 aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag gtaaagcgtg
    14341 agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
    14401 gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc gcaggccgcc
    14461 tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt gttgccgcgt
    14521 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
    14581 gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg cgcgcaactg
    14641 gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca gtttgctgcg
    14701 ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg gaaacggctg
    14761 tcgccggtaa cgctgatttt cgcggggttg gtagtgagcc tttattgcgg cgcaatcaat
    14821 cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg gagcactgga
    14881 acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt
    14941 gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga tgatggcgtg
    15001 gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc gctggcgatt
    15061 gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg gttgttcgcg
    15121 ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat gctggcgtcg
    15181 ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
    15241 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
    15301 ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga tcgtgtcgcg
    15361 actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt gttgatggct
    15421 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
    15481 ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
    15541 gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc
    15601 ccggaagtgc tggggattag ctccggcgcg gcgtttggtg tggtgttaat gctgtttctg
    15661 gtaccgggta atgcctttgg ctggttgtta cccgcaggca gtctcggggc ggcggtgacg
    15721 ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg catgttactg
    15781 gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca ggcaagtggt
    15841 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat
    15901 gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac tccgctgtgc
    15961 cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt agggatggcg
    16021 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
    16081 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
    16141 ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg
    16201 gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
    16261 ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca gagccgttaa
    16321 tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac acccgacgtg
    16381 cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcccgctt
    16441 caaacgctga cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa
    16501 aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa
    16561 agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc gcttcttctg
    16621 ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaatgc
    16681 ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc gggttaccgg
    16741 aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt acatcacgac
    16801 gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg caggttaaat
    16861 ctggcactac ggcgaaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca
    16921 cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga
    16981 actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg
    17041 caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac
    17101 aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg
    17161 tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt
    17221 caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatcg
    17281 tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca
    17341 ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg
    17401 cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc
    17461 aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg
    17521 ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca
    17581 caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg
    17641 gttcctgatt cgtagaaaaa gtgaatggct gctattccat gttattcata acaagttaaa
    17701 tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag
    17761 ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat
    17821 gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct
    17881 ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag
    17941 cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg
    18001 ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg
    18061 ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct ctgttcggcg
    18121 gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc agggggatcg
    18181 gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta
    18241 ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggg gctagggcgc
    18301 gaaggaccaa ccgtgcagat cggcggcaat atcagtcgca tggtgcttga tattttccgc
    18361 ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct
    18421 gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag
    18481 tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt
    18541 atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg
    18601 ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct
    18661 atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat
    18721 attaccaaat gggtactgat ggggggtgcg attggcggtc tgtgtggatt gctggggttt
    18781 gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat
    18841 ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac cttactctgc
    18901 ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg
    18961 ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct ggaggcgggg
    19021 acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc gccgttaacg
    19081 gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt
    19141 accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct atactcggcg
    19201 attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca
    19261 tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg attattattg
    19321 ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca
    19381 ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa
    19441 gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag
    19501 tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc
    19561 gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactac
    19621 accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc
    19681 ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa actatccggc
    19741 acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc
    19801 aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt
    19861 ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg
    19921 cagttgcgta gatttctgta cgtaaaatca tggggatttc gcggaccagt acatcacgaa
    19981 tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt
    20041 ccgcattaaa ggctttattc acgccaatgc cgacaaacac cgccagacca acggcgtcca
    20101 gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca
    20161 tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt
    20221 gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg cccagtacca
    20281 gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccgtag atggcaaata
    20341 ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct
    20401 gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg tgctgcgagg
    20461 ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg
    20521 agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca
    20581 atagcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg
    20641 ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg aatcgactct
    20701 tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta
    20761 tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc ggcttgttcg
    20821 gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc aatttgttca
    20881 atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc
    20941 cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt
    21001 tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag
    21061 tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg
    21121 gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc gagaaaagac
    21181 agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca ccagtgactc
    21241 aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga
    21301 ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt tgaaattgtg
    21361 gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg
    21421 gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc
    21481 acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc
    21541 gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac caaatgccgt
    21601 gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt
    21661 tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa
    21721 cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac
    21781 ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga tagtttgacg
    21841 gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat
    21901 tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg
    21961 ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa
    22021 actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga
    22081 tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa
    22141 agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga
    22201 tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc
    22261 ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa agcattgttg
    22321 agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcag
    22381 cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc cagcctctga
    22441 ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa ccgcttaacg
    22501 agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc
    22561 gtctggtgca tacattgatg cggatgaatc tcacttgggc acaggttggc ggtattttaa
    22621 aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga
    22681 aaaagccggg ttattatctt tctgaagaag cctatattgc ccgcttgcgt aaagaactta
    22741 atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct
    22801 cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc
    22861 tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg
    22921 tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc
    22981 agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac
    23041 gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag
    23101 atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt
    23161 ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat
    23221 tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa ctggtagaaa
    23281 aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc tcgacgcgcc
    23341 atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc
    23401 tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc
    23461 tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca ggcttttgta
    23521 aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt cgggctataa
    23581 aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga
    23641 aaaaaaccac attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc
    23701 tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg
    23761 caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa
    23821 ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt
    23881 tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc cagggcggta
    23941 atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg
    24001 ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg attaaagtcc
    24061 agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata
    24121 tcgcgctgat ccaaatccag aacccgaaaa aactgaccgc aattaagatg gcggattctg
    24181 atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga
    24241 cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct gaaaactacg
    24301 aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta
    24361 acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac ggcggcaaca
    24421 tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg
    24481 aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aattccgaac
    24541 tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta
    24601 attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta
    24661 agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg gtgggcagca
    24721 aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg gaactgcagc
    24781 agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga
    24841 tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg ggcactccgg
    24901 ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag caggcagtga
    24961 aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca
    25021 ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca accccttcct
    25081 gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc atacatcttc
    25141 atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg
    25201 cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg
    25261 gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg cgggcgaatt
    25321 atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt
    25381 tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca cggtgtgcgg
    25441 caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca
    25501 ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg
    25561 ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaagaactg
    25621 gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg gcgcaacggc
    25681 tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc
    25741 ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg
    25801 agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt
    25861 tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta
    25921 cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg
    25981 gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac
    26041 agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg
    26101 acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag
    26161 cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc
    26221 aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tatcggctta
    26281 atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt
    26341 atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga ggcagcgctg
    26401 gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt caattcggga
    26461 ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac
    26521 tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc
    26581 acgctgacag atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg
    26641 gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata
    26701 tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag
    26761 aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat
    26821 acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc
    26881 ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct
    26941 tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc
    27001 catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag
    27061 atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgagccag
    27121 aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca
    27181 gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac
    27241 agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc acagaccccg
    27301 ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac
    27361 caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt
    27421 cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct
    27481 ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac
    27541 aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcgccgatgt
    27601 taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga cgtaccgccg
    27661 ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt
    27721 tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa
    27781 tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa
    27841 ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt
    27901 cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt
    27961 caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc
    28021 ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt
    28081 tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa
    28141 tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc
    28201 gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat
    28261 gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc
    28321 gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa
    28381 taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat
    28441 ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc
    28501 ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa
    28561 tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat
    28621 gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt
    28681 tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca
    28741 gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt
    28801 gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct
    28861 tccagctatt ccacagtgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca
    28921 gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt
    28981 cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accagccagg
    29041 caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct
    29101 gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg
    29161 cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac aacggatggc
    29221 gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt
    29281 catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt
    29341 gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagtccg cgccgcaccg
    29401 ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata ttacacagcg
    29461 gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag taaatgccgg
    29521 tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca
    29581 taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga
    29641 gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca
    29701 gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct
    29761 cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat
    29821 cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc
    29881 acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc
    29941 cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt
    30001 gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc
    30061 catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg
    30121 aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc
    30181 caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca
    30241 acatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca
    30301 gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa
    30361 tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcaa
    30421 ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct
    30481 ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca ttgtcaaagg
    30541 catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt
    30601 catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtggag agagcggtgt
    30661 ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat
    30721 taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg
    30781 gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac aatagtatgc
    30841 tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg
    30901 cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat ccccggtttc
    30961 agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg
    31021 cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat
    31081 ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc
    31141 aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt cggcttaccg
    31201 acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg
    31261 ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact
    31321 tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc
    31381 gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg
    31441 cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg
    31501 cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag
    31561 cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat
    31621 tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc
    31681 gacgtctgat gctgtacaca gcgccaacaa ttattggtgt ccacgacgta tttgtggtat
    31741 aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc
    31801 actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta
    31861 atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact
    31921 gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg
    31981 aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt
    32041 gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat tgtttctcgc
    32101 aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct
    32161 gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac
    32221 tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac
    32281 ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa
    32341 ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta
    32401 atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg tattccggta
    32461 tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac
    32521 gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa
    32581 ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa
    32641 ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc
    32701 cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca tctgcggcgc
    32761 gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg
    32821 catatggccc cctttttcac ttttatatct gtgtggttta atgccgggca gatcacatct
    32881 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    32941 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    33001 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    33061 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    33121 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    33181 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    33241 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg
    33301 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    33361 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    33421 gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc
    33481 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    33541 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    33601 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    33661 tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    33721 ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg gcggcttctt
    33781 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    33841 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    33901 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    33961 cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga
    34021 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    34081 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    34141 gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    34201 tctgatgtcc gctattccat tgaatggcgt gtgcgatagc tacagctggg cagaagctat
    34261 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    34321 taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    34381 agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagattcaa ccgcaaccat
    34441 gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc
    34501 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata tgaacaaacc
    34561 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    34621 cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt
    34681 tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta
    34741 caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata aaatagccta
    34801 ataatccaga cgattacccg taatatgttt aatcagggct atacttagca cacttccact
    34861 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    34921 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    34981 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    35041 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    35101 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    35161 ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    35221 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    35281 gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    35341 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    35401 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    35461 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    35521 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    35581 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    35641 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg
    35701 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    35761 tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    35821 tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct gctgggctgt
    35881 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    35941 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    36001 cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc cagcataatc
    36061 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    36121 gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcttgcc
    36181 gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca
    36241 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag
    36301 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    36361 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    36421 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    36481 ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    36541 gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    36601 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    36661 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgagtgaag
    36721 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    36781 gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg
    36841 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    36901 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    36961 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    37021 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    37081 aaaatccgtc cgccgggctg tctcttttgc ggccaacaac ggtattgagg cgttaacgct
    37141 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    37201 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    37261 gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta ttcgtaaatc
    37321 tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga actacggtgg
    37381 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    37441 gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc atgaactggc
    37501 ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact ttttgctttg
    37561 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    37621 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    37681 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    37741 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    37801 cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt
    37861 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    37921 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    37981 ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    38041 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt
    38101 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    38161 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata
    38221 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    38281 ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg gttatggcat
    38341 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    38401 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    38461 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    38521 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    38581 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    38641 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    38701 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    38761 gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    38821 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg
    38881 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    38941 tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata
    39001 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    39061 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    39121 attacagtag cgccatttgg cagcgaccaa cggcgggatg taaagctcga tttacgtcac
    39181 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    39241 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    39301 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    39361 tttgtgatgc ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    39421 gggagtccct tgtctttgac attaatcccg gggagtaaac cgggtaatgg taaagcgatt
    39481 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    39541 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    39601 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    39661 gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    39721 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    39781 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    39841 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    39901 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    39961 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    40021 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    40081 ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat gatgaagata
    40141 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    40201 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    40261 tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg
    40321 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    40381 acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc
    40441 cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc
    40501 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    40561 tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag aaacagaaac
    40621 tggcgggcga tcttgaaacc ctgcgcagct actatctgga ccgcggttat gcccgtttca
    40681 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    40741 acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    40801 ggcactccgc tgaaattgag cagctgacta agatcgagcc gggcgagctg tataacggca
    40861 ctaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat ggttatgcct
    40921 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    40981 tgaacgttga tgtgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    41041 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggttgggga
    41101 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    41161 ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga
    41221 aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga
    41281 gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca
    41341 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    41401 ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    41461 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    41521 ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    41581 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct
    41641 ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    41701 acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag
    41761 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    41821 tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg
    41881 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    41941 ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg
    42001 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    42061 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    42121 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tcattcttct
    42181 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    42241 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    42301 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    42361 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    42421 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    42481 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42541 gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    42601 aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42661 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42721 atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg
    42781 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    42841 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42901 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    42961 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    43021 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    43081 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    43141 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    43201 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43261 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43321 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt
    43381 gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43441 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43501 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43561 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43621 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt
    43681 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43741 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43801 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43861 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43921 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43981 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    44041 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    44101 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    44161 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44221 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44281 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44341 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44401 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44461 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44521 aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44581 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44641 aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    44701 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44761 aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt aaccaggatt
    44821 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44881 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44941 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    45001 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    45061 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct
    45121 ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac gcaacgccgt
    45181 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45241 tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45301 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45361 gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga ttgccctggt
    45421 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45481 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45541 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45601 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga
    45661 tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta acctcaaaaa
    45721 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    45781 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    45841 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45901 gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt acgatgccca
    45961 ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt
    46021 cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccgct
    46081 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    46141 gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact
    46201 actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46261 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46321 ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg
    46381 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    46441 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46501 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag
    46561 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46621 gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46681 gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg
    46741 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    46801 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46861 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag ccgcgtacca
    46921 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46981 gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat
    47041 ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc
    47101 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    47161 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47221 ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47281 gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc
    47341 gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat
    47401 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctggcgttg
    47461 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47521 tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc
    47581 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47641 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47701 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact
    47761 aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47821 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47881 atgcgtagcg aagaggagat gtgtgagctg tttgccgaca tcccagaagc ccttgccaac
    47941 accgttgaga tcgccaaacg ctgtaacgta actatgcgtc ttggtgaata cttcctgccg
    48001 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagaaggc
    48061 ctggaagagc gtctggcatt tttattccct gacgaggaag aacgtcttaa acgccgcccg
    48121 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc
    48181 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg
    48241 ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat caccgacctc
    48301 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48361 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtggcg
    48421 gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa
    48481 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgaccgtatc
    48541 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48601 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc
    48661 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48721 accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc
    48781 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48841 cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48901 ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48961 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    49021 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc
    49081 cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca tggtcgtgaa
    49141 gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt actggagcca
    49201 acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt
    49261 tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa gccggaagag
    49321 atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49381 ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg
    49441 cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatccg
    49501 gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc
    49561 ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt
    49621 ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg cgccatcaaa
    49681 ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc
    49741 cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg
    49801 gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg
    49861 aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt
    49921 caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca atcctacgcc
    49981 agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg
    50041 tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc
    50101 gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc
    50161 gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg
    50221 gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag
    50281 caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc
    50341 agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa
    50401 tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac
    50461 cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat
    50521 cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc
    50581 gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt
    50641 gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag
    50701 ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt
    50761 aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc
    50821 ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccgca gcgtccttat
    50881 accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgtgcg
    50941 tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg
    51001 atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg
    51061 ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag
    51121 atgcctatca tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag
    51181 cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct tggcgtaccg
    51241 gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc
    51301 gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg
    51361 tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc
    51421 gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc
    51481 gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg
    51541 gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg
    51601 agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt
    51661 ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga
    51721 acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac
    51781 tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt
    51841 tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt
    51901 acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg ctttatgcct
    51961 tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt
    52021 tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacacca
    52081 acgaatatct tgataacatt acgccgccgt tcacgaaagc cttgtttacc tacgtcaaag
    52141 agcggaagta caccttttgt acgccggggc atatgggcgg caccgcatat caaaaaagcc
    52201 cggttggctg tctgttttat gattttttcg gcgggaatac ccttaaggct gatgtctcta
    52261 tttcggtcac cgagcttggt tcgttgcttg accacaccgg gccacatctg gaagcggaag
    52321 agtacatcgc gcggactttt ggcgcggaac agagttatat cgttaccaac ggaacatcga
    52381 cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgatcgacc
    52441 gcaactgcca taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc
    52501 tgaaaccgac gcgtaatgcg ttggggattc tgggggggat cccgcgccgt gaatttactc
    52561 gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc acaatggccg gttcatgcgg
    52621 tgatcaccaa ctccacctat gatggtttgc tctacaacac cgactggatc aaacagacgc
    52681 tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtatactcat tttcatccaa
    52741 tctaccaggg taaaagtggt atgagcggcg agcgtgttgc gggaaaagtt ttcttcgaaa
    52801 cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc ttcactgatc cacattaaag
    52861 gcgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca
    52921 gttatcccat tgttgcttcg gttgagacgg cggcggcgat gctgcgtggt aatccgggca
    52981 aacggctgat taaccgttcg gtggagcggg cgctgcattt tcgcaaagag gtccagcggc
    53041 tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag
    53101 ccgaatgcag gcccgttgcg cctggcgaac aatggcacgg ctttaacgat gcggatgccg
    53161 atcatatgtt tctcgatccg gttaaagtca ctattttgac accggggatg gacgaacagg
    53221 gcaatatgag tgaggagggg atcccggcgg cgctggtagc aaaattcctc gacgaacgtg
    53281 ggatcgtagt agagaaaacc ggcccttata acctgctgtt tctctttagt attggcatcg
    53341 ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaagcgc tcttacgatc
    53401 tcaacctgcg gatcaaaaat atgctgcccg atctctatgc tgaagatccc gatttctacc
    53461 acaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc
    53521 tttccggttt gatgttgcgg gcattcgata ctttgccgga aatgatcatg acgccacatc
    53581 aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg
    53641 gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgccag
    53701 gagaaatgct gaccaaagag agccgcacgg tactcgattt tctactgatg ctttgttccg
    53761 tagggcaaca ttaccccggt tttgaaacgg atattcacgg cgcgaaacag gacgaagacg
    53821 gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc
    53881 gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca
    53941 tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg atattcttgg
    54001 tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc
    54061 gcttaatggg caatatgtga ttgagctttt ctcttttccg ttcccgccgg agcgccccag
    54121 ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc
    54181 agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac
    54241 gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca
    54301 gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc
    54361 gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag attctggtgg
    54421 cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg
    54481 aaaatccggg cgtcactctg cgcgctattc atgtgcatca cggtttaagt gccaatgccg
    54541 atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg
    54601 aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg caggcacgtt
    54661 atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg caacatctcg
    54721 acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt
    54781 cggctatggc ggaagtttcg gagtttgctg gaacacggct tatccgcccg ttgcttgccc
    54841 gcacgcgggg ggaactggag cagtgggcat tggcgcatgg tttacgctgg attgaagacg
    54901 aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta gtgccgttat
    54961 tgcagcagcg ttggccgcat tttgcggaag caacggcccg cagcgccaca ctttgtgctg
    55021 aacaagagag tctgttggat gaactgctgg cggatgattt agcacactgc caaacgtcgc
    55081 aggggacgct gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc gcggcgatta
    55141 ttcgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ctggtgcgca
    55201 tctggcagga agtggcgctg gcgcgggaag atgcttcccc ctgtttacgt ttgggcgcct
    55261 ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa atccgtcacc gggcaaagcg
    55321 aaaccattgt gctgtggcag acgtggctac aaccgctgga attaccggcg gggctgggaa
    55381 ccgtacagct tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg
    55441 tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg caacggcgga cgtaagctga
    55501 agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt
    55561 tttatggaga aacgctgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg
    55621 aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct tagttaagtg aaagccggat
    55681 aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg attcgcttac caccaccgta
    55741 ccgatttcag ggtggctaaa gctggtaatt ttatccagac gcagctcgcg ggtttcgcca
    55801 gcggcctcga cgaccagata ctccacattt ttgcgggaga ctaaatcact ggcttttgcc
    55861 tgcaattttt cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc
    55921 tcaagattat cgtaatcatc acaattaatt ggttgatacg tatcattcat tgacataatc
    55981 gctcaccagt aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc
    56041 gtcgtcggca gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta
    56101 tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc
    56161 cacatgccct cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga
    56221 taaactctgt ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac
    56281 tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa
    56341 gcgacaattt ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat
    56401 gcctctctga cgctggcgcg ccacccttca gaaacccagg agcgtatgat gctgcgcttg
    56461 ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat
    56521 gatgagccgg aagcgtggct gcgtaacgat cacctgggca ttgatttgtg gattgagctg
    56581 gggctgccgg atgagcgacg aattaagaaa gcctgcaccc aggccgcaga agtggcgctg
    56641 tttacctata atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag caaatgtgtg
    56701 cagtttgcca atctttccgt ctggtatctg gacgatgaac aactggcgaa agtaagcgcc
    56761 tttgccgatc gtaccatgac gctgcaggca acgattcagg atggtgttat ctggttatcg
    56821 gatgataaga ataatctgga agttaactta accgcctggc aacaaccttc atgattgtga
    56881 tttcccgaca tgttgctatt cccgatggtg agcttgagat caccgccatt cgtgcgcagg
    56941 gcgctggcgg gcagcatgtt aataagacct caacggctat tcatctgcgt tttgacattc
    57001 gggcgtccag cctgccagag tattacaaag agcgtctgct cgccgccagc catcatttga
    57061 tcagcagtga tggcgtgatt gtcattaagg cacaggaata ccgcagtcag gaactgaacc
    57121 gcgaagccgc tctggcccgg ctggtggcag tgattaaaga tttaacaaaa gaacaaaaag
    57181 cccgacgacc cacgcggccc acccgtgcat cgaaagagcg caggctggca tcgaaagcac
    57241 aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag cggtcgggaa taaaaagaag
    57301 gaatggatgg tgaaaaaagc gatagtgaca gcgatggctg taatcagcct ctttactctg
    57361 atgggatgta ataatcgggc cgaagtcgat acgctttctc cggcgcaggc tgccgaactg
    57421 aaaccgatgc cgcaaagttg gcgcggcgtg ctaccgtgtg ccgattgcga aggaatcgaa
    57481 atctctctgt tcctcgaaaa agacggaaca tgggtgatga atgagcgtta tctcggtgct
    57541 cgtgaagaac cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc tgacaagctg
    57601 gtattaaccg acagcaaagg tgaaaagtca tattatcggg cgaaaggcga tgcgctggag
    57661 atgctcgatc gtgaaggcaa tccaattgaa tcgcagttca actatacgct ggaaccggca
    57721 caatccagtt tacctatgac gccgatgacc ctgcgaggca tgtattttta tatggctgat
    57781 gcggcgacct tcactgattg cgcgaccgga aaacgtttca tggtagcgaa taacgcagag
    57841 ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa aaccggtgtt actgtcagta
    57901 gaaggtcact ttacgcttga ggctaatccg gatactggtg cgccaaccaa agtattagcg
    57961 cccgatacgg cgggtaaatt ttacccgaat aaggattgca gtagtttggg gcagtaaacc
    58021 tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct gcgcttatca ggcctacagc
    58081 atataatgca acattttgaa ttagcacgat tttgtaggcc ggataaggcg tttacgccgc
    58141 atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg
    58201 ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
    58261 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
    58321 gcgccagtgt aagtggatgc ccggatttgg caaatgcatc agtgacaaat tccgaacaaa
    58381 accacgactt tttgtctcct tcgcacacac tgcttaactg cgctttcgcc aggtcgctga
    58441 cgcactgttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca
    58501 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
    58561 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
    58621 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
    58681 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
    58741 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
    58801 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
    58861 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc
    58921 tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaagcagg cggcagtacg
    58981 cctttggttt atccatttta tacaatccat gtaaaaaagg gccctgaaat tcaggaccct
    59041 ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt
    59101 aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg
    59161 tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc
    59221 ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac
    59281 agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc
    59341 acctggaacg gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta gttctgctca
    59401 atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg
    59461 ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc
    59521 tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg
    59581 cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga
    59641 tcccagttga taccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc
    59701 atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa
    59761 cccggaccgg ctttaaccac agcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt
    59821 gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc
    59881 agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt
    59941 ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta
    60001 tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct
    60061 gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg
    60121 ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggcc
    60181 tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt
    60241 gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc
    60301 aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc
    60361 tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg
    60421 tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca
    60481 aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcggct
    60541 ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg
    60601 atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag
    60661 gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg
    60721 gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc
    60781 cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga tttagtttac
    60841 cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc agcgcaaaga
    60901 cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa atcatgtagc cagacggcat
    60961 aggttttgcc cgtttcctca cctttacgat aggccgggcg cgggtcctgc gccagtactt
    61021 cgcggataaa cagcgttaac tgcggataac gcttctccag cgtcaaaagc tgcttttcga
    61081 cctctgcggt aaaactcacc gccatctctg cagctggcgc actttgcgca tagctggcac
    61141 tggcatcggg aagcgattcg gcaaagggga gatacggttt gatatccact accggcgtac
    61201 catcgaccag atccagactg ccgagcttca gaatcacgct gcctttatgg caaacaacct
    61261 ctttcagctc taccagcgac atgccaattg ggttagggcg gaaagtagag cgtgttgcga
    61321 aaacccccat tctggcgtta ccgccgaggc gcggtggacg cacagtcgga cgccagccgc
    61381 cttccatcgt ttgatgaaag acgaaaagga tccataaatg gctgaatgct tccaggccgc
    61441 gaacggcgtc ggcctggttg taggaagcaa tgagatgcag ttcgccgttg gcgcttttta
    61501 ccagacctgg ctggcgcgga acggcgaatt tttctttata gggcgagcga ataacgccta
    61561 tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc cgatacatac
    61621 agcctgacga tagcagcccg gcgtaccgct ggtgacttcg cagctatgca gtaatacagc
    61681 attggctttc attttagagg cattgatttg catctgctta cgtgcggttg gaatgctcgg
    61741 cggagagtcc tgattagagg cctggcaaga gtcgccactg acttcaccga gatcgcggaa
    61801 cggtttgccg actaattctt ctgcattggt ataaattcgg accggcgtgg cgcgcggcgc
    61861 tttcggtttt gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg gttcgacagg
    61921 ggatctgctt aacatggaac agccgcttag catgagtgct actaaacaga tcggtaaagc
    61981 acgcatagta tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg agcttgtaaa
    62041 aatgacaaga cgggcaagcg cccgtcctga atgatattac aaattgtgga aacagcctaa
    62101 aaattaccag cctttaacag cgccgccgtt aaacactttg tttgctgctt cgtaaacttc
    62161 atcagactga taagcctgga cgaatttctt cacgttctcg gcgtctttgt tatcttcacg
    62221 cgttacgatc aggtttacgt atggggagtc tttatcttcg acaaagatac cgtctttcgc
    62281 cggagtcagg ccaatctggc tggcataggt ggtattgata actgccagag cgatttgcgc
    62341 gtcgtccaga gaacgcggca gttgcggtgc ttccagttca acaattttca gattttttgg
    62401 gttctcaaca acatcaagaa cggtcggcag caggccaacg ccatctttca gtttgatcaa
    62461 gcccactttt tgcagcagca gcagtgaacg accaagatta gttggatcgt ttggcacggc
    62521 aacctgcgaa ccatcctgca gttcatccag tgatttgatt ttcttggagt aaccagcaat
    62581 cggataaaca aaagtgttgc ctactgcgac cagtttgtag ccacgatctt tcagttgctg
    62641 atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc tcaatgcttc
    62701 gtttggcaga acatagtcgt tgaaggttac cagctcaacg tccaggccat atttgtcttt
    62761 cgcaactttc tgcgcgactt ctgctacctg ctgttcggca ccaacaatca cgccgacttt
    62821 aatgtggttt ggatcttttt catcctgacc gcagcctacc agtgccagtg atccaatcag
    62881 ggctcccact gccgcaaagg ttttgaattt gaacgccata ccttattcct tcttcaatta
    62941 tttatgttgt gttgaacgtt acttgcgagt gacagcccgg acgatgcggt cgcctgcgaa
    63001 ctgaattaaa taaaccagaa tgaccagcaa taccagtacc gtattcatca ccgtcgcgtt
    63061 gtagccgatg tagccatact gatagccaat ctgacctaaa ccaccggcac cgactgcacc
    63121 acccatcgcg gaataaccga ccagggtaat cagggtgata gttgccgcat tcaccagacc
    63181 cggcaacgct tccggtaaca gcactttaca gacgatctgc attggcgtgg cacccattgc
    63241 gcgggaagct tcaattaacc cggttgggat ctccagcaga gcgttctcga ccatacgggc
    63301 aataaacggt gctgcaccaa cggttaacgg aacaattgct gcctgcaaac caatcgatgt
    63361 accgacaata acgcgggtaa acggaatcat ccataccagc aagataatga acgggatgga
    63421 acggaaaatg ttcacaatcg cagaaacggt acgatacagc ttcgcgttag caataatttg
    63481 ccccggacgc gtgacataaa gcagaacgcc aaccggcaga ccaatcacaa acccaaaaaa
    63541 tccggatacg aaggtcattg ccagcgtttc ccatacgcca cgaaccagca gccacatcat
    63601 cggctcagac ataacccagt acctctactt ttacatggtg ttcctgcagc caggcaatgg
    63661 cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag catgatgccg aatttaacgc
    63721 caccggcgta gtccatctgc gcgctaataa tgttgttgtt gacgttgaaa cgacgcgcgg
    63781 tttcagaaag cagtggggca tcgaccgatt gaccggtaaa ctccagacgc agcatcggca
    63841 cgcagtcagt aaatggctcc gtttgcagac gttcctggta atcttccggg atatccagat
    63901 gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt cggatgcgag aacacttcac
    63961 ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac cgccacgcaa tcacaaatac
    64021 gcttcacaac gtccatttcg tgggtgatca acagaatcgt caaacccaga cggcggttaa
    64081 tgtctttcag cagttcgaga atagaacgtg tcgttgccgg gtccagcgcg ctggtggctt
    64141 catcacacag caatactttg ggattgctgg ctaatgcacg ggcaatcgcc acacgttgtt
    64201 tctgcccacc ggaaagattc gacgggtagc tatcatgctt atcgccaaga ccaaccaatg
    64261 acagcaattc cgtcacgcga cgtttgatct cgtctttcgg tgtgttgtcc agctccagcg
    64321 gcagagccac gttgccaaaa acagtacgcg aagagagcag gttaaaatgc tggaaaatca
    64381 taccaatctg gcggcgagct ttggtcaact cggattctga cagcgtggtc aattcctggc
    64441 catcgaccag cacgctaccc tcggttgggc gctccagcag gtttacacaa cgtataagcg
    64501 tactcttacc cgcgcctgag gcaccgataa cgccataaat ttgtccagct ggcacatgca
    64561 ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg gtggaacact ttggtgatat
    64621 tcgaaagttt tatcattgat tatttattat cgtcattaag ttagtcgtag catctcgaat
    64681 gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag gcatccagac gtctaaatca
    64741 atcaggttta tgcgaagagc actttcttgc aggtcgaaac atgcgatact agcgtcccat
    64801 gccttattaa ggagctataa aaggtggcga agagcgtacc cgcaattttt cttgaccgtg
    64861 atggcaccat taatgtcgat cacggctatg tccatgagat cgacaacttt gaatttatcg
    64921 acggtgttat tgacgccatg cgcgagctaa aaaaaatggg ctttgcgctg gtggtagtaa
    64981 ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc acagtttgaa acgctgaccg
    65041 agtggatgga ctggtcgctg gcggaccgag atgtcgatct ggatggtatc tattattgcc
    65101 cgcatcatcc gcaggggagt gttgaagagt ttcgccaggt ctgcgattgc cgcaaaccac
    65161 atccggggat gcttttgtca gcacgcgatt atttgcatat tgatatggcc gcttcttata
    65221 tggtgggcga taaattagaa gatatgcagg cagcggttgc ggcgaacgtg ggaacaaaag
    65281 tgctggtgcg tacgggtaaa cctattacac ctgaagcaga aaacgcggca gattgggtgt
    65341 taaatagcct ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg gcgcaatgat
    65401 taaaagatga acggttgaaa taaaaatgca tttttccgct tgtcttcctg agccgactcc
    65461 ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac agccggttcg
    65521 gttgaagaga aaaaatcctg aaattcaggg ttgactctga aagaggaaag tgtgatatac
    65581 gccacctcgc gacagtgagc tgaaagctgc gtcgcaactg ctctttaaca atttatcaga
    65641 caatctgtgt gggcactcga agatacggat tcttaacgtc gcaagacga
//
LOCUS       DAJVRL010000007        65680 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVRL010000007 DAJVRL010000000
VERSION     DAJVRL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.007"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="OQN02_000446"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="OQN02_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1641371.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="OQN02_000447"
     CDS             complement(1150..1236)
                     /locus_tag="OQN02_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1641372.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OQN02_000448"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OQN02_000448"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1641373.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OQN02_000449"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OQN02_000449"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1641374.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="OQN02_000450"
     CDS             3585..3629
                     /locus_tag="OQN02_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641375.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OQN02_000451"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OQN02_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1641376.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OQN02_000452"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OQN02_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1641377.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OQN02_000453"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OQN02_000453"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1641378.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OQN02_000454"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OQN02_000454"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1641379.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OQN02_000455"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OQN02_000455"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1641380.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OQN02_000456"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OQN02_000456"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1641381.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OQN02_000457"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OQN02_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCS1641382.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OQN02_000458"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OQN02_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1641383.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OQN02_000459"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OQN02_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1641384.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OQN02_000460"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OQN02_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1641385.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OQN02_000461"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OQN02_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1641386.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OQN02_000462"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OQN02_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1641387.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OQN02_000463"
     CDS             complement(16981..17745)
                     /locus_tag="OQN02_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1641388.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OQN02_000464"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OQN02_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1641389.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OQN02_000465"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OQN02_000465"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1641390.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OQN02_000466"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OQN02_000466"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1641391.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OQN02_000467"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OQN02_000467"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1641392.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OQN02_000468"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OQN02_000468"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1641393.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OQN02_000469"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OQN02_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1641394.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OQN02_000470"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OQN02_000470"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1641395.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OQN02_000471"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OQN02_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1641396.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OQN02_000472"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OQN02_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1641397.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OQN02_000473"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OQN02_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1641398.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OQN02_000474"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OQN02_000474"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1641399.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OQN02_000475"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OQN02_000475"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1641400.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OQN02_000476"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OQN02_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1641401.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OQN02_000477"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OQN02_000477"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1641402.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OQN02_000478"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OQN02_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1641403.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OQN02_000479"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OQN02_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1641404.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OQN02_000480"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OQN02_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1641405.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OQN02_000481"
     CDS             complement(40421..41815)
                     /locus_tag="OQN02_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1641406.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OQN02_000482"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OQN02_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS1641407.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OQN02_000483"
     CDS             complement(42158..43444)
                     /locus_tag="OQN02_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1641408.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OQN02_000484"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OQN02_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OQN02_000485"
     CDS             complement(44451..45221)
                     /locus_tag="OQN02_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS1641409.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /gene="caiT"
                     /locus_tag="OQN02_000486"
     CDS             45693..47207
                     /gene="caiT"
                     /locus_tag="OQN02_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS1641410.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="OQN02_000487"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="OQN02_000487"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS1641411.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="OQN02_000488"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="OQN02_000488"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCS1641412.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="OQN02_000489"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="OQN02_000489"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS1641413.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..52247
                     /gene="caiD"
                     /locus_tag="OQN02_000490"
     CDS             51462..52247
                     /gene="caiD"
                     /locus_tag="OQN02_000490"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS1641414.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52253..52843
                     /gene="caiE"
                     /locus_tag="OQN02_000491"
     CDS             52253..52843
                     /gene="caiE"
                     /locus_tag="OQN02_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS1641415.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="OQN02_000492"
     CDS             complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="OQN02_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCS1641416.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53586..56807)
                     /gene="carB"
                     /locus_tag="OQN02_000493"
     CDS             complement(53586..56807)
                     /gene="carB"
                     /locus_tag="OQN02_000493"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1641417.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56825..57973)
                     /gene="carA"
                     /locus_tag="OQN02_000494"
     CDS             complement(56825..57973)
                     /gene="carA"
                     /locus_tag="OQN02_000494"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1641418.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="OQN02_000495"
     CDS             complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="OQN02_000495"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1641419.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="OQN02_000496"
     CDS             complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="OQN02_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1641420.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="OQN02_000497"
                     /gene_synonym="lytB"
     CDS             complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="OQN02_000497"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1641421.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="OQN02_000498"
     CDS             complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="OQN02_000498"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1641422.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="OQN02_000499"
     CDS             complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="OQN02_000499"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1641423.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="OQN02_000500"
     CDS             complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="OQN02_000500"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1641424.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65238..>65680)
                     /gene="ribF"
                     /locus_tag="OQN02_000501"
     CDS             complement(65238..>65680)
                     /gene="ribF"
                     /locus_tag="OQN02_000501"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCS1641425.1"
                     /translation="AVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTAN
                     VPLRRQVSPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYG
                     RHIQVVLRKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
BASE COUNT        15876 a        17900 c        16961 g        14943 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccagaaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaacataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa
    51661 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat
    51721 taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg
    51781 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    51841 tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc
    51901 cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    51961 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    52021 ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    52081 tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    52141 gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc attgaagggc
    52201 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    52261 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    52321 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    52381 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    52441 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcatgg
    52501 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtaat
    52561 tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc
    52621 aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    52681 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    52741 cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg aggaaaatcg
    52801 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc
    52861 tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc ggtatcaacg
    52921 agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    52981 agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc
    53041 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    53101 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    53161 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    53221 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    53281 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    53341 caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt
    53401 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    53461 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc
    53521 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca
    53581 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    53641 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    53701 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    53761 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    53821 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    53881 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    53941 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    54001 ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact
    54061 tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac
    54121 ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc
    54181 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    54241 gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact
    54301 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    54361 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc
    54421 agcgaacatg cggagtcacc ggagtgcaca cccgcctgct cgatgtgctc catgatgccg
    54481 ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca
    54541 aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag
    54601 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    54661 ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca
    54721 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt
    54781 tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg
    54841 gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    54901 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    54961 aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg
    55021 gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg
    55081 ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta
    55141 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca
    55201 aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc
    55261 agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag
    55321 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc
    55381 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    55441 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    55501 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    55561 tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt
    55621 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    55681 gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta
    55741 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    55801 atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    55861 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    55921 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    55981 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    56041 agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag
    56101 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    56161 tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc
    56221 agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta
    56281 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc
    56341 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    56401 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca
    56461 atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc
    56521 agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc
    56581 ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    56641 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    56701 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    56761 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    56821 ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg
    56881 gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggct
    56941 tatcgatgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    57001 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    57061 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    57121 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    57181 ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg
    57241 ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg
    57301 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    57361 tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    57421 tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    57481 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    57541 ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt
    57601 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat
    57661 ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    57721 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    57781 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    57841 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    57901 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    57961 ctgacttaat caaaacaccc tccagagaat actcactcac tttatttgca tattaattca
    58021 cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    58081 ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg
    58141 gctcatgcat gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa
    58201 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg
    58261 gtcaaaattt acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta
    58321 tagaaaattt aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa
    58381 ttaataaatt aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt
    58441 gagatcaagc acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc
    58501 cgatcttacc gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag
    58561 acgctcgcca atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac
    58621 ggtagcaaaa ccaatggtac ctggcacacg ttcaccggta tggccttcac gactgtagac
    58681 cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc
    58741 ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt
    58801 gtagtcaccc atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac
    58861 gctaaaattg gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt
    58921 accggcttcg tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa
    58981 agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc
    59041 ttttatcgca tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc
    59101 accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac
    59161 gccttctaat gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc
    59221 gatggcaacg cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac
    59281 cgttttcaga gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa
    59341 ttaatgataa acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc
    59401 gataacatgt gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc
    59461 cttttacatt cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga
    59521 tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg
    59581 gcttgagggt gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc
    59641 gcagaccgct ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc
    59701 cggtgcgatt caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg
    59761 tgacatccag accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt
    59821 ctggatcggc agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac
    59881 cgcccatgat caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca
    59941 gcgcaatatt ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca
    60001 gggcatcccg aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa
    60061 agtcgtagcc agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta
    60121 ccagcggcac agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt
    60181 gcagggcatt gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt
    60241 gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg
    60301 ggtcagtatc gaggaagata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa
    60361 gaccggcatt ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac
    60421 acgcagctct ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc
    60481 ttcaccacca cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc
    60541 cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc
    60601 tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg
    60661 gttggagttg gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag
    60721 ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc
    60781 gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga
    60841 cagcgtggtt tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac
    60901 atcgtctggc gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt
    60961 cccttccact tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact
    61021 ggcgcgggca acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt
    61081 caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat
    61141 caggatcgcg ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg
    61201 caagctatcg accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc
    61261 gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc
    61321 acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca
    61381 cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg
    61441 agtcgccgtt aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa
    61501 gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact
    61561 gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt
    61621 tgtcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc
    61681 ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt
    61741 cgagttttag cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc
    61801 aggtttgctc ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct
    61861 gtaggccgca tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc
    61921 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    61981 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    62041 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    62101 cgtggccttc gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc
    62161 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    62221 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    62281 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
    62341 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    62401 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
    62461 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    62521 gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag
    62581 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    62641 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc
    62701 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    62761 agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
    62821 taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca
    62881 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac
    62941 agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct
    63001 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    63061 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    63121 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    63181 gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    63241 tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg
    63301 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    63361 gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
    63421 tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa
    63481 gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg
    63541 gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg
    63601 tcgagagtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
    63661 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    63721 agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt
    63781 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    63841 atacgcgcct ggccccagtc cgggagccac tgcacgcctt tgatctcttt cagtgactgc
    63901 gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc
    63961 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc
    64021 gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca
    64081 tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    64141 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    64201 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    64261 cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga
    64321 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    64381 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    64441 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    64501 ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg
    64561 acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa
    64621 cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg
    64681 attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg
    64741 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
    64801 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc
    64861 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
    64921 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    64981 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    65041 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    65101 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    65161 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    65221 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    65281 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    65341 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    65401 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    65461 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    65521 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
    65581 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    65641 agagccagat tgtcatcagc cagcgcctga cgcacggcgg
//
LOCUS       DAJVRL010000008        65613 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVRL010000008 DAJVRL010000000
VERSION     DAJVRL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.008"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OQN02_000502"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OQN02_000502"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1641426.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OQN02_000503"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OQN02_000503"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1641427.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OQN02_000504"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OQN02_000504"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1641428.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OQN02_000505"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OQN02_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1641429.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OQN02_000506"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OQN02_000506"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1641430.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OQN02_000507"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OQN02_000507"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1641431.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OQN02_000508"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OQN02_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1641432.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OQN02_000509"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OQN02_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1641433.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OQN02_000510"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OQN02_000510"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1641434.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OQN02_000511"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OQN02_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1641435.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OQN02_000512"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OQN02_000512"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1641436.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OQN02_000513"
     CDS             complement(15665..16702)
                     /locus_tag="OQN02_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1641437.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OQN02_000514"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OQN02_000514"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1641438.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OQN02_000515"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="OQN02_000515"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS1641439.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="OQN02_000516"
     CDS             19444..19884
                     /locus_tag="OQN02_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS1641440.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OQN02_000517"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="OQN02_000517"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1641441.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="OQN02_000518"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="OQN02_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OQN02_000519"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="OQN02_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS1641442.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OQN02_000520"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="OQN02_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS1641443.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OQN02_000521"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="OQN02_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCS1641444.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OQN02_000522"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="OQN02_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS1641445.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OQN02_000523"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="OQN02_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS1641446.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OQN02_000524"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="OQN02_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS1641447.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OQN02_000525"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="OQN02_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS1641448.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OQN02_000526"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="OQN02_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS1641449.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="OQN02_000527"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="OQN02_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1641450.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OQN02_000528"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="OQN02_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1641451.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OQN02_000529"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="OQN02_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1641452.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OQN02_000530"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="OQN02_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1641453.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OQN02_000531"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="OQN02_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1641454.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OQN02_000532"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="OQN02_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1641455.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="OQN02_000533"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="OQN02_000533"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1641456.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="OQN02_000534"
     CDS             37412..37681
                     /locus_tag="OQN02_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1641457.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="OQN02_000535"
     CDS             complement(37684..38043)
                     /locus_tag="OQN02_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1641458.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="OQN02_000536"
     CDS             38184..38810
                     /locus_tag="OQN02_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1641459.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="OQN02_000537"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="OQN02_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1641460.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OQN02_000538"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="OQN02_000538"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1641461.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="OQN02_000539"
     CDS             41650..42315
                     /locus_tag="OQN02_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1641462.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="OQN02_000540"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="OQN02_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1641463.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="OQN02_000541"
     CDS             complement(42949..44166)
                     /locus_tag="OQN02_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1641464.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="OQN02_000542"
     CDS             44300..45349
                     /locus_tag="OQN02_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1641465.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="OQN02_000543"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="OQN02_000543"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1641466.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="OQN02_000544"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="OQN02_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1641467.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="OQN02_000545"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="OQN02_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1641468.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="OQN02_000546"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="OQN02_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1641469.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="OQN02_000547"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="OQN02_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1641470.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="OQN02_000548"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="OQN02_000548"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1641471.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="OQN02_000549"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="OQN02_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1641472.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="OQN02_000550"
     CDS             complement(51544..51903)
                     /locus_tag="OQN02_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1641473.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="OQN02_000551"
     CDS             complement(51900..52175)
                     /locus_tag="OQN02_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCS1641474.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="OQN02_000552"
     CDS             complement(52234..53358)
                     /locus_tag="OQN02_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1641475.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OQN02_000553"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="OQN02_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1641476.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="OQN02_000554"
     CDS             complement(56090..57157)
                     /locus_tag="OQN02_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1641477.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="OQN02_000555"
     CDS             complement(57523..59145)
                     /locus_tag="OQN02_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1641478.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="OQN02_000556"
     CDS             complement(59407..60996)
                     /locus_tag="OQN02_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1641479.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="OQN02_000557"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="OQN02_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="OQN02_000558"
     CDS             complement(62499..62975)
                     /locus_tag="OQN02_000558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641480.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="OQN02_000559"
     CDS             complement(62968..63732)
                     /locus_tag="OQN02_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS1641481.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="OQN02_000560"
     CDS             63978..64739
                     /locus_tag="OQN02_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1641482.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65346)
                     /locus_tag="OQN02_000561"
     CDS             complement(64846..65346)
                     /locus_tag="OQN02_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641483.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT        15937 a        17620 c        16161 g        15895 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agtggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaatatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgattcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gcc
//
LOCUS       DAJVRL010000009        60304 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVRL010000009 DAJVRL010000000
VERSION     DAJVRL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.009"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..322
                     /locus_tag="OQN02_000562"
     CDS             <1..322
                     /locus_tag="OQN02_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1641484.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="OQN02_000563"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="OQN02_000563"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1641485.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="OQN02_000564"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="OQN02_000564"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1641486.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OQN02_000565"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OQN02_000565"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1641487.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="OQN02_000566"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="OQN02_000566"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1641488.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="OQN02_000567"
     CDS             complement(3726..4955)
                     /locus_tag="OQN02_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1641489.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="OQN02_000568"
     CDS             complement(5019..5459)
                     /locus_tag="OQN02_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1641490.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="OQN02_000569"
     CDS             complement(5564..6334)
                     /locus_tag="OQN02_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1641491.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="OQN02_000570"
     CDS             complement(6331..7257)
                     /locus_tag="OQN02_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1641492.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVEHKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="OQN02_000571"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="OQN02_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1641493.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OQN02_000572"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OQN02_000572"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1641494.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="OQN02_000573"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="OQN02_000573"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1641495.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OQN02_000574"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OQN02_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1641496.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="OQN02_000575"
     CDS             12964..13311
                     /locus_tag="OQN02_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1641497.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="OQN02_000576"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="OQN02_000576"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1641498.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="OQN02_000577"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="OQN02_000577"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1641499.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="OQN02_000578"
     CDS             complement(15131..15493)
                     /locus_tag="OQN02_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1641500.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OQN02_000579"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OQN02_000579"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1641501.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="OQN02_000580"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="OQN02_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OQN02_000581"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OQN02_000581"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1641502.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OQN02_000582"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OQN02_000582"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1641503.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OQN02_000583"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OQN02_000583"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1641504.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OQN02_000584"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OQN02_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1641505.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="OQN02_000585"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="OQN02_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1641506.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OQN02_000586"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OQN02_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1641507.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OQN02_000587"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OQN02_000587"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1641508.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="OQN02_000588"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="OQN02_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1641509.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="OQN02_000589"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="OQN02_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1641510.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="OQN02_000590"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="OQN02_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="OQN02_000591"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="OQN02_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCS1641511.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OQN02_000592"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OQN02_000592"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1641512.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="OQN02_000593"
     CDS             complement(36348..36386)
                     /locus_tag="OQN02_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641513.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="OQN02_000594"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="OQN02_000594"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1641514.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="OQN02_000595"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="OQN02_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1641515.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="OQN02_000596"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="OQN02_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1641516.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OQN02_000597"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OQN02_000597"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1641517.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OQN02_000598"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OQN02_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1641518.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OQN02_000599"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OQN02_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1641519.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OQN02_000600"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OQN02_000600"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1641520.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OQN02_000601"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OQN02_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1641521.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OQN02_000602"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OQN02_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1641522.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OQN02_000603"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OQN02_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1641523.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OQN02_000604"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OQN02_000604"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1641524.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OQN02_000605"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OQN02_000605"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1641525.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OQN02_000606"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OQN02_000606"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1641526.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OQN02_000607"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OQN02_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1641527.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OQN02_000608"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OQN02_000608"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1641528.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OQN02_000609"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OQN02_000609"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1641529.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OQN02_000610"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OQN02_000610"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1641530.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OQN02_000611"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OQN02_000611"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1641531.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OQN02_000612"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OQN02_000612"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1641532.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OQN02_000613"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OQN02_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1641533.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OQN02_000614"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OQN02_000614"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1641534.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OQN02_000615"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OQN02_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1641535.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14439 a        16719 c        14826 g        14320 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccaag gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgtg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgaacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagcg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgcct attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ttccttgatg taaataaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactggac gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagaattg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggcaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcaa
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaaccgc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac agctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgactctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgc
//
LOCUS       DAJVRL010000010        59845 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVRL010000010 DAJVRL010000000
VERSION     DAJVRL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.010"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="OQN02_000616"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="OQN02_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1641536.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="OQN02_000617"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="OQN02_000617"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1641537.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="OQN02_000618"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="OQN02_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1641538.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OQN02_000619"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="OQN02_000619"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS1641539.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OQN02_000620"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="OQN02_000620"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1641540.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="OQN02_000621"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="OQN02_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1641541.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="OQN02_000622"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="OQN02_000622"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCS1641542.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="OQN02_000623"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="OQN02_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="OQN02_000624"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="OQN02_000624"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1641543.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="OQN02_000625"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="OQN02_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1641544.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OQN02_000626"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="OQN02_000626"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1641545.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="OQN02_000627"
     CDS             complement(13538..13933)
                     /locus_tag="OQN02_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1641546.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OQN02_000628"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="OQN02_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1641547.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="OQN02_000629"
     CDS             complement(15220..15447)
                     /locus_tag="OQN02_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1641548.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="OQN02_000630"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="OQN02_000630"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1641549.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="OQN02_000631"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="OQN02_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1641550.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OQN02_000632"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="OQN02_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1641551.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="OQN02_000633"
     CDS             complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="OQN02_000633"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1641552.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19689..19765
                     /locus_tag="OQN02_000634"
     tRNA            19689..19765
                     /locus_tag="OQN02_000634"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19723..19725,aa:Met,seq:cat)
     gene            19799..19875
                     /locus_tag="OQN02_000635"
     tRNA            19799..19875
                     /locus_tag="OQN02_000635"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19833..19835,aa:Met,seq:cat)
     gene            complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="OQN02_000636"
     CDS             complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="OQN02_000636"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1641553.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21435..22766
                     /gene="argA"
                     /locus_tag="OQN02_000637"
     CDS             21435..22766
                     /gene="argA"
                     /locus_tag="OQN02_000637"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1641554.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22828..24654)
                     /gene="recD"
                     /locus_tag="OQN02_000638"
     CDS             complement(22828..24654)
                     /gene="recD"
                     /locus_tag="OQN02_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1641555.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24654..28196)
                     /gene="recB"
                     /locus_tag="OQN02_000639"
     CDS             complement(24654..28196)
                     /gene="recB"
                     /locus_tag="OQN02_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1641556.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="OQN02_000640"
     CDS             complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="OQN02_000640"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1641557.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31253..34621)
                     /gene="recC"
                     /locus_tag="OQN02_000641"
     CDS             complement(31253..34621)
                     /gene="recC"
                     /locus_tag="OQN02_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1641558.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34634..34966)
                     /locus_tag="OQN02_000642"
     CDS             complement(34634..34966)
                     /locus_tag="OQN02_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1641559.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34951..35358)
                     /locus_tag="OQN02_000643"
     CDS             complement(34951..35358)
                     /locus_tag="OQN02_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1641560.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35355..35918)
                     /locus_tag="OQN02_000644"
     CDS             complement(35355..35918)
                     /locus_tag="OQN02_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1641561.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35909..36379)
                     /locus_tag="OQN02_000645"
                     /pseudo
     CDS             complement(35909..36379)
                     /locus_tag="OQN02_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="OQN02_000646"
     CDS             complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="OQN02_000646"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1641562.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="OQN02_000647"
     CDS             complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="OQN02_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1641563.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="OQN02_000648"
     CDS             complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="OQN02_000648"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1641564.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="OQN02_000649"
     CDS             complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="OQN02_000649"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1641565.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41864..42553
                     /gene="mutH"
                     /locus_tag="OQN02_000650"
     CDS             41864..42553
                     /gene="mutH"
                     /locus_tag="OQN02_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1641566.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42622..43335
                     /locus_tag="OQN02_000651"
     CDS             42622..43335
                     /locus_tag="OQN02_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1641567.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43473..43691
                     /gene="ygdR"
                     /locus_tag="OQN02_000652"
     CDS             43473..43691
                     /gene="ygdR"
                     /locus_tag="OQN02_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1641568.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43799..44839
                     /locus_tag="OQN02_000653"
     CDS             43799..44839
                     /locus_tag="OQN02_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1641569.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="OQN02_000654"
     CDS             complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="OQN02_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1641570.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46055..48214)
                     /gene="aas"
                     /locus_tag="OQN02_000655"
     CDS             complement(46055..48214)
                     /gene="aas"
                     /locus_tag="OQN02_000655"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1641571.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48800..49831
                     /gene="galR"
                     /locus_tag="OQN02_000656"
     CDS             48800..49831
                     /gene="galR"
                     /locus_tag="OQN02_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1641572.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="OQN02_000657"
     CDS             complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="OQN02_000657"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1641573.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51222..52157
                     /gene="lysR"
                     /locus_tag="OQN02_000658"
     CDS             51222..52157
                     /gene="lysR"
                     /locus_tag="OQN02_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1641574.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="OQN02_000659"
     CDS             complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="OQN02_000659"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1641575.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52965..54383)
                     /gene="araE"
                     /locus_tag="OQN02_000660"
     CDS             complement(52965..54383)
                     /gene="araE"
                     /locus_tag="OQN02_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCS1641576.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="OQN02_000661"
     CDS             complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="OQN02_000661"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1641577.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="OQN02_000662"
     CDS             complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="OQN02_000662"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1641578.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56612..57793)
                     /locus_tag="OQN02_000663"
     CDS             complement(56612..57793)
                     /locus_tag="OQN02_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1641579.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58048..59277
                     /locus_tag="OQN02_000664"
                     /pseudo
     CDS             58048..59277
                     /locus_tag="OQN02_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14775 a        16054 c        14575 g        14441 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 ctggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gataaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagcctgg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatc attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttccaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatgacgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccaaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgtcggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgctgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgagt
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat agcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcagggggc ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcaccctgtc atatccgtaa
    18361 aaacggcata cagaatatca catcagccgc taaagacgtt acctgcgccc ggagccgttt
    18421 tcagcaccca aacccgtcca tagtggttgt accaacctgc gcggtgtccg gcttccggcc
    18481 cgatcccttg atagatatcg aagtgttggc ctttgattgc gcccccgaca tccaacgcca
    18541 ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc agcggcactt
    18601 ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc ggaggcaaca gaagcacggc
    18661 caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt ttaaagaaga
    18721 cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt gtttcgcccc
    18781 aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg tcgatcagca
    18841 ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa aagttaagcg
    18901 gactgccatc accaaagtcg atatacccac taccctgcac atccataatg aagttatcca
    18961 tcagagagtt gctgtaagcg aggatgtatt tatcgcccaa tgccccggcg tagatctccg
    19021 cacgagacgg cagacgacca cgttttggcg gcatacggta aataggatac tggaactcgc
    19081 cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac tgcacgttac
    19141 catagttgtc ggcaccttcc atctgccagg catcaatgcc gaactggcgc atattgcggg
    19201 tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc tggttgccat
    19261 acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca ccggcgttaa
    19321 tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta aatttcccgt
    19381 ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc attgccacaa
    19441 ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc ttcaggttaa
    19501 aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa tgaaatgagc agcctctccc
    19561 tgacgcaaat tttgcacaaa aaataggctt tagtggtttg tttttgttca aaatcatgct
    19621 aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt atagtgcgca
    19681 tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    19741 ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcag tacggtgacg
    19801 cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtc ggttcaaatc
    19861 cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc tgatctccca
    19921 taaaaaaagc gccattcagc gcctttttat catccccttc tcgccagcgt tgccccatcg
    19981 gcaaaatacg ctttaatccc cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc
    20041 gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac cagaatggag
    20101 ggaatatctg gtgcctttag tacggcaaac ccggcctgtt caacttgatt tttatgcagc
    20161 ttgttgattt ttccgagctt attcagtacc gctttaccaa acttaaggct gtcagcaata
    20221 gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc gcttttgctc
    20281 acgccaccaa tcaagtccga ggcgttctgg gtttgtgcca gatattttgc cgcagtactg
    20341 gttgcgcctt tggttgagag cgcaaacacc gaggaaccgc tcggctgacg actggtaaag
    20401 gcgtcggcat ggatagagac aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc
    20461 acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat attgccctct
    20521 tcctcgatca gagagcgcaa acggcgagct atttgcaata ccacatcttt ttcgcgcgtt
    20581 ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag cataatgaca
    20641 atcggacgat cgcgccctgc tttacccggt tgtggaccac tttgcgctgg cggcacctgc
    20701 ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc
    20761 tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc gaccggcgca
    20821 agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatgcg tacggtttgc
    20881 gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc
    20941 gccatcccct tgagtaccga gttcaggttt acatcttcga tatccaccac cacgcgttca
    21001 gggttactca acgcgaactg cttatatttc agctgacgat ttgattctac cgtcacgcgg
    21061 gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctaaccgc agccaggctg
    21121 acctgactta cgctcaatag ccacatagcg cccgcgcctt gcagtaaacg acgacggctg
    21181 attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca
    21241 agtaaacgac taatttgacc ggaaacttta gcgaaagacg cataatctgt catctaataa
    21301 acggtaaaca ttctttttat attcacggca ttactgataa aaaagtcgct ctcgcataaa
    21361 atttacactt gcaccctgcg aaaaaacaga ataaaaatac aataatttca aataatcatg
    21421 caaaggggtg tgccgtggta aaggaacgta aaaccgagct ggtcgaggga ttccgccatt
    21481 cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc ggcggtgaag
    21541 ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg cacagcctcg
    21601 gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc
    21661 atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgccaaa acactggaac
    21721 tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg tcaatgagtc
    21781 tcaataacac gccgctgcag ggcgcgcata tcaacgtcgt tagtggcaat tttattattg
    21841 cccagccgct gggcgtcgat gacggcgtgg attactgcca tagcgggcgt atccggcgga
    21901 ttgatgaaga cgcgctccat cgtcaactgg aaagcggtgc aatagtgcta atggggccgg
    21961 tcgctgtttc agtcactggc gagagcttta atctgacctc ggaagagatt gccactcaac
    22021 tagccatcaa actgaaagct gaaaagatga ttggtttttg ctcttcccag ggcgttacta
    22081 atgacgacgg tgatattgtc tccgaacttt tccctaacga agcgcaagca cgggtagaag
    22141 cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga
    22201 aagcctgccg cagcggcgtg cgccgctgtc atttaatcag ttatcaggaa gatggcgcgc
    22261 tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg gaaagcgccg
    22321 agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg attcgcccgc
    22381 tggagcagca aggtattctg gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca
    22441 aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc tatccgttcc
    22501 cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac cgcagttcat
    22561 cacggggcga ggttctgctg gaacgcattg ccgctcaggc gaagcagagc ggcttaagca
    22621 aattgtttgt gctgaccacg cgcagtcttc actggttcca ggaacgtgga tttaccccgg
    22681 tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag cgtaaatcca
    22741 aagtgttgat ggcggattta gggtaagcga tgaaaatcac cggatgcggc aggcgtaaca
    22801 ctcgtgcgtc gcatccggca attacgttta ttcccgcgaa ctaaacaacg ccgccagacc
    22861 actgcgccgc tcagtacgag tggcgattgc cgcacttaat atacgctcat cggcatacag
    22921 cgacagacga cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg ttactaccgg
    22981 cgtgcgttgg ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg
    23041 taccgtcatc gcccacgtag tttcgtgctc tggcaagcga ctcggttgca cagacttaat
    23101 attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc
    23161 gatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ccggtcggcc
    23221 ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaactgttc
    23281 aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagctg
    23341 gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag
    23401 ataacgtccg taacccgcaa gagcttcctc aagcatcgca atataatctt cgccgctctg
    23461 taaaagtcgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt
    23521 atcaccacgg ttgatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata
    23581 gcttttttgc agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc
    23641 cggaacgtga gtccccgtca ggcggcttag ctgcctggca cgctcggcgg taaagcccgc
    23701 gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc
    23761 acgatcgccg agaaagatca ctcgcgcatg atctggcaag gcgtcgatca gtctcgacat
    23821 cataggcaga tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt
    23881 accggcatga tgacgtaaac gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt
    23941 gctggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc
    24001 cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat
    24061 acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc agcaacttcg ccacggtggt
    24121 cgttttaccg gtgcctgggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc
    24181 cgctgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg
    24241 cgccagtaga gcttcatcaa cctcaatggt atgattcacc tcattgaaga agcgcgctac
    24301 cgtccgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg
    24361 cgtgggttca tctcccctgc tgaccgcttg agaagccagc aagcattctt cccaattttg
    24421 tagctcaccg atttcactga cacaggtcgc caacagcgga ttcgacgcct cgttattttc
    24481 cagtcgtgaa agcggcaaac agacgtgtcc ctctccggca tcatgactta acagtgccgc
    24541 cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag
    24601 cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc
    24661 tcctccaggg tcataccggc aaacatctca tccatcaggg caatcaaccc ggcgttgggt
    24721 cgggttgtgt aaatcccttg ttgcggatgt tctttatcaa cgccacgcag gaacagatag
    24781 ataacgccgc caaagtgaag atcatagtcg taatcagcaa tgcgatggcg cagataacga
    24841 tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg catcgctgct
    24901 gccatagcct gttgggtgta agccgaactg tcttcaccca gccagttgga tttatagtcg
    24961 agcaggtaat aacgcccttc gtggcggaat accaggtcga taaagccttt taacatgccg
    25021 cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt
    25081 agtgcatcaa gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc
    25141 acctgtttat cgcggtcgga aagctgactc aggctaacac ccgtttcatt gagaggtgcc
    25201 tggaggacag ccgtgatcca ctcggtcaac accggttccc actgcgattc aaagccgccg
    25261 agttccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca
    25321 aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac
    25381 gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga
    25441 tcctgagcga taccgtgacc acgctgctgc aagccggagt agctggtgac acgccagtta
    25501 tcgccgggca atcgttgtag tgtcctcgca ttcagttctg cagttaaagc atcattaacc
    25561 tgccagggct ggttatcacc agtttgtgcc gtttgccagg caatatcatc atcgcataac
    25621 gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc
    25681 ccgagcgcac tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc
    25741 ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc
    25801 agatcttccg ccagacgttc gacctccgcg aggtcgacgc tttctggcgc agcattaaga
    25861 tccaaaactg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa
    25921 ttggtaataa atggcaacca gaccagcgga tattccagcc ctttcgattt atggatcgtg
    25981 acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga ggcattactg
    26041 tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc
    26101 gttccggctt cttgaagcag ttcgctgata tgcaagatat cggtaagacg ccgctcaccg
    26161 cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc
    26221 ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc gactaccgca
    26281 tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt
    26341 gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac
    26401 aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa
    26461 acggaaggga tttccagcaa cgttaaggca tcgcgcacct gggcggcctc ctggcggctg
    26521 cgcaccagca cgctgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc
    26581 aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc
    26641 gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc
    26701 atcgcaggct gcgtttcacc tttaaataca aaacgtaacg cctgattttt cccggctgat
    26761 ttcactggaa taaacggtat ttcgcgaaac atgaacgtgt catcagtctg gctgaaaagc
    26821 ttgttcacac tgttcaccat tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg
    26881 gcgtgaactt cactacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat
    26941 atggcctgct tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata
    27001 cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc
    27061 gggaatcgcg tacggatcgc cgctgccaac acctcgccgc tttcgctacg cagcgcggaa
    27121 tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct
    27181 actgtttcgc ggatctcagc caatgcgcgg gtgatcaaca gatcgcggat cgacaatggt
    27241 tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggcc
    27301 ttcgtgcgat cttctaagaa acgctgggag aatttttcca gcgactccgg caactgataa
    27361 ctgtttgtct cttcttctgc ccaggcgctg atcttttcga tccatttagc ctgattgcta
    27421 cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact
    27481 gcgtcgcgcc actgctgttt taccgtatca atacgcgcca caatttgcgc gtggcgggaa
    27541 gccagcgttt catcatcggg cggcggtgct ttgataaccg gcggttcgcc ttgcagataa
    27601 cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac gacctgggct
    27661 atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt
    27721 agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc
    27781 aggttgagca tgcgctggca aaagccgtga atagtaaaga ctgccgcttc atccatctgc
    27841 cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg
    27901 cgttcgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata
    27961 ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt gaccaccagc
    28021 agttcttcaa cgatcagcgg gcggggaaag gcggcggaac cgcctagtcc aagtaacagg
    28081 cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg
    28141 cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt
    28201 ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc
    28261 ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg acagaatagc
    28321 catgccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag
    28381 cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa
    28441 atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt
    28501 aattaccgcc tgctggattt gcgcaaactc atctggcttc atcgctcgca attttgcctc
    28561 tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct
    28621 ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc
    28681 atagcccaat tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc
    28741 caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc
    28801 tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc
    28861 gaccactaca tctttgtttc gacaccactc tgaaccatca gcgcccaact gtttttgcac
    28921 atcgcgtgcc agcgttgttg cctgggcctc ggtcatgttg ccgataacca taaactctgg
    28981 tcgagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg
    29041 caaaatttta cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg
    29101 cataatcgcc tgctcaaacg ctttaccctt ttctgcggaa tccatcatct ggttatacca
    29161 ggacttcgcc tgctcaagct gctcttccgt agcggtatag ctaaagtacc cctcgagcaa
    29221 tgcctggaac agctgcggca gacgctgggt gtaaccatta gcattaatca taaggccgtt
    29281 gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc tggttgctta actgatcaag
    29341 cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat
    29401 ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcgctgg caaaataacg
    29461 gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcagctctg gatggtcgta
    29521 tttcttctct gacttaatca gcgagaaatc atcaggaata taagggttaa gctctggcaa
    29581 agagagcgca atgtcggcgg cttttttctg ccagtcggcg aaagtttgtg cgctgatttt
    29641 atcgacctga tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct
    29701 gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttcct ttactgcttt
    29761 agcatcgtac cgatcggcaa tattgactgc atccagcgta tgctcaacag gaacgcgaat
    29821 catggtatct gccagccatt cgacgtaatc catatcacgg gtgatcgacg gataacggaa
    29881 gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcaatgc ctttttcacg
    29941 taacagattg agatagctaa aaattgccgc cacaacctga tcgcgattag ccaagccttt
    30001 atcagttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga
    30061 gttggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcagaaa gtgtacctgg
    30121 gctgcgattg ccaatcagat aggtaatcaa ttcatcggtt ttactacgga acttcgctga
    30181 gttgttatcg atgcgaaact caacgcgcaa cactttacgc ggcagcgcag ggacgtaatg
    30241 aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct
    30301 ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg
    30361 tttattactg taaataaccg ccttcatcaa attggcggaa tagtacttct cgtggaaatc
    30421 tttcagcgcc tgctgcaccg gattaccagg tttgtcgctt aaagtttcga ggttaccacc
    30481 agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg
    30541 catcccgtca cgcgtacgcg ccatggttaa ttcagcgttc actgcattac gctcacgttc
    30601 ggcgtatttc ttgtcgagca agggttctgc aatagcatcg gccaggcggt ctaccgcacc
    30661 gggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggcg cagtgctggc
    30721 attgtgacta ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt
    30781 tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg
    30841 atcttccagc gacccaacgg gcactaccag cgccgagagc gatttaactg cctgcggatc
    30901 agaaaccagc aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc
    30961 tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa
    31021 tggtgcccag agggcaacaa acaacaataa tgctttgaac caggtgctgc ggggcattca
    31081 cggacctcat aatcaacttt attttctgga cagattcaac aggttaacgc aaatcatctg
    31141 ccagaattta atcttgtgct gcacgagtca gcctatgttt atataaccat cagtccgtga
    31201 ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacaac actcatgact
    31261 gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgatg gcctccattg
    31321 tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca
    31381 ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc aacgtggaat
    31441 cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac
    31501 tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccctca atcagttgtg
    31561 agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactca ccgtctttgc
    31621 gtagaaaaag gcgactttcg ccattaccac cgctggcaca gtagacaagg tgttccagcc
    31681 aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg
    31741 gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca
    31801 tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcctggc
    31861 actgtgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga
    31921 agcggcggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat
    31981 tgatttgata acggctaagt ccttccagaa taaatggctc ggtgtctggg atttcgctgt
    32041 cttcagtatg aaagttcacc tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc
    32101 agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa
    32161 caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc
    32221 tttgtcgttc gcctggctgg tagttttgcg gatcaaacgg catccgggta tggaggcaag
    32281 taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta
    32341 gataatgact ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt
    32401 cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa
    32461 ttaacgcttc caggaacagg tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg
    32521 gtttctggct catcaggtca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt
    32581 tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa
    32641 tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccaga cgctgtgcca
    32701 gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg
    32761 cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg
    32821 gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttct agcggacgct
    32881 cctgtgccag cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca
    32941 ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct
    33001 gcgcgctctc catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt
    33061 gtccggtggc ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc
    33121 cggattcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg
    33181 ccgccagcac cggcacatcc agcagcgcca gcacatcctc tgacacaaag cgactgtcag
    33241 gcagtgataa caggctgata aacgcttcca gtaccggatg tgactgccgc gcacgacggt
    33301 cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacact gcctgaataa
    33361 acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg
    33421 gggcttcctc cagcatcgcc agcaagcgat cgtgtaaaac ttcaacttca cgctgcgggc
    33481 tatggcaaac gtggaatgtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc
    33541 tacgggaaaa ctcttcgatg ttcacacccg caacggcgcg gttttccagt tccagaatgt
    33601 cagactgaat gttatgcagc aggttatctg gcgtcacatc gacaaaagcg tccagctcct
    33661 ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttc ccccatgagg
    33721 ccagcagcga gttgccgaca tcctgttcac catcgctgtt aaagagctgt ccggcatttt
    33781 cgctatcgcg aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc tgacgagtca
    33841 gcagtttcgc cagataagca ggatctttaa tatcgcccca gtaataacgg caggggttgg
    33901 taaacaggag atggatttca atatgtttac ccagcgcctg gagcgcctgg agataaacag
    33961 gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg
    34021 tcgcggactc cagcgtttcg ataaagcgct gatagagatt cgcgcggtgc cagcgcggtt
    34081 gtccgagttc atgggtatat tccaccagcg ccttccacaa cggggcttgc caggcctgtg
    34141 cttctcccag cccttcaacc aaatgtcctg tttcccactg tgccagccag tccggacgat
    34201 agaccagata ctggtcaaac aggtccgccg cttttgagga aagctggaac agttttcgct
    34261 tgtcgctatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccagcaatt
    34321 gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg
    34381 ggatttccgg taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat
    34441 caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac
    34501 cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga
    34561 caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca
    34621 tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
    34681 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
    34741 ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat
    34801 gccgccagag ctgttggtac tggtttctgc tggcgagact gttcattgat gttcgctgga
    34861 tacccgataa cgcagtgaca atcatcacca tcagcaccat cagcaccatc gcaaccatta
    34921 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    34981 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    35041 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    35101 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    35161 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    35221 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    35281 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    35341 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    35401 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    35461 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    35521 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    35581 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    35641 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    35701 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    35761 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    35821 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    35881 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    35941 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    36001 agcgaatatg cccggcccat gcggtattac gcaggccaaa gaaagcaagc gaaggtgtca
    36061 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    36121 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    36181 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    36241 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    36301 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    36361 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    36421 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    36481 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    36541 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    36601 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    36661 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    36721 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    36781 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    36841 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    36901 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    36961 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    37021 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    37081 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    37141 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    37201 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    37261 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    37321 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    37381 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    37441 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    37501 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    37561 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    37621 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    37681 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    37741 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    37801 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    37861 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    37921 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    37981 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    38041 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    38101 gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc
    38161 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    38221 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    38281 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    38341 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    38401 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    38461 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    38521 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    38581 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    38641 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    38701 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    38761 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg
    38821 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    38881 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    38941 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    39001 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    39061 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    39121 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    39181 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    39241 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    39301 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    39361 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    39421 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    39481 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    39541 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgagccatct cgcacgacaa
    39601 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    39661 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    39721 gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta
    39781 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    39841 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    39901 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    39961 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    40021 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    40081 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    40141 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    40201 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    40261 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    40321 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    40381 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    40441 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    40501 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    40561 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    40621 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    40681 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    40741 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    40801 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    40861 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    40921 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    40981 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    41041 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    41101 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    41161 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    41221 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    41281 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    41341 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    41401 cattctgaat aagcccgata tagaaatttt aaaatattca ttattagtca atgcattaca
    41461 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    41521 ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact
    41581 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    41641 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    41701 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    41761 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    41821 cggcacataa ttactgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    41881 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    41941 ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga atttaaaacg
    42001 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    42061 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    42121 tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta atagcggggt
    42181 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    42241 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    42301 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    42361 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    42421 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    42481 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    42541 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    42601 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    42661 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    42721 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    42781 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    42841 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    42901 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    42961 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    43021 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    43081 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    43141 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    43201 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    43261 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    43321 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    43381 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    43441 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    43501 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    43561 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    43621 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    43681 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    43741 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    43801 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    43861 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    43921 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    43981 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    44041 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    44101 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    44161 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    44221 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    44281 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    44341 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    44401 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    44461 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    44521 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    44581 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    44641 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgct gtcaaccgtt
    44701 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    44761 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    44821 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    44881 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    44941 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    45001 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    45061 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    45121 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    45181 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    45241 cttcgctgcc gcacctgcgc caaccacgat accaatcact accatcgcgt tgagataggt
    45301 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    45361 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    45421 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    45481 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    45541 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    45601 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    45661 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    45721 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    45781 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    45841 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    45901 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgtca gagtggcaaa
    45961 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    46021 cttcgaccac aacgaagtgt taatgtgcac tgactcactc atcgtgttgt tccgcttcgt
    46081 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    46141 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    46201 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    46261 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    46321 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    46381 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    46441 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    46501 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    46561 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    46621 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    46681 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    46741 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    46801 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    46861 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    46921 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    46981 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    47041 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    47101 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    47161 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    47221 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    47281 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    47341 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    47401 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    47461 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    47521 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    47581 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    47641 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    47701 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    47761 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    47821 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    47881 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    47941 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    48001 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    48061 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    48121 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    48181 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    48241 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    48301 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    48361 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    48421 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    48481 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    48541 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    48601 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    48661 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    48721 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    48781 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    48841 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    48901 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    48961 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    49021 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    49081 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    49141 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt
    49201 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    49261 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    49321 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    49381 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    49441 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    49501 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    49561 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    49621 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    49681 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    49741 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    49801 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    49861 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    49921 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    49981 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    50041 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    50101 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    50161 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    50221 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    50281 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    50341 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    50401 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    50461 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    50521 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    50581 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    50641 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    50701 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    50761 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    50821 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    50881 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    50941 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    51001 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    51061 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    51121 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    51181 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    51241 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    51301 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    51361 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    51421 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    51481 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    51541 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    51601 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    51661 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    51721 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    51781 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    51841 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    51901 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    51961 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    52021 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    52081 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    52141 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    52201 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    52261 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    52321 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    52381 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    52441 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    52501 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    52561 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    52621 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    52681 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    52741 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    52801 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    52861 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    52921 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    52981 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    53041 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    53101 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    53161 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    53221 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    53281 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    53341 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    53401 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    53461 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    53521 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    53581 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    53641 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    53701 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    53761 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    53821 gctggtaaag caagaccccc caacattgcg cgccagttac cgctataact gaacgctgta
    53881 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    53941 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    54001 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    54061 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    54121 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    54181 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    54241 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    54301 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    54361 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    54421 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    54481 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat
    54541 tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt
    54601 gatatggatt atttcataac catgatatgg attatgatga tctagagata taaaaaaccc
    54661 tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat
    54721 ccacagcaat ggcataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg
    54781 gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg
    54841 cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag
    54901 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac
    54961 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    55021 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    55081 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt
    55141 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    55201 caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    55261 gcagatcggc ggtcaggctt aaaaaacgac gcccctgcgc cgtgacctgc tcgatggttt
    55321 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    55381 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag
    55441 aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc
    55501 tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat
    55561 aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc
    55621 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    55681 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
    55741 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    55801 acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
    55861 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    55921 cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc
    55981 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
    56041 gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    56101 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    56161 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    56221 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
    56281 cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa
    56341 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    56401 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat
    56461 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    56521 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    56581 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    56641 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    56701 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    56761 cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
    56821 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
    56881 agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg
    56941 gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc
    57001 gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat
    57061 tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg
    57121 cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt
    57181 ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt
    57241 tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca tattcgcgag
    57301 ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac aacccatcat
    57361 gcaccagact gtcaaccaac tggctattgc caagctgtgc accggtgcgg ctatcagtca
    57421 gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata tcagcctcgc
    57481 cacactgtat cgcctgagta gccagatgca gtgctttaag accggaaccg caaacgtcat
    57541 taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt aatagccgat tgccttgccg
    57601 gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg
    57661 caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg
    57721 aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca
    57781 caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt
    57841 acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca
    57901 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagatgggt
    57961 agatagggcg ttaatctccc atacttaact tggtttatgg taaattgccc tccattttgt
    58021 ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg
    58081 tgaagtttcg cgctctatgg cacagccgtt gatgcaggca cgctattcct tcctattcag
    58141 ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca
    58201 tattggccac ataaagcctt atgccagttc atcctctcat caaaaacatc agcaggtgaa
    58261 gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct gattaccacg
    58321 ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca
    58381 cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt
    58441 ctgggtgttg tattaattct gaatctcatt tttctgatgg gacgtcatgc cactattcgg
    58501 gtaatgggat ttttggtatt cccattgatt gcctatttct tatttctttc catttacctt
    58561 gtcggtagtt ggcaacctga tctattaaca acccaggtag agttcaatca gaataccctt
    58621 caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac gcccattatt
    58681 tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa
    58741 aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt tgtctttagc
    58801 tgcctgcttt ctattccatc ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt
    58861 ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc cgggattatt
    58921 gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt tattgaaggt
    58981 gccacagagg tcgtcaaaac aacactacag caggttggtg taaagaaaag tcgtgcattt
    59041 aaccacgcac tatcaattat gttggtatcg ctgattacct tcattgtttg ttgcattaac
    59101 ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat gatacttttc
    59161 atcatgccta cgctgtcaac gtatctcatc ccggcgctta aaccctggcg ttccatcgga
    59221 aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt tagctaatat
    59281 ccgttgtgga tggttcaaaa ttgctacaat tttgaaccat tccatttttc ataatattca
    59341 tttgttatta ttccgcccta tcacttctct cgtcatatcc tagaattatc agttagttaa
    59401 acacacaaag agatctgaac atcttaaata agactattta agatgcataa cttagattcg
    59461 caagatatct tctggtcatt caaaaacaat ttgcgatata gatattctca taccatgaga
    59521 tagttatctg gaccacgttc gaccatgcaa gattaaatca gtgaaacata atcatattta
    59581 ataacgagaa atgcattttt aaatatctta atcagaatta tagcaaatac agattaatcg
    59641 caatttttcc cagcaaaatt acaaaatcaa ggtgtattga catcatcaat caccaatgaa
    59701 attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa
    59761 agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt
    59821 ttgcagtact gcgcaaactc gtgtt
//
LOCUS       DAJVRL010000011        58576 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVRL010000011 DAJVRL010000000
VERSION     DAJVRL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.011"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..1103)
                     /locus_tag="OQN02_000665"
     CDS             complement(<1..1103)
                     /locus_tag="OQN02_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCS1641580.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALV"
     gene            complement(1315..1662)
                     /gene="pspE"
                     /locus_tag="OQN02_000666"
     CDS             complement(1315..1662)
                     /gene="pspE"
                     /locus_tag="OQN02_000666"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1641581.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1705..1926)
                     /gene="pspD"
                     /locus_tag="OQN02_000667"
     CDS             complement(1705..1926)
                     /gene="pspD"
                     /locus_tag="OQN02_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1641582.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1935..2294)
                     /gene="pspC"
                     /locus_tag="OQN02_000668"
     CDS             complement(1935..2294)
                     /gene="pspC"
                     /locus_tag="OQN02_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1641583.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2294..2518)
                     /gene="pspB"
                     /locus_tag="OQN02_000669"
     CDS             complement(2294..2518)
                     /gene="pspB"
                     /locus_tag="OQN02_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1641584.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2572..3240)
                     /gene="pspA"
                     /locus_tag="OQN02_000670"
     CDS             complement(2572..3240)
                     /gene="pspA"
                     /locus_tag="OQN02_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1641585.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3407..4384
                     /gene="pspF"
                     /locus_tag="OQN02_000671"
     CDS             3407..4384
                     /gene="pspF"
                     /locus_tag="OQN02_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1641586.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4504..5769)
                     /gene="puuE"
                     /locus_tag="OQN02_000672"
     CDS             complement(4504..5769)
                     /gene="puuE"
                     /locus_tag="OQN02_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCS1641587.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5807..7087)
                     /gene="puuB"
                     /locus_tag="OQN02_000673"
     CDS             complement(5807..7087)
                     /gene="puuB"
                     /locus_tag="OQN02_000673"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS1641588.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7089..8576)
                     /gene="puuC"
                     /locus_tag="OQN02_000674"
     CDS             complement(7089..8576)
                     /gene="puuC"
                     /locus_tag="OQN02_000674"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCS1641589.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8726..9283)
                     /gene="puuR"
                     /locus_tag="OQN02_000675"
     CDS             complement(8726..9283)
                     /gene="puuR"
                     /locus_tag="OQN02_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1641590.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9310..10074)
                     /gene="puuD"
                     /locus_tag="OQN02_000676"
     CDS             complement(9310..10074)
                     /gene="puuD"
                     /locus_tag="OQN02_000676"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS1641591.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10286..11704
                     /gene="puuA"
                     /locus_tag="OQN02_000677"
     CDS             10286..11704
                     /gene="puuA"
                     /locus_tag="OQN02_000677"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1641592.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11853..12017
                     /locus_tag="OQN02_000678"
     CDS             11853..12017
                     /locus_tag="OQN02_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641593.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12007..13392
                     /gene="puuP"
                     /locus_tag="OQN02_000679"
     CDS             12007..13392
                     /gene="puuP"
                     /locus_tag="OQN02_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1641594.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13526..13771
                     /locus_tag="OQN02_000680"
     CDS             13526..13771
                     /locus_tag="OQN02_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1641595.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14084..15727
                     /gene="sapA"
                     /locus_tag="OQN02_000681"
     CDS             14084..15727
                     /gene="sapA"
                     /locus_tag="OQN02_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1641596.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15724..16689
                     /gene="sapB"
                     /locus_tag="OQN02_000682"
     CDS             15724..16689
                     /gene="sapB"
                     /locus_tag="OQN02_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1641597.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16676..17566
                     /gene="sapC"
                     /locus_tag="OQN02_000683"
     CDS             16676..17566
                     /gene="sapC"
                     /locus_tag="OQN02_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1641598.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17566..18558
                     /gene="sapD"
                     /locus_tag="OQN02_000684"
     CDS             17566..18558
                     /gene="sapD"
                     /locus_tag="OQN02_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1641599.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18560..19366
                     /gene="sapF"
                     /locus_tag="OQN02_000685"
     CDS             18560..19366
                     /gene="sapF"
                     /locus_tag="OQN02_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1641600.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19434..19787
                     /locus_tag="OQN02_000686"
     CDS             19434..19787
                     /locus_tag="OQN02_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1641601.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20155..20943
                     /gene="fabI"
                     /locus_tag="OQN02_000687"
     CDS             20155..20943
                     /gene="fabI"
                     /locus_tag="OQN02_000687"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1641602.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21087..22214
                     /locus_tag="OQN02_000688"
     CDS             21087..22214
                     /locus_tag="OQN02_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCS1641603.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22282..24216
                     /gene="rnb"
                     /locus_tag="OQN02_000689"
     CDS             22282..24216
                     /gene="rnb"
                     /locus_tag="OQN02_000689"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1641604.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24294..24396)
                     /locus_tag="OQN02_000690"
                     /pseudo
     CDS             complement(24294..24396)
                     /locus_tag="OQN02_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24450..26424
                     /gene="pdeR"
                     /locus_tag="OQN02_000691"
                     /pseudo
     CDS             24450..26424
                     /gene="pdeR"
                     /locus_tag="OQN02_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26572..26745
                     /locus_tag="OQN02_000692"
     CDS             26572..26745
                     /locus_tag="OQN02_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641605.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26835..27584
                     /locus_tag="OQN02_000693"
     CDS             26835..27584
                     /locus_tag="OQN02_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1641606.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27853..28071
                     /gene="osmB"
                     /locus_tag="OQN02_000694"
     CDS             27853..28071
                     /gene="osmB"
                     /locus_tag="OQN02_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1641607.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28197..28523)
                     /gene="yciH"
                     /locus_tag="OQN02_000695"
     CDS             complement(28197..28523)
                     /gene="yciH"
                     /locus_tag="OQN02_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1641608.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28523..29260)
                     /gene="pyrF"
                     /locus_tag="OQN02_000696"
     CDS             complement(28523..29260)
                     /gene="pyrF"
                     /locus_tag="OQN02_000696"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1641609.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29453..30622)
                     /gene="lapB"
                     /locus_tag="OQN02_000697"
     CDS             complement(29453..30622)
                     /gene="lapB"
                     /locus_tag="OQN02_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1641610.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30629..30937)
                     /gene="lapA"
                     /locus_tag="OQN02_000698"
     CDS             complement(30629..30937)
                     /gene="lapA"
                     /locus_tag="OQN02_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1641611.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31086..31850)
                     /gene="pgpB"
                     /locus_tag="OQN02_000699"
     CDS             complement(31086..31850)
                     /gene="pgpB"
                     /locus_tag="OQN02_000699"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1641612.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32020..32610
                     /gene="ribA"
                     /locus_tag="OQN02_000700"
     CDS             32020..32610
                     /gene="ribA"
                     /locus_tag="OQN02_000700"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1641613.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32674..35349)
                     /gene="acnA"
                     /locus_tag="OQN02_000701"
     CDS             complement(32674..35349)
                     /gene="acnA"
                     /locus_tag="OQN02_000701"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS1641614.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35513..35608)
                     /locus_tag="OQN02_000702"
     CDS             complement(35513..35608)
                     /locus_tag="OQN02_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641615.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35722..35889)
                     /locus_tag="OQN02_000703"
     CDS             complement(35722..35889)
                     /locus_tag="OQN02_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641616.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35892..36056)
                     /locus_tag="OQN02_000704"
     CDS             complement(35892..36056)
                     /locus_tag="OQN02_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1641617.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36351..37325)
                     /gene="cysB"
                     /locus_tag="OQN02_000705"
     CDS             complement(36351..37325)
                     /gene="cysB"
                     /locus_tag="OQN02_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1641618.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37535..40132)
                     /gene="topA"
                     /locus_tag="OQN02_000706"
     CDS             complement(37535..40132)
                     /gene="topA"
                     /locus_tag="OQN02_000706"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1641619.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40512..40763
                     /locus_tag="OQN02_000707"
     CDS             40512..40763
                     /locus_tag="OQN02_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1641620.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40799..41848)
                     /gene="sohB"
                     /locus_tag="OQN02_000708"
     CDS             complement(40799..41848)
                     /gene="sohB"
                     /locus_tag="OQN02_000708"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1641621.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42068..42826
                     /locus_tag="OQN02_000709"
     CDS             42068..42826
                     /locus_tag="OQN02_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1641622.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42823..43413
                     /gene="cobO"
                     /locus_tag="OQN02_000710"
     CDS             42823..43413
                     /gene="cobO"
                     /locus_tag="OQN02_000710"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS1641623.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43453..44325)
                     /gene="rluB"
                     /locus_tag="OQN02_000711"
     CDS             complement(43453..44325)
                     /gene="rluB"
                     /locus_tag="OQN02_000711"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1641624.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44426..45046)
                     /locus_tag="OQN02_000712"
     CDS             complement(44426..45046)
                     /locus_tag="OQN02_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1641625.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45043..45924)
                     /gene="yciV"
                     /locus_tag="OQN02_000713"
     CDS             complement(45043..45924)
                     /gene="yciV"
                     /locus_tag="OQN02_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1641626.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46062..46106
                     /gene="trpL"
                     /locus_tag="OQN02_000714"
     CDS             46062..46106
                     /gene="trpL"
                     /locus_tag="OQN02_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1641627.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46083..46176
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46198..47760
                     /gene="trpE"
                     /locus_tag="OQN02_000715"
     CDS             46198..47760
                     /gene="trpE"
                     /locus_tag="OQN02_000715"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1641628.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47760..49355
                     /gene="trpD"
                     /locus_tag="OQN02_000716"
     CDS             47760..49355
                     /gene="trpD"
                     /locus_tag="OQN02_000716"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1641629.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49356..50717
                     /gene="trpCF"
                     /locus_tag="OQN02_000717"
     CDS             49356..50717
                     /gene="trpCF"
                     /locus_tag="OQN02_000717"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1641630.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50729..51922
                     /gene="trpB"
                     /locus_tag="OQN02_000718"
     CDS             50729..51922
                     /gene="trpB"
                     /locus_tag="OQN02_000718"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1641631.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51922..52728
                     /gene="trpA"
                     /locus_tag="OQN02_000719"
     CDS             51922..52728
                     /gene="trpA"
                     /locus_tag="OQN02_000719"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1641632.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53109..53288
                     /locus_tag="OQN02_000720"
     CDS             53109..53288
                     /locus_tag="OQN02_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS1641633.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53374..53874
                     /locus_tag="OQN02_000721"
     CDS             53374..53874
                     /locus_tag="OQN02_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1641634.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53920..54426
                     /locus_tag="OQN02_000722"
     CDS             53920..54426
                     /locus_tag="OQN02_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1641635.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54486..55124)
                     /gene="ompW"
                     /locus_tag="OQN02_000723"
     CDS             complement(54486..55124)
                     /gene="ompW"
                     /locus_tag="OQN02_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1641636.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55364..55453
                     /locus_tag="OQN02_000724"
                     /pseudo
     CDS             55364..55453
                     /locus_tag="OQN02_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55481..56224
                     /locus_tag="OQN02_000725"
     CDS             55481..56224
                     /locus_tag="OQN02_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1641637.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56254..56793
                     /locus_tag="OQN02_000726"
     CDS             56254..56793
                     /locus_tag="OQN02_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1641638.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56898..57296
                     /gene="yciA"
                     /locus_tag="OQN02_000727"
     CDS             56898..57296
                     /gene="yciA"
                     /locus_tag="OQN02_000727"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1641639.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57336..58064)
                     /gene="tonB"
                     /locus_tag="OQN02_000728"
     CDS             complement(57336..58064)
                     /gene="tonB"
                     /locus_tag="OQN02_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1641640.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58279..58575
                     /locus_tag="OQN02_000729"
     CDS             58279..58575
                     /locus_tag="OQN02_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1641641.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14062 a        15150 c        14646 g        14718 t
ORIGIN      
        1 actaatgcac cttcctgctg taatgcctcg accagcgcta atatttcgct ttctggaagc
       61 aagccacgca gtgggttcaa cccgatgcca tcgtgagagg cgaggaagtt aaaccaggtc
      121 gttttactgg agggcaagct cagactttgc gcccactggc acagcgcctc gacgttctgt
      181 ttttgcaccg catgtaacac cagcggcggc agcgagaact ggtacaccat atgcgcttcg
      241 tcatcaccgt tgccaaagta agcaatgttg tctttgtgcg gaacattggt ttcggtaatg
      301 atcactgtac ctggtgctac gtcatcaata atcgaccgta acagcttgat aatcagatgt
      361 gttttttcca gatggatgca gcttgttccc ggctctttcc acataaagcc aacggcatcc
      421 aggcggacat actcagcacc tttctcaagg taacagagta aaacatccac catcgccagc
      481 aacacttcag ggctacggta attcaggtca atttgatcgt cactaaaggt ggtccataaa
      541 tggcgcgtag aattatcatc catttgaaac ggcgttaata gcggtaacgc acgcggaagg
      601 gtgacggcac tgagatcggt tttcggatca accgcaataa agaaatcttc aaaacccgga
      661 agctgttgta aatagttttt aaaccattca ctttttgcag acatatggtt gcagacaaaa
      721 tcaaacatta aatgactgca ttcaccgagt tgctgaatat cctgccactc ccccgcttca
      781 ctggcgacct gatgataatc aattaccgaa aatccatcat cagatgacca tgggtaaaaa
      841 ggtaataaat gaacatgtga aaaaatgctt tgcagccatt gatggtagaa ctgattaaat
      901 atgggtagtg gttttaaatc attactgtga aattgatcgg cataggtaat taacacgaca
      961 tcactttcat cccagtgttt tttacgtcgc tgtgtaatta atcgtttcgc actctcaaga
     1021 cgcgttacaa gtttctgtaa atgagttgcg gtaaatgttc caccgtagat ttcgtccatg
     1081 taatccgtaa ttttctgttt catgttgcct ttccgtggta gcggtcccat gaggcgagag
     1141 actactccgc agaaaataaa ctgtcaattg agcacaaggg ttttagtcac gcaggaaatc
     1201 attttttacc agggaaaaag cgtatttgtg agctgacgca aataatctat agggatgtaa
     1261 ataaggtgtg acagaaaaaa aacggcgcat aagcgccgct catggtgaat tcttttaacc
     1321 tttgaccttc ggcattgcga tgtctttcag gccaccggcg ttctcaacgt gggtatatcc
     1381 catctcgcta aggagctctt ttgcctgccc ggactggcgt ccggcattgc aatacacttt
     1441 cacggtgtcg tttttatccg gaacggcggt ggcaatgcgc tctttcactt ccttcagggg
     1501 aatattgatg gccccctgaa cgtgctcttg ctgatactgc tctggaacac gaacatcgat
     1561 ccagtgttca gcggcgaaaa cgggcagtga aaacaccagt gccagagcaa gtaagccttt
     1621 ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata cgcctgagat
     1681 ttaacaattt ttgcgaaact cccctcacct tttataacgc tgtgccagtt tattagcagc
     1741 ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc ggcgagcaac
     1801 tgattttatc gcccagcccg ccacacccgc cgggccatag cgcagtgcgg taagaagtac
     1861 cagcttgcct gctaatttga aaccaagctt caccttttgc ccggcctgtt gccagcgagt
     1921 attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt atcggaagtg
     1981 acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg gtcgacttca
     2041 tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc cggcattgga
     2101 tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag accgaagaaa
     2161 atcgacagca ccaccaggat acgcaccagt tttaccggta catcaaaata gttggcaatc
     2221 ccggcgcaga cgccgcggac catgccctgc tgtggaatac gccataactt tttattgaga
     2281 ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga atagattcca
     2341 gcgcctgaat acgttcgcgc atccgttttg cttcatcaac cagttgcgct aatcgctgct
     2401 gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc cataaccaga
     2461 tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc gcgctcataa
     2521 gtactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt attattgatt
     2581 gtcttgcttc attttggctt ttaattgtgc cagttgttcg ctgattgcat catcggcttt
     2641 cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc tttctgcttc
     2701 cgcttccatc tggtcaatac gacgttcgaa agattcgaaa cgagccattg cttcatccag
     2761 tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct gatgacgtaa
     2821 catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct cgccaatctc
     2881 tttcttcatg cgtgccagcg tatcgtccac cagcgtcact tcatgttcca gggacttaat
     2941 cagatcggtc agtttctgtt tttcaattaa cgctgcacgt gccagatcct ctctctcttt
     3001 cagcagcgcc agttcggctt tttcctgcca ttcaacttca cgcgccgacg cttgttcaat
     3061 acggcgagtc agctgtttct tttctgccaa cgcacgcgcc gaagtagaac gtacttcaac
     3121 cagtgtatct tccatctcct ggatcatcag acgcaccagt ttctgtggat cttccgcttt
     3181 ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa aaatacccat
     3241 aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc tgaactgtta atacaatttg
     3301 cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaatca acagcaacat
     3361 gccaggatga attagcgaat tacactaaca agtggcgaat ttcatcatgg ccgaatacaa
     3421 agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg tttcgcatct
     3481 cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta aagagttgat
     3541 tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt cccttaactg
     3601 cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag cgggggcgtt
     3661 taccggtgcg caaaaacgtc atcctgggag atttgaacgt gccgacggcg gtacgctatt
     3721 tcttgatgaa ctcgctacgg caccgatgat ggtgcaggaa aaattattgc gtgtgattga
     3781 gtacggtgaa ctggagcgcg ttggcggtag ccagccgttg caggtgaatg tgcggttggt
     3841 atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggtacttttc gcgctgacct
     3901 gctcgaccgg ctggctttcg atgtagtgca actgccgccc ctgcgcgagc gcgaaagcga
     3961 cattatgctg atggcagaac actttgccat tcagatgtgt cgggaaatca agctgcctct
     4021 gttcccgggg tttacggagc gcgccagaga aacattgctg aattatcgtt ggccgggaaa
     4081 tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca ccagcgatta
     4141 tccgcttgat gacatcatta ttgatccctt taaacggcgt ccacctgaag aagccatcgc
     4201 cgtttcagaa aatacctcgc ttccgacgct gccgctggat ttacgcgagt ttcagatgca
     4261 gcaggaaaaa gagttgctgc aactcagttt gcaacagggg aaatataacc agaagcgcgc
     4321 ggctgaatta ctggggttaa cttatcatca gttccgcgcg ttgttgaaaa agcaccagat
     4381 ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg aatgctttat tcggcataca
     4441 taatcaatac gtcgtttgca ggcctgataa gcgcagcgca tcagacatta ttgcgttggg
     4501 ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc gcatcgaatt gcgcatccgg
     4561 gatggtcagc ggatacagga agcgaatcac gttgccgtat gcgccacagg tcagcaggag
     4621 cagcccctgc gccagcgcgc gttgctggat tttctgtgca atcgccgctg acggctcgcc
     4681 cgtttgcgga tcgttaaact ctgccgcaat catcgacccc aggccgcgta ccgcagcaat
     4741 ggccggaacg ctttctttgg catcaatcaa cgtgtttttg agacgctggc ccagttgatt
     4801 cgcgcgttcg cagagtgatt ctttgtcgat aatattgagc accgcgtgcg cggcagccac
     4861 cgccagcggg ttcccggcgt aggtgccacc caacccgccc ggcgcgggcg cgtccataat
     4921 attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc gcgaggcttt tcgccatcgt
     4981 cattaagtcc ggcttatcgg cgtaatgatc catggcaaac agtttaccgg tacgcgcaaa
     5041 gccgctttgc acttcatcgg caatcatcac aataccgtgc tcgtcgcaca ggcggcgaat
     5101 ggcggcaacc agctcttttg gcgcaacgtt gaaaccgcac tcgccctgca ccggttcgaa
     5161 aataatggcc gcgacctgct tcgcttcgat gtctgattta aacaagcgtt cgatggcgtc
     5221 gagggagtcc tgtgttgaaa tgccgtgtaa atctgacgga taaggtacgt gatacaccga
     5281 accagggaac gggccgaagc cgattttgta cggcgcaact tttccggtca gcgccatggt
     5341 catatatgta cgaccgtgga agccaccgct aaacgcaatc acgccaggtc gtccggtatg
     5401 ggcgcgagca attttcaccg cgttttccac cgcttccgcg ccggtagtga agaatgcggt
     5461 tttagcctgc ccgctcactg gggcaagggt gttaattttc tccgccagcg tgacgtagct
     5521 ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt tgcaattgct gctccaccgc
     5581 cgcgaccaga tcagggtggc gatgcccggt attcagcacc gcaatgcctg cggcgaaatc
     5641 gatgtattcg ttgccctcta cgtccttcag cgtagcgttt tctgccgact gggcgaagaa
     5701 gttacacatc acgccaaccc cgcgtggggt agcagaaaga cgacgctgat ggaattcatt
     5761 gttgctcatg tttcacacct tttgactctt cactgttaat cggaaattaa acgcccagac
     5821 gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa tggcacgcgc agcgtgcgcc
     5881 cgccggggaa cgggtaatgc ggcagattgg cgaaggcatc gaaacgctcg gcgtcaccgc
     5941 gcagcagttc agcaatcaaa cgcccggcaa gatgggtaca ggtgacgccg tggccgctgt
     6001 aaccctgcat gtaatagatg ttggtatcaa ggcggccgaa ctgcggcatg tgcgacaagg
     6061 tcagcaggaa gttgcccgtc cagcggtaat caattttcac gcccttcaac tgagggaagg
     6121 tttttagtag tttcggtacc acaagacgct caacgtcatc cgggttgcgt gcaccataca
     6181 ctacaccccc gccgtacagc aggcggttgt cggcggtaag acggtagtaa tcaagcagat
     6241 agttacagtc ttccacacag tagtttttcg ggatcagcga acgggctaaa tcttccgaca
     6301 gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct gcgtttcgct agttccggct
     6361 ctactttatc gcccagatac gcattcccgg cgacaatcac atacttcgcc gtcacctgac
     6421 cattggcagt ccgaaccacg gctggcgtgg tgtgctggat ctgcgtcacg gcggaaagct
     6481 catacacgcg cccgccgttc aggcggatgg catcggcttc accaatggcg aggtttagcg
     6541 ggtgaatatg cccaccgctg tgatccagca gcgcaccagt gtagcgatcg ctggcgacct
     6601 cgcggcgaat agcgttgacg tccagcagct ccagctgttt attgccgtag cgttcccagt
     6661 tctctttctg ctcttcgagt gtggcgagtt gtttatcatt catcgccaca aacagaccgc
     6721 cggggcggta gtcgcagtca atctgataac gtttgatacg ttcgcggatg atctcgccac
     6781 cctcgaacat catgctgccg agcatacggg cggtgtccat gccgtagctt ttttcgatca
     6841 cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc attgcgcccg cttgcgccaa
     6901 agccgatgcg tgaggcttcg agaaccacta cgtcaaagcc cgcttccgcc agatgcagcg
     6961 cggaggagag cccggtatag ccgccgccaa ccacgcaaac gtcgcaggtg attgactcat
     7021 tcagtgtgtc gaatggtgca tatttattcg cactggcggc gtagtaactg ctggtatgtt
     7081 cggtcatttc aggcctccag gcttatccag atggttttca gttcagtgaa tttttcaagg
     7141 gcatgcaggg atttgtcgcg accgttgccg ctctgcttat agccgccaaa cggcacggtc
     7201 atatcgccgt cgttgtagtt attgacgaag acggaaccgg ctttcaggcg tcggctcatg
     7261 cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc caaggccgta ctggctgtcg
     7321 ttggcaagct gcagcgcctg atcttctgat gtgaaacggg tgaccaccag caccggaccg
     7381 aaaatctctt cgcggcttaa ggacgcattc gggtccacat ccacgaagat ggtcgggccg
     7441 atggccgctg ccagcccggc gttgcgacca tccagcagca gttgcccctt gccttcgcct
     7501 tcacgaataa aggtgtgtac cgagtcagcg tgagcgctgt cgattaaggt gcccatggtg
     7561 gttgcgggat caagtggatg gcccggctgc cagttttgcg cctgctgttt taacagggct
     7621 aagaattcat cggcgatgct ctcttccagt aacaaacgcg ttccggcgat gcacacctgg
     7681 ccctggttgt agaagatgcc tgcggcggtg gcgcttgccg ctttttgcag atccggacaa
     7741 tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca gccagacacg tttcatgttg
     7801 ctatcacccg cgtctttcag cagttgttta cccgtacgcg tggagccggt aaaggcgatg
     7861 gcatcgatat cgttatgacg cgaaagcgcc tgccccgctt catgaccaaa acccgtcacc
     7921 acgttcaaca caccatccgg caagcctgct tctttcgcca gccccgcgag acgaatcgca
     7981 ctgagcggtg atttttcaga cggttttaga atcacgctgt ttcccgccgc cagcgccggg
     8041 ccgagtttcc agcaagtcag caacagcggg aagttccacg gcacgatggc ggcaatcacg
     8101 ccgaccggtt cacgcacgat catcgccagc tcatggctac tggtggtcgc cacttcgcca
     8161 tacactttgt cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca
     8221 tcacgcagac tgtgacgaat cggtttgccg gtgtcgagag tttccagcag tgccagctct
     8281 tcggcgtggg cttccattaa atcggcgagt ttattcagta ccgctttacg tttagccgga
     8341 gaagagagtg accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac cgcacggtcg
     8401 atatcgacgc tcttgccgcg ggcaattttc gccagcggtg cctgggtgac cggatcaacg
     8461 gtttcaaagg tttcattttc cgccgcagca gtatattcac cgttaataaa taagcggttt
     8521 tcaatggcga gacttaacgc tttatcctgc cagtaagcca gatgatgaaa attcattatg
     8581 actcctgttt cacgtctatc agatatatgc agtttgtgaa cgtcagggcg cagagatccc
     8641 cgggctgcat gcccgtttat ttctggcgac attctttatg gattgccggg cgcattgcgc
     8701 ttacccggct tacagaacaa aaagattaaa acgtggtggg cgtatgggcg ctgataattc
     8761 ggcaaatacc tgccgacgta ttactgaaac tgtgcgggat gccggtatta atggcataac
     8821 tttgccccgc gacgaggtgg taatcctgac cattaatcgt cagaacaatt tcaccttcca
     8881 gtactgtgcc tatttcctca ccctgatgct taattctttc cccagtggtt gtgcccggct
     8941 ggtacgtttc aaagatcatc gccagcgtgc gattcgggtt accgttatga accagcttca
     9001 ttgacacacc ctgactgccc atctcaatta agtcgtcctg attaatgacg acctgcggct
     9061 catcaggttt ttccggctcg gaaaagaatt ccgagagtga caaaccatac accttcagca
     9121 gcttttgcag cgtactgatg gcagggctga ctttatcttg ttctatcgta ctgatagcac
     9181 tgtgagtcag cccggagagt tcggcggcac gacgttgtga aagcccctgc tgctggcgga
     9241 tttccgacaa gcgttttcct ggcgccagtc cctcatcact catatttgca tttccttaaa
     9301 ctgtagtggt cagagtcgct gtttttcagc gatatggtgc tgccaagcgg tgataaagcc
     9361 ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc cattccgggt gccactgtac
     9421 gcccagcgca aaaggatgat tgatgacgct aaccgcctca actaagccat ccggcgagcg
     9481 agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct tgtccatgta gagagtttac
     9541 ccaaaagttg ctacattcag gtaacaacgc agacagtaat cccccctctt caacctgaac
     9601 ttcatgcgac ggtgcatatt gctgttccac cggcagttcg ggatcttccc gatgttccag
     9661 caattcaggc tgttcgcaca gcttgcgatg caacgaacca ccggttgcca ccaccagttc
     9721 ttgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt tcgagtgcgg cattaattat
     9781 cgccatgctc agaagatcac gcccgggatc ggcgtcaggc tcatcgccgt tttcaccata
     9841 taggtgcggc tgcacattgc tgggactact aggaagataa atgccatcga gtttcggcaa
     9901 aagttgttca agtaacgacg gttccgccag cgcatgtggt agcgcaattg gcaagccgcc
     9961 tgcgtggatg atggcattca ggtacttttc ttgcagagtc tgggtcgcat gacccttaag
    10021 cctgttcctg cacattacga caccgataac cggattgttc attatatttt ccatgctcaa
    10081 tctcacaaag tggactaaat tatcgccatt actgcgcttt aaatgacctt tatgttcaat
    10141 attttttcaa tctagcagtg gattttcatt tttgcaaact caatttaaca tttgacaaac
    10201 atttggtttg catacagatt cgaatgatgg tcattatatt ttacgctttg ataacgagcg
    10261 gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc gttgaagtag agaactttgt
    10321 tcagcagtca gaagagaggc ggggtagcac ctttacgcag gaagtgaaac gctacctgga
    10381 gcgctacccg aatacgcaat atgttgatgt tttacttact gatttgaatg gttgcttccg
    10441 tggaaaacgt atccctgttt caagcctgaa gaagctcgag aaagggtgtt atttcccggc
    10501 ctcggtattt gcaatggata ttctgggcaa cgtagtagaa gaagcaggtc tgggtcagga
    10561 aatgggcgag ccggatcgta cctgtgttcc tgttctcggt tccttaactc cttctgccgc
    10621 cgatccagag tttatcggtc agatgctcct gaccatggtc gatgaagatg gcgctccctt
    10681 tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag cagctgcgcc agcgcggatt
    10741 gttcccggtt gtagcggtag agctggagtt ctatttactg gatcgccagc gcgacgctga
    10801 aggggatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac aaagccaggt
    10861 ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg atgaactggc
    10921 gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg gtcagtttga
    10981 aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc tggcactaaa
    11041 acgcctagtg cgtctgatgg cagaaaagca taagatgcac gccactttta tggcgaagcc
    11101 gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa ataatcgtgg
    11161 cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa agatgctcgc
    11221 cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg tgaactcgta
    11281 tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc ataacaaccg
    11341 caccgtcgcc ctgcgtattc cgtgcggtga ccgtcataat caccgcgtgg aatatcacgt
    11401 ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg gtattttgca
    11461 tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc tggaacagga
    11521 aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg agaatgatca
    11581 cctgcgccgc tatttaggcg aacggttttg ccatgtgtat cacgcctgta aaaatgatga
    11641 gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt tgaaaaacgc
    11701 gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg acatttgctg
    11761 gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg ggcttccgtt
    11821 aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc ccgcatggcg
    11881 agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct ttgcttccct
    11941 tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc aggggaggca
    12001 gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa aacccgtctg
    12061 cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct cacgccgatg
    12121 accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt tccggcgtcc
    12181 tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa actggttcgc
    12241 cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa cccgcacgtc
    12301 ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat gatcaacgtt
    12361 ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg ggtgtgggtg
    12421 gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct ggtcgctaac
    12481 ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat cttcctggtg
    12541 gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca gccgtttatc
    12601 agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg tttctcgttc
    12661 ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc acgcgtgatc
    12721 ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc ggcgtcgttc
    12781 tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc cgcactgcct
    12841 gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg caccacgttt
    12901 gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct gctgtatgtg
    12961 atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc aaaatggcgg
    13021 actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct gttctttgat
    13081 ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac cttcgtgaat
    13141 ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg gaaggatcac
    13201 ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct gtgggttaac
    13261 ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg cgcatatttg
    13321 tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag aacgccggtg
    13381 agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa cgcgccgcgc
    13441 cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc ccttatgctt
    13501 agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat attttgatat
    13561 tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac gcacaccgct
    13621 ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga tcagcgaaga
    13681 agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg atgagatcgc
    13741 agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat cggtcagtat
    13801 cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca ctgcagcgtg
    13861 ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt ctgtgagtta
    13921 tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac ctgttcaacg
    13981 accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca aatgtcatta
    14041 aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc aggtattatc
    14101 gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc ctgaatcccc
    14161 cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc aagtcaacac
    14221 ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg cccagtttta
    14281 tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgcctgaac ttgccgaaag
    14341 ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg atgttccgtt
    14401 tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg tggtgtttac
    14461 cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca gcaacttccc
    14521 ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac tggataatca
    14581 taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc tcgcaaccca
    14641 ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag atcgccagga
    14701 gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat accgcgccgg
    14761 gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt taatgccgca
    14821 ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc tgaccgggga
    14881 atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg acgacccgcg
    14941 cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta acaccgccaa
    15001 accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta ataaccagcg
    15061 cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt taccgcgcgc
    15121 ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga aatcgcgcga
    15181 acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc ccacacgttc
    15241 gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg atatggcgca
    15301 ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg cgcggttgat
    15361 ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg acccggacag
    15421 tttcttccgt ccgttactga gctgcgcggc aattcattca cagaccaacc tcgcccactg
    15481 gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc agctggcagc
    15541 gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac ctattctgcc
    15601 gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc tggtacttag
    15661 cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg aggtgaaaaa
    15721 accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt gttcctgctg
    15781 accttcgttg gcttcagctt gagctatttc actccacacg cgccgttaca aggcgcgtca
    15841 ctgtggaatg cctgggtgtt ctggtttaat ggcctgatcc actgggattt tggtgtttcc
    15901 agcattaacg gtcaaccaat tgccgaacag ttaaaagagc tcttcccggc gacgatagaa
    15961 ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg gatgattgcc
    16021 gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt actgggcttt
    16081 tcaatcccgg tgttctggct ggcgcttctg ttgacactgt tttgttcact cacgctaggt
    16141 tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat taccggtttt
    16201 gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat catgagcgca
    16261 atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac tgaagtgatc
    16321 cgcctgatgc gtatcagcac tattgaagtg tatgaccaga actatgtaaa agcggcggcg
    16381 acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa cgcgttaccg
    16441 ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc gatgatcacc
    16501 gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat ccgccagcag
    16561 gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat tattgttaac
    16621 gtgatttctg atattttggg tgccatggct aaccctctga aacataagga atggtatgcc
    16681 ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg cctggcgcaa
    16741 attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcgggac tggctgtact
    16801 gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc tcggttatca
    16861 attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg ggactgacga
    16921 cctcggacgt gatgttttaa gccgtttact gagcggagct gcgccaaccg tgggtggcgc
    16981 atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct tcgccggggc
    17041 gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac tggcgatccc
    17101 ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt cccacgctat
    17161 gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca gcatggtgca
    17221 tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat caacgctgaa
    17281 tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg agatcacccg
    17341 cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc tcggcgcaca
    17401 actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga tttatgtcgc
    17461 cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt tggttaacct
    17521 ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc attactcgat
    17581 attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc cgtcgaccgg
    17641 gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc cggttccggc
    17701 aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg tgttactgct
    17761 gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga acggcgcaaa
    17821 ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct tgacccttca
    17881 gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa aggccgttgg
    17941 tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg cgtggggatt
    18001 aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg tgaatgtcag
    18061 aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc tgacgaaccg
    18121 acaaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac gcgtctcaac
    18181 caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct tagccaatgg
    18241 gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc gagtaaggag
    18301 ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat accagacttc
    18361 ggcagcgcga tgccgcataa aagtcgcctc aatactctac ccggcgctat cccattgctg
    18421 gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca acgagaatgc
    18481 attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca tttcccgctg
    18541 aacatggaga aaaagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt taagtaaaac
    18601 gttccgctac cggaccggtt ggtttcgtcg tcagaccgta gaagcggtaa aacccttgag
    18661 ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt cgggtaaatc
    18721 cacactggca aaaatgctgg cgggaatgat agaacctacc agcggcgagt tattgattga
    18781 cgatcatccg ctgcattttg gcgattattc cttccgtagt cagcgtattc gcatgatttt
    18841 tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcacaaattc tggattttcc
    18901 actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta ttgaaacgat
    18961 gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt tggcacccgg
    19021 acaaaaacag cgtctgggtc tggcgcgcgc gctgatattg cgcccaaaag tcattattgc
    19081 cgatgaagcc ctcgcttcac tggatatgtc aatgcgttcg cagttgatta atctgatgct
    19141 ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata tcggaatgat
    19201 gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg agcgaggcag
    19261 caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga ttgccgggca
    19321 ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt ctgaatcctc
    19381 cttccagatt attcacctca ttctgacaag agattaaatc ttgtcagaat gaggtgatgg
    19441 ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag gaacgaaagc
    19501 tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt caacatccgg
    19561 tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt gagctggatg
    19621 gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg cttgagtcgc
    19681 agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag gaggcggtgc
    19741 tggagattat tctgcaggaa ctgaaccgcc ggtcaccctc accctgaaag ggcgaggggg
    19801 ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga gaatattcgg
    19861 caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat gagcctgttt
    19921 gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg gagagggtta
    19981 gggtaagggg aaaagcgccc cccctgccgc agcctgctcc ggtcggacct ggcaactata
    20041 gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt ttactaaaac
    20101 gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa agctatgggt
    20161 tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc catcgcctac
    20221 ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta ccagaacgac
    20281 aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat cgttctgcag
    20341 tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg gaaagtttgg
    20401 ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca gctggatggt
    20461 gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat cagctcctac
    20521 agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc tgccctgctg
    20581 accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat gggtctggca
    20641 aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg
    20701 cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcctccgg tatcaaagac
    20761 ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac agttactatt
    20821 gaagatgtgg gtaactctgc ggcattcctg tgctccgatc tctctgccgg tatctccggt
    20881 gaagtagtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact cgaactgaaa
    20941 taatcgttct gttggtaaag atgggcggcg ttctgccgcc cgttatctct gttatacctt
    21001 tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg
    21061 atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc aaaagccact
    21121 ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg gtcccggaag
    21181 ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagag acgctggccc
    21241 ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc
    21301 ttaccgtgac ccgttcacaa accttcaccg cttatgaacg cttgtcgacg gccctgacgg
    21361 ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga cttacgccgc
    21421 tccccggacc tgattcctcc agggaaagta atcatcggtt ggcacaaatc acgcaatatg
    21481 cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag catctgaatg
    21541 acgtcggtct tactgtctgg gactgtgtga tcattaacca aatcattggt tttattggct
    21601 ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac
    21661 ccgggctggc gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct
    21721 ggcgaagcag ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt tcaactgcag
    21781 aactttgcca actggccgaa acacactctc tccaccctat ttcactttcc cttctcgaaa
    21841 agttgttaaa cagcacacgg gtcaatgcac agccggataa tcagcttgcg gcgttgttat
    21901 gcgcgcgtat aaatggcagt cctgcttgtt tttccacctg tatggattca tcaaatgaat
    21961 ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc
    22021 gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc
    22081 gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca acgcagatta
    22141 ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg
    22201 gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa
    22261 tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag
    22321 caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt
    22381 ggcttcctgg aagtcgacgc acaaaaaagt tatttcattc cgccgccgca gatgaaaaaa
    22441 gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg tgaatccgca
    22501 gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt tcagggcaaa
    22561 aatgaccgtc tgaccatcgt tcctgatcat ccacttttaa aagacgccat tccttgccgc
    22621 gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc
    22681 cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt
    22741 ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct ggaaaaagaa
    22801 gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc
    22861 tcgctggatt ttgtcaccat cgacagtgcc agcactgaag atatggatga cgcccttttc
    22921 gctaaggcgc tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg
    22981 tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat
    23041 ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg
    23101 cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc
    23161 actgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat
    23221 gaccaggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt
    23281 gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac
    23341 cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa
    23401 gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg
    23461 attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat
    23521 aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg
    23581 cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg caaactgcgt
    23641 cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca
    23701 tttgctgaaa ttagctctga acccggtcct cactttggcc tcggtctgga agcatacgcc
    23761 acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg
    23821 gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgaa
    23881 cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata cgcacgcttc
    23941 ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt
    24001 ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc
    24061 ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa
    24121 ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga agtccgcatg
    24181 gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctca
    24241 tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat tttttaactg
    24301 actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata tataaaaaac
    24361 aatgcgttta gccgccgtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg
    24421 actccgctgt taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt
    24481 acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgctttgc
    24541 gcttttctac caccaaaacc acagaacctg atcgtacatt gcagttatct gccgaacagg
    24601 ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg
    24661 atgaaagcga tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg
    24721 gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg tgatttatca cacgggcttt
    24781 cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg
    24841 ggaatatcca acgcttcaat cggttatgtg aagattacac agggttgaaa gaacacgacg
    24901 tcattgggta aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc
    24961 gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa
    25021 cacgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca
    25081 aaaacgagat ttttttaatc tgttccggta ccgacattac cgaagagcgc cgcactcagg
    25141 agcgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa
    25201 tgcaggagtt aatcgatcac gctattaatc aggcagataa caataaagtt ggggttgtgt
    25261 atcttgattt ggataatttt aaaaaggtca acgacgccta tgggcatttg tttggtgacc
    25321 agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg
    25381 cgcgtccagg tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg
    25441 aagcaatggc atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg
    25501 aagtttatac cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg
    25561 cgactattat tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac
    25621 aattttgcgt ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata
    25681 ccaacttgcg taaagcactg gaaaacgatc agttggttat cgaaaatcac ctagcgtggc
    25741 gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg gttgattcca
    25801 ccgttggact tcatttccta cgccgaagag tcagggctaa ttgtgccttt aggccgttgg
    25861 gtgattctcg atgtcgtacg ccaggtggca aagtggcggg ataaaggcat aaacctgcga
    25921 gtggcggtaa atatttctgc acgtcagctc gccgatcaaa ccattttcac cgccctgaaa
    25981 caggttctcc aggaactcaa ttttgaatac tgccctatag atgttgaact gacagagagt
    26041 tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag ccaactaggt
    26101 gcgcaagtgc atctggatga ttttggtacc ggctactctt cactttcgca actggcgcgc
    26161 tttccgatcg atgccatcaa acttgaccag gtttttgttc gagatattca caaacaacct
    26221 gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg
    26281 atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat
    26341 gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga acgctggtat
    26401 aaacgctatc tgaagcgcgc ataagtagat gaatatatat acttagcgct atatcccgtc
    26461 attatatatc gtattcctgg atgataatta gccgttgctg ttaaacactt ctcttttagc
    26521 ggcaattttt gcattattca ttttccccct tttgctataa ggatgtcctt gatgtctgaa
    26581 tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct tgtggcaggc
    26641 gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt gcgccaggtt
    26701 gctgaatcta cgccagacat cccgaagaca ccgtgggaga tttaacaatc gtgccttcag
    26761 gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat tataacgtta
    26821 taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc attgaccagg
    26881 gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt accattcgcc
    26941 aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc tttgcagttt
    27001 cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac
    27061 tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac catctttatc gaaaatggca
    27121 gcagcaatgc cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc actatcatca
    27181 cggtcagcag ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc attttgctcg
    27241 gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc tttgacacgc cagtgcatcc
    27301 agcaggtaca tttcagcaaa gcatttattg gtattgatgg ctggcaacct gaaactggat
    27361 tcaccggtcg cgatatgatg cgcaccgatg tggtcaatgc cgtgctggaa aaagagtgcg
    27421 aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac tccatcggtc
    27481 ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc gatctgatgc
    27541 atcttgagca cagtaaactc accgttcacg tcgttgacat ttaaccattt cccttctcta
    27601 cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt gataattaag
    27661 actatttacc tgttattaac actctcaaga tataaaatta ttatcagcga tataacagga
    27721 agtaattatc acctgcgtga tataaccctg cgcgcgagca gatttcacgg aataatttca
    27781 ccaggcttat tcttagctat tatagttata gagagcttac ttccgtgaat cataaattca
    27841 ggagagagta ttatgtttgt aacgagcaaa aaaatgaccg cggctgttct ggcaattact
    27901 ttggcaatgt ctctgagtgc ctgttctaac tggtctaaac gggaccgcaa caccgcaatc
    27961 ggcgcgggtg caggggcatt aggcggtgca gtactgaccg atggcagtac gttgggtaca
    28021 ttaggtggtg cagctgtcgg tggtgttatt ggtcaccagg tcggtaaata atcgtaaata
    28081 aatgtcgatt atcgactcgc aattataata cgcttgattc tgttcagtac gaaataactg
    28141 aataatacac acagtaaaat attcgaaagc cacggatata tccgtggctt tttatgttaa
    28201 ccgcctgcga gttttacctt catccctttc gcttcaagta gtgattttaa taaatcacgc
    28261 ttatcgccct ggatttcaat aactccatct ttaactgctc cgccgcagcc gcattttttc
    28321 ttcagttccg ctgcaagttt tgtcagttcg gcatcatcga gatcgatacc ggtaatcagg
    28381 caaacgccct tacctttacg tccactggtc tgacgctgaa tacgcaccgc tccatcgcct
    28441 ttagggcgta ctggggccgc tttgggctca tcaatacgcc cggtttccgt tgagtagacc
    28501 agacggctgt tggaatcact catcatgcac tccgctgtaa agaggcgttg atcgctttca
    28561 gtgtctgcgc tggatctacc gattgcgtta ccgggcgacc aatcaccata taatcaacac
    28621 cagccgccaa cgcctgttct ggcgtcataa tgcggcgctg gtcaccagct tcactcccct
    28681 gcggacgaat gcccggtgta accagtttga actcctgacc gaatacctgt ttaaagcgca
    28741 cagcttcctg agcagaacac accacaccat caaggtcaca tttttgcgtc agtgctgcca
    28801 gacgttctgc ataatctgca ggtgacagtg tcacgccaag atcggccagg tcgctggctt
    28861 ccatgctggt caacactgtc acagcaatca aaagcggtgc atctttgcca aacggaacca
    28921 gtgcctcacg cgctgcggtc atcatacgcg ccccaccgga ggcatgaaca ttcaccatcc
    28981 acacgcctaa gtcagctgca gcagcgacag cgtgcgctgc agtgttgggg atatcgtgga
    29041 atttcaggtc aagaaagata tcaaaaccac gctgttgaag ttcgcgcaca aactgtggcc
    29101 caaacaatgt aaacatctct ttaccgacct tcagacgaca atcgcgtggg tcgatcttgt
    29161 cgacaaagga cagcgcgtca tcacaattat gataatcaag ggcaacaacc acaggagaat
    29221 tcgtaacggc gcgggaagaa gatgaagcag ttaacgtcat gaccagacct tcttgatgat
    29281 gggcaccggg agtggcgcga aacaggtaaa cggcgagcat tctacccgtg aaaataaaaa
    29341 attaacagtt gcgatttttc ccctagcagg ctttttctgc tccgtcgttg ccggagcaca
    29401 atgggacatc aataactagc atgttgtaac taaagtcgga tttttttaaa aattacaggc
    29461 catcaagacc gcgaatcggt ttaatagttg accaggcacg acaagacgga caatgccagt
    29521 agagggtgta tgcagtaaaa ccacatttct ggcagctata acgaggctta ctacgcacct
    29581 tctcgccaac catgtcacgc agcaccatca gactctcttt ggcgcgccct tcttccgctt
    29641 catttaagtg gtaatccatt agcttatgga atacacgcat ggtcggatga cgctgaagct
    29701 ggcgtgtaat atagacctgt gcagcctcac taccgtcgcg cgcttcgatg atatcagcaa
    29761 gcatcaattc agcatcggca ccggtgttct cttccaccgc gcgttgcagg aattctgccc
    29821 attcggcagt tttacccaac tgctgatagc aggtttgcaa catttccagc gtttcgctga
    29881 ccagttctct gtcctgggat atgacgcgtt gcaaactttc gacggctttg gcgtattctc
    29941 ctttcgccat aaacacgcgt cccatcatga tggatacgcg ggcgctgttt ttatctgccg
    30001 ccgccccttt ttttagcaag gtcatggcac gatcgagatc gtcgctggcc atatgctgca
    30061 gggctaactc acagtagaaa tgggcaattt cgacgcgctg tttatcttta cccagcttca
    30121 ccaggcgttc ggcaacatca attgctttct gccactcact ggtagcctgg tagatttgta
    30181 gcaactgttg cagcgcgcca atgcggaagt cagtttcatc ggtcagctga ttgaacatgt
    30241 cttccgcgcg gtcgtataac ccggcggcca tgtaatcacg ccccagttgt tgaatcgcca
    30301 acagacgctg ttcataggtc agcgaggcgc tttccattag ggtctgatgg atgcgaatag
    30361 cgcgatcaac ttcgccacgc gaacggaaca ggtttccgag cgtaaggtgg gcttcaacgg
    30421 tgcctgtatc ctctttaagc atatcgagaa acaggtctac cgctttatcc tgttgattac
    30481 taagcaggaa gttaaccccc gctacgtaat cacgcgacaa gcggttggct tcatcttgct
    30541 tgttttgttg cgcacttctg cggcccatat accagccata ggcagcggct acaggcaaaa
    30601 gcagaaacaa caactccagc atagaaagtt attccttcgc cgctgacgag tgcggcacta
    30661 cagccacgtc agtcgcgggt gaaagctggt tttccagtcg ctttatttta cgttcagcgc
    30721 gcgccaggga aacacgaact cgcagccaga acaggccaca aatcaaccaa ccgatagcaa
    30781 accccgcagc aaacaatacc gccagcaatg tggaaatacg gtactcccct tgcgctaaca
    30841 gataattaaa cgtcacctgt tgatcgttct gcgcacccaa cgtgaccgaa atcacgaaga
    30901 tcgctaacac cagtaagaaa atgagtaaat atttcacatt acttcccgtt atgtggttag
    30961 agcgaataaa ttatggtcaa cttaccgcaa tagcccttta aagtaccatt ttcgcgccgt
    31021 ctgcgaaatg gcatccacgc gcattgttga tgatttaagg cctaaattcg gaaaatcaac
    31081 cagcgttaac tttcttgttc tcgttgtgca atttcgcgat tttcttccgc aggtggtgtt
    31141 aatggcccac aaattcgttg cgcaagccac gttgccaccg ccaccagcgc ccacgaaatc
    31201 aacgtagcta ccaccagatc gcgtggccaa tgcatcccga gcagcaggcg gcttcccatg
    31261 actcccgttg cccagaccaa caagatagca atggttaacg ttcgccgacg cggccacagc
    31321 aaaccaacgg ccagcagtgc ccaactggca gcaaacatcg tgtgaccgga aggaaaggca
    31381 aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg gaatattttt ctcttcagcc
    31441 aactgttctt ttactagatt tccgcgttct gctcgcttta aagtgtagaa ctcatcaacc
    31501 ggaatatgat gtgttttttc caaccagata acaaaaggtc gtggttcctg tactttgtct
    31561 ttgatccagg atttaacgcc ttgtccaaca aggattgccg ccgccagaat ggcaaataat
    31621 acaaaggcag ccttaatgcg aaaacgcaga caccagagaa accagccgaa caaaatcaaa
    31681 tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa cccaaaaagc cgcttttagt
    31741 agccaacttt gttctccagg ttgccaacgc cagccagaaa tccatacggc tactggcatg
    31801 acaagcaata gtgcagctcc cactgcggta cgtcgggcaa tcgaacgcat ggcctctcct
    31861 ttttgataag tcccacaatc ataactgaaa atgccagttc caagaaaaat tgacagattt
    31921 gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga accccttctc gttatggcaa
    31981 aataagccaa tacagaacca gcattatctg gagaatttca tgcagcttaa acgtgtggca
    32041 gaagccaaac tgccaacccc atggggcgat ttcctgatgg tgggatttga agaactggca
    32101 accggacacg atcatgtcgc gctagtctat ggcgatattt ccgggcatac cccggtactt
    32161 gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt tcagcttgcg ctgcgattgt
    32221 ggcttccagc tcgaagcggc attgacgcaa attgccgagg aaggccgtgg tattttgctg
    32281 tatcaccgtc aggaaggtcg taacattggt ctgctgaata aaatccgcgc ttacgcactg
    32341 caggatcaag gttacgatac cgtagaggct aaccaccagt taggcttcgc cgctgatgag
    32401 cgcgacttca ctctttgcgc tgatatgttc aaactccttg gcgtcaatga agtccgcttg
    32461 ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag cagggattaa tattgttgaa
    32521 cgcgtaccat tgattgtagg tcgtaacccc aataacgaac attatctcga taccaaagcc
    32581 gagaaaatgg gccatttgct gaacaaataa ccctcttgca tcgtgtaatt catttgcttg
    32641 ccggaagcaa aataaccggc aagcaaatac ttgttacttc aacatattac gaatgacata
    32701 atgcaaaatg ccgtcgttct ggtagtaggt caactccgtc gcggtgtcga tacgacaacg
    32761 gcagggtacg acttcctggc taccatccgc gcgcgtaagc gtcaccggaa ccgtcgcgcc
    32821 aggttgtaga ttttgcagat cgccaatatc aatcttctct tccccggtta gccctaacgt
    32881 tttacgcgtc acgccttgcg gaaattccag cggcaggatg cccatgccaa ttaaattcga
    32941 acggtgaatt cgttcaaacg attcggcaat caccacacga attccaagca gacgaggtcc
    33001 ttttgccgcc cagtcacggc ttgagcctga tccatactct ttcccggcaa tcaccgccag
    33061 cggcgtttgc tcctgcttat agcgcatcgc agcatcataa atagagacta cgtcgctgtc
    33121 aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc atttcattac ggatgcgaat
    33181 attggcgaag gtgccgcgca tcatcacttc atggttacca cgccgcgaac cgtaggagtt
    33241 aaagtctttt cgctcaacac cccgaccttg tagatatcga cccgctgggc tgtcgggctt
    33301 aatactgccc gccggagaga tatggtcagt ggtgactgaa tcccccagca ttgcgaggat
    33361 ccgcgcaccg tgaatatctt ccactggtgc tggtgttgcc tgcatttcat caaagaaagg
    33421 cgataagcga atataggttg agtcctcctg ccaaccgtag gtatcggatc gtgtgacgtt
    33481 aatttccttc cactctgctg tgccttcaaa aacttctgcg tactctttgc ggaacatttc
    33541 tgtggagact tgttctaccg cacgggcaat ttcttgtgcc gatggccaga tatctttcag
    33601 ataaaccggc tcgcctttgc gatcatggcc gataggctca gaagccaggt tgatattcat
    33661 atttcccgcc agcgcatagg caaccaccag cggtggcgag gccagccagt tagttttaac
    33721 cagcggatgg atacggcctt caaagttacg gttgccggac agcaccgcac cgacggttaa
    33781 atcgcctttt ttgattgccg tttcgatagg atcgggcagc ggcccagagt taccaataca
    33841 ggtggtacaa ccgtatccca caaggttaaa ccccagttcg tcgagatacg gtgtcagttt
    33901 cgcttttgcc agataatcag aaacgacttt cgaacccggt gccaacgacg ctttgaccca
    33961 cggttgccgc ttgaggccaa gagttacggc ttttttcgcc agcaagcctg cggccatcag
    34021 cacacttggg ttagaggtgt tggtgcacga ggttatcgca gcaatgacca cagcgccatc
    34081 aggtaactga tactgatgtc cgttcataac ataatcgact ggcaggcgat ctttatgcgt
    34141 ggcattcact tccagttcgt tactggcggc aaatgctttt ggtacatcgg gcagtgcaac
    34201 gcgatcctgt gggcgttttg gccctgccag gctcgcttca acgtcattca tatccagttc
    34261 taacatactg gtaaaaatcg gttcatcgcc cgggttacgc cacatgccct gcgctttggc
    34321 atatttttcg accaactcga cctgatcttc gctgcgcccg cttaaacgca tgtaatcgag
    34381 ggttactgca tcgattggga agaagccaca ggtggcacca tattctggcg acatattggc
    34441 aatggtggcg cgatccgcca acggtagtga atccagacca tcaccataaa attcgacgaa
    34501 tttccccacc acgccatgtt tgcgcagcat ttgggtaaca gtgagaacca ggtctgtggc
    34561 ggtaatacct tcacgtaatt ttccggtaag tttaaagccc actacgtccg ggataagcat
    34621 ggaaaccggc tggcctaaca tggcggcttc tgcttcgatc ccacccacgc cccaccccag
    34681 cacgccaagg ccgttgatca tagtggtgtg cgagtcagta ccaacgagtg tatccggata
    34741 agcaatccat tcaccgtcct gcaattcgct ccacactgct ttgccgagat attcgaggtt
    34801 aacctgatgg caaatgcctg tgcctggcgg cacgacgcta aaccgactga acgcttgctt
    34861 tccccatttc aggaacacat aacgttcgtg gttgcgctcc atttccaggc gtacgttttc
    34921 ttcaaatgcc tcatcatcac caaaacgatc gacggtcacc gagtggtcaa tgaccaggtc
    34981 gaccggtgag agcgggttaa cctttgcagt atcgccaccg aggcgtttaa ccgcttcgcg
    35041 cattgccgcc agatcaacaa cggcaggtac gccggtaaag tcctgcatca gcacccttgc
    35101 cgggcggtag gcaatttcac ggtcagcatg ggcatttttc agccatcccg ccagtgcgtg
    35161 gatatcctct tcggtaactg agttaccatc ctgccagcgc agcaggtttt cgagcaaaac
    35221 tttgagtgac ttgggtagac gggtgatatc gcccagtgat ttagcagcaa gcggcaggct
    35281 gtagtagtgg taagttttat ctttggcctg caacgtgtcc ttactggctt ctcgtagggt
    35341 tgacgacata gctcctcctt aatgacaggg ttgcgaagcc ctgattctct tcagggtgaa
    35401 tattaaagat aacacaaaca tcgcgtaacg atttgataac aacccaaatt gataaaagag
    35461 gagaaacctt gtggcaaagc aaatgacaac cccgccgcag cggggtcaag gatcagtgag
    35521 taatgatcca gaggagttcg gcccagaaga gcatcgacag agaaaacgcg cccatccagg
    35581 accaatattt catatttgtg ttgatcatag gctacaccct taacggttat tattttgtgt
    35641 tgagataact caagctgtta ttgctgcggt gtattaaaag ccctttaatt cattaaaaat
    35701 gggtaataag aatgatgata attattgttt cggctgatca tcttcagcaa acagttctat
    35761 aaaggcttgc tgctgcgatg ttagttgcca gtcgtctgga ataacgacgg aacattcccg
    35821 tttcgtttct ggttcattgt cactatgctg gcataatggt gacgactcca gttgctcctg
    35881 accgaccata gttatcccca cactttccaa atcagcagtg cgacaaccac ccagaaaagt
    35941 gcagaaccaa gaaaaaccgc cagccaggct ttacgtttta attcaggatc acggcggggt
    36001 tcctgatttc cagaaggcat tgctaacctc atataatcgc catcacttat cagcaagacg
    36061 ttgaatgatt ggtgtaatta atcatacgat gagacaaatc ctaaagaaac tttagtcaaa
    36121 aatccagcga atttacgaat cgtctccagt gaataattca atcttttgac ctgaaataga
    36181 tatttgaaaa ctggaatttg cgaggcgggt aattagacac tactttacag gtaatttacc
    36241 ctataaatat gagggataaa aatattgtgc acggtataga aataagtaac aaaagagaat
    36301 atattccggc accttcgcta cataaaaggt gccgaaaata acgcaagaaa ttatttttcc
    36361 ggcagtttta tatctttaaa catgacctca atttcttcat tggagcgcaa tgcgacagcc
    36421 gcatcaacga catcacgcgt taaatgcggt gcaaaacgct gaatgaaatc atacatataa
    36481 ctgcgcaaaa aagtactacg gcgaaaacca attttggttg tactgtggct gaagatatcg
    36541 tgagcatcaa cgcgcacaag gtcgggatcg gcgaccggat ccaccgccat gctggcaatg
    36601 acccctaccc ccagccctaa ccggacgtaa gttttaatga cgtcagcatc cgttgccgtg
    36661 aaaacgatac gcggcgttaa ccctgcgcgg ttaaaggcag tatccagttc tgaacgtccg
    36721 gtaaagccga aggtatatgt caccaacgga tattgcgcca gttcttcaat ggtaatggct
    36781 tttttgcctg ccagcgggtg atccggagtg actacaatag cccgattcca gtggtagcac
    36841 ggtaacatca ctaaatcttc atacagatgc agcgcttctg tggcgatagc gaaatcggca
    36901 ttgcctttag agacggcatc agcaatttgt gtcggcgggc cctggtgcat atgcaaagaa
    36961 acgcggggat aacgctcaat aaagcctttg ataacgtttg gtaacgcgta gcgtgcctgg
    37021 gtatgcgtgg tggcgatata cagcgagcct ttatccggcc aggtgtgctc accggcaacc
    37081 gattttatgg catcgacttt cgacaggact tcgcgagcga tacgaattat ttcttgtcct
    37141 gctggcgtta cctgcgtcag gtgcttgccg cttcgggaaa aaatttgaat gcctagctcg
    37201 tcttccagca ttctgacttg tttactgatc ccgggttgtg atgtgtaaag tccttccgct
    37261 gttgatgaga cattcaggtt atgattgacc acctcaacaa tatagcgaag ttgttgtaat
    37321 ttcatgttaa accatccact tagacgccat cagaacatcg ttttttcgtc acacctggcg
    37381 ggtgactaat acgatttaat aataaaaaag gtgctaacta taaccactat atcatttata
    37441 gccttagtgt ataggtgtga agaaaaaata atatgcaata aaaaagggcc gctttcgcga
    37501 ccctttgttt ataaaaacct gacagaatta aaggttattt ttttccttca acccatttgc
    37561 catcaacata aaatgctgac cagccagtcg cctttccgtc tttttccgaa gagacatatt
    37621 gctgtttggt tttacggcta aagcgaacca tggtcttatt accttccgga tcctgctgtg
    37681 gcgcatcggc cagataacgc agtttttccg gcagacggtc gcggaagcga taaagctctt
    37741 ccaccagtgg cgcacgcgtt tcacgcgatt tcgggaaagt gttggcagcc aggaacacac
    37801 cggcagcacc gtcacgcagc acgaaataag catctgattt ttcgcacggc agctcaggta
    37861 atggcaccgg atcttctttc ggtggtgcca cttcgccgtt acgtaaaatc ttacgtgtgt
    37921 ttttacactc ttcgttggtg caggccatgt atttaccgaa acgccccatt ttcaggtgca
    37981 tttcagagcc acatttttca cactcaacga tcgggccgtc ataaccttta atgcggaatt
    38041 cgccctcttc gatctcgtaa ccgtcgcagg ttgggttatt accacagaca tgcaacttac
    38101 gtttcggatc gatgagatag ctgtccatcg ccgtgccgca tttcgggcaa cgacgttttg
    38161 cgcgcagcgc gttggtttca gcatcttcgc cttccagcac gttcagcact tcgttttccg
    38221 gcaccaggtt aatggtggtt ttgcaacgct ctttcggcgg cagcgcatag ccagaacagc
    38281 caaggaatac cccggtactc gctgtgcgaa tccccatttt gcgaccacaa gtcgggcagt
    38341 caatgctggt cagaaccatc tggttcgggc gcataccacc ctcttccgga tctttttcag
    38401 ctttatctaa ctgctgggtg aaatccgaga agaagtgatc cagtacagct ttccactctg
    38461 cttcgtgatt tgccacctgg tcgaggctgt tttccatctg cgcggtaaag tcgtagttca
    38521 ttaactcgcg gaaattttct tcaaggcgat cggtgacgat ttcgcccatt ttttccgcat
    38581 agaaacgacg attttctact cgcacatagc cacgatcctg aatggtcgaa atgatcgacg
    38641 cataggtaga cggacgaccg ataccacgtt tttccagttc tttaaccagc gatgcttcac
    38701 tgaaacgggc tggcggcttg gtaaagtgct gggctggtgt tagttcaacg agcgtcagag
    38761 catcgccttt atcgactgcc ggtaagatac gatcttcatc gcctttacgc agtgcaggca
    38821 tcacttttgt ccagccatca aagcacaaaa tacgaccgcg tgctttcagg cggaaatcgc
    38881 ccgcaccaac ggtcagcgtc gtggagtcat atttcgctgg ggtcatctgg caggcaacga
    38941 actgacgcca gattaactgg tacagtttct gcgcatctgc ttccatatcc ttcagcgatt
    39001 ccgccatcac attgacgtca gaaggacgaa tcgcttcgtg cgcttcctgt gagttttctt
    39061 tgctggcgta ctgattcgga ctttccggca gatatttctt accaaaatta tcgctgatat
    39121 aaccgcgaac catatttacc gcgtcctgac tcaggttagt ggagtcggta cgcatgtaag
    39181 tgatatagcc tgcttcatac aaacgctgcg ccatcatcat ggtttttttc acgccaaaac
    39241 caagacgggt gctggcagct tgttgcagcg tagaggtaat aaaaggagcg ccaggtttac
    39301 tggttgtcgg tttgtcttca cgttccagca cgctgtagcg cgctttttcc agcagactta
    39361 ccgcagcctg agtttgttct ttgttgaccg gacggaacgg tttgtcattc tgatgagtca
    39421 cctgcaacgc caacgcttca ccagatggcg tagtcgtgct ggcatcgact tcccagaact
    39481 cttccggtac gaacgcttta atttcacgct cacgctctac caccaggcgg accgccaccg
    39541 actgcacacg accggcagac agaccacgag cgatcttttt ccacagcagc ggcgaaacca
    39601 tataccccac cacgcggtcc ataaagcgac gcgcctgctg ggcattaaca cgatcaatat
    39661 tcagctcacc cggtttgtta aatgcctggc ggatcgcgtt tttagtaatt tcgttaaaca
    39721 ccactcggct atagcgcgca tcatcaccac caatcacttc ccgcaggtgc catgcaatgg
    39781 cttccccttc gcggtcaagg tcggttgcga gatagatgtg gtcggctttt tcagccagtt
    39841 gtttcagttc agagacgacc ttctctttac caggcaacac ttcatagtgc gcctcccaat
    39901 tgtgccacgg gtcaaccccc atacggttga cgagagcgcc acgttcatcc tttttaggct
    39961 ttttagccgt cttggtggag gtagagtcgg cactcttttt ggcagctgag ccactggtcg
    40021 gcaaatcgcg gatgtgaccg acgctggatt tcaccacgta gtcacaaccc agatacttgt
    40081 tgatcgtttt ggcttttgcc ggggactcaa cgatgacaag agctttaccc atattcacct
    40141 tacctaattt gattcttcca ggaatgcgtc gcacgttgat aaaccttcca ctggcaacga
    40201 gttaccgata tggactcgct ctctgcggat atcaacccct tgtcgtgact ctgttcacga
    40261 aaattcaggt gactgagtta acaggtcttg gcctacaggc gctatagcac gacggaattt
    40321 tggtcgaatg tcaagcaaat ctattgccag attgacgaaa gcgtcacact gtacctgata
    40381 aaatccgata cgcaacttta ttagcatgca aaatccaaag ttgccagtaa ggcgctcttg
    40441 ccaccagcgt ggtatatttg tctgcaagcc atgcctggcg tagactaatg tccttgttta
    40501 aggagtaaat aatgaacaaa gagacacaac ccatcgaccg cgaaacgctg ttgaaagagg
    40561 ccaacaaaat cattcgtgag catgaggata cgctcgcagg gattgaagcc accggcgtca
    40621 cgcagcgtaa tggcgtgctg gtatttaccg gcgactactt tttagatgaa caaggattgc
    40681 caacagccaa aagcaccgcg gtgtttaata tgttcaaaca cctggcccat gtgctttcag
    40741 aaaaatatca tctggttgat taaagcaaaa cgcgaggtct tagcctcgcg tttgtctttt
    40801 acatcaatgg cttttgaccc cgctgccacc agcgtagcaa caatcgatcg gcgctctctg
    40861 ccgcgctgcc ggtgaatcgg tcaatgagtc gtttacgctg catatagcgt acattaacca
    40921 cttcacggcc ttccatcagg ctaagaataa cttcatcact ggtgttgatt tcatcaacca
    40981 ggcctttctc taccgcctgt tgtccgtacc agtgttcacc cgttgccacc tgttcaatat
    41041 ccagagacgg acgcatacgc ttcacaaaat ctttaaataa ctgatgcgtt tcgttcagct
    41101 cttcgcggaa tttctcccgc ccttcttcgg tattttcacc cagcaaggtc agcgtgcgct
    41161 tatactgccc ggcggtgtgc agttcgatat caatatcttt gcttttcagg aagcggttaa
    41221 agttgggcat ttgcgccacc accccaatgg aacccacaat agcaaacggt gcggaaacaa
    41281 ttttgtccgc cacacaggcc atcatgtaac cgccgctggc agcgactttg tctaccgtaa
    41341 cagttaaagg aatgttttta tcacgcagac gctgcagctg cgaagccgcc aacccgtaac
    41401 catgcaccat gccaccaggg ctttccagac ggagcacaac ctgatcctgc ggtttgaatg
    41461 ctgcgagtac cgccgttatc tcttcacgta gcgagttcac ttcatgggcg tccatgctgc
    41521 ctttaaaatc cagcacccag acgcgaggtt tactgtcagt tgccacctcg cccagtttgg
    41581 ctttcgcttt tgctgctttc gcttcttgct tgtgcttttt cttctgcgct ttgtgccact
    41641 gtttttgctg atgtgagtcc atcagcgccg cggccagttc ttctttcatc tccttatact
    41701 gttcgctgag attgttgacc cgtaactcgc cacgctggcg tttattacgt tgagcaacat
    41761 tgacaataat ggcggcaatc gccgcaatcg ctagcacaac ggtaacgatt ttagccaaaa
    41821 acaaaccata ttcagacaac aattccacga gtccccacct tggttaaaca acgctttggc
    41881 tcacagttta caacaggcat gttaagccgt ctcgcagaga tgttattgca gcgaaagtat
    41941 gacaaagtat ccattttgcg gtgttatcgc cccaggctga ttgaaatgcc cttctgtttc
    42001 aggcataaag ccccaaagtc ataaagtaca ctggcagcgc gacagacaac gtcgttgagg
    42061 agtagccatg cattaccagc caaaacaaga tttactcaat gatcgcatta tcctggtgac
    42121 gggagccagc gatgggattg gtcgtgaagc cgcgatgacg tatgcacgct atggtgcgac
    42181 agtgattctg ttgggccgta atgaagaaaa attacgtcag gtagccagcc acataaacga
    42241 agaaactggg cgtcagccac agtggtttat tctcgatttg ctgacctgca cgtccgaaaa
    42301 ttgccaacaa ctggcacagc gcattgtcgt taattatccg cgtctggatg gtgttttgca
    42361 taatgccgga ttgctcggcg atgtttgccc aatgagcgaa caaaatccgc aggtctggca
    42421 ggacgtcatg caggtcaacg ttaatgccac ctttatgctc acccaggcac tgcttccttt
    42481 attactcaaa tcggacgccg gttcactggt ctttacttca tcaagcgttg gacgtcaggg
    42541 acgagccaac tggggtgcat atgcagcgtc gaaatttgcc accgaaggga tgatgcaggt
    42601 actggccgat gaatatcagc agcgcctgcg cgtcaactgc attaacccag gcggtacgcg
    42661 caccgcaatg cgcgcaagcg ccttcccgac cgaagatcca cagaaactta aaacacccgc
    42721 tgatatcatg ccgctctacc tctggctgat gggcgatgac agccgccgta aaaccggcat
    42781 gacctttgac gcccaaccgg gccgtaaacc aggaatttcc caatgagtga tgaacgctac
    42841 caacagcgtc agcagcgagt gaaagaaaaa gtagatgctc gtgtggccca ggcccaggat
    42901 gaacgcggta ttatcatcgt ctttaccggc aatggaaaag gcaaaaccac cgcggcattt
    42961 ggtacggcaa cacgcgcagt tggtcacgga aaaaaagtgg gcgtcgtgca gtttattaaa
    43021 ggcacctggc ctaatggcga acgcaatctg ctggagccac atggcgttga gtttcaggtg
    43081 atggcaacgg gctttacctg ggatacacaa aaccgcgagt ctgataccgc cgcctgccgc
    43141 gaagtctggc aacatgcaaa gcggatgctt gctgattcct cactggatat ggttttgctt
    43201 gatgaactga cgtatatggt ggcgtatgac tatttgccac tggaagaagt ggtgcaggcg
    43261 ttaaatgaac gtccacatca acagacggtg attatcacgg gtcgaggctg tcatcgggat
    43321 attcttgaac tggcggatac ggtaagtgag ctgcgcccca tcagacatgc gtttgatgcc
    43381 ggtgttaagg cgcaaatagg gattgattat taatctgtaa aaatgccgga tatgacgtcc
    43441 ggcatacttt gattaaccgt tattacgccc gccagaacga cgactgccgc tcacctgact
    43501 gtgacgtttc accgcacgac gaatctgatt agccttcatg cgacgacggt ctttttctac
    43561 agcgacttta gagctggttt ctggcggaag ctccaccagt tcgcgcagat agttagtctg
    43621 ggcgaggtcc agctccgtcc agccaccgcg tggcagacct tttggcagtg ggatatcacc
    43681 gtaacgaaca cggatcaggc ggctcacctg cacaccaacc gcttcccaca gacgacgaac
    43741 ttcacggtta cggccttcgg tcagagtcac gttgtaccac tggttgatcc cttcgccgcc
    43801 gctgaacttg atggttttaa aagctgccgg accatcttcc agttgcacgc cacgactcaa
    43861 atcacgcagt ttcgcgtcgt caacctgacc aaatacacgc acggcgtatt cacgttcaac
    43921 ttcacggctt ggatgcatta aacggtttgc cagttcacca tcggtggtga acagcagcag
    43981 accacaggtg ttaacgtcca gacgacctac ggcaatccag cgtgcgccac gcagctttgg
    44041 cagacggtca aacaccgtcg gacgcccttc cggatcgtta cgggtacaca actcaccttc
    44101 cggtttgtaa taggccagaa cgcgacaaat ttgttcagcg gactcacgta ccgaaatcag
    44161 atgaccatcg atacgaattt tcagcccagg ggtaacttca acgcgatcgc cgagtttagc
    44221 aattttgcca tcaacactca cacgaccggc ttcaataata gattcgattt cacggcggga
    44281 gccgtggcca gcgcgggcca gcactttctg taatttttcg ctcatagagc ttccttttgg
    44341 tgtcgccttc acaggcgtcg aacaggagaa tcaatggcgc cgtatagcgc cattttaagg
    44401 ccgcgtagta tacagagtta cgcacttata agaaaggctt cacatcacct acgccttcac
    44461 gcaccacgac cggcgtatca tcggtaagat caataaccgt tgttggtttc tgcccgagat
    44521 agccgccgtg aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat
    44581 cggattcggt aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca
    44641 gtgcttcaag caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc
    44701 gtttctcctg caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt
    44761 tgcccggcgt gttgtttttc atcaaacgaa acgcaacgtt atccacgaat gaataggtcg
    44821 agagttcaga aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa
    44881 tacgacaaat acgctccatg gcgtttttat cttcaatttt acagccgagc gcatagccgg
    44941 aatcagttgg atagacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca
    45001 gacgttgctg cgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt
    45061 ggtgttctgc ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag
    45121 tttacgaccc agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca
    45181 taaatgatgc tgacgcgcaa gtgtggccag ctgggtgcgt tcattgggcg attgctggca
    45241 ctgcgcgact tccatcgcgt cgccgtggtg ttcggcaaaa tgcgcaacca atcttttcag
    45301 ccatttagca ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg
    45361 atgaatgaca tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt
    45421 cccgcgcgcc agatactttt taaagacatc tgccattgaa ctggctttgc cgcactccac
    45481 aaggaaacgc gcaaaatgac cgcgcgtcac tgcgccgccc tgcgccagtc gttgtgcgcc
    45541 ttccagcgcc ccaggtattt gcgctttttc aagtctttcg gcaatttgct gcgcccgctg
    45601 attgcgccgt tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat
    45661 attcagcccg acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg
    45721 aataagattc agcgccagtc ccgaacgtga aatttcctct cttgctggcg cgattgcagc
    45781 tgtggtgtca tggtcggtga tcgccagggt gccgacgcgc atctcgactg cacggtgcac
    45841 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat
    45901 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg
    45961 acatcataac gggtctggca aatattctga aatgagctgt tgacaattaa tcatcgaacc
    46021 agttaactag tgcgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac
    46081 tgaaaggttg gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc
    46141 agatacccag cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg
    46201 caaacacaaa aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg
    46261 actgcgcttt ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca
    46321 gatatcgaca gcaaagatga tttaaaaagc ctgctgctga tagacagtgc gctgcgcatt
    46381 acagcattag gtgacaccgt cacaattcag gcactttccg gcaatggcga agccctgctg
    46441 gcactactgg ataacgccct gcctgcgggt gtggaaagtg aacaatcacc aaactgccgt
    46501 gtgctgcgct tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt
    46561 tcggtttttg acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga
    46621 gaagcaatgt tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta
    46681 ccgcaactgt cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg
    46741 atggtgattg accatcagaa aaaaagcacc cgtattcagg ccagcctgtt tgctccgaat
    46801 gaagaagaaa aacaacgtct cactgctcgc ctgaacgaac tacgtcagca actgaccgaa
    46861 gccgcgccgc cgctgccagt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc
    46921 gatgaagagt tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt
    46981 ttccaggtgg tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat
    47041 tacgtgctga aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc
    47101 accctatttg gcgcgtcgcc ggaaagctcg ctcaagtatg atgccaccag ccgccagatt
    47161 gagatctacc cgattgccgg aacacgccca cgcggtcgtc gcgccgatgg ttcactggac
    47221 agagatctcg acagccgtat tgaactggaa atgcgtactg accataaaga gctgtctgaa
    47281 catctgatgc tggttgatct cgcccgtaat gatctggcac gcatttgcac ccctggcagc
    47341 cgctacatcg ccgatctcac caaagttgac cgttattcct atgtgatgca cctcgtctct
    47401 cgcgtagtcg gcgaactgcg tcacgatctt gacgccctgc acgcttatcg cgcctgtatg
    47461 aatatgggga cgttaagcgg tgcgccgaaa gtacgcgcta tgcagttaat tgccgaggcg
    47521 gaaggtcgtc gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat
    47581 ctcgacacct gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa
    47641 gcgggtgctg gtgtagtcct tgattctgtt ccgcagtcgg aagccgacga aacccgtaac
    47701 aaagcccgcg ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga
    47761 tggctgacat tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt
    47821 tgcgcagcaa tggtcataac gtggtgattt accgcaacca tattccggcg cagaccttaa
    47881 ttgaacgcct ggcgacgatg agcaatccgg tactgatgct ttctcctggc cccggtgtgc
    47941 cgagcgaagc cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta
    48001 ttggcatttg cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg
    48061 cgggcgaaat tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg
    48121 ccggattaac aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc
    48181 cggccggttt aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg
    48241 cggatcgcgt ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc
    48301 gcctgctgga acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc
    48361 aaccgattct ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc
    48421 tgttttcagc ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga
    48481 gcatgaaaat tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg
    48541 aaaacgcagc gccgttcccg cgcccggatt atctgtttgc tgatatcgtc ggtactggcg
    48601 gtgacggcag caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gcggcctgtg
    48661 ggctgaaagt ggcgaaacac ggtaaccgca gcgtctccag taaatccggc tcgtcggatc
    48721 tgctggcggc attcggtatt aatcttgata tgaacgccaa taaatcgcgc caggcgctgg
    48781 acgagttagg cgtatgtttc ctctttgcgc cgaaatatca caccggattc cgccacgcga
    48841 tgccggttcg ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta
    48901 acccggcgca tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga
    48961 ttgccgaaac cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga
    49021 tggatgaagt ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcaaaatta
    49081 agagctatca attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg
    49141 caggcggaac acctgaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg
    49201 acgccgccca tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc
    49261 atgaagatct gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt
    49321 acgacagagt taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa
    49381 tcgtcgcaga caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt
    49441 ttcagaatga ggttcagccg agcacacgac atttttatga tgcgctacag ggtgcacgca
    49501 cggcgtttat tctggagtgt aaaaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt
    49561 tcgatccggc acgcattgcc gccatttata aacattacgc ttcggcaatt tcggtgctga
    49621 ctgatgagaa atattttcag gggagctttg atttcctcct catcgtcagc caaatcgccc
    49681 cgcagccgat tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct
    49741 attaccaggc cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc
    49801 agcttgcagc cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag
    49861 aggaactgga gcgcgccatt gcattagggg caaaggtcgt tggcatcaac aaccgcgatc
    49921 tgcgtgattt gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca
    49981 acgtaacggt aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc
    50041 acttcgctaa cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg
    50101 ccgtgcgccg ggtgttgctg ggtgagaata aagtatgtgg cctgacgcgt gggcaagatg
    50161 ctaaagcagc ttatgacgcg ggcgcgattt acggtgggtt gatttttgtt gcgacatcac
    50221 cgcgttgcgt caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg
    50281 ttggcgtgtt ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc
    50341 tggcggcagt gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag
    50401 ctctgccagc acatgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc
    50461 gcgagtttca gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac
    50521 gttttgactg gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct
    50581 taggcgcaga taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt
    50641 ctgctgtaga gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga
    50701 cgctgcgcgc atattaagga aaggaacaat gacaacatta cttaacccct attttggtga
    50761 gtttggcggc atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc
    50821 ttttgtcagc gcgcaaaaag atcctgaatt tcaagcccag ttcaacgacc tgctgaaaaa
    50881 ctatgccggg cgtccaaccg cactgaccaa atgccagaac attacagccg ggacgaacac
    50941 cacgctgtat ctcaagcgtg aagatttgct gcacggcggc gcacataaaa ccaaccaggt
    51001 gctggggcag gcgttgctgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg
    51061 ggccggtcag catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg
    51121 tatttatatg ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt
    51181 aatgggtgcg gaagtgatcc cggtacatag cggttccgcc acgctgaaag atgcctgtaa
    51241 tgaggcgcta cgcgactggt ccggcagtta tgaaaccgcg cactatatgc tgggcaccgc
    51301 ggctggcccg catccttatc cgaccattgt ccgtgagttt cagcggatga ttggcgaaga
    51361 aaccaaagcg cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt
    51421 tggcggcggt tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg
    51481 cctgattggt gtggagcctg gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct
    51541 aaaacatggt cgcgtgggca tttatttcgg tatgaaagcg ccgatgatgc aaaccgaaga
    51601 cgggcagatt gaagaatctt actccatctc cgccggactg gatttcccgt ctgtcggtcc
    51661 acaacacgcg tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga
    51721 agcccttgaa gccttcaaaa cgctgtgcct gcacgaaggg atcatcccgg cgctggaatc
    51781 ctcccacgcc ctggcccatg cgctgaaaat gatgcgcgaa aacccggata aagagcagct
    51841 actggtggtt aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt
    51901 gaaagcacga ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag
    51961 cgcaaagaag gcgcattcgt tcctttcgtc acgctcggtg atccgggcat tgagcagtca
    52021 ttgaaaatta tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggtatcccc
    52081 ttctccgacc cactggcgga tggcccgacc attcaaaacg ccactctgcg cgcctttgcg
    52141 gcaggtgtga ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg
    52201 accattccca ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag
    52261 ttttatgccc agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccagtt
    52321 gaagagtccg cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc
    52381 tgcccgccaa atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac
    52441 acatatttgc tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc
    52501 aatcatctgg ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt
    52561 atttccgccc cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct
    52621 ggttcggcca ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg
    52681 gcactgaaag tttttgtaca accgatgaaa gcggcgacgc gcagttaatc ccacagccgc
    52741 cagttccgct ggcggcattt taactttctt taatgaagcc ggaaaaatcc taaattcatt
    52801 taatatttat ctttttaccg tttcgcttac cccggtcgaa cgtcaactta cgtcattttt
    52861 ccgcccaaca gtaatataat caaacaaatt aatcccgcaa cataacacca gtaaaatcaa
    52921 taattttctc taagtcacat attcctcagg taattgttaa tatatccaga atgttcctca
    52981 aaatatattt tccctctatc ttctcgttgc gcttaatttg actaattctc attagcgact
    53041 aattttaatg agtgtcgaca cacaacactc atattaatga aacaatgcaa cgcaacggga
    53101 gaaataacat ggccgaacat cgtggtagtt caggaaattt cgccgaagac cgtgagaagg
    53161 catccgacgc aggccgtaaa ggcggtcagc atagcggcgg taattttaaa aatgatccgc
    53221 aacgcgcatc tgaagcgggt aaaaaaggcg gtcaacaaag cggtggtaat aaatcaggca
    53281 aatcctgatt caccgttaat tattaatatc agctgataaa gataattgca tctgcgggcc
    53341 gtatggctcg cagtgatttc actggagaaa aatatgaata tgaagaccat tgaagatgta
    53401 tttattcaac tgctttcaga tacctacagc gcagaaaaac aattaacccg ggcactggca
    53461 aaactcgcaa gagcaacatc aaatgaaaaa ttaagtcagg cttttcatgc gcacctcgag
    53521 gaaactcatg gacagattga acgaattgat caagttgtgg aatcagaatc aaatctgaaa
    53581 attaagcgca tgaaatgtgt ggcaatggaa ggtcttattg aagaagctaa tgaggtcatc
    53641 gaaagtaccg agaaaaacga agtgcgtgat gccgcactga ttgccgcagc acagaaagtc
    53701 gagcattatg agattgccag ttacgggaca ttagcgacgc tggctgaaca attaggttac
    53761 cgtaaagcag cgaagcttct gaaagaaacc ctggaagaag aaaaggccac cgacatcaaa
    53821 ctgactgatc tggcccttaa taacgtaaat aagaaagccg aaaataaagc ctgaaatatg
    53881 aattttaact tttagtcatt ttataaagag gacattttca tgaatcgtat tgaacattat
    53941 catgactggt tacgtgacgc ccacgcaatg gaaaagcaag ccgaatctat gcttgaatcc
    54001 atggccagcc gtatagataa ttatcctgaa ctacgcgctc gtattgaaca acatcttagt
    54061 gaaaccaaaa accagattgt tcaactggaa actattcttg atcgtaatga catttcacgt
    54121 tcagtcatta aagattccat gagtaaaatg gctgcgcttg ggcagtcaat cggtggtata
    54181 ttcccttctg atgaaatagt taaaggctct attggcggat atgtcttcga gcaatttgaa
    54241 atcacctgtt acacctcact attagcagca gcaaaaaatg ccggtgatac agtctcaatt
    54301 ccgatcatcg aagcgatttt aaatgaggaa aagcaaatgg ccgactggct gattcagcat
    54361 attccgcaaa caactgagaa atttttaatt cgttctgaaa ctgatggcgt agaagcgaag
    54421 aaataatacc ctttatacca tgtccttatt gaccccgtat attacggggt cgtttttgtg
    54481 cggaattaaa aacgatatcc tgctgagaac ataaacaccc acggatcgag gcgtacgcta
    54541 tcgtgttgct gtgcaccgcc tagcttataa ttggcggtgg tatcgatatc catgtaccac
    54601 actgacatgt taaccaacca gtcacggtta atcagataat caacccccac ctgcccggca
    54661 gctccccagg aatctttcag actgagatcg gaaagccccg cctctttgcc atgatcgtta
    54721 aatccattat caaagaaggt ggtgtagtta ataccggccc caacgtaagg acggaatttg
    54781 ctgctggcat caccaaaata ccactgcgcc atcagtgttg gcggcagatg gtgaacggtt
    54841 gcaatatcgc cggtcgcccg ggtgccgatt ttatggcgga acggcgtcgc tgccagtaat
    54901 tccacaccaa tgttgtcggt cgccatataa gtaaacgtca agcccagttg agtgttattg
    54961 gtcacgctga atccacccag acttcctaac gtaccaccag caccttctgt tggacgtacg
    55021 gttgcagaac ctgcacgcat aaaaaattcg cctgcttcat gcgcaaaggc actgccagaa
    55081 agaagagttg ttactgccaa agccgccact gttaactttt tcatatccgc tccgtcgtta
    55141 tggttataaa aacgtgacga atatacctac attggagtaa caaatgatcc tacatagatc
    55201 acattacata tgataattca ccatttattg atctggatta atttagaagt tgcattgaga
    55261 atcctggtta atgtgattta aatcaatttt taaatatttt ctctacgcaa atattaactc
    55321 gatcgccact tgccgcggtt tgcccacgcc tgaaggccag caattacctc cgctttacaa
    55381 agatatgctt ttccatacaa gacctgatgc tgccacccga ggcttaacca gggtacaatt
    55441 gcccgctaat taacacctgc ataaaactca aggagagtgc atgtctatca cggcgcagtc
    55501 cgtataccgt gacaccggaa atttcttccg taatcaattt atgaccattc tgttggtatc
    55561 gttgctatgt gcgtttatca cagtggtgtt agggcatgtt ttctcaccca gtgatgcaca
    55621 gcttgcgcag ctcaatgacg gcgtgcccgt tagcggcagt agtgggttgt tcgacctggt
    55681 tcagaatatg tcaccggaac agcaacaaat tttgctgcag gcttcagcgg cgtccacttt
    55741 ttcagaatta atcggtaacg ccattctcgc cggaggcgta atattaatta tccagctggt
    55801 gtctgcgggt cagagagtca gtgcgctgcg agctattggt gccagtgcgc cgatattgcc
    55861 caagttattt attctgattt ttctgactac ccttttagta cagattggca tcatgctggt
    55921 ggtcgttccg ggaattatca tggccatttt actggccctg gcaccggtga tgctggtaca
    55981 ggacaaaatg ggcatttttg cctcgatgcg tagcagtatg cggctgactt gggcgaatat
    56041 gcgtctggtg gcacccgcag tactgagctg gttgctggca aaaacactgt tgctgctttt
    56101 tgcctcttct tttgccgcat taaccccgga aattggtgcc gtactggcga acaccttgag
    56161 caacctgatt tctgccatat tgctcatcta tctgttccgc ctgtatatgt tgattcgcca
    56221 ataaccttaa gcatttgatt acggaatcgt aaaatgaagc agtttcttga ttttttaccg
    56281 ctggttgtct ttttcgcgtt ttacaagatt tatgacatct atgcggctac tgcagcgctg
    56341 atcgtcgcca cggcgattgt gcttatctat agctgggttc gctttcgtaa ggttgagaag
    56401 atggccctga tcacttttgt tctggtggtc gtcttcggtg gcttgacgct gttcttccac
    56461 aatgatgagt ttattaaatg gaaggtgaca gtcatttatg ctctgtttgc gggagccctg
    56521 ttagtcagcc aatgggtgat gaaaaagccg ctaattcagc ggatgctgag taaagaactc
    56581 acgctgccgc aaccggtatg gtcgaagcta aatctggcct gggctgtttt ctttatcctt
    56641 tgcggtctgg caaacatcta catcgcattc tggctgccgc aaaatatttg ggtcaacttt
    56701 aaagtctttg gcctgaccgc ccttacctta atcttcacat tgttaagcgg tatctatatc
    56761 taccgccaca tgccgcagga agataaatcc taaccagact gccagtcctt cggggctggc
    56821 gctgtatctc ctctgttctc ctcaggaaaa tcatagtagc atcgcgcctg tgattttcct
    56881 tttaagtcgg ttttaccatg tctacaacac ataacgtccc tcagggcgat cttgttttac
    56941 gtactttagc catgcccgcc gataccaatg ccaatggtga catctttggt ggttggttaa
    57001 tgtcacaaat ggatattggc ggcgctattc tggcaaaaga aattgcccac ggtcgcgtag
    57061 tgactgtgcg ggttgaagga atgactttct tacggccggt tgcggtcggc gatgtggtgt
    57121 gctgctatgc acgctgtgtc cagaaaggga cgacatcggt cagcattaat attgaagtat
    57181 gggtgaaaaa agtagcgtct gaaccaatcg ggcaacgcta taaagcgaca gaagcattat
    57241 ttaagtatgt agcggttgat cctgaaggaa aacctcgcgc cttacctgtt gagtaatagt
    57301 caaaagcctc cggtcggagg cttttgactt tctgcttact gaatttcggt ggtgccgtta
    57361 attttaaaca ggatattcac cacaatccca ctgcctggct tacccggctc ataacgccat
    57421 ctgcgcatcg catttttcac ctcacgctca aacatgttcg caggcttggc tgagaggatt
    57481 tgtacgttat ccacgcgacc atccggcgtg acgtcaaatt taactttaac ctgcccttca
    57541 atgcgcaatg cctgtgctcg tgccggatac tgcggctgat tacggcttaa tgcgcgtggt
    57601 cctgaagcca cactggtaac cggcttgctg gttgcagccg ttgccgtact tgatgtgggg
    57661 cgcgcgggtg ccgtattttc aaacggtgat gccggacgcg actctacggg tttgacatcg
    57721 cgttttggct gctcctgtac ctttttcacc ggcttcggtt ttggcttcgg tttcggcttc
    57781 ggcttttcaa tgaccaccgg tgcttctttt ggcggttcgg ggatcggctc aggttccggt
    57841 tctggctcta ccaccggctc cggtggcggc tgaacggctt gtggcggttc gagatcagca
    57901 ggcgcaacca tcgtgacaga aattggttgc gcaggcgcag gtagttcaat aacctgatgt
    57961 accgaggtat agagcagccc cgccacaaca gcaccatgaa tgcagaccga aagtaacgtc
    58021 ggccagggga agcggcgagg taaatcaagg gttattgaag tcataatcat ttcagtagaa
    58081 aaactaggcc tcgattttaa atgcaaatag caatcatatt caataaggca agacgatcag
    58141 gatcagtgaa aatgtcattt ttggcgtaat tacccttact ttatgcatga ccttaacaaa
    58201 gacatcatct ttccgttgca gatcttcgcc ctttcaaata acgtacttta caactttccc
    58261 gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc aagacaaagc tgatagcctc
    58321 gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt tacagttact gcatgatgaa
    58381 ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca gtaacgatcc aggggctgcg
    58441 ggatttactg gctcaaccgt tattgctgaa tttgaatcgc tggaagcagc tcaggcctgg
    58501 gccgatgctg acccgtacgt agcggcagga gtctatgaac acgtttcagt gaaaccattt
    58561 aagaaagtgt tctgag
//
LOCUS       DAJVRL010000012        58047 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVRL010000012 DAJVRL010000000
VERSION     DAJVRL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.012"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(24..278)
                     /locus_tag="OQN02_000730"
     CDS             complement(24..278)
                     /locus_tag="OQN02_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1641642.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OQN02_000731"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OQN02_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1641643.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OQN02_000732"
     CDS             1228..2694
                     /locus_tag="OQN02_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1641644.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OQN02_000733"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OQN02_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1641645.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OQN02_000734"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OQN02_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1641646.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OQN02_000735"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OQN02_000735"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1641647.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OQN02_000736"
     CDS             5709..7265
                     /locus_tag="OQN02_000736"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1641648.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OQN02_000737"
     CDS             7347..9161
                     /locus_tag="OQN02_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1641649.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OQN02_000738"
     CDS             9162..10256
                     /locus_tag="OQN02_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1641650.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OQN02_000739"
     CDS             10256..11281
                     /locus_tag="OQN02_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1641651.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OQN02_000740"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OQN02_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1641652.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OQN02_000741"
     CDS             complement(12876..13220)
                     /locus_tag="OQN02_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641653.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OQN02_000742"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OQN02_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCS1641654.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OQN02_000743"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OQN02_000743"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1641655.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OQN02_000744"
     CDS             15616..17376
                     /locus_tag="OQN02_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1641656.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OQN02_000745"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OQN02_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1641657.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OQN02_000746"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OQN02_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1641658.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OQN02_000747"
     CDS             19113..19340
                     /locus_tag="OQN02_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1641659.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OQN02_000748"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OQN02_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1641660.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OQN02_000749"
     tRNA            21195..21271
                     /locus_tag="OQN02_000749"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OQN02_000750"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OQN02_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCS1641661.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OQN02_000751"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OQN02_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1641662.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OQN02_000752"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OQN02_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1641663.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OQN02_000753"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OQN02_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1641664.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OQN02_000754"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OQN02_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1641665.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OQN02_000755"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OQN02_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1641666.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OQN02_000756"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OQN02_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1641667.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OQN02_000757"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OQN02_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1641668.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OQN02_000758"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OQN02_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1641669.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OQN02_000759"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OQN02_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1641670.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OQN02_000760"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OQN02_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1641671.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OQN02_000761"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OQN02_000761"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1641672.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OQN02_000762"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OQN02_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1641673.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OQN02_000763"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OQN02_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1641674.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OQN02_000764"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OQN02_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1641675.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OQN02_000765"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OQN02_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1641676.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OQN02_000766"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OQN02_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1641677.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OQN02_000767"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OQN02_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1641678.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39347)
                     /locus_tag="OQN02_000768"
     CDS             complement(38328..39347)
                     /locus_tag="OQN02_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCS1641679.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39581..39940)
                     /locus_tag="OQN02_000769"
                     /pseudo
     CDS             complement(39581..39940)
                     /locus_tag="OQN02_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="OQN02_000770"
     CDS             complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="OQN02_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1641680.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="OQN02_000771"
     CDS             complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="OQN02_000771"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1641681.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42527..43591)
                     /gene="ada"
                     /locus_tag="OQN02_000772"
     CDS             complement(42527..43591)
                     /gene="ada"
                     /locus_tag="OQN02_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1641682.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="OQN02_000773"
     CDS             complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="OQN02_000773"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1641683.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="OQN02_000774"
     CDS             complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="OQN02_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1641684.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46691..49363
                     /gene="rcsD"
                     /locus_tag="OQN02_000775"
     CDS             46691..49363
                     /gene="rcsD"
                     /locus_tag="OQN02_000775"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1641685.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49380..50030
                     /gene="rcsB"
                     /locus_tag="OQN02_000776"
     CDS             49380..50030
                     /gene="rcsB"
                     /locus_tag="OQN02_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1641686.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="OQN02_000777"
     CDS             complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="OQN02_000777"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1641687.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53354..54130)
                     /locus_tag="OQN02_000778"
     CDS             complement(53354..54130)
                     /locus_tag="OQN02_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS1641688.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54135..55784)
                     /locus_tag="OQN02_000779"
     CDS             complement(54135..55784)
                     /locus_tag="OQN02_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1641689.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55785..56344)
                     /locus_tag="OQN02_000780"
                     /pseudo
     CDS             complement(55785..56344)
                     /locus_tag="OQN02_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56503..>58047)
                     /locus_tag="OQN02_000781"
     CDS             complement(56503..>58047)
                     /locus_tag="OQN02_000781"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1641690.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMPERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14198 a        15658 c        14266 g        13925 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagagta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgaact ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatcttt tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgtgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcggcgtac tatctgctgc ttgcggtttg
    38401 tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca
    38461 tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc
    38521 gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg
    38581 gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc
    38641 acttcggtac ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca
    38701 cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc
    38761 aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga
    38821 tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc
    38881 atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca
    38941 aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt
    39001 ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga
    39061 ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga
    39121 atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg
    39181 gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta
    39241 tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc
    39301 ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt
    39361 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
    39421 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
    39481 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
    39541 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
    39601 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt
    39661 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt
    39721 tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct
    39781 ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
    39841 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
    39901 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
    39961 agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat
    40021 ttaaataatg tttataaatt atattctaat tttagaaaca ttataatttc gttgattaat
    40081 tatagggtta ttagaaatga agggattttt agcatattga cctgacggca gcagtccgcc
    40141 aggtcagcgt cgtaatatta tgccgtccgg gcaacggcat cacgcgaagc ggcatcgcgc
    40201 tcttcgcccg tcagctcgct aagttgccca ttgcgcattt ccagcaggcg gtcggcgtgg
    40261 ataaagtaat gatcatcatg actgatagcg aaaatagttt tacccatctc ctgcatcagc
    40321 ggcagcaaca cctgataaaa ctcacgacgg aagtgtggat cctgatccac cgcccattca
    40381 tccagcagga taatatcgcg ttcttctgcc agcgccagca acagcgccac gcgttttttc
    40441 tgcccttttg ataacttcag gttaacaata cgcccgttgc ttaactcaag cttatgagcc
    40501 attttcagct gcgccagcca cttctcaacc agttgcgggt tagcgggttg cccctccggc
    40561 cccagcagtt gatcaaacag ccagacatcg gtaaacactg ccgaaaacag tttgcgataa
    40621 tcttccggtt gttcgccgct gacaggtttg ccatccagca agatttcgcc gctttgtggc
    40681 tgatacaagc ccgtcaacaa catcgccagc gtcgattttc cgctaccgtt accgccaatc
    40741 agaaacagca gctcgccacg tttgatggtg agattaatcg gaccaacgga aaacgcgtta
    40801 tcctgataag caaacgtcac gttacgcagc tccagcgttt gccagttggg aaacgcctgc
    40861 gggcgcggaa actcagcttt gaaaggcgcg agcgcgaatt tgttcagctt gttaaacgcc
    40921 acctgcgccg tcagcagcgt cggcaatgcg ccaaccgccg aaagcagcgg cgtacgcagg
    40981 aataacagcg tcaacgaata ggtcgcagca acgttggtat cagcccaacc gagactgttc
    41041 gccatccaga acaccaggcc gatagcgccc agcatcatga tgtttgacca gttcacggca
    41101 ctaagatgga aggtgtctgc gcgaataata tgatggcgat actcttgcgc atcggggatg
    41161 tagaggttgt taaacacata ctcggcgcgt tcccggttca gagtcagctc tttgcgccct
    41221 tcaagtacag tttgaaaatc cgtgtacagc ttgtcttcgg tttcacgtag ggtcgccata
    41281 tgtttgtaca cccgcgccac cagcacaaaa ccgccccaga tggtgatcgc catccagata
    41341 gccgttacca gcaacatttt gcccgacagc atccacagat acgccgatga accgatagtg
    41401 agaatgatcc cctgccccag ttccggcaga cgcacaaaag caatggtgat attgcgcaca
    41461 tcgctggtta atcccgccag caacgaggcg ctaccgagtt gttcaatgcg ctcgacgtgg
    41521 gtatccagaa tccgcttgat aaattcacta cgcagtcggt aaacgaagtg atgccccaaa
    41581 gtggtgagcg ccagttgcga tccgagagtg actgccatca acagcagcaa taatcccaga
    41641 aactccggta acaccagcag actggtatcc gccgtttcga taaggcgctg attgataaaa
    41701 gcaattaagc caatgcctaa tgccgcactg gcgaggctta gcgccatcac actgataaat
    41761 ggccagcgat actgccgcca gacaagtaca agaagttcca tgcagaaaac ccggacaatg
    41821 aattacagcc cgcagtttaa acatcttcgc gcgcacagca ataataattc ttatttttat
    41881 tcttttttac ctgcctgacg gaatgtcagg ttgtagcggc agtcggtggt gagtggatga
    41941 aaccccgctt tcaacggttg aataccgtga taaaacagcc gcgattcacc gccccatacc
    42001 accacatcgc catgttccaa caacaaacgt ttgagcggat cattacgttt caggccacca
    42061 aattgaaaaa tcgcgggtaa gcccagagaa acagaaacaa ttggcgcgcg cagatccggt
    42121 tcgtctttat cctgatgcag cgacagtttc gcgccaggag cgtagcggtt gataagacaa
    42181 gcatctggct ggaaatctgg atagcccgcc gccgtagccg cacgttgaca taaatcatga
    42241 aagctttgtg gcatggcggg ccacggttta tttgtttgcg gatcaatggg cgaatagaga
    42301 taaccttgcc gatgggtcgt ccagcccaga tgcccacagt tggtcatcgc caccgacatg
    42361 gtatatccgc ctggagcgac catctggcga aacggcgact ggctggcaac gtcattaata
    42421 tcgcggatta gttgctccgc agcattaaaa gcaaaacgcc gtaaaattac cgcaccagcc
    42481 gccagtggct cttgccacgg ttcagcatcg gcaaacagat ccaacattac ctctcctcat
    42541 tttcagcttc gcggcgcagc agttgcgctt tacgcgacac gccccagcgg taaccggaaa
    42601 gtgtgccatc accacggacc actcgatgac agggtattac gatagccagc ttgttggcgg
    42661 cacaggcgct ggcaacggcc cgtaccgctt tcggtttgcc gatggcgtta gccagttgct
    42721 gataactgac ggtttcacca caaggtatcg tgcgcagtgc ctgccagact tgttgctgaa
    42781 aagcagtgcc gcgaatatcc agcggtaacg tcagcggcgt atcgcgttga ttgaggctgg
    42841 cgatcacttc acgcacatgt tgctgaaacg tcagatcggc aggcgcgttg tcggcagcgg
    42901 gaaacatctg ctgcaactcg ctgattaatg tcgcgtcatc atcgcccagc aatatcgcgc
    42961 aaatcccccg ctcgctttct gccaccaggc aacgacccag ctcacaatca gccagcgcgt
    43021 aacgcaccgc cagattttcg ccaccgtgac ggaattgttt agccgtcatg cccagcgttt
    43081 catcggcttt gcggtagtaa ctgctgctgt cggggaatcc ggcgttaaga atagacgtcg
    43141 tcacgctctc ccctttcgcc agcgattcgc gcaaacggcg agcgcgccag gcctgttgcc
    43201 aggctttagg cgtcattccg gtagtagctt taaacaaccg atgtagatga aatggactca
    43261 tcgccacctg gtcggctaag gcttccagcg ttacaggcgt ttcctgttcc agcagtcgac
    43321 acgcgtgggt gattttatcc aaccgatgtt gccggggatt ggctttgtct ggctgacaac
    43381 gtttgcaggg gcgaaatccg gcggcgagtg cctcgctggc atttgcgtag aaggagacat
    43441 tttcccgcaa ggcatgtctg gcgcggcaag aaggacggca aaagataccc gtggtgcgca
    43501 cggcgaaaac gaattcgccg tcggcattcg ggtcgcgggc taagacagat tgccagcgtt
    43561 gatcgtcagt taagcatgtg gcgtttttca taatcagctc cctggttaag gatagccttt
    43621 agtctgcccg gtcaccatca cgcaaaaacc aacaatcttg cgctttaatt tttttcgctg
    43681 acaaggaagc ttttaaactg tggtgacatc caggttttaa agctatcgcc ttctttggtg
    43741 atcatataaa ccgccagccc ttcccggcga acaacctctt tggctttctc cggcccgagt
    43801 accatcaagc cagtatccca ggcatcggct tccagcgccg tcggagcaat caccgtcacg
    43861 gataccagat tgtgttcgat gggacgcccg gtttgcggat cgataacatg ggaaagacgt
    43921 ttgccgtcca gttcgtaata gttacggtag ctgccagagg tgctaatccc atgaccgttg
    43981 atatccacca cagcctgaac cgcgttttct ttatcggttg gtttttgaat cgctacccgc
    44041 cacggctggc cttcaccgtt cataccacgg ctgttcagcg cgccgcccac cgacaccaga
    44101 tagcgggaaa tcccttcctg ctccatcaag cgtgccaggt gatccgccgc ataaccttca
    44161 ccgacggtgg agagatcgac atataaatcc ggcaggtctt tttgcagata ttgctgatgc
    44221 gactgattga tgaccgtcag gtgctgtaag ccggttttgg ctttcatcgc atcgatctgt
    44281 tcctggctcg gaatttgaac cggctgttgt tccgggccaa agccccacag attcaccagc
    44341 ggcccgacgg taatatccat cgcaccatcg gtccttgccc caatgcgcag cgaggtggtg
    44401 acgatatcgg ccatcgcttc acttaccggc cacggcgaca aactttgcga gtcgttaaag
    44461 cgcatcagcg cggaatcttt tttataggtc gaaagcagct gatcgtcggc gtccagctgg
    44521 gtctgaatct tttctttaag ttcggcactg cgtttggcat caatgcccgg gatgctggca
    44581 cgccagaagg tacccatggt tttgccttca agaacggtaa cttcagtagc gtgggttttg
    44641 gcgggttgtg gtttttgatc gcaaccaaca aagaagagcg cggcaaccag cagtgccacg
    44701 cgggtaaagc ttatttccat acgtgattat cctcatgcga acggtcgcaa gagtacacca
    44761 aaaaactgtg tttgtacgct gaaaacaatg aaaaaagggc ccgcaggccc tttgttcgat
    44821 atccatcgag attagaactg gtaaaccaga cccagagcta cgatgttatc agtgttgatg
    44881 ccagcgtcac gagtgaactg gttgtcgtcc agcaggttga ttttgtagtc aacgtaggtg
    44941 gacatgtttt tgttgaagta gtaggtcgca ccaacatcaa catatttcag gatatcttcg
    45001 tcgtcgtagt tacgaccatt gatgacaccc aggtttttac ctttagactg caggtaagcc
    45061 agggacggac gcagaccgaa gtcgaactgg tactgagcaa cagcttcgaa gttctgtgct
    45121 ttgttcgccc aacccaagtt gcctacgcga gttgcgttgt aggtctgggt gtactgagca
    45181 gctaggtaga tgttgttcgc gtcgtatttc agaccaccgg tgtaggtttc agcgcggtcg
    45241 ccattaccga tgtaacgctc atcataacgg ttcagaccga agttctggtc atcagtacgt
    45301 ttggagctgg aaactgcagc accgataccg aagccttcgt aatcataagt aatagaaccg
    45361 ccaacgccgt caccgttctg acgcagagct tcacgaccgt tgttggtcat gccttcacct
    45421 tccgggctac cgtttttgcc ctgatactga acagcgaagt tcagaccgtc aaccagacca
    45481 aagaagtcgg tgctacggta ggttgcgaag ccgttaccac gctgctgcat gaagttgtca
    45541 gaaccgtaag tgtcaccacc gaattctggc agtacgtcgg tccaggaagt tacgtcgtaa
    45601 actacgccgt agttacgacc gtagtcgaaa gaacctacat cctggaattt cagacctgcg
    45661 aatgccacac gggtccagga gttgttttcg ttttcagcgc tgttgccctg gatctgatat
    45721 tcccactggc cgtaaccggt cagctggtca gtaacctgag tttcaccttt gaagccaaga
    45781 cgcatgtagg tctggtcgcc atctacagac ttgtcgtcag agaaatagtg caggccgtct
    45841 actttaccgt acagatctaa tttgttgccg tctttgttgt aaacttcagc agcgtttgct
    45901 gcgcctgcta ccagcagagc tgggaccagg agggacagta ctttaacttt catgttatta
    45961 accctctgtt atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat
    46021 taatcagtcg gcaagtccat tctccccaaa aatgcagaat aatccaacac gaatatgata
    46081 ctaaaacttt taagatgttt catttatcgc tatagatgtt tcaaaatgta aatacaaggg
    46141 aactttttaa gattattgcg gaatggcgaa ataagcacct aacatcaagc aataataatt
    46201 caaggttaaa atcaataact tattcttaag tatttgacag cactgaatgt caaaacaaaa
    46261 ccttcactcg caactagaat aactcccgct atcatcatta actttattta ttaccgtcat
    46321 tcatttctga atgtctgttt acccctattt caaccggatg cctcgcattc ggttttttta
    46381 cccttcttta cacacttttc attattctgt gctaccacag aaaaactata acgcttgtta
    46441 actatttcac aaataattaa catcagcata atttccagca atctttgttt atttgcaatt
    46501 aatttcgttg ggctttttgt aggttatttg tacagcaaaa tggcgcttgt acatctattt
    46561 ccccccatac aggatgataa atatcacggg agaatagaga atcatcaatc aggtaagagt
    46621 ctggaatttc acactgtacc ctttatactg ccctatcact tcgcgaagtt ttaacaggtc
    46681 ataaacacga atgcgtcaga aagagacaac ggccacgacc cgcttttcac tcctaccggg
    46741 gagcattacc cgcttctttt tactgttgat cattgtgtta ctggtgacga tgggggtaat
    46801 ggtacaaagt gccgttaacg cttggctgaa agataaaagt taccagattg tcgacattac
    46861 ccacgcaatc caaaagcgcg tcgatacctg gcgttacgtg accaggcaga tctacgacaa
    46921 cattgccgcg acgacctccc cctcctccgg cgaaggttta caagagacgc gcctgaaaca
    46981 ggatgtctac tatctggaga aaccacgccg caaaacggaa gcgttaatct ttggctctca
    47041 cgacaactca acgcttgaga tgactcagcg gatgtccact tatctggata cattgtgggg
    47101 tgcagaaaat gtaccgtggt cgatgtatta cctgaatggt caggataaca gtctggtgct
    47161 gatctcaacc ctacccctta aagatctcac ctccggattt aaagaatcga ccgtcagtga
    47221 cattgttgat tcacgtcgtg cagaaatgtt acaacaggcc aacgccctcg atgaacgcga
    47281 aagtttttct aacatgcgcc gcctagcctg gcagaacggt cattacttta ccttgcgtac
    47341 cacgttcaac cagccgggac atctggcaac ggtcgtggct tttgatctgc cgattaatga
    47401 tttgatccca ccgggtatgc cgctggacag tttccgcctt gagccagacg cgacggcaac
    47461 gggaaacaat gataatgaga aagaagggac ggatagcgtc agtatccact ttaacagtac
    47521 gaagattgaa atctcctcgg cactcaactc taccgatatg cgcctggtct ggcaggttcc
    47581 ttatggcacc ttattgctgg atacgttgca aaacattctg ctgccactgc tgctgaacat
    47641 cggtttgctg gcgctggcgt tatttggcta taccacattc cgccatttct ccagccgcag
    47701 tacagaaagc gtccccagca cggcggtcaa taacgaattg cgcattttac gggcaatcaa
    47761 tgaagagata gtctcactgt tgccgctcgg cctgctggtt cacgatcagg aatcgaaccg
    47821 cactgtcata agtaacaaaa ttgccgatca tttgctgccg catctgaatc tgcaaaacat
    47881 caccaccatg gcggaacagc atcaggggat tattcaggcg acgatcaata acgagctgta
    47941 tgagatccgc atgttccgca gccaggtcgc gccgcgcaca caaattttca ttattcgcga
    48001 tcaggatcgc gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc tgtatgagaa
    48061 aaaccagcag gggcggatga cctttatgaa aaacattggc gatgcgctga aagaacccgc
    48121 acagtccctg gcggagagtg cggctaaact caacgccccg gaaagcaaac aactggcgaa
    48181 tcaggcagat gtgctggtgc ggctggtcga agaaatacag ttagcgaaca tgcttgcgga
    48241 tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg atgaagttgt
    48301 gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta acaatcatct
    48361 gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt tgctgctact
    48421 gatgcaatat gccgtgacct caacgcaatt gggaaaaatc acccttgagg ttgatcagga
    48481 tgagtcctct gaagaccgcc tgacattccg cattctggac acgggagaag gcgtaagtat
    48541 tcatgaaatg gataatttgc acttcccgtt tatcaaccag acccaaaacg atcgttatgg
    48601 aaaggcggac ccgctggcat tctggctgag cgatcaactg gcacgtaaac tgggcggtca
    48661 tttaaacatc aaaacgcggg atgggcttgg tacacgctac tctgtgcata tcaaaatgct
    48721 cgccgctgac ccggaagttg aagaggaaga agagcgttta ctggatgatg tctgcgtaat
    48781 ggtggatgtt acttcggcag aaattcggaa tattgtcact cgcctgttag aaaattgggg
    48841 tgcaacctgt atcacacccg atgaaagatt aattagtcaa gattatgata tctttttaac
    48901 ggataatccg tctaatctta ctgcctctgg cttgctttta agcgatgatg agtctggcgt
    48961 acgagaaatt gggcctggtc aattgtgcgt caacttcaat atgagcaacg ctatgcagga
    49021 agcggtctta caattaattg aagtgcaact ggcacaggaa gaggtgacag aatcgcctct
    49081 gggcggagat gaaaatgcgc aactccatgc cagcggctat tatgcgctct ttgtagacac
    49141 agtaccggat gatgttaaga ggctgtatac tgaagcagca accagtgact ttgctgcgtt
    49201 agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg tacccggcaa
    49261 gcagttatgt gaaacgctgg aacatctgat tcgtgagaag gatgttccag gaatagaaaa
    49321 atacatcagc gacattgaca gttatgtcaa gagcttgctg tagcaaggta gcctattaca
    49381 tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc ggtattcgca
    49441 aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac tctacagcac
    49501 tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc tccatgcctg
    49561 gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat ttcccaagcc
    49621 tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg gtattggatc
    49681 tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg aaagctctcg
    49741 ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg ttggaaaaaa
    49801 tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa gttctgcgcc
    49861 tgtttgcgga aggcttccta gtgaccgaga tcgctaaaaa gctgaaccgc agtattaaaa
    49921 ccatcagtag ccagaagaaa tctgcgatga tgaagctggg cgtcgagaac gatatcgccc
    49981 tgctgaatta tctctcttct gtgaccttaa gtccggcaga taaagactaa tcatctgtag
    50041 gccagataag acgcgttagt gtcttatctg gcatttgcac cgattgccgg atgcggcgta
    50101 aacgccttat ccggcctacg attcccatta tttcaacaga ttacattaaa gtaggccaga
    50161 taagacgcgt cagtgtctta tctggcattt gcactgaatg ccggatgcgg cgtaaacgcc
    50221 ttatccgtcc tacgaatccc gcgatttcct gaccctctcg gcatataccg tcagcgtctg
    50281 ttttatcaca tccagcgtta ccggcttcga gaggcagctg tccataccgg actccagaca
    50341 ccgctgcttc tcttcagcca acgcattagc agttactccg attaccggca acgtcagtcc
    50401 caactgacga atgcgttgcg tcaagcggta accatccata tttggcatgt tgacgtcgct
    50461 aagcacgata tcaatatgat tcttactaag tacattaagc gcatcgacgc catcattcgc
    50521 ggttttacat tgatagccca acgatcccaa ctgatctgcc agcagacgtc ggttaatcgg
    50581 atgatcatcc acgaccagaa tcatcatatc gtcgttatcg ctgaccgctt tgtccgtcga
    50641 cggcagagcg ttagcaggat cgtcgctctc catctcgatc aaataaatac gcgccaacaa
    50701 tgccggtagc tcatgcggag cagccacact gtgtacccac tcccctggcg ctttctccag
    50761 cggaatacca atatggcgac gacagaaggt cactaccgct ctgccctgcc attttttact
    50821 cactacctcg tcagtgatta acacatcttc gggagtcggt tcctgccctt cgtatgttgt
    50881 aacgacgatg ccgctgcgct gcaaactggt ttccaggaac tgacagagcg acgcattgcg
    50941 gaccgccagc cagcagcgtt taccactcaa cccttccacg ccttttttct gcgggtactg
    51001 agcgccgtac aacggaatac gcacggtaaa ctggctgccc attcccggtt ctgaatctac
    51061 cgagatatcg ccgtccatca tgctgatcag tttttcacaa atcgccagac ccagaccggt
    51121 cccctggaag tttcgctgta cgcccgttcc aacctggaag aagggatcaa acaagcgcac
    51181 cacttctttc gccggaatcc ccacgccggt atcgcgaaca cggatagaga gataatcgcc
    51241 atcagcgcga acatgcaaaa ctatacagcc ggtatcggtg aattttatgg cgttactcaa
    51301 caggttggag atgacctgct gtaaacgcat cgggtcgcca tttaaggcca ctggcacatc
    51361 cggttcaata aagcagtaca agcctaactg cttgcgtacc accagcggta aatagttggc
    51421 ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc ggttcgatct tcaactgttc
    51481 cgattcaatc ttcgagaaat cgagaatatc gctgataatt ttcaacaaca ggctggaaga
    51541 gttgttcatt accgtcacca gacgatcgac gcctttcggt aactctttgg tttgcaacag
    51601 atccaggtta ccgataatgc catacagcgg cgttcgcagc tcatgactga cggtggcaag
    51661 gaacatcgat tttgactggc tcgcctgttc cgctgcttgt gccatctcct gcaacgactc
    51721 ttccatcttc acgcgcgaag aaacatccac cagcacacaa atggccacgt tttcattacg
    51781 atagcgcgaa tggacgaagc tgatttgcag attggtattg ttgctggtca ggacatcaac
    51841 aaaattgacc tgctgcccac agataatttg cgtcagtcgt tggcggtcct catgcgtaag
    51901 catattgaga taggtatgcg ccagttcgtt acttaaaata ttgacgccat cagcggtacg
    51961 caaaatgcag atacccactg gcgcggaggc gacaatcttg cgattgaact gctcatgttc
    52021 ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt acatccgcgc
    52081 gagagtaaac aatgcagctc cggcaagcac attcagcaaa attgcgttaa ggatcaacat
    52141 gcgaatgcgt tccagcacct tatcaaccgg caccgaatac acgatgctta gcgatgaggg
    52201 tggcagattt ttcttcagca ccagctcccg gaacccttcc gtatagccaa accaggagcg
    52261 ttcctgcatc cagcgaggat cgcccttaat tttactttct ggtccggtaa gcgaaatcag
    52321 ggtatgacca ttttcatcaa gaatggtaac ccccatcggc aacgtacccg gtaagaaaaa
    52381 gttctccatc cggatggtct gctcgacacc caaaagcgcc tgcaaccggt tcgccagata
    52441 aactggcgtc aacgcgtaaa aatacccgac gcaagggcgc ggaccttcgc tgatccagta
    52501 gaggttactg ccgctatcat cttgtggtgc atttcgatat ttattgatgc gttcatgcaa
    52561 cgctttcaac gcggtatcgc gttccactgg catatcacgc agaccgaaat tggccatgca
    52621 gaggttatca ctgccgatta aaaatacccg gttgagatcg taagccgcag aaaaattatc
    52681 gcgccagtag cgcataaacc acgccaatga ctccagagaa cctcgccagg tgttactcat
    52741 tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg cctgcgtttc
    52801 tcgtccacgc ggggaaagca caccgttttc tgccgataag cgattttcgg cgatgtactt
    52861 aagctctttc atcacatcag aggtgcgttg aataaagcgc tgagcctgat cggaactcag
    52921 attaaattcc tgacgaattt ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac
    52981 ggatgaaaaa gcaatcaaca gccagagcac taacgccaat gctctgaaca tgtagcgcga
    53041 ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa ggggagaagc tccgcctcag
    53101 gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat gttctgtcac
    53161 cattgcaata atgcgtatcg cttagaaacc attgccttaa ccaggaaagc cggagtagat
    53221 ggacagttct cagtacgaaa aataagaaat gaccaatctg ccgacgcttg ttaatggtta
    53281 tatcctggcg tcggcagatc gcccagcagt cttatctgaa aaaagagaga gccaggttgt
    53341 gtgaagggat ggatcaccag tcaaaacttt tcaccagcgt cagctcgcca gcattacgca
    53401 tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg atcagcgtca
    53461 gttgggcggt cgtcaattcc gtttcgctgc gcccaccata gtagttgata tacacctgat
    53521 agcgcccgtg aactggtgct ggcatggcga aaatctcggg tccgtacccc gtcgtgacat
    53581 ccatatccag tgcaccactg tttttcagca cggtgttacc gtaccaggcg tgttcgccat
    53641 cgggcgtgac aacatggaga tcgagatcgg tattgtccgt atcccacgag agaaccagcc
    53701 gtaaacgtgc acgaatagtt cctgtacccg gcgttgagta aaactgcatt ttttgccggc
    53761 tttgcccatc cgggctgatg acctgcacgc tgttgctgcc ttcagtgaaa atataagggc
    53821 gtgcaaatga accatcggat tcaattctct gcggcatact ggcaccgttg accaccagcc
    53881 ggccctgctg aactttaccc gccgcagcat aattctttat tttgccgcgg atctgcgctg
    53941 agatattttg atcatctgcc atgttgacgg acgacgccgg gtaattgatc gattgtgaaa
    54001 aactggcatc ttcgccctct gccggatgcc agccagagag cggtgcgtca atctcgactt
    54061 cctgcacacc ttcactgtga gcgacaggcg aaagtgccac cagtaacaac ggaagaaaaa
    54121 tctttcgcat agcattagtc cattaataat tgtcgggtca gcgtttcgat atagttctca
    54181 tcaagcccgg tgggataacc atcaaaggcc aagtgcagat actcatgggt taaatccagc
    54241 cgatcctgaa gcgtaaagaa gttacgaata aacagccgtt tctgctgccg atcggtatag
    54301 gggaaacctg agactaaacg acagaccgca aacacgtctg gttcgttgta ccccgtctcg
    54361 ccttgtaata tacgccgcca ctgcggcatt tttttcgcca gccaggcttt cgctttgggt
    54421 aataactggc aggttgagcg tggagctccc cagcggctaa ggctgttgtc gggataagca
    54481 aacgcgagta tctggtcgta acgctccccc tgccccgcct gggccgtggc ttgccgccag
    54541 gaaagtgtcc cttcggtagc gcgactgcca tgataatgaa ccggatcgcc tgcatagatg
    54601 agctcctgcg tccaggcggt catcgttcgc gcccccgtgg tcgctggcga agcagaaaca
    54661 cgctgcgtgg cgctgctgtc ggggatggtc aggcaatcac cttcccgatt ggcgttttgc
    54721 tgtaaaaaag tacgtatcgc cacagtcatg gctttcgctg cctcaggtgg cgtacttttc
    54781 gcctcgcgat ccagaactct ggtaacgtac tcttcacgat cgagatgcga ctgcaatttc
    54841 agtttgccct tttcgctcag taaggtcgtc tcaccgtggc tgacaaacgt aatgtggtta
    54901 ccattagtaa acgtcacacg atagcgccca ttaagtacgc tgggttttac tgccgtcgta
    54961 ctcttttccg ctgtgatttt cttcagcgga tagcgggcaa acagctccac ctctacacat
    55021 tgcccactgg caacctgcgt cggcactggc aaaacccgat taagcaccgt cgcataacgg
    55081 gttagtaccg ttttgctggt gccactgcca gtaacccaca gcggtgtacc atcggttaac
    55141 cagccagcaa aaccaccctg acggatttcc tgtttatcat cggcaaacca gctccaggtt
    55201 ttcacccgta cccgactacc gagcatggat gcgacaccga ttttcgcctc gtccagcacc
    55261 acatccagta agacttcctg agctttgttt tgcgcaggta acgtcgctaa tgaatcaagc
    55321 agcgatttaa ccgttaccga ggtttctggc ttcagcgttg taagagacgc taaccacgca
    55381 ggtgcctgac gctgttgcca gtattgcccc cacacatctg cgccaatatg caaccgctgc
    55441 ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat cgcgcgttat gctctcccca
    55501 gcctgacaac aataaacctc ttccggggag tttccgcgac actggtaggg ctgctccggt
    55561 tgatgggtat cctccagcca ggcgtaaaca tacagtttcc acaaactccc taacggcgtg
    55621 gtgagcgagt caggtaatgc tgagactttg gtcacgcctg atgacgacag ctgatacagt
    55681 tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc aagcgttggc
    55741 agagtaacca acgccaatag ccagacgatt ctgcgccagt tcacttattt cacctgcatc
    55801 ccggtccatt cactccctgc cgcgacactt tgctgcgcag gtgcatagga acgcacataa
    55861 cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg gcggaaagtg
    55921 accgttcccg tcagttcttt caccggcacc atataggcca gttcgcccat ttcattacgc
    55981 gctttttcca gcaattgccc ctgctgtttc gcggcgttgg gtttattgac tgaaatgccc
    56041 catgttgtgc gctcaacgtc ggctcccggc gggagcggta cttccacctg accatagcgc
    56101 agaactgcat cctgctcgct ggtaagcgta atttcatcga gatacagcgc atcgctgtca
    56161 atctcattgc tggtcaccgg ctgcagagta aagctcatct cttcttcacc ggggataagc
    56221 cgatacaact ggcgttcaac ggtcaccgga atgttacttt gttgagccgt ttttgccggt
    56281 tcacgccagc ggacctgcac atttgcggcg ataattcgtc accaaaagag agaatgtccg
    56341 gcacacccta accaacccaa cgccagtctt cgccccctcc agttaactta tgtttagccc
    56401 atgcgcccgc aggcgcaggc aaaacaactg gaggcattgt cgccatatat ttcgccagcc
    56461 agttcatggc cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca ttaaaatagc
    56521 ggccgtctgg gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg
    56581 attgctgttt gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt
    56641 caactgcgct tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg
    56701 agattccggt gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa
    56761 atcttcatct ttagttccgc gccgggcgat agcttcatcc ccccctttca acaacgtttt
    56821 gcacggcaga ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat
    56881 gttatccgcc tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt
    56941 tacgccgagc gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc
    57001 gtcaccattg ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat
    57061 cagccgcaga cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg
    57121 atcggcaagc gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac
    57181 cccaccccac ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt
    57241 ttcactactt tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc
    57301 accgccgagc atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag
    57361 ttttgtgcta atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc
    57421 agattgctga atattctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt
    57481 ctggcgcatc tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg
    57541 actgaagata aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact
    57601 ccacttcatg tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc
    57661 gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa
    57721 cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc
    57781 cacctcttca cgccgtggac gttcaggcat ttttactcgc cgctcgctgc ggttagtcgc
    57841 gccaggcgca accggctcgc tagagagcgc ctggtcatag ctgataaacg acagactgga
    57901 gctggtacgc acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat
    57961 ttctggttgc agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac
    58021 aggtttacct ttcagcgacg aggttaa
//
LOCUS       DAJVRL010000013        55427 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVRL010000013 DAJVRL010000000
VERSION     DAJVRL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.013"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(110..1483)
                     /gene="argH"
                     /locus_tag="OQN02_000782"
     CDS             complement(110..1483)
                     /gene="argH"
                     /locus_tag="OQN02_000782"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1641691.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1544..2320)
                     /gene="argB"
                     /locus_tag="OQN02_000783"
     CDS             complement(1544..2320)
                     /gene="argB"
                     /locus_tag="OQN02_000783"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1641692.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2328..3332)
                     /gene="argC"
                     /locus_tag="OQN02_000784"
     CDS             complement(2328..3332)
                     /gene="argC"
                     /locus_tag="OQN02_000784"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1641693.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3486..4637
                     /gene="argE"
                     /locus_tag="OQN02_000785"
     CDS             3486..4637
                     /gene="argE"
                     /locus_tag="OQN02_000785"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1641694.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4989..7640
                     /gene="ppc"
                     /locus_tag="OQN02_000786"
     CDS             4989..7640
                     /gene="ppc"
                     /locus_tag="OQN02_000786"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1641695.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7823..9556
                     /locus_tag="OQN02_000787"
     CDS             7823..9556
                     /locus_tag="OQN02_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1641696.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9705..10556
                     /locus_tag="OQN02_000788"
     CDS             9705..10556
                     /locus_tag="OQN02_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1641697.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10543..10884)
                     /locus_tag="OQN02_000789"
     CDS             complement(10543..10884)
                     /locus_tag="OQN02_000789"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1641698.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10886..11764)
                     /locus_tag="OQN02_000790"
     CDS             complement(10886..11764)
                     /locus_tag="OQN02_000790"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1641699.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11730..14027)
                     /locus_tag="OQN02_000791"
     CDS             complement(11730..14027)
                     /locus_tag="OQN02_000791"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1641700.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14078..14398)
                     /locus_tag="OQN02_000792"
     CDS             complement(14078..14398)
                     /locus_tag="OQN02_000792"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1641701.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14413..15492)
                     /locus_tag="OQN02_000793"
     CDS             complement(14413..15492)
                     /locus_tag="OQN02_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1641702.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15801..16904
                     /locus_tag="OQN02_000794"
     CDS             15801..16904
                     /locus_tag="OQN02_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS1641703.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16905..18302
                     /gene="ptsP"
                     /locus_tag="OQN02_000795"
     CDS             16905..18302
                     /gene="ptsP"
                     /locus_tag="OQN02_000795"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1641704.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18314..18976
                     /gene="fsa"
                     /locus_tag="OQN02_000796"
                     /pseudo
     CDS             18314..18976
                     /gene="fsa"
                     /locus_tag="OQN02_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18987..20088
                     /gene="gldA"
                     /locus_tag="OQN02_000797"
                     /pseudo
     CDS             18987..20088
                     /gene="gldA"
                     /locus_tag="OQN02_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20363..20980
                     /locus_tag="OQN02_000798"
     CDS             20363..20980
                     /locus_tag="OQN02_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS1641705.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21007..21912)
                     /gene="yijE"
                     /locus_tag="OQN02_000799"
     CDS             complement(21007..21912)
                     /gene="yijE"
                     /locus_tag="OQN02_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1641706.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22006..24186)
                     /gene="katG"
                     /locus_tag="OQN02_000800"
     CDS             complement(22006..24186)
                     /gene="katG"
                     /locus_tag="OQN02_000800"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1641707.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24515..25405)
                     /gene="metF"
                     /locus_tag="OQN02_000801"
     CDS             complement(24515..25405)
                     /gene="metF"
                     /locus_tag="OQN02_000801"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1641708.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25754..28186)
                     /gene="metL"
                     /locus_tag="OQN02_000802"
     CDS             complement(25754..28186)
                     /gene="metL"
                     /locus_tag="OQN02_000802"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1641709.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28189..29349)
                     /gene="metB"
                     /locus_tag="OQN02_000803"
     CDS             complement(28189..29349)
                     /gene="metB"
                     /locus_tag="OQN02_000803"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1641710.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29626..29943
                     /gene="metJ"
                     /locus_tag="OQN02_000804"
     CDS             29626..29943
                     /gene="metJ"
                     /locus_tag="OQN02_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1641711.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30127..30735
                     /locus_tag="OQN02_000805"
     CDS             30127..30735
                     /locus_tag="OQN02_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1641712.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30796..31008)
                     /gene="rpmE"
                     /locus_tag="OQN02_000806"
     CDS             complement(30796..31008)
                     /gene="rpmE"
                     /locus_tag="OQN02_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1641713.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31211..33409
                     /gene="priA"
                     /locus_tag="OQN02_000807"
     CDS             31211..33409
                     /gene="priA"
                     /locus_tag="OQN02_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1641714.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33565..34590
                     /gene="cytR"
                     /locus_tag="OQN02_000808"
     CDS             33565..34590
                     /gene="cytR"
                     /locus_tag="OQN02_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1641715.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34682..35641
                     /gene="ftsN"
                     /locus_tag="OQN02_000809"
     CDS             34682..35641
                     /gene="ftsN"
                     /locus_tag="OQN02_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1641716.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35734..36264
                     /gene="hslV"
                     /locus_tag="OQN02_000810"
     CDS             35734..36264
                     /gene="hslV"
                     /locus_tag="OQN02_000810"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1641717.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36274..37605
                     /gene="hslU"
                     /locus_tag="OQN02_000811"
     CDS             36274..37605
                     /gene="hslU"
                     /locus_tag="OQN02_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1641718.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37672..38598
                     /gene="menA"
                     /locus_tag="OQN02_000812"
     CDS             37672..38598
                     /gene="menA"
                     /locus_tag="OQN02_000812"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1641719.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38691..39176
                     /gene="rraA"
                     /locus_tag="OQN02_000813"
     CDS             38691..39176
                     /gene="rraA"
                     /locus_tag="OQN02_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1641720.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39261..39506)
                     /gene="zapB"
                     /locus_tag="OQN02_000814"
     CDS             complement(39261..39506)
                     /gene="zapB"
                     /locus_tag="OQN02_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1641721.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39932..40777
                     /gene="glpF"
                     /locus_tag="OQN02_000815"
                     /pseudo
     CDS             39932..40777
                     /gene="glpF"
                     /locus_tag="OQN02_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40817..42307
                     /gene="glpK"
                     /locus_tag="OQN02_000816"
     CDS             40817..42307
                     /gene="glpK"
                     /locus_tag="OQN02_000816"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1641722.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42537..43547
                     /gene="glpX"
                     /locus_tag="OQN02_000817"
     CDS             42537..43547
                     /gene="glpX"
                     /locus_tag="OQN02_000817"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1641723.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43644..44390
                     /gene="fpr"
                     /locus_tag="OQN02_000818"
     CDS             43644..44390
                     /gene="fpr"
                     /locus_tag="OQN02_000818"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1641724.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44395..44823)
                     /gene="uspD"
                     /locus_tag="OQN02_000819"
     CDS             complement(44395..44823)
                     /gene="uspD"
                     /locus_tag="OQN02_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1641725.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44850..45149)
                     /locus_tag="OQN02_000820"
     CDS             complement(44850..45149)
                     /locus_tag="OQN02_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1641726.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45361..45801)
                     /locus_tag="OQN02_000821"
                     /pseudo
     CDS             complement(45361..45801)
                     /locus_tag="OQN02_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45902..46501
                     /locus_tag="OQN02_000822"
     CDS             45902..46501
                     /locus_tag="OQN02_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1641727.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46609..47376
                     /gene="tpiA"
                     /locus_tag="OQN02_000823"
     CDS             46609..47376
                     /gene="tpiA"
                     /locus_tag="OQN02_000823"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1641728.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47431..48186)
                     /gene="cdh"
                     /locus_tag="OQN02_000824"
     CDS             complement(47431..48186)
                     /gene="cdh"
                     /locus_tag="OQN02_000824"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1641729.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48293..49282)
                     /gene="sbp"
                     /locus_tag="OQN02_000825"
     CDS             complement(48293..49282)
                     /gene="sbp"
                     /locus_tag="OQN02_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1641730.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49602..50564)
                     /gene="pfkA"
                     /locus_tag="OQN02_000826"
     CDS             complement(49602..50564)
                     /gene="pfkA"
                     /locus_tag="OQN02_000826"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1641731.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50745..51647)
                     /gene="fieF"
                     /locus_tag="OQN02_000827"
     CDS             complement(50745..51647)
                     /gene="fieF"
                     /locus_tag="OQN02_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1641732.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51795..52295)
                     /gene="cpxP"
                     /locus_tag="OQN02_000828"
     CDS             complement(51795..52295)
                     /gene="cpxP"
                     /locus_tag="OQN02_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1641733.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52445..53143
                     /gene="cpxR"
                     /locus_tag="OQN02_000829"
     CDS             52445..53143
                     /gene="cpxR"
                     /locus_tag="OQN02_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1641734.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53140..54513
                     /gene="cpxA"
                     /locus_tag="OQN02_000830"
     CDS             53140..54513
                     /gene="cpxA"
                     /locus_tag="OQN02_000830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1641735.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54619..55293)
                     /gene="yiiM"
                     /locus_tag="OQN02_000831"
     CDS             complement(54619..55293)
                     /gene="yiiM"
                     /locus_tag="OQN02_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1641736.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13503 a        15020 c        14160 g        12744 t
ORIGIN      
        1 tgctgacaaa tttctgcctg atgcggcgta aacgccttat ccgcctacga agtgtcgcac
       61 tttgtaggcg tggtaaactc cccattttca ggggagcaat ataaacaaat tactctaacc
      121 gagcctgcgc aaaagcaatc gcctgcgcca cctgctgcgg tgagacgccg ccttttgccg
      181 cacgcttttc gaggcacgat tgtagcgaca gaatcggata gacatcttcg ccaatcacct
      241 gactgaattt ctgcaactca ctgagtggca gatcttccag cggtttgccc tgacgaatgg
      301 cttccaccac cgcttcaccc acaatatggt gcgcctcgcg gaaaggtacg cctttcgcca
      361 ccaggtaatc cgccagttca gtggcgttcg catacccctg ctgcgccgct tcctgacaac
      421 gcggacgttt cacctgaatg ccgtccagta ccagcgccgc catatgcagg cagtccagcc
      481 aggtatcgag cgcgtcgaac agaccttctt tgtcttcctg catatctttg ttgtaagcca
      541 gcggcaaacc tttcagcgtc atcatcatgc cggttaacgc accctgcacc cggccgcatt
      601 taccgcgaat cagctccagc gcatccgggt ttttcttctg cggcattaat gatgaaccgg
      661 aagtcacgcg gtccgaaagc tccacaaacc ccgcttcgcc ggtgttaaag aaaatcagat
      721 cttcagcaaa acgcgacaga tgcaccatgc cgatagcggc agcagaaagc agttccaaca
      781 catggtcacg gtcagaaacg ctgtccagac tgttacgggt cgccgaagca aagcccagcc
      841 agcctgctaa ctgttcacgg tcgatttcat aggccgttcc cgccagcgcg ccacagccta
      901 atgggctgac atccagacgc ttaagcgcat cctgcaaacg gctttcatca cgcgccagca
      961 tctcaacata ggccaggcac cagtgcgcga acgtcaccgg ctgggcgcgt tgcaggtgag
     1021 tgtaacctgg cattaccgcg tcctgattgt tttgtgcggt ttccaccagc gccgattgca
     1081 gctgccggtt agccgtcagt aactcgctaa cggtatcttt gcaccacagt ttcaggtcag
     1141 tcgctacctg atcattacgg ctacgcccgg tatgcagctt tttgcctaac tggcccactt
     1201 tgtcgatcag tttgccttcc acccagctat ggatatcttc ggcgtcgctt tcaaggattt
     1261 gttgtggcct ggcgcgaaca tcttccagca acacgttcag cgcctcttcc agttgcgcct
     1321 gctcttctgc agttaacacg ccgaccgtta ccaaggcttt ggaccaggcc acagagccaa
     1381 caatatcctg ctccgccaga cggtaatcaa agcgcagtga gtcgttgaat tgtttgaacc
     1441 gttgatctgc tgcctgggta aaacgcccgc cccaaagtgc cataactctg tttccttatt
     1501 ttgaaattca atgccggaaa gctgcgcctc cggctaacaa aacttaagct aaaatccgcg
     1561 tacccatcgg cataccgtta aacagtgccg gaagctgctc cgcataacgc caagaagcga
     1621 tatctaccgg acggcccagc gtgcgggccg catccagcgc cgcgttcact ttcactatca
     1681 tgccgtcagt aataatgccc tgctcaatca gttgttctgc tttcgcggcg gtcatttcgg
     1741 caatgcgttg ccctttgccg tcgagaatgc cgctgacgtc ggagagcaaa atcagatccg
     1801 cgcccagcgt tgccgccagc gccgttgccg cctggtcggc attgacgttc atcagttgcc
     1861 cttcgtctgt tacgccaatg gagctgacca ccggcagata accgttctcc agcaaggagt
     1921 tgataagctt aggcgaacct ggctgcgcca gtccaacatg acctaactct tcatcaagct
     1981 gggtcacttt gacgctgtcg ccgtcaccga gaaacaaacc tacggccgca atctgatgtt
     2041 tcttcgccca tgccaacagg gttttatttg ccgttcccgc cagtgctccg gtgataatgt
     2101 ctatctgatc agcaggcgtc acccgcaggc cgtttttctt tttcaccggc agattcagcc
     2161 ctttcatcag ctcatccacc acgcaaccgc cgccgtgcac aatcaccagc ggacgctgat
     2221 gtgactcacg ataattcacc aatgcgctaa acagacgttc cagcgcctct tcactatcca
     2281 gcagtacgcc gcccagtttg ataattaatg gattcatcat tgcaccctta aataagagac
     2341 tgcgtttccg catagccgaa acgaatattg gcgcactgta ccgcttgtgc cgccgcgcct
     2401 ttcagtaagt tgtcttcggt cgccacaata atcagatgct cgccctgaac ggcaaacccg
     2461 atatcgcaaa atggcagccc aacgacattt ttcagcgccg gaacgccttt gtcatacagc
     2521 cgcaccagcg gtttatgggc atacgcctgc tgtaacgctt gcgcgacttg cgcctgggtc
     2581 acacccgatt tcaggcggca ggtaatggtt tcgagaatgc cgcgcgggaa attgcccaga
     2641 tgtggggtga agataacgtc agcaccgagg tgtgtggcga tctctggttg atggcgatga
     2701 gtaaagacgc catacggttg caggctaact tcacaaaaac tatttgaaat cgctgcctta
     2761 cgtcctgcac cgctcacgcc gctggtggcg ttgatcaccg gccactgatt gaggtcaaga
     2821 agatcggcat caatcaacgg tttcagcgcc agctgtgccg ccgtcggata acagcctggc
     2881 accgcaatca aattcgcttc ttttaattta ttaccgcacc actccgccag accgtaggct
     2941 gcctgttcca gcagttccgg gtattgatgg gtaaagccgt aatatttttc atagaaggtg
     3001 gcgtcgttaa cacgaaacgc gccggaaagg tcgaacacca cgcagcccgc ttcaagaaac
     3061 tgcggcgcta aatcgtggct aacttcatgg gcggtggcga gaaacactac gtccacccct
     3121 gggctaaact cgctgatatc cgacatcggc tgcaacggca gctcaacgat gccttttagc
     3181 tgcggatgca aatcggagat taactttccc gcatcattgc tttgcgctga aacagtcaaa
     3241 gcggttatgt tcatatgcgg atggcgattt acataggtca ctagctctgc gccagcgtag
     3301 ccgctggcac ccacaatcag cgtattcaac atcggggcta ttcaccttct tatgtctggt
     3361 tgccaggtta aacgtaaaac attcacctta cggctggtgg gttttattac gctcaacgtt
     3421 agtgtatttt tattcataaa tactgcatga atattgatac tatcatgacc agaggtgtgt
     3481 caacaatgaa aaacaaatta ccgccattta tcgagattta ccgcgctctg attgccacac
     3541 cttcaataag cgctacggaa gaggcactcg atcaaagcaa tgcagattta atcactctgc
     3601 tggcggactg gtttaaagat ttgggcttca atgtggaagt gcagcctgtt ccaggaactc
     3661 gcaacaaatt taatatgctg gccagttacg gacagggggc tggcggcttg ttgctggcgg
     3721 ggcataccga tacggtgcca tttgatgacg gtcgctggac gcgcgatccg tttacactga
     3781 cggagcatga cggcaagctt tacggcttag gcaccgccga catgaaaggc ttttttgcgt
     3841 ttatccttga tgcgctacgc gatgtcgacg tcacgaaact gaaaaaaccg ctctacattc
     3901 tggcgactgc tgatgaagaa accagtatgg ccggagcgcg ttattttgcc gaaactaccg
     3961 ccctgcgccc ggattgcgcc atcattggcg aaccgacgtc actacaaccg gtacgcgcac
     4021 ataaaggtca tatctctaac gccatccgta ttcagggcca gtcggggcac tccagcgatc
     4081 cagcacgcgg aattaacgct atcgaactaa tgcacgacgc catcgggcat attttgcaat
     4141 tgcgcgataa cctgaaagaa cgttatcact acgaagcatt taccgtgcca taccctacgc
     4201 tcaacctcgg gcatattcac ggtggcgacg cttctaaccg tatttgcgct tgctgtgagt
     4261 tgcatatgga tattcgtccg cagcctggca tgacactcaa tgaacttaat ggtttgctca
     4321 acgatgcatt ggctccggtg agcgaacgct ggccgggtcg tctgacggtc gacgagctgc
     4381 atccgccgat ccctggctat gaatgcccac cgaatcatca actggttgaa gtggttgaga
     4441 aattgctcgg agcaaaaacc gaagtggtga attactgtac cgaagcgccg tttattcaaa
     4501 cgttatgccc gacgctggtg ctcggtccgg gttccatcaa tcaagctcat cagcctgatg
     4561 aatatctgga gacgcggttt attaaaccca cccgtgagtt gattatccag gtaattcacc
     4621 atttttgctg gcattaaaac gtaggccgga taaggcgctc gcgccgcatc cggcaccgtt
     4681 gccaaactcc ggtgccgcaa taatgtcgga tgcgatgctt gcgcatctta tccgacctac
     4741 acctttggtg ttatgtatgg cgatttttta acatttccat aagttacgct tatttaaagc
     4801 gtcgtgaatt taatggcgta aattcctgct atttattcgt ttgctgaagc gatttcgcag
     4861 catttgacgt caccgctttt acgtggcttt ataaaagacg acgaaaagca aagcccgagc
     4921 atattcgcgc caatgcgacg tgaaggatac agggctatca aacgataaga tggggtgtct
     4981 ggggtaatat gaacgaacaa tattccgcat tgcgtagtaa tgtcagtatg ctcggcaaag
     5041 tgctgggaga aaccatcaag gatgcgttgg gagaacacat tcttgaacgc gtagaaacta
     5101 tccgtaagtt gtcgaaatct tcacgcgctg gcaatgatgc taaccgccag gagttgctca
     5161 ccaccttaca aaatttgtcg aacgacgagc tgctgcccgt tgcgcgtgcg tttagtcagt
     5221 tcctgaacct ggccaacacc gccgagcaat accacagcat ttcgccgaaa ggcgaagctg
     5281 ccagcaaccc ggaagtgatc gcccgcaccc tgcgtaaact gaaaaaccag ccggaactga
     5341 gcgaagacac catcaaaaaa gcagtggaat cgctgtcgct ggaactggtc ctcacggctc
     5401 acccaaccga aattacccgt cgtacactga tccacaaaat ggtggaagtg aacgcctgtt
     5461 taaaacagct cgataacaaa gatatcgctg actacgaacg caaccagctg atgcgtcgcc
     5521 tgcgccagtt gatcgcccag tcatggcata ccgatgaaat ccgtaagctg cgtccaagcc
     5581 cggtagatga agccaaatgg ggctttgccg tagtagaaaa cagcctgtgg caaggcgtac
     5641 cgaattacct gcgcgaactg aacgaacaac tggaagagaa tctcggctac aaactgcccg
     5701 tcgaatttgt tccggtacgt tttacctcgt ggatgggcgg tgaccgcgac ggcaacccga
     5761 acgtcactgc cgatatcacc cgccacgtcc tgctgctcag ccgctggaaa gccaccgatc
     5821 tgttcctgaa agatattcag gttctggttt ctgaactgtc gatggttgaa gcgacccctg
     5881 aactgctggc gctggttggc gaagaaggtg ccgcagaacc gtatcgctat ctgatgaaaa
     5941 acctgcgttc tcgcctgatg gcgacacagg catggctgga agcgcgcctg aaaggcgaag
     6001 aactgccaaa accagaaggg ctgctgacac aaaacgaaga actgtgggaa ccgctctacg
     6061 cttgctacca gtcacttcag gcgtgtggca tgggtattat cgccaacggc gatctgctcg
     6121 acaccctgcg ccgcgtgaaa tgtttcggcg taccgctggt ccgtattgat atccgtcagg
     6181 agagcacccg ccataccgaa gcgctgggcg agctgacccg ctacctcggt atcggcgact
     6241 acgaaagctg gtcagaggcc gacaaacagg cgttcctgat ccgcgaactg aactccaaac
     6301 gtccgcttct gccgcgcaac tggcaaccaa gcgccgaaac gcgcgaagtg ctcgatacct
     6361 gccaggtgat tgccgaagcg ccgcaaggct ccattgccgc ctacgtgatc tcgatggcga
     6421 aaacgccgtc cgacgtactg gctgtccacc tgctgctgaa agaagcgggt atcgggtttg
     6481 cgatgccggt tgctccgctg tttgaaaccc tcgatgacct gaacaacgcc aacgatgtca
     6541 tgacccagct gctcaatatc gactggtatc gcggcctgat tcagggcaaa cagatggtga
     6601 tgattggcta ttccgactca gcaaaagatg cgggcgtgat ggcagcttcc tgggcgcaat
     6661 atcaggcaca ggatgcatta atcaaaacct gcgaaaaagc gggtattgag ctgacgttgt
     6721 tccacggtcg cggcggttcc attggtcgcg gcggcgcacc tgcccatgcg gcgctgctgt
     6781 cacaaccgcc aggaagcctg aaaggtggcc tgcgcgtgac tgaacagggc gagatgatcc
     6841 gctttaaata tggtctgcca gaaatcaccg tcagcagcct gtcgctttac accggggcga
     6901 ttctggaagc caacctgctg ccaccacctg agccgaaaga gagctggcgt cgcattatgg
     6961 atgaactgtc agtcatctcc tgcgatctct accgtggcta cgtacgtgaa aacaaagatt
     7021 ttgtgcctta cttccgctcc gctacgccgg aacaagaatt gggcaaactg ccgttgggtt
     7081 cacgtccggc gaaacgtcgc ccaaccggcg gcgtcgagtc gctgcgcgcc ataccgtgga
     7141 tttttgcctg gacgcaaaac cgcctgatgc tccccgcctg gctgggtgca ggtacggcgc
     7201 tgcaaaaagt ggtcgaagat ggcaaacaga gcgagctgga agccatgtgc cgcgattggc
     7261 cattcttctc gacgcgtctc ggcatgctgg agatggtctt cgccaaagca gacctgtggt
     7321 tggcggaata ctatgatcaa cgtctggtag acaaagcact gtggccgtta ggtaaagagt
     7381 tacgcaacct gcaagaagaa gacatcaaag tggtgctggc gattgccaac gattcccatc
     7441 tgatggccga tctgccgtgg attgcagagt ctattcagct acggaatatt tacaccgacc
     7501 cgctgaacgt attgcaggcc gagttgctgc accgctcccg ccaggcagaa aaagaaggcc
     7561 aggaaccgga tcctcgcgtc gaacaagcgt taatggtcac tattgccggg attgcggcag
     7621 gtatgcgtaa taccggctaa tcttcctctt ctgcaaaccc tcgtgctttt gcgcgagggt
     7681 tttctgaaat acttctgttc taacaccctc gttttcaata tatttctgtc tgcattttat
     7741 tcaaattctg aatatacctt cagatatcct taaggaattg tcgttacatt cggcgatatt
     7801 tttttcaaga caggttctta ctatgcattc cacagaagtc caggctaaac ccctttctag
     7861 ctggaaagcc ctgggttggg cactgctcta cttttggttt ttctctactc tgctacaggc
     7921 cattatttac atcagtggtt atagtggcac caacggcatt cgcgactcgc tgttattcag
     7981 ctcgctgtgg ttgatcccgg tattcctctt tccgaagcgg attaaaatta ttgccgcagt
     8041 gatcggcgtg gtgctatgga cggcctctct ggcggcgctg tgctactacg tcatctacgg
     8101 gcaggagttc tcgcagagcg ttctgtttgt gatgttcgaa accaacacca acgaagccag
     8161 cgagtattta agccagtatt tcagcctgaa aattgtgctt atcgcgctgg cctatacggc
     8221 ggtggcagtt ctgctgtgga cacgcctgcg cccggtctat attccaaagc cgtggcgtta
     8281 tgttgtctct tttgccctgc tttatggctt gattctgcat ccgatcgcca tgaatacgtt
     8341 tatcaaaaac aagccgtttg agaaaacgtt ggataaccta gcctcgcgta tggagcctgc
     8401 cgcaccgtgg caattcctga ccggctatta tcagtatcgt cagcaactaa actcgctaac
     8461 aaagttactg aatgaaaata atgccttgcc gccactggct aatttcaaag atgaatcggg
     8521 taacgaaccg cgcactttag tgctggtgat tggcgagtcg acccagcgcg gacgcatgag
     8581 tctgtacggt tatccgcgtg aaaccacgcc ggagctggat gcgctgcata aaaccgatcc
     8641 gaatctgacc gtgtttaata acgtagttac gtctcgtccg tacaccattg aaatcctgca
     8701 acaggcgctg acctttgcca atgaaaagaa cccggatctg tatctgacgc agccgtcgct
     8761 gatgaacatg atgaaacagg cgggttataa aaccttctgg atcaccaacc agcagacgat
     8821 gaccgcccgc aataccatgc tgacggtatt ttcgcgccag accgacaagc agtactacat
     8881 gaaccagcaa cgtacgcaga gtgcgcgtga atacgacacc aacgtgctga agccgttcca
     8941 ggatgtgctg aatgaccctg cgccgaagaa actgatcatc gttcatctgc tgggtacgca
     9001 tatcaaatac aaataccgct acccggaaaa tcagggcaag tttgatggca ataccgatca
     9061 tgttccgcca ggattaagcg cagaagagct ggaatcatat aacgattatg acaacgctaa
     9121 cctgtataac gatcatgtgg ttgccagcct gattaaagac tttaaagcgg cagacccgaa
     9181 cggattcctg gtttacttct ctgaccacgg tgaagaggtt tacgacacgc cgccgcacaa
     9241 aactcagggg cgtaacgaag acaacccgac gcgccacatg tacaccattc cgttcctgct
     9301 gtggacgtcg gaaaaatggc aagcgactca tccccgtgat ttctcacagg atgtcgatcg
     9361 taaatacagc ctggcggaac tgatccacac ctggtcagat ttggcgggct tatcttacga
     9421 cggttacgat ccaacccgtt cagtggtgaa tccgcagttc aaagaaacta cccgctggat
     9481 tggtaacccg tacaagaaaa acgcgctgat cgattacgac actctgccgt atggcgatca
     9541 ggtgggtaat cagtagatga cctttgtatc gcgtctggcg ctatttgccg gatgcgatac
     9601 tgccgcaatg tttacatcga actcccctca ggccacactt ttcaggtggc cttttttaat
     9661 aatgccagtt actgttttcc cgtctacata cctaagggaa taacatgtat cacgacgtca
     9721 gctatctgct ctcccgcctg atcaacggcc cgctgtccct gcgccagatt tactttgcca
     9781 gcagtaacgg cccggtgccc gatcttgcgt atcaggtcga ttttccacgg ctggaaattg
     9841 tgctggaagg tgagtttgtt gataccggcg ctggagcaac gttagttccc ggcgatgtgc
     9901 tgtacgttgc cgctggtggc tggaatttcc cacaatggaa aacacccgct accaccttta
     9961 gcgtgctgtt tggcaaacag caactcggct tcagcgtcgt gcaatgggat ggcaaacaat
    10021 atcaaaatct ggcgaagcaa cacgtcgccc gacgcggccc acgcataggt tcttttctgc
    10081 tacaaacgct caatgaaatg caaatgcagc cgcaggagca gcaaacggca aggcttatcg
    10141 tagccagcct gcttagccac tgccgcgatt tgctcggcag ccaaatccag accgcctcac
    10201 gcagccaggc actatttgaa gctattcgcg attatatcga cgaacgctat gcctccgcgc
    10261 ttacccgcga atctgtcgca caggcattct atatttcacc aaactatctc tcgcacctgt
    10321 ttcaaaaaac gggggccatt ggttttaacg aatacctgaa tcacacgcga ctggagcacg
    10381 ctaagacgtt attgaaaggc tatgacctga aggtaaaaga agtggcgcac gcctgcggct
    10441 ttgtcgacag caactacttc tgccgtctgt ttcgtaaaaa caccgaacac tcgccgtcgg
    10501 aatatcgccg gcagtaccac agccaactga cagaaaaacc gactactcca gaataagatg
    10561 ggtttgctgc ggcgcagaaa gcactttgcg caccgcgctc attacccgct gcggctcacg
    10621 caggaatgcg tagatgctgc attgcacata gcggcaatgt tcaaatcgct cggcaccggc
    10681 aagctcgata tccgtaatca acagagcaac atccgcccga cggatatcct cgtccgctaa
    10741 acgattctcc gttcccagcg cgccctgagt ttcaatgctg acgccccact tctctaactg
    10801 gcacaacttt tccagccgtt ccgccgccat ataagtatgc gccacgccgc ttacgcaggc
    10861 ggttactgcc accaggtatg ccattttaac ctcccacggt aacctgaaat ccggcccgtt
    10921 ctgccatttc gcgcatcgtt gtcacatcgg ctgacgacgg cgcaggcacc tctttcatcg
    10981 accatgtttt ccccagcagg cggtatttcg gttcgccgta ctgatgaaac ggtaacagat
    11041 ggatctgcct gattttcagc gggatcagta catccagcgc ctgctgcata ttctcccggc
    11101 tgagcgtgaa accagggatc aacggtaaac gcgggatcac gttgacaccc tcactcacca
    11161 gcaaacgcag attctccagc acgcgtggca ggttcatctt caccacatcc cgcgcctgag
    11221 tcgcgtccat aatttttaaa tcgaacagca cttcatcgca caatttcgcc agcggtaaca
    11281 gcttgcttgc tggcgcgtct ccggcagttt caatggcgca tgagacaccc cacagccgca
    11341 gtcgctgtaa aaaacgggta gcaaactccg cctgcattaa cacttcgccg ccagaaagcg
    11401 tgacgccgcc gccggacgtg cgaaagaaaa tgtcatcttt catcacttcc cgttccagag
    11461 cgtcaaggct gatatcgcga ccaatccgtt caaacgcccc ggaggggcat tcatccgcat
    11521 cacgcaaaca tttcgcacag tgcagacatt tcgcctctct gcgtaccgtc tggattttgc
    11581 cggagatcga ctccggatta gcacaccacg ggcaaagatg cggacagcct ttaaaaaaga
    11641 ccaccgtacg aatgccctcg ccgtcattca gtgaataacg ctgaatgttg aaaatgcgcg
    11701 ccacatcatc gcggcgcgtt tccacaacgt tacagctgat gcgctgtccg gcggatgatg
    11761 tcatcctgga tctccttcga cagttcgaca aagaaggcgc tgtatccggc aacgcgcacc
    11821 accagcccgg cataatcttg tgggcgctgt tgcgcttccc gcaacgtgtc ggcgttcacc
    11881 acgttaaact gaatatgctg caacttaagc tgggtaaaca cccgtaagaa gtcggccagt
    11941 ttgcgtaatc ccgcttcacc ttccagggtc gccggagtga atttcacgtt cagcaacgta
    12001 ccgttagaca gcagcgtatt atcgagctta ctgactgact tcagtaccgc cgttggccct
    12061 tgtgcgtcct gacccagcat aggtgacaag ccgccgtctg ccagttgttc cccggcaaaa
    12121 cgaccgtctg gcgtcgcgcc aaccaccgat cccaacggaa cgtgagcaga aacggtatac
    12181 gatcccggcg tgaagtaacc gccgcgcggg ttctggtatt tttccacttc tttgcagtag
    12241 tggcgcaaca gttcggcgct aatgttatcc acctcgtcga tatcgttacc gtatttctca
    12301 aagcggttaa ttaagcgagc gcggactttt tcgccttctg gcgttgcaaa gttggctttt
    12361 aataccgaca gcaattcgtc aaaacttaaa cgctgttgct caaaaaccat ccctttcaac
    12421 gcatggagag aatcgctcag gttggcgata ccaattccct gtacgccgga gaagttataa
    12481 cgcgcgccgc catcggtaat atcgcgacct ttttccagac aatcgctgat aaaagacgag
    12541 agcagcggta caggtgccca gtcgcgatgg ccgatatcac aaatattgct gccctcaacc
    12601 atcagggtga tgtagtggct gatcttggcg cggatctgct ccagtaaacc ttcataagtc
    12661 agcgcagcat tgccttcatt ttcatgcagg cagatttcca tcactttcag cagattaaac
    12721 atcgcgatgt catgcaagcc gtaggttctg ccgggaatag ataattccac acagcccact
    12781 acggaatagt cgcgcgcatc ttccagcgaa acgccacggt tgaggaacgc tggcaccacc
    12841 acttcatcgt taaagatttg cggaataccg gtgccgaggc gaatggtttc ggcggttttc
    12901 atcaggaacg gcgtgtcgat caaggcgtta gtgcgcacgc cgaggttcgg ttgcggtaat
    12961 tgcacgcttt gataggtgtc aaggcaaagg aacgaaagca cgttcaccgc gctacgtccg
    13021 ttctcggtta acccgccgag cagtgcggta tagccggtcg ggaaaccggc gaaataacgc
    13081 gcgctactgg tggagcgcaa cagcacgatg tcgttgcatt tcacccataa agattcgagc
    13141 agttctttca ggaacgccgg atcttcgccc tgggttaaag atgcctgata gaacggcaac
    13201 atatactggt cgaagcgccc taaagacagc gaactggcgt tggattcgta ttgcagaatg
    13261 atgttcatgt accagaataa ctggcacgcc tgccagaacg tctgcggctt atgttgcgcg
    13321 ttatggcggg agatctccgc aatagtcagc agctcttcgc gacgctgggc atctgtgcag
    13381 tttgccgcca tcgtttccgc cagttcggcg taacgcaaaa tgtgtttctg cgaggcttcc
    13441 agcagtaaca acgcggcctg ataaaagtga ttctccggct gttgctgaca atgttgctgc
    13501 atctgtgcca ccagctcacc cagaccgtga ttcaacaggc gtgggtaatc aataataatg
    13561 tgcccctggc ctttatccgt ctggttgatg ctgaaaatct gcgtgttggt cgcggctttt
    13621 acttcatctg tcatctgccc gttgatgaaa tctttcatcg aacgtttttc ccagtacggg
    13681 aacaactctt cacggtagat acgtttatct tcttcgctga tagcaaagcg gtcctgcgga
    13741 cgcgtcggga attgatccag ctctttcagc agccagtaag ggtccatttc cggcgacata
    13801 atcccggcgc gcggttttac ggtgcggtta ccggcaatca gttcttcatc acgaatcgaa
    13861 atttcaacat gttcaaggat atacgctgtt gctttcgccc ggcgcaatat caccggttcg
    13921 ccttcggttt gccgatggct ggcggtataa agcagcgccc gctccagcga gatttcacgg
    13981 gtattggcaa acagtgcagt tttgaggcga gagatacgat tcgtcatgag gaactcctgt
    14041 gggaaagcga acccgccctt gcgagcggga gaagaactta agcggtttgc gccaggtgag
    14101 cttcaatttt gctcataatg gcgtcagcac gttttactgc atcgctgatg ttgacgcgca
    14161 cgatggtctt gccagcaaaa cgctcttcaa acttgatgcc gatatctttg gtcagaatca
    14221 ccatatcagc gctcgccacg tcttccgcag tcagttcatt ttccagacca attgacccct
    14281 gggtttctac tttcacttcc cagcctttcg ctttcgcggc actttccagc gcctctgctg
    14341 ccatataggt atgggcaaca cctgaaggac atgcggttac tgcaataatt ttcgtcatgg
    14401 ctcgttcctc aattaattaa tttcaaaatc caaatccagg tcgtcttctt tttcatcagt
    14461 cgaagaccca tttttacgcg ccagactttt cagcacgttc acacaaacag ccgtcaccac
    14521 tgctccgaca gccactgctg cgatataacc caacttacct tcaaccaccg gcagcacaat
    14581 cagaccaccc cagcctgcat agcactgcgc accgaccagc gccgcagtta ctgcaccaca
    14641 aactgaaccg accataatgg aaggaatgac acgcagcgga tcggcagcgg cgaaaggaat
    14701 cgccccttca gtaacgccaa cacagcccat caccagtgcc gctttgccgg tttcgcgctc
    14761 ttctgcggag aaatttttac gaccaatcag cgtcgccagc cccatcccca gcggcgggat
    14821 acaaataccc acggcagcaa tggccaccac ggtataaaca ccctgagcaa cgcaaatcag
    14881 catgaaggca taggccactt tgttaaccgg accgcccata tcgaacgcca gcatcagccc
    14941 catgatcacc gccagcataa caatgctgcc ctgctgcatc ccctgaagcc actgagtcag
    15001 gctgttggtc aacgccccta caggctcgcc caagccccac atcatgatgc ctgcggtaat
    15061 cagtgtgccg atgataggaa taatgaagat gggcatcacc gagcgcagaa ctttatgcac
    15121 cggaattttc ttcaggtaat gcaccacgat gccgccgata atcccggcga tcagcgcacc
    15181 aaagaagccc gcaccaaagc tgttaccaac ccaggcaccg atagcgcacg gagccagcgc
    15241 agaacgctct gcaatggagt aaccaatgta agcagcgagg aaaggcacca tcagcgtcaa
    15301 acctgcaacg ccgatatcaa acagtttttt caaattcgga tcggctacgg catccggcac
    15361 tgcgcctttg ccatacaaca tgacggaaac cgccagcaaa ataccgcccg ataccacgaa
    15421 gggaatcatg tgtgaaacgc ccgtcattaa atgctgacgg gtatttttca ggatctgcac
    15481 caactcattc ataagctgct cccgggcttg caaaagccca tgtccatatt tttgatggag
    15541 gaaacaatag gcaatcgcag gcggagcaaa cagtggataa aaaggccatt tactggactt
    15601 ttgtaatatc agtacaaaaa tgcgatccgc ctcataactt gcgataatcc ggaagcatgc
    15661 cgcaaaacgg cgtcttgtga gacgcgtcgc aaattgacgg ttacattaca tttgtccagc
    15721 ttttggcaga tttgtcacat ggcaggccgt taccgtgcgc tcttatcctg taaacagaaa
    15781 accgtaccgg gagaaaggca atggccctga ttgtggaatt tatttgtgag ctacctaacg
    15841 gcgtacatgc gcgtccggca agccacgttg aaacgctgtg taatactttt tcatctcaaa
    15901 ttgagtggca taacctgcgt actgaccgta aaggcaacgc caaaagcgct ctggcgctga
    15961 ttggcaccga tacgctggtg ggtgataact gccagctact gatttccggg gccgacgaac
    16021 aggaagggca ccagcgttta agccaatggc tgcgcgatga attcccccac tgcgatgcgc
    16081 cgctggcgga agttaaatct gacgaactgg aaccactgcc ggtttcactg accaatctga
    16141 atccgcaaat tatccgcgcc cgcaccgtgt gcagcggtag tgcaggcggc attctgacgc
    16201 cgatctcttc tttagatctc aatgcgctga gtaatcttcc cgcagccaaa gacgttgacg
    16261 ccgagcaatc cgcactggaa aacggcctga cgctggtact gaaaaacatt gagtttcgtc
    16321 tgctggatag cgacggtgct accagcgcga ttctggaagc gcaccgatcc ctggctggcg
    16381 atacttccct gcacgaacat ttactggcag gcgtcagcgc cggattaagc tgtgccgaag
    16441 cgattgtcgc cagtgcgaat cacttttgcg aagagtttgc ccgttccagc agcagatacc
    16501 tgcaagaacg tgccctggac gtacgcgacg tctgcttcca gttactccag caaatctacg
    16561 gtgagcaacg cttcccggca ccgggcaaac tgacgcagtc cgccatttgt atggctgatg
    16621 aactgacccc cagccagttc ctcgaactgg ataaaaatca cctcaaagga ttgttactga
    16681 aaagcggcgg caccacctca catacggtga tccttgcccg ttcgttcaac attccgacgc
    16741 tggttggtgt ggatattgat gccctcactc cgtggcagca tcaaacgatt tatatcgacg
    16801 gcaacgccgg ggcgattgtg gttgagccag gagaagccgt agctcgttat tatcagcaag
    16861 aagcccgcgt acaggacgcc ctgcgtgagc aacagcgtgt ctagctgacc caacaagccc
    16921 gtaccgctga cggtatccgc attgaaattg ccgctaacat cgctcactcc gtggaagcgc
    16981 aggccgcatt cggcaatggt gcggaaggcg ttggtttgtt ccgcactgaa atgctctata
    17041 tggatcgcac cagtgcaccg ggcgaaagcg agctgtacaa cattttttgt caggcgctgg
    17101 agtctgccaa cggacgcagc attattgtgc gcactatgga cattggcggc gacaaacccg
    17161 ttgattatct aaacattccc gcagaggcaa acccgttcct cggttatcgc gccgtgcgta
    17221 tttatgaaga atacgcgtcg ttatttacca cacaactacg ctcgatcctg cgcgcctccg
    17281 ctcacggcag cctgaaaatc atgatcccga tgatctcctc aatggaagag atcttatggg
    17341 tgaaagaaaa actggcagaa gccaaacagc aactacgtaa cgaacacatt ccgtttgatg
    17401 agaagatcca gctcggcatt atgctggaag taccgtcggt gatgttcatc atcgatcaat
    17461 gctgcgaaga gattgatttc tttagtattg gtagtaatga cctgacgcaa tatctgctgg
    17521 cagtggatcg cgataacgct aaggttactc gtcactacaa cagcctgaat ccggcattct
    17581 tgcgggcgct cgattacgac gtgcaggcgg tgcatcgcca gggtaaatgg attggtctgt
    17641 gcggtgagct gggagcgaaa ggttccgtgc tgccgttgct ggtcggctta gggctggatg
    17701 aactcagcat gagcgcacca tcaattccgg cggcgaaagc gcggatggcg caacttgata
    17761 gccgtgagtg ccgcaagttg ctcaaccagg caatggcctg ccgtacttcg ctggaagtgg
    17821 aacacctgct ggcgcaattc cgcatgaccc aacaggacgc accgctggtc accgccgagt
    17881 gcatcacgct ggaaagtgac tggcgcagca aagaagaagt gctcaaaggc atgaccgata
    17941 acctgctgct ggcgggccgc tgccgctatc cgcgtaaact ggaagccgac ttatgggcgc
    18001 gcgaggccgt tttctctacc ggtctgggct ttagttttgc cattccacac agcaaatcag
    18061 aacacattga gcaatccacc atcagcgtgg cgcgtctgca agcgccggtg cgctggggcg
    18121 atgatgaagc gcaattcatc attatgttaa ccctgaacaa acacgctgcg ggcgatcagc
    18181 acatgcgcat tttctcgcgc ctcgctcgcc gcatcatgca cgaagaattc cgtaacgcgc
    18241 tggttaacgc cgcctctgcc gacgctatcg ccagcctgct gcaacatgaa ctggaactgt
    18301 aaaaggaaac atcatggaac tgtatctgga caccgctaac gtcgcagaag tcgaacgtct
    18361 ggcacgcata ttcccgattg ccggggtgac aactaacccg agcattatcg ctgccagcaa
    18421 ggagtccatc tgggaagtgc tgccgcgtct gcaaaaagcg attggtgatg aaggcattct
    18481 gtttgctcag accatgagcc gcgacgcgca ggggatggtg gaagaagcga agcgcctgcg
    18541 cgacgctatt ccgggtattg tggtgaaaat cccggtgact tccgaaggtc tggcagcaat
    18601 taaaatactg aaaaaatagg gtattactac acttggcacc gctgtatata gcgccgcaga
    18661 agggttatta gccgcactgg caggggcaaa atacgttgct ccgtatgtta accgcgtaga
    18721 tgcccagggc ggggacggca ttcgtacggt tcaggagctg caaacgctgt tagaaatgca
    18781 cgcgccagaa agcatggtgc tgacagccag ctttaaaacg ccgcgtcagg cgctggactg
    18841 tttactggca ggatgtgaat ccatcaccct gcccttagat gtagcgcaac aaatgctcaa
    18901 cacccctgcg gtagagtcag ctatagagaa gttcgaacac gactggaatg ccgcatttgg
    18961 cactactcat ctctaaagga gcaattatgg accgcattat tcaatcaccg ggtaaataca
    19021 tccagggcgc tgatgtgatt aatcgtctgg gcgaatacct gaagccgctg gcagaacgct
    19081 ggttagtggt gggtgacaaa tttgttttag gttttgctca atccactgtc gagaaaagct
    19141 ttaaagatgc tggactggta gtagaaatta cgccgtttgg cggtgaatgt tcgcaaaatg
    19201 agatcgaccg tctgcgtggc atcgcggaga ctgcgcagtg tggcgcaatt ctcggtatcg
    19261 gtggcggaaa aaccctcgat actgccaaag cactggcaca tttcatgggt gttccggtag
    19321 cgatcgcacc gactatcgcc tcgaccgatg caccgtgcag cgcattgtct gttatctaca
    19381 ccgatgaggg tgagtttgac cgctatctgc tgttgccaaa taacccgaat atggtcattg
    19441 tcgacaccaa aatcgtcgcg ggcgcacctg cacgtctgtt agcagcgggt atcggcgatg
    19501 cgctggcaac ctggtttgaa gcgcgtgctt gctctcgtag cggcgcgacc accatggcgg
    19561 gcggcaagtg cacccaggcg gcgctggcac tggctgaact gtgctacaac accctgctgg
    19621 aagaaggcga aaaagcgatg cttgccgccg aacagcatgt agtaaccccg gcgctggagc
    19681 gcgtgattga agcgaacacc tatttgagcg gcgttggttt tgaaagcggc ggctggcggc
    19741 ggctcacgcg gtgcataacg gcctgaccgc tatcccggac gcgcatcact attatcacgg
    19801 tgaaaagtgg catttggtac gctgacgcag ctggtactgg aaaacgcgcc ggtggaggaa
    19861 atcgaaaccg tagctgcact tagccatgcg gtaggtttgc caataactct cgctcaactg
    19921 gatattaaag aagatgtccc ggcgaaaatg cgcattgttg caaaagcatc ttgtgcagaa
    19981 ggtgaaacca tccacaacat gcctggcggc gcgacgccag atcaggttta cgccgcactg
    20041 ctggtagctg accagtacgg tcagcgtttc ctgcaagagt gggaataacc tactccaaac
    20101 tcccggcttg tccgggagtt tgaacgcaaa attgcctgat gcgctacgct tatcaggcct
    20161 acgcaatctc tgcaatatat tgaatttgcg tgcttttgta ggccggataa ggcgttcact
    20221 ccgcatccgg cactctgtgc cagtaatctg aaactcccac gttgttcagg agttttttat
    20281 ccgcttctgg acattttctc cacagaacaa tcgttagccc ggaaggtcga atctgtgact
    20341 cttattttca cggacgaacc agatgaaagc ttcactggcg ctgctcagcc tgctaacggc
    20401 atttaccagc cactccttaa aatctcccgc cgttccacca accgtggtac aaatccaggc
    20461 caataccaac cttgccatcg cagacggtgc cagacagcaa attggcagca cgctatttta
    20521 cgatccggcg tatatgcagc ttacctatcc tggcggtgat gttccgcaag aacgcggtgt
    20581 atgttccgat gtggtgatcc gcgcattgcg cagccagaag gtcgatttgc agaaactggt
    20641 tcacgaagat atggcgaaga attttgctga gtacccgcaa aaatggaagt taaagcgccc
    20701 ggacagcaac atcgatcacc gtcgggtgcc taatctggaa acctggttta gccgccacaa
    20761 caaaacacgc cccatcagta aaaatgccgg tgactatcaa gcgggcgata ttgtctcctg
    20821 gcgattagat aacgggctgg cgcatattgg ggtggcgtca gatggcttcg cccgcgacgg
    20881 tacgccgctg gtgatacata acattggcgc aggcgcgcag gaagaagatg tgctgtttag
    20941 ctggcggatg gtggggcatt atcgttattt tgttaaataa gagatcgccc cacatgaaga
    21001 aaaaattcag atgcttttta cactgacagc ttcttttttc ttacggctca ccagcgccag
    21061 tgccagcaca atcagcacaa taccgctacc ttcaacggcc cccggattct caccgagcag
    21121 ccaccaggaa aagagtacgc cacaaacggg aacggccagt gtgcttaagc tggcaatact
    21181 ggcaggcaag tttttcagca caaacagcca taagctccac gctagcgccg tcgccagaat
    21241 cgcactgtag gccagcgccc agaacacggt gggctgccag tcaatttcac gttgcggcac
    21301 cagtaaagcg accacactca tcaccagcgc cgcatacagc atctgccagg atgttaacga
    21361 taataaatcc acgcgcggat gacgggcgta taggcgttta gcaacaatcg cgctcgcccc
    21421 ccaactaaca ccggagagga ttgccagcat ggcacttttc atcgaagaga aatcgagttg
    21481 ccacggctgc aacaccaaaa ataagccgaa agcggcaatc agaatcgcga aatattgccc
    21541 acgtcgcagg cgttcaccga gaaacaacgc ggcgaaaatc accacccaga acggcatggt
    21601 atagctcagg atcgccacct tccccgcacc tccgctgacc aacgcccact gcgccaaacc
    21661 aaccatcccg caggtttgta acagggcaat ggctaaggtg tatttaaacg gcgtcgggcg
    21721 cattccgcga ccacgtaata aaaggacgat gaataaaacg agagcgccga aaatgcagcg
    21781 taaggcggta aagtcgaagg caccgatgta actggtgact tgcttcatga aaatccagct
    21841 ataactccag ataagtgtga gcacaaccaa gccactgatt gccagtgggt tgctctttcc
    21901 tgcggcagac atgagagatc cagtgtgtag ttttcctgtg gacactatac gccggtaaag
    21961 aaacttcagc gactgcctga aagacagtcg ctgaagaagg tcagattaca gcaggtcgaa
    22021 acggtcgagg ttcatcactt tcacccatgc cgctacgaag tctttaacaa acttctcgtg
    22081 ggcatcgctg ctggcgtaaa cttccgctac tgcacgcagg acagagttag aaccaaatac
    22141 cagatccgca cggctggctg tgtatttcac ttcgccggtt tcgcggtcac gtccttcgaa
    22201 cagctctttc gattcgtcag tcgctttcca ctcgtaacgc atatccagca agttcacgaa
    22261 gaagtcattg ctcaatacgc caacgcggtc agtgaagacg ccgtttttgc tgccatcgaa
    22321 gttggcaccc agtacacgca tcccgcccac cagcgcagtc atttccggcg cggtcagcgt
    22381 cagttgctgt gctttgtcga tcagcagtga ctcggtggtg gaaacgtcca gacgagcgcg
    22441 atagttacgg aaaccgtcag caattggctc cagcagctca aacatctcaa tgtcagtctg
    22501 atcctgacgc gcatcaacgc gacccggcgc aaacggtaca tgaatgctca aacctgcggc
    22561 gcttgcggct ttctcaacac caaccacacc agccagcacg atgatatccg ccagcgaggc
    22621 tttaccagac tctttctgga ttttctccag aacaggcaga gcacgaacgg ctgcggcgtt
    22681 cacatcccag tcgcgctgcg gcattaatgc cagacgcgca ccgttggcac caccgcgttt
    22741 gtcgccacca cggaaggtag aagcagatgc ccaggccacc gataccagct cactaacaga
    22801 cagaccagaa tccgcaatcg cgaatttcag atcgataatg tcctgctcgg tcgggttgta
    22861 gatcggctgc ggcagcggat cttgccagat cagatcttct ttcggcactt ccggcccgat
    22921 gtagcgagat ttcggcccca tatccctgtg cgtcagtttg aaccaggcac gggcaaaggc
    22981 ttcgttgaac gcctgcggat cgttgaggaa acgacgagag atcttctcga actcaggatc
    23041 aaaacgcagc gtcaggtcgg tcaccagcat tgtcggttta cgtttcttcg acggatcgaa
    23101 cggatccggg ataatttccg gtgcgtctac cgcttcgaac tggattgctc cagccggact
    23161 gcgggtctgt acccactcat acttgaacag attctcgaag aaatagttgc tccactgggt
    23221 cggcgtctgg gtccagacta cttccagacc agaggtaatg gcatctgcgc caacgccgct
    23281 gccgtaagtg ctcgcccagc ctaaaccttg ttcttcaatc ggtgcagctt ctggatcagg
    23341 acctacgttt gatgtcggac cggcaccgtg ggttttaccc agcgtatgac cacccgcaat
    23401 cagcgccacg gtttcttcgt cgttcatgcc catgttgccg aaggtcgcgc ggatagctgc
    23461 tgccgcagaa agcggttcgc cgctgtgatc cgggccttcc gggttaacgt aaatcaggcc
    23521 catctcggtt gcacccagcg gtgctttcgc cagcgcttcc ggatgacggt gagtcagcca
    23581 ggctttttca tcaccccagt taacatccag atccggttcc cagacgtctt cacgaccggc
    23641 accaaaaccg aaggtacgga agccggagtt ttccagcgcc acgttacctg cgaggataaa
    23701 caggtcggcc caggagattt tctgaccata tttctgtttg attggccaca acaggcgacg
    23761 cgctttatcg aggcttacgt tatccggcca ggagttcagc ggtgcaaaac gttgctgacc
    23821 acgacccgcg ccgccgcgtc catcgattga acggtaagta cctgcgccgt gccaagccat
    23881 acgaataaac agaccggcgt agctgcccca gtcggctggc caccacggct gagattctgt
    23941 cagcagggct ttcagatctt ttttcaggcc gtagtaatct aatttgctga attctttgcg
    24001 gtagtcaaag tcctcaccca gcgggttaga acgattagaa tgttggttta acaggtcaac
    24061 acggagttga tttggccacc agtcgcgagt ggttgtgccc gcccccgcgc tctggtcgtg
    24121 accgccctga tggaacgggc atttgccagt ggctgtggtg ttatggatat cgtctgacgt
    24181 gctcatcaat gtgctcccct ctacagtgtt accgttacga tacacagcgt tagagagaag
    24241 ttataattga attaatccac ggatgcgata gttgagatct tacatattgt tggttaaaga
    24301 gatgtagatc aaattgatcg taattaagct tttttgatta tgtccgaatt cggacaaatg
    24361 ctttataaaa aagggtttta gcttcatatc cttcaagtaa tgagggagaa aacaaggctg
    24421 acaaccttat tttcccgccc tcatttcgag gcagcatttt gtgctctgtt taaaatttgt
    24481 gatcactgtg tgattttcac aaaagccaca ctatttataa accaggtcga acacccagcg
    24541 tatggcaaat cgcgtaactc atttcagcgc ggttaagcgt atagaagtgg aaatctttca
    24601 ctccttcacg gcttaaaatc ttcaccatat ccatggcaat attcgcgccc accagtttgc
    24661 gggtttcggc atcgtcatcc agcccgtcaa acatttgcgc catccacgcc ggaatacgca
    24721 cgttggtcat gtcggcaaat ttcttcgcct gtttaaagtt agataccggc aaaatgcccg
    24781 ggatgatttc cacatcaatg cccgccgata cacagcggtc acgaaaacgc aggtagcttt
    24841 cgacatcgaa gaagaactga gtaatcgcgc ggttggctcc ggcatccact ttgcgtttca
    24901 gattaagcaa atccgcctga gcgctttttg cttccgggtg aacttccgga tacgccgcca
    24961 cggatatatc gaaatctgcc acttctttta acagcgtcac caggtcagaa gcatacattt
    25021 ctggcttacc acttcccggc ggcagatcgc cacgcagtgc cacgatatga cgaataccgt
    25081 tattccagta gtcgcgtgca atggtgcgca gctcgtcggg cgtcgcatca atgcaagtaa
    25141 gatgcggtgc cgcttccaga ccagtgcgat ctttaatgcc tttaataatg ctgtgcgtac
    25201 ggtcgcgctc gccggagttc gcgccatagg tcaccgatac aaacttcggt ttcaggctgc
    25261 taaggcgatc gatggagttc cacagggtct gctccatttc actggtacgc ggcgggaaaa
    25321 actcgaacga aacattaatc tgcccctgga cttctgccag gctctgattc agggcatccc
    25381 gctggctggc gtgaaaaaag ctcatacctt acctcatcaa tcgcttgtag ttatatgttg
    25441 tgttgtgcac atctatccat ttagacgtcc agatgtaaag atgaaggaaa agccgaaggg
    25501 cgtcaaccga aaaatgacca atgatggtga ggaaaaatca gggaagatga aaaaacttca
    25561 ggatggtaag aaaaagaaaa tccgatatgc aaagcaaacc ggactgcaaa tataaactaa
    25621 ccatgtactt attgcttctt gccggattcg tcgtaaacgc ttcatccggc ctaattctat
    25681 gataattcaa tatatttcat tagaaaccat agacccgaaa acatgagtac cgggcattat
    25741 taaatttctg aaattacaac aactgtgcca gccggttgat atccgactga atcgccccgg
    25801 cggtgacgtc gcgcccagcg ccaggcccac ggatcaccag cggattatcg cggtaccagc
    25861 ggctttcgat ggcaaagacg ttatcgcatg gcagcagtga tgccagcgga tagtcttcac
    25921 gcaccgcctc cacgcccaca cgcgctttgc cgttggcatc gaaacgcgcc acgtagcgca
    25981 gcaccagccc catttcgcgg gccgcttcca gccgttgcac catctgctcg ttaagttcat
    26041 cgccattttc aaagaaatgg tcgatgctgc cgccttcgca atgagcaggc accagcgatt
    26101 ccacacgtac ctgatccggt tcaatgttgt aacccgcttc acgcgccaga atcaccagct
    26161 tgcgcattac gtctttgccg gagaggtcgt cacgcggatc aggctcggtt aagccctgct
    26221 gccacgcctg atccaccagc tcggtaaacg gcacgctacc gtcgaattgc aggaacagcc
    26281 aggagagcgt gccggagaag atcccactga tcgacaaaat ggtatcgccg ctgtcgatca
    26341 gatcgcgcac ggtatggttg atcggcaagc ccgcaccaac ggtggcgttg tatagccagt
    26401 gacgcccggt tttttcgaag gcgtcgtgga tctggcgata tttattgctg tcgcttgctc
    26461 ccgccagttt gttggcactg atgacgtgga aaccgtggct ggcgaaatcg agatactgat
    26521 cagcaagctg ctggctggcg gtaacgtcca acaccactaa atcatcatac ggatgggcgc
    26581 gcatccacag gaaaagcgac tcttcatcct gctcgaccgc ttcatcgttg aagaaggcta
    26641 acgcgcggct ggcgtccagc ccgtcataac tcaacagact gcggcggctg tccaccacgc
    26701 ctgccagcac aaattcaaag ccggtacgcg ccgaaagcgt gctctgctcg cgggcgaaca
    26761 gttccagcca gcgagagccg atattgcctt tgccgaacaa taccaggccg atgcgttttt
    26821 ctgcgcggaa gacggactga tgcagcccct gaatcaggct ttcggtcggg ccggtgcgca
    26881 gtactgccac cagactgatg ccgtcatcgg actgccacgt aaactcgacc ggctggcctt
    26941 tcagttgctg ccagaagcgg tggcaatgca gcgggttacg ggtgacaccc gccccgacca
    27001 tcgccaccag cgccagcccc tgacgcaggc gcagttctcc aggtaatccc gcttcgtcga
    27061 ggattttcag cgcactgtcg gccacttctg aggtgtagca aaattgcaac aactggcgat
    27121 cgttatgcac gccaaccgcc agtggacgca cctgggcacg tttcaggatc tggtcgatct
    27181 ctttatgcgc cagtttgaaa tcctgactgg cgggcacctg aaactcaatc aaacagacat
    27241 catcgtggct ggtgacaata cgcgcaccag taccggaggc cagcacgcgt tcaatgcgcg
    27301 tggaaccttg atccggcgtg tagctacagc gcagctgcag gtcgatttcg ctgccagaaa
    27361 ctggctgtaa agtacgggcg tgaagaacgg gagccgccag acgcgccagt tcgctggcct
    27421 catccagacg cagcaacggc agcaggcagg catctttcac tttacgcggg tcggcactgt
    27481 ataccccggc gacgtcgctc cagatggtta cacgagaaac acccgccagc gcaccgattt
    27541 gtgtcgcgga atagtcggaa ccgttacgcc ccagcaacac cgtttcaccg gcgttgttac
    27601 ggctgataaa cccggtcacc accagacgtt tgctcggatg ttgtaccagt agctgttgca
    27661 gcaacgggta agaaagccct tcatcaacct gcggttgcgc agcgcgttca gcgcgtaaaa
    27721 actcacgggc atcaagccag gccgctggca gcccttgttg attaagtacc gcagacatca
    27781 ggcgcgccga ccacacttca ccgtggccca ccacttccgc atacaccgca tcgttaatac
    27841 cactgtcgag caacgccgcc aggcgctcaa gatcgctgac aaaagcgcta atgaggctat
    27901 cggcttcttc agcgggtagc agaccgctaa tcagatcgca ctgatagcga cgcagcgttt
    27961 gttgaacctg atgcgcagag agacgatcgg tctggcttag tttcaaccag ttaatcaact
    28021 ggttagtggt gctaccggcg gcggaaacca ccatcatatc gtcaggctga gagaactccg
    28081 ccataatgcc cgcgacacgc aaataacact tcacatcagc cagactactg ccaccaaatt
    28141 tatgcagctg acgacctttc gcccctgcct gcgcaatcac actcattttt accccttgtt
    28201 tgcagcccgg aagccatttt ccaggtcggc aattaaatct tcgccatctt caataccggt
    28261 ggagatacgc agcagcgtct cggagatccc ggcggcagca cgcgcttctg gtgccatgcc
    28321 tgcatgtgtc atggttgcgg cgtgagagat taaactttcc actcccccta atgattccgc
    28381 cagcgtaaac aacgacagcc cgcccaggaa acgacacagc gtctgctcat cgccatccag
    28441 ttcaaaactc aacattgcgc caaagccttt ttgctgacgc gcggcaattt catgcccctg
    28501 attttccggc agcgacgggt gatacagttt tttcaccaac ggctgggttt gcagatattt
    28561 cacaatcgcc tgcgcgttgc gctgcgccag ctccatacgc ggcaccagcg ttcgcaaccc
    28621 gcgtagcagc agatagctgt caaacgcgcc gcccgtcacg ccaatattgt ttgcccacca
    28681 ggcaagttca gtgacaacgt ccgggtcttt agcaatcacc acgccagcca ccacgtcaga
    28741 gtgaccgttc agatatttcg tacatgaatg caacaccaga tcggcaccca atgccagcgg
    28801 attttgtaat gccgggctta agaaggtgtt atccaccacg ctcaccgccc cgacttccct
    28861 tgccagatgg cagattttcg caatatccac gacgcgtaac aatggattac ttgggctttc
    28921 taccagtacc agtttgggtt tttctgccag cgctgcccgt aatgcctgtt catcgccttg
    28981 atcaacaaac aacacgcgat agcaaccgcg tttcgccaga ctgtcgaaca ggcgatagct
    29041 accgccgtag cagtcgtgcg gcgcaaccag cagatcgcca ggtttcaaaa agacggtcgt
    29101 taccaggtga atcgcggaca tgccggtatt agtaagtact gcgccagcac cgccttccag
    29161 ttctgccagc gcacgctgaa ccacatcgcg cgttgggttg ccgcgacgcg agtaatcatg
    29221 cgcgcgcggt tcattaaatc cggtaaagtt ataggtgctg gaaagatgga tgggagggac
    29281 aacgcaacca tactgttcgt cgtcatttaa cccgctacgc actgcgatgg tggcctgttt
    29341 acgcgtcatg tgatgaagtt tcctgggctt tgtcggtgaa atgtcaggca ccagagtaaa
    29401 cattgtgtta atggacgtca atacatctgg acatctaaac ttctttgcgt atagattgag
    29461 caaatcccaa atagccgtta aaattatatg cattatcacg ccgacaggtg cattacacga
    29521 tgtcacggta acgcctgtac ggtaaactat gcgggtttac ggtcagtacc cacataaatt
    29581 gtggggtctg gtctcaattt attgacgaag aggattaagt atctcatggc tgaatggagc
    29641 ggcgaatata tcagcccata cgctgagcac ggcaagaaga gtgaacaagt caaaaagatt
    29701 acggtttcca ttcctcttaa ggtgttaaaa atcctcaccg atgaacgcac gcgtcgtcag
    29761 gtgaacaacc tgcgtcacgc taccaacagc gagctgctgt gcgaagcgtt tctgcatgcc
    29821 tttaccgggc aacctttgcc ggatgatgcc gatctgcgta aagagcgcag cgacgaaatc
    29881 ccggaagcgg caaaagagat catgcgtgag atggggatta acccggagac gtgggaatac
    29941 taaagcggaa aagagcggcg cggagtggaa tcgcctgatg cactacgctt atcaggccta
    30001 cgtcatattg caatttattg aatttgcacg aacttgtagg ccggataagg cgttcacgcc
    30061 gcatccggca taaacaacga gcacgttgtc tgcgacccac cgctttttat acatggacgt
    30121 ttaactatga aaaacaggct gctgatcctc agcctgctgg tttctgtacc tgcctttgcc
    30181 tggcagccgc aaactggcga catcatcttt caaatctctc gctcatcgca aagtaaagcg
    30241 atccaactgg caacccattc cgattacagc cacaccggta tgctggtgat gcgtaacaaa
    30301 aagccctacg tttttgaagc ggtcggcccg gtgaaataca ccccgctcaa gcagtggatt
    30361 gcccatggtg aaaagggcaa ctacgttgtt cgccgcgttg aaggcggact gagtgttgaa
    30421 caacagcaaa aactgacgca aacggcaaaa cgttatctcg gtaaaccgta tgatttcagc
    30481 ttctcatgga gcgacgatcg ccagtactgt tcggaagtgg tgtggaaggt ttaccagaac
    30541 gcgctgggaa tgcgcgtggg cgaacagcag aagttgaaag agtttgatct cagcaacccg
    30601 ctggtccagg cgaagctcaa agaacgctac ggtaaaaata ttccgcttga ggaaacggta
    30661 gtttcaccac aagccgtttt cgacgcgcca caactcacca cggttgccaa agagtggccg
    30721 ctgttttcgt ggtgatacga ccaaaattca ggcacaaaaa aagcgccgtg cggcgctttt
    30781 ttcggaaatc cggtcttatt tgctgcccgg gatgttgaaa cgcttgttga agcggtcaac
    30841 acggccaccg gtagcaacat cacgctgttt gccagtgaag aacgggtggc acttgctgca
    30901 cacgtcgagg ttcaggtcat gaccaacggt ggagcggatt tttattacgt taccgcaaga
    30961 gcagctagca gtaatttctt cgtatttcgg gtgaatatct tttttcatgg gaaaacctcg
    31021 gtttaaggcc gcgtcgctct tccagcccta acgccagaca ccacgcgatg ttaaaagtat
    31081 agcttcaata cgatcatttc gtacgaaggc gcgaaatcat acagaaatta accagcgtat
    31141 gcaaactgat ccgcactctt ctacggcaat gtgtatacta acccaccgaa tttcaagtca
    31201 ggatgatgct atgcccgttg cccacgttgc cttgcccgtt ccgcttcctc gtacctttga
    31261 ctatctgctg ccagaaggca tgacggttaa agctgggtgt cgcatgcgcg tgccgtttgg
    31321 caaacagcag gagcgcatcg ggattgtggt atcagttagc gatgccagcg aactgccgct
    31381 cactgagcta aaagcggtag tcgaagtgct ggatggtgag ccggtgttta ctcactccgt
    31441 ctggcgattg ctgctatggg cggcagatta ctatcatcat ccgattggcg atgtgctgtt
    31501 tcatgccttg ccgattttac tacgccaggg gcggcctgcg gcgaacgcgc cgatgtggta
    31561 ctggtttgcc actgaacaag gtcaggcggt ggatctgaac agcctgaaac gctcacccaa
    31621 gcaacaacag gcgctggcag cgttacggca aggcaaaatc tggcgcgacc aggttgccac
    31681 gctcgaattt aatgatgccg cgttgcaggc gctgcgcaaa aaaggtctgt gtgatttagc
    31741 aagtgaaaca ccagagttta gcgactggcg aacgaactat gccgtttctg gtgagcggtt
    31801 gcgattgaat accgaacagg ccaccgccgt tggcgcaatt catagcgcgg cagatacttt
    31861 ttctgcctgg ctgctggcgg gcgttaccgg ttccggtaaa acggaggttt atctcagtgt
    31921 actggaaaac gtgctcgctc agggcaaaca ggcgctggtg atggtgccgg aaatcggcct
    31981 gacaccgcaa actatcgccc gttttcgtga acggtttaat gcgcccgtgg aagttctgca
    32041 ttccggtctg aacgacagcg agcgtctttc ggcgtggctg aaagcgaaaa atggcgaggc
    32101 ggcgattgtg atcggcaccc gctccgcgct gtttacgccg tttaaaaatc tcggcgtgat
    32161 tgtcattgac gaagagcacg acagttccta caagcagcag gaaggctggc gctatcatgc
    32221 ccgcgacctg gcggtgtatc gtgcgcatag cgagcaaatc ccgattattc ttggctccgc
    32281 aacgcccgcg ctggaaacgt tgtgcaacgt ccagcagaaa aaataccgcc tgctgcgcct
    32341 gacgcgccgg gcggggaatg cgcgtccggc aattcaacac gtgctggatt taaaaggtca
    32401 gaaggtgcag gcaggtctgg ctccggcgtt aatcacccgt atgcgccagc atttacaggc
    32461 caacaatcag gttatcctct ttcttaaccg ccgtggcttt gcgcctgcgc tactgtgcca
    32521 cgactgcggc tggattgccg aatgcccacg ttgcgatcac tactacacgc tgcatcaggc
    32581 gcaacagcat ttgcgctgtc accactgtga cagtcagcgt ccagtgccgc gccagtgccc
    32641 ttcctgcggt tccacgcacc tggtacccgt ggggctgggc accgaacagc ttgaacagac
    32701 gcttgcgccg ctgttccctg atgtgcccat ttctcgtatc gaccgcgata ccaccagccg
    32761 caaaggggcg ctggaacagc aactggcgga agtacatcgc ggcggtgcga ggattttgat
    32821 cggcacacaa atgctggcga aaggtcacca ctttccggat gtgacgctgg tggcgttact
    32881 ggatgtggac ggcgcgctgt tttctgccga ttttcgctcg gcagagcgtt tcgctcagct
    32941 ttacacccag gtcgctggtc gtgccgggcg tgcgggtaaa caaggcgaag tggtgctgca
    33001 aacgcaccat ccggaacatc ctctgttgca aacgttgctc tataaaggct acgacgcctt
    33061 tgccgaacag gcgctggctg agcgacggat gatgcaactg ccgccatgga ccagccatgt
    33121 gattgtgcgt gcggaagatc ataacaatca gcatgcgccg ctgttcctgc aacaactgcg
    33181 taatctgatc ctctccagcc cgctggcaga cgacaaactg tgggttctcg gtccggttcc
    33241 ggcactggca cctaaacgtg gcggtcgctg gcgctggcag atattgttgc agcacccttc
    33301 ccgcgtgcgc ttgcaacaca tcattagtgg tacgctggcg ctcatcaata caataccgga
    33361 ttcccgcaag gtgaaatggg tactggatgt tgatccgatt gagggttaaa ccgcttacga
    33421 tgcgaggcgg atcgaaaaat tcaatattca tcacactttt catgaaaatt ctgtaaccgt
    33481 tttcacgcgc tatctgctaa aaatgttgcc gatgtgaagt aaacatggat gtagtacgcc
    33541 tgacgtgcca ggcgaggagt gagtgtgaaa gcgaagaagc aggaaactgc cgcgaccatg
    33601 aaagacgttg ccctcaaggc aaaagtctct acagcgactg tctcccgagc attaatgaat
    33661 cccgataaag tctcccaggc cacccgtaat cgggttgaaa aagcggcccg ggaagtgggt
    33721 tatttaccgc agcctatggg gcgcaacgtc aagcgtaatg aatcccgcac cattctggtg
    33781 attgtcccgg atatctgcga tcccttcttt agcgaaatta ttcgcggtat cgaagttacg
    33841 gcggcaaatc acggatatct ggtgctgatt ggcgactgtg cacatcaaaa tcagcaggaa
    33901 aaaaccttta tcgatttgat catcaccaag caaattgatg gcatgttgct gctgggttca
    33961 aggctgccgt ttgatgccag cattgaggaa cagcgtaatc tgccgccgat ggtgatggcg
    34021 aacgaatttg caccggaact ggagctgcct accgttcata tcgacaatct gaccgccgca
    34081 tttgatgcag taaattattt atatgagcaa gggcataaac ggattggctg tatagccggt
    34141 cccgaagaga tgccactgtg tcactaccgc ctgcaaggct atgttcaggc gctgcgtcgc
    34201 tgcggaatta tggttgatcc acaatacatc gcccgtggcg acttcacctt cgaagccgga
    34261 agcaaagcaa tgcaacagct gcttgatctt ccacaaccgc ctactgcagt cttctgccat
    34321 agcgatgtga tggcgctcgg cgcactttct caggcaaaac gccaggggct gaaagtcccg
    34381 gaagaccttt ccataatcgg ttttgataac atcgacctga cgcaattttg tgatccgccg
    34441 ctgacaacca tcgcgcagcc gcgttacgaa atcggtcggg aagctatgct gttattgctt
    34501 gatcaaatgc aggggcaaca cgttggcagt ggctctcgtt taatggactg cgaacttatc
    34561 atccggggat caacacgcgc gttaccttaa agtaaacctt taagactgcc gtgactggtc
    34621 aaaggcccgc cgcttaagta acatggcggg ctgacgaacg aataaataca gcgaaacgat
    34681 agtggcacaa cgagattatg tacgccgcag ccaaccggca ccttcgcggc gaaaaaagag
    34741 cacctcacgg aaaaagcaac gaaatctgcc tgcggtttct cccgctatgg tcgctattgc
    34801 tgccgccgtt cttgtgacct ttatcggtgg tctgtacttc attacgcatc acaagaaaga
    34861 agagtccgag acgctgcaaa gccagaaagt gaccggaaac ggactaccac caaaaccaga
    34921 agaacgctgg cgctacatta aagagctgga aagtcgccag ccgggtgtgc gtgcgcccac
    34981 agaaccttct gccggtggtg aagtgaaaac gccggagcaa ctgacaccag aacaacgtca
    35041 gcttcttgaa caaatgcagg ctgatatgcg ccagcagcca acgcagctgg ttgaggttcc
    35101 gtggaatgaa cagacgccgg aacagcgtca gcaaacgcta caacgccagc gtcaggcgca
    35161 gcaattagcg gagcagcaac gcctggctca gcagtcgcgt acgactgaac aaagctggca
    35221 gcagcagacg cgtacgtcgc aagccgcgcc ggtacaggcg caaccgcgcc agtctaaacc
    35281 agcttctact cagcagccgt accaggatct gctgcaaacc cctgcgcaca cgaccgcgca
    35341 gtcgaagccg cagcaagctg cgccggttgc tcgtgccgct gacgcgccaa aaccgacggc
    35401 ggagaaaaaa gacgaacgcc gctggatggt gcagtgcggt tcgttcagag gcgcggaaca
    35461 ggcagagacg gtacgtgctc agctggcgtt cgaaggattt gactcgaaaa tcaccaccaa
    35521 caatggctgg aatcgtgtgg tcattggccc ggtgaaaggc aaagagaacg cagacagcac
    35581 cctcaatcgg ttgaagatgg cgggtcatac aaactgcatt cggctcgccg ccgggggttg
    35641 aaaccctcaa aatccccccc atctataatt gcattatgcc ccgtactttt gtacggggtt
    35701 tgtactctgt attcgtaacc aaggggtcag ctcgtgacaa ctatagtaag cgtacgccgt
    35761 aacggccatg tggtcatcgc tggtgatggt caggccacgt tgggcaatac cgtaatgaaa
    35821 ggcaacgtga aaaaggtccg ccgtctgtac aacgacaaag tcatcgcggg ctttgcgggc
    35881 ggtactgcgg atgcttttac gctgttcgaa ctgtttgaac gtaaactgga aatgcatcag
    35941 ggccatctgg ttaaagccgc cgttgagctg gcaaaagact ggcgtaccga tcgcatgctg
    36001 cgcaaacttg aagcactgct ggcagtcgcg gatgaaactg catcgcttat catcaccggt
    36061 aacggtgacg tggtgcagcc agaaaacgat cttattgcta tcggctccgg cggcccttac
    36121 gcccaggctg cggcgcgcgc gctgttagaa aacactgaac ttagcgcccg tgaaattgct
    36181 gaaaaggcgt tggatattgc aggcgacatt tgcatctata ccaaccattt ccacaccatc
    36241 gaagaattaa gctacaaagc gtaaggatct cccatgtctg aaatgacccc acgcgaaatc
    36301 gtcagcgaac tggataagca catcatcggc caggacaacg ccaagcgttc tgtggcgatt
    36361 gctctgcgta accgctggcg tcgcatgcag ctcaacgaag agctgcgcca tgaagtgacc
    36421 ccgaaaaata tcctgatgat cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgt
    36481 ctggctaagc tggcgaatgc gccgttcatc aaagttgaag cgaccaaatt taccgaagtg
    36541 ggctacgtcg gtaaggaagt tgattctatt attcgcgatc tgaccgatgc cgccgtgaaa
    36601 atggtacgcg tccaggctat cgagaaaaac cgttatcgcg ctgaagaact ggcagaagaa
    36661 cgtattctcg acgtgctgat cccacctgct aaaaacaact ggggacagac tgaacagcag
    36721 caggaacctt ccgctgctcg tcaggcattc cgcaaaaaac tgcgtgaagg ccagcttgat
    36781 gacaaagaaa tcgagatcga tcttgccgca gcaccgatgg gcgttgaaat tatggctcct
    36841 ccgggcatgg aagagatgac cagccagctg cagtccatgt tccagaacct gggcggccag
    36901 aagcaaaaag cgcgtaagct gaaaatcaaa gacgccatga agctgctgat tgaagaagaa
    36961 gcggcgaaac tggtgaaccc ggaagagctg aagcaagacg ctatcgacgc tgttgagcag
    37021 cacgggatcg tgtttatcga cgaaatcgac aaaatctgta agcgcggcga gtcttccggt
    37081 ccggatgttt ctcgtgaagg cgttcagcgt gacctgctgc cgctggtaga aggttgcacc
    37141 gtttccacca aacacgggat ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg
    37201 ttccagattg cgaaaccgtc tgacctgatc ccggaactgc aaggtcggct gccaatccgc
    37261 gttgaactgc aggcactgac caccagcgac ttcgagcgta ttctgaccga gccgaatgcc
    37321 tctatcaccg tgcagtacaa agcactgatg gcgactgaag gcgtaaatat cgagtttacc
    37381 gactccggta ttaaacgcat cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac
    37441 atcggtgctc gtcgtttaca cactgttctg gagcgtttaa tggaagagat ttcctacgac
    37501 gccagcgatt taagcggtca aaatatcact attgacgcag attatgtgag caaacatctg
    37561 gatgcgttgg tggcagatga agatctgagc cgttttatcc tataatcgcg ttcaatcatt
    37621 ttcatcattg tttgatgggg ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa
    37681 caacaaatta gccgaactca ggcgtggctg gaaagtttac gacctaaaac cctccccctc
    37741 gcctttgctg caattatcgt cgggacggcg ctggcatggt ggcaaggtca cttcgatccg
    37801 ctggtcgccc tgctggcact aattaccgcc gggctattac agatcctttc taacctcgcc
    37861 aatgattacg gcgatgcggt aaaaggcagc gataaacctg accgcattgg gccgctacgc
    37921 ggcatgcaaa aaggggtcat tacccagcaa aagatgaaac gggcgctcat tattaccgtc
    37981 gtgctcatct gtctctccgg gctggcactg gttgcagtgg catgccatac gctggccgat
    38041 tttgtcggtt tcctgattct tggcgggttg tcgatcattg ccgctatcac ctacaccgtg
    38101 ggcaatcgtc cttatggtta tatcggtctg ggtgatattt ccgtactggt tttctttggc
    38161 tggttgagtg tcatggggag ctggtattta caggctcata cattgattcc ggcactgatc
    38221 cttccggcga ccgcatgcgg cctgctggca acggcagtac tgaatattaa taacctgcgt
    38281 gatatcaata gcgaccgcga aaatggcaaa aacacgctgg tggtgcgctt aggtgaagtg
    38341 aacgcgcgtc gttatcatgc ctgcctgctg atgggctcgc tggtgtgtct ggcgctgttt
    38401 aatctctttt cgctgcatag cctgtggggc tggctgttcc tgctggcggc accattactg
    38461 gtgaagcaag cccgttatgt gatgcgggaa atggacccgg tggcgatgcg accaatgctg
    38521 gaacgtactg tcaagggagc gttactgact aacctgctgt ttgttttagg gatattccta
    38581 agccagtggg cagcataact gacaaatatc aattaacaat tgatgatttt gccaacagcc
    38641 cacatagcgc gatatactga aaattctcgc agcaactgaa tgttaagcct atgaaatacg
    38701 atacttccga gctttgtgac atctatcaag aagatgttaa cgtcgtggaa ccgctgttct
    38761 ccaactttgg cggacgggcg tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg
    38821 acaacgggtt gctgtacgat ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg
    38881 atggcggtgg ttctgttcgt cgcgcactgg tcgatgctga actggcgcgt ctggcagtac
    38941 aaaatgaatg ggaaggtctg gtcatttacg gcgcggtgcg tcaggtagat gacctggaag
    39001 agttggatat cggcatccag gcgatggcgg caattccggt tggtgccgct ggcgaaggca
    39061 ttggcgaaag cgatgtccgc gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc
    39121 tttatgccga caataccggg attattcttt cagaagatcc gctggatatt gaatgataag
    39181 aaaggcaccg caaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt
    39241 acgcgaaaga tgaagcgtaa tcagacctct tccatgcgac ccagcagggc ctgcagacgt
    39301 tcctgccagc cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc
    39361 tgatgctggg cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg
    39421 atttccatct gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc
    39481 tcaaacactt ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa
    39541 aaatcctcgt cccgattacc ggtgacgcct taataaatac gagcgcactt tagttagctc
    39601 cgattgtatg aagccgcgcc atcgctgtcc agcggcacgc cttgcagatt acggtttgcc
    39661 atacttttca tccttctcct agtgacataa tccacatcaa tcgaaaatgt taataaattt
    39721 gttgcgcgaa tgatctaaca aacatgcatc atgtacaatc agatggaata aatggcgcga
    39781 taacgctcat tttatgacga agcacacaca ttttaagttc gatatttctc gtttttgctc
    39841 gttaacgata agttaacagc atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat
    39901 acaacaaaca ttaactcttc aggatccgat tatgagtcaa acatcaacct tgaaaggcca
    39961 gtgcattgct gaattcctcg gtaccgggtt gttgattttt ttcggtgtgg gttgcgttgc
    40021 agcactaaaa gtcgctggtg cgtcttttgg tcagtgggaa atcagtgtca tttggggact
    40081 gggggtggca atggccatct acctgaccgc aggggtttcc ggcgcgcatc ttaatcccgc
    40141 tgttaccatt gcattgtggc tgtttgcctg tttcgacaag cgcaaagtta ttccttttat
    40201 cgtttcacaa gttgccggcg ctttctgcgc tgcggcttta gtttacgggc tttactacaa
    40261 tttatttttc gacttcgagc agactcatca cattgttcgc ggcagcgttg aaagtgttga
    40321 tctggctggc actttctcta cttaccctaa tcctcatatc aattttgtgc aggctttcgc
    40381 agttgagatg gtgattaccg ctattctgat ggggctgatc ctggcgttaa cggacgatgg
    40441 caacggtgta ccacgcggcc ctttggctcc cttgctgatt ggtctactga ttgcggtcat
    40501 tggcgcatct atgggcccat tgacaggttt tgccatgaac ccagcgcgtg acttcggtcc
    40561 gaaagtcttt gcctagctgg cgggctgggg caatgtcgcc tttaccggcg gcagagacat
    40621 tccttacttc ctggtgccgc ttttcagccc tatcgttggc gcgattgtag gtgcatttgc
    40681 ctaccgcaaa ctgattggtc gccatttgcc ttgcgatatc tgtgttgtgg aagaaaagga
    40741 aaccacaact ccttcagaac aaaaagcttc gctgtaatat gactacggga caattaaaca
    40801 tgactgaaaa aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt
    40861 aatggatcac gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc
    40921 aaaaccaggt tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct
    40981 ggtagaagtg ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac
    41041 gaaccagcgt gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc
    41101 cattgtctgg cagtgccgtc gtaccgcaga aatctgcgag catttaaaac gtgacggttt
    41161 agaagattat atccgcagca ataccggtct ggtgattgac ccgtactttt ctggcaccaa
    41221 agtgaagtgg atcctcgacc atgtggaagg ctctcgcgag cgtgcacgtc gtggtgaatt
    41281 gctgtttggt acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt
    41341 gaccgattac accaacgcct ctcgtaccat gttgttcaac atccataccc tggactggga
    41401 cgacaaaatg ctggaagtgc tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc
    41461 ttccgaagta tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc
    41521 cgggatcgcc ggtgaccagc aggccgcgct gtttggtcag ttgtgcgtga aagaagggat
    41581 ggcgaagaac acctatggca ctggctgctt tatgctgatg aacactggcg agaaagcggt
    41641 gaaatcagaa aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtgaacta
    41701 tgcgttggaa ggtgcggtgt ttatggcagg cgcatccatt cagtggctgc gcgatgagat
    41761 gaagttgatt aacgacgcct acgattccga atatttcgcc accaaagtgc aaaacaccaa
    41821 tggtgtgtat gtggttccgg catttaccgg gctgggtgcg ccgtactggg acccgtatgc
    41881 gcgcggggcg attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac
    41941 gctggagtct attgcttatc agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg
    42001 tatccgtctg cacgccctgc gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca
    42061 gttccagtcc gatgttctcg gcacccgcgt tgagcgcccg gaagtgcgcg aagtcaccgc
    42121 attgggtgcg gcctatctcg caggcctggc ggttggcttc tggcagaacc tcgacgagct
    42181 gcaagagaaa gcggtgattg agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa
    42241 ttaccgttac gcaggctgga aaaaagcggt caaacgcgcg atggcgtggg aagaacacga
    42301 cgagtaatgt caggcacctg aatcgattgc ctgatgctga tcgcctgatg cgacgcttac
    42361 gcgtcttatc aggcctacga gccaatgcca ttttgtgtag gcgggataag gcgttcacgc
    42421 cgcatccggc atcacgcaga ctctgaaacc gtgcgggggc aaccccgcac acatcaataa
    42481 tcccaccctt cccctgtgct acacttcgcg ccattcctta ctgcttagag tttgctatga
    42541 gacgagaact tgccatcgaa ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca
    42601 aatggttagg acgcggcgat aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta
    42661 ttatgctcaa ccaggtcaac attgacggca ccatcgtcat tggtgaaggt gaaatcgacg
    42721 aagcaccgat gctctacatt ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata
    42781 ttgctgttga tccgattgaa ggcacgcgca tgacggcgat gggccaggct aacgcgctgg
    42841 cggtgctggc agtaggcgat aaaggctgct tcctcaatgc gccggatatg tatatggaga
    42901 agctgattgt cgggccggga gccaaaggca ccattgatct gaacctgccg ctggcggata
    42961 acctgcgcaa tgtagcagcg gcgctcggca aaccgttgag cgaactgacg gtaacgattc
    43021 tggctaaacc acgccacgat gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat
    43081 ttgctattcc ggacggcgac gttgcggcct caattctcac ctgtatgcca gacagcgaag
    43141 ttgacgtgct gtacggtatt ggtggcgcgc cggaaggcgt ggtttctgcg gcggtgatcc
    43201 gcgcattaga tggcgacatg aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca
    43261 acgaagagaa tcgtcgcatt ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg
    43321 aagccggtaa agtattgcgt ctgggcgata tggcgcgcag cgataacgtc atcttctctg
    43381 ccaccggtat taccaaaggc gatctgctgg aaggtattag ccgcaaaggc aatatcgcga
    43441 ctaccgaaac gctgctgatc cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc
    43501 actatctgga tcgcaaagac ccggaaatgc aggtgcacat tctctgagtg atttgatcga
    43561 ttgagccttc cagtccttcg ggactggaat ttttttgttc ggagaacgaa gataaggcaa
    43621 gtcaatcaaa acaggagaaa aacatggctg attgggtaac aggcaaagtc actaaagtgc
    43681 agaactggac cgacgccctg tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg
    43741 ccgggcaatt taccaagctt ggccttgaaa tcgacggcga acgcgtccag cgcgcctact
    43801 cctatgttaa ctcgcccgat aatcccgatc tggagtttta cctggtcacc gtccccgatg
    43861 gcaaattaag cccacgattg gcggcactga aaccgggcga tgaagtgcag gtggttagcg
    43921 aagcagctgg cttctttgtt ctggatgaag taccggactg cgaaacgcta tggatgctgg
    43981 caaccggtac agcgattggc ccttatttat cgattctgca actaggcaaa gatttagatc
    44041 gcttcaaaaa tctggtcctg gttcacgccg cacgttatgc cgccgactta agctatttgc
    44101 cactgatgca agaactggaa aaacgctacg aaggcaaact gcgcattcag acggtggtca
    44161 gtcgggaaac ggcagcgggg tcgctcaccg gacggatacc ggcattaatt gaaagtgggg
    44221 aactggaaag cgcgattggc ctgccgatga ataaagaaac cagccatgtg atgctgtgcg
    44281 gcaatccaca gatggtgcgc gatacacaac agttgctgaa agagacccgg cagatgacga
    44341 aacatttacg tcgccgaccg ggccatatga cagcggagca ttactggtaa gcggttactt
    44401 atcgataaac ggcacgatga gcaaatccgc actcatctta ttgatcatcc cgcgatatgc
    44461 cggcatcaaa cggttgataa atgagtgatg atgaccacag acaaggaggt cgcactgctc
    44521 tttttgcata atttccagca gtgtttccgg catttctccg cgttcaatac gcagttttgt
    44581 cttcggccat tgaatatttt tcgtcagttt atacagcttg ttatccgact tattcttcaa
    44641 caattgaaga atatcttctg ttgcagggaa gtagataccc ggatacaact cgcttaagcc
    44701 atcatcaata tgaattaacg tcaggtgagc gtcattatgt ctggcgagct ccagggcttt
    44761 attcaccagt aaggcatctt cttcattccc ggaaattgcc acgccaatgt gtttataagc
    44821 catgtttaac tccttctaaa gcaactccat caagctaacg aacgcagtga taactcaaaa
    44881 ataatcatct cggcctggca tgagaaagtg aaggccgcat caagctcaac ttgcccctcc
    44941 agatccttaa tcttataggc cacgctggcc ccttcatctg cagaggatgc agcggcggca
    45001 agctctttca gtttggtcat aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa
    45061 aacttactgg tacagttgtc gttatcaatc accgtaccga tatcaatcgc acatccttta
    45121 gtactgcatt tatctacgac atctttcata gggcacctct gtatattcgc ctctctgtcg
    45181 attacgactt aagaataatg cgccgttaag ggaagaacaa acaagtaatg atcttatgga
    45241 ttaccatttt ttttgatatt gcttaaggtg aagagttcag aaggattttc cgggtattgt
    45301 catttgcatc gtggaaataa caatcccgga aaaaaatagc agtaagagat atattaaagg
    45361 ttaattactt gatttattgt cggctttata cttcacatcc tgagtatctt taccatattt
    45421 attttcgcct tgtgtgccaa caaacgcacc aagatcgata agcatcatca ccagaatcaa
    45481 cgtcgggaca aaacgcccca ccgcccattg ccagacaccc ggtaaaatcg ctcagttacc
    45541 cgccagcagc atccacgcca caatcatcag aaatgcccat gcgccggaac gcccgcgatc
    45601 atgcaagcgc ttaacagtta ctgccgctgt tggccagagc aagcacacaa ggcaaaacgc
    45661 cgcggtctga atatcgagta gattcttacc cgccagtgag aaaagcacca gcatgcctgc
    45721 gaaccacagg cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa
    45781 taaccattgc tgtatggtca tgtaagttcc ttgatggttg tcttttccag gattctaccc
    45841 ttttgacaag ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc
    45901 aatgaagcca gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac
    45961 ggcgcatgca caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc
    46021 tactttcgat ctctccatca gccagtttcg agaagacttt aacagccaga atcccagcct
    46081 gccactgaac gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc
    46141 tgcaagtaaa attaacgaga acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa
    46201 aatcaaaagc attcaaatga cctggctacc catccagggg ccagagcaaa aagcagcgaa
    46261 agcgaaagct caggaataca tggcagcagt gatccgcaca ctcaccccat taatgaccaa
    46321 aacacaaagc cagaaaaaac tgcagtcgct actaacggcg gggaaaaaca aacgttatta
    46381 caccgagaca gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac
    46441 cttcgctgtt gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg
    46501 acaaaaagca aagcctttgt gccgatgaat ctctatactg tttcacagac ctgctgccct
    46561 gcggggcggc catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct
    46621 ttagtgatgg gtaactggaa actgaacggc agccgccaca tggttcacga gctggtttct
    46681 aacctgcgta aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa
    46741 atgtacatcg atatggcgaa gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa
    46801 aacgtggacc tgaacctgtc cggcgcattc accggtgaaa cttctgccgc tatgctgaaa
    46861 gacatcggcg ctcagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag
    46921 tctgacgaac tgattgcgaa aaaattcgcg gtgctgaaag agcagggtct gactccggtt
    46981 ctgtgcatcg gtgaaaccga agctgaaaac gaagcgggaa aaactgaaga agtttgcgca
    47041 cgtcagatcg acgcggtact gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc
    47101 gcttacgaac ctgtatgggc aatcggtact ggcaaatctg caactccggc tcaggcacag
    47161 gctgttcaca aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa
    47221 gtgatcattc agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag
    47281 ccagacatcg acggcgcgct ggttggcggt gcttctctga aagctgacgc tttcgcagtg
    47341 atcgttaaag ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata
    47401 tccggcactt actttcctta actcctcgcc ttaacgcaaa atctcacact catgatcctg
    47461 gatttcctcg gctgaagcac ggttcagcgt cagtagattt cgttgtgtcg ccagcaacac
    47521 aaacgaatta tcactctgcc gtaccatcgc cagcccgtag cttcccatat gttcccgcgc
    47581 ctcaggcact tcttctgcaa gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt
    47641 tacccgacgc gccaggtatt catgcccgcg caaaccacct ggcagtggca accagcggct
    47701 gctgatgttc gccagatttt tatccagttg ttcgcgcaca tcaggacgaa tacaagagat
    47761 atgaatatga aaatggtttt gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc
    47821 gcgatcggga accggctggc cgtatttttt gctcataaaa tcacgcgcct gccaggccaa
    47881 ccaaaagaag ttcggcgttg aaggatcggt caacaaagga ctttcagtac cgttaatacg
    47941 atacgttggc atcaacagat attgcagtgg accattaaga tcttttaaaa ccacgtatcc
    48001 ggcattgggt ttgacttccg cacatggcga aggattttga ttttgctgct gattgggcaa
    48061 acattcctca aggacaatct tacgtaatgt atccgactct tcaccggtta atttccagta
    48121 accaataccg gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt
    48181 tttcatcttt ttttccctgt acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa
    48241 taaaaaaccc ggtcgcgaga aaccaccggg tttgataatt atcctaggga aatcagcgtt
    48301 tgctgatctg atcgaacgta ccgccgttag caaaatgctc tttttgcgct ttcgtccagc
    48361 cgccgaactc ttcatcaatg gtgaataact tcagctttgg aaacgcattt tcgtactttt
    48421 tcgctacttc agcgtcgcgc ggacggtagt agtttttcgc ggcaatttcc tgaccttctg
    48481 gcgagtagag atatttcagg taggcttccg ccacctcttt ggtgcctttt ttctcgacca
    48541 ctttatcgac caccgacacc gttggctctg cgaggataga ctcactcggc gtgacgattt
    48601 cgaatttatc tttccccagt tcattcgctg ccagcagagc ttcgttttcc caggcgatca
    48661 gtacatcgcc aatcccgcgc tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca
    48721 gaacttcgac gtttttatac agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt
    48781 tgttgtgatg cagcgcgtag ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc
    48841 ttttcggatt aggcgtgatc accgaaacac ccggtttaat cagatcgttc cagtcatgga
    48901 tctgcttcgg attgccctta cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt
    48961 tatccggcag acgtttgatc cactctttat caatccgccc gcgttccgca atcgcgtcca
    49021 cgtcataggc cagagccagc gtgacaacat cagcttcaat accgttgatt accgacgtcg
    49081 cttgtttacc tgaaccaccg tgtgactgac gaatcaccac gttatcacca gtttgctgtt
    49141 tccagtggga gctgaatgcc ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg
    49201 aaacgttaag aagctgaata tcctttgcca taacgctggt tgccgccagc aaaaatgtta
    49261 accctacgcc ccacttgttc atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc
    49321 gtgccagaag gttaaccaaa cattaaagaa taaaaaaaga ttggctataa cttgcgggta
    49381 tatgttgagg gattaaaaag gcggaagaaa tccgcctcat attgctgaca aagtgcgctt
    49441 tgtccatgcc ggatgcggcg cgaacgcctt atccggccta caaaagtttg caaattcaat
    49501 aaattgcaga attcatgtag gcctgataag cgaagcgcat caggcatttt tgcttctgtc
    49561 atcggtttca ggctaaagga atctgccttt ttccgaaatc attaatacag ttttttcgcg
    49621 cagtccagcc agtcaccttt gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg
    49681 tggtgaacca gctgttcgtt ctggatacct acacaacgac cgccgtaacc tgccagcagc
    49741 agatcgatag cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg caccggagaa
    49801 ccaccgcgct ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt ttctttctcg
    49861 atgaaatgcg ccagttcgtc aacatcacac atatgttcgg taatcgccac gatcgcgtgt
    49921 tttttacctt tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg gctgaattca
    49981 acttccggaa ctacaacgaa ttcacagcca ccggcaatgg ccgcagccag ggtcagatcg
    50041 ccacaataac ggcccatcac ttccaccacg gaaatacgct ggtgagaaga agaggtgtca
    50101 cgcagacggt cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag
    50161 tcagtgcctt tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg gaagcccatt
    50221 tcggtcagac gcattgcacc catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg
    50281 ataccacgtt ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc
    50341 gggaaacgcg cagaaccgag gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa
    50401 cggtctagct gtaccatacg gtcttcatac agacccagat agccgtcata aatacccatt
    50461 acttccagac cttctgtcag cgcagaacga acaaccccgc gaattgcggc gttcatgcct
    50521 ggcgcatcac cgccgcttgt caacacacct attttcttaa tcatgactac ctctgaactt
    50581 tggaatgcaa aatgaaatct gttgccggaa gtcttcttgc acatcgaagt gatccaacga
    50641 atgtgcaaat agtataacaa tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg
    50701 gtattttata cacaaaatgc gggtctggct ctcttttata ctgattatga aagcatagac
    50761 cgtttaccct ccctgggtac gacggaacag gggtcctgat ggataatcac atccgatccc
    50821 ggaaaacgcc gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa
    50881 ggcagagagt cttccatttc caaatgaatc tgaataaagc gggtcggccc tgactgccgc
    50941 gtgcgaagat cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc aataatttct
    51001 tgccgttctt catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc atatcccatg
    51061 cgtaacgcgc tatataaaat atagatgccg attcccaatg caaacagagc atcggcgcga
    51121 tgccagccgt accaagacaa ccccagcgcc agcagaattg cgccgttcat cataacatca
    51181 gactggtaat gtagcatatc agcccgcacc gcctggcttt gcgtccggcg caccacccac
    51241 cgctgaaacg agacaaggat aatcgtacaa attagcgcca caattgtcac gataaccccg
    51301 acgcctggat ctgtcatcgg tgttggagat accagatgtt gaatacccgt caaaaacagg
    51361 aatagtgccg aaccggagat aaacatactt tgcgccagcg ccgcgaggga ctcagcttta
    51421 ccgtgaccaa acgagtgatt atcgtcggca ggttgcaggg aatatcgcac caccagtaaa
    51481 ttcgtcaacg acgcgccgat atccaccagc gaatccacca gcgcggcgag aatactcacc
    51541 gacccggtat accaccatgc aaaaatttta atcagcaata gcaacgaagc catcgccgtc
    51601 gcagcaatcg ccgcccgact gaccagccgt ccataagatt gattcataaa tactcccgct
    51661 atcaactgac gctagtataa cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa
    51721 ttgaggataa aaaaaacccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag
    51781 gaaaacaggg tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa
    51841 cttttttgcc attgcgtcac gtcacgcaac tgctccattc gttgttgatg tttctcgttt
    51901 aaaaccgctt gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc
    51961 tcaacctgac gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt
    52021 tcatcaaaat tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca
    52081 ggaggctgtt cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg
    52141 gttaaactta tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc
    52201 ggatgccagt tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc
    52261 gttgaggcca tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc
    52321 gcgattcaac gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa
    52381 caacgtaaag tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta
    52441 aacaatgaat aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga
    52501 gctgctcgag atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga
    52561 tcttctggac gacagcattg atttactttt gcttgacgta atgatgccga agaaaaatgg
    52621 tatcgacaca ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc
    52681 gcgcggcagt gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc
    52741 gaaaccgttt aatgatcgtg agctggtggc acgtattcgc gcaatcctgc gccgttcgca
    52801 ctggagcgag caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt
    52861 agtgctgaat ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg
    52921 tactgagttt accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga
    52981 acatttaagc caggaagtgt tgggcaaacg cctgacgcct ttcgaccgcg ctattgatat
    53041 gcacatttcc aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa
    53101 aaccttgcgt ggtcgcggct atctgatggt ttctgcttca tgataggcag cttaaccgcg
    53161 cgcatcttcg ccatcttctg gctgacgctg gcgctggtgt tgatgttggt tttgatgtta
    53221 cccaagctcg attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg
    53281 atgattgagc agcatgtcga agcggagctg gcgaacgatc cgcccaacga tttaatgtgg
    53341 tggcggcgtc tgttccgggc gattgataag tgggcaccgc caggacagcg tttgttattg
    53401 gtgaccaccg aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac
    53461 tttattggtc aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa
    53521 ctggtcggtc cgttctccgt gcgtgatggc gaagataatt accaacttta tctgattcgt
    53581 ccggccagta gttctcaatc cgatttcatt aacttactgt ttgaccgccc gctattactg
    53641 ctgattgtca ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca
    53701 aaaccggcgc gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag
    53761 cacccggaac tggaagcggg gccacaggaa ttccttgccg caggtgccag ttttaaccag
    53821 atggtcaccg cgctggagcg catgatgacc tctcagcagc gtctgctttc tgatatctct
    53881 cacgagctgc gcaccccgct gacgcgtctg caactgggta cggcgttact gcgccgtcgt
    53941 agcggtgaaa gcaaggaact cgagcgtatt gaaaccgaag cgcaacgtct ggacagcatg
    54001 atcaacgatc tgttggtgat gtcacgtaat cagcaaaaaa acgcgctggt tagcgaaacc
    54061 atcaaagcca accagttgtg gagtgaagtg ctggataacg cggcgttcga agccgagcaa
    54121 atgggcaagt cgttgacagt taacttcccg cctgggccgt ggccgctgta cggcaatccg
    54181 aacgccctgg aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg
    54241 aagattgaag tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat
    54301 ggtcctggcg ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat
    54361 gaagcacgcg atcgtgaatc tggcggtaca ggattggggc tggcgattgt tgaaaccgcc
    54421 attcagcagc atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg
    54481 gtgatttggt tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc
    54541 gtttacgccg catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc
    54601 aggcctacac tccgggggtt attttcccca caaccggcgg gaaaaatctt cgatcttgcc
    54661 gctcagtcgg cgcttttgca tcgtccgcgt ccagcttttc gataacccgg cggcggagag
    54721 taaacggtga tactgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg
    54781 cacggtcaca tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc
    54841 tggcgctatc acgctataca accaccccac cttacctgtg ttttgcatca actgcgctat
    54901 atcgctgata tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg
    54961 aattaatgcc tctccccagc ggaaaatatc gcccatatga acattacttt ccgtgagacc
    55021 gtcggttgag agattttcgc caaacgcagg cgcaacaaac aactccgcct gttccggaaa
    55081 ttcccgcacc cagtagagat agtgctcacg aggataatga cacagcgctc tgtctggccc
    55141 gccgtgaacc tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa
    55201 ctcaccatca acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat
    55261 cttgcctgtg tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc
    55321 cattagacca tatttatttt atgtcagccg tgctgttttc gcggtaagta tccaggcagg
    55381 aactcaatag caaaccggac gaacgttagt tcatccggct gtgggga
//
LOCUS       DAJVRL010000014        53312 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVRL010000014 DAJVRL010000000
VERSION     DAJVRL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.014"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(11..322)
                     /locus_tag="OQN02_000832"
     CDS             complement(11..322)
                     /locus_tag="OQN02_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1641737.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OQN02_000833"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQN02_000833"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OQN02_000834"
     CDS             701..1054
                     /locus_tag="OQN02_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1641738.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OQN02_000835"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OQN02_000835"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1641739.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OQN02_000836"
     CDS             complement(2810..3280)
                     /locus_tag="OQN02_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS1641740.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OQN02_000837"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OQN02_000837"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS1641741.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OQN02_000838"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OQN02_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1641742.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OQN02_000839"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OQN02_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1641743.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OQN02_000840"
     CDS             complement(5283..8432)
                     /locus_tag="OQN02_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1641744.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OQN02_000841"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OQN02_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1641745.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OQN02_000842"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OQN02_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1641746.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OQN02_000843"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OQN02_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1641747.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OQN02_000844"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OQN02_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1641748.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OQN02_000845"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OQN02_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1641749.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OQN02_000846"
     CDS             14911..15288
                     /locus_tag="OQN02_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1641750.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OQN02_000847"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OQN02_000847"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1641751.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OQN02_000848"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OQN02_000848"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1641752.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OQN02_000848"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OQN02_000849"
     CDS             18099..18428
                     /locus_tag="OQN02_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1641753.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OQN02_000850"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OQN02_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1641754.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OQN02_000851"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OQN02_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1641755.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OQN02_000852"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OQN02_000852"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1641756.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OQN02_000853"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OQN02_000853"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1641757.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OQN02_000854"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OQN02_000854"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS1641758.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OQN02_000855"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OQN02_000855"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1641759.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OQN02_000856"
     CDS             complement(25481..27157)
                     /locus_tag="OQN02_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1641760.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OQN02_000857"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OQN02_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1641761.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OQN02_000858"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OQN02_000858"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1641762.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OQN02_000859"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OQN02_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1641763.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OQN02_000860"
     CDS             complement(31205..31999)
                     /locus_tag="OQN02_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1641764.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OQN02_000861"
     CDS             32137..32478
                     /locus_tag="OQN02_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1641765.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OQN02_000862"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OQN02_000862"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1641766.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OQN02_000863"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OQN02_000863"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1641767.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OQN02_000864"
     CDS             36394..37686
                     /locus_tag="OQN02_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1641768.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38245
                     /gene="cueR"
                     /locus_tag="OQN02_000865"
     CDS             37811..38245
                     /gene="cueR"
                     /locus_tag="OQN02_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1641769.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38219..38677)
                     /locus_tag="OQN02_000866"
     CDS             complement(38219..38677)
                     /locus_tag="OQN02_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1641770.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OQN02_000867"
     CDS             complement(38674..39591)
                     /locus_tag="OQN02_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1641771.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OQN02_000868"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OQN02_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1641772.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OQN02_000869"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OQN02_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1641773.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OQN02_000870"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OQN02_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1641774.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OQN02_000871"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OQN02_000871"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1641775.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OQN02_000872"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OQN02_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OQN02_000873"
     CDS             43550..44236
                     /locus_tag="OQN02_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1641776.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="OQN02_000874"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="OQN02_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OQN02_000875"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OQN02_000875"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1641777.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OQN02_000876"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OQN02_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCS1641778.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="OQN02_000877"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="OQN02_000877"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCS1641779.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="OQN02_000878"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="OQN02_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCS1641780.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="OQN02_000879"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="OQN02_000879"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCS1641781.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..53216
                     /locus_tag="OQN02_000880"
                     /pseudo
     CDS             52938..53216
                     /locus_tag="OQN02_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
     gene            complement(53230..>53312)
                     /locus_tag="OQN02_000881"
     CDS             complement(53230..>53312)
                     /locus_tag="OQN02_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1641782.1"
                     /translation="KSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT        12854 a        13714 c        14041 g        12703 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cttggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tagaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 tgcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggggatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatttgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtcttgcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggagttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatctcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt cgttggtaat
    53221 gactccaact tactgatagt gttttatgtt cagataatgc ccgatgacct tgtcatgcag
    53281 ctccaccgat tttgagaacg acagtgactt cc
//
LOCUS       DAJVRL010000015        52075 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVRL010000015 DAJVRL010000000
VERSION     DAJVRL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.015"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="OQN02_000882"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="OQN02_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1641783.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="OQN02_000883"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="OQN02_000883"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1641784.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="OQN02_000884"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="OQN02_000884"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1641785.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="OQN02_000885"
     CDS             complement(5906..6949)
                     /locus_tag="OQN02_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1641786.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="OQN02_000886"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="OQN02_000886"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1641787.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="OQN02_000887"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="OQN02_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1641788.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="OQN02_000888"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="OQN02_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="OQN02_000889"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="OQN02_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS1641789.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="OQN02_000890"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="OQN02_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1641790.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="OQN02_000891"
     CDS             15474..15830
                     /locus_tag="OQN02_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1641791.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="OQN02_000892"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="OQN02_000892"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1641792.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="OQN02_000893"
     CDS             16989..17189
                     /locus_tag="OQN02_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1641793.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="OQN02_000894"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="OQN02_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1641794.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="OQN02_000895"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="OQN02_000895"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1641795.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="OQN02_000896"
     CDS             complement(20101..20964)
                     /locus_tag="OQN02_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1641796.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="OQN02_000897"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="OQN02_000897"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1641797.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="OQN02_000898"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="OQN02_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1641798.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="OQN02_000899"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="OQN02_000899"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1641799.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="OQN02_000900"
     CDS             24133..24705
                     /locus_tag="OQN02_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1641800.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     TFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="OQN02_000901"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="OQN02_000901"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1641801.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="OQN02_000902"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="OQN02_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1641802.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="OQN02_000903"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="OQN02_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="OQN02_000904"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="OQN02_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1641803.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="OQN02_000905"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="OQN02_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCS1641804.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="OQN02_000906"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="OQN02_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1641805.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="OQN02_000907"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="OQN02_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1641806.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="OQN02_000908"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="OQN02_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="OQN02_000909"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="OQN02_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1641807.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="OQN02_000910"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="OQN02_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1641808.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="OQN02_000911"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="OQN02_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1641809.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="OQN02_000912"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="OQN02_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCS1641810.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="OQN02_000913"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="OQN02_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="OQN02_000914"
     CDS             complement(39099..40160)
                     /locus_tag="OQN02_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1641811.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="OQN02_000915"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="OQN02_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1641812.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="OQN02_000916"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="OQN02_000916"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1641813.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="OQN02_000917"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="OQN02_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1641814.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="OQN02_000918"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="OQN02_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1641815.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="OQN02_000919"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="OQN02_000919"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1641816.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="OQN02_000920"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="OQN02_000920"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1641817.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="OQN02_000921"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="OQN02_000921"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1641818.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="OQN02_000922"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="OQN02_000922"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1641819.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="OQN02_000923"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="OQN02_000923"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1641820.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="OQN02_000924"
     CDS             complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="OQN02_000924"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1641821.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12158 a        13738 c        13519 g        12660 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aaacgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacagt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgctt
//
LOCUS       DAJVRL010000016        49536 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVRL010000016 DAJVRL010000000
VERSION     DAJVRL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.016"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..1156)
                     /gene="setB"
                     /locus_tag="OQN02_000925"
     CDS             complement(<1..1156)
                     /gene="setB"
                     /locus_tag="OQN02_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1641822.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLF"
     gene            1524..2654
                     /gene="fruB"
                     /locus_tag="OQN02_000926"
     CDS             1524..2654
                     /gene="fruB"
                     /locus_tag="OQN02_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1641823.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2654..3592
                     /gene="fruK"
                     /locus_tag="OQN02_000927"
     CDS             2654..3592
                     /gene="fruK"
                     /locus_tag="OQN02_000927"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1641824.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3609..5300
                     /gene="fruA"
                     /locus_tag="OQN02_000928"
     CDS             3609..5300
                     /gene="fruA"
                     /locus_tag="OQN02_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1641825.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5724..6665
                     /locus_tag="OQN02_000929"
     CDS             5724..6665
                     /locus_tag="OQN02_000929"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1641826.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6653..7591
                     /gene="psuG"
                     /locus_tag="OQN02_000930"
     CDS             6653..7591
                     /gene="psuG"
                     /locus_tag="OQN02_000930"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1641827.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7685..8935
                     /locus_tag="OQN02_000931"
     CDS             7685..8935
                     /locus_tag="OQN02_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1641828.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="OQN02_000932"
                     /pseudo
     CDS             complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="OQN02_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="OQN02_000933"
                     /pseudo
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="OQN02_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10872..12122
                     /locus_tag="OQN02_000934"
     CDS             10872..12122
                     /locus_tag="OQN02_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1641829.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13266)
                     /locus_tag="OQN02_000935"
     CDS             complement(12178..13266)
                     /locus_tag="OQN02_000935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1641830.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="OQN02_000936"
     CDS             complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="OQN02_000936"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1641831.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14200..15249)
                     /locus_tag="OQN02_000937"
     CDS             complement(14200..15249)
                     /locus_tag="OQN02_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1641832.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15348..16229
                     /locus_tag="OQN02_000938"
     CDS             15348..16229
                     /locus_tag="OQN02_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1641833.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16435..17904
                     /gene="lysP"
                     /locus_tag="OQN02_000939"
     CDS             16435..17904
                     /gene="lysP"
                     /locus_tag="OQN02_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1641834.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18196..20187
                     /gene="cirA"
                     /locus_tag="OQN02_000940"
     CDS             18196..20187
                     /gene="cirA"
                     /locus_tag="OQN02_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCS1641835.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="OQN02_000941"
                     /pseudo
     CDS             complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="OQN02_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="OQN02_000942"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="OQN02_000942"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1641836.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23073
                     /locus_tag="OQN02_000943"
     CDS             21997..23073
                     /locus_tag="OQN02_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1641837.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23215..24255
                     /gene="galS"
                     /locus_tag="OQN02_000944"
     CDS             23215..24255
                     /gene="galS"
                     /locus_tag="OQN02_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCS1641838.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24535..25533
                     /gene="mglB"
                     /locus_tag="OQN02_000945"
     CDS             24535..25533
                     /gene="mglB"
                     /locus_tag="OQN02_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1641839.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25594..27114
                     /gene="mglA"
                     /locus_tag="OQN02_000946"
     CDS             25594..27114
                     /gene="mglA"
                     /locus_tag="OQN02_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1641840.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27130..28140
                     /gene="mglC"
                     /locus_tag="OQN02_000947"
     CDS             27130..28140
                     /gene="mglC"
                     /locus_tag="OQN02_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1641841.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28176..29389)
                     /gene="preA"
                     /locus_tag="OQN02_000948"
                     /pseudo
     CDS             complement(28176..29389)
                     /gene="preA"
                     /locus_tag="OQN02_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29383..30621)
                     /gene="preT"
                     /locus_tag="OQN02_000949"
     CDS             complement(29383..30621)
                     /gene="preT"
                     /locus_tag="OQN02_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCS1641842.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30815..31054)
                     /locus_tag="OQN02_000950"
     CDS             complement(30815..31054)
                     /locus_tag="OQN02_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1641843.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="OQN02_000951"
     CDS             complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="OQN02_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1641844.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="OQN02_000952"
     CDS             complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="OQN02_000952"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1641845.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32940..33635)
                     /locus_tag="OQN02_000953"
     CDS             complement(32940..33635)
                     /locus_tag="OQN02_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1641846.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33632..34030)
                     /locus_tag="OQN02_000954"
     CDS             complement(33632..34030)
                     /locus_tag="OQN02_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1641847.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34269..35216
                     /gene="dusC"
                     /locus_tag="OQN02_000955"
     CDS             34269..35216
                     /gene="dusC"
                     /locus_tag="OQN02_000955"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS1641848.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="OQN02_000956"
     CDS             complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="OQN02_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1641849.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35894..37330
                     /gene="mdtQ"
                     /locus_tag="OQN02_000957"
     CDS             35894..37330
                     /gene="mdtQ"
                     /locus_tag="OQN02_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCS1641850.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37383..38144
                     /locus_tag="OQN02_000958"
                     /pseudo
     CDS             37383..38144
                     /locus_tag="OQN02_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38261..38839)
                     /locus_tag="OQN02_000959"
     CDS             complement(38261..38839)
                     /locus_tag="OQN02_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1641851.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39009..39596
                     /locus_tag="OQN02_000960"
     CDS             39009..39596
                     /locus_tag="OQN02_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1641852.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39770..40702
                     /gene="pbpG"
                     /locus_tag="OQN02_000961"
     CDS             39770..40702
                     /gene="pbpG"
                     /locus_tag="OQN02_000961"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1641853.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40740..42455)
                     /gene="dld"
                     /locus_tag="OQN02_000962"
     CDS             complement(40740..42455)
                     /gene="dld"
                     /locus_tag="OQN02_000962"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1641854.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42651..44948
                     /gene="bglX"
                     /locus_tag="OQN02_000963"
     CDS             42651..44948
                     /gene="bglX"
                     /locus_tag="OQN02_000963"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1641855.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45200..46117
                     /gene="osmF"
                     /locus_tag="OQN02_000964"
     CDS             45200..46117
                     /gene="osmF"
                     /locus_tag="OQN02_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1641856.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46124..47281
                     /gene="yehY"
                     /locus_tag="OQN02_000965"
     CDS             46124..47281
                     /gene="yehY"
                     /locus_tag="OQN02_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1641857.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47274..48200
                     /gene="yehX"
                     /locus_tag="OQN02_000966"
     CDS             47274..48200
                     /gene="yehX"
                     /locus_tag="OQN02_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1641858.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48205..48936
                     /gene="yehW"
                     /locus_tag="OQN02_000967"
     CDS             48205..48936
                     /gene="yehW"
                     /locus_tag="OQN02_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1641859.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48917..49024)
                     /locus_tag="OQN02_000968"
     CDS             complement(48917..49024)
                     /locus_tag="OQN02_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1641860.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49084..49536)
                     /locus_tag="OQN02_000969"
                     /pseudo
     CDS             complement(49084..49536)
                     /locus_tag="OQN02_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11959 a        12199 c        13310 g        12068 t
ORIGIN      
        1 aaaacagagt ggcgataatc atcaccatcg caaaccagaa cacagcgtga taattccaga
       61 tctcggcgac gatgcccgcc actgatcctg cgatgatcca gcccacgcgc gacgtgttgg
      121 tatagagcgt ggtggctgaa cctgcctgac cgggcatcaa atcctgaaaa tagagcatcc
      181 cgatgccgcc cagaatgcca ataaaaatag catttagcag ctgcaagccc aacagaatgg
      241 caggtgaatg cgccatcagc attcctgcgt aaaaacagac gccacccacg gcagcaacgc
      301 gcattaagaa acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga
      361 tttccagccc ggcggcggtc cccatcatca caccggctag tttctcggga agatgcagtt
      421 cgttgataat aaatagcggc atgttgatga tgtacaggct gttcgagccc cacatcaatg
      481 tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc
      541 cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc atcacgccgc
      601 aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa cccatcgcta
      661 acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag
      721 agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca agggcaaaca
      781 tttgcgggtt agcggtcgag ccaaagctgc taagaaagac gccaacgaat agcaaaacaa
      841 agtagttgcg attccaagca aaaagggtgc aggccagcac gcctaacagg cagcaaaaga
      901 caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac tgacttacca
      961 gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga cgggcatgta
     1021 cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca ataccggtga
     1081 gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg
     1141 cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt ttacgctgct
     1201 tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa atgccatttc
     1261 actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg gattttccca
     1321 tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg ctcataactt
     1381 tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg tttcagcgcg
     1441 atcttgtctt taaccctaag ctaggttggc gctttttttc tcatagaggc tgaatcgttt
     1501 caattcagca agagaggaga actatgttcc agttatccgt acaggacatc catccgggcg
     1561 aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg
     1621 gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt
     1681 tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat caggtgctga
     1741 aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag
     1801 cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg ctacgccagc
     1861 tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca acaacagcag
     1921 aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag ctcgacaacg
     1981 aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg
     2041 cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc atcaatgaac
     2101 aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc aatctgcgta
     2161 gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa acggcagcca
     2221 tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag cgtctcgctg
     2281 atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca acgttgctgg
     2341 cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag tttgtagtgc
     2401 gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc attaaacaat
     2461 ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca aacggacgta
     2521 gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc tttaccgccc
     2581 agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct gctggtcttg
     2641 gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg cttatgacct
     2701 tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct
     2761 gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg gaattgatgt
     2821 caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac tgttcagcga
     2881 gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta acgttaagct
     2941 gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggtttcgaag tcacccccgc
     3001 cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg atatggtctg
     3061 tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact ggatgactcg
     3121 cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt tagtagcagg
     3181 tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa tctgggcagg
     3241 ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac gtgaacaagg
     3301 tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg
     3361 cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg caggggattc
     3421 tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac acacactgcg
     3481 tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg
     3541 tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact gacagcagga
     3601 gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca cgcgcctata
     3661 tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc
     3721 cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac agcgcgctga
     3781 acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct gagctgttcc
     3841 tgagtgaagc caaaggtcat gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg
     3901 ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac
     3961 acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga
     4021 aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag gaagtagccg
     4081 cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa tttgctggta
     4141 aaccgatgta tcgtacctct accggtctgg cgctgaagaa aacctcgcag gaactggata
     4201 aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg gcgaccactg
     4261 aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc
     4321 tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag
     4381 cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt ggttcagcct
     4441 ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc gatcgtccgg
     4501 gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct ggcttcattg
     4561 gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt acgcaactga
     4621 aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta atttccagtc
     4681 tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc attctcgaag
     4741 ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg ggggcgatcc
     4801 tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg tacgcattcg
     4861 gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg gcggcaggta
     4921 tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag
     4981 cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt tcggaaggtg
     5041 caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg
     5101 cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg cacggtggtc
     5161 tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg gtagcaatta
     5221 ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg gaagtggacg
     5281 cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc tgccctgtaa
     5341 catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga atttaaatgg
     5401 ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt tcctcaaggg
     5461 cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca caaattcctc
     5521 gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct gcccattttg
     5581 cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca tgtttaaata
     5641 tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga cagggttggt
     5701 aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt ataggttcgg
     5761 cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat tcaaatccag
     5821 gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac ctggcgttgc
     5881 tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt caatcgctgc
     5941 taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg ccgggagaaa
     6001 atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt gctataaatg
     6061 acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt gaatttattc
     6121 agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg gcatggattc
     6181 tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgcgg aaatgtgtca
     6241 aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt gaagcggaaa
     6301 ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct gcctggttcc
     6361 atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt tattacagcg
     6421 atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt aatgttaccg
     6481 gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga atgccgtttg
     6541 ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt gaatacacca
     6601 ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacaca gaatgtctga
     6661 attaaaaatc tcccctgaat tattacaaat ttctccggaa gtgcaggacg ctttaaaaaa
     6721 caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc cgttcccaca
     6781 aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg cagtacctgc
     6841 cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa aaattgaatt
     6901 actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac cttttgttgt
     6961 tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg cggcgcttgc
     7021 cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg cggaacatac
     7081 cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc
     7141 cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa ccttcggtgt
     7201 gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt
     7261 tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg
     7321 gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac agtttgcgat
     7381 gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg aagagcaggg
     7441 cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc tgaccggcgg
     7501 tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg cgagtgaaat
     7561 tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt
     7621 actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg aagggaaaat
     7681 cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa tagcatttct
     7741 gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac tgctgctgca
     7801 aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag tagaacaggc
     7861 ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg
     7921 ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta tcttcgcctt
     7981 tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt actacattgg
     8041 cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc tcaacatcag
     8101 caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg agatcccggc
     8161 gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg caatttgtag
     8221 cgggatggcg tccattgctg gttcggtgat gattggttat gccggaatgg gcgtaccaat
     8281 tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt ttgcacgtat
     8341 tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt tcagcgaaac
     8401 gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc
     8461 cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga tcaacggcat
     8521 tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa gtatttttgg
     8581 ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg ccaaccttgc
     8641 tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc tgagtttctc
     8701 cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta tatcctttgc
     8761 gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat tttcggctat
     8821 ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg cagcagcaac
     8881 gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg cgtaatgctc
     8941 gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc tattgcaaat
     9001 aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag cgccagtcct
     9061 gacaactgct ttctgttttt aattagatat cctttctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcggca aagaaatcga
     9481 tcagcgacac gcgaccattg gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcattggcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ctaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt
    12481 cactgcggca ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc
    12541 cggtaaagtg ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt
    12601 gaattctgaa acggtatcga caaacaccgg gatctcatca gcgagggtaa agacccattc
    12661 cagcgcctcg gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc
    12721 gcgcgacccg ttcagtagtt gcggtgtcaa ctgttccagc agatgggtat cgttaattgc
    12781 cagcacggtt tgatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg
    12841 caaacgaacg cagccggaga cattcacgcc ggcacggcgc gtttcttcca ggagcatttc
    12901 gccataaaag tcatcgccaa tcactgaaag caaatggacg tcacggccta acagcgccag
    12961 attgtgggcg atattgcgtc caacgccgcc tgccgaacaa tgaattgtac cgggatgaga
    13021 agccgcttgc gggtaatgga tatccgccat cccgcgaata tccatattga ttgtccccac
    13081 cactacgcag tattcctgct cggtgagaat gtaacctttg tctttaatcc ggcctttgcg
    13141 cattaaatcc ataatatgcg ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat
    13201 ttcgttctgc tgaatcagcg ggttacgccg taaaattgca aggatctcct tttcccggtt
    13261 attcatcttc aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct
    13321 gcccagatat ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgttc
    13381 tgcataatcc agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg
    13441 tcaacgcggc tgccaaaggt acttttcgca tcgttaaggt gcatcccgcg cagatactta
    13501 aagccgacaa tacgggcaaa atcagcgaac gttttttcgc attcggctgg agtatgcaaa
    13561 tcatacccgg cagcgaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct
    13621 tccacgccgt cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga
    13681 ccggcggtgt tttcaatcac cgctgttaca ccttgagttt tatccagcgc aatgttgatg
    13741 gattcggcaa tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg
    13801 tggaagttga gcaaagaaag ccccagttgt tcgcaacgct gcatttcatc tataaaggca
    13861 tcgcgcgatt tttccagagc ttcagtgacc ggatgtccga ggttgatcag ataactgtcg
    13921 tggggaagaa tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg
    13981 atggtttgcg tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca
    14041 aacgcggttg cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca
    14101 ctaacgtgcg ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac
    14161 ggtaatgata gcgggttaac aggagcaaga agtagtggtt tatgcgatga cgctttgaat
    14221 cacatagtta atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag
    14281 cagaggtttc gccccagctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc
    14341 catcgccatt gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg
    14401 taacaggtgg aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat
    14461 agtgattttg cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag
    14521 caggatgagg aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt
    14581 ttctgcatcc gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt
    14641 agaaccaatg tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat
    14701 cgccgggtag aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt
    14761 tactttactg gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca
    14821 gatactgcta ccggcaccga tcaaccaact ggtgtgctta tccagaccaa acactttctg
    14881 ccccaggaag caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc
    14941 actgataccg acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc
    15001 cagacgtaat aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg
    15061 cggatagatg gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa
    15121 cccggcaccc gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc
    15181 actcagggct aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat
    15241 attcgtcata accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata
    15301 aaattgatta tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc
    15361 tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg
    15421 tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt gactgacctg gaagggcagc
    15481 ttggcgttca actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc
    15541 tgctctatcc gcgagcgttg gcactgcttg aacaggcggt tgaaatcgaa caactgtttc
    15601 gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc
    15661 ctgcagttat cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg
    15721 ggaacagcca ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg
    15781 aaggaccgtg ccacagcact gaaatcattt ctgaaccgtg gctggaagac gagcaggtgg
    15841 ttttcgccgc gccgacttcg ccgttggctc gtggtccggt caccttagaa cagctggccg
    15901 ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg ggatattgtc gattatctgt
    15961 tgctgtcaca tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca
    16021 aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc
    16081 aattgcaggc aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt
    16141 tgtggcgtat acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact
    16201 attgcgatcc cgcaaatgtg ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt
    16261 cgatttcgtg acgcagcgcc ttcagcatgc attcgccaga aaagagattg gctgctttac
    16321 ttataatccc tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt
    16381 gccaatcgtc tgctacaatc gcgccatcat ttttaagatg gatagcattt ttgtatgggt
    16441 tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg
    16501 acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg
    16561 atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt
    16621 tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc cggttcgttt
    16681 gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac
    16741 tggtacaact gggcggtgac tattgccgtt gacctggttg cagcacagct ggtcatgagc
    16801 tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc
    16861 ctgttgaact acatctctgt tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc
    16921 aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc
    16981 aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt
    17041 ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag
    17101 ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta
    17161 cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg
    17221 attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt
    17281 ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca
    17341 gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc tactcgtatg
    17401 ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt
    17461 ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg cttcctgacc
    17521 tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt
    17581 tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg
    17641 cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc
    17701 ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa
    17761 gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt
    17821 atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag
    17881 ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag
    17941 agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt aatatttgga
    18001 ttgataattg ttatcgtttg cattatcgtt acgacacaat caaaaaaggc tgacaaatca
    18061 gaggctgttc cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg
    18121 ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt cgtagttacc
    18181 tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc
    18241 gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc
    18301 actgcatctt ccgtggaaca aaatcttaaa gatgcacccg ccagtatcag cgtcattacc
    18361 caggaagacc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct
    18421 ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac
    18481 agcagctaca ccctgattct tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc
    18541 cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg
    18601 gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc tcggcggtgt agtgaatatc
    18661 atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag
    18721 gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg accattaatt
    18781 gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg
    18841 caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc
    18901 gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac
    18961 ggtttcgacc gtcaggatcg tgattccgat tcgctggaca aaaaccgcct ggaacgccag
    19021 aactactgcg tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatcctac
    19081 ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat
    19141 acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc
    19201 ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc
    19261 aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg
    19321 ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt
    19381 ccgcgtgcct acatggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg
    19441 acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc
    19501 cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg
    19561 gagctggggc tttactacat gggtgaagaa ggctggctgg aagggggtga atccagcgtt
    19621 atcgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac
    19681 gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg acggcgcata
    19741 ccggtattta gctactacag cgttaacaaa gctcgtattc agggcgtgga aaccgaactg
    19801 aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa cgatggtcgt
    19861 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac tgctaacggt
    19921 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
    19981 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
    20041 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
    20101 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
    20161 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
    20221 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
    20281 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
    20341 cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
    20401 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactat
    20461 cccattccag catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cgggacgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatgcgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ctggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggt gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac tctggatcca gtcgcggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc
    22381 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    22441 cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg
    22501 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    22561 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    22621 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    22681 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    22741 tttcaggcga ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt
    22801 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    22861 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    22921 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    22981 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    23041 gccggaccgg caatatctaa aacatcaaga taacgatctg gattacattc gttaacaaaa
    23101 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    23161 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    23221 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc
    23281 aataacagca cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag
    23341 ctggattatc ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    23401 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    23461 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    23521 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac
    23581 tcaaaagcat tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg
    23641 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    23701 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    23761 tcttccagcc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    23821 aaagagcagg atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg
    23881 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    23941 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    24001 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    24061 ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg
    24121 gccttgcagg gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg
    24181 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    24241 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    24301 tagctatgat tgcaccgttt taatgttgta acccgtatgt aacagtgaat aatcactttc
    24361 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    24421 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    24481 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    24541 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    24601 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    24661 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    24721 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    24781 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    24841 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    24901 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    24961 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    25021 cagattcagt tcgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc
    25081 acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat
    25141 accgcaatgt gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    25201 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    25261 gaagcactga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    25321 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac
    25381 aaccaggcga aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct
    25441 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    25501 aaagacaact tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    25561 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    25621 ggggaatact tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt
    25681 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt
    25741 gcaggaaaat cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    25801 attttattcc agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt
    25861 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    25921 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    25981 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    26041 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    26101 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    26161 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg
    26221 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    26281 accgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    26341 cttaatcagc gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt
    26401 aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    26461 gagatcctcg gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    26521 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac
    26581 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca
    26641 acgggaattt atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac
    26701 tacaaaaata aagttggttt actggataac tcgcggatga aaagcgatac ccagtgggtg
    26761 attgattcga tgcgggtaaa aacgccgggt catcggacgc aaattggttc gctctccggt
    26821 ggtaatcagc aaaaggtgat tattggtcgc tggctactaa cgcaaccaga aatattaatg
    26881 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgaaattta tcagttaatt
    26941 gccgaactgg cgaagaaagg caaggggatt attattatct cctctgaaat gcctgagttg
    27001 ttagggataa cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgat
    27061 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    27121 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    27181 atttacgtcg ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta
    27241 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc
    27301 ggtgtggcag ggttaattgt cacccagggg accgatcttt ctgctggtcg tcaggtaggg
    27361 ctggcggcag tggtggctgc gacattattg cagtccatgg ataacgccaa caaagtgttc
    27421 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    27481 gtgatcggtt tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc
    27541 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    27601 gcgtcgccaa tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    27661 ctggggagtt tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc
    27721 tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg
    27781 gaagcggcaa aaatatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    27841 tctggcgtgt tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    27901 aacaacctcg gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta
    27961 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    28021 gtcatcaact atggcctgac gtatatcggc gtaaacccat actggcagta catcatcaaa
    28081 ggggcgatta ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaagtga
    28141 tgtgattgcg gatattgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    28201 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    28261 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    28321 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    28381 catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    28441 acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg
    28501 agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta ctgcataatg
    28561 ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc atcttcccag
    28621 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatt
    28681 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    28741 gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    28801 ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    28861 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    28921 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    28981 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagctgct
    29041 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    29101 ccttcagccg acgcagggcg gtcagattct cttccaacgg atgttcagca atctgctcca
    29161 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    29221 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    29281 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    29341 tgggaaagtt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    29401 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    29461 ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc gggtctgata
    29521 attttgtgtt ttgatggtgt tacgctgctg ctctaactcc gcaaaggcat ccagtctggc
    29581 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ctgataaccg
    29641 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    29701 cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa actcatctaa
    29761 ctcttcacgt gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    29821 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    29881 ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa
    29941 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttccgc
    30001 tttcagttgc tccagggtga gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc
    30061 cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc ggaattgcgg
    30121 aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg tgacgtcata
    30181 accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc caataatcgc
    30241 gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt gttgttcaaa
    30301 atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac cggcacgggt
    30361 acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    30421 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    30481 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt cgtgacataa
    30541 taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag tgagttcatc
    30601 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc
    30661 taaataaata ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta agctgtgaaa
    30721 ggattcacaa atagctgcgg ttcagaatgt tatatccgca gcttattgag tgaagtaata
    30781 attattctca ataattaaca gaaaagagaa aatattaacg atagaaaata agataaacaa
    30841 tcatgccgat agataaaatt ccgaagccaa tatatgccac catataagcg aagaagagtc
    30901 cgggattttt tgctcgccag acatacaccg gttccggtgc atttttaacc cgtttatcaa
    30961 tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa
    31021 aaacggcaac gacaaaaaac tgctgtacat ccataactac tttccttgtt tcttttgtaa
    31081 ttcaagtaac tgttcgggtg taacggcggg atacccctgc gcatcttccg gcacctggtg
    31141 catagccgga atagggacca gcggccctaa aaaacgcggt tcacgtttaa aaatataaag
    31201 gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt
    31261 cggtgacggt acggcataac actgagcctg aatccccata tgcagcgcaa taaataatgc
    31321 tcgctcacag tggaaacgtt gggtgataat aatgaaatca ttagtatcga aaactttgcg
    31381 tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg
    31441 gtcgacacca gcagcgatta aatctttgcg catggtcatc ggctcgttat aactttgcaa
    31501 tgcgttatcg ccgctcagta atagataatt taccttaccg ctgttatagg cattaatcgc
    31561 tccttgaatg cggtagcgat aatactgatt aataacgccg gtacgataat attttgctgt
    31621 gccgagcacc acgccgacct ggcggtaggg gagatcctgc aattcgtcgt agatataagg
    31681 cgcggttttc cagctcatcc agcgatcgag gccgagcaca gtcaacagca gcaagccgat
    31741 caggactaac aggctgagga acacgcgctt taacatgcag gagactcgag ctaaggaaaa
    31801 tttccatcag gctactttac ccgctggttt tgcgcaagaa acggcaaact gtttgccggg
    31861 aatttcgaca ccaggagagg gctggcaagc cggacaaggc gttcacgccg catccggcac
    31921 caggcttaag cgagaagcac gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg
    31981 gaggtggcat cccactgaat caacggcgca tcggcttttt ctgccagaac cgcgcgctgg
    32041 atatccgggt aatcataacc cttgagattc aacagaatta acgccccctg caacggcggc
    32101 agagtcgggt tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg accgtcttta
    32161 cattccagcg cgacaccgct tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct
    32221 gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc
    32281 atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggcag atagtcacgc
    32341 agcgcgtgtg cctcgcggcc cggcaaatga atacgcagat ccagaccgct gttcagttca
    32401 ttcataaact gacggcagtg accacaaggc gtatagttaa cggtgatggc tgcaagcgct
    32461 ttttcaccac tcaaccaagc gtggctgatt gcgctttgtt cggcatgaac ggtttgctgc
    32521 attgttgcac caataaattc catattggca ccaaaatacc aggttccgct cacaccgcgc
    32581 gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc cgccagcgga
    32641 agtagtgcga atgccagcgc gtcttcgtcc agtcccgttg cgctcttcag cgatgagact
    32701 tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc cagtgcagat
    32761 tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct
    32821 cataatcatg taatgggata acagtttacg aacccgttat ggctttatat gtgatctgaa
    32881 tctcattaat tatgcaacac gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt
    32941 tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga agtaattatc
    33001 ccgcataaca ccagcgctag cgaactaaat gcaccttcct gataatccag ctcggcgcag
    33061 cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc agctttggta
    33121 cgaatacgca tcgcattaag caatgtatgg ccaaataccg cgccgaggat gccgacgaaa
    33181 atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac cgccattgca
    33241 ataggcgtgg tgactgattt cggcaggatt gacgcggcga tttccggtga agcgcccatc
    33301 aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa acagatggtg
    33361 atgatcgatt tccagcgcgc gcggatctgg tgtagctgct catataaagg ataggccagc
    33421 gcgacgaccg ccggttgcag cagatcgttt agcacttcgc tacctttaaa gtaactgtcg
    33481 taagagatgc cggtcagcat taaaaaagga atgatcacca ccattgctac cagcaacggg
    33541 ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac
    33601 gtcagcggta atgaccacca gatattcgcc atcattcttc tgatcctttc tgacctacga
    33661 ctttacgttc accgtgtacc agttgtgaac tccagctcac caccagaaaa accaccagcg
    33721 tactgacagc acaggaaacc actaccgggc caaactgcgc gcggagcaaa tcaaaatatt
    33781 gcatgacgcc tacgccaatc ggcacaaaca atagcgccat atagcgaatc agtacgtagc
    33841 accccggatt gacccatttc gccggaagaa tttgcaaggc cagcaggaca aacaggatct
    33901 gcatcccgat gatgctgccc ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg
    33961 catacaggca ggcataaatc aggatgaaag cgcgtaaata ttgccagata atgttcagtg
    34021 tcttactcat aatcaacacc ccagatgaga tggattcatc atacaattaa ttcgtaaaac
    34081 gtgctaccga tcacattaag aaatcagcgc ttattttttc gctacccggt attacgcaat
    34141 gagcagcgaa ctcaggcata catcaagcat agagagggat tgcagtttgc agcaatccct
    34201 ctttgcagac ctttaatccg ctgaccatag gcgctaacat agcgcctcat ttttttgcgg
    34261 gtgatgatat gcgtgtgtta ctggcaccga tggagggagt gcttgactct caggtgcgtg
    34321 aattgctgac cgaagttaac gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg
    34381 atcaactgct gccggtaaaa gtctttcatc gcatttgccc tgagctacaa aacgccagcc
    34441 ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg tcagttccca caatggctgg
    34501 cagagaacgc cgcccgtgcg gtggagttag gtttctgggg cgtggatctc aattgcggct
    34561 gcccgtcgaa aacggttaac ggtagcggcg gcggggcgac gttactcaaa gatcctgaac
    34621 tcatctacca gggggcaaaa gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg
    34681 tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc
    34741 aacaggctgg cgctacggag ctggtggtgc atgggcggac gaaagagcag ggttaccgcg
    34801 cggagcatat tgactggcag gcgattggcg agattcgcca gcggctgaat attccggtga
    34861 ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca atgcatggcg atcagcgact
    34921 gcgacgcagt gatgattggt cgcggggcgt tcaatattcc caacctgagc cgggtggtaa
    34981 aatataacga accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa aaatataccc
    35041 gtctggaaaa gcagggcgat accgggttat atcacgttgc gcggattaaa cagtggttga
    35101 gttatttgcg taaagaatac gatgaagcaa cggaattatt tcagcatgtt cgggtgttga
    35161 ataactcccc tgatattgca agggctattc aggcaattga tatcgagaaa ctctaaaaac
    35221 catagtgatg tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt
    35281 ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg atgtgggctg
    35341 agggcgcgtt ttacagggat gttacctcgc gcccgacccg gtagccgtaa gggataagcc
    35401 gagggcaccg cgcagcggcg attttgttcg ccagagcccg ggggggcagg gggcggcggc
    35461 gattggtcgc cccctgcgcg ctccttgcgc cagtgattat atgaagcaat gcacatgaca
    35521 ggagcgcaac aggatgagag actgaaatat tctcgttatt ttcggatctg atgacattaa
    35581 ttaattttaa aatatcatct taaatacgcc agtcaccacc agtagcccaa tcaggaaaat
    35641 aatcaataca gcccagagta taattttcat tggcgttcct ttatttgtga gtgctgaata
    35701 agtataggaa aaaacgaagt cctttgccgt tatccataaa cgattaagat aaatcggaag
    35761 atatcattta cttagtctat atttttctgg gtatgtcgtt ttattgctac tgatgtattg
    35821 ccacgcgaaa ctaaaaaata tatcgcaaca cacggtaatc tccgcgcacc gtttcttacc
    35881 tgattattcc cccatgaatc gtgattcctt ttatccagcc atcgcctgtt ttccgctgtt
    35941 actgatgctg gccgggtgtg cgcctatgca tgaaacccgc caggcgttaa gccagcaaac
    36001 gcccgctgca caagttgaca ccgcattacc cacggcgctg aaaaatggtt ggccagacag
    36061 ccaatggtgg ctggagtatc acgataatca gctcacttca ttaattaaca atgcactaca
    36121 gaacgcaccg gatatgcagg tggctgaaca gcgcattcag ctagctgaag cccaggcgaa
    36181 ggcagttgcc actcaggatg gtccgcagat cgacttttcg gcggatatgg agcggcaaaa
    36241 aatgtcggca gaagggctaa tggggccgtt tgctctgaac gatccggccg caggtacgac
    36301 cggtccgtgg tacaccaacg gaacttttgg cttaacggcg ggctggcatc tcgatatctg
    36361 gggaaagaat cgggcggagg ttactgcccg cctgggtacg gttaaagcac gggcggcgga
    36421 acgcgagcaa acccgccaat tgctggctgg cagcgtagcc cgcctgtact gggagtggca
    36481 aacccaggcg gcgttaaaca cggtcttgca gcaaatagaa aaagagcaga acaccattat
    36541 cgcgaccgat cgccagctat atcagaacgg gattacttct tcagttgacg gtgtggaaac
    36601 cgatattaat gccagcaaaa cccggcagca gctcaacgat gtcgcgggga aaatgaaaat
    36661 tattgaggca cggttaaatg cactcacaaa tcaccagact aagtcattga agcttaaacc
    36721 ggtcgcgttg ccgaaagtgg caagccagct tcctgatgaa ctggggtact ccttactggc
    36781 ccggcgggca gatttgcagg cggcgcactg gtacgttgag tcatcgttaa gcactattga
    36841 tgcggcaaaa gcggcatttt atcctgacat caacctgatg gccttcctgc aacaggatgc
    36901 gttgcactta agcgatctgt tccgtcattc cgcgcagcaa atgggcgtta cggcaggcct
    36961 gacgctacct attttcgata gtggtcgtct taacgccaat ctcgatatcg caaaagccga
    37021 aagcaacttg tctatcgcca gctacaacaa agcggtggtt gaagcagtga atgacgtggc
    37081 gcgggcagcc agtcaggttc agacactggc ggagaaaaac cagcatcagg cgcaaattga
    37141 gcgcgatgcc ttgcgtgtgg taggtcttgc gcaggcccgc tttaacgcgg gcatcattgc
    37201 tggttcccgc gtcagcgaag ccagaatccc ggcgctgcgt gagcgggcca atggcctgtt
    37261 attgcaaggg cagtggctgg atgcctccat tcgactcacc ggtgcgctgg gcgggggtta
    37321 caaacgctga tgatatgcgc cttctatact taacgtttat tcagcgttaa gtggagaact
    37381 cgatggcata ggtcgcgata attaccgctt ccgattcggg gatcggcaaa gagtgcgcgt
    37441 tattactggc gcagcagggg tttgatattg gcattacctg gcactcagat gaagaagggg
    37501 caaaagatac cgcgcgtgag gtggttaccc acggcgtacg tgcggagatc gtgcaactgg
    37561 atctcggcaa tctgccagaa ggtgcgcagg cgctggagac actcatccag cgatttgggc
    37621 gtattgatgt gctggtgaat aatgcgggtg caatgaccaa agcgccgttt cttgatatgg
    37681 cttttgatga gtggcgcaag atttttaccg ttgatgttga tggtgcattt ttatgctcgc
    37741 aaattgcggc tcgtcagatg gtgaaataag ggcagggcgg tcgcatcatc aacattacgt
    37801 cggtacatga acatacgccg ctgccggatg ccagcgccta cacagccgct aaacatgcgc
    37861 tcggcgggtt aaccaaaacg atggcgctgg agttggtcag gcataagatt ttggtgaacg
    37921 cagtcgcgcc tggggcgata gccacgccaa tgaacagcat ggatgatagc gacgtgaagc
    37981 ccgacgcgga gccttcgatt cctctacggc gttttggcgc aacgcatgag attgccagcc
    38041 tggtggtgtg gctttgttcg gagggcgcaa attacacgac agggcagtcg ttgatagtgg
    38101 atggcggctt tatgttggcg aatccgcagt tcaacccaga atagcgcagt gaggtgcgtg
    38161 gatatttcaa tgggttaggc cagataagac accagcgtcg catcaggcat ggaacacccg
    38221 gtgccggatg tggagtgaat gcttatctgt ggcaactgct ttacgcctga ttatccggct
    38281 ttttcttccc gcgccgtttc agccaccagc gcacgcccac caccagaacc acgaccagaa
    38341 tcaaccagac ccagtgcttc aaatgctggt cgagattgtg caaccacggc gcaatcacct
    38401 gaccaccagc gtaaccaata gtggtaaaaa tcaacgccca ggcaaatgcg ccgagaatat
    38461 tcagcggcag aaagattttc ggcggcagct ggctggcacc aatcagcgtc gggccaatca
    38521 cccgaaagcc atacataaag cgcgtaccaa tgacaaacag atacggatgg cgttggataa
    38581 gtttctgtgc ccgctcaatt ttatcctgat gtttcgagaa acggcgtaac agcttgccgc
    38641 caaaccgccg cccgcacaga tagagcacct ggtcgccaat catgccacca agcgccacag
    38701 aaagtaccac cagtgggaac tttaatagcc cctgatgtgc cgcaacgcct cccagcaaag
    38761 tcacggtttc accttccgcc aggctaccga taaccagcgc ggcataacca tattgtgaga
    38821 tgagtgtatt gagagccata ggccagtttg tctccctttg gttgcctgta agcatacacc
    38881 ctgtgaatca ttcacgctga aaaaggacat acatcagacg gtgcaaatta catggatgaa
    38941 caaaaaataa tcacaagctg gcttatactt gaactgtcag gttcatgaac tcacaacaag
    39001 ggggcggtat gagccatgtc tggggactgt tttcccatcc cgatcgtgaa atgcaggtga
    39061 ttaatcgcga aaacgaaacg atttctcacc attataccca ccacgttttg ctgatggcgg
    39121 cgattccggt gatttgcgcc ttcattggca ctacacagat tggctggaat tttggcgatg
    39181 gcactatcct gaagttatcc tggtttaccg gactggcgct ggctgtcttg ttttacggcg
    39241 ttatgctggc tggggttgcg gtgatggggc gggtcatctg gtggatggcg cgcaattacc
    39301 cgcagcgtcc gtctcttgcg cactgcatgg tcttcgcggg ctacgttgca actccgctgt
    39361 ttttaagcgg tctggtggcg ctttatccac tggtctggtt gtgtgcgctg gtcggcacgg
    39421 tggcgctgtt ttacaccggc tacctgcttt accttggtat tccgagcttc ctgaatatca
    39481 acaaagagga aggccttagc ttctccagtt cgacgctggc gattggcgtg ctggtactgg
    39541 aagtgctgct caccctaacc gttatactat ggggctacgg ttatcgtttg ttctagaaag
    39601 ttctttgacg ttgcattgct ggcgtaaatg tcagcaatgc agcatttact cacgattctc
    39661 ctttgacgat cgcacttttt gctcgttatg atggcgtcgc tagcctcagt aaatccttac
    39721 tccggcggtg cgcaacccgt gcgcgtgaac cactatctga atgctcatca tgccgaaatt
    39781 tcgagtttct ttatttagct tggccctgat gctggctgtg ccttttgcac cgcaggccgt
    39841 tgcgaaaacg gcagccgcta ccaccgcttc acaaccggaa attgcctccg gtagcgcgat
    39901 gattgttgat ctgaatacca acaaagtgat ctattcgaac cacccggatc tggtgcgtcc
    39961 gattgcgtct atcagcaaat taatgaccgc gatggttgtg ctggatgcac gactgccgct
    40021 ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag atgaaagggg tctattcgcg
    40081 cgtacgactg aatagcgaaa tcagccgtaa agatatgctg ttgctggcgc tgatgtcttc
    40141 agaaaaccgc gcggcggcaa gccttgcgca ccattatccc ggtggttaca aagcctttat
    40201 taaggcaatg aatgcgaaag cgaaatcgct cggaatgaac aacacgcgct ttgttgaacc
    40261 taccggattg tcggtgcata acgtttcaac tgcccgtgac ttaaccaaac tgctcattgc
    40321 cagcaaacaa tatccgttga tcgggcagtt aagtactacc cgggaagata tggcaacctt
    40381 ctctaacccg acgtatacgc tgccgttccg caatactaat catctggtgt atcgcgataa
    40441 ctggaatatt cagttaacca aaaccggctt taccaatgcg gcgggccatt gtctggtgat
    40501 gcgtacggtt atcaataata aaccggtggc gctggtagtg atggacgcgt ttggcaaata
    40561 tacccatttt gccgatgcca gccgcctgcg tacctggatt gaaaccggta aagtgatgcc
    40621 tgtgccggca gcagcgttga gctataaaaa acaaaaagcc gcccaaatgg cggcggcggg
    40681 gcagacggca cagaacgatt aagtgaattc ggatggcgat actctgccat ccgtaatttt
    40741 tactccactt cctgccagtt tttccgttta ctggttttac cgatccccgg attcatgcta
    40801 ttggtcggat cattctcgcg atagaacttc tgcaacgtct ccggtgcttt atacaaatga
    40861 ccgacgttat gctcggcagg gtactgcgcg ccgcgctgct gtagcagttc cagcatctgt
    40921 tcttttaacg catgcacatc cacgcctttt ttcactatgt aatcctgatg gaagacatag
    40981 cacataaaat ggccgtaata gagcttgtgc accagctggc tgtcgatctc cggcggtaaa
    41041 tgctcatacc actcggtgtc gttacgccgc agagcgatat ccagtgccag aatgtcttcg
    41101 acttcatcgg aatgcaccgc ctgataacga attgctgcgc ccgcagcggc gaaacggtgt
    41161 aaaaacgctt tgctaccttc ctccggcgta cagacaaaga aatcgccctc ggcctgtttg
    41221 aaataatcca ccagccacga tttggcttca ccgacgccat cgcctgccat ttttaacagc
    41281 agatgatgct cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg gctggggaac
    41341 aggtgaccga atttttgcat tgcacggtca gtaaaatgcg gacggaagaa tttcactttc
    41401 tccagcatcg catcggtgcg tcccttgaga ttaaagaaga acggcatctt gtcggtgcct
    41461 aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt ccgcaatatc gtagatatcc
    41521 cggtgcatat attccccggc aaccggcaga ttttcgaagt tagccagaat atgacggcgg
    41581 atttcggtca gcacttccgg ctggttggtg ccgatataaa acacctgctg atttttttcc
    41641 gcttcgaagg tatcaagacg taccgcaaag acggccagct tcccggcgca accagaagat
    41701 tcaaataacc gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc aatatcacga
    41761 acgcggtgga catagtcata atcgtgggcg tgacgaccat cgtgacgcac atcgtcatct
    41821 ttgatgcgat cgtcatccag cttgctaagg atctgctccg gcgtttcgcc cagatcaatc
    41881 cccagatggt tcaccagcgt cagtttgcca tcttcatcta tacgcgcgaa taacgacatt
    41941 tcggtatacg ccgggccacg ttgcaccagc gagccgccgg agttgttaca aataccgccg
    42001 atgaccgatg cgcctataca cgatgatcca atcaccgaat gcggttcgcg tcccagcggt
    42061 ttgagggctt tttccagcga atagagcgtg gtgcccggat aggccagcac ctgttcgccc
    42121 ttgccgagaa cgtgcagctt gtcgagacgc agggtgctga tgatcacgat atcgcgatca
    42181 tagtcgttac cgtttggcgt cgatccttcg gtcaggcctg tattggcagc ctgcatcaga
    42241 ataattttgt cggcggtgac gcaggctttc agcacccgcc acaattccag tagtgagcca
    42301 gggaaaacga cagccagcgc gtcgccctga ccagaacgga agcccttgcg atagcgggcc
    42361 gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca gacgggcaag ttcattcaaa
    42421 aaggctttat tatcagttgt tgtcatggaa gacattttcc actccttgtg gtggcgaaaa
    42481 aatatcacaa actagaatag cgccttgtat gacaattcag cgagtgaata ttttgaaaat
    42541 tcagacaata aactgagcat tctttttctc tctccatcat gcttatggca aactacggtt
    42601 tttgcaccat tttcgctgcc acgtcgggca acaaaggaag aaaaatccat atgaaatggc
    42661 tatgttcagt aggaatcgcg gtgagtctgg tcctgcagcc agcactggcg gatgatttat
    42721 tcggtaacca tccattaacg cctgaagcgc gggatgcgtt cgtcaccgat ttgctcaaga
    42781 aaatgacggt tgatgagaaa attggtcagc tgcgcttaat cagcgtaggc ccggataatc
    42841 cgaaagaggc gatccgcgag atgatcaaag acggtcaggt tggggcgatt ttcaacaccg
    42901 taacccgtca ggatatccgc gccatgcagg atcaggtgat ggaattaagc cgcctgaaaa
    42961 ttcctctttt ctttgcttac gacgtgctgc acggtcagcg cacggtgttc ccgattagcc
    43021 tcggtctggc ctcgtctttt aacctcgatg cggtgaaaac ggtcgggcgt gtctctgctt
    43081 atgaagcggc agatgatggc ctgaatatga cctgggcacc gatggtcgat gtctcgcgcg
    43141 atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc acctcaataa
    43201 tgggtaaaac catggtggaa gcgatgcagg gtaaaagccc ggcagatcgt tactcggtga
    43261 tgaccagcgt caaacacttt gccgcatacg gcgcggtaga aggcggtaaa gagtacaaca
    43321 ccgtcgatat gagtccgcag cgcctgttta atgattatat gccgccgtac aaagcggggc
    43381 tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc gctgaacggc acgccagcca
    43441 cctccgattc ctggctgctg aaagatgttc tgcgcgacca gtggggcttt aaaggcatca
    43501 ccgtttccga tcacggtgca atcaaagagc tgattaaaca tggcacggcg gcagacccgg
    43561 aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat gagtatgagc gacgagtact
    43621 actcgaagta tctgcctggg ttgattaaat ccggcaaagt gaccatggca gagctggacg
    43681 atgctgcccg ccatgtactg aacgttaaat atgatatggg gttgtttaac gacccataca
    43741 gccatttggg gccgaaagag tctgacccgg tggataccaa tgccgaaagc cgcctgcacc
    43801 gtaaagaagc gcgtgaagtg gcgcgcgaaa gcctggtgtt gctgaaaaac cgtctcgaaa
    43861 cgttaccgct gaaaaaatcg gccaccattg cggtggttgg gccactggcg gacagtaaac
    43921 gtgacgtgat gggcagctgg tccgcagccg gtgttgccga tcaatccgtg accgtactga
    43981 ccgggattaa aaattccgtc ggtgaaaacg gtaaagtgct gtatgccaaa ggggcgaacg
    44041 ttaccagtga caaaggcatt atcgatttcc tgaatcagta tgaagaagcg gtcaaagtcg
    44101 atccgcgttc gccgcaagag atgattgatg aagcggtgca gacggcgaaa caatctgatg
    44161 tggtggtggc tgtagtcggt gaagcacagg ggatggcgca cgaagcctcc agccggaccg
    44221 atatcactat tccgcaaggc caacgtgact tgattgcggc gctgaaagcc accggtaaac
    44281 cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa gatcagcagg
    44341 ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg cggtaatgca attgccgatg
    44401 tattgtttgg cgattacaac ccgtccggca agctgccaat ttccttcccg cgttctgtcg
    44461 ggcagatccc ggtgtactac agccatctga acactggtcg tccgtataat gccgacaagc
    44521 cgaacaaata cacttcgcgt tattttgatg aagctaacgg agcgctttat ccgttcggct
    44581 atggtctgag ctataccact ttcaccgtct ctgatgtgaa actttctgcg ccgaccatga
    44641 agcgtgacgg caaagtgacg gccagcgtgc aggtgacgaa caccggtaag cgcgaggggg
    44701 ccacggtagt gcagatgtac ttgcaggatg tgacggcttc catgagtcgc cctgtgaaac
    44761 agctgaaagg ctttgagaaa atcaccctga aaccgggcga aactcagact gtcagcttcc
    44821 cgatcgatat tgaggcgctg aagttctgga atcaacagat gaaatatgac gccgagcctg
    44881 gcaagttcaa tgtatttatc ggcactgatt ccgcacgcgt taagaaaggc gagtttgagt
    44941 tgctgtaatg ctttagtaag ggcgcgacgt ttgcgccctt tgcctgatgt tcatatctta
    45001 acttgctgaa ttttcgccct ttgtaggccg gataaggcgc tcgcgccgca tccggcagga
    45061 aacgccggac cgaatatgcc tcttaagtat ttatctgatt ctgcaactga atccttccgc
    45121 tcaggctaac tctgtcatta tcaactatgc ttttctctta attcgctgaa aacaacagta
    45181 aaagaatgag gaaagcatca tgccactctt aaagctctgg gcaggttcac tggttatgtt
    45241 ggcagccgtg agcctgccgc tacaggcggc ttcccccgtt aaagtcggtt caaaaatcga
    45301 taccgaaggc gcgctgctcg gcaacatcat ctttcaggta ctcgaaagcc acggcgtacc
    45361 gacggtgaat aaagtgcaac tcggaacgac tcctgtgggg cgcggggcga ttacttccgg
    45421 tgaactggat atctatccgg aatataccgg caatggcgct ttcttcttta aagatgaaaa
    45481 cgatgcagcg tggaaaaatg ccgggcaggg ctacgagaaa gtcaaaaagc tcgatgcgga
    45541 gcacaacaag ttaatctggc tgacgcccgc gcctgcaaat aacacctgga ctatcgccgt
    45601 gcgtcaggat gtggcagaga aaaacaaact cacttcgctt gccgacctga gtcgttatct
    45661 gcaagagggc ggcaccttca aactggcagc ctcggcagag tttatcgaac gcgccgatgc
    45721 gttacccacg tttgaaaaag cctatggctt taagctcggt caggatcagt tgctgtcact
    45781 ggctggtggc gacacggcgg tgacgattaa agccgctgcc cagcaaacct ctggcgttaa
    45841 tgccgcaatg gcttacggca ctgacggtcc ggtcgcggcg ctggggctgc aaaccttaag
    45901 cgatccgcaa ggcgtgcaac ctatctacgc gcctgcacca gtggtgcgtg aatcggtgtt
    45961 gaaggagtat ccgcaaatgg cacagtggct acagccagtc ttcgccagcc tcgatgcaaa
    46021 aacattgcag caactgaatg ccagcattgc agtggaagga ctggatgcca aaaaagtggc
    46081 tgccgactac ctgaaacaaa aagggtgggc gaagtaattt cccgtgactt atctacgtat
    46141 taatcctgtt ctggcgctgc tgttgttgct gacggcaatc gcagcggcgc tgccgtttat
    46201 cagttacgcg cctaatcgtt tagtctcggg tgaagggctt catctctggc aactgtggcc
    46261 gcaaacgatc tggatgctgg tgggcgttgg ttgcgcctgg ctgacagcct gttttgtccc
    46321 cgctaaaaaa ggcagcattt ttgcactcat tctggcgcaa ttcgtcttcg tattgttggt
    46381 gtggggagct ggaaaggcgg cgacccaact ggcgcaaaat ggcagtgcgc tggcgcgtac
    46441 cagcctcggc agaggtttct ggctggctgc ggcgctggca ttgctggcct gtagcgatgc
    46501 catccgccga atctccacgc atccgctgtg gcgctggttg ttgcatatgc agattgccat
    46561 tattccgctg tggttgctgt actccggcac gcttaacgat ctctcactaa tgaaagaata
    46621 cgccaaccgt caggatgtgt ttgacgacgc gctggcacaa catctgacgt tgctgtttgg
    46681 tgcggtgctg cctgcgttag tgattggtgt gccgttgggc atctggtgct acttttccac
    46741 tgcgcggcag ggggcaattt tttctctgct caatgtcatt cagaccgtgc cttcggtggc
    46801 gctctttggc ctgttgattg cgccgcttgc cgcgctggtt acggcctttc cgtggctggg
    46861 gaagctcggc atagcaggaa ccggaatgac acccgcactg attgcgctgg tgctctatgc
    46921 cttgctgccg ctggtgcgag gcgtggtagt cggtttgaac cagatcccgc gcgatgtgct
    46981 ggagagcgcc agagcgatgg ggatgagcgg ggcgcagcgt ttcctgcatg ttcagttacc
    47041 gctggcgtta ctggtatttc tgcgcagcct gcgggtggtg atggtgcaaa ctgtaggtat
    47101 ggcggtgatt gcggcgttaa tcggcgcagg cggttttggt gcgctggttt tccaggggct
    47161 gctaagcagc gccattgatt tagtgttgct gggggtgatc ccggtaattg ttctggcggt
    47221 gcttaccgac gcgctgttcg atttgcttat cgcactgctg aaggtgaaac gtaatgattg
    47281 aatttagcca tgtcagcaaa ctgttcggcg cacaaaaagc cgttaacgat ctcaatctca
    47341 attttcagga agggagtttt tcggtgctga ttggcacatc tggctccggc aaatccacca
    47401 ccctgaaaat gattaaccgc ctggtggagc atgacagcgg agtgatccgc tttgccggag
    47461 aagaaattcg ctcgctgcca gtactggagt tgcgccgccg gatgggctat gccattcaat
    47521 ctattggcct gttcccccac tggagcgtgg cgcaaaacat cgctaccgtg ccgcaattac
    47581 aaaaatggtc gcgggcgcgg attgacgatc gtatcgacga attaatggcg ctactggggc
    47641 tggagtcaaa tttgcgtgag cgttatccgc atcagctttc cggtggtcag cagcaacgtg
    47701 tgggagtggc gcgtgcactg gctgccgatc cgcaagtctt actgatggat gaaccttttg
    47761 gcgcactgga cccggtaacg cgcggcgcgt tgcaacaaga gatgacgcgc attcaccgtt
    47821 tgctggggcg cactattgtg ctggtcactc atgatattga tgaggcgcta cggctggcag
    47881 aacatctggt attgatggat cacggtgaag tggtgcagca ggggaatccg ctgacgatgc
    47941 tgactcgtcc ggcgaatgat tttgtccgcc agttttttgg acgtagtgaa ctgggggtgc
    48001 acctgctttc gttacgtagc gtggcggatt acgtgcgtcg cgaagaacgg gcagaaggtg
    48061 aggcactggc agaagagatg acgctacgcg atgcgctctc cctgtttgtc gcgcggggat
    48121 gcgaggtgct gccggtggtg aacacgcagg gccagccttg cggcacgctg cattttcagg
    48181 atctgctggt ggaggcgtaa gcgtatgaag atgttgcgcg atccgctgtt ctggctcatt
    48241 gctctttttg tggcgctgat tttctggctg ccttacagcc agccgctgtt tgctgccttg
    48301 ttcccacaac tgccacgacc cgtttatcag caagaaagtt ttgcagctct ggcactggct
    48361 catttctggc tggtgggaat ttcgagtttg tttgcggtga tcattggcac aggtgccgga
    48421 attgctgtta ctcgcccgtg gggcgcggaa tttcgcccac tggtggaaac tattgccgcc
    48481 gttggacaga cttttccgcc tgtcgcagtg ctggcgattg ccgttccggt gatcggcttt
    48541 ggtctgcaac cagcgattat cgccttgatc ctttacggtg tgctgcccat cctgcaggtg
    48601 acacttgccg ggctgggagc gattgatccc agcgtgacag aagttgcgaa aggtatggga
    48661 atgagtcgag gtcagcgact gcgtaaggtc gagctaccgc tggcggctcc ggtgattctg
    48721 gcgggcgtgc gaacttcggt gattatcaac attggtacgg cgacgatcgc ctcaacggta
    48781 ggggccagta cgctgggtac gcccatcatc atcgggctta gcggatttaa taccgcgtat
    48841 gtgatccagg gggcgttact ggtggcactg gcggcgatca tcgcagaccg cctgtttgaa
    48901 aggctggtgc aggcgcttag ccagcacgca aaataaaggt ataacctgcg agcatgacgc
    48961 caccaattcc gcctaacgcc ataaacagga acagggcgat gaccccaatt ttagctatgc
    49021 gcataatgca ctccttatgt taacgaaagg attgtacagt aaagcgcatt tgttaacgaa
    49081 tcattaaata ccgagtggga aaatatcatg gccttgttct tgccaactgg tgagttgctg
    49141 ctgttgggcg gaggttcgat tttcaccgca ccacaccagc aatgtacggc cttcgaatag
    49201 ttcagggcgt agttgattga gcgagtgggc gagcgaggac atcaatgcgc catccttgtt
    49261 gactggcaat ccagccctcc agccacagac gggtggtatc ctgaatattc cagccaacca
    49321 ccagcgcatc tttaccctgt tttttacgtg ccgaagccag acaaatggcg atgtagttga
    49381 tcagtacgcc gtcgaggatc gccagcagcg cctggagagt cggttgttgg cactgaagcc
    49441 gtcggcgcag aggaataaac agatgtgtgg tgagtgtctg ggcggggtaa tcctgaccgc
    49501 gctctttgat ccacgttcgc aggctatgta gattgc
//
LOCUS       DAJVRL010000017        48553 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVRL010000017 DAJVRL010000000
VERSION     DAJVRL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.017"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..1227
                     /locus_tag="OQN02_000970"
     CDS             <1..1227
                     /locus_tag="OQN02_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1641861.1"
                     /translation="EFELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLEN
                     HPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYM
                     IIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGN
                     IALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELV
                     YVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDK
                     NFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPE
                     TVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAE
                     KYLLAQFGVDIGEAAQ"
     gene            1224..2189
                     /gene="yggF"
                     /locus_tag="OQN02_000971"
     CDS             1224..2189
                     /gene="yggF"
                     /locus_tag="OQN02_000971"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1641862.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2211..2720
                     /gene="fumE"
                     /locus_tag="OQN02_000972"
     CDS             2211..2720
                     /gene="fumE"
                     /locus_tag="OQN02_000972"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCS1641863.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2717..3430
                     /locus_tag="OQN02_000973"
     CDS             2717..3430
                     /locus_tag="OQN02_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1641864.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3402..4084)
                     /locus_tag="OQN02_000974"
                     /pseudo
     CDS             complement(3402..4084)
                     /locus_tag="OQN02_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4078..4755)
                     /locus_tag="OQN02_000975"
     CDS             complement(4078..4755)
                     /locus_tag="OQN02_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1641865.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4743..5126)
                     /locus_tag="OQN02_000976"
     CDS             complement(4743..5126)
                     /locus_tag="OQN02_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1641866.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5142..5450)
                     /locus_tag="OQN02_000977"
     CDS             complement(5142..5450)
                     /locus_tag="OQN02_000977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641867.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5451..6029)
                     /locus_tag="OQN02_000978"
     CDS             complement(5451..6029)
                     /locus_tag="OQN02_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1641868.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6052..6483)
                     /locus_tag="OQN02_000979"
                     /pseudo
     CDS             complement(6052..6483)
                     /locus_tag="OQN02_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6855..7874
                     /gene="epd"
                     /locus_tag="OQN02_000980"
     CDS             6855..7874
                     /gene="epd"
                     /locus_tag="OQN02_000980"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1641869.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7924..9087
                     /gene="pgk"
                     /locus_tag="OQN02_000981"
     CDS             7924..9087
                     /gene="pgk"
                     /locus_tag="OQN02_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1641870.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9302..10381
                     /gene="fbaA"
                     /locus_tag="OQN02_000982"
     CDS             9302..10381
                     /gene="fbaA"
                     /locus_tag="OQN02_000982"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1641871.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10572..11432
                     /gene="mscS"
                     /locus_tag="OQN02_000983"
     CDS             10572..11432
                     /gene="mscS"
                     /locus_tag="OQN02_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1641872.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11571..12206
                     /gene="argO"
                     /locus_tag="OQN02_000984"
     CDS             11571..12206
                     /gene="argO"
                     /locus_tag="OQN02_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1641873.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12299..13039
                     /locus_tag="OQN02_000985"
     CDS             12299..13039
                     /locus_tag="OQN02_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1641874.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13206..14156
                     /locus_tag="OQN02_000986"
     CDS             13206..14156
                     /locus_tag="OQN02_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1641875.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14098..15576)
                     /gene="scpC"
                     /locus_tag="OQN02_000987"
     CDS             complement(14098..15576)
                     /gene="scpC"
                     /locus_tag="OQN02_000987"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS1641876.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15600..16376)
                     /gene="scpB"
                     /locus_tag="OQN02_000988"
     CDS             complement(15600..16376)
                     /gene="scpB"
                     /locus_tag="OQN02_000988"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1641877.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16387..17382)
                     /gene="meaB"
                     /locus_tag="OQN02_000989"
     CDS             complement(16387..17382)
                     /gene="meaB"
                     /locus_tag="OQN02_000989"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1641878.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17375..19519)
                     /gene="scpA"
                     /locus_tag="OQN02_000990"
     CDS             complement(17375..19519)
                     /gene="scpA"
                     /locus_tag="OQN02_000990"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS1641879.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19723..20616)
                     /gene="argP"
                     /locus_tag="OQN02_000991"
     CDS             complement(19723..20616)
                     /gene="argP"
                     /locus_tag="OQN02_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1641880.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20999..21658
                     /gene="rpiA"
                     /locus_tag="OQN02_000992"
     CDS             20999..21658
                     /gene="rpiA"
                     /locus_tag="OQN02_000992"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1641881.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21914..23146
                     /gene="serA"
                     /locus_tag="OQN02_000993"
     CDS             21914..23146
                     /gene="serA"
                     /locus_tag="OQN02_000993"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1641882.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23375..23434
                     /gene="ibsC"
                     /locus_tag="OQN02_000994"
     CDS             23375..23434
                     /gene="ibsC"
                     /locus_tag="OQN02_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1641883.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23767..23826
                     /locus_tag="OQN02_000995"
     CDS             23767..23826
                     /locus_tag="OQN02_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1641884.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23927..24529)
                     /locus_tag="OQN02_000996"
     CDS             complement(23927..24529)
                     /locus_tag="OQN02_000996"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1641885.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24550..24733)
                     /gene="ssrS"
                     /locus_tag="OQN02_000997"
     ncRNA           complement(24550..24733)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQN02_000997"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24775..25104)
                     /gene="zapA"
                     /locus_tag="OQN02_000998"
     CDS             complement(24775..25104)
                     /gene="zapA"
                     /locus_tag="OQN02_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1641886.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25272..25850
                     /gene="ygfB"
                     /locus_tag="OQN02_000999"
     CDS             25272..25850
                     /gene="ygfB"
                     /locus_tag="OQN02_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1641887.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25876..27201
                     /gene="pepP"
                     /locus_tag="OQN02_001000"
     CDS             25876..27201
                     /gene="pepP"
                     /locus_tag="OQN02_001000"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1641888.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27198..28376
                     /gene="ubiH"
                     /locus_tag="OQN02_001001"
                     /gene_synonym="visB"
     CDS             27198..28376
                     /gene="ubiH"
                     /locus_tag="OQN02_001001"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1641889.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28399..29601
                     /gene="ubiI"
                     /locus_tag="OQN02_001002"
                     /gene_synonym="visC"
     CDS             28399..29601
                     /gene="ubiI"
                     /locus_tag="OQN02_001002"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1641890.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29675..29758)
                     /locus_tag="OQN02_001003"
     CDS             complement(29675..29758)
                     /locus_tag="OQN02_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641891.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30049..31143
                     /gene="gcvT"
                     /locus_tag="OQN02_001004"
     CDS             30049..31143
                     /gene="gcvT"
                     /locus_tag="OQN02_001004"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1641892.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31167..31556
                     /gene="gcvH"
                     /locus_tag="OQN02_001005"
     CDS             31167..31556
                     /gene="gcvH"
                     /locus_tag="OQN02_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1641893.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31675..34548
                     /gene="gcvP"
                     /locus_tag="OQN02_001006"
     CDS             31675..34548
                     /gene="gcvP"
                     /locus_tag="OQN02_001006"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1641894.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34815..35557
                     /locus_tag="OQN02_001007"
                     /pseudo
     CDS             34815..35557
                     /locus_tag="OQN02_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="OQN02_001008"
     CDS             complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="OQN02_001008"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1641895.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37092..37402
                     /locus_tag="OQN02_001009"
                     /pseudo
     CDS             37092..37402
                     /locus_tag="OQN02_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37566..38225
                     /locus_tag="OQN02_001010"
     CDS             37566..38225
                     /locus_tag="OQN02_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1641896.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="OQN02_001011"
     CDS             complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="OQN02_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1641897.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39644..39910
                     /gene="sdhE"
                     /locus_tag="OQN02_001012"
     CDS             39644..39910
                     /gene="sdhE"
                     /locus_tag="OQN02_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1641898.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39891..40298
                     /locus_tag="OQN02_001013"
     CDS             39891..40298
                     /locus_tag="OQN02_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCS1641899.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="OQN02_001014"
     CDS             complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="OQN02_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1641900.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40971..41867
                     /gene="xerD"
                     /locus_tag="OQN02_001015"
     CDS             40971..41867
                     /gene="xerD"
                     /locus_tag="OQN02_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1641901.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41892..42602
                     /gene="dsbC"
                     /locus_tag="OQN02_001016"
     CDS             41892..42602
                     /gene="dsbC"
                     /locus_tag="OQN02_001016"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1641902.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42608..44341
                     /gene="recJ"
                     /locus_tag="OQN02_001017"
     CDS             42608..44341
                     /gene="recJ"
                     /locus_tag="OQN02_001017"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1641903.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44432..45530
                     /gene="prfB"
                     /locus_tag="OQN02_001018"
     CDS             join(44432..44506,44508..45530)
                     /gene="prfB"
                     /locus_tag="OQN02_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1641904.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45540..47057
                     /gene="lysS"
                     /locus_tag="OQN02_001019"
     CDS             45540..47057
                     /gene="lysS"
                     /locus_tag="OQN02_001019"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1641905.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47100..47648)
                     /gene="idi"
                     /locus_tag="OQN02_001020"
     CDS             complement(47100..47648)
                     /gene="idi"
                     /locus_tag="OQN02_001020"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1641906.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47703..47774
                     /locus_tag="OQN02_001021"
     CDS             47703..47774
                     /locus_tag="OQN02_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641907.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47771..47896)
                     /locus_tag="OQN02_001022"
     CDS             complement(47771..47896)
                     /locus_tag="OQN02_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641908.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47898..>48553)
                     /gene="uacT"
                     /locus_tag="OQN02_001023"
     CDS             complement(47898..>48553)
                     /gene="uacT"
                     /locus_tag="OQN02_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1641909.1"
                     /translation="IVFIESMGMFLALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFN
                     SFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAGL
                     VMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLH
                     SGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11647 a        12176 c        13111 g        11619 t
ORIGIN      
        1 gaatttgaac tgccagaaat taccgataat gaactgttag tgagtgtaat ttctgacagc
       61 gtctgtttat cgacctggaa agcggcgtta ctcggtagtg aacataaacg cgtacccgac
      121 gatttagaaa atcatccggt cattaccggg catgaatgtg ccggggttat tgtcgaagtg
      181 ggtaaaaatc tcactggcaa atataaaaaa ggccagcgtt ttgtattgca accggcgatg
      241 gggttaccaa gcggatattc agcgggctac agctacgaat attttggcgg caatgccact
      301 tatatgatta ttcccgaaat cgccattaat ttgggctgcg tattaccgta tcacggctct
      361 tattttgctg cggcgtcgct ggcagagcct atgtgctgca ttattggtgc ttatcatgcc
      421 aattatcaca ccacgcaata tgtttatgag catcgcatgg gcgtcaaacc tggcggcaat
      481 attgcactgc tggcgtgtgc gggtccgatg ggcattggcg ctatcgatta cgccattaac
      541 ggtggcatac aaccgtcacg ggtggtggtg gtcgatatcg acgacaaacg cctggcgcag
      601 gtgcagaagc tgctgccggt ggatctggcg gccagtaaag gcattgagct agtgtatgtg
      661 aataccaaag ggatgagcga tcctatccag atgctgcggg cgctgacagg agatgtcggg
      721 ttcgatgaca tttttgttta tgcggcggtg cctgctgtcg ttgagatggc tgacgaatta
      781 ctggcggaag atggctgtct gaacttcttt gccgggctga cggataaaaa cttcaaagtg
      841 ccgtttaatt tctacaacgt ccattacaac agcacgcacg tagtcggtac atccggcggt
      901 tcaacggacg acatgaaaga ggcgattgcc cttagcgcca caggacagtt acagccgtcg
      961 tttatggtga cccatattgg tggcctggat gcggtgccag aaaccgtgct caatctgccg
     1021 gatatccctg gcggtaaaaa actcatttat aacggcgtga ccatgccgct cactgccatt
     1081 gccgattttg ccgaaaaagg caaaaccgat ccgctgttta aagagttggc gcggctggtt
     1141 gaggaaacgc acggcatctg gaatgaacag gccgagaaat atctgctggc acaatttggc
     1201 gttgatatcg gggaggccgc gcaatgatgt ccctggcgtg gccattattt cgcgttacgg
     1261 aacaggcagc gttggctgcc tggccgcaca ccggatgtgg cgacaaaaat aaaattgatg
     1321 gcctggcggt caccgcgatg cgccaggcat taaacgacgt cgcttttcgt ggacgagtgg
     1381 ttatcggcga aggagaaatt gaccatgcgc cgatgctgtg gattggcgaa gaggtgggca
     1441 aaggcgatgg gccagaagtc gatatcgcgg ttgatcccat tgaaggcacg cggatggtgg
     1501 cgatggggca gagcaatgcg ctggcggtca tggctttcgc tcctcgagat agcctgttac
     1561 atgcgcccga tatgtatatg aaaaagctgg ttgttaatcg actggctgcc ggggcgattg
     1621 atctgtcttt gcctctggcg gacaacttgc gtaacgtggc gcgggcgtta ggtaaaccgc
     1681 tggacaagct gcgcatggtt acgctggata aaccgcgtct gagtgccgcg attgaagaag
     1741 caacacagct gggcgtgaag gtttttgccc tgccggatgg cgatgtcgcc gccagcgtgc
     1801 tgacctgctg gcaggataat ccttacgatg tgatgtacac catcggcggt gcgccagaag
     1861 gcgtgatttc tgcctgtgcc gttaaagcgt taggcgggga tatgcaggcc gagctgattg
     1921 acttttgtca ggctaaaggg gattacacgg aaaatcggca gattgcagag caggagcgca
     1981 agcgttgtaa ggcaatgggc gttgacgtta accgtgtgta ctcgctcgat gaactggtga
     2041 gggggaacga tatcctcttt agcgctacgg gcgtgacggg cggcgagttg gtgaacggta
     2101 tccaacagac ggcgaatggg gtgcggacgc agacattact gatcggcggc gcggaccaaa
     2161 cgtgtaatat aatagactcc ctgcattgat ggtgatctat cggaagacca atggcgacgc
     2221 tgacagaaga tgatgtgctt gagcaactgg atgcacagga caatttattt tcatttatga
     2281 aaactgcgca ttctattttg ctccagggga tacgccagtt tctgccgtcg ctgtttgtcg
     2341 ataacgatga agagatcgtc gaatatgcag tgaagccgtt actcgcccaa agcggcccgc
     2401 ttgacgatat tgatgttgcg ctgcgtttga tttatgcgct ggggaaaatg gataaatggc
     2461 tgtatgccga tatcacgcac ttttcccagt actggcatta cctgaacgaa caggatgaaa
     2521 cgcccggatt tgccgatgat ataacctggg attttatcag caatgtcaac agtataactc
     2581 gcaatgcgac gctctacgat gcgttaaaag cgatgaagtt cgccgatttc gccgtctggt
     2641 cagaggcgcg ttttagcgga atggtcaaaa cggcgctgac gctggcagta acgaccacct
     2701 taaaggaatt aacgccgtga aaattgaatt aacggtgaat gggctgaacg tgcaagcgca
     2761 gtaccatgat gaagagatag aacgcgtgca taaactgttg ctgcgtatgc tggcggcctt
     2821 acagacggta aatccgcagc ggcgtacggt ggtttttctt tgcgctccgc cggggacggg
     2881 taaatctacc ctaaccacct tctgggaata tctcgcgcaa caagacccgg agctgcccgc
     2941 tattcaggcg ctcccgatgg atggttttca ccattacaat agctggctgg acgcgcatca
     3001 attgcgcccc ttcaaaggcg caccagagac atttgacgtt gcgaaactgg cggaaaatct
     3061 gcgccaggtg gtggaagggg attgtacgtg gccgcagtac gatcgacaaa agcatgatcc
     3121 tgttgaagat gcgttgcacg ttaccgcacc actcgtcatc gtcgaaggaa actggttgtt
     3181 gctggacgac gagaagtggt gccagcttgc gcaattttgt gatttctcga tctttatcaa
     3241 cgcgccagct gctgcgttgc gggagcgtct ggtggggcgc aagctggcgg gaggggtatc
     3301 gctggcagat gcagaagcct tttatgaccg taccgacggg ccgaatgttc gccgggtgct
     3361 ggaagagagt ctgcctgcaa atttgacctt aatgatgacc gctacgggtg aatatcgtct
     3421 ggtggattaa cgttcctgat tacaccatct tccagccgca ccacacgaga gaaatgacgc
     3481 cgggtaaatg cggcgtcatg actgattgct accacgctcg tgcctcgctg gcggcacttc
     3541 tccagccagc tttcaaacac gctcaaccaa ttttcgtcaa aatcccgact cggctcgtcc
     3601 agcagtaata gtggcggaga gagtgcttcc aggcaggcga cggcgaccat ccgtcgctgc
     3661 gcactatgca gatccaacgg gtgcgagttg gcagtgtctg ctaactgaca acattgcagg
     3721 gcagcatggg tacgttgagt gatttcatcc gccgggcatt tttgcagttt caaaccaaat
     3781 gcgatctcgt cagcgacggt gctgtgaaaa agttggtttt ccgcttcctg aaacagaaca
     3841 gtacgccaac ctttgccgcg cgctggcggt ttttcaggtt tttcatcgcc tgttgttgca
     3901 gcataaccgt accggctgtg ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt
     3961 tccctgcgcc attgtcaccg gtcagcgcca accattcacc ttgtttgagt tgcagagaaa
     4021 tgtcgcaaag acaatccgtt gccgcgccag gccagcgata agatatctta tttaacgtta
     4081 acatagtggc tggagcgctc cgttttgcaa ttgccacgct tgctggcagt agttcaggaa
     4141 aggtgtgcga tgacgttcaa aaagaataat cagcgaacca cgttcaaacg cccagtgttg
     4201 taagcgctgc aacagcattt cgctggcctg cggcgtcagg cggctaaagg cttcatcaag
     4261 aattaagagc tttggctgca tcgcgatggc gcaggcgatg atcacgcgct gtgtctctcc
     4321 accggagagt gttgccgggt gacgatggcg caacggctgg cactcggtca gagtcagggc
     4381 ggcatcaatc cgcgccataa tctctgcttc agcaagacac agattttctg gcccaaaggc
     4441 aacttcttct tccacgctaa aggtacagcc ggagagctgc aaatagggcg attgctggac
     4501 gagctggatg gtggcggatt gttcattaag cgggagatgc ccaatgggcg taccgaggag
     4561 cgtccccgtg cctgttatct ctcttggcag aaagtccgga taccagcccg ccatcagctg
     4621 tgccagcgta cttttgccac tgccgttgtc accaaagatg gcgaccatcc ccggtttgac
     4681 atagtgaaag tcataacaga acggaggatg cgttgagtgt gtcgggcaat aacggaactg
     4741 ttctaacgta accatatcca cgctccgaat tctgtcagca tcagcaagat catggtgtaa
     4801 cgcgctacgc gttgtagggt gctatcggct ggtgcccaca acgttgttcg tcggttgtgg
     4861 atccgaaatg cccgcatatc cagcgccgca ccacggacag ccagatcgtt aagcgcattg
     4921 tgtgttagcg gaataattaa cgcgggcata gcacgtaaac gctgatacca gccctcatcc
     4981 agcggcacgc cgcgggcacg ctgcgcttca tgaataatcg ctaattgccg ttttaactgc
     5041 tcaacgacca ataacggccc ggcaaacaga taagcgacgc ctggcggcag gcgagaagca
     5101 aacagagcgc gaataaaaca ttgcacgggg acgtactgca ttcacaactg tgaggttgaa
     5161 acaatcgcca gaatcagcaa ccagagcgta atagcatgcg accaccgttc tggcgagcgt
     5221 ggcgtgccgc ttagccattc tggtagccag ccaccgtgta ccagccacag cccggctccc
     5281 agcgagaaca tcagccagac gacatatttt gcccgccgcc gcgtggcctt tagcgcaata
     5341 aggcagaaaa aggtcgccgc gctatagatc ggcaaaatgg tctgcgcggg caggataagc
     5401 gtggtgcagg ccgccaatgc ccacagtgtt aatgaggtaa atggatgcat ttagcgcacg
     5461 gcagccattg ccggaaaatg gcgcgtggtg cgaatcggca gctggcgcag taaaagccag
     5521 acaatcaccg ctgtaagaat tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc
     5581 gactccacca ggttttgccc catcgagtgc atccaggcaa cgaacaaatc ggctccgcta
     5641 ccggtgacac cgccgaacag cgcagtacgc agcggtacag cgacgaccgt taccgcaagc
     5701 gtaataatca cgccgctgac aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc
     5761 agccaaccgg agaccaggcc gatgaccatc gcgacgggtg caaatgcggc ggcgatcggg
     5821 tcggtcagca atccccagag taaattggtc agtaagccgg tcagcattcc gatcaccgga
     5881 ccaagcagta ccgcgctaat aagtgtgccg atggagtcga gaaatatagg taatttgacc
     5941 atgctggcca gttgcccgcc gatcatattg atggcgatag atataacgat caacaccagt
     6001 gcctgactgg aaaagtggct acgcgccata agaaaacctc tttgaaagta gttatttaac
     6061 gggggaaata tgcaactcgt cgtaaccaga aagcgcgcaa ggttggcggc taaacgccag
     6121 ttcctcaagg ctaccgatca ccagttcaag cgtggtccgc acggtacagc cgggcatcat
     6181 atgaccgcca agcatcgtgc cgtgtggatc ggaaacgcag agatgaagat gctcaccact
     6241 ctgttccagc gtgccgttta gtgagatcac ttcgaatgta ccgttcagga gcgtggtgcc
     6301 ttcttgcccg gcatagcgca aggcgacgtc agtcaggcta ccggtacagc ctgcaatcta
     6361 ggcggcatga agttgatgtt gctgtgcaaa agcgtgtagc tgggaaaaaa cttcctgccc
     6421 ggggaggagg cgcagtgcgt aaaagcgtgc ggaggaagaa tatgagtagg gcaatgtcat
     6481 cattggagta ctccgaaact cggacgtatt gcggataata tcgacgctgc atcagtcata
     6541 ttctgatata agtcagggtt tgtgacggca caacgaaaag attttgctac gcttattcgt
     6601 gattctgcaa agtggaataa attccacagg ggcggggctg aaaacgtgac gtaagtcaga
     6661 taattgcttt ctttgactgg acaatcattc cttttattcc acgtttcgct tatcctagct
     6721 gaagcgtttc agtcgattaa atgttcgaca attaaccaat cagtcgcagt ttgcgacagg
     6781 taaggtttcc ccggacgatt tgctggatta ctctgtctgg caaatttgct cagggaaaac
     6841 cttgcaggag atctatgacc gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta
     6901 atgtggttcg tgctttgtat gaatccggac gccgggcgga aattaccgtg gtggcaatca
     6961 acgaactggc ggatgctgcg ggcatggcgc atttgttgaa atatgacacc agccatggcc
     7021 gttttgcatg ggaagtacga caggaacgcg atcaactttt tgttggtgat gacgccatcc
     7081 gcgtattgca tgaacgttca ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag
     7141 tcctcgactg caccggcgta tatggctccc gcgagcatgg cgaagcgcat attgccgccg
     7201 gggccaaaaa agtgctcttt tcacatcctg gcagtaacga tctcgacgcg accgttgttt
     7261 acggcgtcaa tcaggatcaa cttcgtgcgg aacaccgcat cgtttctaac gcttcctgta
     7321 ccacgaattg cataattccc gtcatcaaat tgttagatga tgcgtacggt attgagtccg
     7381 gcactgtgac cacaattcac tccgccatgc acgatcaaca ggttattgat gcataccatc
     7441 ctgacctgcg tcgcacccgg gcagccagcc agtcgatcat tccggtcgat actaaactgg
     7501 ccgctggtat cacacgattt tttccgcaat ttaacgatcg ctttgaagcg attgcggtac
     7561 gtgtgccaac cataaatgtg acggcaatcg atttaagcgt gacggtgaag aaacctgtaa
     7621 aagccaatga agtcaacctg ttgctgcaaa aagcagcaca aggtgcattt catggtatag
     7681 ttgactatac ggaattgccg ttggtctctg tagattttaa ccacgatccg cacagtgcca
     7741 ttgtcgatgg cacccaaacc cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt
     7801 gcgataacga atggggcttt gctaaccgaa tgctcgacac gacgttagct atggctactg
     7861 ttgctttcag gtaagacgca agcagcgtct gcaaaacttt tagaatcaac gagaggattc
     7921 accatgtctg taattaagat gaccgatctg gatcttgctg ggaaacgtgt atttatccgt
     7981 gcggatctga acgtaccagt aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct
     8041 tctctgccga ccatcgaact ggccctgaaa caaggcgcaa aagtgatggt aacttcccac
     8101 ctgggtcgtc ctaccgaagg cgagtacaac gaagaattct ctctgctgcc ggttgttaac
     8161 tacctgaaag acaaactgtc taacccggtt cgtctggtta aagattacct cgacggcgtt
     8221 gacgttgctg aaggtgaact ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag
     8281 aaagacgacg aaaccctgtc caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac
     8341 gcattcggta ctgctcaccg cgcgcaggct tctactcacg gtatcggtaa attcgctgac
     8401 gttgcgtgcg caggcccgct gctggcagct gaactggacg cgctgggtaa agcactgaaa
     8461 gaacctgctc gcccgatggt ggctatcgtt ggtggttcta aagtatctac caaactgacc
     8521 gttctggact ccctgtctaa aatcgctgac cagctgattg ttggtggtgg tatcgctaac
     8581 acctttatcg cggcacaagg ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt
     8641 gacgaagcta aacgtctgct gaccacctgc aacatcccgg ttccgtctga tgttcgcgta
     8701 gcaaccgagt tctctgaaac tgcaccggct accctgaaat ctgttaacga tgtgaaagct
     8761 gacgagcaga tcctggatat cggtgatgct tccgctcagg aactggctga aatcctgaag
     8821 aatgcgaaaa ccattctgtg gaacggtccg gttggcgtgt tcgaattccc gaacttccgc
     8881 aaaggtactg aaatcgtggc taacgctatc gcagacagcg aagcgttctc catcgctggc
     8941 ggcggcgaca ctctggcagc aatcgacctg ttcggcattg ctgacaaaat ctcctacatc
     9001 tccactggcg gcggcgcatt cctcgaattc gtggaaggta aagtactgcc tgcagtagcg
     9061 atgctcgaag agcgcgctaa gaagtaaaaa atcacagggc agggaaacct gcacttgttt
     9121 cagcgcgctt ttagagcacg cattttttca tactctaaat aattcgagtt gcaggaaggc
     9181 gacaagagag tgaatcccca ggagcttaca taagtaagtg actgggggga gcgaacgcag
     9241 acgcagcaca tgcaacttga agtattatga gtataaggcc cgacgataca ggacaagaga
     9301 catgtctaag atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa
     9361 agttttccag gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac
     9421 tgactccatc aacgccgtac tggaaactgc tgctaaagtt aaagcgccgg ttatcgttca
     9481 gttctccaac ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca
     9541 gggtgctgct atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca
     9601 ttatggtgtt ccggttatcc tgcacactga ccactgcgcg aagaaactgc tgccgtggat
     9661 cgacggtctg ctggacgcgg gtgaaaaaca cttcgcagct accggtaagc cgctgttctc
     9721 ttctcacatg atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa
     9781 atacctggag cgtatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg
     9841 tggtgaagaa gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc
     9901 ggaagacgtt gattacgcat acaccgagct gagcaaaatc agcccgcgtt tcaccatcgc
     9961 tgcgtccttc ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac
    10021 catcctgcgt gattctcagg aatatgtttc taagaaacac aacctgccgc acaacagcct
    10081 gaacttcgta ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag
    10141 ctacggcgta gtgaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt
    10201 tctgaactac tacaaagcga acgaagctta tctgcagggt cagctgggta acccgaaagg
    10261 cgaagatcag ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac
    10321 ttcgatgatc gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta
    10381 agtcattcct ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca
    10441 aggtgattca gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc
    10501 ttgtcagact gcccgtcata aggcggcgga gtgtatttct ccattttgag tcagttgaaa
    10561 aggaatattg aatggaagat ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc
    10621 tggtagctaa ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga
    10681 tcatcatcgt tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga
    10741 tctcccgtaa aatcgatgcc actgttgctg attttctttc tgcattagtc cgttacggta
    10801 ttatcgcctt tacgctaatc gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca
    10861 ttgctgtact cggtgccgca ggcttagctg ttggtctggc tttgcagggg tcactttcta
    10921 acctggccgc tggcgtgtta cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg
    10981 acctcggcgg cgtagccggt actgtgctga gtgtgcagat tttctccacc accatgcgta
    11041 ctgcagacgg taaaattatc gttattccga acggtaaaat tattgccgga aatattatta
    11101 acttctcccg cgagccagtt cgccgtaacg aatttattat tggcgtggcg tatgattccg
    11161 atatcgatca ggttaagcag atcctgacca atattatcca gtctgaagat cgcattttga
    11221 aagatcgcga aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg
    11281 tccgcgtctg gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta
    11341 ttaaacgtga atttgatgcc gccggtatca gcttcccgta cccgcaaatg gatgtgaact
    11401 ttaagcgggt gaaagaagac aaagctgcgt aatcaacgct gaggccagat aatactctct
    11461 ggcctctctc ttattagtta ttctgattgc caattaatat tatcaatttc cgctaataac
    11521 aatcccgcga tatagtctct gcatcagata cttaattcgg aatatccaac gtgttttctt
    11581 attactttca aggtcttgct cttggggcag ctatgattct gccgctcggc ccacaaaatg
    11641 cttttgtgat gaatcagggc attcgtcgtc agtaccacat tatgattgcc ttactttgcg
    11701 ctatcagcga tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc
    11761 agtcgccgtg gttgctagcg ctggtcacct ggggcggcgt agtcttcttg ctctggtatg
    11821 gttttggcgc ttttaaaaca gcgatgagta gtaatattga gttagctagt gccgaagtcc
    11881 tgaagcaagg cagatggaaa attatcgcca ccatgttggc agtgacctgg ctgaatccgc
    11941 atgtttacct ggatactttt gttgtactgg gcagccttgg cgggcagctt gacgtcgaac
    12001 caaaacgttg gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg
    12061 ctattctcgc agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca
    12121 atctggtagt gggatgtgtc atgtggttta tagccttgca gctggcgaga gacggtattg
    12181 ctcatgcaca agccttgttc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct
    12241 aagcttgcct ccagaggtcc tgaattttgg gcaagtcaat taacacggag agactaacgt
    12301 gaagttcaaa gttatcgccc tggcggcatt aatgggtatt agcgggatgg cagcgcaggc
    12361 taacgaattg ccggatggac cgcatattgt cacctccggt acggcaagtg tggatgcggt
    12421 gccagacatt gccactcttg cgattgaagt taacgtggcc gcgaaggatg ccgctacagc
    12481 caagaaacag gcagatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat
    12541 cgcgaaaaaa gatatcagct cagcgaattt acgcacccag ccagattatg actatcagga
    12601 tggtaaaagt atcctgaaag gctaccgagc tgtgagaacg gtggaagtca cgctccgtca
    12661 gttagacaaa ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg
    12721 ttctgtgtcg ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc
    12781 gattgataac gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg
    12841 gccggtatat agcgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat
    12901 gatgaaagcc gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg ccgctattca
    12961 gtttgatgat caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa
    13021 aacacctgca gcacaataat acttgtagcc cgacgtattc gtcgggctaa tgcctttacc
    13081 cgatattgcc tcctttttct gtaattttct gtaaaaccat gacgtaatca cctatctttg
    13141 ctcgcggttt catcaaggat aggctgactg aaacggttaa gcggacaagg atgcggggag
    13201 gggggatgga catttttatt tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca
    13261 ataatattgc ccgggcggca gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca
    13321 ttgccgcgct ggaagaacaa attggctata cgctatttac ccgcaaagat aataatatca
    13381 gcctcaataa agccggtcag gaactgtatc aaaaactgtt cccggtttat caacgacttt
    13441 ctgcgattga taatgaaatc cataactcag ggcgtcgttc acgagaaatt gtgattggca
    13501 tagataatac ttatccgaca attattttcg atcagttgat tagtctcgga gataagtatg
    13561 aaggcgtgac tgcccagcca gttgagttca gcgaaaacgg cgttatcgat aatctttttg
    13621 accgtcagtt agattttatt atctccccac agcatgtatc tgcacgagtt caggaactgg
    13681 aaaacctgac tatcagtgaa ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt
    13741 atgaagagcg tcaagagcaa gagctgcttc aggagctgcc ctggctacaa atgcgctttc
    13801 aaaaccgggc taacttcgaa gcaatgattg atgcgaatat gcgaccttgt gggatcaatc
    13861 ccaccattat ttatcgtccc tacagtttta tggccaaaat cagcgccgtg gagcgcggac
    13921 attttctgac ggtgatcccg cattttgcct ggcgactggt gaatccggca acgctgaaat
    13981 attttgatgc gccgcacagg ccgatgtata tgcaggaata tctttattca atcagaaatc
    14041 atcgttatac cgccacgatc ttcagcatat tgctgaagat cgtgacggga caaaccatta
    14101 acccagcatc gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc
    14161 gtggtgaata tgtccgccgg gcgcattttc cagatagcga tgcagataat cccgatacat
    14221 aggatgtgca caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag
    14281 atcggcgatc ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt gatcaacatg
    14341 gctgcacatt ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gagccataaa
    14401 gatcgacaga tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc
    14461 gaccccggct acgtgcgttg aatttgcatg cccgtaaata tcaaactcca ggccgacgtt
    14521 cagagcgatg acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg
    14581 caacacaatg cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc
    14641 cgagattgtc aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac
    14701 caccgattcc tgtagcactt ccgaatacat catgaacgga ggaatttccg ggttttcccc
    14761 cagacgtgcc attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc
    14821 cggcggaata cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat
    14881 ctgctggcac atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc
    14941 cacgacggcg acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat
    15001 tgcatggaag atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatctgc
    15061 cagttctgca acgcgcggat cgtgatagtg attaagttca atgatcactt tcttcgctcg
    15121 cagcagccag gtcggcgcat taccgatccc gctggttaac cagactcgac catccggtgc
    15181 cagtgccgat gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat
    15241 ttgcgccact tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt
    15301 acgtaaactg gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag
    15361 tacatcgtca gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt gatacggctt
    15421 tttggcctca tgctgttcgt tagctctgcg ggcaatcgcg gcgggtaggg ctttcggcga
    15481 acccgctggg gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc
    15541 ttcatcggcg gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat
    15601 taatgaccaa cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa
    15661 tcttcgctgt cataaaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc
    15721 atggtgtgtg cttcgcccag tacacgcagc tcttctttga taacggcaat ggctaacggc
    15781 gctttctcag agatgtggtg cgccatttgt aaggtgaaat cttccacttc cacaacatgg
    15841 ttgaggatgc cgacagccag cgcgcgctgg gcggtgattg gcgaagcggt aaaaatcagc
    15901 tctttgacaa tgtggaagcc cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac
    15961 gggacgccga ggtttacagg cgtcattgag aaggttgagg tactggcggc gatgatcaga
    16021 tcggaactca tgatcatttc aaatgcgcca ccccaaacac taccttccac catcgaaatg
    16081 atcggtttcg ggaatttttg gatcatgcgg gtgatttgac gcaatggatc atcataggag
    16141 agcggatcgc gaccgccaga cggcagttcg tgaatatcgt gacctgcgga gaagactttg
    16201 gatccacttg gcgcccgcaa aatgatacag cgaatttccg gccggttgag atcgcttaac
    16261 gcctgcataa gatcatcaat aaagacttta cttaaggcat taagttttcg tccatagtta
    16321 aactcaatga ccgccacttt gttgatagtg gcaacgttca catactgata agacataaaa
    16381 attcctttaa tcaaaatatt gcgtctggat aaattcactg agctgccgca ggccggtgcg
    16441 cggtgagagc gtattgtttt tgaccgctaa aagcgtctgg tggtaatagc gatcgaaatc
    16501 ttcattcgcg aacaggtgat tcagtacttc ttcttcggtc tgcttacgca gccattccac
    16561 cgattgttgt tgccgcactt gttgtaaacg accactggca gttagcgcgg ttttgaagtc
    16621 gatgatggcg tgccagatct catcgattcc acgtttttcc agtgcgctac aagtcagacc
    16681 ccgtggctgc cattcgtcgt atttacgtcg cagaatatgc agggcactct cgtacatatg
    16741 ccgggcaatg gcgacattgg tatggttatc gccatcgtct ttgttgataa cgagcagatc
    16801 agccacttcc atcagccctt ttttaatgcc ctgcagatca tcgccgccac cggcaatttg
    16861 caacgagata aaacagtcca ccatgcgggc gacttccgtt tccgactgcg cgacgccaac
    16921 cgtttcgaca atcactacgt cataacccgc cgcttcgcat aacagcatta attcccgtgc
    16981 tcgctgactg gcaccgccca gatgaccgga ggagggtacc gggcgaataa acgccgcctc
    17041 ggcacgcgcc aggtcattca tgcgggtttt atccccgaga atgctaccgc cagtgaccgg
    17101 gctgctggga tcgaccgcaa taacagcaac ctttaatccc tcacgaatca acaacatgcc
    17161 aaaggcttca agaaaggtac ttttccccgc gccgggtgtg ccggtaacgc ccagtcgcag
    17221 ggcgttacca cagaacggca taatagcgtc aagcagctgt gtacttagcg cctgatgacg
    17281 tgggtgacgg ctttccacca gcgtcatggc ttgggcgagc gtggcacgct caccctgacg
    17341 taagcggcga atactttctg ccagcgtggc ttcattaatc atgatgctgg cttatcagat
    17401 tcagtacgtc gcgcacactg tcgagcatag gtgtacctgg accataaatc gccgccacgc
    17461 cgcgctcttg caggaaggcg taatcctgcg gcggaatgac gccacccgcg accacgcaga
    17521 tatcttcgcg tccccatttt ttcagcgctt cgaccagttc cgggatcagc gttttatgac
    17581 cggcagccag tgaggatgcg cccactacgt gaacgtcgtt ttctacggcc aggcgggcga
    17641 tctcttcagg tgtagagaac atcgggctta aatctacgtc gaaaccgaga tcggaatagg
    17701 cgctggcgat cactttcgcg ccgcgatcgt gtccatcctg gcccatctta gcgatcagaa
    17761 tgcgcgggcg acgaccattg tcggcaagga actgctccgt ttgcgcaaca atggcatcga
    17821 actcggaggc cgatttctca gactgatgat agctttgcgc aatcacgccg gtaacacact
    17881 ggcttggcac cagataacgg tcgaaagcga cttccagcgc atcggaaatt tcacccaggg
    17941 tggcgcgaac gcgagcggca ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg
    18001 cgtgagtcag ggcgttcaac gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc
    18061 gttccagcga agcaatttgc tcgttacgca ccatcacgtt gtcgatctca agtacatcgg
    18121 tttcgtcttc gtgatccagt ttgtacttgt tgacaccaac gatgacacgc ttgccctggt
    18181 cgatcagcga ctgttcgcgc gctgaggcct cttcgatcat tcgttttggc agacctgctt
    18241 cgatcgcttt cgccatgcca ccggcttcgt cgatctgttg gataatagct ctggcttgtt
    18301 tgacgatttg atcggtcagc gactcaatgt aataggatcc ggccagtgga tcgacggtgt
    18361 ggcagagttc tgattcttcc tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga
    18421 aatcggtagg caaaccaagc gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc
    18481 ccagcgtcgc agccagtgct tcaatggtgg tgcggataac gttgttatac ggatcctgtt
    18541 cagtcaggct ccagcctgag gtctggcagt gggtacgcag cgccagtgat ttcggatcct
    18601 gtgcgccaaa tccactgacc gcttcgctcc ataaataacg tgccgcacgc aacatggcga
    18661 cgttcataaa cagatccatg ccgataccga agaataacga caggcgagga gcgaagtcat
    18721 caattttcag tccggcagag attgctgctt tgatgtactc aatcccatcg gcgatcgtaa
    18781 atgctacctg ctgcacgcag ttggcacccg cttcccccat gtggtaaccg ctgatactga
    18841 tggtattaaa tcgcggcatg ttgccggaac accaggcgat gatgtcggcg ataatgcgca
    18901 ttgacggttt tggtgggtaa atataggtgt tgcggcagag gtactctttg agaatatcgt
    18961 tttgaatggt gccggtcagt ttatcaggtg taacaccttg ctcttctgcg gcgacgatat
    19021 aaaacgccag tactggtagc actgcgccat tcatggtcat cgaaaccgac attttatcca
    19081 gcgggatctg gtcgaacagg actttcatat cttccacggt gtcgatagcg acgcccgctt
    19141 tgccgacgtc acccgccacg cgtgggttat cggagtcgta gccacggtgg gtggcaaggt
    19201 caaacgcaac ggaaagacct ttttgcccgg cggccaggtt acggcgataa aaagcgttgg
    19261 actcttttgc tgttgaaaaa ccagcatact gacggatggt ccacggttgg gcggtataca
    19321 tagtggcacg cgggccacga acgtagggcg gcaaaccagg aagggtacct gtcacctcca
    19381 gattatcgag atcggcttcg gtatacagcg gcttgatggc gatcccttcc gcggtttgct
    19441 gaaccagcga gtcgacagtt ttctcccgac ggctcaattc cttgttggca agctgttgcc
    19501 actcctgctc gttagacatt atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg
    19561 cttacggtga gctttttacg tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt
    19621 tttttgccgc ctgagatttg tctattttgt gatgtgattc aaggaataca aaaagaagtg
    19681 aggacaacgc ctgatatgtg ctatcaggca ttatttgatg gattaatcct gacgaaggac
    19741 tttgtgacca taatcgagta acgcatcagt gactttacgc atcatgcggc tttcaggagc
    19801 aaagcggtgc cagtagagca tccgtcgttg aaatagccca ggcgttaagt caatcagttc
    19861 accgctggcc agctctttct cgatttgcag gtgcgggatc atacagcagg tggtgccctg
    19921 gcgagcaagt tgtacgaacg cttctgaaga attaacgata tggcagggta cgctgccagg
    19981 aggaagatcg aagttttgct gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc
    20041 gaccactggc gctttcaata atgccgaacg cgttacgccg ttaggaaaat atttttcggc
    20101 aaacggtttt gagctgacga acagatagtc gagcgcaccg agtttatcga caagacaact
    20161 cggcagcgcc tgatgttgaa tactcaccgc gccgaccact tcgccgcggc gcagacgttc
    20221 ctgagtacgg gtttcatctt ctacctgcaa gttgaggcgg ataggcgaat cagccaacac
    20281 aggagccagt gccggaagca accacgtcgc cagactgtcg gcgttgaccg ccagtgaaag
    20341 cagcagcgga gtcgaaccgg tttgttcatc gcccagccac tcttcttcca gcaactccac
    20401 ctggcgcagc agtgccagca gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg
    20461 caccaacagc ggctgcccga acatattttc cagttgctta atgcgctgtg agacggctga
    20521 ttgtgtaatg cacagctttt gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc
    20581 cagtgcctgt aatgttctgt agtccgggcg tttcattgct gttgtctgct cctggtaaat
    20641 gctttttcct gcactatgac acaattttgt gtcaggttgc atataccgaa gccgtcggcg
    20701 ggtaacattt gtatcaatgg ttaaacgcgc agcttaaaag atcagggatt tgcgctattg
    20761 catttcgctt agcgacttcg cgccctggtt atgttgaatg gcgtggcgtt attgcctctg
    20821 ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg gcgaaagggg
    20881 atgcccgcca ttgcgcgata ttttatggat gagttaacca cccagcttgc caacggggtc
    20941 tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg aaacgatcat
    21001 gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg ttcagcccgg
    21061 taccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg cgctcggcac
    21121 gatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg aaaaactgaa
    21181 aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca tctacgttga
    21241 tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg cggcgctgac
    21301 ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgccg acgcctccaa
    21361 gcaggttgat attctgggta aattcccgct gccagtagaa gttatcccga tggcacgtag
    21421 tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc agggcgtggt
    21481 gaccgataat ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg acccgatagc
    21541 gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt ttgctaaccg
    21601 tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc
    21661 tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca
    21721 cgcttttacc aggcaattgt cgattgctct aaataaatcc tctaaaccag catattcatc
    21781 caagaattac ctttgcgtga tatttcctca acatcgcgac gcaaacgttc atattgccgc
    21841 aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca tttcaaaaga
    21901 caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt ttctgctggt
    21961 agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca ccaacatcga
    22021 atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg atgcccactt
    22081 catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg cagaaaaact
    22141 ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg cggcggcaaa
    22201 gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg cggagctggt
    22261 gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg
    22321 tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat
    22381 catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc tgggaatgta
    22441 tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc aggtacagca
    22501 tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag agaatccgtc
    22561 caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aagcccggct cgctgctgat
    22621 taatgcctcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa
    22681 acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca atagcgatcc
    22741 atttacctct ccactgtgtg aattcgacaa cgtccttctg acgccacaca ttggcggttc
    22801 gactcaggaa gcgcaggaga atatcggctt ggaagttgcg ggtaaattga tcaagtattc
    22861 tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc cactgcatgg
    22921 tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa
    22981 aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt ccgcccagat
    23041 gggttatgta gttattgata ttgaagctga cgaagacgtt gccgaaaaag cgctgcaggc
    23101 aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc ccttctctga
    23161 aaattaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac accgttgccg
    23221 cgttgcgcat tctactttgc gagtctgctc cagaaaataa atcaaagtca gatcaatgcg
    23281 ttgtgtttat ttaatgggga tttacgatgg cgggacagca tggggctgta acggtaaagc
    23341 cctcaccgaa gcgagggctt aaaggagaag ggttatgatg cgacttgtca tcatactgat
    23401 tgtactgtta ctgataagtt tcggagctta ttaacagtca gtctcaaggg aggagtcatc
    23461 ctcccttacc cttactcact aaattaggtc aaagaatcaa cgatgtcaat cagggcgatg
    23521 cggggtgcat cgccctgaac cgaacttttt gatagccact tcatcgccca cgtttactgc
    23581 cgtaatagat cctttttccc aaaaattatc tctgttttgc atggggtttt tcgagcacgc
    23641 tcgaagaaaa gatttaaagg tagatcaatg cgttgtggtt atttaaagag agcttacgat
    23701 agctgggcag catggggctg taacggtaaa gccctcaccg aagcgagggc ttgaaggaga
    23761 agggttatga tgcgacttgc catcatactg attgtactgt tactcataag tttcccagct
    23821 tattaacagt cagtcacagg ggaggagcaa tcctccctta cccttactca ctaaattagg
    23881 ttaaagaatc aacgatgtca atcagggcga tgcaggtgta tcgcccttac cactcccaga
    23941 ctttcgacgg tgtaaccacc gcaggaagag ggatatccca ctcttcaacg gggagttttt
    24001 ccaccaactg acaatcatgc gcataaccca ccggttgcgt tttatagtgc tgccagtttt
    24061 gtaaggtccg atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac tcatcaaagg
    24121 cgaccagcgg tgtgatcagc acgtctaatc gggaaagggg tagcacgtca cgcacatcca
    24181 gttttggctc atggatcttc aacctgttca tcaccagttc gctttgcgga tggtaattca
    24241 ggaacagcaa attaccggca ctaaagggat gcaaaactgg aagatatacg cgcttaccgg
    24301 cgcgccagag ttgttctatt agcggctggg tgtcgagttc accatcaaaa gagaggaata
    24361 cagcgaccgt atgcgccatc accaccgggg gataagtcat catccgggta gcggcttgtt
    24421 gacccatttc cagctgttgt tccggcgtta acgcacgacg acgttgccga atcattttgc
    24481 ggatttcttg tcgggataat gtcagtggga gctctggtag ttgcgtcatg gctggtgcca
    24541 gataagaagg gaatctccga gatgccgccg caggctgtaa cccttgaacc cttggttcaa
    24601 ggtgaatgtg tcgtcgcagt tttaaggctt ctcggacgaa ccgagcatgc tcaccaaccg
    24661 cggagcgcca cattcttgtg gtatgaaata tcggctcagg ggactggccc gcttgcgaac
    24721 atctcagaga aattttgtct tcacggttac tctaccacag taaaccgaaa agtgtcattc
    24781 aaagttttgg ttagtttttt cggtgatgcg accttgttca agtaacgctt gttctatggt
    24841 ctgctgcagc atccgaatac gctgttccat acttgccgcg tagtcacgag tctttgcttt
    24901 ttcttgcgct aactcatagc tgatattcaa tgcggcaatg aagaccaact gttcagtatt
    24961 tgtgactcta gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt ccgctgcctg
    25021 attcaacgca tccctttggt caggcgggca gttcacacgc agtgaacggc caaaaatttg
    25081 gatatcgacg ggttgtgcag acatgccacc ttcctgctga ttgactgcgc tgccttcgtc
    25141 tttagaccca tggtctgcga aggggcgaca ctatagctac cctgatgaga agagacaagc
    25201 ccttttctgg tccaccaggg cccaaagtgg tagcatatca tgaatattcc tccctttgac
    25261 gacgaatgct tatgtctata cagaacgaaa tgcctggtta caacgaaatg aaccagtatc
    25321 tgaaccaaca agggacgggt ctgacccctg ccgagatgca tggtttaatc agtgggatga
    25381 tatgtggcgg taacgatgac agctcatggc tgccgctact tcacgacctg acgaacgaag
    25441 gcatggcttt cggtcatgag ctggcacagg cactgcgtaa aatgcactct gccaccagcg
    25501 atgccctgca ggatgacggc ttcctttttc agctttatct gcctgatggc gatgatgtca
    25561 gcgttttcga tcgggctgat gcactggcag gttgggtcaa tcacttcctg cttggtcttg
    25621 gcgttacgca accgaagctg gacaaagtga ccggcgaaac cggtgaagcc atcgacgatc
    25681 tgcgtaacat cgcgcagctg ggctacgacg aagacgaaga tcaggaagag cttgaaatgt
    25741 cgcttgaaga gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac gacaccttta
    25801 ctcatccgca accgaccgcg ccagaagtac aaaaaccgac tctacactaa aaacaaaaaa
    25861 cgtaaggaga gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg ccgtcaggcc
    25921 ctggtggagc aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc agaagtaaca
    25981 cgtagcgccg acagcgaata cccctatcgt cagaacagtg acttctggta cttcaccggc
    26041 tttaacgaac cggaagcggt gctggtgctg attaaaagcg atgacactca taaccacagc
    26101 gttctgttta accgcgttcg cgacctgacg gcggagatct ggtttggccg tcgcttaggc
    26161 caggatgccg cgccagagaa actgggcgtt gaccgcgcac tggcattcag cgaaatcaat
    26221 cagcaacttt atcaactact taacggcctg gatgtggttt accatgccca gggcgaatat
    26281 gcatatgctg atgaaatcgt gaacagtgcg ctggaaaaac tgcgtaaagg ttcgcggcaa
    26341 aatctcaccg caccggcaac gatgatcgac tggcgtcctg ttgttcatga aatgcgcctg
    26401 ttcaaatcgc cagaagagat tgccgtactc cgccgcgcgg gagaaatcac cgccatggca
    26461 catacacggg cgatggaaaa atgccgtccg ggaatgttcg agtaccatct ggaaggcgaa
    26521 attcaccacg aatttaaccg ccacggtgcg cgctatcctt cctataacac cattgtaggc
    26581 agcggtgaaa acggctgcat tctgcactac accgaaaacg agagtgaact gcgcgacggt
    26641 gacctggtgt tgattgacgc gggttgtgaa tacaaaggtt acgcgggcga tattacccgc
    26701 accttcccgg tcaacggcaa attcacccag gcccagcgtg aaatctacga cattgtgctg
    26761 gagtctctcg aaaccagcct gcgcctgtat cgtccgggaa cttccattct ggaagtcact
    26821 ggtgaagtgg tgcgcatcat ggttagcggc ctggtaaaac tcggcatcct gaaaggtaat
    26881 gttgatgaac tgatcgctca gaacgcccat cgtcctttct ttatgcatgg ccttagccac
    26941 tggttaggac tggatgtcca tgacgttggc gtttatggtc aggatcgctc gcgcattctg
    27001 gaaccgggga tggtactgac cgtagagcct ggtctgtata tcgcgccgga tgcggatgtg
    27061 ccagaacaat atcgcggtat cggcattcgc attgaagacg acattgtgat taccgaaacc
    27121 ggcaacgaaa acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga agcgttgatg
    27181 gctgctgcga gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg ggcgcgacgc
    27241 tggcgctggc tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg attgaagcga
    27301 ctgcgccaga gtcacatgct catccgggct ttgatggacg agcgatagcg ctggcggcgg
    27361 gtacctgtca gcaactggcg cgcatcggcg tctggcaatc tctggctgat tgcgcaaccg
    27421 ccatcactac cgtgcatgtc agcgatcgtg gtcatgctgg attcgtcacc ctcgccgcag
    27481 aagattacca actggcggcg ctggggcagg ttgtcgaatt gcataatgtc gggcaacggc
    27541 tgtttgcatt gctgcgaaaa gcacctggcg taatgctgca ttgccccgat cgcgtagcta
    27601 acgttgcccg tactcagagt catgttgaag tgacgctgga gagcggcgag atgctgacgg
    27661 gccgcgtgct gatagccgct gatggcaccc attcagcgtt agccaccgcg tgcggcgttg
    27721 attggcagca ggagccttac gaacaactgg cagtgattgc caacgttgct acttctgttg
    27781 cgcacgaagg ccgcgctttt gaacgcttca cgcaacatgg cccgctggcg atgttgccga
    27841 tgtctgacgg acgctgttcg ctggtctggt gtcatccact ggaacggcgc gaagaggtgc
    27901 tgtcgtggag cgacgagaag ttttgccgtg aactccagtc tgcctttggc tggcgactgg
    27961 ggcaaattac ccacgccggt aaacgcagtg cttatccgct ggcattaacc cgcgccgcca
    28021 gaccgattac ccatcgcact gtgctggtgg gcaatgcggc gcaaactctg cacccgattg
    28081 ccgggcaagg gtttaacctc ggtatgcgag atgtcatgag ccttgcggaa accctgactc
    28141 aggcgcatga gcgcggagaa gacatcggtg attacggcat attgtgccgc tatcagcagc
    28201 gtcgacagag cgatcgcgaa gcaaccatcg gcgtcacgga cagcctggta catctttttg
    28261 ccaaccgttg ggcaccgctg gttgtcgggc gcaacatcgg actgatgacg atggaattat
    28321 tcaccccggc acgcgatgtg ctggcgcagc gcaccctcgg ttgggtggcg cgttgaaggc
    28381 ggttttaagg aataaacaat gcaaagtgtt gatgtagcca ttgttggcgg cggcatggtg
    28441 gggctggcgg ttgcctgcgg cttacagggg agcggcttac gcgttgccgt actggagcag
    28501 cgcgtaccgg aacctctggc ggtagatgca ctaccacaac tgcgcgtttc ggctatcaat
    28561 gccgccagcg aaaaattact cacccgtctt ggcgtctggc aggacattct cgctcgtagg
    28621 gccagttgtt atcacggcat ggaagtgtgg gacaaagaca gttttggtca catctcgttt
    28681 gacgatcaaa gcatgggcta tagccatctt gggcacatcg ttgaaaactc agtgattcac
    28741 tacgcgctgt ggaacaaagc gcagcagtcg tcagatatta ctctgttggc ccccacagaa
    28801 ttacagcagg tcgcctgggg agaaaatgaa accttcctga cgctgaaaga tggcagcatg
    28861 ttaacggcgc gtctggtgat tggcgcggac ggtgctaatt cctggttgcg caacaaagca
    28921 gatattccgc tgactttctg ggattatcag catcacgcgc tggtagcgac cattcgcacg
    28981 gaagaaccgc atgatgcggt ggcgcggcag gttttccatg gcgaaggcat tctggccttt
    29041 ttaccgctta gcgatccgca tctttgctcg attgtctggt cactgtcgcc agaggaagcg
    29101 cagcggatgc agcaggcaag tgaagacgaa tttaatcgcg cgttaaatat cgcttttgat
    29161 aatcgccttg gcttatgcaa ggttgagagc gcgcgtcagg tgttcccgct gacggggcgt
    29221 tatgcgcgcc agtttgccgc acaccgtctg gcgctggtgg gcgacgccgc gcataccatt
    29281 cacccactgg cggggcaggg cgttaatctc ggatttatgg acgccgcaga gctgattgct
    29341 gaactgaaac ggttgcatcg tcagggtaaa gacatcgggc agtacattta cctgcgccgc
    29401 tatgaacgta gccgcaagca cagcgcggcg ctgatgctgg ctggtatgca gggttttcgc
    29461 gatctctttt ccggcgctaa tccggcgaaa aaactgctgc gtgatattgg tttgaaactg
    29521 gccgacacgc ttcctggcgt taagccgcaa cttatccgcc aggcaatggg attaaacgat
    29581 ttgcctgaat ggctgcgtta aaaatttctc ctctgttgtt tatttgatac ccatcacact
    29641 ttcatctccc ggttttttcg ccgggagatt ttcctcattt gaaataaact aatttcacct
    29701 ccgttttcgc attatatttt ctaataccat gattttttga tttagtgttt tttgacattt
    29761 ttttagctct taatattgtc ttattcaaat tgactttctc atcacatcat ctttgtatag
    29821 aaattggtgt attttttggt tttttattct gtcgcgattt ttgcattttt taaccataag
    29881 ctaatgtgat gatcaatttt accttatggt taacagtctg tttcggtggt aagttcaggc
    29941 aaaagagaac gattgcgttg gggaccggga gtggctccga tgctgggttt tgtggtgata
    30001 atttcaccat gaaaaagttg tcagccccgc ttattcaatg aggacaagat ggcacaacag
    30061 actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt ccacggctgg
    30121 atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc
    30181 ggaatgtttg atgtgtcgca tatgaccatc gtcgatctcc gcggcagccg cacccgggag
    30241 tttctgcgtt atctgctggc gaacgatgtg gcgaagctca ccaaaagagg caaagccctt
    30301 tactcgggga tgttgaatgc ctctggcggt gtgatagatg atctcatcgt ctactacttt
    30361 actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg
    30421 attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga cctttccatg
    30481 attgccgtgc aagggccgaa tgctcaggca aaagctgcca cactgtttaa tgatgctcag
    30541 cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc aggtgggcga tctgtttatt
    30601 gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg
    30661 gccgatttct ggcgtgcgct ggtggaagcg ggcgttaagc cctgcggtct gggggcgcgt
    30721 gacacgctgc gtctggaagc gggcatgaat ctttatggtc aggagatgga cgaaactatt
    30781 tctcctttag ccgccaacat gggctggact atcgcctggg aaccgacaga tcgtgacttt
    30841 atcggtcgtg aagccctgga agtgcagcgt gagcatggta cagaaaaact ggttggtctg
    30901 gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgctttac cgatgcgcag
    30961 ggcaaccagc atgaaggcat tatcaccagc ggtactttct ccccgacgct gggttacagc
    31021 attgcgctgg cgcgcgtgcc ggaaggtatt ggcgaaacgg cgattgtgca aattcgcaac
    31081 cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc gtaacggcaa agccgtcgcg
    31141 tgatttactt ttttggagat tgattgatga gcaacgtacc agcagaactg aaatacagca
    31201 aagaacacga atggctgcgt aaagaagccg acggcactta caccgttggt attaccgaac
    31261 atgctcagga gctgttaggc gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg
    31321 ttagcgcggg cgatgactgc gcggttgccg aatcggtaaa agcggcgtca gacatttatg
    31381 cgccagtaag cggtgaaatc gtggcggtaa acgacgcact gagcgattcc ccggaactgg
    31441 tgaacagcga accgtatgca ggcggctgga tctttaaaat caaagccagc gatgaaagcg
    31501 aactggaatc actgctggat gcgaccgcat acgaagcatt gttagaagat gagtaacggc
    31561 tttattcctc ttctgcggga gaggatcagg gtgaggaaaa tttatgcctc accctcactc
    31621 tcttcgtaag gagagaggtt cacaattcac tgcacgtttc aggaaccatc gctcatgaca
    31681 cagacgttaa gccagcttga aaacagcggc gcttttattg aacgccatat cggaccggac
    31741 gccgcgcaac agcaagaaat gctgaatgcc gttggtgcac aatcgttaaa cgcgctgacc
    31801 ggccagattg tgccgaaaga tattcagctt gcgacaccac cgcaggttgg cgcaccggcg
    31861 accgaatacg ccgcactggc agaactcaag gctattgcca gtcgcaataa acgcttcacg
    31921 tcttacatcg gcatgggtta caccgccgtg cagctaccgc cggttatcct gcgtaacatg
    31981 ctggaaaatc cgggctggta taccgcgtac actccgtatc agcctgaagt ctcccagggc
    32041 cgccttgaag cactgctcaa cttccagcag gtaacgctgg atttgactgg actggatatg
    32101 gcctctgctt ctcttctgga cgaggccacc gctgccgccg aagcaatggc gatggcgaaa
    32161 cgcgtcagca aactgaaaaa tgccaaccgc ttcttcgtgg cttccaatgt gcatccgcaa
    32221 acgctggatg tggtccgtac tcgtgctgaa acctttggtt ttgaagtgat tgtcgatgac
    32281 gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc tgttacagca ggtaggcact
    32341 accggtgaaa ttcacgacta cactgcgctt attagcgaac tgaaatcacg caaaattgtg
    32401 gtcagcgttg ccgccgatat tatggcgctg gtgctgttaa ctgcgccggg taaacagggc
    32461 gcggatattg tttttggttc ggcgcaacgc ttcggcgtgc cgatgggcta cggtggccca
    32521 cacgcggcat tctttgcggc gaaagatgaa tacaaacgct caatgccggg ccgtattatc
    32581 ggtgtatcga aagatgcagc tggcaatacc gcgctgcgca tggcgatgca gactcgcgag
    32641 caacatattc gtcgtgagaa agcgaactcc aacatttgta cttcccaggt actgctggca
    32701 aacatcgcca gcctgtatgc cgtttatcac ggcccggttg gcctgaaacg tatcgctaac
    32761 cgcattcacc gtctgaccga tattctggcg gcgggcctgc aacaaaaagg tctgaaactg
    32821 cgccatgcgc actatttcga caccttgtgt gtggaagtgg ccgacaaagc gggcgtactg
    32881 acgcgtgccg aagcggctga aatcaacctg cgtagcgata tcctgaacgc cgtggggatc
    32941 acccttgatg aaaccaccac gcgtgaaaac gtaatgcagc ttttcagtgt gctgctgggc
    33001 gataaccacg ggctggagat cgacacgctg gacaaagacg tggctcacga cagccgctct
    33061 atccagcctg cgatgctgcg cgacgacgaa atcctcaccc atccggtgtt taatcgctat
    33121 cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg agcgtaaaga tctggcgctg
    33181 aatcaggcga tgatcccgct gggttcctgc accatgaaac tgaacgccgc cgccgagatg
    33241 atcccaatca cttggacgga atttgccgaa ctgcacccgt tttgcccgcc ggaacaggct
    33301 gaaggttatc agcagatgat tgcgcagctg gctgactggc tggtgaaact gaccggttac
    33361 gacgccgttt gtatgcagcc gaactccggc gcacagggcg aatacgcggg cctgctggcg
    33421 attcgtcatt atcatgaaag ccgcaacgaa gggcatcgcg atatctgcct gatcccggct
    33481 tctgcgcacg gaactaaccc cgcttctgca catatggcag gaatgcaggt ggtggttgtg
    33541 gcgtgtgata aaaacggcaa catcgatctg actgatctgc gcgcgaaagc ggaacaggcg
    33601 ggcgataacc tctcctgtat catggtgact tatccttcta cccacggcgt gtatgaagaa
    33661 acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg gtcaggttta ccttgatggc
    33721 gcgaacatga acgcccaggt tggcatcacc tcgccgggct ttattggcgc ggacgtttcg
    33781 cacctcaacc tgcataaaac tttctgcatt ccgcacggcg gtggtggtcc gggtatggga
    33841 ccgatcggtg tgaaagcgca tctggcaccg tttgtaccgg gtcatagcgt ggtgcaaatc
    33901 gaaggcatgt taacccgtca gggcgcagtt tctgcggcac cgttcggtag cgcctctatc
    33961 ctgccaatca gctggatgta catccgcatg atgggcgcag aagggctgaa aaaagcaagc
    34021 caggtggcaa tcctcaacgc caactatatt gccagccgcc tgcaggatgc cttcccggtg
    34081 ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg
    34141 aaagaagaaa ccggcatcag cgagctggat attgccaagc gcctgatcga ctacggtttc
    34201 cacgcgccga cgatgtcgtt cccggtggcg ggtacgctga tggttgaacc gactgaatct
    34261 gaaagcaaag tggaactgga tcgctttatc gacgcgatgc tggctatccg cgcagaaatt
    34321 gaccaggtga aagccggtgt ctggccgctg gaagataacc cgctggtgaa cgcgccgcac
    34381 attcagagcg aactggtcgc cgagtgggcg catccgtaca gccgtgaagt tgcggtattc
    34441 ccggcaggcg tggcagacaa atactggccg acggtgaaac gtctggatga tgtttacggc
    34501 gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat accagtaatt cactgattcg
    34561 agtattttct aaaggcgctt cggcgccttt ttagtcagat gacaaagtac aaaagtgctc
    34621 agacagtccc ctcgcccctt tggggagagg gttagggtga ggggaacagg ccggcactgg
    34681 cgcgaatatt taccctcacc ccgaccctct ccctgaaagg gcgaggggga aaagcgtgcc
    34741 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    34801 tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg
    34861 caactgcatt actgttggcg caagaagggt atacggtggc ggttaattat cagcaaaacc
    34921 tccacgcggc gcaggaagtg atgaacttaa taatgcaagc cggtggcaaa gcattcgtgc
    34981 tccaggcgga tatcagcgac gaaaaccagg tcgttgcgat gtttacagca atcgatcagc
    35041 acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    35101 ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt
    35161 ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg
    35221 ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa tatgttgatt
    35281 acgcggcatc gaaaggggcg attgattcgt taaccaccgg actatcgctg gaagtcgccg
    35341 cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca
    35401 gcgcggcgag cctggacgcg tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg
    35461 acaggcagaa gaggtcgcgc aggccattgt ctggctacta agtgataaag cctcttacgt
    35521 cacgggaagt tttatcgatt tggcgggcgg gaaataaaac agggaagttg tctgaccgga
    35581 tgcaacaagt attgcatccg gtacttcatc gacttaaagc ttctcgccgt tgctggcaat
    35641 cacctctttg taccagttaa agctcttctt acgtgaacgc gacatatcgc cagtaccgtc
    35701 gtcatgttta ttcacataga taaagccgta gcgtttgctg tactgcccgg tagtgaatga
    35761 cacgcagtcg atgcagcccc acggtgtgta gcccatcaga tccacaccat cgtaagtcac
    35821 tgcttttttc atctcttcaa tatgggcgcg caggtagtca atgcggtagt cgtcgttgat
    35881 gctgccatct tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg
    35941 cctctgataa cgctcataca gttcgcaaag ggcatagcgc agacctactg gatcaatctg
    36001 ccagccccag tcagacgctt taacatacgg gttcggtacg ctgccttcaa aaccagagat
    36061 cgcatcgccg gtgccgcctt cggccttcac tgcgttggtc atgtaatagc tgaaaccaag
    36121 ataatcgcag gtgccttcgc gcagtacatc gagatcgccg tcttccattt tgatattaaa
    36181 tccgcgacgt tcccactcgt tcaatacata ggacgggtaa tagccgcata gctgcacatc
    36241 ggtaaagacg tagcgttcgc gcatcgactc ctgagcgaac atcacatcgt ccgggttaca
    36301 tgagtaggga tagagcggca ccatcgccag catacagccg actttcatct ccgggttaat
    36361 gcgacgcgcg gctttcactg ccagggcgct ggcgacaaac tggtgatgca gcacctggta
    36421 catcgtctct tccgggttgt catgctcggt atacaccacg ccggagcagc agtaaccaaa
    36481 cagcggcgca cgccagttac gctggttgtt tatttcgttg aaggtcatcc agtatttgac
    36541 tttatgcttg tagcgttcga ataccacttc cgcgaaacgg acaaagaaat caacgaattt
    36601 acggttggtc cagctaccgt attgctgcac cagatgcagc ggcatttcaa agtgggagag
    36661 ggttatcacc ggttcgatgt tgtatttcag cagttcatcg aacagagagt cgtaaaactt
    36721 cagcccttct tcgtttggct gagcttcatc accttttgga aaaatacgcg tccaggcgat
    36781 ggaagtccga aaacatttga agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg
    36841 accataaaaa tcaacggctt catggtttgg atagtatttt cctggcaaga cttctttggt
    36901 gatttcgcgc ggcacgccgt gtgcgccacc ggtcagaacg tcacaaatgc tcggtccttt
    36961 gccgcctttg ttccagccgc cttcgacctg atgagcggca acggcgccac cccataagaa
    37021 atctttcggt aaggtgagtt ttttcactat cataggctcc agatcagtca attcttacag
    37081 gaaatagtac catgcagcca aacgacatca cttttttcaa cgtttccagg atgacattct
    37141 ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga
    37201 tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc
    37261 aacctcaacc gtaacgctgg atacactgac ggaaaaacat gcagagcagg aaaatatgac
    37321 cctgcctgaa ctgaaaaagg tcattgctga catctatccc ggtcagacac aattttatat
    37381 gattgaattt aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa
    37441 tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca
    37501 ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctctggagt
    37561 aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca
    37621 acagcgtcag tcacggcatt gggctggtgt ttggtatcgt tgggctggtg ttgctactgg
    37681 ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg
    37741 gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg
    37801 caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct
    37861 acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta
    37921 tctggagcct agcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa
    37981 ttttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta atttatgaaa
    38041 tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt
    38101 cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg
    38161 gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg
    38221 cgtaatgggt atgcctttgt aggcctgata agacgcgtca gcgtcgcatc aggcatcgga
    38281 gcgcaaccgc cggatgcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt
    38341 gataaaggca tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat
    38401 gcctgatgcg atacagagat ttactcttcg agcgaatacg gcagcggctc gatatgcagt
    38461 gtattcgcat cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt attcatgacg
    38521 acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt accggtacga
    38581 cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctgaca
    38641 ctacctgcca gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc
    38701 atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg
    38761 gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa accagcttca
    38821 atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg
    38881 gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtgttca
    38941 aaccatagca aagtggtcgc gccttctttg actacctgtt tttctttcga aggcagttcg
    39001 ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc
    39061 tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag ttcagtcagc
    39121 tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc gcggaacaga
    39181 cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag gtgctgatct
    39241 tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg
    39301 ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca
    39361 gaagccgtag gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct
    39421 taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta
    39481 cgcgatctgt gccttctgcg aggcgactgc caaaaaaatg aagcccttga attgttgtga
    39541 attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagt cgaaaaacac
    39601 gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa
    39661 agcccgaatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc
    39721 gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct
    39781 gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga
    39841 tgcagaactg gaaataatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt
    39901 ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc
    39961 tggttgccgc tgttatttta ctcatgccct ggccgctcag ttacaccccg ttatggatgg
    40021 tgttactttc gctggtggta tttgattgcg ttcgcagcca gcggtgtatt aatgctcgcc
    40081 agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca
    40141 tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg
    40201 gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt
    40261 tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg
    40321 ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac
    40381 cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc
    40441 acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt
    40501 ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc
    40561 gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaataatt
    40621 ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt
    40681 tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc
    40741 ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt
    40801 ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata
    40861 acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc
    40921 acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg
    40981 agctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa
    41041 atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg
    41101 ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg
    41161 agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc
    41221 agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga
    41281 aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg
    41341 cgccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg
    41401 ctaccgggct gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc
    41461 agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg ttaggggaag
    41521 aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg
    41581 tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct
    41641 ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc
    41701 atgtgttgcg tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg
    41761 tgcagatgct actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta
    41821 ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa
    41881 cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggct
    41941 ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca
    42001 gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggtgtgt
    42061 tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagtggca
    42121 cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag
    42181 agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt actgatatta
    42241 cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca
    42301 ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga
    42361 aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa
    42421 gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc
    42481 ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc
    42541 agccgccgaa agacatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat
    42601 aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa cggcagactt
    42661 gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggag tacgcagtgc
    42721 gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga
    42781 aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg
    42841 tgatttcgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga tgcgctcgct
    42901 tggttgcagc aatatcgact acctggtacc aaaccgtttc gaagacggtt acggcttaag
    42961 cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca cggtggataa
    43021 cggtatttcc tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc cggttattgt
    43081 taccgatcac catttgccgg gcgacacatt acccgcagcg gaagcgatca ttaaccctaa
    43141 cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct
    43201 gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc
    43261 aattcctaac ctggcagaac tgctggatct ggtagcactg gggacagtag cggacgtcgt
    43321 gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg
    43381 aaagtgccgt ccagggatta aagcgctgct ggaagtggca aatcgcgatg cacaaaaact
    43441 agccgccagc gatttaggtt ttgcgctggg accgcgtctc aatgctgccg ggcgactgga
    43501 tgatatgtcc gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct
    43561 ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt
    43621 tgaagccctg accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct
    43681 ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa
    43741 agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg
    43801 ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact
    43861 ctaccccggc atgatgctca agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga
    43921 agaggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctggc
    43981 cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac
    44041 catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc
    44101 gctgtttgac ggtcatttcc gtctgctgca acagcggctg gtgggcgaac gtcatttgaa
    44161 ggtgatggtc gaaccggtcg gcggcggtcc actgctggat ggtattgctt ttaatgtcga
    44221 taccgctctc tggccggata acggcgtgcg cgaagtgcaa ctggcttata agctcgatat
    44281 caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta
    44341 gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca
    44401 ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg
    44461 cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca
    44521 agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaacgaac
    44581 ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc
    44641 tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag
    44701 ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg gaagaaaaac
    44761 tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgttacc
    44821 tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc
    44881 gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg
    44941 aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg
    45001 gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taaaagcccg tttgactccg
    45061 gcggtcgtcg ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata
    45121 ttgatatcga aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg
    45181 gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga
    45241 tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc
    45301 agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga
    45361 tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg
    45421 actcccgcat taaagatctg cgcaccgggg tagaaacccg caacacgcag gccgtgctgg
    45481 acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca
    45541 tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa
    45601 cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat tgccttcccg aacgatttcc
    45661 gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac
    45721 tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg
    45781 gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc
    45841 gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca
    45901 tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta
    45961 ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc
    46021 aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc
    46081 gtaacacctt taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc
    46141 gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc
    46201 gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg
    46261 aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta
    46321 acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct
    46381 acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc
    46441 tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg
    46501 gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg
    46561 acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc
    46621 acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag
    46681 aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg
    46741 cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc
    46801 gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc
    46861 tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt
    46921 acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc
    46981 gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga
    47041 tgcgtccggt gaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt
    47101 tatttaagct gggtaaatgc agataatcgt attctggctt cgcgatttgt cgcctgcatc
    47161 accatccacg gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa
    47221 tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt
    47281 gcggcaaata ccggacacac ttcattttcc acaatgcctc tcggatcggt ggcgcggtag
    47341 cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa
    47401 cggcggatca ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca aaccgagtta
    47461 gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgcccc
    47521 ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg
    47581 gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc
    47641 gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg
    47701 aaatgattaa ccaagagagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg
    47761 taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa
    47821 cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc
    47881 gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc
    47941 cacatacgta ctgtcctgac ttctaaatct ttatcagaga cggattcctt caccaggtca
    48001 gcatgatgct gatagccatt aaagaagacg ttcagcacaa cggcgctaag ggttgcgagc
    48061 ataatgccgc tatgtagcag cggttgcagt acggccggta acttagaaaa gaaatcgtga
    48121 gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag gttgtaacgg
    48181 ttggtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac catgccgaac
    48241 atcactagac cagcgccgcc cagcacaaat tgcggaatgg aggctaccag caccgccatt
    48301 tttggcacca tgccaaacag gattaatata attcccgaag aaatacacac ccagcggcta
    48361 tgaacgcgca tcacgctaac caggccaacg ttttgagaaa atgacgtgtg ggggaagctg
    48421 ttaaacgtgc cgcctatcat tgtccctacg ccatcgacac gcagcccgca aataatatcg
    48481 tgtgaagaga gtttacgacc gactatttca cccagtgcca ggaacatccc catcgactcg
    48541 ataaacacga tga
//
LOCUS       DAJVRL010000018        47954 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVRL010000018 DAJVRL010000000
VERSION     DAJVRL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.018"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OQN02_001024"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OQN02_001024"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1641910.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OQN02_001025"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OQN02_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1641911.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OQN02_001026"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OQN02_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1641912.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OQN02_001027"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OQN02_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1641913.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OQN02_001028"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OQN02_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1641914.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OQN02_001029"
     CDS             5377..5835
                     /locus_tag="OQN02_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1641915.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OQN02_001030"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OQN02_001030"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1641916.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OQN02_001031"
     tRNA            6887..6971
                     /locus_tag="OQN02_001031"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OQN02_001032"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OQN02_001032"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OQN02_001033"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OQN02_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1641917.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OQN02_001034"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OQN02_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1641918.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OQN02_001035"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OQN02_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1641919.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OQN02_001036"
     CDS             complement(10252..13995)
                     /locus_tag="OQN02_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1641920.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OQN02_001037"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OQN02_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1641921.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OQN02_001038"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OQN02_001038"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1641922.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OQN02_001039"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OQN02_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1641923.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OQN02_001040"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OQN02_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1641924.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OQN02_001041"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OQN02_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1641925.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OQN02_001042"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OQN02_001042"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1641926.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OQN02_001043"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OQN02_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1641927.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OQN02_001044"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OQN02_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1641928.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OQN02_001045"
     CDS             23392..23499
                     /locus_tag="OQN02_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1641929.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OQN02_001046"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OQN02_001046"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1641930.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OQN02_001047"
     CDS             complement(24538..25347)
                     /locus_tag="OQN02_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCS1641931.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OQN02_001048"
     CDS             complement(25351..25743)
                     /locus_tag="OQN02_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1641932.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OQN02_001049"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OQN02_001049"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1641933.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OQN02_001050"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OQN02_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1641934.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OQN02_001051"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OQN02_001051"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1641935.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OQN02_001052"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OQN02_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1641936.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OQN02_001053"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OQN02_001053"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1641937.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OQN02_001054"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OQN02_001054"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1641938.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OQN02_001055"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OQN02_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1641939.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OQN02_001056"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OQN02_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1641940.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OQN02_001057"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OQN02_001057"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1641941.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OQN02_001058"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OQN02_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1641942.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36789..39656
                     /locus_tag="OQN02_001059"
     CDS             36789..39656
                     /locus_tag="OQN02_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1641943.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="OQN02_001060"
     CDS             complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="OQN02_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCS1641944.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41903..42973
                     /gene="dhaK"
                     /locus_tag="OQN02_001061"
     CDS             41903..42973
                     /gene="dhaK"
                     /locus_tag="OQN02_001061"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCS1641945.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42984..43616
                     /gene="dhaL"
                     /locus_tag="OQN02_001062"
     CDS             42984..43616
                     /gene="dhaL"
                     /locus_tag="OQN02_001062"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCS1641946.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43627..45045
                     /gene="dhaM"
                     /locus_tag="OQN02_001063"
     CDS             43627..45045
                     /gene="dhaM"
                     /locus_tag="OQN02_001063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1641947.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45365..47062
                     /gene="treA"
                     /locus_tag="OQN02_001064"
     CDS             45365..47062
                     /gene="treA"
                     /locus_tag="OQN02_001064"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1641948.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47141..47580)
                     /locus_tag="OQN02_001065"
                     /pseudo
     CDS             complement(47141..47580)
                     /locus_tag="OQN02_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12035 a        12342 c        11747 g        11830 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggca cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcta
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccac ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccaa gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aagcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ttggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta cgacaactta gggaataact cgatataaac tcacctttgt
    36601 aagggggggg ttaaatatat attaattcct gtgttcctga ttttgcccgg taatagagaa
    36661 ttattctcag agaagctcat catgattgca ccatacagat atcatgatga taagaggata
    36721 ttattcattg acttaaacaa aatgacaaaa tccatagacc catagcctgg gtactatata
    36781 ggagtggtat gggcatcaaa caacacaatg ggaataccaa agccgatcgt ctcgctgaat
    36841 taaaaatccg ttcgccctca attcaactga taaaatttgg cgctattggt ttgaatgcaa
    36901 ttatcttttc ccccctgctg atagctgctg atacaggaag tcaatatggc accaatatta
    36961 ctattaatga tggtgacaga attacaggag ataccgccga tccatcagga aacctctatg
    37021 gtgtaatgac cccagcagga aacacgcctg gcaatatcaa cctgggtaat gatgtcaccg
    37081 tcaatgtcaa cgacgcctct ggatatgcaa aaggaatcat tattcagggc aaaaacagct
    37141 ccctgacagc taaccgactc acagtagatg ttgttggtca aacctctgcc atcggcatta
    37201 acttaattgg tgactatacc catgctgact taggcacagg cagcaccatt aagagtaacg
    37261 atgacggcat cattattggg catagctcaa cactaacagc cactcaattc accattgaaa
    37321 actcgaacgg tataggccta accatcaatg actatggcac cagtgtcgat cttggaagcg
    37381 gaagtaaaat caagaccgat ggaagtacag gtgtttatat cggtggtctc aacggcaata
    37441 acgccaatgg tgctgcgcgt tttacggcga cagacctgac aatcgatgtt cagggctaca
    37501 gcgccatggg gataaacgta cagaaaaact ctgttgtcga tctcggaaca aacagtacca
    37561 ttaaaaccaa tggcgataat gctcacggcc tctggagctt tggccaggtt agcgcgaatg
    37621 cactcactgt tgatgtaact ggagccgcgg ccaatggcgt cgaagttcgt ggtggtacaa
    37681 ccactatcgg tgcagatagc catatttctt ccgcgcaggg cggtggcctc gtcaccagta
    37741 gttcagacgc gacaatcaat ttttctggca cggcagcgca acgaaacagc atcttttccg
    37801 gcggttctta tggtgcctcg gcccagacgg caacggctgt tatcaacatg caaaataccg
    37861 atattacggt tgatcgtaat ggcagtctgg cgctgggttt gtgggcgctc agcggcggta
    37921 gaataaccgg agacagtttg gctatcaccg gcgcggcagg agccagaggg atttatgcca
    37981 tgaccaacag ccagatcgac ctcacgagcg atctggtcat tgatatgagt acacccgacc
    38041 agatggccat cgcaacgcaa catgacgatg gttatgccgc cagccgcatc aacgcctcgg
    38101 gtcgtatgct tatcaacggt agcgttcttt ccaaaggtgg gctaatcaat ctggatatgc
    38161 accctgggtc ggtttggaca ggttcctccc tcagcgataa tgtcaatggc gggaaactgg
    38221 acgttgcaat gaataacagc gtctggaacg taacaagtaa ttctaatctc gacacgctgg
    38281 cgctgagcca ttcaactgtc gattttgcca gccacgggtc aactgccggc acatttacca
    38341 cattaaacgt agagaacctg agcggtaaca gtacctttat tatgcgtgct gatgttgttg
    38401 gcgagggtaa tggcgttaat aatagagggg atttattgaa tatcagcggg agtagtgctg
    38461 gtaatcacgt attggctatc cgcaaccagg gcagcgaggc cacaacggga aatgaagttc
    38521 tgacagtggt aaaaaccact gacggcgcgg cctcgttcag cgcgtcttct caggttgagt
    38581 tggggggata tctgtacgat gtgcgtaaaa atggcactaa ctgggagctt tacgcttccg
    38641 ggacagttcc ggaaccgact cctaatcctg aacccacacc agctcccgct cagcctccca
    38701 tagtcaaccc cgatcctacg cctgaacccg ctcccacgcc taaacccacc acgaccgcag
    38761 atgctggcgg caattatctc aatgtcggtt acttattgaa ctatgttgaa aaccgtacgc
    38821 tgatgcaacg gatgggtgac ttgcgaaatc agagtaaaga cggtaatatc tggttgcgca
    38881 gttatggggg aagcctggac tcctttgcca gtggcaaact gagcggcttt gacatgggtt
    38941 acagcggtat ccagtttggt ggggataaac gtctctctga tgtaatgccg ttgtatgtcg
    39001 gtctgtatat tgactcaaca catgcatcgc cggactatag cggaggcgac ggtaccgcac
    39061 gttcagacta catgggaatg tacgccagtt acatggcaca aaacggtttt tacagcgatc
    39121 tcgttataaa agcatcgcgc cagaaaaata gtttccacgt actggacagt cagaacaacg
    39181 gcgttaacgc caacggcact gcgaatggaa tgagcatctc cctggaagcc gggcagaggt
    39241 tcaacctgtc ccctactggt tatgggttct atatagagcc gcaaacccag cttacataca
    39301 gccaccagaa tgagatggct atgaaggcga gtaatggcct caatatacat ctgaatcact
    39361 acgaatcgct gctggggcgt gccagcatga tactggggta tgacatcacc gcaggcaaca
    39421 gccagctgaa tgtctatgtg aagactggcg ctatccgcga gttttcaggg gataccgaat
    39481 atctgttgaa cgactcccgg gagaagtaca gtttcaaagg taatggctgg aataacggcg
    39541 tgggagtcag tgcacagtat aacaaacagc acacattcta tctcgaagcg gattacacgc
    39601 agggtaacct ctttgatcag aagcaagtca acggaggata tcgcttcagc ttttaagtgc
    39661 gggatagaca agcccttaaa cgatgctgaa aattgttagc gtgcgccgga atgaaataca
    39721 gatcactctg atgtatcaga aatcgtttct gtctttcata cccggcgctt aaactgccct
    39781 gcatcaatgc catgttgctt cattttccgc cacagcgtag tgcggccgat cccaagtaaa
    39841 gccgacattt cctgaatgcg accgcctgtg acctgggctg cgttaataat tgcctctttt
    39901 tcaacttccg caaatgacag actggtggaa aggcgggtgg cgctgacatc atctgtcgcc
    39961 tgctcggtaa acagatgttc aggtaaatca ctgacgcgaa tgcgcccgtt atcactactc
    40021 agagcaagat tctcgatgac gctgtaaagt tcaaaatcgt tgcctggcca tgcacaagaa
    40081 actaggcgag cgagggcatc gtcatcaatt ttcagccgcg tagagaagcg tttttcaaga
    40141 ctgcgtaatt tgttattcac cagcgccgga atgctgccac gccgcatacg cagaggcggg
    40201 atggtaattt caaatgcatg cagcgcgtaa tacagctggc gactaaaacg attttgttcc
    40261 accagcattg cgaggtccac ggtcgttgtt gcaatcactt tgacatcaat tggtattaaa
    40321 cgccgcgcat ccagtcgcgt gataaccccc tgcttgataa cctgcagcaa agcagactgt
    40381 aactccaccg ccagatattc aatcttttca agaaacagcg tgccgccgtg tgccagttcc
    40441 agccgactca gacggccatt ttcattgtcc gtgcgatcgc caccaataaa ttcttccgcc
    40501 agcgcagcat caccatataa ctcacaattg acggcgatat aaggacctgc agcacgctcg
    40561 ctttcattat gaattgcctg acttagcagt gccttgccca cgccctcttc tccacaaagc
    40621 aggacaggaa agctactgcg cgccgcctgg cgaccaaaat gaatcaagcg gcgggtttgc
    40681 ggatcgtcct gtggcatatg agcgaaggta tggctgactt ttcctaattg actggtcatt
    40741 aactgccgca tctgttccac aggatggagc aacaaaataa agctggttcc ctgcgtttct
    40801 attatcggtt taagggttat caccgcatca ataaactggt gctggctttc aaaggttgct
    40861 tctacgtgtt tgagcggatg tgcctgtttt attgcttgtt gcaatacggc gggtaacgtt
    40921 aagagttcag tgattgcccg tccctgactt gccgtcgcgt caaggcgcaa gacccgcgcc
    40981 gcctgggcat taataaattg caaattaccc tgctcgtccc agctaatcac gccatcatcc
    41041 atactttcta acagggcatt aagttgattt aaatgacggt tagtttcagc gagcaaactg
    41101 tccgtcagca gtaaattacc gacctcgcgg gcgattgcca acgtcaacgg caaatcagct
    41161 gcggtagttt gctcaaccgg acacgccagc gctattgttc ccgtcaatcg gcccttgctg
    41221 tcaaacaacg gcgttgcaca aaaggcccag ttccagagtg cctgtttgaa atgttgatcg
    41281 gccatcgttt tcacggcctg accagagata gccgctaacg atagcgcaca agtaccaata
    41341 attccctcgg cgcaatacgt gccgtcattg aatcccagtg cacttagctg ctgcaaggtt
    41401 tgcggatcgc cattacggct gagaatgcag gcggtttcat cgaggataaa cagcgcacac
    41461 tctcgcggtg ccatatattc ccaggcatct tccagcgcag cctgcccgag cgtcagcatc
    41521 gctttcttac gccgataaat agaagcaaat gtcacgccct gggcctgatg tggtacgttc
    41581 catgtctccc gtttcatcag cttattgcat cgctcccagg aggttgcaat taagggagat
    41641 atgccccgac catcgttgtt aaaagcgcca ctcatatccc gcatccctta tgacaaaagg
    41701 tgttccggct cgcaatttta agtattaata cacgtaaaag tttcaatatg gaacattcat
    41761 taaagcatat gtttcactat gaaacataat ttgggctaat tttcttttcc cttgcctgat
    41821 gcacaatgga ttcatcggca gtcagtggtc gccgtgtcgt tgaacatcat ccatgcccta
    41881 ccgtaattgc tggagcaaaa taatgaaaaa attgatcaat gatgtgcaag acgtactgga
    41941 cgaacaactg gcaggactgg cgaaagcgca tccatcgctg acactgcatc aggatccggt
    42001 gtatgtcacc cgagctgatg cccctgttgc aggaaaagtc gccctgctgt cgggtggcgg
    42061 cagcggacac gagccgatgc actgtggtta tatcggtcag gggatgcttt cgggggcctg
    42121 tccgggcgaa attttcacct caccgacgcc cgataaaatc tttgaatgct ccatgcaaat
    42181 tgatggcggc gaaggtgtac tgttgattat caaaaattac accggcgata ttcttaactt
    42241 tgaaacagcg accgagttac tgcacgatag cggcgtaaaa gtgaccactg tggtcattga
    42301 tgacgacgtt gcggtaaaag acagtcttta taccgccggg cgacgcggcg ttgccaacac
    42361 agtattaatt gaaaaactcg taggcgcagc ggcggagcgt ggcgactcac tggaggcctg
    42421 tgcggaactg gggcgtaagt tgaataatca aggccactca ataggtatcg ctctcggtgc
    42481 ctgtaccgtt cctgccgcgg gcaaaccttc ttttaccctt gcggataatg agatggagtt
    42541 tggcgtcggc attcatggtg agccaggtat tgaccgccgc cctttctctt cccttgatca
    42601 aaccgtcgat gaaatgttcg acaccctgct ggaacatggc tcataccatc gcactttgcg
    42661 tttctgggat tatcaacaag gcagctggca ggaagaacca caaaccaaac aaccgctcca
    42721 gtctggcgat cgggtgattg cactggttaa caatcttggc gcaactccgc tttctgagct
    42781 gtacggcgtc tataaccgcc tgaccacacg ttgccagcaa gcgggattga ctatcgaacg
    42841 taatttaatt ggcgcgtact gcacctcact ggatatgacc ggtttctcaa tcaccttact
    42901 gaaagttgat gacgaaacgc tggctctctg ggacgccccg gtccacaccc cggcccttaa
    42961 ctggggtaaa taaggagaaa gcaatgtcac tgagcagaac tcaaattgtt aactggctca
    43021 ctcgttgtgg cgatattttc aacaccgaga gcgagtatct taccgggctg gatcgcgaga
    43081 ttggcgacgc tgaccacggg ctaaatatga accgaggctt tagcaaagtg gtggaaaaac
    43141 tccctgctat cgcagataaa gatatcggtt tcattctcaa aaataccggt atgacgctgc
    43201 tttccagcgt cggtggtgcc agtggtccgc tgttcggtac cttctttatc cgcgccgcac
    43261 aggcgaccca ggcacggcaa agcctgacac tggaagagct ttatcagatg ttccgcgatg
    43321 gcgcggacgg cgtaatcagt cgcgggaaag ccgaacctgg cgataaaacc atgtgtgatg
    43381 tgtgggtgcc ggtggtggaa tcgttacgtc agtccagcga gcaaaatctc tctgttccgg
    43441 tggcgctcga agctgccagt agcatcgccg aatccgctgc acaaagtacg attacgatgc
    43501 aagcccgcaa aggccgcgcc agttatctcg gtgaacgcag tattggtcac caggatcccg
    43561 gcgcgacctc ggtgatgttt atgatgcaaa tgttggcgtt agccgcaaaa gagtaaggaa
    43621 ttggtgatgg taaacctggt catagtttca catagcagcc gactgggaga aggtgtcggt
    43681 gaattagccc gtcagatgtt aatgagtgat agttgtaaaa tcgccattgc cgcgggaatt
    43741 gacgatccac aaaatcccat tggtaccgat gccgtcaaag tgatggaggc catcgaatct
    43801 gttgctgatg ccgaccatgt gctggtcatg atggatatgg gtagcgcatt attgagtgct
    43861 gaaactgcgc tggaattgct ggctcccgag atcgccgcaa aagtacgttt gtgtgctgcg
    43921 ccgttggtcg aaggtacact ggcagcaacg gtcagcgcgg cctcgggggc ggatatcgac
    43981 aaagttatct ttgacgctat gcatgcgctg gaagccaaac gtgaacaact gggtttaccg
    44041 tcctccgaca ctgaaatctc tgacacatgt cctccgtacg atgaagaagc ccgttctctg
    44101 gcggtggtca taaaaaaccg taacggcctg catgtacgtc cggcctcccg gctggtttat
    44161 accttatcga catttaatgc cgatatgttg ctggaaaaaa acggcaaatg cgtcacacca
    44221 gagagtatta accagattgc gttactacaa gttcgctata acgatacgct gcgcctgatt
    44281 gcgaaagggc cagaagctga agaggcactg atcgctttcc gtcacctggc tgaagataac
    44341 tttggtgaaa cggaggaagt cgctccacct actctgcgtc ccgttctgcc tgtttcgggt
    44401 aaagcctttt attatcaacc agttttatgt acggtacagg caaaatcaac cctgaccgcg
    44461 gaagaagaac aagatcgatt acgccaggct attgacttca cgttattaga tctgatgacg
    44521 ttaacagcga aagcagaagc cagcgggctt gacgatattg ccgcaatctt ttctggtcac
    44581 catacactgt taggtgatcc ggaactgctg gcggcggcaa gcgaactcct tcagcatgaa
    44641 cattgcacgg cagaatatgc ctggcagcaa gttcttaaag aacttagcca gcaataccag
    44701 caactggatg atgaatatct acaagctcgc tatattgatg tggacgatct tctgcatcgc
    44761 accctggtcc acctgaccca aacgaaagaa gaactcccgc agtttaactc gccaactatt
    44821 ctactggcgg agaacattta tccttccaca gtactgcaac tggatccggc ggttgtaaaa
    44881 ggtatctgcc ttagcgccgg aagtccggta tcccacagcg ccctaatcgc ccgtgaactg
    44941 gggattggct ggatttgcca gcagggtgag aaactgtatg cgatacaacc agaagaaacg
    45001 ctaacgctgg acgttaaaac gcaacgtttc aaccgtcagg gttaagccac cgttcggatg
    45061 gcatcgttct gatgtcatcc ggcaatttac ccagcactga tgttttttaa ctacctgtta
    45121 tattttttac caatttatga tgatttttct tcgctaatcg cgaaccaaat gtcgctggct
    45181 ggctaataaa cagacgttat ttgatcactc tatttcgccg ttatttatcc ctttaattcc
    45241 cttttctaaa atgcctgacc gttcgcagaa tgagatttcg atcatgcagc tagtgcgatc
    45301 ctgaactaag gttttctgat acttgaatac cgttttattc ggtttcgcca aaggagaatg
    45361 attgatgaaa tcccccgcac cttctcgccc gcaaaaaatg gcgttaattc cagcctgtat
    45421 ctttttgtgt ttcgctgcgc tatcggtgca ggcagaagaa acaccggtaa caccacagcc
    45481 gcctgatatt ttattagggc cgctgtttaa tgatgtgcaa aacgccaaac tttttccgga
    45541 ccaaaaaacc tttgccgatg ccgtgccgaa cagcgatccg ctgatgatcc ttgctgatta
    45601 tcggatgcag caaaaccaga gcggatttga tctgcgccat ttcgttaacg tcaatttcac
    45661 cctgccgaaa gaaggcgaga aatatgttcc gccagagggg cagtcactgc gcgaacatat
    45721 tgacggactt tggccggtat taacgcgttc taccgaaaac accgaaaaat gggattctct
    45781 gttaccgctg ccggaacctt atgtcgtgcc gggcggacgt tttcgcgagg tatattactg
    45841 ggacagttac ttcaccatgt taggacttgc cgaaagtggt cactgggata aagtcgcgga
    45901 tatggtggcc aattttgctc atgaaataaa cacttacggt catattccca acggcaaccg
    45961 cagttactat ttaagccgct cgcaaccgcc cttctttgcc ctgatggtag agttactggc
    46021 gcagcatgaa ggcgatgccg cgttgaagca atacctgccg caaatgcaaa aagaatatgc
    46081 ttactggatg gacggtgttg aaaacctgca agccggacaa caggaaaaac gcgttgtaaa
    46141 acttcaggat ggtacccttc tcaaccgcta ctgggacgat cgcgatacgc cacgaccaga
    46201 gtcatgggtg gaagatattg ccaccgccaa aagcaatccg aatcgacctg ccactgaaat
    46261 ttaccgcgac ctgcgctctg ccgctgcgtc tggctgggat ttcagctcgc gctggatgga
    46321 caacccgcag cagttaaata ccttacgcac caccagcatc gtaccggtcg atctgaacag
    46381 cctgatgttt aaaatggaaa aaatcctcgc ccgcgccagc aaagctgcag gagataacgc
    46441 gatggcaaac cagtacgaaa cgctggcgaa tgcccgtcaa aaagggatcg aaaaatacct
    46501 gtggaacgat caacaaggct ggtatgccga ttacgacctg aaaagtcata aagtgcgcaa
    46561 tcagttaacc gcggccgccc tgttcccgct gtacgtcaat gcggcagcga aagatcgcgc
    46621 caacaaaatg gcgacggcga cgaaaacaca tctgctgcaa cccggcggcc tgaacaccac
    46681 gtcggtgaaa agtgggcaac aatgggatgc gccaaatggc tgggcaccgt tacagtgggt
    46741 cgcgacagaa ggattacaaa actacgggca aaaagaggtg gcgatggaca ttagctggca
    46801 cttcctgacc aatgttcagc acacctatga ccgggagaaa aagctggtgg aaaaatatga
    46861 tgtcagcacc accggaacgg ggggcggcgg tggcgaatat ccattacagg atggctttgg
    46921 ctggaccaat ggcgtgacgc tgaaaatgct ggatttgatc tgcccgaaag agcaaccgtg
    46981 tgacaatgtt ccggcgacgc atccgaccgt taagtcagca acgacgcaac cctcaaccaa
    47041 agaggcacaa cccacacctt aaccagcgct tactccgaca acgtcattcg gcttctccgg
    47101 atggcgttgt cggcagataa caccatatct atggtttttt ctacacagac ggtttttcaa
    47161 gtcctggcgg gaagtattgc gcaaatgttt gccatacgtg gttgccgaag atagcgtcat
    47221 ttctacgggt aagtgtttca ctttcatcac ccggcgattt acgccgacgt tggctatata
    47281 aagcatcaat tgctaccgaa tattgttacg gtgttcactc atcaacgttt ttttgctttc
    47341 tggagttaaa ttaataatca cataatccag acactccaga gaatttagca accaaaaaaa
    47401 gaattcagac cattcctctt cgcgtaactg aagcattgcc gaacgggtat tttggcttaa
    47461 tctgccagaa ggattgatca attctaaatg ttcaataagc gcagccaaaa ccgcctcact
    47521 atccgcccca tttttaacgc ctgactggct actgtctaaa atatttattt tctgaatcac
    47581 accaaaaaca acgaaacaat aattaaaaaa ggattctgtt gttgttaccg ggaaccgact
    47641 tatttcgctc agaaagtcgt tcaaccagct ccgcagtgat aagattcgct gatgatacag
    47701 tgtatcctca ctgcgtgctg gaggtgccag tataatatgt tgatcaatac agtatttatt
    47761 gtgttcagtc gtttcctgat gcgtttggtt ctctgcggat tgtcgctggc gaaataaatg
    47821 gcttgcggta aatgccgtta cagagcagcg caacgcgtca attccttcgg agtgcccggc
    47881 ttttggcgag agttccggtt ttatgcgtcc aggtagcgga aatttcaccc gaatgagaaa
    47941 tgcattaata aaat
//
LOCUS       DAJVRL010000019        47688 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVRL010000019 DAJVRL010000000
VERSION     DAJVRL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.019"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(1..58)
                     /locus_tag="OQN02_001066"
     tRNA            complement(1..58)
                     /locus_tag="OQN02_001066"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31..33),aa:Val,seq:tac)
     gene            complement(118..193)
                     /locus_tag="OQN02_001067"
     tRNA            complement(118..193)
                     /locus_tag="OQN02_001067"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Lys,seq:ttt)
     gene            complement(196..271)
                     /locus_tag="OQN02_001068"
     tRNA            complement(196..271)
                     /locus_tag="OQN02_001068"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Val,seq:tac)
     gene            complement(408..483)
                     /locus_tag="OQN02_001069"
     tRNA            complement(408..483)
                     /locus_tag="OQN02_001069"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(448..450),aa:Lys,seq:ttt)
     gene            complement(648..1439)
                     /gene="cpoB"
                     /locus_tag="OQN02_001070"
     CDS             complement(648..1439)
                     /gene="cpoB"
                     /locus_tag="OQN02_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1641949.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1449..1970)
                     /gene="pal"
                     /locus_tag="OQN02_001071"
     CDS             complement(1449..1970)
                     /gene="pal"
                     /locus_tag="OQN02_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1641950.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2005..3297)
                     /gene="tolB"
                     /locus_tag="OQN02_001072"
     CDS             complement(2005..3297)
                     /gene="tolB"
                     /locus_tag="OQN02_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1641951.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3430..4671)
                     /gene="tolA"
                     /locus_tag="OQN02_001073"
     CDS             complement(3430..4671)
                     /gene="tolA"
                     /locus_tag="OQN02_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1641952.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4736..5164)
                     /gene="tolR"
                     /locus_tag="OQN02_001074"
     CDS             complement(4736..5164)
                     /gene="tolR"
                     /locus_tag="OQN02_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1641953.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5168..5860)
                     /gene="tolQ"
                     /locus_tag="OQN02_001075"
     CDS             complement(5168..5860)
                     /gene="tolQ"
                     /locus_tag="OQN02_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1641954.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5857..6261)
                     /gene="ybgC"
                     /locus_tag="OQN02_001076"
     CDS             complement(5857..6261)
                     /gene="ybgC"
                     /locus_tag="OQN02_001076"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1641955.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6411..6704)
                     /gene="ybgE"
                     /locus_tag="OQN02_001077"
     CDS             complement(6411..6704)
                     /gene="ybgE"
                     /locus_tag="OQN02_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1641956.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6704..6817)
                     /gene="cydX"
                     /locus_tag="OQN02_001078"
     CDS             complement(6704..6817)
                     /gene="cydX"
                     /locus_tag="OQN02_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1641957.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6832..7971)
                     /gene="cydB"
                     /locus_tag="OQN02_001079"
     CDS             complement(6832..7971)
                     /gene="cydB"
                     /locus_tag="OQN02_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1641958.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7987..9555)
                     /gene="cydA"
                     /locus_tag="OQN02_001080"
     CDS             complement(7987..9555)
                     /gene="cydA"
                     /locus_tag="OQN02_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1641959.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10404..13037)
                     /gene="mngB"
                     /locus_tag="OQN02_001081"
                     /pseudo
     CDS             complement(10404..13037)
                     /gene="mngB"
                     /locus_tag="OQN02_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(13055..14971)
                     /gene="mngA"
                     /locus_tag="OQN02_001082"
     CDS             complement(13055..14971)
                     /gene="mngA"
                     /locus_tag="OQN02_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1641960.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15141..15863
                     /gene="mngR"
                     /locus_tag="OQN02_001083"
     CDS             15141..15863
                     /gene="mngR"
                     /locus_tag="OQN02_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1641961.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15966..16835)
                     /gene="sucD"
                     /locus_tag="OQN02_001084"
     CDS             complement(15966..16835)
                     /gene="sucD"
                     /locus_tag="OQN02_001084"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1641962.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16835..18001)
                     /gene="sucC"
                     /locus_tag="OQN02_001085"
     CDS             complement(16835..18001)
                     /gene="sucC"
                     /locus_tag="OQN02_001085"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1641963.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18095..19312)
                     /gene="odhB"
                     /locus_tag="OQN02_001086"
     CDS             complement(18095..19312)
                     /gene="odhB"
                     /locus_tag="OQN02_001086"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1641964.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19327..22128)
                     /gene="sucA"
                     /locus_tag="OQN02_001087"
     CDS             complement(19327..22128)
                     /gene="sucA"
                     /locus_tag="OQN02_001087"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1641965.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22429..23145)
                     /gene="sdhB"
                     /locus_tag="OQN02_001088"
     CDS             complement(22429..23145)
                     /gene="sdhB"
                     /locus_tag="OQN02_001088"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1641966.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23161..24927)
                     /gene="sdhA"
                     /locus_tag="OQN02_001089"
     CDS             complement(23161..24927)
                     /gene="sdhA"
                     /locus_tag="OQN02_001089"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1641967.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24927..25274)
                     /gene="sdhD"
                     /locus_tag="OQN02_001090"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24927..25274)
                     /gene="sdhD"
                     /locus_tag="OQN02_001090"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1641968.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25268..25672)
                     /gene="sdhC"
                     /locus_tag="OQN02_001091"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25268..25672)
                     /gene="sdhC"
                     /locus_tag="OQN02_001091"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1641969.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26053..26160
                     /locus_tag="OQN02_001092"
     CDS             26053..26160
                     /locus_tag="OQN02_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641970.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26366..27649
                     /gene="gltA"
                     /locus_tag="OQN02_001093"
     CDS             26366..27649
                     /gene="gltA"
                     /locus_tag="OQN02_001093"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1641971.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28040..28606
                     /locus_tag="OQN02_001094"
     CDS             28040..28606
                     /locus_tag="OQN02_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1641972.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28657..31116
                     /locus_tag="OQN02_001095"
     CDS             28657..31116
                     /locus_tag="OQN02_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1641973.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            31131..31862
                     /locus_tag="OQN02_001096"
     CDS             31131..31862
                     /locus_tag="OQN02_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1641974.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31859..32935
                     /locus_tag="OQN02_001097"
                     /pseudo
     CDS             31859..32935
                     /locus_tag="OQN02_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            33087..34133
                     /locus_tag="OQN02_001098"
     CDS             33087..34133
                     /locus_tag="OQN02_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1641975.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34130..34921)
                     /gene="nei"
                     /locus_tag="OQN02_001099"
     CDS             complement(34130..34921)
                     /gene="nei"
                     /locus_tag="OQN02_001099"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1641976.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34957..35691)
                     /gene="pxpA"
                     /locus_tag="OQN02_001100"
     CDS             complement(34957..35691)
                     /gene="pxpA"
                     /locus_tag="OQN02_001100"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1641977.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35681..36613)
                     /gene="pxpC"
                     /locus_tag="OQN02_001101"
                     /pseudo
     CDS             complement(35681..36613)
                     /gene="pxpC"
                     /locus_tag="OQN02_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36607..37263)
                     /gene="pxpB"
                     /locus_tag="OQN02_001102"
     CDS             complement(36607..37263)
                     /gene="pxpB"
                     /locus_tag="OQN02_001102"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1641978.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37286..38029)
                     /gene="ybgI"
                     /locus_tag="OQN02_001103"
     CDS             complement(37286..38029)
                     /gene="ybgI"
                     /locus_tag="OQN02_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1641979.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38300..39781
                     /gene="dtpD"
                     /locus_tag="OQN02_001104"
     CDS             38300..39781
                     /gene="dtpD"
                     /locus_tag="OQN02_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1641980.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39931..41349)
                     /gene="phrB"
                     /locus_tag="OQN02_001105"
     CDS             complement(39931..41349)
                     /gene="phrB"
                     /locus_tag="OQN02_001105"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1641981.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41346..41855)
                     /locus_tag="OQN02_001106"
     CDS             complement(41346..41855)
                     /locus_tag="OQN02_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1641982.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41984..42271
                     /locus_tag="OQN02_001107"
     CDS             41984..42271
                     /locus_tag="OQN02_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCS1641983.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42326..42706)
                     /locus_tag="OQN02_001108"
     CDS             complement(42326..42706)
                     /locus_tag="OQN02_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1641984.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42709..47373)
                     /locus_tag="OQN02_001109"
                     /pseudo
     CDS             complement(42709..47373)
                     /locus_tag="OQN02_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(47392..47595)
                     /locus_tag="OQN02_001110"
                     /pseudo
     CDS             complement(47392..47595)
                     /locus_tag="OQN02_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11295 a        13107 c        11605 g        11681 t
ORIGIN      
        1 tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca gctgagctaa
       61 tcacccgata ctgcgctgga tactacaaac tacttccacc gaacccaccg taattagtgg
      121 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact
      181 gagctaacga cccggtggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag
      241 ggaggtgctc tcccagctga gctaatcacc cgacactacg ctggatacta caactaacta
      301 tgttagtggt gggtcgtgca ggatgactcg acttcgtctc gcccttcggc gccgttgctg
      361 atgcaacgtt atccttcacg ttttactacc gcttccacct tagcgattgg tgggtcgtgc
      421 aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact gagctaacga
      481 cccacttttt cgttgctttc ggtttgtttg atatcccgtg gcaacggcgg catatattac
      541 tgatttcaga cttgagcgca acaaaaattt cgatgcagat cactcaactg cttatgattc
      601 gcacgacacg accagaaata atgcgacttc tggtcgtgtg ttatgcatta catcgcgttc
      661 agacgttttt gtgcctgttt agcgccatca gtaccagggt atttactgat aacctgctgg
      721 tatacggctt tcgcttttgc ggtgtcacct ttgtcctgca tgatgacgcc gactttaaac
      781 atcgcatcag cagcctttgg tgacttcgga tagtttttca ctaccgaagc aaaatagtac
      841 gccgcatcat cttttttacc cttgttgtag tttaactgac ccagccaata attggcgttt
      901 ggcaggtaag ttgaatcagg gtaatttttg atgaaattct gaaatgccac cattgcgtca
      961 tcctggcggg atttatcctg caccagcgca atagctgcat tgtaatccgt gtttgcatcg
     1021 ccgcttttca ccggcgcgcc agcattcgca gtaccagcat cagctgtcgg cgtcgttgat
     1081 gccgtcgcac cgctttgatc gctgctggtt gattgcgccg ctgcaccacc gctgctgagg
     1141 ctgtcgatct gcaacaggat ctgcttttgc cgctccacga cctgattcag ttgatactga
     1201 ttttcctgaa tctgaccacg cagggaatca atatcggatt gattatcaga aagttgttgc
     1261 tggagttggg ttaaaagctg gctgtgagca ttagaaatac gctcaagttg agtgacgcgg
     1321 tcttcgaccg agcctgagcc gacactactg attggtgcct gagcaaaagc ggcccagggg
     1381 gccgctatac caaccagtaa cgacagactc aatagttgat gtctgaagtt actgctcatg
     1441 caattctctt agtaaaccag taccgcacga cggtttttgg agtatgccgc ttcgtcatga
     1501 cccagtactg caggtttttc tttaccgtaa gaaacgatgg agatctggtc tgcagaaacg
     1561 cctttaccct gcaggtacat cttaacggcg ttcgcacgac gttcacccag ggagatgttg
     1621 tattccggag taccacgttc gtccgcgtga ccttctacgg tgactttgta agacgggttg
     1681 ctacgcagga agtttgcatg tgcatccagc atttgagcga agtcagaacg gatatcgtac
     1741 ttgtccagat cgaagtaaac gatgttgttc tgctgcagct gttgcatttg cagacgagcc
     1801 tgctcttcgg aagacatgtt gccgttgccg ccgttcgcat ccataccagt gccggcaccc
     1861 agcatgcctt cgctgccgtc attgctggcg ttcttgttgg aagaacatgc cgcaattgcc
     1921 ataacaggca gagcaatcat cagccctttc agcactttgt tcagttgcat ttcaatgatt
     1981 cctttactat tcaattaatt attatcacag atacggcgac caggcaggga atttgacctg
     2041 tccatcagtt gccggaagac gcgctttgaa acgcccatct gtagaaacca aattcagcac
     2101 ggatcccatc ccctgagaag agctgtagat taccatagtg ccgttaggtg ccagacttgg
     2161 cgtttcatcc aggaacgtgg acgacagaac ttgtacgcct cccgttgcca gatcttgttt
     2221 ggcaatgtgc tgctgcccac cattggagct gaccattacc ataaatttac cgtcgctgct
     2281 gacatccgca tcctggttct gcgaaccttc ccaggtaata cgttgtggcg caccgccgtt
     2341 gatattcact ttataaacct gcggacgacc ggcctggtca gaagtaaatg ccaggttctg
     2401 gctatccggg aaccaggtcg gttcggtatt gttactgcga ccatcagtca cctggcggat
     2461 ctgaccagaa gccaaatcca ttacgtacag gttcagacta ccggttttcg acaaggcgaa
     2521 tgccagtttg ctgccgtctg gcgagaatgc aggtgcaccg ttgtgacgcg ggaatgaagc
     2581 cacctgacgt acagcgccat ttgccagcgt ctgaataacc agcgcggaac gaccgctttc
     2641 gaaggtcaca taagccagtt tagaaccgtc tggtgaccac gccggagaca tcagcggctg
     2701 cggtgaacgg tgaacgacaa actggttgta accgtcatag tcagatacgc gcagttcata
     2761 cgggaactga ccgccgttgg tctgaacaac gtaggcaata cgggtacgga acgcaccttt
     2821 aatgccggtc agtttttcaa acacttcatc actggcggta tgaccagcat aacgcagcca
     2881 ctgcttgttc actttgtacg agttctgagc aagtacagta cccggtgcgc cgccagtgtc
     2941 aacaagttga taagcaacat tgtaagaacc atccggattc ggagtgacct gaccgacaac
     3001 tacagcgtca atgcccagtg cggaccatgc agctggttgt acttcctgcg cactacccgg
     3061 ctgctgtggc agacgagcgc gatctaacgg attaaattta ccgctgttac gcaagtcagc
     3121 agcaacgatg ccgccaatat cttcaggtgc cgcaccaggc cccgcccact ggaaaggaac
     3181 aacaccaata ggacgaccgg aatctacacc gctgtcgatc acaatgcgga cttcagcatg
     3241 cagaactgat gcccacagta tgagaaaacc aaatgctact cgtaatgcct gcttcatcat
     3301 atctccctta tctggaccga tggcccacga taatttagca gaatgttaac aaactaaaat
     3361 acacaaaact accagaaccc cgtggcaacc ggtgcctgat gttgaccgtc cgaacagtca
     3421 acatcgcgat tacggtttga agtccaatgg cgcgtttttg aacacttcat atactgcctg
     3481 gcttggtggt ttcgggatct tcgcaagttt agctgctgcc aacgcagcct gacaaagtgc
     3541 gggatcgcca ccttcaggtt tgatatccag taacatacca tcgggtgcca gttttatgcg
     3601 cagcgtacag gttttgcctg cataggacga tgcgtcatag aacttacttt cgatagcaga
     3661 tttaatctgc ccggcatagt tattgatatc ggcccctgat gcgccattgt ttttagtatt
     3721 accactcccg gcaggcgaag cattgttccc tttcgcccct ccccccgttt tcggtgcatt
     3781 cttaccagag cttagctcac cgaaaatatc atctgcctct gcggcagctt ttgctgcagc
     3841 ggctttttca gctgctgctt ttgccgctgc cgcttttttg tctgcggcgg ctttttctgc
     3901 cgctgctttc ttatcagctg ctgccttatc agcagccgct ttttctgctg cttttttgtc
     3961 ggctgcagct ttctctgctg ccgctttctt atcagctgca gccttttcag cagccgcttt
     4021 cttctcagct tctgccttgg ctttttcagc agcttcagtt gctgctttct ttcttgcttc
     4081 agctgcagct gcttctgccg cttccgcttt cttcttcagt gccgcagcgg ctacctcggc
     4141 ttttttctgc gcttcggctg cggctttggc ggcttctgct tctgcttttt tcttcgcgtc
     4201 tgcagccgct ttcttcgctg cttcttccgc agctttagca tctgccgccg ctttcgctgc
     4261 cgccacttca gcttgcttct gctttaactc ggcctgtttt gcggcttctt cagcctgctt
     4321 tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc
     4381 cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc ttcatcttgc gctgttcatc
     4441 agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa ctaccgcacc
     4501 tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg aagcttctat
     4561 attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca gcactgctga
     4621 aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt
     4681 ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca gatgtttaga
     4741 taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag ttcagtgctt
     4801 taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc gttttcgggt
     4861 tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa cgctccaggc
     4921 gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca atcactggcg
     4981 gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg acctccacgc
     5041 tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc agtacgtcca
     5101 gcaacggaac aatgttgatt tcggacttga gatcgcgacg acctcgtcca cgcgctctgg
     5161 ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg
     5221 taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc tggttgagac
     5281 ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgccgtc gcaatcaacg
     5341 cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc ccgagggcga
     5401 taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg ctgatggagc
     5461 caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg ttcatggaga
     5521 tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta ttggcacgat
     5581 gcaggcgcac aaactctttg aacccgctgt aaaagatttg ttccgaacca gtcagattat
     5641 cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac cagaatttat
     5701 cttcaaacgc ttcggcttca cgcgccgctg cgttaagaat acgggttcgc tggataataa
     5761 tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta accagaaggc
     5821 tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact ccgcgacaat
     5881 agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa ccagaacctc
     5941 tgcttcattc agcaaagtat tctcggcgtt gacaatacgt tgcgtgaaaa ccaaagaggt
     6001 gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc
     6061 gtaatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg
     6121 ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta
     6181 actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc gaaccggcca
     6241 tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta gagcttttaa
     6301 acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta agggcgcgta
     6361 aaaatttatg ggcctggagc ggcccataat tttttaaaga gaattctgat ttaaaagaag
     6421 aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa aatcccttgc
     6481 caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca gacggcccac
     6541 atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa acgagatggg
     6601 tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc ccgcaggggg
     6661 cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg tcttcttgac
     6721 cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca aacgaacagg
     6781 caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta cttagtacag
     6841 agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt tccagtaaca
     6901 ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg tcatgacgtt
     6961 aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca tcatggtgct
     7021 ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc aggccagcgt
     7081 cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca ggatggtcag
     7141 cagcagcaga accacaccca gtgccggaat agcccacaga attggcgtgt tgttgaagtt
     7201 aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag aggctgcgta
     7261 atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc atacgccagc
     7321 cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac gggtacgcag
     7381 gtgcagttcg cccacggtac gcatttgcag ataggttgcg ccctgagtaa tgatcatccc
     7441 tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga agttaccggt
     7501 gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa
     7561 cgctacacca attaccagcg gcggaacgaa gctaccaatg aagatgcccc agtcccacat
     7621 gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg gacggaagaa
     7681 caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag cggcatagac
     7741 catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc
     7801 ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac gaccgaggaa
     7861 acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac caatcagcag
     7921 aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca tttgacgact
     7981 cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt
     8041 tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag
     8101 gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc ctgcggtcag
     8161 tgacgaattc gccacagctg tcggcagcac ttcaccgata gcccacggtt ggcggccata
     8221 ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac cgtacagcgc
     8281 ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc agaaagagag
     8341 cgcgatgatt gccagcagca ggaagccaca cgccaccatg atacggaacg caaagtacag
     8401 cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt cagtcgcatc
     8461 agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct
     8521 attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt cgagcagaga
     8581 gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct ctttcaggcc
     8641 gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat
     8701 cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacaaagtaa aggcagcagg
     8761 cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct gcacgtcgcc
     8821 catttcgtag ccggattcat cacccagaac aataacagac agaacagcag ccataccgaa
     8881 gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt tcagcatata
     8941 ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg ctacagtgtg
     9001 aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca ccatctccat
     9061 acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc cgttcgcaac
     9121 cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga cacacatatg
     9181 ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg tggattcgag
     9241 gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc ctacatagtg
     9301 ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg tagccacacc
     9361 cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt tataaatctg
     9421 tttgccggag aggacgtaga ccgtttccat aatggccagg aggaacgcca taccgagcgt
     9481 cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc
     9541 gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga gtgaaccccg
     9601 ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc atcataccat
     9661 gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat
     9721 tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt tgttgaagca
     9781 aatttgcatt tttcgacggt gatcaattta tagctaaatt gccgcctttc agccaatttg
     9841 atcgagaaca atttatctct ttttgatgcc catttccaag acttatacat tgataaatat
     9901 caatttctac aaattatgtt aatcatgttt caacaaggta actttagtga tatttatgtt
     9961 taaaatgtgt gaattataag actgaaatta gtttcggttg gcaattaaag ataaaaagag
    10021 acgaagcaaa aacagcacgg ttgaccttaa ttcttttttt ggtcactggc taatacgacg
    10081 cagagtttca ggattattaa taaaggcata aatctccccc ccgccgatat taacatttaa
    10141 ttaacttata attatattat aattaactca cccgtggtta ttattttgtt aaaaataaag
    10201 ccacatatac gtagcttgag agtgaagata aacacaaagt tacctgatgc gctacgcttg
    10261 ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag accgaataaa
    10321 gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg gccgcgcata
    10381 tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg tgactggccg
    10441 ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat gtgttcatcc
    10501 atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc atcacaggtt
    10561 gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc agctttctta
    10621 agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact ttccggcacg
    10681 ttgaatccgg ctttgttgag cttcatcgca tcccatggga ttttgttgta acactgtact
    10741 ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt accggtataa
    10801 ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg gactggcatt
    10861 ttaattcccg aaggccgcct aggccttaaa agcagatctt ctttccccag taagcccacg
    10921 ccgcgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc aaattcacgt
    10981 agcccttcgc taaagacagc catgccgtta cgcccttcct gtaaggctgc atagttgagc
    11041 atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca
    11101 ctgtcgttca cggggcgcgt tagcgaacca aactgggtat ctgccagaac actatcggtg
    11161 ttaaaagatg tagggatcag gacgcgaagg cgatgatcgt cagcctggtt atcaaggttg
    11221 atatccacat caatgcgcct gttgttatga ctaagagtga caaccaactc gacatctact
    11281 ctgccagtgg attgtcttgc gctgcgttgt tgcagattac gcggcaccgc catttcatag
    11341 cggataacag ccctgctctg ccaggcctca tgaataatct cgcattgcgg tttcgcattc
    11401 gccgaagtaa ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca
    11461 gagctttctt caatttgcaa cacccggtca tagcgcacac cgctatcttt atctaccagt
    11521 tgtagcgttc catcctcatt gagcgcaatt tgccagaaag cattttccag tatcccttca
    11581 gcgtcacttt ttgccgcaat cacgttgcca ggctgattcg cttcgatata aagcgtgcga
    11641 tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa gggatcgtaa
    11701 ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg ggcatggcga
    11761 ataaaatacg gtacaggctg accgcgatcg tctcgtagat taaactggct ggcgcgcagc
    11821 cgcacagtgg tgttgatcac ttcttcccgc ggccagggca tcaggttaaa caggacgagt
    11881 ttgtcggcgt cgctctgcgg catgttgtcg gcgattttac gcatatagaa acgcagcaga
    11941 ttatccgcca tgtcttcagc cagttcgaag cgggcgacga tttcgcgatg aactttgtca
    12001 ctacagcagc agccgatgct gtcgtgggca tgatttttta agatctcttt ccacattttc
    12061 tccagcaagc cgtggtggta tccaaaaccc aacgtcctgg ccagcgttgc cagcggttcc
    12121 agcagattaa caatcttatt ttcaatacgt gcgtgggcaa ctttgatatc catgcgcgtg
    12181 gaaccgatgg tgcgatgcac gcgcatatat ttgccatcaa taaattcccc tttcagggtt
    12241 gccagattat ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca
    12301 aactttcgtt gaggataaat ttcgcgcagt ttctccatca cttcgaagat attttgctgc
    12361 aatggcatct gatcatgccc attcggcagc aaaatctctt tggttacaga cgctttttcc
    12421 agcacgtcaa aataactgtc gaggcgtttg cgtaatccgt tttcgtccgc aggtaagtac
    12481 ttaccgatgg cataacccaa cggcatcacc tgcgccgtca cttcgctacc gtcactgctt
    12541 tgccacaaaa actcggtttt atccgtaccg tggcgctcag aacatccacg ccagaacatg
    12601 gtgcaggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat gccaaaggaa
    12661 tcgggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac
    12721 atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtatactag
    12781 gggccgataa tcaacttgcc ggcttctacc agtttcttca cgcggtcttt gttttccggt
    12841 ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat
    12901 tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatgcgt
    12961 gactcttcgg tggtgaaata ccactcccga tcccagtgca tatgcggggt gatgtgaacg
    13021 cgagataccg ctttcatcgt cgtttccttt ttgtttatgg cattacgcca tcagtcagat
    13081 agttgccatg cttaaccgcg tgacgccgcc agatcaggag aattgcagtc gagattgcag
    13141 cccccaccaa tgccgcacca aaccagccaa ttgccgccat aacaccgccc gcgccattat
    13201 catgaagtaa aaagagcgag aaaatgccag caccgggtgt cgaaaggccg atattcatcg
    13261 caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg
    13321 gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg
    13381 tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag
    13441 cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa
    13501 cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttattcact ggccctccaa
    13561 ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca
    13621 gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttcc cccaccacaa
    13681 acagcatcag actgcccgct cccaatgtac cgagcaccgg gtagagataa aaagtcagga
    13741 agccattgaa tttactgcta agacgcaagt gatttttcat ccagcgtatc aagtaaccgg
    13801 ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg gcggcaagtc
    13861 cagccgcaaa gcctggcgct aacgccggtt tatctgccag cgaataggcg gtataggctg
    13921 ccagcaccgg taccatcaaa attccgagca gcccgccgcc cagtttgcgg tacatccaca
    13981 gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac agtaataccg
    14041 cgaccgccag caccgtcccc cccgcgacaa tcaacggtac ggcaaaagag atcccgctca
    14101 acagtgcctg tttcagctcc gttttgacgc ttttcaccgg ttgcgtatct tgttgtaagg
    14161 tacgcgtctc atcgccacgc tcgagggtaa gcgattgttg gatcaacgct tctgcatggc
    14221 gaatcggctc ggcaacaggc acagaaagcg caggaatgcc gttaaaacgc tcactctcct
    14281 tgatggcgac ttcagccgca aaaatacagg cagttgcact attgagttga tccgccgtta
    14341 aacgcccttc aataccgtta gcaccttgct tttcaacgta gacattcacg ccgagtttgc
    14401 gtccggcttt ttccagatac tccgcagcca tgtaggtgtg agcaataccc gccgggcagg
    14461 ccgttacgca aacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct
    14521 tgtcatccag cgccgagagc aattcatcag gcgtcgtcgc cgactgtata cgcgcccgaa
    14581 tctcatcatc cgccaggcgc gtggtcagcg ctgtcagcag ttgcatgtgc gtagtacccg
    14641 cttcattgag gggaatcgcc agcaacacca ctaaatcaac cgcttccggg ccatccacgc
    14701 cttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag
    14761 cagttttgcc atgcggcaca gccaaccctt cacttaaggc ggtcgggcca aggctttcac
    14821 ggcgatacac ttctttcaga aattgctcag tactggaaat tttccccaga gcagcaagac
    14881 gttgagtcaa cgcgtgaatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg
    14941 catcgcggtg ggtcagagtc gtcaggttca tcatgatcct cacttgatcg tttttatagt
    15001 cgcgccagtg tgcccgacaa aacaatacca ttgcgtaatc aatttcactt gattgaaatt
    15061 atttgcattt tatttgtatt gtttacagga tgaatttatc gggcataatg gctaaattcg
    15121 tattaatgag atgctaaata atgggacaca aacccttata ccgacagatt gctgatcgca
    15181 ttcgtgagca aattgcgcgt ggcgagctta aacctggcga tgcgttacct accgaatcgg
    15241 cattacaaac agagtttggc gtcagccggg ttacggtgcg tcaggctttg cgccagttag
    15301 tcgagcaaca gatcctcgaa agcattcagg gcagcgggac ttacgtcaaa gaagagcggg
    15361 tcaattacga tatttttcag ttaaccagtt ttgatgaaaa actgtcggac cgtcacgtcg
    15421 atacacacag tgaagttctg atatttgaag tgattccggc tgacgatttt cttcagcaac
    15481 agctacaaat caccgtgcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc tatcgcaagc
    15541 aaaagccaat ggcgctggaa gaaacctgga tgccgcttgc gttgttcccg gatctcacct
    15601 ggcaggtcat ggaaaattcg aaatatcact ttattgaaga agtgaagaag atggttatcg
    15661 atcgtagcga acaggaaatc attcctctga tgccaaccga agagatgagc cgtctgctta
    15721 atatcagcca gacaaagccg attcttgaaa aagtatcgcg tggatattta gttgatggtc
    15781 gggtgtttga atatagccgc aacgctttta ataccgatga ctataaattt accctgatag
    15841 cgcagcgaaa atcatcgcga taagcacaaa aaggccatca tacgatggcc aaccatgtcg
    15901 aaaccgacag cccttcgcca ttcctgtaga gggaagcggc gagggctatt tcttattaca
    15961 gatatttatt tcagaacagt tttcagtgct tcaccgatat ccgccaggct gcgaacggtt
    16021 ttcacgcctg cggcttccag agcagcgaat ttctcatccg cagtcccttt cccaccggca
    16081 atgatcgcac ccgcgtggcc catacgtttg cctttcggcg cagtcacacc agcgatgtaa
    16141 cccacaactg gcttggtaac atgctctttg atgtacgctg ccgcttcttc ttcagcgcta
    16201 ccgccgatct caccgatcat cacgatcgct tcggtctgcg gatctttttc gaacatttcg
    16261 agaatgtcga taaagttaga gccagggatc gggtcaccgc cgataccgac acaggtcgac
    16321 tgaccgaaac cgtaatccgt ggtctgttta accgcttcat aggtcagtgt accggaacgg
    16381 gaaacgatac ccactttacc cggtttgtga atgtgacccg gctggatacc aattttgcat
    16441 tcacccgggg tgataacgcc tgggcagttc gggccgatca tacgaacgcc tgcttcgtcc
    16501 agtttcactt tcacggtcag catatccagc gtcgggatgc cttcagtgat ggtgataatc
    16561 agtttgatgc ctgcatcgat agcttccaga atggagtctt tgcagaacgg tgctggtacg
    16621 taaataacag acgcggaagc gccagtagca gcaacagctt cacgcacggt gttgaacacc
    16681 ggcaggccga ggtgggtagt gccgccttta cctggagtta cgccaccaac cattttagtg
    16741 ccgtatgcaa tggcctgttc tgagtggaaa gtcccctggc taccggtaaa gccctggcag
    16801 ataaccttgg tgtttttatc aattaaaatg gacattattt cccctccact gcggcaacaa
    16861 cctgctgagc tgcatccgtc agaccttttg ctgcaataat attcaggccg ctgtcagcca
    16921 gtttcttcgc gccgagttcg gcgttgttac cttccagacg taccacgacc ggtacgttaa
    16981 cacccacttc cgctaccgcg ccgatgatac cgtcagcgat caggtcgcaa cgaacgatac
    17041 cgccgaagat gttaaccaga acggctttca ctttgtcgtc agagaggatg attttgaacg
    17101 cttcggttac acgttctttg gttgcgccgc cgccaacgtc caggaagtta gccggttcgc
    17161 cgccgtgcag tttaactatg tccatcgtac ccatcgccag acctgcgccg ttaaccatac
    17221 aaccgatgtt accgtccagc gcaacgtagt tcagttccca ctgtgcagcc tgtgcttcac
    17281 gcggatcttc ctgcgactgg tcacgcattt cgcgcagatc aggctggcgg aacagggcgt
    17341 tgccatcagc gcccagtttg ccgtcgaggc aaatcagatc gccctgtttg gtgatgacca
    17401 gcgggttgat ttcgatcagc gccaggtcgc gctccaggaa aatggtcgcc aggcccatga
    17461 agattttggt gaactgctga accagtttac cttccagacc cagtttgaac gccagctcgc
    17521 gtccctgata cggcatcggg ccagtcagcg gatcaagcgc aactttatgg atcaggtgcg
    17581 gagtttcttc cgccactttt tcgatttcca cgccgccttc ggtggaggcc ataaagacca
    17641 cgcgacggga actacggtct acaacggcac cgagatacag ctctttagcg atatcggtcg
    17701 ccgcttcaac cagaatctgg ttaaccggtt ggccattggc atctgtttga tacgttacca
    17761 gacgcttgcc cagccagttt tctgcaaaag cacggatgtc ttctttgctg tttacaactt
    17821 tcacaccgcc cgctttaccg cggccaccag cgtgaacctg acatttcact acccacggac
    17881 cggcaccgat ttttgaagcg gcttcttctg cttcgcgcgg agtagtacag gcataaccca
    17941 ccggtgctgg taagccatag cgggcaaaca gttgttttgc ctgatattca tgtaagttca
    18001 tgtgttctgt ccatccttca gtaatcgtta tcttttaaac cgtagaccga caccctatcc
    18061 acgtcggtct acagtgcagg tgaagcttaa actactacac gtccagcagc agacgcgtcg
    18121 gctcttccag taactctttg atcgttacca ggaagcccac ggattcgcga ccatcgatca
    18181 gacggtgatc gtaggacagc gccaggtaca tcatcggcag gatctcaacc tgaccattca
    18241 ccgccatcgg acgatctttg atagcgtgca tacccagaat tgcgctctgc ggcgggttga
    18301 tgatcggcgt agacatcagg gaaccgaaca caccaccgtt ggtgatggtg aagttaccac
    18361 cggtcagatc ttcaaccgtc agcttgccat cacggccttt gactgccagc tctttgattt
    18421 tcttctcgat gtctgccatg ccgagggtat cgacatcacg cagaaccggc gtcaccaggc
    18481 cgcgcggcgt agaaaccgcc atgctgacgt cgaaatagtt gtggtaaacc acgtcatcgc
    18541 cgtcgataga cgcgttcact tccgggtaac gtttcagggc ttcaaccacc gctttcacgt
    18601 agaaggacat aaagcccaga cggatgccgt ggcgtttttc aaacgcttca ccgtactgct
    18661 tacgcagatc cataatcggc ttcatgttga cttcgttgaa cgtggtcagc atggcggtgg
    18721 agtttttcgc ttccagcaga cgctctgcca cacgcttacg caggcgagtc atcgggacac
    18781 gtttttcact acgtgcagcc agagccggtt gcgccgccgg agcagccgct gccggagcag
    18841 actctttcgc cggggctttc gccagatgtt tttccacatc ttcacgagtc agacgaccac
    18901 ccacaccggt gcctttaatg gcgctggcgt cgagattgtg ttcagccagc aggcgacgga
    18961 tcgccgggct taacgcatcg ttgttttgct cttccagaga cgcctgctgg cgttgcgccg
    19021 gagtggacgc tttctcttca gatttggcgc tggtttcttt accggcgctg ttgccttcac
    19081 gcaggcgacc aaggatctga cgagacgtta ccgttgtacc ttcatcttcc agaaccgcat
    19141 ccagaatgcc gtctgctgat gccggtactt ccagtaccac tttgtcagtt tcgatttcta
    19201 ccagcacttc atcacgtacg actgcgtcgc cgggtttttt atgccaggtt gcgacggtgg
    19261 catcggctac ggattcaggc aggtcaggga ccagaatatc tacgctactc attgtgtatc
    19321 ctttatttat tcgacgttca gcgcgtcatt aaccagatct tgttgctgtt tctggtgaac
    19381 ggacatatac cctaccgccg gagaggcgga ggccgggcgg cctgcataac gcagagaagc
    19441 cccaaacgga atcacttcac ggaaatgatg ctggctgcag taccatgcgc cctggttgag
    19501 cggctcttcc tggcaccaga caaaatcctt gacgtgagca aactgctgca acacttcctg
    19561 catcgcttta tgcgggaacg ggtagagttg ctcgatacgc acaatggcga catcgtgttg
    19621 attgttctta cgacgctgtt ccagcaggtc gtaataaacc ttaccagaac acatcactac
    19681 gcgcttcacg cccttcggat caagctcgtc gatttcaccg atggctggca ggaaggtgcc
    19741 gttcgccagt tcttcgaggc tggaaaccgc cagcggatga cgcagcaggg atttcggcga
    19801 catcacgacc agcggacgac gcatcccgcg cagcgcctga cgacgcagca tgtggtaaac
    19861 ctgtgccggg gtagacggta cgcaaacctg catgttttgc tcagcacaaa gttgcagata
    19921 acgttccaga cgcgcggagg agtgctccgg cccctgccct tcgtaaccgt gcggcagcaa
    19981 catcaccaga ccacacatcc ggccccattt ctgctcgcca gaggagatga actggtcgat
    20041 aaccacctgc gcaccgttgg cgaagtcacc gaactgcgct tcccagatgg tcagggtgcg
    20101 tggttctgcg gtggcataac catattcaaa cgccagcacg gcttcttctg acagtacgga
    20161 gtcccagaca cggaacgcgc cctgaccgtt atggatgtgt tgcagcggcg tgtaagtgga
    20221 accgttagac tggttgtgga tcaccgcgtg gcggtggaag aaggtaccgc gaccggagtc
    20281 ttcacccgac aggcgaaccg gaatgccttc atcaaccagc gtggcgtaag cgaggttttc
    20341 cgcaccgccc cagtcgaaca gtttctcacc ggctgccatc gcctggcgat cgccataaat
    20401 cttggcaacg cgagactgca tttcaactgc ttctggtacc gtactgatac gtttagccag
    20461 ttcctgcagg cgcttcatct caactttgtt cgggtactct tcgtcccatt cgtggttgag
    20521 gtacggcgac caggtgaaag agtgcatgtt catcggacgc cactctgcca ctacgcaatc
    20581 gccagcatcc agcgcatcgc ggtacaggtt aaccatctcg gtggcatctt ccagcgtcgc
    20641 cactttttcc tgctccagct tgtcagcgta gattttgcgc ggtgtcggat gttttttgat
    20701 tttctgatac atcagtggct gggttgcgct cggctcgtcg gcttcgttgt ggccgtgacg
    20761 gcggtagcat accaggtcga tgaagacatc acgtttaaag gtgttacgga aatcgagcgc
    20821 cagacgggtc acaaaggcaa cggcttccgg atcatccgcg ttaacgtgga aaatcggtgc
    20881 ctgaaccatc ttaccgatat cagtacagta cggtgtagaa cgggcatcca gcgggttaga
    20941 ggtggtgaaa ccaacctggt tgttgataac gatacgtacc gtaccgccaa cttcataacc
    21001 acgcgctttc gacatgttca gagtttcctg aaccacgccc tgcccggtca ctgcggcgtc
    21061 accgtggatg gtgattggca gcactttgtt gctgttcggc tcatcaagtc tgtccagacg
    21121 ggcacgaaca gagccgataa cgaccgggct tacaatctca aggtgagacg ggttaaacgc
    21181 cagcgccagg tgcaccaggc cgccatcggt ctggaagtca gacgagaagc ccatgtggta
    21241 tttcacgtcg cctgtgccga ggtgttcttt atgtttaccg gcgaactcgt cgaacaagtc
    21301 ttgcggtttt ttacccagca cgttcaccag cacgttcaga cgaccacggt gcgccatccc
    21361 gagaaccact tcgcgggtgc cgctgttgcc agcgtggcgg atcatctctt taagcatcgg
    21421 gattaacgcg tcaccgcctt ccagcgagaa gcgttttgcg ccagggaatt ttgcgccgag
    21481 gtaacgctca aggccttcag cggcggtcag ttcgcttaag aagcgttttt tctcttcgct
    21541 attgaaagtc gcgcgaccag actcgatacg ctgttggatc cagcgttttt cttcggtgct
    21601 ggtgatgtgc atatactcgg caccaatcgg gccgcagtag gtttgcttga gggcggaaat
    21661 aagctcgccg agtttcatgg tttctttgcc gctggcaaat gaaccgacgt tgaaggtttc
    21721 ctggaagtct gcttcggtca gatcgtggaa agacggatcc agatcggcca ctttatcttg
    21781 ctgccacagt cccagcggat ctagattcgc atgctggtga ccacggaagc ggtatgcgtt
    21841 aatgagctgc aggactttaa cctgcttcac attggtgtca gggtcggaga tcgttgaaga
    21901 gtaacgtgaa gcgtctttcg ccaggcggcg gaaatattca cgcgtttgag agtggaattg
    21961 atccggtttg actcccgtac caggtaactg ctggaacgtc gaacgccagt tagcgtcaac
    22021 cgagtcagga tcggttaaga agtcttcata gagctgttct atccagctct ggtttgcgcc
    22081 agagaggtaa gaagagtcca accaggcttt caaagcgctg ttctgcatcg tgatccctta
    22141 agcatctttt ttatgcttac ttcgccgtgg atactaccac gcacagtgat gtgcgtgccg
    22201 gggttcactt gcgagctctt gtctacgtag tggctcgcga aggaaccttt aaaaactgtc
    22261 ttagtgtcag gggcagtttt taaagatttc ctgcatttct ttgttacgcc tgatgcgctg
    22321 cgcttatcag gcctgcgggt aatgacttgt aggccggata aggcgttcac gccgcatccg
    22381 gcactggttg cctgatgcaa cgcttgcgcg tcttatcagg cctacagttt acgcattacg
    22441 ttgcaacaac atcgacttga tatggccgat ggcgcgcgtc gggttcagcc ccttcggaca
    22501 tacactgacg cagttcatga tgctgtgaca gcggaatacg ctgaatgcat cgctcaaacc
    22561 gtcgaggcgg ctgtcagtct cggtatcacg gctatcgatc aggaaacgat atgccgctaa
    22621 caagcctgcc gggccgataa acttatcggg attccaccag aaagacggac aagaggttga
    22681 acaacatgcg cagagaatac attcatacag cccgtcgagt ttttcgcgct gctctggcat
    22741 ctgtaaatgc tcgcgagctg gcggattttg tccattattc aacaggtaag gcttaatttt
    22801 ctcatattgc gcatagaatt gtcccatgtc taccaccaaa tcgcggatca ccggtaaacc
    22861 tggcagcggg cgaatcacaa tcttcttgcc cggctggttg agtgccgaaa tcggggtaat
    22921 acaggccaga ccattcttgc cgttcatgtt cagaccgtcg gaaccgcaca caccttcacg
    22981 gcaggagcgg cggaacgaca ggctgggatc tttctctttc agctggataa gcgcatccag
    23041 cagcatcatg tcgcgacctt cttccgcttc cagggtgtaa tcctgcatac gcggagcatc
    23101 atcaacatcc gggttatagc gataaattga aaactcgagt ctcattttcc tgtctccgca
    23161 ttagtaagta cgaatcttcg gcgggaatgc cgggcgcagt ttcggttcca tgttgacgct
    23221 tcggcgcgtc atggattccg actcaggcag atacagggag tggcacagcc agttttcatc
    23281 atcacgatcc gggaagtcga agcggctatg tgcgccacgg ctttcggtac ggaagttggc
    23341 agaaacagcc gttgcatacg ccgtttccat caggttatcc agttccaggc actcaacgcg
    23401 ctgggtgttg aactcgctgg aagtgtcatc cagacgggca tttttcagac gctcgcggat
    23461 cactttcaac tgctcaagcc ctttcgccat cgcatcacct tcacggaaga ccgagaagtt
    23521 atgctgcata cattcttgca gcgctttacg gatcgccacc ggatcttcac cgttacgatt
    23581 attgttccac cggttcaggc gatccagaga cgcttcaaca tcagactcgc tggcatcgtg
    23641 cagtgcgccc tgctcggcga tagactcttg cagatgcaga cctgccgcgc gaccaaagac
    23701 caccaggtcc agcagcgagt tgccgcccag acggttagcg ccgtgtaccg atacacaagc
    23761 gatttcacca acggcaaaca gtcccggaac aaccacatct tcgcctttct cattcacagt
    23821 cagcgcctga ccggtaactt tggtcggaat accgcccatc atgtagtgac aggttgggat
    23881 aaccggaatc ggctctttca ccggatcgac gtgagcgaag gtacgggaaa gctccaggat
    23941 acccggcaga cgggattcga gaacttcttt acccaggtga tcgagtttca gtttcgcgtg
    24001 tggcccccac ggaccatcac agccgcgacc ttcacggatt tcaatcatga tggaacgcgc
    24061 caccacatca cggcccgcca ggtctttggc gttcggcgca taacgctcca taaagcgttc
    24121 gccatgtttg ttcagcagat aaccgccttc accacggcaa ccttcggtga ccagtacgcc
    24181 cgcaccggca atgccggtcg ggtggaattg ccacatttcc atatcctgca ccggtacgcc
    24241 tgcgcggata gccatgccga caccgtcgcc agtgttaatg tgggcgttag tggtggactg
    24301 ataaatacgc cctgctccgc cagtcgccag cacggtagcg cgggctttga aataaaccac
    24361 ttcaccggtt tcgatgcaca gtgcggtaca acccaccacc gcgccatcct ggtttttcac
    24421 cagatccagc gcataccact cggagaaaat ggtggtgtgg tttttcaggt tctgctgata
    24481 aagcgtgtgc aacagtgcgt gaccggtacg gtcagcagcc gccgcagtgc gtgccgcctg
    24541 ctcgccgccg aagtttttcg actgaccgcc aaacggacgt tgatagatac gaccatcatc
    24601 gagacgcgag aacggcaggc ccatgtgttc gagttccaga atcgcttccg gcccggtttt
    24661 acacatatat tcaatcgcgt cctggtcacc gatatagtcc gaccctttta cggtgtcgta
    24721 catatgccat tcccagttat cttcatgggt attacccagc gcaacggtaa tgccgccttg
    24781 cgcagaaacg gtatgggaac gggtcgggaa gactttagag agcagggcac aggtctggcc
    24841 gctctgggaa atttgcagcg ccgcgcgcat acctgcgcca ccggcaccaa tcacaactgc
    24901 atcaaattct ctgactggca atttcatcac acaccccaca ccacaacgaa tccataaatc
    24961 acgtaaacca ccagtgcaac gacaatcacc agttgcagca tcaggcgcaa ggccagcggt
    25021 ttaacgtagt cggtcaacac ctgccacatg ccgatccagg catgaatcaa gatagaaaac
    25081 agcgccagca gggtgaacac tttggtgaac gcagaggcga agaaaccgat ccagacttca
    25141 tatgtcagtt cgccactggt ggcgaaaaaa ccgaccatat aaatgatgta gagcgtcagg
    25201 acgatagcgg tagcgcgaac gaggatgaaa tcatgtacgc cattgcgtcc taatgcggag
    25261 gcgttgctta ccatacgagg actcctgcga gaagtgaaag cacgacagta ataacaaagg
    25321 agattttggc ggagcgttta cccgcttcga atgtttcttc cagatagcca aaatccatca
    25381 tcatgtggcg aatacctacg acgacgtgat acgccagagc ggtaaggatg ccccacatga
    25441 taaatttgac gaagaagctg cccataatcg cggaagcttg ctcgaaacct tcaggggaag
    25501 agaggctggt acccagaagc cactgcagga tgcccactgc aacaaaggtg atcacaccgg
    25561 aaacgcgatg gagaatggac gctatcgccg tgatggggaa ccggatggtc tgtaggtcca
    25621 gattaacagg tctttgtttt ttcacatttc ttatcatgaa taacgcccac atgctgttct
    25681 tattattccc tggggactac gggcacagag gttaactttc tgttacctgg agacgtcggg
    25741 atttccttcc tccggtctgc ttgcgggtca gacagcgtcc tttccataac tgcgcgtcat
    25801 gcaaaacact gcttccagat gcgaaaacga cacgttacaa cgctgggtgg ctcgggattg
    25861 cagggtgttc cggagacctg gcggcagtat aggctgttca caaaatcatt acaattaacc
    25921 tacatatagt ttgtcgggtt ttatcctgaa cagtgatcca ggtcacgata acaacattta
    25981 tttaattttt aatcatctaa tttgacaatc attcaacaaa gttgttacaa acattaccag
    26041 gaaaagcata taatgcgtaa aagttatgaa gtcggtattt cacctaagat taacttatgt
    26101 aacagtgtgg aagtattgac caattcattc gggacagtta ttagtggtag acaagtttaa
    26161 taattcggat tgctaagtac ttgattcgcc atttattcgt catcaatgga ttctttacct
    26221 gcaagcgccc agagctctgt acccaggttt tcccctcttt cacagagcgg cgagccaaat
    26281 aacaaacggg taaagccagg ttgatgtgcg aaggcaaatt taagttccgg cagtcttacg
    26341 caataaggcg ctaaggagac cttaaatggc tgatacaaaa gcaaaactca acctcaacgg
    26401 ggacacagct gttgaactgg atgtgctgaa aggcacgctg ggtcaagatg ttattgatat
    26461 ccgtactctc ggttcaaaag gtgtgttcac ctttgaccca ggcttcactt caaccgcatc
    26521 ctgcgaatct aaaattactt ttattgatgg tgatgaaggt attttgctgc accgcggttt
    26581 cccgatcgat cagctggcga ccgattctaa ctacctagaa gtttgttaca tcctgctgaa
    26641 tggtgaaaaa ccgactcagg aacagtatga cgaatttaaa actacggtga cccgtcatac
    26701 catgatccac gagcagatta cccgtctgtt ccacgctttc cgtcgcgact cacatccaat
    26761 ggcagtcatg tgtggtatta ccggcgcgct ggcggcgttc tatcacgact cgctggatgt
    26821 taacaatcct cgtcatcgtg aaattgccgc gttccgcctg ctgtcgaaaa tgccgaccat
    26881 ggccgcgatg tgttacaagt attccattgg tcagccattt gtttatccgc gcaacgatct
    26941 ctcctatgcc ggtaacttcc tgaatatgat gttctccacg ccgtgcgaac cgtatgaagt
    27001 taatccgatt ctggaacgtg ctatggaccg tattctgatc ctgcacgctg accatgaaca
    27061 gaacgcctct acctccaccg tgcgtaccgc tggctcttcg ggtgcgaacc cgtttgcctg
    27121 tatcgcagca ggtattgctt cactgtgggg acctgcgcac ggtggtgcta acgaagcggc
    27181 gctgaaaatg ctggaagaaa ttagctccgt taaacacatt ccggaatttg ttcgtcgtgc
    27241 gaaagataaa aatgattctt tccgcctgat gggcttcggt caccgcgtgt acaaaaatta
    27301 cgacccgcgc gccaccgtaa tgcgtgaaac ctgccatgaa gttctgaaag agctgggcac
    27361 caaagatgac ctgctggaag tggctatgga gctggaaaac atcgcgctga acgacccgta
    27421 ctttatcgag aagaaactgt acccgaacgt cgatttctac tctggtatca tcctgaaagc
    27481 gatgggtatt ccgtcttcca tgttcaccgt cattttcgca atggcacgta ccgttggctg
    27541 gatcgcccac tggagcgaaa tgcacagtta cggtatgaag attgcccgtc cgcgtcagct
    27601 gtatacagga tatgaaaaac gcgactttaa aagcgatatc aagcgttaat ggttgattgc
    27661 taagttgtaa atattttaac ccgccgttca aatggcgggt taatttttat atgcctaaac
    27721 acaaaaaatt gtaaaaataa aatccactat cggacattca tatattctaa aaaaaaatac
    27781 atcggtataa attataaaca aatagatgat ttttaatcat ctatcttcct ctcagttaca
    27841 tgaataaacc accaaatata gcgcacccct ataattccca atactttatt catcaaacat
    27901 ttaattcata ccatcaatta attactttta catttaataa aattaataat atttttaaga
    27961 aaaactaaaa acattcgaca atcaaataag atattcttgc accgatatac ttttttcttt
    28021 tctgttaaag gactcagtta tgtttaaagg acaaaaaaca ttggccgcac tggccgtatc
    28081 tctgctgttc acagcacctg tttatgcggc tgatgaagga tccggtgaaa ttcactttaa
    28141 aggtgaagtt attgaagcac cgtgtgaaat acatcaggat gatattgata aagaggttga
    28201 actcggtcag gtgaccacca gccacattaa tcagtcacat cacagcgatg ccgttgctgt
    28261 cgacctgctc ttagtcaact gtgatctgga aaactccagc aacggttccg gtggcaagat
    28321 ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc accggcgcag atccgattct
    28381 caacaacacc agcacgggtg aagccaccgg cgtcggcgta cgtttaatga ataaagatca
    28441 aagcaatatc gttttaggca ccgctactcc agatatcgac ctggctccga cctccagcga
    28501 acaaacgctg aatttctttg cctggatgga acaaattgat caggctacac ctgtaacgcc
    28561 aggcgcagtt acggcgaatg caacctacgt gctggattat aaataagtat tactttggcg
    28621 gcgctatcgc cgcctttcat aaaatattta tcaggtatgg acaccgtgaa tatttatcga
    28681 ctctcttttg tatcctgcct ggtcgtggcg atgccttgcg cattggcggt cgaattcaat
    28741 ctgaatgttc tcgataaatc aatgcgcgac cgcattgata tttcattatt aaaggaaaaa
    28801 ggagtcattg ctcccggtga gtattttgtt agcgttgcgg taaataataa ccaaatcagt
    28861 aacgagcaaa agattaactg gcacaaaaat gacgataaaa ccattccgtg catcaatgat
    28921 ttactggtcg ataaatttgg cttaaaacct gaagtccgtc agtcgttacc attgataaat
    28981 cagtgcgtcg attttagctc ccgacctgaa atgctcttca atttcgatca agccaatcag
    29041 caactgaata ttagtattcc gcaagcctgg ctggcgtggc actcagaaaa ctggacccca
    29101 ccctccacat ggaaagaagg tgtcgccggt gtcctgatgg attacaactt gtttgccagc
    29161 agctaccgcc cacaggatgg cagcagcagc actaatctta acgcctatgg aacagccgga
    29221 atcaacacgg gggcatggcg cttacgcagt gattatcagt tgaatcatac tgatagcgat
    29281 gataaccatg aacagtcagg cgaaatatca cgtacctatc tttttcgccc gttaccgcaa
    29341 ttaggttcaa aattaaccct cggcgaaacg gattttagtc ccaatatttt cgacggtttt
    29401 tcttataccg gcgcggcact ggcaagtgac gatcgaatgt tgccatggga actacgcggc
    29461 tacgccccac aaattagcgg tattgcacag accaatgcca cggtgacgat cagtcaatca
    29521 ggccgcgtca tttaccagaa aaaagtccca ccaggcccat ttatcattga cgaccttaat
    29581 cagtctgttc agggcacact ggatgtcaaa gtgacggaag aagatggtcg ggtgaacaat
    29641 ttccaggttt cagcagcatc gacgcccttc ctgactcgtc agggacaggt tcgctataaa
    29701 ctggccgcgg gtcagccacg gccctccatg tcacatcaaa ctgaaaatga aacctttttt
    29761 agcaatgaag tttcctgggg gatgctgtca aacacctcgc tgtacagcgg cctgctgctt
    29821 tctggtgatg actaccattc tgccgcaatg ggtattgggc aaaatatgct gtggcttggt
    29881 gcgctgtcgt ttgatgtcac gtgggccagt agccattttg atactcagca ggacgagcgg
    29941 ggcttaagct accgttttaa ttacagcaaa caagtggatg ccactaacag cacgatttcc
    30001 ctcgccgctt atcgtttctc cgatcgtcat tttcacagct acgccaacta tctggatcac
    30061 aaatacaacg acagcgatgc gcaggacgaa aaacagacga tcagcttatc tgtgggccaa
    30121 ccgattaccc cactaaacct caatctttac gccaacctgc tacatcaaac ctggtggaat
    30181 gcagacgcct ccacgaccgc caacatcacg gcaggtttta atgttgatat tggtgactgg
    30241 agagatatct ctatttctac gtcattcaat acaacccatt acgaagataa agatcgcgac
    30301 aaccagattt acctgtcgat ttcgctcccc ttcggtaacg gtggtcgggt tggctatgac
    30361 atgcaaaaca gtagccacag caccacacac cgcatgtcgt ggaacgatac gctggatgaa
    30421 cgtaatagct ggggcatgtc tgccggactg caatccgacc gtcctgacaa tggagcccag
    30481 gtgagcggta actatcagca cctgagttca gcgggtgagt gggatatttc tggtacctat
    30541 gccgccaatg attacagttc cgtcagcagc agctggagcg gttctttcac cgcaacccaa
    30601 tatggtgcag cgtttcatcg ccgcagctcc accaatgaac cacgcctgat ggtcagcacc
    30661 gatggcgtgg cagatattcc ggttcagggc aatctcgact acaccaacca ttttggcatt
    30721 gcggtggtgc cgttgatttc cagttaccag ccttccaccg tggcggtgaa catgaatgac
    30781 ttacccgacg gcgtaacagt tgcagaaaac gttatcaaag aaacgtggat tgaaggcgcg
    30841 ataggttaca aatcactggc ttcccgttcc ggtaaagacg ttaacgtcat cattcgcaac
    30901 gccagcggtc agtttcctcc cctcggagcg gatatccgcc aggatgacag cggcattagc
    30961 gtggggatgg ttggcgagga aggacatgcc tggttaagcg gagtcgctga aaatcaaaag
    31021 tttaccgtgg tctggggtga tagccagcat tgctcgctcc atcttcctga acatatggaa
    31081 gacaccgcaa atcgcctgat tttaccttgt cattaattaa ggaaatgaaa atgacattta
    31141 tgaaaggact accccttttg ttgttggttg ccagtctgtg cagccacgct gcactacaac
    31201 ccgatcgcac tcgtattgtg tttaacgcta atgataaagc taccagtctg cgggtggata
    31261 atcgtagcga taaactcccc tacctcgctt attcctggct cgaaaatgaa aaaggggaga
    31321 aaagcgatga tcttctggtc gccctgccgc cgatacaacg tctggaaccg aaagcaacaa
    31381 cacaggtacg gatagtaaag caagcctcga ccacaaaatt accgggcgat cgcgaaacgc
    31441 tatttttcta caatatgcgt gaaattcctc ccgccccgga aaaaaacagc gaccatgccg
    31501 ttctgcaagt cgccattcag agtcgtatca aggtgttctg gcgaccggct gcattacgca
    31561 agaaagcagg agaaaaggtc gaactgcagt tacaggtcag ccagcagggc aaccaactga
    31621 cactgaaaaa tcctaccgcg tattatctga ccattgccta cctgggacga aatgaaaaag
    31681 gcgttctccc cggcttcaaa accgtgatgg ttgcgccttt tagtacggtc aacacaaaca
    31741 ccggaaatta tagcggcagc cagttttatc tcggttatat ggatgattac ggcgcgttac
    31801 gcatgacgac gctcaactgc agcggacaat gccatttaca agccgtggag gcgaagaaat
    31861 gagtgctggc aagggagtgt tgctcgtcat ctgtctgtta tttctgtcgt tgaagtctgc
    31921 gcttgccctg aactgctact ttggttcatc cggtggctcg gtagaaaaat cagaagcgat
    31981 tcaacctttt gctgtaccag gcaatgccaa actcggcgat aagatctggg aatctgacga
    32041 tattaaaata agatctggga atctgacgat attaaaattc ccgtctattg tgacaacaat
    32101 accaacggca actttgaaag cgaacatgtc tacgcatggg taaaccccta cccgggggtg
    32161 caggatcgtt attatcaact cggagtaact tacaatggcg ttgattatga cgccaaccag
    32221 ggaaaaagcc gcatcgacac taaccagtgt atcgacagta agaacattga catttatacc
    32281 cctgatggga tggcagaaca aaatttgctc gggtgatccc gctaatattc acatgtcgcg
    32341 aacctttctc gctcgcatgc ggttatacgt caaaatacga gaaatgccgc cgcatgatta
    32401 tcaaagtacg cttagcgact atatcgtcgt gcaatttgac ggtgccggta gcgttaacga
    32461 agaccctact gcccaaaacc tgaaatatca tattactggt ctggaaaaca ttcgcgtgct
    32521 ggattgcagc gtcaattttt ccatttcacc ggaaacacag atgattgatt ttggtaaatt
    32581 taatttgctg gatattcgtc gccacacaat gtcgaaaaca ttcagtatta agacgacaaa
    32641 atcacaaaat gaccaatgca ccgacggatt taaagtcagt tcctcctttt ataccgaaga
    32701 aacgttggtt gaagaagata aagcattgtt gattggtaat ggtctgaagc tacgtttgct
    32761 ggatgaaaac gcctcgcctt ataccttcaa taaatatgct gaatacgccg atttcaccag
    32821 cgacatgttg gtctacgaaa aaacctatac agctgaactt tcgtccattg caggtacccc
    32881 catcgaggct gggcccttcg atacagtggt gctttttaaa attaactata actgatttgc
    32941 catgatgccc tttgcagccg gaaagggcat ctctttcaga aaattgctga ttttcccctt
    33001 ttccttgatt gctgttattt tttcctagcc cggctttctt tatgctgtat cgatacacca
    33061 aagagaaaat aatgagggag cgaaggatgc cagttttgca gtggggaatg ttatgtgtgc
    33121 tatcacttct cctttctatt ggtttcctcg cggtacatct cccggcggcg ctattacttg
    33181 ggccgatgat cgccgggatc atcttcagta tgcgcggaat tactctgcaa cttccccgct
    33241 ccgcttttct cgccgcgcag gccattcttg gctgcatgat tgcgcaaaac ctcactggtt
    33301 cgattctcac cacgctggcc gccaactggc cgatcgtgct tgctatttta ctggtgacgc
    33361 tgctttccag cgccatcgtg ggttggttat tggtgcgcta tagctcactg cctggaaata
    33421 ccggtgcctg gggttcttct ccaggcggcg cagcggcaat ggtcgctatg gcgcaagatt
    33481 acggcgcaga tattcgcctg gtggcgttta tgcaatatct gcgagtgctg ttcgtcgcgg
    33541 gggccgctgt tctggtcacc cgcatgatgc tgggtgataa cgctgaagcg gttaaccagc
    33601 aaatcgtctg gttcccgcca gtgagcatta atctcctgct tacgattttg ctcgcagtcg
    33661 ttgccggtac ggcgggatgc atgctgcgtc ttccttccgg cacgatgctc atcccgatgc
    33721 tggcgggcgc agtgctccag tctggtcagc tcatcaccat cgaactaccg gaatggctgc
    33781 tggcgatggc gtatatggca attggctggc ggattggtct tggtttcgat aaacaaatct
    33841 tactgcgggc actacgcccg ctaccgcaaa tcctgctgtc gatttttgct ctgctggcta
    33901 tttgtgcagg tatggcgtgg ggactgaccc ggtttatgca tattgatttt atgactgcct
    33961 acctcgccac cagtcccggc gggcttgata ccgtggcggt catcgccgca gggagcaatg
    34021 ccgatatggc gcttattatg gcgatgcaaa ccctgcgcct gttcagtatt ttgctgacgg
    34081 ggccagccat tgcgcggttt atttcaacct atgcgccgaa gcggtcggcc tagtgctggc
    34141 agcctggaca ccagtaaaac gggcgcgagg acaacgtggt tttctcaatg atgccgccac
    34201 agcgttcgca cagttcgcca tcacgatgaa aaaccttaaa gcgaaacagc gccccatgat
    34261 gcttattctc atccacctgc ccccgcgtag cgtaagaaaa ccgaggaatc tccagtaacg
    34321 cgtgtgcgag tgcatccagt tgcgccgcat tgagatcttt cgctttatga tttccagtca
    34381 acccaacctg ccagaggatc tccacccgca aataattgcc aagcccagcc agaaacgcct
    34441 gatcgagcag taatcctgca aactgacggt tacgaaagcg cggcgacaat aaccgttcct
    34501 ttaccacctc cggcgtcaga ttcggatcca gcacatcggg accaacgcgt tgtaaaaacg
    34561 gatgcgtggt cagttgttcc ggcctcaaca tctcaatatc cgaggcgcta taaagcagaa
    34621 tggttttgtc agccgtttgc agttttaccc gcaatactcg cgtggtctgc ggctcttcgc
    34681 cggtatcaac cacgcgccag acgccgtaaa gctgattatg gctgtagagc gttaaaccgt
    34741 tgggaaaatg agttaacaac gccttaccac gcgtttccac atgggtaacg tgttgaccga
    34801 taagttgtga ttgataagtt tttaactgcg ggaaggcaaa ccagacatca gttagtggtt
    34861 tgcctttgat cgccgcctcc aggttatccg ctgcacggcg gatctccggg ccttcaggca
    34921 tggtgatatc cttttttcat tctttattaa gcggggttat gctgcgacaa caatcccctt
    34981 tttggcaaat gtagctcgta atcggcgggc gaacgccaga gcgtgctcgc cgtcgccatg
    35041 caggcagacc gtttgcgccg cgaccgttgc ccattcgccg gtgatacttt tgactctgcc
    35101 gtgttgcacc atctccagcg tttgcgccaa cgcctgttct tcgttttcaa tcaacgcgcc
    35161 tggctgactt cgcggcacca gcgagccgtc agcctgataa ccgcgatcgg caaacacttc
    35221 ctcgcgcgtt gtcaggccat attgcttgcc tgcacgaatc agctcgcttc ctgccagccc
    35281 aacgagaacc aatgccggat cgcaagcgta taccgctctg gcgatggcgt ctgccagttg
    35341 tgcttctttc gccgcctggt tgtacaacat gccgtgcggt ttgacatgac gcattacgcc
    35401 gccttgcgca cgggcaatcg ttgccagcgc gccaatttga tacagcgtct gggcgtaaac
    35461 ggtttccggc ggcagctgca tggcgctgcg accaaaattt tccctgtcgg gaaaactcgg
    35521 gtgagcgcca atcgcgacac cattttttat tgcttcacgc acgcaagcct gcatggtttg
    35581 cgcatcgcct gcatgaaatc cacaagcaat attggcagag gaaaccagcg ttaatagctc
    35641 tgcgtcgctg gcgcagcctt cgcccagatc ggcgttcagg tcaattttca ttgtgcagcc
    35701 gccacgctaa ttgttcaaaa taacgttgct gatcttgccg cgctttcagt gcctcttcca
    35761 gtgaacactg gacaaaatga atcggctgac cgagcggaat ttgcgccaga tggtacatat
    35821 cagcctcaat gatacaggca atacgtggat aaccaccggt ggtctgtgcg tcgttcatca
    35881 acacaatcgg ctgcccgtta tgcggcacct gcaccacgct cggtaacaaa ccgtgagata
    35941 acagttcgcg atcggtgatg cgttttaaaa tttgcccctg taaacgatag cccattcggt
    36001 tactttgcga gctaagctgc cagggcgaac gccagaatgc atcctgcgag gcgcgatcga
    36061 actcatgata ttccggcccc ggcaaggcgc gaatgcggtt gccccacagc agctgtttaa
    36121 cgccctgcgc ttccataaaa tcacgcttcg atttgccaat cgggagtcag tcaccatcct
    36181 tcagtaaacg gccttccagc ccgccaatcc ccactttgag atcggtgctg catgacccca
    36241 ttaccggcgg aacatcaata ccacccgcga ccgccagata actgcgcatc ccgtgctgcg
    36301 ggcgtttaag cgttaaacgc tggcctgctt tcatcggcaa tcgccagccg gtccagacgg
    36361 cgttatcatc caaccgcgct tcgcaacctg cacccgtcag ggcaaaccac ccatcagttt
    36421 cgaactcaac tgttaactga ccgagcgtga tctccagcgc gggggcattg gcgtcattac
    36481 ccaccagcag gttagcaatg cgcagcgcag gcatatccag tgcgccacag tggctgatac
    36541 ccgactggca aaaaccgtga cgaccgccat cctgcacagt ggtgtacatg cccgcacgaa
    36601 taatcttcag catattccct ccttctgcgg tacaaatcgc acgctgtctc ccggacgtaa
    36661 taagatgggt tcgtcacgcg ccggatcaaa caggctgagt gaggtatgac caatcaactg
    36721 ccagccaccc ggcgttgcca gcggataaac accagtctgc ggcccgccga tcccgacaga
    36781 acctgccgga acgagcaagc gcggttcagc gcgccgtggc gtgtgtagtt gttccggcaa
    36841 actcccgaga tacgggaagc ccggttgaaa acctaaaaac cagaccacgt attccacgga
    36901 ggagtgcaat tcaacaacct gtttttcgct caacccgcaa tacgccgcga ccaccgccaa
    36961 atccggtccg cctgcaccac cgtaaaccac cggaatttca ataaagcgag actcaggttc
    37021 cagggcctcg ctctcctccc accagcgttg caaacgctct atggcatcca gcgccagcga
    37081 ctcaggatta cgcaaaatca ccgtgatatt gttcatgccg ggaatggctt caaccacatt
    37141 cggcatatcc accagacgct gcgccagtcg ccagatccgt ttctggctag ccagcgtcac
    37201 cggcggttcc agttccagca ctaccgccgt ttcacctatc agataacaac gcgctcgttg
    37261 cactttcgat ccctctgatt attcgttatg caggattagg aatatcaata aaggtcacat
    37321 caagatcggt attttcattc agccactcgc tcaatgcgcg aataccaccg cgttcagtgg
    37381 catggtgacc tgcagcataa aaatgcaatc cctgctcgcg ggctgaatga atggtctgtt
    37441 cagaaacttc gccagtaata aaagcatcca cgccaaaacg cgcggcgcta tcgataaaac
    37501 tttgcccgcc gcccgtgcac caggcgacgc gctggaccac ctcaggtccg gtatcgccac
    37561 accataacgg cttacgtccc agacgcgctt caatccagga ggctagttcc attcccggca
    37621 caggcatggt cagctctccc cacggcacca acggctcaat ttcgcccatg accgtgatcc
    37681 ccagtaacgc cgccagctgt gcgttattgc ccagctcagg atgtgcgtca agcggcaaat
    37741 gccagccata caggttgata tcattcgcca gcaacgtttt taaacggtta cgcttcattc
    37801 cgcgaatgac cggagactct cctttccaga agtagccatg atgcacaatg accgcatctg
    37861 cgcccagacg cactgcctca tcgagcaaag cctggctggc ggtgacaccg gtaacaattt
    37921 tttgcaccgt ctctttgcct tccacctgca aaccattcgg cgcgtaatca ctaatcgccg
    37981 cgctgttcag tttttcgtta atcagttgtt ccagttcggt gtttttcatc ttcgcgcctt
    38041 ccgtattctg atagctacac cgttaacata gcgttacatc gcctgttcgt cgatagccaa
    38101 tcttttgaca tattaagaaa caaacccgac gccaaaagac gcaaattgtt tatatcatca
    38161 cgcttcagct aatgtatatt tatgcaatca gacagcatat tgcataaata atcagatctg
    38221 tatcaaagtt ttaactttaa ggaaacacaa gcacgccgcg caactattac gtgacgttgc
    38281 cgtcaattgt ggaattatta tgaataaaca cgcatcacaa ccgcgcgcta tttactatgt
    38341 cgttgcgctg caaatctggg aatattttag cttttacggc atgcgtgccc tgctgattct
    38401 ctatctcacc aatcaactaa aatacaacga tactcacgcc tacgagttat ttagcgccta
    38461 ctgttcgctg gtgtatgtca cgccaatcct cggtggcttt ttggcggata aagttctcgg
    38521 caatcgcatg gcggtgatgc tgggggcgtt gttgatggcg atcggtcatg tggtgctggg
    38581 tgccagtgag atccatccgt cattcctcta tctgtccctg gcgattatcg tctgcggcta
    38641 tggcctgttt aaatctaacg taagctgtct gctcggcgag ctgtatgagc caaccgatcc
    38701 gcgtcgtgat ggcggtttct cgctgatgta tgcggcgggt aacgtgggat ctattatcgc
    38761 acctatcgcc tgtggttacg cccaggaaga gtacagctgg gcgatgggct ttggcctggc
    38821 ggcggttggc atgatcgcgg gtctggtcat tttcttatgt ggcaatcgtc atttcactca
    38881 tacccgcggc gttaacaaaa aagtactgcg tgcgacaaac tttctcctgc cgaactgggg
    38941 atggctgctg gttctgctgg tggcaacgcc tgcactgatt accgtgctgt tctggaaaga
    39001 gtggtcggta tacgccttaa ttgtcgcgac tattattggt ctgggtgtac tggcaaaaat
    39061 ttatcgcaaa gcagaaaacc agaaacagcg gaaggagctg gggctgattg tgacgctcac
    39121 cttcttcagt atgttgttct gggccttcgc acaacagggc ggtagctcga ttagccttta
    39181 tatcgaccgc ttcgttaacc gcgatatgtt tggttatacc gttccgaccg cgatgttcca
    39241 gtcgattaat gccttcgcag ttatgctgtg cggtgtgttc ctggcgtggg tggttaaaga
    39301 aagcgtcgca ggtaatcgta ccgtgcgcat ctggggaaaa tttgctcttg gccttggcct
    39361 gatgagcgcc ggattctgca ttctgacctt aagcgcccgc tggtccgcaa tgtatggtca
    39421 ctcttctctg ccactgatgg tgttaggcct cgcggtgatg ggctttgcgg aactgtttat
    39481 cgacccggtt gccatgtcgc aaattacgcg cattgaaatt ccgggcgtga ccggcgtatt
    39541 aaccggcatc tacatgctgc tttctggcgc gatcgcgaac tatctggcgg gcgtgattgc
    39601 cgatcagaca tcgcaggctt cgtttgatgc ttccggggcg atcaactact ccatcaatgc
    39661 atatattgaa gtatttgatc aaattacctg gggcgcactg gcgtgtgtag gactggtact
    39721 gatgatttgg ctgtatcagg cgctgaaatt cagaaaccgc gcgctggcgc tggagtcata
    39781 ataggccagc accgatgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacgtatttc
    39841 ctgcaattta ttgaatttgc acaaatttgt aggctggatt aggcgttcac gccgcatccg
    39901 gcatctgggc tcgattgcct gacgcgtctg ttatttcccc ttccgcgccg cctcatacgc
    39961 tgccaacgtt tgtactctcg cctctttgtg ctcgactatc ggttgcggat aatccagctt
    40021 cacacctgct ttctgcgccc acttccacgg ctcatgcacc gctttccctg gtacatcgcg
    40081 cagctccggt aaccatcgac gaataaactc gccctcacgg tcaaatttct cgccctgagt
    40141 tatcggattg aaaatacgaa aatacggcgc agcatcagta cccgtagaag ccgcccactg
    40201 ccagccaccg ttattagctg ccaaatcacc atcaatcagc tgcgacatga aatatcgctc
    40261 gccttcgcgc cagtcgatca acaaatcttt caccagaaaa ctggctgtaa tcatccgtag
    40321 ccggttatgc atccagccag tgctgttaag ctgacgcata gcggcatcaa taatcgggta
    40381 tcccgttttg cctttctgcc aggcctgtaa atgtgcggga ttgctttgcc actgcacacg
    40441 atccgtccag gcaataaatg gacgatgttt acacaacgag gggtaatacg tcatcagatg
    40501 acggtagaac tcgcgccaga tcagctcact aagccagaca ctaccggccc caccgtccag
    40561 cgcctgcggc tgttccgcca acaagcgatg caaacactgg cgaggcgata acccgcccgt
    40621 tgccaagctg gcggacaaac ggctggtgcc ttccactgcc ggaaaatctc gttgttgctc
    40681 atactctcca gcaccgttct cgcaaaactg gcgtaattgc gcaatcgccg ctttttcttc
    40741 caccgggaaa tgcgcagtat cgaaagactg acgaggataa ttcagcgtaa tggatggcgc
    40801 gggctttatc gatccgctac tacgaacttt tggtgcagcg acgcactccg gcatcccttc
    40861 ccgcagccgt ttcagccagg cattcttaaa aggcgtaaag actttgtaca tctcgtgatt
    40921 gccggtcatc accgcccccg gcggcaggat cacgctgtca tcaaatcctt cacacaccac
    40981 gttacgcagc gctctttcaa cgtccacatc ccgcgctcgc tcattcacct catactgata
    41041 gttataaaaa agatgagtaa cgctgttttc cgcgcacacc tgtttaacta tttcgacact
    41101 ggcgacaaag tcatccactt cacggaacaa taaaggaata cctttttccg ccagcgcgat
    41161 ttgtagccca ttcagttgag cattgataag ttcagcctga cgcggcgaca tgttatgcgt
    41221 ctcccactgg cgtggtgtag cgatatacag cgccagcacg cgtgcagacg aattgcggca
    41281 ggcggcagcc agtgcgagat tatcgtgcag acgtaaatcc tggcgaaacc aggccagatg
    41341 ggtagtcata aaactcctga atggcgcaag ttaatcacac gcggccaaag ttcgaaataa
    41401 cgctgccctg aaagccaggc gtcaggataa agcgccatat agtgtttgat acgcatcagc
    41461 ggcgcaagaa gtggttgctc gccacggcga taactgtcga taagcgcagc cagctgctgg
    41521 cgttctgtgg aatcaatatg cgggcgaaaa taaccctgaa cgtgcatcaa aacgttggtg
    41581 tgatcgcgcg ggttggcggg atgagaaagc aaaacaatta cgcgttggcg gtactggttg
    41641 taaaaatcat ccagattttg ccactgatga atatcagcca caaacgggcc aagtttgcgg
    41701 tactcgggct gagaatgggc cagaaagacg agtttatagc ggctgtgata atcgagaagc
    41761 gccccgcgcg ttaagccatg ttctttcagt cgatgtaact catgaagggc gaagatacgg
    41821 attagggtgc tgtcatcaaa tcgttgtaga ttcattactg ctcctgaacg gtatacagga
    41881 gtaagtgtag gcgttggtaa atgatgtttg tgaagttaat ccgctagcag ggcaagatta
    41941 cgaaagcgcg ctccccgcaa gcaactaatc aaggtagtta atcatggctg aacacgatat
    42001 tacgccagaa gtcaccattt ccaaaacaca gcgccgctat aaagtcggct atgtcagagc
    42061 ccgccaccag gaccgcaaaa ccggtatagc ccggtattat tcacaacatc ccagcctgca
    42121 ccttaagggc gactggctga aagaggccgg atttgatacc ggacggggtg tcacggtgaa
    42181 gatttcggag gggggcctga ccatcatcgc tgacagtgac gaggtgcagg aactgcggga
    42241 tgggatgttt agtgtgctga acgaaagtta aatattaaaa aagctgggaa gatttaaaac
    42301 cttcctagct ttttattctg tcttattata atggatactc atcatcttcc gatgaattta
    42361 taaattgctc caagtttaca tgaagagtac ttaacctagt ttttaactca ttaagtgaag
    42421 cgataatttc atattttcca acataatcat atgatggatc gtattcatta tctatgtcag
    42481 aagctaaaaa tgatgatatt cgtagtttaa atttagcttc atctttatta cattcattgg
    42541 cataaggtga atgccctatc atagcatatt catctaatct aaaaggataa gatggtttta
    42601 attcatcgat caatgaaatt tcaatccatg ttctatatcc aggcattaat ggactaattt
    42661 ctttgagcaa aacctttcca cttaataatg tattgagatt gttcattatt atgtctccca
    42721 cgggaaaaat atatgtctaa cacttccacc atgttgatga acttgtaaat gtggtaaatc
    42781 accatcagga tttcctagtc catgacctga cactctatat gaaggttttg ttacagaagg
    42841 atcttttata tcatcccaat gatatgaacc tgcttttgtt ttatttccag taagatattc
    42901 cattaagttc ttagtgctgg ggcctgactc acctgtcata tttttataat tatgacctaa
    42961 atatctatcc ataaacaatt gttcagcgtc acttctagac ctaacagtaa ctttggtttc
    43021 actactatct aataaacgat tcgctttttg gttactaaca gcacatggag ttaatccaag
    43081 tggatcagca tattttagag gattaggtgc ataactgtag agatttaaac cgcctctcaa
    43141 ccctatcggg tcagttacca tgaaccgccc gacatccgga tcatagtacc tgtgcagatt
    43201 gtagtgcagc cccgtttccc tgtcgaggta ctgtccctgg tagcgcaggt tctgctccac
    43261 cccgccgtgg tccttctcct gtatcgtgtt accccagacc tgataccccg tctcccacac
    43321 gattttcccg tcgctgtccg tcatctcttc cggcgcgccg ttcacatccg tgtgaaaata
    43381 tagtatccgg ttcgggccct ctttgcccgc cttatccacc cgcgccagcg gttcataact
    43441 cccctggtcc ctgtagatgt acaggctgcc tgttccctcc ggcctgctct cctgcgccag
    43501 ccgcattccg tcccataaaa attttgtccg gttgtacggc ttaccgtcag ggctgatgtg
    43561 ctgtttttcc gtccgcctcc ccagtgcatc gtacacatac cggtatgtcc cccgcgctgt
    43621 cgtcacttcc accatccggt gttccgcatc ataacggtaa ctctgcgttc cgctccggcc
    43681 ctccttttcc accgcacggc caaattcgtc ataacggtag tgcacaccac ggtaagacat
    43741 cagccggttg aacggcacca cgctgttttc tgccgtggcc ctttcccccg cgcgcctccc
    43801 cagcaggttt cccgccgcgt cgtactgcca gctcgccaga tacccctcat cctccgaacg
    43861 acacgccgtg atgcgccccg tggggtcgta gaagtaatcc gtttcccccc gccggctgtg
    43921 ccgcgtcttc agcagttcat cctcgccact gtagctgtac tgccggtccg tgaacgtctc
    43981 cggtaccacc ccgcgcatcc cgctgtatgt ctccctgcgc ttcagccgcc cgccggggtc
    44041 gtaaagctgc cgcgtgctca gtcgcccctg gcttctgagc acttcacggt gcaggtggtc
    44101 gcgcgtgaac tcgctgatga cctcccggtc cagatttatc tgctggagat ggccgctgcc
    44161 gtagtacaga tggcgctgcg ttctgccgtc cggaaaggtg gtggcagtcc ggttgcccag
    44221 tttatcgtac tggtgggtca ggatgccctg cgccgtctcc tcgctcagca tccgccccag
    44281 tctgtcgtag ctgaagcgga taacttccgg cgcgtcagtt tcgtgccgct ggcgacggat
    44341 ttccagtagc tggtctgccg catcatggac gtattccgta cggccatcgg ctgtctcttt
    44401 tgccgtgagc cggcccgccg catcacgcgc cagccggtgg cggatttcac cgccccggtc
    44461 cccggtgaac accacttccg tgaccgcgtt cagcgcgttg tagcggtacg actggcggca
    44521 gccgtccggg tttatctgct ccgccagacg gtccagcaca tcatggcgga accggtagcg
    44581 ttctccgttc tcgttcgtga gcgtgaccag acgcccgtaa gcgtcgtacc cgaatgccgt
    44641 cctgcgcccc atcgcgtccg tccggctgcg cacccggccc tgcccgtccc gctgccagcg
    44701 cgttatcctc cccatcgggc tggtgtactt taccagcctg cccgccgcgt ctgactggta
    44761 attcaccgtg cggccgtccg ccagtatcac ctccgtcaga tgaccgctgt cgtcataccc
    44821 gtagcgcgtg ctgtggctct gcgcatccgt cattctgata agcctcgtgc gttcgtcgta
    44881 gaaccacgcc gtctgcgagc cggaacagtc cgtgtgccgc atgagctgcc cgtcttcatt
    44941 ccactggagg tatttattcc ccccgcgcgc atccgttatc tgcaccacct agccgtggtg
    45001 gtcatactga taccgggtgt tctgctgcag cgggtcggtc acggcaagaa ggttgccgcg
    45061 ttcatcgtac acacagcgcc aggtgtttcc cgccgcgtcc acctccgttt ccggctggtg
    45121 ccacaccggg tgccactgcg cctgctccac ccgccccagc gggtcgtgcg tctccgtgat
    45181 atgcccctgc aggtcataca catagcgcca tttgccgccc tgagggtcgg tcatccccag
    45241 caacagtcgt tcttcgtcgc tccagcggaa tgtcgtcacc tgtccggctt cgtcctggta
    45301 cgccgtcacc tgattctgcg catcccactg gtgataacgt gtgcgcccca gtccgtccgt
    45361 cacgcacagc ctgcgctgcg ccaggtccca cgccagccgg tactgttcac cgctgtccgt
    45421 gtggtgcccc gtcacccgcc atgccgcgtc atccggacct tcataccagc cccactggta
    45481 ataataccct tcgccgcccg gctggcggtg cagcgtcatg cagtggtttt cgtcgtaagc
    45541 aaactcacgc agcaggtgcc ccgtgctgtc gtacacgccg ttcaggtggc cggcctcgtc
    45601 atagctgtaa cgacgcagca gtaaaggtgc agtctcaggg aaatgctgat aaatctgtgt
    45661 cacccgccgg ggatgcgccg ccagttcgta atacagacgg atgcacggac gctgctgttc
    45721 gccgctgatt tccacaatgc ggccccggtc gtcgtagtac agattcagac agttgctgtt
    45781 acggtcgccc agcattttca gccgctggcg ctgcgggtgg tgcgggtcag cctcaaacag
    45841 aaaacactgc ccgtcatcgc tgctgataag ccagtgcccc tgctcaccac gccttaccgc
    45901 cagcccttca ccggggctgt aaaacccgcc ccccggcagt acggcttcca tatccaagcg
    45961 tcgccccatg cccgacacaa aggtcatgca gttttcgtct gcgctacccg gtgtgcacac
    46021 gaggctgact ttatacatta cactccagcc ctgaccaaac attccttccg tacgttcgtc
    46081 acgactgctg tacacgcgct gccagtccag cggaaagtgg cccggcagac tgaaatcgaa
    46141 gtcatcgtca tcggccaggt atttggcccc ggtggcggca ttgaccggat gcggaagggc
    46201 actggtggcc ttctgcgtca gcatgctcat cccgacactg gcagcaaaac aggcgatttt
    46261 agaaagcatt ttgcccgggc gaagaaacat cagcgccacg gttgccacca gggttatctg
    46321 cgacctgccg cttctgatat ccctgaccac cagtaacgga ccgccaatgc gcacatccgg
    46381 tgacacaaac gccccgttcc ccggctcatt caccaccctg gcctcgcagg tacagcgtgc
    46441 cccgctgcgt gtcgccggct ggctgttgat acgcacatcc cgcgcgcctt ccgcgatata
    46501 ttccgctgcg ggcggctcgt catcgccttt gaacagatta ctgatgctgc caataaccgc
    46561 attcactccc tgaaaggcgg tttccagcat accgcccgga ttcagcaggt taagggccgt
    46621 tcccaggggg tcatccagaa aggcggcttt tttttgtgcg agaaactgcg tcagtgagga
    46681 aggatgttta tggcagtcga ttttatcgtc aggggtcggg gtggatcccg gggccgcact
    46741 ggccacagtg ggctgccata gttccgacac aaaatgtgtc acgccatcca ccatttcaga
    46801 acgcccttct tccgtactga gctggtcgtg cagagcccgg gacatcagca caggccctgt
    46861 caccgggtta accgccaggg ctatggcccc cgccatttcc agatattccc cggccacatc
    46921 ccggaacgtt ctggcatcct cctgacgctg cgcttcttcc tgttctgcct tcgcctgcgc
    46981 ttcctgcgcc tctatgtcct gccgcgatgc cgcagtcgcc gctgcccgcg cggcaggttt
    47041 gctgttgatc catgtgtcac cagagccggt gaggatgtaa ccttcaatct gaggcgggaa
    47101 aagcatattt gcgagggctg aacaggcttc gctgaccttt tctgtcacgc cggtgtacac
    47161 catcgccaca cccgccacaa aaccggtcag cacgacggcc gtgcacccca tcgtgatccc
    47221 cactgacgtc gccagtaacg aggttgtgcc gacaatgagg gcatcaataa cgacatttgc
    47281 ggccacctcc agcaccgccc ccagcacatc tgccataaac gacgtatgca gcagggcatc
    47341 accctgtctt gccgcccaga atgcctcttc catgtcccgt tccctgtatg attaattcgt
    47401 cctgacttcc tgccgcatcc tgaagcgcag attcttttta atttcagccc agcgcgtcat
    47461 atctgccggg gtcagcggtg tggttctggc gtaggtgaag gccatcatga cccgctcacc
    47521 cggcaataca aatgccacct gacactggta cacctgctcg ttaccccgga gaaactggct
    47581 ctgtatttcc aaaccgcctc gttgagataa tattccgtca taatgtaaat agtccgtttt
    47641 aataaaggaa ttatttattg acaggttctg tttttttaat catgaatg
//
LOCUS       DAJVRL010000020        47341 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVRL010000020 DAJVRL010000000
VERSION     DAJVRL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.020"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OQN02_001111"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OQN02_001111"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS1641985.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="OQN02_001112"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="OQN02_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1641986.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="OQN02_001113"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="OQN02_001113"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1641987.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="OQN02_001114"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="OQN02_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1641988.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="OQN02_001115"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="OQN02_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1641989.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="OQN02_001116"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="OQN02_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1641990.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="OQN02_001117"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="OQN02_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1641991.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="OQN02_001118"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="OQN02_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1641992.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="OQN02_001119"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="OQN02_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1641993.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="OQN02_001120"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="OQN02_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1641994.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="OQN02_001121"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="OQN02_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1641995.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="OQN02_001122"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="OQN02_001122"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1641996.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="OQN02_001123"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="OQN02_001123"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1641997.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="OQN02_001124"
     CDS             11233..12087
                     /locus_tag="OQN02_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1641998.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="OQN02_001125"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="OQN02_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1641999.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="OQN02_001126"
     CDS             complement(13550..14689)
                     /locus_tag="OQN02_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1642000.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="OQN02_001127"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="OQN02_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1642001.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /locus_tag="OQN02_001128"
     CDS             15875..17236
                     /locus_tag="OQN02_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1642002.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /locus_tag="OQN02_001129"
     CDS             17233..17751
                     /locus_tag="OQN02_001129"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1642003.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="OQN02_001130"
     CDS             17751..18071
                     /locus_tag="OQN02_001130"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1642004.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="OQN02_001131"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="OQN02_001131"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1642005.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /locus_tag="OQN02_001132"
     CDS             18890..20092
                     /locus_tag="OQN02_001132"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCS1642006.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="OQN02_001133"
     CDS             20189..20611
                     /locus_tag="OQN02_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1642007.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="OQN02_001134"
     CDS             complement(20659..21531)
                     /locus_tag="OQN02_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1642008.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21543..22637)
                     /locus_tag="OQN02_001135"
     CDS             complement(21543..22637)
                     /locus_tag="OQN02_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1642009.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="OQN02_001136"
     CDS             complement(22670..23668)
                     /locus_tag="OQN02_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1642010.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="OQN02_001137"
     CDS             complement(23693..25204)
                     /locus_tag="OQN02_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1642011.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="OQN02_001138"
     CDS             complement(25227..26210)
                     /locus_tag="OQN02_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1642012.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29408)
                     /locus_tag="OQN02_001139"
     CDS             complement(26307..29408)
                     /locus_tag="OQN02_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCS1642013.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29415..29588)
                     /locus_tag="OQN02_001140"
     CDS             complement(29415..29588)
                     /locus_tag="OQN02_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642014.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29706..30899
                     /locus_tag="OQN02_001141"
     CDS             29706..30899
                     /locus_tag="OQN02_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCS1642015.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="OQN02_001142"
     CDS             complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="OQN02_001142"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1642016.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32677..33867
                     /gene="hmpA"
                     /locus_tag="OQN02_001143"
     CDS             32677..33867
                     /gene="hmpA"
                     /locus_tag="OQN02_001143"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1642017.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="OQN02_001144"
     CDS             complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="OQN02_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1642018.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="OQN02_001145"
     CDS             complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="OQN02_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1642019.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQVLEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="OQN02_001146"
     CDS             complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="OQN02_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1642020.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="OQN02_001147"
     CDS             complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="OQN02_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1642021.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38516..42403)
                     /gene="purL"
                     /locus_tag="OQN02_001148"
                     /gene_synonym="purI"
     CDS             complement(38516..42403)
                     /gene="purL"
                     /locus_tag="OQN02_001148"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1642022.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42662..44218
                     /gene="mltF"
                     /locus_tag="OQN02_001149"
     CDS             42662..44218
                     /gene="mltF"
                     /locus_tag="OQN02_001149"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1642023.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="OQN02_001150"
     CDS             complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="OQN02_001150"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1642024.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="OQN02_001151"
     CDS             complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="OQN02_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1642025.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45620..46468
                     /locus_tag="OQN02_001152"
     CDS             45620..46468
                     /locus_tag="OQN02_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1642026.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46807..47341)
                     /locus_tag="OQN02_001153"
                     /pseudo
     CDS             complement(46807..47341)
                     /locus_tag="OQN02_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11289 a        12962 c        11901 g        11189 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgcgcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ttgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag ccttcgatta cgccgccctg
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tgataacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaagaga
    30241 agctgggcat tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    30301 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    30361 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    30421 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg
    30481 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    30541 cggttaatac tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    30601 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30661 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30721 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30781 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    30841 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30901 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    30961 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    31021 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    31081 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    31141 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    31201 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    31261 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    31321 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    31381 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgct
    31441 cgaggaacac ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga
    31501 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    31561 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    31621 cacctttcgc caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    31681 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    31741 cgtgcgccat atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg
    31801 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    31861 ctttggcttg tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag
    31921 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    31981 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    32041 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    32101 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    32161 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    32221 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    32281 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    32341 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    32401 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    32461 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    32521 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    32581 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    32641 agatacatca attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
    32701 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
    32761 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
    32821 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
    32881 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
    32941 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
    33001 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
    33061 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
    33121 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
    33181 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
    33241 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
    33301 actcgcaaac cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag
    33361 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
    33421 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt
    33481 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
    33541 caagtgaact ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt
    33601 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
    33661 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
    33721 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
    33781 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
    33841 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
    33901 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga
    33961 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
    34021 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
    34081 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
    34141 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
    34201 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
    34261 gaaaaggttg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
    34321 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
    34381 cccgccattc tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
    34441 tagttgagtt caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
    34501 tccagcacct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
    34561 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt
    34621 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
    34681 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
    34741 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
    34801 tttgacagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
    34861 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
    34921 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
    34981 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
    35041 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
    35101 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
    35161 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
    35221 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
    35281 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
    35341 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
    35401 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
    35461 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
    35521 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
    35581 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
    35641 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
    35701 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
    35761 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
    35821 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
    35881 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
    35941 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
    36001 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
    36061 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
    36121 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
    36181 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
    36241 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
    36301 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
    36361 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
    36421 aaccggggcg tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa
    36481 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
    36541 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
    36601 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
    36661 cgctggtggc ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg
    36721 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
    36781 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
    36841 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
    36901 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
    36961 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
    37021 tgttcgatca gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt
    37081 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
    37141 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    37201 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    37261 ctcggtccac agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    37321 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    37381 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    37441 tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg
    37501 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    37561 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    37621 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    37681 taaaccttcg caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    37741 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    37801 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    37861 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    37921 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    37981 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    38041 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    38101 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    38161 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    38221 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    38281 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    38341 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    38401 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    38461 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    38521 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    38581 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    38641 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    38701 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    38761 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    38821 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    38881 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    38941 gccacaactc actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    39001 atacccccag ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    39061 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    39121 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    39181 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    39241 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    39301 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    39361 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    39421 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    39481 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    39541 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    39601 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    39661 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    39721 ccagcgttac cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    39781 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    39841 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    39901 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    39961 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    40021 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    40081 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    40141 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    40201 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    40261 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    40321 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    40381 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    40441 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    40501 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    40561 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    40621 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    40681 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    40741 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    40801 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    40861 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    40921 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    40981 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    41041 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    41101 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    41161 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    41221 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    41281 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct
    41341 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    41401 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    41461 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    41521 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    41581 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    41641 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    41701 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    41761 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    41821 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    41881 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    41941 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    42001 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    42061 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    42121 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    42181 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    42241 gttgtgcgtg ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg
    42301 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    42361 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    42421 agctttcggt gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    42481 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    42541 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    42601 acctttcact attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta
    42661 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    42721 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    42781 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa
    42841 cgggaaacct tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt
    42901 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg
    42961 taacgccgac ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca
    43021 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    43081 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    43141 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    43201 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    43261 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    43321 gctcgatatc accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac
    43381 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    43441 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    43501 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    43561 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    43621 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    43681 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    43741 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc
    43801 cctcgctgcg tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa
    43861 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    43921 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    43981 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    44041 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    44101 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    44161 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    44221 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    44281 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    44341 catacccgga tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg
    44401 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    44461 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    44521 accctgccgc agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg
    44581 gttccagcct tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc
    44641 ccgctcatcc caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct
    44701 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    44761 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg
    44821 atgctgacag aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag
    44881 ccgcagcggc gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg
    44941 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg
    45001 cggcagccag ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat
    45061 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    45121 aaaggcggta acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg
    45181 tgcttcgctg tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc
    45241 acgacctttt atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc
    45301 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    45361 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    45421 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    45481 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    45541 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    45601 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct
    45661 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    45721 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    45781 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    45841 ggcaagccag ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc
    45901 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    45961 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    46021 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    46081 gatgaagatt ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt
    46141 acaggcgtcg tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga
    46201 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    46261 ctttactccg aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    46321 acaagcgaca aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt
    46381 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    46441 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    46501 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    46561 atatgtgtga aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt
    46621 gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa
    46681 caccactttc tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc
    46741 tgtgacatac aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct
    46801 gcggttttat ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata
    46861 gatgatcgcc accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca
    46921 ttattctggt gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc
    46981 cagtcctgct tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat
    47041 cacatggaag aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg
    47101 aaagttgatg cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg
    47161 ccccagccga tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag
    47221 ttttttcagc cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc
    47281 gcatcctgca cagttcaacg cattacgttg tagatcggta ttctggtcat ttgttgatac
    47341 g
//
LOCUS       DAJVRL010000021        47147 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVRL010000021 DAJVRL010000000
VERSION     DAJVRL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.021"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..871)
                     /gene="rpnA"
                     /locus_tag="OQN02_001154"
     CDS             complement(<1..871)
                     /gene="rpnA"
                     /locus_tag="OQN02_001154"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCS1642027.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
     gene            complement(1074..1310)
                     /gene="feoC"
                     /locus_tag="OQN02_001155"
     CDS             complement(1074..1310)
                     /gene="feoC"
                     /locus_tag="OQN02_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1642028.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1310..3631)
                     /gene="feoB"
                     /locus_tag="OQN02_001156"
     CDS             complement(1310..3631)
                     /gene="feoB"
                     /locus_tag="OQN02_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1642029.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3648..3875)
                     /gene="feoA"
                     /locus_tag="OQN02_001157"
     CDS             complement(3648..3875)
                     /gene="feoA"
                     /locus_tag="OQN02_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1642030.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4313..6634)
                     /locus_tag="OQN02_001158"
     CDS             complement(4313..6634)
                     /locus_tag="OQN02_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1642031.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6731..7207)
                     /gene="greB"
                     /locus_tag="OQN02_001159"
     CDS             complement(6731..7207)
                     /gene="greB"
                     /locus_tag="OQN02_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1642032.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7435..8154
                     /gene="ompR"
                     /locus_tag="OQN02_001160"
     CDS             7435..8154
                     /gene="ompR"
                     /locus_tag="OQN02_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1642033.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8151..9503
                     /gene="envZ"
                     /locus_tag="OQN02_001161"
     CDS             8151..9503
                     /gene="envZ"
                     /locus_tag="OQN02_001161"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1642034.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9579..11201)
                     /gene="pckA"
                     /locus_tag="OQN02_001162"
     CDS             complement(9579..11201)
                     /gene="pckA"
                     /locus_tag="OQN02_001162"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1642035.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11580..13304
                     /locus_tag="OQN02_001163"
     CDS             11580..13304
                     /locus_tag="OQN02_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1642036.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(13448..14317)
                     /gene="hslO"
                     /locus_tag="OQN02_001164"
     CDS             complement(13448..14317)
                     /gene="hslO"
                     /locus_tag="OQN02_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1642037.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14342..14743)
                     /gene="hslR"
                     /locus_tag="OQN02_001165"
     CDS             complement(14342..14743)
                     /gene="hslR"
                     /locus_tag="OQN02_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1642038.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14754..15422)
                     /gene="yrfG"
                     /locus_tag="OQN02_001166"
     CDS             complement(14754..15422)
                     /gene="yrfG"
                     /locus_tag="OQN02_001166"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1642039.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15487..17622)
                     /gene="igaA"
                     /locus_tag="OQN02_001167"
     CDS             complement(15487..17622)
                     /gene="igaA"
                     /locus_tag="OQN02_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1642040.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17942..18502
                     /gene="nudE"
                     /locus_tag="OQN02_001168"
     CDS             17942..18502
                     /gene="nudE"
                     /locus_tag="OQN02_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1642041.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18667..21219)
                     /gene="mrcA"
                     /locus_tag="OQN02_001169"
     CDS             complement(18667..21219)
                     /gene="mrcA"
                     /locus_tag="OQN02_001169"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1642042.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21339..22118
                     /gene="hofM"
                     /locus_tag="OQN02_001170"
     CDS             21339..22118
                     /gene="hofM"
                     /locus_tag="OQN02_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1642043.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22118..22657
                     /gene="hofN"
                     /locus_tag="OQN02_001171"
     CDS             22118..22657
                     /gene="hofN"
                     /locus_tag="OQN02_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCS1642044.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22641..23081
                     /gene="hofO"
                     /locus_tag="OQN02_001172"
     CDS             22641..23081
                     /gene="hofO"
                     /locus_tag="OQN02_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1642045.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23071..23475
                     /gene="hofP"
                     /locus_tag="OQN02_001173"
     CDS             23071..23475
                     /gene="hofP"
                     /locus_tag="OQN02_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS1642046.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23387..24625
                     /gene="hofQ"
                     /locus_tag="OQN02_001174"
     CDS             23387..24625
                     /gene="hofQ"
                     /locus_tag="OQN02_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1642047.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25026..25547
                     /gene="aroK"
                     /locus_tag="OQN02_001175"
     CDS             25026..25547
                     /gene="aroK"
                     /locus_tag="OQN02_001175"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1642048.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25604..26692
                     /gene="aroB"
                     /locus_tag="OQN02_001176"
     CDS             25604..26692
                     /gene="aroB"
                     /locus_tag="OQN02_001176"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1642049.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26784..28070
                     /gene="damX"
                     /locus_tag="OQN02_001177"
     CDS             26784..28070
                     /gene="damX"
                     /locus_tag="OQN02_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1642050.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28177..29013
                     /gene="dam"
                     /locus_tag="OQN02_001178"
     CDS             28177..29013
                     /gene="dam"
                     /locus_tag="OQN02_001178"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1642051.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29031..29708
                     /gene="rpe"
                     /locus_tag="OQN02_001179"
     CDS             29031..29708
                     /gene="rpe"
                     /locus_tag="OQN02_001179"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1642052.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29701..30459
                     /gene="gph"
                     /locus_tag="OQN02_001180"
     CDS             29701..30459
                     /gene="gph"
                     /locus_tag="OQN02_001180"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1642053.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30452..31456
                     /gene="trpS"
                     /locus_tag="OQN02_001181"
     CDS             30452..31456
                     /gene="trpS"
                     /locus_tag="OQN02_001181"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1642054.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31726..32631
                     /locus_tag="OQN02_001182"
     CDS             31726..32631
                     /locus_tag="OQN02_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642055.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32648..33010
                     /locus_tag="OQN02_001183"
     CDS             32648..33010
                     /locus_tag="OQN02_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1642056.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33094..34257
                     /locus_tag="OQN02_001184"
     CDS             33094..34257
                     /locus_tag="OQN02_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCS1642057.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34258..35484
                     /locus_tag="OQN02_001185"
     CDS             34258..35484
                     /locus_tag="OQN02_001185"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1642058.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35481..36359
                     /locus_tag="OQN02_001186"
     CDS             35481..36359
                     /locus_tag="OQN02_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1642059.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36370..36723
                     /locus_tag="OQN02_001187"
     CDS             36370..36723
                     /locus_tag="OQN02_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1642060.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36735..38039
                     /locus_tag="OQN02_001188"
     CDS             36735..38039
                     /locus_tag="OQN02_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1642061.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38051..39136
                     /locus_tag="OQN02_001189"
     CDS             38051..39136
                     /locus_tag="OQN02_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1642062.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(39296..40027)
                     /gene="phoB"
                     /locus_tag="OQN02_001190"
     CDS             complement(39296..40027)
                     /gene="phoB"
                     /locus_tag="OQN02_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1642063.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40127..40912)
                     /gene="frlD"
                     /locus_tag="OQN02_001191"
     CDS             complement(40127..40912)
                     /gene="frlD"
                     /locus_tag="OQN02_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1642064.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40909..41739)
                     /gene="frlC"
                     /locus_tag="OQN02_001192"
     CDS             complement(40909..41739)
                     /gene="frlC"
                     /locus_tag="OQN02_001192"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCS1642065.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(41789..42811)
                     /gene="frlB"
                     /locus_tag="OQN02_001193"
     CDS             complement(41789..42811)
                     /gene="frlB"
                     /locus_tag="OQN02_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1642066.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42832..44220)
                     /gene="frlA"
                     /locus_tag="OQN02_001194"
                     /pseudo
     CDS             complement(42832..44220)
                     /gene="frlA"
                     /locus_tag="OQN02_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(44464..44631)
                     /locus_tag="OQN02_001195"
     CDS             complement(44464..44631)
                     /locus_tag="OQN02_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1642067.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(44878..46251)
                     /gene="cobA"
                     /locus_tag="OQN02_001196"
     CDS             complement(44878..46251)
                     /gene="cobA"
                     /locus_tag="OQN02_001196"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1642068.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(46270..47076)
                     /gene="nirC"
                     /locus_tag="OQN02_001197"
     CDS             complement(46270..47076)
                     /gene="nirC"
                     /locus_tag="OQN02_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1642069.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
BASE COUNT        11054 a        12010 c        12816 g        11267 t
ORIGIN      
        1 tcgccgcagc caactcttct tctgacagcc cggtaaatcg catgatgtct gcaagaggga
       61 ctccggattc aagcattatt ttggctatat gcagggcttt ggattgttcc ccctcctgcc
      121 gcaatctttc cgcaatagtc attaaactct ccttgtgttt cggtgaacgt tcggcaacgc
      181 cgtcgataaa atcgttaaaa cgtacagcgt cgccagtttg cagtatgtaa ttaaacagcc
      241 ctttgatttg tctgtcatta gcgtatccac tacttaataa gcaggccatt tgctctacca
      301 gccccatcag atcgcgttga cgaatatgtt tttgaattaa ctccagcagc gccatacgtc
      361 ggtgctgcat gatttcatca tcaggcatga cggtgacatc aatcagcgga aatgcggagg
      421 cataaagctg ccttgccagt ttgggatcgg cgaaacaatc cagccaacac agcgaatagg
      481 gataggggct ttcaataccg tggtaaaaca acaatggcac caccatcggc aacgttttgt
      541 atccagcatc aaggtgattt tgcattgcgg caatagcgta acgcatcatg cgaaatgcga
      601 tcagtttgtt tgaggtgctt tgatgttcaa tcagacaata gatgtatcct ggtccttgtt
      661 ccgttttcac cgaccacagc acatcggaat agctttcacg cagatcgtca tcaataaagc
      721 tcctcgactc cagcttgagg gttttcatat cacagagcgc gtgaatgggt gccggtaaat
      781 gaaacgcaag aaaatcgcgt gccgtctccg gttggcgtaa aaagagtttg aacagcgcat
      841 catgtggggt ggaactctgc tttttactca tggcgttcct tcacctgttg tttgaatgcc
      901 gcgactctac gcgccactcc acatctgtgc attagcgagt tttcatccct gcgagacgct
      961 ttcagaggaa cttacgttgc gctgcaagaa ttgcattttt atggaaagcc gcacgcaaaa
     1021 aagcccggat gcaaacgcat accgggcttt tcaaaacagg cgatggagta tggttaacgc
     1081 agcgcccacc actcgcgcag acaggctttg ccttccgggc agcttttaca actgccagag
     1141 aggcagccgt caggttcttc ctgaatccgc acggctttgc ccatgctttc cagctgttgc
     1201 agcatggcgt taatcattgg ctgcggagtg ttcaatgtct ggcttatctg ggtcgcttcc
     1261 atacggcccc gtaacgccag caaatcacgt acctgaataa gtgaagccat taatggcaat
     1321 caccggtggt gctggctgcg cagcaactgc ttaccgactt gcgggttgcc agcagttcga
     1381 tatccacccg gctacgcgcg cggcgcagca gaccgatagc tacgatgtta aacaggatta
     1441 ccgccagaat gcacaccagg ctgtaagttg ggtgctgact gtagctggcg acctgataga
     1501 acaatgttgc cagtgagtaa gcgatattca gcccccacag gatggagaag cccatccagc
     1561 cacggcttga ttcacgggcg atggctccca tcaccgagat acatggtaca tacagcagga
     1621 cgaaaatcag gtagctgtat gcggcagctg cgctaccgaa tttctgatcc atcacgccca
     1681 tcgccccggt acccatttca ccgttgcctt tgctagcttc aatggggttc atcagtacgc
     1741 taaggctgaa ggtgtctttc aggctctgcc aggtttcatc taccgcactg aacagctctt
     1801 caccgaggtt gaattccgcc ggattgaact cttcgtcctg aatattttct gcggtgtaga
     1861 gggtgttgag cgtacccacc accacttctt ttgccattgc gcccgtaaac aggccaaccg
     1921 ttgcctgcca gttatcttca tgtacgccaa tcggcttgaa gacaggggtt atcacccggc
     1981 tgacggaagc cagcgccgag tcgttgatgt tatcgacgat tttcccgctc aacgagaagc
     2041 tgttgaaagc gctcaggaaa atgctgacga tgataatcac tttaccagca cgcagaacga
     2101 agcccttcag acgctgccag gtctggataa tcaggctttt aacgtgtggt acatggtaga
     2161 ccggcagctc catgacaaac ggcgtcgctt caccgcgcat gatggtgtac ttgagcatca
     2221 ggccagtcag caccgccatc acgataccga gcatatacag cgagaaaacc gccagggcac
     2281 cgttctgccc gaagaaggca gccgcgaata ctgcgaagat agccagacgc gcgccgcagg
     2341 acataaacgg tgccatcatg atggtcatca gacgttcacg cggtgcatca agcgtacgtg
     2401 cgcccattac cgacggtacg ttacaaccga aaccgacgat cagcggcaca aaggatttcc
     2461 ccggcaagcc cagcgcctgc atcaggcggt ccatcacaaa cgctgcacgc gccatatagc
     2521 cggagtcctc aaggaaggag aggaacaggt acatcatgcc gatctgcggc accagtggca
     2581 gcacggtgtt aataccgcca cccaggccct gggcgaggaa gatagtcagc cagtccggga
     2641 agtggagcgt gtagccaatc cattgaatac catgcacaaa tagcgccacg gagccgacgt
     2701 taaacagcgg ctgtaacgcc ccgccgatgt tgatagccag caggaacatc aggtacatca
     2761 caaagaggaa aatcggcaga ccgaggaaac ggttgagcac gattttatct accgcagtgg
     2821 tgaaacggct gggttctgcc gtcagggtgt tgcttaccac atcacagatg gcagcaatgc
     2881 actggtaacg cgcatcggca atgtgcagcg ccggatcatc catttcatta cgcagacggg
     2941 cgagggtggc atccagatgc tgcgacgctt caccggcgta ggcgcggctg tagatatcgc
     3001 cttccagcat ttgcaggccc agccagcgac gttgtttcag cgggatgtcg gaaggcatca
     3061 cttttgccag tgaatctgct tcgttgagta gcggctgtgc gtaatgcacc agttccacat
     3121 tctcgttagc tttatagcga tcaatcgcca gcttgagcgc ttcaataccg cgaccacggg
     3181 ttgaaaccag cgggatcacc gggcagccaa gacgtgccga tagagcatca atttcaatac
     3241 gaatattttg cttctcggca atgtcgagca tgttcagcgc cacgatgcag ggaatgccga
     3301 gttccagcag ttgcagcgtc aggtacaggt tacgctcaag gttagacgca tccaccacgt
     3361 taatcagcag gtcggcgtcg ccactcaaaa tgtagtgaca ggcgatttgc tcatcgagcg
     3421 aggtctgcga tgagatggtg gtcagagaat aggtgccggg caggtccacc agcgtgacct
     3481 gatgatcggt ggtggagaat tgcccttctt tacgttcgac ggtaacgcca gcccagttac
     3541 ctacacgctg acgtgcgcca gtgagctggt taaataaggt tgtcttgcca gaatttggat
     3601 taccaattaa gccaatggtt aattttttca ttgttgttat caccgtatta acaggaaacc
     3661 gcttccactt ctaataaggc cagatctttt ttgcgtaata ccaggctcac acgacgggtt
     3721 tcgatatgaa tggggtcgcc gagtggagcg acgcgcacca catcaaaaga ggagccaggt
     3781 aacatgccta gagaaagcag tttttggcga tatgccgggc tgatttcacg ggaaaagcca
     3841 gtgattttcc acgcagtatc tggagtgtat tgcataggtg cctacttgtt tctcattaac
     3901 tggataacta cctcatgggg cgtttgaccg cgacatgaga ccagatgatt tgccaacaaa
     3961 ggagagatta aataatcaac aacacaatga taatgagaat ggtttctatc agcaatactt
     4021 aaaatgtgtg cgaatgttgg tttgaataat aatctgactc aatgttgatg tggctcaagg
     4081 ttatatcgca gcgaaaatat aattcgaata gtgagttacc cggactggct aatgtttaat
     4141 taaggtttca gttgttaatg aagtgataaa tattaacgga gtatattgtg agtttggctg
     4201 tggaataaaa gtgccggaga tattctccgg cacttttatg tcaggcaaag cgttcgttgc
     4261 cagatgcggc gtgaacgcct tatcgcgcag gtttaggggc cagtccgggc gtttaacgtt
     4321 ttttacccat cgctgccgcc agcgcatcca tcatcgcgct attaccggca ggctgcgctt
     4381 cacgaccgcg tggtttggct gccgggcggt tgttttgcgg gcgttcatta ccgccgccgc
     4441 gacgagagtt ggtttcgcca ggctgctcgt ccagacgcat agtcagggcg atacgtttac
     4501 gctgaagatc cacttccagc actttcacct tcacaatgtc gcccgctttc accacggtat
     4561 gcggatcttc cacaaacttg ttcgataatg aagagatgtg aaccaggccg tcctgatgca
     4621 cgccaatatc gacaaacgcg ccaaagttgg tgacgttggt cactgcgcct tcgaggatca
     4681 tacccggttg caggtcgttc attgtctcga cgccatcggc aaactgagcg gttttaaatt
     4741 ccggacgcgg atcgcgaccc ggtttttcca gctctttgat gatgtcagtt actgtcggca
     4801 caccgaattt ttcatcagta aagtcagacg ctttcaggtt acgcagttcg ctgctgttac
     4861 ccatcagatc tttcagtgcc tgctgtgttg ctgccagaat gcgttccacc accggatagg
     4921 cttccggatg aacggtagag gcgtccagcg ggttatcacc gtggttaatg cgcaggaagc
     4981 ccgcgcactg ctcgaaggct ttcggcccca gacggctgac tttcagcagt tgctgacggt
     5041 tctggaactg gccgttctca tcgcgccagg ccacgatgtt ttgcgccatc atgcgcgtca
     5101 ggcccgccac gcgggttaat agcggaacag aagcggtgtt gagatcgacg ccaacggcgt
     5161 ttacgcagtc ttctactact gcatccagtt tgcgggccag ttgcgtctgg ctgacgtcat
     5221 gctgatactg acctacgccg atagatttcg gatcgatttt caccagctcc gccagcggat
     5281 cctgcaaacg gcgggcgata gataccgcac cacgcagcga aacgtcgaga tccgggaact
     5341 cctgcgctgc cagctcggaa gctgagtaaa ccgacgcgcc agcttcgctg acgatcactt
     5401 tctgtgcggt cactttcggg aactgcttct gcacgtcgag atagaaacgt tcagtttcgc
     5461 gggaagctgt accgttgccg atcgccacca gttcaacgtt atgtttttca cacaaggcag
     5521 caacggtcat cgctgctttt gcggcttgtc cggtgtgcgg gtaaatggta tcggtcgcca
     5581 ccagtttgcc agtggcatcg accaccgcca cttttacccc ggtacgcaga cccggatcga
     5641 ggcccatcgt tgcacgcagt ccggcagggg ccgccatcag cagatcgtgc aggttacggg
     5701 caaagacgtt gattgcttca tcttccgcgc gttcgcgcac ggtacccatc agttcggttt
     5761 ccagatgcat cagcaccttg atgcgccagg tccagctcac tacgcttttg cgccagctat
     5821 ccgccggggc attgttcagg cgcaggccaa ggtgatccat gatgatttgc tcgcaatagc
     5881 tctctttggg cggctcatcg aactgcggat cggcattcag cgaaagctgg agtacgcctt
     5941 cgttacgccc acggaacatc gccagcgcgc ggtgagaagg caccgtggac aacggttcgt
     6001 gatgatcgaa atagtcgcgg aatttcgccc cttcctcttc tttaccgctc accaccgtag
     6061 aaaccaaatg cgcgttcttc cacagataat cacgcacttt cgccagcagc gcggcatctt
     6121 cggcaaaccg ttccatcagg atatagcgcg cgccatccag cgcggctttg gtatctgcca
     6181 cgcctttatc ggcatcaaca tattgtgcag cggcgacttc tggcgtgtgt gacggatcgc
     6241 tccacagcag gtcagccaac ggctcaagcc ctgcttcaat ggcgatttgc ccgcgggtgc
     6301 ggcgtttagg tttgtagggc aggtagaggt cttcgagttc ggttttgctt agggtggcgt
     6361 tgatggcctt cgccagatca tcggtgagtt tgccttgctc ggaaatggat ttgaggatcg
     6421 cctgacgtct ctcttccagc tcgcgcagat agctcagacg cgtttccaga ttacgcagct
     6481 gcgtgtcatc cagaccgccg gtgatttcct tacgataacg tgcgataaac ggcacggtat
     6541 tcccttcgtc aagcaggcga acggcagcgt caacctgttc cgggcgcgcc tgaatttcac
     6601 ccgcaataat gcggcagaac gaatcattca tcatggtttg agttcatctt ttcggatcaa
     6661 aaatcagggg atagttatac ggactggctg gcaaaaatgc cagccatcgg caggaggtta
     6721 agactcttcc ttacggtttc acgtactcga tagcattaac ataccagttc gcttccccgg
     6781 caggggtatt caccaccgcc agatcgccga cttctttttt cagcaatgcg cgggccatcg
     6841 gggaatcgat agagatgtaa tctttacggc caaaaatttc atcgtagccg acaatacgga
     6901 aacggtgagt cacgccatcg tcgttttcaa tctccaccca cgcgccaaaa aatactttgc
     6961 cttcctgctg aggggagtaa tcgacgattt tgagattttc caggcattta gtgagatagc
     7021 gcacgcgacg gtcgatttca cgcagacgct ttttattata ctgatagtca gcattttcgc
     7081 tgcggtcgcc cagacttgcg gcccaggtca ccttttttgt gacctccggg cgttcttcac
     7141 gccagagata attaagctct tgtttgagtt tttcataccc ttcccgggta accaggggcg
     7201 ttttcatctc gttgattccc tttgtctgtt tgataatgcg cacattgggt ataacgtgat
     7261 catatcaaca gaatcaataa tgtttcgccg aataaattgt atacttaagc tgctgtttaa
     7321 tatgctttgt aacaatttag gctgaaattt ataccagatt tagctggtga cgaacgtgag
     7381 cttttttaag aatacacgct tacaaattgt tgcgaacctt tgggagtaca aacaatgcaa
     7441 gagaactaca agattctggt ggtcgatgac gacatgcgcc tgcgtgcgct gctggaacgt
     7501 tatctcaccg aacaaggctt ccaggttcga agcgtcgcta atgcagaaca gatggatcgc
     7561 ctgctgactc gtgaatcttt ccatcttatg gtactggatt taatgttacc tggtgaagat
     7621 ggcttgtcga tttgccgacg tcttcgtagt cagagcaacc cgatgccgat cattatggtg
     7681 acggcgaaag gggaagaagt ggaccgtatc gtaggcctgg agattggcgc tgacgactac
     7741 attccaaaac cgtttaaccc gcgtgaactg ctggcccgta ttcgtgcggt gctgcgtcgt
     7801 caggcgaacg aactgccagg cgcaccgtca caggaagagg cggtaattgc tttcggtaag
     7861 ttcaaactta acctcggtac gcgcgaaatg ttccgcgaag atgagccgat gccgctcacc
     7921 agcggtgagt ttgcggtact gaaggcactg gtcagccatc cgcgtgagcc gctctcccgc
     7981 gataagctga tgaaccttgc ccgtggtcgt gaatattccg caatggaacg ctccatcgac
     8041 gtgcagattt cgcgtctgcg ccgcatggta gaagaagatc cagcgcatcc gcgttacatt
     8101 cagaccgttt ggggcctggg ctacgtcttt gtaccggacg gctctaaagc atgaggcgat
     8161 tgcgcttctc gccacgaagt tcatttgccc gtacgttatt gctcatcgtc accttgctgt
     8221 tcgccagcct ggtgacgact tatctggtgg tgctgaactt cgcgattttg ccgagcctcc
     8281 agcagtttaa taaagtcctc gcgtacgaag tgcgtatgtt gatgaccgac aaactgcaac
     8341 tggaggacgg cacgcagttg gttgtgcctc ccgctttccg tcgggagatc taccgtgagc
     8401 tggggatctc tctctactcc aacgaggctg ccgaagaggc aggtctgcgt tgggcgcaac
     8461 actatgaatt cttaagccat cagatggcac agcaactggg cggcccgacg gaagtgcgcg
     8521 ttgaggtcaa caaaagttcg cctgtcgtct ggctgaaaac ctggctgtcg cccaatatct
     8581 gggtacgcgt gccgctgacc gaaattcatc agggcgattt ctctccgctg ttccgctata
     8641 cgctggcgat tatgctattg gcgataggcg gggcgtggct gtttattcgt atccagaacc
     8701 gaccgttggt cgatctcgaa cacgcagcct tgcaggttgg taaagggatt attccgccgc
     8761 cgctgcgtga atatggcgct tcggaggtgc gttccgttac ccgtgccttt aaccatatgg
     8821 cggctggtgt taagcaactg gcggatgacc gcacgctgct gatggcgggg gtaagtcacg
     8881 acttgcgcac gccgctgacg cgtattcgcc tggcgactga gatgatgagc gagcaggatg
     8941 gctatctggc agaatcgatc aataaagata tcgaagagtg caacgccatc attgagcagt
     9001 ttatcgacta cctgcgcacc gggcaggaga tgccgatgga aatggcggat cttaatgcag
     9061 tgctcggtga ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa accgcgcttt
     9121 accccggcag cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg gtggcgaata
     9181 tggtggtcaa cgccgcccgt tatggcaatg gctggatcaa agtcagcagc ggaacggagc
     9241 cgaatcgcgc ctggttccag gtggaagatg acggtccggg aattgcgccg gaacaacgta
     9301 agcacctgtt ccagccgttt gtccgcggcg acagtgcgcg caccattagc ggcacgggat
     9361 tagggctggc aattgtgcag cgtatcgtgg ataaccataa cgggatgctg gagcttggca
     9421 ccagcgagcg gggcgggctt tccattcgcg cctggctgcc agtgccggta acgcgggcgc
     9481 agggcacgac aaaagaaggg taaataaacg ggaggcgaag gtgcctcccg ttttgctttc
     9541 tataagatac tggatagata ttctccagct tcaaatcatt acagtttcgg accagccgct
     9601 accagcgcgg cacccgcagg ggtgtcggtg tatttatcga agttgtcgat aaacagtttc
     9661 gccagggttt cggctttttc ctgccactgt tccggagaag cgtaggtgtt acgcggatcg
     9721 agaatcttcg tgtctacgcc cggcagttcg gttgggatcg ccaggttaaa catcggcaga
     9781 gtgaaggttt ctgcattatc cagcgaaccg ttgaggatgg cgtcgataat ggcgcgggta
     9841 tctttaatcg agatacgttt gccagtgccg ttccagccag tgttaaccag ataagcctgc
     9901 gcgcccgccg cctgcatacg tttcaccagc acttctgcgt actgagtcgg gtgcagcgac
     9961 aggaacgccg cgccgaagca agcggagaag gttggcgtcg gttcggtgat gccacgctca
    10021 gtaccggcca gtttggcggt gaagccagag aggaagtgat actgggtttg atcggcagtc
    10081 aggcgagaaa ccggcggcaa cacgccgaaa gcatcagcag tcaggaagat aaccttagtc
    10141 gcgtggcccg ctttggaaac cggcttaacg atgttatcga tgtgatagat tggataagaa
    10201 acgcgggtgt tctcggtttt tgaaccatca tcaaagtcga tagtgccatc ttcacgcacg
    10261 gtgacgtttt ccagcagcgc atcacgacgg atagcgttgt agatttcagg ttccgcttct
    10321 ttcgacagct tgatagtttt tgcgtagcag ccgccttcga aattaaacac gccgtcatcg
    10381 tcccagccgt gttcgtcatc gccaatcagg cggcgtttcg ggtcggtgga aagggtggtt
    10441 ttaccggtgc cggaaaggcc gaagaacacc gcaacatcgc ctttctcgcc aacgttggcg
    10501 gagcagtgca tagaagcgat acctttcagc ggcagcaggt agttcatcat cgagaacatc
    10561 cctttcttca tttcgccgcc gtaccaggtg ccgccaatca gctgcatgcg ctcggtcagg
    10621 ttaaacgcca cgaagttttc ggagttcaga ccctgttctt tccactgcgg gttagtgcac
    10681 ttcgcgccgt tcataacgat aaagtctggt ttgaaacctg ccagctcttc atcgctcggg
    10741 cgaataaaca tgtttttgac aaaatgcgcc tgccaggcca cttcggtgat gaaacggacg
    10801 gaaagacgag tatccgggtt cgcaccacag aaagcgtcga caacgaacag acgtttgccg
    10861 gaaagctgcc tggtcaccag gcctttcaga tgctgccagg tttccggaga gagaggtttg
    10921 ttgtcgttct tacctttgcc tttgtctgcc caccagaaag tatcgcgagt ggtatcgtca
    10981 cggacgatat acttatcttt tggtgaacga ccggtgaaga tcccggtatc gacggcaacg
    11041 gcacccagat tagttaacac cccgcgctca taacctgtca ggctcggatc gagctcttcc
    11101 tgatacagca ggtcgtagct tgggttgtaa acgatatcat gtacgtcact gataccataa
    11161 gcctcgagtt cttgcggggg caaaccattg ttaacgcgca tttcactgct ccttagccaa
    11221 tatgtattgc ctgaatagta aagtcttttt gggggtgtta accgcgacaa ggctcataga
    11281 tttacgtatc tggagaaatt catatttgac aaaaaactcc gtgattcctg tcacgaaacg
    11341 gttgctatta tcgcagaaat agcattttag gtgtggaaaa tgttctcaaa aggttaaatc
    11401 ttggtttata tggcgggaat attgtgagtg gaatcgcatt cctgaatgaa attgatgcaa
    11461 attcaaccgg taaagtatcg attcaccacg ctaacttttt ttgattattt tctgaaaaaa
    11521 gcaaaatact actgcccgga tgcgataatt aacccacctt ttgattctgg ataaccaaca
    11581 tggataacgt tgaactttca ccggcaacac gctgggggat gattgctacc ggattacttc
    11641 aggggctggt ttgctacctg cttatcgcct ggcttgccgg aaaaaatcac agttggattg
    11701 tttatggcgt gcctgcgacc gtcgcgtttt catccgtttt actcttctcc gtgatctctt
    11761 ttaaacagaa gcgcctttgg ggatggctgg cgctggtgtt tattgccacg acagggatga
    11821 gcggttggct gaagtggcaa actgatggca tgaacccctg gagagctgaa aaggcgattt
    11881 gggattttgg ctgctatctg ctgttgatgg caatgttgtt gctaccgtgg atacaacaaa
    11941 gccttcgtat ccgcaatggc agtagccgct atagctactt ttaccagtca gtatggcata
    12001 acgtacttat attactggtg atttttctct ccaatggcct gacgtggctg gtgcttttac
    12061 tgtggggtga gttgtttaaa cttgttggca tcacgttttt caatacgctc ttttttgcaa
    12121 ccgactggtt tatgtatctt acatttggtc tggttaccgc actggcggtg atcctcgcgc
    12181 gtacacagtc acgtttaatc gactctattc aaaagttgtt cacgctaatc gccacggggt
    12241 tgctgccgtt agtatcattg ctaaccccga tgtttatcat caccctgccg tttacgggcc
    12301 tgagcgcgat ttctcgccac atctccgccg ccgggttgct gttaacgctg gcctttttgc
    12361 aattgatctt aatggctatt gtccgcgatc cgcaaaaagc gtcacttccc tggacggggc
    12421 cgttgcgttg cctgattaaa accgctttgc tggttgctcc gctgtatgtg ttcgtcgcca
    12481 cctgggggtt atggctgcgg gtcgctcagt acggctggac tgtcgaccgc ttgcagggcg
    12541 cgctggcgga gctggtgtta ctggtgtggt cgctggggta ttttgtcagt atcgtctggc
    12601 gtaatgggca aaatcccctt gttcttcagg gcaaagtgaa ccttgcggtt tcgttattgg
    12661 tgttggtgat actggtgctt cttaattcgc cggtgctgga cagtatgcgt attagcgtga
    12721 acagtcatat ggcgcgttat cagagcggca aaaacacgcc agaccaggta actatctaca
    12781 tgctcgaaca gagcggtcgc tatggacgtg cggcgcttga gtcgctgaaa agcgatgccg
    12841 ggtttatgaa agacccgaaa cgcgcgcggg atctgctgat ggcgttagat ggagagcaac
    12901 atcttcagga acaggtatcg gaaaaagtat tcgccgaaaa tgtgttaatt gcccctggtt
    12961 ctgttaaacc ggatgcgaca ttctggtcgg ccttaatcca ggatcgctat aacgtgatga
    13021 cctgtattga aaaagacgcc tgcgtactgg tcgagcaaga tctgaatagt gatggtcagg
    13081 cggagcggat cctgtttgct tttaatgatg acagagtcat tgtctatggc tttgactcag
    13141 acagaaaaga atgggacgcg cttgatatga gtttacttcc gaacgaaata acgaaagaaa
    13201 aattactcac agctgcgaag gatggaaaac tggggacgag gccgaaagcg tggcgcgatc
    13261 tgacggtaga tggtgaaacg ctggaaataa acttaagtaa gtgacaacgc ttttatcgga
    13321 tacgacgctg tatctgacca aaataatttg cgatttattt cataggcgct ggtaagcgca
    13381 gcacatcagg ccattaagtc gtaaaatccg gcagagccct ctctgccgga catactcatt
    13441 aatgaactta cggatctgcc ggagacgcgt tgttgcggat ttcagcaata tccatcgcat
    13501 tgaacagata gtggttaccg cagtaatcac aatgcatgtc aatttcgcca tcttccgcca
    13561 ggatgctatc aacttcttca tcaggcagcg ttttcagcgc atcggcgcaa cgttcacgcg
    13621 agcaggtgca tttgaactcc acatcctgcg gatcgtaaac cgtcacctct tcttcgtgat
    13681 acaaacgcca caacacttcg tttgccggta aggtcagcag ttcttcggtt ttgatggttt
    13741 cggttagcgt cgccaggtgg tcaaagtcgt cctgttgggt attttgcgca ggcattacct
    13801 gcaacaacat accgcctgca gccggtttgc cgtctacgtc gccggtgcga ataaacaggc
    13861 gcgtcggcag ctgttcagaa cgcataaagt aatcttctag gcaggccgcc agggtatcac
    13921 cttccagacc aactacgccc tgatagcgtt cgccttcgct cggggtaatg gtgatcacca
    13981 cgtaaccatt gccgaccagc gttttcaggt cggcattttc tggaatttcg ccctgcacgc
    14041 gcgccacacc gcgcatttgc tggttattgt taccgttaat aaccgccaga ttcatcggac
    14101 cgtcgccctg cagctgtacg gtgatatcac catcaaactt cagcgtagcg gttaacaggc
    14161 tggtcgcaac cagcagttct gccagcacgt ttttaacggg ctgcggataa tcgtggttct
    14221 caaggatctg ttgcagggtt tccgaaacgg ttaccagttc gccgcgaacg gcaaaatttt
    14281 caaacagata gcgatgtaat tggtcatgtt gcggcataat catctctctt gcaggtgaca
    14341 gttattcact gtcgccgtgt ttaaatcgta acaggtcgcg gcgctctttt ttgtccgggc
    14401 gtcggtccgg gtgcggcatg gttaaggcat taagtttacg tgccatcgcc attttttcgc
    14461 gtttctctac actttccgca gtctcttcat acagcaaggc tgcctcgctg gcggggcgac
    14521 gctgttcagt aatcgccttt acaatcaccg tgcgttcgtc atttccctgg cgcagagtga
    14581 gcgtggcatt cagctcgacg attttgctcg gcttgctgcg ctgcccgttg taatgcacct
    14641 taccgccttc aatcatttca cgggccagcg cgcgggtttt ataaaaacgg gcagcccata
    14701 gccatttatc cagtcgaacc tcaacagcag gtttctcttt catggcgtct ccttcacatt
    14761 agcgagggga tcaggcggcg gtagtcattc agtgacggat ggcgttgata ctgtttctcg
    14821 gcaatcccgg aatcaggatt agtcacgccg aggcagtaac gaataccaaa ttgcgcggca
    14881 gcatcgagaa tcgcttcgct gtcatcaata aacagcgttc tttcagcttt cagacccgta
    14941 gcttcggcca ccgcatgcca taaccgctga tcctctttcg gataaccaaa tgtgtgggtg
    15001 gaaagtaata aatcaaggtg tgcgtccaga cccgtatgct caagttttac cgccaggttg
    15061 tgcggatgag cattggtgag caaaattcgc tgcttaccgc tggctttcag tgcctcaaga
    15121 aacggaatgg tatcttcacg cagtacggcg cgcggtccca tctcggtggt catcgcacag
    15181 atatccagac ccagttgctc actccagtaa tcaagacagt accagtttag cgtatgctgt
    15241 acgtcgtgat attgctggcg catatattcc atcgcttcct gtggcgtaac cccgtttttc
    15301 gcgccccatg tttcaggcac cagcttttgc cagaaatagt tatcgaaggc gaggtcgagc
    15361 aacgtgccgt ccatatccag cagaacggta tctacgtcct gccaggcaat gttgatatgc
    15421 atgagggaaa tctccagagt gaagcaattt gcgcgacagg gtagcataac ctgccgcgca
    15481 aacgtgttat tcgataaggc tttctgaagg ggtgatcagt tgcgggttca ggcagctttc
    15541 ataataggcc tgaatctcca tcatgcgagt gcgatgacgc tggtaacgac gccaggcctg
    15601 tacgccattg taaatggcgc tacccagcat cgtcagtagc agcaatgtgg tgctgagata
    15661 gcgccacagg ccggaacgat ccgggatcgg atgaaggcca atatgctgcg taccattggc
    15721 gtcagtgaag atttttgtga cgataccttc ggcgttaaac ggcgtatgca tcagcatttg
    15781 tgccagtttc tggaaagcgt tccactgttc ttgcggcggg tagtcgtaaa gtgatgccga
    15841 aggccagggc tgatcaacaa aatcgctgcc ttcgtcgctg acaatcagga atccgccggg
    15901 ggccggactg tttagtgatt gtgccgctcg cgccgtttca tgcgtgataa acggcgcggt
    15961 ggaggttgcc accaggttat ccagcgattc cgcactcacc gggcgtaata acacattcac
    16021 gccatcgagc ttcccggcgt tggcgcgttt taccagcgcg tcccagtctt tactgttgcc
    16081 gagattgacc agcgcatttt tcagtcgcac acagtcatct ttggcagaac ataaatccgc
    16141 tgtcttcagt acaatgtcgc caaaatcatc aagcaatacc atgccggatt tttgaatcgc
    16201 tgaacgcaat gaggcactga cgcgagattc atcttccggt ttagggtgca gctggcgatt
    16261 aactgcttca gtcaatgccg tcgctttgtt gaccagttca gattctggta atggtaatga
    16321 gcgggcgtca ttccagatga tctgcgagca gtcaaacggt aaaaaaggtg aattggtttt
    16381 cgcgctccag gttccggaag tgcgaatatt acacattccc gtaccgctaa tacgcaatgt
    16441 atcgcctacc cgcacgccag cgtcagccag ttgttttacg ctggtggctt caatggtctg
    16501 cgcgcctttc atccatgaga gagtgaattt cagcggcata tccagcggga tccagaataa
    16561 cagcataaac agcaccagca gcgagcccgc agcgataatc gtactgcgca gccagtgctg
    16621 taacggaaag ttttttactt catcatgcag cgaaagatat cgcccctgac gcactacgtg
    16681 gcggtcgaga tagatatcaa tatcggtttg ttgaccgaga tcctgagcaa tgtatggctg
    16741 ccagtgtgcg ggatagacca ggtcgataat accgagtgaa atattgttga tctgttcctg
    16801 atcgttttcg ccaaacaatc cccaacgacg gggtgtaccg cgcagacaat gaatttctcg
    16861 cagggaggat tttgccgggg gcgcgaatag cccccacaga cctgcgccca gcagcagtaa
    16921 tgcgccgcct gccagccacg gaacaaatac atccggggta atcaggcaga aaaagaacat
    16981 caggaaggag gcgacgatca gcaacgcttc acgcagcccg cgcggacgac tcagcgcata
    17041 ttcttcatgc gtttctttgc gaatattgag tagctcgatt tgctcactct cttccccgcg
    17101 aatcgacgcc tgcgtggacg gaacaggttg caaggcatag ccgcgcgttt cctgcatgta
    17161 ctcctgcagc gtatgaccgt tgagtgaaat aaccaacggc agcgaatcgg tatgaatcag
    17221 ttcaacggta ttctcatcgt tgatgtactg ttcccagaag gggggcaggt ggacttctac
    17281 cgaatcgagg tagtaacgcc atttattcgg atcgtcggta gagatgccgt aacgtgtgat
    17341 agcgtgtgtc agcatcatga cgttgttgct ttcggcattc agcgccagag agatcggtgc
    17401 cgcgctagct cccgttgggc caggcacctg taatacctgc gtcaggctct caagataatt
    17461 ttcaacggcg ctacgttctt caggtgtgag tttacgcgtt tgcgcatccg cgaaggcgtt
    17521 ggtccagggc agctgacgcc gtctggatcg cacttttatc agccatcccg caagtagtga
    17581 gcaggccagc aaagcagcta aaaaaatcac aatggtgctc atgctttccc catcttactt
    17641 agtctgtcag gcgtggtcag tcgcaccgga cacaatacat gattctgcgg ttggctatcg
    17701 gcaagcgtgg agcttaactt gagcatcatt aagcacatca cagtaaccgc tgatcatagc
    17761 aaaccctctg aaggcggaat atcagcaaat tcctggagtt atttcaacct attgactttt
    17821 aatttgattt gaggacgatt atttattaag ttacataaat gtaaataaca tgcaattcac
    17881 attgccgaaa gcgtgtccga tatcgcacaa taaccgctga tttcacagca tagatcccac
    17941 gatgaacaaa tcattacaaa aacccaccat tctgaatgtt gaaactgtag cccgttcccg
    18001 actgtttacc gtcgagagcg tggatctgga gttcagcaat ggcgtgcggc gtgtttatga
    18061 acgaatgcgt ccaaccaacc gggaagcagt gatgattgtg ccgattgtgg atgatcacct
    18121 gatcctgatc cgcgaatacg cagtgggaac tgaatcctac gaattaggtt tttcgaaagg
    18181 attaattgat ccgggtgaaa gcgtctacga agccgctaac cgcgagctaa aagaagaggt
    18241 tggatttggt gcgaacgatc tgactttttt gaagaagctc agcatggcac cgtcttactt
    18301 ttccagcaaa atgaatatcg tggtcgcgca agatctctac ccggaatcgc tggaaggcga
    18361 tgagccagag ccgctaccac aggtgcgctg gccgctggcg catatgatgg atttgctgga
    18421 agaccctgac ttcaacgaag cgcgtaatgt cagcgcgctg ttcctcgtgc gcgaatggtt
    18481 gaaagggcag gggcgagtgt aaatgcgtga agtttgcctg atgcacaacg cttatcaggc
    18541 ttatggggtt cctgcaatat attgattttt cacgattttg ttagccggat aacgcgttca
    18601 cccgcatccg gcataaacaa atgcactttg tcagccaact gaaaaggcgc cgaagcgcct
    18661 ttttaatcag aacaattcct gtgcctcacc attatcgata atggtcgttc ccacctcgtg
    18721 caccgcctgt tgtgtcggct gcgtaccttc gatgaaatac tcttcgcggc tgttgccacc
    18781 attagctaac tgcccggtgc tgcgatcgat attcaccgtc acaatacccg gaggcggcgt
    18841 cagcggctgc tccggcacac cttcaagaac ggctttcata taagcgtccc atgcaggctg
    18901 ggcactcttg gcaccgcctt cgtaacctga gatctgatct ttaatcgctc cggaagccgt
    18961 tgtatgaccg agattacgac ggtgatcatc aaagccaatc cagaccgagg tcacaacgcc
    19021 cggaccgtaa cccgagaacc acgcatcttt cgaactgtta gtggtaccgg ttttcccgcc
    19081 gatatcgcga cgctgcaaat cacgacctgc acgccagccg gtaccctgcc agcctggttc
    19141 accaaagata ttggtgttca aagcactctt aatcaggaat gccagcggag tgttgatgac
    19201 gtgcggtgcg tactcctgcg cgccagtctt cgccactaac gcctgatttg cctgctccag
    19261 ctgcggcatt ggtacagaaa cattctgctg ctcgcgggag atagcgacat cttcaacatc
    19321 gttattttcc agcacgttcg atttctgcgt atcaccgtaa atcactggaa tatcgcattc
    19381 cgggcaggct actttcggtt tcgcttcgaa aatcacgccg ccctgatcgt tttcaatttt
    19441 gctgataaac cacgggtcca ctaggaagcc gccgttcgcc atgaccgcgt agccgcgcgc
    19501 cacctgcatt ggggtgaagg acgctgaacc cagcgccagc gattcggtgt ggacaatgtt
    19561 ttgcgccggg aagccgaagc gttgcagata ttctgcagcg tagtcgacgc ccatcgcccg
    19621 cattgcacgt accatcacca cgtttttcga ctgacccagc ccctgacgta agcgaattgg
    19681 accagcatac tgcggtggtg agttcttcgg ctgccagtca gaaccggcac ctgcatccca
    19741 gcgagaaatt ggcacatcgt tcaacatact tgccagcgtc agacctttat ccatcgccgc
    19801 ggtgtagagg aacggtttga tgttggaacc cacctgacgc agtgcctggg tggcgcggtt
    19861 aaacttgctc tgattgaaat caaagccacc gaccagcgcc ataacggcac cattttgcgg
    19921 attgatcgac accagcgccg agttcacttc cggcacttgt gccagccacc atgcatcgcc
    19981 aacctgacga acccagattt gctgacccgt ttgcagaaca tcggtcactt tacgcggcgt
    20041 cggtccctgc tgagtatccg aacggtaagg acgcgcccag cgaacgcctt ccatactcaa
    20101 tgcgacggtc gacccgtccg ccagcatcgc cgtcgctccc tgaggattgg cgctggtgac
    20161 tgcggcaggc agcagcggac cataggtcgg cagcgccttc agcgtatcgg taatcttgtt
    20221 gttatcccac gccgactcgc ccactttcca cagcacattt gccgggccgc gatagccgtg
    20281 gcgcatgtcg tagtccagca cgttattacg taccgcctgc tgcgcggcct gctgcacttt
    20341 gcgggtgatg gtggtgtaaa tgcgataacc gtcttcatag gcactttcgc cataacggtt
    20401 atacatctcc tggcgcacca tttcgctcag gtacggcgca gagaaagcaa tctccggcgc
    20461 gtgataatta gcgttaatcg cctcagtgcg tgtctgatcg aactgttgct gggtgatata
    20521 cccttcatcc agcatccgcg acagtacgac gttacgccgc gcgacggcac gatccattga
    20581 gtagagcgga ttaaaggtgg aaggcgcttt cggcaacccg gctatcaccg ccatttcgtt
    20641 cagcgtcagt tggtcgaccg ttttaccgaa atagacttgt gccgcagcac cgacaccata
    20701 ggcgcggtaa ccaaggtaaa tcttgttcag ataaagctcg aggatttcgt ctttcgtcag
    20761 caactgttca atgcgaatcg cgaggaagac ttccttaatc ttacgcatca tcgtgcgttc
    20821 tggactgagg aagaaatttc tcgccagctg ctgggtaatg gtactcgccc cttgtgacgc
    20881 gtggccggag aacagcgcca cacttgccgc acggaagatc cccaccgggt caactccgtg
    20941 atgctcgtag aagcggctgt cttccgtcgc gataaaggct ttcaccatct ccggtgggat
    21001 ttgatccaac gtaaccggaa tacgacgttt ctcaccgtat tgagcaatca gctcgccatc
    21061 ggcgctgtaa atctgcatcg gaatttgcag gcgaacatct tttaatgtca ccacatccgg
    21121 cagttgtggc tcgatgtagc ggtataggcc ataaatcgag cctgctccca gcagaatgca
    21181 acagactgca aggatcaaaa aatactttac gaacttcact ggaaatttcc catttagttt
    21241 catttgggca gtttataaac aaacgcgcgg tagtataaag gcaagccaga cgcattgata
    21301 tacccgtcag agtgacgggt gataaggaga tcatcacaat gacatttaag atctggcaaa
    21361 ttggtttgca tttacaacag caagaagcgg cagcggttgc gatcgtgcgg ggcgcaaaag
    21421 aatgcttttt gcaacgctgg tggcggttgc cgctggcgca cgacattatc aaagatgggc
    21481 ggattgttga tgcgcagcag ctggctaaaa cgttgttacc gtggagtcgc gaactgccgc
    21541 agcgtcatca cattatgttg gcgtttcccg ccagtcgcac attacagcgg tcatttccgc
    21601 gcccgtcgat gtcccttggt gagcgggagc aaacggcctg gctgtcaggg acgatggccc
    21661 gcgagctgga tatggatccg gactccctgc gcttcgatta cagcgaagac tcactcagcc
    21721 ccgcttataa cgtgactgcc gcgcaaagca aagagctggc aacgctgctt acgctggcag
    21781 aaaggttgcg tgttcatgtt agtgcgatca ccccggatgc cagtgcatta cagcgattcc
    21841 tgcctttttt accttctcat cagcaatgtc tggcctggcg tgataacgaa cagtggctgt
    21901 gggcgacgcg ctatagctgg gggcgcaaac tggcggtggg gatgactagc gcgaaggagc
    21961 tggcggcagc gttatccgtt gatcccgaca gcgtcgcgat atgtggcgaa ggcggatttg
    22021 acccctggga ggccgtttct gttcgtcagc cgccgctacc gccgcctggt ggagactttg
    22081 ccatcgcgct ggggctggcg cttgggaagg cgtactgatg aacccgccaa ttaatttttt
    22141 gccctggcga cagcaacgcc ggaccgcttt tctgcgtttc tggttgctga tgttcgttgc
    22201 gcctctgctg ctggccgtcg ggataacgct aatactgcgt ctgacaggca gcgccgaagc
    22261 gcgcatagac gcagttttgc ttcaggcgga acaacaactc gcccgcagct tacagataac
    22321 gaagccacgt ttgctggagc aacagcagtt acgcgaacag cgttcgcaac ggcagcgcca
    22381 gcgacaattt acccgcgact ggcaatctgc gctggaagca ctggcggcgc ttttacccga
    22441 gcacgcctgg ctgacaacga taagctggca gcagggaacg ctggagatca aggggctgac
    22501 aacaagcatt accgcgttaa acgcactaga aacgtcactc cgccaggatg cgtcttttca
    22561 tctcaatcag cggggagcca cgcagcagga tgcgcaggga cgctggcaat ttgagtatca
    22621 gttaacaagg aaggttagcg atgaacatgt tctttgactg gtggttcgcc acatcacccc
    22681 gcctccgcca gttttgctgg gcattctggt tgctgatgtt agttacgctc atttttctat
    22741 catcgacaca ccatgaagag cgcgacgcat taattcgact acgggcaagt catcaccagc
    22801 agtgggccgc actgtatcgc ctggtagaca ccactccctt cagcgaggaa aaaacgctgc
    22861 ccttttcgcc actggatttt cagttatccg gcgcgcaact ggtttcctgg catccatccg
    22921 cgcagggagg cgagttggcg ttgaaaacgc tgtgggaagc agtgccgtcg gcatttacac
    22981 ggctggcaga gcgcaacgtc agcgtgagcc gtttttcgtt aagcgtggaa ggtgatgatc
    23041 ttttgttcac gctacaactg gagatgccgc atgagggtta aacgctggtt gttggcaggt
    23101 attgcattgt gccttttaac cggtatgcgt gaccctttta aatcgccgga agatctatgc
    23161 cggattagcg aacttagcca gtggcgctat caggggatgg tagggcgagg cgagcgcatc
    23221 atcggtgtaa taaaagacgg gcaaaagaaa tggcgacggg tgcagcaaaa cgatgtgctg
    23281 gaaaacggct ggacaatttt acagctgacg ccagacgtac taacgctggg taccgggaca
    23341 aactgcgaac cgccacaatg gttgtggcaa cggcaaggag atacaaatga agcaatggat
    23401 agccgcacta ctgttgatgc tgatacccgg cgtacaggcg gcaaagccgc aaaaagtgac
    23461 gctgatggtg gatgacgttc cggtagctca ggtgttgcag gcgctggctg aacaggagaa
    23521 gttgaacctg gtcgtgtcgc cagacgtcag cggtacggtg tcgttacatc taacagatgt
    23581 tccctggaag caggcactac aaactgtagt gaaaagcgcc ggactgataa cgcggcagga
    23641 aggcaacatt ctctcagtgc attccattgc ctggcagaat gacaatatcg cccgccagga
    23701 ggcggagcag gcgcgggcgc aggcaaatct gccgctggaa aatcgcagta taaccctgca
    23761 atacgccgac gcgggagaac tggcgaaagc gggggagaag ctactgagtg ccaaagggag
    23821 tatgaccgtc gataaacgca ccaatcgcct tttgctacga gataacaaaa cggcgttaag
    23881 cgcgcttgaa cagtgggtag cgcaaatgga tctgccggtc gggcaggttg agctgtcggc
    23941 gcatattgtc accattaatg aaaaaagttt gcgtgagtta ggcgtgaaat ggacgctggc
    24001 cgatgcgcaa cacgctggtg gcgttgggca agtcaccacg cttggtagcg acctctccgt
    24061 agcgacggcg acaacgcatg tcggttttaa cattgggcgc atcaacggac gcttgctgga
    24121 tcttgagctt tccgcgctcg aacaaaaaca gcagctggat attatcgcca gtccgcgtct
    24181 gctggcctca catcttcagc ctgccagcat taaacaggag agcgaaattc catatcaggt
    24241 ttccagcggg gaaagtggcg cgacgtcggt ggaatttaaa gaggccgtcc tggggatgga
    24301 ggtcacgccc acggtgttac aaaaaggtcg catccggctg aaattacaca tcagccagaa
    24361 cgttccgggg caggtgctac agcaggccga tggcgaagtg ctggcgattg ataagcagga
    24421 gatcgaaacg caggtcgagg taaaaagcgg agaaacgttg gcgctgggcg gcatttttac
    24481 ccgtaaaaat aaatcgggtc aggatagcgt accgttgctt ggcgacattc cctggttcgg
    24541 gcaattattt cgtcatgacg gaaaagaaga tgaacgacgc gagttagtgg tgtttatcac
    24601 gccacgactg gtttccagtg agtaaacagc cgtaaaagcg gtaatgtttt tatgctgaac
    24661 gtgtttcatc tatttgacgc gcgcaggtat ttagcataca aggagtaccg atttgagagt
    24721 tggtgctctt cgctgcctgc gttccatgat gatgatttat cattcaggcg gcattttgct
    24781 gtctttttta cgctaatctt acccggtgat ttatcgccag agcggtggta gcaaggcagc
    24841 gcgcttgcag cgaccagata tgcagaggga tgggtgattt attcagttgc caaacccgct
    24901 ggagtattga gataattttc agtctgactc tcgcaatatc ttatgaggtt tcagttcatg
    24961 tcctgcggcg ctctctgagc gaagcgggtt tatcattaac gaatagtctt agtagtaccg
    25021 aaaaaatggc agagaaacgc aatatctttc tggttgggcc tatgggtgcc ggaaaaagca
    25081 ctattgggcg ccagttagct caacagctca atatggaatt ttacgattcc gatcaagaga
    25141 ttgagaaacg aaccggagct gatgtgggct gggttttcga tttagaaggc gaagaaggct
    25201 tccgcgatcg cgaagaaaag gtcatcaatg agttgaccga gaaacagggt attgtgctgg
    25261 ctactggcgg cggctctgtg aaatcccgtg aaacgcgtaa ccgtctttcc gctcgtggcg
    25321 ttgtcgttta tcttgaaacg accatcgaaa agcaacttgc acgcacgcag cgtgataaaa
    25381 aacgcccgtt gctgcacgtt gaaacaccgc cgcgtgaagt tctggaagcg ttggccaatg
    25441 aacgcaatcc gctgtatgaa gagattgccg acgtgaccat tcgtactgat gatcaaagcg
    25501 ctaaagtggt tgcaaaccag attattcaca tgctggaaag caactaattc tggctttata
    25561 tacactcgtc tgcgggtaca gtaattaagg tggatgtcgc gttatggaga ggattgtcgt
    25621 tactctcggg gaacgtagtt acccaattac catcgcatct ggtttgttta atgaaccagc
    25681 ttcattctta ccgctgaaat cgggcgagca ggtcatgttg gtcaccagcg aaaccctggc
    25741 tcctctgtat ctcgataagg tccgcggcgt acttgaacag gcgggtgtta acgtcgatag
    25801 cgttatcctc cctgacggcg agcagtataa aagcctagct gtactcgata ccgtctttac
    25861 ggcgttgtta caaaagccgc atggtcgcga tactacgctg gtggcgcttg gcggcggcgt
    25921 agtgggcgat ctgaccggct tcgcggcggc gagttatcag cgcggtgttc gtttcattca
    25981 agtcccgacg acgttactgt cgcaggtcga ttcctccgtt ggcggcaaaa ctgcggtcaa
    26041 ccatcccctc ggtaaaaaca tgattggcgc gttctaccag cctgcttcag tggtggtgga
    26101 tctcgactgt ctgaaaacgc ttcccccgcg tgagttagcg tcggggctgg cagaagtcat
    26161 caaatacggc attattcttg acggtgcgtt ttttaactgg ctggaagaga atctggatgc
    26221 gttgttgcgt ctggacggtc cggcaatggc gtactgtatt cgccgttgtt gtgaactgaa
    26281 ggcagaagtt gtcgccgccg acgagcgcga aaccgggtta cgtgctttac tgaatctggg
    26341 acacaccttt ggtcatgcca ttgaagctga aatggggtat ggcaattggt tacatggtga
    26401 agcggtcgct gcgggtatgg tgatggcggc gcggacgtcg gaacgtctcg ggcagtttag
    26461 ttctgccgaa acgcagcgta ttataaccct gctcacgcgg gctgggttac cggtcaatgg
    26521 gccgcgcgaa atgtccgcgc aggcgtattt accgcatatg ctgcgtgaca agaaagtcct
    26581 tgcgggagag atgcgcttaa ttcttccgtt ggcaattggt aagagtgaag ttcgcagcgg
    26641 cgtttcgcac gagcttgttc ttaacgccat tgccgattgt caatcagcgt aacaacaaga
    26701 aaggtcaggc cgcttatcaa gcggtctatt agcttcaggt taattgcaac gtggtaagca
    26761 ttaacctttt agtggggtgt taaatggatg aattcaaacc agaagacgag ctgaaacccg
    26821 atcccagcga tcgtcgtact ggtcgttctc gtcaatcttc tgaacgttct gagcgtactg
    26881 aacgtggcga accgcagatc aattttgatg atattgaact tgatgacact gacgatcgcc
    26941 gtccgactcg tgcgcaaaaa gagcgcaatg aggaaccgga aatcgaagaa gaaattgacg
    27001 aatccgaaga tgaaaccgtg gatgaagagc gcgtagagcg tcgtccgcgt aagcgcaaaa
    27061 aagcagccag taaacccgct tctcgtcagt atatgatgat gggcgtcggc attctggttc
    27121 tactgctgtt gatcatcggt atcggttctg cgctaaaagc cccctcgacc acttccagcg
    27181 atcaaaccgc gtctggcgag aagagtattg atcttgctgg caatgcgacc gatcaggcga
    27241 atggcgtgca gccagcgccg ggaaccacgt ctgcggaaaa tactcagcag gatgtttcgc
    27301 tgccaccgat ctcttctacg ccgactcaag ggcaaacccc ggtggcaacg gatggtcaac
    27361 aacgtgttga agtgcagggt gacctgaaca atgcgctgac ccagccacaa aatcagcaac
    27421 agctgaacaa tgtggcggtc aattccacgt tgccgactga acccgcaacg gttgcgcctg
    27481 ttcgcaatgg caatgcatcg cgtgactcgg cgaaaacgca aaccgctgaa cgtccgtcca
    27541 ctacgcgccc agttcgtcag caggcggtga ttgaaccgaa aaaaccgcaa gcaaccgtga
    27601 aaacggagcc gaagccggta gcacagacgc cgaagcgtac tgaaccagct gcccctgtgg
    27661 cgagcacgaa ggcaccggct gcgacttcta cgccagcacc aaaagagacg gcgactacgg
    27721 ctccagtaca gacggcatcc ccggcgcaaa ccacggcaac accagccgct ggagggaaga
    27781 ccgcaggtaa tgttggttcg ttgaaatcgg caccgtccag ccattacact ctgcagctga
    27841 gcagttcctc taactacgac aacctgaacg gttgggcgaa gaaagagaat ctgaaaaact
    27901 acgttgtcta tgaaacgacg cgtaatggtc agccgtggta tgtcctggtt tctggcgtgt
    27961 acgcttcgaa agaagaggcg aaaaaagcgg tatctacatt gccagcagat gttcaggcca
    28021 aaaacccgtg ggcgaaaccg ctgcgtcagg tacaggccga tctgaagtaa tcaaggttat
    28081 ctcccgcaat ggtttatcgt tgcgggagct gcctgaagcg ctggatgctg tcggagcttt
    28141 ctccacagcc ggagaaggtg taattagtta atcggcatga agaaaaatcg cgcttttttg
    28201 aagtgggcag ggggcaagta tcccctgctt gatgatatta aacggcattt gcccaagggc
    28261 gaatgtctgg ttgagccttt tgtaggtgcc gggtcggtgt ttctcaacac cgacttttct
    28321 cgttatatcc ttgccgatat caatagcgac ctgatcagtc tctataacat tgtgaagatg
    28381 cgtactgatg agtacgtaca ggccgcacgc gagctgtttg ttcccgaaac aaattgcgcc
    28441 gaggtttact atcagttccg cgaagagttc aacaaaagcc aggatccgtt ccgtcgggcg
    28501 gtactgtttt tatatttgaa ccgctacggt tacaacggcc tgtgtcgtta caatctgcgc
    28561 ggtgagttta acgtgccgtt tggccgctac aaaaaaccct atttcccgga agcagagttg
    28621 tatcacttcg ctgaaaaagc gcagaatgcc tttttctatt gtgagtctta cgccgatagc
    28681 atggagcgcg cagatgatgc atccgtcgtc tattgcgatc cgccttatgc accgctgtct
    28741 gcgaccgcca actttacggc gtatcacaca aacagtttta cgcttgaaca acaagcgcat
    28801 ctggcggaga tcgccgaagg tctggttgag cgccatattc cagtgctgat ctccaatcac
    28861 gatacgatgt taacgcgtga gtggtatcag cgcgcaaaat tgcatgtcgt caaagttcga
    28921 cgcagtataa gcagcaacgg cggcacacgt aaaaaggtgg acgaactgct ggctttgtac
    28981 aaaccaggag tcgtttcacc cgcgaaaaaa taattctcaa ggagaagcgg atgaaacagt
    29041 atttgattgc cccctcaatt ctgtcggctg attttgcccg cctgggtgaa gataccgcaa
    29101 aagccctggc agctggcgct gatgtcgtgc attttgacgt catggataac cactatgttc
    29161 ccaatctgac gattgggcca atggtgctga aatccttgcg taactacggc attaccgccc
    29221 ctatcgacgt acatctgatg gtgaaacccg tcgatcgcat tgtgcctgat ttcgctgccg
    29281 ctggtgccag catcattacc tttcatccag aagcctccga gcatgttgac cgcacgctgc
    29341 aactgattaa agaaaatggc tgtaaagcgg gtctggtatt taacccggcg acacctctga
    29401 gctatctgga ttacgtgatg gataagctgg atgtgatcct gctgatgtcc gtcaaccctg
    29461 gtttcggcgg tcagtctttc attcctcaaa cactggataa actgcgcgaa gtacgtcgcc
    29521 gtatcgacga gtctggcttt gacattcgtc tggaagtgga cggtggcgtg aaggtgaaca
    29581 acattggcga aatcgctgcg gcgggcgcgg atatgttcgt cgccggttcg gcaatcttcg
    29641 accagccaga ctataaaaaa gtcattgatg aaatgcgcag tgaactggca aaggtaagtc
    29701 atgaataagt ttgaagatat tcgcggcgtc gcttttgatc ttgatggtac gctggtcgac
    29761 agtgctcctg gtcttgctgc tgcggtagat atggcgctgt atgcgctgga gttgcccgtc
    29821 gcaggtgaag aacgcgttat tacctggatt ggtaacggcg cagatgttct gatggagcgc
    29881 gcattgacct gggcgcgtca ggaacgtgcg actctgcgta aaacaatggg gaaaccgccc
    29941 gttgatgacg acattccggc agaagaacag gtacgtattc tgcgtaaact gttcgatcgc
    30001 tactatggcg aggttgccga agaggggacg tttttgttcc cgcacgttgc cgatacgttg
    30061 ggcgcgttgc aggctaaagg cctgccgcta ggcttggtca ccaacaaacc gacgccgttc
    30121 gtcgcgccgc tgctcgaagc cttagatatt gccaaatact ttagcgtggt tatcggcggc
    30181 gatgatgtgc aaaacaaaaa accgcatccg gacccgctgt tactggtggc tgagcggatg
    30241 ggaattgccc cacaacagat gctgtttgtc ggcgactcac ggaatgatat tcaggcggca
    30301 aaagcggcag gttgcccatc agttggctta acctacggat acaactacgg cgaggctatc
    30361 gatctcagcc agcctgatgt aatttatcag tctataaatg accttctgcc cgcattaggg
    30421 cttccgcata gcgaaaatca ggaatcgaaa aatgactaag cccatcgttt ttagtggcgc
    30481 acagccctca ggtgaattga ccattggtaa ctacatgggt gcgctgcgtc agtgggtaaa
    30541 catgcaggat gactaccatt gcgtttactg tatcgttgac caacacgcga tcaccgtgcg
    30601 ccaggatgca cagaagctgc gtaaagcgac gttggatacg ctggccttgt atctggcttg
    30661 tggtatcgat cctgagaaaa gcaccatttt tgttcagtcc cacgtaccag aacatgcgca
    30721 gttaggctgg gcactgaact gctataccta cttcggcgaa ctgagtcgca tgacgcagtt
    30781 taaagataaa tctgcgcgtt atgccgagaa catcaacgct ggtctgtttg gctatccggt
    30841 gctgatggct gcggacatcc tgctgtatca aactaatctg gtaccggtgg gtgaagacca
    30901 gaaacagcac ctggaactga gtcgcgatat cgcccagcgt ttcaacgcgc tgtatggcga
    30961 gatctttaag gtgccggagc cgtttattcc gaaatctggc gcgcgcgtaa tgtcgctgct
    31021 ggagccgacc aagaagatgt ccaagtctga cgataaccgc aataacgtta tcggcctgct
    31081 ggaagatccg aaatcggtag tgaagaaaat caaacgcgcg gtcactgact ccgacgagcc
    31141 gccggtagtt cgctacgatg tgcagaacaa agcgggcgtt tccaacctgc tggatatcct
    31201 ttctgcggta acgggccaga gcatcccaga actggaaaaa cagttcgaag gcaagatgta
    31261 tggtcatctg aaaggtgaag tggctgatgc cgtttccggt atgctgactg aattgcagga
    31321 acgctatcac cgtttccgca acgatgaagc cttcctgcaa caggtgatga aagacggcgc
    31381 agaaaaagcc agtgtacacg cttcccgtac gctgaaagcg gtgtacgaag cgattggttt
    31441 tgtggcgaag ccgtaatatc aaaacgctcg gacggtcccc tcgccccttt ggggagaggg
    31501 ttagggtgag gggagcaggc aagcgccagt gggaattacc ctttgctcgc attgtcgaat
    31561 aaatcacttc gtcatcactc tgaaaccggg gaaacccggt ttttttatcc tcttttcgca
    31621 acaacccgaa aaaatgtgaa gcgcctcgcc gtttccacat ctcgtcgtcg ccacttatct
    31681 tcattttaat gaagatataa atattcattt tattgaaaat atattatgcg tcgaacgttt
    31741 atcaaaaaag aaggcgtcgt catcacgaca ctggcccgtt atttgttggg tgaaaaatgc
    31801 ggtaatcgac tgaaaaccat agatgagctg gcaactgaat gccgttcatc ggttggcctg
    31861 acgcaggccg cgttgaaaac gctggaatca agcggagcga tacggattga acgccgtggg
    31921 cgcaatggca gttatcttgt cgagatggat aacaaagcat tgctgagtca tgtggatatc
    31981 aacaacgtgg tatgtgcgat gcctttgccc tatacccgct tgtacgaagg tttagcgagc
    32041 ggattgaaag cccagtttga tggcattcct ttttactatg cgcatatgcg cggcgcggat
    32101 attcgcgtgg agtgtttact taacggtgtc tatgacatgg cagtagtttc ccgcctggcg
    32161 gcggaaagtt atctcagcca aaacaatttg tgcatcgccc tcgagcttgg cccgcatacg
    32221 tatgttggtg aacatcaact gatatgccgc aaaggtgagt ctggaaatgt taagcgagta
    32281 ggtctcgata gtcgttcggc ggatcagaaa atcatgaccg atgtcttttt tggcgatagt
    32341 gatgtggaac gagtcgatct ctcttatcac gagagtttac aacgcattgt taaaggcgat
    32401 gtcgatgcgg tgatctggaa cgtggtggcg gaaaacgaac tgaccatgct gggattagag
    32461 gcgacgccgc taacagacga tccgcgattt ttacaggcca ccgaagccgt ggtcctgacg
    32521 cgagtcgatg attacccaat gcaacaattg ctgcgtgctg ttgtagataa acacgccctg
    32581 cttgcccatc aacaacgggt ggtgagtggg gaacaggaac ccagttatta aacgaaaggc
    32641 atctgatatg gaaaccagac tcaacctgct ttgcgaggca ggcgttattg ataaggacgt
    32701 ctgcaaaggc atgatgcagg tcgtcaacgt actggaaaaa gagtgccatc tgccggtgcg
    32761 cagtgagcaa ggaacgatgg cgatgacaca tatggcgagt gcactgatgc gcagtcgccg
    32821 tggtgaagaa atagagccgc tggataacga gttgctggcg gaactggcgc aatccagcca
    32881 ctggcaagcc gttgtgcaat tgcatcagga gttgttgaag gaattcgctc tggaagttaa
    32941 cccgtgtgaa gaaggctatt ttctggccaa tctttatgga ttatggatgg ctgctaacga
    33001 agaggtttga agctcccgac aaatccacgt cgcacaacct aaatattcaa aaaaatgaat
    33061 atttaattca aggaacagaa acgaggcaac aagatgtttg tagaagcatt gaaacgccag
    33121 aacccggcgc tgatttccgc cgcactaagt ttgtggcagc agggcaaaat cgccccggac
    33181 agctgggtga tcgacgtgga tcagatactg gaaaacggta agcggctcat tgagacggcg
    33241 cggctttacg gcattgaact gtatctgatg accaagcaat ttggtcgtaa tccgtggctg
    33301 gcggaaaaat tgctggcatt aggctacagc ggcattgtgg cggtggatta caaagaggcg
    33361 cgagtcatgc gccgcgctgg tttgccagtg gcgcatcagg ggcatctggt acaaatccct
    33421 tgtcatcagg ttgctgacgc cgttgaacag ggcaccgaca tcatcaccgt gtttactctc
    33481 gacaaagcgc gggaagtttc tgcggcagcg gttaaggccg gacgggtcca gcctgtgctg
    33541 ctcaaagttt atagcgacga tgattttctt tatccggggc aggagagcgg ttttgtacag
    33601 cattcgctgc acgaggtgat tgctgaaatt aagaaactgc cggggctgca tttagccgga
    33661 ctaacccatt tcccttgcct gctttgggat gaggctgctg ggaaagtttt gccaacaccg
    33721 aatcttcaca cgctggtaca agcacgggat caactggcga aatctggtat tgcgattgag
    33781 caactgaacg taccttcagc gaccagctgc acttcgctgc cattgctggc ggaatacggt
    33841 gcgactcatg ccgaacccgg tcatgcgctg accggtacta ttccggcaaa ccagcagggc
    33901 gatcagcctg aacgtatcgc gatgctctgg ttaagtgaaa tctcccatca tttccgtggc
    33961 gacagctact gctacggcgg cggttactac cgtcgtggtc atgcgcaaca tgcgctggtg
    34021 tttacgccag aaaatcaaaa gattactgaa accaatctca aaactgtgga tgacagcagt
    34081 atcgactaca ccctgccgct ggcaggcgag tatccggtga gcagtgcagt ggtgctctgt
    34141 tttcgcacgc agatttttgt cacccgtagc gatgtggtgc tggtgtccgg tattcagcgc
    34201 ggcgaaccag aaatagttgg tcgttatgac agcctcggca accctctgga ggcataaatg
    34261 gcgcgatttg tggtgttagt gattgatagc tttggcgtag gggcaatgaa agatgtcacg
    34321 ctggtgcgcc cgcaagatgc cggggcgaat acctgtggtc acatcctgag ccagttgccg
    34381 catttgcagc taccaacgct ggagaagctg gggctaatca acacattggg ttatgcgcca
    34441 ggcgatatgc agccgtcaga ttccgcaacc tggggcgtgg cagagctgca acatgaaggt
    34501 ggcgatacct ttatggggca tcaggaaatt ttaggcacgc gcccgttacc gccgctgcgg
    34561 atgccttttc gcgatgtgat tgaccgtgtt gagcaggcat tagtttccgc tggctggcag
    34621 gtggagcgcc gtggcgatga tctgcaattt ctgtcggtca atcaggcggt tgcgattggc
    34681 gataatctcg aggcggattt aggccaagtc tataacatta ccgccaatct ctctgtgatc
    34741 tcttttgacg acgcaatcaa aattggtcgt atcgtgcgtg accaggtaca ggtcggtcgg
    34801 gtcattacat ttggcggcct gttaaccaac agtcagcgta ttctcgatgc cgcagaaagc
    34861 aaagaagggc gctttattgg tattaatgcg ccgcgttctg gcgcttatga caacggcttc
    34921 caggtcgtgc atatgggcta cggtgttgat gaaaaagtgc aggtgccgca aaaactgtat
    34981 gaagcgggcg tgccaaccgt gctggtgggt aaggtggcag atatcgtcag caatccttat
    35041 ggtgtgagct ggcaaaacct ggtggatagc cagcggatta tggatatcac cctcgacgaa
    35101 tttaacaccc atccgacggc gtttatttgc atcaatatcc aggaaaccga cctcgctggt
    35161 catgcagaag acgtcgcacg ttatgccgaa cgtttgcagg tcgttgaccg taaccttgcc
    35221 cggcttgttg aggcgatgca gccagatgat tgcctggtcg tgatggcgga tcacggtaac
    35281 gatccgacca ttggtcacag ccaccatacc cgtgaagtgg tgccagtgct ggtttatcag
    35341 caagggctgg tcgccacgca gctcggtgtg cgcaccacgc tttctgatgt gggagctacc
    35401 gtgtgtgaat ttttccgtgc gccaccgcca caaaatggtc gctcttttct ttcctccctc
    35461 cggtttgcag gagacaccct atgagttttg atccgacggg ttacaccctg gcccatgagc
    35521 atctgcatat tgatctctcc ggctttaaaa acaacgtgga ctgccgcctt gatcagtatg
    35581 cgttcatttg ccaggagatg aacgacctga tgacccgggg tgtacgtaat gtgattgaga
    35641 tgaccaaccg ttacatgggg cgcaatgcgc aatttatgct cgatgtaatg cgcgaaaccg
    35701 ggatcaacgt ggtggcctgt actggttatt accaggatgc gtttttcccg gaacatgtgg
    35761 tgacccgcag cgtgcaggaa ctggcgcagg agatggtcga tgaaattgaa cagggtatcg
    35821 atggcacgga gctgaaagcc gggatcatcg cggagatcgg ctccagcgaa ggaaagatta
    35881 cgccgctgga agagaaggta tttattgccg ctgcgctggc gcataaccag accggacgcc
    35941 cgatctccac gcatacgtcg ttcagcacca tggggctgga acaactggct ctgttgcaag
    36001 cccacggagt tgatctttcg cgcgtcaccg ttggtcactg cgatctgaaa gacaacctcg
    36061 acaacatttt gaagatgatc gatctcggcg cgtacgtgca gttcgacacc attggtaaga
    36121 acagttacta cccggacgaa aagcgcattg cgatgctcca cgcgctacgt gaccgtgggt
    36181 tgctgaaccg cgtcatgctg tcgatggata ttacgcgccg ctcccattta aaagccaatg
    36241 gtggttatgg ctatgactat ttattgacca cctttattcc gcaattgcgc cagtcaggat
    36301 tcagtcaggc cgatgtggat gtgatgttac gtgaaaatcc ctctcaattt ttccaataag
    36361 gacagactca tgaaaaagat tggcgttgca ggcttacagc gtgagcagat taaaaaaacc
    36421 attgaagcga cggctcctgg ctgttttgaa gttttcattc acaacgacat ggaagcggca
    36481 atgaaggtga aatccgggca actagattat tacattggcg cgtgtaatac cggtgcgggc
    36541 gcggcattgt cgattgccat cgcggtgatt ggctataaca aaagttgcac cattgccaaa
    36601 ccaggcatta aagcgaaaga cgagcatatc gccaaaatga tcgctgaagg gaaagtggcg
    36661 tttggccttt ccgttgagca cgtcgaacac gcgattccga tgttgattaa ccatctgaaa
    36721 taaaaggcac gactatggat ctgtatattc agattatcgt ggtggcgtgc ctgacgggta
    36781 tgacatcgct tctggcgcat cgctcggcgg ctgtttttca tgacggcatc cacccgatcc
    36841 tgccgcaact gattgaaggc tatatgaacc gtcgcgaggc ggggagtatc gcttttggtc
    36901 tgagcattgg ttttgtggcc tcggtgggga tctcttttac cctgaaaacc gggctgctca
    36961 acgcatggtt actctttctt cctaccgata tcctcggcgt actggcgata aacagcctga
    37021 tggcgtttgg tcttggcgct atctggggcg tgttgatcct tacttgcctg ttgccagtta
    37081 accagctgct gaccgcgctg ccggtggatg tattaggtag cctcggggaa ttaagctcgc
    37141 cggtggtttc tgcttttgca ctgttcccgc tggtggcgat tttctaccag tttggctgga
    37201 agcaaagtct ggtcgccgcc gtggttgttc tgatgacccg tgtggtagtc gtgcgctatt
    37261 tcccacatct taaccctgaa tccatcgaaa tctttattgg catggtgatg ctgctgggga
    37321 tcgcgataac tcacgacctg cgtcatcgtg atgaaaatga catcgatgcc agcgggcttt
    37381 cggtgtttga agagcgcacg tcgcggatta tcaaaaactt accttatatc gccatcgtgg
    37441 gagcattgat tgccgccgtt gccagtatga agatttttgc tggtagcgaa gtttcgatct
    37501 tcacactgga gaaagcgtat tccgcaggcg tgacgccgga acaatcgcaa acgctgatta
    37561 atcaggcggc tctggcagaa tttatgcgcg gactggggtt tgtgccgttg attgccacca
    37621 ccgccttagc aacgggtgtg tatgcagttg cgggctttac ctttgtttat gcggtgggct
    37681 atctctcgcc gaatccgatg gttgcagcgg tattaggcgc agtggttatt tcggcggaag
    37741 tcttgctgct tcgttcgatc ggcaaatggc tgggacgcta cccgtcggtg cgtaatgcgt
    37801 cggataacat ccgtaacgcc atgaatatgc tgatggaagt ggcgctgctg gtcggttcga
    37861 ttttcgcagc aattaagatg gcgggttata ccggattctc tatcgcggtt gccatttact
    37921 tcctcaacga atccctgggc cgtccggtac agaaaatggc ggcaccggtc gtggcagtaa
    37981 tgatcaccgg tattctgctg aatgttcttt actggcttgg cctgttcgtt ccggcttaag
    38041 gaaggcacct atgaagacgt ttcctttgca aagcctgact cttgctgaag cgcagcaaaa
    38101 gcagtttgcg ctggtggaca ctatttgccg ccattttcca ggcagcgagt ttcttgctgg
    38161 cggtgattta gggttaactc caagtctgaa tcaaccgcgt attacccaac gggtggagca
    38221 ggtgctggct gatgcatttc acgcacaggc cgcggcgctg gtgcagggcg cggggactgg
    38281 cgcgattcgc gccgggctgg cggctttgct caaaccgggg cagcgtcttc tggtgcatga
    38341 cgcgcctgtt tacccgacga cacgggttat tattgagcag atggggctga cgcttattac
    38401 tgctgatttc aatgacctgt cggcactgaa gcaggtcgtc gacgagcaac aacctgatgc
    38461 cgcgctggtg cagcatacgc gccagcagcc gcaggacagc tatgtgctgg cagatgtgct
    38521 ggcaacgttg cgcgcggcag gtgttccagc gttaaccgat gataactatg cggtgatgaa
    38581 ggtagcgcga atcggctgtg aatgcggcgc gaatgtctcg acattctcct gcttcaagct
    38641 gtttgggccg gggggcgttg gtgcagtggt tggcgatgcc gatgttatta gtcggattcg
    38701 cgccacgctt tactccggcg gtagccagat ccagggctca caggcgctgg aagtcttgcg
    38761 tggtctggtg ttcgcgccag tgatgcatgc ggtgcaggcg ggagtatccg aacggttgct
    38821 ggctttgctt aacggcggcg cggtggcgga agtgaaaagc gccgtcattg ccaatgcgca
    38881 gtcgaaggtg ttgattgtcg agtttcatca gctgattgcc gccagggtgc tggaagaagc
    38941 gcaaaagcgt ggcgcgttgc cttacccggt cggtgcagag tcgaaatatg aaatcccgcc
    39001 gctcttctat cgactttcag gaacgtttcg ccaggcgaat ccacaactcg aacattgtgc
    39061 gattcgcatt aacccgaatc gcagcggtga agagacggtg ctgcggattt tgcgtgagag
    39121 tattgcgagt atttaatgct ggatagcacc gttcggcaat caagctaaat tccgcactga
    39181 acggtcccct cgcccctttg gggagagggt tagggtgagg ggaacgtgcg ggatgtaaaa
    39241 ttcagtgcgc cagcatcgcc ggatgcgatg ctggcgcatc ttatccggcc tacggttaat
    39301 gtcttttatt atcaatcgtt aaggtgatgc gattggcgcg gcagaagagt tcggagacgt
    39361 gcaccggttt gtcattctga tcaaaggcga ttttgctgat gcgaaacaac ggttcgccca
    39421 gttcgcattt cagccatttt gcctgcgggc gggtggcggc aaagatatcg atggtctttt
    39481 tgtcgctgac cactcgcgtg tcaaaacgct cctgaaataa ctgataggtg gagcttcctt
    39541 cgacgtaaat ctcatcaaag tcaggataac gcgacagcgg gatccatgaa ctatcaataa
    39601 acagcggctc tttatcgaga tacatcaccc ggcagagatg gaacacttcg ctgtttccgg
    39661 ggatgttcag cttttcgcaa aacggcgcgg cgctgacccg ttcctgttcg atcactttct
    39721 ctttcgtcga cttgccttgt gagacgccaa aatcggtaaa accactgaca gtaagcaggg
    39781 cgttttcaac tttctggctt tgtacaaagg tgccttttcc ctgccagcgg atcagtacgc
    39841 cgtctgccac taagtcgctg atggctttgc gaatggtaat gcggctgacg ttatattgtg
    39901 tacaaagctc gttttcggta gggatctgtt gcccggcctg gtaaaccccc tgcgcgatat
    39961 catccagcag ccgctggcgg acggtagcgt agaggagttg atgagagtag cggtccgtag
    40021 ctgacataat cgaccctgaa ataaagaatg aattactcgg attatagggt cgttttccgg
    40081 caagtaacag cacgttggcg tgaagatgct cacgccaacg ttatacctac caggcaccgt
    40141 ggtactgaat ggttttcgcc gcgcacgccg ttccctgcgc catcgcctgc ggtaatgtca
    40201 tccccgcaga ccagccgcaa aggaatccgg caatgaacga atctccggca cccatggtgt
    40261 cgataaccgt caccggttca ggagcctgac gccagaactg cgcgccatcc caggcaatgc
    40321 tgccgttttc acccagcgtg acaatcactg ttcctgcgcc acgggcaaca atcgctttca
    40381 tcttcagacg cagcgcttcg tcttcttgcg gtgcggaggc aaaggcaaaa tcgagatgcg
    40441 gcaccagtgt ctgccagagc gggctgtccc acttgtcgga gaagtcgaaa gcggtaagtt
    40501 tgcccgcagc gtgcagctgt gggaatgcgt cttccgcatg tccccagatt gccgcgtgca
    40561 caatgtcata ctgcgccagc caggcgtaat cctcttcact cagggcaaag tcggccatca
    40621 cgccttcggt gtagtcgcca aaaacgcgat cattgtcgtg cagttccacc tgagtttgtg
    40681 cggtaacgcc gtgtttcgta tggacatggc tgatatcgac gcccatgcgg gcgagatcct
    40741 gcttcagctt tgtgccgtag tcatcgtcac ccacccaggt aatgcatccc ggctgtatgc
    40801 cgtagcgagt gcagtacacc gccacattga ccgcattacc gccagaaaac gctttattca
    40861 gttgcgggta gatatcgacg cagttatcgc cgattgtcgc cagggttttc atctctcatc
    40921 ctccggcagc agcgcgcgaa agcgttccag cgcctggcgg gcatagagtc tgggctcgtt
    40981 catatacatc gtcaccagct ccaccgtaca gtaaccctca tagccccgat caataatatc
    41041 gcgcatcagt tcccgcagcg gcattttgcc ttctccagga atgtaatgcg tgtcgctggc
    41101 cccgtcgctg tcgacaatat gcagatgacg taatttatcg cccagtttgt cgaaataact
    41161 catcaccggt tccgcctgga catacggcgc gcaaatgtcg accatgctga acaggcgcgg
    41221 cgaaggcacc agcgccagcg catgaagcac atcattagca ttacacacga cgttcgattc
    41281 atacggcgtt aacggttcga ggatcagatc catgccgatg ttttccgcgt actcacacag
    41341 ttcgcttaag ttctctgcca gccgccccca gataacgtta ggcggcgtga gatagcccgc
    41401 gtgggccgca gaaatcagcg tataacccgc gttcatctct tttgccatat ctatcgccag
    41461 cttgatcatg tccaggcttt cacgacgcat atgttcatcg cccagcatca tgttatacgg
    41521 atagccgttg gtttctggcg tgtaaccgat aatcggcatc tgatacgtct gcgccagcgc
    41581 cttgatttgt ttgatgccgc ccgcttttaa gtccggcgcg aacgcgtgcg ggcgaccgcc
    41641 ccaaatttcg atgccgtcgt aacccagctc gcttgcatca cgaaatgcgt gttcaatagg
    41701 cagccgctgg tggccgcagg taaacatacc tgttttcatc gggtaactcc ttgcgtaaat
    41761 ccggcccgtt gccgggccgg gagagggatt aatattccac cagaccaccg taatagcggc
    41821 gttcatccgg gttgtgatct ttgtaaatag acaggtagta gcagagccac tccattggca
    41881 cgaacatcag gaacggtgcc agccacgggt gcagcccttg cgaaatttcg gcgtaatcga
    41941 tgacgatcac gttgtcagta cgctgtttaa caaagttaat ggcgcgttcg gtggtgtggc
    42001 gactttcatc attgccgagc aggaacagga acggaacgcc cggttcgaca atctccagcg
    42061 ggccatggcg gaactctccg ctctcaatca cgcagccgtg cgtccaggta aattccatca
    42121 gcgttacaat gccttctttg taacccagcg gacgcagcgg acccgcagca acggtataaa
    42181 tcatcggcca ctggctggcc agttcaccaa gctggcggcc tttttcttcc caggtgcgta
    42241 ccagatgacc gagcgcattc ggcaactgct tgagatcgtt tttgatcttg ccgatttccg
    42301 cgttcggcgc gaggcgggtg atcatctcca gcaccacgct gtagcagagc agcaggtgaa
    42361 tttcccagat acagtcggcc tgataatcaa tgctaaattc cgccgccgag gtaatcgggc
    42421 tgtccgcgcg tttggtgaac gccgcagtga gtgcgccgca ggcccggccc agctccagcg
    42481 ctttgattac ctcttccgtt ttaccgtagt cagaaacgcc aatcaccgcg caacgatcgt
    42541 cgaggcgata cggggtgtta tcgcagaact cccagccgga aatggcgtag acctgaagat
    42601 cggaaaagcg atccgccaga tgtttcgccg tttgcgccgc gttgagtggc gagccgcagg
    42661 caacgaaata aatacgatca atgtcgcgct tcaccatctc ttccacgatg gcgtgcacca
    42721 gcggaacgtc atggctgaga actttttcca cttcctgaac catattttcg gtgaccagaa
    42781 agtccacggt gcttttatca atatccaaca ttttattttc ttcagataac attacgacaa
    42841 agcgttgagc tggcggctac gcttcgccca gaaggcgtaa gcaggcagac cagtagcaat
    42901 aacgatgacg gcgcagataa ggccgggaat cggtgcccag acaaaggttg aggcgaccag
    42961 aatgaggctt gacgcaatgg cgagggtggt catcagcccg aaagccggag tacgccacag
    43021 cggtttgtaa tcgtcgcgtt tacgacacca gatgatggag ccgaaggtga gggtattttt
    43081 gaaacacatc accagggtga aataacccag caggctggtg agatcggaaa cgaagatgaa
    43141 gaagatcccc agcgcccctt gcaggatgat ggagacatcc ggcgtgttgt atttcggatg
    43201 cacatggccg aagcatttaa agaacaggtt gtctttcgcc atcgcgtatt ccagccgcgg
    43261 ctggtacatc acgcagctgg aaagcgaacc aagaatgacg atcatcgccg tgatggcaac
    43321 aaagagccca gcggtgctgc cgagtgcggg gatctaggtc agggcgtcgg aaatcggcgt
    43381 ttcagaattg gcgagtttgt cgaagggcat caggccggaa atcaccagcg ccagcagggt
    43441 gtagagcacc agaaccagca gacaggaacc aatcagcgct cgtggcatgg tttttccggg
    43501 gtttttaatt tcgccggtca tataacagat agaggccatg ccggtatacg accaactggt
    43561 ggcagagatc cccgccagca gcgccataaa gctgcccgtt gcgccaatcg cagtggtggt
    43621 aagggcggca aaattctccg ctttaaacca gaagatccca aggccaatga cgatagtgaa
    43681 cgggataatt ttggcgatgg taattagcgt ctgaaacgct gcgccgcctt caacggagcg
    43741 caggtgtagc aacataaagg cgataattaa tccggcggcg ataaatttac cgagcaacgg
    43801 atcgataggc gttaaaaagc caagattgct gacaatcgcc agcgccataa tcgacaatga
    43861 cggcgcatcg ttggcccaga agctggccca gccggagagg aaagccagcg gtcggcttcc
    43921 ggcatttttc agataaacat aatctgcgcc attttccgga taagcggtgg atagttccgc
    43981 atagacgcac atttgcggga tcacaattaa cccgccaatg acaaacgcga gcaccgtaag
    44041 ccacggcgtg cccgctgctt ttgccacttc acccacagat acaaaaatac cggagccgac
    44101 ggttgtcccg acggcgattg caagaacggc ccaaaatccg agcttgcgtt ggagttcttg
    44161 gcttcccata gcgatacctt tttcttgtcc aatacgttgt aggggagagt ttgcagtcat
    44221 ttaaaaggta acatgacaat gcatgatgaa tataacaaca tattatgtta tgtaatgtgg
    44281 aggaagatca caaaatttcg cacaggatcg cgctgtggct aatggatgta gttataaaat
    44341 tgaatttaaa gtgaaaatat ttttacgggc gggggcaaga aggacataga aacaaatacg
    44401 ccctcggaaa atccagaggg cgtcgggcaa ttaaaccggt gttagccgat ttctgtcaga
    44461 gacttactgt gcagtaggac cgcaaccgcc aatgatttta gaaatagaaa ttgccgggtg
    44521 cagcaggtag tcgtaagagc agttcttatc acggttttca atatgaccag tacatgcagt
    44581 taacgtagcc agtactgcac ctaccagtgc aactttaata aatttgttca tttacattcc
    44641 ctatgtatta tatattaatt aatattcatt gattatatga atataagcag actgcttgtt
    44701 aagaacgata caggaatatt taattggcgt cagtgagcga atgacttaat tttgtaagtc
    44761 gctgtaacgg tgaatataac ccattagcag ataatattta ttaaggggtt atgttttatt
    44821 tgctgaatat ataaaaagag tcattaagaa ggccagaatt ctctggcccg ggttaaatta
    44881 atgattggag aaccagttca gtttatcgcg caggccaaca acccgaccaa taataattag
    44941 cgatggactg ttcatttgct gagcaagttc accgagttgc gtgagcgtac cgtcaatcac
    45001 gcgctgcgtg actgccgttc cgttttcgac aattgccacc ggcatttcgc caggcatacc
    45061 gtgttcaatc agcttttgct gaatagtcgc ggcctgattc aaccccatat agaacaccag
    45121 cgtctgtttt tctgccgcca ggttttccca gtccagctcg ccaccggttt ttaagtgtcc
    45181 ggtaattaag cgtacgctct gggcataatc gcgatgcgtg agtggaatac ccgaataggc
    45241 agagcaacca gaagctgcgg taatacccgg aaccaccgag aacggaatac ccgcgttgca
    45301 cagtgtttcc agctcttcgc caccacggcc aaaaataaac ggatcgccac ctttcagccg
    45361 caccacgcgt ttgccttttt gcgcttcccg cagcaggatc tggttaatct cttcctgcgg
    45421 tacacagtgg tatcccgcgc gtttgccgac gaaaacgcga tcagcatcgc ggcgtaccag
    45481 attcataata tcgtcagaaa ccagacggtc gtagaccacc acatctgcct gctgaatttg
    45541 ttgcagtcct ttcagcgtca gcagcccggc atcgcccgga cctgcaccaa ccagcaccac
    45601 ttcaccgcga tggtcgagcg gttcgttgat taactgttcg gtcgtttcag taatggcttt
    45661 ctgatcgttg tttgccagcg actgcgccag gcggtcgtta acgaacaatt tctcccagaa
    45721 acggcgacgc tcactcatcg tggcgaactg ttgtttcact cgcccgcgta attgcccggc
    45781 atatttcgcc acctggccca gatgcaacgg cagcagtgat tcgagttttt cgcgcaacag
    45841 gcgcgccaga accggagagg tgccgccaga ggagaccgct accattagcg gtgagcggtc
    45901 aataatcgac ggcataataa agctggcggc tttcggcgca tcaactacgt tacagaagat
    45961 gcgacgagct tcagcggctt cgctgacgcg ctggttaagc gtgtcatcat ccgtcgctgc
    46021 aatcgccagc cagcaggtgt cgagaaggct ttcatcaaat ggcccttcga cgagggttaa
    46081 catgcctgca tctgcccatg cggtgaactg tggaataaac gctaatgcat tcaccgttaa
    46141 gcgagcgcct gcgtctaaca gcaaccttgc tttgcgttcc gcgacatcac caccgccgac
    46201 aatcagacag tcgcgatcgc gtaattggca aaatataggc aaatgatcca cgtaaaaacc
    46261 ccttagtaat taaccggcag ctgtttcagt ttgattaaat ttgtccgcaa ccggacgatt
    46321 cgctttcggc gtagcatacc aataacccaa tcccatgaat acggcacctg ataaagtatt
    46381 acccagcgtc acccacagca ggttatgacc aatacccgcc agcgtgtagg cttcgctgtg
    46441 gttgccgaac caggagagcg cgaacagcgt catgttagcg atagagtgct cgtagccgga
    46501 cgcgataaat gccagcagac accaccagat agcgataaat ttcgccgccc cttcagtgcg
    46561 cagcgccatc cagattgcta ggcaaaccag ccagttacac aatgcacctt tgaagaacag
    46621 taccattgcc ggtgcagtgg ttttagccag cgcgacggag tgaacgatgc tggtatctac
    46681 cggcagcagg ctaccgccgc cccagctata gagcatggca acgaagacgg aaccgaccag
    46741 gttacccagc caggtttgcg gcaggattgc ccacatttgc ccgtggctga tgctgcccgc
    46801 ttttacccca aaggtgagga acatggtgtg tccggtgaac agttcagaac cggcgataat
    46861 caccagcgtt aaggcgatac caaaggtcgc gcccatcacc agcggacgta cggacgggtc
    46921 gagcaaatta ccgagcgtga aaatcaggat gatcccaaga cccacatatg cgcccgccat
    46981 ggcggagctg acccaaaagc cgagcgggtt atttgccgac aggcgtgcaa tgcgcgcagc
    47041 attagccgca cacttattaa tagtgtctgt aaacatttga ttatccttta aaaaataaaa
    47101 acaaaaaata aaacaaaaag tttaaaaaat gtcctgcaat ataccga
//
LOCUS       DAJVRL010000022        46125 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVRL010000022 DAJVRL010000000
VERSION     DAJVRL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.022"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..131
                     /locus_tag="OQN02_001198"
                     /pseudo
     CDS             1..131
                     /locus_tag="OQN02_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 family protein"
     gene            complement(119..187)
                     /locus_tag="OQN02_001199"
                     /pseudo
     CDS             complement(119..187)
                     /locus_tag="OQN02_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 family protein"
     gene            complement(240..1574)
                     /gene="adeQ"
                     /locus_tag="OQN02_001200"
                     /gene_synonym="yicO"
     CDS             complement(240..1574)
                     /gene="adeQ"
                     /locus_tag="OQN02_001200"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1642070.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1749..3515
                     /gene="adeD"
                     /locus_tag="OQN02_001201"
     CDS             1749..3515
                     /gene="adeD"
                     /locus_tag="OQN02_001201"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1642071.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(3561..4952)
                     /gene="uhpT"
                     /locus_tag="OQN02_001202"
     CDS             complement(3561..4952)
                     /gene="uhpT"
                     /locus_tag="OQN02_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1642072.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(5090..6409)
                     /gene="uhpC"
                     /locus_tag="OQN02_001203"
     CDS             complement(5090..6409)
                     /gene="uhpC"
                     /locus_tag="OQN02_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1642073.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(6419..7921)
                     /gene="uhpB"
                     /locus_tag="OQN02_001204"
     CDS             complement(6419..7921)
                     /gene="uhpB"
                     /locus_tag="OQN02_001204"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1642074.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(7921..8511)
                     /gene="uhpA"
                     /locus_tag="OQN02_001205"
     CDS             complement(7921..8511)
                     /gene="uhpA"
                     /locus_tag="OQN02_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1642075.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(8586..8876)
                     /gene="ilvN"
                     /locus_tag="OQN02_001206"
     CDS             complement(8586..8876)
                     /gene="ilvN"
                     /locus_tag="OQN02_001206"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1642076.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(8880..10568)
                     /gene="ilvB"
                     /locus_tag="OQN02_001207"
     CDS             complement(8880..10568)
                     /gene="ilvB"
                     /locus_tag="OQN02_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1642077.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(10674..10772)
                     /gene="ivbL"
                     /locus_tag="OQN02_001208"
     CDS             complement(10674..10772)
                     /gene="ivbL"
                     /locus_tag="OQN02_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1642078.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            11337..11426
                     /gene="tisB"
                     /locus_tag="OQN02_001209"
     CDS             11337..11426
                     /gene="tisB"
                     /locus_tag="OQN02_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1642079.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            11706..12890
                     /gene="emrD"
                     /locus_tag="OQN02_001210"
     CDS             11706..12890
                     /gene="emrD"
                     /locus_tag="OQN02_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1642080.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(12898..13395)
                     /locus_tag="OQN02_001211"
     CDS             complement(12898..13395)
                     /locus_tag="OQN02_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1642081.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(13392..13754)
                     /locus_tag="OQN02_001212"
     CDS             complement(13392..13754)
                     /locus_tag="OQN02_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1642082.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(13744..14091)
                     /locus_tag="OQN02_001213"
     CDS             complement(13744..14091)
                     /locus_tag="OQN02_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1642083.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            14200..14613
                     /locus_tag="OQN02_001214"
     CDS             14200..14613
                     /locus_tag="OQN02_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642084.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(14660..16102)
                     /locus_tag="OQN02_001215"
                     /pseudo
     CDS             complement(14660..16102)
                     /locus_tag="OQN02_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(16137..17852)
                     /locus_tag="OQN02_001216"
     CDS             complement(16137..17852)
                     /locus_tag="OQN02_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCS1642085.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            18019..18912
                     /locus_tag="OQN02_001217"
     CDS             18019..18912
                     /locus_tag="OQN02_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1642086.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(18909..19723)
                     /locus_tag="OQN02_001218"
                     /pseudo
     CDS             complement(18909..19723)
                     /locus_tag="OQN02_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19723..21340)
                     /locus_tag="OQN02_001219"
                     /pseudo
     CDS             complement(19723..21340)
                     /locus_tag="OQN02_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            21635..22309
                     /locus_tag="OQN02_001220"
     CDS             21635..22309
                     /locus_tag="OQN02_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1642087.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(22348..24009)
                     /locus_tag="OQN02_001221"
     CDS             complement(22348..24009)
                     /locus_tag="OQN02_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1642088.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(24205..24633)
                     /gene="ibpB"
                     /locus_tag="OQN02_001222"
     CDS             complement(24205..24633)
                     /gene="ibpB"
                     /locus_tag="OQN02_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1642089.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(24745..25158)
                     /gene="ibpA"
                     /locus_tag="OQN02_001223"
     CDS             complement(24745..25158)
                     /gene="ibpA"
                     /locus_tag="OQN02_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1642090.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(25152..25224)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            25463..25795
                     /locus_tag="OQN02_001224"
     CDS             25463..25795
                     /locus_tag="OQN02_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1642091.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(25797..27017)
                     /locus_tag="OQN02_001225"
     CDS             complement(25797..27017)
                     /locus_tag="OQN02_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1642092.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            27118..28218
                     /gene="cbrA"
                     /locus_tag="OQN02_001226"
     CDS             27118..28218
                     /gene="cbrA"
                     /locus_tag="OQN02_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCS1642093.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(28179..29516)
                     /locus_tag="OQN02_001227"
     CDS             complement(28179..29516)
                     /locus_tag="OQN02_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1642094.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(29591..30739)
                     /gene="dgoD"
                     /locus_tag="OQN02_001228"
                     /pseudo
     CDS             complement(29591..30739)
                     /gene="dgoD"
                     /locus_tag="OQN02_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(30736..31353)
                     /gene="dgoA"
                     /locus_tag="OQN02_001229"
     CDS             complement(30736..31353)
                     /gene="dgoA"
                     /locus_tag="OQN02_001229"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS1642095.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(31337..32215)
                     /gene="dgoK"
                     /locus_tag="OQN02_001230"
     CDS             complement(31337..32215)
                     /gene="dgoK"
                     /locus_tag="OQN02_001230"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS1642096.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(32212..32901)
                     /gene="dgoR"
                     /locus_tag="OQN02_001231"
     CDS             complement(32212..32901)
                     /gene="dgoR"
                     /locus_tag="OQN02_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS1642097.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            33179..33835
                     /locus_tag="OQN02_001232"
     CDS             33179..33835
                     /locus_tag="OQN02_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642098.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(33881..34693)
                     /gene="yidA"
                     /locus_tag="OQN02_001233"
     CDS             complement(33881..34693)
                     /gene="yidA"
                     /locus_tag="OQN02_001233"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS1642099.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(34807..35205)
                     /locus_tag="OQN02_001234"
     CDS             complement(34807..35205)
                     /locus_tag="OQN02_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS1642100.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(35445..37859)
                     /gene="gyrB"
                     /locus_tag="OQN02_001235"
     CDS             complement(35445..37859)
                     /gene="gyrB"
                     /locus_tag="OQN02_001235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1642101.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(37888..38961)
                     /gene="recF"
                     /locus_tag="OQN02_001236"
     CDS             complement(37888..38961)
                     /gene="recF"
                     /locus_tag="OQN02_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1642102.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(38961..40061)
                     /gene="dnaN"
                     /locus_tag="OQN02_001237"
     CDS             complement(38961..40061)
                     /gene="dnaN"
                     /locus_tag="OQN02_001237"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1642103.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(40066..41469)
                     /gene="dnaA"
                     /locus_tag="OQN02_001238"
     CDS             complement(40066..41469)
                     /gene="dnaA"
                     /locus_tag="OQN02_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1642104.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            41766..41846
                     /locus_tag="OQN02_001239"
     CDS             41766..41846
                     /locus_tag="OQN02_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642105.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            42077..42217
                     /gene="rpmH"
                     /locus_tag="OQN02_001240"
     CDS             42077..42217
                     /gene="rpmH"
                     /locus_tag="OQN02_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1642106.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            42234..42593
                     /gene="rnpA"
                     /locus_tag="OQN02_001241"
     CDS             42234..42593
                     /gene="rnpA"
                     /locus_tag="OQN02_001241"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1642107.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            42557..42814
                     /gene="yidD"
                     /locus_tag="OQN02_001242"
     CDS             42557..42814
                     /gene="yidD"
                     /locus_tag="OQN02_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1642108.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            42817..44463
                     /gene="yidC"
                     /locus_tag="OQN02_001243"
     CDS             42817..44463
                     /gene="yidC"
                     /locus_tag="OQN02_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1642109.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            44569..45933
                     /gene="mnmE"
                     /locus_tag="OQN02_001244"
     CDS             44569..45933
                     /gene="mnmE"
                     /locus_tag="OQN02_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1642110.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11329 a        12482 c        11694 g        10620 t
ORIGIN      
        1 ccagaaaagc gattcgccgt ctcagcgatc ggctgaatat cgatgtcgta cccgtggagt
       61 cgatggtcga tcaaatggga aagtcagccg gtgacgaatt tttacgttat ttgcatcgcc
      121 cggatgtcag cacccgaaaa ccaaccacaa acactaccgc cagcgtggcg agcattatcc
      181 agatcatgag ttctcctgcg taatcagaac tcatgatatc tggaaaccct cgccagttac
      241 taatccacca gaataatctt caatgcgaac agagctgcga ccaccaccac acacaggttc
      301 agatcgcgcc agcgcccggt gcacactttc atgatgcagt acgacataaa gccgagtgca
      361 atcccttcgg tgatcgagaa agtaaagggc atcatcaccg tggtaataaa cgcaggcacc
      421 gattcggtaa aatcatccca gttaacgcgc gccaggctcg aagtcatcag cacgccaaca
      481 aagattaacg ctccagcggt tgcgtaagga ggaaccatcg ccaccagcgg tgagaagaac
      541 ataactaaca ggaacataac gccgaccacg acagccgtta gccccgtgcg accaccgact
      601 gccacaccag aagtactttc aatataggca gtaacagacg aggtaccgat aaatgcgcct
      661 gccaccgaac tgacgctatc aacatacagc gccttattca tattggggaa tttaccgttg
      721 ctatctatta agcccgcttt atcagttaca ccaattaatg ttcctgatga atcaaatagg
      781 ttgatcagca taaaggagaa aatgataccg gcgagttcaa gtgttaacgc gccgctcaaa
      841 tctacttcac caatgacgcc gctaatatca ggcggaatgg aatagacgcc gctaaaatga
      901 acatcaccga aaaataatcc acagcaagac gtcaccacaa tagaaaccag caccgcggca
      961 tgaaaatgac gtgatgacaa cacggttata ataaaaaacc ctaaaatacc taacaacacg
     1021 ccgtgagaac ttaaatcgcc aatcatcacc agcgtgtctt tattggcgac aataacacca
     1081 gtgtttttta atcccattaa ggcaataaat aatccgattc cactggtgat accaatacgt
     1141 aaacttaatg gaatgttgga gatcatccag taccggatac gaaacagcgt gagcaaaaat
     1201 agcccaattg cgccccagaa tatcgcgccc atcccggtct gccaggagat ccccatcgcc
     1261 cccacgacca cgaacgcaaa gaaggcgttc agccccattg ccggagccag cgccacgggt
     1321 aagttagcaa atatccccat cgcaatactg ccgataccgg caatcaaaca ggtggtaaca
     1381 aacaccactt tcgggtccat ttgtgccgcg ccgaggattt gcgggttcac aaaaacaatg
     1441 tacaccatgg ttaaaaaagt ggtcatcccc gcaatcaatt ctgtgcggac ggtggtccca
     1501 tgctgaggta gtttaaataa tcgcgaaagc gtccctgatt cattactcac gtaatcggta
     1561 ttgtcattat tcattttttt gtccattgga ggagatttaa tcccttgctt ttgtaaaaca
     1621 aatgacaaca acggttcagt gataattatc acatttcaat tgcacattaa tagatattct
     1681 ttaataatct cgcgacgttt ctttatgata aataataatc aaattgataa aatcaaaatg
     1741 agaaaaatat gaataattcc attaaccata aatttcatca cattagccgg gctgaatacc
     1801 aggaattgtt agccgtttcc cgtggcgacg ctgttgccga ttatattatt gataatgtct
     1861 ctattctcga cctgatcaat gccggagaaa tttccggccc aattgtgatt aaaggacgtt
     1921 acattgccgg tgttggcgca gaatacgctg atactccggc tttgcagcgg attgatgctc
     1981 gcggcgcaac ggcggtgcca gggtttattg atgctcacct gcatattgaa tccagcatga
     2041 tgacgccggt cacttttgaa accgctaccc tgccgcgcgg cctgacgacc gttatttgcg
     2101 accctcatga aatcgtcaac gtgatgggag aagccggatt cgcctggttt gcccgctgtg
     2161 ccgaacaggc aaggcaaaac cagtacttac aggtcagctc ttgcgtgccc gctctggaag
     2221 gctgcgatgt taacggtgcc agttttaccc ttgaacagat gctcgcctgg cgggaccatc
     2281 cgcaggttac cggccttgca gaaatgatgg actaccctgg cgtaattaac gggcagaatg
     2341 cgctgctcga taaactcgat gcatttcgct acctgacgct ggacggtcac tgtccgggtt
     2401 tgggtggcaa agaacttaac gcctatattg ctgcgggtat tgaaaactgc cacgaaagtt
     2461 atcagctgga agaaggacgc cggaaattac aactcggcat gtcgttgatg atccgcgaag
     2521 ggtccgctgc cagcaatctc aacgcactgg caccgttgat caacgaattt aacagcccgc
     2581 aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa gggcatatcg
     2641 atgccttaat tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg gcatatcgcg
     2701 tcgccagctg gtcgacggca cgccactttg gtctgaatca cctcggctta ctggcacccg
     2761 gcaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa ggtcacggtg cagcaggtac
     2821 tggtgaaagg cgaaccgatt gatgcgcaaa ccttacaggc ggaagagtcg gcgagactgg
     2881 cgctgtctgc cccgccatat ggcaatacca ttgcccgcca gccagtttcc gccagcgact
     2941 ttgccctgca attcacgccc ggaaaacgct atcgggtcat tgacgtcatc cataacgaat
     3001 tgattacgca ctcccgctcc agcgtctaca gcgaaaatgg ttttgatcgc gatgatgtgt
     3061 gctttattgc cgtacttgag cgttacgggc aacggctggc tccggcctgt ggtttgctcg
     3121 gcggctttgg cctgaatgaa ggcgcgctgg ctgcgacagt cagccatgac agccataata
     3181 ttgtgatgat cggtcgcagt gccgaagaga tggcgctggc ggtcaatcag gtgattcagg
     3241 atggcggcgg gctgtgcgtg gtacgtaacg gccaagtaca aagtcatctg ccgttaccca
     3301 ttgccgggct gatgagcacc gacacggcgc agtcgctggc ggaacaaatt gacgccttga
     3361 aagccgccgc ccgtgaatgc ggtccgttac ccgatgagcc gtttattcag atggcgtttc
     3421 tttctctgcc agtgatcccc gcgctaaaac taaccagtca ggggctattt gatggcgaga
     3481 agtttgcctt cactacgctg gaagtcacgg aataataaaa aaagcccggc gtcatgcagg
     3541 gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt ctcgcggcgg
     3601 attttgcgtt cttccattac cgccactatc gccatcaggc agatacaacc aatcgcggcg
     3661 atatccagcg cggcgaaggt gcctgcccag ccggtaaggc cgaataccgg cgtcccatcg
     3721 gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc aaaggtgcct
     3781 ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc agccacacca
     3841 atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc cagatagata
     3901 tattcgttac tggcatgttg atacacaccg agcgtggcga taatcagcgc cagcgcgatg
     3961 caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca cagcagcgta
     4021 ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc tttagagagt
     4081 ttcagttcct ggaacgcgta tacggttgac cactggtcga taccaatacg taccacatag
     4141 aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac atactcaaca
     4201 aagatctgcc acttggtcat atcggtagat tctgtctctt tgtcctcttc gctgatctcc
     4261 tcgccgaaca gttcttcagc tttgccgagg ccataagatt ccggggagtc gctgccgtaa
     4321 cgcaggccga taaaaccgac aatcagcgcg ataatcgacg ggaagataaa catgccgatg
     4381 acatggccat cgaacaggta atttgccccg aacagcgcca cacctgctgc gcctgcaccg
     4441 ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc cgcgtttacg acgcggcgtc
     4501 catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa gccgcttaag
     4561 gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact ggcactgaag
     4621 cccagcatac aaatggcaga gaggataagc atgaacggca ggaattgttt ggtgtttttg
     4681 ccgtcggcgt agtatgaaac caacgtttta cccacgccat aagtgatgga gaaacccagg
     4741 ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg aaatcatatc gttctgcgcg
     4801 atgttaaagt tcttgcgaat caggtacatc gtcaggtagc cgataaagac caccaggtag
     4861 gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag gtccagggtc
     4921 ggcttgcgaa cctggtttag gaaagccagc atgggttact cctgaaatga atacctgcgt
     4981 gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc aagaaaaacc
     5041 tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat cacgcttcac
     5101 gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg gcggcgatag
     5161 agataaccac aaaaaatccg ctccagtgcc aggtatcgag cactttcgcc agcggccaac
     5221 cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc gcccctgccg
     5281 cctctttgtg ggaacactct gccgccgcca taccaattaa catctgcggg ccaaagacaa
     5341 aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat ggcatcagcc
     5401 acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc ggcccacggt
     5461 tgccgttaaa caatttgtcc gagccccaac cggctaccag cgcaccgata aatccgccca
     5521 gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc gtctcggaca
     5581 tatacaaatt gccccagtcg ttgatcgccg cccggaccac atagaccagc acatagcaaa
     5641 acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc tctttacgcg
     5701 tcaaccctgc cccttcttgt tgttgagcaa tttccagcgc gtcgtgccgc cattcaccga
     5761 ccgccggtaa acctaacgcc tgcgggcgat cgcgtagccg ccagcagaga aaaatcccca
     5821 cgactatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa tgcagcgcgc
     5881 tcgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg ttccataatg
     5941 cccaccaacc gccgcgctcg gtacgtgaat accaggccgt taacagacgc gcacacaccg
     6001 gtgaacccca gccctggaaa aaggcgttca gcacccagag cacggcaaac gcccatagcg
     6061 acgtcgagaa gccaaacaga atgttcatga tgcccgtggc gataagccct atccccataa
     6121 aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac acgccgtaag
     6181 taatgtaaaa cagggtcgct aacaggccga tatcgctacg gctgagcacg ccgttagcaa
     6241 ggatttctgg taccgcggcg ttaaaacttt tccgcgtgaa gtaaaacagc gcgtaaccca
     6301 gccagatggt cagcagaata tgccgacgcc agtagcgata acgggcatca atttcatgtt
     6361 tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc atcgaacctt
     6421 agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag gaaatgtgta
     6481 atgtgccgcc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa ccttgttgcc
     6541 cggaacccgg cggcaaaccg ctgccatcgt cttcaatcac cagcatcaac cgttcatcct
     6601 gctgccagcc ttgcagcgta accgcgctgg catcggcatg tttcacaatg ttgttcagcc
     6661 cttcctggca gacacgaaac agcgtcacgc gctggttttc gcttaatgct gattcatcga
     6721 tacgccattc gagatggctg acaatgccgc gcccttccag ctccatttcc cgcatcagtg
     6781 agcggatggc ctgctccagg gtgagatcat ccagctggcg cggacgtaac cgtcctaaca
     6841 aacggcgcac cgcgtcgtaa acgcccagcg acagttgttc gatgagctgc ccgctctgct
     6901 tcacactggc gttatctgcc gccagccgct gaacaatgcc cgcctgagta cgaatagcag
     6961 tgatggtctg accgatatcg tcgtgcagct cacgcgccac gtcgcggcgc acgctctctt
     7021 cggtttctaa caaacgttca gccagatgct gattgcgcgc cagttccttt tgtagcgact
     7081 ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa caaccctgtc agactttgca
     7141 ccagcaacga gagtaataaa tccaccagat gatcgcgcca ggtttgactg gcgatcagcg
     7201 cgatggcgtt catcaacgtc gcaatcagcg ctccttgcca gccatagtgc caggccagcg
     7261 cgataatcgg cagcgccaga cagaatggcg taaagcgcga cagttcgtcc ggcaatccca
     7321 actggagcca gagactgata acaaacagca gcaagtacca gaccagatgc cgcccgcgcc
     7381 agttgatggg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta ttggcgagat
     7441 agtgccagaa caccagacat atcggggcca gcgtcagacc gccagttaaa gtcagcaaca
     7501 gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac aacgccgccg
     7561 ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc acgctgatgg cgatagcgcg
     7621 agatcagcgc tacgggcagt aacgtcagta aactaccgat catcaataac ggaaaatggg
     7681 ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ctccgcaccc agcaatacgg
     7741 gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac ggaaataaca
     7801 gcaccgccat atcagggcgc tcaaccagat gcaggctgat actccatagg caaaaccatg
     7861 cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac aacgtcttca
     7921 tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg ttactgacgc ccagtttttc
     7981 catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt cggcggcaat
     8041 ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac gtttggtcag
     8101 cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat aacagccgcc
     8161 cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt tggaaagaaa
     8221 gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca ggactgtcgt gaacggagag
     8281 cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg agatatcggg
     8341 catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg ccagcgcctc
     8401 gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca gcagctgcgc
     8461 aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca tggtcttgtc
     8521 ctggcgggta aaaaaaaacg cgcttacctt aacgataagc gcgatgttgt tcaagccttg
     8581 agcggttact gaaaaaacac cgcgatcttg ttaaacatcg tcggatcgga ctgattacgc
     8641 tgcactttca cgacatcttc cagcttagcg atttggctta tcatctgctc cagacgctgg
     8701 tcgtcattga ccagtagcca gatatggctt ttgtcgctgt cctgaatcgg cagacaaaga
     8761 atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt cattacgccc
     8821 ggatggttgc gaacggtgag ctccagaatt acgttgttat gagttgtgtt ttgcatggct
     8881 tattccccca ccatttcagt attcgccgca cctggcggca ccatcgggta aactttttct
     8941 tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc gattgatgat ttcctgcaat
     9001 gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa tccggcggca
     9061 atctgcataa agttgatttt gcccggatag gtggcggcaa aaacgccttg ctcgtagaac
     9121 agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcagaat gattttgaca
     9181 tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag gctgccgtcg
     9241 ccggagaaac acaacacttt gcgatccggg ttcgccagcg ccgcgccaat cgccgcaggc
     9301 aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc actggcgtgg gcgattgagc
     9361 ggataagctt gcgcggtcca catctgatgc tgacccacat cggtggtgat aatcgcattg
     9421 tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg atcgcacgct
     9481 ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta ccaactggtg ccactctgca
     9541 cgcggttgcg cttccacccg cgggatcaat tgcgccagca cgtcatcaac atccgcctga
     9601 atcgccacat gcggctgctt gattttaccc agctctgcgc gatcgatatc gacatgaata
     9661 attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc aaaacgcgca
     9721 ccgagcacaa tcaacaaatc cgcctcctgc aaaatatagt tggtactgcg cacgccgtgc
     9781 atccccagca tacccagcga caacggatgc gcttttggca gcatgcccag cgccattaaa
     9841 gtcatggtgg taggcagttg cgctttctcc gccagttcac gcacccgcgc aggcgcattg
     9901 atcacaccac cacccagata aagcaccggg cgtttggcag cgttaatcat tgtagctgcg
     9961 tcacgaatgc tttcttcgct aaaggcgggt gcagcggctt tttctgccac cgcgggctga
    10021 gcttcaatct caaaaaccgc cgtttgtaca tccttaggaa tgtctatcca caccgggcta
    10081 gggcggcctg attgcgcaat gcggaaggca tcgctcatga cctgtgggag ctcttcgata
    10141 tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt gtccacttct
    10201 tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca aatcagcggg
    10261 atggagtcca gccgcgcatc agcaatggcg gtcaccaggt tagtcgcacc cggcccgcta
    10321 caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc gataaacccc
    10381 gcgccctgtt catggcgagc cagaatatgg cggatttgcg tgctttggct taaggcgtcg
    10441 taaacaggca ggatagaacc gcccggaatg cccgtcacaa tcttaatgcc ctgctgttcc
    10501 aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcgatgt tgtgcccgaa
    10561 cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct taaacgaaaa
    10621 accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt gttgttccag tccctacggc
    10681 gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg cgcagttggt
    10741 agtagttttg cgttgaacat ggaagtagtc attggggacc ttgttggttt tgtgtttaac
    10801 aatatttata caagcacagc tttacagggg agacaatgga aaatttttca gcaagggaaa
    10861 attgaggggt tgatcacgtt ttgtactgaa ttgcagataa caaaaaaccc cgccggagcg
    10921 aggtttcgtc agccgcctgc ggctggtaac cgcaaagcac actgtattat gtcaacactg
    10981 aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac gtatttagtg
    11041 catagttgag tatcgttcac agtttgcgtt ttgtccaaat attactgttt atttatacag
    11101 taaacttcta taatatcact gtacgcaatg tgttatgcgg gggccgcatc gttacccggc
    11161 gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc gtgagcacac
    11221 tgtgttatgt caacaagcac aacgtttctc cttgagatac cgcgtgcaca acagctggca
    11281 acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag acgcgtatga
    11341 gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg caactgcttg
    11401 atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg ggagcgggta
    11461 accgctcccc ttttatattt agcgtgcggg ttggagtcgg atgcgatgct gacgcatctt
    11521 atccgcccta ccatccctcc cggcaacatt tattgccgct tttgtttaca tattctgccg
    11581 ctaaacaatt ccccattcct ggcgtatatc tggctaacat tcatcaatgt gatagattcc
    11641 tctcccgcat ttatgggaat gcgtagtgac ttattctaat tatttttata aaagcatccg
    11701 tgataatgaa aaggcaaaga aacgtcaatt tgttattgat gttggtatta ctcgtggccg
    11761 tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg cgcgatctca
    11821 acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact tacggtgtct
    11881 cacagctgtt ttatggcccg atatccgacc gtgtcggtcg ccgaccagtg atcctcgtcg
    11941 gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt ttgacggtat
    12001 tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta atggcgcgta
    12061 ctttgccgcg tgatttatat gaacggacac agttgcgcca tgctaacagc ctgttaaaca
    12121 tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg gatacgatgt
    12181 ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg accttcagta
    12241 tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc ctgcttacca
    12301 gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg ctgattggcg
    12361 gtctggccgg gattgctgcc tttgaagcct gctccggcgt gctgatgggc gcggtgttag
    12421 ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggcg gcattttttg
    12481 gggcatggtt tgccgggcgt cctaataaac gcttctcaac gttgatgtgg cagtcggtta
    12541 tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc gtgatgaatg
    12601 tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg ctgtttccgc
    12661 tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct ggtgcgctgg
    12721 tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg atgttgccgc
    12781 aaaccggtca gggcagcctg gggttgttga tgaccttaat gggattgttg atcgtgctgt
    12841 gctggctgcc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa gcgcacgtca
    12901 ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat cacgcatcac
    12961 ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa gcacgctgtc
    13021 tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag tcgcacgcct ggcagtcacg
    13081 gcgcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa aaagctcaat
    13141 cgagatacga ctgatatctg cccgcaccca acactcaaac agggcaatta aaaatttccc
    13201 ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa attcaagata
    13261 gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa ttgtgataaa aatcggcagg
    13321 ttgcagagca tccttgtact ctacaaccag cttatgcctg tcctcttcag catcaatgac
    13381 ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat atgcgttaca
    13441 gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc acggactacg
    13501 tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg ggtgtagtgc
    13561 cagaggatca gcgccacgat ggcgaggatg ccaatcgaaa tccagaataa catgcccgcc
    13621 tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag catggtacga
    13681 aaccacgcca gtgatgtgcg ctccggctgc agccctgggt cggcaatgcg tctggcttta
    13741 cggctatccg gcatacaaca ccagtcccat aacgatcacc accacaacca tcaatattaa
    13801 gctgatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca tcgctttttc
    13861 attgcgtaac cagcgcaggt agccatacat tgctaacccg ccggaaaaca gacacaacaa
    13921 caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt gatcaagccc
    13981 aacccccgcc gccagaaatc ctagtgcggt acggatccac gccagaaaag tacgctcatt
    14041 agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaattttca ttgttgctcc
    14101 tttgcaattt cactaacgct ctatctttag cggttttttt taagaatttc gtccgcccgt
    14161 ttcctgatat gcttttgcca tccgctacaa ggaggtcaga tgggtatcat agcgcaaaat
    14221 aaaatcagtt cattgggcat gctgttcggt gccatcgctt taatgatggg gattatccac
    14281 ttttcattcg gccctttttc cgcaccaccg ccaacgcttg aaagcatcgt ggcggacaaa
    14341 acggctgaaa ttaaaagagg tcttctcgcc ggaataaaag gcgaaaaaat taccactgtg
    14401 gagaaaaaag aggacatgga tatcgataaa atcctcgatc aaagcggtat cgcactggcc
    14461 attgctgcgc tactttgcgc atttattggc ggtatgcgta aggaaaatcg ctggggcatt
    14521 cgcggcgcgc tggtttttgg tatcggcatc gtgtgcagca ttttgctgat attcctgatt
    14581 ttttccttcc tgaccggcgg ttcgctggtt taagcagcct gaataatgcc cgccgggtga
    14641 tcatcagcgg gcattatcgt cagaatttct gttttttctc ctccaccttc actccttgcg
    14701 tcggtaggcc ggtgtcatag tcgcgtacca ctggcgaata gccatcttgt gggcgtgggc
    14761 gaaacgcccc catccagcgc ggtcgtgcat ctttacgcca cggacgcaga ctccattggt
    14821 aactgcggaa cggatcgcga attttgtcca tgtaatccaa taaggcgtca tgcattttgc
    14881 tgcgaacgtc tgcaaaacgg atatcatcga tcaggttatg catttcattt gggtcattac
    14941 ggcgatcgta aagttcatca ctggtgaaga ggttgagcac cagtttaaag tcatccgtca
    15001 cccagcaacg caccggaata aaaccgccaa agctgtcatg ctcaatctcg tagcggttaa
    15061 attccaccat cacgccgcgt ggctctttca cggcaaggat attttccccc ggcagaatct
    15121 ctggtttttc aatatctgcc agcgccatca ttgtcggcag taaatcaata tgactgactg
    15181 gcgtatcgac ctgtcgccgc tccccttgcg gcgaacggat gatcagcgga atacgggtga
    15241 tgtcgtcata catcgccgct cctttactga tcagcttatg tgcgcccatc atttcaccgt
    15301 gatcggaggt ataaatgacc cacgtatttt cacgttgctc tggcgttaag gcattgatga
    15361 cacgtccgat ttggtcatca acaaagtcat tgcaggcaaa atagagcgga tggtgataaa
    15421 gcccgtcatc accgactggc gatggcatcg cctgcgccca taagcggtga tgttccggtt
    15481 tgttcgccag gtcatcctgt gatttctcgc caagttcgta gtaaaaatca gcgtatttct
    15541 ctaaatactc caccggacag gtgaacgggt gatgcggctc atcatacgaa accaccatca
    15601 ggaagggttc ctcggcgcgc acgggctatt gcagaaaatc taccgctcga ttgctgatgc
    15661 ggtgcgccca ggtgaaggtt tcgtcaatat ggttcgcctg taaatcttcg acgctgttta
    15721 ggccattgcg ccacaggctg atctcttttt ccgtcagttc gctaagatag ttcgccccat
    15781 caaaccagta atcagcatcc cactccggcg gacactcgcc agtgccgaaa tagtcatgac
    15841 cgtcgagatg ccatttgccg atgtaacagg tgtgatagcc ggcatcctta aagtagcgcc
    15901 ccatagtgga gatgtttttg cctggcgcga cgttgttggt ccacgggccg gactggttag
    15961 cgtagatacc ggtgaatagt ccggcccgtg caggcgtaca aaccggtgaa caagtgtagg
    16021 cggaattaaa gcgaatacct tccgccgcca gactatcaat attttgcgta ttcagcggtt
    16081 taccgctata gcaaccgacc atattggtgg cctgggtatc ggtcaattgg ggcgtttcat
    16141 aggctatcct tcgcgtcggg agtaaaggtt acggctgggt cgtgacgatg ccaccagctg
    16201 tcaaaaatca ggtagacaat cactacggca gcgatgaaat aactgatcat cgctaaccag
    16261 cgcgtaccgt agccgccgaa ttcagccagc ccgacataga cgccaatcat cgcgaacagg
    16321 ataccaattg acgcgatctt gacgtttttc cacggtttca tgtccaccgc aaacgcatct
    16381 ttgaaggtga acggcgtggc gcgcggtttg ataaaaccga tcaccagcat cacgaccacg
    16441 ttgatgcaga acgtacaggc cagcacatag aggaaatgga agtcgaactt caccagatag
    16501 ttgatggtga tgtagctgat tatgccaatc cccatcgcca cttttgccgc cagcgccggg
    16561 atgcgcggaa agaaaaagcc cataatgatg atggtaacca gcggcacgtt gtaaatgccg
    16621 ttgagctgtt tcatccagct atacagcccc tgcggcgcgt tggcgatcca cggcgctacc
    16681 aaaaccgaaa cgatagcgat aaagaaacca aatttgcgcc cgacggtgac cagctgctgc
    16741 ggctcggcat tctggttaat gatgcgacgg taaataccca tactgaataa tgtactggcg
    16801 ctattcagaa agccgttgaa ggtactgatc accgcaccaa ataacaccgc gccgaagaaa
    16861 ccctccagtg gcactggcaa aacgttattg accagcgtcg ggtaggccat gtcggctttc
    16921 ggtagatcct gatacagatg aaacgcaatc aaccctggca gcaccagtac cagcgggtcg
    16981 agcattttca gcaccgccgt taacagcgcc cccttttgcc cttccgccag gctttttgac
    17041 gccagcgtgc gttgcacgat gccctgattt gtacaccagt aaaaggtgtt caccagaatt
    17101 aaaccggtaa atgccgcgcc aatcggcaag ggatcggtcg ggccaccgac tgagtttaat
    17161 ttctcggcgt gaacggtggt aatttgctca atgccctgca taaagctgcc cttgcctatc
    17221 gcgatcaggc caaataccgg caccatcaac ccgccgataa ccagcccaat accgttgatg
    17281 gagtccgcca ctgccattgc gcgcagtccg ccgatcaccg catacaaaat tcccgccaga
    17341 ccaagcaaaa ttaccagtag ccagatagcc gcaccgtgag aaatctgtag cgattccccg
    17401 acgtgaaaca ggctgttgag cgccaacgcg ccggagtaga gaacaatcgg cagaaagcag
    17461 acgccggtgg caatgaggaa gcagaagtcg atgataatac gcgtcgtttt atcataacgt
    17521 tcctccagaa aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg taaaaagatt
    17581 agcgcgagga agatcagcgt caccgctgaa gtcacttccc agcccatcac cgacatgccg
    17641 cttttgtagg cctgcccgga aagaccaacc agttgttccg tggaaaggtt ggttaacatt
    17701 aacgaagcgg caataaccgg cgcttttagt gaacgtccgg caagaaaata gccttgttgc
    17761 gatccggtat ctgttttgcg aaccttccac caggtgatca ccgccaccag cagcgtaaaa
    17821 ccgacaaaac tcaagatttg taacgaattc atctcatagc ccttatttat gtttatgtcc
    17881 cggacgcatg aatataggga gaggtaaaat gcgtcgggtc aggcagtcag attcttaaaa
    17941 tacaaacgtc gtatccctga acggattgtt ttctgttaag ttcaggttgt gagcataatt
    18001 cgccggaggg atattaaaat gaatggaaaa ttgcaaagtt cagatgtaaa aaacgaaact
    18061 ccgtacaata ttcccttatt gattaacgaa aatgtgatct ccagcggaat ttctctgatc
    18121 tcgctctggc atacctacgc cgacgagcat taccgggtga tctggccgcg ggacaagaag
    18181 aaaccgctta tcgccaactc atgggttgcg gtgtataccg tacaaggatg tgggaaaatt
    18241 cttttaaaga atggcgaaca aataactctg catagcaact gtattatatt tttaaagcca
    18301 atggatattc actcttatca ctgtgaaggt ttagtctggg aacagtactg gatggaattt
    18361 acccccacca gtatgatgga tattcccgtt ggtcagcaaa gcgttattta taatggcgaa
    18421 atttataatc aggaactcac cgaagttgct gagttaataa cttcaccaga agcaataaaa
    18481 aataatctgg cagtcgcttt tctgacgaaa attatttatc agtggatttg tcttatgtcc
    18541 gcagatggta aaaaagatcc acaacggcgg caaattgaaa aattaattgc cactttacat
    18601 gccagtctgc aacaacgctg gagcgtagct gatatggctg ccacgatccc ctgtagcgaa
    18661 gcctggttgc gtcgtctgtt tttacgctat accggcaaga cgccgaaaga atattacctc
    18721 gatgcgcgtc tggatctggc gctatcgcta ttaaagcaac aaggaaactc ggttggcgaa
    18781 gtcgctgata cgctcaactt cttcgactcc tttcatttca gcaaagcctt taaacataaa
    18841 tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac ggatgccagt
    18901 ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa ctcatccaga
    18961 atagctttgg cgaccgaggc gctcggtacc gttttcgaca acgtaatcgc ctgccacagg
    19021 tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc cacttgctga
    19081 ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc aggccgtagt
    19141 agcgcacgcg catctgtttg cgatcttgca gcccaacaat ttgcgccatc cggctttcaa
    19201 taccgattgg catgtcacag atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg
    19261 cttctgcgac aattgctgcc gggttggagt agttgagcat ctaggcgttt ggtgaatatt
    19321 tttccatata atccaccagt tccaggacgc cgccaatgga acgcatgccg tacgctattc
    19381 cgccgggtcc gcaagtttcc tgaccaacaa caccgtggcg cagtgggatt ttttcatcca
    19441 gttcgcgcat cgggtacttg ccgacgcgga tgtgcgccat gacaaaatca acgtcgctga
    19501 acgccacttc aggatcggtg gtgtaactaa acgcaatgtc cggcgctttt tctttaagga
    19561 tgactttaca ggcttcggca atcacttcct gccgcgcgcc atcgttatca taaaatttca
    19621 gtgcacgaag cgggaaacgg tcctgattcg ccaggagcat caacacgatg cctggcgtaa
    19681 aggtgcttcc accgcctgcg acaaccactg agaatttggt cattatactg cctccgtaat
    19741 ggcaacattt tctgctgatt gatgagaatt aattaagcta tcgagctgtt cacgcaattg
    19801 ggatacatgc agaccaatga tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc
    19861 tcccagcttt ttaaagactt cgtcatccag cgtttgtgac atgtcatgca gtgcaatacg
    19921 tagacgcgtc gcgcaattgt taatgctgga gatattgccg accccgccca gggcttgcag
    19981 gataccggca gcctgatcca gctctttttt tggctctgca gcggtggttt ggcctcgcga
    20041 ggctttgtat tcggcttttg agtagagttt cacttccgca tcttcacgtc ccggcgtgca
    20101 catgttgaac tgcagaatca gggtgcggaa aaccacgaag tacagcaggg taaagcacaa
    20161 cccaatggcg atttgggtca gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt
    20221 ttgcggtaaa acctggtcaa tcagacctcc gcccatgttg cccaccacac caaagagata
    20281 cattacggtc gacattgagg ccgccagcac ggcgtgtacc gcaaacagca acggtgaaat
    20341 gaacaggaag gtaaattcca gcggttcggt aattcccacc agcatggcgg ttaaggttgc
    20401 gggaatcagc aagcccgcca cttttacccg attttccggt gcggcagtga agtacatcgc
    20461 taaagaaatg cccacggcac caaagatttt tgagttaccg tgcagggcaa aaccgccttc
    20521 cgggaacaac gatttcagtg gctcggcgct cagactgaac tcttgcagat gctgcgccca
    20581 gtacatctga atgccgcctt caacagctgc cggaccaaag ataaacggtc cgtagatgaa
    20641 gtggtgtaaa ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc acaccccaag
    20701 cgcacccgcc gaacgcagga acgcttgcag agattcaatc cccatttgta cttttggcca
    20761 gccgagcaat gtcagccagg cacaggggat catcaccagg aaggcgataa tcaccacata
    20821 agacgtcccc tggaaaatgc cgagaaaaac cggcagtttt ttatcgaaca gacggttatg
    20881 cagcgccgtc acaatgccgg aaatgataat tgcgccgata atgctggtat cgagggtttt
    20941 aatcccggcc atcattgtca gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc
    21001 gaagtagctt ccccaggtca ttcccatcgc attgatgaaa tagttccagg tcaggaaact
    21061 caccatcacc gccagacaag cacgtccctg cgcttgctta gcaaggccaa tgggtaaacc
    21121 gacagcaaaa atcagcggca tattacggaa taccgtccaa ccgccctctt caataatgtg
    21181 tacgatttgc gcgaataaac tgttcggatc ggtcagtgat tccccgacaa aacatcgggt
    21241 tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg
    21301 tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca
    21361 attctgtaag taggaagcct gattggttat gcggcagacc tggagcgagg ataggaagat
    21421 gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg
    21481 gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat
    21541 cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt gtgggcgcat
    21601 tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca ttgcggagcg
    21661 gttaagaatt cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa
    21721 aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca ttgacctgct
    21781 ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga
    21841 tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac ggcagggaaa
    21901 aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag
    21961 ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga
    22021 agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc
    22081 gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat
    22141 tggcggtaat tatgaaagcc tgatgccggt gctcgccgat agactgctgg cgcggcaaat
    22201 gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg
    22261 ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg tggagtacca
    22321 tttacggcga ctccacccgg aaaaaagtta accgatactc cagaagagca ccgccagtaa
    22381 ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc
    22441 cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat
    22501 ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag
    22561 aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag
    22621 cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga
    22681 gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca
    22741 gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc
    22801 cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa
    22861 caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg
    22921 ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt
    22981 gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg
    23041 gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt
    23101 tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt
    23161 agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta aatccgctgg
    23221 ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg aaggtacttt
    23281 tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac
    23341 atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat
    23401 caacgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac
    23461 gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata
    23521 cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg
    23581 caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc
    23641 cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc
    23701 gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc
    23761 aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaactc
    23821 ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc
    23881 gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac
    23941 gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat
    24001 atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat
    24061 cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc
    24121 ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa
    24181 tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat
    24241 acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt
    24301 tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca ggctaaatgg
    24361 ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc
    24421 ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc
    24481 cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa
    24541 gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg
    24601 acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt
    24661 aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag
    24721 gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt ttttcgcttc
    24781 cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag ccccacgaac
    24841 atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta
    24901 cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc
    24961 ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta
    25021 atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg
    25081 gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga
    25141 taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca
    25201 caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga ctacgatgta
    25261 aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt gcagtttatg
    25321 gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg
    25381 cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag gccaacaccc
    25441 acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca tgatatgcag
    25501 cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg ggaaagaagg
    25561 aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa ccaactgggg
    25621 cactaagtcg ctggcgattc tcgatatgcc gtttaccgct gtaatggaca cgcttttact
    25681 gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc
    25741 caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata actgatttag
    25801 cgatcagttt cggtgatcca cagctggcca ccttccatcc acgccagcca ttgaccatcc
    25861 ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt taaatactca
    25921 acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa gcccaaccat
    25981 tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg ggttaacttg
    26041 cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt atcatcacgc
    26101 attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg cgggacgttg
    26161 actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac gacgccacgc
    26221 ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc cgatttccag
    26281 ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc gccctttggc
    26341 gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc ttcataagca
    26401 cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac cagcacacac
    26461 cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg gccaaacggt
    26521 gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat tacatggtcg
    26581 ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg gctgccaccg
    26641 cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt gctcaccttg
    26701 ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt ttcatcagga
    26761 ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt caccacgccg
    26821 acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg gatattcaca
    26881 cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa taccagccac
    26941 tggctgtcgg gcgtccaggt attggtattg gtattggtga gcaggtgatt acggggagca
    27001 aaggttatct gtttcatggc gcggtcctga tacaaaacgg ttcccgccat catacttcac
    27061 aaggacttca ctttcagaca atagtctttt gccatcgtga tgaatgtgag tgaaaagatg
    27121 gaacatttcg acgtggcgat tattggcctc ggcccggcag ggtcggcgtt ggcacgaaag
    27181 ttagccggca aaatgcaggt gatcgcgctg gataaaaagc accagtgcgg tactgaaggt
    27241 ttcagcaaac cttgcggcgg tctgctggca ccggacgcgc agcgatcttt tattcgcgat
    27301 ggactgacgc ttcctgtcga tgtgatcgcc aatccacaga ttttcagcgt caaaactgtc
    27361 gacgtcgccg catcgctcac gcgtaactac cagcgaagct atatcaatat taatcgccat
    27421 gctttcgact tgtggatgaa atcgctgatc cccgccagcg ttgaggttta tcacgatagc
    27481 ctgtgccgaa aaatctggcg tgaggatgat aaatggcatg tcatttttcg tgcagacggc
    27541 tgggagcaac atattaccgc ccgctatctg gtcggtgccg atggcgcaaa ctcgatggtg
    27601 cggcgacatc tctacccgga tcatcaaatc cgtaaatatg tcgctatcca gcagtggttc
    27661 gcagagaaac atccggtgcc gttctactcc tgcatctttg ataatgcgat aactgactgt
    27721 tactcatgga gtatcagcaa agatggttat tttatctttg gcggtgccta tccaatgaaa
    27781 gacggtcaga cgcgtttcac gacgctgaaa gagaaaatga gcgcctttca gttccagttt
    27841 ggcaaagcgg tgaaaagtga gaaatgcacg gtgttgtttc cctcacgctg gcaggatttt
    27901 gtctgtggta aggacaacgc ctttctgatt ggtgaagcgg cgggatttat cagcgccagc
    27961 tcgctggagg ggattagcta tgcgctggat agcgcagaga ttctgcgtgc ggtgttcctg
    28021 aaacagccgg agaagagcaa cgccgcttac tggcgcgcca cacacaaact gcgtttaaaa
    28081 ctcttcggca agatagtaaa aagccgatgc ctgaccgcac cggctttaag aaagtggatt
    28141 atgcgcagtg gtgtggcgca tattccacag ttgaaagatt atccaacgcg cttcacatca
    28201 cccaccagca ggatgtaaga gagcgcgcca atcaacgcga cggcggagat ataaaccagt
    28261 gcaggcgcga aaccgtaacc ctgcgccagg taccccacca ccagcggaac ggtgatgccg
    28321 cccagaccac cggcgaagtt aaacacgccg ccggttaaac caatcaggcg catcggtgcc
    28381 agagaagaga ccagcgacca ggtaatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc
    28441 atcaggcaca taatcatcat cggatcgttg gtgtagttag cgcccataat gcaggtggag
    28501 atcagcaagc cgcagataat cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt
    28561 accagcagat ccgcgaccca gccagagagc aggacgccga caaacgccgc gaggaatggc
    28621 accgtggtca taaagcctgc tttcagcgcc gtgattcctt tttcctgggt taaatagttc
    28681 gggaaccagg ttaagaaaaa ccacagtgca gaagccaccg caaattgccc aagataaacg
    28741 ccgatcagtt tacgatggaa caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc
    28801 tctttcttca ccggcgcatc accatccacc agaccgccgc catcacgaat gtaatccagt
    28861 tcagctttgc tgataccttt ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc
    28921 gaccagataa tgccgatacc accagtgaca atgaacaccc agtgccagct caacatctcc
    28981 tgaatccaga tcagcagcgg agtcaaaaac gccagaccga caaactgacc agacgtataa
    29041 aaaccaacgg cggaagcgcg ttcatgttcc gggaaccagc tggtcaccat ccggttattg
    29101 gtcgggaaag cgggcgcttc gaaaataccg gttatcgcac gcagaccaat taatgacatt
    29161 aatcccgtgg caaagccttg gaataaagtc accactgacc aaccaaatat cgcaataaaa
    29221 taagtcacgc gagaacctac gcgatctaaa aaccaaccgc cggggatctg gcatagcgta
    29281 taaagccagg cgaaggccga aaatacatag cccatttccg ctttcgtaat gccgaactct
    29341 tcctgaatat gggcggaagc cacggccaag ttagcgcggt caacataaca aatgaccacc
    29401 gtaataaaga tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg
    29461 ggaatatcca tagcgagctt tctccagatt ttgggcatag cgaagccgct caccatgccc
    29521 tgtaatttac agagggttat atttttgtat tgctgtttta gtgcccgatg aggggcttac
    29581 gtggcaggaa ttaccactct gctacgctgt tatcttcatg acgccagagc ggattacgcc
    29641 agtccggggc atttttactg aactcaataa ctttagcttc gtcgatttcc acacctaagc
    29701 ccggtttcgt taacggttta aagaagccgc caaccatgct gaagtcttct ttgtttttca
    29761 caaagtcgag taactccgcg cctttgttgt aatgaatgcc catactttgt tactgaagta
    29821 ccgcgttata ggaaacaaag tcgatatgca ggcaagccgc cagtgcaatc ggtccgaacg
    29881 gacagtgcgg cgcaagggtc acatcatagg cttctgccat tccagcaatt ttgtagcatt
    29941 cggtaatacc gcctgcatgg gagagatccg gttgcagaat cgaaataccg cctgcctcca
    30001 gcacgcgttt aaaatcgaaa cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg
    30061 tttgtgccgc caatttcggg tagtattcgg cttgttccgc cagcaccggc tcttcaataa
    30121 acagcgggcg atacggctcc agttctttaa tcagcacttt cgccattggc gcgctgacgc
    30181 gaccgtggaa atcaagacca aactcaatct gattgccaaa agcttcacga atttgtgcca
    30241 cggtgttgac tgccgcatct accgcgcggg agttatcaat tagccccagt tcttcacaac
    30301 cgttcagttt gaaggtatcg aagccgattt cgcgcagggt tttaatgccg tcgataacat
    30361 ccgccggacg atcgccgccg acccaactgt aggctttaat tttgtcgcga accaggccgc
    30421 ccatcagttg ccagactggc gcattcagca ctttgccttt gatatcccat aacgcctggt
    30481 cgatcccggc gatggcgctc atcaggattg gaccgccacg ataaaatccg gcgcgataca
    30541 tcacttgcca taagtcgttg atgcgcgaag gatcctgacc aatcaaatag tcacccagct
    30601 cgtgaactgc cgcttccacc gtacgggcgc ggccttcaat cacgggctcg ccccaaccga
    30661 ccacgccttc atcggtttca attttcagga acatccagcg gggaggtaaa cgatacgtgg
    30721 taattttggt gattttcatt gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg
    30781 ctgcgcggtg cgttctacgg actgcccggc gcgataaaga tcgctgccta agcccgcccc
    30841 aacacaacct gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa
    30901 gactgcgatg tccggtggta ataccgcttt taacgctttg atgtattgcg gaccaaaagc
    30961 cgatgacgga aatattttca gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt
    31021 cgccgtcgcg cagccggggc aaacggtcat gccgtagccc accgcgcggc ggatcacttc
    31081 actatggata ttgggcgtaa cgatgagctg acagcccatc ctggcgagcg catcgacctg
    31141 ttcaggtttc agtaccgtac ctgcgccaat caacgccttg tcgccatacg catcaacgat
    31201 ggcgggaatg ctctgctccc attgtgggga attcagcggg atttcaaccg cgtcgaaccc
    31261 ggcgtcaatc accgcgccaa catgcgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat
    31321 ggcgatcagc gggagtttag tttgccactg catgagcgat gctccttata ccagcctgaa
    31381 atgccgtgtc gcccgccacc gccgccacgt cgcaacccat cgcctgaaag gcttgctggt
    31441 agcgcgcggt cagcgatgtt ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt
    31501 cgcgcatact ggcaacctct gcgccaatca acaaaccaga gagaaattcg ctgacctgtt
    31561 cgcggggaag tgttcccagc acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg
    31621 cgggcgcatt aaggccgcgc tcaaggccag ccgcgaaggc atcggcagag ttttcctgcg
    31681 gcggcaaacc tgcgccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca
    31741 tcacggtgcg aaaatcgttg atttgctggc tatcggcctg cacccatttg caatgcgtac
    31801 cgggcataac ataaagagag gaaggagcca gagctcgcgc gccgatcaat tgtgtttctt
    31861 cgccgcgcat cacattgtgg ttatcgtcat gagagacgca taatccggga ataatccaga
    31921 tattgtcgcc aacagacgtt aattgttcgc caatagacga aaaacgggca ggaacagata
    31981 aatacggagc aactttccag ccgacattgc tgccgaccat tcctgccatc accactggcg
    32041 tattttcttc acgccagtcg gtcgtgactt ctgctaacac cacagccgga gattttccgt
    32101 tcaggcgcgt gacgcctgct tctgattgcc tgctctccag gcagtggtcg ccctgataaa
    32161 gccaggcgcg cagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat
    32221 ttcctttaac cttcgtgtcg agctggcaat catggtaagc gccgcctgct ctgccgcatc
    32281 gccgtcctga tgccgtatcg catcgaacag cgccttatgt tcctggagcg tttgcggcat
    32341 gttggcctca tcgcccatcc aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc
    32401 aatgctaagt tgctgtaaca ccgggttatg caccgactgc agcaccgcct cgtggtagcg
    32461 aatatccgct tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc
    32521 cgattcaatc tgcgccagat cgctggaggt cgcgcgctct gctgcccaac gggcaatcgc
    32581 cggttccacc agatttcgca cttcgctcat ggcactgata agccgtgggt cgtagtcgtt
    32641 ttccagcacc cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggtgccac
    32701 aaacgccccg cgataacgtt tcatttcaat cagccgcttc gccatcagcg aacggaacac
    32761 ctcacggatg atgttgcgcg aggttgcaaa ctcttcacag agctccgcct cagccggaag
    32821 tggcgagcca ggtacgtatt tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg
    32881 atcggtttta ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac
    32941 cccgatcgat gcataacgcg gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc
    33001 gcgcgcgtaa tgtagtacaa acttatattg ttatgctaca atttagatca aaaaaagaac
    33061 aatacataaa aaaatgacat gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa
    33121 caggagggaa agaagagtat gctttcaacg gcttagctac tcgtttaaag gattaatcat
    33181 gaagttgaat tttaagggat tttttaaggc tgccggttta ttcccactgg cgctgatgct
    33241 ttcaggctgt atctcgtatg ctctggtttc ccataccgca aagggtagtt caggaaagta
    33301 tcaatcgcag tcagacacca tcactgggct atcgcaggca aaagatagta atggaacaaa
    33361 aggctatgtt tttgtagggg aatcgctgga ttaccttatc actgatggtg ccgatgacat
    33421 cgttaagatg ctcaatgatc cagcacttaa ccggcacaat attcaggttg ccgatgacgc
    33481 aagatttgtt ttaaatgcgg ggaaaaagaa atttaccggc acaatatcgc tttactacta
    33541 ctggaataac gaagaagaaa aggcactggc aacgcattat ggttttgcct gtggtgttca
    33601 acactgtacc aggtcactgg aaaacctaaa aggcacaatc catgagaaaa ataaaaacat
    33661 ggattactca aaggtgatgg cgttctacca tccatttaaa gtgcgatttt atgaatacta
    33721 ttcacccaga ggcattccgg atggtgtttc cgcagcatta ctgccagtga ctgttacact
    33781 ggacatcatt actgcaccgc tgcaatttct ggttgtatat gcagtaaacc aataatcagt
    33841 aagcgggcaa acgcgtttat gctgtttgcc cgcttacaga ttaattcagc acatacttct
    33901 caatagcaaa cgccacgcca tcttcaaggt tggattttgt gacaaagttc gccacttctt
    33961 tcaccgaagg aatagcgtta tccatcgcca caccgacgcc tgcatattca atcattgcga
    34021 tgtcgttttc ctgatcgcca atcgccatga tttcttccgg tttaatacct aacacgtcgg
    34081 ccagtgattt cacacccgta cctttgttaa cgcgtttatc gaggatttcg aggaagtacg
    34141 gcgcactttt cagcacggta tatttctctt tcacttcctg cggaatacgc gcgatagcct
    34201 ggtcgaggat ggcgggttca tcaatcatca tcactttcag gaactgggta ttggggtcca
    34261 ttttctccgc ttcgcagaac accagcggaa ttgtggcaac gaaggattca tgcaccgtgt
    34321 agtagctgat atcgcggtta gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag
    34381 aaccgacttc gcgagagagt ttttccagga aacgatagtc gtcatagctg agagcggttt
    34441 gcgccacggt gctaccatca gcggccttct gtaccagcgc gccgttataa gtaatgcagt
    34501 agtcgcccgg ctgttccata tgcagctctt tcaggtagtt gtgcacacct gcatacggac
    34561 gacccgtcgt tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa
    34621 cggcgggtga aatggtgtga tcgggcagca gaagggtgcc atccatatcg atagcaatga
    34681 gtttgatagc catgagttcc caaggtagat tagttcctga cccatgctaa cgcgattccg
    34741 ctcaaaaatc agtacaacac ccgagggaaa aggggatgca acgcgggttg catgctcccc
    34801 atttgcttag cggaagagtt tccctttcag cagttccatg cctgcggaaa gcagatcgtt
    34861 attggcttgt gctgacactt caccttgcgg tgagagcgca tcaataatct tcggcaattg
    34921 ctctgccagt aaactggaag ccgtactggt atccacgcca agtttttgcc cgagatcgga
    34981 caccgcattt gtgccgagcg ccgactccag ttgctcgccg ctaactgatt gatttctctg
    35041 ttgattactc aaccaggttg agagaatggc ccctaagccg ccactttgca gtttttccag
    35101 cagcacctga atgccaccct gctcctcaac ccaacttaaa atcgcctgat atttccccgc
    35161 atcgcctttc agaaaggcac cgacaacttc atcaaaaagc cccatgataa tcacctgtaa
    35221 agcgttacgt gttgacccaa aaagtataga tctgcgggat gataattgcg gattgcagaa
    35281 ataaaaaggg cggagatgat ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg
    35341 aacgccttat ccggcctaca aaatcatgca aaatcaatat attgcaggag tctcgtagga
    35401 ctaataagcg tagcgcatca ggcacgctcg catggttaac gccattaaat atcaatattc
    35461 gccgctttca gggcgttctc ttcaataaac gcacgacgcg gttctacggc gtcgcccatc
    35521 agcgtggtga acaactggtc agcggcaatc gcatccttaa cggtaacgcg cagcatacga
    35581 cggctttccg ggtccatggt ggtttcccac agctgttccg ggttcatctc gcccagacct
    35641 ttataacgct ggatggagag gccacgacgg gactctttca ccagccagtc cagcgcctgc
    35701 tcgaagctgg ctaccggctg acgacgctcg ccacgttcga taaacgcatc ttcttccagc
    35761 aagccacgca gtttctcacc cagcgtgcag atacgacgat attcgccacc ggtgataaac
    35821 tcgtgatcca gcggatagtc agtatccaca ccgtgggtac gcacgcgaac aatcggctcg
    35881 aacaggtttt gttcggcatt ggtgtggaca tcaaacttcc actggctgcc gtgctgttct
    35941 ttgtcgttca gttcgctgac cagcgcgttc acccagcggg taacggtctg ctcatcagaa
    36001 aggtcagctt ccgtcaacgt cggctgatag ataagctctt tcagcattgc tttcggataa
    36061 cgacgctcca tgcggttgat cattttctgc gtcgcgttgt actcagatac cagtttctct
    36121 aacgcttcgc cagccaatgc cggtgcactg gcgttggtgt gcagcgttgc gccgtccagc
    36181 gcgatagaga tctggtactg atccatcgct tcgtcgtctt taatgtactg ttcctgcttg
    36241 cctttcttca ctttgtacag cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt
    36301 tccggcatct gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg
    36361 tccgcatcgg tcatgatgat gatgctgtga taacgcagtt tgtccgggtt gtactcgtca
    36421 cgaccgatac cacagccaag cgcggtgata agcgtcgcca cttcctgaga agagagcatc
    36481 ttatcgaagc gcgctttctc gacgttaagg attttaccct tcagcggcag aatcgcctgg
    36541 ttcttgcggt tacgcccctg cttcgcagag ccgcccgcgg agtccccttc caccaggtac
    36601 agttcggaaa gcgccggatc gcgttcctgg cagtctgcca gtttgcccgg caggccagct
    36661 aagtcgagcg cacctttacg gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca
    36721 cgggcagcat cgataatttt gccaaccacg attttcgcat cagttgggtt ttccagcagg
    36781 tattccgcca gcagttcgtt catctgctgt tcaaccgccg atttcacctc agaagaaacc
    36841 agtttgtctt tggtctggga ggagaatttc gggtccggca cttttacgga aacgaccgca
    36901 atcaggcctt cacgcgcatc gtcaccggtg gcgctgactt tggctttttt gctgtagcct
    36961 tctttgtcca tgtaggcatt cagggtacgg gtcatcgccg cacggaagcc tgccaggtga
    37021 gtaccgccgt cacgctgcgg aatgttgttg gtaaagcagt agatgttttc ctggaagcca
    37081 tcgttccact gcagcgccac ttcaacgcca ataccgtctt tttcggtgga gaagtagaag
    37141 atatttgggt ggatcggcgt tttgttcttg ttcagatatt caacgaacgc tttgatgccg
    37201 ccttcatagt ggaagtggtc ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg
    37261 ccggagttga ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg
    37321 gtcacattgg tgaaggtttc gaggctgggc cagaaacgca ccatggtgcc ggttttttca
    37381 gtctcgccgg taaccgccag cggagcctgc ggtacaccgt gttcgtagat ctgacggtga
    37441 attttaccct cgcgctgaat aaccagctcc agtttttgcg acagggcgtt tactaccgaa
    37501 acaccaacgc cgtgcagacc gccggacact ttataggagt tatcgtcaaa tttaccgcct
    37561 gcgtgcagaa cggtcatgat cacttccgcc gccgatacgc cctcttccgg gtgaataccg
    37621 gtcggaatgc cgcgcccgtc atcctgtaca gagacagagt tatcggcgtg aatggtgacg
    37681 ataatttctt tacagtgacc cgcgagcgct tcgtcgatag cgttatctac cacctcgaat
    37741 accatgtggt gcagaccggt gccgtcatcc gtgtcgccga tatacatacc cgggcgctta
    37801 cgcaccgcat ccagcccttt caggactttg atactggagg agtcataaga attcgacatc
    37861 aacgtttctc gctcatttat acttgggtta atctgttatt ttaccctttt ccacggtaaa
    37921 catcttcgaa ttttcgtccg acatgtctat aacgtgttca gcactgatcg cgctgacaaa
    37981 gacctgtgat tgcgtcgctt ttaagcggct ggcaagcaac ccgcgacgct catcatcaag
    38041 ctcagaggca aaatcatcta tcaggtagag acaccgccgc ccgctttcac gagtgaggaa
    38101 ctctccttgc gccagacgta aggcgcacat caacagctta agctgcccac gcgataaggt
    38161 atcttccacc ggcgcaccgt cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc
    38221 ggtgtaggtt agctggcgat cgcgttcaaa attacgttcc agcacctcag cgtactctgt
    38281 ctctttctcc cagccgcgct ggaaagagaa agtcagagaa aactcaggga gaaattgctt
    38341 acaggtatca tccatatcgg ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat
    38401 ttgctccgcc agcgggatca gttctttatc ccacgggcgt agctgttcgt aacgagtcac
    38461 ctggcgcagc gccgcattgc gctgcttgag caatcgcttg agattgctcc aggcggtgaa
    38521 aaatccaggt tcgttgtgaa agcatcccca gtcgaggaat gctcttctgt atttggggcc
    38581 gccgttgagt aaagtaaacc cttctggcgt tatcaactgc atcggcatca ggtgcgccag
    38641 ttccgcgact ttatgcccgt ccgtaccgtc aatacggact ttgctgtcgc cctgtttgtc
    38701 tttggttaag ccaatcgccg tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa
    38761 tgcctcctgc tcatggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg
    38821 gccgagcgta tagatggctt ccagcacgct ggttttgcca ctgccgttgg cacctaccag
    38881 aaagttaaag ccgggagata aagcgagatc cgcggtttca atattgcgga aatcgcggat
    38941 caacaagcgg gtgagggaca ttacagtctc attggcatga caacataggc agccgactgt
    39001 gatgcggcat cttcaatctg cacgctggaa accgaatcgg tcagcatcat gcggacgttt
    39061 tcgcatttca gcgcgttcag aacatccagc acatagctga cgttgaagcc gatttccatc
    39121 tccgcaccgc tataggtaac gtcgaggatc tcttccgctt cttcctgttc cgggttgttg
    39181 gcagtaattt tcagctggtt ttcgctgaca tacaggcgca caccgcggaa tttctcgtta
    39241 gagagaatcg ccgcgcgagc aaacgcctgc ttgagcagat cgcagcctgc ttccagatgt
    39301 ttgtccgggt tcttcggcag aacgcgacgg taatccggga agcgaccatc caccagtttc
    39361 gaggtgaaaa taaagtcgcc aacgtgggca cgaatgttat tgctgccaat ctgcacacgc
    39421 agcagattgt cgccgccgtc gagcatacgc atcagttcaa tcacgccttt acgcggtacg
    39481 atcaccgaat ggcttggcaa agattgacca attggcattg aacagaccgc cagacggtgg
    39541 ccgtcggttg ccacggtgcg cagttcttca ccttcggttt caaacagcat accgtttaag
    39601 taatagcgaa catcctgatg cgccatcgaa aactgggttg cttcaatcag acgcttcatc
    39661 gtcgcctgcg gcagggtaaa ttcgacttcg ctctgccagt catcgaggtt cgggaaatcc
    39721 gccgctggca gggtagacag cgaaaaacgg ctacgcccgg agcgcaccag catccgttca
    39781 ccttccagct gcacggcaat ttccgcgcct tcaggcagac cacggcagat atcaaagaat
    39841 ttgcgcgccg gaacggtcgt cgctcctggc tcgtgtggct gaaccagcgc aacgcgtgcc
    39901 accatttcca tctcgagatc agtaccggtc agcgacaacg taccgtcagc aacctgtaac
    39961 agcagattac cgagaatcgg tagcgtagga cgaccaccta acggaccgct cacctgttgt
    40021 agcggtttta ataaatgctc acgttctacg gtaaatttca taggtttacg atgacaatgt
    40081 tctgattaaa tttgaaaaat cttctttgat atcgtggctc tcttcacgca actgctcgat
    40141 cttacggcag gcatgaagca ccgtcgtgtg gtcacgccca ccaaacgcat cgccaatctc
    40201 tggcagactg tggttagtca gctctttcgc tagcgccatc gccatctggc gcggacgcgc
    40261 caccgagcgg gatcgacgct tggaaaggag atccgcgact ttgatcttgt agtactccgc
    40321 caccgtcttc tgaatattgt cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg
    40381 cagcgcctca cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg cgttagcaat
    40441 aacgcggttc agcgcccctt ccagctcacg tacgttagat cgtagacgct tggcgataaa
    40501 gaacgccact tcacccggca aacgaatgtc attttcgtcg gcctttttca tcaggatcgc
    40561 cacacgggtt tccagctctg gcggttcgat cgccacagtc agtccccaac cgaagcggga
    40621 tttcaaacga tcctcaacgc cgttgatctc tttcggatag cgatccgagg tgagaatgat
    40681 ctgttgatta ccttccagca gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc
    40741 tttattagca aaaaactgaa tatcgtcgat cagcagtgca tctacggaac ggtagtagcg
    40801 tttaaactct tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa caaagcgctc
    40861 ggagtgcata taaaccactt tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc
    40921 atgcagcagg tgagttttac ccagacccgt gccgccataa aggaacaacg ggttataggc
    40981 accgccaggg ttatccgcca cctggcaagc cgccgcgcgc gccagttggt tagatttacc
    41041 ttcaacgaag ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg tcggttctgc
    41101 cggggccggg acgttatccc aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt
    41161 ttgcgccacc tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt
    41221 caccggtttg gtgccgactt caaaacgcag ctgtggggca tccgctccgc agaaactggt
    41281 tagcagtcca ttgatattat taaggtactt gtcccgtacc caatcgagga caaaacggtt
    41341 tggcgcgtac agggccagcg tgttatcgct cagttccgcc tgcaatgggc gtatccacat
    41401 actgaattct gtggctggta actcatcctg caatcgggca agacactgct gccaaagcga
    41461 aagtgacacg gcggactcca ctcgaacaaa agtcgataat gactaaggct gaaacattca
    41521 tgattgttga cgtacgtcga aaagaccctg cttgagggtg acgcacgaac cgctgtctgc
    41581 ggttatatgc ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat agcctaaact
    41641 gcgcaagaga tcttctgttt ctcacagatt ttccctattt atccacagga ctttccagaa
    41701 ctcgctaagt gtaaacgatc ctgccgcgag gcgggcacga tttacgccgc atattggaaa
    41761 atttaatgac catagacaaa aattggctta atcgatctaa taaagatcca ggacgatcct
    41821 tgcgctttac ccatcagccc gtataatcct ccacccggcg cgccatgctg gtttccactg
    41881 gtgtgaggtc gtacattttc cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag
    41941 agaattgact ccggtgtgta caattattac aatccggcct ctttaatcac ccatggcttc
    42001 ggtgtccatc gtttcatttt ttcggcggat atccaataaa gccattgaat ttattcaagt
    42061 ttaggtagaa atcgccatga aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc
    42121 tcacggcttc cgtgctcgta tggctactaa aaatggtcgt caggttctgg cacgtcgtcg
    42181 tgctaaaggc cgcgctcgtc tgaccgtttc taagtaataa agctaacccc tgagtggtta
    42241 agctcgcatt tcccagggag ttacgcttgt taactcccag tcaattcaca ttcgtcttcc
    42301 agcagccaca acgggctggc acgccgcaaa ttaccattct cggccgcctg aattcgctgg
    42361 ggcatccccg tatcggtctt acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca
    42421 atcggattaa acgcctgacg cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta
    42481 tggatttcgt ggtggtggcg aaaaaagggg ttgccgacct cgataaccgt gctctctcgg
    42541 aagcgttgga aaaattatgg cgccgccact gtcgcctggc tcgcgggtcc tgatagccct
    42601 cattcgggtc tatcaacgcc tgattagtcc gctactcggg ccgcattgtc gtttcactcc
    42661 aacctgttca agctacggaa ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg
    42721 gttgacggtg aaacgcgtat taaaatgcca ccctttacac cctggtggtg acgatcccgt
    42781 cccgcccgga ccatttgata ccagagaaca ctaacgatgg attcgcaacg caatctttta
    42841 gtcatcgctt tgctgttcgt gtctttcatg atctggcaag cctgggagca ggataaaaac
    42901 ccgcaacctc aggcccaaca gaccacgcag acaacgacca ccgcagcggg tagcgccgcc
    42961 gaccagggcg taccggccag tggccagggg aaactgatct cggttaagac cgacgtgctt
    43021 gatctgacca tcaacacccg tggtggtgat gttgagcaag ctctgctgcc tgcttacccg
    43081 aaagagctga actctaccca gccgttccag ctgttggaaa cttcaccgca gtttatttat
    43141 caggcacaga gcggtctgac cggtcgtgat ggcccggata acccggctaa cggcccgcgt
    43201 ccgctgtata acgttgaaaa agacgcttat gtgctggctg aaggtcaaaa cgaactgcag
    43261 gtgccgatga cgtataccga cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa
    43321 cgtggtgatt acgctgtcaa cgtcaactac aacgtgcaga acgctggcga gaaaccgctg
    43381 gaaatctcct cgtttggtca gttgaagcaa tccatcactc tgccaccgca tctcgatacc
    43441 ggaagcagca acttcgcact gcacaccttc cgtggcgcgg cgtactccac gcctgacgag
    43501 aagtatgaga aatacaagtt cgataccatt gccgataacg aaaacctgaa catctcttcg
    43561 aaaggtggtt gggtggcgat gctgcaacag tatttcgcga cggcgtggat cccgcataac
    43621 gacggtacca acaacttcta taccgctaat ctgggtaacg gcatcgtcgc tatcggctat
    43681 aaatctcagc cggtactggt tcagcctggt cagactggcg cgatgaacag caccctgtgg
    43741 gttggcccgg aaatccagga caaaatggca gctgttgctc cgcacctgga tctgaccgtt
    43801 gattacggtt ggttgtggtt catctctcag ccgctgttca aactgctgaa atggatccat
    43861 agctttgtgg gtaactgggg cttctccatt atcatcatca cctttatcgt tcgtggcatc
    43921 atgtacccgc tgaccaaagc gcagtacacc tccatggcga agatgcgtat gctgcagccg
    43981 aagattcagg caatgcgtga gcgtctgggc gatgacaaac agcgtatcag tcaggaaatg
    44041 atggcgctgt acaaagctga gaaggttaac ccgctgggcg gctgcttccc gctgctgatc
    44101 cagatgccaa tcttcctggc gttgtactac atgctgatgg gttccgttga actgcgtcag
    44161 gcaccgtttg cactgtggat ccacgacctg tcggcacagg acccgtacta catcctgccg
    44221 atcctgatgg gcgtaacgat gttcttcatt cagaagatgt cgccgaccac tgtgaccgac
    44281 ccgatgcagc agaagatcat gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg
    44341 ttcccgtcag gtctggtact gtactatatc gtcagcaacc tggtaaccat tattcagcag
    44401 cagctgattt accgtggtct ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc
    44461 tgattcggtg agttttcgct aaaataaggg cggtcagttg accgcctttt ttcttttcgt
    44521 agggcggata agcaccgcgc atccgccaca caaagcaaca ggaacatcat gagcgataat
    44581 gacactatcg tagcccaggc cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc
    44641 tccggcttca aagcccgtga agttgccgaa accgtgctgg gtaaactgcc taagccgcgc
    44701 tacgccgatt atcttccgtt taaagacgcc gacggcagcg tgctcgatca ggggattgcg
    44761 ctatggttcc ctggcccgaa ctcgttcacc ggcgaagatg tgctggaact gcaaggtcat
    44821 ggcggtccgg tgatcctcga cctgctgtta aaacgcattc tgaccattcc cggcctgcgg
    44881 attgctcgcc ctggtgagtt ttccgaacgc gcgtttctta acgataaact tgacttagcc
    44941 caggccgagg cgattgccga tcttatcgac gccagttcgg aacaggcggc ccgttcggca
    45001 cttaactcgc tgcaaggcgc attctccgca cgggttaatc atctggtaga agccctcacc
    45061 cacttgcgca tttacgtcga agcggcaatt gatttccctg atgaagagat cgatttcctc
    45121 tccgacggca aaattgaagc ccagcttaat gacgtgatgg ccgatctcga tgcagtgcgt
    45181 gctgaagcac gtcagggtag tttgttgcgc gaagggatga aagtagtgat tgccggacgt
    45241 cctaatgccg gtaaatcgag cctgttaaac gcgctggcgg gccgtgaagc ggcaatcgta
    45301 accgatatcg ccggaaccac gcgtgacgtg ctgcgtgagc atatccacat tgacggaatg
    45361 ccgctgcata tcatcgacac tgccgggcta cgtgaggcca gtgacgaagt ggaacgtatt
    45421 ggtatcgagc gcgcgtggca ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat
    45481 ggcaccacga cagacgccgt tgatccggca gagatctggc cggaatttat cgcccgtctg
    45541 cccgcgaaac tgccgatcac cgtggtacgc aataaagccg atatcaccgg cgaaacgctg
    45601 ggaatgagtg aagtgaacgg tcacgcgtta attcgtctct cggcaaggac tggtgaaggc
    45661 gtggacgtgc tgcgtaacca tctcaaacag agcatgggct ttgacaccaa tatggaaggc
    45721 ggcttcctgg cgcgccgtcg ccacttacag gcgctggaac aggcagcgga gcatttgcag
    45781 caaggtaaag cgcaactgtt gggagcctgg gcaggtgaac tgctggcgga agagttgcgc
    45841 ctggcacagc agaacttaag cgaaatcacc ggggaattta cttcagacga cctgctgggg
    45901 cggattttct ccagcttctg tattggtaag taaccgcgct tacgaagccg tattctgact
    45961 gtcagatgcg gcttcgcttc attgttagca ctcctgttat gcctcaaccc ttttttaaac
    46021 attaaaattc ttacgtaatt tataatcttt aaaaaaagca tttaatactg ctccccgaac
    46081 gattgtgatt cgattcacat ttaaacaatt tcagaataga caaaa
//
LOCUS       DAJVRL010000023        45708 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVRL010000023 DAJVRL010000000
VERSION     DAJVRL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.023"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            114..2267
                     /locus_tag="OQN02_001245"
     CDS             114..2267
                     /locus_tag="OQN02_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1642111.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OQN02_001246"
     CDS             2320..4050
                     /locus_tag="OQN02_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642112.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OQN02_001247"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OQN02_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1642113.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OQN02_001248"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OQN02_001248"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1642114.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OQN02_001249"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OQN02_001249"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1642115.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OQN02_001250"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OQN02_001250"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1642116.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OQN02_001251"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OQN02_001251"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1642117.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OQN02_001252"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OQN02_001252"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1642118.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OQN02_001253"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OQN02_001253"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1642119.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OQN02_001254"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OQN02_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1642120.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OQN02_001255"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OQN02_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1642121.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OQN02_001256"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OQN02_001256"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1642122.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OQN02_001257"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OQN02_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1642123.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OQN02_001258"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OQN02_001258"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1642124.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OQN02_001259"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OQN02_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1642125.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OQN02_001260"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OQN02_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1642126.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OQN02_001261"
     CDS             16308..16847
                     /locus_tag="OQN02_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1642127.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OQN02_001262"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OQN02_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1642128.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OQN02_001263"
     CDS             complement(18206..18553)
                     /locus_tag="OQN02_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1642129.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OQN02_001264"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OQN02_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1642130.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OQN02_001265"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OQN02_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1642131.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OQN02_001266"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OQN02_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1642132.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OQN02_001267"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OQN02_001267"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1642133.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OQN02_001268"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OQN02_001268"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1642134.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OQN02_001269"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OQN02_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OQN02_001270"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OQN02_001270"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS1642135.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OQN02_001271"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OQN02_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1642136.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OQN02_001272"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OQN02_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1642137.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OQN02_001273"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OQN02_001273"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1642138.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OQN02_001274"
                     /pseudo
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OQN02_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OQN02_001275"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OQN02_001275"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1642139.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OQN02_001276"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OQN02_001276"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1642140.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OQN02_001277"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OQN02_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1642141.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OQN02_001278"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OQN02_001278"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1642142.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OQN02_001279"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OQN02_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1642143.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OQN02_001280"
     CDS             complement(40011..40466)
                     /locus_tag="OQN02_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642144.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OQN02_001281"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OQN02_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OQN02_001282"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OQN02_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1642145.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OQN02_001283"
     CDS             complement(41309..41986)
                     /locus_tag="OQN02_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1642146.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OQN02_001284"
     CDS             complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OQN02_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1642147.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OQN02_001285"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OQN02_001285"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1642148.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OQN02_001286"
     CDS             complement(43192..43650)
                     /locus_tag="OQN02_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1642149.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OQN02_001287"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OQN02_001287"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1642150.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..45696)
                     /gene="adrA"
                     /locus_tag="OQN02_001288"
                     /gene_synonym="dgcC"
     CDS             complement(44596..45696)
                     /gene="adrA"
                     /locus_tag="OQN02_001288"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1642151.1"
                     /translation="MNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLPRAVGL
                     AGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNLKTDAV
                     LAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITV
                     SFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRH
                     WETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRG
                     SDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLN
                     PQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11879 a        11921 c        11183 g        10725 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgct
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaattgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgcc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttt cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccaag gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacac cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc tagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat cagatggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atcgtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtggcg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat agtcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aagcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttatt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactat ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gagatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttgaaaa agttttcatc attcattatt tttgggaa
//
LOCUS       DAJVRL010000024        44028 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVRL010000024 DAJVRL010000000
VERSION     DAJVRL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.024"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            48..122
                     /locus_tag="OQN02_001289"
     tRNA            48..122
                     /locus_tag="OQN02_001289"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80..82,aa:Gln,seq:ctg)
     gene            complement(276..1451)
                     /gene="ubiF"
                     /locus_tag="OQN02_001290"
     CDS             complement(276..1451)
                     /gene="ubiF"
                     /locus_tag="OQN02_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1642152.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1597..3021
                     /gene="miaB"
                     /locus_tag="OQN02_001291"
     CDS             1597..3021
                     /gene="miaB"
                     /locus_tag="OQN02_001291"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1642153.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3174..4214
                     /locus_tag="OQN02_001292"
     CDS             3174..4214
                     /locus_tag="OQN02_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1642154.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4211..4678
                     /gene="ybeY"
                     /locus_tag="OQN02_001293"
     CDS             4211..4678
                     /gene="ybeY"
                     /locus_tag="OQN02_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1642155.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4768..5646
                     /gene="corC"
                     /locus_tag="OQN02_001294"
     CDS             4768..5646
                     /gene="corC"
                     /locus_tag="OQN02_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1642156.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5671..7209
                     /gene="lnt"
                     /locus_tag="OQN02_001295"
     CDS             5671..7209
                     /gene="lnt"
                     /locus_tag="OQN02_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1642157.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7606..8514
                     /gene="gltI"
                     /locus_tag="OQN02_001296"
     CDS             7606..8514
                     /gene="gltI"
                     /locus_tag="OQN02_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1642158.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8684..9424
                     /gene="gltJ"
                     /locus_tag="OQN02_001297"
     CDS             8684..9424
                     /gene="gltJ"
                     /locus_tag="OQN02_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1642159.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9424..10098
                     /gene="gltK"
                     /locus_tag="OQN02_001298"
     CDS             9424..10098
                     /gene="gltK"
                     /locus_tag="OQN02_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1642160.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10098..10823
                     /gene="gltL"
                     /locus_tag="OQN02_001299"
     CDS             10098..10823
                     /gene="gltL"
                     /locus_tag="OQN02_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1642161.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10941..11876
                     /gene="rihA"
                     /locus_tag="OQN02_001300"
     CDS             10941..11876
                     /gene="rihA"
                     /locus_tag="OQN02_001300"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1642162.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11960..13621
                     /gene="hscC"
                     /locus_tag="OQN02_001301"
                     /pseudo
     CDS             11960..13621
                     /gene="hscC"
                     /locus_tag="OQN02_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13681..15132)
                     /gene="djlC"
                     /locus_tag="OQN02_001302"
     CDS             complement(13681..15132)
                     /gene="djlC"
                     /locus_tag="OQN02_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS1642163.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15129..15836)
                     /locus_tag="OQN02_001303"
     CDS             complement(15129..15836)
                     /locus_tag="OQN02_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1642164.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15938..16492
                     /locus_tag="OQN02_001304"
     CDS             15938..16492
                     /locus_tag="OQN02_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1642165.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16502..17929)
                     /locus_tag="OQN02_001305"
     CDS             complement(16502..17929)
                     /locus_tag="OQN02_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1642166.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17926..18633)
                     /locus_tag="OQN02_001306"
     CDS             complement(17926..18633)
                     /locus_tag="OQN02_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1642167.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18797..19774
                     /locus_tag="OQN02_001307"
     CDS             18797..19774
                     /locus_tag="OQN02_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1642168.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19844..20326)
                     /locus_tag="OQN02_001308"
     CDS             complement(19844..20326)
                     /locus_tag="OQN02_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1642169.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20561..23143
                     /gene="leuS"
                     /locus_tag="OQN02_001309"
     CDS             20561..23143
                     /gene="leuS"
                     /locus_tag="OQN02_001309"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1642170.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23158..23739
                     /gene="lptE"
                     /locus_tag="OQN02_001310"
     CDS             23158..23739
                     /gene="lptE"
                     /locus_tag="OQN02_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1642171.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23739..24770
                     /gene="holA"
                     /locus_tag="OQN02_001311"
     CDS             23739..24770
                     /gene="holA"
                     /locus_tag="OQN02_001311"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1642172.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24772..25413
                     /gene="nadD"
                     /locus_tag="OQN02_001312"
     CDS             24772..25413
                     /gene="nadD"
                     /locus_tag="OQN02_001312"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1642173.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25437..26048
                     /locus_tag="OQN02_001313"
     CDS             25437..26048
                     /locus_tag="OQN02_001313"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCS1642174.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26308..26625
                     /gene="rsfS"
                     /locus_tag="OQN02_001314"
                     /gene_synonym="rsfA"
     CDS             26308..26625
                     /gene="rsfS"
                     /locus_tag="OQN02_001314"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1642175.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26629..27096
                     /gene="rlmH"
                     /locus_tag="OQN02_001315"
     CDS             26629..27096
                     /gene="rlmH"
                     /locus_tag="OQN02_001315"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1642176.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27127..29028
                     /gene="mrdA"
                     /locus_tag="OQN02_001316"
     CDS             27127..29028
                     /gene="mrdA"
                     /locus_tag="OQN02_001316"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1642177.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29031..30143
                     /gene="mrdB"
                     /locus_tag="OQN02_001317"
                     /gene_synonym="rodA"
     CDS             29031..30143
                     /gene="mrdB"
                     /locus_tag="OQN02_001317"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1642178.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30154..31242
                     /gene="rlpA"
                     /locus_tag="OQN02_001318"
     CDS             30154..31242
                     /gene="rlpA"
                     /locus_tag="OQN02_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1642179.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31381..32592
                     /gene="dacA"
                     /locus_tag="OQN02_001319"
     CDS             31381..32592
                     /gene="dacA"
                     /locus_tag="OQN02_001319"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1642180.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32703..32966
                     /locus_tag="OQN02_001320"
     CDS             32703..32966
                     /locus_tag="OQN02_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1642181.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33067..33708
                     /gene="lipB"
                     /locus_tag="OQN02_001321"
     CDS             33067..33708
                     /gene="lipB"
                     /locus_tag="OQN02_001321"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1642182.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33967..34920
                     /locus_tag="OQN02_001322"
     CDS             33967..34920
                     /locus_tag="OQN02_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1642183.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35129..36094
                     /gene="lipA"
                     /locus_tag="OQN02_001323"
     CDS             35129..36094
                     /gene="lipA"
                     /locus_tag="OQN02_001323"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1642184.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36195..36398)
                     /gene="tatE"
                     /locus_tag="OQN02_001324"
     CDS             complement(36195..36398)
                     /gene="tatE"
                     /locus_tag="OQN02_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1642185.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36527..37315)
                     /locus_tag="OQN02_001325"
     CDS             complement(36527..37315)
                     /locus_tag="OQN02_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS1642186.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37408..37791
                     /gene="crcB"
                     /locus_tag="OQN02_001326"
     CDS             37408..37791
                     /gene="crcB"
                     /locus_tag="OQN02_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1642187.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37845..38054)
                     /gene="cspE"
                     /locus_tag="OQN02_001327"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37845..38054)
                     /gene="cspE"
                     /locus_tag="OQN02_001327"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1642188.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38229..38789)
                     /gene="pagP"
                     /locus_tag="OQN02_001328"
     CDS             complement(38229..38789)
                     /gene="pagP"
                     /locus_tag="OQN02_001328"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1642189.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39377..40762
                     /gene="dcuC"
                     /locus_tag="OQN02_001329"
     CDS             39377..40762
                     /gene="dcuC"
                     /locus_tag="OQN02_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1642190.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLDRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(40803..41144)
                     /locus_tag="OQN02_001330"
                     /pseudo
     CDS             complement(40803..41144)
                     /locus_tag="OQN02_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41482..41991)
                     /locus_tag="OQN02_001331"
     CDS             complement(41482..41991)
                     /locus_tag="OQN02_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1642191.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42154..42399
                     /locus_tag="OQN02_001332"
     CDS             42154..42399
                     /locus_tag="OQN02_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1642192.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42374..44005
                     /locus_tag="OQN02_001333"
                     /pseudo
     CDS             42374..44005
                     /locus_tag="OQN02_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        11087 a        11006 c        10981 g        10954 t
ORIGIN      
        1 attctgaatg tgtcgaatac gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca
       61 agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc
      121 cacattaaaa aagctcgctt cggcgagctt tttgcttttc tgcgttcatt caatgtcgaa
      181 tgcgatgttg acacgtctta tccttcaatg tcggatgcga cgctgccgcg tcttatccga
      241 cctacggctg gcacgcatcc ggcaatgttg taaggctaca accctaacgc atatttcagc
      301 gcctgacgtt tcaacacgcc agcacgctct gccgccatta acccgagatt acgcacaaaa
      361 cgcagcggcg gcagattatt actgaatccg gcataaaaca gatccatacc actttgcata
      421 atgaagttat ccgccatgcg ccgcatctgg taacgcttga ggacaggata actggcccac
      481 gcttcaccgt agctgcgggc attcaccaga acatcaatca gggcatcgac atcacgataa
      541 ccaagattca ccccctgccc cgccagcgga tggatggtat gcgcggcatc gcccaccagc
      601 gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg tcagcggaaa cgcaccagcg
      661 gcaagcggtg taacgtaacc cagacgcgac gggaaatgct tcgcgatttc cgcctggagt
      721 tgcgccatat tcatattctg caactggcga atacgcgccg gagagtcata ccacaccagc
      781 gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc cgtccggggt aaattgctgc
      841 caggtgctgt cgccgggatc gttctcacac tgtacgctaa tcaacatgca cgactgcgca
      901 tactgccagg catgaacgcc aattcccgcc atctgccgca cctgagaatt tgcgccgtcg
      961 gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct tcagttccag ctcctgaaga
     1021 tcattatctc gatgtaatgc aatcagcgag gttggcacac gtaacgttac tttcggatgc
     1081 gcttccagcg cctgccacaa cgcctgttgc aggactgtgt tttccaccat gtagccaagc
     1141 aacggcaact ttaattcggc tgcgtcaaat accacatgtg ccgtttccca ctcccaggtt
     1201 tccagtctgc ggtaaggatg gcaacgcata gcctgtactg catcccagac ccctaaccct
     1261 ttaagcaatg ataccgaagc cgcgctgatc gccgagatcc gcacgtccgg ttggctgtca
     1321 gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta ccgaaaatcc gtgctgtgcc
     1381 agccccagcg ccagtgcgcc gccgaccatt cctccgccga caatggcaat ttccgttggt
     1441 tgatttgtca tggtcaatca tcctgttagc aatatgcttt aagtttacag gatttttacc
     1501 cccaagggct gataacgctc atactggtca caacggcagc aaagcattac actatgcgcc
     1561 ctgcattcct ggctactatt tcgcaagagc aagtcgatga ccaaaaaact ccatattaaa
     1621 acctggggct gtcagatgaa cgagtacgat tcatcgaaga tggccgatct gctggatgcc
     1681 acccacggct atcaactgac cgacgtggcg gaagaagcgg atgtgctact gctgaacacc
     1741 tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc agttgggtcg ctggaaactg
     1801 ttaaaagaga agaatccaga cctgattatc ggcgtcggtg gctgcgtggc atcgcaagaa
     1861 ggcgagcaca ttcgccagcg cgcccactat gtcgatatta tttttgggcc gcaaacgctg
     1921 caccgtctgc cggagatgat caactccgtg cgcggcgacc gcagcccggt tgtagatatc
     1981 agcttcccgg aaatcgagaa gtttgaccgt ctgccggaac cgcgcgccga agggccgacc
     2041 gcgtttgtct ccatcatgga aggctgcaat aaatattgca cctactgcgt ggtgccttac
     2101 acccgtggtg aagaggtaag ccgtccgtct gacgatattc tgtttgagat tgcccagctt
     2161 gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga atgtgaatgc ctggcgtggt
     2221 gagaactacg acggcaccac cggatcgttt gccgatctgt tgcgtctggt tgctgcgatc
     2281 gacgggatcg atcgtattcg ctttaccacc agccatccga tcgaattcac cgacgatatc
     2341 atcgaagtgt accgcgacac gcctgagctg gtgagcttcc tgcatctgcc ggtacagagc
     2401 ggttccgatc gcattctgaa cctgatggga cgtacccata cggcgctgga gtacaaagcg
     2461 atcatccgta aactgcgtgc ggcgcgtccg gatattcaga tcagttctga cttcattgtt
     2521 ggcttccctg gcgaaaccac cgaagacttc gagaaaacga tgaagctgat tgccgacgtc
     2581 aatttcgaca tgagctacag ctttatcttc tctgcacgtc caggaacgcc tgccgccgat
     2641 atggttgatg atgttccgga agaagagaag aagcagcgtc tgtatattct gcaagaacgt
     2701 atcaatcagc aagcgatggc atggagccgt cggatgctcg gcaccaccca gcgtattctg
     2761 gtagaaggga cttcgcgtaa gagcatcatg gagctttccg ggcgtaccga aaataaccgc
     2821 gtggtcaact tcgagggcac accggatatg atcggtaaat tcgtcgatgt agaaattacc
     2881 gacgtctacc cgaactctct gcgcggtaaa gtggtgcgaa ctgaagatga aatgggtctg
     2941 cgcatggcag aaacaccgga gtcggtgatt gcccgcaccc gcaaagaaaa cgaccttggc
     3001 gtgggttatt atcagccgta attctcaggc ctgccgttcc ggtgggcctt gttttttctc
     3061 tctttatccc catctttatc gcaatgcttg cgcctttttc ctgtggcgtg aataatttcc
     3121 ttataggccg gtgaatgttc agcttccggc ggcatacaga taagaggaac ggtttgaaca
     3181 tagacactcg cgaaatcacc ctggagccag cagacaatgc gcgtctgttg agcctgtgcg
     3241 gcccgtttga tgacaacatc aagcagctcg aacgccgtct cggcatcgag atcaatcgcc
     3301 gcgataacca ctttaaactg accggccgtc cgatttgcgt caccgctgcg gcagacattc
     3361 tgcgtagcct gtatgtcgat actgccccga tgcgcggtca gattcaggat atcgaaccgg
     3421 aacagatcca ccttgcgatt aaagaagcgc gggtactgga gcaaagcgcg gagagcgtgc
     3481 cggagtacgg caaagcggtc aatatcaaaa ctaaacgcgg cgtaattaag ccgcgcacgc
     3541 caaaccaggc gcagtacatc gccaatattc tcgaccatga cattaccttc ggcgttggcc
     3601 cggcgggtac gggtaaaacc tacctggcag tggctgcggc agttgatgcc ctggagcgtc
     3661 aggaaattcg ccgtattctg ctgactcgtc cggcggtcga agccggtgag aaactgggct
     3721 tcctgcctgg cgatttaagc cagaaagtag acccgtatct gcgcccactg tacgacgcgc
     3781 tgtttgaaat gctgggcttt gagaaagtcg agaaactgat tgagcgcaac gttattgaag
     3841 tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc attctcgatg
     3901 agagccagaa cactaccatc gaacagatga agatgttcct gacccgtatc ggttttaact
     3961 caaaagcggt tatcaccggc gacgtcacac agatcgacct gccgcgtaat actaaatcag
     4021 gcttacgtca tgccattgaa gtgctggccg atgtcgaaga gatcagcttt aacttcttcc
     4081 acagcgaaga cgtggttcgt cacccggtgg tggcgcgtat cgttaacgct tatgaagcct
     4141 gggaagaagc agaacaaaaa cgtaaagcgg cgctggcagc agaacgcaag cgcgaagaac
     4201 aggaacaaaa atgagtcagg tgatcctcga tttacaactg gcatgtgaag ataattccgg
     4261 gttaccggaa gagagccagt ttcaaacatg gctgaatgcg gtgatcccgc agtttcagga
     4321 agagtcggaa gtgactattc gcgtggtcga taccgccgaa agccacagtc tgaatctgac
     4381 ctatcgcggt aaggataagc cgaccaacgt gctctccttc ccgtttgaag tgccgcctgg
     4441 catggagatg tcgctactgg gcgatctggt tatctgccgt caggtggttg agaaggaagc
     4501 tcaggagcaa ggcaaaccac tggaggcgca ttgggcgcat atggtggtgc acggcagtct
     4561 gcatttgtta ggttacgatc acattgaaga tgacgaagca gaagaaatgg aagccctcga
     4621 aacagagatt atgcttgctc tgggctatga ggatccgtac attgccgaga aagaataatt
     4681 tgccaggcat ttgactggca gacgtccccg ccgttggtga ttaacgacgg cgttttgatt
     4741 aactaacatg agaacccata cgacgccatg agcgacgaca attcacacag tagtgacacg
     4801 ataagcaaca agaagggatt tttctccctg ttactcagcc aacttttcca cggtgaaccg
     4861 aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg ggcagaacga ccttatcgac
     4921 gaagatacgc gcgatatgct cgaaggggtg atggacatcg cagaccaacg cgttcgcgac
     4981 atcatgatcc cccgctccca gatgattacc ctgaaacgca accagacgct ggacgaatgc
     5041 cttgatgtca tcatcgagtc cgcccactca cgtttcccgg tgattagcga agacaaagat
     5101 cacattgaag ggattctgat ggcgaaagac ttgctgccgt ttatgcgcag cgatgctgaa
     5161 gccttcagca tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag taagcgcgta
     5221 gaccggatgc tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt tattgacgaa
     5281 ttcggtgggg tttccggtct ggtgaccatt gaagacatcc tggaactgat tgttggtgag
     5341 attgaagacg agtatgacga agaagatgat atcgacttcc gtcagctgag tcgtcatacc
     5401 tggaccgtgc gcgcactggc ttccattgaa gacttcaacg aagcgttcgg cacccacttt
     5461 agcgatgaag aagtcgacac tatcggtggt ctggtgatgc aggcgtttgg gcatcttccg
     5521 gcgcgtggcg aaactatcga catcgacggt taccagttca aagtggcgat ggccgatagt
     5581 cggcgtatta ttcaggttca tgtcaaaatc ccggatgact caccccagcc aaagctggat
     5641 gaataaaacc gaaactggat agataactac atggcttttg cctcattaat tgaacgccag
     5701 cgtattcgcc tgctgctggc gttattattc ggtgcctgcg gaacgctggc cttctctcct
     5761 tacgacgtct ggcctgcggc gattatttcg ctgatggggc ttcaggcgtt gacctttaac
     5821 cgtcgcccac tccagtctgc cgctattggc ttttgctggg gatttggcct ctttggcagc
     5881 ggtattaact gggtctatgt cagcatcgcg acctttggcg ggatgcctgg cccggttaac
     5941 atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt ataccggact gtttgctggc
     6001 gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg tggcgattgc cacccctgct
     6061 ctctggcaag tgaccgagtt tctgcgcggt tgggtgctga ctggcttccc gtggttacag
     6121 ttcggctata gccagattga tggcccgtta aaagggctgg caccgctaat gggcgtggaa
     6181 gccattaact tcctgctgat gatggttagt ggcctgctgg cactggcgtt ggtcaaacgc
     6241 aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc tacccttccc gctgcgttac
     6301 atccaatggt ttaccccgca accggagaaa accattcagg tttcgatggt tcagggcgat
     6361 attccgcaat cgctgaaatg ggacgaaggc cagcttctta atacgctaaa gatttactac
     6421 aacgcaacgg caccgctgat gggcaaatca tcgttgatta tctggccgga gtcggcgata
     6481 accgatctgg aaattaatca gcaaccgttc ctcaaagcac tggacggtga gttgcgtgat
     6541 aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc tcaataagca gaaccgctac
     6601 gatacctaca acaccatcat cacgctgggt aaaggtgcac cgtacagcta cgaatcagcc
     6661 gatcgctata acaaaaacca tctggtgccg tttggcgagt ttgtcccgct ggagtcgatt
     6721 ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg tgggccatat
     6781 atccagccgc cgctgtcggc aaatggtatt gagcttactg cggctatttg ctacgagatc
     6841 attctcgccg agcaagtgcg cgataacttc cgcccggata ccgactacct gctgactatc
     6901 tccaacgatg cgtggtttgg taaatctatt ggtccatggc aacacttcca gatggcgcga
     6961 atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca ccaacaacgg cattacggcg
     7021 gtgattggcc cgcagggtga gattcaggcg atgatcccgc agttcgcccg cgaggtgtta
     7081 accactaacg tgacgccgac caccggactc acaccatacg cacgtaccgg caactggccg
     7141 ctgtgggtgc tgacggcact gtttggtttt gctgctgtgt tgatgagtct gcgtcagcga
     7201 cgtaaataat cccttcttat cgtgccggaa tacatcccat atttcggcac gctcaccgga
     7261 tgcggcatca gtgcattatc cggataacaa tattctccta tccaactttg gcacatctat
     7321 tgctttgtta tacaaggcaa agcttgaacc agcatcagta caacccggtt gcaccacagc
     7381 aatccaccgg tagcaccaaa agagtgcatt ttaagaattt tgcttcagaa tggtgcggtg
     7441 cacctcgcaa aaacaaacaa taaaacatca atatctttac atttagccaa actaaatgtt
     7501 acctaacagg cacaacactg cacaataaag ttgcagacga taacaacaca aacactcaca
     7561 acgggtatcc atgcgttctt aacgcagaag ataaaggtgt tggatatgca attacgtaaa
     7621 cctgccacag caatcctcgc cctggcgctt tccgcaggac tggcacaggc agatgacgcc
     7681 gccccggcag caggcagtac gctggacaaa atcgccaaaa acggtgtgat tgtcgtcggt
     7741 caccgtgaat cttcagtacc tttctcttat tacgacaatc agcaaaaagt ggtgggttac
     7801 tcgcaggatt actccaacgc cattgttgaa gcagtgaaaa agaaactcaa caaaccggac
     7861 ttgcaggtaa aactgattcc gattacctca caaaaccgta ttccactgct gcaaaacggc
     7921 actttcgatt ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa acaggcggct
     7981 ttctctgaca ctattttcgt ggtcggtacg cgcctgttga ccaaaaaggg cggcgatatc
     8041 aaagattttg ccgacctgaa aggcaaagcc gtagtcgtca cttccggcac tacctctgaa
     8101 gttttgctca acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa
     8161 gatcacggtg actctttccg caccctggaa agcggtcgtg ccgttgcctt tatgatggat
     8221 gacgctctgc tggccggtga acgtgcgaaa gcgaagaaac cagacaactg ggatatcgtc
     8281 ggcaagccgc agtctcagga agcctacggt tgcatgttgc gtaaagacga tccgcagttc
     8341 aaaaagctga tggatgacac tatcgctcag gtgcagacct ccggtgaagc ggaaaaatgg
     8401 tttgataaat ggttcaaaaa tccaattccg ccgaaaaacc tgaacatgaa tttcgaactg
     8461 tcagacgaaa tgaaagcact gttcaaagaa ccgaatgaca aggcactgaa ctaattacaa
     8521 gaaccagggg cggaaattcc agccctctcg attgttacgt agcacggaca gactatacgc
     8581 ctgatggtcg ttccccatcg ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg
     8641 gtagcagtta acgctgctac cctctttttt ctggagtaga tttatgtcta tagactggaa
     8701 ctggggtatt tttttacaac aagccccgtt cggcaatacc acctatcttg gttgggtctg
     8761 gagtggtttt caggtgacga tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt
     8821 cggttcattt ttcggcattt tacgtaccgt tccaaaccgt tttctctccg gtctgggtac
     8881 gttgtatgtc gaactgttcc gcaacgtgcc gttgattgtg caattcttta cctggtatct
     8941 ggtgatcccg gagctgctgc cggagaaaat cggcatgtgg tttaaggccg agctggatcc
     9001 caatattcag tttttccttt catccatgct ctgcctgggg ctgtttaccg ccgcccgtgt
     9061 ttgcgaacag gtacgcgccg ctattcagtc gctaccgcgt gggcagaaga atgccgcgct
     9121 ggcgatgggc ctgacgctac cgcagactta tcgatacgta ctgctgccta atgcttatcg
     9181 cgttatcgtc ccgccgatga cttccgagat gatgaacctg gtgaaaaact ccgccatcgc
     9241 ctcgaccatc ggtctggtgg atatggcggc gcaggcgggt aaattgctcg actactcggc
     9301 tcacgcctgg gagtcgttta ccgccatcac gcttgcgtat gtgttgatta acgcttttat
     9361 catgctggtg atgacgctgg ttgaacgtaa agttcgcctg cccggcaaca tggggggcaa
     9421 ataatgtacg agtttgactg gagttccatt gttccttccc tgccatatct gctcgacggg
     9481 ctggtgatca ccctgaaaat caccgtcacg gcagtcgtga ttggtatttt gtggggtacc
     9541 atgctggcgg tgatgcgctt gtccagcttc gcgcctgttt cgtggtttgc caaagcctac
     9601 gttaacgtat tccgctcgat tcctttagtc atggttttgc tgtggtttta cttgattgtg
     9661 ccgggatttc tgcaaaatgt gctgggatta tcgccaaaaa atgatattcg tttaatttcg
     9721 gcgatggtgg cgttttcgat gtttgaagcg gcctactatt cagagattat ccgtgccggt
     9781 attcaaagta tttcccgtgg tcagtcgagc gccgcgctgg cgttggggat gactcactgg
     9841 cagtcgatga aactgattat tctgccgcag gctttccgcg cgatggtgcc gctgctgctc
     9901 actcagggca tcgtactgtt ccaggatacc tcgctggtgt atgtgttatc tctggccgat
     9961 ttcttccgta ccgcctcaac cattggtgag cgtgatggta cgcaggtcga gatgatcctg
    10021 tttgccggat ttgtttattt cgttattagc cttagcgcgt cgttgttggt cagctacttg
    10081 aaaagaagga cagcataatg attaccctga aaaatgtttc aaaatggtat ggtcactttc
    10141 aggtgctgac cgactgctca accgaagtga aaaaaggcga agtggtggtg gtttgcggcc
    10201 cgtctggttc cggcaaatca acgctgatta aaaccgtcaa cggcctcgaa ccagtgcagc
    10261 aaggtgaaat caccgtcgat ggtatcgtgg ttaacgacaa gaaaaccgat ctggcaaagc
    10321 tgcgctcccg cgtcgggatg gtattccagc atttcgagct gttccctcat ctgtcgatta
    10381 tcgaaaacct gaccctggcg caggtgaaag tgcttaaacg cgataaagcg cccgctcgtg
    10441 aaaaagcatt gaaattgctg gagcgcgtgg gactttccgc acatgccaat aaatttccgg
    10501 cgcagctttc cggcggtcag cagcagcgcg tggcaatcgc tcgcgcattg tgtatggctc
    10561 ctattgcgat gctgttcgac gaaccgacat cggcgctgga tccggagatg atcaacgaag
    10621 tgctggacgt gatggtggaa ctggcgaacg aaggaatgac catgatggtg gtgacccacg
    10681 aaatgggctt tgcccgcaaa gtggcgaatc gggtgatctt tatggacgag ggtaaaattg
    10741 tcgaagactc gccgaaagac gctttcttcg atgatccgaa atcggaccgc gcaaaagact
    10801 tcctcgcgaa aatcctgcat taatcactct ggcgcacata cccaattgcg cgccatctca
    10861 cgctttatcg tttcgcccct cgtatctgtt acatcctggc gttacccttg tcgcaaagaa
    10921 gcacacaaca aggagcaaca atggcactgc caattctgtt agattgcgac ccaggtcatg
    10981 atgacgctat cgcaatagtt ctcgcactcg cctcaccaga gcttgatgtc aaagcaatta
    11041 cgtcttccgc cggaaaccag acaccagaaa aaaccttacg caatgttctg cgtatgctga
    11101 ccttgcttaa tcgcaccgat attccggtgg caggcggcgc ggtaaaaccg ttaatgcgtg
    11161 agttgattat cgcggacaat gtgcacggcg aaagcggtct cgacggcccg gcattaccgg
    11221 aaccgacgtt cgcaccgcaa aactgtacgg cggtggagct gatggcgaaa acgctgcgcg
    11281 aaagtgcgga acctgtcacg attgtttcta ccggaccgca aactaacgtt gccttgctgc
    11341 tcaatagcca tccggaactg catagcaaaa ttgcccgtat cgtgattatg ggcggcgcaa
    11401 tggggcttgg taactggacg cctgcggctg aatttaatat ttacgttgac ccggaagcgg
    11461 cagaaattgt cttccagtca gggatcccgg tggtgatggc cggtctggat gttactcata
    11521 aagcgcaaat ccacgttgaa gacaccgagc gtttccgcgc gattggcaac cctgtttcaa
    11581 ccattgttgc cgaactgctg gatttcttcc tcgaatatca taaagacgaa aaatggggct
    11641 ttgtcggcgc accgctgcat gacccatgca ccatcgcctg gctgttgaaa cccgagttgt
    11701 ttaccactgt tgagcgctgg gttggcgtgg aaacacaggg gaaatatacc cagggtatga
    11761 cggttgttga ttattattat ctgactggca ataaaccgaa tgccactgta attgtcgatg
    11821 ttgatcgtca gggctttgtt gatttactgg ctgatcgtct gaaattttac gcttaacact
    11881 cgtcattgat taagatcacc acgcgttacc tgggtggtct taatctattt atctcttttt
    11941 ctttcaagat ataaatgcaa tggataatgc agaactcgcc ataggtatcg atctcggcac
    12001 taccaatagt ttaattgccg tctggaaaga cggtgccgcg caattaattc caaataagtt
    12061 cggtgaatat ttaacaccat ccataattag catggatgaa aataatcata ttttagtcgg
    12121 aaaaccggct gtatcacggc gtacttcgca tccggataaa acggcagcgt tatttaaacg
    12181 tgcaatgggc agctggcggt taggcagcga cacatttaac gcgccagaac tgtcctcttt
    12241 ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga ttaaagatgt
    12301 ggtgatctcc gttccggctt atttcagtga tgaacaacgc aagcataccc gtttagcagc
    12361 ggacttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag ctgcgatggc
    12421 gtatggcctg catacccaac aaaatacccg ttcgctggtg tttgatctcg gtggcggcac
    12481 gtttgacgtt acggtgcttg agtacgccac gccggtgatt gaagttcacg cctccgctgg
    12541 cgacaacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg ttttaaagcg
    12601 cgcggatgtc acaaggacca cgcttaacga gagtgaactg gcagccttgt acgcctgtgt
    12661 ggaagcggca aaatgtagca atcaatcgcc aatgcacatt cgctggtagt accaggatga
    12721 aacgcgggaa tgcgaatttt acgagaacga actggaagat ttgtggttgc cgctgctcaa
    12781 tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgcg cgtctgaagc cgagtcaaat
    12841 cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt
    12901 gcgtctgttt ggcaaattac cgtatcaaag ttacgatccg agcaccattg tcgcgctggg
    12961 cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagaag tgatcctcac
    13021 tgatatttgc ccttactcgt tgggcgttga ggttaaccgc cagggcgttt ccggcatttt
    13081 ctcgccgatt attgaacgaa acaccactgt gcccgtgtcg cgtgtagaaa cttattcaac
    13141 catgcacccg gaacaggatt caattacggt taacgtctat cagggagaaa accacaaagt
    13201 taaaaacaac attctggtgg aatccttcga tgtgccgttg aagaaaaccg gggcttatca
    13261 gtcgattgat attcgcttta gttatgatat caacgggttg cttgaagttg acgtgcttct
    13321 ggaagacggc agcgttaagt ccagagtgat taaccacagc ccggtaacat tgagcgcgca
    13381 gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc gcgatatgct
    13441 catcaatcgc acctttaaag ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga
    13501 gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgctgcagt caaacgatat
    13561 ggcccgcgtc gatgaggtca ggcggcgggc gagcgtttat ttagccattg agacctcata
    13621 atcacggctg ccggatgcgg cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg
    13681 tcaactaaac aacatcgcga ttttgccgag agcataacct ataaccagca ccgttaaact
    13741 gattttgata atgctggtaa tgctctgtgg tttttcaccc tcatcatttt tttcttcatg
    13801 gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttta ctttcgtcaa
    13861 cagcatccgg gtgataatgg cctctaatgg atgagcatct ccgccttcca gccttgtatt
    13921 aagacgggcc agcatctctt gtttcggaaa cgcttcaagt actacctgtt ccgctacccg
    13981 atacaaatcc agtaacggtg aatcttcttc tgtctcgcta ccgactctcg atttccacca
    14041 cgcccatgct ttgcgtttga agaataacga ctcaataaac gattgaccta acggcgataa
    14101 ttttccgcta tgtaaaattt ccgcccataa cagccgcgtc cgggcgctct caccaggaac
    14161 atatttcagc ggttgacctt gcgcatctac catgctccgc gcttcgataa ccatcactaa
    14221 taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat tatcagcttg
    14281 tgtagatggg taactttccc agtacgcgcc gagatcagca agcaggcttt ccccctcgcc
    14341 gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg gttcttccgc
    14401 ttgccactgt ttggctacag gaatgagttc caccatacct gtttgcaccg agctgtacca
    14461 gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc cttccatcgc
    14521 caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg cgaagtaata
    14581 ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga aatcgaaaca
    14641 atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcca ttccttcgct
    14701 gtcattaaac gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat ttaatgacag
    14761 atatcgcgct tctatagcga ttgcacacag cggccagcgt aattgatcga catcctccat
    14821 gttccaggta tttaattgct gaataaattt ctgccaggcg gagggctgaa aacgatcact
    14881 ttctgaatcc agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg tggcatcttt
    14941 ttcttcatca tctgcttcct cgacaggatt taccgccagt cgcagcgcct cttcataggc
    15001 ctggcgtaat gctttaaacc cctgcggatc ggtttcggga tgacacaacg gtaagcgagc
    15061 aagataagcc tggcggataa tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca
    15121 acatgttttc ataggtcgct cacatcctta agcgttaccg ggcattcagg ctggacagtt
    15181 aatggctgcc agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag ctcttcgaac
    15241 atccgactcc cggcgctatc gtatttgtac agggttagcg cctcgcgtag cgaagcgttg
    15301 tcttcgcact gagaagattg ccagtagaga cgccctagtg aataggcaga aaaatagtgc
    15361 atccagctgt gatagtaatg gtgagctcgt gcatagacgc gcgattgcag ccataaactc
    15421 tcttcttccg tcagccagtt gttgagcaca ccggcccgtg acagaaaccc cattcgaaca
    15481 taatcccagg ctttgattcc cccttctcca cagcacatag cggtttgggc aacgaattgg
    15541 gtataaatca accctctctc cgagccacca gcaattaatg ctttccactc ttcaggggaa
    15601 gagcgttgcc atgtcagata taagccagct aaatcattgg catgaccatc atcagtcatc
    15661 tgataaatag tctgaattaa atcatcacga ttatttattc cccaggatga tgagagatcg
    15721 aattctttta tcccggtata catttttggc gagtcatgcg cgggtttgca actctgatta
    15781 agtatttcca tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt attcattttt
    15841 taataatccc ttaagtgcat taactgacta cttttttatt attgatgagt ataaacttga
    15901 cgctttcaaa ataaaaagaa aatcgaagca ttcacacatg aataaaaaat taatgtatat
    15961 attcgcaatt tttatagttg cagcaattac ctgtattagc caacccaaga aaacgatgtt
    16021 gcgtgataaa gccatggtga attatgcctt tgattattta agatcaccag gcagtctacc
    16081 gttaaccacg gcagccacgg agctttccgc gattcatggt cactcaacat cgcaatatcg
    16141 ccttggcgaa ttttatcttc atggtagcga cggtaaacca ctggattata cacaggcgag
    16201 atactggtat gagcaatcag cagaacagga aaatccgcgc gcacaaagca aactgaggtg
    16261 gatctacctg aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc tctggtataa
    16321 ggaagcagct gaacaagggt atgctcatgc tcaatatact ttgggtttga tctacagaaa
    16381 tggcactggt attaatgtta accattatga atctcaaaaa tggttaaaac tggccgccaa
    16441 acaacattac aaaaatgcgg aaagattact tgccggactt cccgcacatt aacgtcgact
    16501 gtcacccgaa gagatggagt attttcccta tcaaaccggc aaagataaaa atgtagaaaa
    16561 taattttgat gacgcccaga cagcccagcc cgccatcatc aacgggcatt ggctgtggtt
    16621 gcggctccgg ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt aataatcgac
    16681 gaataattaa tgcttccagt ggacaacgat cttctgcatc aaggcggctc acaatcggca
    16741 ttaatgattc ttctgtcggt tggctggcct gcactaattg tttggcaact aaatataatg
    16801 gccataaggg atgttctgta tcacgcgtga gtgagatccg cggcttacgt ccttcgtgca
    16861 aaaccatctc gaccagtgaa cgccccagcg cagagagttt ttcatcgctg agtatttcgt
    16921 ataaatgaaa acgcgtacgg gcgctgtcgc cggggacata aagtagcggt tttccctgat
    16981 cgttaaccag atcacgcgct tcaatcatca tcaccactaa tgggtaataa tagacccgat
    17041 gctgacggca ccattgcaac attaaagcat ccgcctgggt ggagggatac tcgcgccagt
    17101 agtaacaaag ttctgccagc aggctttcgc cttctccaca atagacgcgt tgtgcgtatt
    17161 cataataacg cgcatcttca ttgtcaggtt cagcttcccg ccacgaccgg gcgtaatcca
    17221 ggagttcggg gattcccagg cggcttgcgc tataccagcg gagtaaattt ctgtgcagtt
    17281 ttgcatcatc ggggataatc actgcgcgat gttgcgctaa atacaatgtc agccattctg
    17341 gatgcgatga ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg
    17401 caactggcag atgcagaatg gtctggaagt taaaaacatt ccccttcgca atggcatata
    17461 aaaaggattc cagttcttct tcgtctattt ccccgacgtt ttcctcctgc aaccaccgca
    17521 atctttctgc taataacacc acgcactcga aggataaatg ggcagtgttc atggcgattg
    17581 tgcacagcga ccagcgtaat tcatcaatct cttccatcga gcaataattt aattgctgaa
    17641 tgaatcgctg ccaggcggag gacagaaaac gttcgctatc agaggcaagt aacgcgcgaa
    17701 aggcgagcag aatttcatgt tctgctacct catattcttc tggttgccaa acagatttag
    17761 caggcgactg cgcaatccgt agcgcctcct catacgcctg acgaagttgt ttaaaaccct
    17821 gcggatcggt ttctggatga aaggacggta acagcgccag ataagcgcgg cggataatat
    17881 cgacgtcagt cgtttcctct atatcgagga tcttccagca atttttcata agtcgctcat
    17941 atccttaagc gtttccgggc atgtggggta gtcagccagt ggttgccagg acaaggtagg
    18001 gtaatacgca tcatcactgg caaataattg ataaaacata tcattgccgg aatcgtcgta
    18061 ttctttacgc gctaagaatt cgcgtaataa aggcagatga tcatcttcgg gagactgcca
    18121 atattgccga ccaaaggtat aaccggcaaa gtattgccgc cagttactat aataacgtag
    18181 agcccgtaga tgaatacgcg actggatcca tagactttct tcttcactca accagttatt
    18241 aagtacgccc attcgactta aaaaacccat ccggacataa tcccatgctt taattcctcc
    18301 ctcaccacag cattcggctg tgctggcgac aaattgcgca taagcctgac cttgctcatt
    18361 taattcagca agatagtcac gccattcaca tggagaataa cgaaaccagc ggtgataaaa
    18421 ccctgccaac cgagcggcat gaccgttctc gatcattcgg tgaattatgc gcagcaggtc
    18481 atcacggtca tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac ctaaatacat
    18541 tttgggggat gaatgcgaag gtaaacagca ttcattgcgt atttccatcg gagtggaaag
    18601 tgcaaacaat attttttgcg accccatgtc catataattt atccttaaac attcatttaa
    18661 tgaatgtaaa ctacatttcg aggctcagtt atttatactt ttctgacgca acataatacg
    18721 cataaaataa ggcttacgca aaaatggaat acataaaaat ataatatatt ttatctacaa
    18781 ccataaggaa atgataatga tttttacgtc aagttgctgc gataatttat caatagatga
    18841 gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta
    18901 taatcgcgat agcgcaattg actctccgga cgacgaaaag gccttttact ggctgaagct
    18961 ggccgctgag caaggtcatt gtgaagcaca gtattcctta gggcaaaagt ataccgagga
    19021 taaaagccgt cataaagata atgagcaagc catcttctgg ctgaaaaaag ctgccctaca
    19081 aggccatact ttcgcttcca acgcccttgg ctggatactg gatcgtggag aagaccccaa
    19141 ctataaagaa gcggttacct ggtatcagat agccgcggag agcggaatgt cttatgcgca
    19201 aaataatctt ggatggatgt acagaaatgg caacggagtc gcacaagact atacgctggc
    19261 atttttctgg tacaaacaag ctgcattaca aggccatagt tacgcacaag acaatctggc
    19321 cgatctttat aaagacggag aaggcgttgc tcaaaacaag acactcgccg cattctggta
    19381 tttgaaaagc gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa
    19441 cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc
    19501 cgctcaggaa agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg
    19561 cgttgagaaa gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga
    19621 cgccactgcc tggtataacc tggccattat gtatcactac ggagaaggaa gacctgtcga
    19681 tctccgacag gctctcgacc tgtatcgtaa agttcagtca tccggaacca gagatgtcag
    19741 tcaggaaatt cgtgagactg aagctttact gtagaaataa aaacaagggc cggggaattt
    19801 cattctgccc atattctctt tattgctgtg cgaaatcaga ctattacggc tcaaacgggc
    19861 gtctctggaa ctggtcataa ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga
    19921 tcgggagatg gaagtgacat ttctcgcaga ccagatttcc cagcccgacc acttctccgc
    19981 tgtgataaac cccatgatga ttgaggtcct ggaaaatttc gcgccattca agctgcgttt
    20041 tatcggtgat gtctgccagc tcctgccaca agctttcttt aatcacccgc ataaagacgc
    20101 tgtcagattc ttctttcagg ctctcttcat agctcatggc gaactcttcc aggtcacgtc
    20161 tgacagctcg cgtcagctca tcgatctcgg ttcgcgttaa ctcccctgtt tttattacgc
    20221 gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc gccattgcgc aggcgttcgt
    20281 tcagtgacgc gaccagttca cggtaatatt gagcaacctt gttcatcgtt tcatctcccg
    20341 ggtcgttaac tgactataat aatagacgtt ctttgttcct tgctttgcta atacgcccac
    20401 aaactctgta aattaatgta cgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa
    20461 ggctgttttg acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc
    20521 tacatttgta gccgtattga aaacaggacc actggctgcc atgcaagagc aataccgccc
    20581 ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac
    20641 cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg
    20701 actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc gttaccagcg
    20761 catgctgggc aaaaacgttc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga
    20821 aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta
    20881 tatgaaaaac cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac
    20941 ctgtacgccg gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg
    21001 cctggtatat aaaaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc
    21061 gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga
    21121 gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga
    21181 taaactggat cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc
    21241 cgaaggcgtg gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac
    21301 tacccgcccg gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct
    21361 ggcgcagaaa gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa
    21421 caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg atactggctt
    21481 taaagctgtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt
    21541 gatggagtac ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga
    21601 gtttgcctct aaatacggcc tgaacatcaa accggttatc ctggcggctg acggctctga
    21661 gccagatctt tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt
    21721 caacggtctt gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg
    21781 cgttggcgag cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc gccagcgtta
    21841 ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc caaccccgga
    21901 cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat
    21961 taaagcagat ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac
    22021 cgacactttc gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccaga
    22081 gtacaaagaa ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta
    22141 cattggtggt atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact
    22201 gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg
    22261 tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc
    22321 cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc
    22381 ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg
    22441 tatcgacccg caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat
    22501 gtttgcttct ccggctgata tgactctcga atggcaggaa tccggtgtgg aaggggctaa
    22561 ccgcttcctg aaacgtgtct ggaaactggt gtacgagcac acagcaaaag gtgatgttgc
    22621 ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa
    22681 aactatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc
    22741 ggcgattatg gagctgatga acaaactggc gaaagcaccg accgatggcg agcaggatcg
    22801 cgctctgatg caggaagcac tgcaggccgt tgtccgtatg ctcaacccgt tcaccccgca
    22861 catctgcttc acgctgtggc aggaactcaa aggcgaaggc gatatcgaca acgcgccgtg
    22921 gccggttgct gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa
    22981 cggtaaagtc cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga
    23041 acgtgctggc caggaacatc tggtagcaaa atatcttgat ggcgttactg tacgtaaagt
    23101 gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg
    23161 cgatatctgg caacactgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg
    23221 catctgcgtg acaccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc
    23281 gatccgaacg ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag
    23341 ttgcttgata aagaaaccac gcgtaaggac gttccatccc tgcgtttggg taaagtgagc
    23401 atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc
    23461 atgacggtga atgcgaccgt gttgatcccc ggccgtgata tctacccgat tagcgccaaa
    23521 gtcttccgtt cattcttcga taacccgcaa atggcgttag cgaaagataa cgaacaagac
    23581 atgatcgtaa aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc
    23641 atccgtgctg cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg
    23701 gcaacgcctg cacgcgtctc caccatgctg ggtaactgat gattcggttg tacccggaac
    23761 aactccgcgc gcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc
    23821 ctctgttatt gcaggaaagc caggacgctg ttcgccaggt agctgcggca caaggattcg
    23881 aagaacacca cactttttcc attgatccca acactgactg gaatgcgatc ttttcgttat
    23941 gccaggctat gagtctgttt gccagtcgac aaacgctatt gctgttgtta ccagaaaacg
    24001 gaccgaatgc ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc
    24061 tgctattgat cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta
    24121 ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac accggagcag gctcagcttc
    24181 cccgctgggt tgctgtgcgc gcaaaacagc tcaacttaga actggatgac gcggcgaatc
    24241 aggtgctctg ctactgttat gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt
    24301 tatcgctgct ctggccagac ggcaaactga cattaccgcg cgttgaacag gcggtgaatg
    24361 atgccgcgca tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc
    24421 gcgcgttgca tattcttcag caactgcgtc tggaaggcag cgagccggtt attttgttgc
    24481 gcacattaca acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatacgc
    24541 cactgcgtgc gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg
    24601 aggcgttaaa tcgcttaagt cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa
    24661 cggaactcac cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat
    24721 ctcttctgtt gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct
    24781 ttacaggctc tgtttggcgg cacctttgat ccggtgcact atggtcatct aaaacccgtg
    24841 gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct
    24901 ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaactggcg
    24961 attgccgaca agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct
    25021 tacactgcgc aaacactgaa agagtggcgg caggaacaag gaccggacgt gccgctggcg
    25081 tttattattg gtcaggattc attgctgacc tttccgacct ggtacgaata cgaaacgata
    25141 ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa
    25201 ccgcaatacc agcaatggct ggaagatcat ttgacacata acccggaaga tcttcacctt
    25261 cagcctgccg gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc
    25321 atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtattg
    25381 acttacatta accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc
    25441 gactgtggtt aattcgtcat ggcgaaacac aagcgaatgt cgatggtctg tacagcggtc
    25501 atgcgcccac acccctgacc gcacgcggta ttgagcaagc gcaaaatctg catacactgc
    25561 tagatgatgt ttcctttgac ctggttttat gcagtgaact ggaacgggca cagcataccg
    25621 cgcgactggt tctcagtgac cgccagctcc ccgttcatat catccctgaa ctcaacgaaa
    25681 tgttttttgg cgactgggag atgcgacatc atcgcgacct catgcaagaa gatgccgaaa
    25741 actatagcgc gtggtgcaat gactggcagc atgctatccc cacgaatggt gaaggatttc
    25801 aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct tagtgaatat cagcactatc
    25861 agaatatttt aatcgtcagc catcagggcg tactgagtct gttaattgct cgtttaattg
    25921 gcatgcctgc cgagtctatg tggcattttc gcgttgacca ggggtgttgg agcgccattg
    25981 atatcaacca aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga
    26041 acgcatgact tttctgtttt tttacgggta agccgcaacg accattgaca gccccgggca
    26101 ggctgatatt ctccgcagcc agactttttc cgccagacac gactttgtag aaattgtttt
    26161 acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg
    26221 attcggcgac aattgtcccg aaatcgcctc tggttcaggt atactgacag accattttta
    26281 tctatttgat tcacccaggg ggaaaacttg cagggtaaag cactccagga ttttgttatc
    26341 gacaaaattg atgacctcaa aggtcaggac atcatcgcct tagacgttca aggcaaatcc
    26401 agcatcaccg actgcatgat catctgtacg ggtacgtcca gccgtcatgt tatgtccatt
    26461 gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt
    26521 gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag
    26581 gaagagagcc gtcgcctgta tgaactggaa aaactctgga gttaatgcgt gaagctgcaa
    26641 cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa ccggttttac cgagtacctg
    26701 cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa ttccggccgg caaacgcggc
    26761 aagaatgcgg acatcaagcg catactcgac aaagagggtg agcagatgtt ggcggccgca
    26821 ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc cctgggatac gccgcagtta
    26881 gccgctgagc tggaacgctg gaagctggat ggtcgcgacg taagtctact gattggcggg
    26941 cctgaagggt tgtcgcctgc ctgtaaagcg gcggctgagc agagctggtc gctgtcggcg
    27001 cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg
    27061 agcatcacca ccaaccatcc ttatcaccgt gagtgataag ggagctttga gtagaaaacg
    27121 cagcggatga aactacagaa ctcttttcgc gactatacgg cagagtccgc gctgtttgtg
    27181 cgccgggcgc tggtcgcctt tttggggatt ttgctgctga ccggcgtgct tatcgccaac
    27241 ctgtataatc tgcaaattgt tcgctttacc gactaccaga cccgctctaa tgaaaaccgc
    27301 attaagctgg tgcctatcgc gcccagccgc ggcattatct acgaccgtaa cggtatccct
    27361 ctggccctca accgcactat ctaccagata gaaatgatgc cggagaaagt cgataacgtg
    27421 cagcaaacgc tggacgcttt gcgcagcgtg gtagatctga ccgatgacga tattgctgca
    27481 ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa aactaaccta
    27541 accgaagtac aagtagctcg ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt
    27601 aaaggctata aacgtcgtta ctatccttac ggttcggcgt tgacccacgt catcggctat
    27661 gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata atgacggcaa actggccaac
    27721 tatgcggcaa cgcatgatat tggtaagctg ggcattgagc gttactatga agacgtgctg
    27781 cacggtcaga ccggttatga agaggttgaa gttaacaacc gtgggcgtgt tatccgccag
    27841 ttaaaagaag taccaccgca agccggacac gatatttacc tgacgctgga tctcaaactc
    27901 cagcaatata ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt taccgaaccg
    27961 cgtacaggtg gggtgctggc gctggtttcc acgcctagtt atgacccaaa cctgtttgtt
    28021 gacggtatct ccagcaaaga ttattccgcc ttgctgaacg acccgaatac accgctggtg
    28081 aaccgcgcca cacagggggt ttatcctccc gcgtctacag ttaaacccta tgtggcggtt
    28141 tcggcattaa gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg
    28201 caactaccag ggtcggaaaa acgttatcgt gactggaaaa aatggggcca cgggcgtctg
    28261 aatgtcacaa gatcgctgga agaatctgcg gataccttct tctatcaggt ggcctacgat
    28321 atggggatcg atcgcctctc cgaatggatg ggtaaattcg gttatggtca ttacaccggt
    28381 atcgacctgg cggaagaacg ttccggcaac atgcctaccc gcgaatggaa acagaaacgc
    28441 tttaaaaaac cgtggtatca gggtgacacc attccggttg gtatcggcca gggttactgg
    28501 acagcgaccc caatccagat gagtaaggca ctgatgatcc tgattaatga cggtatcgtg
    28561 aaggttccgc atttgctgat gagcaccgcc gaagacggca aacaggtgcc atgggtacaa
    28621 ccgcatgaac cgcccgtcgg cgatattcat tccggttact gggagctggc gaaagacggt
    28681 atgtacggtg ttgctaaccg ccctaatggt acggcgaata aatactttgc cagcgcaccg
    28741 tacaaaattg cggcgaaatc tggtaccgct caggtcttcg gtctgaaagc gaacgaaacc
    28801 tataatgcgc acaaaattgc cgagcgttta cgtgaccaca aactgatgac cgcctttgcg
    28861 ccatacaaca atccgcaagt ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg
    28921 gcggttggta cgctgatgcg ccagatcctc gaccacatta tgctgggtga taacaacacc
    28981 gatctgcctg cggaaaatcc agcggttgcc gcagcggagg accattaatc atgacggata
    29041 atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg ctgctgattt
    29101 tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt caggatattg
    29161 gcatgatgga gcgtaaaatc ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg
    29221 cgcaaattcc tccacgcgtt tatgaaggct gggcccccta tctctatatc atctgtatta
    29281 ttttgctggt ggcggtagat gctttcggag ccatctctaa aggcgctcaa cgctggctgg
    29341 acctcggtat tgttcgtttt cagccgtcgg aaattgccaa aatagccgta ccactgatgg
    29401 ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaatact ggcatcgcgc
    29461 tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg ggaacatcaa
    29521 tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc tggcgtctga
    29581 ttggcgtcgc agtagtgctg gtagcggcgt tcattccgat tctgtggttc ttcctgatgc
    29641 atgattacca gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac ccactcggcg
    29701 cgggctatca cattattcag tctaaaattg ctattggctc cggcggatta cgcggcaaag
    29761 gctggctgca cggcactcag tcacagcttg aatttctccc cgaacgccat actgacttta
    29821 tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg ctcgctctct
    29881 acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc acctttggtc
    29941 gcgtcatggc tggtggctta atgctgatat tattcgttta tgtcttcgta aatattggta
    30001 tggtaagcgg tattctgccg gttgtagggg ttccgctccc actggtcagt tatggaggat
    30061 cggcgctaat tgtgctgatg gctgggttcg ggattgtaat gtcaatccac acccacagga
    30121 aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct cgggatctgc
    30181 atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt
    30241 gtaccgcagc ctgcggtatg taacggcccc atagttgaaa ttagcggggc ggacccgcgt
    30301 ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa aagctacaaa
    30361 atcgtgcagg atccgtctcg atttagccag gcgggactgg cggcaatcta tgatgccgaa
    30421 ccaggcagta acctgacggc ctctggcgaa gctttcgatc cgacaaagct gacggcggcc
    30481 catccaacgc ttccgatccc cagctacgcc agaatcacta acctggctaa cgggcgaatg
    30541 atcgtggtgc gcattaatga tcgcggccct tacggcaacg accgcgttat ttcgctttct
    30601 cgcgcggcag ctgaccgtct taacacgtca aacaacacca aagttcgtat cgatccgatt
    30661 attgtcgccc aggatggttc gctttctggt cctggtatgg cttgtaccac agtcgccaaa
    30721 cagacttacg ccctgcctgc acctcccgat ttaagcggtg gcgcgggaac aagttcagtg
    30781 tctggcccgc agggtgacat tcttccggtc agtaattcga cgctaaaaag cgaagatccg
    30841 accggcgcgc cggtaaccag tagcggtttc ctcggcgctc caacgacctt agcgcctagt
    30901 gtactggaag gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc ttcgtcgaca
    30961 acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc
    31021 aactttatgg tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca gtaccaacag
    31081 caactgggac agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg
    31141 atccagcttg gcccattcgc cagcaaagcc gaagccagta ccttgcagca acgtttgcaa
    31201 accgaagccc aattacagtc atttattact accgcgcagt agcgtaaagc aggcatctga
    31261 tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat gcctgccggt
    31321 agtgcatttg ctatagtagg gcactttttt aattccatca cggatgtcgt agttcagacc
    31381 atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc
    31441 acagccttta tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt
    31501 gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc
    31561 gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt
    31621 tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt agtcactatc
    31681 ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc
    31741 aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt
    31801 aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc
    31861 ttgatgaaca gctacgttaa cgcactgggc ctgaaaaata cccacttcca gacggtacat
    31921 ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat cggccaggca
    31981 ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac
    32041 ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc
    32101 atcaaaaccg gacacactga caaagcaggt tacaatcttg ttgcttctgc gactgaaggc
    32161 cagatgcgct tgatttctgc ggtgatgggt ggacgtactt ttaaaggccg tgaagccgaa
    32221 agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta
    32281 ggtaaagagt tcgcctctga accggtttgg tttagtgatt ctgatcgcgc ttcgttaggg
    32341 gttgataaag acgtgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc
    32401 tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact
    32461 atcaacttcc agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa
    32521 atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac
    32581 tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat actaagcatc
    32641 agtaaaaaaa ctcccgcctt ctggcgggag ttgctattta attacgttac gccggagctg
    32701 acatgaaaac caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta
    32761 tggggcaggc gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc
    32821 caggtgacta caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta
    32881 tcactatcaa cgccactcat atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa
    32941 tcgatattgt ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta
    33001 actcccctct cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat
    33061 gccgttttgt atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca
    33121 atctcccggg ctatgcatga attcaccgat acccgcgata atagtaccct tgatgaaatc
    33181 tggctggtcg agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt
    33241 ttaatgccgg gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac
    33301 gggccgggac aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg
    33361 cgtgaactgg tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata
    33421 gaagcgcatc ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca
    33481 ctgggtttac gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg
    33541 gatctttcac catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa
    33601 atatcacaat ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat
    33661 attttagcgc tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca
    33721 atggcccaat ttacactggg tcattactca taatacctgc taatgcttta catctccttc
    33781 gcgtttgtat attttcttta tcacttgcag aaatgcggtg ccacgccttg ctccgtaact
    33841 caccccattg attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca
    33901 attagacata agattattgt aattttttaa atattatttt cccatgaata aaattggagt
    33961 aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt
    34021 aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac tatttttgaa
    34081 gctgtatatg tacataaagg gatcgttaat gcagcgaaag tgcttaatct gaccccctcg
    34141 gcaatcagtc agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc
    34201 aaaggccagg gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag
    34261 ggccttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg
    34321 ataactattg cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg
    34381 attaaaactc actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac
    34441 caactcagtc agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg
    34501 gtgcaacatc atgttctgtt caccgacaat atggtgttaa tttgccgcaa gggaaatcca
    34561 ctactctctt tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta
    34621 ccggaagggc aaaatttcag cggtctgcgg cagagagttc aagagatgtt tccggaccgg
    34681 caaatcaatt tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac
    34741 atgttagcga ttattcccag ccgtttttat aacctgttta gtcgttgctg gccgctggaa
    34801 aaactgcctt ttccgtcctt aaatgaggag caaatagatt tctctatcca ctacaacaaa
    34861 ttcatcctgc gtgatcccat cctgcacgga gtaatagatg ttatccgtaa tgcattttga
    34921 aaaagtattg ttacagccag atcacagcgc agaacgataa aatggcacaa tccaacaaca
    34981 attttacatt ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga tatactgcca
    35041 gtcgttaatt caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt
    35101 cgtaattcgc aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt
    35161 taaataccgc gatgccgata agatggccct tatcccggtt aaaaacgtgg caacagagcg
    35221 cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg
    35281 tatccagggc atcaaagccg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc
    35341 ctcctgccct aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg
    35401 cgctatttgt acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc
    35461 tgatgccaat gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt
    35521 ggttatcacc tccgttgacc gtgatgacct gcgcgatggc ggtgcccaac actttgcgga
    35581 ttgtattact gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga
    35641 tttccgcggt cgaatggatc gtgctctgga tattctgact gcaacgcccc cagatgtgtt
    35701 caaccataac ctggaaaacg taccgcgtat ttaccggcag gtacggcctg gcgcagatta
    35761 caactggtcg ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa
    35821 gtctggtctg atggtgggtc tgggtgaaac caatgaagaa attattgagg taatgcgcga
    35881 cctgcgccgt catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca
    35941 tcacctgccg gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc
    36001 gctggcgatg ggctttaccc atgctgcatg cggtccgttt gtccgctctt cttaccacgc
    36061 cgatttgcag gcgaaaggaa tggaagttaa gtaatcctgt aataattact tacatctgtc
    36121 tgataaaaaa cccgcttttt gaagcgggtt ttttgtatcg aacaagatat tgagggagcg
    36181 tcctgctcgc cacgtcactc tttatgagag agcttttcag cctgaagatc aacgtctgcg
    36241 ccttttttcg ccgcagcatc gtcatcattc atcgccttct tgaacccttt aatggccgct
    36301 ccaaggtctc cgcccagcgt acgtaacttc ttagtcccaa acagcagaac gaccagcgcc
    36361 gcaactacca gcagtttggt aatactaatc tcacccatag ataccttctt gacatataac
    36421 cggcaacaga acgcgcctta aaaaccagag aatattaacg gtcattcgac gcgcgcacac
    36481 aatagcaatc tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat aataattgcg
    36541 gcggcgcaaa gcgacggttg tttaagatgg gcagttgcgc gcgcacctga cgcacacgtt
    36601 cgggcgtcac ttccgccata atgagtgcag gcatttctga cgctgccgca atggtgacgc
    36661 caaagggatc tataatccgg ctttgcccga tatttttgtt cccgcactcc cccgccgcca
    36721 ccatataaca ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag tgatgctctt
    36781 tgagcggccc acgaacccag gcggcaggaa gtaccaggat ttcagctccc tgtaaagcct
    36841 gtgccagcgc cagctctgga aagcgtaagt cataacaggt catcagaccg accttcatcc
    36901 cttccacctc cagtaacgga gctatttcat taccagcatc aacatggcgt gattcctgaa
    36961 tggcaaatgc atcatagaga tgcagtttgg cataacgggc gacgatgttt cctgcctgaa
    37021 gtgccaccag catattccat gcgcgccccg gcgttgaagg aagatgaatc gtcagaattg
    37081 tcgtcatcat gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca
    37141 gctgtgctga tttaaccgat agatctgcat catgatcatc gcgcgccagc aatgcttccg
    37201 gcaggacaaa cagcgatacg tcgttttccg ccgcctgcgc catcaacgag gcacaaatct
    37261 cagcgttctt ttcccacaca gatgtaacag caaactgtcc tgcagcaacc agcattgcca
    37321 cctcctgtca gagtcgtaaa attcgccaac gacgattatt cggcgttaca cttatcactc
    37381 atacaaatca aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttattggc
    37441 ggtggtacgg gaagcgtagc gagatggctg ttaagtatgc gatttaaccc gctgcatcag
    37501 gcgattccgt tggggacgct ggcagcaaac ctgattgggg cattcatcat aggaatggga
    37561 ttcgcctggt tcagcaggat gacgaacatt gatccagtgt ggaaagtatt aatcaccacc
    37621 ggattttgtg gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta
    37681 caagagggcc gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt
    37741 gccatgaccg cactggcatt ctggctgttt tcggcctcaa ccgtacacta aagaccaaaa
    37801 aaaacccgct gattaagcgg gttttgaatt cttgctgacg tatcttacag agcgattacg
    37861 tttgcagcag aagggccttt ggcaccgtta gtgatttcga actctacgcg ctgaccttca
    37921 gcaagagttt taaaaccatt agtctggatt gcagagaagt gtacgaacac gtctttgctg
    37981 ccgtcttccg gagtaatgaa accgaatcct ttggactcat taaaccactt aacgttacct
    38041 ttaatcttag acatcaaaat tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt
    38101 atcagtacac caattgtggt acgctttgtc cagtgcaact ttactaaaaa gtgataaaag
    38161 tcgcgttatt tttgatgttg cttattttag ttattgattt taaagaagtt actaaaactt
    38221 catttgtctc aaaactgaaa gcgcatccag gcaaagtaca cattgccatt gttgtaggta
    38281 cccggaatgt aggtcatctg aaaagtcact gggccataac ccacggaggc caatggcagt
    38341 agaaccggga gagggatgta attccagtta tcgcgtgccg ttacgccagc ggtgaatccc
    38401 agacctaaat gaaaattttc atccgccagc ggtcgccagg tactttccca tccgtatccg
    38461 gcaatcggtt cccatttgtt ccacgagtcc ttaaatgcca tggcatacag gccatgccag
    38521 tttccttttt catcccaacg cgacaggcca aaaccgccac cccacggtcg ctcgttatag
    38581 cgatcggttt tttctttgtc gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat
    38641 aaatcataat gttcaggctg ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc
    38701 ctctcatctg cgttagcaaa aactttacca acgctgatta actgaataaa aacaaaggaa
    38761 aagatagcga catatttact cacgttcatt tgtgaccata aaacatttat caaaaatcta
    38821 ctactagcat agcaaagcta caattaacat aaccttaata gacccaacat aaagaataat
    38881 ctgaatagct gctctattta tttccgcttg ttcaagttaa atatttccgc gcatcgatta
    38941 aagatgatgc gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt
    39001 gatgacaaaa tgcaatcaag gaaaaattat tttatttttt taacgagata caacaatcat
    39061 cttaacgaag tatataatat atatcgtcaa ccatgctcat attagattat tttttgagcg
    39121 cacagccgca gctaacacca ttgcaattaa caaatttgca tcaatccacc atcaatttac
    39181 acacattatt atgtgataat tgccaaccgc taaatatgcg ttttgttatc tatgtataaa
    39241 aacagcaact tcaatgtctt aatggcagtt tttcttgatt ttaatcagca ttcatcgcca
    39301 atttattggg catatttttt ccttaagctt taggaagttt ttattattta ctttgggcct
    39361 ggagacagga aaaattatgc tgacattcat tgaactcctt attggggttg tggttattgt
    39421 gggtgtggct cgctacatca ttaaagggta ttccgccact ggtgtgttat ttgtcggtgg
    39481 cctgttattg ctgattatca gtgccattat ggggcacaaa gtgttaccgt ccagccaggc
    39541 ttcaacaggc tacagcgcca cggatatcgt tgaatacgtt aaaatattac taatgagccg
    39601 cggcggcgac ctcggcatga tgattatgat gctgtgtggc tttgccgctt acatgaccca
    39661 tatcggcgcg aatgatatgg tggtcaagct ggcgtcaaaa ccattgcagt atattaactc
    39721 cccttacctg ctgatgattg ccgcctattt tgttgcttgt ctgatgtcac tggccgtctc
    39781 ttccgcaacc ggtctgggtg ttttgctgat ggcaaccctg tttccggtga tggtaaacgt
    39841 tggtatcagt cgtggcgcag cagctgccat ttgtgcctcc ccggcggcga ttattctcgc
    39901 accgacttca ggggatgtgg tgctggcggc gcaggcttcc gaaatgtcgc tgattgactt
    39961 cgccttcaaa acgacgctgc ctatctcaat tgctgcaatt atcggcatgg cgatcgccca
    40021 cttcttctgg caacgttatc tggataaaaa agagcacatc tctcatgaaa tgttagatgt
    40081 cagtgaaatc accaccactg cccccgcgtt ttatgccatt ttgccgttca cgccgatcat
    40141 cggtgtactg atttttgacg gtaaatgggg tccgcaatta cacatcatca ctattctggt
    40201 gatttgtatg ctgattgcct ccattctgga gttcctccgc agctttaata cccagaaagt
    40261 tttctctggt ctggaagtgg cttatcgcgg gatggcagat gcgtttgcta acgtggtgat
    40321 gctgctggtt gccgctgggg tattcgctca ggggcttagc accatcggct ttattcaaag
    40381 tctgatttct atcgctacct cgtttggttc ggcgagtatc atcctgatgc tggtattggt
    40441 gattctgacc atgctggcgg cagtcacgac cggttcaggc aatgcgccgt tttatgcgtt
    40501 tgttgagatg atcccgaaac tggcgcactc ttccggcatt aacccggcgt atttgactat
    40561 cccgatgctg caggcgtcaa acctggatcg taccctatca cccgtttctg gcgtagtcgt
    40621 tgcggttgcc gggatggcga aaatctcacc atttgaagtc gtaaaacgca cctcggtgcc
    40681 ggtgcttgtt gggctagtga ttgttatcgt tgctacagag ctgatggtgc caggaacggc
    40741 agcagcggtc acagacaagt aaatagtaat gccggaggca agtttcctcc ggctttatca
    40801 tgtcacccac tgtggtatat gcgttgtggt ctgccaactt tgccgtgaac aatttcagca
    40861 ataatcagat ggcggctggc gcaatattca agataacgcc tggcagtggt gcggctgatg
    40921 gtcagtgcct gcgccaccgt ttctgccgta tgttgcacac caggctcttt aaacagtttt
    40981 cgcaccgcgt ttagcgtcaa ggggtcaatg ccggtcggca gttcgtcttt gggttcaccg
    41041 cgagcatagg cattaaacat ctcatcaatt tgcttctggc tggcgctatc aatactttcc
    41101 agcatatgtt tacgctggcg gaaacgggtt agcgtttgcc ccagtgtatt gattcacttg
    41161 aagtacgaaa aaaccgggag aacattggat tattcgggat ctgatgggat tagatttggt
    41221 ggggcttgca agcctgtagt acaaatttta ggcgttaacc aatgaaacgc gaaagatagt
    41281 aaaaaattgc ttttgtttca ttgaaaaaac gaaaaacaaa aacactataa atcatttcaa
    41341 tcatagcttc aaaaaacgtt caaaaccgat aacaaccagg ctgtcaccaa atgactcata
    41401 tcacaaatca gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat
    41461 gttttagctg gctcatggtt gttatacggc ttttttcgca tcttgtggtt ccaacaaagg
    41521 agctacaggc tcttcctgtt tatgaacacc atacattgac aaaatctctt ggcgagtttc
    41581 tggaggtaat ttatgcagcg ccaaagctgt ttgtagtgtt tggcgatcaa tacccagcgt
    41641 tgccaatagg gtgtcaacgg agatggaggg gcgttcttgc attaatccgg aaagcccata
    41701 tttagccatg tttagctcta tctctcggac ttcattttct cctagtttat tgattaacag
    41761 ggagaacatt ttcaatgtat cagcattcat ttcttcgata gcaatttttt gcatagccgg
    41821 tattgccggg atatattcaa ccccaggccc gcgacgccct tcttgtgaac gcttcggcca
    41881 tccttctttc tttgctcgtt tatgcacccc ctgtattgtc ccaggcattc catccagttc
    41941 agtaatttgt ttgggtgtca tccactggtt tatctcattt gcgcttttca taaactaagt
    42001 ttaccagatt tatctatata aaaatcaatg gattacaaaa taaaccacat taactgatat
    42061 aaaccggttt tgtttatttg gtaatgtata aaacagataa tcaagacgaa ttatttgttt
    42121 tggtaaccct ttatcatacg caatatggtt ggtgtgcgaa agatgagtaa cgattggcat
    42181 cgggccgacg tgattgcggc cctcaaaaaa acaggaaccc ctctggcaaa attgtccaga
    42241 cgttcaggtt attcaaccgg gacgcttgca aatgcattag tcaggccatg gcctaaaggt
    42301 gaacgcatta ttgctgacgc cttaaacatg aaacctgagg aaatttggcc tagtagatat
    42361 ctaagggatc acaatgagag agtggatatc agcaattgaa tgcatgggtt tatctggttt
    42421 acctggttca ttaatggggg ttcacaaaag agctcagaga gaaggatggc tcaagagaag
    42481 aagagaagga attagagggg ttgcttacga ataccatata tcttctcttc ctaacattgc
    42541 tcgagctgaa gttttgctcc aacaaggaaa atttaaaacc agtcagggat gttttgaaat
    42601 cgcccgcccc acgctggaag ctcatgatta tgatcgtgag gcactatgga gcaaatggga
    42661 taaagccagc gattcccagc gcagcctggc tgaaaaatgg ttgccctcaa ttcaggccac
    42721 agacgaaatg ctgaaccagg ggatttcaac gaaaacggct tttgcgaccg ttgcagggca
    42781 ttaccaggtc agcgcatcca ctttgcggga caagtattac caggtacaga agtttgcgaa
    42841 gcctgactgg gcggctgcac ttgttgatgg acgtggagca tcccgtcgca atgttcacaa
    42901 aagtgaattt gacgaggatg cctggcagtt tctgattgca gattatctgc gaccggaaaa
    42961 acccgccttc cgcaaatgtt atgagcgtct ggaactggca gcccgcgagc atggctggag
    43021 tattccctcc cgtgccacgg cctttcgccg gattcagcaa ctggacgagg caatggttgt
    43081 tgcctgtcgt gaaggtgaac atgcactgat gcatctgata ccggcacagc agcgaactgt
    43141 ggaacacctg gacgccatgc agtggatcaa cggcgacggt tatctgcata acgtctttgt
    43201 acgctggttt aacggtgatg tgatccgtcc gaaaacatgg ttctggcagg atgtgaaaac
    43261 ccgaaaaatt ctgggctggc gctgcgatgt gagcgagaac attgattcaa ttcgcctctc
    43321 gttcatggat gttgtgactc gctacagtat cccggaggat tttcacatca ccattgataa
    43381 cacccgtggt gctgcgaata aatggctgac gggaggcgcg cccaatcgct accgctttaa
    43441 ggtaaaagag gacgatccaa aaggactgtt tttactgatg ggtgcgaaaa tgcactggac
    43501 aagcgttgtt gccggtaaag gctggggcca ggcaaaacct gttgaacgtg ctttcggtgt
    43561 tggtgggctt gaggaatacg ttgataagca tccggcactg gctggcgcat atacggggcc
    43621 aaatccgcag gcaaaacctg ataactatgg cgaccacgct gttgatgcag agctgtttct
    43681 gaaaaccttt gccgaaggtg tggcgatgtt caatgccaga acaggccgtg aaacagaaat
    43741 gtgcgggggc aaactctcgt ttgatgatgt tttcgagcgt gaatacgcca gaacgattgt
    43801 gcgtaagcca accgaagaac aaaaacggat gctgttactg cctgccgagg cggtgaacgt
    43861 ttcacgcaaa ggcgagttta cgcttaaagt tggcggctcc cttaaaggcg cgaaaaacgt
    43921 ttattacaac atggcattaa tgaatgccgg agtgaaaaaa gttgtggtca gatttgatcc
    43981 acagcagtta cacagcacgg tttattgaga tagcctgagt ttaacgga
//
LOCUS       DAJVRL010000025        43836 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVRL010000025 DAJVRL010000000
VERSION     DAJVRL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.025"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(16..603)
                     /gene="mog"
                     /locus_tag="OQN02_001334"
     CDS             complement(16..603)
                     /gene="mog"
                     /locus_tag="OQN02_001334"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1642193.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(718..1671)
                     /gene="tal"
                     /locus_tag="OQN02_001335"
     CDS             complement(718..1671)
                     /gene="tal"
                     /locus_tag="OQN02_001335"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1642194.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1950..3373
                     /locus_tag="OQN02_001336"
                     /pseudo
     CDS             1950..3373
                     /locus_tag="OQN02_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3443..4219
                     /gene="yaaA"
                     /locus_tag="OQN02_001337"
     CDS             3443..4219
                     /gene="yaaA"
                     /locus_tag="OQN02_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1642195.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4372..4668)
                     /locus_tag="OQN02_001338"
     CDS             complement(4372..4668)
                     /locus_tag="OQN02_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1642196.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4882..6168)
                     /gene="thrC"
                     /locus_tag="OQN02_001339"
     CDS             complement(4882..6168)
                     /gene="thrC"
                     /locus_tag="OQN02_001339"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1642197.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6169..7101)
                     /gene="thrB"
                     /locus_tag="OQN02_001340"
     CDS             complement(6169..7101)
                     /gene="thrB"
                     /locus_tag="OQN02_001340"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1642198.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7103..9565)
                     /gene="thrA"
                     /locus_tag="OQN02_001341"
     CDS             complement(7103..9565)
                     /gene="thrA"
                     /locus_tag="OQN02_001341"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1642199.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9591..9710)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9646..9711)
                     /gene="thrL"
                     /locus_tag="OQN02_001342"
     CDS             complement(9646..9711)
                     /gene="thrL"
                     /locus_tag="OQN02_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1642200.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9925..10611)
                     /locus_tag="OQN02_001343"
     CDS             complement(9925..10611)
                     /locus_tag="OQN02_001343"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1642201.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11011..11151)
                     /locus_tag="OQN02_001344"
     CDS             complement(11011..11151)
                     /locus_tag="OQN02_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642202.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11247..11963
                     /gene="arcA"
                     /locus_tag="OQN02_001345"
     CDS             11247..11963
                     /gene="arcA"
                     /locus_tag="OQN02_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1642203.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12022..13374)
                     /gene="creD"
                     /locus_tag="OQN02_001346"
     CDS             complement(12022..13374)
                     /gene="creD"
                     /locus_tag="OQN02_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1642204.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13432..14856)
                     /gene="creC"
                     /locus_tag="OQN02_001347"
     CDS             complement(13432..14856)
                     /gene="creC"
                     /locus_tag="OQN02_001347"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1642205.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14856..15545)
                     /gene="creB"
                     /locus_tag="OQN02_001348"
     CDS             complement(14856..15545)
                     /gene="creB"
                     /locus_tag="OQN02_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1642206.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15558..16031)
                     /gene="creA"
                     /locus_tag="OQN02_001349"
     CDS             complement(15558..16031)
                     /gene="creA"
                     /locus_tag="OQN02_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1642207.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16242..17111
                     /gene="robA"
                     /locus_tag="OQN02_001350"
     CDS             16242..17111
                     /gene="robA"
                     /locus_tag="OQN02_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1642208.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17108..17755)
                     /gene="gpmB"
                     /locus_tag="OQN02_001351"
     CDS             complement(17108..17755)
                     /gene="gpmB"
                     /locus_tag="OQN02_001351"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1642209.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17807..18327
                     /gene="yjjX"
                     /locus_tag="OQN02_001352"
                     /pseudo
     CDS             17807..18327
                     /gene="yjjX"
                     /locus_tag="OQN02_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18412..18738)
                     /gene="trpR"
                     /locus_tag="OQN02_001353"
     CDS             complement(18412..18738)
                     /gene="trpR"
                     /locus_tag="OQN02_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1642210.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18828..20765)
                     /gene="sltY"
                     /locus_tag="OQN02_001354"
     CDS             complement(18828..20765)
                     /gene="sltY"
                     /locus_tag="OQN02_001354"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1642211.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20976..22643
                     /gene="ettA"
                     /locus_tag="OQN02_001355"
     CDS             20976..22643
                     /gene="ettA"
                     /locus_tag="OQN02_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1642212.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22750..22917)
                     /locus_tag="OQN02_001356"
     CDS             complement(22750..22917)
                     /locus_tag="OQN02_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642213.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(22951..24183)
                     /gene="nadR"
                     /locus_tag="OQN02_001357"
     CDS             complement(22951..24183)
                     /gene="nadR"
                     /locus_tag="OQN02_001357"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1642214.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24204..25586)
                     /gene="radA"
                     /locus_tag="OQN02_001358"
     CDS             complement(24204..25586)
                     /gene="radA"
                     /locus_tag="OQN02_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1642215.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25635..26603)
                     /gene="serB"
                     /locus_tag="OQN02_001359"
     CDS             complement(25635..26603)
                     /gene="serB"
                     /locus_tag="OQN02_001359"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1642216.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26709..27353
                     /locus_tag="OQN02_001360"
     CDS             26709..27353
                     /locus_tag="OQN02_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1642217.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27381..28396
                     /gene="lplA"
                     /locus_tag="OQN02_001361"
                     /pseudo
     CDS             27381..28396
                     /gene="lplA"
                     /locus_tag="OQN02_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28428..28691
                     /locus_tag="OQN02_001362"
     CDS             28428..28691
                     /locus_tag="OQN02_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1642218.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28852..29571)
                     /gene="deoD"
                     /locus_tag="OQN02_001363"
     CDS             complement(28852..29571)
                     /gene="deoD"
                     /locus_tag="OQN02_001363"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1642219.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29628..30851)
                     /gene="deoB"
                     /locus_tag="OQN02_001364"
     CDS             complement(29628..30851)
                     /gene="deoB"
                     /locus_tag="OQN02_001364"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1642220.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30903..32225)
                     /gene="deoA"
                     /locus_tag="OQN02_001365"
     CDS             complement(30903..32225)
                     /gene="deoA"
                     /locus_tag="OQN02_001365"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1642221.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32352..33131)
                     /gene="deoC"
                     /locus_tag="OQN02_001366"
     CDS             complement(32352..33131)
                     /gene="deoC"
                     /locus_tag="OQN02_001366"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1642222.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33389..34930
                     /locus_tag="OQN02_001367"
     CDS             33389..34930
                     /locus_tag="OQN02_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1642223.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34902..35765
                     /locus_tag="OQN02_001368"
     CDS             34902..35765
                     /locus_tag="OQN02_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1642224.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35979..36761)
                     /locus_tag="OQN02_001369"
     CDS             complement(35979..36761)
                     /locus_tag="OQN02_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1642225.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36758..37831)
                     /locus_tag="OQN02_001370"
     CDS             complement(36758..37831)
                     /locus_tag="OQN02_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1642226.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37953..38114)
                     /locus_tag="OQN02_001371"
     CDS             complement(37953..38114)
                     /locus_tag="OQN02_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1642227.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38231..38836)
                     /gene="osmY"
                     /locus_tag="OQN02_001372"
     CDS             complement(38231..38836)
                     /gene="osmY"
                     /locus_tag="OQN02_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS1642228.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39229..40818)
                     /gene="prfC"
                     /locus_tag="OQN02_001373"
     CDS             complement(39229..40818)
                     /gene="prfC"
                     /locus_tag="OQN02_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1642229.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40909..41586)
                     /gene="yjjG"
                     /locus_tag="OQN02_001374"
     CDS             complement(40909..41586)
                     /gene="yjjG"
                     /locus_tag="OQN02_001374"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1642230.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41601..42047)
                     /gene="rimI"
                     /locus_tag="OQN02_001375"
     CDS             complement(41601..42047)
                     /gene="rimI"
                     /locus_tag="OQN02_001375"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1642231.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42016..42429)
                     /gene="holD"
                     /locus_tag="OQN02_001376"
     CDS             complement(42016..42429)
                     /gene="holD"
                     /locus_tag="OQN02_001376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1642232.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42532..43563
                     /gene="rsmC"
                     /locus_tag="OQN02_001377"
     CDS             42532..43563
                     /gene="rsmC"
                     /locus_tag="OQN02_001377"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1642233.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        10322 a        11987 c        11089 g        10438 t
ORIGIN      
        1 ctcgggggga tttttttatt cgctaacgtc gcgtcttgca ctcttcggtc tgaatgcagc
       61 aaccacttct ggtgccgttt caacgtatgg cccttccagc aactgaatgc agtacggtac
      121 gctggcaaaa ataccatgca ccacaacgtt accctcagcg tccttcacac cttccagcgt
      181 ctctttaata gacttcggct gaccgggtaa gttaaggatc agcgcctgtt tgcgaatcac
      241 gcccacctga cgcgaaagga tcgcagttgg tacaaaatgc aggctgatct ggcgcatctg
      301 ttcaccaaag ccaggcatct cgcggtccgc tactgccagc gtcgcatcgg gcgttacgtc
      361 acgacgcgcc gggccagttc cgcccgtggt gagcaccaga tggcaactca tttcatccac
      421 cagctcacac aacgtttgct cgatgatcgc ctgctcatcg gggattaagc gggtttccag
      481 ttcaaacggc gtggttagcg ccgatgtcag ccattcttcc agcgctggaa tgcctttatc
      541 ctgataaacg ccgctggatg cgcgatcaga gatggaaact aagccaatac gtaaagtatt
      601 catcaagatt tttccgttta aacaatctcg ttaaacagaa tgatacactg cgaagggagt
      661 gacagacagg cttcagaaga ggtagcgtga ccgacttccc ggtcacgcta agaatgatta
      721 cagcagatcg ccgatcattt tttccagttt ttcctggtca atagcaaact tacggatacc
      781 ttccgccagt ttatctactg ccattggatc ctggttgtgc tgccacagga actcggactc
      841 agtgatacgc gccggacgcg ctttcacttc gccggtgtaa gacagtttac gttcgatagc
      901 cccttcgctc tccgccagct ctttcagcag tgccggtgcg atggtcagac ggtcacagcc
      961 tgccagttcc agaatttcgc cgatgttacg gaagcttgcg cccataacca cggtttcata
     1021 accgtgctct ttgtagtact ggtagatttc agatacagaa accacgcccg gatcttctgc
     1081 cggagcgtac tctttcttat cggtattcgc tttgtaccag tcaagaatac ggccaacaaa
     1141 cggcgagatc aggaacacgc ccgcctccgc acaagcacga gcctgagcga aggagaacag
     1201 cagggtcagg ttacagttga taccttcttt ttccagctgt tctgcagcac ggataccctg
     1261 ccaggtagaa gccagtttga tcagaatacg atcgttgcta atacctgcat cgttgtagag
     1321 tttgatcagg cgttttgctt tcgcaattga cgcttcggtg tcataggaaa gacgcgcatc
     1381 aacttcagtt gagatacggc ccggaaccag tttcaggatt tccagaccaa tatttactgc
     1441 cagtttgtcg gtcgcgtcca cgatctgctg cgcgcgatcg ttgctctgct gtttcgccca
     1501 ggcgacagca tcatcaatca acttacggta ttccggaatc tgcgctgcgt taagaatgag
     1561 agaagggttg gttgtggcat cctgcggttg atacagcttc attgccgcga tgtccccagt
     1621 gtcggccact acggtggtgt actgacgaag ggaggtcaat ttgtccgtca tgatagtatt
     1681 tctctttaaa cagcttgtta gggggatgta accggtctgc cctgatgata tcacgacgca
     1741 acgtcagcgc aaccgccgac aagacggtta tcgccaggcg agactgtttc ggatttctga
     1801 cactgcgttg atatcgccca ccatttttat acaaaacctc atgtatgcta cgcagaagtt
     1861 atcaagtacc tcgtagcgta tatacttctt aaacaattgg taacgtttac acaggaaagt
     1921 catcgcgacc ggcaataaga gggatatgca tgccagattt tttctccttt attaacagcg
     1981 tcctttgggg atcggtaatg atttacctgc tcttcggcgc aggttgttgg ttcacttttc
     2041 gcaccggatt tgtgcagttt cgctacatcc gccagtttgg caaaagtctt aaaaatagca
     2101 ttcatccaca gccaggcggt ttaacctcat ttcagtcatt gtgtaccagt cttgcggcgc
     2161 gcgtgggtag cggcaacctg gccggcgttg cgctggcaat taccgccggt ggaccaggtg
     2221 ccgtcttttg gatgtgggtt gctgcgttta tcggcatggc gacctcgttt gccgaatgtt
     2281 cccttgcaca actttataaa gaacgtgacg tcaatgggca gtttcgtggc ggaccggcat
     2341 ggtatatggc gcgcgggctg gggatgcgct ggatgggcgt tctgttcgcc gtctttttgc
     2401 tcatcgccta cggcataatt ttcagcggag ttcaggcgaa cgccgttgcc cgagccctga
     2461 gtttttcttt tgattttcct ccgctggtga cgggcattat tctcgctgtc tttgctctgc
     2521 tggcaatcac tcgcggtctt catggcgtcg cccggctcat gcaggggttt gtcccgttga
     2581 tggcgataat ctgggtactg accagcctgg tgatttgcgt aataaatatc gggcaacttc
     2641 cccacgtcat ttggtctatt tttgagagtg cttttggctg gcaggaagcg gcaggcggcg
     2701 cggcgggata taccttaagc caggcgatta ctaacggttt tcagcgcagt atgttttcca
     2761 atgaggtggg aatgggatcg acgccaaacg cggcagctgc atcctggcct ccgcatccgg
     2821 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
     2881 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
     2941 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
     3001 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
     3061 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
     3121 ccttcgcaac gatcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
     3181 atatataatg gcctgcatgg ctattaccaa tttgaccgcc attttactgc tctcgcctgt
     3241 ggttcatacc attgccagtg attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt
     3301 tgatccgttg cgttatccgg atatcggtcg ccaactttct ccggacgcgt gggatgatgt
     3361 ttcgcaggag taatcacaac tatcgatcaa ctcattctct ttttttgcta aagtcggcat
     3421 aaatttcctg caaggactgg atatgctgat tcttatttca cctgcgaaaa cgcttgatta
     3481 ccaaagcccg ttgaccacca cgcgctatac gctgccggag ctgttagaca attcccagca
     3541 gttgatccat gaagcgcgga aactgacgcc tccgcagatt agcacgctga tgcgcatcag
     3601 cgacaaactg gcgggtatca acgccgctcg ctttcatgac tggcagccag atttcacgcc
     3661 ggcgaatgcc cgccaggcga ttctggcgtt taaaggtgat gtctacaccg gcttgcaggc
     3721 cgagaccttc agcgaagacg atttcgattt tgcccaacag catttgcgaa tgctttccgg
     3781 cttgtatggc gtactgcgcc cgctcgattt aatgcagcct tatcgtctgg aaatggggat
     3841 ccgtcttgag aatgcccgag ggaaagatct gtatcaattc tggggagata tcatcaccaa
     3901 caagctgaac gaggcgctcg cagcacaagg cgataatgtg gtgattaatc tggcgtcaga
     3961 tgagtatttt aaatcggtga agccgaagaa attgaatgcc gagattatca agccggtgtt
     4021 cctcgatgag aagaacggca aatttaagat catcagcttc tacgctaaga aagcacgcgg
     4081 tctgatgagt cgtttcatta ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt
     4141 taatagcgaa ggttacttct ttgatgaaga ttcctccagc aatggcgaac tggtgtttaa
     4201 acgctacgag cagcgttaag tttgtagtca atgccggatg cggcgtgaac gccttatccg
     4261 gcctacaaaa gcatgcagat tcaatatatt gcagaattaa cgtaggcctg ataagacgcg
     4321 ccagcgtcgc atcaggcgct gaatgccgga ttgttacccg gcatttgtca tttagcggtg
     4381 atgtttgcct ggaccatgac cgccgtgatg atcatgagga gctttcttat ggtggcgcgg
     4441 cggtgacggc ggtccgtgtg ggtgccagcg attgcctcgc cattcataat gttgtttcca
     4501 ccagccgtgg tcgcgccagt gacctccatc ccagtaatag ccacgattat cacgatcgcc
     4561 tatctgtaat tttactgacg ggactaacgt aatttccgca gcctgtgctg ccatgggagc
     4621 gaccagaacc agggaaagtg cgagtacgat agattgcatc tttttcacag gttactcctt
     4681 ttatcattgc ccgttatggg tcgatatccg gagattacgc gatgagaacg acggttgtta
     4741 ttctccgcaa tcctaatctc taagttaccg catttattga taatttctcc tttttccctg
     4801 tcgtttttct ccataatttc ttcataaaaa agccgggctg cataaaagca aacccggcct
     4861 gattgagata atgaatagat tttactgatg attcatcatc aatttacgca acgcagaaaa
     4921 atcggcgggc agattatgtg aaagcaaggg taaatcagca cgttctgcca gctcttttgg
     4981 cagatccaac gtttcaccga gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc
     5041 ggtgccgagg aacaagccat attcgcctgg attcaactgg tcacgcagcg cacgatacgc
     5101 tacggcagcg tgcggctccg aggtgtagcc cagttctttt aactcacgca ttgtctgttg
     5161 cgtggtttca tcatccacgg ctgcataacc cagctctttc agttgccaga ttttgcggcg
     5221 gaacaactct tccacacgcg gccagttgtt cggctgactc acgtccatcg cgttggataa
     5281 cgtcgcctga gtcgctttgg gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc
     5341 gttcacgttg gtcgcagcaa taaaacgttt caccggcaga ccgaatgact tcgccagcag
     5401 acccgccgtc aaatcgccga agtttccgct tggcaccgag acaaccagct ggttgcgcgc
     5461 ctcctgcggc agctgcgcaa cagcttcaaa gtagtagcaa atctgcgcca gcaaacggct
     5521 gatgttaatc gagttagccg agtttaaccc cagcgccact ttcagttctt catcatcaaa
     5581 cgcctgcttc accagcgcct gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat
     5641 attgccgccc aacgtacaga acagtttttc ttgcagtgga ctgattttgc ctcgtggata
     5701 gaggataacc actttcacat tcggcaaacc gtagaaagca tgagccactg cagctccggt
     5761 atcaccggag gtcgcggtca gaatggtcac tggcttatca tccgcaatat gggtcagcat
     5821 ttgtgccata aagcgaccgc cgaaatcttt aaatgccagc gttggcccgt ggaacaattc
     5881 cagacaaccg acatcgcttt caacattggc gaccggagcc gggaaggcaa acgccgcgcg
     5941 cacgcgctct tccaggattt cctgtgggat ttcatcacca ataaacgccg agaggatctt
     6001 cgcactgcgg gtgacaaaat ccagcttcag catctcatca atttcagtca ggctgaattc
     6061 cggcaggtcg tgcggaaaaa acagcccctg atttttgccc aacccctggg ttacggcttg
     6121 cgcaaagctg acctgctcgt tgtgatcttt cagattgtag agtttcattt agttttccag
     6181 tactcgtgcg cccgccgtat ccagccggca aatatgaaca aaaccttcct gattttgcag
     6241 gtagttctta cccaaccagt cggcaacgcg ctgggcggta tccggcttgt cacatagagc
     6301 gaacaaggtc gggccggagc cggagatacc gctcactacc gcgccgattt ccgcgaccgc
     6361 ctgccgcgcc tgccggaagc caggcagtaa ccgttcacgg tagggttcag cgataacatc
     6421 tttcatcagc ttcgcggcaa gctcaggctg acgggaatag caggcgtgaa tgaagccagc
     6481 cagatgtcgc ccgtgcgcaa tgcaatcctg gcggcgatac tgcgccggta aaatagcccg
     6541 ggcttctgcc gtcgagactt taattcccgg atacgccagc acccacagcc actcatcaaa
     6601 ccctggcact tgctggctga tgatgtcgtt ttcttcgatc atcaactgca taccaccaag
     6661 aaaacacggt gccacgttgt cgtaatgaat gctgccggag atacgtcctt ccagctcgcc
     6721 catcaaagcc agcaaacgag tgtcattaag cggcttgccg cagtgttcat tcatcgccat
     6781 cagcgccgcg accaccgaac aggcgctgga gcctaagccc gaaccgatcg gcatattctt
     6841 ttccagagtc atcgccactg gaatttgctt gccaagctcc tggcaaaaac gctcccagca
     6901 ctgataaacg atattttccc gtggttctga cggcagctta tcggcaaagc gtccgaggtt
     6961 gttgagactg aatgtctctg ccgcctcaac cgtgactaca tctccgagca atgcaccatc
     7021 aacgggtgcc accgccgccc cgagcacatc aaacccgacg ctcatattgg cactggaagc
     7081 cggggcataa actttaacca tgtcagactc ctaacttcca tgagagggta cgtagcagat
     7141 cggcaaagac accggcagcg gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg
     7201 gcagcggctg ataatagtgg ctataaaagg ccagggcgtt ttcgccattt ttcactttga
     7261 acagcggatc attcccatcc acttcggcaa tcttcacgcg gcagacacca tcttcatcaa
     7321 tattgccaac atagcgcaaa acttttcctt catcacgggc cttcgccacg cgcgcggcaa
     7381 agagatcgtc gagctgtgac agattcgcca taaaagcggc aacatcaccc tcggcgttaa
     7441 actctgcggg cagcacaggt tcaatttcaa tatccgccag ctccagttca cgtcccgttt
     7501 cacgagcgag aatcaatagt ttacgcgcca catccatacc agaaagatca tctcgcgggt
     7561 ccggttcggt ataacccatt tcccgcgcca gcgtggtcgc ctcggagaaa ctcatgcctt
     7621 cgtctaactt gccgaagata taagaaagcg aacctgaaag aatgccggag aacttcacca
     7681 attcatcacc tgcattgagc agattttgca ggttctcaat aaccggtaat ccagccccaa
     7741 cgttggtgtc atagaggaat ttacgccgcg atttttccgc cgcataacgc aactgatgat
     7801 agtaatccat cgacgaggtg ttggcctttt tgttcggcgt aacaacgtgg aaaccttcgc
     7861 gcaggaagtc ggcatattga tccgccactg cctggctgga ggtgcagtca acaatgaccg
     7921 ggttcagcag atgatattct ttcacgaggc gaattaagcg cccgagatta aacggctctt
     7981 tggcttgtgc aagttcttcc tgccagtttt ccagattaag gccatgcaca ctagtgagca
     8041 gagccttcga gttggcaacg ccgcagacac gtaagtcgat atgtttattc ttcagccagc
     8101 tttgctgacg cttcagttgc tccagcagcg caccgccaac gccaccgacg ccaatcacaa
     8161 acacttcgat aacctgatcg gtattgaaca gcatctgatg agtaacgcgc acgccagtgg
     8221 tcgcatcatc gttatttacc acgacagaga ttgagcgttc agaagatccc tgagcaatgg
     8281 cgacaatgtt gatattggcg cgggccagcg cggcaaagaa tttcgccgag atcccacgca
     8341 aggtgcgcat accatcacct accaccgaga taatggccag ccgttccgtc accgccagcg
     8401 gctccagtaa gccttctttc agttccaggt agaactcttc ctgcattgcc cgttcagctc
     8461 gcacacagtc gctttgtgga acgcagaaac tgatactgta ttcggaagat gattgcgtaa
     8521 tcagcaccac ggaaatacgg gcgcgtgaca ttgcagcaaa gacgcgcgcc gccatgccaa
     8581 ccatcccttt catccccggg ccggaaacgc tgaacattgc catgttattc agattggaaa
     8641 tgcccttgac cggtaattcg tcttcatcac ggctggcacc aatgagcgta cctggtgctt
     8701 gaggatttcc ggtattttta atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg
     8761 tgcgggggtg aagaacttta gcgccgaagt aggaaagctc catcgcttcc tggtaggaca
     8821 tcgacttcaa caacctcgca tcgggcacct gacgcgggtc gcaggtatag accccgtcaa
     8881 cgtccgtcca aatctcgcaa caatcggcgc gtaaacaggc agccagcacc gccgcggagt
     8941 agtcggaacc gttacgtcca agcaccacca gttcgccttt ttcattaccg gcggtgaaac
     9001 ctgccatcaa caccatgtga tcagccggaa tgcggcttgc cgcaatacgg cgggtggact
     9061 cagcaatatc gacggtagat tcgaggtaat gccccactgc cagcagtttt tcgaccggat
     9121 cgataacggt aacgttgtga ccacgcgctt ctaacacgcc ggccataatg gcgatcgaca
     9181 ttttctcgcc acggcaaatc agcgcagcgt tgatgctatc cgggcactgc cccaacaaac
     9241 taatgccatg caggacatgt tttatttggg caaattcctg atcgacgaaa gttttcaatt
     9301 gcgccagcgg gaaccccggc tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac
     9361 gttcggcatc gctgatattg ggtaaagcat cctggccgct aatggttttt tcaatcatcg
     9421 ccaccaggtg gttggtgatt ttggcggggg cagagaggac ggtggccacc tgcccctgcc
     9481 tggcattgct ttccagaata tcggcaacac gcagaaaacg ttctgcattt gccactgatg
     9541 taccgccgaa cttcaacact cgcatggttg ttacctcgtt acctttggtc gaaaaaaaag
     9601 cccgcactgt caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt
     9661 acctgtggta atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt
     9721 gtactctgta atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga
     9781 cctaagtcaa taaaatttta atttactcac ggcaggtaac cagttcagaa gctgctatca
     9841 gacactcttt ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct
     9901 gaaaaatact tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa
     9961 acggtgcaac attgccgtgt ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc
    10021 gaccagtttt atgtcatctg ccactgccag agccgtcagc aatgccatga ctcgttcgcg
    10081 taaagcttgc agttgatgtt ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa
    10141 tgttgctaat tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc
    10201 cgccaccatc ggcacaccag taagcacgtc agccaacgct aactcttcgt tagtcagccc
    10261 ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa
    10321 cagcggcacc agttctactg gcgtggcgta gtaatgatat ttcgcccgac tacgcgcagt
    10381 ggtggcgaca gtgaaatcga catcgtgtaa cgattcagcc agtgtcggga aaactttaat
    10441 attatcaata atatcaccag atccatgtgc gacccagcgg gtggctggct ccaggtgtgc
    10501 ctgactatcg acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc
    10561 cccaatattt tctgctctgg cgggtgcgac caggataatc gttatacgca tattgccact
    10621 cttcttgacc aaataaccgc gaacagggtg atcactgtca acttattacg cggtgcgaat
    10681 ttacaaattc ttaacgtaag ccgcagaaaa agccctttac ttagcttaaa aaaggctaaa
    10741 ctattttctg actgcactaa cggtttaatt gttaaaaaat gctacatatc cttctgttta
    10801 cttaggataa ttttataaaa aataaatctc gacaattgga ttcaccacgt ttattagttg
    10861 tatgatgcaa ctagttggat tattaaaata atgtgacgaa agctagcatt tagatacgat
    10921 gatttcatca aactgttaac gtgctacaat tgaacttgat atatgtcaac gaagcgtagt
    10981 tttattgggt gtccggcccc tcttagcctg ttatgttgct gttaaaatgg ttaggatgac
    11041 agccgttttt gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt
    11101 gttgacgttg atggaaagtg catcaagaac gcaattacgt actttagtca tgttacgccg
    11161 atcatgttaa tttgcagcat gcatcaggca ggtcagggac ttttgtactt cctgtttcga
    11221 tttagttggc aatttaggta gcaaacatgc agaccccgca cattcttatc gttgaagacg
    11281 agttggtaac acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg
    11341 aagcgacaga tggcgcggaa atgcatcaga tcctctctga atatgacatc aacctggtga
    11401 tcatggatat caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc
    11461 aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg
    11521 gcctcgaaat cggtgcagat gactacatca ccaaaccgtt caacccgcgt gaactgacga
    11581 ttcgtgcacg caacctgctg tcccgtacca tgaatctggg tactgtcagc gaagaacgtc
    11641 gtagcgttga aagctacaag ttcaatggtt gggaactgga catcaacagc cgttcgttga
    11701 tcggccctga tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc atgcttcact
    11761 tctgtgaaaa cccaggcaaa attcagtctc gtgctgaact gctgaagaaa atgaccggcc
    11821 gtgagctgaa accgcacgac cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt
    11881 tcgaatctac gccggatacg ccggaaatca tcgccaccat tcacggtgaa ggttatcgct
    11941 tctgcggtga tctggaagat taatcggctt taccaccgtc aaaaaaacgg cgctttttag
    12001 cgccgttttt atttttcaac cttatttcca gatacgtaat tcatcgtccg ttgtaacttc
    12061 tttactggct ttcattttcg gcagtgaaaa cgcataccag tctatattac gagtcacaaa
    12121 catcatgccg gccagcgcca ccaccagcac actggttccc aacaacagcg cgctatcggc
    12181 agagttgagc agtccccaca tcactccatc cagcaacaac agcgcgagga taaacaacat
    12241 gctattgcgc cagcctttca ataccgcctg caaataaata ccgttcatta acgccccaat
    12301 cagactggcg attatccatg ccacggtaaa accgatatgt tcagaaagcg ccagcaagag
    12361 caaataaaac atcaccaatg aaagccccac cagcaaatat tgcattgggt gtaaacgttg
    12421 cgcggtgagc gtttcaaaaa caaagaacgc cataaaagtc agtgcaatca gcagaatggc
    12481 gtacttagtc gcccggtccg ttaattggta ttgatcggct ggcgtcgtca ctgcgacgct
    12541 aaacgcgggg aagttttccc agccggtatc attgcctgaa gcaaaacgct caccgagatt
    12601 attagcaaac cagctgcttt gccattgcgc ctgaaaacct gactcgctag cttcccgttt
    12661 ggctggtaga aaatcaccta aaaaactggg atgcggccag ttgctggtta aggtcatttc
    12721 gctattacgc ccggcaggca ccacagaaag atcgccggta ccgcttaaat tcagtgccat
    12781 attcagctgc aggttctgct tacgccagtc cccttcgggt aaagggatat gcacgccctg
    12841 cccgccttgc tctaacccgg taccgggttc aatggtcagt gccgttccgt taacttcagg
    12901 cgctttcacc acaccaatac cacgcgcatc cccgacgcta atcacaataa agggcttgcc
    12961 taagatgata tttggcgcgt tgagctcgct aagacgcgaa acatcgaaat cggcttttag
    13021 cgttaaatca ctgtgccaga cctgaccggt ataaatccct atcttgcgtt cttccacgtt
    13081 ctgattgcca tcaaccatca atgactcagg taacaaaaaa tggataaaac ttcgtttccg
    13141 ctccacggtt ttatcctctt cctgcaccgt ataaagctcg gtcacaggaa tagcgataag
    13201 cggcccaacg agtttttgcg gcccgctggt actttggcga atcgcatctt ccacatcgct
    13261 acggtaatcg gcacgttcga caatcacttg ccgaatcagc attatcggaa tcaacaacag
    13321 caatactgca ccaaacaagg tggtcatttt ccagaacagg ggggatttca acatagtctt
    13381 ctcctttgca atggcagcag gatacgaagg ctatgtggga agaatttgaa gctatgtgaa
    13441 gtgacggtga agtcgtagcg aggccagcac gccaccttcc tgcacgttgc gcagcgtgac
    13501 ttcgccgtta aacaaacggg cgacctcact gacgaacgcc aaccccagac cgctgctttt
    13561 ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc
    13621 aggaatacca ctaccggtat ccagcacctt aagcgtgacg tgttcctgat ccacttcggc
    13681 gcttagcgtt atgcaaccgc tctcgggagt aaaatcgatg gcgttatcca gtaaattccc
    13741 cagcgcctga tccagtaacg ccggttcagc agcaacatta acctcggtgg gcataacatg
    13801 caaagtgatg tttttttctg ccaactgcac ggtgcgcgct tcgctgacgc ggcgaaataa
    13861 tgccgccaca tcaacagcag tcagaacgac ttcctgacga ttctccagtc ttgcctggcg
    13921 tagtaacgtt tccaccagcg cctgcattcg cgcattttgt gtcagaatgt tgtcagtaaa
    13981 acgagccact acttcaggcg gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc
    14041 cgccagtggg ctttttagct catgggttaa cgcataaaca tactgctcaa tatagttttt
    14101 cccttccagc ttcacgcgca tactttccag cgcctgcgcg agtttacgca gctcgctact
    14161 accgagatcg ggaagaggaa cgggcttatt gtcagtgacg gaatcagcat agcgagtgag
    14221 ccgggcaatg gagcggttga tccaccaaac catgcctgcg ccaatcacca gtgcaatccc
    14281 caacaaaatg gcgctggccc ataaaattcg ccgctcgctg cgtttaatga ccggagccat
    14341 cgccgcgttc ggtttgccta cgctcaaaac gccaataagc cgcgagccgc ccataatcgg
    14401 cgcggcaaca tacatcaccg aactttcggg atcggcagga ttttgcaacg tgctgcgcgc
    14461 accatactga ccacgcaacg ttagccagac gtcattccag cgcgaataat cctgtccaac
    14521 ggctttattt gccgaatcga acaatacttt gccatgcgca tcggtcatat agacatgata
    14581 ttcattgcgc actttgttaa tgccaccaat attggcgcga aacgggcgat gttgtagctg
    14641 attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc
    14701 cagctccgca agtaacgttg ccgtgtcgat caacgtcccc tccgttgctc ttcgcacgcc
    14761 cggtttaact tctttgacaa aaatagccag tacgaaccag gccgccaccg ccaccagtaa
    14821 aaaatagccc agcagcaacc gcatgccgat acgcattaca agcccctcag gctatatccc
    14881 atgccgcgat gagtattaat cggtgaaaga tcggggttga tggcgcgcag cttggcacgc
    14941 agcgttttaa tgtggatatc gacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg
    15001 ctatccatta gttgctggcg ggaccagacg cggcctggcg acttgagtaa cgtcttcagc
    15061 aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg
    15121 ggttcattca attcaaaatg accaatacgg atgacgggag acggcgtcga gaacttcttc
    15181 acccgacgca gtaaggtgcg caccctggcg cacacttcgc ggggtgaaaa cggtttagcc
    15241 acatagtcgt cagcgccaat ttccagccca agcaggcgat cgacctcttc acttcgggcc
    15301 gtcaggaaca gtacaggtaa cgccggatgg agcgccagta actggcggca caattcaaag
    15361 ccactgatat caggcaaacc aacgtcgaga atcatgacgt cgggaacctg ctggcgagct
    15421 ttatccagca ccggcaaacc tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac
    15481 atgtagacca gcgtgtcggc tatcccttgc tcatcttcca ctaaccagac cgtttcccgt
    15541 tgcataaatc gcctctgtta ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt
    15601 ttcggcgaac cttctacaac tttgtcggag taagccagat aagcgagcgc gttgcgtttg
    15661 gcgtcataaa agcgcacgac ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat
    15721 actacctcgc cctgagcttt gccgttttta atacgatccg acagttcaat cggcccgact
    15781 tgctgacaag aaatggccgc atcggaggta tcttctgcca ggcccaatcc ccctttaata
    15841 ccaccggttt tcgcccggct cacataacag gtgacatttt tcacatcggg atcgtcaaag
    15901 gcttccacaa caattttgtg atccgggccg atcattttaa atacggtgtc gaccgaacca
    15961 atctcttctg catgagccaa tggccccagc attatcaggc ttaaagacag gatcaaatgc
    16021 ttgtatttca tattgttacc atttttatct attacacgag caattagttc gtcacggtaa
    16081 ataacatatt gattaagaac aaaatctcaa ttcttttatt tccgtcaagc cctaaaacat
    16141 actctactaa gaaaaaaaca ctgaatgcta aaacagcaaa aaatgctatt atccaattac
    16201 ctgatgtcag gtgctcgttg ttgaaaggat gaggatattt tatggatcag gccggcatta
    16261 ttcgcgacct tttaatctgg ctggaaggtc atctggatca gcccctgtcg ctcgacaatg
    16321 tagcggcgaa agcaggttat tccaagtggc acttacagag aatgtttaaa gatgtcactg
    16381 gccatgctat tggcgcgtat attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac
    16441 gcctgactgc gcgtccgatt ctggacatcg cgctgcaata ccgcttcgac tctcaacaga
    16501 catttacccg cgcattcaag aagcagtttg cccagactcc tgcactttac cgccgttctc
    16561 ctgaatggag cgcctttggt attcgcccgc cgctacgtct gggtgaattc acaatgccag
    16621 agcacaaatt tgtcaccctg gaagatacgc cgctgattgg tgttacccag agctactcct
    16681 gttcgctgga gcaaatctct gatttccgcc atgaaatgcg ttatcagttc tggcacgatt
    16741 ttctcggcaa cgcgccgacc attccgccgg tgctctacgg cctgaatgaa acgcgtccga
    16801 gtcaggataa agacgacgaa caagaggtat tctataccac cgcgttagcc caggatcagg
    16861 cagatggcta tgtactgacg gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt
    16921 ttacctatga aggtctggga accggcgtgc aggagtttat cctgacggta tacggaacgt
    16981 gcatgccaat gctcaatctg acgcgccgta aaggtcagga tattgagcga tactacccgg
    17041 cagaagacgc caaagcggga gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta
    17101 tccgccgtta acgctgcagc tcatctaatg caggggcgtc cagatgcgag atgtcccctg
    17161 cggtttcaac gacccagcca gacgccagcc acaggctttc ctgataatcc acgcgcgaaa
    17221 tagaacagtt acgcagacgt aagcggcgct ctacccatgc tggtaatccg agaatcgtac
    17281 tcaccagaca tcccagtgca ataccatggc tcaccagcag cggtcggctt ccctgcggta
    17341 agtcccggca agattccagt gccgcattga cgcgatcgct gagctcctgc attgactcgc
    17401 cttcaggaat acgcccgtca acggtgccat tgaccagctg ccgacgccag ttctcttctt
    17461 cttcggtcag agaatcgata tgtctttttt ccagcacacc catgtttaat tcacgcaggc
    17521 gagaatcaaa gatgatgtca cagccgcagg cctgggcgat gatttccgcc gtacgccggg
    17581 tgcgtcctaa atcgctactg ataatatgcg taatgccaag ctctttggca cgggttgcca
    17641 cctgcatcgc ctgttgctca cctttggcgg tcagcgggct gtcagactgg ccctgaatac
    17701 gtcgctcggc gttccactgc gtttcaccgt ggcggactag gtatacctgt aacatgcttt
    17761 ttttccgtat actgctgtaa cgttaatttt ctgagtaatg ctgattatgc accaagttgt
    17821 ctgtgcgacc accaatcccg ctaaaattca ggccattctg caggcatttc acgagatctt
    17881 cggcgaagga tcctgccata ttgcatccgt tgccgtcgag agcggtgtac cggaacaacc
    17941 ctttggcagt gaggaaacgc gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt
    18001 acttccagag gctgattttt gggtggcgat tgaagctggc attgatggcg acagcacttt
    18061 cagttgggtg gtgattgaaa acgccagcca gcgcggcgag gcgcgttcgg caactttgcc
    18121 gttacctgcg gtgattctgg aaaaagttcg tgaaggcgag gcgcttggtc ccgtgagtcg
    18181 cgttataccg gtattgatga gattggtcgt aaggaagggg cgatcggcgt gtttaccgcc
    18241 gggaaactca ctcgcgccag cgtctatcac caggcggtga tcctggcgct gagtccgttt
    18301 cacaatgccg tgtatcaggc attgtaggac ggataaggcg ttcacgccgc atccgacatt
    18361 cggtgcacga tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa atcaatcgct
    18421 tttcagcaac acctcttcca gccactgacg cagctccacg ggcgcggctt tcaggctgtt
    18481 agatccacgc gtaatcgtcg cgatgcccgc gccgagttca ttttttaact cacgctgact
    18541 catttcgcca cgcaacagct cttcgacaat acgcacgcga gtccccaacg cttcgcgctc
    18601 atctggcgtc agcatcaggt ttaacaacgg taaatggaga tcgttttggt aggcattctt
    18661 aagcaggtcg acaaaacgta accactcctg gtgacgctgt tctgccatcg ctgctgaata
    18721 gggtgattgt tgggccataa tatgtcgcca ttgttagcgg gggaagcaaa atgactcccc
    18781 cggttgtact cgctaaagag tacgatagca tatcataaac gtgcggatca gtaacgacgt
    18841 ccccattccg tggcgctcat caacgtcggt ttatccccca tgaaatagcg gtagtaagca
    18901 tcataagcca gcacgttctt cacataaccg cgcgtctcgg agaacggaat actctcgaca
    18961 aatgccacag cgtcgatacg cccggcgctg ttgccaagcc aggttcgcac ccgtccaggt
    19021 ccggcgttat aagctgctga ggagaaaata cggttattgc caaactgctg ataaacatat
    19081 tgcaggtaac tggtgccaat gttgatgttc gtttccggat ccagcaattg cccagggctg
    19141 ctataaccag gaatagagaa catcttcacc gtatgggtcg ctgtaccagg cataatctgc
    19201 atcaggccgc tggcccctac cggtgatttc actttcggat tccaggcgct ctcctgacgg
    19261 gcaatcgcca tcgcatagct ttgcgggatc tctttcccac tggtgtaacg tttgaaaaga
    19321 tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc aaagcttccc ggcgatcgtt
    19381 gcctgaacgc taagatccca ccattggtta ttgaaagcat accgagccag ttgagcctgc
    19441 tctgtttttg acttgctctt caccagattg gcccactcgc tacgcgcggt attatcgaga
    19501 ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc cctgagtcag ggtgctgtca
    19561 acattctgcg gcgctttatc aatcttcagc tcatactctt caccgatgcg ttgtgctgca
    19621 accatcgggt agaaaccacg ctgttgtatg agttgatgca aaatctcttt cgcttcagct
    19681 tcacgtccgc gttccagcaa taaatccgcc tgccagtaac gccattcatc tttctctttt
    19741 gcttccatcg gcagacgcgc cagccaggta ttcaggccgc gacgatcgcc ggtgccaagc
    19801 gccattcgca cacggcgttc aatcaacgac gtggattgcg agcgcataat ggcgtcatcg
    19861 cgccatttcg cctgctcatc ggtgacatca ttgcccatca aacgccaggc gacgatatcg
    19921 cgcagctcct gaatctgatc ttcattaagc tgctgcgcct gggcaagcga cgggatcatc
    19981 agccgtgcat tttcggcatc ctgccgcgcc acactggcaa acgccaccgc cgccatttga
    20041 cgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca gtaccgtatt agggttgttc
    20101 gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg caggcatctg ccctgccagc
    20161 actgttacca ggcctgtgtt acccgctttc atcgccagac ggatacgctc taaatacgcc
    20221 agcggatctt gtttacctga cgcacgccag acgctaaata acttgtcaca ggcgttaggc
    20281 tggctcttgc cggttagcca cagctctttc gccccttgcc aggcttcttc actctgcccg
    20341 gtgttccatt tcgcatagta gtaattacat tgcgcttcgg tagttccggg cttttccggg
    20401 ctaaaggcta acaagccacg ccagtcttca cgccgcgcca gttcattgac gaaacgagat
    20461 tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa caaagttagt gaccgtcacc
    20521 gccggttgat tcatcaggtc atcggtgatc tggcggtatt ccaggtaggg ataaagcgga
    20581 taatccttca gtccaggcat catttgttcg accacatcca tctgtcgatt atcccaggcc
    20641 tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg agtcggctcg cgccacgctg
    20701 ctgaccgtca gcagacagac accggcagcc aacagccgcc aggtaacttg tttggctttt
    20761 tccacaagcg catcctctaa gtgtaaatac atcaatgcag cgtcatgccg cgtaatagat
    20821 ttatgctaac cagtcattgc cgtttacgcc acgttacgga cactttttta cttttactgc
    20881 gaaggtgatc gaccaactgg gattaggcgg ggaaacaggc tacacttcgc ctttgaaaac
    20941 tcatcatcac gataaacata gaggcgaagt ccaacgtggc tcaattcgtt tataccatgc
    21001 atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt gaaaaacatc tctctgagtt
    21061 tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg cgcgggtaag tccaccctgc
    21121 tgcgcattat ggcgggcatt gataaagaca tcgaaggtga agcgcgtccg cagccagaca
    21181 tcaagattgg ttatctgccg caggaaccgc agctgaaccc ggaacacacc gtgcgtgagt
    21241 ccattgaaga agcggtttca gaagtggtta acgccctgaa acgcctggat gaagtgtatg
    21301 cgctgtacgc cgatccggat gccgattttg acaagctggc cgctgaacaa ggccgtctgg
    21361 aagagatcat tcaggctcac gacggtcata acctgaacgt acagctggag cgtgcggcgg
    21421 atgcgctgcg tctgccagat tgggacgcga aaatcgctaa cctctccggt ggtgagcgtc
    21481 gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga catgctgctg ctcgacgaac
    21541 cgaccaacca cctggatgcc gaatccgtgg cgtggctgga acgcttcctg cacgacttcg
    21601 aaggcaccgt ggtggcgatt actcacgacc gttacttcct cgataacgtt gcaggctgga
    21661 tcctcgaact ggaccgcggt gaaggtattc cgtgggaagg taactactcc tcctggctgg
    21721 agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga agcggcgcgt cgtaagtcga
    21781 tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg ccgccagtcg aaaggtaaag
    21841 cacgtctggc acgctttgaa gagctgaaca gcaccgaata ccagaaacgt aacgaaacca
    21901 acgaactgtt tattccacct ggaccgcgtc tgggcgataa agtgctggaa gtcagcaacc
    21961 tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct gagcttctcg atcccgaaag
    22021 gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa atcgaccctg ttccgtatga
    22081 tctccggtca ggaacagccg gacagcggca ccatcacttt gggtgaaacg gtgaaactgg
    22141 catcggttga tcagttccgt gactcaatgg ataacagcaa aaccgtttgg gaagaagttt
    22201 ccggcgggct ggatattatg aagatcggca acaccgagat gccaagccgc gcctacgttg
    22261 gccgctttaa ctttaaaggg gttgatcagg gtaaacgtgt tggcgaactt tccggtggtg
    22321 agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg cggcaacatg ctgctgctcg
    22381 acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc gctggaaaac gccctgctgg
    22441 agttcccggg ctgtgcgatg gttatctcgc acgaccgttg gttcctcgac cgtatcgcca
    22501 cgcacatcct ggactaccag gatgaaggta aagttgagtt cttcgaaggt aactttactg
    22561 agtacgaaga gtacaaaaaa cgcacgctgg gcgcagacgc gctggagccg aagcgtatca
    22621 agtacaagcg tattgcgaag taatgcgtaa aatgccggat gcggcgcgaa cgccttatcc
    22681 ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt aggcctgata agacgcggta
    22741 agcgtcgcat caggcattga gcaccgaatg ccggatgcgg cgtgaacacc ttttccggtc
    22801 tacaacagcc ttagcatcct caatttcgtg gcgggtcacc cgtgcaggtt ttttcaaacc
    22861 ttgtttaatc tcgacagcct cttccagaga ggttttcaac tcatcaaaaa aactcatttt
    22921 atacctcgcc tttgagcagt ttcgtcacgg ttatccttgc tcccccatca tctcccgcac
    22981 cagctccacg cagcgcagga aacggctgtc gtaatcgtcc tcttcaaccc gcacgaattc
    23041 gatattgttc tcttccagca tctccaccag caagttctgg aactctttgc gatccaccga
    23101 actgccgagg ctgcgtaaac catccgccac ccacggcgtg ttgttctcca gcaggatcac
    23161 cagatcgaaa cggtattcat caatcagcgc ctgcacgaac ggatgctcac gcccttcgta
    23221 ctttttgcag aacgcctggg tagtgacaaa atcggtgtca ataaacgcca ctttattggc
    23281 gtatttcact gcaaaatcaa tgtattgtgc gtggcccaac gcgattttat cgtagtcgga
    23341 atactgcaat gcgatctcat caccgccgag gtgtgaaaag acataatcgc ggccatattc
    23401 ccacgcactg gtggtgttga agatattggc aagtttgttt accagggtgg atttaccgct
    23461 cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac ggcttcactt cggtaggaat
    23521 atattcccag tagcggaacg ggttttcgcg gatctgcgca ccgctgatac tcataaaggt
    23581 acgtttcgga tcgaccagca ccgtctcgat ccccagatgt tccatatact gtggcgcatc
    23641 ggcttcttcc gaggtgtaga tcaggtccgg ctggatcccc ttttcagcca taaacttttt
    23701 gatgccgttg ctccacacat cccagccatg cggatacggc tccatgccct cttcgttgaa
    23761 agcatgaata cggatatttt tctgatattt aaaggtttgc aatagccagc gcagacgatc
    23821 cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc
    23881 gtcaaaaccc ataatgatat gcagctcgtc aacctggcta caggcgcgct ggataaggta
    23941 gatatgtccg gtatgcagcg ggtagaactt accgaatacg acaccaatgg ttttcttctg
    24001 ccggggaaat tcaagcccca aaaaacggtg caatgcctcc agcttttgcg cgctggggct
    24061 tttaattttg gcattcagta actggcttaa ataccctttg gtcataccgc tggcatcagc
    24121 tacctgctgt agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat caaatgacga
    24181 catatctccc tccgtatctc tcattataag tcgtcgaaca cgctaagcgc gtcggagagt
    24241 tttttaacgc caaaaatctg catcccttcc ggcgcttttt tcggtacgtt agccgccgga
    24301 acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaaa tccgctcctg gccactgggc
    24361 accgggcgga tctcccctgc cagcccgact tcaccaaaca ccaccagatc ctgtggcagc
    24421 ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg ctaagtcgcc gctggtttcg
    24481 gttaccttca cgccgccgac cacgttcaca aacacatcct gatcggccat ttgcagacca
    24541 ccgtgacggt gcaagacagc cagcaggatt gccagacggt tttgttccag ccccactgcc
    24601 acgcggcgcg gattcgccat tatcgagtga tcgaccagcg cctgaatctc caccagcaac
    24661 ggacgcgttc cttcccacac caccatcacg gagctgccgg aggtcacttc atctccgcga
    24721 cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc cctgttcggt catcgcgaag
    24781 acgcccagct cattcaccgc gccgaagcgg tttttatggc tgcgtaaggt gcgaaaacgg
    24841 gagtcggcat cgccatccag aagcaccgaa cagtcgatac agtgttccag cactttcggg
    24901 ccagccagcg aaccatcttt ggttacgtgt ccaaccatga caatcgccac accgcgcgtt
    24961 ttggcgaaac gcgtcagata agccgccgtt tcacgcacct gcgccacgct accaggcgat
    25021 gattgtacat ccgccatatg catcacctgg atcgagtcaa ttaccatcag cttcggttgc
    25081 tcttcttcgg caatcaggca gatctgctcg atgctggttt ccgacaacat attgagattg
    25141 tcagtcggca ggccaaggcg atgagcgcgc attgccacct gttgcagcga ctcttcgccg
    25201 gtgacataca gcgttttcat ctgctgggcc agtttgcaca gcgtttgcag caacagcgtg
    25261 gatttccccg caccagggtt accgccaatc agaatggcac ttcctggcac cacgccgcct
    25321 cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg
    25381 atatcggaga gtttctggac ttttgccacc ccggcgctac cggcatagcc gctgagacgc
    25441 tcgttacgcg ccaccgttgg cgacgcagca agacgcacct cggtgatggt gttccaggca
    25501 tgacaggcac tgcactgccc ctgccagcgc ggataatcgg ccccgcattc attacaaaca
    25561 aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta atgctgtgcc gcccgcagga
    25621 tgacgggcag gcaattactt ctgattcagg ctgccagaga ggatgcagaa tacccccatc
    25681 aggtcagcgt gacggatggt gacttccgcc ttttcattca cttttggctt ggcatggtag
    25741 gcaatcccca gccctgctgc tttgatcatc ggcaggtcat tagctccatc gccaatcgcc
    25801 acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc gagtcagagt tttcgctttg
    25861 tactgcgcgt ctacgatgtc gccgatcaca ttgccggtaa atttaccgtc catgatctcc
    25921 agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc gcaggtattc agcaaagaaa
    25981 gtaaagccgc cggaggcaat cgccactttc cagcccagcg tttccagctt gagcaccagc
    26041 tgcgttaagc ctggcatcag cggcagattt tcacgcacct gttgcagaat attggcgtca
    26101 gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg taaaatcgag ttcgccgcgc
    26161 atcgcccgtt cggttacctc cgccaccatc tcgcccgttc cggccagctt ggcaatttca
    26221 tcaatacatt caatctggat ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg
    26281 cgcaggtgcg ggattttccc cagcggggcg acatccagct gcgcttcgtg ggccaggcgt
    26341 gtagcccgtg cggtgagtga acctgccaga cgaatcacct gataatcttc cacgcaccag
    26401 gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt attgggtcag acgctgttta
    26461 tccagcccac gaccatacag cagccagccg ctacgacctg cgtggtaatc cagtggcatc
    26521 acttcatcac cacttaatga aagaggcaga cccggccata aagagacatc ttcaggcagg
    26581 tcgcaccagg taatgttagg cattaaggct cctgtaaaat cgttcgaagc agggaaaata
    26641 acgcatgagg ctaccttgta tccattgctt ctggcaacat taagtctcaa attttcaaag
    26701 ggtggaagat ggctcgcaca aaactgaaat tccggctaca tcgggcagtg attgtcctgt
    26761 tctgtcttgc cttgttagtg gcactgatgc agggggcgtc atggtttagt caaaaccacc
    26821 agcgacagcg taatccacag ctggaagaac tggcccgcac cctggcgcgt caggtgacgc
    26881 tgaacgttgc accgctgatg cgtaccgact caccggatga aaaacgcatt caggcgatcc
    26941 tcgatcagtt aacagatgaa agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg
    27001 atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga tcggctggcg ctcgacggta
    27061 aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc aattgcgggt aaaaacggac
    27121 cgctcggcta tctgcgcctg acactcgaca cccatacgct cgccaccgaa gcccaacagg
    27181 tggataacac cactaacatt ttacgcctga tgttgctgct ctcactggca atcggcgtag
    27241 tgctgacccg cacgctgcta cagggtaaac gcacccgctg gcagcaatcg cccttcctgt
    27301 taaccgccag caaaccggtg ccggaagagg aagaaagcga gaaaaaagag tgacccatta
    27361 ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct catctctgac tcttacgacc
    27421 cgtggtttaa cctggcggtg gaagagtgta tttttcgcca aatgcccgcc acgcagcgcg
    27481 ttctgtttct ctggcgcaat gccgacacgg tagtaattgg tcgcgcgcag aacccgtgga
    27541 aagagtgtaa tacccggcgg atggaagaag ataacgtccg cctggcgcgg cgcagtggcg
    27601 gtggcggcgc ggtgttccac gatctcggca atacctgctt tacctttatg gctggcaagc
    27661 cggagtacga taaaactctc tccacgtcga ttgtgctcaa tgcgctgaac gcgctcggcg
    27721 tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa aaccgtcgaa ggcgaccgca
    27781 aagtctcagg ctcggcctat cgcgaaacca aagatcgcgg cttccaccac ggcaccttgc
    27841 tactcaatgc cgacctcagc cgcctggcaa actatctcaa tccggataaa aagaaactgg
    27901 cggcgaaagg cattacttca gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg
    27961 ggctcaccat gagcaggttt gcgaggccat aaccgaggcc tttttcgccc attatggcga
    28021 gcacgtggaa gcggaaatca tctccccgga caaaacgcca gacttgccaa acttcgccga
    28081 aacctttgcc cgtcagagta gctgagagtg gaacttcggt caggctccgg cattctcgca
    28141 tctgctggat gaacgcttta gctggggcgg cgtggaactg catttcgacg ttgaaaaagg
    28201 ccatatcacc cgcgcccagg tatttaccga cagcctcaac ctcgcgccgc tggaagccct
    28261 cgccgggcga ctgcaaggct gcctgtaccg cgcggatatg ctgcaacaag agtgcgaagc
    28321 gctgttggtt gacttcccgg accaggaaaa agagctacgg gagttgtcga cgtggatagc
    28381 gggggcggta aggtaactcg ctgattaaaa tatccaagga aggaaatatg atcgatctgg
    28441 aaaaccagga acgggagatt atcaatttaa tgttcagcca gcgtatttcc tggctagccg
    28501 ccgtgcgtat caggcataaa ttatcgcttg ctgaagtaag taaaatgtta ggtatctcga
    28561 ttaattctct aaaacagatc gaaaagacgg aacggcttag ctcaaacatc aaaagcaaaa
    28621 tggcagaaat ttatggttgt ccaccagagt tgctgatatg cccttcctgg atgacggctg
    28681 aacataaatg aatagattcg tgaatagata cttgaatgtt ttttattttc tatccatatg
    28741 aaaaataata gtaaatctat aaatcagttg ctattgatga atagatacta aaaagccgga
    28801 gcagtccccc actccggctt aacacgacaa tcgcctttca gcgaaacaca attactcttt
    28861 atcgcccagc agaacggatt ccagtgcgat tttgatcatg tcgttgaagg tggtctgacg
    28921 ctcagcggca gtggtctgct cgtgagtgcg gatgtggtca gacacggtgc agatggtcag
    28981 ggctttcgcg ccaaactctg cagccacgcc gtagatacca gccgcttcca tttccacgcc
    29041 gaggatgccg tatttttcca tcacgtcgaa catttcgccg tccggagagt agaacaggtc
    29101 agcggagaac aggttaccca cgcgagcatc aacgcccagt gctttagctg catctactgc
    29161 gttacgcacc atgtcaaagt cagcgatagc ggcaaagtca tggtctttaa aacggatgcg
    29221 gttaactttg gaatcggtgc aggcccccat accgataacg acgtcgcgca gttttacgtg
    29281 cggcagaact gcgccacagg aaccaacgcg gataattttc ttcacgccga aatcggtgat
    29341 cagttctttg gtgtagatgg agcaggacgg gatacccata ccgtgaccca ttacggaaat
    29401 tttgcggcct ttgtaagtac cagtgaagcc cagcataccg cgaacgttgt tcacttcacg
    29461 ggcatcttca aggaaagttt cagcaatata cttcgcacgc agcgggtcgc ctggcatcaa
    29521 aactacgtca gcgaaatcgc ccatttctgc attaatgtgt ggggtagcca ttgttttatc
    29581 ctttttgtga cataacaaag gcggagtcaa cgctccgccc aaatccatca gaacatggct
    29641 ttgccatatt ccatatcaga agtaccaaaa tattttgcca gagtctggcc gatatccgcg
    29701 aaggtttcac ggtgacccag tgagcccggt tttactttcg ggccatatac cagtaccgga
    29761 atgtgttcac gcgtgtggtc agtaccggtc caggtcggat cgcaaccgtg gtcagcggtg
    29821 aggatcagga tgtcgtcatc gcgcagcaga gacatcagct ccggcagacg gcggtcgaac
    29881 agttccagac ccgcagcata accggcgacg tcgcggcggt ggccccagga agagtcgaag
    29941 tcaacgaagt tggtgaagac gatggtgtta tcacccgctt ctttcatctc tttgatggtg
    30001 gcgtcaaaca gcgcgtccaa gccagtcgct ttcacttttt tggtgatacc gcagttggcg
    30061 tagatgtctg caattttacc gacagaaacc acctggccgt gtttttcatc aaccagtttc
    30121 tgcagcacgg tcggtgctgg cggctcaaca gccaagtcgt gacggttacc ggtacgctgg
    30181 aagttaccag ctttgtcacc gataaacgga cgagcgataa cacgaccgat attgtagccg
    30241 ccgttggtca gctcttcacg ggcgatttcg cacagttcgt agagtttatc cagaccgaaa
    30301 gtttcttcat ggcaggcaat ctggaacacg gagtcagcgg aggtatagaa aatcggcttg
    30361 ccggttttca tgtgctcttc gcccagttga tccagaatga ccgtaccgga agagtggcag
    30421 ttaccgaggt aacccggcag attagcgcgt tcgaccagtt tatccagcag ctcttgcggg
    30481 aagctgtttt cgtgatcgga gaaatatccc cactcaaaca gaaccgggac accggcgatt
    30541 tcccagtgac cagacggggt atctttaccg gatgacattt cgtgcgccca tgcgtacgcg
    30601 ccgataactt cagcgttgcc gtccattccc gccggaatga aaccggtaga accttcgtgt
    30661 gctttcgcca gccccagacg ggtcagattt ggcagattga gcggcccttt acgaccatta
    30721 tcagcttcgc ctttggcaca agcttctgcg atatgaccca gggtgtcagc cccgacgtca
    30781 ccaaagcgtt ctgcatcttc tgtagcgccg atgccgaatg agtccagcac cataataaat
    30841 gcacgtttca tatgttctcc gtactcagtg cttcaaattt aaatgatcag atcagtatac
    30901 cgttattcgc tgatacggcg atagacagtt ggtgtgcttt ccggtgcgtt atcggcaagt
    30961 ttaattgccg ctttcaccgc tttcgccgct tcctgccagc tgttttcgtc tttcgcgagg
    31021 ataaccgcca gcggacgctg accgtctacc tggtcgccca gacgcgccat atcagtaaag
    31081 ccgacgctgt aatcgatggt gtcagatgcc tgacgacgac cgccgcccat tgcaaccact
    31141 gccatcccca gcgcgcgggt atccatttca ctgacaaaac cttcggtatc agcatagact
    31201 gctttcgtca gcatcgctgt cggcagatac ttcgcgtagt tctcaacgaa gtcggtcggg
    31261 cctttttgtg ccgctaccat acgaccaaag acttctgccg ctttaccgtt gtccagcacc
    31321 gcctgcaatt tcgcgcgcgc ttcggcgtca tctttcgcca gtttgccgga gatcagcatc
    31381 tccacgcaca gcgccatcgt gacatcaaac agacgcgggt tacgatactc acccgtcagg
    31441 aactgcaccg cttcacgaac ttcaaccgcg ttacctgcac tggaggccag tacctgattc
    31501 atgtcggtga gcagcgcggt ggtgcgcacg ccagcgccgt tagccacgcc aacaatcgct
    31561 tcggcaaggg cttcagagag ttcgtaggtc ggcataaacg cgccgctacc cactttcacg
    31621 tccatcacca gcgcgtccag accttccgcc agtttcttcg ccaggatcga ggcagtgatc
    31681 agcgggatgg agtccacggt tgcggtaata tcacgggtcg cgtagaaacg tttatcagcc
    31741 ggagccagtg aactggtctg accgataatc gccacgccga cgtctttaat aatttcgcgg
    31801 aaacggttgt catccgggaa aatgtcgaag ccagggatgg attccagttt gtcgagcgta
    31861 ccgccagtat gaccgaggcc gcgaccggag atcatcggaa tatagccgcc gcaggctgcg
    31921 accatcggcc ccaacatcag cgaagtgaca tcgccgacgc cgccggtcga gtgtttatca
    31981 acaatcgggc cattcagatg caggcttttc cagtcgagaa cggttcctga atctcgcatc
    32041 gccatggtca gcgagacacg ctcaggcatt gtcatatcgt ggaagaaaat ggtcatcgcg
    32101 agggcggcaa tctgcccttc ggaaatagtg ttgtcgcgaa taccgttgat aaagaaacga
    32161 atttcttcat cgctcagcgc atgaccatca cgttttttac gaataatttc ttgtgcgaga
    32221 aacaaggtaa cctcccgagg aaaagagtat gaggcggctt ttgccggatg cgtctacgcc
    32281 ttatccggcc tacgggtaga tacgtctcgt aggcctgata agcgcagcgc atcaggcgta
    32341 aagcatctta cttagtagct gctggcgctc ttaccgtcgc cgtggcccag cgctttcagc
    32401 aggcttgcca gcaggctgga agcaccaaag cggtagtgac gcgcatcagc ccagtcagca
    32461 ccgaacagtt catctgcgat ggcgagatat ttctgcgcat cttccgcagt acgcacgccg
    32521 cccgccggtt tgaaaccaac ggttttttct acgcccatat cacggatcac ttccatcatg
    32581 atgcgagcgc tttccggcgt cgcgttcaca gccactttac cggtagaggt tttgatgaag
    32641 tccgcacccg ctttgatgga gatttcagac gctttacgga tcagcgcttc gtctttcagt
    32701 tcgccggttt cgatgatcac tttcagcagt acattcgctg ccgcgcaagc ctctttacag
    32761 gctttcacca ggtcaaaacc aacctgctcg tcacccgcca tcagcgcgcg gtacgggaac
    32821 accacgtcaa cttcatcagc accgtaggcg attgccgcac gggtttctgc cagcgcgatt
    32881 tcgatgtcgt cgttaccgtg tgggaagttg gttaccgtag cgatacggat ttccggggtg
    32941 ccctgctctt tcagagtttt gcgagcaatc gggataaaac gaggatagat acagatagcg
    33001 gcggtattgc cgaccggagt tttggcctga tgacacagag caattacttt ctcgtcggtg
    33061 tcgtcgtcat tcagggtggt caggtccatc aatttcagtg cacgcaggct gcttgctttc
    33121 agatcagtca tttcattctc ctggcttgtc gccaataaaa ttcaccttgc gagtttgtta
    33181 gtattctaac atctactccg caacacactt cgatacacat cacaattaag gaaatctact
    33241 tacaagtttg catcactgta atgcgatctg gttcaaataa ttcactttca aatgaatgcg
    33301 tcagtggtgg caaacgcatc aggatcaaag tgaacatcac gaaacttctt acaatggcgc
    33361 atcaccttcg gcataaaagg aaaagaagat gcccacttct catgaaaatg cactgcaaca
    33421 acgttgccag caaattgtca ccagcccagt gcttagcccg gagcagaagc gccattttct
    33481 ggcactggaa gcagaaaaca atctgcctta cccacagctt cctgccgaag cccgccgcgc
    33541 gctggatgaa ggtgtaatct gcgatatgtt tgaaggtcat gcgccgtaca aaccgcgcta
    33601 tgtcttaccc gattacgctc gttttctggc gaacggttcc gaatggctgg agctggaagg
    33661 cgcgaaagat cttgatgacg cactctctct gctgaccatt ctttaccacc acgtaccgtc
    33721 ggtcacatcg atgccggtct acctggggca actggatgcg ttgttgcaac cgtatgttag
    33781 aattctaaca caaaacgaga tcgatgttcg aataaaacgt ttctggcgtt acctcgacag
    33841 aaccctgcca gatgccttta tgcacgccaa tatcggcccg tctgattcgc ccattacccg
    33901 cgcgatctta cgtgcagacg cgcaggtttc accgaacctg acctttatct acgatcctga
    33961 aatcacccct gatgacctgc tgctggaagt ggcgaagaac atctgtgaat gtagcaaacc
    34021 gcacatcgcc aacggtccgg tgcatgataa aattttcaca aaagggggct acgggattgt
    34081 gagctgttac aactcactgc cgctggcggg tggtggcagc acgctggtac gccttaacct
    34141 gaaagccatt gccgagcgca gcgaatcgct ggatgacttc tttacgcgca ctctaccgca
    34201 ctactgccag cagcagatcg ccatcatcga tgcgcggtgt gaattcctct atcaacaatc
    34261 acacttcttt gagaatagct tcctggtgaa agaagggctg attaaccctg aacgttttgt
    34321 gccaatgttt ggcatgtatg ggctggcgga agcggttaac ttgctgtgtg agaaagaagg
    34381 gattgccgcg cgctacggta aagaagccgc cgcaaatgaa gtgggttatc gcatcagcgc
    34441 gcaactggcg gagtttgtcg ccaatacccc cgtgaaatat ggctggcaaa aacgcgccat
    34501 gttacacgca cagtcgggga tcagttccga tatcggcacc acgccgggcg cgcgtttgcc
    34561 gtatggcgat gagccagatc cgatcaccca tctgcaaact gtcgccccgc atcatgctta
    34621 ttattattcc ggcatcagcg acattctgac gctcgacgaa accatcaaac gtaacccgca
    34681 ggcactggta cagctttgcc tcggtgcctt taaagccgga atgcgtgaat ttaccgccaa
    34741 tgtcagcggt aacgatctgg ttcgcgttac cggttatatg gtgcgtttgt cggatttaga
    34801 aaaatatcgc gccgaaggtt cacgcaccaa caccacctgg ctgggcgaag aagccgcacg
    34861 caacactcgt attctggaac gccagccgcg cgtgataagc catgaacagc agatgcgctt
    34921 tagtcagtaa gattatcccc ttctcctgcg ttgacgggcc aggcagtcgt ctggctctgt
    34981 ttttgcaggg ctgcaatctg cgctgcaaaa actgtcacaa tccgtggacg atgggacgtt
    35041 gcaatgactg tggggagtgc gtgccacagt gtccgcatca ggcgttgcag attgttgacg
    35101 gcaaagtggt gtggaacgct gtggtctgcg agcaatgtga tacctgcctg aagatgtgtc
    35161 cgcaacatgc cacaccaatg gcgcaatcca tgagcgtgga cgaagtgctt agccatgtgc
    35221 gcaaagcggt gctgtttatt gaagggataa cggtaagcgg cggcgaagcc acgacccaac
    35281 tgccgtttgt agtggcgctg tttactgcta tcaaaaacga tccgcaactg cgccatctca
    35341 cctgtctggt ggacagtaac ggtatgttga gcgaaaccgg ctgggaaaaa ttactcccgg
    35401 tgtgtgacgg cgcaatgctc gatctcaaag catggggtag cgaatgtcat caacaattaa
    35461 ccggacgcga taatcaacag attaagcgca gcatctattt gctggcagag cgcggcaagc
    35521 tggcggaact gcgtttgctg atgatcccag gccaggtgga ttatttgcaa cacatcgaag
    35581 aactggcggc gtttatcaag ggacttggtg atgttccggt tcgcctgaac gcgtttcatg
    35641 cccacggcgt gtatggcgag gcgcaaagct gggcgagcgc cacgccggaa gacgttgagc
    35701 cgttggctga tgcattaaag gtgcgcgggg tgagccggtt gatatttccg gcgctctatt
    35761 tgtgacatgc cggatacggc ctacggttcg gcacagactt gtaggcctga taagacgcgt
    35821 caagcgtcgc atcaggcatt gtgcgccaac tgccggatgc ggcgtgaacg ccttatccgg
    35881 cctacggttc ggcacaaacc tgtaggcatg ataagacgcg ttaagcgtcg catcaggcat
    35941 tatgcgccga ctgccggatg ctgcctacga agggcaaact acccacgaac gttaaacacc
    36001 gcatacgtgt tattaagcaa cacttcggca atctcatccg cgggttccgg gcgtaactcg
    36061 caaagcacgg caaacacacg ggcagcctgt tccgggcggt taggctgccc ctgaaaaccg
    36121 ttgagcggca tatccggcgc atcggtttcc agcaacaacg atgccagcgg taattttgcg
    36181 atgacatcgc gggttttact ggcgcgtgga taggtgatag taccgcctac gccgattttg
    36241 tagcccagct gtacaaaccg ttcagcctgt tgctggctgc cagaaaatcc gtggacaaca
    36301 ccagcacgcg gcaaatcgtg gtgtttaaga tgcatcgcca gtttgtcgtg cgtgcgccgt
    36361 gaatgcagga tcaccggcag atcgtagcgt ttcgccagtt tcagttgttc gtcgagtaac
    36421 cactgctgcc tctcaaattg cggatcgtcg ccgaagagat ccagaccgat ctcccccacc
    36481 gccaccacct tcgccggacg cctttccagc gcctgctgta gctgctcaag agacacatcg
    36541 ctatgttttt ccaacatacc aggatgcaag cccaatgcgg catacagcgg ttgataattt
    36601 tccgctaacg cctgcacgcg ggcaaaattt tccgcctctg tcgccggaac aatgatcttg
    36661 cctacgcccg cttgtgccgc gcgttgcagg ctggcctctt catcgccaat aaaaggcggg
    36721 aaatcaaaat ggcagtgagt gtcgataaaa cggcaaatca agccagatcc tcattgttaa
    36781 atgtcgggtc gttcgcctgc ggagcgtcgc tcacttcggc aaccagcgta tcgttggcga
    36841 ctggcgcagg cggaatgaca atcgattccg gcgtcaccac tggcaccaga tggcgggtaa
    36901 gcggcgcttt ttcagttaat agcttgccaa ccgtggcgag gaaataacga ccgcataaac
    36961 gcccaagttt atagtcttca cgcagcgccg gaatccgact gccaagcgcg atactatgta
    37021 atggcttcgg cggataaatt tcggatatcc gcagcttgcc cggtggtttc tcaataaatt
    37081 gctgaatgtc acgatagctg gtttcatgat gctgcaccag attgaccagc ggctgcaggc
    37141 tactgtcacc cagccagcgt tccatgcgtt tgaaccactg cggcgtgtag tacatttgtg
    37201 acggcacagt gcggatgacg accaacgttt tagcgccctg ccttgccgcc tctttaaccg
    37261 gaatcgcatc actgatcccg ccatccaggt agttaatgcc ttccagcgac actccgctac
    37321 gataaaagcc aggtatcgca ctggaggcgc gaatcacatc cagccagttt tgtttggttg
    37381 gtaaaaagta attcggctcg tagtcatcct gacgacaggc gcacatataa aacgatttgc
    37441 cgctgtcaaa caaccgcgcg gcggtatcca tttgcaacgg catctggctt gcggtggcct
    37501 ccaccagcca gtcgagatcg ataagatttc ctccacggac aaagcgcaat ggatcgaaaa
    37561 attcgcgttt tgtggtatag cgcatgatga ctttgcgcgc gtaaccgggc tgattgcaga
    37621 taaacgccga gaggttctgc gccccggcag atgtgccgag ataaagatcg aaaggattaa
    37681 actgcgcgcg cataaactca tccagcacgc cagccgtgaa aattccacgc tgtccgccgc
    37741 cttcgcacac cagagctatt cgtccaggct ggaacggcct tagcgacaac ggcgcaatat
    37801 taccaagcgt tacaggtatt cgctgcccca cttctgcctt cctgtttttg ttgtgaaaga
    37861 aagacaaagt aacgcaattt attattcgct aaaaccgcaa aagccagtcc gctggactgg
    37921 ctttggataa aattttctta atggtctgaa atctagggtc gttttcggcc catgaacaaa
    37981 ctcactagga acagaataat cccgacgaca aagacaattt tagctgcgcc tgcagcggta
    38041 ccggccagac caccaaaccc aagtgcggcg gcgattaacg cgataaccag aaatatgatg
    38101 ccccaacgaa acataagcgt ctcctttacc atagttaatg tcaccgcttg atatgagcgt
    38161 gaaaatcact caatgcgata tttttagtgt ggtgcacatc gcgcctcccg acaaagttcg
    38221 ggaggacgaa ttacttagtt ttcagatcat ttttaacgct tttcacacca tctaccgctt
    38281 tggcgatact ttcagcacgg tcactttgtg cctgagaatc tacggtaccg gagagctgaa
    38341 ccacgccatc ggtggtttca actttcactt tacgtgaagg gacgatatcg tccgccagca
    38401 gtttggcttt gatttcactg gtggtggcgg tgtcacccgc gtagcccttc accgagcctt
    38461 ctttagcgtc gcgaacgtgc agtttgtcgc tgacagaggt cacgccttca acgcctttcg
    38521 ccactttcac tgcctcttcg gcctgggcct gactttcaac gaaaccgctc agggtcacga
    38581 ctttttgatc ggttttgaca gagatatcgg tgctcttgat gttgtcatga tccaccagcg
    38641 ccgccttcac tttcgcggtg atggcgctgt catccatgaa attaccgact ttattcatag
    38701 agctatcgac tttttgccct gcgctttcat tggtagtctg cgcgttgttt tccgcgtagg
    38761 cagagccggt cgcgacggca gaggtcaaca ttacagccag cagagttttc gaaatcttca
    38821 gtcttgtcat agtcatcgat ttattcctgt atgtttgctc gtaatttgag cctagcaaca
    38881 cgaggttgca ttgctgaata gggagagact tcaccctcta cagaagtcaa tggtcgccat
    38941 cacaaaagcg atgggtgatg aataacgacc attacagcct ctgaatcagt tattaatatc
    39001 ggtagaatga caatcgacgg ctttagatac tgatatctac gcattgaacg gtatttaacg
    39061 ccatcagaaa tgtcatcact ttgttaaata tagatcacaa ttttgaaacc gctcgggata
    39121 tcagcgaaaa cataagcaaa agtgaatgtt ttaagaacat tccgtaagcg gctaataagg
    39181 aagggaaatt gacagggcgc agcggctacc gcgccctgga ggcaagaatt aatgctcgcg
    39241 ggtctggtgg aactgaacgt ccggataacg ttcctgtgcc aggcgcaggt tgaccatgct
    39301 ggtagcgatg taagcgaggt tatcgccgcc atcaagcgcc agttggcttt cgttcttacg
    39361 cttgaactct tcgaatttct tcgcgtctgc acattctacc cagcgggcag tggcaacgtt
    39421 gactgactca tacactgctt caacgttgta ttcgctcttc aggcgcgcta ccaccacatc
    39481 aaactgcagc acaccaaccg caccaacgat cagatcgttg ttggagattg gacggaacac
    39541 ctgcaccgcg ccctcttcgg aaagctgtac cagccctttg agcagctgtt tttgcttcag
    39601 cggatctttc aggcggatac gacggaacag ttctggtgcg aagttcggaa taccggtgaa
    39661 cttcatcatc tcaccctggg taaaggtgtc gccgatctga atggtgccgt ggttgtgcag
    39721 gccgaggata tcgcccggat acgcttcttc aacgtgcgaa cggtcacccg ccataaaggt
    39781 cagcgcgtcg gagatcacca catctttcgc agtgcgcacc tggcgcagtt tcatgccttt
    39841 ttcatattta ccggacacca cacgcataaa cgccacgcgg tcgcggtgtt tcggatccat
    39901 gttggcctga attttaaata cgaagccggt aaatttatct tcgctcgctt ctacggtacg
    39961 ggtatcggtc tgacgcggca tcggcgcagg cgcccactcc accaggccat tcagcatatg
    40021 atcaacgccg aagttaccca gcgcagtacc gaagaatacc ggagtgattt cgcccgcaag
    40081 gaacagctct ttgtcgaact cgttagacgc gcctttaacc agttccagtt cgtcacgcag
    40141 ctgctgtgcc agatcttcac caaccgcagc atcgagatcc gggttattca gccctttaac
    40201 aatgcggact tcctgaatgg tgtgcccttt accgctctga tagagatagg tttcatcttt
    40261 ataaaggtgg taaacgcctt taaacagctt gccgcagcca attggccaag tgatcggtgc
    40321 gcagccaatt ttcagctcgt tctcaacttc atcgagcagt tccatcgggt cgcggatatc
    40381 acggtcaagt ttgttcataa aggtgaggat cggcgtgtcg cgcagacggg taacttccat
    40441 cagcttacgg gtacgatctt caacaccttt tgcggcgtcg ataaccatca ggcagcagtc
    40501 caccgccgtc agggtacgat aggtatcttc cgagaagtct tcgtgccccg gggtgtcgag
    40561 caggttaacc aggcaatcgt gatacggaaa ctgcatcaca gacgtagtaa tggagatccc
    40621 acgctgcttt tccatctcca tccagtccga cttagcgtgc tggttggaac cacggccttt
    40681 tactgtaccg gcggtctgaa tggcctgtcc gaacagcagc accttctcgg tgatggtagt
    40741 tttaccggcg tccgggtgag aaataatggc aaaagtgcgg cgcttcgcca cctctttcaa
    40801 ataaggagac aacgtcataa ttctttcttc ttgagtaagc ggcatcgcgc cgcgcttgtt
    40861 gaaaacgaaa aattgcggct attttaccca tcaacggggg ggaggcaatc agtgtttaca
    40921 caggagctgc tccagttcgt gcaacgaaga aacggtccag gtgggcgcga tgccttctgg
    40981 ttgctcgcga ttgtgcgcat tcagccagca ggtcgcaagc ccggcgttga tgccaccgag
    41041 aatatcggac tcggcagtgt cgccaaccat cagcacgcgt gaacggtcag gattgcccgc
    41101 ctgttccagc gcataatcga aaattttctt attcggtttg gcaacgccaa cttcttcgga
    41161 aatcaccagc aaatcgaagt aatcacgcag gcccgtccgt tccagacgca cttgctgcaa
    41221 ggcgctaaaa ccgttggtga tgatgccgat tttagcgttg ctacgaatgg cgttaagcag
    41281 agaaaccgcg cccggcaacg gcgtacagat ttccgccatc gcattaataa aggcatcgtt
    41341 aagcaagcct gcttcaactt tcagccgttc ggcccagctc tcgaaacgcc cgtgctgaag
    41401 ctgtaatgaa gtgatcgcgc cgttttgata atccacccac agtggcttgt taacggcctg
    41461 atagtcctga aaatcttcag cggtaaaggt gacgctgtaa tcaagaaaca tccgctgcag
    41521 gccggtgaat gagtcaaagg taaacagcgt ttcatcggca tcaaagaaaa tccagtccca
    41581 cttcattatt ccaccttgtc ttacatactg attggcaacg ccatgatgat ggcgtcttcg
    41641 cgaccgtccg tggtggggta gtaattgcgg cgaatcgtcg cctcgttaaa gcctaaactt
    41701 tcgtacaggg caatggcggc agcatttgag gcacggactt ccagccatag tgtcgccacg
    41761 ccgcgttttt ccagttcgtc gatcagatgt tccagcagcg ccctacccag cccctgacgc
    41821 tgatagtcgg gatcgaccgc aatattgaac aatgtagctt catccagcac cacttgcgta
    41881 atcgcaaacg ccgccatttt gccgttttgc gttaactgaa agttgagata acgctcgccc
    41941 tggttgctgg caaaggtttt ttcactccac ggaaaggagt gggcgcgttg ttcaatgtgg
    42001 taagccgctg gtaaatcagt cgtttcgagg gaagaaatcg tgttcatatg tgcaaatttg
    42061 ttgccataac gcggcgcgtg ccgttgggtt tgcccgtaat tcggtgagcg ccggtgatgc
    42121 cacctgagcg ccttccaatg atagcggttc gtcagtaccc aaccgccagc tgttgcagcg
    42181 actgccttgc ggcagcatcg cgattttttc tggcgtcagt tgcagcactt gatcggggct
    42241 gacggttaat gcgcgcagaa catcgctcac taaaggatca gtcagggcgg gaagatcgtt
    42301 tgccaccatc accagacgga cgtgtgccgg gctcgcaatg gcaatctccc cctgcaacgc
    42361 gccagggcga cgcagcgacc actgggtaat gcccagttgc tgtaactgcc agtctcgtcg
    42421 ggatgtcata gcgaatcgct cctgttgtca ggggcgcaaa tatagcaaat tcgtcgatac
    42481 cgcgccaaca tatggctata atcgccgcca gtatcaattg aggagcattc catgtctgca
    42541 tttaccccgg caagtgaagt cttgctgcgt cacagtgatg atttcgaaca aagccgtatt
    42601 ctgtttgccg gagacttaca ggatgacctg cccgcgcgtt tagataccgc ggccagccgt
    42661 gctcataccc agcaattcca ccattggcag gtgttaagct gccagatggg ggataacgcc
    42721 cgttttagtc tggtcgccac ggcgaatgat gtcgcagatt gcgatacgct gatttactac
    42781 tggccgaaga acaaaccgga agcccagttc cagttgatga atttactttc tctgctgcca
    42841 gtggggacag atatttttgt cgttggcgag aaccgcagcg gcgtgcgcag cgctgagcag
    42901 atgctggcag attatgcgcc gttgaataaa gtcgacagcg ctcgtcgctg tggcctctat
    42961 tttggtcgtc tggaaaaaca gccagtattt gatgcggata aattctgggg cgaatacagc
    43021 gtcgatggcc tgacggtcaa aacgctgcct ggcgtgttta gccgcgacgg tctggatgtc
    43081 ggtagccagt tgctgctctc gacgttaact ccgcacacga aaggtaaagt gctggatgtc
    43141 ggctgtggcg cgggtgtact ttcagttgcc tttgcgcgcc attcgccgaa aattcgtctc
    43201 accttgtgcg atgtctctgc cccggcggta gaagccagcc gcgcaacact tgcagccaac
    43261 ggtgttgaag gtgaagtctt tgccagcaac gtcttttccg aggtgaaagg ttgttttgat
    43321 atgatcatct ccaacccgcc gttccacgat gggatgcaaa ccagcctgga tgcggcgcaa
    43381 acgctgattc gtggcgcggt gcgtcatctt aatagcggcg gcgagctgcg aattgtagcg
    43441 aacgccttcc tgccttaccc ggacgtgctg gatgagacat ttggcttcca tgaagttatc
    43501 gcgcaaaccg ggcgcttcaa ggtatatcgc gccattatga cccgccaggc gaagaaaggt
    43561 tgagtatctg atatctgtcg ccgcataccg tgtcggatgc ggcgtgaacg ccttatccga
    43621 cctacaaaac ctgcacgtta gcccttcgta ggccagataa gacgcgccag cgtcgcatct
    43681 ggcatctgcc cccgcatgcc taccaaacct gcacgctagt ttcctgatgg acatttttcc
    43741 agcaattaca catcaggcga taattaacta ttgacgaaaa gctgaaaacc actagaatgc
    43801 gcctccgtgg tagcaattct ttttaagaat tgatgg
//
LOCUS       DAJVRL010000026        39560 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVRL010000026 DAJVRL010000000
VERSION     DAJVRL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.026"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..155
                     /locus_tag="OQN02_001378"
     CDS             <1..155
                     /locus_tag="OQN02_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1642234.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OQN02_001379"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OQN02_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1642235.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3035
                     /gene="uidA"
                     /locus_tag="OQN02_001380"
     CDS             1362..3035
                     /gene="uidA"
                     /locus_tag="OQN02_001380"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS1642236.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OQN02_001381"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OQN02_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OQN02_001382"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OQN02_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1642237.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OQN02_001383"
     CDS             complement(5626..7134)
                     /locus_tag="OQN02_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1642238.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OQN02_001384"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OQN02_001384"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1642239.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OQN02_001385"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OQN02_001385"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1642240.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OQN02_001386"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OQN02_001386"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1642241.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OQN02_001387"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OQN02_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1642242.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OQN02_001388"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OQN02_001388"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1642243.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OQN02_001389"
     CDS             complement(14283..15602)
                     /locus_tag="OQN02_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCS1642244.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OQN02_001390"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OQN02_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1642245.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OQN02_001391"
     CDS             16699..17034
                     /locus_tag="OQN02_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1642246.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OQN02_001392"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OQN02_001392"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1642247.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OQN02_001393"
     CDS             complement(17790..19172)
                     /locus_tag="OQN02_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1642248.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OQN02_001394"
     CDS             complement(19358..20302)
                     /locus_tag="OQN02_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1642249.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OQN02_001395"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OQN02_001395"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1642250.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OQN02_001396"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OQN02_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1642251.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OQN02_001397"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OQN02_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS1642252.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OQN02_001398"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OQN02_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1642253.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OQN02_001399"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OQN02_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1642254.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OQN02_001400"
     CDS             complement(25947..26768)
                     /locus_tag="OQN02_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1642255.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OQN02_001401"
     CDS             complement(26868..26951)
                     /locus_tag="OQN02_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642256.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OQN02_001402"
     CDS             complement(27044..27379)
                     /locus_tag="OQN02_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS1642257.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPTQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27947..28204)
                     /locus_tag="OQN02_001403"
     CDS             complement(27947..28204)
                     /locus_tag="OQN02_001403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642258.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28220..29029)
                     /locus_tag="OQN02_001404"
     CDS             complement(28220..29029)
                     /locus_tag="OQN02_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1642259.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29136..30038
                     /locus_tag="OQN02_001405"
     CDS             29136..30038
                     /locus_tag="OQN02_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1642260.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="OQN02_001406"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="OQN02_001406"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1642261.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="OQN02_001407"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="OQN02_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1642262.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="OQN02_001408"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="OQN02_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1642263.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="OQN02_001409"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="OQN02_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1642264.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /locus_tag="OQN02_001410"
     CDS             complement(34274..35128)
                     /locus_tag="OQN02_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1642265.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="dmsB"
                     /locus_tag="OQN02_001411"
     CDS             complement(35130..35747)
                     /gene="dmsB"
                     /locus_tag="OQN02_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1642266.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..37796)
                     /locus_tag="OQN02_001412"
                     /pseudo
     CDS             complement(35758..37796)
                     /locus_tag="OQN02_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37857..38966)
                     /locus_tag="OQN02_001413"
     CDS             complement(37857..38966)
                     /locus_tag="OQN02_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1642267.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38949..>39560
                     /locus_tag="OQN02_001414"
     CDS             38949..>39560
                     /locus_tag="OQN02_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1642268.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIG"
BASE COUNT        10066 a         9590 c        10129 g         9775 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaat actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgtaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtgg
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgccag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttacaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccacgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgtggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggtaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctag cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgactagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    36121 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    36181 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    36241 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    36301 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    36361 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    36421 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    36481 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    36541 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    36601 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    36661 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    36721 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    36781 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtga tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct gccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcta ctgtcagacg gatggacgga cttgaagtag ttgactcaac gctacttaag
    38941 gtcctgatat gactgacatt aaccatgacg atactgcata tccatccagt tctttctctg
    39001 ggtctcccta tctgtatcag caagtctaca cctcaagtcc tgacacaagg ctgacacaaa
    39061 tcaaggacct tgctacgcca cagctagtcc ggagaaccaa cgggcgttac actgtccgct
    39121 tcagacttaa aggagctacc agcccgtttc tatcagtctc aacccgcagc acagaccgga
    39181 ggaccgctac aatgcgtcag agagagctcg cagccacagc taaggcattc ctactggaca
    39241 atcctgaagt cacactacag gagcttacag cgcacatacg gtccatggct gagcagttcc
    39301 tgactgatgc cagtgatgac tactggaatg gcgtagaggt ggcgacactg gttgacgaga
    39361 agtccaacct cagggaactt gcagcgacaa gagcgttgag tgtggaccag cagaggggca
    39421 ttaagttagc gctggaagtc ctgaccgcag cacagcagag agtcgacact ggtgatacct
    39481 ctggtctgat taagctgatt gatggtggcg taagccagct tactgatgat aataaccata
    39541 ctgattactc aactataggc
//
LOCUS       DAJVRL010000027        38775 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVRL010000027 DAJVRL010000000
VERSION     DAJVRL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.027"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OQN02_001415"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OQN02_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS1642269.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="OQN02_001416"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="OQN02_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1642270.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="OQN02_001417"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="OQN02_001417"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1642271.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="OQN02_001418"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="OQN02_001418"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1642272.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="OQN02_001419"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="OQN02_001419"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1642273.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="OQN02_001420"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="OQN02_001420"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1642274.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="OQN02_001421"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="OQN02_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1642275.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="OQN02_001422"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="OQN02_001422"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1642276.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="OQN02_001423"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="OQN02_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1642277.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="OQN02_001424"
     CDS             7138..7749
                     /locus_tag="OQN02_001424"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1642278.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OQN02_001425"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OQN02_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1642279.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OQN02_001426"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OQN02_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OQN02_001427"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OQN02_001427"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1642280.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="OQN02_001428"
     CDS             complement(11943..12302)
                     /locus_tag="OQN02_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642281.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="OQN02_001429"
     CDS             complement(12299..12805)
                     /locus_tag="OQN02_001429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642282.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="OQN02_001430"
     CDS             complement(12951..13514)
                     /locus_tag="OQN02_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642283.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="OQN02_001431"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="OQN02_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="OQN02_001432"
     CDS             complement(14722..15096)
                     /locus_tag="OQN02_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCS1642284.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OQN02_001433"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OQN02_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCS1642285.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OQN02_001434"
     CDS             complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OQN02_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCS1642286.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17166..18056)
                     /locus_tag="OQN02_001435"
     CDS             complement(17166..18056)
                     /locus_tag="OQN02_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCS1642287.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OQN02_001436"
     CDS             complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OQN02_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCS1642288.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OQN02_001437"
     CDS             complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OQN02_001437"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCS1642289.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OQN02_001438"
     CDS             complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OQN02_001438"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1642290.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OQN02_001439"
     CDS             complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OQN02_001439"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCS1642291.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OQN02_001440"
     CDS             complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OQN02_001440"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1642292.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OQN02_001441"
     CDS             complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OQN02_001441"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCS1642293.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OQN02_001442"
     CDS             complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OQN02_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCS1642294.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OQN02_001443"
     CDS             complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OQN02_001443"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCS1642295.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OQN02_001444"
     CDS             complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OQN02_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCS1642296.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OQN02_001445"
     CDS             complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OQN02_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCS1642297.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OQN02_001446"
     CDS             complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OQN02_001446"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCS1642298.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OQN02_001447"
                     /gene_synonym="manB"
     CDS             complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OQN02_001447"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1642299.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRPSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OQN02_001448"
     CDS             complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OQN02_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1642300.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OQN02_001449"
     CDS             complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OQN02_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1642301.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OQN02_001450"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OQN02_001450"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1642302.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="OQN02_001451"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="OQN02_001451"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="OQN02_001452"
     CDS             complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="OQN02_001452"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1642303.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQTETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="OQN02_001453"
     CDS             complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="OQN02_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1642304.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38654..>38775)
                     /locus_tag="OQN02_001454"
     CDS             complement(38654..>38775)
                     /locus_tag="OQN02_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1642305.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10356 a         9707 c         8725 g         9987 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgatgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaacccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt ctctgcccct
    17821 ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc attcctacca
    17881 agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt gaaattagtc
    17941 ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat cagtgcattc
    18001 agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact attcattatc
    18061 caagtgactc agtaattggt taattttagt tttcatatac agaacacatt ctagtataaa
    18121 gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt gcggcaccca
    18181 gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc aattcagcac
    18241 aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag aatggtatca
    18301 ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta aaggaattaa
    18361 ttttataatt ctcaccaaac catattttca atacaagccc tgaatataac aagcctatga
    18421 ttatcaccgg caaacagatt agaaacatta aaagcaatga tttatttaca ttacgtttaa
    18481 attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg attgctgcag
    18541 gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca actttatctg
    18601 cccccataat atttgacact ataaatcgat caaaatacac catgaccgga cttatgatat
    18661 tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta taacaaaaat
    18721 gaacccccga aattttaata tcattcctga cataataggc agagactaaa atcgaaataa
    18781 ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa aaaacaaata
    18841 tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt acaatgccaa
    18901 atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat aatggaatgc
    18961 aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca gaaattttta
    19021 gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag catgaaaata
    19081 gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga ttatcacgat
    19141 gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt ccagcatacc
    19201 caacaagtgc aattgcaatt gtataaatac cgaaaccttc aggccccaaa cctcgagcga
    19261 gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa aggttccaga
    19321 cactattttt tattatgctc attattacat ttctttatca tataaaataa tcagcatcta
    19381 caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc ggccattcta
    19441 tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa tagaaattat
    19501 ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga gcgaatcctt
    19561 ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc cacttgccaa
    19621 atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg acaacacagc
    19681 gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt aacacaccct
    19741 tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca gcctcttcga
    19801 aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat acttttggtt
    19861 caaaaattag cacatcagga atttcagttt taattacgtt cattttatta ataaccttta
    19921 atcattttca gcagatactg accataagca tttttcttca gcggctcggc taataccttc
    19981 acttgctcag catcaataaa gcctttacgg taagcaattt cttccgggca ggaaactttc
    20041 agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag actttgatgt
    20101 gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa acgtccttgg
    20161 tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga tggtttaagg
    20221 tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac cgcatagtta
    20281 ctttttggtt ccagcggttt ttcttccaga ctaattgccg taccgttatc atcaaattcc
    20341 accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc gccactctct
    20401 ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat attatcacca
    20461 agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa tgcttgcgcc
    20521 agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg gcttccatca
    20581 ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag aatatctcga
    20641 atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc ataaatcggt
    20701 agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga accaccagcc
    20761 agaataatac ctttacgcgt tttcatttca ccattccttt taattcatct cgctctagca
    20821 tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt cgttgagcat
    20881 acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc
    20941 ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg ttgtcggcac
    21001 ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt caaataccag
    21061 agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca aaccagcaac
    21121 ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca gtaattcagc
    21181 ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt cttttgccag
    21241 gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc tggtacggaa
    21301 aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct tggtttcacc
    21361 gtagacattt agcggtgctg ttgcatccgt ttccagccat ggcgtgtcac cgttgcctgg
    21421 gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag ctgcttttgc
    21481 aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg attccgcttt
    21541 gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt ttttgacggt
    21601 ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt gaacatcgag
    21661 cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca cctgccctgt
    21721 tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc aatccacgac
    21781 tgataagcac cgcttttcac attctcaacc caatttgtat tagccagata ccattccacc
    21841 gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc gcggctaatt
    21901 ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac ataagtaatt
    21961 tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca aatagtgagc
    22021 actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc acccgcttta
    22081 ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa ccagtcgcga
    22141 atttgattcc ctttaccata aataggtaat gccttacctt ccagagcatt cagaataacc
    22201 aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca attagtcaca
    22261 atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct ggaagcttta
    22321 gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag cggtaacgct
    22381 tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc agtagaaata
    22441 tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta attgcgcgcc
    22501 gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc agggcccgtt
    22561 attgaacggt caacgtggct ctctgccgcc aggtgcatca ccgcgtctgg ctggtgctgt
    22621 gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa agcataacgt
    22681 tcagaatcag aaacatcagc gagtgattcc aggttcccgg cgtatgttaa tttatcgaca
    22741 ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga accaataaac
    22801 cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat agagcatgct
    22861 gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta ccgcccctgg
    22921 cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt agccactcgc
    22981 gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga aacaaggaaa
    23041 atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata ggctttataa
    23101 aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct tcactgccgt
    23161 tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc
    23221 tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata cttcacaaac
    23281 gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat cagcattgca
    23341 tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat acgtccccat
    23401 gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac atagcgacct
    23461 acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca
    23521 atgcggctga ctttaccctc acggtccagc ggctctttag tctggatgac ggagtattca
    23581 gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc gttgaaacgt
    23641 gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc gatcacaacg
    23701 tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag
    23761 tggcctaaac ctaaaggttc gccctgacgc acgttcataa tgatcacgcc cggcggacag
    23821 atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag tgattctaac
    23881 tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt taccaggagg
    23941 atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg
    24001 acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccgaga
    24061 cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg taaaatggtt
    24121 gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa ttgattactg
    24181 aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt cctaagtatg
    24241 gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa tctaaagtta
    24301 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgccct
    24361 ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag
    24421 acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    24481 tcctgcatca cattgatatt tcgcataaaa agatgttgcg gcttgttgtg taactccagc
    24541 gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt cccggcggag
    24601 atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat attattcact
    24661 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    24721 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
    24781 actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt gcgggcattg
    24841 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
    24901 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttgttatca
    24961 taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg
    25021 atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc cacgttccat
    25081 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc accgataatc
    25141 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    25201 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa tataaatctg cattaccggg
    25261 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aacaacgcac atccagggta
    25321 atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg taagacaaat
    25381 cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc gggagggatg
    25441 aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac cgtttgccct
    25501 tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc gttaatgtta
    25561 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    25621 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc tcctctataa
    25681 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
    25741 tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtatccagtt ggctaaacgc
    25801 cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc agcagccagc cggatttatc
    25861 ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa taccgaccgc
    25921 catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc ccgttaccga
    25981 tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc tcggtttaaa
    26041 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtgc gcaggcgtcg
    26101 ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    26161 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacagatat
    26221 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    26281 gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca gccttccggc
    26341 ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    26401 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
    26461 tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg ctgggccaaa
    26521 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa tggcagacaa
    26581 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc aggtattttt
    26641 acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta ctttgcccgc
    26701 aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc atgccgcgtg
    26761 ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc ccatatcaat
    26821 aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt tcaataagaa
    26881 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    26941 cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac cggaagctgg
    27001 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg aatatcaatc
    27061 gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
    27121 gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
    27181 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
    27241 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
    27301 tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg atagccttca
    27361 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    27421 agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt ctgttgtgcg
    27481 gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt gtggtgatcg
    27541 accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt tctgcgtttc
    27601 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag
    27661 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    27721 agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    27781 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
    27841 aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc ggtgacgcgt
    27901 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaatgcg cccgacaacg
    27961 cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat taccgggcgg
    28021 ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggtatgtgga
    28081 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    28141 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
    28201 aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg cgcgtcttat
    28261 caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta cgccgcatcc
    28321 ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc attttcattt
    28381 tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt gacagcacaa
    28441 tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc atccccagcg
    28501 ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc ggcaacgaga
    28561 gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca agaaccggtt
    28621 taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg aaaccgagcg
    28681 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
    28741 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
    28801 caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc agcaccggaa
    28861 taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac accaccatca
    28921 gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg tagaagttaa
    28981 cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac acgcgggtga
    29041 tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg ttgtatcccc
    29101 ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg gtgttgagat
    29161 agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt gccacagacg
    29221 ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca aacagcagtg
    29281 ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
    29341 gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
    29401 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
    29461 ccacaaacgc cagcgacaat gttttaatca acggtgccaa gtccgggtta ttcagcacgt
    29521 cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc agcccgacgt
    29581 tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga ataatcgagt
    29641 tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
    29701 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
    29761 ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg gttttttcac
    29821 gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt caggaaaacg
    29881 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
    29941 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
    30001 gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg gcgatactgt
    30061 tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
    30121 ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc cactttagtg
    30181 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
    30241 gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt ctggcggaaa
    30301 ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag caccggggag
    30361 atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag caggcggtta
    30421 acccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat ctcttcgaga
    30481 cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga acaggtggta
    30541 tccgccagtt gatggaccag ttttttcact cgtgcgccgt cgcacatttg catcgcgata
    30601 tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag gttacccgcc
    30661 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
    30721 aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa atcccccact
    30781 acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc aatgcgaata
    30841 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
    30901 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaag
    30961 gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg ccatgagcga
    31021 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
    31081 aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag cccggcaaac
    31141 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
    31201 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
    31261 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
    31321 tccatcagcg gcacatcacc gcgcgattcc acattcaggc gcaccaccgg ttcggtattg
    31381 gaggtgcgca ggttaaagca ccagtcggca aaggtcatgc tgatgccatc gctgcgatcc
    31441 accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    31501 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgcaccagt
    31561 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    31621 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    31681 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacattacc
    31741 ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aagagggacg cggatcatgg
    31801 atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc gacaatgtag
    31861 tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa atcgccatca
    31921 aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc gtcgcgacat
    31981 tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt gttgtgtact
    32041 ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc gtccaccacc
    32101 ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag atttttgaca
    32161 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg ctgatagcga
    32221 ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg ctggacgtcg
    32281 cgcagtccgg tatcaccgct gatcgtgcga gccccctcgc gcaccagctt catgccgttg
    32341 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    32401 gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc gacgcccgca
    32461 tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg gacatcaccg
    32521 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    32581 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    32641 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    32701 tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    32761 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    32821 acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    32881 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    32941 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    33001 agtttgatat caccatcaat ggtgactttt gccgttcccg cgaccactac ccagtgttcc
    33061 gcgcggtgat ggtgcatctg taccgacaaa ccctcgcccg gtttcacggt gatgcgtttc
    33121 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atacacttcg
    33181 cgatgcaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    33241 tcctgtaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac cactaccaga
    33301 tctttcaccc cgacggtggt gaccaggcct gattcggcat acacatagct gttttcagtt
    33361 ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    33421 cataatgaag accaggaacc aacatcgctc cagcccgcat ccatcggcac cacaacagca
    33481 tctgccgtag gttccatgac cgcgtaatcc accgactctt ccgggcaggc gagaaacgct
    33541 tcttcatcca cacgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    33601 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    33661 aggaacatgc cgctgttcca gtaatattcg ccgcttgcta cataggcctg ggcggtttcc
    33721 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg ctcacccaca
    33781 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacaatgcca
    33841 aaggtcacca gcttgcccgc ttcggcataa ggcatggcat tacgcacggc ggcacggaac
    33901 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    33961 gggctatgac gttttgccgc cagcgccgcc agtgcaatgg caggtgcagt gttacgccct
    34021 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    34081 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    34141 cggcagatgg tggtttgcag catggtgaga tcgcctttca ggcataaaaa ctgcttgggg
    34201 taaagtacgc gggaaagcgg ccataaacgg ctaccggagc cacctgccat cacaactgga
    34261 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    34321 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttatgt ttgggcagca
    34381 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttct acgcaaaccg
    34441 caatacccgg aaaggtttcg caaagctgcc ccagttctgt gtgggcctct gcggtaatca
    34501 ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg gcatctgccg
    34561 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgca ggtaaagcgt
    34621 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    34681 ccagccgcgc tttgccgccg ctctgcccga caatggcaaa aatcagcggt tcatcgcgca
    34741 ggagatcggc agcttcaata acgttttcca gcccctgctt ttcaccaata ttgccggagt
    34801 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    34861 cgtctgcaac atgctgaaaa cgggcaattt ccgaccagtt ggggaagaag atgacgttat
    34921 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggaaacgt
    34981 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    35041 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    35101 gcagcacggt acgcgcacca gagagcttcg ccagcaggcg cattcccggc gtgcaaaaca
    35161 gcgttggcac tacgccaata atgcgatccg gcttccagcg acgttgcgcc atcagtggaa
    35221 agaaactgct gacggcaaaa ctgccgagat gcaacaagcg tttcagggtg ctcggctgtt
    35281 ttggcacgta cagcgggcag cgccacaccg tggcagcccc ttcttctcgt ttgtagcgcc
    35341 aggcggaata gttctcaccc acctgccatt gcgggtagta aggcggtgcg gtaatgaccc
    35401 gcacctcatg accttgtgcc gccaaccatt ccaccatctc gccggtgtat ttgccgatgc
    35461 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    35521 ggcacgcttc tcagcgagaa aataggctcg gctgttagcg tgaacatcgt tactggcgag
    35581 cagcgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagctc
    35641 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    35701 ggagaagtta tcgtcataaa aatgctgcca gacaccgtaa aactggcctg ctgttatcgg
    35761 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    35821 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    35881 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggtgt
    35941 ggagcgcact acagtggcaa agtcttcctg acgtaaaaac atcattcccc ccgaaagcgg
    36001 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    36061 tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg cgtaccatcc
    36121 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    36181 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggt
    36241 tgggtattct ccagccagac ttcatgcgcc agctccatga catgaatgct cgccgccgcc
    36301 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    36361 gtgccgtcgc ctcgtggaag cgacgcagca atgctggtat cacatgggag ttactcgggt
    36421 ggaagttgtc atgcggcccg tacaggttgg tcggcatgac agtgcggtaa tcgcgtccat
    36481 actggcggtt gtaagattcg cacagtttga tcccggcgat tttggcaatg gcgtaaggct
    36541 cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc tgttttgcca
    36601 gtttcgggta gatgcaggac gatccgagaa acatcagttt gttcacgtcg ttctgatgcg
    36661 cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc gccggatagg
    36721 tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg tcaatgcgtt
    36781 cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag gttcagttca tcgcgggtac
    36841 gtaataccag ctccacatca ccgcgctgtt cgagctgccg cttgatggca gaaccgacca
    36901 tcccacaatg gccagcgata aaaatgcgtt gtttactcat gcttatgact ccagcgcgat
    36961 cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt cgaggtcatt
    37021 agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc ccagtttttc
    37081 gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttct gccggacgga agtaacgcgg
    37141 atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc cggtgacgga
    37201 aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga tgcccagctg
    37261 tgctgccgcc atttccacaa actgacgtac ggagtactga acgccggtcg cgataacgaa
    37321 atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt ctttggcgtg
    37381 gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca gcccctgggc
    37441 gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga
    37501 ttcatggttg aagagaattc cgttacaagc gtacatgccg taggattcgc ggtagttaac
    37561 ggtgatccag taggcgtaca gtttggcgac cgcatacgga gatcgcgggt agaacggcgt
    37621 ggtttctgtc tgcggaattt cctgcaccag accatacagt tcagaggtgg aagcctgata
    37681 gaaacgagtt ttcttttcca gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc
    37741 catcgcgtcg acgtcagcgg tatattctgg tgactcaaaa gagaccgcaa cgtggctcat
    37801 tgcgcctagg ttgtacactt catccggctg tacttcacgc aaaatgcgcg tcaggttaga
    37861 ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg
    37921 ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac
    37981 ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc cggttacacc
    38041 ggtgatgaga gcgacttttg acatgtctta ttcctctgta tttttgaatt tattcagttt
    38101 caacgcgttc gcgtatcacc actgcgggat tcccccggca aaccacattt gccggaagcg
    38161 atttaaaaac actgcttcgt gcacccacga cggtgccgtc gccgattgtg acgcctgggg
    38221 caacaaagac atcggttgcc agccagcatt tctcgccaat cacaataggc gtggcgttaa
    38281 tggtgaaatg ttgacttgca tggtcgtggc taccggtgca taaataactt ttttgcgata
    38341 tcaccgaatg tgcgccaatg gttatttcac cgagggtata taaattgacg tcatcgccaa
    38401 cccacgcgta atcacctaag gttaatttcc acggataggt aatttttact gacggacgaa
    38461 taactacgtt ttttcctatt tttgctccga ataaacgtaa taaaaaagcc cgccagcgat
    38521 acaatacttg tggcgaccag gcaaatattg ttgcctgtac tgcccaccat aattgcactt
    38581 taatagcgtt gccgccccgg aaccctttcg gcaccgagaa accgcttaaa tcttgcattg
    38641 gttttcctta tattcagact ttgttatata aggctttagt cttacctgtc gtacgttggc
    38701 gtaaatgcca ggataattct gcccagaagc caggtacatg taatatctgc cgttggactt
    38761 ttttggcgtc agcac
//
LOCUS       DAJVRL010000028        37053 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVRL010000028 DAJVRL010000000
VERSION     DAJVRL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.028"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..175)
                     /locus_tag="OQN02_001455"
     CDS             complement(<1..175)
                     /locus_tag="OQN02_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS1642306.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OQN02_001456"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OQN02_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1642307.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OQN02_001457"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OQN02_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1642308.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OQN02_001458"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OQN02_001458"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1642309.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OQN02_001459"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OQN02_001459"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1642310.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OQN02_001460"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OQN02_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1642311.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OQN02_001461"
     CDS             complement(5179..5421)
                     /locus_tag="OQN02_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1642312.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OQN02_001462"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OQN02_001462"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1642313.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OQN02_001463"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OQN02_001463"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1642314.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OQN02_001464"
     CDS             complement(8338..9312)
                     /locus_tag="OQN02_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1642315.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OQN02_001465"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OQN02_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1642316.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OQN02_001466"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OQN02_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1642317.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OQN02_001467"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OQN02_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1642318.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OQN02_001468"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OQN02_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1642319.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OQN02_001469"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OQN02_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1642320.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OQN02_001470"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OQN02_001470"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1642321.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OQN02_001471"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OQN02_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1642322.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OQN02_001472"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OQN02_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1642323.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OQN02_001473"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OQN02_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCS1642324.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OQN02_001474"
     CDS             23179..23382
                     /locus_tag="OQN02_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1642325.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OQN02_001475"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OQN02_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS1642326.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OQN02_001476"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OQN02_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1642327.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OQN02_001477"
     CDS             26387..26659
                     /locus_tag="OQN02_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1642328.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OQN02_001478"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OQN02_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1642329.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OQN02_001479"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OQN02_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1642330.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OQN02_001480"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OQN02_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1642331.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OQN02_001481"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OQN02_001481"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1642332.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OQN02_001482"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="OQN02_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="OQN02_001483"
     CDS             complement(31906..32304)
                     /locus_tag="OQN02_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1642333.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /gene="zapE"
                     /locus_tag="OQN02_001484"
     CDS             32498..33625
                     /gene="zapE"
                     /locus_tag="OQN02_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1642334.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="OQN02_001485"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="OQN02_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1642335.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="OQN02_001486"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="OQN02_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1642336.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="OQN02_001487"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="OQN02_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1642337.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="OQN02_001488"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="OQN02_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1642338.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..>37053)
                     /gene="dcuC"
                     /locus_tag="OQN02_001489"
     CDS             complement(36260..>37053)
                     /gene="dcuC"
                     /locus_tag="OQN02_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1642339.1"
                     /translation="FFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTL
                     DNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHN
                     LRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIG
                     VMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAV
                     SPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8956 a         9255 c         9760 g         9082 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccataccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgtttgc cgatgtagcg
     6121 acgcagttct ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg tctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 aggcaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacttggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 taggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acagcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttccccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgt tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgacttcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agt
//
LOCUS       DAJVRL010000029        36832 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVRL010000029 DAJVRL010000000
VERSION     DAJVRL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.029"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..247
                     /locus_tag="OQN02_001490"
     CDS             <1..247
                     /locus_tag="OQN02_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1642340.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OQN02_001491"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OQN02_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1642341.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OQN02_001492"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OQN02_001492"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1642342.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="OQN02_001493"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="OQN02_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCS1642343.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="OQN02_001494"
     CDS             complement(3258..3542)
                     /locus_tag="OQN02_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642344.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OQN02_001495"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OQN02_001495"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1642345.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="OQN02_001496"
     CDS             complement(4443..5651)
                     /locus_tag="OQN02_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1642346.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="OQN02_001497"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="OQN02_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1642347.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="OQN02_001498"
     CDS             complement(6446..8131)
                     /locus_tag="OQN02_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1642348.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="OQN02_001499"
     CDS             8401..8778
                     /locus_tag="OQN02_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1642349.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OQN02_001500"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OQN02_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1642350.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="OQN02_001501"
     CDS             9225..9512
                     /locus_tag="OQN02_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1642351.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="OQN02_001502"
                     /pseudo
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="OQN02_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="OQN02_001503"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="OQN02_001503"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1642352.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="OQN02_001504"
     CDS             11269..11745
                     /locus_tag="OQN02_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1642353.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="OQN02_001505"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="OQN02_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1642354.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="OQN02_001506"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="OQN02_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1642355.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="OQN02_001507"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="OQN02_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1642356.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="OQN02_001508"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="OQN02_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1642357.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="OQN02_001509"
     CDS             16302..16790
                     /locus_tag="OQN02_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1642358.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="OQN02_001510"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="OQN02_001510"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1642359.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OQN02_001511"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OQN02_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1642360.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OQN02_001512"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OQN02_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1642361.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OQN02_001513"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OQN02_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1642362.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OQN02_001514"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OQN02_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1642363.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OQN02_001515"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OQN02_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1642364.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="OQN02_001516"
     CDS             complement(22242..22757)
                     /locus_tag="OQN02_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1642365.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="OQN02_001517"
     CDS             23406..25175
                     /locus_tag="OQN02_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1642366.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="OQN02_001518"
     CDS             25386..25709
                     /locus_tag="OQN02_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1642367.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="OQN02_001519"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="OQN02_001519"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1642368.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="OQN02_001520"
     CDS             complement(26533..27546)
                     /locus_tag="OQN02_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1642369.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="OQN02_001521"
     CDS             complement(27645..29075)
                     /locus_tag="OQN02_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1642370.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OQN02_001522"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OQN02_001522"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1642371.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OQN02_001523"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OQN02_001523"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1642372.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OQN02_001524"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OQN02_001524"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1642373.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OQN02_001525"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OQN02_001525"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1642374.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OQN02_001526"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OQN02_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1642375.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OQN02_001527"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OQN02_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCS1642376.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9213 a         9156 c         9441 g         9022 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgagtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgctgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaattggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata attgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ttggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt ga
//
LOCUS       DAJVRL010000030        35828 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVRL010000030 DAJVRL010000000
VERSION     DAJVRL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.030"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..699)
                     /locus_tag="OQN02_001528"
     CDS             complement(<1..699)
                     /locus_tag="OQN02_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1642377.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVR"
     gene            complement(846..922)
                     /locus_tag="OQN02_001529"
     tRNA            complement(846..922)
                     /locus_tag="OQN02_001529"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(886..888),aa:Pro,seq:tgg)
     gene            complement(965..1051)
                     /locus_tag="OQN02_001530"
     tRNA            complement(965..1051)
                     /locus_tag="OQN02_001530"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1015..1017),aa:Leu,seq:cag)
     gene            complement(1072..1147)
                     /locus_tag="OQN02_001531"
     tRNA            complement(1072..1147)
                     /locus_tag="OQN02_001531"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1112..1114),aa:His,seq:gtg)
     gene            complement(1206..1282)
                     /locus_tag="OQN02_001532"
     tRNA            complement(1206..1282)
                     /locus_tag="OQN02_001532"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1246..1248),aa:Arg,seq:ccg)
     gene            complement(1385..2770)
                     /locus_tag="OQN02_001533"
     CDS             complement(1385..2770)
                     /locus_tag="OQN02_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1642378.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(2961..3701)
                     /gene="rffM"
                     /locus_tag="OQN02_001534"
     CDS             complement(2961..3701)
                     /gene="rffM"
                     /locus_tag="OQN02_001534"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1642379.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(3704..5056)
                     /gene="wzyE"
                     /locus_tag="OQN02_001535"
     CDS             complement(3704..5056)
                     /gene="wzyE"
                     /locus_tag="OQN02_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1642380.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5053..6132)
                     /gene="rffT"
                     /locus_tag="OQN02_001536"
                     /gene_synonym="wecF"
     CDS             complement(5053..6132)
                     /gene="rffT"
                     /locus_tag="OQN02_001536"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1642381.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6129..7379)
                     /gene="wzxE"
                     /locus_tag="OQN02_001537"
     CDS             complement(6129..7379)
                     /gene="wzxE"
                     /locus_tag="OQN02_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1642382.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(7381..8511)
                     /gene="rffA"
                     /locus_tag="OQN02_001538"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7381..8511)
                     /gene="rffA"
                     /locus_tag="OQN02_001538"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1642383.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(8516..9190)
                     /gene="rffC"
                     /locus_tag="OQN02_001539"
                     /gene_synonym="wecD"
     CDS             complement(8516..9190)
                     /gene="rffC"
                     /locus_tag="OQN02_001539"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1642384.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9168..10049)
                     /gene="rfbA"
                     /locus_tag="OQN02_001540"
     CDS             complement(9168..10049)
                     /gene="rfbA"
                     /locus_tag="OQN02_001540"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1642385.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10068..11135)
                     /gene="rffG"
                     /locus_tag="OQN02_001541"
     CDS             complement(10068..11135)
                     /gene="rffG"
                     /locus_tag="OQN02_001541"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1642386.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11135..12397)
                     /gene="wecC"
                     /locus_tag="OQN02_001542"
     CDS             complement(11135..12397)
                     /gene="wecC"
                     /locus_tag="OQN02_001542"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1642387.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(12394..13524)
                     /gene="wecB"
                     /locus_tag="OQN02_001543"
     CDS             complement(12394..13524)
                     /gene="wecB"
                     /locus_tag="OQN02_001543"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1642388.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(13580..14626)
                     /gene="wzzE"
                     /locus_tag="OQN02_001544"
     CDS             complement(13580..14626)
                     /gene="wzzE"
                     /locus_tag="OQN02_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1642389.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(14638..15741)
                     /gene="wecA"
                     /locus_tag="OQN02_001545"
     CDS             complement(14638..15741)
                     /gene="wecA"
                     /locus_tag="OQN02_001545"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1642390.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(15981..17240)
                     /gene="rho"
                     /locus_tag="OQN02_001546"
     CDS             complement(15981..17240)
                     /gene="rho"
                     /locus_tag="OQN02_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1642391.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17325..17426)
                     /gene="rhoL"
                     /locus_tag="OQN02_001547"
     CDS             complement(17325..17426)
                     /gene="rhoL"
                     /locus_tag="OQN02_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1642392.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(17567..17896)
                     /gene="trxA"
                     /locus_tag="OQN02_001548"
     CDS             complement(17567..17896)
                     /gene="trxA"
                     /locus_tag="OQN02_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1642393.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18027..19292
                     /gene="rhlB"
                     /locus_tag="OQN02_001549"
     CDS             18027..19292
                     /gene="rhlB"
                     /locus_tag="OQN02_001549"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1642394.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            19426..20910
                     /gene="gppA"
                     /locus_tag="OQN02_001550"
     CDS             19426..20910
                     /gene="gppA"
                     /locus_tag="OQN02_001550"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1642395.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(20957..22978)
                     /gene="rep"
                     /locus_tag="OQN02_001551"
     CDS             complement(20957..22978)
                     /gene="rep"
                     /locus_tag="OQN02_001551"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1642396.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23065..23346
                     /gene="ppiC"
                     /locus_tag="OQN02_001552"
     CDS             23065..23346
                     /gene="ppiC"
                     /locus_tag="OQN02_001552"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1642397.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23392..24867)
                     /gene="ilvC"
                     /locus_tag="OQN02_001553"
     CDS             complement(23392..24867)
                     /gene="ilvC"
                     /locus_tag="OQN02_001553"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1642398.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25017..25907
                     /gene="ilvY"
                     /locus_tag="OQN02_001554"
     CDS             25017..25907
                     /gene="ilvY"
                     /locus_tag="OQN02_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1642399.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(25904..27448)
                     /gene="ilvA"
                     /locus_tag="OQN02_001555"
     CDS             complement(25904..27448)
                     /gene="ilvA"
                     /locus_tag="OQN02_001555"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1642400.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27451..29301)
                     /gene="ilvD"
                     /locus_tag="OQN02_001556"
     CDS             complement(27451..29301)
                     /gene="ilvD"
                     /locus_tag="OQN02_001556"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1642401.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(29366..30295)
                     /gene="ilvE"
                     /locus_tag="OQN02_001557"
     CDS             complement(29366..30295)
                     /gene="ilvE"
                     /locus_tag="OQN02_001557"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1642402.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30315..30578)
                     /gene="ilvM"
                     /locus_tag="OQN02_001558"
     CDS             complement(30315..30578)
                     /gene="ilvM"
                     /locus_tag="OQN02_001558"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1642403.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30575..32221)
                     /locus_tag="OQN02_001559"
     CDS             complement(30575..32221)
                     /locus_tag="OQN02_001559"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1642404.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32224..32274)
                     /locus_tag="OQN02_001560"
     CDS             complement(32224..32274)
                     /locus_tag="OQN02_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642405.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32360..32458)
                     /gene="ilvL"
                     /locus_tag="OQN02_001561"
     CDS             complement(32360..32458)
                     /gene="ilvL"
                     /locus_tag="OQN02_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1642406.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32810..34330
                     /locus_tag="OQN02_001562"
     CDS             32810..34330
                     /locus_tag="OQN02_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCS1642407.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(34355..34693)
                     /gene="maoP"
                     /locus_tag="OQN02_001563"
     CDS             complement(34355..34693)
                     /gene="maoP"
                     /locus_tag="OQN02_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1642408.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34812..35651
                     /gene="hdfR"
                     /locus_tag="OQN02_001564"
     CDS             34812..35651
                     /gene="hdfR"
                     /locus_tag="OQN02_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1642409.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(35747..35822)
                     /locus_tag="OQN02_001565"
     tRNA            complement(35747..35822)
                     /locus_tag="OQN02_001565"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35787..35789),aa:Trp,seq:cca)
BASE COUNT         8737 a         9800 c         9043 g         8248 t
ORIGIN      
        1 gcgcacagac cattccgtca gttccccctg aatatcacca ggcgcatgta gtttcagtcc
       61 atcgcggaca gctgtttcat cagccaggcg ctcgaccacc ggaataaact ggatgaattc
      121 gactcccgca tcgcacaaaa aatcataaac ctgtaacggt tgctgcgcgc tggtgcggtt
      181 gacgcagacc agcacgttat agtcgacatg atgtttttgc aggagcgtca gggcacgcat
      241 caccagttta tgcgtgggtc tgccgccttt ggtcacgcga tattgattgt ggatctcagc
      301 cggaccatcc agcgataacc caacaagaaa attattttct gccagaaatg cacaccattc
      361 gtcatcgagc agcacgccgt tagtctggaa actgttactt atcttcctgc cagcaccata
      421 tttcgcctgt agcgccacgg cacggcggta aaactccagc ccgagtagcg ttggttcgcc
      481 gccctgccag gtaaaagcca cttcgttttg cagttcgctg gcggcgatat agtgacggat
      541 atacgcttcc agcgtgtcat cgtccatatg cgtgactggc ttttcgcggt aaagggattg
      601 tttttcgaga taaaaacagt agtcacagtt cagattacaa tcggaaccgc tcggtttcgc
      661 tatcacatga aaagcacgcg ttggaacctg ttgcagcatg gtcgctcctt gcctgaagtc
      721 atccttcaat acgttaaggg cgaaatcata ccgtggggca atgagttatt acgggcgttt
      781 attgcgaagg gagggtgacg aaatgcacag aaaacaaaaa accccgccga agcggggttc
      841 aaaattggtc ggcgagagag gattcgaacc tccgacccac tggtcccaaa ccagttgcgc
      901 taccaagctg cgctactcgc cgaaatactg ctttttgaat ttttagttca attctttaaa
      961 gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt aaggacacta acacctgaag
     1021 ctagcgcgtc taccaattcc gccaccttcg cattgtcaca acttctaata atggggtggc
     1081 taatgggatt cgaacccacg acaactggaa tcacaatcca gggctctacc aactgagcta
     1141 tagccaccac tacaaatctt gttacgcggt attactacca ccgcagctca agcgccggta
     1201 ctaaatggcg cgcccgacag gattcgaacc tgagacctct gcctccggag ggcagcgctc
     1261 tatccagctg agctacgggc gcttagcgcc gttgcggggg tggataatac ggacttccca
     1321 cccctctgtc tagtcccttt ttaaataaat tgctcgtttg gttacggttt gtgcgttttg
     1381 ctgcttattc ctccagttta tgcgctttac cgtggcgatt aagcccaaaa actttataaa
     1441 tcgccgtcac cgccagcata aagatgatgc cgacaaacag cgacatacgc gtatcttcat
     1501 taaagtacat gccgatcaaa acgcaaatca ggaatgccat tgttacgtag ttggcccacg
     1561 ggaacaggat tgagcggaac ggatggctgg caatcgccgc tttatgtgca cgccgaaaac
     1621 gcagctggct tatcaatatc acaaaccatg gcaccatccc cggaagcacg ctggcactgt
     1681 agacgtagac aaacacacgc tgcggattgg gaatgatgta gttcaggcat gagccaatta
     1741 gcagaatagc aatagatact gccacacccg caaccggtac gccgtgacgg gaaacttttg
     1801 ccattgccgc cggtaactga cggtttttcg ccagtgcgta gagcatacgt ccgcaactgt
     1861 acatgccgct gttacagcca gagagcgcag ccgtcagcac cacaaagttg ataatgcccg
     1921 ccgctgcggt aataccgatt ttggcaaaag tcagtacgaa cgggctgccg ttgctgccaa
     1981 tttcattcca cgggaagatg gtgacgataa cgaaaatcgc gcctacgtag aaaatcagga
     2041 tccgccacag cactttgcct acggcactgc gcagcgtcac ctgcggattc ttcgcttcac
     2101 cggcagtaat gccaatcagc tccacgccct ggtaggacgc caccacaata cacagagcgg
     2161 tcaggaaccc tttccagcca cccgcaaaga aaccgccatg ctctgtgaga ttgctaaaac
     2221 caatcgactg cccgccattg ccaaagccaa agaaaatcac gcccaggcca atgacaatca
     2281 tcacgataat cgtggtgact ttgatcatcg cgaaccagaa ctcgatttcg ccgtacaacc
     2341 gcaccgccgc cagattcgcc aacgccacca gcgccaccgc gatcaatgcg ggtatccact
     2401 gcgccatctc cggggaccaa aactggacat aaacgccaat ggcggtgatt tctgagatcc
     2461 ccaccgccat ccacataaac cagtaagacc aggcggtgag atagccaaaa aacgggctca
     2521 tataacgatg cgcataaacg gcgaacgaac cggtaaccgg ttcgaggaac aacatttcgc
     2581 ccattgaacg catgatgaaa aaaacgaaca gcccggcgat gatataggcc aacaatacgg
     2641 atggcccggc ccatttcagg gtactggcgg cccccataaa caggccgacg ccaatggtgc
     2701 cccccagggc gatgagttcg atatgtcgag cttccagccc acgctgtagc tctggtttgt
     2761 tatctgccat aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg gttatcgtta
     2821 tgggtacatc gagtgttgcg aatgttttcg taattcagga gaaatggcaa ataaagcatt
     2881 aaaaatttga atgctttgtg taataaaaaa gcagacaggc gacgaagtga ccactccgtc
     2941 gctttacaaa gagaggaaaa tcagaggttg ccggtgtagt gccagcgtaa ataacgcagc
     3001 aaacgaagct gacgcttaat gcggctcggc tgcgaaagca ggcggtagag ccactccagt
     3061 cccagcgttt gccagatttt cggtgcgcgt tttacgtgac cggtgaaaac atcgtaagtc
     3121 ccgccaacgc ccatatacag cgcatctgga tgcaccagac ggcagtcgcg catgaagatc
     3181 tcctgctttg gcgatcccat cgcaacggtg acgatttgcg ctccgctggc atgaatgcgt
     3241 tcaaacagcg cctgacgctg ctcgggttta aaataaccat cctgactgcc aacgatattc
     3301 acattccatt ggttgcgcag tttagcttca gtttgcgcca gcacttcagg tttaccgccc
     3361 acaagaaata ccggcgtccc ttctttgcct gcgcgcgcca tcagctcttc ccagagatcg
     3421 gcaccggcaa cgcgggaaac ctgcgcctgc gggtactttt tacgtactga acgtacaacg
     3481 ctgatgccat ccgcatattt aaattcggca gcgttaatta actccctgac ctcggcgtta
     3541 tcttcaatgg tcagcatttt ttcggcatta atggcaacca gcgttccctg cttaagctgc
     3601 ccgtcagcaa acagataatc gagggcatgc tgcatatcac gccaaccaat caactgtaag
     3661 ccacgcagcg tataggtcgg tgccgtggtg ttgttattca ttgttatcct tcaacctgcg
     3721 tccggagcga tgattttgta cgtttatgaa tgagtccggc gctttcaaaa agccagtaca
     3781 acagttttgc gatcatcaga catgcgccga agaccacgat aaaaaagact acgcgtgaga
     3841 caaacgaatc cagcccttca cgtgccagca cgatcatatt gaagatcgcc ccaaagcaga
     3901 aactgtgcaa tatcgcagcc ttatagcgat tagtctcgcg gttgcccagc tcatacagcc
     3961 agtcgaacca tttgatgatc agtccaacca caatcgcccc gagtgggatg aacaacgcgc
     4021 cgcccatcac caccagtgag cctataagcg taggcgagat cgccagtccg gagtggttat
     4081 tcagtacttc ccaggtaaag taattggctg agttcagcac catactcggg cgccccggcc
     4141 acagccagga agggataaag acatagaaat cgcggacaat tggagccagg ccctggaagt
     4201 cgatgttgtc gtagttctgc aacagcaacg ccagattctc ccacggtgaa aaggtgtcgc
     4261 gagtgagata gagaaacgta tagaacgctt catcgccgct cacattcatt ccatagcgtt
     4321 ttaacgccag ccagaacatg ccaacaatcc ccagcacgcc cgccgccgcc agcatccaca
     4381 acgaaatcca gccgcgaata atgccaataa acaggaagat agcgaatgcg atgatgatat
     4441 tggcgcgagt gccgccgaca atcatataag tcagcaagcc aaaggcgacc gtgctgacga
     4501 ggaaaaacag ccacgctttg ctgtcctggc gcagaaagta gaccaccagc atcgccggga
     4561 tgaaaaagta aaagaagcgt tttaacgcca cgccggagac ttcactggaa aagatctgac
     4621 tgtaggagtt aagccggaac agcaaaaagc cgttgtgcat gaagaagatg ccgacgctta
     4681 ccagcgcgat acccatcagg atcacccacg taagattggt ctccacgcgg ttcatggtaa
     4741 acagcggacg gcgcggtaca tcagcaacgc gtttgcgtag gcgggttttg taggtgacat
     4801 agtaaaccgc gtagaagcag cccgcagaaa gcaacacctg caacaagatt tctggcggcg
     4861 cgacaccaac atcaaagcga aataccagca cgctggtcag cgggaagccg aagaaaaagg
     4921 tgagcaaaaa cagcaatgaa aagaagacat tgaagttaaa gcgcacacgg cgaaactcaa
     4981 accaggtcag cgtggcaata aacagcgtgc agagcagcca gaccacaaac aggccactga
     5041 attgcagcag actcatgcga cctccccggc ggcaatcgcc aacgcccgct gccagccttg
     5101 tagatagtta gggctaaaga aggcaatggt gtttttatcc gccgacgcca actggcgctg
     5161 cgcttcacgc acaatatcct cgttgagatc gtcggtagta aacagcactg gcaaatgctg
     5221 ttccgtcata tcctgccaga acggattttc cctgttaagc acacaaggaa tgcccgcctg
     5281 aatcagtaag caaagcgtac caatgccctg ctggcgggca aaaataaagt aaccaagatc
     5341 gcactgacga agtagcgcca gataggcgtc aaattccagt ttttcgctca aaatttgcag
     5401 attttcttcg ctgaataact ccagccccgc ctgacgaact tcctcaatgt acgcttcgtt
     5461 attaggcgga tatcccatcg gcaccaccac ttttaccgta tcgccaaatt gctgatgaac
     5521 ggcacgtaaa gcggcgacat gctcattgct gcggtcgccg gagttcccca ccagaatggt
     5581 catttttcct tcacgttgcc gatcgttcgc catcatattg agagaagggt ccatccgcgt
     5641 cgggaagtac agcagttctc cccgtacctt tgggtgcgtt ttggcaaaaa agctcaaatc
     5701 gccgcgggtg gcaaatacac agccgactcg cttttgcgcc aggcgacgta gtgggtaaaa
     5761 aagcttatat ctcaagccac tggaaagctc atacaggtct gccccccaga tatgccagaa
     5821 aaactggctg ggcttaatgc caccactcag cagagccagc cacagtgtgg gattgaactg
     5881 accgtggaaa aaaaatcgct gctgacggtt agcttttgct ttcgcgatga ccgcttccgc
     5941 cagcgatttt ttcccaggga aaaattgcac agaaagcgcc ggacagctat cacttaagcc
     6001 gtcgtccttg ccaacaacca taaactcgcg cgcgtgctcg ctcgtcgcgg ccagcgcgtc
     6061 attgaaaaac cgcaaaacgg ttcggttatg gtgagggata tccgatccca gtacgtgaat
     6121 cagtacagtc atgcccgcct acgccagagt aaaaacacgc cacaacaaag agaaaaatag
     6181 acgatataag ttgccatata tgcctgcgcc gcgcccagtg caccatgcgc agggattagc
     6241 cagtgggcaa ataccatcaa taaagtgaac tggctgactt ccgccagaat ataaaaccgc
     6301 agtgacgctt tggcgatcac cagataacca aagacataag cgcccacttt taacacatca
     6361 cccactaact gccaggcaaa gagatcgcgc atagcggtaa atttattcga caacagcaac
     6421 cagatagcaa aatcacgcag caaccagacg gtaaaactcg ctgccgccac tgccggtaag
     6481 acgaatttca gcgatttaac cacttcccgg gtgatatcgc gcttttccgt tagccgcgac
     6541 aacgtgggca gcaaatatac gctgaacgat gccgtaataa attgcaggta ggcatcggaa
     6601 atactgctca ccccttgcca gatccccact tcatcccagc tatactgcgc cgccagcagt
     6661 ttacgcatca tgatgtaagc aacaggcaag gtcaccgacg taatcaacgc catgagcgta
     6721 aatttgctca actgccctgc cagaccgtta tcccagctgg gtttcagata gcttaacggg
     6781 atgacaccac gtttaatcaa catgatggcg gcaggaatta ccaccagcgc gggaatcagc
     6841 gccagaccca gcaacgcccc ttcataaccg cccaaacggt aactgacgta gtacgcgaga
     6901 acgccaatca agctgccgac aatcagcgat aacgcattac ctgcggcatc gcgaaagcct
     6961 ttcatcagcg ccagtaacag gttgccccag gcgatcccca tttgcaccag cgccactaaa
     7021 cgcaccagcc cctgatagtc ggtattacca aacaatccct ggctgattgg cgcagctgcc
     7081 agcacaaaaa ccagcgccat cagcgtagag aaaccaagta ccatcgctga tgaagtgccg
     7141 accacgcggc gcagctgttg cggattatca tggtactggg caacgtattt ggttacaccg
     7201 ttaaagatgc cagccccggc aagcacgccg agcacggtaa tcaactggcg gaaatttgcc
     7261 gccagcccaa gccccgccgg accaaatgac accgccagca acttaccgac cagtaacccg
     7321 gcaccaatct tgaccagtgt actggccgcc gtccacaagg acgcttttgc caacgacata
     7381 tcaggagaag tagttcaaca aagtcgcaat taccgtacgc tgattgacgg gcgacaggtt
     7441 gtagaacagc ggcaggcgca gcaggcgctc gctctctttg gtggtgtagc gatcttcacc
     7501 gtggaactca ccaaagtgtt cccccgcagg gcaaccgtgc agcggaatgt aatgaaacac
     7561 cgccatgatt tccgcttctt tcagaaagtt aatcaacgcg ctccggtcat caatatcccg
     7621 cagtttaatg tagaacatat gcgcgttctg cacgcagcca tcgggaatcg acggcagctc
     7681 gatacgcccg gctttcgcca gaggcgctaa cgtatcgtag tagttttgcc acagcgccag
     7741 acgttgctgg ttgatacgat ccgctgcttc cagttgcgcc cacaggtacg cagcttgcag
     7801 atcggacatc aaatagctgg agccaatatc gcgccaggta tatttatcga cctgaccacg
     7861 gaagaactgg ctgcggttag tgcccttttc acggatgatc tcggctcgtt cgattaacgc
     7921 tttatcgtta atcagcgtcg cgccgccttc accacccgcc gtgtagtttt tggtttcatg
     7981 gaagctaaag cagccaatat gaccaatggt tcccagtgca cgccctttgt aagtggacat
     8041 cacgccctga gcggcatctt ccaccacaaa caaattatgc tttttcgcca acgccataat
     8101 ggtgtccatt tcgcaggcca cacccgcgta atggaccggc acgataacgc gcgttttgtc
     8161 ggtgatcgct gcttcaatca acgtttcgtc gatgttcatg gtgtccgggc gaacatccac
     8221 aaagacgatt ttcgcgccac gcagcacaaa ggcattggcg gtggagacaa aggtgtagct
     8281 cggcatgatc acttcatcgc caggctggat atcgagcagc agcgccgcca tctccagcga
     8341 agcggtgcag gacggcgtca gtaacacttt ggcgctgcca aaacgttgct ccagccactg
     8401 ctggcagcga cgggtaaaac cgccatcgcc acacagtttg ccgctaccca ttgccgactg
     8461 catatagtcg agttcggttc ccaccaccgg cggtgcgtta aatggaatca tgtgatcacc
     8521 tgtataacca gtacgcggtg ctttctacat tcgcaccact ttgtatgtat cgtttaagcg
     8581 cggcggtgtt gcccatttgg gtcgccaccc gcaaagttgt tttaccgcga gcatacgccc
     8641 agtttagcgc cgtttgcatc agctcagcac ctgcaccgcg tccagccagc aggccaattc
     8701 gcgcatctgt cgcattgagt tcccgtaaag agacatagcc gcgaatatcg ccggacgccg
     8761 cacgtagaat cagacattga tgatcaaagg tgccgcgcac ggcattttca atccactgtg
     8821 cataaaagcg accgctggcg tcaggcgcat accacggcgc acgaaaacgg ctttgcgcaa
     8881 atgcggcgct agctaactga cgtaatgcgg gaatatcggt ctcttgtgcc acgacagcac
     8941 cgctatcact gacattgttc acgggtagcg ccaaatcaac ttcaccttct accagggaga
     9001 atcccagctg ttgcagggca tccagttccc ccgtatttga tgccgcaatt ttggcctgca
     9061 cccgtgacca tggcgctaag gcgtctgacg tcaggagcgg tgcttcagac gtaatgcgca
     9121 cgatggcgct gttaacacca aagaaggcgt tttcccaggt tagtggctca atactggcgc
     9181 ggacgggcac gaagtaactc cagcagatat tggccgtagc cagttttcgc taatgaactg
     9241 gcagcacgct tcacaccctc gtcatcgagc cagccgttac gccaggcgat ctcttccagg
     9301 caggcaatct taaagccctg gcgtttttct actgtctgca caaaagtgct ggcctcaatc
     9361 aggctgtcgt gagtgccggt atccagccag gcaaatccgc gcccgagcag ttcaacggtc
     9421 aggttgcccg cctcgaggta catctggttg atggaggtaa tctccagttc accgcgctcc
     9481 gacggcttca cctgctttgc gtactccacg actttactgt cgtagaaata aagcccggtc
     9541 accgcccagt ttgacttcgg ctgttttggc ttttcttcaa gcgagatagc gcggaaattg
     9601 tcgtcaaatt ccaccacgcc aaagcgttcc gggtccatca cctgatagcc aaaaaccgtc
     9661 gccccttccg tgcgcgccgc aacatgacgc agcttcggac tgaagccctg accgaagaag
     9721 atgttatcgc ccagcaccag acaagaaggt tcaccattaa ggaaggtttc accgatgata
     9781 aaggcctgcg ccagaccgtc cgggctgggc tgttcggcat attccagctg gataccgaac
     9841 tcactaccat cgcccagcag gcgctggaaa taacctttat cttccggcgt agtgatgatg
     9901 agaatttcgc ggataccggc cagcatcagc accgacagcg gatagtaaat cattggctta
     9961 tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa tcgggtaccg
    10021 gaaccgcccg ccaggataat acccttcatg tttgcctccg ctgaaaatta gcctttcaga
    10081 cctaaacgct caccctgata gctgccgtcc tgcacctgct tccaccagct ttcattagcc
    10141 agataccact gaaccgtttt acgcattcca ctttcaaagg tttcctgcgg cagccagcca
    10201 agttcacggg caattttcga agcatcaatg gcataacgca gatcatgccc cggacggtca
    10261 gcgacaaagg tgatcaggtc acgatagtgc gccacgccgt gcggcttgtt cgaagccagt
    10321 tcttccagca gctcgcaaat ggtttccaca acatcgagat tcttacgctc gttgtgacca
    10381 ccaatattat aggtttcacc gactttcccg gtggtcgcca cgcaatacag cgcgcgggcg
    10441 tgatcttcca catacagcca gtcacggatt tgctgcccgt tgccatatac cggcagcggt
    10501 ttacccgcca gcgcgttgag gatcatcagc gggatcagtt tttccggaaa gtggtaagga
    10561 ccgtagttat tcgagcagtt ggtgatcagc gtcggcagac cgtaggtccg cagccaggcg
    10621 cgcaccagat ggtcgctgct ggcttttgac gcggaataag ggctgctcgg cgcatacggc
    10681 gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac catatacttc gtcggtggag
    10741 atatgatgaa aacggaacgc tgatttttta tcttccgtca gcgtattcca gtaagcccgc
    10801 gccgcttcaa gcaatgtata agtcccgaca atgttggttt caataaacgc tgccgggccg
    10861 tcaatagaac ggtcaacatg gctttcggct gccagatgca tgacacagtc tggctgatgc
    10921 tcagtgaata cgcgtgccag ttctgcccga tcgcagatat caactttctc aaaggcaaag
    10981 cgctcgcttt gcgcgaccgg tgccagcgac atcaggtttc cggcgtaggt cagcttatcg
    11041 accactacca ccgcatcgct cgtttcgttg atgatataac gcaccagcgc cgagccaata
    11101 aacccggcac cacctgttat cagaattttt ctcatcagcg ccagactcct ttggcatcga
    11161 cgacatactg ctgatggaca ttgtcgccat tgataacttt gaactgacta tgatcgacca
    11221 gcatcaccag cacgtctgcc gttgccagcg cctcgtcaag ctgcgccaga gtacaaagcc
    11281 cggtcagttt tttcggcaac tggtggatgt taggctcaac aaccagcgtt tcgccgctgt
    11341 gccactgggc gatcagttca gcgatttcca tcgccgggct ttcgcgcagg tcatcaatat
    11401 tcggtttaaa cgccagacca aagcaggcga ttttcagttc actggcgcgt ttatcggtag
    11461 cagcaaggca gtcggcaacc gccgctttca cctgatcgat aacccagaac ggtttgtgat
    11521 cgttaacttc gcgcgcggta cggataagcc gcgcctgctg ggggttctgt gccacgataa
    11581 accacggatc aacagcaatg cagtgaccgc ccacgccagg gccaggctga agaatattaa
    11641 cgcgagggtg acgattcgcc aggcgaatca gttcccagac gttaatcccc tgatcggcac
    11701 aaatcagcga caattcatta gcaaaagcga tattcacatc gcggaagctg ttttcggtga
    11761 gcttacacat ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg aggaaaattt
    11821 tgtacaattc gctggcgcgg gccgaacaaa ccggcgtcat accaccaatc acgcgatcgt
    11881 ttttaatcag ctcgaccata acctggcccg gtaatacacg ttccgggcag taagcaatgt
    11941 tgacgtccgc ctgctcgccc acctgctgtg ggaaagtcag atccgggcgc atctctgcta
    12001 accattctgc catcttctcg gttgacccca ccggcgaggt ggattcaagg atcaccagcg
    12061 cgcctttttt cagcactggc gcaatggagc gagcagccga ttcaacgtag gtcatatctg
    12121 gctcatgatc gcctttaaac ggcgtaggta cagcaatcag ccaggcatcc gcttcaactg
    12181 gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt ttttactacg ctcgccaaat
    12241 caggctcgac gatatggatt tcgccacgat tgatggtatc aaccgcgtgt tggttgatat
    12301 cgacaccaat cacctgtttt tgccgtgagg caaacgctgc tgctgtaggc agcccgatgt
    12361 aacccagtcc gataacagaa atggtcgcaa aactcatagt gatatccgat tattttttaa
    12421 cgcttccaga atgcgagagc atgcctgacc atcaccatac gggttatggg cgcggctcat
    12481 agcttgatat tcgttttcgt cttttaaaag acgcgtcact tcctcgacaa ttcgctgctt
    12541 atccgtgcct accagacgca ccgtacccgc cgtcaccgct tccggacgct cagtggtatc
    12601 gcgcatcacc agcaccggtt tccccagcga aggcgcttct tcctgaatgc cgcctgagtc
    12661 agtcaaaatc agccaggcgt ggttcatcag ccagacaaac ggtaaatact cctggggatc
    12721 gatcagaatg acatttttca catgccccag aatgcgattg accggctctc tgacgttcgg
    12781 gttgagatgc accggataga caatctggat gtcctggtgc gtggtggcga tgtctgccag
    12841 cgcgtggcag atttcttcaa agccacgacc gaaactctca cgcctatgac cggtcaccag
    12901 aatcatcttt ttatcggggg cgataaacgg gtaatttgcc gccagttctg aacgcagcgt
    12961 gtcgctgctc atcacctggt cacgcaccca taacagtgca tcaatgactg tattaccggt
    13021 aatgaagatt cggctatccg caacgttttc acgcagcaag ttttgccggg aagtttcggt
    13081 tggagagaag tgatacatcg ccagatgccc ggtcaatgta cggttagcct cttccggcca
    13141 cggcgaatag agagcgcccg tgcgcagacc agcctcaacg tgaccaacag gaatacgctg
    13201 ataaaacgcc gccaggctgg ttgccagcgt cgtcgtcgta tcgccgtgaa ccagcacgac
    13261 gtctggtttg aactcggcaa gaataggttt tagcccttcc agaatccgac aggttatctc
    13321 tgtcaggccc tgtcctggct gcattatgtt gagatcgtag tcaggtacaa tggaaaagag
    13381 tttcagtacc tgatcaagca tctcccgatg ctgcgcagtg acgcaaactt tagcctcaaa
    13441 aaaaggatct tttgccaacg catgcaccaa cggagccatc ttgatggctt ccgggcgcgt
    13501 accaaataca gtcagtactt tcacatcgat tctcttcgaa taagcggcga gcgcctttgc
    13561 gctcaccgca gcagtgttgc tatttcgagc aacggcgggt taatgcgaca ccagccccga
    13621 tcagcccccc gacaatgccc cacataatca tcaggaaggc acgacgtggg ctatcgcgtt
    13681 ttaccggttc ttccggcgta cgcaaatagc gataggtctg aaaacgcgga tccagggttg
    13741 gaccaacatt cagggtgttt aacatggctc gattctgatc atagtcgaga tcaaaggccg
    13801 gaccgacggc ctgtaaattt tccagtcgag cctggagcat tggacgccca agcaggaaca
    13861 tttctgaatc aggtaattcc tcggcaggca catctgtcgc actgcgcgaa atattatgct
    13921 gctcagcaat tttcagcgcc tgctcaatac tgttcatccg gcggtcgtag atggctttcg
    13981 ccacctcttc ctgacgcttc acctgagctt tcatctggat ggtgcgtgcc gcccatgcgc
    14041 ctttcagctc atcattcaga tggctggctg cacgctggct ggcaaaagca acatactgac
    14101 gtaacaggtt attagcgtca ggcgcggttt cggcaataag cttcacgctg tcattgaccg
    14161 cgcgagtaaa gtctccgggg ataaactgga tgttgttaat catttcatcc agcaacgccg
    14221 catcggcttt gctgttgccc accatccgct gtttgtaata gtcggtttgc agccagaact
    14281 ctctgcgggt atcccacgag gccagttgca taacaaactc tttataggct tcgtccatga
    14341 ccgatggttg atcggcagaa gccatgtttg aacggacatc caggttacgc aaaaattgct
    14401 gctgcgagta atatcccccc agcatattca ccgttggacg atcggtaatc gccgtcgagc
    14461 tccactcctg acgagcaaaa aaagtatacg ccagcgcgat taacgcaaac gccagcccca
    14521 tgccaataat ccatagcttc ccagcccaca aggtacgaaa caacccacga atatccagtt
    14581 cattttcagc gtcttcggcc ggtttcccag gcattggttg tgtcatcaca tcctcattta
    14641 tttggttaaa ttggggctgc caccacgatt tctacgcagt ctgcgtttta cgcgcttaat
    14701 aaagcgagca actttccagg cacgcttaat gcaatatcca tagaggaaga atgctagcaa
    14761 aaagagcacc agcatgaccc actccgggac aaaatgagaa tattctgcca gcacgccaat
    14821 ggaagcgagc agtgctgcgg caagggtaat cagcacaaac gcctgacggg aagtaaaccc
    14881 ggcacgcatg atcaaatggt gaatatgctg acggtcagga gagaatgggc tcatgccttt
    14941 acgcaggcga cggtacataa tcgccaccat atccattagc ggaatggcga ttatccacaa
    15001 agcggtaacc gggctgatgg gatgggtttt gccctgggtc gtttcgagca ggatccagat
    15061 aacggtaaaa ccaatcagcg tactgcccgc atcacccata aagactttgt agcggcgacc
    15121 caggatacca aggttaagca tgatgtatgg caggatggcg gcgatcatcg caaagcacca
    15181 gattgcgagg ctggtttgcc cgtcgaacca caaaatcata ccgattgctg caaacgagac
    15241 gcaggacaac ccgcccagca agccatcaat gccatcaacc atgttgaacg cattaatggc
    15301 cgcccagacg gcaaatagcg tcaggaagta accaaacggt ccgagcacca tctcccagga
    15361 gccaaagata taacccaggc tactgagata aagtttgccg aacaccatca taacaatgcc
    15421 aacagcggcc tgtatggtgg cacggatttt tacgctgata tcaaaacgat catccagcgc
    15481 gccaatgaaa acaagcacac cggcacaagc gagatagaga gatgcatgcg gaatatagta
    15541 atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc gaaatccccc caacgagagg
    15601 tatcaatccc tggtgacgtt tgcggaagtt tggtttatcc actaaaccga ctttttttgc
    15661 caccttacgg gcaaaaaaca gaaacagtgt cgtgaataaa aaaatactga tgagatcagt
    15721 actcactgtc agtaaattca caatgcatgc tctcagagaa aattattagc agaagtataa
    15781 ccacgaagac ctttattcag aagggaaagt ccgttccgaa cccaccagcc ctgtgcaaaa
    15841 atcaaccatt tgatatgcaa attgcataac atgccagcaa attccaggaa ttttcctaac
    15901 atcgccagcg cgacattaag attacagata taaaagcaaa acgccacgta aacacgtggc
    15961 gtttttggca taagacaaat ttatgagcgt ttcatcattt cgaagaaatc gtcattggtc
    16021 ttggtcatgg ccagtttatt aatgaggaat tccattgcat cgatttcgcc catcgggtga
    16081 atgattttgc gcaggatcca cattttctgc agttcttcct gagtcgtgag cagctcttct
    16141 ttacgggtac cggaacggtt gtagtcgata gccgggaaga cgcgtttttc agcgatctta
    16201 cgagagaggt gcagttccat gttgcctgta cctttaaact cttcgtaaat aacttcgtcc
    16261 attttagaac cggtatcgat aagcgccgtc gcgataatgg tcaggctgcc gccctcttcc
    16321 acgttacgcg ccgcaccaaa gaagcgtttc ggacgatgaa gggcattggc gtccacacca
    16381 ccggtcaaca ctttacctga cgccggaaca acagtgttgt aagcgcgcgc cagacgagtg
    16441 atggagtcga gcagaatgat aacgtctttc ttgtgctcaa ccaggcgttt ggccttctcg
    16501 atcaccattt ccgcaacctg aacgtggcga gatgcgggtt cgtcaaaggt agaagcaaca
    16561 acttcacctt ttaccagacg ctgcatctcg gttacttctt ccggacgttc gtcgatcagc
    16621 agaaccatca gcacacaatc cgggtggttg taagcaatgc tctgagcaat gttctgcagc
    16681 agcatagttt taccggcttt cggcggtgcc acaatcagac cacgctgacc acgaccgata
    16741 ggtgatgcca gatccagtac gcgagcagtt aaatcttcag tagaaccgtt accacgttcc
    16801 atacgcagac gagagtttgc gtgcagcggg gttaagttct caaagaggat tttgttgcgg
    16861 gcgttttcag gtttgtcgaa gttaacttcg ttaactttca gcagcgcaaa atagcgttca
    16921 ccttctttcg gcgggcgaat cttaccagag atggtatcac cagtgcggag gttgaaacgg
    16981 cggatttggc taggggaaac gtagatgtca tcaggaccgg cgaggtagga gctgtctgcg
    17041 gaacggagga aaccaaatcc atcctgcaat atctccagta cgccatcacc aaagatatct
    17101 tcgccactct ttgcgtgctg cttcaggatg gcaaaaataa tgtcctgctt acgcatgcga
    17161 gccaggtttt ccagccccat attttcgccg agagtgatca gctcagaaac cggcgtattc
    17221 tttaattcgg taagattcat agtggtgtga gttcttaaac ttggggtaaa tctcgaactt
    17281 aatgttgtga atggtatggc aggatcatcc atgcctgttt acggtcatcg actcatgtct
    17341 tttcgctgcc tggtcacagg agagtacgca gaactgaaac gacaagacgg attgagtgac
    17401 gagccagaaa tctgttcact tcgcatttaa gataaaacgg gaagcgttgg aaattacaag
    17461 attcaaactt aataaggtat gtttaatacg aagtcaacac taagttagca tgactcacgc
    17521 cgggcgtcca gtttttagcg acggggcacc cgaacatgaa attcccttac gccaggttag
    17581 cgtcgaggaa ctctttcaac tgacctttag acagtgcgcc cactttggtt gccgccactt
    17641 caccgttttt gaacagcagc agagtcggga taccacggat gccatatttc ggcgcagtgc
    17701 cagggttttg atcgatgttc agttttgcaa cggtcagttt gccctgatat tcgtcagcga
    17761 tttcatccag aatcggggcg atcattttgc acggaccgca ccactctgcc cagaaatcga
    17821 cgaggatcgc cccgtccgct ttgagtacat ccgtgtcaaa actgtcgtca gtcaggtgaa
    17881 taattttatc gctcatatat aactccacag gaataagcct ggcgtgttgg tttcgttgtt
    17941 ggtgtaacat taaccaacta aaggttgact ttatttcacc ggatacgctt tcgtaaagca
    18001 atagtaagct gatattctac cacactatga gcaaaacaca tttaacagaa cagaagtttt
    18061 ccgacttcgc cctgcatccg aaggttgtag aagcccttga aaaaaaaggg tttcataact
    18121 gtacgcccat tcaggcactg gcccttccgc tgacgctggc gggtcgtgac gtagccgggc
    18181 aggcgcaaac cggtaccggg aaaacgatgg cgtttcttac gtcaacgttt cattatcttc
    18241 tctctcatcc tgcgattgcc gatcgcaagg tgaatcaacc gcgtgcctta attatggcac
    18301 cgacgcgtga acttgccgtg cagatccacg ccgacgcgga accgctggcg gaagctaccg
    18361 gcctgaagct gggcctggct tacggtggtg atggctatga caaacagctg aaagtgctgg
    18421 aaagcggcgt tgacattctg attggcacca cgggtcgttt aattgactac gccaagcaga
    18481 accacattaa cctcggtgcc attcaggtag tggtactgga cgaagccgat cgcatgtacg
    18541 atctaggctt tattaaagat atccgctggc tgttccgccg tatgccgcct gcaaatcagc
    18601 gcctcaacat gctgttctcc gccacgcttt cgtaccgggt acgtgaactg gcgttcgagc
    18661 agatgaacaa tgccgaatat attgaagtgg aaccggaaca gaaaacgggc caccgtatta
    18721 aagaagagct tttctaccct tctaacgaag aaaaaatgcg tttgctgcaa acgctgatcg
    18781 aagaagagtg gccagacaga gcgattattt tcgccaatac caaacaccgt tgtgaagaga
    18841 tctggggcca cctggcggca gatggtcatc gtgtcggttt gttgacaggc gatgtcgcgc
    18901 agaaaaaacg tctgcgtatt cttgatgaat ttacccgtgg cgatctggat attctggttg
    18961 ccaccgacgt tgccgcgcgt ggtttgcata ttccggcagt gacgcacgtc tttaactacg
    19021 atttacccga tgactgtgaa gattacgttc accgtattgg tcgtacaggt cgcgcaggcg
    19081 caagcggtca ctctatcagc ctggcgtgtg aagagtatgc attgaatttg cctgctattg
    19141 agacctatat tggtcactca attccggtaa gcaaatacaa tccggacgca ttgatgaccg
    19201 atctgccaaa accgctgcgc ctcacgcgcc cgcgcacagg caatggtccg cgtcgtactg
    19261 gcgctccgcg taatcgtcgt cgttcaggtt aaataaaatg tcatactcaa aatcattcaa
    19321 actgtatcaa gatggcaaga gaatgaatcc cgatgagctt actgtagtaa gtgattcggg
    19381 tgaaagaatg cagccaacac agagacagat tgaaggatga agagtatggg ttccacctcg
    19441 tcgctgtatg cagccattga tctcggttcg aatagttttc atatgctggt tgtgcgcgag
    19501 gtggctggaa gcatccagac gctgacgcga attaaacgca aagtgcgtct ggctgctggc
    19561 ctgaatagcg aaaatgacct ctctaatgaa gcaatggagc gcggttggca atgtctgcgc
    19621 ctgtttgctg aacgtctgca agatatccct ccctcgcaaa ttcgcgttgt cgctacggcg
    19681 acattacgac ttgccgtcaa tgcgggtgat tttattgcca aagcgcagga aatcctcggt
    19741 tgtccggtac aggtgatcag cggtgaagag gaagcgcgtc tgatttatca gggcgttgct
    19801 cacaccactg gtggtgccga tcagcgcctg gtggtggata taggcggtgc cagtactgaa
    19861 ctggtaaccg gcacgggtgc acaaaccacc tcgttgttca gcctgtcgat gggctgcgtc
    19921 acctggctgg aacgctattt tgccgatcgt aatctggggc aagaaaattt tgatgctgca
    19981 gaaaaagcgg cacgcgaagt gttacgtccg gttgccgatg aattacggta tcacggctgg
    20041 aaagtgtgcg ttggtgcttc cggcaccgtg caggcgttac aggaaatcat gatggcacag
    20101 gggatggatg aacgcattac cctggaaaag ttgcagcaat tgaaacagcg agccattcat
    20161 tgcggtcggc tggaggaact ggagattgac gggctgacgc tggaacgtgc attagtgttc
    20221 ccgagtggtc tggcgatcct gatcgccatt tttaccgaac tgaatattca gtgtatgacc
    20281 ctggcgggcg gtgcgctgcg tgaaggcctg gtctacggca tgttgcatct ggccgtcgag
    20341 caggatattc gcagccgtac actgcgtaat attcaacgcc gctttatgat cgatattgat
    20401 caggcacagc gcgtagccaa agttgcggct aacttcttcg atcaggtgga aaacgaatgg
    20461 catcttgaag caataagccg cgatttgctc atcagcgcct gtcagcttca tgaaatcggc
    20521 ctgagcgttg acttcaaaca agctccgcaa cacgctgctt atctggtgcg taacctggat
    20581 cttcccggtt ttacccccgc acagaagaaa ctgctggcga cgctactgct caaccagact
    20641 aacccggtcg atctctcatc gctgcatcag caaaatgccg taccaccgcg cgtcgcagaa
    20701 caactctgcc gtttactccg cctggctatc atttttgcca gccgtcgccg tgacgatctc
    20761 gtgccagaga tgacattaca ggctaaccat gaactgttga ccttgacgct tccgcaaggt
    20821 tggctaaccc aacatccgct gggtaaagag attattgctc aggaaaacca gtggcagagc
    20881 tatgtccact ggccgctgga agtgcattaa tgagtaagtg ccggatgcga tgctgacgca
    20941 tcttctccgg ccttgattat ttccctcgtt ttgccgccat catcgctttc agattcgcca
    21001 gatggctttg ccctttctgc atccgttctt cggcactgac cactttgcgc tcctgttccc
    21061 aaatcagatc atcctgcggc agctccagca aaaagcggct cggctccggg cgcaccagtt
    21121 cgccgtactg acggcgttct ttacacagcg taaaggtcaa ttccttctgg gcgcgggtaa
    21181 tgccgacata ggccagccgc cgctcctcat cgatattatc ttcatcgata ctgctctggt
    21241 gcggcaaaaa cccttcttcc ataccgacca tgtagacata aggaaactcc agcccttttg
    21301 acgcgtggag agtcatcagt tgcacctgat ccagctcttc ttcactctca ccacgctcca
    21361 tcatgtcgcg taaagtaaag cgcgtcacca cctgggtgag cgtcatcggc tcatccagtt
    21421 cgctgccttc cagcatctcc gtcatccagc taaacagttg gttgacgttc ttcatgcgca
    21481 tttcggcagc tttcgtgctg ggcgatgttt cgtacagcca ggattcataa tccatgccgt
    21541 ggatcagatc gcgcaccgcg gcaatcggct cccgctccgc cagacgctgg atttctgcca
    21601 accagtgagt gaagcgggtc aatgcttcat aaccacgtcc gctaagcgtc tgactcaggc
    21661 ccatatcaaa gctggcggta aacatgcttt tattgcgcgt catcgcccac tcacccagct
    21721 ttttcagcgt cgccgggcca atctctcgct tcggcgtgtt aacgatacgc agaaatgcgc
    21781 tgtcatcgtc cgggttagtc agcacgcgca gataagccag caagtccttg atttcaggac
    21841 gagagaaaaa cgacgtacca ccagaaattt tgtacgggat gcggttttgc atcaggaatt
    21901 tttcaaacac ccgcgactga tagttaccgc gataaagaat agcgtaatct ttgtactgcg
    21961 ttttattgac gaagtgatgc gcaatcagtt caccagtgac gcgctcggct tcatgttctt
    22021 cgttattcgc gcttaaaact tttagctctg tgccgtaacc cagttcggag aacagacgct
    22081 tttcaaagac gtgcgggtta ttggcgatca ggatgttcgc cgctttcaaa atacgtccgg
    22141 aagagcggta gttctgctca agcttaatca ccttcagcgc cggaaaatcc tggctcagca
    22201 gcaccaggtt ttgcggacgt gcgccgcgcc aggagtagat cgactggtca tcgtcaccca
    22261 ccacggtaaa gcgcgcgcgg ctgcccacca gcagtttcac cagttcatac tggctggtgt
    22321 tggtgtcctg atattcgtcc accagcaggt agcgaatttt gttctgccag cgctcgcgga
    22381 cttcttcatt gcgttgcaga agtagcgtcg gcaataaaat cagatcatcg aagtcgagca
    22441 cattacaggc tttcaggtgt gcatcataca gcccataaca atgggcaaaa atacggtccc
    22501 gctcgccaat cgcactggct gccgcctggg acggtgtttt gagatcgttc ttccagttag
    22561 agatggtcga aatcaactgt tgcaggagaa ctttgtcatc ttcaatcagc ccttcggtca
    22621 actctttgag caacgccagc tgatcggtat cgtcaaacag cgaaaagttc gccttcatcc
    22681 ccagcgccgc atactcacgc ttgatgatat ccagccccag cgtgtggaag gttgagatca
    22741 tcagcccatg cgcctctttg cgacccagcg tctgtccgac acgctctttc atctcgcgcg
    22801 ccgccttatt ggtaaaggtc accgccgcga tatgtcgcgc ctgataaccg cacccgcgga
    22861 tcagatgggc gattttattg gtgataacac gagttttacc ggaacccgca cccgccagca
    22921 ccaggcaggg gccggtaacg aattcgacag cttgttgttg gccggggttt agacgcatag
    22981 gaaaatcgct caatgaaagt cgggagggga aaataacagc gtggtagtat agccagcctc
    23041 atccttacca ctcaaggcac gatcatggca aaaacagcag cagcactgca tatccttgta
    23101 aaagaagaga aactggctct ggatcttctc gtgcagatta agaacggtgc cgatttcggc
    23161 aagctggcga agaaacactc catttgccca tcaggcaaac gcggcggtga tttaggtgaa
    23221 ttccgccagg gtcagatggt tccggcgttc gataaagttg tgttctcttg tccggtactg
    23281 gagccaaccg gcccgctgca cacccagttc ggttaccaca tcattaaggt gctgtaccgt
    23341 aactaatagc aaggccttct ccgggagaag gccttgaact ctgaatcgct attaacccgc
    23401 aacagcaata cgtttcatat ccgtcatata gccgcgcagt ttcttaccta cttgctcaat
    23461 cgcatggctg cgaatcgctt catttacatc acgcagctgc gcgttatcta ccgcaccttc
    23521 cggaatagct ttacccaaat cgcccggttg cagttctgcc ataaacggtt tcagcagcgg
    23581 cacacaagcg taagagaaca gatagttacc gtactcggca gtatcagaga taaccacgtt
    23641 catttcatac agacgcttac gggcgatggt gttggcaatc agcggcagtt cgtgcagtga
    23701 ttcatagtaa gcagattctt cgatgatgcc ggaatcaacc atggtttcga acgccagttc
    23761 aacgcccgct ttcaccatcg cgatcatcag tacgccttta tcgaagtact cctgctcgcc
    23821 gattttgcct tcatactgcg gcgcggtttc gaaggcggtt ttgccggtct cttcacgcca
    23881 ggtcagcagt ttcttatcat cgttggccca gtccgccatc ataccggagg agaattcgcc
    23941 ggagatgatg tcgtccatat gtttctggaa cagcggtgcc atgatctctt tcagctgttc
    24001 agacaacgcg taagcacgca gtttcgccgg gttagagaga cggtccatca tcagtgtgat
    24061 accgccctgc ttcagcgctt cggtgatggt ttcccaaccg aactgaatta gtttttctgc
    24121 gtatgccgga tcggtgccct cttccaccag cttgtcgaag cacagcagcg agccagcctg
    24181 caacataccg cacaggatgg tttgctcacc catcaggtca gatttcactt ccgcaacgaa
    24241 ggacgattcc agcacgcccg cacggtgacc accggttgct gccgcccatg ctttagcgat
    24301 cgccatgcct tcgcctttcg gatcgttttc cgggtgaacg gcaatcagcg tcggtacgcc
    24361 gaatccacgt ttgtactctt cacgcacttc ggtgccaggg catttcggcg caaccatgac
    24421 tacggtgatg tctttacgga tctgctcgcc cacttcgacg atgttgaaac cgtgcgagta
    24481 acccagcgcc gcgccgtctt tcatcagtgg ctgtacggtg cgcactacat cagagtgctg
    24541 cttgtccggc gtcaggttaa ccaccagatc cgcctgtgga atcagttctt cgtaagtacc
    24601 cactttaaaa ccattttcgg tcgctttacg ccaggacgcg cgcttctcgg caatcgcttc
    24661 tttacgcaga gcgtaggaga tatcgagacc agaatcacgc atgttcagac cctggttcag
    24721 accttgtgcg ccacagccga cgatgactac ttttttaccc tgaaggtagc tcgcgccatc
    24781 ggcgaattca tcgcgcccca taaagcgaca tttgcccagc tgtgccagct gctgacgcag
    24841 attcagtgta ttgaagtagt tagccatggt gattcctcgt gatgttgtgc ttcttattgt
    24901 tcggttcgcg tttgcgagat tgaattcact atatgacagg aaatttattg cggaaattga
    24961 tatattcaca acgtcacatt gcaatttctg caacgtcaac attgagggct gcgccattgg
    25021 atttacgcga tctgaaaacc ttcctgcatc tggcggaaag ccgccatttt ggccgcagcg
    25081 cgcgggcgat gcacgttagc ccgtccacgc tctcacggca gattcagcgc ctggaagaag
    25141 atctcggcca gccgctgttt gtgcgcgata accgcacggt gacgctgact gaagcgggcg
    25201 aagaactgcg cgttttcgcc cagcaaacgc tgttgcagta tcagcagctg cgccacacca
    25261 tcgaccagca agggccgttg ctctatggcg aattacatat cttctgctcg gtgaccgccg
    25321 cttacagcca tctgccgccg attctggatc gcttccgcgc ggaacacccg tcggtggaga
    25381 tcaaactcac taccggtgat gcggcagatg caatggaaaa agtcgtcaca ggtgaagctg
    25441 atctggcgat tgccgggaag ccagagactc tgcccggcgc ggtggcgttt tcgatgttgg
    25501 agaatctggc agtggtgttg attgcacccg cattgccctg cccggtgcgt aatcaggttt
    25561 ctgtcgaaaa gccggactgg tcaacggtac cgtttatcat ggccgatcag gggccggtgc
    25621 gccgccgcat tgagttgtgg ttccgacgca ataaaatcag taacccgatg atttacgcca
    25681 cggttggcgg gcatgaagcg atggtatcga tggtggcgct cggctgtggc gtggcattgt
    25741 tgccggaagt ggtgctggaa aacagccccg aaccggtgcg taaccgcgtg atgattttag
    25801 agcgcagcga tgagaaaacg ccgtttgagc tgggcgtgtg tgcgcagaaa aagcggctac
    25861 atgagccgct aattgaggca ttctggaaga ttttgccgaa ccattaaccc gccaaaaaga
    25921 acctgaacgc cgggttattg gtttcgtcgt ggcaatcgta gcccagctca ttcagccggg
    25981 tttcgaaatc cggttcatgg tcgccaagtt cgaacgccgc cagtacgcgc ccgtagtcgg
    26041 tgccatggct gcgatagtgg aacaaagaaa tgttccagtg cgtacccagc gtgttgagga
    26101 agcgcagcag cgcgcccggt gattccggga attcgaagct gtagaggcgt tcctgcaacg
    26161 gatgtgacgg acgcccgccg accatatagc gcacatgcaa cttcgccatt tcgtcgtcgg
    26221 agagatccac cacgctgtag ccgccgtcgt tgagcatctg caaaatttct ttgcgctctt
    26281 cgaggccgcg gctcaggcgc acgccgacaa agatgcaggc gtttttggca tcggcaaaac
    26341 ggtagttgaa ctcggtgacc gatcgcccgc caagcagttg gcagaatttg aggaagctgc
    26401 ctttttcttc cggaatggtc accgccaaca acgcttcacg ctgttcgccc agttcgcagc
    26461 gttccgagac gtagcgcagg ccgtggaagt tcacgttggc accggaaaga atatgcgcca
    26521 gccgttcgcc gcgaatgttg tgctgggcga tatatttttt cattcccgcc agcgccagcg
    26581 cgccagaggg ttccgccacc gcacgcacat cttcgaataa atccttcatc gccgcacaga
    26641 tcgcatcgct atcgacggtg atgatgtcgt cgagatactc ctggcataaa cggaaggttt
    26701 cgtcaccgat gcgttttacc gcgacacctt cggcgaacag tcccacgcgt ggcagatcaa
    26761 ccggatgacc cgcatccagc gctgctttca ggcaggcgga gtcttccgct tctacggcga
    26821 tcactttgat ttgcggcatc agttgtttga tcagcaccgc tacgcccgcc gccagaccgc
    26881 cgccgccgac tggcacaaat acgcggtcga gatgggcgtc ctgctggagc agttccagcg
    26941 ccagcgtgcc ttgcccggca atcaccatcg gatggtcgaa cggcggcacc caggtgaacc
    27001 cctgctgctg tgacagttcg atcgctttag ctttcgcttc gtcaaagttc gcgccatgaa
    27061 gcagcacttc gccgccgaat ccacgtaccg catcgacttt gatgtcggcg gtggcggttg
    27121 gcataacgat cagggccttc acgcctaacc gcgcagaaga aaacgcgacg ccctgcgcgt
    27181 ggttacccgc agaagcagtg atcacgccgt gcgctttttg ttcttccgtc aggcccgcca
    27241 tcatggcgta tgcgccgcgc agcttaaagc tgtgcactgg ctggcgatct tcgcgcttca
    27301 ccagaatgac gttatcaaga cgcgacgaca gtttttccat tttttgtagc ggcgtaacct
    27361 gcgccgcctc gtaaaccggc gcgcgcagca ctgctcttaa atattcggca ccttccggag
    27421 caccggacag gggttgcgag tcagccatta ttaacccccc agtttcgatt tatcgcgcac
    27481 cgcacctttg tcggcgctgg ttgccaggct ggcgtaggcg cacagggcaa aggaaacctg
    27541 acgttcacgg tttttcggcg tccaggcttt gtcaccccga gcttcctgcg cttcacgacg
    27601 cgccgccagt tcggcatcgc ttacctgtaa ctgaatgcca cggttcggga tgtcgatagc
    27661 gatcaggtca ccatcttcaa tcagaccaat gctgccgccg cttgccgctt ccggtgagac
    27721 gtggccgatg gaaagaccag aggtgccgcc agagaaacga ccgtcggtga tcagcgcaca
    27781 ggctttaccg agacccattg atttcaggaa gctggttggg tagagcattt cctgcatgcc
    27841 agggccgcct ttcgggcctt catagcggat caccaccaca tcgcccgcga caactttgcc
    27901 accgaggatc gcttctaccg cgtcgtcctg gctttcgtac actttcgccg ggccggtgaa
    27961 tttgaggatg ctgtcatcga cgcccgcggt tttcacgatg cagccgtttt cagcaaaatt
    28021 accgtagagc accgccaggc cgccgtcttt gctgtaggcg tgttccagcg agcggataca
    28081 gccgtttgcg cgatcgtcat cgagagaatc ccaacggcaa tcctgcgaga atgcctgtgt
    28141 agtacgaatg cccgcagggc ctgcgcggaa catatttttt accgcgtcat cctgggtcag
    28201 cataacgtcg tattgttcca gagtttgcgg caacgtcagg ccaagtacgt ttttcacatc
    28261 acggttcagt aaccccgcgc gatccagttc accgagaata ccgataacac caccagcacg
    28321 gtgaacatct tccatatggt atttttgggt gctcggcgca actttgcata actgcgggac
    28381 tttacgcgaa agtttgtcga tatcactcat ggtgaagtcg atttccgctt cctgcgccgc
    28441 cgccagcagg tgaagtaccg tgttggtaga gccgcccatc gcgatatcca gcgtcatggc
    28501 attttcaaac gccgctttgc tagcaatatt gcgcggcagt gcactttcgt cgttttgctc
    28561 gtagtaacgt ttggtcaatt caacaatgcg tttaccggca ttgaggaaca actgcttacg
    28621 gtccgcgtgg gttgccagca gcgagccgtt gcccggctgc gacaggccca gcgcttcggt
    28681 caggcagttc attgagttag cggtaaacat cccggagcag gagccgcagg tcggacacgc
    28741 ggaacgttca acctgatcgc tctgggagtc agaaactttc gggtctgcgc cctggatcat
    28801 cgcatcaacc agatcgagtt tgattatctg atcggacagt ttggttttcc cggcctccat
    28861 cgggccgccg gaaacaaaga tcaccggaat attcaggcgc agggaagcca tcagcatccc
    28921 cggggtgatt ttgtcgcagt tagagatgca gaccatggcg tcggcgcagt gggcgttgac
    28981 catatactca acggaatcag cgatcagttc gcgagatgga agtgaataaa gcatcccccc
    29041 gtggcccatg gcaatcccat catccaccgc aatggtgttg aactctttgg caacgccgcc
    29101 agccgcttca atttgttcgg cgaccagttt accgagatcg cgcagatgga cgtgacccgg
    29161 tacaaattgg gtgaacgagt tcacaaccgc gataatcggc ttgccaaaat cggcgtcggt
    29221 cattccggtg gcgcgccaca gcgcacgagc acccgccata ttacgaccat gagtggtggt
    29281 ggcggaacgg tacttaggca tactttattt actcccagtg tctgtctcgt aaatgggacg
    29341 gtgcgtgccg tcccattttt tgtatttatt gattaacttg atctaaccag ccccatttat
    29401 cttcggtttc gccagtgaag aggccgaaga aggcttgctg aatgcgtttg gtaaccgggc
    29461 cacaacggcc ttcgccaatc tgaataccgt ctacgctgcg cactggcgtg atttctgccg
    29521 ccgtaccgga cataaagact tcatccgcca agtacaggga ttcgcgcgac agcacctgct
    29581 cacgcacttc aattcccagc tctttcgcca gtttgatgat ggcatcacgg gtaatacccg
    29641 gcagcgcgga ggaggtgaac ggtggggtga acagcacacc atctttcact tcaaacaggt
    29701 tttcgcctgc gccttcagag atataaccgt tcacatccag cgcgatacct tcctgataac
    29761 cgtggcggcg cgcttcgcta cccaccagca gggaagagag atagttacca ccggcttttg
    29821 ccgcagttgg gatggtgttt ggtgctgcgc ggttccagga ggaaaccatc gcatcgatcc
    29881 cctgctccag cgcttctgcg cccagatacg ctccccacgg gaaagcggcg ataatcacat
    29941 cggttgagta tcccgctggc gggtttactc ccatgccaac attaccgacg aagatcagcg
    30001 gacggatata ggcgctggtg agattgtttt tgcggatcac gtcacgacaa gcttccatca
    30061 gctcatcaat gctctgtgaa accgggaagc gatagatttt ggcggagtca tgcagacgct
    30121 gcatatgctc acgatggcgg aatacaaccg gtcctttgtg tgagtcgtag caacggatgc
    30181 cttcaaaaac cgaggtgcca tagtgcagcg cgtgcgacat cacatgcacc ttcgcgtctt
    30241 cccagcgaac catctcccca ttgaaccaaa tgtaatcagc tttcttcgtg gtcattttta
    30301 tattcctttt gcgctcaggc gcggatttgt tgtgatgtgg ttgtgctctg gcagatggca
    30361 acgtgtgcga cgtccaccag tttatttaac tgactaaaca gtaagtcgac cgaccgtggg
    30421 ctggcaacgg tcaattcgat atttatattt tgtgcatcgc tggcggcagc catattcatt
    30481 gagcagacgt ggaaaccacg atgacgcacc acgcgtaaaa cacgttctaa ggtttccgga
    30541 ttgaagcgag ccgatacatt gacctgatgt tgcatcatga taatttctcc aacatttctg
    30601 aattactggc acctggcggc accagcggcc agacgttctc aagttcgtcg attgagacat
    30661 gaagcaggta tggcccatca ctgttcagca tggtgtcgag tgccgcttca acctggtctt
    30721 tacgggtgat gtgttggcca gggatgccga aggcgctggc taacatgagg aaatcggggt
    30781 tatcggtaag ggtggtttcg ctgtatcgtt cctgaaaaaa cagttgctgc cattgtcgaa
    30841 ccatccctaa ccgttggtta tcgagtaaga cgattttcaa cggtaactgc ttgcgtttta
    30901 cggtgcccag ctcttgcaca ttcatcatga aagagccgtc accggagata cagacaacgg
    30961 tatcgttcgg tcgcgcgact tgtgcgccaa ctgccgccgg taaaccaaaa cccatggtac
    31021 ctaagccgct ggaggtgatg aaattttccg ggcgagtgtg ggcgatgtgc tgcgcagccc
    31081 acatctggtg ctgccccaca tctgtggtca cgacgcaatc cgcaggttta cgatccgaca
    31141 gttgttttaa caacaacgga gcgtagatag cgtcaccggg atggtcgtaa cgccaggaat
    31201 gttcatcacg cagctgcgcg cagtgttgct gccagtcatt gatatttaac ggctgctgta
    31261 atgctggtaa cagagcattt aaatcacctt gtaatgccac atgtgcctga cgcagcttgt
    31321 tcatttctgc cgggtcgata tccatatgga taacactggc gtgtggtgcg aaggtgttca
    31381 gtttgccggt cacccggtca tcaaaacgtg cacccacggc gatcagcagg tcacactcct
    31441 gcaccgcgaa gtttgccgct ttggttccgt gcatccccag catgcccaga tagtacggat
    31501 aatctgcttc tactgcgccc agccctttca gcgtacaggt ggcaggcatt tttgtggcag
    31561 cgagaaattc acgcaaagcc ggaactgcct gcgccatccc cacgccaccg ccaacgtaca
    31621 gcatcggttt ttgcgctttt gccagcatct ggcgcgcttg ctcgacttcg gcatgtggga
    31681 aagtcacttc gttttcaacg gtggtgaacc acggttccag atcgccgctg gctaactgga
    31741 tatcttttgg gatatcgacc agaaccggac caggacgacc tgagctggca acgtcgaatg
    31801 cttcagccat gatgcgcggc aactcttcca gagactgcac cagaaagctg tgcttggtac
    31861 aggctaacga caatcccagg acatccactt cctgaaatgc gtcggtgccg ataaacggtg
    31921 cggacacctg accagtgatg gcaacgacag ggatggaatc taacagtgcg tccgcaagcc
    31981 cggttatcag gttggttgcg cccggaccag acgtggcgat acatacgcca gttttaccgg
    32041 tagcacgggc ataaccgata gccgccattg ccgcaccctg ctcatgtcgg cacagcaagt
    32101 gctccacgcc gccgtcatac aatgcatcgt aaaccggcat aattgcgcca cccggataac
    32161 cgaaaacggt gttcacaccc tgtgcccgca acgcatgtac cacccactgt gcgccattca
    32221 tagttagttc cccgtcctga atcttgagaa acagaatttt gtgctgttat tcattgtctg
    32281 ctcctcggtt atgtttttaa ggtcaaaaaa acccccggac ctttcggtgc gggggtctta
    32341 gttcgttaag gcttgatctc taagcctttc ctcgtccaag tgcagccccg cacggtggga
    32401 taataatcac caccacgcta atcacgacca ggctaatcac tcgtagaagg gctgtcattt
    32461 tgtcttttct tgcatcttgt tcgaaggaat gcctaaagag ttaccataga ttttgcgaat
    32521 agcacaagat atttttgggc ctatttttat gatggcttta ataatttcaa tagaaattgt
    32581 tgttttttaa aaagaaaata gtgagtgaaa aaattcaatt tcagtataaa tgttgttccc
    32641 ttcctcgtag ttcatccttt gtccgctgca tttttgcgag catcattcca ccgccagaac
    32701 aactcaactt cacttgcaca aataaacagg aatcagccac taaaattcac gaattagcgg
    32761 gctacagcag cgatatctta tgcacataat ggtgactcaa ggagggttta tgtcactgtc
    32821 aattgttcat acccgcgcag ccctggaagt aaatgcgccc ccaatcactg ttgaggtaca
    32881 tatcagtaaa ggtctacccg gcttaacgat ggtgggctta acagaaacaa cggtgaaaga
    32941 agctcgcgat cgcgtgcgca gcgccattat caatagcgga tatgaatatc cggcgaaaaa
    33001 aatcaccatc aaccttgctc cagccgatct gccaaaagaa gggggacgat atgatttacc
    33061 tatcgccatt gcgttgctgg cggcctcaga acagcttaca gccaataagt tagatgaata
    33121 tgaattagtc ggagaactag cgcttacagg cgctctgcgt ggcgttcccg gtgcaatctc
    33181 cagtgcaact gaagctatta agtcgggcag aaaaattatc gtcgcgaaag ataacgaaga
    33241 tgaagtgggg ctaattaacg gtgaaggatg cctgatagcc gatcatctgc aggctgtctg
    33301 tgcgtttctg gaaggtaaga acactctcga acgcccgaaa ccaactgatg cagtatcccg
    33361 ggcgctacaa catgatctca gtgatgttgt cggtcaggag caaggaaaac gaggactgga
    33421 aattaccgcc gctggtgggc acaacctttt actgattggg ccgccgggaa caggtaaaac
    33481 aatgctcgcc agccgtatta atggcctgtt gccagattta agcaatgaag aggcactgga
    33541 gagcgctgcg atattaagtc tggtaaatgc tgaatcagta caaaaacaat ggcggcagcg
    33601 cccgttccgc tcacctcatc acagtgcgtc gttaactgcg atggtgggcg gtggtgcaat
    33661 tccagggcca ggtgaaattt cgttggcgca taacggcgtg ctttttcttg atgagctacc
    33721 tgaatttgaa cggcgtacac tggatgcctt gcgagagccg attgaatccg ggcagatcca
    33781 tctttcacgc acacgagcaa aaataaccta tccagcccgt ttccagcttg ttgcggcgat
    33841 gaatcccagc cctaccggac attatcaggg aaaccataac cgctgcacgc cagaacagac
    33901 attacgttat ctcaaccggc tctcggggcc ctttctcgac cgcttcgatc tctcactgga
    33961 gatcccatta ccaccccccg gcattttgag taaaacggta gtgccgggag aaagcagcgc
    34021 caccgttaaa caacgcgtaa tggccgccag agagcgccaa tttaagcggc agaataaact
    34081 gaatgcctgg ctggatagtc cggaaatacg ccaattctgc aaacttgaga gcgaagatgc
    34141 gatgtggttg gaagaaacgc tgatccatct ggggttatcg attcgtgcct ggcagcggtt
    34201 actgaaagtt gcacgaacca ttgctgatat tgatcagtct gacattatca cacgtcagca
    34261 tttgcaggag gcagttagct atcgtgcgat tgaccgtttg ctcatccatc tgcaaaaact
    34321 actgacataa aaaaagggca tttcgccctt tttattaatc gtcagaatcg gtgtagtctt
    34381 cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttaa
    34441 tacgcgtcat atacttggac cacacgcgtt ctgcttctgt cactggctca cgttcgccac
    34501 ggcatactgc tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca
    34561 actcattgaa ggcataacca tgacgctcaa gcagttgtgc ctctttgatg gtgaaatcac
    34621 catgacgaga gaatccacgt ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa
    34681 agctttccgc catcctgcac gctcctaatt ctttgaccga gctagttatg gcgcggagta
    34741 ttagttacgc ttgacagagt gtaaaacaaa acatttaaat cataacgaca aataattttg
    34801 cggagagcac tgtggatacg gaattgttaa aaactttcct ggaagttagc cgaacgcgtc
    34861 actttggtcg agcggctgaa tcgctctatc tgacccagtc agcagtgagc tttcgaatca
    34921 gacaactgga aaatcaactg ggtgtgaacc ttttcacccg ccacagaaac aatatccgtt
    34981 taaccgctgc cggtgaaaaa ctactgcctt atgcagaaac gctcatgagc acctggcagg
    35041 ccgcccgcaa ggaggtggcg catacctcac gacataacga gttttctatc ggtgccagcg
    35101 cctcgttgtg ggaatgtatg cttaatcagt ggttgggacg cttgtatcaa aatcaggatg
    35161 cccatacagg cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagc
    35221 tgcatgaacg ccagcttgat cttcttatta ccactgaagc gcccaaaatg gacgaattta
    35281 gtagtcagtt gctggggtat ttcactttag cgctttatac cagtgcccct tctaaactaa
    35341 agggagatct taactatctg cgactggagt gggggccaga ttttcaacag catgaggcag
    35401 gtttgatcgg tgctgacgaa gttcccattc tgacaaccag ttctgctgaa ctggcacagc
    35461 aacagattgc gatgcttaat ggttgcacct ggctacccgt cagctgggcg cgtaaaaaag
    35521 gcggcctgca taccgttgtc gatagcacaa cactttcacg gccgctttat gccatatggc
    35581 tgcaaaatag cgataaaaat gcgttgattc gcgatctttt gaaaattaac gtgctggatg
    35641 aagtgtatta atctgaatgg ctggcaagga tgccggtaga aggatttact tcggagagga
    35701 ttatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc aggggcggag
    35761 agactcgaac tcccaacacc cggttttgga gaccggtgct ctaccaattg aactacgccc
    35821 ctaattag
//
LOCUS       DAJVRL010000031        34995 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVRL010000031 DAJVRL010000000
VERSION     DAJVRL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.031"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..815)
                     /locus_tag="OQN02_001566"
     CDS             complement(<1..815)
                     /locus_tag="OQN02_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1642410.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OQN02_001567"
                     /pseudo
     CDS             907..993
                     /locus_tag="OQN02_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1398..1874)
                     /locus_tag="OQN02_001568"
     CDS             complement(1398..1874)
                     /locus_tag="OQN02_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1642411.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OQN02_001569"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OQN02_001569"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1642412.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="OQN02_001570"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="OQN02_001570"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1642413.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="OQN02_001571"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="OQN02_001571"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1642414.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="OQN02_001572"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="OQN02_001572"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1642415.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="OQN02_001573"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="OQN02_001573"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1642416.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="OQN02_001574"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="OQN02_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1642417.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OQN02_001575"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OQN02_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1642418.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="OQN02_001576"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="OQN02_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1642419.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="OQN02_001577"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="OQN02_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1642420.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="OQN02_001578"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="OQN02_001578"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1642421.1"
                     /translation="MSQLTHINAAGEAYMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="OQN02_001579"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="OQN02_001579"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1642422.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="OQN02_001580"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="OQN02_001580"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1642423.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="OQN02_001581"
     CDS             13849..14553
                     /locus_tag="OQN02_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1642424.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="OQN02_001582"
     CDS             14758..15471
                     /locus_tag="OQN02_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1642425.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="OQN02_001583"
     CDS             complement(15507..16463)
                     /locus_tag="OQN02_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCS1642426.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OQN02_001584"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OQN02_001584"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1642427.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="OQN02_001585"
     CDS             complement(17701..18462)
                     /locus_tag="OQN02_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1642428.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="OQN02_001586"
     CDS             18595..19005
                     /locus_tag="OQN02_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1642429.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="OQN02_001587"
     CDS             complement(18967..20073)
                     /locus_tag="OQN02_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1642430.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="OQN02_001588"
     CDS             complement(20084..21217)
                     /locus_tag="OQN02_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1642431.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="OQN02_001589"
     CDS             complement(21210..22946)
                     /locus_tag="OQN02_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1642432.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OQN02_001590"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OQN02_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1642433.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OQN02_001591"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OQN02_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1642434.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26024
                     /gene="rhlE"
                     /locus_tag="OQN02_001592"
                     /pseudo
     CDS             24837..26024
                     /gene="rhlE"
                     /locus_tag="OQN02_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OQN02_001593"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OQN02_001593"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1642435.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="OQN02_001594"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="OQN02_001594"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1642436.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="OQN02_001595"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="OQN02_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1642437.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="OQN02_001596"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="OQN02_001596"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1642438.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31428..31688)
                     /locus_tag="OQN02_001597"
     CDS             complement(31428..31688)
                     /locus_tag="OQN02_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1642439.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OQN02_001598"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OQN02_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1642440.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OQN02_001599"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OQN02_001599"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1642441.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34995)
                     /locus_tag="OQN02_001600"
                     /pseudo
     CDS             complement(33012..34995)
                     /locus_tag="OQN02_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8296 a         8972 c         9455 g         8272 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga acccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctagcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttgccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgtacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aacaggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgaccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaat gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 attatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgcggt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aaccatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgcgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgtccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacg
//
LOCUS       DAJVRL010000032        34937 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVRL010000032 DAJVRL010000000
VERSION     DAJVRL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.032"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..2261
                     /locus_tag="OQN02_001601"
     CDS             <1..2261
                     /locus_tag="OQN02_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS1642442.1"
                     /translation="AKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGIT
                     ENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGGN
                     FTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYL
                     NSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTT
                     SQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISA
                     SVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISAT
                     VSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAI
                     VMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYS
                     EQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSD
                     SYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQD
                     LQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNREL
                     MLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGS
                     EKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLR
                     GNVQTAQREVAP"
     gene            2258..3346
                     /locus_tag="OQN02_001602"
     CDS             2258..3346
                     /locus_tag="OQN02_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS1642443.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="OQN02_001603"
     CDS             complement(3434..4732)
                     /gene="exuT"
                     /locus_tag="OQN02_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCS1642444.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5216..6628
                     /gene="uxaC"
                     /locus_tag="OQN02_001604"
     CDS             5216..6628
                     /gene="uxaC"
                     /locus_tag="OQN02_001604"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1642445.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6643..8130
                     /gene="uxaA"
                     /locus_tag="OQN02_001605"
     CDS             6643..8130
                     /gene="uxaA"
                     /locus_tag="OQN02_001605"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS1642446.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8213..8764
                     /locus_tag="OQN02_001606"
     CDS             8213..8764
                     /locus_tag="OQN02_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1642447.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="OQN02_001607"
     CDS             complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="OQN02_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1642448.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10609..11574)
                     /gene="alx"
                     /locus_tag="OQN02_001608"
     CDS             complement(10609..11574)
                     /gene="alx"
                     /locus_tag="OQN02_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1642449.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11845..12843)
                     /locus_tag="OQN02_001609"
     CDS             complement(11845..12843)
                     /locus_tag="OQN02_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1642450.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12922..13614)
                     /locus_tag="OQN02_001610"
     CDS             complement(12922..13614)
                     /locus_tag="OQN02_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1642451.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13687..14190)
                     /locus_tag="OQN02_001611"
     CDS             complement(13687..14190)
                     /locus_tag="OQN02_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1642452.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14275..15411
                     /gene="rlmG"
                     /locus_tag="OQN02_001612"
     CDS             14275..15411
                     /gene="rlmG"
                     /locus_tag="OQN02_001612"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1642453.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15696..16010
                     /gene="higB"
                     /locus_tag="OQN02_001613"
     CDS             15696..16010
                     /gene="higB"
                     /locus_tag="OQN02_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1642454.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16007..16423
                     /gene="higA"
                     /locus_tag="OQN02_001614"
     CDS             16007..16423
                     /gene="higA"
                     /locus_tag="OQN02_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1642455.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="OQN02_001615"
     CDS             complement(16468..18486)
                     /gene="fadH"
                     /locus_tag="OQN02_001615"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1642456.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18712..21063)
                     /gene="ygjK"
                     /locus_tag="OQN02_001616"
     CDS             complement(18712..21063)
                     /gene="ygjK"
                     /locus_tag="OQN02_001616"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCS1642457.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21080..21499)
                     /locus_tag="OQN02_001617"
     CDS             complement(21080..21499)
                     /locus_tag="OQN02_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642458.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21477..21830)
                     /locus_tag="OQN02_001618"
     CDS             complement(21477..21830)
                     /locus_tag="OQN02_001618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642459.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21831..22151)
                     /locus_tag="OQN02_001619"
     CDS             complement(21831..22151)
                     /locus_tag="OQN02_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642460.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22285..23718)
                     /locus_tag="OQN02_001620"
     CDS             complement(22285..23718)
                     /locus_tag="OQN02_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1642461.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23781..24230)
                     /locus_tag="OQN02_001621"
     CDS             complement(23781..24230)
                     /locus_tag="OQN02_001621"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1642462.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24227..27304)
                     /gene="ebgA"
                     /locus_tag="OQN02_001622"
     CDS             complement(24227..27304)
                     /gene="ebgA"
                     /locus_tag="OQN02_001622"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCS1642463.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27487..28470)
                     /gene="ebgR"
                     /locus_tag="OQN02_001623"
     CDS             complement(27487..28470)
                     /gene="ebgR"
                     /locus_tag="OQN02_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1642464.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28688..29019
                     /locus_tag="OQN02_001624"
                     /pseudo
     CDS             28688..29019
                     /locus_tag="OQN02_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29061..30440)
                     /gene="ygjG"
                     /locus_tag="OQN02_001625"
     CDS             complement(29061..30440)
                     /gene="ygjG"
                     /locus_tag="OQN02_001625"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1642465.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30858..32378
                     /gene="aer"
                     /locus_tag="OQN02_001626"
                     /pseudo
     CDS             30858..32378
                     /gene="aer"
                     /locus_tag="OQN02_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32532..33155)
                     /gene="nfeR"
                     /locus_tag="OQN02_001627"
                     /gene_synonym="yqjI"
     CDS             complement(32532..33155)
                     /gene="nfeR"
                     /locus_tag="OQN02_001627"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1642466.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33432..34177
                     /gene="nfeF"
                     /locus_tag="OQN02_001628"
                     /pseudo
     CDS             33432..34177
                     /gene="nfeF"
                     /locus_tag="OQN02_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34231..34306)
                     /locus_tag="OQN02_001629"
     tRNA            complement(34231..34306)
                     /locus_tag="OQN02_001629"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34271..34273),aa:Ile,seq:cat)
     gene            34431..34937
                     /gene="mug"
                     /locus_tag="OQN02_001630"
                     /pseudo
     CDS             34431..34937
                     /gene="mug"
                     /locus_tag="OQN02_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8537 a         8974 c         9176 g         8250 t
ORIGIN      
        1 atgcgaagtc attatattta aatccagaca aaaatactgt taacgctttt atacatcagc
       61 aagacatcaa tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg
      121 cgctgggaat aacagaaaat agctatattg gtgcacactg gaatttcaac ggttatgatg
      181 cagatgatgt cagtgacagt aatactgacg tcagcgatct ctattatcgt tatgattttt
      241 tacgtcgtta ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag
      301 gcgggaactt tacctttaac tttctgccac tcggtgcaat cgatgggatg cgtatcgggt
      361 cgacactgag ctatttaaac caggcgcaaa gccagcaagg aaccccagta atggttctgc
      421 tttcgcgcaa ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc
      481 tcaatagtgg ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg
      541 cattaaaagt ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa
      601 ccggcggtct gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac
      661 aggtttctga tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc
      721 aatatgagct ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta
      781 ataactggac ggcagattta ggcagggtgg ggaatcttgc aatcagcgcc agcgtgttcc
      841 gtaacgatga cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc
      901 cgacgttggg cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag
      961 agagctataa cgccttaagc tgttatgaaa gtatttccgc gacagtttca ctgaattttg
     1021 tcggctggaa tatgatgctg ggttatacct gcacacaaaa taacactgat gatagtttgc
     1081 gttgggataa acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta
     1141 tctctgaaac tgtacaactt agcgcttccc gcgctattgt gatgcgtgac tggattttga
     1201 gtacgtcagt cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct
     1261 tgtacttatc gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt
     1321 caaccaatgt gcctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc
     1381 agttatcgca taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg
     1441 gaggcctgaa caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg
     1501 gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg
     1561 cctttacggg cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag
     1621 ccagtggtac agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac
     1681 aggatgaaga gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc
     1741 agcttggtca aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg
     1801 aggttaacga cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag
     1861 ggaatcgtga attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct
     1921 ttaactacaa ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg
     1981 tggggctgaa cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta
     2041 acggcagcga aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga
     2101 gtgttgtaaa gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga
     2161 cttattcaca attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac
     2221 gtggaaacgt tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc
     2281 gatattgggc ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga
     2341 aattacgtat gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc
     2401 ggcaggaaat aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg
     2461 tacagctaat ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat
     2521 gcgttggccg gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc
     2581 gctaggatgc aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
     2641 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
     2701 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
     2761 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
     2821 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga
     2881 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
     2941 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
     3001 tgaagggcga ccagctgcag ggcgcagaga cggttacttc tctatctatc gtcagggagg
     3061 gaccaccacc gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac
     3121 cggtgggcaa attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa
     3181 acgtgtgcgt ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc
     3241 cttgactctg gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg
     3301 caaattgtca gtaatcttta cgccttcctt gccaaccatc aattaaccct gcaaaccgga
     3361 caaacgcttt tttctatata aacgacgaag ccgcctgata aggcggcttc gtcgttataa
     3421 cttacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     3481 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     3541 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     3601 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     3661 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     3721 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     3781 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     3841 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     3901 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     3961 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcaa
     4021 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4081 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4141 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4201 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4261 ttgcccatac caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt
     4321 agcctactgc gatggaacgc tctttcgccg ggaaccattc ggagctggct ttcagacccg
     4381 ccgggatcat cgctgcttcc gcggcaccga ccgcaccacg agcaacagct aggccacccc
     4441 agctacctgc cagcgcggtt gcaccacaga acacggccca cagcacagca aacattgcat
     4501 aaccgatttt cgtacccagc acatccagta cataacctgc taccggttgc atgaccgtat
     4561 aagcagcaga ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt
     4621 ccatcagagt tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc
     4681 cgagcgtcac cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc
     4741 tcatcgtttt gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt
     4801 tagttacgac cgggtaaccg aaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt
     4861 tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg
     4921 caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaacc
     4981 ttaaaaggtg agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag
     5041 cgaggccagt actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa
     5101 ctacgtttat tgatctaact caagaaaata tcttcggact ctggaaattg gtgtgataac
     5161 tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac
     5221 tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga
     5281 ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc
     5341 ggaagactat cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg
     5401 gcgcgctatg cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg
     5461 tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca
     5521 ctggacgcac ctcgaactgc gtcgtccgtt cggtatcact ggcaaattgc tttctccgtc
     5581 aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc
     5641 gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga
     5701 ttctctggag catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc
     5761 gagctggcgt ccggacaaag ccttcaatat cgaacaggcg acctttaacg actacatggc
     5821 gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt
     5881 gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga
     5941 cgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg cgcgccgtct
     6001 ggctggcgaa accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt
     6061 ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg
     6121 taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa
     6181 cgaccgtccg atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct
     6241 gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat
     6301 gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg
     6361 gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct
     6421 gctgagccgc tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca
     6481 cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc
     6541 accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg
     6601 tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc
     6661 catgcgctgg ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt
     6721 gtcgataacc agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta
     6781 acggatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg
     6841 gcggatattg cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat
     6901 ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcct
     6961 gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc
     7021 ctgccaaccg tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa
     7081 gagaccaaca acgccgaagg taccgacggc gtgttcctct tcagccacac ctatggctgc
     7141 tcacaactgg gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac
     7201 ccgaacgcgg gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca
     7261 ttccgtgaaa cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag
     7321 caggatgatg agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc
     7381 aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt
     7441 tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg
     7501 attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag
     7561 ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac
     7621 gacttcaaac agtactttat tgcccatgat cagccgatct acgaaaaccc atcaccgggg
     7681 aacaaagcgg gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg
     7741 ggttccagtg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg
     7801 aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc
     7861 tgccatatgg tgctgttcag tactggtcgc ggcacgccat acggtggatt tgtgccgacg
     7921 gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac
     7981 gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc
     8041 atcgttgagt ttgccaacgg taagcaaacc tgcaacgagc gtaacgactt ccgcgaactg
     8101 gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc
     8161 gtttcataat gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc
     8221 gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt
     8281 caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt
     8341 acattttttt cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg
     8401 tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac
     8461 tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac
     8521 gattgtcagt acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg
     8581 gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat
     8641 gattgagggt agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca
     8701 gaaaagggga attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg
     8761 ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct
     8821 gccgcagtga acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat
     8881 acgccgataa taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac
     8941 atattacagg ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag
     9001 gcacacagag aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc
     9061 agcgtattaa ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg
     9121 atggtggctc ccagcggaat agaaactgaa taggtatcgc gatccagatt cagcttttca
     9181 cacagcgcca tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca
     9241 ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac
     9301 accagcaatg ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc
     9361 agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc
     9421 ccaaaaatac cgaacggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc
     9481 gacatatcat tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg
     9541 ccgatcgccc acaccagaat cccgatgtag ttacctttca gcagggcgtc gatggggttg
     9601 gaaaccatgc tcattaccag tccgcgcatc acttcgacaa tgcctgacgg cggcgaaata
     9661 tcacccgcgc tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg
     9721 gccagagctg cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt
     9781 ttctgcccgt gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg
     9841 gcaacagctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg
     9901 ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc
     9961 aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga
    10021 tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt
    10081 aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc
    10141 tggattttaa agagaggtca tgttttgcaa cttaatggtt gcatttattt aacataaaca
    10201 gagggaagga ctggctacta aggctgatta gataacatat gggcaaaact atttggaata
    10261 tgagaacggt tgcgctcctt taacgtgtta tgccatataa agtcactggc gcagggagcg
    10321 cgcagggggc ggccaatcgc cgccgccccc tgcaccccca ggctctggcg aacaaaatcg
    10381 ccgctgcgcg gttccctcag cctatccctt gcggctaccg ggtcgggcgc aaggtaacgt
    10441 ccctgtaaga tgcgccctcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac
    10501 caacacccgt gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac
    10561 ctttgtctgg aatgagttat ctctaggaat ggcttaatat acagcgaatc attctccccg
    10621 ctgcttatca tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc
    10681 aaacaccacg cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt
    10741 gataccgata aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc
    10801 tacgcccgcc agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt
    10861 cagcacaatg aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa
    10921 aatcacgtcg ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa
    10981 tccattctta cgcacaaaga aatgctcgtt gtcgatggtg tcggtcatgc gcaaatgacc
    11041 gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc
    11101 catcttcacg ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg
    11161 cgaaatcagc cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac
    11221 gccatacacc agcacgcgac gttgtaatgc cgccggaacg gagaaatagc tgaacaacat
    11281 cagccagaca aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa
    11341 ggccagggcc tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa
    11401 agcggcgtta aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt
    11461 catggtcatg gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat
    11521 ggcgacaaca acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt
    11581 tccttacaca taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    11641 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    11701 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    11761 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    11821 ctgttacata ccttttacac tgttttactt tggcaagtat agttttacgc tatctgccgg
    11881 aaaaatcacc ccagtttggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac
    11941 cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    12001 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg
    12061 cacaaagcag actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    12121 ctgaatctcg cttcccagga cagaatcact gacctttgag tgctgcaagg tgacgctgaa
    12181 atcaccgtaa tccatcacca ccacgccgtg ggcgtcaacg ccgcttgcca gcaaactcgc
    12241 cgttgcctgc acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa
    12301 gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc
    12361 gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag
    12421 tttgcctgct ttcggcagcg cctggcgcaa caaatgaaag ttcggcaggc aggcggtttt
    12481 aaatgcctca aacagcacca cctgattttc gcgcgcacag gcaatggcgg catccacttc
    12541 cgccagattc gacgccagcg gtttttcgca aatcacatga attttatggc taaggaaaag
    12601 ttgtgtctgg gaaaagtgca gggaattcgg gctggcaata tacaccgcgt caatggcatc
    12661 gctttccgcc atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcgaa
    12721 gtgctgagcc tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc
    12781 atgggcggcc tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat
    12841 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    12901 ttcgacgtgc gcagatacgc attattcttt cgccggacag ccatgctcac taaacggccc
    12961 aatcatgacc gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac
    13021 cgcgctgaca cgagcgagtt tctcccgcag ttgagtataa aaaccgtgct tcatattgag
    13081 gtctggcgcg ttaaacccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg
    13141 ttggttatga aattcttgcg agataatggt gatatggttt tccccaaaca cctttttggc
    13201 acgcactacc gaatccagcg ttgagaatcc ggcatagtcg caaaagatca ctttcgccgg
    13261 tacgccttta gcgatcaaag cctgctgcat acctgatgct tcatcgtaat ttttacgtcc
    13321 gttatcgcca ctcaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt
    13381 atcaatacgt cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt
    13441 acgcgccggg acggcgttga catcgcccca ggtcagctgc ttgctggcgt tgaccataac
    13501 gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag
    13561 agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt
    13621 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaaagata acgcctgaca
    13681 tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc gggtgtcgaa
    13741 ctcaagctgg tggtactgcg gctccatatg gcagcacaat tgatagaacg ctttgttatg
    13801 ctctttctcc ttcaggtgcg ccagctcgtg caccacgatc atgcgcaaaa acggttccgg
    13861 cgcgttgcga aacacggtag cgacgcggat ctctgctttt gctttcagct tgccgccctg
    13921 cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga tcttattgtc
    13981 atacatcact ttattgatcg gcggggcatt acgcagaaac tgatttttca gatcctgagt
    14041 atattgccag agagctttat cggtggcgta gtcgtgtgtt cccggatagc gttttgccag
    14101 cacatcgccc agacgctgtt cgttaatcag tgttcgcacc tgcgaaagca gctgctcggg
    14161 ataaccctga agataagtaa gattgctcat aaccgcccac gaaaaagaga aaaagggtat
    14221 actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg gccgatgagc
    14281 cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt
    14341 aacccgctac aggcgtggga agcggcagat gaatatttgc tgcaacagtt ggacgacaca
    14401 gaaatccccg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg
    14461 gcggaacata agccgtacag cattggcgac tcatacatca gtgaactggc gacgcgcgag
    14521 aatttacgcc taaacgggat tgatgagtcg agcgtgaagt ttctcgacag caccgccgac
    14581 tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc attgctggaa
    14641 cagcaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat tgccggtgcc
    14701 aaagcccgtg acattcacac ttccacgctg gaactgttcg aaaaagtgct cggcccgacc
    14761 accaccacgc tggcatggaa aaaagcgcgc ctgattaatt gcacgttcaa tgaaccgccg
    14821 ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg gactatccat
    14881 aaccatgcga atgtcttctc ccgcaccggg cttgatatcg gcgcgcgctt ctttatgcaa
    14941 catctgccag agaatctcga aggggagatt gtcgatctcg gttgcggtaa tggcgttatt
    15001 ggtctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg
    15061 atggcggttg cttccagccg tttgaacgtt gaaaccaaca tgccagaggc gttggatcgc
    15121 agcgagttta tgatcaacaa cgcgctctcc ggcgtggagc ctttccgctt taatgctgtg
    15181 ctctgcaacc ctccgtttca ccaacaacat gcgctgaccg ataacgtcgc ctgggagatg
    15241 ttccactacg cccgccgctg cctgaaaatc aacggcgagc tgtatatcgt tgccaaccgc
    15301 catctggatt acttccataa actgaagaaa attttcggca actgcaccac tattgcgacg
    15361 aataataaat ttgtggtgct gaaagcggtg aagctgggac gtcgtcggta agattgggag
    15421 ttacctgccg gatgcggcgt aaacgcctta tccggcctac gttcagcacg ttacccctag
    15481 gccggataag acgcggcaag cgtcgcatcc ggcaatgtct gctcagttct ggaatcgcgc
    15541 ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta gcgtttttct
    15601 acgtttattc ttccgtcaca cagataaatt catccgttgc caatctgtca acggatgtta
    15661 tcatattgcc aatttgtcaa cggacgtgat gaagaatgca cctgataact caaaaagcat
    15721 tgaaagatgc tgcggaaaaa tacccgcaac ataaaacgga gttggtggct ctggggaaca
    15781 cgattgctaa gggatatttc aaaaaacctg agtcattaaa agcagtattc ccatctctgg
    15841 ataacttcaa atatctggat aagcattatg ttttcaatgt tgggggcaat gaattacgtg
    15901 ttgtagcaat ggtctttttt gaatcgcaaa agtgctacat acgtgaagtt atgacgcata
    15961 aagaatacga tttctttacc gctgttcatc gtactaaggg gaaaaaatga ttgcgattgc
    16021 cgacatcttg caagcaggag aaaagctaac tgctgtggca ccttttctgg cgggtattca
    16081 gaacgaggaa caatacaccc aggcgctgga actggtagat catctgctgc tcaacgatcc
    16141 tgaaaacccc ttgctggatc tggtgtgtgc caaaataacc gcgtgggaag aatcagcgcc
    16201 cgaatttgcg gaatttaatg ccatggctca agccatgcct ggcggtatag ccgtgattcg
    16261 tacccttatg gatcaatatg gtttaaccct ttccgatctg ccggaaattg gcagtaaatc
    16321 tatggtgtca cgcgttttga gcgggaagag gaaattaacg ctggaacacg ctaaaaaatt
    16381 ggcaacgcga ttcggcattt ctcccgcctt gtttattgat taacgcgacg tgccggatgc
    16441 gttgcatccg gcgcttcaaa taacgattta aatctccagc gccagccgtg ttccctgggc
    16501 aattgcccgt cgtgcgtcca gctccatagc cacatcgcag ccgccaatta aatgcacagt
    16561 tttcccgcta tcaatcagcg gttgcgccag cgcgcggttt ggctcttgcc ctgcgcagat
    16621 caccacattg tccactgcta atacctgcgt ttcgccgttg atcaccacat gcagcccgtc
    16681 atcgtcaatc ttctgataac ttacgcctgg gatcattttc acaccccgcg agagcaggat
    16741 ggtgcgatgg atccagccgg tggttttgcc taacccctgt cccggtttgc tggctttgcg
    16801 ctggagcatc acaatttgcc gtgggctacg cgggatctgc attccctgcg ggcttaagcc
    16861 accagcctgt tgtaggctgc tgtcgatccc ccactcatta cagaacctgg cgatattctg
    16921 gctggtggat tcgcccggct gacttaaata catcgccgta tcaaaaccaa tcccgccaca
    16981 accgatgatg gcaaccttgt tgcctaccgg cgctttgtcg cgcagtacat cgagataact
    17041 caataccttc ggatgatcga tcccgtcgat aggcggaaca cgcggcacga tcccactggc
    17101 gaggatcgtt tcatcgaacg cctgtaactg atccgccgtc acggtgtgat tgagtttcag
    17161 cgtcacgccc gtcacttcga tcatccggcg gtagtagcgc agcgtttcgt aaaactcctc
    17221 tttgccgggg atctgtttgg cgatattaaa ctgcccgccg atctcgctgt gcgcatcaaa
    17281 caatgttacc tgatgcccac gcgccgccgc gttaatggca aacgccagcc cagcaggtcc
    17341 agcgccgacc accgccagat tttttttctg cacggcggga aggattggca ttttggtttc
    17401 gtggcaggcg cgaggattca ccaggcacga ggtgactttg ccaacgaaga tctgatcgag
    17461 gcagccctgg ttgcagccaa tacaagtgtt gatctcatcg gctcgccctg attgcgcttt
    17521 tgacagcagt tccgcatccg cgagaaacgg tcgcgccatc gatactatat cggcatcgcc
    17581 gcgcgagaga atatcgtcgg caacctgcgg atcgttaatc cggttggtgg ttaccagcgg
    17641 cagcgagacg tggcctttca gtttgcgcgt gacccagcta aatgcgccgc gcggcacggg
    17701 cgtggcgatg gtcggaatcc gcgcttcgtg ccagcctatt ccggtgttga taatggtcgc
    17761 gcccgccgct tcaatggcct gcgccagttc taccgtttcg gcaaaagtgc cgccgccttc
    17821 gaccaggtcg agcatcgaca accggtaaat aataatgaag tcgttgccga cgcgttcgcg
    17881 caccgtacgc actacttcta cggcaaatcg catccggttg cggtaatcgc cgccccactg
    17941 gtcactacgc tgattggtgc gcagcgtcag aaattcgttg atcaaatacc cttcagagcc
    18001 catcacttcg acgccatcgt atccggcctc ccgcgccagt tgcgcgcagc gggcgaaatc
    18061 gtcgatcagt tgcaggatct cttcatgggt gagttcgtga ggaacgaaac ggttgatggg
    18121 ggcctgcaat gcggacggtg cgaccagatg cggttggtag ctgtagcgcc cggtatgcaa
    18181 aatttgcagg gcgattttgc cgccttcctg atgtacggct tcggtaatgg tgcgatggtg
    18241 tgggatctgg ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc ctgttaaatc
    18301 tggtgcaatg ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa
    18361 cgcagccagc cgttcagcac cgtccgggta ttcctccagc ccggtgtgca ttgaacccat
    18421 caacacgcgg ttttttaacg tggtaaaacc taaatccagc ggggcgaaca gcgacgggta
    18481 gctcataaaa ttgtccagta tgtaaaataa ttgttatgta gtcggatgag ttctaattta
    18541 gccgtcgatg gagaaaaggg aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa
    18601 tacgcccggt ggtaacgccg gatgcgacgc ttgccgtgtt ttatccggct tgggggattg
    18661 cacatgtagg gcggataagg cgttcacgcc gcatccgcca gccgttgcaa attactgctt
    18721 acggaaaaaa tcgttataca acatatacaa atgagcggca ctccaggaga aatttggtgc
    18781 gccttgctgc gcgcctgtca gcgggttgta attctcctga attgggccat cggcggttaa
    18841 tcctttggcg tgcctgaaga acgtatccgc cagcttcagg gcatcatcac gataaccgta
    18901 acgctccatc cctttcagac caaaccagaa ctgatccacc catacgcgcc cgcgccagta
    18961 gatatcagcg ccaaaagccg gattggttaa cgccgccgtt cccagcggaa caaaggtgtt
    19021 gaactcttta ggatcgagca tcaccttcac caccgcgtcg gcattggcct gcgttgccgc
    19081 accgttaaac agcggcgacc agccttccgg ccctttaccg cgctcaacaa tcggtttgcc
    19141 cgcgcagccg ttcgccagtg gtttatcttc aatacgtacg tcatagtaga actgggtagt
    19201 cgggtcgaac atacaggtgt tgatgtagtc cgcgagctgc tgtgccaact ggcgatagcg
    19261 tttggcctct tctggcttac cgaggatggt tgccatctcc gccagataat ggttatcgct
    19321 gtacatatag ctggcctgat ccaccgactc ctgcaatagc gagtagccca gcaacgttcc
    19381 gtcctgactg cggttttcgg cgaatttcac cgtccagtcg ctacgtttgc cgccattagc
    19441 gacatattta tccaactgtt ctttgtcgat aaaaccaaag acggcggcat catcacggcc
    19501 cgattcccac gacgcagcga cctgtgccgg aatttccaga ctgtcgtact ggcctttctc
    19561 caccacgcgg gcgtagttgt tcagcccaga ctgcgtctct tctttatcgc cttttttcac
    19621 cgtaaacagc atctcgccgc tctcggtgtt gtgggctttg tcgcgggtcg cgccatattc
    19681 cggcaccccg ttgccgttat gatcgcggtt acgtaaccac cagtcgtgat aggccaccag
    19741 tttcgggtac atctctgcca accaggcttt atcctgggtg acgttgtata cttccatcac
    19801 cgaccaggcg gcaaggctgg gtttggtatt acgttcgttc cagttgccgc catcgccacc
    19861 acgctcgggg ctaaggttcc acgctatcag gtcggggaca aagcccacat cctgcggacg
    19921 cacgctgtcg ccaggctgga tctgccagga gaagaccgcg cggatattct ctttggcgat
    19981 atccggattg aaatgcgcca tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg
    20041 attgccggag aaccagcgtc cggtcaccga cggcgtgacg gtgttataat tcaccgcacc
    20101 gcccggtgag cgccagttac cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg
    20161 ttccggcgtc gcatccggat tggttaaccc tttcttcagg tattcttccc agcgttgctg
    20221 cgaggcggtg agataaaacg ccggacgcgc cagaatatcg cggatctgca tttgctcttt
    20281 gctaacttcc tgagcggtca gcagatggga ataggtggtg tagagcgtgg tcgaaccgtt
    20341 gatatgcgcc ttactggtaa agcgattacc gttgatttca gtctgcaccg gcagggattt
    20401 atgcacctga tattccgatt cgccggaggt cagcagatcc caggtggcgc gcactttgcc
    20461 aaaggtgact ttcaggccat cacgggtggc gctgattttg cgctgatagt cagggtattc
    20521 gccagcaatg gttttatcgg aaagcggttt cccttctttc gcttccagtt tttccagcag
    20581 ttcgccatcc cacaccagat ccagcggttt attgctgatg attttagttt ccagtagtga
    20641 cgtgcgcggc gtggcgaagc gcagagtcat ttcgacctgc acatcttttg ctgtcagttt
    20701 ttgcaccagc gcgccgggaa tactgtatgc ctccagcgtg aagttgactt tcttgccgtc
    20761 ttgccagaca gtcaggcggt cgaaattgct ggccataaag ttgatgtatt cttccgtcag
    20821 cagcgcaacg cccggaaagc cgcccatggt gttcgggccg tctggcaaca gatgaccatg
    20881 ccaggctccg agatcgaaaa acggattaaa acgctggtga tcgtcgtaat cgtaatcctt
    20941 catgtactgc ggcgcaccag tacggttaat cacgttttta taattgtcgg cgttagcggc
    21001 atgagcggaa aaacttatca gcagagcgca ggttactggc gttaaaatag ttttgatttt
    21061 catgagatta cagtccttat taccagtaga aatattgcat caggaagatt tggtcgctgg
    21121 gatcgttaga ggcatttttc atgtagaagc ggctatcaaa agccgtggcg aaacgtccgc
    21181 cgccatattc gtaccagata ccaaactgca tgaaagaggt ggtttcatcg tcagcgtggt
    21241 cagggcgatg tttggcgtag gtgtaagcgg tggagagata aacgcctttt aacgcttcat
    21301 acatggcgct caccatcatc ccgctttcac ttcccggtac gtccagcccg tcggcacgtc
    21361 cggtatggtg ccaggcgcga gcggcaaagt taggggtaaa caggcccgtg agatagagtt
    21421 gtcctgtgcc gtcggtaatt tccagaccat tcatccaggt gatgccttcg gcaaggtcat
    21481 actggatgta accgttcatc cattgccgga taggtgtatt tgtcgtcgta gcgatcgtac
    21541 ttgccaaaat gtagccaggc tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg
    21601 ctataacgaa agttgccgct taagttctgt accttcagcg cggtcataat gtcgcgggta
    21661 ttgggaatga cgtagcccaa atcgggtgtg aagtcgcccc accattgcag gtcatccagc
    21721 gaggaatcct gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca
    21781 tagaaacggt taatcccacc ttcgaaacca ccccagctat ggtcaggcac ctaggcatta
    21841 ccgtcgtggt cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg
    21901 tacttcatct ccaggccacc ttcaatatag gtgccgtcgc tgtatttatg tttattctca
    21961 ccctctaaat ccatatatcc accaagccct aattcgccgt aaaaacgaaa tgaaggagaa
    22021 gatgtcgagg taattgcggg tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct
    22081 cctgccgcag aaaaggcgta gcaaaacggc agagcaagta atgctctgca tggtgcagtg
    22141 attaatttca ttgtgttaat ccatgagcta aagaaaaagt acagcctttt atcgttgtaa
    22201 ttataaaaat ataaaggcag aaactatttt ccctctcctc atccaggaag agggaaaatg
    22261 cctgctcatt ttttattagt ctgcttaatc aacattgctg gctggcgtcg cttcaatatg
    22321 gcgctcttca gccgttaatc cctttatata tttgctgtat ttccaccacg caaagccgag
    22381 gaagataaca ataccgccga cgttataaaa aatgatggtc agaatattcg cgccagtcgg
    22441 gaatgtcgaa gcgacaaacc ctacggcaaa tatcgcaatc agcattgaaa caacgataat
    22501 accggtgcgg cgggagccca tacggaaatc gcgtggcagg tgatctaatt tggcgcgtaa
    22561 attcaggtaa gccagcatga tgaataacgg cggaagcatg gacgctgcgg cggtcatatt
    22621 aataatggta ttcatcagat cctgcacggt attggaaccg agcatcggga taatcatcag
    22681 cgggatgacg atcagaaact ggatccatgc tgcgcgcgcc ggaacgccgt tttcgttaag
    22741 ttcgacggtt ttcttaccaa agatgccttc cgggatttcg gagaagaaaa ttttcaccgg
    22801 cgttgcggtc cacatcagca gggaaccgaa cattgcggta aaggacacca gaccgacaaa
    22861 gcgattcatc aacgcttccg gcagaccaaa atacgccgcc atgccgtgga atacctgcac
    22921 cgatccgccg gtaaatttca actctttgct gctgacgaag acgttaatca gcaccgagga
    22981 gacggaatac agtacgccga taaaaatccc ggcgaggatg atcactttaa cgaacgattt
    23041 cgaaccgcct ttgacgtcgt taacgtacac cgcgacggac tccgcgccac ctgcggccat
    23101 aaagatccag gtggtaacgc cgaggaacgc ccagttgaag ttcgggatca tcgcgtcaac
    23161 ggtgatcgga tcggcaggct gtacgccgcc aaccagtgcc gtacctgcca gtaaaatgta
    23221 ggagagcgtt aacagcagca tcagcgttga agtgacggag gtaattggcc ccagcatttt
    23281 cgccccgttg gtggaaaccc aggtggagaa ggcgaacagc accatactga taacggtggt
    23341 ggcaaccggc gtcataatat attcgtagcc gaggaaggca tacgaagcat aagcaataac
    23401 gcgcggcaac agcgaggtga aaaagaacag atttacgaac cagtaagtgt aggcagtaat
    23461 aaatgcccaa cgtccgccca gcgaactttt tacccacgcg tagacaccgg cttctgagtt
    23521 tttatttaac gaaacaaatt ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt
    23581 cgcgaggaaa aacatcggtg ccgaggccag tccaagctca atattattat tgataacgtt
    23641 gttaaagctg taaacggcgg caaaagtcag cgagagcaag ccgaatttgc cgattgtatt
    23701 acgtttggta tcagacataa cacatctccg aaaatgtgta gggtagcggt ggcggcaaac
    23761 tccttttgcc gccgtagttg ttatttgtta tggaaataac catcttcgat ggtgactttg
    23821 agaaccactt ttttgaccgc gttattgcag ataaaccgat acgcttcatg gatatcgcaa
    23881 ataacgattt gcccttcgtg gacctctacg gtttctccgc agccttttaa atattcacgg
    23941 tcagtttcat cacgataata ttccactacc tgtaatgtct ctttcggcgc atattcaatt
    24001 ttttgctgcc cttgcaggta gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc
    24061 gcatcggtcg cggagtcatt ttccacacgg taggtcaatg aatcaccgat ggagtgggcg
    24121 acgccaggct gaatgttgtc gatattttca atcgcttcaa cgcagcgttg ccacttcttg
    24181 ccagaggcgt aaatctggcg gaactgttct aagttatcga tgatcctcat tgcttattct
    24241 cgctgtgcaa attcgtggaa aagaaccctg cgccgaactc atacgacgcc aggctttgcg
    24301 cggtagcttc tccgccagaa accggcagca acgtaaagcc gtagctgaag tcacggaacc
    24361 agacgcgcca ggagtccagc acctcgctgc cccaggagtt ggagccgagg ccaagtagct
    24421 ggtggtcgag gttcagggtg atgtcatcac tgcgctgcag ctcgttacag tgctgggcag
    24481 cgtggatgtt ttcctgggta tagtgccagg cgctgaagtt aattgggcgc tgcggaacca
    24541 ccagcagacc gttgccgtgg cggttagtca gtgccgtcca gcggacatgc tgacggttgc
    24601 cgttgttctg cgggaaggga tagttctcga acatggcatc gacggtgctg cgccagatat
    24661 cgatgatgtt agcctgctgg ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg
    24721 ccacctgatc gtattcgccg ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg
    24781 gatagtcgcc gtaacgctcg ccggaaagcg ccacgttaac ctggccatcg gcagcgatgc
    24841 gccagatgta ggtacagcgc atcccgaagt caaacaccgg cggtgcaata actgtgcggc
    24901 tgatgatcaa cacttcacca tcgctctgtt ctacggcaaa gtcgcgcaga tgttcctgca
    24961 tgatctgcaa atgattcggc tgccacaacc cttcgtactc ctgcttgtgg ttgtcgatca
    25021 tcggcttgaa gaagttgatc tttggctcgc gggtcagcag cgactcgcca tttacctggc
    25081 aggatgtcgg tttgccactc atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg
    25141 tgcagctcaa acgatcgtct tccagcgtca gcggacgcgc attatttggt gcgaaaggca
    25201 ctggctgcgc ggtgttttcc ttcagcggga actgataaga ggcgatagaa tgcccggctt
    25261 cgctgtagcg ggtgcgggaa tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt
    25321 ccagctgcgg cagcgtgatc tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt
    25381 taatctgctg cgtcgcgagc gtttcacctt cggcgcgcac ctctgcgtgc agggtgtagt
    25441 catcaagcgt ggtaaaccac agtttatttt cgactttcaa ctcgccgcga gtcagcgcgt
    25501 ggatttttac cggcgcgata acctgtttgt actctttcag gcccgatccc ggcgtctgat
    25561 cggaatagat caaaccatca agacagaagt tatagttgtt gggatagtcg ccgccgaatt
    25621 tataccagac attgccgtgg tcgtcctgtg cctggatccc gtggtcgcac cactcccaga
    25681 cataatgacc ctgaatgcaa tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc
    25741 ccggtccgtt ccccatcgca tgagcatatt cacagatgat gcgcggcttc ggatgcgggt
    25801 attcaccaaa ctcattcatc agcggcacgc gggtgtacat ggtggaaata atatcgacca
    25861 cttcagcatc gcgatcttct tcgtaatgaa ccagtcgcgt gtcatccagc gctttcgccg
    25921 catggtacat cgcgcggatg ttacagccat agccggattc attgcccagc gaccagatga
    25981 tgatcgacgg atggtttttc tgtgcgtgga tatggcgaac aatgcgctcg acgtagactt
    26041 tttcccactg cggatcgtcg gtaatacggc tgatatcgcc gacattagca aagccgtgcg
    26101 attcgacgtc ggtttccgcc atcacaaaca ggccgtagat atcacacagt tcgtaaaaac
    26161 gcggatcgtt cgggtagtga gcggtacgca cggagttgat attgtgctgc ttcatcaact
    26221 ggagatcttt ctcgacgcga tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt
    26281 gacggttgac gccgtgcagc atcacataac ggttattgat ccagaacaga ccgtcgcgca
    26341 ctttgatatc acggaagcca acgcgttgtg gcaccacttc cagaacgttg ccgttggcgt
    26401 ctttcagcgt catgaccaga tggtaaagat aaggggattc tgctgaccat tgctgcggct
    26461 gttcgacagt aaaagcaaag ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg
    26521 cgctgctgtg caccacgcgt tcgccatcaa acagggtata ttccagcgtc gtgacgacag
    26581 gggaggcggc gagattttcc agcaccactt cgcaggaaag cgtggcatcg caataggctt
    26641 cgtcaaagtc ggtacgcaca gtgaaatcgt taatatgcgt taggtgtttt ccgaccagat
    26701 aaacatcgcg gaagatcccc gccgaccacc acatatcctg gtcttccacg taggtagagt
    26761 ccgcccactg catcacgcgc acacacaaca ggttgtcgcc ggttttaacc atcgcgctga
    26821 tgtcaaactc tgcggtcagg cgactgccct tgctgaaacc cacatactga ccgttaacgt
    26881 agacttcaaa ataggtttcg acgccgtcaa atttaatcag cgtctgttta ccctgccagc
    26941 cgtcgctgag ggtgaaaata cgttgatagg caccggttgg gttatcgctg gggacaaacg
    27001 gcacatcgat ggggaacgga aaaccttcgt cggtatattg cagtttgccg tgaccttcca
    27061 tttgccacat ggcggggacg gtaatatgcc cccagtcagc cattaactca gaggtgaagg
    27121 cttctggtac ttgcagcgga tggtcaaaaa agtggaaatt ccactgactg cttaagggca
    27181 gaaacaggct gctggtttcg cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa
    27241 aaaagtacgc acgcggcgca agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat
    27301 tcatagggcc tcccggtaga gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa
    27361 ttgctggggg gttagtaaaa tttttactaa aaagcagcag gtaaatgcat ttactttaac
    27421 tggatcacga aaataaacgg cagcactgtc cggcaaaaat gattgccgga cagtgtgtaa
    27481 ggggatttaa cgggtcgtgc cgcgcagttt taatttgctg ggaacgaaga ctagcagcgg
    27541 cagcgcgcga ccatcgcggg ctttttcata caccaggtta acgccctgac ttcccatcat
    27601 ctcggaatgg atgcgcacgg tggagagcgg cggaaaggta aatcgcgcgg tggggatatc
    27661 gttaacgctg ataagcgaaa tatcttgtgg gatgttcagg ccacgttcat gaattgcccg
    27721 cagtacgccg atagcaatgg aatcggaagc aacaaacagt gccttcggat agtcttcccg
    27781 cgccagcatt tgttttgcca gttcataacc cgacgaactg gaaaaaccgc cgcgccagat
    27841 atcctcttcg cgtaccactt gtttcagtcg gccatattcc gcaaaggcga cctcacgaat
    27901 atccgccttg ccaggctcat cttcaccgcc aataaaacca atacgattaa cgccctggtt
    27961 gatatagaag tcgatgattt ctttactgat gcgtgccaga tcgatatcca ccgcatcgta
    28021 accgctgccg ggttcgtgaa agtcgataaa acagatattg tcggtcaacg cgctggcagc
    28081 ggcgcgcagg gcgggcgtgg gtttgccgac aattaaaata ccggtgacgt ttttaatgtc
    28141 tggtaatccg ttgtgttcat aacagttggt cagttcgatc gccagctttt cgcactgagt
    28201 ttcaatgccg tggcggatcg ccagatagta aggatcgttg atctccagct cctgctggta
    28261 gctgtagata gccagaatat ggtgttggtt gactgcgcct gtctgtagtt tacgggcgct
    28321 actggtcttg tactccagct tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt
    28381 cacattcaat gtcggatcgt catttaagac cctggatact gtcgccaggg atacgccagc
    28441 ttcgattgcg atgtctttta atgttgccat gtttaccttt tttactttcc ctgatgtgcg
    28501 cccccattgt agaggaagtg cttatcgggt cacctgtttt cagtaaataa agcgcgtttc
    28561 tttgacgctg taacggtaac acagcggctt aggaatgtga tcacgcctgc attctccatt
    28621 tttagcctac aggattgaga aaaacttttc tatagtttag taaaatttta tccgaaggga
    28681 ggaagcaatg gaaaccgtgg cttacgctga ttttgcacgt ctggaaatgc gcgtcggaaa
    28741 gattgtggaa gtgaaacgcc atgaaaacgc cgacaagctg tacatcgtac aggttgatgt
    28801 ggggaaaaaa cactgcaaac cgtgaccagc ctggtgccgt actacagcga agaggagctg
    28861 atggggaaaa cggtggtggt cttatgcaat ctgcaaaaag cgaaaatgcg tggtgaaacg
    28921 tcggaatgca tgctgttgtg tgcggaaacc gatgacggca gtgaaagcgt gttgttaacg
    28981 ccggagcgga tgatgcctgc gggcgtacgc gtcgtataaa aagatcggat ggcgacgtcg
    29041 tatcgccatc cgatttgata ttacgcttct tcgacactta ctcgcatggc cgccagcgcc
    29101 ttacgcgccg ctttgatcac cagttcacac tgttcaatgg tcagtgtcag tggcggttca
    29161 atgcggatcg ttttggcgtt attgagcgtt ccggccacca gtacgcgctg gcggaacatc
    29221 tcgctggcaa agttatagcc gatttcgtta tcaacaaact caatcgccat caacatccct
    29281 ttaccacgcg cttcctgtac cagatcggga tattcccgcg ccagttgacg gaaaccgtcc
    29341 agcaacatat cgcctttttg ctcagcctga gccggtaagt tctgctccag caacacattg
    29401 atggtcgcca gcgccgccgc acaggccagc gggttgccgc caaaggtggt ggtatgcagg
    29461 aatgggttgt cgaacagaac tgaaaacacc tcttcagtgg cgatggtcgc gccaatcggc
    29521 atcacgccgc cgccgagggc ttttgccaga cacagaatgt ccggctgaac gttctcatgt
    29581 tcgcaggcaa acatcttgcc cgtgcgcccc atgcccgttt gtacttcatc gaggatcatc
    29641 agtgcgccga actcatcgca cagcttacgc acggcggtga gatagcccgg cggcggcaga
    29701 attacgccac cttcgccctg aatcggttcg aggatcaccg cagccacatc atcaccggtt
    29761 tttttgcact cattaagagc cgtgcgcatg gcttcgatat tgccaaacgg cacatgacgg
    29821 aaccctggca gcagtggcat aaacggtttg cggaaggtcg atttcgccgt ggccgacagc
    29881 gcgccaagtg atttaccgtg gaacgcgccg ctggtggcaa taaaagtaaa tttgccgcgc
    29941 ggcgactgat aagccttcgc cagcttcagc gccgcttcaa cggactcggt gccgctatta
    30001 cagaagaagc tgtatttcag tttaccgggc gttagcgcag caagggtttt cgccagcata
    30061 gcccgtaacg gatcgagtag ctcctggctg tgcagtggtt gtttcgcaag ttgattctgt
    30121 acggcggaaa ccacaactgg attacggtgc cccacgttga aaattccaaa acctcccagg
    30181 cagtcgataa actcctgtcc ctgggtgtcg acaagcgtat ttaaacctcc cgcttgccac
    30241 tctacggctc cgtaatcccc gccggcggta acagatttgc gatactctaa aaaccccgga
    30301 ttgacatgct ctttgaagta ttcaatcacc tctcggttaa gtgctttcat ctcctcatga
    30361 tccagcgttc gcttctcaat gagattcagg gcgtgggcgc tgcacgctaa agccgatgcg
    30421 ctcgaaggta acctgttcaa aatatgctcc ggaggctcgc gtatcacatg ataccgattt
    30481 aagtattgca gggattgcgc cactccggct gcgatcgcga aaatcgggat aaacacaaat
    30541 gcggttatca tttgtgcaat atttaatgct atccgttaac atttgttgtt attttgcacg
    30601 gtttttgtcg ggtatggatt tgatgattgc tgcgcaaatg cacgttttca gggcaataat
    30661 tgcctgattt ttgggcaaga taattaacgt tttcgtatag taacagagtg aaagtatttt
    30721 aaatcaaaga attagatttg tgctttaatc gcaaattgcg atctaaatca aattaatcgg
    30781 ttaaagataa ccgcagcggg gccgacataa actctgacaa gaagttaaca accatataac
    30841 ctgcacagga cgcgaacatg tcttctcatc cgtatgtcac ccagcaaaat accccgctgg
    30901 cggacgatac cactctgatg tccactaccg atctgcaaag ctatatcact catgctaatg
    30961 acacttttgt gcaggtgagc ggctttacct tgcaagagtt acaagggcag ccgcacaaca
    31021 tggtgcgtca cccggatatg ccaaaagcgg cgtttgcgga tatgtggttc accctgaaaa
    31081 aaggggagcc ctggagcggc atcgtgaaaa atcgccgcaa aaatggcgac cattattggg
    31141 tgcgggccaa tgcggtaccg atggtgcgcg agggaaaaat cagtggctat atgtcgattc
    31201 gtacccgggc gacggatgaa gagattgcgg cggtggagcc gctgtacaaa gcgttgaact
    31261 ccggacgtac cagtaagcgt attcataaag gcctggtggt gcgtaaaggc tggctgggta
    31321 aactgccttc attaccgctt cgctggcgga cgcgtggagt gatgaccctg atgtttatct
    31381 tgctggcggc catgctttgg tttgttgctg ccccggtggt gacgtatttc ctctgtgtgt
    31441 tagtggtatt gttggcaagc gcttgttttg aatggcagat tgtgcgcccg atagaaaatg
    31501 tcgcccgtca ggcactgaag gtggcgaccg gagagcgtaa tagtgttgag cacctgaatc
    31561 gcagcgatga gctggggctg acattacgcg cggtagggca gcttggcctg atgtgccgtt
    31621 ggctaattaa cgatgtctca agccaggtgt ccagtgtcag aaatggcagt gagacgctgg
    31681 cgaaaggcac cgatgaactg aacgaacata cccagcagac agttgataac gttcagcaaa
    31741 cggtggcgac catgaaccaa atggcggcgt cggtgaaaca gaactctgcc acggcgtcgg
    31801 ctgccgataa actttctatc accgccagta atgcggcagt gcagggtggg gaggcgatga
    31861 ccacggtgat caagacaatg gacgatatcg ccgacagtac ctagcgcatt ggcaccatta
    31921 cttcgctgat taacgatatt gcgtttcaga ccaatattct ggccctgaat gcggcggtgg
    31981 aagcggcgcg tgccggcgaa cagggcaaag gttttgcagt ggtggcaggg gaagtgcgtc
    32041 atttagccag ccgcagcgct aatgctgcca acgatattcg caagctgatt gatgccagtg
    32101 ctgataaggt gcaatccggt tcgcagcagg tacacgccgc cggacggacg atggaagata
    32161 ttgtggcaca ggtgaaaaac gtcacccagt tgatcgccca gattagccat tcaacgctgg
    32221 aacaggccga tgggctttcc agcctgaccc gtgcagtgga tgagcttaac ctgatcaccc
    32281 agaaaaatgc cgagctggtg gaagagagtg cgcaggtgtc ggcgatggtg aaacaccgcg
    32341 ccagccgact ggaagacgcg gtgacggtgc tgcattaatc ggtatgccgg atccggcggt
    32401 tggagcacaa tgcctgatgc gatgctggcg catcttatca ggcctgcggg gtgtgtagcg
    32461 ggtgtaggcc ggataaggcg cgtcaagcgt cgcatccggc aattgcaccg cgccactggc
    32521 ggatgcggcg attaatccag ttgcgtaata tcaaaagcgg cgcggtcgat caccgcaatg
    32581 atctttttta tttgtgcatc actgatatcg ctctggttga cgcgtaaatc cagcaccgct
    32641 ttaaaattat ccagcgcccg cttcatttgc gggttctggc gcagcgccgc gccaacgcaa
    32701 cgcgctttga tgcgttcttc aatcatctcc acttgttcgc ggttttcttc cagccactgc
    32761 gcaccttgtt cggtcagcgc aatctgcttc ttaccacctt cctcttcgcg gatggtaatc
    32821 agcgactgct cctgcagaaa atccagcgtc gggtagatga cgcccgggct tggggtgtaa
    32881 ttcccctggg ttagattctc aatcgcttta atcaattcgt aaccgtggct gtcatcgcgc
    32941 gagagaatat ccagaatcac cagacgcaat tcaccgtgac caaagaaacg ttgccgacga
    33001 ccgccgccgc gaccgtggcg accaccgcaa cattgaccgt gttcatgctg gtgaccgtgt
    33061 ccgcagtgct cgtgttctga cttctcacct ttgcagcagc cctcatgccg tggctggcct
    33121 tcatgtttac aacacccttc gtgatgatgg ctcatatcag cctccttttg cttgattaga
    33181 tatatctaat ttatatctga tgtgagcatt actgcaagta caaaaatgat ttttaaacgt
    33241 ggaagcacct cacatctagg ccggataaga cgttcacgcc gcatccggca gcattgctat
    33301 ggcactgaat gccttcacat acttcagaca tatcagttct acaaatcgct tgcatttatc
    33361 atgattaaca atcattatca tttgcgaatt ttatttagat atatctgatt accatcacga
    33421 aggcgataac aatgaataac accccccgct acccgcagcg cgttcgcaat gatctgcgct
    33481 tccgtgaact gactgtgcta cgcgttgagc gcatcagcgc cggttttcag cgcattgtcc
    33541 ttggcggcga ggcgttggac ggttttacat cgcgcggctt tgacgatcac agcaaactct
    33601 tctttcctca acctgacgct cactttgtgc cgccaacggt aacggaagag agcatcgtct
    33661 ggccggaagg accacgccca ccgtcgcgtg actatacgcc actgtatgac gaactacgcc
    33721 atgaactggc gatcgatttc tttattcacg acggtggggt cgccagcggc tgggcgataa
    33781 acttacggtg gcaggtccgc gcggttcgct ggtggtgccg gaagattacg cgtatcagct
    33841 gtatgtctgc gatgaatccg gaatgcccgc actgcgccgc cgcctggaaa cgttgagcaa
    33901 acttgccgtt aaaccgcaag ttagtgcgct ggttagcgtg cgggataacg cctgtcagga
    33961 ttatctcgcg caccttgatg gttttaatat cgaatggctg gcacatgatg agcaggcggt
    34021 agatgcgcgt ctggcgcaga tgcaaatccc tgccgatgat tacttcatct ggataaccgg
    34081 cgaaggtaaa gtcgttaaga atttaagccg ccgctttgaa gcggaacagt atgacccaca
    34141 gcgggtacgt gcagcggctt actggcacgc aaagtaactg atatataaaa agaaaaaggc
    34201 tgacgatttc tcgtcagcct ttgctatatc tggtggcccc tgctggactt gaaccagcga
    34261 ccaagcgatt atgagtcgcc tgctctaacc actgagctaa ggggccatgg tagcggatta
    34321 taaagtaact ccgtgtcgca atccagccat taccgcgcgc ctgctgtttt tataaacaat
    34381 gtattttcaa tccgttatac ttttcctggt gatgttagaa aggagtaaat atggttgagg
    34441 atattttggc tccagggtta cgggtcgtgt tttgcggtat caaccctggg ctttcatccg
    34501 ccgggactgg ttttcccttt gctcatccgg caaatcgctt ctggaaggtg atatatcagg
    34561 ccgggtttac cgaccgtcag ttgaagccgc aggaggcaca gcatctgctg gattatcgtt
    34621 gtggcatcac caaactggta gaccgtccaa cggtgcaagc caatgaagtt tcaaagcagg
    34681 agctgcacgc aggtgggcgt aagctgattg aaaaaattga agattatcag ccgcaggcgt
    34741 tggcgattct gggcaaacaa gcatatgaac agggattcag ctagcgcggt gcacagtggg
    34801 ggaaacaaac gctcaccatt ggttcgacgc agatttgggt gctgccaaat cccagcggtt
    34861 taagtcgcgt ttcactggag aaactggttg aagcgtatcg cgagctggac caggcgctgg
    34921 tagtgcgtgg gcgataa
//
LOCUS       DAJVRL010000033        34467 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVRL010000033 DAJVRL010000000
VERSION     DAJVRL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.033"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..333)
                     /locus_tag="OQN02_001631"
     CDS             complement(<1..333)
                     /locus_tag="OQN02_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642467.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            703..1653
                     /gene="corA"
                     /locus_tag="OQN02_001632"
     CDS             703..1653
                     /gene="corA"
                     /locus_tag="OQN02_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1642468.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1694..2074)
                     /locus_tag="OQN02_001633"
     CDS             complement(1694..2074)
                     /locus_tag="OQN02_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCS1642469.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2088..2504)
                     /locus_tag="OQN02_001634"
     CDS             complement(2088..2504)
                     /locus_tag="OQN02_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642470.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="OQN02_001635"
     CDS             complement(2563..3453)
                     /gene="rarD"
                     /locus_tag="OQN02_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1642471.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3505..3972)
                     /locus_tag="OQN02_001636"
     CDS             complement(3505..3972)
                     /locus_tag="OQN02_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1642472.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4137..5006
                     /gene="pldA"
                     /locus_tag="OQN02_001637"
     CDS             4137..5006
                     /gene="pldA"
                     /locus_tag="OQN02_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1642473.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5139..6968
                     /gene="recQ"
                     /locus_tag="OQN02_001638"
     CDS             5139..6968
                     /gene="recQ"
                     /locus_tag="OQN02_001638"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1642474.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7032..7652
                     /gene="rhtC"
                     /locus_tag="OQN02_001639"
     CDS             7032..7652
                     /gene="rhtC"
                     /locus_tag="OQN02_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1642475.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="OQN02_001640"
     CDS             complement(7714..8334)
                     /gene="rhtB"
                     /locus_tag="OQN02_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1642476.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8445..9467
                     /gene="pldB"
                     /locus_tag="OQN02_001641"
     CDS             8445..9467
                     /gene="pldB"
                     /locus_tag="OQN02_001641"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1642477.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9475..10275
                     /gene="yigL"
                     /locus_tag="OQN02_001642"
     CDS             9475..10275
                     /gene="yigL"
                     /locus_tag="OQN02_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1642478.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10351..11250
                     /gene="bioP"
                     /locus_tag="OQN02_001643"
     CDS             10351..11250
                     /gene="bioP"
                     /locus_tag="OQN02_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1642479.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11138..12091)
                     /gene="metR"
                     /locus_tag="OQN02_001644"
     CDS             complement(11138..12091)
                     /gene="metR"
                     /locus_tag="OQN02_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1642480.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12327..14588
                     /gene="metE"
                     /locus_tag="OQN02_001645"
     CDS             12327..14588
                     /gene="metE"
                     /locus_tag="OQN02_001645"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1642481.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14627..15442)
                     /locus_tag="OQN02_001646"
     CDS             complement(14627..15442)
                     /locus_tag="OQN02_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1642482.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15704..16465
                     /gene="udp"
                     /locus_tag="OQN02_001647"
     CDS             15704..16465
                     /gene="udp"
                     /locus_tag="OQN02_001647"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1642483.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16606..18033
                     /gene="rmuC"
                     /locus_tag="OQN02_001648"
     CDS             16606..18033
                     /gene="rmuC"
                     /locus_tag="OQN02_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1642484.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18128..18883
                     /gene="ubiE"
                     /locus_tag="OQN02_001649"
     CDS             18128..18883
                     /gene="ubiE"
                     /locus_tag="OQN02_001649"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1642485.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18897..19502
                     /gene="ubiJ"
                     /locus_tag="OQN02_001650"
     CDS             18897..19502
                     /gene="ubiJ"
                     /locus_tag="OQN02_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1642486.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19499..21139
                     /gene="ubiB"
                     /locus_tag="OQN02_001651"
     CDS             19499..21139
                     /gene="ubiB"
                     /locus_tag="OQN02_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1642487.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21218..21487
                     /gene="tatA"
                     /locus_tag="OQN02_001652"
     CDS             21218..21487
                     /gene="tatA"
                     /locus_tag="OQN02_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1642488.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21491..22006
                     /gene="tatB"
                     /locus_tag="OQN02_001653"
     CDS             21491..22006
                     /gene="tatB"
                     /locus_tag="OQN02_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1642489.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22009..22785
                     /gene="tatC"
                     /locus_tag="OQN02_001654"
     CDS             22009..22785
                     /gene="tatC"
                     /locus_tag="OQN02_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1642490.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22827..23609
                     /gene="tatD"
                     /locus_tag="OQN02_001655"
     CDS             22827..23609
                     /gene="tatD"
                     /locus_tag="OQN02_001655"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1642491.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="OQN02_001656"
     CDS             complement(23606..24094)
                     /gene="rfaH"
                     /locus_tag="OQN02_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1642492.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24261..25754
                     /gene="ubiD"
                     /locus_tag="OQN02_001657"
     CDS             24261..25754
                     /gene="ubiD"
                     /locus_tag="OQN02_001657"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1642493.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25800..26501
                     /gene="fre"
                     /locus_tag="OQN02_001658"
     CDS             25800..26501
                     /gene="fre"
                     /locus_tag="OQN02_001658"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1642494.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="OQN02_001659"
     CDS             complement(26693..27856)
                     /gene="fadA"
                     /locus_tag="OQN02_001659"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1642495.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="OQN02_001660"
     CDS             complement(27866..30055)
                     /gene="fadB"
                     /locus_tag="OQN02_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1642496.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30245..31576
                     /gene="pepQ"
                     /locus_tag="OQN02_001661"
     CDS             30245..31576
                     /gene="pepQ"
                     /locus_tag="OQN02_001661"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1642497.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31576..32190
                     /locus_tag="OQN02_001662"
     CDS             31576..32190
                     /locus_tag="OQN02_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1642498.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32229..33680
                     /gene="trkH"
                     /locus_tag="OQN02_001663"
     CDS             32229..33680
                     /gene="trkH"
                     /locus_tag="OQN02_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1642499.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33692..34237
                     /gene="hemG"
                     /locus_tag="OQN02_001664"
     CDS             33692..34237
                     /gene="hemG"
                     /locus_tag="OQN02_001664"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1642500.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8112 a         8707 c         9313 g         8335 t
ORIGIN      
        1 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
       61 gccgttcatc gccatctgca cctgaccctg actattaata tccgccagca gagcatgtaa
      121 cgttccccac gcttcgccat tggctttttg ccagtacatt ttcacccgct ctgtttgagg
      181 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggt
      241 aacagcaaac aagggaagta aggtgagggt gagaaaaatc cgtttgagat tcaaggtgat
      301 cattccttta ccaatgagta gccgatgcgc cattataggt cctagatgtg ggattttttt
      361 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
      421 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
      481 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
      541 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
      601 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
      661 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
      721 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
      781 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
      841 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
      901 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
      961 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
     1021 cgtgaactgc ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac
     1081 ggtaacgcct acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat
     1141 gaaattgaaa atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat
     1201 cagggcgatg agtacgacga ggcgctctcc acgctggcgg aactggaaga tatcggctgg
     1261 aaagttcgcc tgtgtctgat ggatacccag cgcgctctca acttcctggt gcgtaaagcg
     1321 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgcgatat cgaatccctg
     1381 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcaatgggt
     1441 tttatcaaca tcgagcagaa ccgcatcatc aaaattttct cggtggtatc cgtcgtgttc
     1501 ctgccgccaa cgctggttgc ttccagctac gggatgaact ttgagtttat gccagaactg
     1561 aagtggagtt tcggctaccc tggcgcgatt atctttatga tcctcgcggg cctggcaccg
     1621 tatctgtact ttaagcggaa gaactggttg taaaaagaag gcggtggctt agcctggcta
     1681 agccacctgt tattcaaagg ctccaggtat ttaacccttt tacctctttc tcataaaacc
     1741 atttgttcgt gttaacagca acataggctg ctacggcaat tcccagaatg ttaacgccaa
     1801 ttaatgcacc aacaaataga gatagaatgc caagtaatag aactataatt gcttttttcc
     1861 ataagcccag aacaaataaa tagatccagg aacagaagaa agcaatgaaa ttcatttgaa
     1921 tagttaatcg ttgtcttact tttaatgctt taaatgctgc tttatattct ggtgttgctc
     1981 cccagaatcc aggaaagcca tgttgatcat aaaaattaaa tcggtatttc catttttcac
     2041 ttaatgaatc atcgttcata tattccttac tcataaatac tccataacta ttgttttgat
     2101 gaatcagtag gtgcaagcat tagcatactg aaagtggaaa aataacaaat caaaaaaatc
     2161 atcgaaccat tgcctgaaca ggcaaaatct tcggctatca ttgtgatgat agagatgata
     2221 tatactgcta atgtaccaaa aacataaatt tttatatata tgaaaccact atcacggagt
     2281 cgttggcaat tcatgttgat gacgagataa tggagtgcga tggtagagac tataacaaga
     2341 aaacctgctt ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa atttattaaa
     2401 ataaataaaa atatatatcg gtgtctggaa atcttaccgt tagatgttgg gataaacata
     2461 cgtaacataa attttacatc cttgtgtgag tctcctgtca gcatggcaat atgcccactc
     2521 tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg
     2581 ctgcgtataa atcgcgtcca tcacaaaaat tgccagcgcc acccaaataa aggtgaaagt
     2641 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
     2701 cgtcgggcca atgtactgaa aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc
     2761 agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag
     2821 cgacatcggg ttttgcccca tatggctggt tgaactgtcg gcaatagcaa acaggtaaat
     2881 cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc
     2941 aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat
     3001 aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca gaatcaccgc
     3061 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
     3121 cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
     3181 gataaacagt agccagttgc caccaatcag tacggcagag actgccagca taaaaatttt
     3241 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
     3301 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg cgggcacgta
     3361 gtaaatcagc ttgaagtatg ctggcgcaat accccaaata aaataagcgg caagagcgag
     3421 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     3481 agcagtttac ctgcttttat gtcttcaacc caccatatag gtggcggtgg cactggcaat
     3541 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     3601 caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg
     3661 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     3721 gtggcgggtt aaggtacttc ccacgcacac caggccggcg gcgacatcca gcgccgacgc
     3781 aatgactccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt
     3841 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     3901 aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag
     3961 tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt ttatgcttct
     4021 ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa
     4081 atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc
     4141 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     4201 aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata
     4261 tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct
     4321 acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg gcggaaaatg
     4381 cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccgctgtgg cgtgggattt
     4441 taggcccaaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca
     4501 atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt
     4561 ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc
     4621 acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc
     4681 tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata
     4741 ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc
     4801 acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg
     4861 gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt
     4921 acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtag
     4981 gggttatgct aaacgacttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca
     5041 gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa
     5101 aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga
     5161 atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc
     5221 ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg gttgttatgc
     5281 ccactggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta
     5341 ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctacaggcca
     5401 acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa cttgaagtga
     5461 tgacaggctg ccgcaccggg caaattcgct tactgtatat cgccccggaa cgcctgatgc
     5521 tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc gtcgatgaag
     5581 cgcactgtat ctcccaatgg ggccacgatt tccgcccgga atatgccgcg ctcggtcagt
     5641 tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca
     5701 cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt
     5761 ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga
     5821 tgcgctacgt gcaggaacag cgcggtaagt ctggcattat ctactgcaac agccgggcga
     5881 aagtagaaga caccgctgcg cgcctgcaaa gcaggggtat tagcgcggcg gcctatcatg
     5941 ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgatctgc
     6001 aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg
     6061 tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg
     6121 ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc
     6181 tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac
     6241 tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact
     6301 attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga
     6361 aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc
     6421 agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc
     6481 gcgactatgg tcatgacaaa ctgaaagtct atggtatggg ccgtgataaa agccatgaac
     6541 attgggtgag cgtgatccgc cagctgattc atctcggcct ggtgacgcaa aatattgccc
     6601 agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt
     6661 tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg
     6721 gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg
     6781 atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg
     6841 aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc
     6901 tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg
     6961 aagagtagtc agcagcataa aaaagtgcca gtatgaagac tccgtaaacg tttcccccgc
     7021 gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt gtggcgctta
     7081 tgagccccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag
     7141 aagcgatgat gggcgtgctg ggcattacct gcggcgtaat ggtttgggct gggattgcgt
     7201 tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggtag
     7261 gcggtggcct gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag
     7321 aggctgtatc tgcgcctgca ccacaggttg agctggcgaa aagcgggcgc agtttcctga
     7381 aaggcttact gaccaatctc gccaatccga aagcgattat ctattttggt tcggtattct
     7441 cattgtttgt cggcgataac gttggcacta ccgagcgctg gggcattttc gcgttgatta
     7501 ttgtcgaaac actggcgtgg tttaccgtcg ttgccagtct gtttgccctg ccgcaaatgc
     7561 gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg
     7621 gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg
     7681 ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag
     7741 cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg
     7801 tcctttaatc catagagcaa tcctttgagc aagggtggcg taaccgatca tcacaataat
     7861 atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg gctgttgcgg
     7921 catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt
     7981 cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag
     8041 atcaattgcg ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc
     8101 tgcccacttt aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac
     8161 gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc
     8221 gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga
     8281 caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa
     8341 ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac
     8401 gcttaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa
     8461 aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt
     8521 tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg
     8581 tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga
     8641 gctacgtaaa atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa
     8701 tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc
     8761 atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc
     8821 agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca
     8881 cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt
     8941 ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag
     9001 cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt
     9061 ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg
     9121 ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc
     9181 tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgcaaacg cttctcttac
     9241 aagctgaaga ggaacgcgtg gtggataacc gcatgcacga ccgtttttgt gaactccgca
     9301 ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aataaaaggt gcttaccatg
     9361 agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc gttgattttt
     9421 tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac
     9481 caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct
     9541 tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc
     9601 ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg
     9661 attacctcca atggtgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac
     9721 ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt
     9781 actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc
     9841 ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc cgggattact ggagccggaa
     9901 ggcgtcagca aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag
     9961 gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg
    10021 gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg
    10081 ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg
    10141 tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agatctgcat
    10201 ccggagctgg aagttattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa
    10261 ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat
    10321 ggatactcgt taatcaaaga gttttccatt gtggcgttac ttatcatcac cacgattctg
    10381 tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt
    10441 gcggtgctgg tgcgcgttgg cttggcggca ctcgtttttc tgccgtttct gcgtacccgt
    10501 agcaatagcc tgaaaacggt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg
    10561 atgtatatgc tgagtttccg cgcttatctc tacctgacgg tctccgaatt gttgctgttc
    10621 accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg
    10681 cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat tattcgctat
    10741 gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact
    10801 tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagtat
    10861 aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg
    10921 ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt
    10981 ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac
    11041 gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg
    11101 gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct ggtgatcctg
    11161 gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat
    11221 cgcaggcgtg attgcgcgct gagcgaataa acgcttccgt aactggctga cgctgctcgc
    11281 catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca
    11341 ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc
    11401 gcgcggcaac catttgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga
    11461 cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa
    11521 ttaatagcgt ctcgctggcg agatcttccg gtgtaatacg cgtttttgcc gccagtggat
    11581 ggtcaggtgc taacaccagg cgtacttcat aatcgaacat cggcgaataa tgcaggccac
    11641 tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct
    11701 gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga
    11761 aattttctaa agcgggtgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca
    11821 gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctgatttgc ggcagcacct
    11881 ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac
    11941 gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg
    12001 attgcgtctg atgcaacgtt gccgcagcgg ctgcgagcga gccgcagttc cgcaacgctt
    12061 gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt
    12121 tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa
    12181 aatcattcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc ggtcacttac
    12241 ttcagtaagc tcccggcgat gaataaactt gccgccttcc ctaaattcaa aatccatagg
    12301 atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc ggtttccctc
    12361 gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca
    12421 cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa
    12481 agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat catgtactga
    12541 ccaccagttt gctgctgggt aatgttccgc cacgtcatca gaacaaagac ggttcggtag
    12601 atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg
    12661 cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg gagttcgtta
    12721 aaggccaaca gttcaaactg acctggacgc agctgctgga ggaagtggac gaggcgctgg
    12781 cgctgagcca caacgttaag cctgtgctgc tggggccggt tacttacctg tggctgggga
    12841 aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa cgacattctg ccggtttatc
    12901 agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg
    12961 cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct tacgacgcgc
    13021 tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg ccaaatctcg
    13081 acacgattac tgcgctgcct gttcaggggc tgcatgtgga ccttgtacat ggtaaagata
    13141 acgttgttga actgcacaag cgcctgcctt ctgactggct gctgtctgcg ggtctgatca
    13201 atggtcgtaa cgtctggcgc gccgatctta ctgagaaata tgcgcaaatt aaggacattg
    13261 tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc cccatcgacc
    13321 tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc gccctacaaa
    13381 aatgccatga actggcactg ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg
    13441 cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat aatccggcgg
    13501 tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat gtctatgaag
    13561 tgcgtgctga agcccagcgt gcgcgtttta aactgccagc gtggccgacc accacgattg
    13621 gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc
    13681 tcgacgccaa caactaccgc acgggcattg cggaacatat caagcaggcc attgttgagc
    13741 aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat gacatggtgg
    13801 aatactttgg cgagcacctc gacggatttg tctttacgca aaacggttgg gtacagagct
    13861 acggttcccg ctgcgtgaag ccaccgattg tcattggtga cattagccgc ccggcaccga
    13921 ttaccgtgga gtgggcgaag tatgcgcaat cgctgaccga caaaccggtg aaagggatgc
    13981 tgacggggcc ggtgaccata ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa
    14041 ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg
    14101 gaattggcat catccagatt gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta
    14161 gcgactggga tgcgtatctc cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg
    14221 cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac gacatcatgg
    14281 attcgattgc ggcgctggac gcagacgtca tcaccatcga aacctcgcgt tccgacatgg
    14341 agttgctgga gtcgtttgaa gagtttgatt atccaaatga aatcggtcct ggcgtctatg
    14401 acattcactc gccaaacgta ccgagcgtgg aatggattga agccttgctg aagaaagcgg
    14461 caaaacgcat tccggcagag cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg
    14521 gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag aatttgcgtc
    14581 gggggtaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac agcgacttct
    14641 tcccaccata ctgcttaaac cattccagca tacgctgcca gccatctttc gcagattcgg
    14701 catgatagct cgggcgataa tcagcgttga atgcatgccc ggcatccggg tacacgataa
    14761 tctctgcttt cgcattagca gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg
    14821 gaatgctgtt atcctgacca ccatataagc cgagaaccgg cgcgttaaga tcggttgcga
    14881 tatcaacagg ttgtttcggt gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg
    14941 ccactgcggc ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc
    15001 agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg
    15061 cgacatgatc gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa
    15121 gcaacgtggg gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg
    15181 cgatagccag atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca
    15241 cgccaaaaat ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc
    15301 tggcatggta agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa
    15361 tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc
    15421 cagattgttg tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt
    15481 taactatagt cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc
    15541 ctgcagaatg aagagtgatt tatgtgatat gaatcacttt tggtgggtaa atttatgcaa
    15601 cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc
    15661 tttttgcttc ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt
    15721 ttttcatctc ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg
    15781 cgacccggat cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc
    15841 tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc
    15901 taccggtatc ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat
    15961 tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga
    16021 tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct
    16081 ggaattcccg gctgtcgctg atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc
    16141 tattggcgcg acaactcacg ttggcgtgac agtttcttct gataccttct acccaggtca
    16201 ggaacgttac gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga
    16261 gtggcaggcg atgggcgtaa tgaactatga aatggaatct gcaactctgc tgaccatgtg
    16321 tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc gcacccagca
    16381 agagatcccg aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt
    16441 ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc
    16501 cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt ccaactggac gtttgtacag
    16561 cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc
    16621 atggtttacg cagttattgc gttggtgggt gtggcaattg gctggctgtt tgccagttat
    16681 caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg aagagatggt cgcggagtta
    16741 agcgcggcaa aacaacaaat tacccaaagc gagcactggc gtgcagagtg tgaattgctc
    16801 aataacgaag tgcgcagcct gcaaagtatt aacacctctc tggaggccga tctgcgtgaa
    16861 gtaaccacgc ggatggaagc cgcacagcaa catgctgacg ataaaattcg ccagatgatt
    16921 aacagcgagc agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac
    16981 agcaatcgcc gggttgatga gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta
    17041 cgtgaacaac tggacggttt ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa
    17101 gaacgccata ccctgaccca cgaaattcgc aatctccagc aactcaacgc gcaaatggcc
    17161 caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg
    17221 ggcgaggtag tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat
    17281 gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg
    17341 cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa
    17401 cgctatttta acgccgaaga cgactacacc cgcgaaagcg cgctacagga acatatcgcg
    17461 tcggtgcgta accatatccg tttgctggga cgcaaagatt atcaacagct gccggggctg
    17521 cgaactctgg attacgtgct gatgtttatt cccgttgaac ccgctttttt actggcgctt
    17581 gaccgccagc cggagctgat caccgaagcg ttgaaaaaca atatcatgct ggttagcccg
    17641 actacgctgc tggtggcgtt gcgtactatt gccaacctgt ggcgttatga gcatcaaagc
    17701 cgtaacgccc agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc
    17761 atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag
    17821 gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc
    17881 ggtttaggag tagaaattaa acgcgagatt aatccggatt tggctgaaca ggcggtgagc
    17941 caggatgaag agtatcgact tcggtcggtt ccggagcagc cgaatgatga agcttatcaa
    18001 cgcgatgatg aatataatca gcagtcgcgc tagcccatta ggggtagtta agccgggtag
    18061 aaatctaggg catcgacgcc caatctgtta cacttctgga acaatttttt gatgagcagg
    18121 cattgagatg gtggataagt cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa
    18181 ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc gtggcatcaa aatacgatgt
    18241 catgaatgat ttgatgtcat ttggtattca tcgtttgtgg aagcgattca cgattgattg
    18301 cagcggcgta cgccgtgggc agaccgtgct ggatctggct ggtggcaccg gcgacctgac
    18361 agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg atatcaatga
    18421 atccatgctc aaaatgggac gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga
    18481 gtatgttcag gcgaacgctg aggcgctgcc gttcccggat aacacctttg attgcatcac
    18541 catttcgttt ggtctgcgta acgtcaccga caaagataaa gcactgcgtt caatgtatcg
    18601 cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc
    18661 gctgagcaaa gcctatgatg catactcctt ccatgtgctg ccgcgtattg gctcactggt
    18721 cgcgaacgac gccgacagct accgttatct ggcagaatcc atccgtatgc atcccgatca
    18781 ggataccctg aaagccatga tgcaggatgc cggattcgaa agtgtcgact actacaatct
    18841 gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc
    18901 cttttaaacc tttagtgacg gcaggaattg aaagtctgct caacaccttc ctgtatcgct
    18961 cacccgcgct gaaaacagcc cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa
    19021 aaggcttttc gacgtcattg attctggtgt tcagcgaacg ccaggttgat gtactgggcg
    19081 aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc
    19141 gcgatcgcca gcagcttacc gcactgattc gcagtggtga gctggaagtg cagggcgata
    19201 ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc agagttcgac cctgcggaac
    19261 tgctggcccc ttataccggt gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag
    19321 gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg ttatgtggcg gaagccatta
    19381 ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg
    19441 ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct ggaaaaactg gaggctaaat
    19501 gacgccaggt gaagtacggc gcctatattt catcattcgc acttttttaa gctacggact
    19561 tgatgaactg atccccaaaa tgcgtatcac cctgccgcta cggctatggc gatactcatt
    19621 attctggatg ccaaatcggc ataaagacaa acttttaggt gagcgactac gactggccct
    19681 gcaagaactg gggccggttt ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct
    19741 ttttccaccg catattgccg atcagctggc gttattgcag gacaaagttg ctccgtttga
    19801 tggcaagctg gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg
    19861 gtttgacgat tttgaaatca agccgctggc ttctgcttct atcgcccagg ttcataccgc
    19921 gcgattgaaa tcgaatggta aagaggtggt gattaaagtc atccgcccgg atattttgcc
    19981 ggtcattaaa gcggatctga aacttatcta ccgtctggct cgctgggtgc cgcgtttgct
    20041 gccggatggt cgccgtctgc gcccaaccga agtggtgcgc gagtacgaaa agaccttgat
    20101 tgatgaactg aatttgctgc gggaatctgc caacgccatt cagcttcggc gcaattttga
    20161 agacagcccg atgctctaca tcccggaagt ttaccctgac tattgtagtg aagggatgat
    20221 ggtgatggag cgcatttacg gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg
    20281 cactaacatg aaattgctgg cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg
    20341 cgacagcttt ttccatgccg atatgcaccc tggcaacatc ttcgtaagct atgaacaccc
    20401 ggaaaacccg aaatatatcg gcattgattg cgggattgtt ggctcgctaa acaaagaaga
    20461 taaacgctat ctggcagaaa actttatcgc cttctttaat cgcgactatc gcaaagtggc
    20521 agagctacac gtcgattctg gctgggtgcc accagatacc aacgttgaag agttcgaatt
    20581 tgccattcgt acggtctgtg aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg
    20641 acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag tgcagccgca
    20701 actggtgtta ctccagaaaa ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc
    20761 gcagctcgat ttatggaaaa cagcgaagcc tttcctggag tcgtggatta aagatcaggt
    20821 cggtattcct gccctggtga gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat
    20881 gccagaactg cctgaactgg tttacgacag tttgcgccag ggcaagtatt tacagcacag
    20941 tgttgataag attgcccgcg agcttcagtc aaatcatgta cgtcagggac aatcgcgtta
    21001 ttttctcgga attggcgcta cgttagtatt aagtggcaca ttcttgttgg tcagccgacc
    21061 tgagtggggg ctgatgcccg gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg
    21121 ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg
    21181 gctttgttta atcatcatct accacagagg aacatgtatg ggtggtatca gtatttggca
    21241 gttattgatt attgccgtca tcgttgtact gctttttggc accaaaaagc tcggctccat
    21301 cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc
    21361 aaagcaggat aaaaccagtc aggatgctga ttttactgcg aaaactatcg ccgataagca
    21421 ggcggatacg aatcaggaac aggctaaaac agaagacgcg aagcgccacg ataaagagca
    21481 ggtgtaatcc gtgtttgata tcggttttag cgaactgcta ttggtgttca tcatcggcct
    21541 cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg gctggattcg
    21601 cgcgttgcgt tcactggcga caacggtgca gaacgaactg acccaggagt taaaactcca
    21661 ggagtttcag gacagtctga aaaaggttga aaaggcgagc ctcactaacc tgacgcccga
    21721 actgaaagcg tcgatggatg aactacgcca ggccgcggag tcgatgaagc gttcctacgt
    21781 tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc atccataacc cggtggtgaa
    21841 agataatgaa gctgcgcatg agggcgtaac gcctgccgct gcacaaacgc aggccagttc
    21901 gccggaacag aagccagaaa ccacgccaga gccggtggta aaacctgctg cggacgctga
    21961 accgaaaacc gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa
    22021 gatactcaac cgcttatcac gcatctgatt gagctgcgta agcgtctgct gaactgcatt
    22081 atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat ctatcacttg
    22141 gtatccgcgc cattgatcaa gcagttgccg caaggttcaa cgatgatcgc caccgacgtg
    22201 gcctcgccgt tctttacgcc gatcaagctg acctttatgg tgtcgctgat tctgtcagcg
    22261 ccggtgattc tctatcaggt gtgggcattt atcgccccag cgctgtataa gcatgaacgt
    22321 cgcctggtgg tgccgctgct ggtttccagc tctctgctgt tttatatcgg catggcattc
    22381 gcctactttg tggtctttcc gctggcattt ggcttccttg ccaataccgc gccggaaggg
    22441 gtgcaggtat ccaccgacat cgccagctat ttaagcttcg ttatggcgct gtttatggcg
    22501 tttggtgtct cctttgaagt gccggtggca attgtgctgc tgtgctggat ggggattacc
    22561 tcgtcagaag acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg
    22621 atgttgctga cgccgccgga tgtcttctcg caaacgctat tggcgatccc gatgtactgt
    22681 ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa
    22741 gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag aataaattca accgcccgtc
    22801 agggcggttg tcatatggag tacaggatgt ttgatatcgg cgttaatttg accagttcgc
    22861 aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc
    22921 tactcatcac cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt
    22981 attcgtcctg ttggtcaacg gcgggcgtac atcctcacga cagcagccag tggcaagctg
    23041 cgactgaaga agcgattatt gagctggccg cgcagccaga agtggtggcg attggtgaat
    23101 gtggtctcga ctttaaccgc aacttttcga cgccggaaga gcaggaacgc gcttttgttg
    23161 ctcagctacg cattgccgca gaattaaaca tgccggtatt tatgcactgt cgcgatgccc
    23221 acgagcggtt tatgacattg ctggagccgt ggctggataa actgcctggt gcggttcttc
    23281 attgctttac cggcacacgc gaagagatgc aggcgtgcgt ggcgcgtgga atttatatcg
    23341 gcattaccgg ttgggtttgc gatgaacgac gcgggctgga gctgcgggaa ttgttgccgt
    23401 tgattccggc ggagaaattg ctgatcgaaa cagatgcgcc gtatctgctc ccgcgcgatc
    23461 tcacgccaaa gccatcatcc cggcgcaacg agccagccca tctgccccat attttgcaac
    23521 gtattgcgca ctggcgtgga gaagatgccg catggctggc tgccaccacg gatgccaatg
    23581 tcaaaacact gtttgggatt gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt
    23641 gcttaatctc tttattaata agattaagca atagcatgga gcgagcctca ccatcgggtt
    23701 cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt aataatcacc ttatcacccg
    23761 gataaggggg ggccggatcg acaatgtctt tcggtttata taccgatagc tgatgaatta
    23821 ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac aaagtggctg acgccacgcg
    23881 tagcgctgat ggtcgtggta tgaatcactt ctggatcaaa ttccacaaac aggtagttag
    23941 ggaataacgg ttcactgact gcggtacgtt taccccgtac gattttttcc agggtgatca
    24001 tcggagcgag gcaattaacc gcctgtctct caaggtgttc ctgggcacgt tgaagttgcc
    24061 cgcgcttgca gtacagtaaa taccaggatt gcataatgac tcttatccgt ttaatcgggg
    24121 cgcaaggata gcaaaagctt tacgctaagt taattatatt ccccggtttg cgttataccg
    24181 tcagagttca cgctaattta acaaatttac agcattgcaa agatgaacgc cgtataatgg
    24241 gcgcagatta agaggctaca atggacgcca tgaaatataa cgatttacgc gacttcctga
    24301 cgctgcttga acagcagggt gagctaaaac gtatcacgct cccggtggat ccgcatctgg
    24361 aaatcactga aattgctgac cgcactctgc gtgctggtgg gcctgcgctg ttgttcgaaa
    24421 accctaaagg ctactcaatg ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg
    24481 cgatgggcat ggggcaggaa gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt
    24541 ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt
    24601 ttaagcaggt gttaaacatg ccgacaaagc gactgcgtgg tgcgccctgc caacaaaaaa
    24661 tcgtctctgg cgatgacgtc gatctcaatc gcattcccat tatgacctgc tggccggaag
    24721 atgccgcgcc gctgattacc tgggggctga ccgtaacgcg cggcccgcat aaagagcggc
    24781 agaatctggg catttatcgc cagcagctga ttggtaaaaa caaactgatt atgcgctggc
    24841 tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg tgcggcgcat ccgggcgaac
    24901 atttcccggt ttctgtggcg ctgggtgccg atcctgccac gattctcggt gcagtcaccc
    24961 ccgttccgga tacgctttca gagtatgcgt ttgccggatt gctacgcggc accaaaaccg
    25021 aagtagtaaa gtgtatttcc aatgacctcg aagtgccagc cagtgccgaa atcgtcctgg
    25081 aagggtatat cgatcctggt gagatggcgc cggaagggcc gtatggtgac cacacaggtt
    25141 actataatga agtcgatagt ttcccggtgt ttaccgtgac gcatattacc cagcgtgaag
    25201 atgcgattta ccattccacc tataccgggc gtccgccaga tgagcccgcg gtactgggcg
    25261 tggcgttgaa cgaagtgttt gtaccgattc tgcaaaaaca gttcccggaa attgtcgatt
    25321 tttacctgcc gccggaaggc tgttcttatc gtctggcggt agtgacgatc aaaaaacagt
    25381 acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt
    25441 acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga
    25501 tttgggcgat taccacccgt atggacccgg cgcgggatac tgttctggta gaaaatacgc
    25561 ctattgatta tctggatttt gcctcgcctg tctctgggct gggttcaaaa atggggctgg
    25621 atgccacgaa taaatggccg ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag
    25681 atccagatgt tgtcgcgcat attgacgcca tctgggatga actggctatt tttaacaacg
    25741 gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc gatccgacag agaaagcgca
    25801 tgacaacctt aagctgtaaa gtgacctcgg tagcagctat cacggatacc gtatatcgtg
    25861 tccgcatcgt gccagacgcg gccttttctt ttcgtgctgg tcagtatttg atggtagtga
    25921 tggatgagcg cgacaaacgt ccgttctcaa tggcctcgac gccggatgaa aaagggttta
    25981 tcgagctgca tattggcgct tctgaaatca acctttacgc gaaagcagtc atggaccgca
    26041 tcctcaaaga tcatcaaatc gtggtcgacc ttccccacgg agaagcgtgg ctgcgcgatg
    26101 atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg gttctcttat gcccgctcga
    26161 ttttgctgac agcgttggcg cgtaacccaa accgtgatat caccatttac tggggcgggc
    26221 gtgaagagca gcatctgtat gatctctgcg agcttgaggc gctttcgtta aagcaccttg
    26281 gtctgcaagt ggtgccggtg gttgaacaac cggaagcagg ctggcgtggg cgtactggta
    26341 ccgtgttaac ggcggtattg caggatcatg gtacgctggc agagcatgat atctatattg
    26401 ccggacgttt tgagatggcg aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc
    26461 gggaagatcg cctgtttggc gatgcgtttg catttatctg agatataaaa aaacccgccc
    26521 ctgacaggcg ggaagaacgg caactaaaac tttattcagt ggcatttaga tctatgtcgc
    26581 atccggcaat tggtgcacga tgcctgatgc gacgctggcg cgtcttatca ggcctacatt
    26641 ggtgccggat cggtagaccg gataaggcgt tcacgccgca tccggcaagt ggttaaaccc
    26701 gctcaaacac cgtcgcaata ccctgaccca gaccgataca catcgtcgcc agaccaaact
    26761 gaacgtcttt gcgttccatc agattcagca gcgtggtgct gatacgcgca ccggaacaac
    26821 ccagcggatg acccagcgcg atcgcaccac cattgaggtt gatcttctcg tcaatctgct
    26881 ccattagccc gagatcttta atgcacggca ggatctgcgc ggcaaaggct tcgttcatct
    26941 caaacacgcc gatatcgctg gcagaaagcc ccgctttttt cagcgccagc ttcgaggccg
    27001 gaaccgggcc gtaacccata atcgatgggt cacaaccaac gaccgccatc gaacgcacac
    27061 gagcgcgcgg cttaagacct aattcatggg cgcggctttc actcatcacc agcatggcag
    27121 ctgcgccatc ggaaagtgca gaagatgtgc ccgccgttac cgtaccgttt actggatcaa
    27181 acgccggacg cagcgtggcg agggcttcca cggtggtttc cgggcgaatc acttcgtcgt
    27241 aattaaactg cttcaggacg ccgtcggcat cgtgaccacc agttgggatg atttcatttt
    27301 taaatgcggc cgactgcgtg gcggcccagg cgcgggcgtg tgaccgcgcg gcaaaggcat
    27361 cctgcatttc acggctgata ccgtgcatac gcgccagcat ttctgccgtt aagcccatca
    27421 tgcccgccgc tttggcgaca ttgcggctca ggccggggtg aaaatcgacg ccgtgactca
    27481 tcggcacatg gcctatatgc tccacgccgc caaccagaca tgcctgcgca tcgccagtca
    27541 tgatcattcg tgctgcgtca tgcagtgcct gcatggatga accacacaag cgattgacgg
    27601 taaccgccgg gacagagtgc ggcacttctg ccagcagcgc cgcgttacgg gcgatgttaa
    27661 aaccctgctc cagcgtctgt tgcacacaac cccagtaaat atcgtcgagg gccgccgctt
    27721 ccagcgccgg gttacgcgcc agcaggctac gcattaaatg agcggagaga tcttccgcac
    27781 gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa
    27841 tgacaacctg ttccattgtg actccttaag ccgttttcag gtcgccaacc ggacgggctg
    27901 gctcaaccgg aggatagtac ggttcgttat gacgcgcttt attacgcaga ccttccggca
    27961 cttcatacag cgggccgagg tgctgatatt gctgtgccat atcgaggtat tttgcgctac
    28021 cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg agggaagccc aggccgtaga
    28081 ccagcgccat atccgcttcc gccggagtgg cgataatgcc ctcttccaga cagcgcacca
    28141 cttcgttgac catcgggatc atcatgcggg cgataatctc ttcttcgctg aaatcgcgct
    28201 tcggttggct cacttctgcc agcaggtctt caacggcggc gtcttcttct ttcttcggct
    28261 tacctttgct gtcttcttta taacgccaga aaccgaggcc gttcttctga ccaaagcggt
    28321 tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt ctgcatccgc tgcgggaagc
    28381 ctgctgccat gacagcctga gcgtgatgcg cggtatcaat gcccacaacg tccagcagat
    28441 atgccgggcc catcggccag ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat
    28501 ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata cgggaacagc acgcggttaa
    28561 caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt gcccatcttg ctcgcccagg
    28621 cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc gcgaataatt tctaccaacg
    28681 gcattcggtg gaccgggtta aagaagtgca tcccgcagaa gttttccggg cgttccagcg
    28741 cgttggccag ttcgctgata ggaatggttg aagtgttaga cgccagcacg gtatcctggc
    28801 gtactttttg ttcggtttct gccagtacgg cttttttcac tttcgggttt tcaacaaccg
    28861 cttctaccac aatatccacg cggtcaaatc cggcgtagtc gagcgttggg tggatcgtgg
    28921 agatcacgcc agccagtttc agaccatcga ttttgccgcg ctcaagctgc ttgttcagca
    28981 gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc gttgatatct ttcatgacaa
    29041 ccggcacgcc tttccacgcg gactggtaag cgataccgcc acccatgatg cctgcgccca
    29101 gcacagcagc ctgtttcggg gtttcaacgt ctttggtgag tttcttcgct ttgcctttta
    29161 catattgatc gttaaggaaa atgccgacca gtgcgcgagc ttcgttggta tgcgccagcg
    29221 ggacaaaact tttgttttcc agatttaagg cttcttcacg accaaaacgg gccgcagctt
    29281 caatggtttt tactgcggtg atgggggccg gataatgttt ccccgccgtt tgtgcgacca
    29341 ttcctttagc gatggtgaag ctcatggtgg cttcaatctt gctcagtttt agcggttcct
    29401 gcttcggctg acgtttcgct ttccagtcga ggtcgccgtt aatggcctgg cgtaaaatcg
    29461 ccatcgcccc ttcaatcagt ttttctgctt tgactacgcc atccaccaga ccgattttca
    29521 gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat ttccagtgca ctgtcagcgc
    29581 ccagcatacg tggcatacgt acagaaccgc caaagcccgg catgatgccc agtttggttt
    29641 ccggcagacc gatgcgcaga tccggcgtcg ccagacgata atcggtcgcc agcacgcatt
    29701 cgcagccacc acccagcgca tagccgttga cggcagcaat ggtcggcacc ggcagatctt
    29761 ccaggcgatt aaacacgcta ttggcaaagt gcagccactg acttaactgt tcttcaggaa
    29821 cgaggaacag ggacaaaaat tcggtgatat cagcaccgac gataaaggct gctttgttcg
    29881 aacgcagcag cagccctttt agatctgatt gctgttccag cacgccgatg gcctcgccga
    29941 ggctggcgac ggtcgcagtg tcgagtttat taactgaacc tggggcatca aataccagtt
    30001 cggcaatgcc atcttccagc cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag
    30061 tctcctgaat ccgcaaggtg atctggtcgt accagatgca tcggagtgtg tgttttgtgt
    30121 taaaaatatg caaataaaag attaaagaaa tgccgatctg atcacgctcg gcagaaatca
    30181 cgctctggat gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta
    30241 aaaaatggaa tcactggcct cgctctataa aaatcatata gctaccttac aagaacggac
    30301 tcgcgatgcg ctggcgcgct tcaagctgga tgcattactt attcactccg gcgagctgtt
    30361 caacgttttt ctcgacgatc atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg
    30421 ggtgccggta actcaggtgc caaactgctg gttgctggtg gatggcgtga acaagccgaa
    30481 actgtggttc tatctgccgg ttgattactg gcacaacgtc gaaccgctgc cgacctcctt
    30541 ctggactgaa gatgtggaag tgatcgcgct gccgaaagcc gatggcattg gtagcctgct
    30601 gcctgctgcg cgcggcaata tcggttatat cggtccggtg ccggaacgtg cgctgcaact
    30661 gggtattgag gccagcaaca tcaacccgaa aggggtgatc gactacctgc attattaccg
    30721 ctccttcaaa accgagtacg aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa
    30781 tggtcatcgt gcggcagaag aagcgttccg ttctggcatg agcgagttcg atatcaatat
    30841 tgcctatctg accgcgaccg gtcatcgtga taccgacgta ccttacagca acattgtggc
    30901 gcttaacgaa cacgctgcgg tgttgcatta caccaaactg gaccaccagg caccggaaga
    30961 gatgcgcagc ttcctgctgg atgccggggc cgaatataac ggctatgcgg ctgacctgac
    31021 ccgtacctgg tcggcaaaaa gcgacaacga ctacgcacag ctggtgaaag acgtaaatga
    31081 tgaacaactg gcgctgatcg ccaccatgaa agcaggcgtc agctatgtgg attaccacat
    31141 ccagttccat cagcgcatcg ccaaactgct gcgtaaacat caaatcatca ccgatatgag
    31201 tgaagaagcg atggtcgaaa acgatctcac cggaccgttt atgccgcacg gtattggcca
    31261 tccgctgggc ctgcaggtgc atgacgtcgc cggttttatg caggatgata gcggtacaca
    31321 cctcgcggca ccggcaaaat atccgtacct gcgctacacc cgtattctcc agccgggcat
    31381 ggtgttaacc atcgaaccgg gtatctactt cattgaatcg cttctggcac cgtggcgtga
    31441 agggcagttc agcaagcact tcaactggca gaaaattgaa gcactgaaac cgttcggcgg
    31501 cattcgtatc gaagacaacg tggtgatcca cgaaaacaac gtggaaaaca tgacccggga
    31561 tctgaaactg gcgtgatgga aagctggtta attcctgcgg caccggtcac ggtcgttgaa
    31621 gagatcaaaa agagccgctt cataacgatg ttggcgcata ccgatggcgt tgaggcggcg
    31681 aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg cccgccacca ttgcgtggcg
    31741 tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt tctctgacga cggggagccg
    31801 gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg gcagcggcgt cggggaaatt
    31861 accgctgtgg tagtgcgcta ctacggcggc atattgcttg gcaccggtgg gttagtgaaa
    31921 gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa cgacccaacg caagacgcca
    31981 ttaaccgaat atactttgca atgtgaatat agtcagttaa ccggcattga agcgttgctg
    32041 gggcagtgtg acggcaaaat tatcaacagt gattatcagg cattcgttct gctgcgggtg
    32101 gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc tggcggattt tagccgtggt
    32161 tcattgcaat tgttagcgat tgaagaataa tccccacttc gttttgcaga actaaggaag
    32221 cggcagagat gcattttcgc gccattaccc gaatcgttgg actactggtc atcttatttt
    32281 cagggaccat gattatccct gggctggtag cactcatcta ccgggatgga gcgggccgcg
    32341 cttttaccca gacctttttt gtcgccctcg ccattggctc tatgctgtgg tggccgaacc
    32401 gcaaagagaa gggcgaactg aaatcccgtg aggggtttct gatagtggtg ctgttttgga
    32461 ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc ggaaagcccg aacctcacga
    32521 ttaccgatgc gttttttgaa tctttctctg gcctgaccac caccggggcc actacgctgg
    32581 tggggctgga ttcgctccct catgccatcc tcttttatcg ccagatgctg caatggtttg
    32641 gcgggatggg gatcatcgtg ttagcggttg cgatactgcc tatcctcggc gtgggtggga
    32701 tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa
    32761 ttgcggaaac ggcgaaaact ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc
    32821 tggcgttgtg gtttgccgga atggatgcct ttgatgccat cggccatagc tttgcgacta
    32881 tcgctattgg cggcttctcg acacatgatg ccagtatcgg ttatttcgac agtccgacta
    32941 ttaacactat cattgctatc ttcctgctga tctccggctg taactacggt ctgcactttt
    33001 cactgttaag tgggcgtagt ctgaaggttt attggcgcga tccggaattt cgcatgttta
    33061 tcggcgtaca gtttacgctg gtggttattt gtacactcgt actgtggttt cataatgtct
    33121 acagttcggc gctgatgaca attaaccagg cgtttttcca ggtggtatcg atggcgacaa
    33181 ccgccgggtt tacgactgac agcattgccc gctggccgct ctttttgccg gtactgcttt
    33241 tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg cggtggcctg aaagtgatcc
    33301 gcatcctgct gctgtttaag caggggaacc gtgagctgaa acgactggtg catccgaacg
    33361 ccgtctatag cattaagctg gggaatcgcg cactgccgga acgtatcctc gaagccgttt
    33421 ggggattttt ctccgcctat gcattggtgt ttattgtcag tatgctggcg attatcgcca
    33481 cgggcgtgga tgatttttct gcctttgcct cagttgttgc gacattgaat aacctggggc
    33541 cagggcttgg cgtggttgct gataacttta ccagtatgaa cccggtggct aaatggatcc
    33601 tgattgccaa catgctgttt ggtcgtctcg aggtctttac attactggtg ctctttaccc
    33661 cgactttctg gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt tctcaacaag
    33721 ggacggacaa acgcgcgaga ttgcctccta cctggcttcg gaactgaaag aactggggat
    33781 ccagacggat gtcgccaatg tgcaccgcat tgaagaacca caatgggaaa actatgaccg
    33841 tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc aggaatttgt
    33901 caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc ttttattccg taaacctggt
    33961 ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc tacgcgcgga agtttctgat
    34021 gaactcgcaa tggcgtcccg atcgctgcgc ggtcattgcc ggggcgctgc gttacccacg
    34081 ttatcgctgg tacgaccgtt ttatgctcaa gctgattatg aagatgtcag gcggtgaaac
    34141 ggatacgcgc aaagaagttg tctataccga ttgggagcag gtggcgaatt tcgcccgaga
    34201 aatcgcccat ttaaccgaca aaccgacgct gaaataagca taaagaataa aaaatgcgcg
    34261 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    34321 ccactgacac ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaattc
    34381 cgacagagaa agcaaaaata aatgcttgac tctgtagcgg gaaagcgtat tatgaacacc
    34441 ctgcgccgct gagaaaaagc gaagcgg
//
LOCUS       DAJVRL010000034        33890 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVRL010000034 DAJVRL010000000
VERSION     DAJVRL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.034"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            14..382
                     /locus_tag="OQN02_001665"
     CDS             14..382
                     /locus_tag="OQN02_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1642501.1"
                     /translation="MMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSALELARNHL
                     GIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRVQYVLDEMLREI
                     RHQLAQSQQLRPEQAAESED"
     gene            complement(379..1515)
                     /locus_tag="OQN02_001666"
     CDS             complement(379..1515)
                     /locus_tag="OQN02_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1642502.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1521..1844)
                     /locus_tag="OQN02_001667"
     CDS             complement(1521..1844)
                     /locus_tag="OQN02_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1642503.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2027..2170
                     /locus_tag="OQN02_001668"
     CDS             2027..2170
                     /locus_tag="OQN02_001668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642504.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2389..3927)
                     /gene="aldB"
                     /locus_tag="OQN02_001669"
     CDS             complement(2389..3927)
                     /gene="aldB"
                     /locus_tag="OQN02_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCS1642505.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4092..5243)
                     /gene="yiaY"
                     /locus_tag="OQN02_001670"
     CDS             complement(4092..5243)
                     /gene="yiaY"
                     /locus_tag="OQN02_001670"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCS1642506.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5433..7277)
                     /gene="selB"
                     /locus_tag="OQN02_001671"
     CDS             complement(5433..7277)
                     /gene="selB"
                     /locus_tag="OQN02_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1642507.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7274..8665)
                     /gene="selA"
                     /locus_tag="OQN02_001672"
     CDS             complement(7274..8665)
                     /gene="selA"
                     /locus_tag="OQN02_001672"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1642508.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8763..9371)
                     /locus_tag="OQN02_001673"
     CDS             complement(8763..9371)
                     /locus_tag="OQN02_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1642509.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9442..10527)
                     /locus_tag="OQN02_001674"
     CDS             complement(9442..10527)
                     /locus_tag="OQN02_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1642510.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10530..10892)
                     /locus_tag="OQN02_001675"
     CDS             complement(10530..10892)
                     /locus_tag="OQN02_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1642511.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11429..13342
                     /gene="mtlA"
                     /locus_tag="OQN02_001676"
     CDS             11429..13342
                     /gene="mtlA"
                     /locus_tag="OQN02_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1642512.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13437..14585
                     /gene="mtlD"
                     /locus_tag="OQN02_001677"
     CDS             13437..14585
                     /gene="mtlD"
                     /locus_tag="OQN02_001677"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1642513.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14585..15172
                     /gene="mtlR"
                     /locus_tag="OQN02_001678"
     CDS             14585..15172
                     /gene="mtlR"
                     /locus_tag="OQN02_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1642514.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15184..15393)
                     /locus_tag="OQN02_001679"
     CDS             complement(15184..15393)
                     /locus_tag="OQN02_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642515.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15677..16039
                     /locus_tag="OQN02_001680"
     CDS             15677..16039
                     /locus_tag="OQN02_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1642516.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16583..17266
                     /locus_tag="OQN02_001681"
     CDS             16583..17266
                     /locus_tag="OQN02_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1642517.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17310..21675
                     /locus_tag="OQN02_001682"
                     /pseudo
     CDS             17310..21675
                     /locus_tag="OQN02_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22043..23698
                     /gene="lldP"
                     /locus_tag="OQN02_001683"
     CDS             22043..23698
                     /gene="lldP"
                     /locus_tag="OQN02_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1642518.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23698..24474
                     /gene="lldR"
                     /locus_tag="OQN02_001684"
     CDS             23698..24474
                     /gene="lldR"
                     /locus_tag="OQN02_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1642519.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24471..25661
                     /gene="lldD"
                     /locus_tag="OQN02_001685"
     CDS             24471..25661
                     /gene="lldD"
                     /locus_tag="OQN02_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1642520.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            25857..26330
                     /gene="trmL"
                     /locus_tag="OQN02_001686"
     CDS             25857..26330
                     /gene="trmL"
                     /locus_tag="OQN02_001686"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1642521.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26383..27204)
                     /gene="cysE"
                     /locus_tag="OQN02_001687"
     CDS             complement(26383..27204)
                     /gene="cysE"
                     /locus_tag="OQN02_001687"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1642522.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27284..28303)
                     /gene="gpsA"
                     /locus_tag="OQN02_001688"
     CDS             complement(27284..28303)
                     /gene="gpsA"
                     /locus_tag="OQN02_001688"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1642523.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28303..28770)
                     /gene="secB"
                     /locus_tag="OQN02_001689"
     CDS             complement(28303..28770)
                     /gene="secB"
                     /locus_tag="OQN02_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1642524.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(28833..29084)
                     /gene="grxC"
                     /locus_tag="OQN02_001690"
     CDS             complement(28833..29084)
                     /gene="grxC"
                     /locus_tag="OQN02_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1642525.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29226..29657)
                     /locus_tag="OQN02_001691"
     CDS             complement(29226..29657)
                     /locus_tag="OQN02_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1642526.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            29902..31446
                     /gene="gpmM"
                     /locus_tag="OQN02_001692"
                     /gene_synonym="pgmI"
     CDS             29902..31446
                     /gene="gpmM"
                     /locus_tag="OQN02_001692"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1642527.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31456..32739
                     /gene="envC"
                     /locus_tag="OQN02_001693"
     CDS             31456..32739
                     /gene="envC"
                     /locus_tag="OQN02_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1642528.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32743..33702
                     /locus_tag="OQN02_001694"
     CDS             32743..33702
                     /locus_tag="OQN02_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1642529.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33689..33890)
                     /locus_tag="OQN02_001695"
                     /pseudo
     CDS             complement(33689..33890)
                     /locus_tag="OQN02_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8418 a         8881 c         8653 g         7938 t
ORIGIN      
        1 tttgatcgac tcgatgatga tcgacgacgc gaatatctgg cgcttcaacg tcgccgggag
       61 taaagaggtg cgggattatc gcgtgaaggg aaattacgtt tgcgataaca cgcaatcggc
      121 gctggagctg gcacgaaatc acctggggat tgtgtttgcg ccagacaaaa gtgtgcagag
      181 cgatctgcaa gacggcacgc tggtgccctg ctttcagcac ccgtatgaat ggtggctgga
      241 tctggtggct atcttccgta aacgggaata cctgccctgg cgggtccagt atgtgctgga
      301 tgaaatgctg cgtgaaattc gccaccagct tgctcagtcg cagcaactgc ggcctgaaca
      361 agcggcggaa agcgaagatt aatgatcgag ataaaggtaa tgcacccagc tggtcatgcg
      421 taatagcact ttgcgcatca ccgagacatg gctgaaatga tcagagtaga ccgccacctg
      481 ggcgtaaata ccgtctggta aagcgctcaa atcagtgtgc tcatccagtt caatggtcgc
      541 gataacgcca tctgaacccg gcgctgtgtt taacgtctgt aaggtgccgg tcgactgata
      601 agctccgccg ggaacagcag gactaatggc tgccagcttg ccgctgaata ccttacctgg
      661 cagagcatta aacaccactt ccgcatcgtc gccaggggcc agtcgcagca aggagttctg
      721 acggaactgc gccacaattt gtcgtttctg atcgggtata aacaccatca ccggacgtag
      781 cggcagcgac gcggcatagg tacccggacg aatcagcacc tgggtcacat aaccatcgct
      841 cggcgcacgc actatcgtct gctcaaggtt atattttgct tccgcgagct gcgctttcag
      901 gctggcgatt tgagaatgtt cacccaacac caggctatcc agctggctct ggatctgttt
      961 ttgttccgcc gccgatgact ttacggaggc ttcctgcgcc agataatttt gccgcgccac
     1021 atcaatatcg cgttctgaaa acgggtttac tttcgcctgg ctgccgcgtg cgtaacgctg
     1081 atactcttta gcgaatttat cccgcgtggc ctttgcctgc tgagtattcg ccgccatctc
     1141 atctaactct gcgcccaacg cccgctgttt atgttctgcg gtaacgatat ccgccatcag
     1201 ccgatccacc cgcgcctgat aacgcgtcgg gtccagtcga aatagcacct cacctttttt
     1261 aatcagcgta tttttcttat ccgtcacttc gatcaccacg cctgtcacct gtgggacaac
     1321 aggaatagaa ataaccgctt tttgcgcctt gaaggtatac ggatggttat agttcattaa
     1381 cagaattaaa ccactgacaa taaatatgcc acccagggcc gctgtggaaa tggtccattt
     1441 atttacagga attttgaaga tcttaaacat tgcccatgca aaagccacat aggtcaaaat
     1501 aatcagtaaa tccataagta ttactcggca gaagaggatt tgatgtcggc gagctgtttc
     1561 tccagcccgg caatacgctg ttgtagttgc acaacggagg agtcatggat ttgcattccc
     1621 catccccgct ccgggcggta aagcgtggcc caaatccaca aaaatggcca gataacatgc
     1681 agcgtaaaaa gactcaccca accagcaaca tgaatggcgt cggcatgagg atgattacgt
     1741 tttttagcaa tcaggtaggg aatatcatgt aaaataataa tcccatagaa tatcaccaga
     1801 aatacaaaaa taagcactcc cagtgcaaaa tagtccagga acatatttcc ctcaaagaat
     1861 ataaaaaaga acaattaacg catattatga aatgccatga tgcagtgcaa tttccttatt
     1921 tatataacga catattgttt tttatatact ttctgaaatg tgctgtctgg tttttcaaca
     1981 ggacgaggtt tatcatattg aaatagtgga actattggcc aaactaatga ataactccag
     2041 taaaacatcc acagtacaga ttaagcgtat taaaccttca attatctacc gtttattgct
     2101 gattggcctc ggatcaccaa tggtgattta cggcctggtt cgcccgctca ccatcgaaac
     2161 gcgagattaa actcctgaca aaacacgccc cagaaacgca cttctgttgg cgtagtctgg
     2221 gttattgcgc acgtaggttt aaaacgtaaa ttgtaacaac gtgcgctttg tttatgccgg
     2281 atgcggcgtg tacgccttat ccggcctacg gtcaggttcc cgtaggcatg ataagacgcg
     2341 taagcgtcgc atcaggcaat gaatacccaa tgcgaccagc ttcttatatc agaacagccc
     2401 caacggttta tccgagtagc tcaccagcag gcacttggtt tgctggtaat gctccagcat
     2461 catcttgtgg gtttcgcgac cgatacctga ttgtttgtag ccaccaaacg cagcatgtgc
     2521 cgggtaagcg tgataacagt tggtccacac gcgcccagcc tgtatgccgc gccccatctt
     2581 ataggccaga ttaccgttgc ggctccagac gcccgcgccc aggccatatt gcgtatcgtt
     2641 cgccagctcc agcgcttctt ccatcgtttt gaaggtggtc accgccagca ccgggccaaa
     2701 aatctcctcc tggaacaccc gcatattgtt ctgaccaaac agaatcgtcg gttcgaggta
     2761 gtagccgtct ttcagttcac cttccagcag cttgcgccgc ccgcctgtga gcacgtcagc
     2821 gccctctttt ttaccgatat caatgtagtt gaggatggtt tccagttgcc cgtgagaaac
     2881 ctgcgcgccc atttgcgtca cgctgtcgag cgggttaccg ctgcgaatgc tttcgacacg
     2941 gcggatggcg cgttccataa agcgttcgta gattgactcc tgcactaagg cacgactcgg
     3001 acaggtgcaa acttcgccct ggttaaaggc aaacagtgca aagccttcca gcgctttatc
     3061 gaaaaaggca tcttcttcat ccatcacatc agcaaagaag atatttggcg acttaccgcc
     3121 caactccagc gtcaccggaa taatgttttg cgttgcgtat tgcataattt gttggcccac
     3181 ttccgttgag ccggtaaacg ccactttggc gatgcgtttc gaggtcgcca gatattcgcc
     3241 aatttcccca cctgcgccat tgaccacgtt caccacgccc ggcggcagta aatcaccgac
     3301 aatttccatt agcagcagta cagaaagcgg ggtaagacgt gcgggtttca gcaccacaca
     3361 gttgcccgcc gccagcgcgg gagccatttt ccagctcgcc atcagcagcg ggaagttcca
     3421 cgggataatc tgccccacca cgcctaacgg ttcatggaaa tgataggcca cggtttcgct
     3481 atcaacttca ctgatcccac cttcctgcgc ccgaatacac gaggcgaaat agcggaaatg
     3541 gtcaatcgcc agcggtacat ccgcagcact ggtttcgcga atgggtttgc cgttatccca
     3601 ggtttcagct gtcgctaaca gctcgaggtt ttgttccatt cgatcggcaa tcttaaacag
     3661 aatcgccgcg cgatcctgca ccgaggtgtg cgcccattta tctttcactt tgtgcgcagc
     3721 atccagcgcc agatcgatgt ctcgtttgcc cgaagacgcc acttcgcaca gcagctgccc
     3781 ggtcaccggc gtcagattct ggtaatactc accatcggca ggggctaccc attcaccgcc
     3841 aataaagttg tcgtagcggg cttttaactt gatggggaaa ccatactcgc cgggcttaat
     3901 ctgtgctgaa gggggattat tggtcatgat cgtctccttg cggtgtgagg tataacaagg
     3961 gtagacgtga ctggcgaaat cttcgccagt cggtaacagc tttacgacag ctatcacgaa
     4021 tttacgggca aggattacag agatagttga aaaagcggct gacaatttgc cagccgttgt
     4081 tggaatgatg attacattgc ggcgcgataa atcaccacaa tttcttcgtg agttgcctgg
     4141 atcgggttag taaagccaca ggcatctttc agggcattag tcgccagaac cgcgaaatct
     4201 tcttctttca cgttcaggtc gcgtaggcct gccgggatat ccactttctt cgccagttca
     4261 cggatggcgt taatgcaggc ttcagcacct tccgcgtcgt ttttacctgt cacgttcacg
     4321 cccattgcag cggcacagtc acgcagacgt gcagcggcga ctttgctgtt gaatacctga
     4381 acatgcggca gcagaacggc gttacataca ccgtgtggca ggttgtagaa accgcccagc
     4441 tggtgcgcca tcgcatgaac ataacccaga gaagcattat tgaacgccat accggcaagg
     4501 aactgggcat aagccattgc ttcacgcgct ttcgcattac tgccatcttc aacggctaac
     4561 ggcaggtttt cggcaatcat ggtcacggct ttcagtgcac aagcgtcagt gatcggcgtg
     4621 gcggcaatag aaacatacgc ttcgatagcg tgcgttaagg catccatacc cgttgcggcg
     4681 gtcagtgact tcggcatacc aatcatcaga gaggagtcat tgacagaaag cagcggagtc
     4741 acgtgcttat caacaatcgc cattttgtta tgacgcgctt cgtcagtgat gatgcagaaa
     4801 cgggtcattt cagaggccgt acccgccgtg gtattgatgg cgatcatcgg cagctgcggt
     4861 tttgcagagc ggtcaacgcc ttcgtaatcg cgaatatcgc cgccattggc tgccaccagc
     4921 gcaatacctt ttgcgcagtc gtgtggagaa ccaccgccta aggagatcac gctatcgcaa
     4981 ttattttctt taagtaattt caaacctgcg gcgacgtttt ccgtggtggg gttaggttgg
     5041 gtgccatcat aaataacgct aaaaatattg cgttcttcca gtgctttttg cacatcaccc
     5101 gccataccta atttcgttaa catattgtca gtgacaatta aggtacgggt aaatccataa
     5161 tctgccatca tattcattgc atcagtcaat gaatcagcgc cgatgacatt cacagaagga
     5221 ataaagaaag ttgaagatgc cataacacac tcctgaaagt gtaaaaaagg gtgggcagct
     5281 tactgaggat tttcattcga aaacatgatc attattatta attatcaaaa ttaatctaat
     5341 aaaaagcaca ttgtattcat taaacaatgt gctttattta aaatcataag aaatttgtat
     5401 taatttattt taaaaattaa ttaatcattt ccttattttt ccggaaataa taatgcgtcg
     5461 cgcaataaat gatcatttcc acgacgacgc gtaaagccaa tgcggtcaaa atattccaga
     5521 atctgaattg ccagctttcg gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt
     5581 gaaccacact cctgatcgag atcgcggatc atattggcaa attcgacaat ccgatcgtta
     5641 cggtaataac gatctttgac gatcgcggta attattcctt gctgcgccgc ctggcgtagc
     5701 gtcaggcgca ttgcctgctc gtcggtaccc gtctcttttg ccagatcacg cacccaccac
     5761 ggctcgtcac caaacagcgg ctctgctttt tgccagatgg cctgctgctc ttcgctgaag
     5821 cccgctttgt gatccggcag atgcagccaa ccgtgatgac tgaggatgtc gccgctttcg
     5881 cgcatctttt caatcagcaa cagtactagc gcttcatctt ccattggcaa cgccatacgt
     5941 cgcagacgtt cgcgcccagg cccaggttca tcgcgatgtt gctcatgata agtcgctaat
     6001 gtgtcgagaa ttttccgttg ccagcgagcg gcaaccggcg cattcaacaa gctataacca
     6061 gcctgaatat agccaggctg ttgcagcagt tcgcgcatcc cttcgccgtt gagctggcgc
     6121 gcccaggcga aatcaggaag attaaccgcg ccgcgttgca ggtgaacggc taacgcttcg
     6181 ctatcattct gtgccctggc gagggaagcc agccattgca gatactctgg tttacgttta
     6241 ccgcgacgcg gcgggttaag catcacgacg cgcgctccgg ccagcgtgtt gcgggcagag
     6301 atatcgcgca ataccaggcg gtcgttatct gccagccata acggggtgtc gaagaccagt
     6361 tcagcaaggt tatcttccag cagtgaaacg cgtcccgtga cgtggctggc ggcgtggtga
     6421 atatgcagcg gctgccactg ggtcagcggt gcatgggttt gaagctcgac aatcacccgt
     6481 gtgaacggct ctggcggcac atcggcaagc agccagtcgc cacggttaat ctgctctttt
     6541 tccgcatcac ccgcgatgtc aagcgcgata cgctgcccgg cattggcggt ttctgttggc
     6601 tggttttgcg catgcagcgc acgtacacgc atcggtttat ttacaccagt cagccagagt
     6661 gaatcgccta ccttcacttc cccgcttaac gccgtaccgg tgacgaccag cccggcacct
     6721 tttacggtaa atgcgcggtc aatcgcgagg cggaaactat gttggctggc gtgctcgcgt
     6781 tccggcaact gaagcagatg ctcgcgcagg gcatcaattc cccgaccttc ggttgctgcg
     6841 gtgataaaca gttttgcctc agcaaaaccg tattcccgca gaacctcctt tacctggcgt
     6901 tcaacctcat caacacgcgc ttcgtccacg cgatcggctt tggtcagcgc cactgtcagc
     6961 atcgggttac cggtcagctg caaaatcgcc agatgctcac gggtctgtgc catcacgccg
     7021 tcatcgcacg ccaccaccaa cagcgcgtga tcgataccac caacgcccgc cagcatgttg
     7081 gaaagaaact tttcatgacc gggaacgtcg ataaaaccag gcacgcgacc atccggctgc
     7141 ggccagtagg catagccgag atcgatggtc atgccgcgct ttttttcttc cggcagacgg
     7201 tcagcattta cgccagtaat cgcctgcaat aaggttgttt tgccgtggtc aacgtgtccg
     7261 gcagtcgcaa taatcatttc aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc
     7321 gtaaatccag ccacaatcgt ccgtcataaa tacgaccaat caccggcact ggcaattcac
     7381 gccagcgggc ggctaatgac tcaaggtggc taccgcgtcc atcatggggt gtaaacgtta
     7441 atgccgcgct cggcaggcga tcaaccggca gcgaaccact gccaatctgc gaaagacatg
     7501 gcataacctg taccgcaaac tccgcgccgt aatgtgcgac aaggggagcc tgtaaacgtt
     7561 gtgcctggat ttgaatgacc tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt
     7621 cactcagagc ttcagggtgt aaataaagac gcaacgtggc ttccagcgcc gcgagggtca
     7681 ttttatccgc gcgtaatgca cgcttcagcg ggtggctttg caggcgggcg atcatctctt
     7741 ttttaccaac gataatcccg gcctgcggcc cgcctaacaa cttgtcgccg gagaagctca
     7801 ccagactgac gcccgccgca atcaactcct gcggcattgg ctctttcggc aaaccgtact
     7861 ggctaagatc gaccagcgag ccactgccta aatcagtcac cactggaaca tccagctctt
     7921 tgccgagcgc caccagttca gcttcgtcta tcgctttggt gaacccctga atgctgtagt
     7981 tactggtatg tactttcatc aacagtgcgg tattttcatt caccgcctga cgataatcat
     8041 tcgcgtgcgt gcggttggtg gtccctactt cgtgtagggt gcagcctgcc tgacgcataa
     8101 catcgggaat acgaaacgcg ccgccaatct ccaccagttc gccgcgagat accaccacct
     8161 ctttcccgct ggcagtggcc gccaacatca ataacaccgc cgccgcattg ttattgacga
     8221 tacaggcatc ttccgccccc gtaatacggc acagcagctg cgccagcgcc cgatcgcgat
     8281 gtccgcgtcc ggcgtcgtcc agatcgtact cgagggtcac tggcgaacgc atagcctgcg
     8341 caacggcttc caccgcggct tccgcctgta aagctcgccc aaggttggta tgcagcacgg
     8401 ttcccgtcag gttgatcacc ggacgcagcg cgctctgcgc ctctttcgtc aaccgggcat
     8461 cgacttcttg cgcccagttt tcacaccacg caggcagcgt ctggctgcca cgaatcactt
     8521 ctcgcgcttc gtcgagcatc tgacgcaaca attccaccac gcgggtgtga ccataagtat
     8581 cacgcaaaga aaggaaggag ctatcgcgca ataagcgatc aatagccgga agttgactat
     8641 agagggaacg cgtttcggtt gtcataggaa acctggctga tcaaggccct ctcacacgga
     8701 gaagggcgtt taacataacc acggattgta acgtgagatg ggtcaggagg acatatcgcg
     8761 catcaagcct ttggcggttc ggtgcgagca aaactttcgc ggctaaacag gttttcgacc
     8821 agtttgacta agtgtgggcg gtcgacacac caacctggcg caacgcggcg gaaattgaga
     8881 tatccgacag cacaggcaat ggcgatagtc gccagattga ccgtatcggt tttgagtgtg
     8941 ccatcgacca gatatccttc cagcacatcc agactgcggt tgattttctc ccgctggcgt
     9001 aacaattcat cttcagactg ctgcgccgcc ggacgcgcct gttcacgcac cgataccagc
     9061 ccggcatcca taatgccatc cgccagcgcc tcaattttgc gcacccgcaa cgactccagc
     9121 ggatcgcgcg gcaacatcgc cggagcgaca ttcattaact caatatattc agcgatgatc
     9181 ggcgaatcaa accagcattc gccctcttcg gtcaccagca ccggcacttt ccctaacggg
     9241 ttaaattgcg ccacgccgtt atccgcgtta tagggcagtt cattaataaa ttcgaaagtt
     9301 atgccctttt ctaacaacag aatagaaagt ttgcgtacaa acgggctggt gtagctaccg
     9361 acgagtttca tgccgagtcc tttgtgcgag gaaaaatatc agtatggctc aggctcacgc
     9421 aaaacacagg tgatataagt ctcaatgatc caaataaaga taatgcatcc aactggtcat
     9481 tcttagcagc actttccgca tcaccgaaac atggctgaaa tggtcggagt aaaccgccac
     9541 ctgggcgtag atgccgtcgg gtaaggcatc gatatcatcg ttagggtcca gttcaatggt
     9601 tcccagcaca ccgtccgtgc cgggaacgac cgttaatgat tgcaataccc cctgcgcctg
     9661 ataagaaccg cctggcacga caggtaaaat actagtcaat ttgccgtgga acacctgccc
     9721 aggtagcgcg ttaaacacca cttccgcatc atcgccaggt ttcagacgta acagcgagtt
     9781 ttgccgaaat tgggcgacaa tttgccgttt ttgctcgggg atgaagacca tcaccggacg
     9841 cagcggcaag gcagctgcgt atgtacctgg gcggatcagt acctgagtga cgtagccatt
     9901 gcttggtgcg cgaatgacag tctgctcaag attatatttt gcttcagtaa gttgcgctct
     9961 taagctcaca atctgcgatt gctcgccgtt gaccatactg tcgagctgac tctggatctg
    10021 cgcctgctcc gccaccgagc ctttcactaa cgcatcctgc gcgaggaaat tttgccgcgc
    10081 atcgtcgatg tcacgttccg agaacgaatt caccgccgcc tggctgcctt tcaggtaacg
    10141 ttgataattt ttaaacagac ggtcgcgctc cgctgaaacc tgggtggtgt tggcctgcgc
    10201 ttcagtgagc tgcgcacgca gcgtctttat attatgcgtc gccgtcatca ggtcagcctg
    10261 aagtctgtca actcgcgcct ggtaacgaac cgggtcgagc ttaaaaagca cctcgccctt
    10321 ttgaataagc tgattattct tgtcagtgac ttcagtaaca attcccgtca cctgtggcgt
    10381 gatagggatc gctatcactg ccttttgcgc ggtaaaagtg taagggtggt tgtagttcat
    10441 caacaaaatc aaaccactca ccagaaacac gcctcccagc gccgccgtcg ccagcgccac
    10501 gtaagttaaa acaatcaata gatccataat tagatctccg ggaacgtgga atagtcagtc
    10561 tttttctcgg cggcgagttg atgctccagc cgggaaattc ggtcagaaag tgcggcgatt
    10621 tccggttcag tcgctttctc ctgcgacgca acatgtgact gcatacccca gccacgctcc
    10681 ggttgataga gcgtcgccca gatccacaga aacggccaga taacatgcag agtaaacagg
    10741 ctcacccagc ccgccgtatg aatagcgtcg gcatgggggt gattgcgctt tttggcaatc
    10801 agataaggga tgtcatgtat cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc
    10861 agcactccca acgcgaaata gtttagaaac atatctgcct cggattcacg tttatcagtg
    10921 ttgttttttg gctggcagcc agaagggagt caggctgata ttttgacaat aatccgggtt
    10981 cgcgattctc gccataacac caaagaataa tttttagagg tgatgagttg cttagttaca
    11041 taacgattgt atgacgaagg cataacatgc tgtagatcac atcaggtgaa cgccgtaaga
    11101 aaatatcttg tgattcagat cacaaagatt caacaaacca tcaaaacaaa aatgtgacat
    11161 tactcacatt taaatgccat ttttagcgaa aatcaccgcc ttgtttcttt tttatacagg
    11221 cgttttgtga tgagcgtcac gtcaattacc tctctacccc ctatatttat gtgactgata
    11281 tcacacaaaa gcccgtcgac tggacagtta accgattcag tgccagattt cgcagtatct
    11341 acaaggtccg gctacctctg ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc
    11401 gacagcaaac ataagaaggg gtgtttttat gtcatccgat attaagatca aagtgcaaag
    11461 ctttggtcgt ttcctcagca acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg
    11521 tatcatcacc gcgttattta ttccaacagg gtggttaccg aacgagacgc tggcgaagct
    11581 ggtcgggccg atgatcactt atctcctacc gctgctgatc ggttataccg gtggtaagct
    11641 ggtaggcggc aaacgtggtg gcgtagtcgg tgccattacc accatgggcg ttatcgtcgg
    11701 cgcagacatg ccgatgttcc tcggttctat gattgcaggt ccgctgggcg gctggtgcat
    11761 taagcacttc gaccgctggg tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa
    11821 taacttctcc gcaggcatca tcgggatgat cctcgctatt ctggcattcc tcggcattgg
    11881 cccgattgtt gaagccctgt ccaaaatgct ggctgcgggc gttaacttca tggttgtcca
    11941 tgacatgctg ccgctggcgt ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa
    12001 cgccattaac cacggtatct tctcgccgct gggtattcag cagtcccatg aactgggtaa
    12061 atcaatcttc ttcctgattg aagctaaccc aggtccaggt atgggcgtgc tgctggcgta
    12121 catgttcttt ggtcgtggta gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt
    12181 cctgggtggt atccacgaaa tctacttccc gtatgtgctg atgaatccgc gtctgatcct
    12241 cgcagtcatc ctcggcggta tgactggcgt gttcacgctg actatcctgg gcggtggtct
    12301 ggtttctccg gcatctccgg gttctatcct tgctgtactg gcgatgacac caaaaggtgc
    12361 ttatttcgct aacatcgcgg gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc
    12421 tattttgctg aaaaccagca aagtgaaaga agaagatgat attgaagcag caactcgtcg
    12481 tatgcaggac atgaaagctg agtctaaagg cacatctccg ctatctgcgg gcgatgtgac
    12541 taacgacctg agccacgtac gtaaaatcat cgttgcctgt gacgccggta tgggttcaag
    12601 tgcgatgggc gcaggcgtgc tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc
    12661 ggtcactaac agcgcgatca acaacctgcc gccagatgtg gacctcgtca tcactcaccg
    12721 tgacctgacc gaacgcgcta tgcgccaggt tccgcaggca cagcatattt cgctgaccaa
    12781 cttcctcgac agcggcctgt acaccagcct aaccgaacgt ctcgttgctg cccagcgcca
    12841 tactgaaaac gaagtgaaag tgaaagacag tctgaaagac agctttgacg attccagtgc
    12901 taacctgttc aaactgggcg cggagaacat cttcctcggt cgcaaagcag caaccaaaga
    12961 agaagcgatt cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg agccggaata
    13021 cgttcaggcg atgctggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc
    13081 ggtgccacac ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg
    13141 ccagtacccg gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat
    13201 tggtattgca gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact
    13261 ggatgatgag tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga
    13321 actgctggca ggtcgtaagt aatccaatcc taccctctcc tcacggagag ggtttgggtg
    13381 agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga
    13441 aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag
    13501 acgcgggtat ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg
    13561 cccgtcatag ctatcaggtt catgtggtcg gtgaaaccga gcaggtagat accgtttccg
    13621 gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga tctgattgct caggtcgatt
    13681 tagtcactac cgccgttggc ccggttgtgc tggaacgtat tgctccggct atcgccaaag
    13741 ggctggtgaa acgcaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa
    13801 acatggtacg cggcaccacg cagctgaaag gtcatgtgat gaacgccctg ccggaagacg
    13861 ccaaagcgtg ggtagaagag cacgttggct ttgtcgattc cgccgttgac cgcatcgtac
    13921 cgccttcggc ttcggcaact aacgatccgc tggaagtgac ggtagaaact ttcagcgaat
    13981 ggattgtcga taaaacgcag ttcaaaggcg cactgccgaa catcccaggc atggagttaa
    14041 ccgacaacct gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta
    14101 taaccgcgta cctcggaaaa ctggccggtc atcagaccat tcgtgacgct attctcgacg
    14161 agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc
    14221 gctacggctt tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg
    14281 agaacccgta tctgaaagat gatgtagagc gcgtaggccg tcagccgctg cgtaaactga
    14341 gtgctggcga ccgtctgatc aagccactgc tcggtacgct ggaatacagc cttccgcaca
    14401 agaatctgat tcaggggatt gctggtgcaa tgcacttccg cagtgaagat gatccgcagg
    14461 ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt
    14521 ccggtcttga tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc
    14581 aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca
    14641 ggcaacaatg gaacaaaccc aggccgttga aaaccgtgtg cttgagcgtc tgaatgctgg
    14701 caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct
    14761 tctggtgctt caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt
    14821 actcgacggc gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt
    14881 gggcgtcatt aaccgccagg aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga
    14941 gctaaatcac gacggcaacg agtacgcctt taccgacgac gaaatccttg gaccctttgg
    15001 tgaactgcac tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag
    15061 tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc
    15121 actgactgag ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc
    15181 tgcttactgc ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg
    15241 tagatataac ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag
    15301 ttttttcaga tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat
    15361 aagaagcgga acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc
    15421 gggaatgcgc ggcctccaat tttagtttag gttcccggta aagatgacat taaggaaaac
    15481 gtgctgaatc ctcaataatc atccgctgct gtttattatc tttttctata gatttcttaa
    15541 ttaatcaacg aattgcgtag tttttgagca tgttaatgat tacgttgcag aactattaca
    15601 atgtcatcag ttgtgaacaa agcacctggt cgcgcatact aggggctata aatttatctt
    15661 tatcagaagc catcacatga aagaagtcga aaaaaacgaa atcaagcgtc tcagcgatcg
    15721 cctggacgcc atccgccacc agcaggccga tctgtcgctg gttgaagccg cagacaaata
    15781 tgccgagctg gaaaaagaga aagccacgct ggaagcagaa attgctcgcc tgcgtgaggt
    15841 tcatagccaa aaactgagta aagaagcaca gaagctgatg aagatgccgt tccagcgcgc
    15901 aattaccaaa aaagagcagg ctgatatggg caagctgaag aaaagtgttc gcggactggt
    15961 ggttgtgcac ccaatgaccg cgctggggcg cgaaatgggc ctggaagaga tgactggatt
    16021 ttctaagact acgttttaag cccctcagac gactggttcg atcgtatcaa cccgcatctg
    16081 gcttgcctta gctggtcaat gcggggactc tcctgtctac tcgcctaatt accaaacaat
    16141 tcactccctt tacttcgatt taccgctggt tgatattctg aaaatgcatt tttaccgaaa
    16201 tagccatcta taaacaataa aaatatatag aagatatcat ttgttaatta acaagcattt
    16261 acgtctcttt ttttcggatt taattgtgaa attttaatca tcatattcac aaaatcaatt
    16321 atctgtgaaa tttttcccag acatggtttc tttcatattt gacatttatt aataatcatc
    16381 attagaatcc acgaaaaata atatattcat acaaacacag acatcttttg aaacgaatga
    16441 tacattttta tgatgaatgc ttaaaacaat atttaatcga cacacatcac tgtcattaat
    16501 gtaaagcaac atagctcacc aataatacaa tggacaactc taccggcatt gatcgctggt
    16561 cggaggaaat aaggattttt acatgaactt gaagaagatt tttttcagtg ctgtcactgt
    16621 ttcagttctc tgcgcattaa cgggatgtga ctacattgaa gaaggcaagc cagaaagtag
    16681 tttactgaaa caacaagaag aacataataa taaaattgtg ctacttgaaa aacaacaagc
    16741 acagctaaag agtcaactcg aaactattca aaaacaacaa actggcataa taaacagtac
    16801 aaagacttta actcacgtaa ttaaatccgt taaagaccaa caaaatactt ttatttttac
    16861 cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aacggttcgg tggctttagc
    16921 gggtcgagtg ttatccattg acgccacaga gaatggtagt gttattcata tctcactggt
    16981 caacttatta agtacaccta tctcaaacat tggttttaat gcgacatggg gtggtgaaaa
    17041 acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca catcaatgaa
    17101 atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc tgaagggtgt
    17161 atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata ttcagttcga
    17221 caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc agttagtacg
    17281 cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt atctggaacc
    17341 ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc aagaaatctg
    17401 ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg
    17461 cattggcgaa tgccgggaat gacaacggtc agggtgttga ttacggtagt ggatcagctg
    17521 gcgacggctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg
    17581 gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc tctgccatcg
    17641 ggtcaaaaac ccatgcgatt ggtggtgcat caatggcctt tggggttagt gcaatatcag
    17701 aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa tactcaatgg
    17761 ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg gactcttcca
    17821 aagcggaagg agcaaacgcc attgccctgg ggcgtagcag tgtagctagc ggtactgaca
    17881 gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa cgctattgcg ataggtgctg
    17941 agaccgaagc cgctgaaaat gcaactgcta ttggcaataa tgcgaaggca aaagggacta
    18001 atagcatggc aatggggttc ggaagccttg ccgataaagt caatactatc gcattaggaa
    18061 atagcagcca ggctctggca gataatgcaa tcgccatagg ccagggcaac aaagctgatg
    18121 gcgtggatgc catcgctctg ggtaatggta gccagtcgag aggcttaaac accattgcct
    18181 taggcacagc cagtaatgca actggtgata agagtcttgc gcttggtagt aatagcagtg
    18241 ccaacggtat taactctgtc gcgctgggcg cagattccat tgcggattta gacaataccg
    18301 tctctgtcgg caatagttca ttaaaacgca agatcgttaa tgtgaaaaat ggcgcgatca
    18361 agtctgacag ttacgatgcc attaatggtt cacagcttta tgccattagc gactcggtag
    18421 caaaaaaggc ttggaggagg ggctgcagta gatgttgatg acggtactgt tacagcacca
    18481 acctacaatt taaaaaatgg tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat
    18541 gaaaacaccc tgcaatggga ccaaaccaaa ggcaaataca gcgctgctca tggtactagt
    18601 agcccaactg ccagcgtaat caccgatgtt gcggatggca cgatttcagc ctccagtaag
    18661 gatgcggtta acggttccca actgaaagct accaatgacg atgtcgaagc caacaccacc
    18721 aatatcacca acctgacgga ttccgttggt gaccttcagg ctgatgccct gctctggaac
    18781 gaaactaaaa aggcattcag tgcagctcac ggccaggata ccaccagcaa aatcaccaac
    18841 gttaaagatg ccgacctgac ggctgacagc actgatgctg ttaacggctc tcagctgaaa
    18901 accaccaacg atgctgtggc gacgaatacc accaatatcg ccaataacac ttccaatatt
    18961 gccactaaca ccaccaacat ctctaacctg actgagacgg tgactaatct tggtgaggat
    19021 gcgctgaaat gggataagga caatggtgta ttcacggcag ctcatggcac cgagaccacc
    19081 agcaaaataa ccaacgttaa agatggcgac ctgacgactg gcagcaccga tgccgttaac
    19141 ggctctcagc tgaaaaccac caacgatgcc gtggcgacga ataccaccaa tatcgccact
    19201 aacaccacca acatctctaa tctgactgag acggtgacta atcttggtga ggatgcgctg
    19261 aaatgggata aggacaatgg tgtcttcact gcagctcatg gcaacaatac cgccagcaaa
    19321 atcaccaata tcctggacgg cacagtcact gcaaccagtt ccgatgccat taacggtagc
    19381 cagctttatg acttaagcag caatatcgcc acctacttcg gcggcaatgc ttctgtgaat
    19441 actgacggtg tgtttaccgg tccaacctac aaaatcggtg aaacaaatta ttataacgtc
    19501 ggcgatgcac tggctgcgat taactcctca tttagcacgt ctctcggcga tgctctgctt
    19561 tgggatgcca ccgcaggtaa attcagtgcc aaacacggta ctaatggtga cgcaagcgtg
    19621 atcactgatg tcgcagatgg tgaaatttca gactccagtt ctgacgcagt aaacggctca
    19681 caactccacg gcgtgagcag ttatgttgtt gatgcgctgg ggggtggtgc cgaagtcaat
    19741 gcagacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
    19801 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc
    19861 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
    19921 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
    19981 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
    20041 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
    20101 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
    20161 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
    20221 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
    20281 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
    20341 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
    20401 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
    20461 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
    20521 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
    20581 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
    20641 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
    20701 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
    20761 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
    20821 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
    20881 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
    20941 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
    21001 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
    21061 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
    21121 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
    21181 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc
    21241 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
    21301 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
    21361 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
    21421 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
    21481 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
    21541 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
    21601 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
    21661 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
    21721 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
    21781 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
    21841 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
    21901 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
    21961 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
    22021 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
    22081 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
    22141 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
    22201 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcagtgg tttatggctt
    22261 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
    22321 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
    22381 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
    22441 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
    22501 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
    22561 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
    22621 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
    22681 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
    22741 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
    22801 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
    22861 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
    22921 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
    22981 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
    23041 gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt
    23101 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
    23161 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
    23221 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
    23281 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
    23341 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
    23401 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
    23461 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
    23521 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
    23581 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
    23641 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
    23701 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
    23761 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
    23821 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
    23881 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
    23941 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
    24001 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
    24061 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
    24121 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
    24181 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
    24241 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
    24301 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
    24361 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
    24421 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
    24481 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
    24541 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
    24601 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
    24661 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
    24721 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
    24781 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
    24841 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
    24901 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
    24961 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
    25021 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
    25081 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
    25141 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
    25201 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
    25261 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
    25321 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
    25381 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
    25441 acaccatact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
    25501 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
    25561 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
    25621 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
    25681 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
    25741 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
    25801 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
    25861 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
    25921 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca
    25981 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    26041 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    26101 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    26161 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    26221 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    26281 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    26341 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    26401 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    26461 gtctggttta ccgacaatac gaaccggaac gccagcggcg gttgtatgcg gcggcaccgg
    26521 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    26581 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    26641 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    26701 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    26761 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    26821 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    26881 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    26941 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    27001 ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    27061 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    27121 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    27181 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    27241 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct
    27301 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    27361 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    27421 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    27481 tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt
    27541 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    27601 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    27661 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    27721 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    27781 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    27841 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    27901 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    27961 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    28021 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    28081 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    28141 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    28201 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    28261 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    28321 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    28381 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    28441 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    28501 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    28561 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    28621 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    28681 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    28741 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    28801 atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca
    28861 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    28921 tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    28981 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    29041 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    29101 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    29161 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    29221 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    29281 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    29341 tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca
    29401 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    29461 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    29521 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    29581 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    29641 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    29701 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    29761 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    29821 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    29881 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    29941 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    30001 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    30061 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    30121 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    30181 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    30241 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    30301 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    30361 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    30421 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    30481 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    30541 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    30601 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    30661 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    30721 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    30781 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    30841 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    30901 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    30961 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    31021 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    31081 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    31141 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    31201 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    31261 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    31321 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    31381 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    31441 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    31501 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    31561 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    31621 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    31681 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    31741 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    31801 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    31861 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    31921 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    31981 caaacgcgtg aagaagtcac catgcagcgt gctgaactgg aagagaaaca aagcgagcaa
    32041 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    32101 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    32161 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgcgga agccgcggcg
    32221 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    32281 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    32341 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    32401 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    32461 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    32521 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    32581 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    32641 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    32701 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    32761 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    32821 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    32881 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    32941 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    33001 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    33061 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    33121 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    33181 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    33241 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    33301 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    33361 attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    33421 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    33481 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    33541 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    33601 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    33661 aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc
    33721 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    33781 ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta
    33841 aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg
//
LOCUS       DAJVRL010000035        33543 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVRL010000035 DAJVRL010000000
VERSION     DAJVRL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.035"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OQN02_001696"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OQN02_001696"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1642530.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OQN02_001697"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OQN02_001697"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1642531.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OQN02_001698"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OQN02_001698"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1642532.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OQN02_001699"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OQN02_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1642533.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OQN02_001700"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OQN02_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1642534.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OQN02_001701"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OQN02_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS1642535.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OQN02_001702"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OQN02_001702"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1642536.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OQN02_001703"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OQN02_001703"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1642537.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OQN02_001704"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OQN02_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1642538.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OQN02_001705"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OQN02_001705"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1642539.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OQN02_001706"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OQN02_001706"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1642540.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OQN02_001707"
     CDS             8212..9075
                     /locus_tag="OQN02_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1642541.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OQN02_001708"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OQN02_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1642542.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OQN02_001709"
     CDS             10411..11028
                     /locus_tag="OQN02_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1642543.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OQN02_001710"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OQN02_001710"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1642544.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OQN02_001711"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OQN02_001711"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1642545.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OQN02_001712"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OQN02_001712"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1642546.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OQN02_001713"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OQN02_001713"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1642547.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OQN02_001714"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OQN02_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1642548.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OQN02_001715"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OQN02_001715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1642549.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OQN02_001716"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OQN02_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1642550.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OQN02_001717"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OQN02_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1642551.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OQN02_001718"
     CDS             21859..23568
                     /locus_tag="OQN02_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1642552.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OQN02_001719"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OQN02_001719"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1642553.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OQN02_001720"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OQN02_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OQN02_001721"
     tRNA            27625..27719
                     /locus_tag="OQN02_001721"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28020..29204
                     /locus_tag="OQN02_001722"
     CDS             28020..29204
                     /locus_tag="OQN02_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1642554.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29616..30659
                     /locus_tag="OQN02_001723"
     CDS             29616..30659
                     /locus_tag="OQN02_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1642555.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..31602
                     /locus_tag="OQN02_001724"
                     /pseudo
     CDS             31174..31602
                     /locus_tag="OQN02_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31632..32783)
                     /locus_tag="OQN02_001725"
                     /pseudo
     CDS             complement(31632..32783)
                     /locus_tag="OQN02_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32848..33502
                     /locus_tag="OQN02_001726"
                     /pseudo
     CDS             32848..33502
                     /locus_tag="OQN02_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8403 a         8816 c         8267 g         8057 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcatgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaaagccg gaggatgtca
    28021 tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caagggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaaaata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat
    31441 attgttacac cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg
    31501 ccagcgacct ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt
    31561 ggtgagattg ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg
    31621 tggacacggc cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag
    31681 gcactgtgac gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc
    31741 attatttccc ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag
    31801 aagctttcca cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg
    31861 ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta
    31921 tgaacaatga cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag
    31981 gccagttgtg ctgtcattcg cggtgacatc gaccagccaa tgacggagcg tgaccacagg
    32041 tcgatgacta ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc
    32101 gcccacttct ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac
    32161 gggcaggcca tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag
    32221 cccctgacga cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc
    32281 aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc
    32341 gcaggtgagc cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata
    32401 ccagccgctg cgggcaactc gaagtacacg acacatcgct ttgatgctga actctgcccg
    32461 atgattttcg ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt
    32521 tggaggatgg acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca
    32581 gcggccagtt cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag
    32641 gcataaagct gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt
    32701 tcagcgagtt tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg
    32761 ctgattgata ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct
    32821 gtgtccacta ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat
    32881 atatcatctg taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt
    32941 gaatattata actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg
    33001 ttattatcac caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc
    33061 tttgtgacca aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta
    33121 ttttgctaca tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt
    33181 agttactcaa ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca
    33241 aagattgaaa aattatccaa cctatggttt ttattaccca atagccaatt ttatcgcatt
    33301 acaaataaat ggttcaaaaa caggatttgc cgcatctatt acaggtttta ctcaaacaat
    33361 aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc gacaacggtg ttggtcccgg
    33421 aactacttta ggtcttggtt cactaataac ttcattactg tcaatagccg taatcgccat
    33481 tgcaggaaat aataaaaagt aagtgtactc tgactgcgta tgtatctact tgaaccgccc
    33541 cgg
//
LOCUS       DAJVRL010000036        32635 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVRL010000036 DAJVRL010000000
VERSION     DAJVRL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.036"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..956)
                     /locus_tag="OQN02_001727"
     CDS             complement(<1..956)
                     /locus_tag="OQN02_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCS1642556.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OQN02_001728"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OQN02_001728"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCS1642557.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OQN02_001729"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OQN02_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OQN02_001730"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OQN02_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1642558.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OQN02_001731"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OQN02_001731"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1642559.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OQN02_001732"
     CDS             complement(5018..6346)
                     /locus_tag="OQN02_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1642560.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="OQN02_001733"
     CDS             complement(6354..8702)
                     /locus_tag="OQN02_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1642561.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="OQN02_001734"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="OQN02_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1642562.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="OQN02_001735"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="OQN02_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1642563.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="OQN02_001736"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="OQN02_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1642564.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="OQN02_001737"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="OQN02_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1642565.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="OQN02_001738"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="OQN02_001738"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1642566.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="OQN02_001739"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="OQN02_001739"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1642567.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="OQN02_001740"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="OQN02_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1642568.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="OQN02_001741"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="OQN02_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1642569.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="OQN02_001742"
     CDS             18273..19172
                     /locus_tag="OQN02_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1642570.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="OQN02_001743"
     CDS             19178..21610
                     /locus_tag="OQN02_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1642571.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="OQN02_001744"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="OQN02_001744"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1642572.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="OQN02_001745"
     CDS             22723..23988
                     /locus_tag="OQN02_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1642573.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="OQN02_001746"
     CDS             complement(24229..25821)
                     /locus_tag="OQN02_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1642574.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="OQN02_001747"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="OQN02_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1642575.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="OQN02_001748"
     CDS             complement(27018..28136)
                     /locus_tag="OQN02_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1642576.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="OQN02_001749"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="OQN02_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1642577.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="OQN02_001750"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="OQN02_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1642578.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /gene="opgE"
                     /locus_tag="OQN02_001751"
     CDS             29186..30769
                     /gene="opgE"
                     /locus_tag="OQN02_001751"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1642579.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="OQN02_001752"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="OQN02_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1642580.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="OQN02_001753"
     CDS             complement(31385..31450)
                     /locus_tag="OQN02_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642581.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..32572
                     /gene="rhtA"
                     /locus_tag="OQN02_001754"
     CDS             31685..32572
                     /gene="rhtA"
                     /locus_tag="OQN02_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1642582.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8167 a         8700 c         7884 g         7884 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggttagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc catggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaagtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    32581 ttctgtatgg ttatgcataa ccatacagaa tttctcgcta cttttcctct acacc
//
LOCUS       DAJVRL010000037        32125 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVRL010000037 DAJVRL010000000
VERSION     DAJVRL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.037"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OQN02_001755"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OQN02_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1642583.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OQN02_001756"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OQN02_001756"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1642584.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OQN02_001757"
     CDS             complement(3736..4092)
                     /locus_tag="OQN02_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1642585.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OQN02_001758"
     CDS             complement(4096..4512)
                     /locus_tag="OQN02_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1642586.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OQN02_001759"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OQN02_001759"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1642587.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OQN02_001760"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OQN02_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OQN02_001761"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OQN02_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1642588.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OQN02_001762"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OQN02_001762"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1642589.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OQN02_001763"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OQN02_001763"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1642590.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OQN02_001764"
     CDS             10521..11504
                     /locus_tag="OQN02_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1642591.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OQN02_001765"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OQN02_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1642592.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OQN02_001766"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OQN02_001766"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1642593.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OQN02_001767"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OQN02_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OQN02_001768"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OQN02_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1642594.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OQN02_001769"
     CDS             complement(15306..15515)
                     /locus_tag="OQN02_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1642595.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OQN02_001770"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OQN02_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1642596.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OQN02_001771"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OQN02_001771"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1642597.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OQN02_001772"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OQN02_001772"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1642598.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OQN02_001773"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OQN02_001773"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1642599.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OQN02_001774"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OQN02_001774"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1642600.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OQN02_001775"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OQN02_001775"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1642601.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OQN02_001776"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OQN02_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OQN02_001777"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OQN02_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS1642602.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OQN02_001778"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OQN02_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS1642603.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OQN02_001779"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OQN02_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS1642604.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OQN02_001780"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OQN02_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS1642605.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OQN02_001781"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OQN02_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS1642606.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OQN02_001782"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OQN02_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS1642607.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8172 a         8246 c         7782 g         7925 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtt actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gcctt
//
LOCUS       DAJVRL010000038        31755 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVRL010000038 DAJVRL010000000
VERSION     DAJVRL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.038"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..1357)
                     /gene="yeaG"
                     /locus_tag="OQN02_001783"
     CDS             complement(<1..1357)
                     /gene="yeaG"
                     /locus_tag="OQN02_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1642608.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1793..2539
                     /gene="mipA"
                     /locus_tag="OQN02_001784"
     CDS             1793..2539
                     /gene="mipA"
                     /locus_tag="OQN02_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1642609.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2629..3390
                     /locus_tag="OQN02_001785"
     CDS             2629..3390
                     /locus_tag="OQN02_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS1642610.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3441..4325)
                     /locus_tag="OQN02_001786"
     CDS             complement(3441..4325)
                     /locus_tag="OQN02_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1642611.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4409..5404)
                     /gene="gapA"
                     /locus_tag="OQN02_001787"
     CDS             complement(4409..5404)
                     /gene="gapA"
                     /locus_tag="OQN02_001787"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1642612.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5746..6159
                     /gene="msrB"
                     /locus_tag="OQN02_001788"
     CDS             5746..6159
                     /gene="msrB"
                     /locus_tag="OQN02_001788"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1642613.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6201..6473
                     /locus_tag="OQN02_001789"
     CDS             6201..6473
                     /locus_tag="OQN02_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1642614.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6843..7919
                     /locus_tag="OQN02_001790"
     CDS             6843..7919
                     /locus_tag="OQN02_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1642615.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7946..9325
                     /locus_tag="OQN02_001791"
     CDS             7946..9325
                     /locus_tag="OQN02_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1642616.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9342..10387
                     /locus_tag="OQN02_001792"
                     /pseudo
     CDS             9342..10387
                     /locus_tag="OQN02_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10408..11244
                     /locus_tag="OQN02_001793"
     CDS             10408..11244
                     /locus_tag="OQN02_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCS1642617.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11249..12196
                     /locus_tag="OQN02_001794"
     CDS             11249..12196
                     /locus_tag="OQN02_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCS1642618.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12206..13186
                     /gene="ydjG"
                     /locus_tag="OQN02_001795"
     CDS             12206..13186
                     /gene="ydjG"
                     /locus_tag="OQN02_001795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCS1642619.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13323..14081
                     /locus_tag="OQN02_001796"
     CDS             13323..14081
                     /locus_tag="OQN02_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1642620.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14199..15557
                     /locus_tag="OQN02_001797"
     CDS             14199..15557
                     /locus_tag="OQN02_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1642621.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15650..16291)
                     /gene="pncA"
                     /locus_tag="OQN02_001798"
     CDS             complement(15650..16291)
                     /gene="pncA"
                     /locus_tag="OQN02_001798"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1642622.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16302..17318)
                     /gene="ansA"
                     /locus_tag="OQN02_001799"
     CDS             complement(16302..17318)
                     /gene="ansA"
                     /locus_tag="OQN02_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1642623.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17485..19341)
                     /gene="sppA"
                     /locus_tag="OQN02_001800"
     CDS             complement(17485..19341)
                     /gene="sppA"
                     /locus_tag="OQN02_001800"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1642624.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19502..20053
                     /locus_tag="OQN02_001801"
     CDS             19502..20053
                     /locus_tag="OQN02_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1642625.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20170..21213
                     /gene="selD"
                     /locus_tag="OQN02_001802"
     CDS             20170..21213
                     /gene="selD"
                     /locus_tag="OQN02_001802"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1642626.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21218..23179
                     /gene="topB"
                     /locus_tag="OQN02_001803"
     CDS             21218..23179
                     /gene="topB"
                     /locus_tag="OQN02_001803"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1642627.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23306..24346
                     /locus_tag="OQN02_001804"
     CDS             23306..24346
                     /locus_tag="OQN02_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1642628.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24463..25806)
                     /gene="gdhA"
                     /locus_tag="OQN02_001805"
     CDS             complement(24463..25806)
                     /gene="gdhA"
                     /locus_tag="OQN02_001805"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS1642629.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26042..26314
                     /locus_tag="OQN02_001806"
     CDS             26042..26314
                     /locus_tag="OQN02_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1642630.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26280..26687)
                     /gene="nudG"
                     /locus_tag="OQN02_001807"
     CDS             complement(26280..26687)
                     /gene="nudG"
                     /locus_tag="OQN02_001807"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1642631.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26774..27394
                     /locus_tag="OQN02_001808"
     CDS             26774..27394
                     /locus_tag="OQN02_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1642632.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27403..28710)
                     /gene="ynjE"
                     /locus_tag="OQN02_001809"
                     /pseudo
     CDS             complement(27403..28710)
                     /gene="ynjE"
                     /locus_tag="OQN02_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28777..29430)
                     /locus_tag="OQN02_001810"
     CDS             complement(28777..29430)
                     /locus_tag="OQN02_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1642633.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29430..30947)
                     /locus_tag="OQN02_001811"
                     /pseudo
     CDS             complement(29430..30947)
                     /locus_tag="OQN02_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30920..31755)
                     /locus_tag="OQN02_001812"
                     /pseudo
     CDS             complement(30920..31755)
                     /locus_tag="OQN02_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7958 a         7601 c         8238 g         7958 t
ORIGIN      
        1 gctgttccag gacgtagaac agatggaccg ggtttgccgc cacttctaca tgatcgaagt
       61 tgaacacgcg ggagaggatc ttaaacgcaa aacgcgtcga cagaccgttc atcccttcat
      121 cgacaccggc gtagtcacga tattcctgat acgacttggc tttcggatcg gtgtctttca
      181 gactttcgcc atcataaacc cgcatctttg aataaatgct ggagttttct ggctctttca
      241 ggcgcgaaag aatggaaaaa cgtgacagtg tttcgagcgt gccaggggcg catggggcgt
      301 gagtcaattc actgtgatta agcaatttct cgtagatttt gatctcttcg gaaatgcgca
      361 agcaatacgg caccttcacg atgtaaacac ggtcgaggaa ggcttcgttg tttttgttat
      421 tacggaaagt gacccattcg gacttgttcg agtgggcgag aataatcccg ttgaacggca
      481 gggcggagat accttccgtc ccgttgtagt taccttcctg agtggcggtt aacaagggat
      541 gcagcacttt tatcggtgct ttaaacatct caacgaattc catgatcccc tgattggcgc
      601 ggcacagcgc accggaatag ccgtaggcgt ccgggtcatt ctgcgcgtag tgttcgagtt
      661 tacgaatatc gactttccca accagcgcgg agatgtcctg gttgttctca tcaccgggtt
      721 ccgttttggc gatagcaatt tgttgcagaa ttgacggcca gaccttcact acccggaact
      781 tagtgatatc gccaccaaat tcatgcaggc gttttgccgc ccatggcgac atgatggtgc
      841 cgagataacg gcgaggaatg ccatactctt tttccagaat ctgagcatct tcctgcggat
      901 tgaaaagaca gaacggatga tcgttgaccg ggctacgctc accgttggcg ctcaatacat
      961 aaatcggtac gagctgcatt aatgatttca gtcgctcagc aagcgatgat ttacccccac
     1021 ccacaggccc cagcagatac aggatttgtt tcttctcttc cagcccctga gccgcgtgtt
     1081 tcagataaga gacaatctgt tcaatcgcgt cttccatgcc gtaaaactct tcaaatgccg
     1141 gataacgtgc aatgacccgg ttagaaaaga gtcgagaaag tctggggtcc tgggctgtat
     1201 cgaccatgac aggctcaccg atagccatca atagccgctc agccgcgttg gcataagcac
     1261 tgcgatcttg ccgacaagtg gtaagaaact cctgcagtgt gaactcttcg tccttggcag
     1321 cttcatatcg ctggcgatag tgatcaaata tattcatggt atgccgtcct ttcgtttttt
     1381 agcacaggtt aagagccgtt catatgaata gtagaggctc ccggaagagg taaactgaac
     1441 gacgtctctt gctaaaacac agcaactctc atgccatttt atcgacgatg agaacaacgt
     1501 gcccggtgat acaccttcaa aattaagcgt agatggcaaa tggaaaactt gcctgcacgg
     1561 ctttcttaat ttcaatggca tatcaacaac tcatccataa attttgcata attaatgtaa
     1621 agaccaggct cgccagtaac gctaaatcca tttggctgta agcgcggtgt catccgcggc
     1681 aggaaaatta aacagttact ttaaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat
     1741 tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa
     1801 actcaaactt ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga
     1861 aggtaaattt tccctgggcg caggcgtagg tgtcgttgag cacccatata aagattacga
     1921 taccgatgtt tacccagtac cggtaatcaa ctatgaaggc gataacttct ggttccgtgg
     1981 cttaggtggt ggttactacc tgtggaatga cgcaacggat aaactttcaa ttaccgctta
     2041 ctggtcgccg ctttacttca aagcgaaaga cagtggcgat caccaaatgc gtcacctgga
     2101 tgaccgtaag agcaccatga tggctggtct gtcttatgct cactttaccc agtacggtta
     2161 cctgcgtacc accctggctg gcgataccct ggataacagc aacggcattg tctgggatat
     2221 ggcctggttg tatcgttaca ccaacggtgg cctgaccgtg actccgggta ttggtgtgca
     2281 gtggaacagc gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag agtccgctcg
     2341 cagcggtctg cgtggctata acccgaatga cagctggagc ccttaccttg agctgagcgc
     2401 cagctacaac ttcctcggcg actggagtgt ttacggtacc gcgcgctaca cccgtctgtc
     2461 tgatgaagtt actgacagcc cgatggtgga taaatcctgg actggcctga tttctaccgg
     2521 gatcacctac aaattctgat aatgcatctt tccagggcat tttgtgcata aaaacagggc
     2581 gtcacgccct gttttgcatt acggtggtgc aatacctggg aggaaacaat gcaacaaaaa
     2641 atgattcaat ttagtggcga tgtctcactg ccagccatag ggcagggaac atggtatatg
     2701 ggcgaagatg ccagtcagcg caaaacagaa gttgctgcac tacgcgcagg cattgaactc
     2761 ggtttaaccc tcattgatac cgccgaaatg tatgccgatg gcggtgccga aaaggtggtt
     2821 ggggaagcat taaccggtct gcgagagaag gtctttctcg tctctaaagt ctatccgtgg
     2881 aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg tctcaatact
     2941 gactatctcg atctctactt attacactgg tctggcagtt tcgcttttga agagactgtt
     3001 gccgcgatgg aaaaattgat cgcccaggga aaaatccgtc gctggggcgt ttctaacctt
     3061 ggttatgccg atatgcagga actctggcag ctgccggggg gaaatcagtg tgccactaat
     3121 caggtgcttt accatctcgg ttcacgaggg attgagtacg atctactccc ctggtgccag
     3181 caacagcaga tgccggtgat ggcttacagt ccattagccc aggccgggcg gttgcgcaat
     3241 ggactgttaa aaaacgcggt agtcaacgaa attgcacatg tccaacaaca tgcagctgcg
     3301 cttgaggtcg aactttcgtc agcggaatta gctatgctgg ataaggcata tccggcacca
     3361 aaaggaaaaa ctgcgctgga tatggtgtga tgttgatacg tgccggatgc atcgcacccg
     3421 gcacacaaca ttaacgcaaa ttaacgtttg gcaacgcgaa tggattgcgc cagatgtgca
     3481 ggtttctctt tggtgacttt ttgcgtttct gaagcgtaag ccgtttctac acaaacaaag
     3541 gtcttgtagc catcgtccgg catgtcgccc atgctaactg aaagcgccgg tcccgggttc
     3601 cagccgacaa cgttcagatg atgctggtgg cctacggcga taatacgatt cagcgcttca
     3661 tcattaatca cgctgcaatc ttgcggattc agatacacgc gatcggtacg gtcagggaag
     3721 gtctgaatac cgtcggtcag tacatcttct ttcgcgtcat tcactttatc aatgaagcga
     3781 tcgcccagcc cactgacgct taccttagcg atatcaccca cgttaaagta ggtatgcagg
     3841 gcagaggtgg tttcaaattc gccatgtgat tcaagatcga tttcacaggt tttacccacg
     3901 cggaaatgcg ctaacagcgt aaagtcgtgc ggccagaatt ttttcgtctc ttcgctttgt
     3961 gtcaattcaa aagtcagcgc tacgccatca gcatcttcat gatgtgattt cagcgtccac
     4021 ggcaggttgc gggcaaaacc gtgcgcaggc agaccttgtt gtgccgccgg accaaaccac
     4081 ggccagcaaa ccggtacgcc accgcgaata gcgacgccat ttttgaacgg tgtgttgttg
     4141 ctcaaccaca gaacttcttc ttcacccgca ggtttccacg agagaaggtg tgcgccctgt
     4201 aatgcaaaag aggcttttac ctggggatga tcgaccacaa tgaggtccag ttcatccagt
     4261 ttacgacggg agaggacagg ggagatttgt tcgatgaccg gaagggcaaa aattttctta
     4321 atcatgacgc agtcctttaa cttcatttta tcaggtaaaa aaaagagcga ccgaagtcgc
     4381 tctttttaga tcacagtgtc atctcaactt atttggagat gtgagcgatc aggtccagaa
     4441 ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg aagttgtcgt
     4501 tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg ttgaaatcgg
     4561 tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct tcagcagcag
     4621 ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga acggtcaggt
     4681 caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt ttgccattca
     4741 gttctggcag tactttacct acagctttag cagcaccggt agaggacggg atgatgttct
     4801 gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg gttttctgag
     4861 tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag ttatcgttga
     4921 taactttagc cagcggagct aggcagttgg tggtgcagga agcgttggaa acgatgtcct
     4981 ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg tctttagacg
     5041 gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca gtttcgtcag
     5101 tcaggaacag accagttgct tcagcgacaa cgtcaacacc cacttcgtcc catttcaggt
     5161 tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc agatgaccgt
     5221 ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc agcatgtatg
     5281 ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca gaacgtttct
     5341 gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct actttgatag
     5401 tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa ttacaaaaac
     5461 cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc tgtgcctaag
     5521 cattacgcga ctgactcgcc tcacttttcc tttgggcttg agaccacatg gggtcggcgc
     5581 cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt taggtgcagc
     5641 catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat gttttgttag
     5701 aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agtaaatggc gaataaacct
     5761 tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac gcagaatcat
     5821 gggacagaac cgccatttac gggtcgttta ctgcataaca agcgtgacgg cgtatatcac
     5881 tgtttgatct gcgatgcccc gctgtttcat tccgaaacca agtatgattc cggctgtggc
     5941 tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa agacttgtca
     6001 catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct ggggcatgtc
     6061 tttcccgacg ggccgcaacc aacaggcgaa cgttattgtg ttaactctgc ttctttacgc
     6121 ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc agcaacttat
     6181 tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg acgcctgagg
     6241 tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcctgatggc gttgcgttga
     6301 ccgaggaaca aaaagagaat tgcctgcaac tggtgatgct gtggcaagcc cgccataata
     6361 ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag agcaaacagc
     6421 agttaaaaga agattttggt atctcagcaa agcctattgc catgtttaag taagtggttc
     6481 atcccctttt tgccgcggct atgaagggga tagccaacaa tgaccccacg actcgtctca
     6541 attatctcga cccgacctgg ctaagtccca gtccgatctc ttgcagtcgc gttcacgcct
     6601 gaatgacttc tcctgaactt aagtctcaga cctatttggc cggtaatccc tctcgaattt
     6661 gctctttttg cgtcccgcct tccaccaacc tgtaacagac ccagccattt tctgcgtaat
     6721 tgtgatctga gttgtaaaat gcaggtcatt atctgtgttg tgcagattta atattgacct
     6781 caaaaatcat gtgggtaagt atgatggcca tctaagcaaa acaaaacaca gaggatgacg
     6841 caatgaaagc actggctcgg tttggcaagg cctttggcgg ctacaagatg attcatgtcc
     6901 cacaacccat ttgtggcccg gaagacgtag tgattgaaat taaagccgcg gcaatctgcg
     6961 gcgcagacat gaagcactac aatgtcgata gcggttctga tgagtttaac tctatccgcg
     7021 gccatgagtt cgcaggttgt attgcgcagg ttggtgaaaa agtcaaagac tggaaagtgg
     7081 ggcaacgcgt cgtgtcggat aacagcggtc acgtttgcgg cgtttgtccg gcctgtgaac
     7141 agggtgattt tctgtgttgt acagaaaaag taaaccttgg tctggataac aatacctggg
     7201 gcggtggttt ttccaaatat tgtctggttc ctggtgaaat tctcaaaatt catcgtcatg
     7261 cgttgtggga aatccctgat ggtgttgatt atgaggacgc agccgtactt gaccctatct
     7321 gtaatgccta taaatccatc gcgcagcaat cgaaattcct tcctggtcag gatgttgtcg
     7381 tcattggcac tggcccactc gggctgtttt ccgtacaaat ggcgcggatt atgggggcgg
     7441 taaatatcgt cgtcgttggt ctgcaagaag atgtggcggt ccgcttcccg gttgcaaaag
     7501 aactgggggc gacggcagta gtaaatggtt ctaccgaaga tatggtggcg cgctgccagc
     7561 aaatttgtgg caaagataat ctgggactgg tgattgaatg ctccggtgcc aatatcgcac
     7621 tgaaacaagc catcgaaatg cttcgcccga atggggaagt ggtacgcgtt ggaatgggct
     7681 tcaaacctct tgatttctcg attaatgaca ttaccgcctg gaataaaagc atcattgggc
     7741 atatggccta tgactccacc tcatggcgta acgctatccg gctattagcc agcggcgcta
     7801 tcaaagtcaa accgatgatc acgcatcgta tcggcctgtc gcaatggcgc gaagggtttg
     7861 atgcgatggt cgataaaacc gcaatcaaag tgatcatgac ttacgacttt gatgaataac
     7921 tactctcctg aaggaacgtt atcgcatgga acagataaca aaaccgcatt gtggtgcacg
     7981 gctggatcgc ttaccggatt gccgctggca ttcgtcaatg tttgccatcg tcgcgtttgg
     8041 cttgcttgtc tgctggagta atgccgttgg tggcttgatc ctcgcgcagc tgaaagcgtt
     8101 gggctggaca gataattcca ctacagccac attctcagca atcacgaccg ccggaatgtt
     8161 tctcggtgcg ctggtgggcg gcatcattgg cgacaaaacc ggtcgcagaa atgcgttcat
     8221 cctctatgag gccattcata ttgcctcgat ggttgtcggt gctttctcac cgaatatgga
     8281 ttttcttatt gcctgccgtt ttgtgatgga cgttgggctg ggagctttac tggttacact
     8341 gtttgctggt ttcaccgaat atatgcccgg tagaaatcgc ggaacgtggt ccagtcgggt
     8401 ttcctttatt ggcaactggt catatccgct ctgctcactg attgcgatgg gactcacgcc
     8461 gctgattagt gcagagtgga actggcgggt acaactgctt attcctgcaa tattgtcgct
     8521 tatcgctacg gcgctggcct ggcgctactt tcctgaatcc ccgcgctggc tggaatcgcg
     8581 cggacggtat caggaagccg agaaagtcat gcggagtata gaagaaggcg tcatacgcca
     8641 gacgggtaaa cctttgccgc ctgtggttat tgctgatgac ggtaaagcgc cacaagcggt
     8701 gccgtattca gccttactga caggagtatt actgaaacgc gtgatattag gctcttgtgt
     8761 gctgattacc atgaacgttg tgcagtacac actaattaac tggttgccaa caatattcat
     8821 gacccagggg attaatttaa aagactcgat tgttttaaat accatgagta tgtttggcgc
     8881 gccatttggt atttttattg ccatgctggt gatggataaa attccgcgta aaacaatggg
     8941 tgtggggcta ttaatcctga ttgcggtgct cggatatatc tattcactgc aaaccagtat
     9001 gttgctcata acgctgattg gtttcttcct gattactttc gtctatatgt acgtttgcta
     9061 tgcctcggca gtgtatgtcc ctgaaatctg gccgacagag gccaaactcc gtggctccgg
     9121 tctggcgaat gcggtagggc gaatcagtgg tattgccgca ccttatgccg ttgcagtgct
     9181 gctcagtagt tatggtgtaa cgggagtctt tattcttctg ggggctgttt caaatattgt
     9241 cgcaattgct atcgccacca ttggaattga aaccaaaggt gtctccgttg aaagtttaag
     9301 tattgatgca gtagccaata aataatacgg gtggattcaa aatgaaaaat tcaaaagcaa
     9361 tattgcaggt gccgggcaca atgaaaatta tttcagcaga aataccagtg cctaaagaag
     9421 atgaagtttt gattaaagta gaatatgtcg gtatttgtgg ttcagatgta catggttttg
     9481 aatcaggccc gtttattccg cctaaagacc caaatcaaga aattggcctg ggtcatgaat
     9541 gcgccgggac ggttgtggct gtgggaagcc gcgtgcgcaa atttaaaccg ggggatcggg
     9601 taaatatcga acttggcgtt ccttgcggtc actgtcgtta ctgtctggaa ggcaaatata
     9661 acatctgccc ggacgttgat tttatggcga cacaacccaa ctaccgcggc gcattaacgc
     9721 actatctgtg tcatccggag agctttactt acaaactgcc cgacaatatg gacacgatgg
     9781 aaggggcgct ggtggagcct gccgcagtcg ggatgcatgc cgcgatgctg gcagatgtta
     9841 aaccgggtaa gaagataatt attctgggag caggttgtat tggtttgatg acgttgcaag
     9901 cgtgcaaatg cctgggcgca acggaaattg ccgtcgttga tgtactggaa aaacgtctgg
     9961 caatggggga acagcctggt gcgacagtgg ttattaatgg cgcaaaagag gacactattg
    10021 cacgctgtca gcaatttacc gaagacatgg gcgcagatat tgttttcgaa acagcgggtt
    10081 ctgcggtcac cgttaaacag gcacctttaa tctggtaatg cgcggcggta aaattatgat
    10141 tgttggtact gtacccggcg attcggcaat caatttcctc aaaatcaatc gcgaagtcac
    10201 tatccagacg gtattccgct atgccaatcg ttatccggtc acgattgaag ctatttcttc
    10261 agggcgattc gatgtgaaat cgatggtgac gcatatttac gattatcggg atgtacaaca
    10321 ggcatttgaa gagtcagtta acaacaaacg cgacattatt aaaggcgtta ttaaaattag
    10381 cgattaatta atacgggaga acagaacatg ctcgcagata tcagatattg ggaaaacgat
    10441 gccacaaata aatattatgc aattgcccat tttaacgtat ggaatgcaga aatgttgatg
    10501 ggcgttaaag acgcagccga agaagcgaaa tccccggtta ttatttcttt tagtacaggt
    10561 tttgtcggta acacctcatt tgaagatttc tctcacatga tggtatcaat ggcacaaaaa
    10621 gcaacggtgc cggtaataac tcattgggat catggtcgga gtattgagat tattcataac
    10681 gcctggactc atggtatgaa ttcattaatg cgtgatgctt ctgcatttga tttcgaagaa
    10741 aatattcgtt taaccaaaga ggctgttgat ttctttcatc cgctgggtat tccggtagag
    10801 gcggaattag ggcatgtcgg taatgaaacc gtttatgagg aagcgctggc gggctatcac
    10861 tataccgatc ctgaccaggc ggctgaattt gttgaacgta cgggctgtga ctcattggct
    10921 gtcgccatcg gcaaccagca tggggtttat acgtcagagc cgcaattgaa ctttgaggtc
    10981 gtcaaacgcg tacgcgatgc tgtttctgtt ccgctggttt tgcatggcgc atcggggatc
    11041 agtgatgccg acattaaaac tgcaatttcg ttgggtattg cgaaaatcaa cattcatacg
    11101 gagctctgtc aggccgcaat ggtagcagtg aaagaaaacc aggatcagcc tttcctgcat
    11161 ttagaacgtg aagtacgtaa agctgtaaaa gaacgggcat tggaaaaaat tgaactgttc
    11221 ggttctgatg gcaaagcgga ataatgatat ggataatctc gacgttattt gtataggtgc
    11281 cgctattgtt gatattccat tacaaccggt cagtaaaaat atctttgatg tagattctta
    11341 tcctcttgaa agaatcgcaa tgagcaccgg gggcgatgca ataaatgaag caacaattat
    11401 ttctcgtctg gggcatcgta ccgcattaat gagccgcatt ggtaaagatg cctcagggca
    11461 atttattctc gaccattgtc gcaaagagaa tattgatatt caaagcctga aacaggacgt
    11521 cagcatagat acatctatta acgttggact ggtgacggag gatggcgagc ggacgtttgt
    11581 cacgaaccgt aacggcagtt tgtggaaatt aaatattgac gatgttgatt ttgcgcggtt
    11641 tcctcaggcg aaattattat cactggccag tattttcaac agtcctctat tggatggtaa
    11701 agcgctaaca gaaattttta cgcaagcgaa agcccggcag atgatcattt gtgccgatat
    11761 gatcaaaccg cggttgaatg aaacgctgga tgatatttgc gaagcattga gttatgtcga
    11821 ttatctgttt ccaaattttg ccgaggaaaa attactcaca gggaaagaga cactggatga
    11881 aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg gtgattaaaa cgggtaaaga
    11941 cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg ccggcggtcg caggaataac
    12001 cgccatcgac accattggcg cgggcgataa ctttgcttca ggttttattg cggcactgtt
    12061 agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat gcaacggcgg ctatctcggt
    12121 tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag ctggtcgaac aattgctgga
    12181 agaatacgaa ggataatgaa ggcaaatgaa aaagatacct ttaggcacga cggatataac
    12241 gctttcgcga atggggttgg ggacatgggc cattggcggc ggtcctgcat ggaatggcga
    12301 tctcgatcgg caaatatgta ttgatacgat tcttgaagcc catcgttgtg gcattaatct
    12361 gattgatact gcgctaggat ataactttgg caatagtgaa gttatcgtcg gtcaggcgtt
    12421 aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa tgcggcattg tctgggaacg
    12481 aaaaggaagt ttattcaaca aagttggcga tcggcagttg tataaaaacc tttccccgga
    12541 atctatccgc gaagaggtag aagccagctt gcaacgtctg ggtattgatt acatcgatat
    12601 ctatatgacg cactggcagt cggtgccgcc attttttacg ccgatagctg aaactgtcgc
    12661 agtgcttaat gagttaaagg ctgaagggaa aattcgcgct ataggcgctg ctaacgtcga
    12721 tgctgaccat atccgcgagt atctgcaata tggtgaactg gatattattc aggcgaaata
    12781 cagtattctc gaccgggcaa tggaaaacga actgctgccg ctatgtcgtg ataatggcat
    12841 tgtggttcag gtttattccc cgctagagca gggattgttg accggcacca tcactcgtga
    12901 ttacgttccg ggcggcgctc gggcaaataa agtctggttc cagcgtgaaa acatgctgaa
    12961 agtgattgat atgcttgaac agtggcagcc actttgtgct cgttatcagt gcacaattcc
    13021 cactctggca ctggcgtgga tattaaaaca gagtgattta atctccattc ttagtggggc
    13081 tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg aatatcaact tatcggatgc
    13141 agacgcaaca ttgatgaggg aaatggcaga ggccctggag cgttaaatac tcaacacgat
    13201 tcacctctgt ttctgtgaaa actgtgatga aaatctgtga ttcaaatagg ttattttgaa
    13261 agtaaacatc ggttatgcga taatcgcgct aatgtgatgt aaaaagatac aggggtgtaa
    13321 tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat ggttgccaac gataaaaagg
    13381 tgacagtctc aaatttgagt gggatttttc aggtaaccga agaaaccatt cgccgcgatc
    13441 ttgagaagct ggaagatgaa ggctttttga ccagaaccta tggtggtgct gttttaaata
    13501 cagcggtgtt gacggagaat atccattttt ataagcgcgc ttcatcgttt tatgaagaga
    13561 agctgcttat tgcacgcaag gcactaccct ttatcgacaa taaaaccacc atggcagccg
    13621 attccagtag taccgtaatg gaattgctca aattgttaca ggaccgtagt gacctgacgt
    13681 tgctaaccaa ctcggcggaa gcgattcatg ttctggctca gtcagaaatt aaagtcgttt
    13741 caacgggtgg ggaactaaac aaaaatacac tttccctgca aggaagaatt actaaagaga
    13801 tcatcagccg ctaccatgtc gatatcatgg taatgagttg taaaggtctt gatattaaca
    13861 gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa aaagacaatg atccgtcagg
    13921 cgacagaagt tgcgttattg gttgatcact ctaagtttga tcgtaaagct tttgtccagt
    13981 tagctgactt tagtcatatt aattatataa taactgataa atcaccgggt gcagagtgga
    14041 tagcattttg caaagacaat aatatccagc tggtgtggta atttaagcac acgttgtcaa
    14101 tattatttgt tcctgcgact ggaaataaaa tgatgataaa gatgcggcaa gtgaatttgc
    14161 cgcaattgct accttttata gcaaatccga ggcacccaat ggaacaatat gatcaaattg
    14221 gcgcaagact ggaccgcttg cctttggccc ggtttcatta tcgtatattt ggtataataa
    14281 gctttagtct gttattaacg gggtttttga gttactcagg taatgtcgtc ttagcaaagc
    14341 tggtaagcaa tggatggtca aataatttcc tctatgccgc ctttacctcg gcattaatgt
    14401 ttggttattt catcggctca cttactggtg ggtttattgg tgactacttt gggcggcgca
    14461 gggcgtttcg cataaatctt ctcatcgtcg gtattgctgc aacaggggcc gcttttgtcc
    14521 ctgatatgta ctggctcatt ttctttcgct tcctgatggg aacaggaatg ggggcgctga
    14581 ttatggttgg ctatgcctca tttacggagt ttatccccgc gacggtgcgt ggaaaatggt
    14641 ccgcgcggct ctcatttgtt ggtaactggt cgcccatgct gtctgcggcg ataggcgtgg
    14701 tggttatcgc tttttttagt tggcgaataa tgtttcttct gggtggtatt ggcatactgt
    14761 tagcctggtt tctctcaggt aaatacttta tcgagtcgcc acgatggctg gaagggaaag
    14821 ggcaaatcgc cggtgcagaa agccaacttc gtgaagtaga gcagcaaatt gaaagggaga
    14881 agagtattcg tttaccccag cttactttga accagagcaa cagcaaggtt aaggtaatca
    14941 agggtacctt caggctcctg tttaaagggg aaatgttacg acgtacatta gttgcgataa
    15001 ctgttttaat tgcaatgaac atttcgcttt ataccattac cgtatggata ccgaccatat
    15061 ttgttaactc cggcattgat gtcgataaat caatattaat gaccgctgtt attatgattg
    15121 gtgctccggt aggaatattt attgcagcat taattattga tcattttcct cgtcgattat
    15181 ttggctccgc cttacttatt attattgccg tgttaggcta tatctattca agtcagacta
    15241 cagagtgggc gattttaatc tatggtctgg tgatgatctt ctttttatac atgtatgttt
    15301 gcttcgcgtc ggcggtttat atcccggagc tttggccaac gcatttacgc ctgcgcggtt
    15361 cgggtttcgt taatgccgtc ggacggatcg tcgcagtttt cacgccctat ggcgttgcgg
    15421 cattattaac acattatggg tcgatcacgg tgtttatggt gcttggtgtc atgttagtgc
    15481 tctgtgcgct ggttctctcc atttttggca tcgaaacgcg gaaggtgtcg ctggaagaga
    15541 tttctgaggt gaattaaatc tttcgttgat ggcatcaatt gccagatgta ccactgacgt
    15601 gttttatttg gcctacaatg ccagtacaga attgtaggcc agcgtaaaat tacccctgcg
    15661 tctcttccca gtctgccagc gtatatagcg ttgccccagc tgctgacatc tccataaacg
    15721 cgtgtgcact gtcctggggc tggatattca cgccacgaca gccatcggta atcacgttta
    15781 ccttataacc taactgtaac gcgtccagca cggtaaactt cacgcaatag tcagtagcca
    15841 ggcccataac gatcaattca tcgatttcat gatcgcgcaa ccagtcatca agcgcggttt
    15901 tctgccgacg tccgttatca aaaaaggcac tgtaactgtc aactaaagga ttttcgcctt
    15961 tatggaacac cgctgcgatc tctttttgat tcagtaacgg atgtaattgt gcgccttcac
    16021 tgttctgcac acagtgttca ggccagaaag tttgccgcag gccgccgagt tggcctggcg
    16081 tataaggctc tacgccgtgc tggctggcaa agctgccgtg attcgccgga tgccagtcct
    16141 ggctggcgat aaccgcttct ccgcgcgact ggcaccagtc aataaggcgg ttagcaacct
    16201 ccaccgtact gtcaccttcc ggtacggcga gcgcgccacc agcacagaaa tcattttgta
    16261 aatcgaccag taacagggcg cgatggggca ttgcagtctc cttaatcatc cggcgtcagt
    16321 tcgccgcgca ggttttggct catggccttg cgaatggttt cagtatccag ttcctggctc
    16381 agcaggtaat gcagtttggt tagcgtggct tctacagtca tatctgcacc gtcaataacg
    16441 ccggcatggg cgagggcgtt accggtggca taaccactca tattcacttt accggacata
    16501 cattgtgtca ggttgaccac cacaataccg cgatcgctgg cttcttgtaa ttcctgcagg
    16561 aaggctttgt tttgtggcgc attacccacg ccataggagc gcagaatcaa tgctttcacc
    16621 ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc ctggataaat cgtcaccacg
    16681 ccaattggtt gtggcgtgat tggatgaacg attaattccc cttcaccgtg cggggcgggt
    16741 ggcgtattca aacgacgaat atggatacca gcttccagta acggaggaag gtttggagag
    16801 gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc ggttgccgcg atacaatcgg
    16861 ttattgaaaa agagcgttac ttcgttgatc ggataattcg ccgcaacgta caacgcattc
    16921 agcagattaa tttgtccgtc agagcgtaac tcagccagcg ggatttgtga ccctgtcaca
    16981 atgaccggtt taccgagatt ctcgagcatg aacgacagcg cagaggcggt atacgccatc
    17041 gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt catagtgcgc tttaatatct
    17101 tcagcaatat gctgccagtc ttccggcgtc atatcagaag aatccatcaa cggcgtatat
    17161 tcatgaatgg tgaaatctgg catctccggg cgatggaatt ccggcatcag cgccagttgg
    17221 cgttgtagat gacctgacac cggaatataa ccctgctcgg aacgctgcat cccgatggtc
    17281 ccgcccgtgt aggcgacgta aattgatttc ttttgcatga tattgatatg aatgatatcg
    17341 aaagagcaaa agtatatacg tgaggcagga ggagcgcacc tgatactatg cggataagga
    17401 aatatggggc gagacagcgg gaagaggatt cgtcgaggta aaaaataatg tggccgacga
    17461 tctgtcggcc actcagtaca agacttaacg cacgttggcg caggtcaggc aaaacgcata
    17521 acggttttgt gggtcgttaa acgcggccag cttgtcgctt tcacttttaa cggtagaggc
    17581 taccgaggca agcggtgcag gtaacatggc ctggaacgca tctggcaaca ttgcccggac
    17641 agaaccagac atgttgtcca tcactttgtc gaagaaggtc ggttcatcaa cgtagtattc
    17701 cagatgccac tgtttcactt ttgcgagctc tgctgctttg gcaaccgcat catcgaaatc
    17761 cccgagacta tcgaccagcc cgttagcttt tgcatcctga ccggtccaga cgtggccctg
    17821 ggcgatttta tcaatttgct ccggcgtcga atgacgcgca tcagcaacca gcgtgataaa
    17881 gcgtttatag ccattctcaa tgcttaattg catcatctgc tgcgcttccg gcggcagtgc
    17941 cctggtgata gaaacatccg ccagcggtga agttgagaca ccatcagtat gaacaccaat
    18001 cgaatccaga ctattttcta cggtggtgat cacgccgaag ataccgatag aaccggtcag
    18061 ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa atccagtaac caccagatgc
    18121 cgccatgccg cccatcgata caaccacaga cttacccgct gcccgggctg ctgccagttc
    18181 agcgcgaatc acttcagacg cggtaacgct gccgcctggg ctattaacac gcaggacaat
    18241 cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt gccgcagtgg tatcaccgcc
    18301 aacattcccc tgagtttcct cgccatccat aattgcgcca ttagcgaaga cgacaccgat
    18361 gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg taataactga tggcgcgata
    18421 atttttatca gtcttactcc agccgaactc tttggtcaga gctttttcga tttcggcact
    18481 cgatgccagt gcatcgacca gcttgttttc cagtgcatat ttcgcggtat cgccaccggt
    18541 tttggttaaa ccctcaagca acccttgcgc gccagggaat acctgctgag cagggatctg
    18601 ccggttagcg gcaacagtat tcagatagtt ttgccacagc tcaccaatcc agcggctgtc
    18661 agcttcgcgg gctgccggtg acatatcatc acgaataaac ggttcaacgg cagatttata
    18721 cgtacccacg cggaacacat gggtggaaac tttcagctta tccagcaacg atttgtagta
    18781 cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct tgcggtgaca gccaaatttt
    18841 attggcgaaa ctggcgagat aatattgccc ctggctgtag ttctcgccaa cggcataaac
    18901 cggtttcccg ctgtcacgaa actctttcag ggctttgccg atgtactgca tagacggctg
    18961 gtcgccgcct gcgaagtttt tcagatccat cacgataccg gtgatattgc ggtcgtcctt
    19021 cgcctggcga atggtgttga cgatatcaaa cagtgagttt tcctgcagac gatcggaact
    19081 ggcaccaagc agctggcggc ttaatttact aaaccgctga gaactgtctg gtttatcgac
    19141 gatcacacca gaaatgtcca gcagcagtgc gccacgactg gccgtttctt tcgaatcacc
    19201 accactgacc tgcatccaga tccccacacc aaccagtacg aggaaaataa agaacaggtt
    19261 aagtaccatt tcacggacga aattcagcag acgccacgtc catttaaaaa atccggcaat
    19321 aaatcgccaa agggttcgca tgtattctcc caacttaagg tcacacctgt cacaggcgca
    19381 atagatgtgg ttatcgtaat gacccaaccg gcacttgtca gcaggaattg cggactgtgc
    19441 tgtaacaaaa tccactctcg tgttaatttt gtgaataaat atcacgacag gagttaatca
    19501 aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc tcccgcctgg ctgaacccgc
    19561 gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt atgcgtgcgc cggaccataa
    19621 gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa gggtgcgagc gtttcagcgc
    19681 cgtactggaa cagggggcga ttgctgccgg tagtgatgat aaagctatcg acaaagcccg
    19741 taatgcgccg ttccgcgcac cgctcatcat cacggtggtg gcgaaatgca aagaaaatca
    19801 taaagtcccg cgatgggaac aggaaatgtc tgccggatgc gcggtcatgg cgatgcaaat
    19861 ggcagccgtt gcccagggat ttggcggcat ctggcgcagt ggcgcattaa ctgaaagtcc
    19921 ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa attgtcggtt ttctctacct
    19981 cggcacgccg cagttgaaag catctacgtc gattaacgtc ccggacccga cgccgtttgt
    20041 aacttatttc tgataattgt cgcgaaactg tctggattgt gagcaaaggc gcaggaattc
    20101 agacactctc acttatcact tcacggaatg agggttacca tacccggatt gcattgacag
    20161 gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat acagccacgg agctggttgc
    20221 ggctgtaaaa tttccccaaa agtgttggaa accatcctgc acagtgagca ggcgaagttt
    20281 gttgatctga atttgcttgt gggtaatgaa acccgcgacg atgcggcggt gtacgatctg
    20341 ggcaatggca ccagcgttat cagtaccacc gacttcttta tgccgatcgt tgataatcct
    20401 ttcgattttg gccgcattgc ggcgactaac gccatcagcg atatcttcgc gatggggggc
    20461 aaaccgatta tggcgattgc gatcctcggc tggccgatta acaaactttc cccggaaatt
    20521 gcccgcgaag taaccgaagg tggacgctat gcatgccgtc aggcgggtat tgcgctggct
    20581 ggcggtcact ccatcgatgc gccggagccg atttttggtc tggcggtaac ggggatcgta
    20641 ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg gatgcaaact gttcctgacg
    20701 aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa aatcactgtt gaaaccagaa
    20761 catcagggac tggcgacgga agtgatgtgc cggatgaaca tcgcaggcgc gtcctttgcc
    20821 aacatcgaag gcgtaaaagc gatgaccgac gttacgggct ttggtctgct gggccacttg
    20881 agcgaaatgt gtcagggggc tggtgtgcag gcacgcgtcg actatgaagc gatcccgaaa
    20941 ctccccggtg ttgaagagta cattaagttg ggcgcagtac ctggcggcac tgaacgtaac
    21001 tttgccagct acggtcatct gatgggtgaa atgccgcgtg aagtgcgcga tctgctgtgc
    21061 gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc cggaagcaga aaatgaggtt
    21121 aaaactacag ccgccgagtt tggcattgaa ctgacggcga ttggcgaact ggtgccagcg
    21181 cgcggcggtc gtgccatggt tgagatccgt taatttaatg cggttgttta ttgccgaaaa
    21241 accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa ccgcaccgga aaggcgatgg
    21301 ctttatcgag tgcggtaatg gtcaggtggt gacttggtgt atcggtcacc tgcttgagca
    21361 ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg aatcttgcgg atttgccgat
    21421 tgtcccggaa aagtggcaat tacagccacg accctccgtg accaaacaac ttaacgtcat
    21481 caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc ggggacccgg atcgtgaagg
    21541 gcaattgctg gtggatgaag tgctggacta tctgcaactg gcaccggaaa agcgccagca
    21601 ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg gttgagcggg cgatcgaccg
    21661 tcttcgttcc aacagtgagt ttgtgccatt gtgcgtttct gcgctggcgc gagcgcgtgc
    21721 cgactggctg tacggcatca atatgacccg tgcgtatacc attctcggtc gcaatgccgg
    21781 ttatcagggc gtactttccg tgggacgcgt gcagacaccc gtgctcgggc tggtggtgcg
    21841 ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc tttgaagtca aagcacatat
    21901 cgtgacacct gccgatgagc ggtttaccgc tatctggcaa ccgagcgaag cgtgtgaacc
    21961 gtaccaggat gaagaagggc gcttgttaca tcgtccactg gcggagcatg tggttaaccg
    22021 cattagtggt caaccggcta ttgtcaccag ctataacgat aaacgggaat cagaatccgc
    22081 gccgctgccg ttttcgcttt cggcgttgca gattgaagcg gcaaaacgct tcggtctgag
    22141 cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa acgcacaagc taatcactta
    22201 tccgcgttcc gattgtcgct atttgccaga agaacatttt gccggacgcc acgcggtgat
    22261 gaatgccatc agcgttcatg caccggatct gttgccgcag ccagtggtag atccagatat
    22321 acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac cacgccatca tcccgaccgc
    22381 acggagttct gcgatcaacc tgacggagaa cgaagcgaag gtctataacc tgattgcccg
    22441 tcaatacctg atgcagttct gcccggatgc ggtgttccgc aagtgtgtta tcgaactgga
    22501 cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt gctgaagcag gctggcgcac
    22561 gctgttaggc agcaaagagc gcgatgaaga aaacgacggc acgccattgc ctgtggtggc
    22621 gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta gagcggcaaa cccagccgcc
    22681 gcgccatttt accgatgcaa cactgctttc ggcgatgacc gggatcgcgc gctttgtgca
    22741 ggacaaagat ctgaaaaagg tccttcgtgc gaccgatggt ctggggacag aagcaacccg
    22801 tgccgggatt attgaactgc tgttcaagcg tggttttctg accaaaaaag ggcgctatat
    22861 ccactccacc gacgccggaa aagcgttatt ccattcgctg ccagaaatgg cgacgcgacc
    22921 ggacatgacc gcgcactggg aatcggtgct gacgcaaatc agcgaaaagc agtgtcgcta
    22981 tcaggacttt atgcagccgc tggtggggac gctatatcag cttattgatc aagccaaacg
    23041 tacgccggtg cggcagtttc gcggcattgt ggctccgggc agtggtggca gtgctgataa
    23101 gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt ccgccagcag atgaagccgg
    23161 aagcggggcg atagcgtaag cgagcgaatc tttcgtgcta ttcgagtcat attctgaaat
    23221 atccagcgga tcaagaaaat tcgttggata tttttttgca tggataaaat tatcgcctct
    23281 aaagtatgta ataacaggga atgtgatgaa aaaagtttta ttgcaaaacc atcctgggag
    23341 cgagaagtat tcttttaatg gctggggaat atttaatagt aattttgaac gggtgattaa
    23401 agaaaataag gccatgctgc tttgtaagtg ggggttttat ttaacatgtg ttgtcgctgt
    23461 aatgtttgta ttcgcagcga taacatccaa cggtttgaat gaaagaggcc tgattaccgc
    23521 gggatgctct tttctttatc tattaattat gatggggctt attgttcggg ccggttttaa
    23581 agcaaaaaaa gaacaactgc attattatca ggctaaaggt attgagccgc tcagtatcga
    23641 gaagttacag gcgctacaat tgatcgcacc ttatcgattc tatcataagc aatggtctga
    23701 aacgctagag ttctggccgc gaaagcctga acctggcaaa gataccttcc aatatcatgt
    23761 gcttcctttt gactcgatcg atatcatcag taaaagacgc gagtctttag agaatcaatg
    23821 gggtatcgaa gatagcgaaa gttattgtgc cttaatggag cattttcttt ctggcgacca
    23881 tggagccaat acctttaaag caaacatgga ggaagcccca gagcaggtta tcgccttgtt
    23941 gaataaattt gctgtttttc cctcagacta tatctctgat tgcgctaata atcgctccgg
    24001 taaatcctcg gcgaagctaa tatgggcggc ggaattatca tggatgatct cgatatcaag
    24061 cacagctttt caaaacggga caattgaaga agaactggcc tggcattata taatgcttgc
    24121 ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat tatcaaaaaa atagtctaat
    24181 gggatttctt tactggcata tctgctgcta tcgcagaaag ttaacggatg ctgaacttga
    24241 agcgtgttat cgttacgaca agcagttttg ggagcactac agtaagaaat gccgttggcc
    24301 cataagaaat gttccctggg gagcatcatc cgttaaatac tcataaacgc ctgaaatttt
    24361 gccgggggcg ctgcgcttga ccggcctaca ttagagcgta actgcaatga attgtgccca
    24421 tctgtaggcc tgataagcgt agcgccatca ggcatttaca acttaaatca caccctgcgc
    24481 cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt
    24541 ttgctcacct tcaccaccat gctcaacaca ggcatggtgg atatccagca tgatgtgatg
    24601 caaacgtgcg tcaactttct cggctttcca gcccaggcgc gcagcgtttt gtgccatttc
    24661 caggcccgac gttgctacac cgccagcatt agccgcttta cccggtgcaa acagtacgcc
    24721 tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc atatttgccc cttcagcgac
    24781 ggctttaacg ccattggcga taagctgatg cgcggcgtca acatccagtt cgttctgggt
    24841 ggcgcaaggc agggcgatat caaccggtac agaccacggc tgttggcctt cgagatagac
    24901 cagaccaaat tctttggcgt aatctgccac tcgaccatcg cggctggctt tgatttcgat
    24961 aagacgtgcc agtttctctt tcgtgaatcc gctttcatca actacagtgc cgctggagtc
    25021 tgacgcagtg atcacacgag caccaaattc catcgctttt tcgatagcgt actgggcgac
    25081 gttgccggag ccagaaacgg aaacgctcat cccttcaaaa cccataccgt ggcgttttaa
    25141 cattgcttct gtgaaataaa ccagaccgta gccggtagct tccgggcgaa taagactacc
    25201 gccaaatgaa aggcccttac cggtgaagac gcaggcggta ttgttggaga gctttttcat
    25261 catccccgcc ataaagccga cttcacgacc accaacgccg atatcacctg ccggaacgtc
    25321 ggtatccgcg cccaggtggc gatacagttc agtcatcagc gtctggcaaa aacgcatcac
    25381 tttaccttcg ctttttcctt tcggatcgaa atcgctgccg cctttaccac cgcccatcgg
    25441 cagggtagtc agggcatttt tgaaggtttg ttcaaagccg aggaatttga gaatggaaag
    25501 gttaactgac ggatggaagc gcataccgcc tttgtacggg ccgatggcag agctgaactg
    25561 cacacgccat gcacggttga cctgtatctg gttgcgatca tcaacccata ccacgcgaaa
    25621 ctggatcacg cgctccggtt caaccagacg ctccagtaat gacatctggc gatattttgg
    25681 attttgttca agaaaaggcc agagtgtggt cattacttca cgaacggctt gcgcgaactc
    25741 ggtttgattc gggtcgcgct tttggacatg gttgaggaat gactccagag aatatgtctg
    25801 atccatagat ataaaaacct tatatattaa tacgattgtg cttatgttgt ttatgtcatg
    25861 tgcttttgca gttttcgact ataccatcaa gaatgtgacg gtaagcaaga aaaaaatgac
    25921 ccaggaaagc aaattaataa cgagagtaat ctcataacca aatgcgataa ctcagacgct
    25981 aaagttttgc cacccggcta ccgttatcag tagcagagac aataacgtta aggagttccc
    26041 tgtgagtcga gcattgttcg ccgtagtatt agcatttcca ttaattgcac tggctaaccc
    26101 gcattatcgt ccggacgtgg aggtaaacgt accgccagaa gtgttcagtt caggcgggca
    26161 gtcagcgcag ccctgtactc agtgctgcgt ttatcaggat caaaattatt ctgaaggggc
    26221 ggtaattaaa gccgaaggaa tactgctgca atgccagcgt gatgacaaaa cccttagcac
    26281 taatccgctg ctctggcggc gcgtaaagcc ataaacgcct ctaataatgg aatgtcagca
    26341 ggggccagcg gatattgcag cgcctcttca ggtgagcacc agaccagcgc ctgatgttca
    26401 tgtgcctgta acgtcccgtg gaagtcgggt acgtgccagg catgaagatg gataatccgg
    26461 cccgaaactt ctcgctgatg gctggcaaca tactcaccca cagttgcttc gatgtccaat
    26521 tcttcgttta actcacgcac cagcacctgc tgctggcttt catccagctc gactttacct
    26581 ccggcaaact cccataatcc cgcctgatcg ctctgggcgg ggcgttgcgc gagtaaaatt
    26641 ttgccatcac gttcaatgat ggcggcaaca acttcaatca ttttcataaa ttacacacgg
    26701 gcagataagc gaaaaatgcc ataatatccg cactgttgct caatcagaaa ggctttatat
    26761 ttaaggagtg cctgtgctag accgccatct tcatccccgg attaaaccgt tgttgcatca
    26821 gtgcgtgcgg gttcttgata aaccgggcat tacgccagat ggtttaacat tagtgggatt
    26881 tgccatcggc gtgctggctc tgccgtttct ggcgctgggc tggtatctgg cggcgctagt
    26941 cgtcattttg ttgaacaggc tgcttgatgg tctggacggt gcgctggcgc ggcgcagagg
    27001 gcttaccgac gcgggcggct ttcttgatat ttctctcgat tttctctttt acgcgctggt
    27061 gccgtttggc tttattcttg ctgcaccgga gcaaaatgcg ctggcaggcg gctggttgtt
    27121 gtttgcgttt atcggcacgg gcagcagttt tctcgccttt gctgcactgg cagcaaaaca
    27181 tcagattgat aaccccggtt atgcacataa gtcgttttac tatctggggg gattaaccga
    27241 aggcacagaa acgatcttac tgtttgtgct gggatgttta ttccctgcat ggtttgcgtg
    27301 gtttgcgtgg atcttcggtg cgttgtgctg gatgacgacc tttacgcggg tgtggagtgg
    27361 gtatctgacg ctgaagtcgc tccagcgtca gtaattgcaa tgttatttgc tactgcccgg
    27421 gccgcgttca ccggttgcaa ccggattttt tggatcgctg ctccattcgt accagccgcc
    27481 gtcatacacg gaaacattct tccaacccat ggcgcgtgcg tacataaagg tttcggacgc
    27541 gcgccagccg gtgccgcagt agaatgaaac ttgctgatct ggtttgatat tccatgcttt
    27601 ccacatagcg gtaatatcat cggcgctacg catggtgcca tccgggttat agaaatcttc
    27661 catatgcgtc gagtcgctac cagcgtgtcc ccaacgtgct ccggctattt caccttttgg
    27721 tttaatatag ctgtaaccgc tggtcgtacc gataaattct ggccacgaac gaatgctcac
    27781 cagcgatgca tcctggcgat gcagcagtcc acgggcctgt tccatatcga gcatcaactg
    27841 cggttgtgcc gggatcttca cgccgaaatc cggttccgct ttcactttcg gtggcgttcc
    27901 tcgctcaaca ggcagtcccg cgtcggacca ggtttgccag ccgccatcca gcagacgcac
    27961 atctttcacg cctgcgtaga gcataatttg cgccacacgc gctccagcgt atacgtcacg
    28021 cccgtacaga atgaccgtgg tgtcatggcg aatgccgtgt tttgccagca tcgcttttag
    28081 ttgttcatca gaaactttgt tccacagcgg ttcgctttcc acttcgttgg tatcgatgta
    28141 gtcagcgcca ggaatatggc tgataaggta aagcttagga gcgccctagg ccgcttcaat
    28201 gactttccag tcaccgacag gtttcgccgt aacctcttta ccttgttgca ggtcatgcag
    28261 ccattgcgga taaaccagct gctcaaagtg cggcagtttt tgtagacggg aaggttcgct
    28321 tagcgcgtca ctcagaatgg agatatgcgt aaaacctgct ttttgcagtc gcgttttgac
    28381 ggcgtcgaca tctttgccat taccgtacag cgccaccggg gcatcggctt tcaggttatg
    28441 ttgcttgatc cacgcgttga gctgttcggt gctcattttg tcgagccagc tagcagagag
    28501 gtttaaggcg gcaggttcat gaccagaagg gccatttaag gtttgtggcc agccgttata
    28561 aaacgcgctg gggcgagtat ctatcgcttt gccattttgt tgctgaagtt ggtcaagtgt
    28621 aagaggcttc gccagttcag cggcccacga agaagcgcaa gccagcccta aagccattgc
    28681 cagcgcggtc atttgagaaa cacgtttcat cgaaaatccc gacattgtag ttttgagtgc
    28741 gccattctga actgacgcat tgagaaaaac tttgcgttat cgcagtttgt tgtaattttc
    28801 tgaccactgc gccatatcca gaacagaact atcagcagga acatcctgga gatcgtgcgt
    28861 tacctgaacg acggggatcg ccagggcgcg aacttcgctg aacacccact ggcgaaaatt
    28921 atcgcgcaga gccacatcaa gacggctgaa tggctcatcc aggagtaacg cttttggctg
    28981 ggcgagaagg gcgcgtagta gagcaacgcg cgctcgctga ccgccagaca aagtggcagg
    29041 atcctgatgg aaagctccgt caaggcctga acgctcaagt gcatcattca cggcattacg
    29101 tcgggcattc cccttaagtg tcgccggtag cgccagcagt aaattttgcc cgacgctgaa
    29161 ctggtcaaat aacagtgcat cctgaaaaag aatgccaatc tgacgctgtg cggtgggtag
    29221 catgtcaatc cgttgctcat tgagccatag ctcacctgta caagaaaact gtccggccag
    29281 tgcaccaatc atccatgaaa acagagtgga ttttccacag ccagacggcc ccattaacgt
    29341 gacgatgtca cctttatcaa ccgtaaagtt aacgtttgtc agcaagcggc tttctggtaa
    29401 acgtagcgaa acatttttca cgcagagcat tagcggattc cttgtctgac ataacctacc
    29461 cattttgcga ctaacgcggt cagggcaaaa ataataagcg gtaacaacag ttgccataac
    29521 gcctgggcgg cgagaatacc gttgctgccg ccgctgctta atgccaccgc ctcagtggtg
    29581 agcgtcggaa aacgccccgc gcccagccac agcgtcggca tatactgcgc aatactgact
    29641 gaaaatccca ccgcgaaggc agtcagcaca gggcgcaaca tgagtgggca tttcacgtaa
    29701 aagaagattt tggcccgcga ccagcccagt gtttgcgcaa tcaaaattaa ccgtgaatca
    29761 atgcgctgcc aggcgggttg caggataaac agcatccacg gcattaccca cagcagatgc
    29821 ccccagacca ccgccgtcca gcttccatcc aggttcagcc atagcgccag cgtgtactgg
    29881 cccgccacca gtggcagagc gggcaataga atgggcaacc atagccacaa ctggcgacgc
    29941 tgtgcccccc attccagcca cagcagtaac aggagcaaag cgatgaatgt tgctaccagc
    30001 cccattgtca gactgttgat gagtgcttca ctattgatcg tcgactgatc cgcgaggatc
    30061 gccagcagaa ccacacacag cacaccggtt aagggtaaaa aactcgccag cgtagtgcct
    30121 ggtaataaag gtgtggcagg cttgcgaatg ccatctacgc ggggaatagt gcgccgccag
    30181 ctgcgccaaa acgtaggcgg cgagtaacag catcagcagc agactggcga gtgcgccttt
    30241 cgtttgttga tccgcgtcgc cctgagttaa ccactgccag ctaattactg ccagcgtcgg
    30301 tggattacct ggcccaagaa taatcgccac atccacgacc gaaagcgacc aggcaacaat
    30361 cgccagcatc gccattgcca gcgaaggcgc gacggagggc aacagcagcc agttcaggca
    30421 ttgccagcgg ctgtagccca gcgaatccag cacaatgacc tgctgcaaca gccgtttttc
    30481 gctcaatact gccactaaga tccacagcag aaatgcgctt tctttcactg caagggtgag
    30541 gcccagaccg atccaaatcg gtccattggc ggagtgaaat acgggaaata gtcatatagc
    30601 agccctccgt cagcaaagag cagcagggcg ctggtggcaa aagccacatg gggaatggcg
    30661 agcagccacg gcagacgggc gcacatacgc tgccatttcg gcccaggcca tagcgccaca
    30721 atcaccagca gggcaatcaa caatgccccg accgccgcga tggttgtcga caccagcgtt
    30781 gccagtaatg cctgcggtaa ctgtggatcg gcaaataacg cctgccagcg tgtcaacgac
    30841 aacgcaggcg atatcagcgt gagagctgcc gggatcagcg gtgcataaat caccgccacc
    30901 atcgcccaca gcaaaaagat taatgcgtac cgtaacggcg taaccattct tgttccagtg
    30961 cattcaccca tcctgcgtgc ggttcagcca gtaccggcgg cagatcctgc ggcattcttg
    31021 attgcaatat ttcgcgctgc ccgtcaggca gtttttgcgg atcgagaaca gaaggatcgc
    31081 cccagacaac gggatctgct ttacgcagtt gcgcatcggg tgagagcagg aaattggcaa
    31141 ctaccttcgc cgcagcactg gcattcgcgt tggctggagt ggtgacgaaa tgcacgttgc
    31201 caatcatccc ctcgcgaaag ccaaaactgt aactgcttgc cggcaaatcg ccgctggcga
    31261 ttttttgctg cgcatgtgcg gggttaaagg tcagcgacag gcgcaatgtg ccggctttca
    31321 gcagagcatc catccgcgcg ggcgaaggcg ggaaatcctt tccttcgcgc cacaaatacg
    31381 gatgcagtgc atcaagatat tgctacaagg gcgcagtgac acgggcgaaa gtcgcatcgt
    31441 ccggcgcttc ttttaatgcg gcgggatctg gcgtcagcat aatcagcaac tgttcaagaa
    31501 acgccgtgcc ggtaaagtcc ggtgggcgcg gataagtaac cgtgccggga ttggctttag
    31561 caaactccag taaggcttgc ggcgtttgtg gtggctgtgg cgtaacatcg cggcgggcga
    31621 taaacgtcag ttgtgcgccg ccccagggcg attccgcacc ttgtgtcggc actgaaaaat
    31681 ctccccgcac cggcagctgt gtgtcgacat agcgccagtt gggcagagtc tccgcccagc
    31741 ccgtttgtaa taaat
//
LOCUS       DAJVRL010000039        31557 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVRL010000039 DAJVRL010000000
VERSION     DAJVRL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.039"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            699..1127
                     /gene="uspC"
                     /locus_tag="OQN02_001813"
     CDS             699..1127
                     /gene="uspC"
                     /locus_tag="OQN02_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCS1642634.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1134..2548)
                     /gene="otsA"
                     /locus_tag="OQN02_001814"
                     /pseudo
     CDS             complement(1134..2548)
                     /gene="otsA"
                     /locus_tag="OQN02_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2523..3323)
                     /gene="otsB"
                     /locus_tag="OQN02_001815"
     CDS             complement(2523..3323)
                     /gene="otsB"
                     /locus_tag="OQN02_001815"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCS1642635.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3490..4476)
                     /gene="araH"
                     /locus_tag="OQN02_001816"
     CDS             complement(3490..4476)
                     /gene="araH"
                     /locus_tag="OQN02_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1642636.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4491..6005)
                     /gene="araG"
                     /locus_tag="OQN02_001817"
     CDS             complement(4491..6005)
                     /gene="araG"
                     /locus_tag="OQN02_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1642637.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6075..7064)
                     /gene="araF"
                     /locus_tag="OQN02_001818"
                     /pseudo
     CDS             complement(6075..7064)
                     /gene="araF"
                     /locus_tag="OQN02_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7861..8364
                     /locus_tag="OQN02_001819"
     CDS             7861..8364
                     /locus_tag="OQN02_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1642638.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8444..8695)
                     /locus_tag="OQN02_001820"
     CDS             complement(8444..8695)
                     /locus_tag="OQN02_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1642639.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8810..8896)
                     /gene="azuC"
                     /locus_tag="OQN02_001821"
     CDS             complement(8810..8896)
                     /gene="azuC"
                     /locus_tag="OQN02_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1642640.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9159..9482
                     /locus_tag="OQN02_001822"
     CDS             9159..9482
                     /locus_tag="OQN02_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1642641.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9653..10150
                     /gene="ftnA"
                     /locus_tag="OQN02_001823"
     CDS             9653..10150
                     /gene="ftnA"
                     /locus_tag="OQN02_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1642642.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10187..10426)
                     /locus_tag="OQN02_001824"
     CDS             complement(10187..10426)
                     /locus_tag="OQN02_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1642643.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10616..11827
                     /gene="tyrP"
                     /locus_tag="OQN02_001825"
     CDS             10616..11827
                     /gene="tyrP"
                     /locus_tag="OQN02_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1642644.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11889..12554)
                     /gene="yecA"
                     /locus_tag="OQN02_001826"
     CDS             complement(11889..12554)
                     /gene="yecA"
                     /locus_tag="OQN02_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1642645.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12750..12836)
                     /locus_tag="OQN02_001827"
     tRNA            complement(12750..12836)
                     /locus_tag="OQN02_001827"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12800..12802),aa:Leu,seq:taa)
     gene            complement(12849..12922)
                     /locus_tag="OQN02_001828"
     tRNA            complement(12849..12922)
                     /locus_tag="OQN02_001828"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12888..12890),aa:Cys,seq:gca)
     gene            complement(12977..13052)
                     /locus_tag="OQN02_001829"
     tRNA            complement(12977..13052)
                     /locus_tag="OQN02_001829"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13017..13019),aa:Gly,seq:gcc)
     gene            complement(13204..13752)
                     /gene="pgsA"
                     /locus_tag="OQN02_001830"
     CDS             complement(13204..13752)
                     /gene="pgsA"
                     /locus_tag="OQN02_001830"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1642646.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13809..15641)
                     /gene="uvrC"
                     /locus_tag="OQN02_001831"
     CDS             complement(13809..15641)
                     /gene="uvrC"
                     /locus_tag="OQN02_001831"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1642647.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15638..16294)
                     /gene="uvrY"
                     /locus_tag="OQN02_001832"
     CDS             complement(15638..16294)
                     /gene="uvrY"
                     /locus_tag="OQN02_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1642648.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16590..16766
                     /locus_tag="OQN02_001833"
     CDS             16590..16766
                     /locus_tag="OQN02_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642649.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16753..16977
                     /locus_tag="OQN02_001834"
     CDS             16753..16977
                     /locus_tag="OQN02_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1642650.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17010..17630)
                     /gene="sdiA"
                     /locus_tag="OQN02_001835"
                     /pseudo
     CDS             complement(17010..17630)
                     /gene="sdiA"
                     /locus_tag="OQN02_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17860..18612)
                     /gene="yecC"
                     /locus_tag="OQN02_001836"
     CDS             complement(17860..18612)
                     /gene="yecC"
                     /locus_tag="OQN02_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1642651.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18609..19277)
                     /gene="tcyL"
                     /locus_tag="OQN02_001837"
     CDS             complement(18609..19277)
                     /gene="tcyL"
                     /locus_tag="OQN02_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1642652.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19292..20278)
                     /gene="dcyD"
                     /locus_tag="OQN02_001838"
     CDS             complement(19292..20278)
                     /gene="dcyD"
                     /locus_tag="OQN02_001838"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1642653.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20383..21183)
                     /gene="tcyJ"
                     /locus_tag="OQN02_001839"
     CDS             complement(20383..21183)
                     /gene="tcyJ"
                     /locus_tag="OQN02_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1642654.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21271..21822)
                     /gene="fliZ"
                     /locus_tag="OQN02_001840"
     CDS             complement(21271..21822)
                     /gene="fliZ"
                     /locus_tag="OQN02_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS1642655.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21868..22587)
                     /gene="fliA"
                     /locus_tag="OQN02_001841"
     CDS             complement(21868..22587)
                     /gene="fliA"
                     /locus_tag="OQN02_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS1642656.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22752..24404)
                     /gene="fliC"
                     /locus_tag="OQN02_001842"
     CDS             complement(22752..24404)
                     /gene="fliC"
                     /locus_tag="OQN02_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS1642657.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24670..26076
                     /gene="fliD"
                     /locus_tag="OQN02_001843"
     CDS             24670..26076
                     /gene="fliD"
                     /locus_tag="OQN02_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1642658.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26101..26511
                     /gene="fliS"
                     /locus_tag="OQN02_001844"
     CDS             26101..26511
                     /gene="fliS"
                     /locus_tag="OQN02_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1642659.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26511..26876
                     /gene="fliT"
                     /locus_tag="OQN02_001845"
     CDS             26511..26876
                     /gene="fliT"
                     /locus_tag="OQN02_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS1642660.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26954..28441
                     /gene="amyA"
                     /locus_tag="OQN02_001846"
     CDS             26954..28441
                     /gene="amyA"
                     /locus_tag="OQN02_001846"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1642661.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28475..28888)
                     /locus_tag="OQN02_001847"
     CDS             complement(28475..28888)
                     /locus_tag="OQN02_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1642662.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29075..30280
                     /gene="yedE"
                     /locus_tag="OQN02_001848"
     CDS             29075..30280
                     /gene="yedE"
                     /locus_tag="OQN02_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1642663.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30277..30510
                     /gene="yedF"
                     /locus_tag="OQN02_001849"
     CDS             30277..30510
                     /gene="yedF"
                     /locus_tag="OQN02_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1642664.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30617..31287
                     /locus_tag="OQN02_001850"
                     /pseudo
     CDS             30617..31287
                     /locus_tag="OQN02_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31327..>31557)
                     /locus_tag="OQN02_001851"
     CDS             complement(31327..>31557)
                     /locus_tag="OQN02_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1642665.1"
                     /translation="PAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNI
                     LAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7937 a         8057 c         7457 g         8106 t
ORIGIN      
        1 attagcatta gaatagttgc gataagctgc aataagcaga gccacctttt tggtttaata
       61 agtccttaca aatagaaggg ggtctttaca cttatctaag atttttccta aatcgacgca
      121 actgtactcg tcactacacg cacatacaac ggaggggggc tgcgattttc aataatgtgt
      181 gatgcagatc acacaaaaca ctcaattact taacataaat gtataagtca tacttttgtt
      241 ttgggtgtat ttcaatctgt taaaaagttt ttcgctacgc tagcaagcaa aaatgaaaca
      301 ggaataatcg aaatgggatg ttgcgcacgg ttaaaataac tcaccgtaaa taatcatctg
      361 ctataaataa tcactttcat gcaataccag ataagctatt tttaaacaga cacttaccgc
      421 acaacaaact aattaaatac aaagaaaggc aacaatcaac ttaatgttaa taaaatgttt
      481 ttcatatatt aaccacttgt ttgatgcggg gaatcagtac attattttct tataattaca
      541 gttgaaatca tatgatcacc gagagtgatt gattaattgt ctttactaat aatcagacta
      601 atatttacct gtttgactga gttgggattg cgtcgtttct ccattaggag taaagcttta
      661 atgtcacctg aagttcacag cagaataagg aacaggttat gagctatagc aatattcttg
      721 tcgctgttgc ggttacaccg gaaagtcagc aactgctggc aaaagcggta tctatcgcca
      781 ggccagtaaa gggacacatc agtttaatta ctctcgcttc caacccggaa atgtacaatc
      841 aattagccgc gccgatgctt gaagatttac gcagcgtgat gcaagaagaa acgcaaagct
      901 ttcttgataa gttaattcag gatgcggggt atcccgttga caagacgttt attgcctacg
      961 gtgagttaag cgaacatatt ctggaagtat gtcacaaaca tcatttcaat ttggtgattt
     1021 gcggtaatca caatcacagt ttcttttcgc gagcatcctg ctcggcgaaa agagtgattg
     1081 cctcaagtga ggtcgatgtg ctgttagttc cacttacggg agattaaccg ctcctacgca
     1141 agctttggaa aggtagcaac tttatcgcgc tgctggcttt ccgcgcttcg cggaactatc
     1201 tgctttaggt cgctaatgaa gcactcctgc cagtggttaa tatcgttttt cacgataacg
     1261 tccagcattt ctgcatgacg ggaaatacgt tccaccagcg acatagtcaa tgcacgatcc
     1321 agcgcagctg caacttcgtc acgatcgtag gggttaacaa ttaacgccga cgttaactcg
     1381 tttgccgctc ccgcaaattg cgaaagaaca agaacgcccg ggttggctgg gtcctgagca
     1441 gcaacatact cttttgctac caggttcatc ccgtcacgca gtggcgtcac taagcccacg
     1501 tcagagtagc ggaatatttt catcagtaat ttacggtcaa aatgctgatt caaataataa
     1561 agcggcgtcc agcctaattg cccgtattta ccattaattc gtccagcttc attttcgagc
     1621 tgatgacgaa tatcctggta ggcttgcaca tcaccacgcg acgttggtgc aatctgggta
     1681 taacgaattt taccatgatg ctgcggatat ttttccagca atgcctcata ggcgagaaaa
     1741 cgctccggca aacctttgga ataatccagc cgttcgacag aaaagatatt ttgtacgttt
     1801 ttcaattccg ctttaagttg cgccagtttt ggcggcagtg gcccggcagc ctgtttggct
     1861 atttctttcg gttcaatgcc gatcgggtag acttctgttc gaaatgcttt tccccaggct
     1921 gtatggcttt ttgcgctacg tgtcgtgacg cgggtcaggt tagaaaggca atccaggaac
     1981 gccagacgat cgttttctgt ctgtaaaccc agcaaatcat aatcacaaag ctgttcaagc
     2041 aaggtgtcat atgtcggcag cgcgttgaaa atttccggtg tcgggaaagg aatatgcaag
     2101 aaaaaaccaa tgcgattatt caccccacgt ttgcgtaatt catgcgcaaa gggcaacagg
     2161 tgataatcgt ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa tttatctgcc
     2221 agcaacgcat ttacgcgtag atagccgtcc caggcaggac gctgaaattg caccagatcg
     2281 agccgataat gaaaagcggg ccagagaacg gcattggaga attggttgta gtattcgtca
     2341 aggtcctgtt cgctgaggtt aaaagaggcc cacgtaatgt tacctttttt agcggctgat
     2401 cctcattccc tgtttcacca ctccagccaa accacagtcc gcctgcggct ttcagtgccc
     2461 ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg ctcgtctggt ggtgcaatcc
     2521 ggttagatac tacgactaaa cgactcatag tcatcactcc tgttattttc tcttttttgt
     2581 tgtaatgcgg cggttatcat ttcaagccag ctccagacat ccggcacacc cgccagtcgc
     2641 catgatgcct gagttgcacc tgtgccaatt tttactgaca ttccgcccag tcggttaacg
     2701 actgcgaagc cagattcatc ggttaaatca tcgcccagaa atacgggcgt tcgcccgata
     2761 aaaggagctt cctgcataaa ggctgcaatc gcttcacctt tactggtacc tctcggtttg
     2821 atctcgacaa cacactttcc ctgctgtaac gccatttgtg gccagatctg agtaatacgt
     2881 tgcgctaatg tcattaatgc gtcttcatgc tgcggagcct gacgataatg cagcgcaaaa
     2941 gccatccctt tcgcctccag ctccgcgccg ggatactgag cgatgaccgt atgcagttgc
     3001 acgctaatat cacgcgcaat cgcatccggc agatgaacga tatgtgtttt accattgatg
     3061 tcacggcgct ccgccccatg cacgcccgct aacgggaagc gataaggttt tgccagtgcg
     3121 tcaagctcca ccattgagcg ccctgatatc aatgccaatg caccatcact tgcggttgcc
     3181 agtagctgta gtccttgcag aatattgtca ggcacgacga cctgatcggg atgcggtttg
     3241 atttccgcca gcgttccatc aagatcaaaa aaccaggcat atttcgcgga tagttcaggg
     3301 gtttcggtta acggttctgt cacccggctc tcctccttct cttttcttat tgtgttgcgc
     3361 agaccgcgct ttacagacat gtaagtatag acagtgtgac ggaggtcgcc atttgcaacg
     3421 aagaacagcc aaccgttgtg gcggctggct gtggtgggaa aaaacgttaa attgttgtgg
     3481 aaaaaagcat cagacagtgc gcttcgcttt ttgcttgtaa cggtcgaaga tcaccgctgc
     3541 cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa gcaggttcat
     3601 ggcgttttcc acggtgccta aaattaagat acccgccacc acatatgaga tttttccgat
     3661 gccacctttc agagaaacgc cacctaaaac gcaggcggag ataacaataa gctcataacc
     3721 aatcgacgtc attggctgcc cactagtcat acgtgaagcc agaataattc cggctatcgc
     3781 agataccagg ccagagagaa caaagataat aattttggtg cgaacaaccg gtatacccgc
     3841 cagacgcgca gcctcttcgt tcccgccaat cgccagggta ttacgaccaa aggtggtttt
     3901 attcagcagc aaaccaaaga taatcagaca cgcgacggtg agccagattg gcgcaggcag
     3961 accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac cgaccgcttt
     4021 accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca gtgtcgtgat
     4081 cagagcattt attttcagtt tggcgataac aaagccattg accaggccac agagaacgcc
     4141 cagcaacaaa cccgctgcca cgccaatcca caggctttca gtcaggttga taaccaccgc
     4201 cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt caccggaagc
     4261 aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca ggcccaaccc
     4321 tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga taaagagcac
     4381 cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga atgacgactt
     4441 aggtgcgcca gaccccgatg tagaaacaga agacatcata ctctccttac tcaggcaact
     4501 gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc ctgctcgtgt
     4561 aacaattcac cggcgatttc gccttcccgc atcaccacaa tccggtcggc aacgccgagg
     4621 acttcaggta agtcgctgga ggcaaacagc accgccacgc cctgcgccgc cagcgcataa
     4681 attacgttat atatttcgtg cttagcgcca acatcaatgc cgcgcgtagg ttcatccagc
     4741 aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg ctgatttccg
     4801 cctgagagat tcatgatcag ttgctccgcg cccggcgttt tgatgttgag cgaacgaatg
     4861 tgctgatcgg cattgttttc ttcccaactg ttgttgatta cacaaccgcc gagcacatgt
     4921 ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat aatgccttcc
     4981 gctttgcgag cttccgggca gagcatcatg cctgcggcaa tggcgtggct cggtttacga
     5041 atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt cccgccaaac
     5101 aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc aacaatttca
     5161 ccactgcgaa ccgccagact tattggcgta cgcacgccta gtgctttcac agcatcaaga
     5221 cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc aatgtcgcgc
     5281 ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt aaaggttttg
     5341 acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat ttcttccata
     5401 cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat aacgcggaaa
     5461 agattgccga tttcgcgggc agaaagggag ctggttggct catcaaaggc gataatttta
     5521 gcgttacgcg ccagcgcttt ggcgatttca accatctgcc actgaccaat ggcgagatat
     5581 ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa acccgcctca
     5641 taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc gagatagatg
     5701 ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat cgccacaccc
     5761 gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat cactacagaa
     5821 cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt tcctgcgcca
     5881 ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat atccgtcagc
     5941 gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg ggtagactgt
     6001 tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat gcagagggga
     6061 atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc tttaaagttg
     6121 tcacgcgtga tcagtaccac gtctgtaact tcggtaaatt ttggcgtttc aacgtctttt
     6181 gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg gcttggcagc
     6241 agggaaccgt agaagccggt tgcctgtgct ttagacagtt cgcttactgc atccacaccg
     6301 ttaataccaa taccgatgat atcggctgct ttaaagccct gaccttcggt tgcacgtacg
     6361 ccgccaagta cggtgctgtc gttcataccg acgatcagcc aatgtttaac ttccggatgt
     6421 tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga tttggtaggt
     6481 acctgataaa tttgtttttc cgggaatcct gcagctttca gtgcgtccat agaacccgtt
     6541 gtacggcggc gggcggtatc cagttcgttg gcggtaatcg ccatcactgc gctttctttg
     6601 acatcccagc cacgtttctg catctcttta tacagttcct agccctgacg ttcgccaatt
     6661 ttagtcgccg ccatcatcac cagaggaacg gtatccattg gcttaccttt ggcgttaaca
     6721 aactggtcat ccactgcaat gactttcata tcgtagccac gcgctttcgc gacgatggca
     6781 gagccgagtt tcgggtccgg agtacaaata acgaaacctt ttgcgccact ggctgccagg
     6841 ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat aacctcaaac
     6901 cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca cggctcttcc
     6961 ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg tgacataacg
     7021 gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct ctccagcttt
     7081 agtgtcgttt tgtgtagtgc aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag
     7141 cattaccttt tatctaaaag ataagtgacc gtgttgacat agttttagcg agaaattaat
     7201 tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca taaatttagc
     7261 gggaaaagac ataaggaaaa gccaatttgt cagacaaatt gttgaatgca cagcagatta
     7321 atccataaga ttagcctgga aatccttgtt gtcttcggta cccattcggg atgtcttctt
     7381 tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt gtaataacca
     7441 caactcaaag atgtggaaaa tgcgcgtcat tcatttcgct attaattatc actgtgctca
     7501 ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa cgttttataa
     7561 aatcatcact tcatcatgaa ttcaaattca tcgattaata tcaacaagat acaaaaagca
     7621 ctatcattaa aattcattgc atttacattg atttcaccaa tgaaatgtaa aaatatataa
     7681 acttgatgat ttaagcattt tcttataacc gttcagacgt tattcttatt tcagatcatc
     7741 gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt ctgcaaacag
     7801 taattatggt gttttgattt atcttgcacc tctccacttc tggatataag gatattaggt
     7861 atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga gttttacgca
     7921 tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa cggcaccgcc
     7981 actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat gtttaacttt
     8041 atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttccagg tgaaaaactg
     8101 aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg ttcaagcact
     8161 ctggcgcagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt caatttcctg
     8221 cgcgatctgg aaaaagaaca gcagcatgat ggcctgttgc tgcaaaccat tcttgatgaa
     8281 gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca tgttctgaat
     8341 gtcgtgtcac accagctgca ttgatcatac atcggcgcta atacattgcg ccgatgaagt
     8401 ttttgagaaa ccgctgcctc atctgtttga agcagcggtt tttttaatgg gattcaccct
     8461 gtggggtaaa cttgagttca atcagcgcga tggctttttg gattgcccgc atggtgaccg
     8521 ggtctgcggc agcgggatga tgagtaaagt cgatattctt cagctgactg gacatttttt
     8581 cacgaacttc aacgggcgcg attacatcga gaacatccag aatttgtttg ataaccaact
     8641 ggcaagctac cacatcagaa accagttcct gatcggcatt cagcggctgg gacatcgtaa
     8701 actcctgata gcattttgaa agccgttata gtagcggctt cacatcttca gcgatagtca
     8761 catccaccgt catcaggaca caaaaaaacc tgccggagca ggttttttgt tatcggaaca
     8821 tattgcctgg cggtacgtct ttgaacgtct tgcaatagtt attgaacata cttttcagga
     8881 ttttgcgcag tttcatcgcg gcactccgac catttgttat acaggtgtta ttgtctttgc
     8941 gcttataata tgacaaccat cacaaaaatc aatctttatg tgatacaaat cacataaata
     9001 cccctttaat gttataaaaa tgataatcaa aaaacagccc ccctatttct gacacctaca
     9061 gatggcaaga aatagcgcct gccaggcgtc ttttccggcc attgtcgcag cgctgtaacg
     9121 cgtaaaatag tgctttctct tactcttctg gctggaccat gagacttctg attctgactc
     9181 tttcattaat aacgctggcg gggtgtacgg ttactcgcca ggcccatgtg agcgaagttg
     9241 acgcggcaac aggtattgta cggttggttt atgaccaggc ttttttgcag catgcccata
     9301 ccgatcgtta tgtcagtcgc gggatcgccg atcgcgcatg ccagcaggaa ggctataccc
     9361 acgcgatccc ctttggtcag ccagtaggca actgtagcct ttttgcgggt tccttatgtt
     9421 tgaatacgga attcactttg tcatatcagt gccatcattc cgccttccct gtctttctct
     9481 aaatatttta atcagcgagg ggatcttcgc tgattaaaga aatagcggta atgcgtttta
     9541 ttcccattcg tatttttaat aattgaagtt tatattttac cttttgcaaa taataaaata
     9601 acaaattata gtgacgccac agcaacaaat ataacctttg tggagcacta tcatgctgaa
     9661 accagaaatg attgaaaaac ttaatgagca gatgaacctg gaactgtact cttcactgct
     9721 ttatcagcaa atgagcgcct ggtgcagcta tcataccttc gaaggtgctg ccgcgttcct
     9781 gcgccgtcac gcccaggaag agatgacgca tatgcagcgt ctgtttgatt acctgactga
     9841 taccggcaat ttaccgcgta ttaataccgt tgaatctccg tttgctgaat attcctcact
     9901 tgatgaatta ttccaggaaa cctataaaca cgaacaatta atcacccaga aaattaacga
     9961 actggctcat gctgcaatga ccaatcagga ctacccaaca tttaatttcc tgcagtggta
    10021 tgtttctgag cagcatgaag aagagaaact gttcaaatcg attattgata aattaagcct
    10081 ggcaggcaaa agcggcgaag gtctgtattt tatcgacaaa gaactctcta ccctcgacac
    10141 acaaaactaa tgctctccgg cggcaggttt ctgccgccgt ttcaaatcag tgacggcaaa
    10201 tcttactctg atcggtcgaa aaaccttctt ctgaaggtat aaatttgcct tttgctgcca
    10261 gaaacgctac cagctctccc gctgtcaccc cttctgccga gcacgtgtga aaacgtgcct
    10321 gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat tgctcgcctg
    10381 attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt cctcctcaaa
    10441 aaaagcctag cgtagcgatt accgcttatg aagactttgc gccagcgcag gactgaatgc
    10501 ttttattgta catttatatt tacacaatat gtaacgtcgg tttgacgaag cagccgttat
    10561 gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag aaagcgtgaa
    10621 aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg caggcatgct
    10681 ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct tgttgattgg
    10741 gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc agcatgttcc
    10801 ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg gtcaatggct
    10861 gacgggcttc agtatgatgt tcttaatgta tgctctgact gcagcataca tcagcggtgc
    10921 cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt cggcaaccgc
    10981 tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt tgtgttggaa catcgctggt
    11041 cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg tgatgctggt
    11101 attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc aacaggggct
    11161 ggcactgtct gcaatcccgg tgatttttac gtcgtttggt tttcacggta gcgtgccgag
    11221 tattgtcagc tatatggatg gcaacattcg taagctacgc tgggtgttta taatcggtag
    11281 tgccatcccc ctggtggcat atattttctg gcaggtggcg acgcttggca gcattgattc
    11341 aacaaccttt atgggattgc tggctaatca tgctggatta aacgggctgt tacaggcgtt
    11401 acgcgaaatg gtggcctctc cgcatgttga gctggcggtg catttatttg ctgatttagc
    11461 cctcgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg ctgatttgtt
    11521 tcagcgttca aataccgttg gtggacgatt gcaaactggt gcaattacct ttctgccgcc
    11581 gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt acgccggtgt
    11641 ggcgctggcg gtactggcat tgattatccc ttcactattg acctggcaaa gcagaaagca
    11701 caatcctcag gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg tggtgtttct
    11761 ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcaggcttgt taccagaagt
    11821 ggggtgatca gatagcctca aattccttat tgggtgccag aattaacgct gacacccaat
    11881 gtgacctctt aatgcaggca gcactgctta aatttcttac cactgccgca cggacaagga
    11941 tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt ttcctgcgga
    12001 tgcgccatcc agtatgcatg taaatcaagc gccgccagtc gaatggcatc tacgctctct
    12061 tcaaacgctt ctggcgacat tttctctacc cgctcgaagt tttcctcagt accgtgcagc
    12121 gcaatcgcct ccagcgctgg ttttaaagaa tcgggcaacg ttgaccagtc agaaagtgcc
    12181 acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct gccatcaact
    12241 tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg ctctgcggta
    12301 tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc tgacgcccaa
    12361 cgcggcacat agtcagcccc accccacacg gcaaccagcc actgttctgg ttcaatctct
    12421 tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc aaggatggcg
    12481 tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact ttcgtttaac
    12541 ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct atcttacatg
    12601 ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa ttaaggaggg
    12661 tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg tggtttcacg acactgctta
    12721 ttactttgat tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca
    12781 gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat ccgggctcgg
    12841 gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc aatccgctac
    12901 ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat ccgcttgacg
    12961 ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgagacccc gaccttggca
    13021 aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt taatcacttg
    13081 attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct acttacctgg
    13141 cgcgatgagt caacgatatt tttcaccact tttgatcgtt tgctgaaaat tacgccgaaa
    13201 cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag
    13261 agtcagtacc gcagccacaa agaaaagtgc aataccgacg tactcaaccc aaatgttcgg
    13321 acgccacagc agccaggcca acgccaccat ctgggcagtg gttttcactt tcccaatcca
    13381 ggagacggcc acgctactgc gtttacccaa ctccgccatc cattcgcgta gcgcagaaat
    13441 aataatttca cgggcgatca tcgttgccgc cggtaatgtc acccaccagc tgtgataatg
    13501 ctcggttacc agcaccatgg cgatagccac gagaacttta tctgccacag ggtcaaggaa
    13561 agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga accagtcagt
    13621 caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga caggcagata
    13681 aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca acgtagggat
    13741 attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc tatgttgcta
    13801 cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg cgaaataccc
    13861 ggcacttttg caatttcctc gacgctggcg ttacgtaaac cttgcaaacc gcccatatat
    13921 ttcaacaaca tttgccgacg ttttggcccg acgccttcaa tggtttccag ggaactggta
    13981 tttttgacct tcgcccgttt tttacggtgc ccgccaatcg cgtgatcatg tgattcatcg
    14041 cgaatatgct ggataacatg cagcgcgggt gaatctggcg gcaaactaaa tccctcacct
    14101 tccggctcaa agaacagcgt ttccagtcca gccttacgat ctgctccttt ggcaacgcca
    14161 agtagcagcg gatgattttt atcccatgag acatccagtt cggcgaagac atttttcgcc
    14221 tgcgcaagct ggcctttgcc gccgtcgata aggatcacat ccgggatctt actgtcgtca
    14281 atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc atcgcccggc
    14341 gtgatgcctg taatgttata gcgccgatac tccgcacgca gcgggccgtt agcatcaaac
    14401 accacacagg aagcgacggt ttgttcgccc atggtatggc tgatgtcaaa gcactccatc
    14461 cgcttcactt ccggcaattt caacacgctg gcaagcgcgg tcagtcgctg gtgaacggta
    14521 gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcaga
    14581 taacgcgccc tatcgccgcg aggtttggtt tgaacattaa tcttgcgtcc cgccagttct
    14641 gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag gatctcaccc
    14701 ggtaaggtgc gcatctggct gccttgtaaa tagaactgac ctacgaaggt ttctaccacc
    14761 tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc gagcactttg
    14821 ccctgacgaa tgaacaatac gtggacacaa gccatgcccg catcgaacgc cacaccaata
    14881 acgtcaaggt cgtcgccggt attggaaaca aactgttttt cggtgacgcg tcgcaccgcc
    14941 tgaatttggt cgcgaataca tgccgcttct tcaaactcca gattctggct ggcagtttcc
    15001 atacggctaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa caggcgcaca
    15061 tactcgacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca cggtcccaga
    15121 cagcgcccta tctggtattg cagacacgga cgcgagcgat tgcgataaac actattttcg
    15181 cactggcgaa tggggaaaat cttttgcagt agcgccagtg tttcacgtac ggcatagcca
    15241 ttcgggaacg ggccgaaata ttcacctttg gcatgctttg caccacgatg catcgccaga
    15301 cgcgggtggg tatcgccact caggaagata aaaggataag atttatcatc gcgtagcaaa
    15361 acgttgtaac gcggctgata gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt
    15421 tctgtatgcg taaccgttac atcaatttgc tggatctggg cgaccagcgc ttcggttttg
    15481 cgcgaagcga ggttgctgcg gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg
    15541 ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg ctggctggtt
    15601 acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat aatgtctccg
    15661 cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca tgaatgttta
    15721 gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga ttgagctgtt
    15781 ctgagatctc attgaccttc tggcccttag tgatcatcag cataatctgc aattcacgtt
    15841 cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt aacgccattt
    15901 gttgagcgat atcagaagca atgtaacgct gccctgaata gacagaacga atcgcactca
    15961 cgacttcctg cggagccgcg cctttgctga ggtagccagc agcacctgcc tgcatgactt
    16021 tcgctggtaa agggttttct gtatggaccg taagcatgat gattttaaca tcagctgtgg
    16081 aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc atgtccatta
    16141 gcaccacgtc aacggcattt gtccggcacc acttaacggc gtcttcaccg cacgatgcct
    16201 ctccggcgac ttttataccc ttaatatctt ccagaatgcg tcgtatccct gcgcgcacca
    16261 gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa atagggataa
    16321 cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt gaaacgttaa
    16381 aaatggtggg tattcgattt ttctctcgtt tttgtcaatt cgattgtcca cagttatggt
    16441 cacgcccgtc accaacggct taccggatcg tttcacagta agccctacga tgaaatgtga
    16501 caaaaattga ctttattcag caaaaatgaa aatcagcccg ctgaatatgt aacattaatt
    16561 aacccgaaca atacgggcaa aaaacattca tgataaaaat atttattggt cattatatta
    16621 acgtatttta tagcactgcc gatatcacgc tcaaaaaaca accactgcta tttttagcaa
    16681 agcttatggt atactccgcc gccttaacat ttttcaccac aaattttcat tgcaacatga
    16741 cgaggaaaat aaatgagtac gcctgatttt tctactgccg agaataatca agaactggca
    16801 aatgaagtct cctgcctgaa agcgatgctg acgctgatgc tgcaggcgat gggacaagct
    16861 gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg cgctgatcga agacgaaacc
    16921 caggctgcag tattttccaa aacggttaag caaattaaac aagcctaccg tcagtaatat
    16981 aaaaccggct gagagcgtgc ctttcagccg gttattgaca aaatcatcgc tatctctgct
    17041 gatgtcttcc cttccgccgt ccatttcaga atttcttttt cgcgcttgct gaaattcatc
    17101 tctggcgtca tcactatttc atcatttaaa cgcatcagag ccatcagact ttcgcgcacc
    17161 agtaactgca tttttaattg cagttcatca ctaagaatgg gtatttcgcg cgtgctgcaa
    17221 cgggaaaagg acaaaaagcc cagcgcccga tttggcagca ttaaatactg agtgacaccg
    17281 cggcgtaaac catgtgcgtg cgcggcttcc cataatggct gtgcttcgct gaataagtca
    17341 tcattccaca ttaaatggcc ctgactaaag ttttcagggt tcagcacagg atcaattgcg
    17401 agaaaatttt ttgcctgata ataactaacc cacgactcag ggtaattggt gtagaaagcc
    17461 actttaggtc gcgtgaatgg taccgggtgg cgaacacata acgaatagta atcgtactcc
    17521 agctgctgag cctgaagctc tatttcatgg taggcctctt ctgcggcctc catcctctga
    17581 aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg tatcctgcat agtaaaccgc
    17641 aacgcccctg agagtgagta tcatttataa tgataattca aactatcaca taaataagat
    17701 ttatatataa tttatattat tcaggcaatg aattactttt gcaagccatc gcattctctt
    17761 atgttattaa tgagttatgc tgatttgtta agcagtttta tcacgcttga aatggcgtcc
    17821 cgccccgaca ggtgaatcgt cggggctgat tttttcttat tattgcagca gaagcttctc
    17881 gaggaactgg cgggtgcgag gctgctgggg atcggcaaat aacgcttttg ctgccccctg
    17941 ctcgactatc cgcccctggt ccataaagat cgcccggtcc gcaacatccc gggcaaagct
    18001 catttcgtgt gtcacaatca ccatcgtgcg cttttcctgc gccagctggc ggatggtgtt
    18061 caggacttcg cccaccagct ctggatccag cgccgacgtt ggctcgtcaa acaaaatcac
    18121 ctcaggacgc attgccagtg cacgcgcaat cgcaacacgc tgctgttgac cgccagacaa
    18181 acgacgtgga tagctggttt ctttaccggc cagcccaact ttcgccagca gctcgcgagc
    18241 gcgcgccgtg gcctcttctt tcggttcgcc tttgacgatc accggccctt caataatgtt
    18301 ttccagcacc gtacgatgcg gaaacaaatt aaagttctgg aagacaaacc cgacgtgctg
    18361 acgcaactgg cgaatcagcg atttttgctg acttaatgaa cgtgcagtat caatagtgat
    18421 atcgccaacg gtgatcgttc ccgcttccgg ttgttccagc agatttatgc tacgtagcaa
    18481 tgtggttttg ccggaaccac tcggaccgat aattgccacc acttcgccag gctttacctc
    18541 gaggtcgata ccgtgcagca ccgtctgacc gtggaatttt ttcaccaggt tcttaacttc
    18601 aatggcactc attttggttc tctctcctgg cgattaagtt gattctcaaa atggttctgc
    18661 aacgtcgata acacagtcgc catgatccag tagatgagcg aagccgccag gtacatggtg
    18721 aacacctcca gcgtgcgcga ggtaatcaac tgcgcctgac ggaacagctc cggcacctgg
    18781 attgtcgcgg ccagcgacgt atcttttacc agactgataa agctgttcga cagcggcggc
    18841 aacgccaccc gcgccgcctg tggcaaaatc gcgcgacgca tggtctgcca cggcgtcata
    18901 ccaatactgg cagcggcttc ccactgacct ttatcaatag aagaaatggc ggcgcgcagc
    18961 gtttcggcgg cataggcggc agtgttcagc gacagaccaa tcattgctga cggaatggga
    19021 tctaattcga taccaaactg cggcaggccg taatagatca taaacagttg tgcgatgagc
    19081 ggcgtaccac gaaaaataga gatataaaaa cgcgccagcc agcgcacggg ccagattggc
    19141 gacaggcgca tcagcgcgag aataaacccg agtagtaagc caaaaaacat gccgccgata
    19201 ctgagttgca gggtataccc agcccctttc aacaggaacg gcaaggaatc aataaccagt
    19261 tgtatacttt cttgcatgag cgttcccgga gctaaacgtg gggatgatag gcgaacagcg
    19321 caggcgcgcc gccggtatga ataaacagaa tcggcccttc atctttgaag cgtttctgac
    19381 tgataccgtc aatcagcccc gccatcgctt ttccggtata cacaggatca agcagaatgc
    19441 cttccagacg cgccagcaat ttcactgctt ccatgccttc gtcgttcggc acgccgtagc
    19501 caggtgcaaa atagtcatcc cagagtaaaa tttccgctga tgcggtcagc tccagttctt
    19561 tcgcaatcgc ctgttgtagg ttaaccactt tcggcaattg atcggcaacg gaacgcgaca
    19621 cggtcacgcc aatcagttcg ctttcaggca tcaggtgttc cagcccaaca gccagtccgg
    19681 cgtgagttcc ggcactgccc gatgcgacta ccaccgacga aatattaacc gccccttcac
    19741 actgttgcgc gatttccagc gcactctcca cataacctag cgcgccaaga gcattagaac
    19801 cgccaaccgg aatgacatac gggcgaaagc cttgtgcttc gactcgcgtc gccagttctt
    19861 ccagttgggc attgggatcg gtcagtgcgt cgcacatttc aatctgggta ttgaacagat
    19921 ccaacaacaa acgattgccg ttggttaaat agttttctgc ggttgtgcca ataggatttt
    19981 ccagcagcgc cacgcagtgc agaccgagtt tcgccgcgac tgcggcagtc tggcgcacat
    20041 ggttagactg gatcgcgccg gcagtaatca gcgtatcggc accttcgcgc agggcatctg
    20101 ccgcgagaaa ttccagctta cgtaatttat tgccgcccat tgccatgggg gtgacctcat
    20161 cccgtttgat gaaaatttcc cgtccaagat aatcagaaaa gcgcggcaga tattcgagcg
    20221 gcgttggcgc gccgataaac tccaaccgtg gaaaacgggt taaattatgc agtggcataa
    20281 cagcctccga tgtgtgttgt tgtgattttc ttattatgca cgctgaaaac gcgtaaataa
    20341 aaaaggcgct agtgaaagcg cccttttttg tcattatgct gattatttgg tcacatcagc
    20401 accaaaccat ttttcggaaa gagcttgcag agtgccgtct ttttgcattt ccgcaattgc
    20461 atcattcacc gctttcagca ggtcttcatt tcctttacgc agcgccacgc cagactcctg
    20521 acgggagaat gcttcaccgg ttactgccag cgtatcgttg gttttcttca ccagatccag
    20581 cgccgccaga cgatcaacga ggatcgcatc gatacgccct acgcgcagat cctgatattt
    20641 ggtcgggtca tcatcatagg tacgcacatc gacaccctga acattctgcc gcagccactc
    20701 ttcatagttg gtgcccagac cgacacccac ttttttgcct ttcagatcag cagccgtttt
    20761 aatggtgcct tcgttacctt ttttcaccag cgcctgaata ccggaaatgg tgtaaggggt
    20821 agagaaatcg tattttttct tgcgctcgtc agaaatggtg acctgattaa tcaccacatc
    20881 aatgcgttta gagtccagcg acgccagcat accgtcccat ttggtcggtt ttagtgacgc
    20941 ctcaacgcca agatgttttg ccagctgttg ggcaaattcc acttcaaaac cggttaattt
    21001 gccgtcatcc ccctgaaaac tgaacggcgg ataagtgcct tccagcccta ccagcagcgt
    21061 gccgctctct ttgactttat taagcagacc ttcatctgca aaacttttaa cgctcatgcc
    21121 cgcaaccagc gccacggcca tcacacccat caatgcctga cgtcccagat gtgctaattt
    21181 catattcacc ccgaatgttg ttatgtctgt ttgcagtgta gagccattgt ttgtaaacac
    21241 aaaaacaact ccgctacatc ttattcttat ttaatatata tcagaagcag gcaggctgga
    21301 ggatttctgc acaagtccgg tacaggtttg gcgctgatag tgttgatact tccgtaatgc
    21361 gatgcggtaa ttgttggtgc tggtggtggg cagccacggg gcgaggattt catcaaggaa
    21421 accgtcctgc agctgatcga gggaaatatt ttgctcatgt agatgattac ccaaacggcg
    21481 cagacgaacg acatattcac gcaccgtacc tgggctcatt tcagtttgct caaaaagaaa
    21541 tttcttaaag ccgataatat cgaaaaaatc actctgcgtt ttgcaatgta aatcaccgca
    21601 aaatcggcac aatgccgccc atgattgttg ttccgtttgc cagccttttt catcaagcag
    21661 cgtgtccagg cgggaaatct cgattttatt cactattttg ccgtcgcgaa ctaaggtaat
    21721 gcgatcgagt aatttacggc aatgcgcgca atgggtctgg ctgtgtttaa agtctttaag
    21781 atagcggctt aatggccgtc ttttcaggtg ctgcaccatc attaagaact cctggtagtc
    21841 aaagttaaag tgcggcattt actgacgtta taacttaccc agtttagtgc gtaaccgttt
    21901 aatggcctgg ctgtgtaact gactgacccg cgattccccg acctccagca ccgcgccaat
    21961 ctctttgaga ttcagctctt cctggtaata gagggttaat accagttttt cgcgctccgg
    22021 caacgtttcg atggcttcca tcacccgctg gcgcagatta ctgtccagca gttgttgtag
    22081 cgggttttct cgctgatgat catcagtaac cagttcgatg ctatcgccgt gctcttcacg
    22141 ccactcatcg taggagaaga gctggctgtt attggtgtcg agcagcattt gacgataatc
    22201 ggcaatatcg atccctaaac gttccgctac ctcagtttcc gtggcgttgc ggccaagttc
    22261 ctgctccagt tgccctattg cctgtgccac ttcacgcgcg ttgcgtcgca cgctgcgcgg
    22321 cacccagtca cggctgcgaa gttcatccag catagcgcca cggatacgct gcactgcgta
    22381 agttgtaaat gccgttcctt gtagggcgtc atagcgttcg acagcattaa gtaacccaat
    22441 gccgcccgcc tgtagcagat cgtcaagttc cacgctcgcg ggcagtcgaa cctgcaggcg
    22501 caatgcttcg tgacgcacca gcgggacata acgctgccac agcgagtgtt tatccattac
    22561 accttcagcg gtatagagtg aattcacgat aaacagccct gcgttatatg agttatcggc
    22621 atgattatcc gtttctgcag ggtttttaat cggacgatta gtgggtgaaa tgaggggtta
    22681 tttgggggtt acgcaaaaag ccgaatgtaa aaaaccccgc cggtggcggg gtttgagagg
    22741 cattcagacg attaaccctg cagcagagac agaacctgct gcggtacctg gttggctttt
    22801 gccagcacgg agttaccggc ctgctggata atctgcgctt tcgacatgtt ggacacttcg
    22861 gtcgcatagt cggcgtcctg aatacgggac tgcgcttcag acaggttggt agtggtgttg
    22921 ttcaggttgg tgactgcgga atccaaacgg ttttgcaccg cacccaggga ggagcggaat
    22981 ttgtcgatgg agctgatggc ttcatccaga gctttcaggg gatcggctgt cgtagctgct
    23041 tttgtttcag catctgtcgt caatttacca tcagcatctt tataaactgc cttaccgctg
    23101 ccgttagtca ctgaaccatc atcctgtagg taaaaatcta cagtatcaga tgtggtcaac
    23161 gcctcgttgt tatcaacata aaccgaatca acaccttttg ttacagcgcc agttgtgccg
    23221 ttgacgctat aacctgcagt agtgaaatca acattgtttc ccgaggttga tacagcttta
    23281 tcgaaagctg cctgatcaac ttttgcacct gttactgaat aagtaatagt gtccggcgta
    23341 gtactattac cggtaaagga gatagaggta ccgccaatgt caatggttgt cgctttggta
    23401 ttattggctg caagtgtgga cagactagcc tcagtcaaag aagcaccaga gccgtttttc
    23461 gttaagcgcc cagttgtatc aatgtagagt ttatctccat tgcttgccgt aattttacca
    23521 tcactgctga tctgaacatc ctgtgcagaa ccatcgatag taatagtgcc cttagcggta
    23581 tcaccaactc ccggggtcaa atatttctga acatcggcag ctgaagccgt tgtggtatca
    23641 aaagagtatg acttacttgc actgtcatac ttgtaattcg ttgcactagc cgccgttccg
    23701 aagccgttat taatggttgc ctgaacagta tcaccatcac taactccagc cagcaaatca
    23761 gacgctttag cagcatcgta acccgcactc acagtatatt tgttgccgac cactgttgca
    23821 ttagcagcta ccaagtcagc tttagatgca gcagtattag ccacagcccc gccaccattc
    23881 acattaaacc cattcagccc cagcgtatca gagtcaattt tcttcagatc aatagtgata
    23941 gtctggccgt cattcgcacc aacctgaatt ttcatcgaac cgtcttttgc cagtacgttc
    24001 acgccgttga actgggtctg accggatacg cggtcaattt cgtcgagacg ggatttgatt
    24061 tcgtcctgaa tggagtccag atccgaatca gagttagtcc cggtagaagc ctgaaccgtc
    24121 agctcacgga tacgctgtaa gttgttgttg atttcggaca gcgcgccttc agtggtctgt
    24181 gcaacagaaa taccgtcgtt ggcgttacgt gcagcctgag tcaggccttt aatgttagaa
    24241 gtaaaacggt tagcaatcgc ctgacccgcg gcgtcatcct tcgcgctgtt aatacgcaag
    24301 ccagaagaca gacgctcgat agaactcgac agcgcagact ggttcttgtt gatattattt
    24361 tgagtgatca gcgagaggct gttggtatta atgacttgtg ccatgattcg ttatcctata
    24421 ttgcaagtcg ttgattacgt tttgggtttc tacccgtcgg ctcaatcgcc gtcaaccctg
    24481 ttatcgtctg tcgtaaaaca acctttagaa tttttttcac aaacagccat tttttgttag
    24541 tcgacgaaat actcttttct ctgcccctta ttcccgctat taaaaaaaac aattaaacgt
    24601 aaactttgcg caattcaggc cgataacccc ggtattcgtt ttacgtgtcg aaagataaaa
    24661 ggaaatcgca tggcaagtat ttcatcgctg ggagtcgggt caggtctgga tttaagttca
    24721 atccttgata gcctcaccgc cgcgcaaaaa gcgacgctaa cccctatttc aaatcagcaa
    24781 tcgtcgttta ccgctaaact tagcgcctac ggtacgctga aaagcgcgct gacgactttc
    24841 cagaccgcca atactgcatt gtctaaagcc gatctttttt ccgccaccag caccaccagc
    24901 agcaccaccg cgttcagtgc caccaccgcg ggtaacgcca tcgccgggaa atacaccatc
    24961 agcgtcaccc atctggcgca ggcgcaaacc ctgaccacgc gcaccaccag agatgatacg
    25021 aaaacggcga tcgccaccag cgacagcaaa ctcaccattc aacaaggcga cgacaaagat
    25081 ccgattacca ttgatatcag cgcggctaac tcatcgttaa gcgggatccg tgatgccatc
    25141 aacaacgcaa aagcgggcgt aagtgcgagc atcattaacg tgggtaacgg tgaatatcgt
    25201 ctgtcagtca catcaaatga caccggcctt gataatgcga tgacactctc ggtcagcggt
    25261 gatgatgcgc tacaaagttt tatgggctat gacgccagtg ccagcagcaa cggtatggag
    25321 gtctcggtcg ccgcccagaa tgcgcagctg acggtcaaca acgtcgccat cgagaacagc
    25381 agcaacacta tcagcgacgc gctggaaaac atcaccctga acctgaacga tgtcaccacg
    25441 ggcaaccaga cgctaaccat cactcaggac acctccaaag cgcaaacggc gattaaagac
    25501 tgggtgaatg cctataactc gctaatagat accttcagca gcctgaccaa atacaccgcc
    25561 gtagatgcgg gagctgatag ccagagttct agcaatggcg cactgctcgg cgactccacg
    25621 ctgcggacga ttcagacgca gttgaagtcg atgctgagta ataccgtcag ttcttccagc
    25681 tataaaacgc tggcgcagat tggtatcacg accgatccca gcgatggcaa actggaactg
    25741 gatgccgaca aactcaccgc tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt
    25801 gttggcgatg gtaaaaaaac cggcatcaca accaccatcg gcagcaacct gaccagttgg
    25861 ctttcgacaa cgggcattat taaagccgct accgatggcg ttagtaaaac cctgaataag
    25921 ttaactaaag actacaacgc cgccagcgat cgcattgatg cgcaggtcgc gcgctacaaa
    25981 gaacaattta cccaactgga cgttttaatg acctcgttaa acagcaccag cagctactta
    26041 acgcagcagt tcgaaaacaa cagtaattcc aagtaatcat tattcatcgg gagacaggtc
    26101 atgtacgcgg caaaaggcac ccaggcctat gcacaaattg gcgtcgaaag caccgtaatg
    26161 agcgccagcc aacagcagtt ggtcaccatg ctatttgatg gagtgctgag cgcactggtt
    26221 agagcgcgac tgtttatgca ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa
    26281 gcgatcaaca tcattgagaa cggactgcgg gtgagtcttg atgaagagag caaagacgaa
    26341 ttaacccaaa acttaattgc tctttatagc tatatggtca ggcgtttgct gcaagccaat
    26401 ttacgcaacg atgtctccgc agtcgaagag gtggaagcat taatgcgtaa tattgccgat
    26461 gtctggaaag agtctttact ctccccttct tcgattcagg acccagtctg atgaacaatg
    26521 caccgcattt gtatttcgcc tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc
    26581 tggcaacgga agaacaatgg gacgaactca tcgctagcga aatggcgtat gtgaatgcgg
    26641 tgcaggagat cgcacacctg actgaagagg ttgccccgtc cactacgatg caggagcagc
    26701 tccgcccgat gctgcacctg attctcgaca acgaaagcaa ggtaaagcag ttattacaga
    26761 ttcggatgga tgaactggcg aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttaa
    26821 gtgcctatgg cgatcagggc ggctttgtgc tggctccgca ggataacctc ttttgaatct
    26881 taatgagccg atggctcgcg aataatccga ttacggctac gcttctaata ttccccttga
    26941 atggagtcga agaatgcgta atcccacgct gttacaatgt tttcactggt attacccgga
    27001 aggcggtaag ctctggcctg aactggccga gcgcgcagac ggttttaatg atattggtat
    27061 caatatggtc tggttgccgc ccgcctataa aggcgcatcg ggcggctatt cggtcggcta
    27121 cgactcctat gatttatttg atctaggtga gtttgatcag aaaggcagca tccctactaa
    27181 atatggtgat aaagcacaac tgctggccgc cattgacgcc ctgaaacgta atgacattgc
    27241 ggtgctgctg gacgtggtag tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg
    27301 cgtgcagcgt gtaaatgctg atgaccgtac gcaaattgac gaagaaatca ttgagtgtga
    27361 aggctggacg cgttacacct tccccgctcg tgccgggcaa tactcgcagt ttatctggga
    27421 tttcaaatgt tttagcggta tcgaccatat cgaacaccct gatgaagatg gcatttttaa
    27481 aattgttaac gactataccg gagaaggctg gaacgatcag gttgatgatg aactcggtaa
    27541 tttcgattat ctgatgggtg agaatatcga ttttcgcaat catgccgtga cggaagagat
    27601 taaatactgg gcgcgctggg tgatggaaca aacgcaatgc gacggctttc gtcttgatgc
    27661 ggtcaaacat attccggcat ggttttataa agagtggatc gaacacgtac aggaagttgc
    27721 gccaaagccg ctgtttattg tggctgaata ctggtcgcat gaagtcgata aactgcaaac
    27781 gtatattgat caggtggaag gcaaaaccat gctgtttgat gcgccgctgc agatgaaatt
    27841 ccatgaagca tcgcgcatgg gtcgcgacta cgacatgacg cagatcttca ctgggacgct
    27901 ggtggaagcc gatccttttc acgccgtaac cctcgttgcc aaccacgaca cccaaccgtt
    27961 gcaagccctc gaagcaccgg tcgaaccgtg gtttaaaccg ctggcgtatg ccttaatttt
    28021 gttgcgggaa aatggcgttc cttcggtgtt ctatccggac ctctacggtg cacattacga
    28081 agatgtcggt ggtgacgggc aaacctatcc gatagatatg ccaataatcg aacagcttga
    28141 tgagttaatt ctcgcccgtc agcgtttcgc ccacggtgta cagacattat ttttcgacca
    28201 tccgaactgc attgccttta gccgcagtgg caccgacgaa tatcccggct gcgtggtggt
    28261 catgtcgaac ggagatgatg gcgaaaaaac cattcatctg ggagagaatt acggcaataa
    28321 aacctggcgt gattttctgg ggaaccggca agagagcgta gtgacagacg aaaacggcga
    28381 agcgaccttc ttttgcaacg gcggcagcgt cagcgtgtgg gttatcgaag aggtgattta
    28441 aatccatccc cggcggcaag ccggggagat ttcattacgg cagtggcgtc ggtaacggcg
    28501 ctttatccag cgcggcggca cactctgccg tcgggcgatc cacacgctcc atcgtcatac
    28561 catcgtactc aatggtgtta ccgtcacgct caacctcgta cagctcacgt tttacggtaa
    28621 cgttagtgag atcatcagac atcaatgtca acttacccgg cacggcaatc ccccgctgcc
    28681 actgacggca atccaaggta tcgccctctt tagtgacaat caggctgcct atcgcttccg
    28741 gactgaccag cgcacgttgc ggccctttgg tctgccagta gcccgccagc cagtccggcg
    28801 cgggggtttt cacgacattg ttatagtttt cgacttcggc acagcccgcc agcaacatga
    28861 gcgcgcctgc aattgcgagt tttttcatca ttcgtcctgc atgagaagaa aagaggattg
    28921 tggcattaaa gctctgatgt cgccagcctt tccaggttca gagagaaatt tgatctaact
    28981 attatttttg tgaattatta ccccgaaaat aaatttgcat ggtgtatgat tcgcggctct
    29041 tgtttcaaac cttctgagtt cagaggctac attcatgtca tggcagcaat tcaaacacgc
    29101 ctggttgatt aaattctggg cacccatccc tgcggtcatc gcggcgggta ttctctccac
    29161 ttactatttt ggcattactg gcaccttttg ggctgtcacg ggtgaattta cccgttgggg
    29221 cggtcagctc ctgcagctgt ttggcgtcca tgctgaagag tggggttatt ttaaaattat
    29281 ccatctggaa ggatcgccat taacccgcat cgacggcatg atgatcctcg gtatgtttgg
    29341 cggctgcttt gctgctgcgc tgtgggccaa caatgtcaaa ctacgcatgc cacgcagccg
    29401 tatccgcatt atgcaggcca tcattggcgg cattatcgcc ggttttggcg cgcgtctggc
    29461 aatgggctgt aacctggcgg cgttctttac cggcattcct cagttctcgc tgcatgcctg
    29521 gttctttgcc atcgccactg ccattggttc atggtttggc gcgcgcttta ccctgctgcc
    29581 catcttccgt attcccgtga aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa
    29641 accggatcag gcgcggcgtc gttttcgtct cgggatgctg gtctttttcg gcatgctggg
    29701 ctgggcgctg ctcacggcga tgaaccaacc aaaactgggg ctggcaatgc tgtttggcgt
    29761 cggctttggt ttactgattg aacgtgcgca aatctgcttt acttcggcgt tccgcgatat
    29821 gtggatcacc ggacgtaccc atatggcgaa agcaatcatt atcggtatgg cggtgagtgc
    29881 catcgggatc ttcagttacg tacagttagg cgttgaacct aaaatcatgt gggcgggacc
    29941 aaatgcggta attggtggtt tactttttgg ttttggcatc gtgctggcag gcggctgcga
    30001 aaccggctgg atgtaccgcg cggtagaagg ccaggtgcac tactggtggg tcggtctggg
    30061 caacgtgatc ggctcaacga ttctggcgta ttactgggat gatttcgctc cggcgctggc
    30121 caccgactgg gacaaaatca acctgctgaa aacttttggt ccgatgggag gcctgctggt
    30181 gacatatttg ctgctgtttg ctgcgctgat gttgattatc ggctgggaaa aacgcttctt
    30241 cccccgtgag gcaccgcaga ctgctaagga gatcgcatga aaaatatcgt tcctgattac
    30301 cgccttgata tggtgggtga accctgccct tatccggcag tcgcaaccct tgaggcgatg
    30361 ccgcaattaa aaaaaggtga gatcctggaa gtggtgagcg actgtccgca gtcgatcaat
    30421 aatattccac tggatgcgcg taatcacggc tacacggtgc tggatattca gcaagacggg
    30481 ccgaccattc gttatttaat tcagaagtaa tccctcattc ccgctgtggc ccacagcggg
    30541 aacatcgtta cctctgacac atagatttta cccgccccct ctcctattct tttcagtatg
    30601 atctcaggag gttgctatgt gtggacgctt tgcccaatcc caaacgcgtg aggattacct
    30661 tgcgcttctc gcggaagata ttgaacgcga tattccctac gatcccgaac ccattggcag
    30721 atacaatgtc gcgccgggaa ccaaagttct tctgctcagt gaacgtgatg aacaccttca
    30781 tctggatccg gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa
    30841 cgcccgcgta gaaactgcct caaccagtcg catgtttaaa ccgctctggc aacatggtcg
    30901 ggcaatctgt tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaaaagcct
    30961 tattttatct atcgcgctga cggacaacct gtttttatgg ccgcgatagg tagcataccg
    31021 tttgagcgtg gtgacgaagc cgaaggattt ttgatcgtca ctgcggcggc agatcaaggt
    31081 ctggtagata ttcatgaccg acgcccgctg gttctgtcgc cagaagctgc acgggaatgg
    31141 atgcggcaag acattggtgg taaagaagcc tcagaaatcg ctaccagaag ctgtgtgcca
    31201 gcaaaccagt tcacctggca ccctgtgtcg cgcacggtgg gtaatgtcaa aaaccagggc
    31261 gcggagttaa ttcaacctgt ttgttgacat cctccacgcc ctgaatgacg aggacccctg
    31321 ctacgttcag gctgtcgcct gaatcatttc ggtgggttcc tgcttcaacg agcggcctga
    31381 ctgcaccatc tctccacagg caagaacggc gtgccccgcc gctaaaatgt tacgagctcc
    31441 atttacatcg gcgttcgctg tatatccaca tacctggcat ctgaatttac tttgtgacag
    31501 gcgattttct ttcgctgtat gaccacagca cgcgcaacgc tggcttgtgt acgctgg
//
LOCUS       DAJVRL010000040        31410 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVRL010000040 DAJVRL010000000
VERSION     DAJVRL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.040"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(437..643)
                     /locus_tag="OQN02_001852"
     CDS             complement(437..643)
                     /locus_tag="OQN02_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1642666.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            956..1045
                     /gene="kdpF"
                     /locus_tag="OQN02_001853"
     CDS             956..1045
                     /gene="kdpF"
                     /locus_tag="OQN02_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1642667.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1045..2718
                     /gene="kdpA"
                     /locus_tag="OQN02_001854"
     CDS             1045..2718
                     /gene="kdpA"
                     /locus_tag="OQN02_001854"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1642668.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2741..4788
                     /gene="kdpB"
                     /locus_tag="OQN02_001855"
                     /pseudo
     CDS             2741..4788
                     /gene="kdpB"
                     /locus_tag="OQN02_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4797..5369
                     /gene="kdpC"
                     /locus_tag="OQN02_001856"
     CDS             4797..5369
                     /gene="kdpC"
                     /locus_tag="OQN02_001856"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS1642669.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5362..8042
                     /gene="kdpD"
                     /locus_tag="OQN02_001857"
                     /pseudo
     CDS             5362..8042
                     /gene="kdpD"
                     /locus_tag="OQN02_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8039..8716
                     /gene="kdpE"
                     /locus_tag="OQN02_001858"
     CDS             8039..8716
                     /gene="kdpE"
                     /locus_tag="OQN02_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS1642670.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8991..9095
                     /locus_tag="OQN02_001859"
     CDS             8991..9095
                     /locus_tag="OQN02_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCS1642671.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9406..11603
                     /gene="speF"
                     /locus_tag="OQN02_001860"
                     /pseudo
     CDS             9406..11603
                     /gene="speF"
                     /locus_tag="OQN02_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11600..12919
                     /gene="potE"
                     /locus_tag="OQN02_001861"
     CDS             11600..12919
                     /gene="potE"
                     /locus_tag="OQN02_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS1642672.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12988..14628)
                     /gene="pgm"
                     /locus_tag="OQN02_001862"
     CDS             complement(12988..14628)
                     /gene="pgm"
                     /locus_tag="OQN02_001862"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1642673.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14654..15199)
                     /gene="seqA"
                     /locus_tag="OQN02_001863"
     CDS             complement(14654..15199)
                     /gene="seqA"
                     /locus_tag="OQN02_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1642674.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15384..16148
                     /gene="ybfF"
                     /locus_tag="OQN02_001864"
     CDS             15384..16148
                     /gene="ybfF"
                     /locus_tag="OQN02_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1642675.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16288..16581
                     /gene="ybfE"
                     /locus_tag="OQN02_001865"
     CDS             16288..16581
                     /gene="ybfE"
                     /locus_tag="OQN02_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1642676.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16721..17251
                     /gene="fldA"
                     /locus_tag="OQN02_001866"
     CDS             16721..17251
                     /gene="fldA"
                     /locus_tag="OQN02_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1642677.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17461..17547
                     /gene="uof"
                     /locus_tag="OQN02_001867"
     CDS             17461..17547
                     /gene="uof"
                     /locus_tag="OQN02_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1642678.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17540..17986
                     /gene="fur"
                     /locus_tag="OQN02_001868"
     CDS             17540..17986
                     /gene="fur"
                     /locus_tag="OQN02_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1642679.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18070..18396)
                     /locus_tag="OQN02_001869"
     CDS             complement(18070..18396)
                     /locus_tag="OQN02_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642680.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18433..19839)
                     /gene="chiP"
                     /locus_tag="OQN02_001870"
     CDS             complement(18433..19839)
                     /gene="chiP"
                     /locus_tag="OQN02_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCS1642681.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20418..22082)
                     /gene="glnS"
                     /locus_tag="OQN02_001871"
     CDS             complement(20418..22082)
                     /gene="glnS"
                     /locus_tag="OQN02_001871"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1642682.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22285..24231)
                     /gene="nagE"
                     /locus_tag="OQN02_001872"
     CDS             complement(22285..24231)
                     /gene="nagE"
                     /locus_tag="OQN02_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1642683.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24564..25364
                     /gene="nagB"
                     /locus_tag="OQN02_001873"
     CDS             24564..25364
                     /gene="nagB"
                     /locus_tag="OQN02_001873"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1642684.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25424..26572
                     /gene="nagA"
                     /locus_tag="OQN02_001874"
     CDS             25424..26572
                     /gene="nagA"
                     /locus_tag="OQN02_001874"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1642685.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26581..27801
                     /gene="nagC"
                     /locus_tag="OQN02_001875"
                     /gene_synonym="nagR"
     CDS             26581..27801
                     /gene="nagC"
                     /locus_tag="OQN02_001875"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1642686.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27849..28601
                     /gene="nagD"
                     /locus_tag="OQN02_001876"
     CDS             27849..28601
                     /gene="nagD"
                     /locus_tag="OQN02_001876"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS1642687.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28998..30662
                     /gene="asnB"
                     /locus_tag="OQN02_001877"
     CDS             28998..30662
                     /gene="asnB"
                     /locus_tag="OQN02_001877"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1642688.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31041..31117
                     /locus_tag="OQN02_001878"
     tRNA            31041..31117
                     /locus_tag="OQN02_001878"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31075..31077,aa:Met,seq:cat)
     gene            31126..31210
                     /locus_tag="OQN02_001879"
     tRNA            31126..31210
                     /locus_tag="OQN02_001879"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31160..31162,aa:Leu,seq:tag)
     gene            31234..31308
                     /locus_tag="OQN02_001880"
     tRNA            31234..31308
                     /locus_tag="OQN02_001880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31266..31268,aa:Gln,seq:ttg)
BASE COUNT         7485 a         7930 c         8181 g         7814 t
ORIGIN      
        1 aacataacat cttattggat tagatatgtt aacaattaat tattcttaat atatcaataa
       61 agtatatatt actctcttta ctatctgttt ttattgcggt agcgatacta ataacaaaat
      121 tcggaattta tttagatgct ggaattagaa aaagggatga cggctttctc accttaccct
      181 gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac tggagatttc tgatgatcta
      241 gtcgcttaga gcgacaatga aggtgaccgc atcctgggta gccctatgaa aggatactat
      301 tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg atggaaacgt cttccatgtt
      361 aattatcatg gaagacgttg atgttgaaca cctaaaccag ccccctgata cgaggctggc
      421 ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      481 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      541 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      601 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      661 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      721 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      781 tacattcaca ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat
      841 catggccttt gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct
      901 ttgcagccag aattctactc ttccggtatc acttttaggc cactggaggt gcactgtgag
      961 tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggttatt tggtttatgc
     1021 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
     1081 ttactggtat taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
     1141 gacattcctc ttccaggtac agcgggcgtt gaacgtatac tctttcgttt acctggcgtc
     1201 tctgaccatg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
     1261 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag
     1321 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1381 accaactggc aatcttatag cggtgaaacc acgttgagct atttcagcca gatggcaggc
     1441 ttaacggtac aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccttcatc
     1501 cgtgcgttta ctcgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1561 atcacgttat gggtgctagt ccctgtggcg ttgttgatcg cactgttttt tattcaacaa
     1621 ggtgcgcagc aaaactttct gccctatcag gcagtgaata ccgttgaagg agcgcaacag
     1681 ctgttaccca tgggaccagt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1741 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1801 ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa
     1861 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1921 atctgcgtag gcgtggttat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1981 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     2041 agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg cggtgattgc gatgcatgat
     2101 tcgtttaccg ctctcggcgg catggtgccg atgtggctaa tgcaaattgg tgaagtggtg
     2161 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     2221 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat agacgtacgc
     2281 gagatgaaac tgactgcact ggcgattctg gttactccaa cactggtact gatgggcgcg
     2341 gcgttggcga tgatgaccga cgccggacgt agcgccatgc ttaaccctag cccgcatggt
     2401 tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca acaacggcag cgcctttgcc
     2461 ggattaagcg ccaactctcc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2521 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2581 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2641 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2701 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2761 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2821 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2881 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2941 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     3001 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     3061 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     3121 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     3181 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     3241 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     3301 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3361 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3421 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3481 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3541 tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt
     3601 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3661 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3721 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3781 ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3841 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3901 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atattgaggc
     3961 taacggcggt cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaagg
     4021 agccacgctg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     4081 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaatggg cattaaaacg
     4141 gtgatgatta ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc
     4201 gatgattttc tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag
     4261 gcggaaggtc gtttggtagc gatgaccggc gacagcacca acgatgctac ggcgctggcg
     4321 caggcagatg tcgcggtggc gatgaactcc ggcacccagg cggctaaaga ggcgggcaat
     4381 atggtggatc tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag
     4441 atgctgatga cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac
     4501 ttcgccatta ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc
     4561 atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt
     4621 atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct
     4681 gccatgttgc gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt
     4741 ggtatcaaag tcattgattt actgctaacc gtttgtagtc tggtgtgagg tttaccatga
     4801 gtggattacg tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt
     4861 acccgctgct gaccaccgca ctggggcaat ggtggtatcc ctggcaggcc aacggttcgt
     4921 tgattcgtga aggtgatacg gtgcgcggtt cggcattaat cgggcaaaat tttaccggca
     4981 acggctattt tcatggtcgc ccgtcggcaa cagcagaaat gccctataat ccacaggctt
     5041 ctggcgggag caatctggca gtcagtaacc cggagctgga taaacagata gccgcacgcg
     5101 ttgccgcatt acgggccgct aacccggacg ccagcacgaa cgttccggtt gaactggtga
     5161 cggcgtcggc aagcggactg gacaataaca tcaccccgca agcggcggcc tggcagatcc
     5221 cacgcatcgc aaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat
     5281 acagccaaca accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca
     5341 atctggcact ggataagctt gatgaataac gaacccttac gtcccgaccc cgaccgtctg
     5401 ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag ttttcttcag tgcctgtgca
     5461 ggcgtcggga agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg
     5521 gatattgtgg ttggtgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa
     5581 gggctggctg ttctgcctct gaaacgccag gcgtaccgtg ggcggcatat cagcgagttt
     5641 gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac
     5701 agtaatgcgc caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa
     5761 gctggcattg atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg
     5821 gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc
     5881 gccgacgacg tggtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa
     5941 ggcaaagtct atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc
     6001 aatctgatcg ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa
     6061 atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc
     6121 cttttatgca tcggccataa caccggcagc gaaaactggt ccgcgcggcg gcgcggctga
     6181 cgtcacggct ggggagcgtc tggcacgcgg tgtatgttga aacccctgcc ctgcatcgct
     6241 taccggagaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     6301 cggagacggc aacactttct gatccagcgg aagagaaagc ggtagtgcgt tatgcccgtg
     6361 aacataatct cggcaagatt attctcggtc gcccagcctc gcgccgctgg tggcgtcggg
     6421 aaacatttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6481 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctca tttaaagaca
     6541 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct
     6601 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6661 ttggcgtggt ggtggcgcta ttttatggac gctggccttc ggtggttgcc accgtcatta
     6721 atgtggtgag tttcgatctc ttttttatcg ccccacgcgg cacgctcgcc gtctctgatg
     6781 tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg gctggtgatc gggaacctta
     6841 ctgctggcgt gcgttatcag gcgcgggtag cccgttaccg cgagcaacgc acacggcact
     6901 tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc gcaggacatc gccgccacca
     6961 gcgaacaatt tattgcctcc acgtttcatg cccgcagtca ggtgttgttg cccgatgaca
     7021 acggtaaatt gcagccgtta acacatccgc aaggagttac gccatgggac gatgccatcg
     7081 cgcagtggag ttttgataaa ggcctgcctg cgggcgcggg caccgacacg ttacccggtg
     7141 taccgtacca gattttgccg ctaaaaagcg gcgagaaaac ctacgggctg gtggtggtgg
     7201 agccggggaa tctgcgccag ttgatgatcc cggaacagca gcgcctgctg gagactttta
     7261 cgctgttagt cgccaatgcc cttgagcggc tgacgctaac cgccagcgaa gaacaggcgc
     7321 ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcagcgctt tcgcatgatt
     7381 tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat cttaacgctc gatctggcaa
     7441 gcgaaggatc accccacgcc cgccaggcca gcgagattcg tcagcatgtg ctgaacacta
     7501 cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc tttaatttga
     7561 agaaagagtg gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg ccggaaccgg
     7621 gtttatcgtc gcccatcaat ctttctctgc cagaaccgct gaccttaatc cacgttgacg
     7681 ggctactctt tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa tatgcgggtg
     7741 cgcaggccga aattggtatc gatgcccacg ttgagggcga aaatctacaa ctggatgtct
     7801 gggataacgg ccccggtctt ccgccaggcc aggagcagac gatatttgat aagtttgctc
     7861 gcgggaataa agagtcggcg gtgccgggtg tcgggcttgg gttggcaatt tgtcgggcga
     7921 ttgtggatgt acacggcggc accattaccg cgttcaaccg accggaaggt ggtgcctgtt
     7981 ttcgtgttac acttccccag caaaccgctc ctgaacttga agactttcat gaggatatgt
     8041 gacaaacgtt ctgattgttg aagatgaaca ggctattcgt cgctttctgc gcacggcgct
     8101 ggagggcgac gggatgcgcg tctttgaggc cgaaactctg caacgcggct tgctggaagc
     8161 ggcaacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg gtgatgggat
     8221 tgagtttatc cgcgacctgc gccagtggag cccggtgccg gtgattgtgc tttccgcacg
     8281 cagcgaagag agcgacaaga tcgccgcgct ggatgccgga gcggatgatt atctgagtaa
     8341 gccgtttggt attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc
     8401 caccgccgcg cccgatccgc tggtgaaatt ttccggtgtt accgtcgatt tagccgcccg
     8461 cgtgattcac cggggtgagg aagaggtgca tctcacacca attgagttcc gcctgctggc
     8521 ggtgctgctc aacaatgccg gaaaagtact cacccagcgc cagctcctta accaggtgtg
     8581 ggggccaaac gcggtcgaac acagtcacta tttgcgtatt tatatgggac atctgcgaca
     8641 aaaactggaa caggatccgg cccggcctcg ccacttcatt actgaaaccg gtattgggta
     8701 tcggtttatg ctttgaattg ttattcaata aattcgctta aatagtgatt aatttattat
     8761 taaacctgcg ccgaatatta ttatttggca ggtttaattt cttaacgcaa atataaaaca
     8821 cgaaattaca acatttaagt aacaaaacag catctaatat ggcgatattc ataattaatg
     8881 atttattatc tggggtatga tcgttttttg ttgatcttct tcacagatta tagccatttc
     8941 atggatagaa taactctacc ttcaactgac acagcaagcg gtaaaggtaa atggaaaata
     9001 ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt gcccatcgta
     9061 atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg gcaccccaac
     9121 gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtattcctc ggtgctcata
     9181 tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa cttgaggtat
     9241 gacgagtata gccagttacc gggctggtct gggttattgc atctgcaaaa agcaaactac
     9301 tgatttattt atcagcggtg gagctttgct ttttttccga cgtgatcgat ttctcctttg
     9361 agaaattgag gacctgctat tacctgaaat aaagagatga ataaaatgtc agaattaaaa
     9421 attgccgtta gccgttcttg cccggattgt ttttccactc atcgtgcatg cgtgaatatt
     9481 gacgaaagta attatattga cgttgccgcc attattttat cagtcaatga tgttgaacgt
     9541 ggaaaactcg ataaaataga cgctactggc tatggcattc ctgtttttat tgcaacggaa
     9601 aatgaagaac gtgtaccagc agaatatctg ccacgtattt ctggtgtctt cgagcattgc
     9661 gaatcacgga aagagtttta tggacgtcag ttagaaaccg ctgctagcca ttatgaaacg
     9721 caactgcgcc cgcccttctt ccgcgcactg gtcgattatg tcaatcaggg caatagcgct
     9781 tttgactgcc cgggtcatca gggaggtgag tttttccgtc gtcatccggc agggaatcag
     9841 ttcgtggagt acttcggaga aacgctgttc cgttccgact tatgcaacgc agacgtagca
     9901 atgggcgatc tgctgattca cgaaggcgcg ccatgcattg cacagcaaca tgcggcaaaa
     9961 gtgtttaatg ccgataaaac atacttcgtt ctaaatggca cttcatcttc taacaaagtg
    10021 gttttaaacg ccctgctaac accgggtgat ctggtgctgt ttgatcgcaa taaccacaaa
    10081 tctaaccacc acggagcgtt gctacaggct ggtgcaacac cggtttatct ggaaacggca
    10141 cgtaacccgt atggctttat cggtggcatt gatgcgcact gttttgaaga aagttacctg
    10201 cgtgagctga tcgcggaagt cgcaccgcag cgggcaaaag aggctcgtcc tttccgcctc
    10261 gctgtgattc agttaggcac ctacgacggt acgatttata acgcccgcca ggtggtggat
    10321 aaaattggtc atctgtgtga ctacattctg tttgactccg catgggttgg ctatgagcaa
    10381 tttatcccga tgatggctga ctgttcgccg ctgttgctgg atcttaatga gaacgatccg
    10441 ggtattctag ttacgcaatc cgtacataaa cagcaggctg gtttttctca aacttcacaa
    10501 atccataaaa aagacagcca catcaaaggg caacagcgtt atgtaccgca caaacgcatg
    10561 aacaacgcct ttatgatgca cgcctccacc agcccgttct atccgctgtt tgccgcactg
    10621 gatatcaacg ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg
    10681 gtaaatggca ttaatgcccg caaactgatc ctcgataact gtcagcatat tcgtccgttc
    10741 gtacctgaac tggtggatgg taaaccctgg cagtcgtatg aaacagcgca aattgcggtt
    10801 gatctgcgct tcttccagtt tgtaccaggg gaacactggc attcttttga aggctatgca
    10861 gagaatcaat actttgtcga tccttgcaaa ctgttgctga caaccccagg tattgatgca
    10921 cgtaacggcg aatatgaagc gttcggtgta cccgcgacga ttcttgctaa cttcctgcgc
    10981 gaaaatggcg tagtgccgga aaaatgcgat cttaactcca tcctcttcct gctgactccg
    11041 gcagaagata tggccaaact tcagcaactt gtgccctgct ggtacgcttc gaaaaactgc
    11101 ttgagtccga cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc
    11161 gctacgccgg ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc
    11221 acaacgtgaa acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca
    11281 tgaatccgca agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctgc
    11341 cggatgccga aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt
    11401 gtgttgttcc gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag
    11461 aagggatcaa cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac
    11521 atgatggtcg taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc
    11581 tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt
    11641 aaccatactg acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct
    11701 tgccgaagtt gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc
    11761 actggcatgg gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg
    11821 ttatgccgag tatgctttcg gtaaatccgg taactttatt gcgaactata cctacggcgt
    11881 ctcactgctg attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact
    11941 gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat
    12001 ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt
    12061 gtgggggggc attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc
    12121 gacgctttac gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc
    12181 ttccatcgct atgacgctgt gggcttttct tggtctggag tctgcgtgtg cgaatactga
    12241 tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc
    12301 ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct
    12361 ggcaaattca acggcaccat ttggcctggc cttcgcacag atgtttacac cagaagtggg
    12421 taaagtcatt atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt
    12481 caccattgcc caggtgttta aatcttcatc tgatgaaggc tacttcccta aaattttctc
    12541 ccgtgtaacc aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag
    12601 cgggttggcg ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa
    12661 cctggccgtg gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat
    12721 tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt
    12781 tggcgcgatg tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg
    12841 ttccatcgtg accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact
    12901 gaaaaataaa cacggttaaa aaagcaaagt aaaaaagcaa agtaaaaaag ggcgatcttg
    12961 cgaccgctct ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc
    13021 aaccgcttct ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat
    13081 cttatatgcg tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac
    13141 tttcagaccg ccaatagaag caccgttgcc cggagcagca gtcaggcgcg cggtgatcgg
    13201 gtcacctgcc agggtgctgg cgctcaccat ttccggagac agcttagaca gcgccgcttt
    13261 ttgtgcggaa gtcgcagctg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc
    13321 cagttcgttg tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag
    13381 cagacacata atgatgccgt ctttgtcggt ggaccacggc gtgccgtcga aacgcaggaa
    13441 ggaagcccct gcgctctctt cgccgccaaa gccgaagctg ccgtcgaaca gaccatcaac
    13501 aaaccatttg aaacctaccg gtacttctac cagcttacgg cccaagtcat tgaccacacg
    13561 gtcgatcatc gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg
    13621 acgatgctgg aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc
    13681 tgccggagtg acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa
    13741 cttatcacgc agtgccagca gacccgccat cgcacactcg gaggagcagt ccatacggat
    13801 cgcaccgtct ttatcgaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat
    13861 ggtcaggttg aggttgtaat actcgccaat gcgcttccag tattcgatac cggaaccgcc
    13921 caacggatca acgcccagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc
    13981 ggccagccct tccacgaacg gctgcaccag atcctgctct ttcacatagc cggatgccat
    14041 cgcttcgtcg agggagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc
    14101 cctgtcttcc accactttgg tgacgttagt atcagccggg ccaccatttg gcggattgta
    14161 cttgatacca ccatcttccg gcgggttatg ggacggtgta atcacgatac cgtctgccag
    14221 cgggccacct tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggagtgaa
    14281 gccattgttt tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga
    14341 aatgaacgca ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat
    14401 gccgtttttc gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt
    14461 aaagctgtgg cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc
    14521 cgcattccct gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat
    14581 caaatcactc tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt
    14641 tgtctgcaac gttttagata gttccgcaaa ccttctcaat caattccgcc gggaattgca
    14701 tcgactgcat gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca
    14761 cccaatacgg cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg
    14821 tttgttcatc tgccgcaaag tacacgcgtg tacgaccgtg caacgattcc gttgcttcgg
    14881 caaacgcctg ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg
    14941 ctcgcttttg ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt
    15001 ctttaatcgt tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt
    15061 tcgtcaccgg agcagcaggc tgtgatgcgg cggaaaattt caacatacgc cgtaaaatgt
    15121 cggatgcgct ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat
    15181 caacttcaat cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccaa
    15241 gagtatatga ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg
    15301 aataaacctt atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac
    15361 aacgcgaaaa aaagaacttt gccatgaaat taaatatccg cgcgcaaact gcacaaaacc
    15421 agcacaataa ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg
    15481 gcgtactggc tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc
    15541 acggtctttc accgagagag ccggtaatga attacccggc gatggcgcag gatcttgttg
    15601 ataccctgga tgcactgcag atcgacaaag caacatttat cggtcactcc atgggcggta
    15661 aagcggtaat ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg
    15721 atatcgcacc ggtcgactat cacgtacgcc gtcatgatga gattttcgca gctatcaacg
    15781 cggtcagtga atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc
    15841 ttaatgaaga aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct
    15901 ttaacgtgcc ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg
    15961 catgggatca ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt
    16021 accgtgatga tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg
    16081 gtcactgggt ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca
    16141 atgattaacg caactgatta aaaacccagc gtccgcgtcg ctttccgcga gtgggcgttg
    16201 gcgtgactca tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg
    16261 attgtttccc ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta
    16321 gatctgttcg cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat
    16381 gaacagctgc gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag
    16441 cgtgtcgaac tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggaatcg
    16501 cgtaatatga gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg
    16561 cgtagtcagg gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg
    16621 tttgctgatt ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt
    16681 ccacccccat ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt
    16741 tttcggcagc gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg
    16801 taaagacgtt gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta
    16861 cgacattctg ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga
    16921 tgacttcttc ccgactctcg aagagattga tttcaatggt aaactggttg cgctgtttgg
    16981 ttgtggtgac caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga
    17041 catcattgaa ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt
    17101 cgaagcatca aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga
    17161 ccgtcagccg gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga
    17221 gttgcatctc gacgaaattc ttaatgcctg atgtgatgcg gcgtagactc atgtctacgc
    17281 cgtattaata gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt
    17341 acctgtacaa tgtcccagtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg
    17401 tttttattaa caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata
    17461 atgatacgca ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta
    17521 gtaacaggac agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa
    17581 agtaacgctt cctcgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt
    17641 cagtgcggaa gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac
    17701 ggtatatcgc gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga
    17761 aggcggtaaa tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatttgcct
    17821 cgactgcggc aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat
    17881 tgccgcaaaa catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc
    17941 cgaaggcgat tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga
    18001 gaaaagccaa cctgcgggtt ggctttttta tacaagggaa ataagaaaag cgagagttac
    18061 agctctcact tatttgttat tactgcgtat ttcctgccag accttatcac aatcggcttt
    18121 caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg
    18181 tacactttcc tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac
    18241 gctctggcag gcttcaattt tttctggcga accttgcgcg gtgttgatac aggcgctgta
    18301 tgcctctttc agacggctgt cttcctgtgg tgcggttgat tgcgcacaag ctaccagccc
    18361 cgatgccatc atggcgatga gaatcagttt tttcattgaa tgcggcaggc acaaaacctg
    18421 ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc ataaatttca cgtcacgctc
    18481 atcctggaag atgttaccgt aaccgccgcc ccagcttggg atgtcggagt ggttgtcgta
    18541 ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa tggtgtagac
    18601 cgcatccagg ctgtaggcag actcttcaat agtccggttt ttgtcgtagt acgcatccgg
    18661 attgctctgc caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc
    18721 gaagcctgga agattccagt ttttcaggtc atacatcgca ctgaagaaga ccgctttttc
    18781 gccgttagcg ttgaagtcgg aacggttatc ccaccagata tccaggcggc cgttggagga
    18841 agcgtaggtt ggggtcatac gttgcaggaa gtatccctgc tgactgtcag ccttaatcca
    18901 ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg taaccaaagg tcaacgcttg
    18961 cagccaggcg gtgccgtcat aaagatcgtt gacgctgcga tcgtcaactt tatcgcgggt
    19021 accatagaac tggtagctgg tggttaacgg gctaccggcg atatcaaatt tgtagctggc
    19081 tttggcaaaa tattgatcga tatacccttc cgcctgacca aatgccgctt ccagtacgaa
    19141 gttatttttg aagtcgtatt tcgcaccgat ggagtgcaga taatcaactt tggtggtttt
    19201 atcgttctga taaaactcat ccatttccag atgccacggc gctttgtatt cgttggtcca
    19261 catgtaggag aaactcaagg caccagcatc gccgtaatca aaattcgccc cggcttccgc
    19321 cccctgataa gtacccggca taaagctcca gtgcggcgct aacagcgttt gaccggttgg
    19381 ctgaatgtaa cctgccctcg cccaaaccgg accgtattta aatttggccg cagctttata
    19441 caggcttata ccgcttttgt cgccggacca gtcttcgtca taggctttat tactttttga
    19501 aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc gccatttcaa tcgccgtaaa
    19561 cgcagcaata tcaaggccga acatatcagc agcataaccg gactgaaaat cgaggttggc
    19621 attccaggtg gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa catctttacg
    19681 gtcgcgttca cgctgccagt aatagatacc gccggttaag gttgaatcgt cgatgaatcc
    19741 ttcggcgcga gcctccggca ttgccataaa gcccgacatt gctgtaacac cggcaatagc
    19801 cagcgccagc gtactacgtt tgccactaaa cgtacgcatg ggttaatcct ctttgacgta
    19861 taaattgctg caccaaaggt gaacagcgaa aaaaaataaa aattaaaaaa gtagcgttga
    19921 gaaaagtctc gctgactaca ggaacagact atttttcgcg acgcgaatta tcaatctctt
    19981 tctgtaacca gaatgtaaga acatgagcaa ccgcacatat tttgatgatt tttgttacaa
    20041 ggcttaagtt tgcggttgaa aggcttgcag aagaataaaa acgtgaaatt ccagcaagat
    20101 gaaagcggtt acattttatt taattatgtt ttgtgatttt aaatttattt tatggataaa
    20161 ttgcgaagcg gaattaattc gcgaagaaaa gcagaaaaaa cgccgcagag cgcggcgttt
    20221 gggattgttt gctatttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc
    20281 acgcaaattc aatatattgc agagatcatg taggcctgat aagcgaagcg catcaggcaa
    20341 tttagcgttt gaaatttatt gtctatttat tgccggatgc gacgtaaatg cctcacccgg
    20401 cttggtagaa cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg
    20461 cggttaaata ccggtttttc ggccgtggaa tggcggctgt cgaggcagaa gtaaccttca
    20521 cgctcaaact ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc
    20581 tgtttgatca ccagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg
    20641 ttaggcacgc tgaacagacg atcatacaaa cggatttcaa ccggcagcgc atgtgccgcg
    20701 ctcacccagt gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg
    20761 tcggcgtcat aagtacagaa gatggtggtg atattacctt cggcatcttt ctccacgcgt
    20821 tcagccttaa tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg
    20881 tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga tccaaatttc accgctaaac
    20941 ggcacctgac ggctccccat ttccggtttg ttcggatggt tcggcatggt gaccatttcg
    21001 ccttcgccct gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc
    21061 ggcgcatttt cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg
    21121 ttgtcctgct tggtcacgcc gatgcgtttg cagaactcac gaatagaagc cgcagtgtaa
    21181 ccacgacgac gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc
    21241 ttgtcggtca ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg
    21301 cgcgagaatt cgtactggcg cggatgaaca ggaatggtga tgttatccag tacccagtcg
    21361 tacagacgac ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagc
    21421 gcatcgctga tgcaatgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc
    21481 tggtggtgtt cagcaaattt aatgcggtac agcaccggat cgcgcattac gataaatggc
    21541 gacgccatgt cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc
    21601 attttttcga acagcgccaa gttctcttca acgctgcggt cgcggtacgg gctgttttta
    21661 cccggctgcg tcagggtacc gcggtattcg cggatctgtt ccggtgtcag ttcatcaacg
    21721 tacgccaggc ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg
    21781 gaggagtaac ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc
    21841 gaatcgacat actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac
    21901 tggcctttat agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata
    21961 tgcagatagc cattcggctc cggcgggaaa cgggtgtgta ctgtgatgtg cttaccactg
    22021 gccagatctt catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc
    22081 atcgtggatt cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca
    22141 ggctgacaac tgttagtttt ttaaaattga taatctaaca aggattatgg ggatgattga
    22201 tgaaaaaaaa agcggcagag aagatctcca ccgcttgtca ttgttggatg cgacgctgcc
    22261 gtgtcgcatc aggcataaag cagattactt tttgatttca tacagcggtg tttgacccgc
    22321 cacaacatgg ccctgagctt taatgagcaa gccactgaaa tcgtcgatat tgctgcaaac
    22381 caccgggcta atcatcgagc gggcgttagc gttcaggtaa tccagatcca tttccagaat
    22441 cggttgccct gcgcttacct gcgcaccctc ttccaccaga cgtttaaagc ctttaccttc
    22501 cagcgctacg gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag
    22561 gcagaacgcg tggttggtgt tgaagatttt cacgattgtc cctgcggctg gtgatacgac
    22621 gattttatct gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc
    22681 gtcaggaacc tgatccagtg ccacgacatc accggtaatc ggcgatacca gctccgcgat
    22741 agataccgcg tttggtacag cctgcggttt tgctacaggc gcggcagttg ccggagttgc
    22801 ttcagctgac gcagcggcta ccggaccacg ggcaacgact ttcttcatcg catcgccgat
    22861 ggattctgct ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt tcactacacc
    22921 agaagcaccc agacgtttac acatcgtatc gttgacgcgg gcagagtcag ccacggtcag
    22981 gcgcagacgg gtaatacagg catcaatcgc tttcaggttg tcagtaccgc caaccgcagc
    23041 aatatagttg gttgccagtt gagtcagacc ttcttcagta ttgctgttgg cttcttcagt
    23101 aacgatctcg tcttctttat cttcacgacc cggcgttttc aggttgaaca tgcggataac
    23161 caaactgaac accacgaagt agatagcgaa gaagataacg cccatcacca gcagcatcca
    23221 gacgttctgg ctggcggcag gcaggttata catcaacgcg tagtcgatag cacccgcaga
    23281 gaaagagaag ccggcatgga tacccagcag cgttgccaca aacaggctga taccggtcag
    23341 cagtgcgtgc aggaggtaca gcagcggcgc aaggaacatg aacaggaatt ccagcggctc
    23401 agtcacaccg gtcaggaacg cagtaacagc aacagaaagc agcatcccgc caaccatcgg
    23461 acgacgctct ttcggtgctg cgaagtacat cgccagcgcc gcacccggca gaccgaacat
    23521 catgatcggg aagaagccgg acatgaacat ccccgcggtg ccgtcaccag cgtagaaacg
    23581 gttgatgtcg ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa tctggaacca
    23641 ggcgatggtg ttcagcacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc
    23701 aaagataccg gaacccagcg cgcccgcaga aacgatccac tcgccgcctg catggatagc
    23761 gtgctgtacc ggcggccaga cgtaaccaaa aatggccgcc agcaccagac agaagaagcc
    23821 ggtggcgatc ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat
    23881 atcggaccaa cggttatagg ctgcgccacc aaccagaccg gtaatgatac ccgccagtac
    23941 acccatgtta atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac
    24001 cgcacccgcc agcgccgccg cacctgcgct gtctttcgac cagctggatg ccacaccgat
    24061 ggcgaagatt aatgcgaggt tatcaaaaat tgcaccgccc gcctgggcaa taaacgcaac
    24121 gttaagtaaa tctggctgac cgaatcgcag caacagcgcc gccaccggca gcaccgcgat
    24181 agggagctgt aacgccctac cgagtcgctg gaaaaaacct aaaatattca tcttattccc
    24241 cctacgagaa ccctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattttac
    24301 tgccgatgtt ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat
    24361 tcgcatcgca aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc
    24421 actccctttt actggctaaa ccagaaaact tattttatca ttcaaaaaat caagtcggat
    24481 tgacgcctgt ctgcgcaaat ccaggttacg cttaaagatg tctaatccgc caacggctta
    24541 cattttactt attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg
    24601 gcaaatgggc tgctcgccat atcgtcaatc gtatcaatgc gttcaaacca actgccgatc
    24661 gtccgtttgt actgggcctg ccgactggcg gcacgccgat gaccacctat aaagcgttag
    24721 tcgaaatgca taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg
    24781 aatatgtcgg tctgccgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt
    24841 tcttcgatca cgttgatatt ccagcagaaa acatcaacct tctcaacggc aatgccccgg
    24901 atatcgatgc cgagtgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc
    24961 tgtttatggg cggtgtaggt aacgacggtc atattgcatt taacgaaccg gcgtcttctc
    25021 tggcttctcg tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt
    25081 tctttgataa cgatgtcaat caggtgccaa aatatgccct gactgtcggt gttggtacac
    25141 tgctggatgc cgaagaagtg atgattatgg tgctgggtag ccagaaagca ctggcgctgc
    25201 aggccgccgt tgaaggttgc gtgaaccata tgtggaccat cagctgtctg caactgcatc
    25261 cgaaagcgat catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa
    25321 gatatttcaa tgaattagaa gcagaaaata tcaaaggtct gtaattgtta tccctgccct
    25381 ggttccttgc tcagggcaat attttttaaa atcgggggtc agaatgtatg cattaaccca
    25441 gggccggatc tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg
    25501 cctgattaaa agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttcact
    25561 gaacggggcc attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt
    25621 acagtttaac gacaccgctg aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc
    25681 caatgagaaa tcaggctgta ctaactatct gccgacgctt atcaccacca gcgatgagct
    25741 gatgaaacag ggcgtgcgcg ttatgcgtga gtacctggca aaacatccga atcaggcgtt
    25801 aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa
    25861 ttttgtgcgt aagcctgatg ccgcgctggt cgatttcctg tgtgaaaacg ccgacgtcat
    25921 taccaaagtg accctggcac cggaaatggt tcctgcggaa gtcatcagca aactggcaaa
    25981 tgccgggatt gtggtttctg ccggtcactc caacgcgacg ttgaaagaag caaaagccgg
    26041 tttccgcgcg gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg
    26101 tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat
    26161 cgctgatggc ctgcatgttg attacgccaa cattcgtaac gctaaacgcc ttaaaggcga
    26221 caaactgtgt ctggttaccg acgccaccgc gccagcaggt gccaacattg aacagttcat
    26281 ttttgcgggt aaaacaatat actaccgtaa cggactttgt gtggatgaga acggtacgtt
    26341 aagcggttca tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgcggtat
    26401 cgcactggat gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga ttgacgttga
    26461 aaaacgtctc ggcacgctcg ccgcaggtaa agtagccaac ctgaccgcat tcacacctga
    26521 ttttaaaatc accaagacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt
    26581 atgacaccag gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc
    26641 gcggcggttt atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag
    26701 caaagccagc ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg
    26761 ctgatcaaag aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc
    26821 accgaaaccc gcaatttcca cgcaatcggc gtacggcttg gtcgtcatga cgccaccatc
    26881 actctgtttg atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt
    26941 acccagcaga cgctggaaca tgccctgctg aatgccattg ctcagtttat tgatagctac
    27001 cagcgcaagc tgcgcgagct aatcgccatt tcggtgatcc tgccagggct tgttgacccg
    27061 gacagcggca aaattcatta catgccgcat attcaggtag aaaactgggg gctggtagaa
    27121 gcactggaag aacgttttaa agtgacctgt ttcgttggtc acgatatccg tagtctggcg
    27181 ctggcggagc actacttcgg tgcaagtcag gattgcgaag actccattct ggtgcgtgtc
    27241 catcgcggaa ccggggccgg gattatctct aacgggcgca tttttattgg ccgcaacggt
    27301 aacgtcggtg aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc
    27361 aactttggct gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg ggtgttgaat
    27421 ctgttaaagc agggctacca gagccgtgtg ccactggacg actgcaccat caaaactatc
    27481 tgcaaagccg cgaacaaagg cgatagcctg gcctcggaag tgattgagta tgtcggtcgt
    27541 catctgggta aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt
    27601 gctggtgaaa tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat
    27661 acccaagcgc tgaaggcgtt tcgtactaat ctgccggtgg tacgttctga gctggatcac
    27721 cgctcggcaa tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc
    27781 cagcatttgc tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg
    27841 ggtagtccat gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata
    27901 acgtcgccgt accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc
    27961 tggtgttgct gaccaactat ccttcgcaga ctgggcaaga tctggcgaac cgctttgcca
    28021 ctgccggtgt cgatgtgccg gatagcgtgt tttatacctc tgcgatggcg accgccgatt
    28081 tcctgcgtcg ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg
    28141 aactgtacaa agccggtttc actattaccg atgtgaaccc tgatttcgtg attgttggcg
    28201 aaacacgttc ctacaactgg gacatgatgc ataaagcagc ctatttcgtc gctaacggtg
    28261 cacgctttat cgccaccaac ccggacaccc acgggcgcgg tttttatccc gcttgtggcg
    28321 cgttgtgtgc agggattgag aaaatctccg ggcgcaaacc gttctatgtt ggtaagccca
    28381 gcccgtggat catccgcgcg gcattaaaca aaatgcaggc gcattcggaa gaaacagtga
    28441 ttgtcggcga taacctgcgt accgatattc tggcgggctt ccaggccggt ctggagacga
    28501 ttctggtgct ttctggtgtt tcatcgctcg acgatatcga cagtatgccg ttccgcccaa
    28561 gctggattta cccgtcagtc gctgaaatcg acgttatctg aataccaccc acgtctgcgg
    28621 acgtggttgg ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt atcaggccta
    28681 cgcagctcct gcaatatatt gaattttcat gcttttgtag gccggataag gcgttcacgc
    28741 cgcatccggc atgaacaaat cgcactttat cagcaatttg gcccccgtaa aatataacca
    28801 ttcaataaaa cccgctaaaa attatcgatt catcaataaa tacatcctca caccagaccc
    28861 tttttcacca atcgacaatc accattacgt tttttatttt tgcactgcca aatagcttgc
    28921 gcacagtgct gctttgcggc atttttttaa acaagcaaac acaacaagca acaaatacca
    28981 ggttaacgga gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa acagacgcag
    29041 ttgagctgcg taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact
    29101 ggtccggtat ttatgccagc gataacgcca ttctcgctca cgaacgtctg tcaattgttg
    29161 acgttaacgc gggggcgcaa cctctctaca accaacaaaa aaaccacgta ctggcggtaa
    29221 acggtgaaat ctacaaccac caggcattgc gcgctgaata tggcgatcgt tatcagttcc
    29281 agacagggtc tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc
    29341 tcgacgactt gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc
    29401 tgattggtcg cgaccatctg gggatcatcc cactgtatat ggggtatgac gaacacggtc
    29461 agctgtatgt ggcctcagaa atgaaatcgc tggtgccagt ttgccgcacg attaaagagt
    29521 tcccggcggg gagctatttg tggagtcagg acggcgaaat ccgttcttat tatcatcgcg
    29581 actggttcga ctttgatgca gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg
    29641 cactggaaga ttccgttaaa agccatctga tgtctgatgt gccttacggt gtgctacttt
    29701 ctggtggtct ggattcctca attatttccg ctatcaccaa gaaatacgca gcccgtcgcg
    29761 tggaagatca ggaacgctct gaagcctggt ggccgcagtt acactccttt gctgtaggtc
    29821 tgccgggttc accggatctt aaagcagccc aggaagtggc aaaccatctg ggcacggtgc
    29881 atcacgaaat tcacttcact gtacaggaag gtctggatgc catccgcgac gtgatttacc
    29941 acatcgaaac ttatgatgtg accactattc gcgcttcaac accgatgtat ttaatgtcgc
    30001 gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt
    30061 ttggcggtta tctttacttc cataaagcgc ccaacgctaa agaactgcat gaagagacgg
    30121 tgcgtaaact gctggccttg catatgtatg actgcgcgcg cgccaacaaa gcgatgtcag
    30181 cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa attccttgat gtggcgatgc
    30241 gcattaaccc gcaggataaa atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg
    30301 aatgttttga gtcatacctg cccgcaagcg tggcctggcg gcagaaagag cagttctccg
    30361 atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag caggtttctg
    30421 atcagcaact ggaaactgcc cgcttccgct tcccgtacaa cacgccgacc tcaaaagaag
    30481 cgtatctgta ccgcgagatc tttgaagaac tgttcccact tccgagtgcc gctgagtgcg
    30541 tgccgggcgg tccttccgtc gcgtgttctt cagctaaagc gatcgaatgg gatgaagcgt
    30601 tcaaaaaaat ggacgatccg tctggtcgtg cggttggcgt tcaccagtcg gcgtataagt
    30661 aagaaatgcg gtactgcccc ggagaaatcc ggggctgttt tagttattgc gctgcttgtt
    30721 gattctgata tgcagtaagg aaatgttccg ggtttgccac atcactgttt gacaactcat
    30781 cactgtaggc cagtatttca tagattaatt tcccaccact attgttgaat tccgaatttc
    30841 atcctgtacg acaaaagaat ggctgtaggg tggggaaaaa cacaaagtaa acaataattg
    30901 acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga gcaattttcc
    30961 ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc aacgccgata
    31021 aggtatcgcg aaaaaaagat ggctacgtag ctcagttggt tagagcacat cactcataat
    31081 gatggggtca caggttcgaa tcccgtcgta gccaccatct tttttgcggg agtggcgaaa
    31141 ttggtagacg caccagattt aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc
    31201 tcccgcacca ttcaccagaa agcgttgtac ggatggggta tcgccaagcg gtaaggcacc
    31261 ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc ttcttcgagt
    31321 aagcggttca ccgcccggtt attggggtat cgccaagcgg taaggcaccg gtttttgata
    31381 ccggcattcc ctggttcgaa tccaggtact
//
LOCUS       DAJVRL010000041        31188 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVRL010000041 DAJVRL010000000
VERSION     DAJVRL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.041"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            389..2047
                     /locus_tag="OQN02_001881"
     CDS             389..2047
                     /locus_tag="OQN02_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1642689.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="OQN02_001882"
     CDS             complement(2044..3000)
                     /locus_tag="OQN02_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS1642690.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="OQN02_001883"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="OQN02_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1642691.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="OQN02_001884"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="OQN02_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1642692.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OQN02_001885"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OQN02_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1642693.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="OQN02_001886"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="OQN02_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1642694.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="OQN02_001887"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="OQN02_001887"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1642695.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="OQN02_001888"
     tRNA            complement(9859..9946)
                     /locus_tag="OQN02_001888"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OQN02_001889"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OQN02_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1642696.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OQN02_001890"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OQN02_001890"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1642697.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OQN02_001891"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OQN02_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1642698.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OQN02_001892"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OQN02_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1642699.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OQN02_001893"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OQN02_001893"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1642700.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="OQN02_001894"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="OQN02_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1642701.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="OQN02_001895"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="OQN02_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1642702.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGTAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="OQN02_001896"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="OQN02_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1642703.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="OQN02_001897"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="OQN02_001897"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1642704.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="OQN02_001898"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="OQN02_001898"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1642705.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27413
                     /gene="dmsA"
                     /locus_tag="OQN02_001899"
                     /pseudo
     CDS             24969..27413
                     /gene="dmsA"
                     /locus_tag="OQN02_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27424..28041
                     /gene="dmsB"
                     /locus_tag="OQN02_001900"
     CDS             27424..28041
                     /gene="dmsB"
                     /locus_tag="OQN02_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1642706.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28043..28834
                     /locus_tag="OQN02_001901"
     CDS             28043..28834
                     /locus_tag="OQN02_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1642707.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28870..29496)
                     /locus_tag="OQN02_001902"
     CDS             complement(28870..29496)
                     /locus_tag="OQN02_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1642708.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29811..30959
                     /locus_tag="OQN02_001903"
     CDS             29811..30959
                     /locus_tag="OQN02_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1642709.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7688 a         7643 c         8378 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 gcgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ttgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct agtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acggggactg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatgg cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgtg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagccgcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgatcgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgatcg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctaccgat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc
    25261 tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcaccgtgtc tacaatccgg
    25321 accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca
    25381 ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt
    25441 acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc
    25501 gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc ggcggttatc
    25561 ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac catacctacg
    25621 gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg gtagtgctgt
    25681 ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac
    25741 aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat accgacaccg
    25801 gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc gcactggtta
    25861 acggtctggc gtacgtcatg atcactaaaa acctggtgga tcaggcattc ctcgataaat
    25921 attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat ggtcattata
    25981 aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcgc
    26041 aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca
    26101 aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa
    26161 cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat ggagggaaca
    26221 gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc
    26281 cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa
    26341 tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg atcaaaatga
    26401 tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg
    26461 aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct
    26521 catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact
    26581 ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta
    26641 aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg
    26701 gcgttgagca acagtttact gaaggccgta cctaggaaga gtggatgcgg catctgtatg
    26761 cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga
    26821 tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc
    26881 cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg caggcgctgg
    26941 ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct
    27001 acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta
    27061 caggtttcca ctataaatct cgcgttcact caacttacag caacgttgat gtgctgaaag
    27121 cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc ggtatccaca
    27181 acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag gcgaaagtga
    27241 cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat gacccggatg
    27301 caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt ccgtctcctc
    27361 tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa
    27421 ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc
    27481 tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt ccgccgcatt
    27541 tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt
    27601 gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc
    27661 ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga agatgtatgc
    27721 attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata taacgaaacg
    27781 aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg
    27841 atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt
    27901 aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg
    27961 aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg
    28021 gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt
    28081 cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa
    28141 aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt
    28201 actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga tgcgcgcctt
    28261 taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat
    28321 cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc
    28381 attgcgtacg ctatggctgg tggtgacgat ggttcttggc gtcgtctttg tctggatgat
    28441 ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat
    28501 gggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt
    28561 gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc
    28621 agcgctggtg ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt tggccgttgc
    28681 cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc
    28741 cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt
    28801 gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc
    28861 ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc
    28921 aggaatatgg ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg
    28981 cagctcgcaa gccacgccga accatgtcat caattgcgca ccagcttgcg acatgcgatc
    29041 ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat gcatcagtca cgacaaagac
    29101 atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt cggttaccac
    29161 accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt
    29221 atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga attgtgcttt
    29281 cagttctaga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg
    29341 taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg
    29401 ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc
    29461 atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt
    29521 ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg
    29581 agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc
    29641 aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat
    29701 ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt
    29761 ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt
    29821 atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg
    29881 tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag
    29941 gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg
    30001 tcattaaacg cattggcttt aaccgcagct attatctgtc ctccttcatt tttgctgctg
    30061 gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg
    30121 cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg tgcagtggga
    30181 cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacgt
    30241 ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg
    30301 ttacaggttt gacattggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc
    30361 aggctgaaaa ccatgattcg acgtcaatta cggcaatgct aaaactccgt caggcgcggc
    30421 ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat ggcctgatgc
    30481 ctctgtacct caatcacaaa ggggtgagca atgccagtat tggtttctgg atggcggtac
    30541 tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc
    30601 gactgctggt tttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta
    30661 gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc
    30721 cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc
    30781 aagccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt agcgctatgc
    30841 taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct
    30901 atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa
    30961 tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct
    31021 gacttgcttt tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag gttataattt
    31081 tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt ttacagatgc
    31141 ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcagga
//
LOCUS       DAJVRL010000042        31047 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVRL010000042 DAJVRL010000000
VERSION     DAJVRL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.042"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="OQN02_001904"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="OQN02_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="OQN02_001905"
     CDS             1128..1235
                     /locus_tag="OQN02_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642710.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="OQN02_001906"
     CDS             complement(1288..2046)
                     /locus_tag="OQN02_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1642711.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="OQN02_001907"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="OQN02_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1642712.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="OQN02_001908"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="OQN02_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1642713.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="OQN02_001909"
     CDS             3759..4619
                     /locus_tag="OQN02_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1642714.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="OQN02_001910"
     CDS             complement(4660..5196)
                     /locus_tag="OQN02_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1642715.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="OQN02_001911"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="OQN02_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1642716.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="OQN02_001912"
     CDS             6174..6764
                     /locus_tag="OQN02_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1642717.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="OQN02_001913"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="OQN02_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8291..9107)
                     /locus_tag="OQN02_001914"
                     /pseudo
     CDS             complement(8291..9107)
                     /locus_tag="OQN02_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="OQN02_001915"
     CDS             complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="OQN02_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS1642718.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9830..12091
                     /gene="katE"
                     /locus_tag="OQN02_001916"
     CDS             9830..12091
                     /gene="katE"
                     /locus_tag="OQN02_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS1642719.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="OQN02_001917"
     CDS             complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="OQN02_001917"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS1642720.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12909..14261)
                     /gene="celF"
                     /locus_tag="OQN02_001918"
                     /gene_synonym="chbF"
     CDS             complement(12909..14261)
                     /gene="celF"
                     /locus_tag="OQN02_001918"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1642721.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="OQN02_001919"
                     /pseudo
     CDS             complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="OQN02_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="OQN02_001920"
     CDS             complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="OQN02_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS1642722.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="OQN02_001921"
     CDS             complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="OQN02_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCS1642723.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="OQN02_001922"
     CDS             complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="OQN02_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1642724.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="OQN02_001923"
     CDS             complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="OQN02_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1642725.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18218..19045
                     /gene="nadE"
                     /locus_tag="OQN02_001924"
     CDS             18218..19045
                     /gene="nadE"
                     /locus_tag="OQN02_001924"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1642726.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19275..20162
                     /gene="cho"
                     /locus_tag="OQN02_001925"
     CDS             19275..20162
                     /gene="cho"
                     /locus_tag="OQN02_001925"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1642727.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20122..20697)
                     /gene="ves"
                     /locus_tag="OQN02_001926"
     CDS             complement(20122..20697)
                     /gene="ves"
                     /locus_tag="OQN02_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1642728.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20900..21385)
                     /gene="spy"
                     /locus_tag="OQN02_001927"
     CDS             complement(20900..21385)
                     /gene="spy"
                     /locus_tag="OQN02_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1642729.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21715..22683)
                     /gene="astE"
                     /locus_tag="OQN02_001928"
     CDS             complement(21715..22683)
                     /gene="astE"
                     /locus_tag="OQN02_001928"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1642730.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22676..24009)
                     /gene="astB"
                     /locus_tag="OQN02_001929"
                     /pseudo
     CDS             complement(22676..24009)
                     /gene="astB"
                     /locus_tag="OQN02_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24006..25484)
                     /gene="astD"
                     /locus_tag="OQN02_001930"
     CDS             complement(24006..25484)
                     /gene="astD"
                     /locus_tag="OQN02_001930"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCS1642731.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25481..26515)
                     /gene="astA"
                     /locus_tag="OQN02_001931"
     CDS             complement(25481..26515)
                     /gene="astA"
                     /locus_tag="OQN02_001931"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1642732.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPRYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26512..27732)
                     /gene="astC"
                     /locus_tag="OQN02_001932"
                     /pseudo
     CDS             complement(26512..27732)
                     /gene="astC"
                     /locus_tag="OQN02_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28178..28984
                     /gene="xthA"
                     /locus_tag="OQN02_001933"
     CDS             28178..28984
                     /gene="xthA"
                     /locus_tag="OQN02_001933"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1642733.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29151..29859
                     /locus_tag="OQN02_001934"
                     /pseudo
     CDS             29151..29859
                     /locus_tag="OQN02_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29864..30541
                     /gene="ydjY"
                     /locus_tag="OQN02_001935"
     CDS             29864..30541
                     /gene="ydjY"
                     /locus_tag="OQN02_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS1642734.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30556..>31047
                     /locus_tag="OQN02_001936"
     CDS             30556..>31047
                     /locus_tag="OQN02_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1642735.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPIS"
BASE COUNT         8018 a         7969 c         7294 g         7766 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttccagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattaacca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaatcatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa
     8401 tgtccaactt atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa
     8461 cctcataccc gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca
     8521 caccacttcc cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg
     8581 atattatcga aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc
     8641 ccggcaggat gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac
     8701 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
     8761 atcccaacaa aattctcatt aatatctctt atccgaaact cactgccata aagattatta
     8821 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggcgct
     8881 cctagctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
     8941 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
     9001 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
     9061 ccataattac tctgtattaa gtttgtagaa aacatctccc gcctcatatt gttaacaaaa
     9121 ttattatctt atttaaatct aagtcattta caatataagt ttaagagcga cgccacagga
     9181 tgaactatca aaaataactc atcatgatta gcaaaactta accattttaa aataaataaa
     9241 taattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
     9301 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcctg taactcccgg
     9361 caaaaccggg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
     9421 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
     9481 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
     9541 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
     9601 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
     9661 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     9721 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     9781 ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca
     9841 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     9901 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     9961 gccaggtgca caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa
    10021 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
    10081 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
    10141 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
    10201 acgtattgtt catgcacgcg gctcagcagc tcacggttat ttccagccat ataaaagctt
    10261 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
    10321 taggttctct accgttcagg gtggagctgg ctctgccgat accgtgcgtg atatccgtgg
    10381 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
    10441 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
    10501 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
    10561 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
    10621 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
    10681 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
    10741 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
    10801 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
    10861 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
    10921 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
    10981 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
    11041 cgatcccctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
    11101 tgggccgaat ttccatgaga ttccgattaa ccgcccgacc tgcccttacc ataatttcca
    11161 gcgtgacggc atgcatcgta tggggatcga cactaacccg gcgaattatg aaccgaactc
    11221 gatcaacgat aactggccgc gcgaaacacc accggggccg aaacgcggcg gttttgaatc
    11281 ataccaagag cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata
    11341 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
    11401 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
    11461 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
    11521 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
    11581 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc
    11641 gattttactt aatgatgaag tgagatcggc agaccttctg gccattctca aggccatgaa
    11701 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
    11761 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
    11821 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
    11881 tctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
    11941 taaagcaaca atcaaggtag ctgaccaggg tgaagaaggg attgtggaag ctgacagcac
    12001 cgacggtagt tttatggatg aactgttaac gctgatggca gaacaccgcg tgtggtcacg
    12061 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
    12121 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
    12181 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
    12241 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
    12301 ggatcacccc gatggcttgc gtcatccagc acttgcagga acagtgcctc gctaatctct
    12361 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattaaccgg taaatcacca
    12421 gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg
    12481 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
    12541 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
    12601 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
    12661 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
    12721 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
    12781 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
    12841 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
    12901 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
    12961 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
    13021 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
    13081 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    13141 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgtgcccca gcttacaggt catttctacc
    13201 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    13261 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    13321 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
    13381 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    13441 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    13501 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    13561 ccagatactt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgcgacttg
    13621 ccatttacca gcacatcctt aatgaacacc atatggttga ggccaaacaa atcgatagat
    13681 aaatcatcgc tgtctttcag catcagaaca tcgtgaataa acatcttcat gccgatcgga
    13741 atattacaca cgccgataaa acgtttaaat ccggtatgac gataaacggc ttcagtaacc
    13801 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
    13861 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacaaacc gcccgcgccg
    13921 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcacgcgcc
    13981 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    14041 caatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    14101 agatcgaaaa taatatccag tttagctttg ccaccctcga catccaccag ccataattcg
    14161 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    14221 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    14281 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
    14341 tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc ttacgatatc
    14401 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
    14461 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgtttttttg
    14521 caaaattaat acggatctca ttaataatct gcattggtgt tttgccataa tatcgttgag
    14581 tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg
    14641 attcactaaa ctgctcttta tcatgcatct tttctaccgt acttttcagc cactacggta
    14701 catcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa
    14761 ccatctcaac aaactcttcc agccctgttt cgcggaaatt taatgaagaa atcactgttt
    14821 ccacataggt gagaaaagcg ttattggtcc ggtatacctg cgaagcaaca aagcaataag
    14881 gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatgc
    14941 gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata aaaacaaaat
    15001 cgccccgttc cagtaacacg cgcttaccgt taatctcctg gaaataacgc ccggttaata
    15061 ctagagtaaa ttcataatag tcgtgctgat gcagtccgct gatactctca gttttgttat
    15121 agataaacac atggaagttt ttgccattaa acaactgctg ttctcgggca gtggcaattt
    15181 ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc
    15241 tcaattaatt cagtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg
    15301 tggaccagca ccagactcac tttcatctta ccttcgcccg catcgccttc aatcagtttc
    15361 gtctgtacca gatgcgcttc attcaatgcc atccgtgact gatccatcat ggctttcgct
    15421 gcggcaaaat cgccctgctt cgcctgtttc aatgcggcat acgccaggct acgcgcttgt
    15481 ccagagttga tgataagccc catcaccact tcttccagct cttcagcttc tgtttgcgta
    15541 tcaggaatat tatcgagatc catcatacat cgttcctctc tcttcttacc ggcaagatta
    15601 cccgtaccgg catcgattaa aatttcaggg gttagcgata tcttcttcgc tctcttcttt
    15661 atcaatcgca ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaacgttgc
    15721 gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
    15781 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaatgatgcc
    15841 catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
    15901 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
    15961 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
    16021 aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
    16081 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
    16141 accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa tgccgttgtc
    16201 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
    16261 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
    16321 aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac
    16381 ggaaagaata ataaagccgg gaattaatgc cgagaaagaa cgcgatactg aagcaggtac
    16441 gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
    16501 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
    16561 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
    16621 cccagccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
    16681 tgccattaaa gacattattc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
    16741 accattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga
    16801 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
    16861 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
    16921 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
    16981 atacccttaa aaaccgcaat ttaaatatta cggtattgat ttatgaaata actctttgac
    17041 gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt
    17101 aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt
    17161 gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac
    17221 gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac
    17281 cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag agatagacat
    17341 gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat
    17401 tatctgtcag ccagacactc cgcaagcctt gacctgcttc catgctctgg gtaacttgcg
    17461 aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt
    17521 atttctttga ccttataaat tgtttctcct tgacctgtgg ttatgaccct cttcacattt
    17581 cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc
    17641 cggtttccgt tgttactcaa gctttcagac gaattgatta cttcgcagcc tgtggatcag
    17701 tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg
    17761 cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac
    17821 cgcgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg
    17881 cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac
    17941 gatcataagc tgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg
    18001 ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata
    18061 cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat
    18121 gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct
    18181 ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat
    18241 aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga
    18301 ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag
    18361 cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg
    18421 ccaggaaact cgcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca
    18481 ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac
    18541 cgttaatatc aagggcgcgg tattggctag cgagcaggca ttgcgggaag caggcattga
    18601 actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag
    18661 cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac
    18721 cggattcttc actaaatatg gtgacggcgg tacggatatt aatccgctgt atcgtctcaa
    18781 caaacgccag ggtaaacagt tactgacggc attaggttgc ccggaacacc tttataagaa
    18841 agccccaact gccgatcttg aagatgatcg cccttctctg ccagatgaag tggcactcgg
    18901 cgtgacctat gacaatatcg acgactatct ggaagggaaa aacctacctg aacaggtcgc
    18961 cagaacaata gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaacgt
    19021 tttcgatgat ttctggaaaa agtaataatt tgcacctatt ggattgtgcg aaaaagagta
    19081 atttgttcac gccggatgcg gcgtgaacgc cttattcgac ctacaaaact atgcaaattc
    19141 aatacattgc aggagtcgaa taggcctgac aggcgtagca cgtcagacgg tgtaaccttt
    19201 gtcatcaacc cgcctctttt ttaatcgcct cccgcctgtt acactggata gataaccagc
    19261 attcggagtc aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg
    19321 caatttatga atatcccgaa catttacatt cattccttaa tgacttaccc acccgaccag
    19381 gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta
    19441 atatccgcag tcgggtgctt tctcatttac gcaccccgga tgaagccgcc atgctacggc
    19501 aatcccggcg aattagctgg atctgcaccg caggcgaaat cggcgctctg ctccttgaag
    19561 cgcgattaat caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc
    19621 tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt
    19681 tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat
    19741 tgcagagcat cgctgatgaa caaaaacttt gttatggcct gttgggactg gaaccgttaa
    19801 gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta
    19861 aagagagcca tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgagtgg
    19921 tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca gcacccggaa atgactcaat
    19981 atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa
    20041 cgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc
    20101 tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt
    20161 gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc
    20221 gaaccagcac gcgccctggt cggtagtgag cagtttgtcg cccagttgcc acgccccgtt
    20281 aatcacaaac accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct
    20341 gactttcgcc ttacagacat ccaggcgcgt cataatattg aaatccatcg acatctgccc
    20401 tgccgtcagt ttcgctttta ccacctggtc cgccgtaaag gcaaaaggct gtaacggttt
    20461 taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt ccagcaacgt
    20521 cactatcctt tccatgccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat
    20581 gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc
    20641 accggcagcg tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact
    20701 cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatga ttatcggcaa
    20761 ctcgggtgat ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa
    20821 gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt
    20881 aagttttttg gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac
    20941 gttctgtcag acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcagga
    21001 tgttgtaaat tttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt
    21061 ccatttttgc gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt
    21121 catgcattgc gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca
    21181 tgatttcgtg gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt
    21241 cctgatgcgg accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg
    21301 cggtagtggt gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa
    21361 caaacagtgc agttaattta cgcatattct atatccttcc tttcagttat ttattacggc
    21421 tttcttaagt agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat
    21481 cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa
    21541 aaagaaaatt tgatggagag tgatgagaga atattacaac acgatgattt tgcagagatt
    21601 atgaagaact ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta
    21661 ttaatttata agaaagcaac ttaatacccg cagaatgatt tctgcgggta agtattagct
    21721 tattttttcg agcattaatc ccgcgcgtaa tcccaacgct accaacggat tagggaataa
    21781 cacatactct acatcatggg ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt
    21841 tcctttctca aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg
    21901 cgagtggcga gtaatttgcg aaaccacccg ataacggagc ggcggtgttc tcacgatacc
    21961 gacactctca ccagatagca gcgcagcaat tgcgctggca gttactgcaa actggcgaag
    22021 atcgttttgc ccaaagggca acgctttgcc aagttccaac gtacagacca gcgcgccaaa
    22081 atgtctgacg ctgaaatggg taaacgtacc accaggctcc tgatggaaca ccagcgcctc
    22141 cagccccgcc gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg
    22201 taatacaccg aactgcggat gcaaggagcc acgaattgcg gtatgtagat caaggtgcca
    22261 acgcacagat tctttgccct ggtcataaaa atcttccagg cactgttcca gttcgcgcgc
    22321 ccgacaggtt tctccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcggttcat
    22381 atcgctatgg caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag
    22441 caaccgccaa cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat
    22501 ttccacaggt gccgtctcat taccgtggat ccccgcagaa atcaccagcg cgccttgcgg
    22561 tggcgttaat ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc gaacgccgtt
    22621 gatttctcgc tcggtgataa ccggtttttt acccgttaag gtcagagcaa gaaaattatc
    22681 cattgccccc tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca
    22741 atacatccag tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag
    22801 taagacgatc gcggtagtaa cgatccaccc agtcattgag cacattaaac aaggtatcgt
    22861 tcatcatcac cgccggattc accgcccggc gttcttcttc tgtcaatacc ccccgcaacc
    22921 gcaggcacgc cgggccgccg ccattcgcca tgctttcacg taagtcaaag acttttagtt
    22981 cactaatcgg gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgct
    23041 cccgacactc ctgaggcagc accaacatca tggaaccatc atcgcggctc aacagttggc
    23101 tgttaaacag ataggtagac accgcatccg acacggaaac ctgagttgcc ggaacttcta
    23161 tcgccataaa accgttcacc cgcgtgccag taactgtgac tggcgagcga acgcctgttg
    23221 gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgctctg
    23281 gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattaag
    23341 ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt
    23401 atcattgcct tcttctcgcc cgtagacaaa aagtagcata cccggttcac cgtaatgacc
    23461 gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct gcggcaacgc
    23521 cgaatggacg ctaaatttct cttcgtcctt aaaaatcgct tttaacagcg attcagtgac
    23581 gggcgcttcc agcgaacggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt
    23641 gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga
    23701 agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg
    23761 ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg
    23821 cgggatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg
    23881 ttttgccgcc agtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc
    23941 aaacgacagg cccgcgtaat gatgcgtcag ccctaccagc ccgtcgaaat taacttccca
    24001 ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt ggcgggcaat
    24061 gttaacgagt ccgactccag gctcgccata ggccatgcgc agtaatctgc ggcataccag
    24121 gcgctggggc gatggttacc ggaagcacca atgccgccga atggggcggt actggcagca
    24181 ccagtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg
    24241 aatttttccc gctcggggga aaccagaccg caaaagagtc cgaagcgagt gttgttcgcc
    24301 atccgaatcg cctcatcaaa agtatcataa cgccagacgc gcaaaagcgg cccgaacacc
    24361 tcttcatccg gcacaccgcc aacgcccgtc atttcaatga tccctggtgt cagcaacgat
    24421 gtccctgctt gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc
    24481 tgccaggcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc
    24541 tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagactagca
    24601 agaaacgcat cgccctgcgc tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt
    24661 tgcccgactg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc
    24721 acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc
    24781 tgaccggaga gctggcgatg cagctggtag ccggtattgg cgctaccggt aaacagcaaa
    24841 ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg
    24901 ttcagcacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc
    24961 cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg
    25021 atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg
    25081 tgcgggcgat gtcgcaggct cgccgcaccg tccggcattt cactacgctg ctcgccggta
    25141 cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt
    25201 gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa ttcggcttta
    25261 ttgctttcca gcaatccggc aaagcgttcg acaacgacct gacgttcagc caatgagagc
    25321 cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgttcgac ctgagcggca
    25381 tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat
    25441 gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg
    25501 cgcacaggcg caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg
    25561 cggcggttaa aatcaaacgc tcggttgccg gatcggtacg cgccagcacc acgcggaaat
    25621 ggtgataatt ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt
    25681 ctgccacttc caccagccga cttttacgga tggcgcgcac gcggtcgatg tcacactcaa
    25741 gcgttggccc gccgtcgaag atgtcgatat agttgcggta acgaaaacct tctttctcca
    25801 gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct
    25861 gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt
    25921 gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga
    25981 ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca
    26041 ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca
    26101 aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc
    26161 tgccggtgtg atcgttacta agaaacagcg tcggcaatgc gttatagaca ttcagctctt
    26221 ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacct gggatcgttc agcccaaccg
    26281 ccacctcaat agcgcaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca
    26341 catagccctg ctcgcttttg ggcagttcac cttgccaggt tttgattgcc ctttcgatac
    26401 gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg
    26461 caagctgcat cagcgccgag acatctgagc gctcaacggg acggatgacc atcatgatga
    26521 acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc
    26581 ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacccg caatcagtac
    26641 catcacgcct gctttcgccg cttccagaga gatctgtttc gcttgcccgg cgtaatcggc
    26701 attcagtaca cagccaatca acaaacctaa gccgcgaact tcactgaaca aaccatagcg
    26761 gtgattaata gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag
    26821 catctctggt gtgttgatga gctccagcac tttgcctgct accgccgagg ccagcggatt
    26881 accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc
    26941 caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtggtta acagatcggg
    27001 cgtcacgccg tagtgcatat aggcatacag ctcaccagtg cgcccgacgc cggtttgtac
    27061 ctcatcaaaa atcagcaagg cattgtggcg gtcacacaat tcacgcagac cttgtaaaaa
    27121 cgcgttgctg gctggcacca caccgccttc gccctagatg ggctcgacaa tcaccgcaca
    27181 ggtggcgtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat
    27241 atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt
    27301 aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccactct tatggctgcc
    27361 gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc
    27421 ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg
    27481 cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt
    27541 cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc
    27601 gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg
    27661 tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat
    27721 tggctgagac atagcgacct ctacagtaaa taattcgtta tttgcatgtt aacaataagt
    27781 aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata
    27841 aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc
    27901 atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac gcaatgttat
    27961 gcagaagtaa aatataattc tagaattgtg atcattgacg aaatttactg gaaattactg
    28021 cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatca ttgacatcat
    28081 taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc
    28141 atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat
    28201 caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga
    28261 tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc
    28321 gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct
    28381 gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca
    28441 gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg
    28501 ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt
    28561 ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat
    28621 tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg
    28681 taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga
    28741 caggctgatg agctgggggg tggtcgatac cttccgccat gctaatccgc aaacagcaga
    28801 tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat
    28861 cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta
    28921 tgaaattcgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg
    28981 ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca
    29041 acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt
    29101 agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg
    29161 agcgtaactt tctgtttgcc tgtcttattt ttgcgctggt catttacgct atccacgctt
    29221 tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatt cgccagagcg
    29281 gattgttcgg ttatagcctc tatattctgt tattcatcaa tgccccctct ttctgttacc
    29341 aggaagcata ctggtgatcg ctggaggaat agtttttggc ccgctcttag gacacaactc
    29401 tcattaattg ccgcaacgct ggcctcctcg tgctcattcc tgctggcgcg ctggctggga
    29461 cgtgatttac tgctgaaata cgtgggccat agccatacct tccaggccat tgaaaaaggc
    29521 attgcgcgta acggtattga ttttcttatt ctgacccgct taatcccgtt gtttccttac
    29581 aatattcaaa attacgctta cggattcacc accatcgcct tctggcctta tacccttatt
    29641 tcggcactca cgaccctgcc cggtattgtt atttataccg tgatggcaag cgatctcgcc
    29701 aatgaaggca ttacgctgcg ctttatttta caactctgtc tggcgggcct ggcgctgttt
    29761 attctcatcc agctcgcaaa actctacgcc cgacacaaac atgtggatct gtctgcttcg
    29821 cgccgcagcc cacttactca cccaaaaaat gaaggataga acgatgtcgc aacattattc
    29881 agtgtcatgg aaaaaaggac tggctgcact ctgcttactg gctgttgccg ggcttagcgg
    29941 ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac gacggacttt cgaacagcca
    30001 accgctgcgc gtcgatgcca ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg
    30061 tttcctcacc gacgacactc gtcacggtat tgtgtttaaa gatggctcca acggacataa
    30121 atcgctgttt atggcttatg cgcccccgaa agcattttat gaagccctga aagaggcagg
    30181 tggtacgccg ggcgaaaaca tgacgatgga taataaagaa acgactcatg tcacaggcag
    30241 caaactggat atttcggtca actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt
    30301 gattgttgac agtaatggca agaaactgga catgcgcttt ggcggtaatt taacggcagc
    30361 agaagagaag aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag
    30421 caatgcaaca tacacttatg gtgcggtgga aaaacacggc gaagtgaaat tcaaaggcaa
    30481 tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt acctttaaaa tcaccgaata
    30541 aagccaagat aaaggatgat gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc
    30601 ctcatcatcc tgttattcgc tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag
    30661 tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt
    30721 attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttacaggcc
    30781 attgctgcac cgctacctgc gtttttgatc acctttgcca atgcgtcgct gtttggcgcg
    30841 ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt
    30901 atcgccagag tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac
    30961 agtatggacg gctttttcac tcgctacggc aaacacacca tcctggtctg tcggttattg
    31021 ccttttgtcc ctttcgatcc aatcagc
//
LOCUS       DAJVRL010000043        30338 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVRL010000043 DAJVRL010000000
VERSION     DAJVRL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.043"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OQN02_001937"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OQN02_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1642736.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OQN02_001938"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OQN02_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1642737.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OQN02_001939"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OQN02_001939"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1642738.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OQN02_001940"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OQN02_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1642739.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OQN02_001941"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OQN02_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1642740.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OQN02_001942"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OQN02_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1642741.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OQN02_001943"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OQN02_001943"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1642742.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OQN02_001944"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OQN02_001944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1642743.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OQN02_001945"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OQN02_001945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1642744.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OQN02_001946"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OQN02_001946"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1642745.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OQN02_001947"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OQN02_001947"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1642746.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OQN02_001948"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OQN02_001948"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1642747.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OQN02_001949"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OQN02_001949"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1642748.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OQN02_001950"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OQN02_001950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1642749.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OQN02_001951"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OQN02_001951"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1642750.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OQN02_001952"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OQN02_001952"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1642751.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OQN02_001953"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OQN02_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1642752.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OQN02_001954"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OQN02_001954"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1642753.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19506..20327
                     /locus_tag="OQN02_001955"
     CDS             19506..20327
                     /locus_tag="OQN02_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCS1642754.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20314..22422
                     /locus_tag="OQN02_001956"
     CDS             20314..22422
                     /locus_tag="OQN02_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS1642755.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22419..24086
                     /locus_tag="OQN02_001957"
     CDS             22419..24086
                     /locus_tag="OQN02_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1642756.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24089..25615
                     /locus_tag="OQN02_001958"
     CDS             24089..25615
                     /locus_tag="OQN02_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCS1642757.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25616..27232
                     /locus_tag="OQN02_001959"
     CDS             25616..27232
                     /locus_tag="OQN02_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS1642758.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27288..27440)
                     /locus_tag="OQN02_001960"
                     /pseudo
     CDS             complement(27288..27440)
                     /locus_tag="OQN02_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27463..27840)
                     /locus_tag="OQN02_001961"
     CDS             complement(27463..27840)
                     /locus_tag="OQN02_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642759.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28250..28621)
                     /locus_tag="OQN02_001962"
     CDS             complement(28250..28621)
                     /locus_tag="OQN02_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642760.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28682..29179)
                     /locus_tag="OQN02_001963"
     CDS             complement(28682..29179)
                     /locus_tag="OQN02_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642761.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29255..30043)
                     /gene="aadA1"
                     /locus_tag="OQN02_001964"
     CDS             complement(29255..30043)
                     /gene="aadA1"
                     /locus_tag="OQN02_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1642762.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(30101..>30338)
                     /locus_tag="OQN02_001965"
     CDS             complement(30101..>30338)
                     /locus_tag="OQN02_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1642763.1"
                     /translation="GKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGF
                     TLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
BASE COUNT         7796 a         7125 c         7803 g         7614 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc ttgtgggcgg
    19381 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
    19441 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
    19501 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
    19561 aggggcgtgg ccaagggcat ggtaaagact atattccatg gctaacagta caagaagttc
    19621 cttcttcagg tcgttcccac cgtatttatt ctcataagac gggacgagtc catcatttgc
    19681 tatctgactt agagcttgct gtttttctca gtcttgagtg ggagagcagc gtgctagata
    19741 tacgcgagca gttcccctta ttacctagtg ataccaggca gattgcaata gatagtggta
    19801 ttaagcatcc tgttattcgt ggtgtagatc aggttatgtc tactgatttt ttagtggact
    19861 gcaaagatgg tccttttgag cagtttgcta ttcaagtcaa acctgcagca gccttacaag
    19921 acgagcgtac cttagaaaaa ctagaactag agcgtcgcta ttggcagcaa aagcaaattc
    19981 cttggttcat ttttactgat aaagaaataa atcccgtagt aaaagaaaat attgaatggc
    20041 tttattcagt gaaaacagaa gaagtttctg cggagctttt agcacaacta tccccattgg
    20101 cccatatcct gcaagaaaaa ggagatgaaa acattatcaa tgtctgtaag caggttgata
    20161 ttgcttatga tttggagtta ggcaaaacat tgagtgagat acgagcctta accgcaaatg
    20221 gttttattaa gttcaatatt tataagtctt tcagggcaaa taagtgtgca gatctctgta
    20281 ttagccaagt agtgaatatg gaggagttgc gctatgtggc aaattaatga ggttgtgcta
    20341 tttgataatg atccgtatcg cattttggct atagaggatg gccaagttgt ctggatgcaa
    20401 ataagcgctg ataaaggagt tccacaagct agggctgagt tgttgctaat gcagtattta
    20461 gatgaaggcc gcttagttag aactgatgac ccttatgtac atcttgattt agaagagccg
    20521 tctgtagatt ctgtcagctt ccagaagcgc gaggaggatt atcgaaaaat tcttcctatt
    20581 attaatagta aggatcgttt cgaccctaaa gtcagaagcg aactcgttga gcatgtggtc
    20641 caagaacata aggttactaa ggctacagtt tataagttgt tacgccgtta ctggcagcgt
    20701 ggtcaaacgc ctaatgcatt aattcctgac tacaaaaaca gcggtgcacc aggggaaaga
    20761 cgttcagcga caggaacagc aaagattggc cgagccagag aatatggtaa gggtgaagga
    20821 accaaggtaa cgcccgagat tgaacgcctt tttaggttga ccatagaaaa gcacctgtta
    20881 aatcaaaaag gtacaaagac caccgttgcc tatagacgat ttgtggactt gtttgctcag
    20941 tattttcctc gcattcccca agaggattac ccaacactac gtcagtttcg ttatttttat
    21001 gatcgagaat accctaaagc tcagcgctta aagtctagag ttaaagcagg ggtatataaa
    21061 aaagacgtac gacccttaag tagtacagcc acttctcagg cgttaggccc tgggagtcgt
    21121 tatgagattg atgccacgat tgctgatatt tatttagtgg atcatcatga tcgccaaaaa
    21181 atcataggaa gaccaacgct ttacattgtg attgatgtgt ttagtcggat gatcacgggc
    21241 ttttatatcg gctttgaaaa tccgtcttat gtggtggcga tgcaggcttt tgtaaatgct
    21301 tgctctgaca aaacggccat ttgtgcccag catgatattg agattagtag ctcagactgg
    21361 ccgtgtgtag gtttgccaga tgtgttgcta gcggaccgtg gcgaattaat gagtcatcag
    21421 gtcgaagcct tagtttctag ttttaatgtg cgagtggaaa gtgctccacc tagacgtggc
    21481 gatgctaaag gcatagtgga aagcactttt agaacactac aagccgagtt taagtccttt
    21541 gcacctggca ttgtagaggg cagtcggatc aaaagccatg gtgaaacaga ctataggtta
    21601 gatgcatctc tgtcggtatt tgagttcaca caaattattt tgcgtacgat cttattcaga
    21661 aataaccatc tggtgatgga taaatacgat cgagatgctg attttcctac agatttaccg
    21721 tctattcctg tccagctatg gcaatggggt atgcagcatc gtacaggtag tttaagggct
    21781 gtggagcaag agcagttgcg agtagcgtta ctgcctcgcc gaaaggtctc tatttcttca
    21841 tttggcgtta atttgtgggg tttgtattac tcggggtcag agattctgcg tgagggttgg
    21901 ttgcagcgga gcactgatat agctagacct caacatttag aagcggctta tgacccagtg
    21961 ctggttgata cgatttattt gtttccgcaa gttggcagcc gtgtattttg gcgctgtaat
    22021 ctgacggaac gtagtcggca gtttaaaggt ctctcatttt gggaggtttg ggatatacaa
    22081 gcacaagaaa aacacaataa agccaatgcg aagcaggatg agttaactaa acgcagggag
    22141 cttgaggcgt ttattcagca aaccattcag aaagcgaata agttaacgcc cagtactact
    22201 gagcccaaat caacacgcat taagcagatt aaaactaata aaaaagaagc cgtgacctcg
    22261 gagcgtaaaa aacgtgcgga gcatttgaag ccaagctctt caggtgatga ggctaaagtt
    22321 attcctttca acgcagtgga agcggatgat caagaagatt acagcctacc cacatacgtg
    22381 cctgaattat ttcaggatcc accagaaaag gatgagtcat gagtgctacc cggattcaag
    22441 cagtttatcg tgatacgggg gtagaggctt atcgtgataa tccttttatc gaggccttac
    22501 caccattaca agagtcagtg aatagtgctg catcactgaa atcctcttta cagcttactt
    22561 cctctgactt gcaaaagtcc cgtgttatca gagctcatac catttgtcgt attccagatg
    22621 actattttca gccattaggt acgcatttgc tactaagtga gcgtatttcg gtcatgattc
    22681 gaggtggcta cgtaggcaga aatcctaaaa caggagattt acaaaagcat ttacaaaatg
    22741 gttatgagcg tgttcaaacg ggagagttgg agacatttcg ctttgaggag gcacgatcta
    22801 cggcacaaag cttattgtta attggttgtt ctggtagtgg gaagacgacc tctcttcatc
    22861 gtattctagc cacgtatcct caggtgattt accatcgtga actcaatgta gagcaggtgg
    22921 tgtatttgaa aatagactgc tcgcataatg gttcgctaaa agaaatctgc ttgaattttt
    22981 tcagagcgtt ggatcgagcc ttgggctcga actatgagcg tcgttatggc ttaaaacgtc
    23041 atggtataga aaccatgttg gctttgatgt cgcaaatagc caatgcacat gctttagggt
    23101 tgttggttat tgatgaaatt cagcatttaa gccgctctcg ttcgggtgga tctcaagaga
    23161 tgctgaactt ttttgtgacg atggtgaata ttattggcgt accagtgatg ttgattggta
    23221 cccctaaagc acgagagatt tttgaggctg atttgcggtc tgcacgtaga ggggcagggt
    23281 ttggagctat attctgggat cctatacaac aaacgcaacg tggaaagccc aatcaagagt
    23341 ggatcgcttt tacggataat ctttggcaat tacagctttt acaacgcaaa gatgcgctgt
    23401 tatcggatga ggtccgtgat gtgtggtatg agctaagcca aggagtgatg gacattgtag
    23461 taaaactttt tgtactcgct cagctccgtg cgctagcttt aggcaatgag cgtattaccg
    23521 ctggtttatt gcggcaagtg tatcaagatg agttaaagcc tgtgcacccc atgctagagg
    23581 cattacgctc gggtatccca gaacgcattg ctcgttattc tgatctagtc gttcccgaga
    23641 ttgataaacg gttaatccaa cttcagctag atatcgcagc gatacaagaa caaacaccag
    23701 aagaaaaagc ccttcaagag ttagataccg aagatcagcg tcatttatat ctgatgctga
    23761 aagaggatta cgattcaagc ctgttaattc ccactattaa aaaagcgttt agccagaatc
    23821 caacgatgac aagacaaaag ttactgcctc ttgttttgca gtggttgatg gaaggcgaaa
    23881 cggtagtgtc agaactagaa aagccctcca agagtaaaaa ggtttcggct ataaaggtag
    23941 tcaagcccag cgactgggat agcttgcctg atacggattt acgttatatc tattcacaac
    24001 gccaacctga aaaaaccatg catgaacggt taaaagggaa aggggtaata gtggatatgg
    24061 cgagcttatt taaacaagca ggttagccat gagaaacttt cctgttccgt actcgaatga
    24121 gctgatttat agcactattg cacgggcagg cgtttatcaa gggattgtta gtcctaagca
    24181 gctgttggat gaggtgtatg gcaaccgcaa ggtggtcgct accttaggtc tgccctcgca
    24241 tttaggtgtg atagcaagac atctacatca aacaggacgt tacgctgttc agcagcttat
    24301 ttatgagcat accttattcc ctttatatgc tccgtttgta ggcaaggagc gccgagacga
    24361 agctattcgg ttaatggagt accaagcgca aggtgcggtg catttaatgc taggagtcgc
    24421 tgcttctaga gttaagagcg ataaccgctt tagatactgc cctgattgcg ttgctcttca
    24481 gctaaatagg tatggggaag ccttttggca acgagattgg tatttgcccg ctttgccata
    24541 ttgtccaaaa cacggtgctt tagtcttctt tgatagagct gtagatgatc accgacatca
    24601 attttgggct ttgggtcata ctgagctgct ttcagactac cccaaagact ccctatctca
    24661 attaacagca ctagctgctt atatagcccc tctgttagat gctccacgag cgcaagagct
    24721 ttccccaagc cttgagcagt ggacgctgtt ttatcagcgc ttagcgcagg atctagggct
    24781 aaccaaaagc aagcacattc gtcatgactt ggtggcggag agagtgaggc agacttttag
    24841 tgatgaggca ctagagaaac tggatttaaa gttggcagag aacaaggaca cgtgttggct
    24901 gaaaagtata ttccgtaagc atagaaaagc ctttagttat ttacagcata gtattgtgtg
    24961 gcaagcctta ttgccaaaac taacggttat agaagcgcta cagcaggcaa gtgctcttac
    25021 tgagcactct ataacgacaa gacctgttag ccagtctgtg caacctaact ctgaagattt
    25081 atctgttaag cataaagact ggcagcaact agtgcataaa taccaaggaa ttaaggcggc
    25141 aagacagtct ttagagggtg gggtgctata cgcttggctt taccgacatg acagggattg
    25201 gctagttcac tggaatcaac agcatcaaca agagcgtctg gcacccgccc ctagagttga
    25261 ttggaaccaa agagatcgaa ttgctgtacg acaactatta agaatcataa agcgtctaga
    25321 tagtagcctt gatcacccaa gagcgacatc gagctggctg ttaaagcaaa ctcctaacgg
    25381 aacctctctt gcaaaaaatc tacagaaact gcctttggta gcgctttgct taaagcgtta
    25441 ctcagagagt gtggaagatt atcaaattag acggattagc caagctttta ttaagcttaa
    25501 acaggaagat gttgagctta ggcgctggcg attattaaga agtgcaacgt tatctaaaga
    25561 gcggataact gaggaagcac aaagattctt ggaaatggtt tatggggaag agtgagtggt
    25621 taggctagct acatttaatg acaatgtgca ggttgtacat attggtcatt tattccgtaa
    25681 ctcgggtcat aaggagtggc gtatttttgt ttggtttaat ccaatgcaag aacggaaatg
    25741 gactcgattt actcatttgc ctttattaag tcgagctaag gtggttaaca gtacaacaaa
    25801 gcaaataaat aaggcggatc gtgtgattga gtttgaagca tcggatcttc aacgagccaa
    25861 aataatcgat tttcctaatc tctcgtcctt tgcttccgta cgcaacaagg atggagcgca
    25921 gagttcattt atttacgaag ctgaaacacc atatagcaag actcgttatc acatcccaca
    25981 gttagagcta gctcggtcat tatttttaat taactcctat ttctgtcgaa gctgtttgag
    26041 cagtaccgct ttacagcaag agttcgacgt tcagtatgag gttgagcgag atcatttaga
    26101 gataaggatc ttacccagtt catcgtttcc taaaggggcg ttagagcagt cggccgtagt
    26161 gcagcttttg gtttggttgt tttcggatca agatgttatg gattcgtatg aaagtatttt
    26221 taggcactat caacaaaata gagaaattaa gaacggcgtt gaaagctggt gctttagctt
    26281 tgaccctccg cccatgcagg gttggaaatt acatgtaaaa ggacgttctt ctaacgagga
    26341 taaggattat ttagttgagg aaatagtagg tttagaaatc aacgctatgc ttcctagcac
    26401 aacagctatt agccatgcct cttttcagga aaaggaggca ggtgatggta gtacgcagca
    26461 catagcggtt tcaacagagt cagttgttga tgatgagcat ctacagttgg acgatgagga
    26521 aacagccaat atagacacag acacacgagt catagaggct gagccgacat ggataagttt
    26581 tagtagacct agtcgaattg aaaaatctcg cagggcaaga aaaagtagcc aaactatttt
    26641 agaaaaagaa gaagcaacaa caagtgaaaa tagtaatttg gttagtactg atgagccaca
    26701 cttaggtggt gtcctagcag cggcagatgt gggtgggaag caggatgcaa ccaattacaa
    26761 ctctattttt gctaatcgat ttgctgcttt tgatgagcta ctttcaattc taaaaactaa
    26821 atttgcatgt cgggtgcttt ttgaagaaac cttggttttg ccaaaagttg ggcgtagccg
    26881 attacatctg tgtaaagatg gctcaccaag agtgattaaa gccgttgggg tgcaacgtaa
    26941 tggcagtgaa tttgtattgc tagaggtgga tgcatcggat ggggtgaaaa tgctttctac
    27001 caaagtgttg agtggcgttg atagcgaaac atggcggaat gattttgaaa agatacggcg
    27061 tggagtggtg aagagctcat tgaattggcc aaatagtttg tttgatcaat tatatggaca
    27121 agacgggcat agaggggtga atcatccaaa ggggttgggg gagctgcaag tatcgagaga
    27181 ggatatggaa gggtgggctg agagagtggt tagagagcaa tttacgcatt aaaggaatga
    27241 ctgaaagagc ctgtaaaccc ttttgtgtaa gtgcttttgc cggtcagtta aaggtggcca
    27301 tttaaacggt caccaaattc gatcataaaa cggttcatgg ccggcttcca gttgcggatc
    27361 ggcatcgtcc atttcttggt cgccgcctgg atagccaggt acaccacctt catcgctgat
    27421 tcgtccgtag ggaacacctt gagttcatat taatggaatt ttctacaaat agcctccgtg
    27481 gttttgaggg gggattacag acgatccata gtagtaatcc aatgagttct tgagcgcggc
    27541 gacatgtttg gacgccttgg caaaaattag agcctgcttg aagtgcaggc gagcacgtgc
    27601 ttggacgatt gatccattcg cggtcaaaaa ctcaatcttg gatgacagcg tgtcaggaaa
    27661 tccaatatcg aagtcccgcc ccgtgtcaaa gtagggcatt tcatgatcaa aggacgaagc
    27721 ttcccaggcc acttccctaa tattccagcc gctagaaaca agatttcgta cgacttcatc
    27781 aacttttttg ctttccaact tgtcacaaag tgcataggca agagctttgg cggcagacac
    27841 gtgcgtttcc tcttaaacat aggtcgaacg gatacccgag gcaaagcgga gcattagtcc
    27901 caaagcatct ccggtagatc cggaggctcc atactgaagg catggcatct tgggcattga
    27961 tatttgaaac ttctctctag atagcaggga tttcttggcc ttgcctcatg ctgatagctt
    28021 gtaaatatca cacgatcctt agccccacaa agtgagcata ctccgtagct ttcatttata
    28081 gaaatcgtcg ttagattgtc gcagcatgtg cacgttccga ctatttgctc aggaagcatc
    28141 gccatacctt ggcctaggta aagaagatca gactcgtagc cacatcggtg gcaactgagc
    28201 ttgtacgcaa tgcccatctg gttgtctcac ctgaatcgta acgtcagagc tattcatcca
    28261 tccattcggc atcaatgtat gccactaagc cgaccagctg accgtttgga ctgaagtatg
    28321 ttgcctgcac cccacttgac atctcatcaa taccaccgca ctgcgctagg cgccggtccc
    28381 acttgcccac agaagcgtca ggatcggctg agttccccaa gacccttgtg agccatgcct
    28441 cctttaactc aacctctcga atgattgcgt cggccagctc ctccgcgtta tctagttcga
    28501 catagtctgg aattcggacg tacgttctga acacagctgg atacttactt gggcgattgt
    28561 catacatgac atcaacaatg tacccgtttg tgtaaccgtc tcttggaggt tcgtatgaca
    28621 ctagtggttc ccctcagctt gcgactagat gttgaggcct aacattttat tagagagcag
    28681 gctagttgct tagatacatg atcttcaggc cgttatctgt cagggcaagc gaaaattggc
    28741 catttatgac gaccaatgcc ccgcagaagc tcccatcttt gccgccatag acgccgcgcc
    28801 ccccttttgg ggtgtagaac atccttttgc cagatgtgga aaagaagttc gttgtcccat
    28861 tgttggcaat gacgtagtag ccggcgaaag tgcgagaccc atttgcgcta tatataagcc
    28921 tacgatttcc gttgcgacta ttgtcgtaat tggatgaact attatcgtag ttgctctcag
    28981 agttgtcgta atttgatgga ctattgtcgt aattgcttat ggagttgtcg tagttgcttg
    29041 gagaaatgtc gtagttggat ggggagtagt catagggaag acgagcttca tccactaaaa
    29101 caattggcag gtcagcaagt gcctgccccg atgccatcgc aagtacgagg cttagaacca
    29161 ccttcaacag atcgcgcata gtcttcccca gctctctaac gcttgagtta agccgcgccg
    29221 cgaagcggcg tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct
    29281 cgcctttcac gtagtgaaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
    29341 cttgtccaag ataagcctgc ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
    29401 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
    29461 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
    29521 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
    29581 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
    29641 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
    29701 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
    29761 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
    29821 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
    29881 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
    29941 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
    30001 ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt aactcctgaa
    30061 ttaagccgcg ccgcgaagcg gtgtcggctt gaatgaattg ttaggcgtca tcctgtgctc
    30121 ccgagaacca gtaccagtac atcgctgttt cgttcgagac ttgaggtcta gttttatacg
    30181 tgaacaggtc aatgccgccg agagtaaagc cacattttgc gtacaaattg caggcaggta
    30241 cattgttcgt ttgtgtctct aatcgtatgc caaggagctg tctgcttagt gcccactttt
    30301 tcgcaaattc gatgagactg tgcgcgactc ctttgcct
//
LOCUS       DAJVRL010000044        30096 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVRL010000044 DAJVRL010000000
VERSION     DAJVRL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.044"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OQN02_001966"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OQN02_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1642764.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OQN02_001967"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OQN02_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1642765.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OQN02_001968"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OQN02_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1642766.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OQN02_001969"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OQN02_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1642767.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OQN02_001970"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OQN02_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1642768.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OQN02_001971"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OQN02_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1642769.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OQN02_001972"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OQN02_001972"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1642770.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OQN02_001973"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OQN02_001973"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1642771.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQN02_001974"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQN02_001974"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1642772.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQN02_001975"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQN02_001975"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1642773.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQN02_001976"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQN02_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1642774.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQN02_001977"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQN02_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS1642775.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQN02_001978"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQN02_001978"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1642776.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQN02_001979"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQN02_001979"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1642777.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQN02_001980"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQN02_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1642778.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OQN02_001981"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OQN02_001981"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1642779.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OQN02_001982"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OQN02_001982"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1642780.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OQN02_001983"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OQN02_001983"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1642781.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OQN02_001984"
     CDS             21622..22212
                     /locus_tag="OQN02_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1642782.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OQN02_001985"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OQN02_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1642783.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OQN02_001986"
     CDS             22684..23379
                     /locus_tag="OQN02_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1642784.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OQN02_001987"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OQN02_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1642785.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OQN02_001988"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OQN02_001988"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1642786.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OQN02_001989"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OQN02_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS1642787.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OQN02_001990"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OQN02_001990"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1642788.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OQN02_001991"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OQN02_001991"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1642789.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7056 a         7842 c         8277 g         6921 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgattcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacttg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ttggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatgtcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa gagaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
    30061 tacgccacct cgcgacagtg cgctaaagcg cgtcgc
//
LOCUS       DAJVRL010000045        29797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVRL010000045 DAJVRL010000000
VERSION     DAJVRL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.045"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            56..850
                     /locus_tag="OQN02_001992"
     CDS             56..850
                     /locus_tag="OQN02_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1642790.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="OQN02_001993"
     CDS             872..2281
                     /locus_tag="OQN02_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1642791.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="OQN02_001994"
     CDS             2259..3038
                     /locus_tag="OQN02_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1642792.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3035..3895
                     /locus_tag="OQN02_001995"
     CDS             3035..3895
                     /locus_tag="OQN02_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1642793.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="OQN02_001996"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="OQN02_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="OQN02_001997"
     CDS             complement(4635..4895)
                     /locus_tag="OQN02_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1642794.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="OQN02_001998"
     CDS             complement(4886..6157)
                     /locus_tag="OQN02_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1642795.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OQN02_001999"
     CDS             complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OQN02_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1642796.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="OQN02_002000"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="OQN02_002000"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1642797.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="OQN02_002001"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="OQN02_002001"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1642798.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="OQN02_002002"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="OQN02_002002"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1642799.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="OQN02_002003"
     CDS             11499..11651
                     /locus_tag="OQN02_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS1642800.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12936
                     /locus_tag="OQN02_002004"
                     /pseudo
     CDS             11845..12936
                     /locus_tag="OQN02_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OQN02_002005"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OQN02_002005"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1642801.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="OQN02_002006"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="OQN02_002006"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1642802.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="OQN02_002007"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="OQN02_002007"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1642803.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="OQN02_002008"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="OQN02_002008"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1642804.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="OQN02_002009"
     CDS             17363..17686
                     /locus_tag="OQN02_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1642805.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="OQN02_002010"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="OQN02_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1642806.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="OQN02_002011"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="OQN02_002011"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1642807.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="OQN02_002012"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="OQN02_002012"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1642808.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="OQN02_002013"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="OQN02_002013"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1642809.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="OQN02_002014"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="OQN02_002014"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1642810.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="OQN02_002015"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="OQN02_002015"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1642811.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21947..23086
                     /gene="nlpD"
                     /locus_tag="OQN02_002016"
     CDS             21947..23086
                     /gene="nlpD"
                     /locus_tag="OQN02_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1642812.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="OQN02_002017"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="OQN02_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1642813.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="OQN02_002018"
     CDS             complement(24261..24668)
                     /locus_tag="OQN02_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1642814.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="OQN02_002019"
     CDS             24815..25408
                     /locus_tag="OQN02_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCS1642815.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="OQN02_002020"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="OQN02_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..27191)
                     /locus_tag="OQN02_002021"
     CDS             complement(26628..27191)
                     /locus_tag="OQN02_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642816.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27356..27607
                     /locus_tag="OQN02_002022"
     CDS             27356..27607
                     /locus_tag="OQN02_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1642817.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27609..29705
                     /locus_tag="OQN02_002023"
     CDS             27609..29705
                     /locus_tag="OQN02_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1642818.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7300 a         7382 c         8058 g         7057 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggcgtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggctgc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacca gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacactccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ttggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcaattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacacccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggatcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt caggggcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccggataa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggct cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatggacaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctc gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaatgatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga tgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcacgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat taccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat gtgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    27181 gcttttctca ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa
    27241 taaaccataa aaacaccaaa gaacccttta cagggaatct ttggtatctt aatctttatc
    27301 catacggata tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag
    27361 acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga
    27421 taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact
    27481 gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg
    27541 tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc
    27601 gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag
    27661 ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta
    27721 gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa
    27781 cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt
    27841 ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag
    27901 catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc
    27961 gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg
    28021 gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc
    28081 tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta
    28141 ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg
    28201 ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca
    28261 gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag
    28321 cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg
    28381 aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga
    28441 aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg
    28501 actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga
    28561 aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg
    28621 gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg
    28681 ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata
    28741 acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc
    28801 tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac
    28861 gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg
    28921 gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc
    28981 tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt
    29041 gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt
    29101 ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac
    29161 gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt
    29221 tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt
    29281 actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc
    29341 atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat
    29401 ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc
    29461 gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta
    29521 ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg
    29581 gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac
    29641 gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg
    29701 cctgaataaa aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt
    29761 gaaataaccg aggaaatact atgactgata ttgatga
//
LOCUS       DAJVRL010000046        29636 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVRL010000046 DAJVRL010000000
VERSION     DAJVRL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.046"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..188)
                     /locus_tag="OQN02_002024"
     CDS             complement(<1..188)
                     /locus_tag="OQN02_002024"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCS1642819.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(329..2362)
                     /gene="metG"
                     /locus_tag="OQN02_002025"
     CDS             complement(329..2362)
                     /gene="metG"
                     /locus_tag="OQN02_002025"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1642820.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2494..3603
                     /gene="apbC"
                     /locus_tag="OQN02_002026"
     CDS             2494..3603
                     /gene="apbC"
                     /locus_tag="OQN02_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1642821.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3865..4146
                     /locus_tag="OQN02_002027"
     CDS             3865..4146
                     /locus_tag="OQN02_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS1642822.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4439..4963
                     /locus_tag="OQN02_002028"
     CDS             4439..4963
                     /locus_tag="OQN02_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1642823.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5044..5718
                     /locus_tag="OQN02_002029"
     CDS             5044..5718
                     /locus_tag="OQN02_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1642824.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5734..8214
                     /locus_tag="OQN02_002030"
                     /pseudo
     CDS             5734..8214
                     /locus_tag="OQN02_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8230..9263
                     /locus_tag="OQN02_002031"
                     /pseudo
     CDS             8230..9263
                     /locus_tag="OQN02_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="OQN02_002032"
     CDS             complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="OQN02_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1642825.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9902..10750)
                     /gene="rcnA"
                     /locus_tag="OQN02_002033"
                     /pseudo
     CDS             complement(9902..10750)
                     /gene="rcnA"
                     /locus_tag="OQN02_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10872..11144
                     /gene="rcnR"
                     /locus_tag="OQN02_002034"
     CDS             10872..11144
                     /gene="rcnR"
                     /locus_tag="OQN02_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCS1642826.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11236..11332
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11367..12155
                     /gene="thiM"
                     /locus_tag="OQN02_002035"
     CDS             11367..12155
                     /gene="thiM"
                     /locus_tag="OQN02_002035"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1642827.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12152..12952
                     /gene="thiD"
                     /locus_tag="OQN02_002036"
     CDS             12152..12952
                     /gene="thiD"
                     /locus_tag="OQN02_002036"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1642828.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13017..13838
                     /locus_tag="OQN02_002037"
     CDS             13017..13838
                     /locus_tag="OQN02_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1642829.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13886..14632
                     /locus_tag="OQN02_002038"
     CDS             13886..14632
                     /locus_tag="OQN02_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1642830.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14606..15571)
                     /locus_tag="OQN02_002039"
     CDS             complement(14606..15571)
                     /locus_tag="OQN02_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1642831.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGDDCAPTREELLLAHKNV"
     gene            complement(15568..16571)
                     /locus_tag="OQN02_002040"
                     /pseudo
     CDS             complement(15568..16571)
                     /locus_tag="OQN02_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16568..17845)
                     /locus_tag="OQN02_002041"
     CDS             complement(16568..17845)
                     /locus_tag="OQN02_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1642832.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18102..19154
                     /gene="fbaB"
                     /locus_tag="OQN02_002042"
     CDS             18102..19154
                     /gene="fbaB"
                     /locus_tag="OQN02_002042"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1642833.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19462..20316
                     /gene="gatY"
                     /locus_tag="OQN02_002043"
     CDS             19462..20316
                     /gene="gatY"
                     /locus_tag="OQN02_002043"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1642834.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20345..21607
                     /gene="gatZ"
                     /locus_tag="OQN02_002044"
     CDS             20345..21607
                     /gene="gatZ"
                     /locus_tag="OQN02_002044"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCS1642835.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21617..22069
                     /gene="gatA"
                     /locus_tag="OQN02_002045"
     CDS             21617..22069
                     /gene="gatA"
                     /locus_tag="OQN02_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1642836.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22100..22384
                     /gene="gatB"
                     /locus_tag="OQN02_002046"
     CDS             22100..22384
                     /gene="gatB"
                     /locus_tag="OQN02_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1642837.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22388..23743
                     /locus_tag="OQN02_002047"
     CDS             22388..23743
                     /locus_tag="OQN02_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1642838.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23791..24831
                     /gene="gatD"
                     /locus_tag="OQN02_002048"
     CDS             23791..24831
                     /gene="gatD"
                     /locus_tag="OQN02_002048"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1642839.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24937..25710
                     /locus_tag="OQN02_002049"
     CDS             24937..25710
                     /locus_tag="OQN02_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1642840.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25792..26691)
                     /gene="yegS"
                     /locus_tag="OQN02_002050"
     CDS             complement(25792..26691)
                     /gene="yegS"
                     /locus_tag="OQN02_002050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS1642841.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27097..27414
                     /locus_tag="OQN02_002051"
     CDS             27097..27414
                     /locus_tag="OQN02_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642842.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27756..29117)
                     /gene="yegQ"
                     /locus_tag="OQN02_002052"
     CDS             complement(27756..29117)
                     /gene="yegQ"
                     /locus_tag="OQN02_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1642843.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29264..29595)
                     /locus_tag="OQN02_002053"
                     /pseudo
     CDS             complement(29264..29595)
                     /locus_tag="OQN02_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7700 a         6878 c         7477 g         7581 t
ORIGIN      
        1 cccttcttca ccttctccgc aatcagcttc agttccgctt tttccgccgc cgcagcatct
       61 gaaaaacttt ttatctgact ttgccctttg gtgccaactt ttccccaact gatatgcaac
      121 tcgtttccct gttgctccac cgtccagaat ttatgtgatt tttcgtcctg atagataaag
      181 tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac cagtatttat
      241 acgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc
      301 gctgtcgatg cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg
      361 catccgggct tagcaggaaa atatctttcc cgccaggacc ggcagccatc accatgcctt
      421 cagagatacc gaagcgcatt ttacgtggtg ccaggttagc caccataatg gtgtgacgac
      481 caatcagtgc ctgcggatcc gggtaagcag aacgaatacc ggagaagaca ttgcgttttt
      541 caccgccgag atccagcgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt
      601 tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct
      661 gaatcggatc atctgccagt gggccagtaa ctggcgcggt agtggctttc acttcttctt
      721 tagaggcttc caccagagct tcaacctgtt tcatatcgat gcggttatac agcgccttga
      781 acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc agttccgtat
      841 tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac cggcttcagg taagtcatca
      901 gcacgcggaa caggttgatg cccattgagc aaatcgcctg caggtcagca tcgcgacctt
      961 cctgttttgc gactacccac ggagcctgct cgtcgacata gcggttagcc aggtcagcca
     1021 gtgccatgat ttcgcgcact gctttaccaa attcacggct ttcccacgct tcaccaatca
     1081 cttcagcggc atcggtaaag gttttgtaca gctcagggtc agccagctcg cttgccagca
     1141 caccgtcaaa acgcttgttg ataaagcccg cattacggga ggccaggtta accactttgt
     1201 tcacgatatc ggcattcaca cgctgaacga aatcttccag gttgagatcg atatcatcaa
     1261 tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca
     1321 gccaggtgct ggctttaata aaggtgccgc gagacttgga catcttcgcg ccgttcaccg
     1381 tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg
     1441 caggccagaa caggctgtgg aagtaaacga tatctttacc gatgaagtgg tacagctcgg
     1501 cggtggagtc tttcttccag tattcatcga agcttacgct gtcgccgcgc ttgtcgcaca
     1561 gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc
     1621 ccggtgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt
     1681 gcaggccgga ttcaaaccac tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc
     1741 tgcgggtcca tgcctgcagc atttcgctga aagagggcag atcaaagaag aagtgttcag
     1801 aatcacgcat cactggtgta gcaccagaaa ccaccgattt cggctcgatc aattcagtcg
     1861 ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt
     1921 tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca
     1981 gctgagagat ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt
     2041 ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa
     2101 agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga
     2161 taccgagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga
     2221 agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct
     2281 ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa
     2341 ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat
     2401 agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag
     2461 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
     2521 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
     2581 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
     2641 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
     2701 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
     2761 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt
     2821 atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg
     2881 ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca
     2941 tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga cggtacccac
     3001 atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac
     3061 gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca gatgttgcag
     3121 gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac
     3181 attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg
     3241 caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt
     3301 ccggtgctcg gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat
     3361 gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca tacacagctg
     3421 ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg
     3481 gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt
     3541 gcagctcagc tctactggca gggtgaagtc attcctggcg agatttcctt ccgcgcggtc
     3601 taacgcctat taaccattct ggttattctt catacaccgc agagatgtta tattgatgcg
     3661 gtgtatttgt ttttgccgca ttaatgaaca cttatttaat actgcgaata ttctgcggac
     3721 cttcgtccga aactctacaa agtcgagtga tcgtacaggc aatgcttcat tatcatcctt
     3781 cgtgctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     3841 cctttactac agggataacg ttcaatgaat aagtattggc tgtcaggaat tatttttctt
     3901 gcctatggat tggcttctcc cgcagtctct tcagagactg ccacattaac gattaatggc
     3961 aggatcagtc ccccaacatg cagtatggct atggttaata gtcaaccaca acagcattgt
     4021 ggtcagttaa cgtataatgt tgacacccgt catcaggttt cttcgcccgt caaaggtgtt
     4081 acgacagaag tggttgttgc aggtagcgat agcaaacgtc gaatcgtgtt aaatcgctac
     4141 gattaatttg gcaacctgat tttaattgca tttagaaaga cgaaatcatt tatataaatg
     4201 atttcgtctt ttttattgat atcaacaata ccatttacat aaatctacat taattaatag
     4261 atgtgataaa tatttaatat tcttaagtat ttgttgtatt tatattgcat tggttaattt
     4321 gttttaaatc aataaaacaa tatgtttaca ttgcggcagc ttttttgatg tgagaaattt
     4381 gtttccaaga gatacagact cttaacaacc aaaaataaat tttattaagg aacatattat
     4441 gaaacgttca attattgctg ccgctgtctt ttcttctttt tttatgagcg ctggagtatt
     4501 tgctgcagac gttgataccg gaacattaac cattaaagga aatattgcag aatctccgtg
     4561 taaattcgaa gcgggtggtg attcagtaag tattaatatg ccgactgtac caaccactgt
     4621 ctttgaaggt aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagcagcat
     4681 gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa ctctcgttga tcacaaacga
     4741 caaaataacc ggtaacgata aggcgatagc cagtagcaac gataccgtag gtgataacag
     4801 cgatgtccta gatgtttctg caccttttaa cattgagagt tataaaacag cggaaggtca
     4861 atatgctatt ccgtttaaag caaaatacct gaaactgaca gataactcag tgcaatcagg
     4921 tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat taataaataa aattgcaagt
     4981 ttctccaggt aataaacatg gctgctatcc ccatggcggc tttttaattt aagaggcatt
     5041 aagatgaaag gattattatc tttactcatt ttttctatgg tccttcctgc acatgccgga
     5101 attgttatct acgggacgcg cattatttac ccggcagaaa ataaagaagt tatggtgcag
     5161 ttgatgaacc agggaaatcg ttcttcgctg ctgcaggcgt ggattgatga tggcgatacg
     5221 tcgttaccac cagaaaaaat tcaggttcct ttcatgttaa cgccaccagt ggcaaaaata
     5281 ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc cgaataaact gcccactaat
     5341 aaagaaagca ttttttatct gaatgttctg tatattccac caaatagtcc agagcaagaa
     5401 ggcaagaatg cactgaagtt tgcgatgcaa aacagaatta agttgtttta ccggccagcg
     5461 ggtattgctc cggtaaataa agcgaccttt aaaaagttac tggtaaatcg cagtggcaat
     5521 ggattggtga taaaaaatga ctcagctaat tgggtgacga tttcggatgt caaagctaat
     5581 aatgttaaag tcaattatga aactattatg attgccccct tagaaagtca gagtgttaat
     5641 atcaaaagta ataatgcaaa taactggtat ctgaccatta tcaatgacca cggcaactat
     5701 attagtgaca aaatttaatt gcaggagctg cctatgttga gaatgacccc acttgcatca
     5761 gcaatcgtag cgttattgct cggcattgaa gcttatgcag ctgaagaaac ctttgatacc
     5821 cattttatga taggtggaat gaaagaccag caggttgcaa atattcgtct tgatgataat
     5881 caacccttac cggggcagta tgacatcgat atttatgtca ataagcaatg gcgcgggaaa
     5941 tatgagatta ttgttaaaga caacccgcaa gaaacatgtt tatcaagaga agttatcaag
     6001 cggttaggca ttaatagcga taacttcgcc agcggtaagc aatgtttaac atttgagcaa
     6061 cttgttcagg gtgggagcta tagctgggat atcggggttt ttcgtctcga tttcagtgtc
     6121 ccgcaggctt gggtggaaga actggaaagt ggctatgttc caccggaaaa ctgggagcgg
     6181 ggtattaatg cgttttatac ttcttattat gtgagtcagt attacagcga ctataaagcg
     6241 tcgggtaaca acaagagtac atatgtacgt tttaacagcg ggttaaattt actggagtgg
     6301 caactgcatt ctgatgccag tttcagtaaa acaaataaca atccaggggt gtggaaaagc
     6361 aataccctgt atctggaacg tggatttgcc caatttctcg gcacgcttcg cgtgggtgat
     6421 atgtacacat caagcgatat ttttgattct gttcgcttca gcggtgtgcg gttgtttcgt
     6481 gatatgcaga tgttgcctaa ctcgaaacaa aattttacgc cacgggtgca ggggattgct
     6541 cagagtaacg cgctggtaac tattgaacag aatggttttg tggtttatca gaaagaggtt
     6601 cctcctggcc cgtttgcgat tacagatttg cagttggccg gtggtggagc agatcttgat
     6661 gttagcgtga aagaggcgga cggctcggta accacctatc tggtgcctta tgcagcggtg
     6721 ccaaatatgc tgcaacccgg cgtgtcgaaa tatgattttg cggcgggccg tagccatatt
     6781 gaaggggcga gcaagcaaag tgattttgtc caggcaggtt atcagtatgg ttttaataac
     6841 ttattgacgc tgtatggcgg cacgatggtt gctaataatt actatgcgtt taccctctga
     6901 acgggttgga acacgcgcat tggcgcaatt tccgtcgatg ccacgaagtc gcatagtaaa
     6961 caagacaacg gcgatgtgtt tgacgggcaa agttatcaaa ttgcctacaa caaatttgtg
     7021 agccaaacgt cgacgcgttt tggtctggcg gcctggcgtt attcgtcgcg tgattaccgg
     7081 acatttaacg atcacgtttg ggcaaacaat aaagataatt atcgccgtga tgaaaacgat
     7141 atctatgaca ttgccgatta ttaccagaac gattttggcc gtaaaaatag cttttctgcc
     7201 aatatgagcc agtcattgcc agaaggctgg ggttctgtgt ccttaagtac gttatggcga
     7261 gattactggg ggcgtagcgg cagcagtaag gattatcagt tgagttattc caataactgg
     7321 cggcggataa gctataccct cgcggcaagc caggcgtatg gcgagaatca tcatgaagag
     7381 aaacgtttta atatttttat atcaattccc tgtgattggg gtgatgacgt tacgacgcct
     7441 cgtcggcaaa tatatatgtc taactcaacg acgtttgatg atcagggttt tgcctcaaat
     7501 aatacgggat tatcaggaac agtagggagt cgggatcagt tcaattatgg tgtcaacctg
     7561 agtcatcaac atcagggaaa tgaaacgaca gctggggcga atttgacctg gaacgcgccg
     7621 gttgcgacag tgaatggcag ttatagtcag tcgagtactt atcgacagac tggagccagt
     7681 gtttcagggg gcattgtcgc ctggtcgggt ggcgttaatc tggcgaaccg tctttccgaa
     7741 acgtttgctg tgatgaatgc gccaggaatt aaagatgctt atgtcaatgg gcaaaaatat
     7801 cgcacaacaa accgtaatgg agtggtggta tacgacggaa tgacacctta tcgggaaaat
     7861 cacctgatgc tggatgtgtc gcaaagcgat agcgaagcag aattacgtgg caaccggaaa
     7921 attgccgccc cttatcgcgg cgcggttgta ctggttaatt ttgataccga tcagcgcaag
     7981 ccctggttta taaaagcgtt aagagcggat ggtcaaccat taacgtttgg ttatgaagtc
     8041 aatgatatcc atggtcataa tattggcgtt gtcggccagg gaagccagtt atttattcgc
     8101 accaatgaaa taccgccatc ggttaatgta gcaattgata agcaacaagg actttcatgc
     8161 acaatcacct tcggtaaaga gattgatgaa agtagaaatt atatttgcca gtaatagtca
     8221 ggtggttcta tggaaattcg cataatgcta tttatattaa tgatgatggt tatgcctgtg
     8281 agctatgcgg catgttatag tgagttatct gttcagcaca atttggttgt tcaggggaat
     8341 tttgcactta ctcaaacaca aatagcgaca tatgagcata attttaatga ttcgtcatgc
     8401 gtgagtacaa atactatcac ccctatgagc ccatcggata ttattgttgg actttataac
     8461 gataccatta aattaaattt acattttgaa tggaccaata aaaacaacat cacgttgtca
     8521 aataatcaga gcagtttcac cagtggttat tcggtaacgg tgacacctgc ggccagtaat
     8581 gcaaaagtga atatttctgc ggggggcggt ggttcagtga tgattaatgg tgttgcgaca
     8641 ttatccagtg cttcatcatc gacacgcggg agtgccgcag tacaatttct actgtgttta
     8701 ttaggtggca agtcatgggg tgcatgtgta aatagctaca gaaatgcatt ggcacaaaat
     8761 gcaggtgtct attccttcaa tctgacattg tcatacaacc cgataaccac aacctgcaaa
     8821 caggacgatt tattaattac tttagacagt attcccgttt cacaattacc agccacaggt
     8881 aacaaagcaa caataaatag taaaaaaagg gatattattc tgcgttgtaa aaatttatta
     8941 ggtcaacaaa atcaaacatc acggaaaatg caggtgtatt tatcaagttc tgacttgtta
     9001 accaacagca acacaatact gaaaggtgcg gaagataatg cgtaggattt attcttgaaa
     9061 gtaatggttc gccagtcaca cttttaaata tcactaacag cagtaaagga tacacaaatt
     9121 taaaagaaat tgcggcaaag tcaaaactta cagatacaac ggtttcaatt ccgataacag
     9181 ccagttacta cgtctacgat acaaacaaag ttaaatctgg cgcactggag gcaaccgcat
     9241 taatcaacgt gaaatacgac taataacaag caaaacgagc aagtggctaa taataaaaaa
     9301 taacaaggtg tctgcatttc cccctcatga tgagggggct ttttttagcg atgataaaaa
     9361 atctcaccgt cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa cacgtacgcg
     9421 ccacccatat aggtccagtg cgtcccggca tcaggcgcgg gcaggttacg taactgccac
     9481 tgcttaatgt tgtactcatc ggtgcggtac atctctggga cggtatcacc tggtttgaaa
     9541 tgcttaaagt cagcaatgaa actacttaac tcatacttag aaattcccga ggtattggtc
     9601 gaacccgcgg tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag cacacccagc
     9661 aacatcttat ttttaatggt cataaactct ccactttggg gatctgtaat atcagtcgca
     9721 tatctttcct gtcatacaat ataactgaca agcgatattt tgttagtgat cccatgattt
     9781 cgtaaaaagt gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag agagtcagcg
     9841 gaaatagctg atttaagaat catcctgggc tgaagataac atgcaagcct aaaggattga
     9901 gttatcgcat tatgcccatg aagccatgta cgcccatata cacaccgact aagccaatca
     9961 acagactgga aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc cagcgttttg
    10021 cgacctgctg aacgctgatt gctgcgccaa cgcctacggt gacaagcgtt aacgccaggc
    10081 caatgctgaa actgacgacc agtgttgcgc ccagtgtcag ggctttcaac tgaatgcata
    10141 tcaacagcac ggtaattgct gccgggcagg ggataaagcc accggttaag ccaaataaca
    10201 aaatttgcca gttgatgacc tctctaccat caaagcgtcg tttaatgtca ttggcatggg
    10261 ctcgtgcatg ggcatcctga tactcgccat attcgtgatg gtgatgatgt ccttggtcgt
    10321 ggtgatgttc gtggtcttga tgatgttcgt gatcgtgatg atggtgatga tgctcataat
    10381 catgcccgtg catattctcc agccagttgc gttcgccgcg ctaggtacgc cagaacatcc
    10441 agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt tctgctgatt
    10501 gagcagtaaa gcgcttactg atcaccatcc cgccaaaggc aatcaaccag accaccgcgg
    10561 tatgcgaaat ggttgctgcc agtccgagca tcaccgcctg tttaatggtg cctttgatgg
    10621 cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca tgaagcgcac
    10681 caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga agagttgtaa
    10741 attcggtcat gataataatt cttagtatta attcggcaat ctgattctac tcccccccag
    10801 tacctgatac taccccccca gtagattaat agtgctatga tttttcatat tcttgttaac
    10861 caggtgttgc catgtctcat acaatccgtg ataaacagaa actgaaagcg cgtgccagta
    10921 agattcaggg ccaggtcgtg gcgctcaaga aaatgctcga cgagccgcac gaatgcgctg
    10981 cagttttaca acagattgct gctatccgtg gagcggtaaa cggtctgatg cgggaagtga
    11041 ttaaaggtca tctgacggaa cacatcgttc accaggggga tgagctaaaa cgtgaagaag
    11101 atctggatgt cgttctgaag gtgctggatt catatatcaa ataatttatt aacgcgattg
    11161 taaaactgcc gtttttcctc gtttacaacg ggggcgctgg acattaccat cctcctctgc
    11221 gatttatcat cgcaaccaaa cgactcgggg tgcccttctg cgtgaaggct gagaaatacc
    11281 cgtatcacct gatctggata atgccagcgt agggaagtca cggaccacca ggtcattgct
    11341 tcttcacgtt atggcaggag caaactatgc aagtcgacct gctgagttca gcgcaatctg
    11401 cgcacgcgtt acaccttttt caccaacatt cccctcttgt gcactgcatg accaatgatg
    11461 tggtgcaaac ctttaccgcc aataccttgc tggcgctcgg tgcatcgcca gcgatggtta
    11521 tcgaaaccga agaggccagt cagtttgcgg ctatcgccag tgccttgttg attaacgttg
    11581 gcacactgac gcagccacgc gcacaggcga tgcgtgctgc cgttgagcaa gcaaaaagct
    11641 ctcaaacacg ctggacgctt gatccagtag cggttggtgc gctcgattat cgccgccatt
    11701 tttgtcatga acttttatct tttaaaccgg cagcgatacg tggtaatgct tcggaaatca
    11761 tggcattagc tggcattgct aatggcggac ggggagtgga taccactgac gccgcagcta
    11821 acgcgatacc cgctgcacaa acactggcac gggaaactgg cgcaatcgtc gtggtcactg
    11881 gcgaggtgga ttatgttacc gatggacatc gtatcattgg tattcacggt ggcgatccgt
    11941 taatgaccaa agtggtagga actggctgtg cattatcggc ggttgtcgct gcctgctgtg
    12001 cgttaccagg cgatacgctg gaaaatgtcg catctgcctg tcactggatg aaacaagccg
    12061 gagaacgcgc agtcgccaga agcgaggggc caggcagttt tgttccacat ttccttgatg
    12121 cgctctggca attgacgcag gaggtgcagg catgaaacga attaacgctc tgacgattgc
    12181 cggtactgat ccgagtggtg gtgcggggat tcaggccgat cttaaaacct tctcggcact
    12241 tggcgcttat ggttgctcag ttattactgc actggtggcg caaaataccc gtggcgtaca
    12301 gtcggtgtat cgcattgagc ctgattttgt cgccgcccag ctcgattcgg tgttcagcga
    12361 tgtgcgaatc gataccacta aaatcggtat gttggcggaa accgatattg ttgaagcggt
    12421 ggcagaacgg ttgcaacgtt atcagatcca aaacgtggta ctcgacaccg ttatgctggc
    12481 aaaaagcggc gacccgctgc tttcaccttc ggcggttgct acgctgcgca gtcgattatt
    12541 gccacaggtt tcattaataa cgccaaactt gcccgaagct gccgccttgc tcgacgcgcc
    12601 acacgcgcgc accgaacagg aaatgctgga acaagggcga tcgctgttgg cgatgggctg
    12661 tggcgcagtg ctaatgaaag gtggtcatct ggatgatgag caaagcccgg actggctgtt
    12721 tacccgcgag ggtgaacaac ggtttaccgc accgcgcatt atgaccaaaa acacccacgg
    12781 cactggttgt acactctctg cggcgttggc tgcactacgc ccgcgccata caaactgggc
    12841 tgacaccgta caggaggcaa aaatctggct ttcatcggcg ttagcccagg ccgacacgct
    12901 ggaagttggt cacggtattg gtccggttca ccacttccac gcctggtggt gagcaccgta
    12961 ctatagctgc tttggttatt gtcagccttt ggcactgaca ttcagggatg aaaccaatgc
    13021 agctaagaat taccagtcga aaaaaactca ccgctctatt atgcgctctt gggctaattt
    13081 ccatcgttgc aatttatccg cgtcagacgg tgaatttttt ctattcgaca gcagttcaga
    13141 ttacagacta catccacttc tacggttatc gcccggttaa atctttcgct attcgcattc
    13201 ctgccagtta caccattcac ggattagatg tttcgcgctg gcaggagcgt atcgactggc
    13261 agcgggtggc aaaaatgcgc gacaacgata tccgcttaca gtttgctttt attaaggcta
    13321 cggaaggcga aaagctggtg gacccctatt tttcgcgtaa ctggcaatta agccgcgaaa
    13381 atggcctgct gcgcggggcg tatcattatt tctccccgtc ggtatccgct tcagttcagg
    13441 cgcgattatt tctgcaaacg gtggatttct cacaaggcga tttccctgcc gtgctggacg
    13501 tagacgaacg gggaaaatta tcggcaaaag aattacgcaa gcgggtaagt cagtggctaa
    13561 aaatggtcga aaaacgcacg ggaaaaaagc cgattattta ctcaggagcc gttttttatc
    13621 acaccaatct ggcgggctat ttcaatgaat atccgtggtg ggtggctcac tattatcaac
    13681 gtcgtccgga caatgacggc atggcctggc gcttctggca gcattccgac cgtggacagg
    13741 tagatggcat caatggtccg gtggatttta atgtgtttaa tggcacgggg atgagttgca
    13801 ggcattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata tactcccacc
    13861 agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga ttgcccaact
    13921 gaacgagcgt attttagcgg cggataacac gccgctttat attaagtttg ccgaaacggt
    13981 aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg gtgagcgtga
    14041 tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc aggcgctgga
    14101 agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca acatcttcga
    14161 atactcgttg aaagaagcga gaggtttttc tcagcaggtg gtattgtgcg ggaaaaagcc
    14221 cgatacgcta tgggttaata agcgtgtcgt gaaatgtccc gaagaagttg cgcagcagct
    14281 ggcggtcgaa gcgggaagtg atgtcttttt gcttaagcgt attcgctatg tcgatgaaga
    14341 agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg ttgatgccat
    14401 cggaatttct ctttatgact atttccgcag ccagcacatt tacccacaac gtacgcgttc
    14461 ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt tagatagcaa
    14521 aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc ctattgagta
    14581 cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt agttcctcgc
    14641 gcgttggcgc acaatcatcg ccccgatgcc cgacaaccca cgacgccact gcattgccca
    14701 gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg ccacctgcat
    14761 gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga atgcagccag
    14821 aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc aacggcgcgg
    14881 caaatttctc ctgccattgc ttgccaagtg ttgttatctc tgcggataaa gcaaaacgtt
    14941 cggcggcaat ctcagcctct tgacgattga gtgacactaa aggtcgacag gccatgatcc
    15001 gcgccagtaa tgcgtccggg atatcgccaa tacgtgggca aaaatcgata aacggcgtca
    15061 cgtcttgcag cttttccagc cattccacta acaattcgcc gcagggcgag gccagttgat
    15121 aaccggaaaa atagagcagg ttgccaggcg caacggttaa tcgtgccaac cattggcgat
    15181 tccattgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc ggctcaacca
    15241 gtgccagaca ccagccgtta tcgctttcgg cgttatcgat caggctgatt aacccctctt
    15301 tcgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc aaggcattac
    15361 ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag ccgccaacgt
    15421 taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca taagcgtcgg
    15481 cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg gttaattcag
    15541 gcagcagtgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac gatatttcgc
    15601 cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagct ccgcctttaa
    15661 cgcaggatcg acagcgttaa tgccatgcaa cgcgccgcaa atcgccgtcg ccatagcacc
    15721 tatggtgtct gtgtcgccgc caagattggc gcacaggacg gcgcagcggt ttgggtcggt
    15781 ttgtgccagt tcaactagcg caatggcgca cggaacggac tcaatagtgc tggtgcctgc
    15841 gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat tacgcacaat
    15901 tttcagcgca atctccagac gtgccgccag tgaggcgctg aacgtggtgg tgcgtttttg
    15961 ctgtgcatgt cgcgcaattg aaggcaatga atcaacaatc gctgaccagc gttctccgtc
    16021 aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca gatcggattt
    16081 atgggtcggg ctggacgcca gcgccacatc atcaataaag gaatcaacat cacgcgctgg
    16141 aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggttacgc cgttgttttc
    16201 cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg aggtcggacc
    16261 tagtacgttt ttgttaaagg cgtcgaaacg caacgcccag tcgagaatat tacgcccaat
    16321 cagattcgga tcgatcttgc cttcacattc cagtaacgca tccgccagac acaggccatc
    16381 gaggtatcgt cggtgaattc ggcgcggtta aaataacagg ccgcgttatt ctcctttggt
    16441 ccaggcagaa aacggtcaat ccagccaaag tgtgctttaa cgcggctgcg tggccaaagc
    16501 tcggagggca tccccatcgc atcccctaac gcctgcccat aaagagcacc gagaatacgt
    16561 tctgttttca tttaacttcc ccttgtgtca acgcaatatc tcgatcatcg accttgatag
    16621 ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa cagcacggcg ataatggcaa
    16681 tcatcaccgc gccgaaagtc cacatcccgg accagttgaa agtcagtccg tttaccggtt
    16741 cctgataagc gaacatcttt tccatcatca caccgccaag acgatagccg agcaaactgc
    16801 cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc ggtacgcata tgcacggggg
    16861 cttttttatc gacatagatg taagcggtaa cgtagtaaaa atcgtaactt acgccgtgaa
    16921 gcaaaatacc gaggaacagt aacgcgtagg tgaaatattc atccgcacta ccgtaaataa
    16981 agaagccata gcagatcgca gcggtgacca gaccaagcaa taataccttt ttgataccaa
    17041 agcgtttagt gaaaaacggc aatgccagca taaagaagat ttcagagaac tggccgagcg
    17101 tcatccagcc ggtggcgttt ttcatgccaa cttcggtcag ataaccgttg gcaaagatgt
    17161 aatagaacgc cagtggcatc gcaaacagga atgaacagaa gaaaaagacg aggaagtttt
    17221 tatcgcgcag caggatcagc gcatccaggc cgagcatgac tttaatgtcc attttgccgg
    17281 tgctttttgg cggcgtgtcg ggcaggaaaa acgcaaacac accgagcaga gcagaacttc
    17341 cggcggtaat cagcagcggg atgttagtcg gtgagatatc ggcatacccc agcatttgcg
    17401 gcaagaaacc acatgccaga ccggaggcaa tccagccgat agtgcccatc acacgaatgc
    17461 gtgggaaatc acgctccaca tccggcacgt tggcaaaagc gatgctgtta gtcagcgcaa
    17521 tggtcggcat ataggttagc gagtaggcca gcagtaacgg aaagaacccg gcaaaagtgg
    17581 tctgttgcgc agcgaaatac atcagcacag caccagcgaa catcaatacc gccagcactt
    17641 tctgcgccga gaaaaagcgg tcagtgatgg agccaaccag aattggcgac aggatcgccg
    17701 caatggctgt gcaggcatac gaccagccaa tttctccggc actaaaaccg cttttactta
    17761 accacaacca caatggcaca aaccacgcgc cccagataaa ccattcaaca aacatcatga
    17821 acgacagctt tgctgttgtt ttcatctttt aattccttca tgacaggtaa tgtaaggtac
    17881 gcgatgacaa taccacataa taatgccttt tgaatacctt tgcggtgaaa acttgaccgc
    17941 gctaacattt tttctgatga atcgagccaa cagaaaacgc tgaaaaaaca tccaaaagat
    18001 ggaaaaactc gcccgtaacg actaggatta tgattgcccg caaaaggcgg gccaggacaa
    18061 tccatagccg atatccaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt
    18121 tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc
    18181 agctttatct ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc
    18241 cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat
    18301 atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg
    18361 ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta
    18421 actgtgtggc atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca
    18481 ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc
    18541 aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga
    18601 ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg
    18661 aacgcgcgca cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct
    18721 ttaagaaaga tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc
    18781 tggcggcaac cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct
    18841 ataaagcgat taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa
    18901 acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga
    18961 taaactccgg cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg
    19021 ttatcaacaa acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat
    19081 cgatggctga cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa
    19141 ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt
    19201 tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat
    19261 agcactgcca cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc
    19321 ttccctatcg ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt
    19381 ttttgtgatc attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa
    19441 agcaaacgac aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca
    19501 cagcgcggcg gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg
    19561 gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca
    19621 tttactcatg ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat
    19681 caccatccac tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag
    19741 gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat
    19801 atttcacggg tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg
    19861 gagctggggc aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg
    19921 tacaccaacc ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg
    19981 gtcgccatcg gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga
    20041 ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta
    20101 tcgactaagg atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg
    20161 gagctgaaaa atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg
    20221 accgatcccc gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa
    20281 gtgattgccg attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa
    20341 aaacatgaaa acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt
    20401 ctgttctgcc catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg
    20461 caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat
    20521 gacaccggca gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg
    20581 cgaacgcatt attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc
    20641 ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag
    20701 taaaattcat cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga
    20761 aacggttgcg gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg
    20821 ccagcgtgag caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc
    20881 cagcgccatt cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac
    20941 gcatcaaaag gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat
    21001 cgtggtgcag ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga
    21061 agcgcaggcg ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac
    21121 cgattaccag acccggacgg cttattggga attagtccgc gatcactttg caatattgaa
    21181 agtcggtccc gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga
    21241 gcaggaactt atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat
    21301 gctcgacgaa ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact
    21361 ggatattcgt tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa
    21421 aaatagcgtt gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat
    21481 tagtcagtat cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc
    21541 gcatcagctt attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc
    21601 ggaataagga cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg
    21661 tagcgaagtt ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac
    21721 ctggccacag gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca
    21781 gcacgccatt gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct
    21841 gttaaggcca acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt
    21901 atcgttggtt attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg
    21961 tttatttggc aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac
    22021 tcagttaaag gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta
    22081 acaataaaaa gtactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc
    22141 gacctctacg atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt
    22201 tgaattaatc cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat
    22261 atgtaccact gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc
    22321 ttttgtttct ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg
    22381 gtgacctatg ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct
    22441 gccggttgtc atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa
    22501 agcgggtctg catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct
    22561 ggattccatt ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt
    22621 ggtcgatgtc ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt
    22681 ggcgattccg attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt
    22741 ggtaaatgtt gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct
    22801 ggcaaccggt tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta
    22861 taagctctgc gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc
    22921 tattccgcac ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat
    22981 cgagaaaatc ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt
    23041 tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct
    23101 cgcgggttac gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct
    23161 gctgatgcca cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc
    23221 ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc
    23281 attactgctg gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat
    23341 tttaattgct gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat
    23401 cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat
    23461 ctcgggtgtc atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca
    23521 cacccaaccg gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga
    23581 tcagggcggt tctcccatta cctggttact gattcaggtt ttctccccgc aaaatattcc
    23641 cggtttcatt attatcggcg caatttatct gaccggtatt ttcatgacct ggcgtagagc
    23701 gcgtggtttt actaaacaag agaaagccgt tctcgcagaa taatttttac ctgaggggga
    23761 ggttatcccc cttaacaatc aggagttttt atgaaatcag tagtgaatga tactgatggt
    23821 atcgtgcgcg ttgcaaaaag cgtcattcct gaaattaaac atcaggatga ggtgcgggta
    23881 aaaattgcca gctcgggctt atgtggttcc gatttaccca ggatatttaa aaatggtgca
    23941 cattattatc caataacgtt aggccatgaa tttagcggct atattgatgc ggtgggatcc
    24001 ggtgttgatg atttacaccc tggcgatgcg gttgcctgtg tgccgttatt accctgtttt
    24061 acttgtccag agtgtttgaa agggttttat tcccagtgcg caaaatatga ttttattggc
    24121 tcgcggcgtg atggtggatt tgctgaatat attgtcgtta agcgaaaaaa tgtctttgct
    24181 ctacccacgg atatgcctat tgaggatggg gcttttattg agccgattac cgttggcctg
    24241 catgcttttc atttagcgca aggttgtgag aataaaaacg ttattattat tggtgccgga
    24301 accattggcc tgctggctat tcagtgcgct gtcgcgctgg gagcaaagag tgtgacggcg
    24361 atcgacatta gctcagaaaa actggcactg gcaaaatctt tcggtgcgat gcaaacattt
    24421 aacagtagcg aaatgagcgc gccgcaaatg cagagcgttt tacgcgaact gcgctttaat
    24481 cagcttatcc tcgagacggc tggcgtaccg caaactgtcg aactggcggt agagattgcc
    24541 ggtcctcatg cccaactggc gctggtgggc acgttgcatc aggatctgca tttaacatcg
    24601 gcaacgtttg gcaaaatatt gcgtaaagag ctgacggtta tcggcagttg gatgaactat
    24661 tccagccctt ggccggggca ggagtgggaa acggcgagcc ggttgctgac agaacgtaag
    24721 ttaagcctgg agccattaat cgctcaccgt ggaagctttg aaagcttcac ccaggcggtg
    24781 cgtgacatcg ctcgtaatgc catgccgggc aaagtgttgc tcattccatg aaaccgcggg
    24841 ccagcgtgat gctggcccgg tattgtgcaa aacagatcat tcaccaatgg tcccccttcg
    24901 tttacactag ccacaattga atatgggtaa atgacgatga attcattcga gcgaaggaat
    24961 aagatcatcc aattagtgaa tgaacaggga accgtgcttg ttcaggatct ggcgggagta
    25021 tttgctgcct cggaagcgac aatccgtgcc gatttgcgct ttctcgaaca aaaaggcgtg
    25081 gttacgcgct ttcatggcgg tgcggcgaaa ataatgtccg gtaatagtga aaccgagacc
    25141 caggaagtcg ggtttaaaga gcgatttcag ctcgccagcg cgccaaaaaa cagaatagcg
    25201 caggcggcag tcaaaatgat ccatgaaggg atgaccgtta ttctcgacag cggaagtacc
    25261 acaatgctta tcgccgaagg attaatgacc gctaaaaata tcacggtgat taccaacagt
    25321 ctcccggcag cgtttgccct ttccgaaaat aaagacatta ctctggtcgt ctgtggtggc
    25381 acggtccgcc ataaaacgcg ctcgatgcat ggttccattg ccgagcgttc attgcaggat
    25441 attaatgccg atttaatgtt tgtcggtgct gatggtattg atgcggttaa tggtattacg
    25501 acctttaatg aaggttattc gattagcggg gcgatggtca ccgccgcaaa taaagtgatt
    25561 gctgttctcg attcatcgaa atttaatcgt cgcggtttta atcaggtact gccaatcgaa
    25621 aaaattgaca ttattgttac cgatgatgct gtgtcagagg tggataaatt ggcattgcag
    25681 aagacacggg taaaattaat tacggtgtag tattgttctg tatgccggat aaggtgttta
    25741 caccgcatcc ggcattacgg attctccctg gttcaagaga gaatagtttg attaacgcaa
    25801 taatggacaa tctggtggta atcgacaacg caacgccgcc ggaagtattt caatatggaa
    25861 gttttgccca ctcaacggtt cgccatcaag attaaaagtg atttcgtgtg gggcttgtat
    25921 atcaaaccac gacgaagcgc cttcgataat attcgggtta tcttcgtcag attttaaggt
    25981 tgatacgaga gccggaagaa tttcatcgcc ggtaaaaatg cgcagttgca gcaagccatc
    26041 gttaattaac gcgttcgggc acaattgttg accgccaccg gcctgacgcc cgttaccaat
    26101 accaatgacc agggcgtcac cttgccagtg aaagttttca ccgcggattt cacaacggtc
    26161 cggttgcaga gtatccatgc gcattaagcc atgaatgatg taagagacgc cacccagcgc
    26221 ggcttttaat ttttccggcg tttctgtggt aatacgcgtc ccaaatccgc ctgtcgccat
    26281 attaataaaa caggtttgtt tgttgacctg cgccatatct atcgcaatgg cattaccggc
    26341 aattgccagt ttcagcgcct tatccagtgc ctcaggaatc cctacactgg tggcaaaatc
    26401 attggcggtt cctaatggca aaattcccag cgcgggtatg tcatccccct cacactgaat
    26461 caacgccgta gaaacttcat taatggtgcc atcgccacca ccggcaatca ccgttgcgac
    26521 gcccaacttc cgggcctcct ctacaaatcg tgcggcatca cctttctccc aggtgacccg
    26581 cacatggatc gtcattcctt cctcacgcaa cagcataatt gcttcgcgca agggtagatt
    26641 gtcagtactt ttaccattaa gaatcagtaa gctggcggga aattctgcca tgatcgtatg
    26701 tgcctttatg attggtctgt agatagtgta gagcagaaaa caaaaggtgg agtcagaaca
    26761 ggatgaaagt cggaggataa aaagtcagtt caagtatgga ggattcagtg gttaccacca
    26821 ggagctttca atggggacag cgaatactaa cggctgccac cgataaattt caaaaaagag
    26881 catataccta atattcaact aaacagtggc atcttcaata taatatatta aagcccccat
    26941 ggagttaccc tgaagggcct caatgtccgt aattcctact tatgtaggaa atgttgtaca
    27001 gagcatttat tataatccta ttcaattata ataatcatgt cattattata tttaaacact
    27061 agagagtgtc gttggtattt aatgggggaa ggtgagatga aaaagatagc tgctatatca
    27121 ttaattagta tttttcttat atctggttgt gctgtgcata atgatgaaac aagtatcggt
    27181 aaatttggtc ttgcatataa aagtaatatt cagcgtaaac tcgataacca atactacacc
    27241 gaagccgaag cttctttagc caggggcaga atatctggtg cagaaaatat agtaaaaaat
    27301 gatgcagccc atttctgtgt tactcagggc aaaaaaatgc agatagttga cctgaagaca
    27361 gaaggtgcag gattacatgg cgtcgctcgt ctgacattca aatgtggaga gtgagaatat
    27421 ttttttgtaa gcgtcaaaca tgcgcgttct ggctgtgcgt agccggaacc tgggcgagca
    27481 cgataccgtt acgtgaaagg cattgtgcta tgaaggggga ttctatcgat gtggtcaatg
    27541 gaagacggtt atcagggatc aatccttctt tacagcttat gtacaaaaaa taagcccgtg
    27601 taagggagat tacacaggct aaggaggtgg ttcctggtac agctagcatt ttatggatta
    27661 tgtttttcag cgaaacggat gataacctta ataaatgcag ctgtatgtga tcggtttcta
    27721 agaattttcc atccgggaaa aataatcgaa attaatcact taccgtgggg gttacgcgtg
    27781 gtttccccgg agaaattacg cattagcagc gcgtaattta gctcaagatc ctgcgggacc
    27841 gggagccaca cagtataacc atcgcctggt gcgaccggca tagcttcgcc tttggcgttt
    27901 tccatgtgct caagggtaaa gttaatgttg ccttgcggcg tcatcagctc aagactgtcg
    27961 ccaacggaga atttattttt caccgctacc gctgcgaggt cccccttgcg ctcaccggta
    28021 aactcaccaa caaactgctg gcggtcagaa actgaataac cgtattcgta gttctgataa
    28081 tcgtcgtgag tatgacgacg caggaaacct tcggtatagc cacgatgcgc cagaccttcc
    28141 agagtttcca gcaggctggt atcgaacggt tttcccgcag cggcgtcatc gatagctttg
    28201 cggtaaacct gtgcggtgcg tgcgcaatag tagaaagatt tggtacgacc ttcgattttc
    28261 agcgaatgca cgcccatttt ggtcaggcgt tctacatggg cgatggcgcg cagatctttc
    28321 gagttcatga tgtaagtgcc gtgctcatct tcaaacgcgg tcatatactc gcccggacgc
    28381 tgggcttctt cgatcataaa cactttgtcg gttggcgcgc cgatacccag cgtcggctca
    28441 acattttgca ccggaatcgg ctcgtacttg tgtacgatgt tgccaacgtc atcttctttc
    28501 ccttcctgga cgttgtactc ccagcggcag gcgttggtgc aggtgccctg gttcgggtcg
    28561 cgcttgttga tatagccaga gagcaggcag cgaccggagt aggccatgca cagtgcgccg
    28621 tgaacgaaga tctcgatctc catatccggc acctgattat ggatctcttc aatctcttcc
    28681 agcgacagct cgcgagagag gatcacgcgg gtcagtccca tttgctgcca gaatttcacc
    28741 gtcgcccagt tcacggcgtt agcctgcacc gagaggtgga tcggcatttc agggaagtgc
    28801 tcacgcacca gcataatcag ccctggatcg gacataatca gcgcatccgg ccccatttcc
    28861 accaccggtt tcaggtcacg gataaaggtt ttcagcttgg cgttgtgcgg tgcaatgttg
    28921 accacgacat aaaacttttt ccccagcgcg tgggcttcat tgatgccgag ctgaagattt
    28981 tcgtggttga attcgttgtt gcgcacacgt agggagtaac gcggctggcc cgcataaaca
    29041 gcatctgcgc cataagcgaa agcgtaacgc atatttttca gcgttcccgc cggggaaagg
    29101 agttccggtt taaacataat tctctcgttc tgatgacagg tcagatccgc ttcacctgat
    29161 gaagcggtta ggggaggccc ccactttaag ggcgggcatt gtagcgctgt aggggggatc
    29221 tggtaaagcg ccggacgcca acaacccacg cccggcatcg taattacgta ttgtctttca
    29281 ccgtctggct tgtgccattg gctttaacgg aggcaattcc ggtttcacga gattgcgcgg
    29341 tggcgtacat ctggctggag ccgataattt gatgattagc ggctttcaga ttgaaataga
    29401 atttgccgtt gcttgccgtt ttttctcata gcgctcttct tgcgggctgt tgctacgcac
    29461 cgacgcgatg cccttttccg cagaggcttt tgaggtatac agctcgctgg tgaggatagt
    29521 ctcaccattg cccgctttta gcacaaaccg gaactgatta tcactgctct tactgagttc
    29581 aaaccaacca gccataatga ctccttgatt gaaattgctt atcgaaaaca gcatct
//
LOCUS       DAJVRL010000047        29236 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVRL010000047 DAJVRL010000000
VERSION     DAJVRL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.047"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..218
                     /locus_tag="OQN02_002054"
     CDS             <1..218
                     /locus_tag="OQN02_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1642844.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OQN02_002055"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OQN02_002055"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1642845.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OQN02_002056"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OQN02_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1642846.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="OQN02_002057"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="OQN02_002057"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1642847.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="OQN02_002058"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="OQN02_002058"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1642848.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="OQN02_002059"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="OQN02_002059"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1642849.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="OQN02_002060"
     CDS             7229..8179
                     /locus_tag="OQN02_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1642850.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="OQN02_002061"
     CDS             complement(8220..8690)
                     /locus_tag="OQN02_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1642851.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="OQN02_002062"
     CDS             complement(8745..9947)
                     /locus_tag="OQN02_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS1642852.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="OQN02_002063"
     CDS             complement(10034..10576)
                     /locus_tag="OQN02_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642853.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="OQN02_002064"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="OQN02_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1642854.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="OQN02_002065"
     CDS             complement(11319..11921)
                     /locus_tag="OQN02_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1642855.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="OQN02_002066"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="OQN02_002066"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1642856.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="OQN02_002067"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="OQN02_002067"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1642857.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="OQN02_002068"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="OQN02_002068"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS1642858.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="OQN02_002069"
     CDS             16319..17209
                     /locus_tag="OQN02_002069"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1642859.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="OQN02_002070"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="OQN02_002070"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1642860.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="OQN02_002071"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="OQN02_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1642861.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="OQN02_002072"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="OQN02_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1642862.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OQN02_002073"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OQN02_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1642863.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OQN02_002074"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OQN02_002074"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1642864.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OQN02_002075"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OQN02_002075"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1642865.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OQN02_002076"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OQN02_002076"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1642866.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OQN02_002077"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OQN02_002077"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1642867.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OQN02_002078"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OQN02_002078"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1642868.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OQN02_002079"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OQN02_002079"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1642869.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OQN02_002080"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OQN02_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1642870.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="OQN02_002081"
     CDS             complement(27384..27845)
                     /locus_tag="OQN02_002081"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1642871.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="OQN02_002082"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="OQN02_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS1642872.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="OQN02_002083"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="OQN02_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7086 a         7856 c         7300 g         6994 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctctcgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DAJVRL010000048        29080 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVRL010000048 DAJVRL010000000
VERSION     DAJVRL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.048"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(173..1069)
                     /gene="yhaJ"
                     /locus_tag="OQN02_002084"
     CDS             complement(173..1069)
                     /gene="yhaJ"
                     /locus_tag="OQN02_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCS1642873.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1174..1875
                     /locus_tag="OQN02_002085"
     CDS             1174..1875
                     /locus_tag="OQN02_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1642874.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1898..2062
                     /locus_tag="OQN02_002086"
     CDS             1898..2062
                     /locus_tag="OQN02_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642875.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2274..3584)
                     /locus_tag="OQN02_002087"
     CDS             complement(2274..3584)
                     /locus_tag="OQN02_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1642876.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3612..4943)
                     /locus_tag="OQN02_002088"
     CDS             complement(3612..4943)
                     /locus_tag="OQN02_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1642877.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="OQN02_002089"
     CDS             complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="OQN02_002089"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1642878.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6654..7043)
                     /locus_tag="OQN02_002090"
     CDS             complement(6654..7043)
                     /locus_tag="OQN02_002090"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1642879.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="OQN02_002091"
     CDS             complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="OQN02_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1642880.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="OQN02_002092"
     CDS             complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="OQN02_002092"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1642881.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="OQN02_002093"
     CDS             complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="OQN02_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1642882.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="OQN02_002094"
     CDS             complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="OQN02_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1642883.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="OQN02_002095"
     CDS             complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="OQN02_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1642884.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14188..14532
                     /gene="tdcR"
                     /locus_tag="OQN02_002096"
     CDS             14188..14532
                     /gene="tdcR"
                     /locus_tag="OQN02_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1642885.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14788..15327
                     /locus_tag="OQN02_002097"
     CDS             14788..15327
                     /locus_tag="OQN02_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642886.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15349..15696
                     /locus_tag="OQN02_002098"
     CDS             15349..15696
                     /locus_tag="OQN02_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642887.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15865..16515
                     /locus_tag="OQN02_002099"
     CDS             15865..16515
                     /locus_tag="OQN02_002099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642888.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(17023..17399)
                     /gene="rnpB"
                     /locus_tag="OQN02_002100"
     ncRNA           complement(17023..17399)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQN02_002100"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17432..18577)
                     /gene="garK"
                     /locus_tag="OQN02_002101"
                     /pseudo
     CDS             complement(17432..18577)
                     /gene="garK"
                     /locus_tag="OQN02_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(18674..19564)
                     /gene="garR"
                     /locus_tag="OQN02_002102"
     CDS             complement(18674..19564)
                     /gene="garR"
                     /locus_tag="OQN02_002102"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1642889.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(19594..20364)
                     /gene="garL"
                     /locus_tag="OQN02_002103"
                     /pseudo
     CDS             complement(19594..20364)
                     /gene="garL"
                     /locus_tag="OQN02_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(20380..21714)
                     /gene="garP"
                     /locus_tag="OQN02_002104"
     CDS             complement(20380..21714)
                     /gene="garP"
                     /locus_tag="OQN02_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1642890.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            22089..23660
                     /gene="garD"
                     /locus_tag="OQN02_002105"
     CDS             22089..23660
                     /gene="garD"
                     /locus_tag="OQN02_002105"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCS1642891.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(23712..24536)
                     /gene="agaR"
                     /locus_tag="OQN02_002106"
     CDS             complement(23712..24536)
                     /gene="agaR"
                     /locus_tag="OQN02_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCS1642892.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            24785..26065
                     /gene="kbaZ"
                     /locus_tag="OQN02_002107"
     CDS             24785..26065
                     /gene="kbaZ"
                     /locus_tag="OQN02_002107"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCS1642893.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            26088..26561
                     /gene="agaV"
                     /locus_tag="OQN02_002108"
     CDS             26088..26561
                     /gene="agaV"
                     /locus_tag="OQN02_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCS1642894.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            26572..27351
                     /locus_tag="OQN02_002109"
     CDS             26572..27351
                     /locus_tag="OQN02_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCS1642895.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            27341..28150
                     /locus_tag="OQN02_002110"
     CDS             27341..28150
                     /locus_tag="OQN02_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCS1642896.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            28237..28671
                     /locus_tag="OQN02_002111"
     CDS             28237..28671
                     /locus_tag="OQN02_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1642897.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            28668..>29080
                     /locus_tag="OQN02_002112"
     CDS             28668..>29080
                     /locus_tag="OQN02_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1642898.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         7565 a         7592 c         6864 g         7059 t
ORIGIN      
        1 ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc agcctacaaa
       61 ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag cgcatcaggc
      121 agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata gattattttc
      181 cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc atactgtcac
      241 gacgccaagc cataataata tcgatctcgc tggtcgattc cgggctgacg acacgcaacc
      301 gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg ccaagccccg
      361 ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca
      421 gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca attccacgat
      481 atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg tgatcaggcg
      541 cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc gacgaggaac
      601 gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc cgttcccacg
      661 cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc gccagtttgt
      721 cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg gtgagatgcg
      781 tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg gcttccagca
      841 aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg cgatggcccg
      901 agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatggtg tagctaagtg
      961 cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg cgatcgatcg
     1021 catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt tcgttctcat
     1081 tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt ccagcccctt
     1141 accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac tgccaggcag
     1201 tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt tggacactac
     1261 ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga agtgctggcc
     1321 ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa tgtgattctg
     1381 gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag cgccggagag
     1441 gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct cagcaaagac
     1501 aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga gaatccgctg
     1561 attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc agagggtgcg
     1621 atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct cgacaaaggc
     1681 gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat tcacgggaaa
     1741 tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga tggggcgttt
     1801 attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc tttgctgata
     1861 gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat cgaccaaaaa
     1921 gcgccagcca gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca aaaattttgg
     1981 ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa cgcgtttagc
     2041 cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg ccttatccgg
     2101 cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata agatgcgcca
     2161 gcatcgtatc cggcatcagg tacaaaacac aacaacatca acaagttgag caccccgtag
     2221 gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg cgtttatctg
     2281 gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga atggcttgct
     2341 aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc ttcattgccg
     2401 gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga agcactggtc
     2461 gaaaccttca tcgcgcagct gttcgacgca ccatcgcaaa tcatgccgct gacatcgccg
     2521 atcatactgc tgatcgccat cgagatagtt tcataacgcc catccaccag ccatgccatc
     2581 ccggcggcgg cccccattgc tgcggtcgtt gcggcacaaa gtgcagacaa acgcggtaac
     2641 tggttatgga tgtaaattgc gctcaaatga gaaagcatca gcgcacgcgc cagtcgttca
     2701 tcatccgctc cgaagtgttc tgctacaacc accacaggca ttattgcagt gatcccctgg
     2761 ttacccgagc cggagttact catagccgga agcgtagcgc cgcccatacg cgcatcggat
     2821 gccgcgctgg tacgaatcac aatggatgaa gagagatctt tcgccagcaa accgcgctcg
     2881 cactgttttt ccagcgtcgc gccaatatgc agcccccact taccgctcaa accttcctgc
     2941 gataacgcac aatttagctt cgcggaatcg agaataaagc ggatcgccgc aaatgggact
     3001 tcattgacga acttcaggat ctcagccagc gtcgttctgg aaagcaccgt cagcggcgac
     3061 tcttgctcgc cctctgccac acacgcctgg tgggtaaaca ccacaccatt gtgcgtctca
     3121 atatgcacaa tgttggtatg cccgccgacg atggtgacac acgcccactt ctcaccgttc
     3181 cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt cctggatctt aacagagact
     3241 ttccccgccg ccagcagtgc tttggcatcg gcaattgcct gcgcagttgc gtctttcagc
     3301 acttccagcc cggcgttggc atttccacct aacgccccca gcgccgccgc aatcggcagc
     3361 cccaccattc ccgtgccggg aacggtgacg cccagaccgt tcttcatcag atttggcgaa
     3421 acccaggctt ctacacgttc aaccggacct tccagttctg ccgcagcaac cgccgccgcc
     3481 agcgccagtg aaatcggttc agtacatccc agcgccggtt ttacttcctc ctgaacggcg
     3541 aggatgtaac gctgccataa cggatttaaa gtcgaatcaa acatattaaa aaccttaaaa
     3601 tttcaggtaa atcaagaaaa tgccaggaac agagaaacac aaagcaacaa accagtgacg
     3661 ataatcaggt acagagacat ccctttgtat ttgtgcaatg ccggtacttt gtaaaccagc
     3721 cacgccggga tcaggcaccc taccatgccg aaaatcgggc tacagataga ggtgaagctc
     3781 aacaccggtg cgttcagtac gatggcgctc caggccagca aaatggcgaa aatcatgatg
     3841 ccgcgctgaa caagattttc gttaatcttc tcggcaggca tcttgcgacg caggatgttc
     3901 attacgatcc cttgcgttgc ttcgcgaaag cctaaataga cgccaaagaa tgcagtcatg
     3961 acagcaaaga tattgagaat gacgctgacc actttcaccc acgctgcgcc gtcaccgcta
     4021 ataaactgtg cggcaatcgc cagcgcggaa atattctgct cgtaggcttt taccgcttcg
     4081 tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa agacggtgac aaacaaaatg
     4141 ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg ccacttcaat tgatttttcc
     4201 cgcgagcgat aagagatcac catcgggctt aacgtctgga taaacagaat cgacgtcagg
     4261 gtaaacggca gcgtaataat ggcgtttttc accagcagcc ccagcggcgg tagcgaaccg
     4321 acgttataca gatgccacat tcccaccatc gacacgccca gcgcagcgac taccagcagc
     4381 ttggtcagca ccatgtcggt cgaaattttg aataacaatt tctcgccgcg tgaagagatc
     4441 gccaccagaa tgcaaatcag taccagacca taaaagggac tgtctgacag caacccttcc
     4501 gtcacgccga aggtatgcag ataggaagca ctatcgttgg tgatggcggt ggaatagacg
     4561 aacatccaaa tcaccagcat tacgaaatag agcgcaccta acaggatgcc ccagttttta
     4621 cctaaataac cgctaatgac actcgggtaa tctttacatt ctggtgattc tgccagcgta
     4681 ttaataaaca accgctgaaa cagatacatt gccgggtaac caatcaccga tgagagcaaa
     4741 aatacccaca atcccatcaa accgacctgc accgggagaa aaacaatccc cgcgccaatc
     4801 gccatcccga tactcataat cacccagccg gtgtcagtac tgtcgaattt gatcgcttct
     4861 cgccactcgc tctcactcat tccagcacga cccgccgggg tcgaagcgtc tgcaatgacg
     4921 cctttattcg atgcaatttc cataatttct cgctcaatat tttgtagggc ttattatttt
     4981 tttccgagcc gcatcaaggc gatatgcggt ctacgttttt gcaggcgagt tattagaata
     5041 aagaaatgat acgcgcgagg tcagagaaaa tcttcacaat caagacttta aaataacgga
     5101 gatggataag aattttctac taaattaatc gcaagagaat attcattctc tatttgcggc
     5161 agatcacaaa aaaaaggtgc acatttgtgc acccaaggat gaaagctgac agcaatgtca
     5221 gccgcagacc actttaatgg ccagtcctcc gcgtgatgtt tcgcggtatt tatcgttcat
     5281 atctttgccg gtttcataca tcgtctcgat cactttatcg agtgaaacac gcggtgccga
     5341 ggtgcggcgc atcgccatcc gcgcggcgtt tactgctttc acggcattaa tggcattacg
     5401 ttcaatgcac gggatttgta cctgtccggc aaccggatcg caggtcagcc caaggttatg
     5461 ctccatcgcg atttccgccg cattgcatac ctgcgccgga ctgccgccca gtagttcagt
     5521 taaccctgcc gctgccattg aacaggccac gccaatctcc ccctgacagc cgacctccgc
     5581 gccagagatg gaggcgttca ttttatacag cgcgccaata gcccccgctg ccagaaaata
     5641 gcgggcaatt gaccgctcgt ttaccggacg acggaactta tcgtaataag ccagcactgc
     5701 cggaataatg ccgcacgcac cgttagtcgg tgccgttacc acgcgcccgc cagctgcgtt
     5761 ttcttcacta accgccagcg cgtacatgtt gatccagtcg atgacattca tcggatcgtt
     5821 agagatgtta tcgctagaaa ccagctgacg acgcagcgct acggcacgac acggcacatt
     5881 gagcggacca ggcagcacgc cttcagtgtt catcccgcgt tcaataccgt catgcatcac
     5941 ttgccagata cgggcaaacc ctgcgtcaat ttccgctttg ctgcgcagcg ccagctcgtt
     6001 gtgcatcatc aggccagata tagacaggcc gttgtaatca cacattttca gcagttcacc
     6061 tgctgagtgg aaatcgtaag gtacggacgt ttcgacatcg tgcgacaggc cgaagtgttc
     6121 ttcttcgaca ataaacccgc cgccgacaga gtaataggtt ttacttaata gcgcttcctg
     6181 tcccttccag gcagtgatcc gcattccgtt ctcatggcga ggcaacattt cgggatggaa
     6241 gataatgttc tttgctacag gaaaatcaac aatatgcgca ccagatgcca ctggcagccg
     6301 cccgctgcgc gttactaact ctataaatgc agggatctca tcaatgacaa catcctgcgg
     6361 actgtttcct gccagtccca tgataatggc gacatccgtg gcatggcctt tgcccgtcag
     6421 tgacaacgac ccgtacagat cgaccacaat atggctcgtc gcggttaata agccgctact
     6481 ttccagccga tcaataaaac tttttccggc attcattggc cccacggtat gcgaactgga
     6541 gggaccaatc ccaattttga aaatatcgaa tgcactaatc atatccacac cctcggattg
     6601 ccgttcagtg aagtggagcg gaacgacctt gcgaccgtcc cgctcacgag gctttacgca
     6661 ctacgtactg cgatagcttc aatttccagc ttcacatctt tcggcaaacg cgcaacctgc
     6721 acacagctcc gggtcgggta ggtcgcctga tgctcatcga agaactgctt atagacttca
     6781 ttgatggtgg caaaatcatt cagatcggtg atgaacacgg tcatcttgat gatatcgccc
     6841 acgctcagcc cggcagcaac cacgatcgct ttgacgtttt cgaggcttaa acgtgcctga
     6901 tcttgcacat cagccgggat ctcaccggtc tgcgggcaaa ccggaatttg cccggaggtg
     6961 aagaccatgc tgcctaaatc aacgccctga acataagggc cgattgcgcc tggggcacgt
     7021 tgcgtttcga taatcttttt catacatcct ccggcgtcag agcgcctggg taaaagtacg
     7081 tgaaataaca tcctgttgct gttcacgggt cagtgcgttg aagcgcacgg cgtagccaga
     7141 gacacggatt gtcaggttag gatatttttc cgggtgctcg atggcatcca gcagcatttc
     7201 ccgattcatt acgttgacgt tgaggtgttg accgccttcg acatccgctt cgtggtggaa
     7261 atacccatcc agcaggccaa caaggttggt tttacgtact ggatcttctt tgcccagcgc
     7321 cgcaggaacg attgagaagg tgtacgagat cccatctttg gcgtaggtga acggcagttt
     7381 cgccaccgac gtcaatgagg ccacggcacc tttgcggtca cgaccatgca tcgggttagc
     7441 gcccggcgcg aacggtgttc cggcgcgacg accgtccggc gtattaccgg ttttctggcc
     7501 gtacaccacg ttagaagtga tagtcagaat cgactgggta gggacggcgt tgcgataggt
     7561 tggcagcgct ttaattttct tcataaagcg ttcaaccagg tcgcaggcaa tgctgtctac
     7621 gcgctcgtcg ttgttgccgt actgcggata ttcaccgtcg atttcaaagt ccaccgccag
     7681 gccgttttcg tcacggattg gtttcacgcg ggcatatttg atggcagaca gggagtccgt
     7741 cgccaccgac aggcccgcga tgccgcatgc catagtgcga tagacatcac gatcgtgcag
     7801 cgccatcagc gaagcttcgt agctgtactt gtcgtgcatg tagtgaatga tattcagcgc
     7861 gctgatgtac tgcaccgcca gccagtccat gaagtgatcg aggctgtcca tcactttgtc
     7921 gtaatccagc acgtcgtcca tcagcggtgc tgttttcggc ccgacctgaa tcttcagctt
     7981 ctcgtccacc ccgccgttaa ttgcgtagag cagcgttttc gccaggttag cgcgtgcacc
     8041 aaagaactgc atttgcttac caatcaccat tgggctgacg cagcaggcaa tcgcgtaatc
     8101 gtcgctgttg aagtcagtac gcatcagatc gtcattttca tactgcaagg aagaggtgac
     8161 gatcgacacc tgcgcggcat attttttgaa ggcaatcggt aattcttccg accaaagaat
     8221 ggtcaggtta ggttccggtg ccggccccat agtgtgcagg gtgtgcaaat agcggaagga
     8281 gtttttggtc accagcgtac gaccgtccag ccccatcccg ccgatcactt ccgtcgccca
     8341 gattgggtcg ccggagaaca gcgaatcaaa ttccggtgta cgcaggaagc gtaccatacg
     8401 gatcttcatg atgaagtgat cgatcagttc ctgtgcctgc tgctcattga ggacgccagc
     8461 tttaaagtcg cgctcaatgt agatatccag gaacgatgcc gtgcgcccca gcgacatcgc
     8521 gccgccattt tgcgatttca ctgccgccag ataagcgaag tagagccact gcaccgcttc
     8581 ctgtgcattc tgcgccgggc gagagatatc aaagccatat ttcgccgcca tttcctgaat
     8641 ctgcaacagc gcatgacgat gctctgccag ctcctcacgc agacggatgg tggcttccag
     8701 atcctcgcct ttttccagac gagactggag atcggcaaat tgcagttcgc gttcacgcac
     8761 cagataactg atgccataca gcgctacgcg gcgatagtca ccgataatgc gcccacgacc
     8821 atagccatct ggtaaaccgg tcagcacgcc agatttacgg cagcgcagca tatccggtga
     8881 gtaaacatca aatacgccct ggttatgggt tttacgcaga tcggtaaaca gatattcaaa
     8941 ttcactgtcc atttcacggc cataggcgtg gaatgaactt ttaatcatat taatgccacc
     9001 gaacgggtgt agcgcacgtt tcaacggcgc atccgtttgc aggccaacaa ttttttccag
     9061 cggctggtta atatatcccg catcatgagc ggtaattgtg gtggcaatat tggtatcgaa
     9121 atcaaccggc gcgtgggttg cgttttcgat acggatgcct tccattacct tttcccacaa
     9181 ttccgtggtg gcaggcgtcg cttcggcgag aaaagattca tcgccttcat acggtgtata
     9241 gttatgttga ataaaatcgc ggacattaat ttcgcttttc cagtccgtac ctttaaagcc
     9301 aagccatgcg tcagcgtaca gcttatcgct ggtatcaata tctaccttca tgaaaaataa
     9361 tctctctaca atacttcaac taaattatgc aaattctgcg ggcgcgttaa ctttacctaa
     9421 atgaatggca tccaaagcaa tcattttttc ttcgttagtc ggaataacga cgcaaatgac
     9481 atgcgcattt tcactggaaa caattcgctc gccaaaagag ttagatcgat tattcatttc
     9541 tgtatcaatc tctacgccta atacagccaa atgttccatg accagacgac gaattaaact
     9601 tgaattctct cctattccgc cggtgaatat aattccatcc agccgacgta atgaagctgc
     9661 atgtccggca atatgacggg caattcggtg aacaaaggtt ttaattgcca gttgcgcgcg
     9721 ttcgtgacct tcatgccagg ctttttccag aacacgtaaa tccgaagaaa gaccggaaat
     9781 acctaataat cccgactctt tattcactac gcgttccagg tcacccaggc tctggttggt
     9841 ttggctggcg acccaggaca tcgcaccaaa gtcgacatcg ccactgcggg tacccatcat
     9901 caagccttcc agcggcgtca ttcccattga ggtgtcaaca ctctgaccgt tgcgaactgc
     9961 gcagattgac gcgccattgc caagatgcgc cacaaccagg ccggagtcat cttccgccag
    10021 attcagcagc gaatgtgcgc gctgggaaac atagcggtgc gacgtgccgt ggaaaccata
    10081 acggcgtaca cctaactctt cataatattt ccacggcagg ccgtataaat aagcttccgg
    10141 agccatcgtc tggtggaaac tggtatcaaa taccgccacc tgagttacgc ccggaaataa
    10201 ttgctgcgcc gattcaatac cacttaaatt tgcgtaatta tgcaggggtg ccagtggaga
    10261 aacgcgacgg atattatcaa tgacttcatc ggtaataatg gcggactcgg taaaaatact
    10321 gccgccgtga gcgatgcggt ggccaattaa ggccacgctg tcatttaaat tccgtttttc
    10381 cagttcaaat gcaattgcct tcaatgcacc ttcgtagctg tggtgagcca gcggtgctgg
    10441 ctctccccca tttacggata agaatgcatt ttccgagtta ataccgtcgg caatacctga
    10501 cattaatact tcacaatcgc tggcatcgag tacggaaaac ttaatcgaag acgaaccaca
    10561 gttaataacc aaaacaaccg gaaattcatt catctctttt ctcatcctga gttacggatt
    10621 aaaacagttt gtatacgatg ttcaggatgg tcagcagacc aatcacggta acaaacacgt
    10681 tatccagacg accacggtat ttcgccagag acggcgcttt acggatagca tacatcggca
    10741 acaggcacag cagggatgcg ataatcggtg cgcccatggc ttcaatcagg tcgaggatgt
    10801 tcgggttggc gtaggcaaca acccaggtgg agcccatgat gaagatcatg ctgagagtat
    10861 tcagtttacc cagcgacact ttggttttgt cacctttata accgaacttc agaatcagac
    10921 cattcaagcc ttccagcgtc cccagatagt gaccgaagaa agatttgaag atagccacga
    10981 gtgcgatgat ggaagccgca tattccagtg taatcgcgaa cgttgttttc gtaccggtca
    11041 tggacgcaaa gtggttagcc agataagaaa gcactggaat attctgcgct ttggcttccg
    11101 ccatgttggc cggagacaga gtaaacaggc agctaaaggc aaagaacatc accactgcaa
    11161 ccatcagcat gctggcacga gaaatgattt gggaacattt acgttcggtg aagtcgcgac
    11221 cgaagtcttt ctcatactct tcacgtttag aaaccacgaa ggaagagacg attggcgaga
    11281 agttaaagga gaaaaccatg atggaaatcc ccagccagac agtgatcagg ataccgtcat
    11341 gaccggttaa cgacagcgaa ccgaggtcaa cctggtcgat aactgcagag ttccagtaag
    11401 ggatcagcga cagagaaatc agcaccaggc tggcgataaa cggccatacc aggtagctca
    11461 tcactttaac catcagatcc ttaccaaacc agatgacgaa agccatcagc agcaacagga
    11521 acagcgccac aaagccgcga ttcagcggtg caaatccgag ctggttttcc cagaacgtca
    11581 taaaggtatt ggtaatagta acgccataaa tccacagcag tgggcaaatc gcgaagaagt
    11641 acaggaacgt gataaccacg ccgccagttt taccaaaatg ctcttccacc gtttccgtaa
    11701 tgttgccgga agggttagag ccagaaagac acagacgcgc cagcgcccgg tggcaataaa
    11761 atgcgatggg gtatgccaat accaacatca gaagaatcgg gatcagtccg ccaaaacctg
    11821 cgcggatagg gaagaacagc accccggcgc cgattgccgt accaaacaag cctaacgtcc
    11881 atgtggtatc tgatttacgc caggacgatt gttttgtctg gctggataca atgctatctg
    11941 aagtactcat atcctatcct caacgaatta attaagcgtc aacgaaaccg gtgatttgag
    12001 agacgcgaga aagatcgata ttgccgccgg aaataatact gacggttttt ctgttttgaa
    12061 tatattggtc taatttaccg cttaataatg cagcacatgc cagagcgcct gcgccttcgg
    12121 tgacgacttt attgcgctga attaaggcaa tcatactgtt tctgatttcg tcttcgctga
    12181 ccagcacgat gtcatcgact aattcacgaa cgatttcgta agttaaatta cccgggcggg
    12241 agacatcaca accatccgcc agggtgccgg tagttcggtg cgtggttatt tctccggagt
    12301 ggaaagaagc cgccatgccg tgaacgtttt ctgactgtac gccaataaca cgaatggtcg
    12361 ggttaataga tttaattgcc accgcaatac cagcaattaa accaccacca ccaattggca
    12421 caatcacgtt atcgacatca tagagatctt ccataatttc cagaccaatc gttccctggc
    12481 cagcaatcac tttcggatca tcgtaaggtg ggataaaaat acgaccttcc atttcgacaa
    12541 tttcgctcac tttagcgata gtgtcgttga agttatcacc atgcagaacg acttctgcgg
    12601 agtagtcgca cgttgccgct actttggatt ttggcgcacc ttttggcatc accactttac
    12661 cgtcgatacc cagcatcgcg caggagagcg aaaccccttg cgcatggttg cccgcagaac
    12721 aggccaccac gcctttgcgt ttttccgcat cggtcagtga acttaattta ttaaatgcgc
    12781 cacgaatttt aaatgaaccc gtacgctgca tattttcaaa cttcaggaat atttcacctt
    12841 tgcaacgttc actaaaatag ttggagcgag gcatgcctgt tttataaatt cgcccagcca
    12901 gtcgttgttt cgcttcaata atatcatcaa tagcaaccgg cagatcgtat gtaatatgca
    12961 ttataaaacc tcttcgcctg aattaaatat gtaggtaacc gtaaccgaca cctgcaaaac
    13021 agacaggtgg attatttatg tttaaataaa aacaaacaac taaccaactt ctattaattg
    13081 ccttcgtcta cacccattat aagatgaata ctctttggct aattccacca aaaccgatgc
    13141 tgctttttta atacgataat ttttcgacca taccgcggca tattgtgcca caggtaatgt
    13201 ttcttcaacc ggaatagtaa taaattgatt agaaccaaaa ggtgacgtca tatcacaagg
    13261 aattacagtt aagaaatcag cattgagaac aagattataa attgtcacga ctgagtcggt
    13321 tttaacgatg ttttcaatac tgatgccatt tctttgtaac gtagtaagca gttcgctgta
    13381 gtaccccata ttagtttgtg gcaacaccca ctgttcgttc ttcaacgact ccagcgtggt
    13441 ggtgccggtg catgttcggg acttactggc taccagcaca aactcggact cgaacagcgg
    13501 ctcaacatgt aaatcctgaa gcttcatttc tgcacttaac gtaccaatcg caaaatccag
    13561 tcgaccgtcg cggattgccg gtaagaacga agacagttgc gcttcataca tagaaacctg
    13621 cgctttcggg aacacctctt tgaacttgtt gatcatccct gacataaaag taaaaccaat
    13681 caatgaagga aaaccaaatg agacttccac caccgcttca gaagacatac cgcttatctc
    13741 attaaccata tttttcattt cacgggtaat ggattcggaa cgggagagta acaattgacc
    13801 ggcaggtgtt aatgttacac cggtattttt ccgcaccact aattccacac caaaataatc
    13861 ttcaatatcg ttaatgattt tactgacggc cggttgagtt aaccctaatt cttttgcagc
    13921 cgagccgata gagccacttc taatgacttc ctgaaagact accaggtgct gcgttttcgg
    13981 aagaagaata gtgctcataa cgacctacgt taattacctc attgacggca tgaagtgtat
    14041 caaaatgaaa taaacaggat atgtgcgacc actcacaaat taactttcat tactttccaa
    14101 ggtatcgtta ttacaattaa atatctttaa atatcaacaa gttaaagcat aaaaatcagc
    14161 ataaaccctg atttttatca aaaaaaaatg acggggataa ccatatttta tggcgataac
    14221 atcattcgtt atgtggttaa tacaaaaaag gggctgagac catatttcaa gcaattaccg
    14281 gataattacc aggcaaaatt tgagttaaat ttaatgagca agttctcaaa ttttattata
    14341 aataaaccat tttcagcgat aaatactgcg gcacgtcaca ttttttcacg ctatttgttg
    14401 gagaacaaac atttatttta tcaatatttt aaaatttcga atacaggtat tgatcatctc
    14461 gaacaattga ttaacgtcaa ctttttctct tctgacagga cgtcattttg tgaatgcaat
    14521 cgttttccat aaattcttct cccctcatag gcgacgaata gcattttgtg ttgaggatca
    14581 caaaacgaat aattgctggt cgccgcgata aggtcagacg aagtcaacaa gggggatttt
    14641 cactgtgctt tacatcagcg taggacacag aatgttgctt cgtttactgt aacgccgggt
    14701 aaatgagcgt tttttgatag tgcgtaagtg tcgtgcgagg aaaataagca tctattattg
    14761 tttgtcatga cgacaaggca ggcatcaatg aaaggcttcc caattgcaca tatttttcac
    14821 ccttcaatcc cgccaatgca cgcagtggtt aacaatcaca atagaaatat tgattattgg
    14881 acggtaaaaa gaaagtttgc agaaattgtc tccaccaatg acgttaataa aatttacagt
    14941 ataagtaatg aactccggag agtattatct gcaattactg cattgaattt ctatcaaggc
    15001 gatgttcctt ctgtcatgat ccgaatccaa ccggaaaata tgagtccatt catcatagat
    15061 atttctacag gagaacatga tgattatatc atacaaacat tagatgtagg cacttttgca
    15121 ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa aagagctgga atgtattaag
    15181 gagacgattt ctaagtattg cgcaaagttc acccaaaaag aagccatctt aacaccccct
    15241 aggcacttta ataaaacctg cattacttca gattgttggc aaattctctt tttttcaccc
    15301 gatcatttta ataatgattt ttattgatta tttataaagg ataaaataat gttccctgtt
    15361 tcatcaattg gtaatgatat tagtagtgat ttagtccgtc gaaaaatgaa tgaccttcct
    15421 gaaagcccca taggaaataa cctcgaagca ctagcaccag gtatagaaaa actaaaacag
    15481 acctctattc aaatggtcac tttacttaac gcgttacaac caagtggaaa atgcattatc
    15541 actggtgatt ttcaaaaaga attagcgtac ttacaaaatg taattcttta taatgactcg
    15601 tctcttcgtc tggatttttt aggttataac acccaaatta ttcaacgatc ggacaatacc
    15661 tgcgaactta ccattaatga accgttaaaa aactaggaaa tatctacagg caatatcaat
    15721 gttaattgcc cattaaaaga tatttacaat gaaatcagga ggttgaacgt aatctttagt
    15781 tgtgggactg gagatatagt tgatctatcc tctctggact tacgtaatgt cgatttagat
    15841 tattatgatt tcacagataa acatatggct aatactattc taaatcattt taaattggat
    15901 tcaacaaatt ttactaatgc caacatgttt caggttaatt ttgttagttc aacacaaaac
    15961 gccacaatct cctgggatta tttactaaaa ataacgcctg ttttaataag cattagcgat
    16021 atgtattctg aagaaaaaat caagtttgtc gaaagttgtt taaatgagct tggagacatt
    16081 accgaagaac aattaaaaat tatgagattt gcaattataa aatctatacc aagggcaact
    16141 cttacagata aattagaaaa tgaattaaca aaagaaatat ataaaagctc atcgaaaatc
    16201 aacaattgct tgaacagaat taaattacca gagatgaaag aattctcatc aaaaaaaata
    16261 tatgattaca tcgatacaat cattgaagat tatgaaaata tcaaagaaaa tgcttatctg
    16321 gtcgtccccc aaattaatta tactatggat ttaaacatag aagactctag ctcagaagag
    16381 ttaataccct cgagaaagac gaaaattctc cggacaatgg ctttgaggtc ggggaatata
    16441 acacatatga agcatataac tcagagaatc aatattttac cagagaggac tatacgtata
    16501 attacgacct tttaaatgca atatagactt aataacttct tacaaagtct attattaata
    16561 ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa cttatcgacg cgggtttgaa
    16621 actgatggca accgcaaaaa tgcctgatgc gctacgctta tcaggcctac gccatctctg
    16681 caatatattg aatttgcgtg cttttgtagg caggataagg cgttcacgca gcatccggca
    16741 tgaataaagc gcactcaaca atctgaaacc cgccggaacg gtttattacg tacatcaggt
    16801 gaaactgacc gataagccgc tttcttttgg gtatagtgtc gtggacagtc attcatcttt
    16861 ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt acgtatatca ggtgaaactg
    16921 accgataagc cgctttcttt tgggtatagc gtcgtggaca gtcattcatc tttctgcccc
    16981 tccaaaagca aaaacccgcc gaagcgggtt tttacgtaaa acaggtgaaa ctgaccgata
    17041 agccgggttc tgtcgtggac agtcattcat ctaggccagc aatcgctcac tggctcaagc
    17101 agcctacccg ggttcagtac gggccgtacc ttatgaaccc ctatttggcc ttgctccggg
    17161 tggagtttac cgtgccacgg actgttacca gccgcgcggt gcgctcttac cgcacccttt
    17221 cacccttacc tgatcccgct tgcacgggcc atcggcggtt tgctctctgt tgcactggtc
    17281 gtgggtttcc cccccaggcg ttacctggca ccctgcccta tggagcccgg actttcctcc
    17341 cctccgcccg tctcccccga agaggacgac gacgaagcgg cgactgtctg gtcagcttcg
    17401 gcgcgcagta tagagggttt gcgcgccctt gtcaccccgt gttgcgcatt ccaatcgcca
    17461 gtgtcgcggc aatattacgt gaagcacggc agatattgtc ataagccccg cggaatgctt
    17521 cgtccaacgt acctatgctg gtcaatacgc tgaagaccgc atcaatgcca tgctgatgta
    17581 caacgccaac atcatcggtc aggctacccg caatgccaat caccggttta tggtacttct
    17641 tcgccacgtt tgcgacgcca atcggtacct tgccgtgaat actctggctg tcaatacgcc
    17701 cttcaccggt gatcaccagc gtacaatcgt gaatatgttc ctccagattc agcgccgtag
    17761 tgacgatttc aataccactt ttcagttccg caccaagaaa cgccatcagc accgcaccca
    17821 taccgcccgc agctcctgcg ccggggacat cttttacatc aacatgcagc gcttttttaa
    17881 tgacatcggc atagtgagag aggttattgt ccagctcaac aatcatcgct tcactggctc
    17941 ccttttgtgg gccaaagatg cgcgatgcgc cgttatcgcc caccagcgga ttggtgacat
    18001 cacaagcgac gcgaatgacg caatctttta agcgcggatc gaggccggaa atatcaatat
    18061 cattcagagt attaagacta ccgccgccaa aaccaatttc attgccgttg gcgtcgcata
    18121 atttcgcccc cagcgcctgt accatgcctg cgccgccatc atttgtagcg ctaccgccaa
    18181 tgccgataat aatgtttgtc gcgccgctct ccagcgccta caggattaac tcgcctgtgc
    18241 cgcgtgaagt ggtcacgagt ggatcgcgtt tttccgcagg taccagctcc agcccactgg
    18301 ccgccgccat ttcaataaac gcggttttgc catcgccgga gatcccccaa ctggcattca
    18361 ctttctcgcc cagcggccct gtaacccagg cgtgacgttc agaaccctgg gtggctgcaa
    18421 tcatcgcttc caccgttcct tcgccaccgt cggcaaccgg aatagaaacg tactgtgcat
    18481 caggaaaaat ttcccgaaat cctttttcta tcgcctgcgc aacctcgctg gcagataaac
    18541 tttctttata agagtctggg gcgattacga ttttcatacc tatgcctgtt accacatgac
    18601 gccggagggc gtttctctta ttcggcctgg attccaggcc cggattgcaa tacgccatcc
    18661 gggcacgacg tcattaacga gtaacttcga ctttcgccag tttttcgtag tagcacgcca
    18721 gggcgctatg atccgccgtt cctaaaccat ctgctcgcag tgcctgcatc atctccataa
    18781 ccgcagctgt gagcggcagt tgcgcgccga cgccgtggga agtatccagc gcatttgcca
    18841 gatccttaat atgcagatca atacggaagc ccggcttgaa gttgcggtcc atcaccatcg
    18901 gcgctttggc atccagcacg gtactgcccg ccagtccacc gcgaattgcc tgatacacca
    18961 ggtccgggtt aacgcccgct ttagttgcca gcgttaacgc ttctgacatc gcggcaatat
    19021 tcagcgccac aatgacctga ttcgccagtt tggtgacgtt acctgcacca attgccccgg
    19081 tatgcaccac ggaacccgcc attgctttca tcaaatcata gtatttgtcg aaaatagcct
    19141 tgtcgccgcc caccatcact gacagcgtac cgtcgatggc tttcggttca ccgccgctca
    19201 ccggagcatc cagcatatca atgcctttcg ctttcagcgc ttcgctgatt tcacggcttg
    19261 ccagcggtgc gatagaactc atatcgatca ataccgtacc tggcttcgcg ccttcaataa
    19321 tgccattctc acccagcgcc acctctttca catgagggga gtttggcagc atggttatga
    19381 tgacgtcgca ctgttcagcg atcgctttag ccgtagaggc tgtttctgca cctgcagcaa
    19441 tcacgtcagc aatagcttct gggttacggt cagcaaccac cagcgagtaa cctgctttca
    19501 gaaggttttt actcattggt ttacccataa tccccaggcc aataaaacca actttcatag
    19561 tcatatcaat catctctctt gttgcggtgg tggttatttt ttaaaggtat cagccagttt
    19621 atgagtggca gagcggaaga cgccgagatc gctgccgaca gccacaaacg tcgcgcccca
    19681 ttccagataa cgacgcgcat cggcttcgac cggcgcgagg ataccgctgg gtttgccgtg
    19741 cgcgctggca cggttaaaaa tgtgctgaat tgctttttgt acatccgggt gtgatgcatt
    19801 gccgagatgg cctaatgccg cggccagatc gctggggccg acgaagatgc cgtctacgcc
    19861 ttcggtagcg gcaatggcat cgacgttatc tacgccctgc tgactttcta tctggaccag
    19921 aatagtgatg ttcttgttcg actgagcgaa ataatccgcc acggtgccaa acatattggc
    19981 gcggtgagaa acggagacgc cgcgaatgcc ttccggtggg taacgggttg atgccaccgc
    20041 ctgctctgct tcctcttttg tttctacaaa aggaatcagg aagttataga aaccgatatc
    20101 cagaagacgc ttaataattt ccggctcgtt ggtcggcact cacactactg gcgcgctggc
    20161 gctgcctttc aaggccatta actgcggaat aaacgtggag atatcgtttg gcgcatgttc
    20221 gccatccagc accagccagt caaacccagc caaaccaaga acttcagtgc taatcgggtt
    20281 agagagtgct gaccagcaac caatttgtac ctgtttcgca gccagtgcgg ctttgaattt
    20341 attcgggaaa acatcgttat tcatcgctta tacctttgct tatttctgca attccatacg
    20401 tttaatgtcg ccaactacga agaggtagca gaccatcgcc atcagcgctg aacatcccac
    20461 gaaaaccagt gctgcattga aggagtgcag ttcacttacc aggtagccaa tcaccagtgg
    20521 agtgacaatg gaggcaacat tgccaaagac gttaaagacg ccgccacaga ggccaacaat
    20581 ctctttcggc gcggtgtcag aaatcaccgg ccagcccaga gcaccaaatc ctttgccaaa
    20641 aaaagccagc gccatcagca tgaccaccag cgtggtgttg ttggtgtagt tacataagat
    20701 gatggtggaa gccagcaaca ttcccagcac aatcggtagc ttacgtgcca gggtcaggga
    20761 taaaccgcgt ttgatcagat aatccgagaa gacacctccc agcacgccgc ccgcaaaacc
    20821 acacagtgcc ggaatcgagg cgaccagacc cactttcaga atcgacatgc ctttttcctg
    20881 caccagataa atcgggaacc aggtgaggaa gaaccaggtg atggtgttga taaaatattg
    20941 tccgaaaaat acgcccaaca tcatgcggtt agagagcaat tgcttgatgt aatgcagttt
    21001 gagaccgctt gctgccgcgc tgcccggctt tttgtggtcc atatcgacca ccgcgccatt
    21061 ttcagagata aacttcagct cttcctcaga catacgtggg tgatctgtcg ggttatgaat
    21121 caacttgatc cacagcgccg tcagcacaaa acaaatcacc cccataacgg taaagacgtg
    21181 ctcccatccc caggcgaaag tcagccagcc aagcagaggc gaaaagagcg ccagcgagaa
    21241 atattgtgcc gagttaaaga tggcggaggc agtaccacgt tctttcgtcg ggaaccaggc
    21301 ggcgacaata cgggcgttcg ccgggaatga tggagcttcc gagaatccca gcataaagcg
    21361 cataaagaac atggagatcc ctgcccaggc cagcgggaac atatcaacaa agccttgcag
    21421 gaaggtgaac agcgaccaga aaaagaggct gtaggtgtaa acttttttcg agccaaactt
    21481 atcaagcaac cagccgccgg ggatttgcat cagcaagtag gcccagccaa aagcggagaa
    21541 gatgtaaccc atcgaaaccg cacttaactg caactctttt gccacttcgg taccagcaat
    21601 agacagcgtt gcacgatcgg cgtagttaac ggcggtaaca ataaaaataa tcagtaatat
    21661 taaatagcgg gtatgcacac ctttcctttt ttcgtcaacg gtgtccagaa tcattttatt
    21721 tacctcgggt acttatgctg atttttatta ttatggggaa ggtgttattt ataagtttca
    21781 tttatgccgt aacgacaatg aactcgggaa ttagtataag cagcgcgaga ataataatca
    21841 ttgtgcaaat gctaatttaa ttaatacttt ttaaatatta ttttgagcat atgcacataa
    21901 ggttgcgcgc taaagcacag atttgcgctt taccttgccg ggcggcactg caatccctga
    21961 aatgattgac attgatcaca tttctgcgtt taaactcctg acattcttat ttcacccaat
    22021 gaaatcattt atttttaaat gagaccaggt cctcatttta ataacccctg gctggagaat
    22081 attgcacaat ggccaacatc gaaatcagac aagaaacgcc aactgcgttt tatataaaag
    22141 ttcacgacac agataatgtg gcaattattg ttaatgataa tggcctgaaa gcaggaacgc
    22201 gttttccgga tgggctggaa ttaattgaac atattcccca ggggcataaa gtcgcattgc
    22261 tggacattcc ggctaatggt gaaattattc gttatggcga agtgattggt tacgccgtgc
    22321 gtgcaatccc acgcggaagc tggatcgacg aatcaatggt tgtactaccg gaagcgccgc
    22381 cgttacacac gctgccactg gcaaccaaag tcccggaacc cttaccgccg ctggaaggat
    22441 acacctttga gggctatcgc aatgccgatg gcagcgtggg caccaaaaac ctgctcggca
    22501 tcaccaccag cgtccactgt gtggcaggcg tggtggacta tgtagtaaaa atcattgaac
    22561 gcgatctgct accgaaatac ccgaacgtcg atggcgtggt ggggctgaat catttgtacg
    22621 gttgtggcgt ggcgattaac gcaccggcag cagttgtacc tattcgtacc attcacaata
    22681 tttcgctgaa ccctaacttt ggcggcgaag taatggtgat tggcctgggt tgtgaaaagt
    22741 tgcagcctga gcgcctgctg actggaacgg atgatgtgca agctattcca gtagaaatcg
    22801 ccagcattgt cagtttgcag gatgaaaagc atgtcggttt tcagtccatg gtcgaggata
    22861 ttttgcaggt cgccgaacgc catctacaaa aactgaatca acgtcagcga gaaacctgtc
    22921 cggcttcaga actggttgtc ggcatgcatt gcggtggcag cgatgcgttt tctggcgtaa
    22981 cggcaaaccc ggcagttggc tatgcgtctg atctactggt gcgctgcggc gcaacggtga
    23041 tgttttcaga agtcacggaa gtgcgtgacg cgatccatct gctgacacca cgcgcagtga
    23101 acgaagaggt cggcaaacgg ctgctggagg agatggagtg gtacgataac tatctcaata
    23161 tgggaaaaac cgaccgcagc gccaaccctt cgccgggcaa caagaaaggc ggtctggcaa
    23221 acgtggtaga gaaagcgctc ggctccattg ctaaatcggg caaaagtgca attgttgaag
    23281 tgctgtcgcc cggtcaacgc ccgactaaac gcggattaat ttacgccgcg acgccagcca
    23341 gcgattttgt ctgtggcacg caacaggtgg cttcgggtat caccgtgcaa gtgtttacga
    23401 ccggtcgtgg tacgccgtac ggcctgatgg cggtacccgt cattaaaatg gcaacccgca
    23461 ccgagctggc gaaccgctgg tttgatttaa tggatattaa tgcgggcacc atcgctaccg
    23521 gcgaagaaac tattgaagag gtgggctgga agttgttcca ctttattctc gacgtcgcca
    23581 gcgggaagaa gaaaaccttc tcggatcaat ggggactgca taaccagctg gcggtgttta
    23641 acccagcacc ggtgacctga taattcaatt cgtattatgc ccgatagcac acgtttatcg
    23701 ggcatattga gtcacagcga agacgcgtga tccccgacca gaatcacttc aaccccagcc
    23761 tttcgcagtc cttccagact atccgcagga atgccttcat caacaatgat catgtcgata
    23821 cgttgagtat caatgatctt atgtaaactg gaacgattga atttactgga atcggtgacc
    23881 acgatgatcc gttccgcaac ttcgcacatc cgacggttta aacgggcttc atcttcatta
    23941 tgtgtgctga cgccgcgctc cagatcgatc gcatctacac caagaaacag catatcgaag
    24001 tggtaatttt gcagcgattg ctcagcctga tcgccgtaaa aagattgcga ctgacggcgc
    24061 aaatgcccgc cggtcatcag cagctcaacg ccttccgctt ccagcaacgc attagccacg
    24121 ttcataccgt tggtcatcgc aattacgtca gtgtacttgc gcatcagacg agcaatctca
    24181 aaagtggtgg tcccggaatc gaggatcacc cgatgacctg gctgaatcaa ctcaacggca
    24241 gctttcgcaa cgctgcgttt catcgcggtg ttcagtgcgc ttttatcttc cactgatggc
    24301 tcgactgacg gcgtcgtgct atcgcagatc aacgcgccac cataggcacg cacagcgatc
    24361 ccctgctttt ccagaaacgc cagatcattg cggatcgtca cagtagatac gccatacaat
    24421 gccgacagat cgttaacctg cacactccct tgctgtcgca gacgctgaat gatctgttct
    24481 cgtcgctcgc tggtgcctgt cactcgcttc tcacctgaag cgtcggtatt actcatagta
    24541 agtcctttcg taaaactttc gtttcatttc gttttgccta ttaacgcctt tctattaagc
    24601 aaatgcaagc ccaccttgcc cattggcgca agctactctc gtttcagtga ctttcattac
    24661 gtttcttttg tgaatcagat cagaaaacta ttatctttcg ttttattttt atctcaccat
    24721 gacgcagtat caattgaaac aaaacgaaag atcaatatcg cagtaatctg aactggagag
    24781 gaaagtgaaa catctgacag aaatggtgag acagcacaaa gcgggcaaaa caaatgcaat
    24841 ttatgccgtt tgttccgcac atccgctggt gctggaggct gcaatccgct acgccagtgc
    24901 aaaccaaacg ccgttactga ttgaagcaac ctccaatcag gtagaccagt tcggcggtta
    24961 taccggaatg acgcccgccg attttcgcgg ctttgtttgt cagctcgccg actcgttgaa
    25021 tttcccgcag gatgcgctga ttctgggtgg tgaccatctg gggccaaacc gctggcaaaa
    25081 cctgccagcc gctcaggcaa tggccaatgc cgatgatttg attaaaagct acgttgcggc
    25141 aggattcaaa aaaatccacc ttgattgcag catgtcctgt caggacgatc caattccctt
    25201 aactgatgac atcgtggctg aacgcgccgc ccgtctggcg aaagtggcgg aagaaacctg
    25261 tcgcgaacac tttggcgaag ccgatctgga gtatgtcatt ggtaccgaag tgccggtacc
    25321 tggcggcgcg catgaaacct taagcgagct ggcggtcacc acgccggatg ccgcccgcgc
    25381 cacgctggaa gcccatcgtc acgcctttga aaagcaaggt ttgaatgcca tctggccacg
    25441 cattattgcc ctggtggttc aacccggcgt cgaattcgat cacaccaacg ttattgatta
    25501 tcagcccgcc aaagcgagcg ccttaagcca gatggtcgaa aactacgaaa cgctgatttt
    25561 cgaagcgcac tctaccgatt atcaaacgcc gcaatctctg cgccagctgg tgattgacca
    25621 ctttgccatt ctgaaagttg gcccggcgct gaccttcgcc ctgcgtgaag cccttttttc
    25681 tctggcggcg attgaagaag aactggtacc agcgaaagcc tgttccggtc tgcgtcaggt
    25741 gctggaaaac gtgatgctcg accgcccgga atactggcaa agccactacc acggtgacgg
    25801 caacgcgcgt cgtctggcgc gtggttacag ctactcagat cgcgtgcgct attactggcc
    25861 ggacagccag attgatgacg ctttcgctca tctggttcgt aatctggcgg attcaccaat
    25921 tccgctaccg ctcatcagcc agtatctgcc gctgcagtac gtgaaagttc gctccggcga
    25981 gctgcaaccg acgccacggg aactcattat aaaccatatt caggacatcc tggcgcagta
    26041 ccacacagcc tgtgaaggcc aataagcaaa acaaagagga acatgctatg ccaaatattg
    26101 ttttaagccg gattgatgaa cgcttgattc acggtcaggt cggcgttcag tgggtcggat
    26161 ttgcgggggc aaatctggtg ctggtagcca acgatgaggt tgccgaagat ccggtacaac
    26221 aaaacctgat ggaaatggta ctggcagaag ggatcgccgt acgtttctgg acgctgcaaa
    26281 aagttatcga caacattcat cgcgccgccg atcgacagaa aatcctgctg gtttgtaaaa
    26341 cacccgccga tttcctgacg ctggtgaaag gtggcgttcc ggtgaatcgc attaacgttg
    26401 gcaatatgca ctacgccaat ggcaaacaac aaatcgccaa aacggtttct gtggatgcgg
    26461 gcgatatcgc agcatttaac gacctgaaag ccgctggggt ggaatgcttc gttcagggcg
    26521 tcccgacaga gcctgctgtg gacctcttta aattactttg agggattcat catggaaatc
    26581 agcctgttgc aggcatttgc gttgggcatt atcgccttta tcgctggcct agatatgttt
    26641 aacggcttaa cccatatgca ccgcccggtg gtcctcggcc cgttggtcgg gctggtactt
    26701 ggcgatctgc ataccggaat tttaaccggc gatacgctgg aactagtgtg gatggggctg
    26761 gccccgctgg cgggcgcaca gccgcctaac gtgattatcg gtactatcgt cggcacggcg
    26821 tttgccatta ctactggcgt gaaacccgat gtcgcggtcg gtgtcgccgt acctttcgct
    26881 gtcgcagtac agatggggat taccttcctg ttctcggtga tgtctggcgt gatgtctcgc
    26941 tgcgaccgga tggcggagaa cgccgatact cgcggcattg aacgcgtgaa ctatctggca
    27001 ctgctggcac tcggcacttt ctattttctc tgcgctttcc tgcctatcta cttcggcgcg
    27061 gaacatgcaa aaaccatcat tgatgtcctg ccgcaacgat taatcgacgg cctcggcgtc
    27121 gcgggcggca tcatgccagc aatcggtttt gccgtgctgc tgaaaatcat gatgaaaaac
    27181 gtctacatac cctacttcat cctgggcttt gttgccgcaa cctggctcaa gttaccggtg
    27241 ctggctattg ctgccgccgc gctggcgatg gcgctgatcg atctgctgcg taaatctcct
    27301 gaaccgactc aacctgcggc acagaaagag gaatttgaag atggcatcta atcaaaccac
    27361 cctgccgaac gtcagcgaaa atgaagaaac actgctgact ggcgtcaatg aaaacgtgta
    27421 tgaagatcgg agcattgccg cggagctgac gaaaaaagat atcaaccgcg tcgcctggcg
    27481 ttccatgctg ttacaagcgt cttttaacta cgaacgtatg caggcttctg gctggttgta
    27541 cggtctgctg cctgctctga aaaagatcca cactaataaa cgcgacctgg cgcgcgccat
    27601 gaaggggcat atgggtttct tcaataccca tccgtttctg gtgacgtttg ttatcggcat
    27661 tatccttgcg atggagcgtt ctaagcagga cgttaacagt attcagagca ccaaaattgc
    27721 cgtcggtgcg ccgcttggcg ggattggcga tgcgatgttc tggctaacgc tactgccgat
    27781 ttgtggcggg ataggcgcaa gcctcgcgct acaaggctct attcttggcg cagtcgtctt
    27841 tattgtgctg ttcaacgtgg tacacttggg gctgcgtttt ggtctggcgc attatgctta
    27901 ccgcatgggc gtggcggcga ttccccttat taaagcgaat accaaaaaag tcggccatgc
    27961 tgcgtcaatc gttggaatga cggtaatcgg cgcactggtg gcaacctatg ttcgtttaag
    28021 caccacgctg gaaatcacgg cgggcgacgc agtagttaag ttacaggctg atgttatcga
    28081 caaactgatg cctgccttct taccgctggt ctacaccctg accatgttct ggctggtacg
    28141 ccgcggctag agtccgctgc gcctgattgc ggttaccgtg gttctaggga tcgtcggtaa
    28201 attctgccat ttcctttaaa tacaatgagg tattagatgt taagtattat tttgacaggg
    28261 catggcgggt ttgccagtgg catggaaaaa gcaatgaagc aaatcctcgg cgatcaatcg
    28321 cagtttattg ccatcgattt tcccgaaacc tcaagcgccg cgctgcttac atcgcagctt
    28381 gaagaggcca tcgcccaact ggattgtgaa gacggcattg tttttctgac ggatttgctg
    28441 ggcggcacgc cgtttcgcgt agcttcaacg ctggcgatgc aaaaaccggg ctgtgaagtg
    28501 attaccggca ccaatttgca actgctactg gagatggtgc tggagcgcga agggttaagc
    28561 ggtgaagaat tccgtgtaca ggcgctggag tgtggacacc gagggctgac cagtctggtg
    28621 gacgagttag gccgctgcca tgaagaatgt ccggtcgagg aaggaatatg acacacgttc
    28681 tgcgcgccag aaggctgctg actgaagagg gatggttcga tgaccatcag ttgcgtgttg
    28741 ctgacggtgt catcgcagca atcgaaccga ttccagtggg cgtgactgaa cgcgatgcgg
    28801 aactgctctg ccccgcttac atcgacaccc atgtacacgg tggtgcgggc gttgatgtaa
    28861 tggatgacgc gccggatgta ctcgacaagc tggcaatgca caaggcacgc gaaggtgtcg
    28921 gcagttggtt accgaccacc gtaaccgcgc cgcttaatac cattcatgcg gcgctgaaac
    28981 gtattgctca acgttgccaa cgcggcggac ctggtgcgca agtgctgggg agttatctcg
    29041 aaggaccgta cttcacgccg cagaataaag gcgcgcatcc
//
LOCUS       DAJVRL010000049        29048 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVRL010000049 DAJVRL010000000
VERSION     DAJVRL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.049"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            108..1139
                     /gene="mdtN"
                     /locus_tag="OQN02_002113"
     CDS             108..1139
                     /gene="mdtN"
                     /locus_tag="OQN02_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1642899.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1139..3187
                     /gene="mdtO"
                     /locus_tag="OQN02_002114"
     CDS             1139..3187
                     /gene="mdtO"
                     /locus_tag="OQN02_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1642900.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3184..4650
                     /gene="mdtP"
                     /locus_tag="OQN02_002115"
     CDS             3184..4650
                     /gene="mdtP"
                     /locus_tag="OQN02_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1642901.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4848..5087
                     /locus_tag="OQN02_002116"
     CDS             4848..5087
                     /locus_tag="OQN02_002116"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642902.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5097..5267
                     /locus_tag="OQN02_002117"
     CDS             5097..5267
                     /locus_tag="OQN02_002117"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1642903.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5316..6728
                     /gene="fdhF"
                     /locus_tag="OQN02_002118"
     CDS             5316..6728
                     /gene="fdhF"
                     /locus_tag="OQN02_002118"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS1642904.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6729..6995
                     /locus_tag="OQN02_002119"
     CDS             6729..6995
                     /locus_tag="OQN02_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCS1642905.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7089..7778
                     /locus_tag="OQN02_002120"
     CDS             7089..7778
                     /locus_tag="OQN02_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1642906.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="OQN02_002121"
     CDS             complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="OQN02_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1642907.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="OQN02_002122"
     CDS             complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="OQN02_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1642908.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="OQN02_002123"
     CDS             complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="OQN02_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1642909.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10480..12198)
                     /locus_tag="OQN02_002124"
     CDS             complement(10480..12198)
                     /locus_tag="OQN02_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1642910.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="OQN02_002125"
     CDS             complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="OQN02_002125"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1642911.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="OQN02_002126"
     CDS             complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="OQN02_002126"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1642912.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="OQN02_002127"
     CDS             complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="OQN02_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1642913.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="OQN02_002128"
     CDS             complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="OQN02_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1642914.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16279..18237
                     /gene="acs"
                     /locus_tag="OQN02_002129"
     CDS             16279..18237
                     /gene="acs"
                     /locus_tag="OQN02_002129"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCS1642915.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18437..18751
                     /locus_tag="OQN02_002130"
     CDS             18437..18751
                     /locus_tag="OQN02_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCS1642916.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18748..20397
                     /gene="actP"
                     /locus_tag="OQN02_002131"
     CDS             18748..20397
                     /gene="actP"
                     /locus_tag="OQN02_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1642917.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20575..21867
                     /locus_tag="OQN02_002132"
     CDS             20575..21867
                     /locus_tag="OQN02_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1642918.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22021..23670)
                     /locus_tag="OQN02_002133"
     CDS             complement(22021..23670)
                     /locus_tag="OQN02_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1642919.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="OQN02_002134"
                     /gene_synonym="yjcD"
     CDS             complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="OQN02_002134"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1642920.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMVGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="OQN02_002135"
     CDS             complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="OQN02_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1642921.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26426..26749
                     /gene="soxS"
                     /locus_tag="OQN02_002136"
     CDS             26426..26749
                     /gene="soxS"
                     /locus_tag="OQN02_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1642922.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="OQN02_002137"
                     /pseudo
     CDS             complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="OQN02_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28758..29039
                     /locus_tag="OQN02_002138"
     CDS             28758..29039
                     /locus_tag="OQN02_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCS1642923.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7289 a         7777 c         7348 g         6634 t
ORIGIN      
        1 gggcaaatat caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg
       61 ccatgctgtt ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc
      121 cgaaaaaagc acctcgcagt aaattccctg ctctgttagt ggttgcgttg gcgctggttg
      181 cccttgtttt cgttatctgg ctcgtagaca gtgcgccatc aactaatgac gcttacgcgt
      241 cagcagatac cattgatgtg gtgccggaag tcagcggtcg cattgtagaa ctggcggtca
      301 ccgacaacca ggcggtgaaa cagggcgatt tgctgttccg catcgacccg cgcccgtacg
      361 aagccaacct ggcgaaagcc gaagcctccc tcgcggcgct ggataagcaa attatgctca
      421 cccagcgtag cgttgacgcc caacagtttg gtgccgactc ggttaatgcc acggtagaaa
      481 aagcccgtgc cgccgcgaaa caggccacag atacattacg ccgcaccgag ccattactga
      541 aagagggttt tgtctcagca gaagacgttg accgtgcaag aacggcgcag cgcgccgcag
      601 aagcggatct taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg
      661 tcgatgcgct ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactac
      721 atctggaaat gacgaccgtt cgcgcgccat ttgatggccg gatcatttcc ctcaaaacct
      781 ccgtcgggca atttgcttct gccatgcgcc ctatttttac cctcatcgac actcgtcact
      841 ggtacgtgat cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg
      901 caacgattcg cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg
      961 gctacggcgt gctaccggat gacggcggcc tggtgctggg cggcctgcca aaagtgtctc
     1021 gttctattaa ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac
     1081 ctgacccgga aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat
     1141 gagcgcgctc aactccctgc cattaccggt ggtcaggctg ctggcgttct ttcatgaaga
     1201 gttaagcgag cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct
     1261 gctggtgatt ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt
     1321 gctgttttac ggtatcggta ttcagtcgaa cgcgttttac accaaatttg tggcgatctt
     1381 gtttgtggtt gccacggtgc tggagatcgg cagcctgttt ttgatctaca aatggtcata
     1441 cggcgaaccg ttgatccgat tgatcatcgc cgggccgatc ctgatgggct gcatgttttt
     1501 gatgcgcacc catcggctgg gactggtctt tttcgctgtc gccatttacg ggcaaacctt
     1561 ccccgccatg ctcgactatc cggaagtagt cgtgcgctta acgctgtggt gtatcgttgt
     1621 tggcctctat ccaaccttgc tgatgacgtt aatcggcgtg ctgtggtttc ccaatcgtgc
     1681 cattacgcaa atgcatcaag cgcttaatga tcggcttgat gatgccatta gccacctgac
     1741 ggacagcctc gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg cgctgcaaaa
     1801 actgaatgtc ttttgtctcg cggacgatgc caactggcga actcaaagcg catggtggca
     1861 aagctgcgtg gcaacggtaa cctacattta ctcgacgctg aatcgctacg atcccacctc
     1921 ttttgctgat tctcaggcaa ttattgaatt tcggcaaaaa ttagcttcag aaatcaacaa
     1981 gctgcagcat gccgttgctg aaggtcagtg ctggcaaagc gactggcgga tcagtgaaag
     2041 tgaagcggtg gcggcacggg aatgtaacct ggagaatatc tgccagacgt tgttacaact
     2101 gggccagatg aacccgaata cgccgccaac gcccgccgcc aaaccgccat caatggtcgc
     2161 cgatgctttt accaatccag actatatacg ctacgcggta aaaacattgc tcgcctgttt
     2221 gatctgttac accttttaca gcggcgtgga ctgggaaggc attcacacct gtatgctgac
     2281 ctgcgtgatc gtcgctaatc caaatgtcgg ttcgtcgtac cagaagatgg tgctgcgttt
     2341 tggcggggcc ttttgcggcg cgattctggc gctgttattc acgctactgg tcatgccctg
     2401 gctggacaat attgtcgaat tgctgtttgt gctggcaccg attttcctgt tggccgcatg
     2461 gattgccacc agctctgaac gctcttctta tatcggcaca cagatggtgg tcaccttcgc
     2521 gctcgccacg ctcgaaaacg tttttggccc agtgtacgac ctggtggaaa ttcgcgatcg
     2581 cgccctgggt atcatcattg gtaccgtggt gtccgcgatg atttacacct ttgtctggcc
     2641 tgaaagtgaa gcgcgcacgc tgccgcaaaa actggctggc gcgctgggta tgttaagtaa
     2701 agtaatgcgg atcccacgcc agcaggaagt cacggctctg cgcacttatc tgcaaattcg
     2761 tatcggtctg catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg tggcgctgga
     2821 gcgtcaactg gacagcgaag aacgcgcctt actgattgaa cgttcgcaaa cggttattcg
     2881 tcagggccgc gatatccttc acgcctggga tgccacctgg aattcggcgc aggcgctgga
     2941 taacgcacta cagccggaca gagcaggtca gtttgccgac gccctggaga aatacgctgc
     3001 cggtctggca accgcactca gccgttctcc tcaaataacg cttgaagaga cacccacctc
     3061 gcaggccatc ctgcccacct tattaaaaca ggagcaacac gtctgccagc ttttcgcccg
     3121 cttgccagac tggacagccc cggcattaac gcccgccacg gaacaggcac aaggagccac
     3181 gcaatgatca atcgtcaact ttcacgtctg ctgttgtgca gcattctcgg cagcacgacg
     3241 ctgatttccg gctgtgccct ggtacgtaag gattctgcgc ctcatcaaca gctcaaaccg
     3301 gaacaaatca aactggccga tgatattcat cttgccagct ccggctggcc gcaggcgcag
     3361 tggtggaaac aactcaatga cccgcagttg gatgccctga tccaacggac gctaagtggt
     3421 tcacacaccc tcgccgaagc gaaactgcgg gaagaaaaag cgcaatcgca ggccgatttg
     3481 ttagatgccg gttcacagtt gcaggtggca gcgttaggga tgcttaaccg ccaacgtgtc
     3541 tcggcgaacg gctttttaag cccttatgcg atggatgcgc ccgcactggg gatggacggg
     3601 ccgtactata cggaagccac agtaggtttg tttgccggac tggatcttga tttgtggggt
     3661 gtgcatcgct cagcggttgc cgccgccatt ggcgcgcata atgccgcgct ggcagaaacc
     3721 gcagcagtag agctatcgct gaccacgggc gtagcgcagc tttattacag tatgcaggcc
     3781 agctatcaga tgctcgatct gttagaacaa actcgcgatg tgattgatta cgcggtgaaa
     3841 gcgcaccaaa gtaaagtggc gcacggtctg gaagcacaag tgcctttcca cggcgcgcgg
     3901 gcacagattc tggcggtcga taaacaaatt gctgccgtca aagggcaaat caccgaaacg
     3961 agagaatctc tgcgcgcatt gattggcgcg ggagccagcg atatgccgga gatcaaacct
     4021 gtggcattac cacgagtcca gaccggcatt ccggcgacac tctcttatga gttgctcgcc
     4081 agacgcccgg atctgcaagc catgcgctgg tatgttcagg cgtcattaga tcaggtggat
     4141 tccgcgcggg cgttgttcta tccgagcttt gatatcaaag cgtttttcgg ccttgatgcc
     4201 atccacctgg ataccttatt caaaaaaacc agtcgccagt tcaacttcat cccgggtctg
     4261 aaattgccgc tgtttgacgg tggacggttg aatgccaatc tcgaaggcac gcgcgccgcc
     4321 agcaacatga tgattgaacg ttacaaccag tcagtactga acgcggtgcg tgacgttgcc
     4381 gtcaacggca cgcgtctgca aacgctcaac gacgagcgag aaatgcaggc tgaacacgtg
     4441 gaagcaacgc gcttcaccca gcgcgctgcc gaggccgcct atcagcgcgg cttaaccagc
     4501 cgcttacagg ccactgaagc ccggttgcca gtacttgccg aagagatgtc attactgatg
     4561 ctggacagcc gccgggtgat ccaaagcatt cagttgatga aatcgctggg cggcgggtat
     4621 caggcggctc ccgtcgtcga gaaaaaataa aatgtctgcc gcgtgatggc tgtcacgcgg
     4681 tatttcgttt cgtcacgtca aaactgacga cagcctgttt ttcgtcagag ttttgaataa
     4741 atagtgcccg taatatcagg gaatgacccc aaataaaatg tggcataaaa gatgcatact
     4801 gtagtcgaga gcgcgtatgc gtgatttgat taactggagc gagaccgatg aaaaaagtcg
     4861 tcacggtttg cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc gataacggca
     4921 aaatcgtccg ggcggaggca gcgcagggga aaaccaacca gggtaccctg tgtctgaagg
     4981 gttattatgg ctgggatttc attaacgata cccagatcct gaccccgcgc ctgaaaaccc
     5041 ccatgatccg tcgccagcgt ggcggcaaac tcgaacctgt ttcctgagat gaggcactga
     5101 attacgttgc cgagcgcctg agcgccatca aagagaagta cggtccggat gccatccaga
     5161 cgaccggctc ctcgcgtggt acgggtaacg aaaccaacta tgtaatgcaa aaatttgcgc
     5221 gcgccgttat tggtaccaat aacgttgact gctgcgctcg tgtctgacac ggcccatcgg
     5281 ttgcaggtct gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt aacgaaattg
     5341 ataataccga tttagtgttc gttttcgggt acaacccggc ggattcccac ccaatcgtgg
     5401 cgaatcacgt aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc gatccgcgca
     5461 aaattgaaac cgcgcgcatt gctgacatgc acattgcact gaaaaacggc tcgaacatcg
     5521 cgctgttgaa tgcgatgggc catgtcatta ttgaagaaaa tctgtacgac aaagcgttcg
     5581 tcgcttcccg tacagaaggc tttgaagagt atcgtaaaat cgttgaaggc tacacgccgg
     5641 agtcggttga agatatcacc ggtgtcagcg ccagtgagat tcgtcaggcg gcacggatgt
     5701 atgcccaggc gaaaagcgcc gccatcctgt ggggcatggg tgtaacccag ttctaccagg
     5761 gcgtggaaac cgtgcgttct ctgaccagcc tcgcgatgct gaccagtaac ctcggtaagc
     5821 cgcatgcggg tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc tgcgatatgg
     5881 gcgcgctgcc ggatacgtat ccgggatacc agtacgtgaa agatccggct aaccgcgaaa
     5941 aattcgccaa agcctggggc gtggaaagcc tgcctgctca taccggctat cgcatcagcg
     6001 agctgccgca ccgcgcagcg catggcgaag tgcgtgccgc gtacattatg ggcgaagatc
     6061 cgctgcaaac tgatgcggag ctgtcggcag tacgtaaagc ctttgaagat ctggaactgg
     6121 tcatcgttca ggacatcttt ataaccaaaa ccgcgtcggc ggcggatgtc attttgccgt
     6181 caacgtcgtg gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc ttccagcgtt
     6241 tcttcaaagc agttgaaccg aaatgggatc tgaaaacgga ctggcaaatc atcagtgaaa
     6301 tcgccacccg tatgggttat ccgatgcact acaacaacac ccaggagatc tgggatgagt
     6361 tgcgtcatct gtgcccggat ttctacggtg cgacttacga gaaaatgggc gaactgggct
     6421 tcattcagtg gccttgccgc gatacttcag atgccgatca ggggacttct tatctgttta
     6481 aagagaagtt tgataccccg aacggtctgg cgcagttctt cacctgcgac tgggtagcgc
     6541 caatcgacaa actcaccgac gagtacccga tggtactgtc aacggtgcgt gaagttggtc
     6601 actactcttg ccgttcgatg accggtaact gtgcggcact ggcggcgctg gctgatgaac
     6661 ctggctacgc acaaatcaat accgaagacg ccaaacgtct gggtattgaa gatgaggcat
     6721 tggtttgagt gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc agcgatcgtc
     6781 cgaacaaagg ggcgatttac atgacctacc agtggtggat tggtgcctgt aacgagctgg
     6841 ttaccgaaag cttaagcccg attacgaaaa cgccggaata caaatactgc gccgtgcgcg
     6901 tcgagccgat ctccgatcag cgcgccgccg agcagtacgt gattgacgag tacaacaagt
     6961 tgaaaactcg cctgcgcgaa gcggcactgg cgtaatacag tcctttctac agcctccttt
     7021 cggaggctgt ttttttatcc attcgaactc tttatactgg ttactcccta cccaatcgta
     7081 ttatcaaaat gaaaaaaatt atcgcattga tgttgttttt gacattcttt gcccacgcga
     7141 acgactccga gcctggcagc cagtatttaa aggcagcaga ggccggggac cgacgtgcac
     7201 aatattttct tgccgacagc tggtttagct ccggcgattt gagcaaagcc gaatattggg
     7261 cacagaaagc cgccgacagc ggtgatgctg atgcctgcgc gctgttggcg cagatcaaaa
     7321 tcaccaatcc ggtcagtctg gactatccac aagcaaaagt tcttgcagag aaagcggcgc
     7381 aagcgggcag taaagaaggt gaagtaacgc tggcgcatat tctggtaaat actcaagcgg
     7441 gtaaaccgga ttatccaaag gcgatttcgc tgttagaaaa cgcctcggaa gatctggaga
     7501 atgactccgc cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac ggcgtgggca
     7561 ttaaggccga cgatgacaag gcaacctggt atttcaaacg cagctctgca atttcccgaa
     7621 ccggttattc cgagtactgg gcgggaatga tgttcttaaa cggtgaggaa ggttttatcg
     7681 agaagaacaa acaaaaggcg ctgcactggt tgaacctgag ctgtatggaa gggtttgata
     7741 ccgggtgtga agagtttgaa aaattaacca acggttaagg taggcctgat aagacgcggc
     7801 aagcgtcgca tcaggcatcc acacattgcc gggggatatc cccggcaatc ttcaattatt
     7861 gatccgcagt tttatcaaat ttgcccagca cttcacgctc ataagccagc gcctttttgc
     7921 ggtcaaattt gtgttcccac ttggcaatca ccagcaccgc cagcgcatta cccaccacgt
     7981 tcagcgcagt acgcgccatg tcgaggatac ggtcaacacc agcaataaac gccagacctt
     8041 ccagcggaat acctacgcta cccagcgttg ccagcaacac cacaaacgac acgccagcca
     8101 cgccagcaat ccctttcgag gtcaccatca gcgtcagcac cagaatgatt tcctgccaga
     8161 tggacaggtc aatgccatac agctgagcga tgaaaatagc ggcaatactt tgatacagcg
     8221 tcgaaccatc aaggttaaaa gagtaaccgg tcggcaccac gaagctggtg atcgacgccg
     8281 gtgctccgta ggcttccatc ttctcaataa ttcgcggcag tacgctttca gagctggcag
     8341 tggagtacgc cagaatcagc tcatctttca gaatacgaat caggatccag acgcttaacc
     8401 cgcacagacg tgccacaatt cccagcacta ccagcgcgaa gaacagaatg gcgaaatgca
     8461 ccagcagcac cagtttcgcc agtggccaca gagacgagaa accaaagtta gccaccgtca
     8521 ccgcaatcag cgcaaacaca cccaccggtg cataacgcat caccatgtga gtcactttaa
     8581 acatggtttc agagatggag cggaacacgg tcaccagcgg ttcacgatgc gtcgcgggca
     8641 gggaagaaag ccccagacca aacagcaccg agaaaaagat gatcggcagc atttcgcctt
     8701 tcgccatcga cgccacaatg ttcgtcggca ccagcgacaa aatcgtgccc ataatgccgt
     8761 gggaactgct ttgtaccgct tccgtagtgc tctgatattt cgagatatcg acggtcgcca
     8821 gctgcgacat atccaccccg gcaccgggct ggaagacgtt cgccagagtg atccccaaaa
     8881 tgatggcgac ggtggtgatc acctcgaagt aaataatggt tttcgcgcca atacgcccga
     8941 gctgtttggc atcaccaaca cccgcgatac ccaccaccag cgtggagatc acaatcggca
     9001 caacaatcat tttgatcaga tggatgaaga tatcacccgc cggagagagc aaattgacga
     9061 ccagccagtc gcggctgtcg ctatggtagt gcaggtagct tcccaggaga atgcccagca
     9121 ccatagcaaa cagaatctgc caggccaggc tgaattttat atttttcatc agaataactt
     9181 cctcaatgga gcgccgttcc ccgttcagaa acgcgagaac gtgacatact gccagggcat
     9241 gaattgtgtt gcgttggttt gtagattttt ttaacgctta tgatcagtat catttgcgcg
     9301 gtatgttgcg caacccttta acaacagagc ttttcgctgc attaatgcat aataacgcga
     9361 gtttatggca ggattaatga ataaccattt attatggaaa gatttttatt tgtttaatct
     9421 gcaataatac tgattggtga ttatttttgg catatgttca ttcgattatt ttttatgcag
     9481 ctcaatgaca taatcttcat caccagatga ttaatccgat ctccgctgca acagttttgc
     9541 catattaatc gactcgacca gctgtgtccg gttgatccgg ggtgagttga gatccatcac
     9601 tttttgccat agttcgatgg cttgcgcgta attcgcctgc ataaacgcat cggaagccag
     9661 cagcatcaga gcggtgattt cgttagagtc cagcgcgagg gctttgtcga tcattgcgcg
     9721 agtctgggcg gtcatatgct ggctggcctg gtaataaagc accgtcgcca gcgccgcata
     9781 cagttcagcg ttctcaccac gtagttgcaa cgcctgacga tacgccagca gcgaattgct
     9841 gtaatcgttt tgccacagat agtactcgcc cagtaacgcc cattgttcgc tgttttgtgg
     9901 attagcgcgg attttatcct gcaatgcctg aagctgcgct tccggggttt gctggctggc
     9961 aaactgatgt agcggatcgc gctgacgctg atactccgca cgtaccgcct gccattttgg
    10021 gcttaacaga taactgccga cacagaggaa taccatcaat atcgtgagtg ttgtcagcat
    10081 tttcaccggt atttgaggct gtttcatcgc ttagccctca ctcgccagag gatcagtgcc
    10141 atcagcaata acaacaccac tggcagcgcc cacaacacca gcgtctgacc cgttaacggt
    10201 gggttatagc gaacaaaatc tccgtagcgt tcggtcatcc agccaatgat ttcgacttcg
    10261 ttcttcccct ccgacaccat gctataaacc tgatggcgca tactgaccgc caccggcgca
    10321 ttggattcga gtaagttttg attctggcac tgcggacaac gtaactggct ggcaatattt
    10381 aacgcctgtt gctgttgttg cggattggcg aattgccagg tgtctacaac ctgagcgcgc
    10441 gcaaaacagg taaataacag cagtagcgtg agaagccctt tattcatcgc gcttcttccc
    10501 ccgccatccg cttaacaatg cgcccgcaat catcaacaat cctccccccc agatccagcg
    10561 cacaccgctt tgtacataca aacgaaaagc gtaacgatcc gctctggttt tttcacccat
    10621 gaccgcatac cagtcatgga tgccgttcca gcgaattgac ggttccatca tttgctgacg
    10681 gcgtgcttca taaaaacgcc gctccggcgt taattcacca atgcgttgct gatggtcaaa
    10741 cagcgccact atcgcttttt cgctggtgta attgcctctg gcttgcagat cgagtcgctc
    10801 aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat
    10861 ctcctgacga ctgacactgg aaaccacgat ccccgcggca aataacagca cgccagcatg
    10921 agctaccagc gccggaagct gcacgcgttt gccagagaca aacgtcgcca gcacaatcac
    10981 caccagcatc aacagaccaa acggtaacgt cgcgcggtta aaatacggcg cgccaacgga
    11041 gaggcgtccc cagcccagca ggccgtaaat catcggataa agcgttccca ccagcacgat
    11101 cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt aacatttccc gcgataaccc
    11161 cgaaaaacgc accgccgggc caccgtcccg cgctcgccag ccatacagag ccaaagacgc
    11221 aaggctaatc agtgcaaaca ggctgaacaa cggcacggcg cggacgttat ccagtgcgaa
    11281 cgcatgaacc gaaaccagaa tgccagaacg gacaattaag gtgcccagca gcgacagcat
    11341 cagagtaact atcgccagca acagcgacca gtggcggaaa atcccccgct ggcgtgtcag
    11401 ggacaaactg tgcagcagcg cagtggcaga aagccagggt aataaagagg cgttttccac
    11461 cggatcccag aaccaccagc cgccccagcc gagttcgcag taggcccacc aggaaccgag
    11521 gatgatcccc gccgttaatg cactccagcc aggtaacgcc cagcgccagc aaattcgggc
    11581 gctggccgca tcaaactcgc cgcgcagtag actcgccagc gccacgctcg ccgctaccat
    11641 caaaccgcca tagccaaggc aaagcagcgg tggatgaaag ataagaccgg gatgttgcag
    11701 catcggattg agatcgcggc cttcgattgc tggtggaaat atccgcacga agggatcgga
    11761 ccacagcacc acaaacagta gcagtgcggc gagcatgaga gataaaacgg ctagcgtcag
    11821 cggaaatagc ggatcgcttt gctgccgata atgccaggca aacagcgcgc tccaggcgga
    11881 aagcagcagc acccaaagca gcagcgaacc ttcatgaccg ccccacaccg ccgccagttt
    11941 gagttcccac gacagcaggc tgtagctatg ttgggcgaca taaatgaccg agaaatcgct
    12001 gatgagaaaa caatacgtca gtacgccaaa ggcgagcagc aggagcgcga actgcgccag
    12061 aatgccgatg caagtgagtc gcatcatggc aggccagcgc aaccgcactc ccgcaaaggc
    12121 cgtcaacggg gtcaacacgt tgaccccgag acttaacaac aacgccagaa aaccgacttc
    12181 aggaaggaat acgtccagaa accacctctt ttctggctta cgccaccgtc aactgtccgg
    12241 cgtagagaat gaaaaagcgc agcatcaaca cgccgaccag actggcccca cacgccgcca
    12301 gcacggcagg aatgccggaa ctgcgattga cccacggttt gagcaacatt ggcacaatca
    12361 gccccagccc ggcgacacca agccagaacc accacgtcca gaaaccgcca cctaatgccg
    12421 ccaccagcgc acgaacttta ccgtcatcgc ccagcgccag accgacaaaa aacgccacca
    12481 gcaggaagat ttcaccccat accacgggga tttccatccg gtgaacaaag tgcgcttccg
    12541 tggaatgcgg gttactgcgt tggcgtatcg ccatggcgat cagcgccacc gccgcaccgg
    12601 acgagatgcc ggagaagagg agcagcaccg gcaggctcgg gttattgagg aacggatacg
    12661 atttcagcgc cgacagcaga aagccggtat aagcccccag cagcaccgcc aacaccagca
    12721 tcagcgtttc cagtccgcga tgaacgggcg tcagcaggct cagaaccttt tgcacgatcc
    12781 ccagcttcgg caaccagcgt tgttgcaggg caagcaaatc atgttcaaag attttcgcca
    12841 gccacagcac cagcaccacc atgtagagct gaaacagcat cacccccatc gacatcaccg
    12901 aggtaaaact gtagtggaac atcagcttcc agaaggtcca cggtcttgtc aggtggaaga
    12961 cgaggatcaa cagaccgagg atcaccgcgc ccggcccgac aatcagcgtg gtgcgcagca
    13021 acgtactgtc tgcaccgccc gcctgcgggt agaagcgacg taacagcacg gccagcgtca
    13081 ccagaccggc agaaatacca atcaaaaaca ggtagatggc aatcggccag tcccacacca
    13141 gcgattcaaa atgaaatgcg gaagtctgcg tcattggctc acctccccgt atttaaacgg
    13201 tacgcggtac agcttcggtt tggttcccag cgccagcttg tagcggtaag taggcttctg
    13261 gcgcagcagt tgcgaaatct cactgttagg atcgtccaga ttgccaaacg tcagcgcctt
    13321 cgtcgggcaa gcttcaacgc acgcgggaag cttaccggcc tgcaaattgg tcttacgaca
    13381 gaaatcgcat ttgtccgccg tcttcgtgac cggatggata aagcgcacgc ggtacggaca
    13441 ggcggcgatg cagtactgac aaccgacgca gagatcagga ttaacatcga caatcccgct
    13501 agcggcatca cgaaaagacg caccggtcgg gcagacgtca acgcacggca catgatcgca
    13561 gtgctggcaa gacttacgga agaagcgata tttcacatca ggaaattcgc cctgcggctc
    13621 gctacgaata atcgtcaggc gcgagacgcc ttccggcacc ttgttcactt cccgacaagc
    13681 atccatacag gcggtacagc cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac
    13741 cccattgata ttcaacgttt tcgccaccac gcgccctgcg gtgccgctga cggctgccag
    13801 cacgccgacg ccggtgagaa actggcgacg agaccaggtc atggctgctc cttaagcaac
    13861 ggaacagacg ccgggttaaa gttcggattg gtgcgctgat cgctgtggca atcgacgcaa
    13921 atcttaatcc gtcctttgtc acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa
    13981 tggcagctgg cgcacgccac tttggttacg tggacatcgt gcggccagaa cgctttttgc
    14041 aactgttccg gcagatgaca ggacatacag acgctgttct gctctccgac cttatacatc
    14101 ggctcgttaa agcgcatcac atctttcacc ccttcgcggt gttgcggtga tgcttggccg
    14161 tgacagttgg tgcaggtgac cggcaatttg ttattcgggt tgataacgga agcatgtttg
    14221 ccatgcatac cttcggtatc tggtttatga cagtccagac aggcggcatc cgggttacgc
    14281 tgctgggtaa cttcgtagcg atcaccagat gcctgcaccg caggggtagc ggtaattccg
    14341 ttcaggctcc acaacaggcc cgacgccagc accccagcag ttaataacga acgtaatacg
    14401 ctcatattca ctccattgtg ttttccgccc cttgcgaggc ggaacggggt tattggctta
    14461 acagaccgtt tttacgtgcc tgctcttccc actgcgggat caccgttttg atgaagtcct
    14521 gcttctcggc cttgatttgt tccatgttca ggccaatggc ctgctgggct ttctctttgg
    14581 tcgagatatc cgggatctgg atttcatggg tgataccttt ggtcgccagc aggcgcgcca
    14641 gtttggtgcg cgcatccgcc gctttatcca tcgccgtacc gagcatccgt agaccttctt
    14701 ccggtgcgtg catatgaata ccgtgggaag cgatcgccag atcccaacgc cactgggcat
    14761 gacggatatc gtcctgaatc ggcttcattt cggcttccgt cgcgcctgca tccagcgccg
    14821 ctttcgcttc gaagtgagcg tgaaccagtt gatcttcaac cttgattttc aggtcgttaa
    14881 tcgattgctt acgttccgcg accacttttt gcaaggcagc tttatcctgg gtatggcagt
    14941 tcgcacaagt ctgggcgaag ttatcaaacg gattaccaat tttatggtcg gtgtagagtt
    15001 tgccttcggc gttctgcact tttggcatat ggcagtcgat acaggtcacg ttgtttttac
    15061 cgtgaatgcc cgctgtccag gtttcatatt ccgggtgctg cgctttcagc attggcgttt
    15121 tcgacaggga gttagtccag tcagagaagg caattttgtc gtaatactgc tccatatttt
    15181 cgactttcat gccgtcatcc cacgggaatt taaccgcttt gtttttgccg tcgaagtaat
    15241 actccacatg gcactgaccg caaaccatcg attgctggtc gaaacgtccg gctttctcaa
    15301 aaggtttacc aatggcttcc atcgcgcgag ccgcatacgg acgggaaagg gttaactccg
    15361 gtttgccttt ggcgaactct ggtgaggcgg tgttatggca gtcggcacaa cctaagttgt
    15421 tgacgatttc cgggccgccg cgcgcccatt taccgtggaa gtagccatct tcgccgtctt
    15481 tctggatcag acgcgccaca tccgggcttt tacaactcca gcacgccatc ggtagcggac
    15541 catcttcagc gtttttcggc gcaccggtac gcagggtttc acgcacatcg gtcacagcaa
    15601 aggcatgtcc acgaggcttg ttgtaatcgc gcgagaaggg ataccccgcc cacaggatca
    15661 ccagccgtgg atcttccgcc agggcgtcaa cacgctctga ctgttccgag gtggctttcc
    15721 aggagagata ttgatcgtga tgctgcgggg caaaggtttc attcttcgct tccacagtta
    15781 caggttttgc gggagctgcc gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa
    15841 tcaataagct gaagatacgg cgtgcgttta tttttatcct tgtcataggg gcttcatccg
    15901 aattgcgcca ttgttgcaat ggcggttttt attgtttttc acgacagtaa ccgcacctac
    15961 actgtcatga cattgctcac ccctatgtgt aacaaataac cacactgtga atgttgtctt
    16021 taatcaattg taagtgcatg tgaaatacca ctttagagtt agtcagtatc ttcctctttt
    16081 tcaacagcat gcataactgc atgttcctca aagaattaat caacttttgt tggtgacctt
    16141 caaaaattac cctgccgttt atttgcacca ttctactttt gcgtgatctg tcgcccaaat
    16201 actaaacaaa actgccaata cccctacatt taacgcttat gccacatatt attaacatcc
    16261 tacaaggaga acaaaagcat gagccaaatt cacaaacaca ccattcctgc caacatcgca
    16321 gaccgttgcc tgataaaccc tcagcagtac gaggcgatgt atcaacaatc tattaacgta
    16381 cctgatacct tctggggcga acagggaaaa attctcgact ggatcaaacc gtaccagaag
    16441 gtgaaaaata cctcctttgc ccccggtaat gtgtccatta aatggtacga ggacggcacg
    16501 ctgaatctgg cggcaaactg ccttgaccgc catctgcaag aaaacggcga tcgcaccgcc
    16561 atcatctggg aaggcgacga cgccagccag agcaaacata tcagctataa agagctgcac
    16621 cgcgacgtct gccgcttcgc caataccctg ctcgagctgg gcattaaaaa aggtgatgtg
    16681 gtggcgattt atatgccgat ggtgccggaa gccgcggttg cgatgctggc ctgcgcccgc
    16741 attggcgcga tgcattcggt gattttcggc ggcttctcgc cggaagccgt tgccgggcgc
    16801 attattgatt ccaactcacg actggtgatc acttccgacg aaggcgtgcg cgccgggcgt
    16861 agtattccgc tgaagaaaaa cgttgatgac gcactaaaaa acccgaacgt caccagcgta
    16921 gagcatgtgg tggtactgaa gcgtactggc ggaaacatcg actggcagga agggcgcgac
    16981 ctgtggtggc acgacctggt tgagcaagcc agcgatcagc accaggcgga agagatgaac
    17041 gccgaagatc cgctgtttat tctctatacc tccggttcta ccggaaaacc aaaaggcgta
    17101 ctgcacacta ccggcggtta tctggtgtac gcggcgctga cctttaaata tgtctttgat
    17161 tatcatccgg gtgatatcta ctggtgcacc gccgatgtgg gctgggttac cggacacagt
    17221 tatttgctgt acggcccgct ggcctgcggc gcgaccacgc tgatgtttga aggcgtaccg
    17281 aactggccga cgcctgcccg tatggcgcag gtggtggaca agcatcaggt caatattctc
    17341 tataccgcac ccacggcgat ccgcgcgctg atggcggaag gcgataaagc gattgaaggc
    17401 accgaccgtt cgtcgctacg cattctcggt tccgtgggcg agccaattaa cccggaagcg
    17461 tgggagtggt actggaaaaa aatcggcaac gagaaatgtc cggtggtcga tacctggtgg
    17521 cagaccgaaa ccggcggttt catgatcacc ccgctgcctg gcgctaccga gctgaaagcc
    17581 ggttcggcaa cacgtccgtt cttcggcgtg caaccggcgc tggtcgataa cgaaggtaac
    17641 ccgctggaag gggctaccga aggtagcctg gtgatcaccg actcctggcc tggtcaggcg
    17701 cgtacgctgt ttggcgatca cgaacgtttt gagcagacct atttttccac cttcaaaaat
    17761 atgtatttca gcggcgacgg cgcgcgtcgc gatgaagatg gctattactg gatcaccggg
    17821 cgtgtggacg acgtgctgaa cgtctccggt caccgtctgg ggacggcaga gatagagtcg
    17881 gcgctggtgg cgcatccgaa gattgccgaa gccgccgtag taggtattcc gcacaatatt
    17941 aaaggtcagg cgatctacgc ctatgtcacg cttaatcacg gggaggaacc gtcaccagaa
    18001 ctgtacgcag aagtccgcaa ctgggtgcgt aaagagattg gcccgctggc gacgccagac
    18061 gtgctgcact ggaccgactc cctgcctaaa acccgctccg gcaaaattat gcgccgtatt
    18121 ctgcgcaaaa ttgcggcggg cgataccagc aacctgggcg atacctcgac gcttgccgat
    18181 cctggcgtgg tcgagaagct gcttgaagag aagcaggcta tcgcgatgcc atcgtaaccc
    18241 acaattgccg gatgcgaatc ggtaacggtt tgtaggcctg ataagacgcg acagcgtcgc
    18301 atcaggcatt gattgccgga tgcggcgtaa acgccttatc cggcctacat tcggcaaggg
    18361 ttacccgagc gttaaccttc tcccataagg gagcgggaat taaaacaatc cctacattac
    18421 ctctggagaa tctgtgatga atggtacgat ttatcagcgg atagaagaca atgcgcattt
    18481 cagggagtta gtcgaaaaac ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc
    18541 agtttatatc ggctttattt tactgatcgc cttcgcgccc ggctggctgg gcacgccgct
    18601 gaatccgaac accagcgtca cacgcggtat tccaattggt gttggagtga ttgtgatctc
    18661 ctttgttctc accggtatct acatctggcg ggcgaacggc gaattcgacc gtcttaataa
    18721 tgaagtcctg catgaggtac aagcatcatg aaaagagttc tgacggcgct tgccgccaca
    18781 ctccctttcg cagctaacgc cgcggatgct attagcgggg ccgtagagcg ccagccaacg
    18841 aactggcagg cgattattat gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac
    18901 tgggcatcaa aacgcgtacg ttctcgtagc gactactaca ccgcaggcgg caatatcact
    18961 ggcttccaga acgggctggc gattgccggg gactatatgt ccgccgcctc attcttgggg
    19021 atctccgcgc tggtgtttac ctccggctat gacgggctga tctactcgtt gggcttcctg
    19081 gtgggctggc caatcattct gtttctgatt gccgaacgtc tgcgtaacct gggacgctac
    19141 acctctgccg atgtggcctc ttatcgtctg aaacaagggc cgattcgtat tctttcggcc
    19201 tgtggttctc tggtagtggt ggcgctttac cttatcgctc agatggtagg cgcaggtaaa
    19261 ctgatcgagc tgctgtttgg ccttaactat cacattgcgg tggtgctggt cggcgtgctg
    19321 atgatgatgt acgtcctgtt cggcggcatg ctggcgacca cctgggtaca aattatcaaa
    19381 gctgtgctgt tgctgttcgg tgccagcttt atggccttta tggtgatgaa acacgtcggc
    19441 tttagcttca acaatctgtt cagcgaagcg atggcggtac acccgaaagg tgtcgacatc
    19501 atgaaaccgg gcggactggt gaaagatccg atctccgcgc tctctctggg gctgggactg
    19561 atgtttggta cggcgggctt gccgcacatt ctgatgcgct tctttacagt cagcgatgcc
    19621 cgcgaagcac gtaagagcgt gttctacgcc accggattta tgggctactt ctatattctg
    19681 acctttatta tcggcttcgg cgcgatcatg cttgttggtg cgaatccgga atataaagac
    19741 gcggcgggcc atctgattgg tggtaacaac atggcggccg ttcacctggc gaatgcagtg
    19801 ggcggcaacc tattcctcgg ttttatttca gcggttgctt tcgccactat cctcgcggtg
    19861 gttgcggatc tgacactggc gggcgcatcg gcggtttcgc atgacttgta cgctaacgtc
    19921 ttcaaaaaag gcgcgaccga acgtgaagag ctgcgggtat caaaaatcac cgtactgatc
    19981 ctcggcgtga ttgcgattat cctcggcgtg ctgtttgaga atcagaacat cgcctttatg
    20041 gtaggccttg cgtttgccat cgcggcgagc tgtaacttcc cgatcattct gctttccatg
    20101 tactggtcga aactgactac ccgtggcgcg atgctgggcg gctggctggg gctgattacc
    20161 gcagtggtgc tgatgatcct cggcccgact atttgggtac agatcctcgg tcacgaaaaa
    20221 gccatcttcc cgtatgaata cccggcgctg ttctctatca gcgtggcatt cctcggcatc
    20281 tggttcttct cggcaaccga taactcagcg gaaggggcgc gcgagcgtga actgttccgc
    20341 gcgcagttta tccgctccca gaccggcttt ggcgttgagc aaggccgcgc acattaatct
    20401 ctccccttcc ccggtcgcct gaccggggaa tacgcttcct ctccaacatg catcaccttt
    20461 tcccataata ttaaacaaat aaactcatta aaaaatgagc gatttttgac agtcgtagaa
    20521 aatgataatg cagagaatat gccttttctt tcttgttaat tataaggata ttttatgcgc
    20581 tataatggtt taaataatat gtttttccct ctttgccaga ttaacgataa ccactctatc
    20641 acaagttcat cacatacaaa gaaaacaaaa tcagataatt acagcaaaca tcataaaaac
    20701 acgttaattg acaataaagc cctctctctt ttcaaaatag atgatcatga aaaagtgata
    20761 ggcttgattc agaaaatgaa aagaatttat gatagtttac catcaggaaa aatcacgaaa
    20821 gaaacggaca ggaaaataca taaatatttt atagatatag cgttatatgc aaataataaa
    20881 tgtgacgata gaattacgag aagagtttac cttagtaaag aaaaggatgt atccattaag
    20941 gtggtatatt ttataaataa tgtcgccgtc cataataata ctatcgaaat tccacagata
    21001 gtaaatggtg gttacgattt ttcacacctt agcctgaagg gtatcgtgat taaagatgaa
    21061 gatttatcca attcgaattt tgcaggttgc agactacaaa acgctatttt ccaggactgt
    21121 aatatgtata aaacgaattt ttattacgcc ataatggaaa aaatactttt tgataattgt
    21181 attctcgatg actcaaattt cgctcagata aaaatggccg acggaactct aaatgcatgc
    21241 tccgctatgc atgttcaatt ctacaatgca gcaatgaata gagccaatat taaaaatacc
    21301 ttccttgact attcaaattt ttatatggcg tacatggctg aggtaaatct ttataaagta
    21361 atagcgccat atgttaattt atttaaagcc gaccttagtt tctctaaact cgatttaatt
    21421 aactttgaac atgctgatct gtctcgcgtc aatctgaaca aagcaatcct ccagaatata
    21481 aacttaattg atagcaaact cttttgtacg tggctgacaa atacattcct cgaaatggtt
    21541 atatgtaccg gctctaatat ggctaatgtt aattttaata atgccaattt aagcaactgc
    21601 catttcaact gttctatttt aacaaaagcc tgtatgttta atacccgtct ctatcgggtt
    21661 aatttcgatg aggctagcgt ccagggaatg ggcatttcca ttctccgtgg ggaggaaaat
    21721 atccccattg atagtgatac cctggtaaca ctacagaaat tctttgaaga agattgtacc
    21781 tctcatactg gcatgtcaca aactgaggat aatattaatg cagtcgctat gaagattact
    21841 gcagatatta tgcaacacgc agattgaggt taagtcgaag aacagccaat gaattgactt
    21901 tacaagagag ataacttatt tgttttagaa tcatgaatgt tcgcccgagc taacctgtta
    21961 aaggtgcaaa agattgcacc tttttcagat tacctcgggc ctggttacgt cgctttcggc
    22021 ttactgattt tcctcaatta gcaacgcttc aagcaaatcg agatcgtgca gtaatttttg
    22081 cagcgtttcg ttgctgatct cccgcgtggc gcgcaggtgg tacagttcag cacgttcaga
    22141 acgcaatgcc gccagacgga agcgacgctc aaggttctct tcctgcacgg aactttcaac
    22201 gtcgttacgt ccatcggcgc gacgacgcag gttaccaatg acgcgagaac tgacctccgt
    22261 aagcaactgg ttatcgatat tctcttcggt atccgccgcc agacgctcct ccattttctg
    22321 gatcgccaca atcgccactt ctgccgttgc cgctcgcgca atacgttctt ctttcaattg
    22381 ttgcgaatgg tccgcgactt caatgtgttg tagcagaata ggcaacatca ccacgccgac
    22441 aaacagcgag aagagaatga cgccagccgc caggaacacc agctcatagc gcgccgggaa
    22501 gacgttacca tccggcaaga gcagcgggat tgagagcaca ccggccagag tgatcgcccc
    22561 acgcacccca gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac caaactccat
    22621 cggcttcttc ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc agccgaaacg
    22681 gaccagcatc agcgccgcat atatcagaat aatatcggta aacagcatcc agatttcgac
    22741 gttagggtcg atttctgccg ccatcagcga cgtctccaga atacccggca gttgcagacc
    22801 taacagcagg aacaccatgc cgttaaagac aaattccagc atcgcccagg tgctgtttgc
    22861 gcgcaggcgc attgccagtg gcgcacggcg catcacaccg gagcgggtga tggtcatccc
    22921 ggcggcaacc gcagcgagga tgcccgaaac gccaatatgt tcggcaatca gataagaagc
    22981 gaatggcagc aagaacagca gaacgatctg cgtcgcgggt tcatcaccgc cccagcggct
    23041 gaggaatcgc agcgagcgac catacagcca gctcaccaca aaaccggcaa gaataccgcc
    23101 aatggctact ttcataaatt cgaccgtcgc accgccgaca gtgaagatca tcgtccccat
    23161 cgccactgcc acggcaaact tcaacgacac caggccggaa gcgtcattca tcaacgcttc
    23221 gccctgcaaa atgcccatga tttttttcgg gatgcgccct tcgccaacaa tcccggagag
    23281 cgccacagca tcggtcggag aaagtaccgc cgccagcgca aaggcgggga tcagcggaat
    23341 gcccggcacc acccagtaaa taaggaagcc aatgccgacc acggtgacca ccaccagcgc
    23401 cagcgcgagg ccgaaaatct ctcgaccatg ttcgagaaat tcacgggtcg gcgttttcca
    23461 gccatcagcg aacagcaacg gcgggataaa caagactaaa aagagttcag gatcaaactc
    23521 cacatgcaaa ccaaacgtcg gccacgccag tagcgcaccg atggcgattt gcataagcgg
    23581 aagcgggatc tgaaagggca tgacacgagt gaccaccccg gacagcgaga ccacgagggt
    23641 cattatcagt atggtgaaga agatttccat gcgttccctg tttgcgatgt tttttatgat
    23701 ctgagaaaga cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa
    23761 tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc cgatgaaaga
    23821 ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa cgaccgtacc
    23881 gatgttcaac ttgcgccatt caccggaaac cagacgacca atcaccagag tcgcgaagcc
    23941 gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc
    24001 caccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca gcatcagcag
    24061 gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag aaagcggaga
    24121 gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaacc cggttttacc
    24181 gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag
    24241 gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccgccgt cgatgatctg
    24301 cccatcttta tccagcaggt tcgcctggcc ggcgacagcg cggatagttc cggtggcatc
    24361 aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc
    24421 cataatgtcc aggctgccaa tcagtgaatt gccgttttca tcgctcaatg aaggcatggc
    24481 gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat
    24541 aatggtcagc agaatgccac cagggacttt cagtttttcc aagccgatga tcaccgccag
    24601 acctaccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc
    24661 atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca gcaggaacag
    24721 accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct
    24781 acggataccc gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca cggcacccag
    24841 cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa acgccgtcag
    24901 ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc
    24961 gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc
    25021 caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg ttaacccggc
    25081 aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga
    25141 accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc
    25201 gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt
    25261 gcggcaattt ggtgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg
    25321 gtaaaaaaaa gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg
    25381 cttttttctc ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat
    25441 cgtcgatgtg caagattgca gcgttatcga gccgtatacc gttctggatt gcgataaaaa
    25501 atcgtctgag atgacaaaaa tattgcggct aaaaagtata cttttacact ggcgaagaaa
    25561 cgtcggtgac atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat
    25621 gaggtgtgtt gacgtcgggg gaaaccctcc tgtgttccag cgggatagag agaaagacaa
    25681 agaccggaaa acaaactaaa gcgcccttgt ggcgctttag ttttgtgttt gcggataatt
    25741 ccaaaagagc tcgatcagtc ccctcgcccc ttcggggaga gggttagggt taggggaaca
    25801 ggcagcacca gtgcaaatat ttaccctcac cccggcccca tagttaacga tgagtcagct
    25861 tgtccttgtg gcgctttagt tttgttcatc ttccagcaag cgtgcgccgg taccttcttc
    25921 tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc
    25981 aatacatccg tccagttcgt cacgcagcgc cactaaggta tgaatgcgcc gatccaactc
    26041 ttctcgccat tgggacgaaa gctgtttcca ctctttcgca cttaacgtat gcccttctgg
    26101 caacacgcca aacgcttcac caatggtcgc cagcggaatg ccaatacgct gagcgatttt
    26161 gataattgcg acatatcgca acacatcacg tttatatcgc cgctgattgc cgctgttacg
    26221 gatactagta atcaaccctt tgctttcata gaaatgcagc gccgataccg ccacaccgct
    26281 gcgtttcgcc acttcgccag gggttagcag cgctttaatg cggggtaatt tcttttccat
    26341 aaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg aattaacgaa
    26401 ctgaacactg aaaagaggca gatttatgtc ccatcagaaa attattcagg atcttatcgc
    26461 atggattgac gtgcatattg accagccgct taacattgat gtcgtcgcaa aaaaatcagg
    26521 ctattcaaag tggtacttgc aacggatgtt ccgcacggtg acgcatcaga cgcttggcga
    26581 ttacattcgc caacgccgcc tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc
    26641 gatttttgat atcgcaatgg acctgggtta tgtctcgcag cagaccttct cccgcgtttt
    26701 ccgtcggcag tttgatcgca ctcccagcga ttatcgccac cgcctgtagt tttattgccc
    26761 gcgcgttaac tcccgcgcgg gtaattgctc catccattgc ataaacacct gcggcggcat
    26821 cgccttcgca aaaaaccatc cctggcaata gcgcacgccg cgtttgcgca gccagttaac
    26881 ctgctcctca gtttcgacgc cttcagcgat cgtttttaac cccaggctgt gcgccagctc
    26941 gatgatgtgt tccgcaatca aatgactggt tttgtgggtg gtcagcgttt caacaaacga
    27001 tttatcgatt ttcaaaatat cgacattcaa tgatttaagg ttatgcaagt tagagtagcc
    27061 aataccaaaa tcatcaattg ccacttcgta accagcctgg cggaaagcca gaattaatcg
    27121 gcgtcatttt ttcgacatca agaaatgcat gctcagtcac ttcaaattta atttgctgcg
    27181 gacgcaccgc gtattgctct gttttctgat tgattcgcgc tatcaaccgt gacgtatgaa
    27241 aatcggaggc cgacaggtta atagaaacat agcgatcggc atgtgttgcc aggtacgcgc
    27301 ccagatcgcg gaagacatta tcaataacat aatcagttat ctgttctatc atcccctcct
    27361 tttctgccag cggaataaac tctgccggat tcattatttg cccctgctca ccaggccaac
    27421 gtaacaacgc ttcagcgccg atacattttt ctgttttgat atcgattatt ggctggtaat
    27481 aaagacaaag ttgatgtttt tcgagggcgc gttgcaattt acgtttggga gataaatagt
    27541 tttgtcgaat acgtagccag agtaatagca gaacaagact ccccaaaata ccggcgggca
    27601 acgcaaaaat aagatgatta taaaaatggg ttataagacg ttgatatgaa gtcgcaacaa
    27661 tggctgcaat tgggcgtttt gtcgaataaa ttgtcgcata taaatagcca tttctttgta
    27721 cggttaaatc cttcaaatga atcagcggag aaaacgttgc tgccgaggcc tctttgctta
    27781 acgagaaaaa ggttttcgtc accgtatcat acacacccca ttgcaatgtc gggtcatcag
    27841 acatcacttc actccagaag agagggttga taaccgccac ataatttccc cgctgcatat
    27901 aggtcatttt atagccagag aaaaaaggcg tatcgcggta ataatagata gaaacgttag
    27961 gttcacgctt ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct atcagcgatg
    28021 agcataaaaa atggttatca cgaattcatt aatataaaga tagccacgaa taatattcaa
    28081 cattcgcttt tgatgggctg gagtgcaaac ttgcccctga tagcgttcag ctgcatcgct
    28141 cactaaatct gcctggcgaa tgaccagctc ggatttatcc agtgccagtt gagcaaaggt
    28201 tcggagctga ttattcactt ctgattttgc ccaaaggaag gcaatccata gcgaaagaat
    28261 gatgggaaag agaactaaaa agataatgcc cggcaacgcc agtaattggt gtcgtgcacg
    28321 atgactcatt gctatcaatt tcctgcctgt ttatacccag ccattgcaag gtgaaaccct
    28381 ggctaccgac gtctttagat aaatcttacg gtataaatag cacttttgcc tgatttgaaa
    28441 ttgatacctt tttaatcgtc acctggatga aaataattca ttgatattca aaattaattt
    28501 tctacaggaa tacagaggcg gcgggaagat atgcccttaa tgccactgaa aaaccacata
    28561 atttgtacag caataaaagt cacggcctaa tatggctaac aaacaatcaa ttgtgatata
    28621 gttcacaaaa ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga
    28681 tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt
    28741 ttcagaggaa ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcaac
    28801 ttcttagtgc cgtaatgatg tttctggcca gcacactcaa catccgtttt cgtcggtctg
    28861 attatgtcgg gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc tgttggttcg
    28921 caatggggtt gcttggcatc acaatggcgg acatcaccgc tatctggcac aacatcgagt
    28981 cggtgatgat agaagagatg aatcagacac cgccacaatg gccaatgatt ttgacctgat
    29041 acgtagaa
//
LOCUS       DAJVRL010000050        28429 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVRL010000050 DAJVRL010000000
VERSION     DAJVRL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.050"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            562..1332
                     /locus_tag="OQN02_002139"
     CDS             562..1332
                     /locus_tag="OQN02_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1642924.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1323..1796
                     /locus_tag="OQN02_002140"
     CDS             1323..1796
                     /locus_tag="OQN02_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1642925.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1903..2442
                     /gene="dnaT"
                     /locus_tag="OQN02_002141"
     CDS             1903..2442
                     /gene="dnaT"
                     /locus_tag="OQN02_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1642926.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2445..3182
                     /gene="dnaC"
                     /locus_tag="OQN02_002142"
     CDS             2445..3182
                     /gene="dnaC"
                     /locus_tag="OQN02_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1642927.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3231..3725
                     /locus_tag="OQN02_002143"
     CDS             3231..3725
                     /locus_tag="OQN02_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1642928.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3979..6270
                     /gene="opgB"
                     /locus_tag="OQN02_002144"
     CDS             3979..6270
                     /gene="opgB"
                     /locus_tag="OQN02_002144"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1642929.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6408..7430)
                     /gene="lgoD"
                     /locus_tag="OQN02_002145"
                     /pseudo
     CDS             complement(6408..7430)
                     /gene="lgoD"
                     /locus_tag="OQN02_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7569..8483
                     /locus_tag="OQN02_002146"
     CDS             7569..8483
                     /locus_tag="OQN02_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1642930.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8698..10059
                     /locus_tag="OQN02_002147"
     CDS             8698..10059
                     /locus_tag="OQN02_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1642931.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10108..11772)
                     /gene="tsr"
                     /locus_tag="OQN02_002148"
     CDS             complement(10108..11772)
                     /gene="tsr"
                     /locus_tag="OQN02_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1642932.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11891..12337)
                     /gene="hpaR"
                     /locus_tag="OQN02_002149"
     CDS             complement(11891..12337)
                     /gene="hpaR"
                     /locus_tag="OQN02_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1642933.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12609..13898
                     /locus_tag="OQN02_002150"
     CDS             12609..13898
                     /locus_tag="OQN02_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1642934.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13895..15361
                     /gene="hpaE"
                     /locus_tag="OQN02_002151"
     CDS             13895..15361
                     /gene="hpaE"
                     /locus_tag="OQN02_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1642935.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15363..16214
                     /gene="hpaD"
                     /locus_tag="OQN02_002152"
     CDS             15363..16214
                     /gene="hpaD"
                     /locus_tag="OQN02_002152"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1642936.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16224..16604
                     /locus_tag="OQN02_002153"
     CDS             16224..16604
                     /locus_tag="OQN02_002153"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1642937.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16672..17475
                     /gene="hpaH"
                     /locus_tag="OQN02_002154"
     CDS             16672..17475
                     /gene="hpaH"
                     /locus_tag="OQN02_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1642938.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17486..18289
                     /gene="hpaI"
                     /locus_tag="OQN02_002155"
     CDS             17486..18289
                     /gene="hpaI"
                     /locus_tag="OQN02_002155"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1642939.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18311..19666
                     /gene="hpaX"
                     /locus_tag="OQN02_002156"
     CDS             18311..19666
                     /gene="hpaX"
                     /locus_tag="OQN02_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1642940.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19676..20566
                     /gene="hpaA"
                     /locus_tag="OQN02_002157"
     CDS             19676..20566
                     /gene="hpaA"
                     /locus_tag="OQN02_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS1642941.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20593..21530
                     /locus_tag="OQN02_002158"
                     /pseudo
     CDS             20593..21530
                     /locus_tag="OQN02_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21757..23315
                     /gene="hpaB"
                     /locus_tag="OQN02_002159"
                     /pseudo
     CDS             21757..23315
                     /gene="hpaB"
                     /locus_tag="OQN02_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23333..23845
                     /locus_tag="OQN02_002160"
     CDS             23333..23845
                     /locus_tag="OQN02_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1642942.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24238..26388
                     /gene="btsT"
                     /locus_tag="OQN02_002161"
     CDS             24238..26388
                     /gene="btsT"
                     /locus_tag="OQN02_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1642943.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26438..26641
                     /locus_tag="OQN02_002162"
     CDS             26438..26641
                     /locus_tag="OQN02_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1642944.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26652..27608
                     /gene="yjiA"
                     /locus_tag="OQN02_002163"
     CDS             26652..27608
                     /gene="yjiA"
                     /locus_tag="OQN02_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1642945.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27677..>28429)
                     /locus_tag="OQN02_002164"
     CDS             complement(27677..>28429)
                     /locus_tag="OQN02_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCS1642946.1"
                     /translation="TKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTED
                     FNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHA
                     DNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQAL
                     VNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYL
                     TKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6959 a         7431 c         7127 g         6912 t
ORIGIN      
        1 taaatgtttc cagcaaatta atagattaag cgaataaatt atccttagca ttcactttca
       61 tatcagcata ctcctgctgc acaacaactc cactgcatgt gtcagaaggt tagcatcaaa
      121 tgataacctg gtgaaatagc ctggagacat ttaaaatatc tacattaagt tgttagaaat
      181 acttaatgaa aaacagaaat ctcattcatt taatttataa ttaaatgata tctcttcccg
      241 cagaatgcgg gaatcacaca atcaatattt attcaataaa atctgatgat tttaacttgt
      301 taacactaaa aaacgcgccc agctatctat tatcctgacc cgtaaaggct aaacaataaa
      361 ttcattatag tgataatatt catgtgatag tttgtttgcg gcgagagata attcgctttt
      421 tatcaccgag cttgtcccaa taaccaggat taatcagcgc aattttgatc ttaacctacc
      481 gcaaaacaac cgtgcttcat gatacaaatg gattctcatt atctgaacaa tacgcagcac
      541 gtctatgaca aagggcgagt tatgcaaact gagcaacagc gagccgtaac gcggctttgt
      601 atccagtgtg gattatttct tttgcaacat ggtgcggaaa gcgcgttggt tgatgagctt
      661 tcctcacgac tgggtcgggc gctgggaatg gacagcgtcg aaagttctat ctcttcgaac
      721 gccatagtgc tgacaactat taaagatggg caatgcctga catcgacacg taaaaatcac
      781 gatcgcggca ttaatatgca tgtggtgact gaagtccagc acattgtgat tcttgcggag
      841 catcatctgc tggattacaa aggcgtagag aaacgattta gccaaattca accattacgt
      901 tacccaagat ggctggtagc cttaatggtt ggcctttctt gcgcctgttt ctgtaaactc
      961 aataacggtg gctgggatgg tgccgtcatc accttctttg ccagtacgac cgcgatgtat
     1021 atccgccagc tgctggcaca acgtcatctt catccacaga tcaacttttg ccttaccgct
     1081 ttcgccgcca ccaccatttc cggattgctt ttgcaactcc ccactttcag caataccccc
     1141 accattgcga tggccgccag cgttctgctg ctagtgccgg gctttccgtt gattaatgcc
     1201 gtcgccgata tgtttaaagg ccacatcaat accggactgg cacgctgggc gatcgccagt
     1261 ctgctgacac tggctacctg cgtcggcgta gtgatggcac tgacgatttg ggggctacgc
     1321 ggatgggtgt gatcgaattt ctgttagcgt tggcgcagga tatgatcctc gccgccattc
     1381 ctgcggtcgg ctttgcgatg gtgttcaacg ttcccgtgcg ggcgttacgc tggtgtgcgc
     1441 tgcttggctc gataggtcat ggttcccgaa tgatcttgat gaccagcggg ttgaatattg
     1501 agtggtcaac ctttatggct tctatgctgg tcggtaccat tggtattcaa tggtcgcgct
     1561 ggtatctggc gcatccgaaa gtgtttaccg tggcggccgt tatccctatg ttcccgggca
     1621 tatcggctta taccgcaatg atttcgtcgg taaaaatcag ccagttaggt tacagcgaac
     1681 cgttgatgat taccctgtta accaactttc ttacagcttc atcgattgtt ggtgcgttat
     1741 ccatcggtct ttccattcct agattatggt tgtaccgcaa gcgccctcgc gtataaaatt
     1801 gcctcgctta agcacacgga tgagagacag cctcctctcc tccgtgtgtt actataaaag
     1861 ttatctccct tctcgttcat cgttccatat ttgagaaaca gtatgtcttc cagagttttg
     1921 accccggacg tcgttggtat tgacgccctg gtacacgatc accaaaccgt tctggcaaaa
     1981 gctgaaggcg gtgtggttgc cgtatttgct aacaatgccc cggcgtttta tgccgtcacg
     2041 cctgcacgcc tggctgaact gctggcgctg gaagaaaagc tagcgcgtcc gggaagcgat
     2101 gtcgctctgg acgaccaact ctatcaggaa ccgcaagccg ctcccgttgc tgtacccatg
     2161 gggaaattcg ccatgtatcc ggactggcaa cccgatgccg attttatccg cctggcggcg
     2221 ctatggggcg tggcgctaag agagccggtg accaccgaag aactggcctc attcattgcc
     2281 tactggcagg cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa actggcgcgc
     2341 agcctgcaaa tcggtcgtgc cagcaacggc ggactgccga aacgagatgt gaatacggtc
     2401 agtgaacctg acagccaaat tccaccagga ttcagagggt aacgatgaaa aacgttggcg
     2461 acctgatgca acgcctgcaa aaaatgatgc ctgcccatat caaacctgca ttcaaaacgg
     2521 gtgaagagct actggcctgg cagaaagaac aaggggcgat ccgctccgcc gctctcgaac
     2581 gtgaaaatcg ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt cgtccactgc
     2641 atcagaactg ctcctttgag aactatcgcg ttgagtgtga agggcagatg aatgcgttaa
     2701 gcaaagcgcg ccagtatgtc gaagagtttg acggcaacat cgccagcttc atcttttctg
     2761 gtaagccagg aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag ctgctgctac
     2821 gcggtaaatc cgtattgatc atcaccgtgg ccgatattat gtcggcgatg aaagatacct
     2881 tcaggaatag cggtaccagc gaagaacaac tgcttaacga tctgagcaac gttgatctgc
     2941 tggtgatcga tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg atcatcaacc
     3001 agatcgtcga tcgccgctct tcttccaaac gcccaaccgg gatgctgacc aacagtaata
     3061 tggaagagat gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc ctgggtaaca
     3121 gtttatgggt gatcttcaac tgggatagct accgtagccg ggtaacgggt aaagagtatt
     3181 aatccccgtt tctggcggta tactgcgaca caattccacc ttgagtaatg atgatgaaaa
     3241 ctattaaaca tcttctgtgc tgtgccattg ccgccagcgc attaatttcc accggggtgc
     3301 atgctgcgtc ctggaaagat gcgctctcca gcgccgccag cgaacttggc aaccaaaaca
     3361 gcacgacaca ggaaggcggt tggtcactcg cgtcattaac taacttgctt agcagcggaa
     3421 accaagcctt aagcgcagat aacatgaaca atgccgcagg cattctgcaa tactgcgcga
     3481 agcaaaagct ggcttcggta accgatgccg aaaacatcaa gaaccaggtg ctggaaaagc
     3541 tgggcctgaa cagtgaagag caaaaagaag acaccaacta tctggacggt attcagggtt
     3601 tgctgaaaac aaaagatggt cagcaactca atctggataa catcggaacg actccgctgg
     3661 cagaaaaggt gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg aacttcattt
     3721 cctgaaccgc gaaacgccgc gttttagctg ttacccggct gacgcggctc tctcctgccc
     3781 ttttccctgc tcctggacgg ttttccccct gttacacggg aagcgaccgc gatcaaaaaa
     3841 gtctgtttat aaactaattc ctaacaacat cgcatttttt tgacactaga attgcagcaa
     3901 tatggcgacg ccattacatt gtgcttctca aaaagcttaa acgttggcta aacggcccgg
     3961 tttagtgagg atcgcccatt gtcagaacta ctctctttcg ccctttttct cgcctctgtg
     4021 ctgatttacg catggaaagc gggacgtaac acctggtggt ttgcagccac gttaacggtg
     4081 ctggggctat ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt tactggcgat
     4141 ggtattaacg acgcggttct ctatacctta accaacagcc tgaccggtgc tggcgtcagc
     4201 aaatacatcc tgccgggtat cggcattgtg ctggggctga cggcggtgtt cggtgcgctg
     4261 ggctggatcc tgcgccgtcg tcgtcatcat ccgcaccatt ttggttacag cctgctggcg
     4321 ctcttactgg cgctgggttc ggtggacgcc agcccggcat ttcgtcagat aacggaactg
     4381 gtgaaatccc agtcacgcga tggcgacccg gactttgcgg cttattataa agagccgtcg
     4441 aaaactatcc ctgacccgaa actcaacctg gtttatatct acggcgaaag tctcgagcgg
     4501 acctattttg ataacgaagc tttcccggat ctcacgcctg aactgggcgc gttgaaaaat
     4561 gaaggcctgg atttcagcca cacgcagcag ctgccaggaa cggattacac gattgcgggc
     4621 atggtggctt ctcagtgcgg cataccgctg ttcgccccct ttgaaggcaa cgcctccgcc
     4681 tctgtctcca gcttcttccc gcagaacatc tgtctgggcg atatcctgaa aaactcgggt
     4741 tatcagaact atttcgtgca gggcgcaaat ctgcgttttg ccggtaaaga tgtgttcctg
     4801 aaatcgcacg gcttcgacca cttatacggc tcagaagagc tgaaaagcgt ggtagccgac
     4861 ccgcactatc gcaacgactg gggattctac gacgataccg ttctcgatga agcgtggaaa
     4921 aagtttgaag agctttcccg ctcaggtcag cgattctcac tgtttaccct gacagtcgat
     4981 acccatcacc cggatggttt tatctcgcgc acctgtaacc gcaaaaaata tgattttgac
     5041 ggtaagccga atcagtcatt cagcgcggta agttgcagcc aggagaatat cgcgacgttt
     5101 atcaacaaaa tcaaagcgtc accgtggttt aaagatactg tcatcgtcgt ctcttctgac
     5161 catttagcga tgaacaacac ggcgtggaaa tacctcaata agcaggatcg caataacctg
     5221 ttttttgtca ttcgtggcga caagccgcag caaaagacgc tggcagtgaa gcgtaacacg
     5281 atggataacg gcgcgacggt gctggacatt ctcggtggcg ataactatct cggacttggt
     5341 cgtagcagtt tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa agagaaaaca
     5401 ttggcgtgga agccagatat catccgcctg tggaaattcc ctaaagagat gaaagagttc
     5461 accatcgacc agcagaaaaa catgattgcc ttctcgggta gccatttccg tttgccgctg
     5521 ctgttgcggg tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata ctcagcaccg
     5581 ctgcgtttcc agctggccga tttcgctcca cgcgacaatt tcgtctgggt tgacagttgc
     5641 tacaagatgg cacaactctg ggctccggaa ctggcacttt ccaccgactg gtgtgtctcg
     5701 caggggcagc ttggcggtca gcaaattgtt cagcatgttg acaaaacaac atggaagagc
     5761 aagacggcat ttaaagatac ggtcatcgac atggcgcgtt acaaaggcaa tgtcgatacg
     5821 ctgaagattg ttgataacga tattcgctac aaagccgaca gtttcatctt taacgtcgcc
     5881 ggtgcgccag aagaggtgaa acagtttagc gggatttccc gtccggagtc gtggggccgc
     5941 tggtccaacg cgcagctggg cgatgaagta aaaatcgagt acaagcatcc gctgccgaag
     6001 aaatttgacc tggtgattac cgccaaagca tacggcaata acgccagccg tcctattccg
     6061 gtacgcgtag gcaatgaaga acaaactctt gtgctgggca atgaagtgac caccaccaca
     6121 ctgcatttcg ataacccaac cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc
     6181 tcaaccaacg aagggaatat cctcggacac tcgccgcgta agctcgggat cggcatggtg
     6241 gaaattaaag tggtagaacg tgaagggtaa ttcctgactg atgtgtaggt cgcatagggt
     6301 gcgacctaca cgccttctcc ggcttacaac acattattaa gaaacaaaaa aaacagcgcc
     6361 caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca gaaagtaatt
     6421 acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc tgccagcgtg
     6481 gcgaacggat agcgatgggt taacatcatg tcggcagtga ttttcccttc cgccatcagt
     6541 cgaccgactt tagcgaaatc ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct
     6601 tttttatgga attccggatc ggagaactgc aactcacctt taaacagacc gacaaatacc
     6661 accgtgccgc cgtgacgaat cagattcacg gtattattca tcgcatgttg attacctgtc
     6721 gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgcagctg cgcgtcaaaa
     6781 tcttcggcta acgggtccag cacgggcaac tccagacgcg ttgccacatg ttcacggcgc
     6841 gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat tgccgccgcg
     6901 cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc aatggctgcg
     6961 cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgcggccgc ctgcgggtca
     7021 ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact aaaaccgcca
     7081 tcctgatgca cgccaatgac tgaaattttt tcacagcagt tggtacgccc gcttttacac
     7141 gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc atttttaaga
     7201 tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc taaaacccgt
     7261 ggataactaa aaaatggttg atttccaccc taggcatgaa tatcggtacc gcaaatcccg
     7321 acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc gcgttgtttc
     7381 cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat gattcggcta
     7441 tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga acggaaaatc
     7501 tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg actcacaagg
     7561 aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag gtcattgatg
     7621 atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg gcggaaatgt
     7681 acaacatcag ccgcactaca gtgcgtcata ttctcagcca attacgcgaa tgcggcgtct
     7741 taacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccatgat gacggtttcg
     7801 cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct tttttcacga
     7861 tgataaacca gcgtcagtta cgccctaggg aaactttttc tgaactgcaa ctggctcggg
     7921 ccgcgggtgt cagcccggtg gttgtaagag aatatctttt aaaattcggc cgttataatc
     7981 ttattcaaag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa tcctacgccg
     8041 agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac tttctcaacc
     8101 tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctagaacgt catcggctac
     8161 tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga gattttcact
     8221 ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag atcatctcag
     8281 tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc aatatcattg
     8341 ccgtcgatga acatatgacc attctcagcg ctctgatttg ccgcagtgac cttgatgcca
     8401 cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc aatgattcga tccattaatg
     8461 agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta gcgggtgcac
     8521 ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac aatcagtcga
     8581 ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc gttaatgtca
     8641 accttgcgta atgcacaatt acgtaaacaa ttaaaattat aaatacagga gtccggcgtg
     8701 gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc gtcggcagat
     8761 attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg cattcaaacc
     8821 accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg cagttcgctg
     8881 tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga aatcggcgct
     8941 ttgctctccg tgttttcact cgcttacggc attgcgcaac ttccttgcgg cccactattg
     9001 gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc actgttccag
     9061 gccatgtctg gcatggtgca cagctttacg cagttcgtgt tggtgcgtat cggtatgggg
     9121 attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg gttcaacatc
     9181 aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg cgttgctgta
     9241 agcccaccga ttctggcggc aatgatgctg gtgatgggct ggcgcgggat gtttattacc
     9301 attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg caaccgcgag
     9361 cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt caatgcccgc
     9421 cgagatccgc tcaattttgc cgaatggcgc agcctgttcc gtaaccgcac aatgtgggga
     9481 atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc ctggcttcct
     9541 ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat ggcggctatc
     9601 cctttcctgt ttggggctgc cgggatgctg gtcaacggtt acgttactga ctggctggtc
     9661 aaagggggaa tggctccgat taaaagccgt aagatctgca ttattaccgg gatgttctgt
     9721 tctgccgcct ttacgctgat agtaccacaa gcgacaacat ccatgatggc ggttctgctg
     9781 attggcatgg cactgttctg tattcacttt gccggaacat cctgctgggg cttgatccac
     9841 gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt tgccagcttc
     9901 atctgcgcct ctttcgcgcc gatcattact ggttttattg ttgatacaac ccactcattc
     9961 cgtctggcac taatcatttg cggttgcgtc accgcagcgg gagcactggc gtacatcttc
    10021 ctggttcgcc agccgatcaa cgacccgcgt aaagattaaa ctccctatgc ccgataacat
    10081 tttgcttatc gggcattttt atggcgatta aaacgtttcc cagttctccc cgctatctgc
    10141 cactgccatt ttacgcggcg tagctggcgt cacggttttt accacagccg atgtttcacg
    10201 ctgctgttgc tgctgttgct gaatccggaa cactgccaca gcttcggtca ggcgactggc
    10261 ctgctcttcc agcgcggcgg cggcagcggc agattcttcc accagcgcgg cgttctgttg
    10321 agttacccgg tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc
    10381 atcagaagca gaagctattt cgcccataat gtccgtcacg cgggtcacag cactgacaat
    10441 ctccgccatt gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac
    10501 cgagtcttca atcaggcttt taatttcacg aaccgcctgg gcgctacgct gggccagatt
    10561 acgcacttct cccgcaacca ccgcaaaacc acgtccttgc tcacccgcac gcgctgcttc
    10621 aaccgccgcg ttcaaagcca gaatattggt ctggaaggta atgccgtcaa ttacgctgat
    10681 aatatcggcg attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc
    10741 taccactttg ccgccgcgtt gcgccgtttc agaagcactt aatgccagat ggctggcctg
    10801 gcgcgcattc tcggcgttct gtttcaccgt tgcggtcagt tgctccatgc tggctgccgt
    10861 ctcttccagc gaagcggcct gttgctcggt gcgcgaagag agatcattat tgccggtagc
    10921 gatttcgctg gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg
    10981 catcagctct ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc
    11041 atccacctca atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag
    11101 gcgattcatt ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc
    11161 cagtacgacg atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt
    11221 atcgctgacg gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt
    11281 ctcgaaaccg tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc
    11341 acctaacagt tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat
    11401 ctctgccgct gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca
    11461 gtttttttcc gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga
    11521 accgctacca atattattct gatccatcat gtagcggata cccgcgcggt tgagggtgtt
    11581 acgcgtctgc aacaacgcga cccagctgcc attcagcgtg ggttgctgct ggcgaatggt
    11641 ttgtaaaaca gtgaaatttt ctttgtcatt ctttaaggca ttaaaaaaca gaccgcctga
    11701 tgtcagttgt aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat
    11761 acgttttaac atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca
    11821 aaaactttat gaaaaggtgt aacccgccat ccatggaaaa accaggcgct ggttatcaga
    11881 tactaaaaag ttattcatta tcgcccggga tgtcttcttg tcgggaattg cccagagcaa
    11941 taaattcctc taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa
    12001 tctgccgata ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt
    12061 tggtcagcga gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac
    12121 cgtcgcgttc catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat
    12181 acgccagatc gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact
    12241 gttgctcagt caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc
    12301 gcgcctggag caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa
    12361 aatcattaat atagaaacaa ttaattatca cgttaccggg actgagctgc ccccgcaatt
    12421 cttcatttta tcaaaattga tgatatttta ttaacttact gatatttaat attttattat
    12481 ctttaaatgt aaatagtttg ttaattagat cacatttata tcacttactt ttgccaaact
    12541 acgcaatcgg gaataaaagt aatcattaac atattaatga ttaagatgat cgaatccgag
    12601 gagtggtaat gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc caacttgatg
    12661 cctggcagga agcgttccag caatctccct acaaagcccc gcctaaaact gcggtctggt
    12721 ttattaaacc gcgcaatacg gtgattggtt gcggtgaacc gattcccttt ccacagggtg
    12781 aaaaagtact gagcggtgcg actgttgcgc tgattgtggg gaaaacggcg acgaaagtac
    12841 gtgaagaaga tgcggcagag tacatcgccg gatatgcgct ggctaacgac gtcagcctgc
    12901 cggaagagag cttttaccgt ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca
    12961 ttggcgaaac cgtggctctc agcaatgtcg ataatctgac tatctatacc gagatcaacg
    13021 ggcgtcctgc cgatcactgg aataccgccg atttacaacg taacgccgca cagctgctga
    13081 gcgccctgag cgaatttgcc acgctgaatc caggcgatgc tattctgctc ggcacgccac
    13141 aggcgcgcgt ggaaatacag ccaagcgatc gcgtgcgtgt tctcgcagaa ggtttcccgc
    13201 cgctggaaaa tccggtagtg gacgaacgtg aagtgaccac gcgcaagagc ttcccaacgc
    13261 tgccacaccc gcacggtacg ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg
    13321 aactggaatt taagccaccg gaagagccgc tggtgttcct gaaagcgccg aataccctca
    13381 ctggcgacaa ccagacctcc gtgcgcccga acaatattga atacatgcac tacgaagcgg
    13441 agctggtggt agttattggc aagcaggcgc gtaacgtcag cgaagccgat gccatggatt
    13501 atgtcgcggg ctacaccgtg tgtaacgact acgccattcg cgactatctg gaaaactact
    13561 accgccctaa cctgcgggtc aaaagccgcg acggactgac gccgatgctt tcaaccatcg
    13621 tgccgaaaga ggcgatcccg gccccgcata atctgaccct tcgcaccttc gtcaacggcg
    13681 agttacgcca gcaaggcacc accgccgatc tgatcttcag cgtgcccttc ctgatcgcct
    13741 acttaagcga atttatgacc ctgaatccgg gcgacatgat cgccaccggc acaccaaaag
    13801 gcttatctga cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc
    13861 tggtgaaccg aattgtgagt gaggaaacag cgaaatgaaa aaagtaaatc attggatcaa
    13921 cggcaaaaat gttgcaggta acgactactt ccagactacc aatccggcaa cgggtgaagt
    13981 gctggcggat gtggcctctg gcggtgaagc ggagatcaat caggcggtag cggcagcgaa
    14041 agaggcgttc ccgaaatggg ctgatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg
    14101 tctgggcgat ctgatcgacc agaacgtgtc tgagatcgcc gcgatggaaa ccgcggacac
    14161 gggcctgccg atccatcaga ccaaaaatgt gttgatccca cgcgcttctc acaactttga
    14221 atttttcgcg gaagtctgcc agcagatgaa cggcaagacc tatccggttg acgacaagat
    14281 gctcaactac acgctggtgc aaccggtagg cgtttgtgcg ctggcgtcgc cgtggaacgt
    14341 gccgtttatg accgccacct ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt
    14401 gctgaagatg tcggaactct ccccgctgac cgccgaccgc ctgggtgagc tggcgctgga
    14461 agctggtatt ccggcgggcg tgctaaacgt ggtacagggc tacggcgcaa ccgcaggcga
    14521 tgcgctggtc cgtcatcatg acgtgcgtgc cgtgtcgttc accggcggta ccgccaccgg
    14581 gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc atggagctgg gcggtaaatc
    14641 accggtgctg atttttgaag atgccgatat tgagcgtgcg ctggacgccg ccctgttcac
    14701 catcttctca atcaacggcg aacgctgcac cgccggttcg cgcatcttta ttcagcagag
    14761 catctatcca gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga
    14821 tccgaacgat ccgaataccc aggttagggc gcttatcagt cagcaacact gggaaaaagt
    14881 ctccggctat atccgtctcg gcattgaaga aggcgcaacc ctgttggcgg gcggcccgga
    14941 taaaccgtcc gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc caacggtgct
    15001 ggcggacgta gataaccgta tgcgcgttgc ccaggaagag attttcgggc cggtcgcctg
    15061 cctgctgccg tttaaagacg aagccgaagg cttacgcctg gcaaacgacg tggagtacgg
    15121 cctcgcgtcg tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat
    15181 tgaagcaggc atggtgttcg tcaacaccca gaacgtgcgc gacctgcgcc agccatttgg
    15241 cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc
    15301 ggaaatgaag aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg
    15361 atatgggtaa gttagcgtta gccgccaaga tcacgcacgt accgtcaatg tatctctctg
    15421 aactaccagg aaaaaaccac ggttgtcgcc agggtgcgat cgacgggcat aaagagatca
    15481 gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt tttcgatact cactggctgg
    15541 tcaacagtgc ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg
    15601 agctgccgca ttttattcgc gacatgacct acaactacga gggcaacccg gagttggggc
    15661 agctaattgc cgaggaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aatattccca
    15721 gcctgaaact ggaatacggc acgctggtac cgatgcgcta catgaatgaa gacaagcact
    15781 tcaaagtggt ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc
    15841 tgggcgaagc gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca
    15901 gcggttcgtt atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct
    15961 acacccgcga gttcgaccgc cagatggacg agcgtgtggt gaagctgtgg cgcgaaggcc
    16021 agttcaaaga gttttgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca
    16081 atatgcacga cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg
    16141 tggagtttat taccgagctg ttcccaagct ctggcaccgg tcaggttaac gctgttttcc
    16201 cgctgcccgc gtaaggacgt tttatgccgc actttatcgt ggaatgtact gaaaatattc
    16261 gtgaagaagc ccgcttacct gagctgtttg ccagtgttaa cactgcactg gcggcaacgg
    16321 ggatttttcc gcttggggga atacgtagcc gggcgcactg gatcgatacc tggcaaatgg
    16381 ctgatggcaa gcacgattac gcctttgtgc atatgacgct aaaaatcggc agcggacgca
    16441 gtctggaaag ccgtcaggaa gtgggcgaaa tgctgtttga tctgattaaa acgcacttcg
    16501 cctcactgat ggagagccgt tacctagcgc tttcatttga gattgcagag ctgcacccga
    16561 cgttgaattt caaacagaac aacgtacacg cgttgtttaa ataaatttgc accagcttcc
    16621 aggccggatg ataaggcgct gccgcatccg gcaatgccac aggatatcgc tatgttcgat
    16681 aaacacaccc acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag
    16741 atccgcgcga tctcgctgga ttacccggag atcaccatcg aagacgccta cgccgtgcag
    16801 cgcgaatggg ttcgcctgaa aatcgccgaa agtcgcacgc tgaaaggcca caaaatcggt
    16861 ctgacctcga aagcgatgca ggcaagctcg cagatcagcg aaccggatta cggtgcactg
    16921 atggacgaca tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg
    16981 cgcattgaag tggagctggc ttttgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg
    17041 ctgttcgacg tttacaacgc cacggactat gtgatcccgg cgctggagct gatcgacgct
    17101 cgctgccaca acatcgatcc ggaaacccag cgcccgcgca aagtattcga caccatttcc
    17161 gataacgccg ccaacgccgg agtgatcctc ggaggtcgtc cgatcaaacc agacgaactg
    17221 gatctgcgtt ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc
    17281 gccgctggtg tgctgaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca
    17341 ccgtatgacg tacaactgga agccgggcaa atcattctcg gtggttcgtt cacccgcccg
    17401 gttccggcgc gtaagggcga caccttccac gtcgattacg gcaacatggg ctccattagc
    17461 tgccgctttg tttaaggaga gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg
    17521 ccgtccgcag attggattat ggctggggct gagcagcagc tacagcgcgg agttactggc
    17581 cggaccagga ttcgactggt tattgatcga cggtgagcac gcgccgaata acgtgcaaac
    17641 cgtgctcacc caactacagg cgattgcgcc ctaccccagc cagccagtgg tacgcccatc
    17701 gtggaacgat ccggtgcaaa tcaaacaact gctggacgta ggcacacaaa ccttactggt
    17761 gccgatggta caaaacgccg acgaagcccg taaagcggta cgcgccaccc gttatccccc
    17821 cgccagtatt cgcggtgtgg gcagtgcgct ggctcgcgcc tcgcgctgga atcgcattcc
    17881 tgattacctg caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga
    17941 ggcaatgaag aacttaccgc agattctgga cgtggaaggg gtcgacggcg tgtttatcgg
    18001 cccggcggat ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca
    18061 ggccgccatt gagcaggcga tcgtgcagat ccgcgaagcg ggcaaagcgc cggggatcct
    18121 gatcgccaat gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt
    18181 cggcgttgac accaccctgc tcgcccgcgc cgccgaagcg ctggcagcac ggtttggtaa
    18241 tagttcttct atttcgatgg cgaagcaaaa caacaactct gtctactaaa cgtacgatgt
    18301 ggagcgcatc atgacgacct ctaccctgca atataaagat aataaaactg ttgaaattga
    18361 acatcgtgtc attaataaat tatttcgtcg cctaatcatt tttcttttta ttctgtttgt
    18421 tttttcgttt cttgatcgca tcaatatagg atttgccgga ctgacaatgg gcaaggatct
    18481 tggcctgacg tccacaatgt ttggcctcgc cgcaacactc ttttatgtca cgtacgtcat
    18541 gtgcgggatc cccagtaata tgatgttagc gaaaattggc gctcgtcgat ggatcgctgg
    18601 gatcatggtg gtctggggaa ttgcttccac ctgcacaatg tttgcaacaa gccccgaaac
    18661 actctatcta ctgcgtatgc tggttggtat tgctgaggcg ggttttttac ctggtatttt
    18721 ggtttatctg acctggtggt tcccggcata tcatcgggcc agagccaatg ccttatttat
    18781 gatagcgatg ccagtcacta tgatgctggg attgatcctt tcaggctaca ttctggcctt
    18841 ggatggactg tggaatctaa aaggctggca atggctcttt ttgctggaag gtctgccatc
    18901 ggtcattctt ggtgtcgtca cctggtttta tctcaacgac acaccagacc aggcgacctg
    18961 gcttgatgaa aacgaaaaac aggctttaaa aacgatgatt ctccgtgaac aagagagtgc
    19021 atttgcccat gcatctgttc cacgctcaac cttacgtgaa gtcctgaccc cagccatcat
    19081 gctatataca cttgcttatt tttgcctgac taatactctg agtgcgatta atatctggac
    19141 gccgcaaatc ctacagagtt tcaatactgg cagtagcaac atcatgattg gtttgctggc
    19201 ggccatcccc caattctgca caatttttgg catgatctgg tggagtcgtc gttccgaccg
    19261 tcttaaagaa cgcaaaaagc atacgattct tccctacctg tttgccgctg ccggatggtt
    19321 actcgcttct gcgacagatc acagcctcat tcaactgagc agcatcatta tggcctcaat
    19381 gggatcattt accgcgatga ctatcttttg gacaacaccg gatcaagtta taagcttgca
    19441 atcgcgtgcc gttgcgatgg ctgtcatcaa tgctgtcggc aatgtaggtt cagcagtcag
    19501 tccattactt atcggtattt tgaaagatgc aaccggcagt tttagctcag gtttgtggtt
    19561 tgtagcagga ttgctggtac tgggcgcgtt agtcatgacc cgaatcccta tggggagtcg
    19621 ggaacactct ctttcatctg aacttatcgc accaaaaccg caataaggag tcatgatgtc
    19681 cagtgaagct atctttgaac atggttatga aaatattgat ttggtccggg aatatgatgc
    19741 ccgctacgct ggtgaagatg tgcattatga aacatttgcc cggttagcga atttctttgg
    19801 acgcgatatg cgccctcact ggcacgatag atattttcaa ctccattatc tggtcactgg
    19861 acgcattact ttacaactgg atgagcactt ctattctgtg cgcgcaccgc tttttgtact
    19921 aacaccacca tcggtgccac atactttctt tactgatcct gatagcgatg ggcatgttct
    19981 gaccgtacgt caggagttaa tttggcccct ggtagaaaag ctctggcctg gtagtggaga
    20041 tgccgcaatg ctacaaggta tctgtctgtc actggaggga atggaacata cacaggcggc
    20101 attaaatcat tattggccga taatatccct ggagtttcaa caaaataagc aaggacggga
    20161 aattctattg caatcactgg cgcaaagtat ttttacaatc ctactgcgtg aagcaccacc
    20221 aaatgatatt tcaacatgca gcgtgcgcgg cgagatgtgt ttattccaga agtttaatcg
    20281 gctgattgac gaaaaatatc gccaacattt aatggtccct gaatatgcaa caatgctcgg
    20341 tatgagtgaa tcacggctta ccgagctgtg ccgacgtttt gccaatcaat cgccgaagag
    20401 attgattttc gaacgagtga gccgggaagc cagacggctt ctgctctata gcgagcaaag
    20461 catcaaccag atagcccttg atcttggcta caaagatccc gcctatttcg cccgtttttt
    20521 caaccgaatg atgggttgcc caccaacaca attccgtggt cgctgaccat ttatcaacct
    20581 gagagactcg acatgaaaat atctttacgc tcactatttg tggcgtgcct gattgcgcca
    20641 gtggcattca cctacgcctc cgaaccgcaa taaatgaaaa ttgtactact tggaacgaaa
    20701 ggtggccctt ctttattaaa agctgaccgt ttaccgatgg caacggcact tattattggc
    20761 aataaaacct agttaattga tgctggttac ggggccagct tacaactggt aaaaaataat
    20821 attccactgc gccagattga taatatttta ctcacccatt tacacagtga tcatattctg
    20881 gactatccat cagtcttaat gaatgcatgg gctagcggtc tgcaggacaa aactattcat
    20941 gtattcggtc cgccaggcac ccgagccatg accgctgcca gttggcaggt cttcgatcgg
    21001 gacatcacac tgcgtatgga agaagaagga aaacccgatc ctcgcaaact cgttacagct
    21061 caagatgttg gtgaaggcgt agtttataaa gatgataccg tgaccgtgac tgcattaaag
    21121 gtccctcatt ctcctttccc tgacggtgag gcttttgctt atcgcttcga cacacaaggc
    21181 aagcgaattg tcttctcagg agatacctcg tactttcccc cactggcaac tttctcagaa
    21241 ggcgctgata tacttgtgca tgaggccgta catgagccct ccgtcgctaa gctggcagac
    21301 agtattggca atggtaaaac actggcaaaa gccattgctt ctcaccatac gactattgag
    21361 gatgtaggca aaattgccaa tgaagcaaaa gtgaaaaaac tcatccttag ccacctggta
    21421 ccaatcaccg ttccggatga aacatggaag caagaagcac agaaaactat tcaggcccta
    21481 tcgtcgttgg tcatgaaaat atgacgatta ccattccatt cgccccataa aaaattccgt
    21541 ccccgttagc ccttgttagc ggggaatatt atgatttgcc ttactgtaag ttgatcacga
    21601 tcgcagttta ctatttaact ccaccttgct gtcgaaaagt accaacgttc gcctgaaacg
    21661 tctcttcccg acaaccgcgt tcaacgcttc aattaaataa caaaaacaac acacaaaatt
    21721 aacaatcgta ttccgattaa tactgtagag gtcgacatga aaccagaaga tttccgcgcc
    21781 agtacccaac gtcctttcac cggggaagag tatctgaaaa gcctgcagga tggtcgcgag
    21841 atctatatct atggcgagcg agtgaaagac gtcaccactc atccggcatt tcgtaatgcg
    21901 gcagcgtctg ttgcccaact gtacgacgcg ctgcacaaac cggagatgca ggactctctg
    21961 tgttggaaca ccgacaccgg cagcggcggc tatacccata aattcttccg cgtggcgaaa
    22021 agtgccgacg acctgcgcca gcaacgcgac gccatcgctg aatggtcacg cctgagctat
    22081 ggctggatgg gccgtacccc agactacaaa gccgctttcg gttgcgcact gggcgcgaat
    22141 ccgggctttt acggtcagtt cgagcagaac gcccgtaact ggtatacccg tattcaggaa
    22201 actggcctct actttaacca cgcgattgtt aacccaccga tcgtcatttg ccgaccgata
    22261 aagtgaaaga cgtttacatc aagctggaaa aagagactga cgccgggatt atcgtcagcg
    22321 gtgcgaaagt ggttgccacc aactcggcgc tgactcacta caacatgatt ggcttcggct
    22381 cggcacaagt gatgggcgaa aacccggact tcgcactgat gttcgttgcg ccaatggatg
    22441 ccgatggcgt gaaattaatc tcccgcgcct cttatgagat ggtcgcgggt gctaccggct
    22501 cgccatacga ctacccgctc tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg
    22561 ataacgtact gatcccatgg gaaaacgtgc tgatctaccg cgattttgat cgctgccgtc
    22621 gctggacgat ggaaggcggt ttcgcccgta tgtatccgct gcaagcctgt gtgcgcctgg
    22681 cagtgaaact cgacttcatt acggcactgc tgaaaaaatc actcgaatgt accggcaccc
    22741 tggagttccg tggtgtgcag gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct
    22801 gggcattgag tgactcgatg tgttcagaag cgacgccgtg ggtcaacggg gcttatttac
    22861 cggatcatgc cgcactgcaa acctatcgcg tactggcacc aatggcctac gcgaagatca
    22921 aaaacattat agaacgcaac gttaccagtg gcctgatcta cctcccttcc agtgcccgtg
    22981 acctgaacaa tccgcagatc gaccagtatc tggcgaagta tgtgcgcggt tcgaacggta
    23041 tggatcacgt ccagcgcatc aagatcctca aactgatgtg ggacgccatt ggcagcgaat
    23101 ttggtggtcg tcacgagctg tatgaaatca actactccgg tagccaggat gagattcgcc
    23161 tgcaatgtct gcgccaggca caaagctccg gcaatatgga caagatgatg gcgatggttg
    23221 atcgctgcct gtcggaatac gaccagaacg gctggactgt gccgcacctg cacaacaacg
    23281 acgatatcaa catgctggat aagctgctga aataacgcag caggaggtta agatgcaatt
    23341 agatgaacaa cgcctgcgct ttcatgacgc gatggccagc ctgtcggcag cggtaaatat
    23401 tatcaccacc gagggcgacg ccggacaatg cgggattacg gcaacggctg tctgctcggt
    23461 cacggataca ccaccgtcgc tgatggtgtg cattaacgcc aacagtgcga tgaacccggt
    23521 ttttcagggc aacggcaagt tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc
    23581 gcgccacttc gcgggcatga caggcatggc gatggaagag cgttttagcc tctcatgctg
    23641 gcaaaaaggt ccgctggcgc agccggtgct aaaaggttcg ctggccagtc ttgaaggaga
    23701 gatccgcgat gtgcaggcga ttggcacaca tctggtgtat ctggtggaga ttaaaaacat
    23761 catcctcagt gcagaaggtc acggacttat ttactttaaa cgccgtttcc atccggtgat
    23821 gctggaaatg gaagctgcga tttaagctcc agccccttca cgcattcgtc gaaggggcat
    23881 ttttattgtg atctcaatca catatctacc cgatgaataa ttcttgccca accgctcacc
    23941 gccattcctg accgcttaca tccctaaaat aaccactcag ttatttacct tactttacgc
    24001 gcacgtatct ctggcaacat cactgcagga tagcggtcaa tttacctcct caaacgcaac
    24061 gcaaacctag aacggcttcg gcctatgatt aacaaattac tctatggatt ttggtttgca
    24121 ggaaggcagc aagtgagtga atccccggga gcttacaaaa gtaagtgtca gaggtgaacg
    24181 aacgcagctg ccgcacctgt aagccaaaag acgacgagta aaatgccagg ttttactatg
    24241 gatacgaaaa aactattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc
    24301 tgcctcgcgg tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc
    24361 gcctctgtgt cggtgtatct ggtggcgtat cgctactaca gtctgtacat cgcccagaag
    24421 gtgatgaaac tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac
    24481 tacgttccga ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct
    24541 ggtccgctgg tgggaccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg
    24601 ctgctggcgg gggtagtgct ggccggtgcg gtacaggact ttatggtgct gtttatctcc
    24661 tctcgccgta acggcgcatc tctcggtgag atgatcaaag aagagatggg gccagtaccg
    24721 gggactatcg cgctgtttgg ctgtttctta atcatgatca tcatcctcgc cgttctggcg
    24781 ctgattgtgg ttaaagccct ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc
    24841 gtaccgattg cgctgtttat gggtatctac atgcgcttta tccgtccggg gcgtgtgggt
    24901 gaagtctctg ttattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt
    24961 gctcacgatc cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg
    25021 ctgattggct atgcgtttgt ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc
    25081 gactatctgg caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg
    25141 gtgctgaacc cggaactgaa aatgcctgcg atgacccagt acattgacgg tactggcccg
    25201 ctgtggaaag gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct
    25261 ggcttccacg cgctgatctc ttccggtacg acgccgaaac tgctggctaa cgaaaccgac
    25321 gcgcgtttca ttggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg
    25381 gttgcagcgt ccatcatcga accgggtctt tacttcgcga tgaacacccc gcctgctggc
    25441 cttggcatca ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg
    25501 gcgcagctga aagacgttac cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg
    25561 atttcgccag agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac
    25621 cgtgcaggtg gtgcgccgac gctggcggta ggtatcgctc acgtgttcca caaagtgctg
    25681 ccgatggctg acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc
    25741 ctgaccgcgc tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt
    25801 aacttcatcc cgttcctgaa aaaaaccgac tctctggttg ccggtatcat cggtactgcg
    25861 ggctgtgtgg gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc
    25921 gttaagagcc tgtggccgct gttcggtatc tccaaccaga tgctggcagc cgtagcgctg
    25981 gtactgggca ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt
    26041 gttccggctg tatggctgct tatctgcacc acctgggcgc tgggtctgaa actgttcagc
    26101 accaacccgc agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct
    26161 aacggtactg acctgacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac
    26221 tacaccaacg caggcctgag tattctgttc ctgattgtgg tgtacagcat catcttctac
    26281 ggtttcaaaa cctggcttgc ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg
    26341 tacgttccaa tcccggaagg cggcgtgaag atctcttcgc accactaacc gtgtttagcc
    26401 ccgcttcggc ggggctttgt tcttatcaga gtgaactatg tttggtaact taggacaggc
    26461 aaaaaaatat ctcggccagg cggcgaagat gttgattggt attccagact acgacaacta
    26521 cgtcgagcat atgaagacca accatcccga caagccgtac atgagctatg aagaattctt
    26581 ccgcgaacgc cagaacgcgc gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta
    26641 atggagagag catgaacccg attgcagtta ccctactcac cggtttttta ggcgcgggca
    26701 aaactaccct gctgcgccat attcttaacg aacaacacgg ctacaagatt gccgtgattg
    26761 aaaacgaatt cggcgaagtc tccgttgatg atcaactgat tggcgaccgc gccacgcaga
    26821 tcaaaacgct gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaggacgcac
    26881 tactcgacct gctggataat ctcgacaagg gcaatattca gttcgatcgt ctggtgattg
    26941 aatgcaccgg catggccgat cccggcccga ttattcagac ctttttctcc catgagattt
    27001 tatgccagct ctacctgctg gacggcgtga ttgcgctggt ggatgcggta catgccgatg
    27061 agcagatgaa ccagttcacc atcgcccagt cccaggtggg ctacgccgac cgtattctgc
    27121 tgactaaaac cgacgtcgca ggcgaagcag agaaactgcg tgaacgcctg gcgcgcatca
    27181 acgcccgcgc accggtctac accgtcaccc acggcgacat cgacctgggc ctgctgttca
    27241 acaccaacgg ttttatgctg gaagagaacg tcgtcagcac caaaccgcgt ttccacttta
    27301 tcgcggataa acaaaacgat atttcgtcaa ttgtggtgga actggattac ccggtagata
    27361 tcagcgaagt ttcccgcgtg atggaaaacc tgctgctgga atcggcggat aaactgctgc
    27421 gttacaaagg gatgctgtgg attgacggcg aacctaaccg cctgctgttc cagggcgtcc
    27481 agcgcctcta cagcgccgac tgggacaggc cgtggggcga tgaaaaaccg catagcacaa
    27541 tggtgtttat cgggattcag ttgccggagg aagagattcg ggcggcgttt gcgggattga
    27601 ggaagtaagt tcgggaaaga tcaacttttt gagagttcca ccgcaacatt attacgagtg
    27661 gaactcttta gatatgtcag accaaccaaa gcttcttcaa ttgcgttttc attttttcat
    27721 gtctcatttt cagttcagcc aatccccatt ctgaagaatt acaaacatct tttgttaaat
    27781 aaaaagagct tttactatta agtttctcat aaatagattt ctttttgata aaatcatagt
    27841 gttgtgtttc agaatttttg gaaccactta tgagtgttaa attacctatt ttatgcagcc
    27901 agtagacatg ctcttgaggt tggaatctat taatccagta ttcattaact aatgcttgag
    27961 gaagaatatg ctcaattgtg attctccccg tataggtttt aatcacgctc tcatcttgtt
    28021 gctccatatc taagcgaagc aaaattgctt ttattaagtt cacttgattc ggacgaggct
    28081 catataaatc ttcatccaga gcagctataa aacctgaatt atctgcatgt tggtttatct
    28141 gatttatcac agaatcaaaa ggcatatcat tatttattgc taccagagca gaataacaaa
    28201 ccatttctct ttggctttta atttgttttt ttaaccaccc atgcatatat accttctcaa
    28261 aggcggtaat aaattgagaa aaatcatcta aattaaaatc ttctgtacga gccattcgat
    28321 tcatgaatgc cataaccgga ggaatccact catctacccc taaattactt agcgatgcta
    28381 tttttcgtcg tattgaaggg tgttcaaaat cattctctaa aatcttagt
//
LOCUS       DAJVRL010000051        28338 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVRL010000051 DAJVRL010000000
VERSION     DAJVRL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.051"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="OQN02_002165"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="OQN02_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="OQN02_002166"
     CDS             complement(998..2020)
                     /locus_tag="OQN02_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1642947.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="OQN02_002167"
     CDS             complement(2034..3536)
                     /locus_tag="OQN02_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1642948.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="OQN02_002168"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="OQN02_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="OQN02_002169"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="OQN02_002169"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1642949.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="OQN02_002170"
     CDS             complement(5851..6192)
                     /locus_tag="OQN02_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1642950.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="OQN02_002171"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="OQN02_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1642951.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="OQN02_002172"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="OQN02_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1642952.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="OQN02_002173"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="OQN02_002173"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1642953.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="OQN02_002174"
     CDS             12871..14214
                     /locus_tag="OQN02_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1642954.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="OQN02_002175"
     CDS             complement(14276..14482)
                     /locus_tag="OQN02_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1642955.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="OQN02_002176"
     CDS             14807..15361
                     /locus_tag="OQN02_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1642956.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="OQN02_002177"
     CDS             complement(15424..16362)
                     /locus_tag="OQN02_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1642957.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="OQN02_002178"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="OQN02_002178"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1642958.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="OQN02_002179"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="OQN02_002179"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1642959.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="OQN02_002180"
     CDS             complement(19573..19953)
                     /locus_tag="OQN02_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1642960.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="OQN02_002181"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="OQN02_002181"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1642961.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="OQN02_002182"
     CDS             21011..21976
                     /locus_tag="OQN02_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1642962.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="OQN02_002183"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="OQN02_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1642963.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="OQN02_002184"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="OQN02_002184"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1642964.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="OQN02_002185"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="OQN02_002185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1642965.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="OQN02_002186"
     CDS             25350..25895
                     /locus_tag="OQN02_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCS1642966.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="OQN02_002187"
     CDS             complement(25879..26526)
                     /locus_tag="OQN02_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1642967.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="OQN02_002188"
     CDS             26738..27529
                     /locus_tag="OQN02_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCS1642968.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28338
                     /locus_tag="OQN02_002189"
     CDS             27539..>28338
                     /locus_tag="OQN02_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCS1642969.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6953 a         7541 c         6989 g         6855 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgctgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaagat attaacag
//
LOCUS       DAJVRL010000052        27556 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVRL010000052 DAJVRL010000000
VERSION     DAJVRL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.052"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQN02_002190"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQN02_002190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1642970.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQN02_002191"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQN02_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1642971.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQN02_002192"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQN02_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1642972.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OQN02_002193"
     CDS             3807..3995
                     /locus_tag="OQN02_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1642973.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OQN02_002194"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OQN02_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1642974.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OQN02_002195"
     CDS             4756..5115
                     /locus_tag="OQN02_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS1642975.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OQN02_002196"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OQN02_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OQN02_002197"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OQN02_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1642976.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OQN02_002198"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OQN02_002198"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1642977.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10895..11071
                     /locus_tag="OQN02_002199"
                     /pseudo
     CDS             10895..11071
                     /locus_tag="OQN02_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            11399..13147
                     /gene="bcsC"
                     /locus_tag="OQN02_002200"
                     /pseudo
     CDS             11399..13147
                     /gene="bcsC"
                     /locus_tag="OQN02_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            13229..15217
                     /gene="hmsP"
                     /locus_tag="OQN02_002201"
     CDS             13229..15217
                     /gene="hmsP"
                     /locus_tag="OQN02_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1642978.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            15402..16688
                     /gene="dctA"
                     /locus_tag="OQN02_002202"
     CDS             15402..16688
                     /gene="dctA"
                     /locus_tag="OQN02_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1642979.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            16909..18405
                     /locus_tag="OQN02_002203"
     CDS             16909..18405
                     /locus_tag="OQN02_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1642980.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="OQN02_002204"
     CDS             complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="OQN02_002204"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1642981.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            19662..20429
                     /gene="pdeH"
                     /locus_tag="OQN02_002205"
     CDS             19662..20429
                     /gene="pdeH"
                     /locus_tag="OQN02_002205"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1642982.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            20499..22559
                     /locus_tag="OQN02_002206"
     CDS             20499..22559
                     /locus_tag="OQN02_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1642983.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(22741..24063)
                     /locus_tag="OQN02_002207"
     CDS             complement(22741..24063)
                     /locus_tag="OQN02_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1642984.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="OQN02_002208"
     CDS             complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="OQN02_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1642985.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(25536..26435)
                     /locus_tag="OQN02_002209"
     CDS             complement(25536..26435)
                     /locus_tag="OQN02_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1642986.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26955..>27556
                     /locus_tag="OQN02_002210"
     CDS             26955..>27556
                     /locus_tag="OQN02_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1642987.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6565 a         6951 c         7551 g         6489 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccaag
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaataatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgtgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caactctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtagtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactttcgc aggatctctg gcaggccgga caacgcagcc aggccgatac
    11221 gttaatgcgc aatctggcgc agcagaagcc gaacaacccg gagcaggttt acgcttacgg
    11281 gctgtacctc tcaggtcaca accaggacag agcggcgctg gcgcatatca acagcctgcc
    11341 gcgcgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa gcgatcaggt
    11401 gctggaaacc gctaatcgcc tgcgagaaag cggcaaagag gctgaagcgg aagcgatgct
    11461 gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg cgtaacaacg
    11521 acgtgattac accgccgccc gcactgcata tcagaatgtc ctgacgcggg agccaactaa
    11581 cgccgatgcc attcttggtc tgacggaagt ggatattgct gccggtgaca aagcggcggc
    11641 acgtagccag ctggcgaaac tgcctgccac cgataacacc tcgctgaaca ctcagcggcg
    11701 cgtggcgctg gcacaggcgc agcttggaga taccgcagcg gcgcagcaga cgtttaataa
    11761 gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg tactgcgcga
    11821 cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag gcgctggaaa cctacaaaga
    11881 cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca cctttacccg
    11941 actgacccgt aacgacgaga aagatgactg gctgaaacgc ggcgtgcgca gcgatgcggc
    12001 ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact ggggttcgag
    12061 cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg tggatgcgcc
    12121 gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg tcggcagttt
    12181 ctccactaat gccgatggca aatgggatga caactggggc acctgtacat tacaggactg
    12241 tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct ggcgaaatga
    12301 cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg atgtggtcgg
    12361 cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg cccaccgtcg
    12421 gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga gcaataccgg
    12481 gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct acgataaagg
    12541 tgaagtaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta aaaatgtcga
    12601 agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta accagaacaa
    12661 tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag atctgagtgg
    12721 ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt ttgccatacc
    12781 ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt ctggctcgtg
    12841 gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga tcccgaccga
    12901 ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg gcttcggcta
    12961 cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg gtacggcaat
    13021 tgatatccag caggcgaaag attacgcacc cagccatttc ctgctctacg tacgttattc
    13081 cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac cttacgccga
    13141 ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat actcgggcgg
    13201 caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa caatcaagca
    13261 gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca ccgttttgct
    13321 gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa gcattgctcg
    13381 ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg aagcggaagc
    13441 tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa
    13501 ccagttccag gcgctgcgta aaagttttat tccagagcgt ccggtgccgg taatggttac
    13561 tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtc tactcgctcg aacgtccggc
    13621 aaatccgcag ccaatagcct atctggtgct acaggcggat tccttccgta tgtataagtt
    13681 cgtgatgagc actctctcaa cgttagtgac catttactta cttttgtcgc ttatcctgac
    13741 cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca atattgctcg
    13801 cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat taccgcgtct
    13861 gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc agcaattgct
    13921 gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc
    13981 gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac aaaccaccgc
    14041 gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca
    14101 acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt cgccacgtat
    14161 gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg tacaggaacc
    14221 gtggcacgca atcactttgg gtcagcaagt actcactatc atgagcgagc gcctgccgat
    14281 tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct acggcgatct
    14341 caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc gccataaagg
    14401 caagaatcag attcagttct ttgatccgca gcagatggaa gcagcccaga agcggttgac
    14461 ggaagagagc gatatcctta atgcactgga aaatcatcag tttgcaattt ggttacagcc
    14521 acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc gtatccagca
    14581 accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt gctgtgggct
    14641 gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg cagcctggca
    14701 agagcgcggc attatgctgc ccttgtcggt aaacttctct gcgctgcaac tgatgcaccc
    14761 gaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc cgggaacact
    14821 gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg tggcaatcct
    14881 ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca tgggctacgc
    14941 agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga aaatcgacaa
    15001 aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta tcatgctggc
    15061 gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac aacgagactg
    15121 gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcgcc cactccctat
    15181 tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg attacaaaac
    15241 tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt gtttcaatta
    15301 tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc aggtaccccc
    15361 cataacctta caagacctgt ggttttacta aaggacaccc tatgaaaacc tctctgttta
    15421 aaagccttta ctttcaggtc ctgacagcga tagccattgg tattctcctt ggccatttct
    15481 atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag ctcattaaga
    15541 tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc atggaaagca
    15601 tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt gtcagtacca
    15661 tcgcgctgat tattggtctt atcatcgtta acgtcgtgca gcctggtgcc ggaatgaacg
    15721 tcgatccggc aacgcttgat gcgaaagcgg tagcggttta cgccgatcag gcgaaagacc
    15781 agggcattgt cgccttcatt atggatgtca tcccggcgag cgtcattggc gcatttgcca
    15841 gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg ctccaccgtc
    15901 tgggcagcaa aggccaactg atttttaacg tgatcgaaag tttctcgcag gtcatcttcg
    15961 gcatcatcaa tatgatcatg cgtctggcac ctattggtgc gttcggggca atggcgttta
    16021 ccatcggtaa atacggcgtc ggcacactgg tgcaactggg gcagctgatt atctgtttct
    16081 acattacctg tatcctgttt gtggtgctgg tattgggttc aatcgctaaa gcgactggtt
    16141 tcagtatctt caaatttatc cgctacatcc gtgaagaact gctgattgta ctggggactt
    16201 catcttccga gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc ggctgccgta
    16261 aatcggtggt ggggctggtt atcccgacag gctactcgtt taaccttgat ggcacatcga
    16321 tatacctgac aatggcggcg gtgtttatcg cccaggccac taacagtcag atggatatcg
    16381 tccaccaaat cacgctgtta atcgtgttgc tgctttcttc taaaggggcg gcaggggtaa
    16441 cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc ggtgggccat ttgccggtag
    16501 cgggtctggc gctgatcctc ggtatcgacc gctttatgtc agaagctcgt gcgctaacta
    16561 acctggtcgg taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa gaactggacc
    16621 acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg cacgaattat
    16681 cctcttaatc tcaccactta tgcccgcact cccttccccg agtgcgggtg tttgcgcata
    16741 attgacccct gtgcctgaac ttaccacctg ggtgagttca ggtttatttc acttcttccg
    16801 tgacattttc atgttcttgc ggtctaacac gaagtgtttt taacgtcatt tagactgacc
    16861 gacaacgggc agttttttta ttaccaggat agttgatcag gggttcacat gcagggcata
    16921 aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta tgtgcaggca
    16981 gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg tttacagtgg
    17041 caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct gctggttaat
    17101 accggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat ccctcgtatt
    17161 gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg gcagcagggg
    17221 atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac gctgtttaat
    17281 ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta tctggcaaat
    17341 gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca aagtcaggac
    17401 atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg tctgaaaggg
    17461 tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga agcggaaaaa
    17521 attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact ggtggtggga
    17581 aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga actgaaaggc
    17641 aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt
    17701 attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac gccgtggcag
    17761 ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc ccgcgaggcg
    17821 ctgttctggc atgttcagca agcgttaagc gccagtgaca gcaaagacat cggtcttgga
    17881 tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac
    17941 gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa
    18001 ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga gctgcagaaa
    18061 ctgtttgccg cctatgcacg ggctgatacc gatattctga tgggccagcg gatgcgttcg
    18121 ttgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg gcaggatttc
    18181 cttaacagcc tgactgtgga gatgttaaat caggatctgc gtcagcagtt gtcgaatgat
    18241 atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa agcgttgcag
    18301 gcggcctggg atcaaatcat ggccccatct accgccgctg cgaccacctc tgtcgccacg
    18361 gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct gatggtgatg
    18421 agttagcaca accatcaggt atttatggat gagctggata gaggggtaac actttatcca
    18481 gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat cgcgccgcga
    18541 tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc cgcactgccg
    18601 cccgtcaaac gtaccgccag ataaccggcg ctgaaagagt cgccagctgc ggtggtatcg
    18661 atcacttttt ctttcggcag tttcaccgcc ggaacatcaa ctaacgcttc accagcaatg
    18721 gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc cgcgttatgg
    18781 gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc gtcgtccagc
    18841 gtcaggaagg cgatatccgt gcattccagc atttgctggt acacctgctg tgtctcttct
    18901 ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc gcctttggcg
    18961 cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg
    19021 ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat cgccgcagac
    19081 tgctcgctcg ccagccagaa tttggcggcg gcttcgttac gccagtagta gaacgtacgt
    19141 tcgccggtgc tgtcggtttc aatgtagtaa aggcccggaa gacggttttc catccgttgg
    19201 gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatctgctg gctaaaactg
    19261 tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac ctgacgggcg
    19321 atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc cgcgcctttc
    19381 tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat agtcgtgaac
    19441 ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc aaccatatta
    19501 aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc cgttgtggag
    19561 tgaggaaatt taccccggcc aggcggtaaa gttctgccct tacgcgccga taatctttgt
    19621 cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg caggttatcc
    19681 agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt ttttggttgc
    19741 agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcgggcgg ttaatggccg
    19801 tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg ccgccggatc
    19861 gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag cagattgatt
    19921 tgctggcgca aaaagccgac ttctttgtag agcacggcct gctggcatcg gtcaatattg
    19981 atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccag attgagcgtc
    20041 ttccctggct gcgtttcgaa ctggtggagc atatccgtct gccgaaagat tcaacctttg
    20101 cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggtaccggg atggcaaatt
    20161 tctctgcact aagtgaagtg cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga
    20221 tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat ctaatgaatc
    20281 gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg cgtgatgttc
    20341 agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca ccgatggaaa
    20401 cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgccggg ctggactatc
    20461 tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca ggcaaaataa
    20521 ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa
    20581 cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg gagttgaatc
    20641 gtccgttcgc tatccgtggc aatctgggcg tggtgtggga gcggcaaaaa caagaaactg
    20701 gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt cttggtaatc
    20761 caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca acgctggcac
    20821 cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa aagcccgacg
    20881 cgcgcctgat tcgcctctct gaaaagaaca ataactggac gtttaatctt gccaacgatg
    20941 acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat aatattcttt
    21001 tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg gaaatttttg
    21061 ttgatccgtt aggcaagccg ctgccattca gcgaagttac tggatcgaaa ggtaaagcgg
    21121 ataaagaaaa ggtgggcgat tacatttttg gcctgaaggc gcagggacgt tataacggtg
    21181 aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc gaagggacgc
    21241 cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt gatggcgtcg
    21301 tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct ggcgattcac
    21361 tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg tttgaaacgg
    21421 atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat tatcgcggtt
    21481 ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc accggaaagc
    21541 cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg gacctgggac
    21601 cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct gaacagaaga
    21661 agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg
    21721 ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt gagcatggca
    21781 gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct gacctgcgcc
    21841 tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt catctggaag
    21901 gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg aaactgaaag
    21961 aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg
    22021 aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac ggcaacctga
    22081 aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt gggctgaatg
    22141 tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg aactgcgcgg
    22201 cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct ttcgatactg
    22261 agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcggaacag ctggatttga
    22321 ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg ctgtatgtgc
    22381 gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt gcccgtggtg
    22441 ctgttgctgc ggcactggca acgctggtaa caccggcggc ggcgttactg gcactgatct
    22501 caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg aagaagtgat
    22561 gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt ggcaaagtgt
    22621 gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc cttgtgaatt
    22681 caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt ttcacactta
    22741 ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg tcaagccagc
    22801 catcgccgcc agatataacc ccaccgcacc taacccgtag ttagcctgca accaggctgc
    22861 gatatacggc gcaacggaag ccccgagaat cgacgatacg ttgtaagaga acgatgctcc
    22921 ggtgtaacgc acttctgtcg gaaacagctc tggtaacagc gcacccatcg gcccgaaggt
    22981 cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg ggttgccaga
    23041 accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg tgatgatcac
    23101 catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca ccatcacgcc
    23161 aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca ggccaagccc
    23221 aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt aaaacagcgt
    23281 ataggttgcc agcataatga acgtacccag tacggttacg cgaacatgtt tggtcagcag
    23341 cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa acaccggcga
    23401 ctcatgcagc gacacgcgaa catacaggcc gataatgacc agcaccgccg agaagataaa
    23461 tggcacacgc cagccccagc tcataaactg ctcatcggtc agtagccagg aaagcagcaa
    23521 gaaagtgccg ttggcaaaga agaagccaat cggtgcgcct agctgtggga aggagccata
    23581 cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcac cgccccattc
    23641 accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagtg gggcgaaaat
    23701 accaatcgtg gcatagcccg gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag
    23761 caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc
    23821 agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc
    23881 tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg ccgcagtggc
    23941 gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga caaggacttt
    24001 attacgcgag ttgatcggcg tgtattcttg ttcgtggtcg agtgttgtgg ctgttgcttg
    24061 cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc aaaagtgata
    24121 tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg gataatgttt
    24181 aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag tggataaaac
    24241 tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta tgagttggag
    24301 tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt ttcaagaggt
    24361 tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta cggggagcca
    24421 ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct attttaaggc
    24481 tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg
    24541 caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag cacggaacca
    24601 aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat cacgatttta
    24661 atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt tttgcgcgga
    24721 cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc gaagatggaa
    24781 atcgccaggc caatcaatcg ccacgtcggt ttcagccact caatgctgtt caggtgcagg
    24841 gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag cgtcacaatc
    24901 agcgcaatca acaaaccaat cagtgaaata aaatcacgca gatattttac ccagaacttc
    24961 tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc
    25021 aggttaccca tccagttgat gccggaataa agtgccaccg ccaggccgac aagccctaca
    25081 gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc tagtgtcgga
    25141 tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg atgggaggcc
    25201 agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga caaaaatgag
    25261 aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg ttctgtcgcg
    25321 cgaataaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt ggtgacggtt
    25381 tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt aatcggctcg
    25441 tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc
    25501 cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt ccaggtgctc tttcattacg
    25561 ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa
    25621 accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat aagccgcccg
    25681 gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag accggcaaga
    25741 ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga cttaaaccac
    25801 tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag gtgcggtgta
    25861 taacgcacta ttgcgtgtga agtcagatca tcggggcttt gcggataacc aaagcgcgtc
    25921 agatagtgcg gactggcaca gttgaccatg gttagtttgc cgaggggacg ggcgataacg
    25981 ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg aagaatatct
    26041 accggacggt tatggctact cagttccagc tcaattcccg gatgctgata gagaaattcc
    26101 gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat gcgtaattta
    26161 ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag attactcaac
    26221 acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac ccgtcgcgta
    26281 gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg gcgcgaaaca
    26341 cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag ctccgcgact
    26401 ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg ttgttcacag
    26461 cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta atgccgggtt
    26521 tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg gaatagcttt
    26581 atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct ttaaagtgaa
    26641 tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt tattttggct
    26701 tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat aaggaaatcc
    26761 tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc tgtaactatt
    26821 tatatcggta actattttct actcgtcatc tctgtagaat ggccaccgcc aaatggcgaa
    26881 ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta acgtattcag
    26941 gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt attcatggaa
    27001 taaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggtc agtcaggcag
    27061 acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc gatggcgatc
    27121 aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt cctgaggtta
    27181 aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt atccatttta
    27241 atgtgctggc cattgtgcct cgcgattcaa ccgtcgagac atttgcgttg gcggtaaata
    27301 gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg gaaaaggaca
    27361 ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg ttagcggcgg
    27421 gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg gtaaaagcgc
    27481 atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg gcaatgatgt
    27541 taaatatctc ctcctg
//
LOCUS       DAJVRL010000053        27537 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVRL010000053 DAJVRL010000000
VERSION     DAJVRL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.053"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OQN02_002211"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OQN02_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1642988.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OQN02_002212"
     CDS             complement(1614..1937)
                     /locus_tag="OQN02_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1642989.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQN02_002213"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQN02_002213"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1642990.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQN02_002214"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQN02_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1642991.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQN02_002215"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQN02_002215"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1642992.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQN02_002216"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQN02_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1642993.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OQN02_002217"
     CDS             7537..8574
                     /locus_tag="OQN02_002217"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1642994.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQN02_002218"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQN02_002218"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1642995.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OQN02_002219"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OQN02_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1642996.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQN02_002220"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQN02_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1642997.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OQN02_002221"
     CDS             10745..11626
                     /locus_tag="OQN02_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1642998.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQN02_002222"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQN02_002222"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1642999.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OQN02_002223"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OQN02_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1643000.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDVVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQN02_002224"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQN02_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OQN02_002225"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OQN02_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1643001.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OQN02_002226"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OQN02_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1643002.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OQN02_002227"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OQN02_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1643003.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OQN02_002228"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OQN02_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1643004.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OQN02_002229"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OQN02_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1643005.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OQN02_002230"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OQN02_002230"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1643006.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OQN02_002231"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OQN02_002231"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1643007.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OQN02_002232"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OQN02_002232"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1643008.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OQN02_002233"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OQN02_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1643009.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OQN02_002234"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OQN02_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1643010.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQN02_002235"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQN02_002235"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1643011.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OQN02_002236"
     CDS             24893..25273
                     /locus_tag="OQN02_002236"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1643012.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OQN02_002237"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OQN02_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1643013.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQN02_002238"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQN02_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1643014.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27537
                     /locus_tag="OQN02_002239"
                     /pseudo
     CDS             26442..27537
                     /locus_tag="OQN02_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6797 a         6854 c         7219 g         6667 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgtcgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgataaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaa
//
LOCUS       DAJVRL010000054        27519 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVRL010000054 DAJVRL010000000
VERSION     DAJVRL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.054"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OQN02_002240"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OQN02_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCS1643015.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OQN02_002241"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OQN02_002241"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1643016.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OQN02_002242"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OQN02_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1643017.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OQN02_002243"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OQN02_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1643018.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OQN02_002244"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OQN02_002244"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1643019.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OQN02_002245"
     CDS             6426..7055
                     /locus_tag="OQN02_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1643020.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OQN02_002246"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OQN02_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1643021.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8730..10073)
                     /gene="melA"
                     /locus_tag="OQN02_002247"
     CDS             complement(8730..10073)
                     /gene="melA"
                     /locus_tag="OQN02_002247"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1643022.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OQN02_002248"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OQN02_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1643023.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OQN02_002249"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OQN02_002249"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1643024.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="OQN02_002250"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="OQN02_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1643025.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="OQN02_002251"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="OQN02_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1643026.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="OQN02_002252"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="OQN02_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1643027.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="OQN02_002253"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="OQN02_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1643028.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="OQN02_002254"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="OQN02_002254"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1643029.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OQN02_002255"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OQN02_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1643030.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OQN02_002256"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OQN02_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1643031.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="OQN02_002257"
     CDS             complement(21746..22624)
                     /locus_tag="OQN02_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS1643032.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OQN02_002258"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OQN02_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1643033.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25251..25586
                     /locus_tag="OQN02_002259"
     CDS             25251..25586
                     /locus_tag="OQN02_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1643034.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25744..26187
                     /gene="yjdN"
                     /locus_tag="OQN02_002260"
     CDS             25744..26187
                     /gene="yjdN"
                     /locus_tag="OQN02_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCS1643035.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26320..27108
                     /gene="phnC"
                     /locus_tag="OQN02_002261"
     CDS             26320..27108
                     /gene="phnC"
                     /locus_tag="OQN02_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1643036.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27133..>27519
                     /gene="phnD"
                     /locus_tag="OQN02_002262"
     CDS             27133..>27519
                     /gene="phnD"
                     /locus_tag="OQN02_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1643037.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDS"
BASE COUNT         7066 a         7163 c         6696 g         6594 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agcactgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctgacagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac tagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgcaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctgg atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccgcgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgt atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 cgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctgc
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgca actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac caatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc attagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgtttttta tcagaataaa taacgtcttc tgatgcgttt aaaacgtcag aaagataaaa
    25021 atatcatgtg aataaaaaaa agaacaagta gagcattaac attatcttaa ataataaata
    25081 gaggcaaaaa gattattttc tttttacgtt tcctttcaaa tgaaaacgat cgtcgtctaa
    25141 aatcagcagt acccccgaca aactcaggga ttttgtgtat aattgcggcc tttttcggca
    25201 atctgccgtt ttttggcgct tttgccctgc tgacttttga ggaaatccac atgtcattac
    25261 cacactgccc aaaatgcaac tccgaataca cttacgaaga taacggcatg tacatctgcc
    25321 cggaatgtgc ctacgaatgg aacgacgcag aacctgcaca ggaaagcgac gagctgatcg
    25381 ttaaagatgc taacggcaat ctgctggctg acggcgacag cgttaccatc attaaagatc
    25441 tgaaggtgaa aggtagctct tcgatgctga aaattggcac caaagtgaaa aacatccgcc
    25501 tggttgaagg cgaccataac atcgattgca aaatcgacgg ttttggtccg atgaaactga
    25561 aatctgagtt tgtgaaaaag aactgattgt attgtgatcg gtaagccgga taaggcgctc
    25621 gcgccgcatc cggcaacgat gccgactgcc tgatgcgacg cttgcgcgtc ttatcaggca
    25681 gcctctcatc tgtataaatt tcgaactaca cttaactggc ttctcttaac tgaggtcacc
    25741 atcatgccgt taagtcccta cctctctttt gccggtaact gttccgacgc gattgcctat
    25801 tatcaacgta cgttgggcgc ggaactgctc tataaaatca gcttcggcga aatgccgaaa
    25861 tcagcgcagg acagcgccga gaactgccct tccggaatgc aatttcccga taccgccatc
    25921 gctcatgcca acgtgcgcat tgccggaagc gacatcatga tgagcgatgc tataccgtca
    25981 ggaaaagcca gctactccgg ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga
    26041 aaacgctggt ttgataacct tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact
    26101 ttctgggcgc atggctttgg caaagtcacc gataaatttg gcgtaccgtg gatgattaat
    26161 gtcgtcaaac aacaaccaac gcaataaccc gccgggaggc ccgccctccc gcactgtcat
    26221 cgaattcccg ttaactcttc atctgttagt cacttttaat taaccaaatc gtcacaataa
    26281 tccgccacga tggagccact tttttaggga ggctgcatca tgcaaacgat tatccgtgtc
    26341 gagaagctcg ccaaaacctt caatcagcat caagcgctgc atgcggttga tctgaacatt
    26401 catcacagtg aaatggtggc tctgcttggg ccgtcgggtt ccggcaaatc caccctttta
    26461 cgtcatttaa gcggtttgat taccggcgat aaatccgccg gtagccatat cgagctactg
    26521 ggccgcactg tccagcgcga aggccgcctg gcccgcgata tccgcaaaag ccgcgccaac
    26581 accggctaca tctttcaaca attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg
    26641 ctgattggcg cgctcggcag cacgccgttc tggcgcacct gttttagctg gttcaccggc
    26701 gagcagaaac agcgcgcgtt acaggcgctg acccgcgttg gcatggtgca ttttgcccat
    26761 cagcgcgttt ccaccctctc cggcggccag cagcaacgtg tggcgattgc ccgtgcgctg
    26821 atgcagcagg cgaaagtgat tctggccgat gaacccatcg cctcgctgga cccggaatca
    26881 gcgcgcatcg tgatggacac cctgcgcgac atcaaccaga acgacggcat caccgtggtc
    26941 gtcacgctgc atcaggtgga ttacgccctg cgctactgcg aacgcatcgt cgccctgcgt
    27001 caggggcacg tcttctacga cggcagcagc caactgtttg ataacgaacg ttttgaccat
    27061 ctctaccgca gcattaaccg catcgaagag aacgcgaaag ctgcctgata tccccatcat
    27121 tgaggaaaac gaatgaacgc taagataatt gcctcgctgg ccttcaccag catgttcagc
    27181 ctcagcaccc tgttaacccc ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc
    27241 attatttcaa cggaatcaca gcaaaacctg aaaccgcaat ggacgccgtt tttacaggat
    27301 atggagaaga aactgggcgt gaaggtcaac gccttctttg ccccggacta cgcgggcatt
    27361 atccagggga tgcgcttcaa taaagtggat attgcctggt acggcaacct gtcggcgatg
    27421 gaagcggtgg atcgcgccaa tggccaggtc ttcgcccaga cggtcgcggc ggatggatcg
    27481 ccgggttact ggagcgtgtt gatcgtcaac aaagacagc
//
LOCUS       DAJVRL010000055        26562 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVRL010000055 DAJVRL010000000
VERSION     DAJVRL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.055"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            348..641
                     /locus_tag="OQN02_002263"
     CDS             348..641
                     /locus_tag="OQN02_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1643038.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="OQN02_002264"
     CDS             713..1372
                     /locus_tag="OQN02_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1643039.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="OQN02_002265"
     CDS             1464..1910
                     /locus_tag="OQN02_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1643040.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OQN02_002266"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OQN02_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="OQN02_002267"
     CDS             3390..3809
                     /locus_tag="OQN02_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1643041.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="OQN02_002268"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="OQN02_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS1643042.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OQN02_002269"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OQN02_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1643043.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OQN02_002270"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OQN02_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1643044.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="OQN02_002271"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="OQN02_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1643045.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="OQN02_002272"
     CDS             complement(8332..9864)
                     /locus_tag="OQN02_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1643046.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="OQN02_002273"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="OQN02_002273"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1643047.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="OQN02_002274"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="OQN02_002274"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS1643048.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="OQN02_002275"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="OQN02_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="OQN02_002276"
     CDS             complement(14765..16501)
                     /locus_tag="OQN02_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1643049.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OQN02_002277"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OQN02_002277"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1643050.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="OQN02_002278"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="OQN02_002278"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1643051.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="OQN02_002279"
     CDS             complement(18223..18957)
                     /locus_tag="OQN02_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1643052.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="OQN02_002280"
     CDS             19158..19412
                     /locus_tag="OQN02_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1643053.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..20922)
                     /locus_tag="OQN02_002281"
     CDS             complement(19462..20922)
                     /locus_tag="OQN02_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1643054.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20859..21416)
                     /locus_tag="OQN02_002282"
     CDS             complement(20859..21416)
                     /locus_tag="OQN02_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCS1643055.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21442..22296)
                     /locus_tag="OQN02_002283"
     CDS             complement(21442..22296)
                     /locus_tag="OQN02_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCS1643056.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="OQN02_002284"
     CDS             complement(22296..23105)
                     /locus_tag="OQN02_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCS1643057.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="OQN02_002285"
     CDS             complement(23115..23873)
                     /locus_tag="OQN02_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1643058.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="OQN02_002286"
     CDS             complement(23870..24850)
                     /locus_tag="OQN02_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCS1643059.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="OQN02_002287"
     CDS             complement(24850..25872)
                     /locus_tag="OQN02_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCS1643060.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26221..>26562
                     /locus_tag="OQN02_002288"
     CDS             26221..>26562
                     /locus_tag="OQN02_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643061.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6683 a         6472 c         6850 g         6557 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa taggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagaatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacacgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcact cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttaggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt actcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgctggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt tttccctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gagcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattaccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgga
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 caccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggagaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat catttaaata tgaatgacat tattgttttt tatcgtccac
    26101 ggttacgcga cgttgaagtt acttgtttta taatgagcga tttttgatat ctcgccgaga
    26161 aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga
    26221 atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct tgctgatagt
    26281 aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa cgacgttatt
    26341 aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt actgaatgcg
    26401 tttattcgga cgtgcaaaaa aagagatgat aacagttacg gaaatatgat atttacgctt
    26461 tattttgata acgatatcta tgggaacaga attatcaaag catcgctgaa cggcggagag
    26521 ttgcagttat taagcacagg gtgtagcaat gataacattc tc
//
LOCUS       DAJVRL010000056        26076 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVRL010000056 DAJVRL010000000
VERSION     DAJVRL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.056"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OQN02_002289"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OQN02_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1643062.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OQN02_002290"
     CDS             complement(2138..2584)
                     /locus_tag="OQN02_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1643063.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OQN02_002291"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OQN02_002291"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1643064.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OQN02_002292"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OQN02_002292"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1643065.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OQN02_002293"
     CDS             complement(5347..6009)
                     /locus_tag="OQN02_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1643066.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OQN02_002294"
     CDS             6182..6595
                     /locus_tag="OQN02_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1643067.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OQN02_002295"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OQN02_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1643068.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OQN02_002296"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OQN02_002296"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCS1643069.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OQN02_002297"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OQN02_002297"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1643070.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OQN02_002298"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OQN02_002298"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1643071.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OQN02_002299"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OQN02_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1643072.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OQN02_002300"
     tRNA            complement(9861..9948)
                     /locus_tag="OQN02_002300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OQN02_002301"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OQN02_002301"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1643073.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OQN02_002302"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OQN02_002302"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS1643074.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OQN02_002303"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OQN02_002303"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCS1643075.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OQN02_002304"
     CDS             14005..14592
                     /locus_tag="OQN02_002304"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1643076.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OQN02_002305"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OQN02_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1643077.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OQN02_002306"
     CDS             14984..15841
                     /locus_tag="OQN02_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1643078.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OQN02_002307"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OQN02_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1643079.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OQN02_002308"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OQN02_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1643080.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OQN02_002309"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OQN02_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1643081.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OQN02_002310"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OQN02_002310"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1643082.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OQN02_002311"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OQN02_002311"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS1643083.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OQN02_002312"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OQN02_002312"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1643084.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="OQN02_002313"
     CDS             complement(22898..24037)
                     /locus_tag="OQN02_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS1643085.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="OQN02_002314"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="OQN02_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..26017)
                     /locus_tag="OQN02_002315"
     CDS             complement(25322..26017)
                     /locus_tag="OQN02_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1643086.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6217 a         6648 c         6906 g         6305 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcgga
//
LOCUS       DAJVRL010000057        25973 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVRL010000057 DAJVRL010000000
VERSION     DAJVRL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.057"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..346)
                     /locus_tag="OQN02_002316"
     CDS             complement(<1..346)
                     /locus_tag="OQN02_002316"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1643087.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHKVTTTVSKNH"
     gene            complement(479..784)
                     /locus_tag="OQN02_002317"
                     /pseudo
     CDS             complement(479..784)
                     /locus_tag="OQN02_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            852..1467
                     /gene="tnpA"
                     /locus_tag="OQN02_002318"
                     /pseudo
     CDS             852..1467
                     /gene="tnpA"
                     /locus_tag="OQN02_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1795..2877
                     /gene="ompC"
                     /locus_tag="OQN02_002319"
     CDS             1795..2877
                     /gene="ompC"
                     /locus_tag="OQN02_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1643088.1"
                     /translation="MEKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3366..4164)
                     /locus_tag="OQN02_002320"
                     /pseudo
     CDS             complement(3366..4164)
                     /locus_tag="OQN02_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4174..4725)
                     /gene="ybcL"
                     /locus_tag="OQN02_002321"
     CDS             complement(4174..4725)
                     /gene="ybcL"
                     /locus_tag="OQN02_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS1643089.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4894..5226)
                     /gene="emrE"
                     /locus_tag="OQN02_002322"
     CDS             complement(4894..5226)
                     /gene="emrE"
                     /locus_tag="OQN02_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS1643090.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5571..5885)
                     /gene="fliE"
                     /locus_tag="OQN02_002323"
     CDS             complement(5571..5885)
                     /gene="fliE"
                     /locus_tag="OQN02_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1643091.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            6099..7764
                     /gene="fliF"
                     /locus_tag="OQN02_002324"
                     /pseudo
     CDS             6099..7764
                     /gene="fliF"
                     /locus_tag="OQN02_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7757..8752
                     /gene="fliG"
                     /locus_tag="OQN02_002325"
     CDS             7757..8752
                     /gene="fliG"
                     /locus_tag="OQN02_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1643092.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8745..9431
                     /gene="fliH"
                     /locus_tag="OQN02_002326"
     CDS             8745..9431
                     /gene="fliH"
                     /locus_tag="OQN02_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1643093.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9431..10804
                     /gene="fliI"
                     /locus_tag="OQN02_002327"
     CDS             9431..10804
                     /gene="fliI"
                     /locus_tag="OQN02_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS1643094.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10826..11167
                     /gene="fliJ"
                     /locus_tag="OQN02_002328"
                     /pseudo
     CDS             10826..11167
                     /gene="fliJ"
                     /locus_tag="OQN02_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11164..12291
                     /gene="fliK"
                     /locus_tag="OQN02_002329"
     CDS             11164..12291
                     /gene="fliK"
                     /locus_tag="OQN02_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1643095.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12396..12860
                     /gene="fliL"
                     /locus_tag="OQN02_002330"
     CDS             12396..12860
                     /gene="fliL"
                     /locus_tag="OQN02_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1643096.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12865..13869
                     /gene="fliM"
                     /locus_tag="OQN02_002331"
     CDS             12865..13869
                     /gene="fliM"
                     /locus_tag="OQN02_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1643097.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13866..14279
                     /gene="fliN"
                     /locus_tag="OQN02_002332"
     CDS             13866..14279
                     /gene="fliN"
                     /locus_tag="OQN02_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1643098.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14282..14647
                     /gene="fliO"
                     /locus_tag="OQN02_002333"
     CDS             14282..14647
                     /gene="fliO"
                     /locus_tag="OQN02_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1643099.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14647..15384
                     /gene="fliP"
                     /locus_tag="OQN02_002334"
     CDS             14647..15384
                     /gene="fliP"
                     /locus_tag="OQN02_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCS1643100.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15394..15663
                     /gene="fliQ"
                     /locus_tag="OQN02_002335"
     CDS             15394..15663
                     /gene="fliQ"
                     /locus_tag="OQN02_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1643101.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15672..16457
                     /gene="fliR"
                     /locus_tag="OQN02_002336"
     CDS             15672..16457
                     /gene="fliR"
                     /locus_tag="OQN02_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1643102.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16742..17365
                     /gene="rcsA"
                     /locus_tag="OQN02_002337"
     CDS             16742..17365
                     /gene="rcsA"
                     /locus_tag="OQN02_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1643103.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17409..17597)
                     /gene="dsrB"
                     /locus_tag="OQN02_002338"
     CDS             complement(17409..17597)
                     /gene="dsrB"
                     /locus_tag="OQN02_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1643104.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17760..17987
                     /gene="yodD"
                     /locus_tag="OQN02_002339"
     CDS             17760..17987
                     /gene="yodD"
                     /locus_tag="OQN02_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS1643105.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18285..19100
                     /locus_tag="OQN02_002340"
                     /pseudo
     CDS             18285..19100
                     /locus_tag="OQN02_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(19097..20791)
                     /gene="yedQ"
                     /locus_tag="OQN02_002341"
                     /pseudo
     CDS             complement(19097..20791)
                     /gene="yedQ"
                     /locus_tag="OQN02_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20962..21144)
                     /locus_tag="OQN02_002342"
     CDS             complement(20962..21144)
                     /locus_tag="OQN02_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1643106.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21223..22140)
                     /locus_tag="OQN02_002343"
     CDS             complement(21223..22140)
                     /locus_tag="OQN02_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1643107.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22313..23233
                     /gene="yedA"
                     /locus_tag="OQN02_002344"
     CDS             22313..23233
                     /gene="yedA"
                     /locus_tag="OQN02_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCS1643108.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23222..23692)
                     /gene="vsr"
                     /locus_tag="OQN02_002345"
     CDS             complement(23222..23692)
                     /gene="vsr"
                     /locus_tag="OQN02_002345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1643109.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23673..25091)
                     /gene="dcm"
                     /locus_tag="OQN02_002346"
     CDS             complement(23673..25091)
                     /gene="dcm"
                     /locus_tag="OQN02_002346"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1643110.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25158..25698)
                     /locus_tag="OQN02_002347"
                     /pseudo
     CDS             complement(25158..25698)
                     /locus_tag="OQN02_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25738..>25973)
                     /locus_tag="OQN02_002348"
     CDS             complement(25738..>25973)
                     /locus_tag="OQN02_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643111.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6535 a         6651 c         6399 g         6388 t
ORIGIN      
        1 cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga
       61 tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga
      121 ccttgcggct taactgtcgc tgaagtctcg ccagcttctt ctggtttttc tgaaaactgt
      181 ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg ccagcatcca
      241 gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct gcctgaatac
      301 tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct
      361 gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg ctgttttcct
      421 gagcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc tttttgaatc
      481 aggggaacgc cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg tctttcagtg
      541 actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg gcatttttcc
      601 actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg gccttatgat
      661 tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct gcgaagcgcc
      721 tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc tgtagtcgtt
      781 tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc ggaaggcact
      841 gtgttttcct gatgcatgct caccgggtct ttgttaccaa gtaccgacgc cagatttttg
      901 accatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag
      961 ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tctgttaatt aactatcccc
     1021 ccaaactggc gatatccagt ctggtaaaca aacagcctca aaggcgtatc gggtaggtta
     1081 ctgcgacggg atcgaccaga tattgctgcc aggtattacc gcaaagatgt tttgtggcgt
     1141 cccggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg ccaatacatt
     1201 gaacaacagc aaacacctgg tcagatgaaa aaacgagcct tatatccccg ccctgaagga
     1261 cgggctttta cggtgcactg gataagcctg gagattatct acacctgcat ggacatcatt
     1321 gtactatgcc ccgtacatgc cccatcacat cctcaacatc aaagtattag agccttgcaa
     1381 accattatgc gtctcagttt gtctcatcta tcttacgcaa aaaaaataga actaatatca
     1441 agcggcgcta tattgcgtat taaataaaac cacctcaatt acattaataa atctaaataa
     1501 atatcatgca aataatttcc atggttattt ttaggctatt taaattaaaa aaataaatta
     1561 tgagtcaatc ataaataaca acctttaata taatttgact accaaattta tattcgttca
     1621 caaaatagtc atgcattgtg tgcaatagaa acagttcaga taaaaatagg gatctacttc
     1681 acaatttaaa tgagaaacta aaacttacat cttgaaataa tctcattgat tggatgaata
     1741 tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct cacgatggaa
     1801 aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat gtctgctcag
     1861 gcagcagaga tttacaataa agacagtaac aagctggatc tgtacggaaa agttaatgct
     1921 aagcactact tctcttctaa cgatgcagat gatggcgaca caacttatgt tcgtctgggc
     1981 ttcaaaggcg aaactcaaat caacgatcaa ctgactggtt tcggtcagtg ggaatatgaa
     2041 ttcaaaggca accgtgctga atctcaaggt tcttccaaag ataaaacccg tcttgccttc
     2101 gctggcctga aattcggtga ctacggctcc atcgattatg gccgtaacta cggtgtagca
     2161 tacgatatcg gtgcatggac cgacgttctg ccagaattcg gtggcgatac ctggacccaa
     2221 acagatgtgt tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa caacgacttc
     2281 tttggtctgg ttgatggtct gaactttgct gctcagtatc agggtaaaaa tgaccgcact
     2341 gacgtaactg aagctaacgg tgatggtttc ggtttctcca ctacttatga gtatgaagga
     2401 ttcggtgtag gtgcaaccta tgctaaatct gaccgcacta atgatcaggt tatctacggt
     2461 aacaacagcc tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg tctgaaatat
     2521 gatgcgaaca acatctatct ggctaccacc tattctgaaa cccagaacat gactgttttt
     2581 ggtaataacc atattgccaa caaagcacaa aacttcgaag tagttgcaca atatcagttc
     2641 gacttcggtc tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga cttgggtgcg
     2701 tggggtgatc aggacctgat tgaatatatt gatgtaggtg caacctatta cttcaacaaa
     2761 aatatgtcca cttttgttga ttacaaaatc aacctgattg ataagagcga tttcacgaaa
     2821 gcatctggcg ttgctaccga tgatatcgtt gctgtaggat tggtttacca gttctaatct
     2881 gattactcaa agatatgttg tgggaggctt tgcctcccca acatataagt ggcgctctca
     2941 agccactttc cttagaagca ctatcttgct tctgactata taaattaccc tttattttct
     3001 gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt atcaattaga
     3061 tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca tccctttaat
     3121 tatcccctca acctcttgta aatttcccac tcttgagctc cacatagccc aaacgccgga
     3181 tgcaaaaaaa atcacacgtg catctctgaa attgtgatgg aggaagtgag tatcaattat
     3241 atgtgcttat aaacgttccc gattggcaca gaagtcaccg gattgttcag gctccaatag
     3301 gttgagtatg tcgtatttat tttccaaaat aaaaatcgat gctgttgctc cttaataaaa
     3361 tacttttact ctctggacgc ttttcttagc tctcttggtg ttatattaaa acgattatgg
     3421 aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat ctcgctgatg
     3481 ggttttactg tcgtttgtat agcaggacaa cgcattattt aacctcacgt cgtccaatat
     3541 actttgaaaa cttattccct cgcttgccag acggcgatgc aatgtggaaa cagaaatgta
     3601 gagatatctc gctaccttgt ttgctgtcca ttttgttcta ggttcggata gcattaggtt
     3661 ataacaacga cttattaatg attgtttact atctgataaa agtaaacaat taacatgatg
     3721 tgctcctaat gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg ttgagaagcc
     3781 tcgggaaata gatgttttta gttgctccca acaatatatt aactcggggt tctgaggtaa
     3841 gcagaaacct gtcgtgttac gaatttgatc agttatcgca taaaattttt gaaacttttc
     3901 aattagatca atgggataat aaagcatttc tgcaagataa agacctgctt ctggataatt
     3961 cacaatataa aattcatatc cacagggaaa taatattatc tgattattat caacagttag
     4021 actatgcgtc tcccaattga taactttttt tccctgacgg atacgacata aagctggcat
     4081 aagaggctta accctgtgaa tctcatgatg tttatgcatc cgtatttctt caatctttag
     4141 gttagactta tctcttgcca gcatactctt accctatttt atctcataaa ctggtgttat
     4201 ctcagcggtt gcaattttat tggcattaag catataacca actaacgctc cgctggaatt
     4261 agaatccaca ggaatcttat cagtttttag agcccatact ttaaactggt aatgatgggg
     4321 tttatctcct ttgggaggac atgctccacc aaatcctgca taaccaaaat catttcggcc
     4381 ttgaacagca ccagtcggaa gttttgtacc atcacgtctt cctgcatcag tgggcaaata
     4441 tgttacagtt gctggaatat tagcaacagt ccagtgccac catccactgc ctgtaggagc
     4501 atctggatca tacacagtta cggcaaagct tttggtgcct tcaggagcac cagaccaggt
     4561 taatgagggc gatgtattac caccttcaca cccaaatcca gaaaatacat gagacgttgt
     4621 aagttgctct cctgttttta tttcattact agtgacctga aatgctgcgg cctgcgcaga
     4681 aaatgttatg aatgccaata cacttgaaac gataagtttt ttcataaaaa cctcattgtt
     4741 gtgacctatc gttattttat ttgatgtgcc ttgatctcat tatgaataaa ggcgcaatga
     4801 tcgcgcaaat gcaatcataa ttataaaggt gccccgaaca ttagccataa tatgcacaaa
     4861 tcagattcct gttatctcaa ggagaattat tttttaatgc ggtgcacttc gtgacaacaa
     4921 gttaatgact aacacaccgg cacaaatcaa catcatgcct atgatagctg gcaggtccag
     4981 tcgttgacca aaaaatcccc aggaaagcaa actaatcaga acgataccga ctcctgacca
     5041 gatagcataa gcaatccctg taggtatata agccagcgtc tgagctaata accagaatga
     5101 tgcacaatag caaataattg tgccaacaga gggccataac cgtgtaaacc cctctgaaaa
     5161 cttcattaag gttgtaccaa tgacctctgc aagtattgcg ccaccaagat aaatataagg
     5221 gttcatagca tattctttcc tgttcatact ggagagaatt gtactacagt ttgaacacaa
     5281 ctcaccgttt tcatcctggt gtatgtgtat ccaaggggct ctggcgtggc aagagataaa
     5341 ggcaggaaca ataatgatca gaaggaaccc caggctaacg aatgagttca ctcttcctgg
     5401 gagcaaagcg aagacaaaac atgttatcgc gtttacggat cgtggatggc agaaaacaac
     5461 caggatcagg tatccatttt aaaccagaaa ttgagtgagt ttgccccatc catgccccac
     5521 gctgtgggat cggatataat taaacaagca tagtcaacag gttacacaac ctacacctgc
     5581 atggacatca cttcctgata cgccgccacc agcttattac gcacctgaat ccccatttgc
     5641 atagaaactg aggctttttg catatcggtc atcacatcgt ttagcgccac gccgggttca
     5701 ccgagggtga atttttctgc ctgcgtgcgg gcgactgttt gtgtatcgct tatgcgatcg
     5761 agcgcggcgt gcagctgccc ggcaaaacta atggtcggtt gcggcagtga ttcctgcgca
     5821 cacgcactca tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc ctgtatcgct
     5881 gacattttcg tctcccggat aatttctggt aacaaagcct accagtaagt caataagaca
     5941 aaggcgctaa atagcaacaa aaaaactggt ttattggcgg atagaaaaaa cgaaagcaca
     6001 aataatggga gcgtcaattt ttcgagtttg ctgacccgag agtgagtctt gttccacttt
     6061 gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga
     6121 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgtta
     6181 ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
     6241 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga
     6301 cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgag gttccggcag
     6361 ataaagttca cgaactgcgc ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg
     6421 tcggtttcga actgctcgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga
     6481 attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa
     6541 aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat
     6601 ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa
     6661 ttagcgctat tgtacatctg gtttccagcg cggttgctgg tctgccgcct ggaaacgtca
     6721 cgctggtgga tcagggcggg catctgttaa cccagtccaa taccagcggg cgcgatctta
     6781 atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcag cgtattgaag
     6841 cgatcctgtc acctattgtt ggtaacggta atattcacgc ctaggtcacg gcgcaactgg
     6901 acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcatg
     6961 cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg
     7021 gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc
     7081 ctccgacaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat agtgggccgc
     7141 gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca
     7201 aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct
     7261 tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt gaagctctga
     7321 cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc
     7381 cgttcaatag cagtgacgaa agcggcggag cactgccgtt ttggcaacag caagtgttta
     7441 tcgatcagtt gctggctgcc ggtcgctggt tgctggttgc tggtactgct ggtggcgtgg
     7501 ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc gcgccgaggc ggtgaagact
     7561 gtgcagcaac aggcgcaggc ccacgaggaa gtggaagatg cggtggaagt ccgtctgagc
     7621 aaagacgaac aactccaaca acggcgcgct aaccaacgac tgggggcaga agtcatgagc
     7681 cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt cattcgccag
     7741 tggataaata acgatcatga gtaacctgac aggcaccgat aaaagcgtca tcctgctgat
     7801 gaccattggc gaagaccggg cggcagaggt gttcaagcac ctctcccagc gcgaagtgca
     7861 aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc taaccgatgt
     7921 gctggcggag tttgagcaag aagctgaaca gtttgctgca ctgaatatca acgccaacga
     7981 ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa cgtgccgcca gcctgctgga
     8041 agatattctc gaaacccgcg ataccgccag cggtattgaa acgctcaact ttatggagcc
     8101 gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa attatcgcca ccattctggt
     8161 gcatctgaag cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca
     8221 cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc tggccgaact
     8281 gaccgaagta ctgaatggct tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg
     8341 cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg cagcaggaag aagccgttat
     8401 taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa atcatcgacg agatgttcct
     8461 gttcgagaat ctggtggatg tcgatgatcg cagcattcag cgtctgttgc aggaagtgga
     8521 ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag ccactgcgcg agaaattcct
     8581 gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca accgtggtcc
     8641 ggtgcgtctg tcgcaggtcg aaaacgaaca gaaagcgatt ctgctgattg tgcgccgtct
     8701 tgccgaaact ggcgagatgg tgattggcag cggcgaggat acctatgtct gataatctgc
     8761 cgtggaaaac ctggacgccg gacgatctcg cgccaccacc ggcagagttt gtgccaatgg
     8821 tcgagtcgga agaaaccatt attgaagagg ccgaacccag ccttgagcag caactggcgc
     8881 aactgcaaat gcaggcccac gagcagggtt atcaggcggg gattgccgaa ggtcgccagc
     8941 aaggtcacga acagggctat caggaaggac tggcccaggg gctggaacaa ggtctggcag
     9001 aggcgaaggc tcaacaagcg ccaattcatg cccggatgca gcaactggtc agcgaatttc
     9061 aaactaccct tgatgcgctt gatagtgtga tagcttcgcg cctgatgcag atggcgctgg
     9121 aggcagcacg tcaggtgatc ggtcagacac caacggtgga taactcggca ctgatcaaac
     9181 agatccaaca gttgttgcag caagaaccgt tattcagcgg gaaaccacag ctgcgcgtac
     9241 acccggatga tctgcaacgt gtggatgata tgctcggcgc aactttaagt ttgcatggct
     9301 ggcgcttgcg gggcgatccc accctccacc ctggcggatg taaagtctcc gccgatgaag
     9361 gcgatctcga cgccagtgtc gccactcgct ggcaagaact ctgccgtctg gcagcaccag
     9421 gagtggtgta atgaccacgc gcctgactcg ctggctaacc acgctggata actttgaagc
     9481 caaaatggcg cagttgcctg cggtacgtcg ctacgggcga ttaacccgcg ctaccgggct
     9541 ggtgctggaa gccaccggat tacaattgcc gctcggcgca acctgtgtca ttgagcgcca
     9601 gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg gtcaacggct
     9661 gtttttaatg ccgctggagg aagtggaagg tgtcctgccc ggcgcgcgtg tatatgccaa
     9721 aaacatttcg gcagaagggc tgcaaagcgg caagcagttg ccgctcggtc cggcgttatt
     9781 aggtcgtgtt ctggacggta gcggtaaacc gctcgatggc ctgcccttcc ccgatacgac
     9841 ggaaaccggt gcgctgatta ccccgccatt taacccgttg caacgtacac cgattgaaca
     9901 tgtgctggac accggcgtgc gcccaatcaa tgccctgctt accgtcggac gcgggcagcg
     9961 tatggggctg tttgccgggt cgggcgttgg taaaagtgtg ctgctgggga tgatggcacg
    10021 ctacacccgc gccgatgtca ttgtcgtggg tttgattggt gaacgtgggc gcgaagtaaa
    10081 agattttatt gagaatatcc tcggtgccga aggacgtgca cgctcagtgg tgattgccgc
    10141 tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc gcctatgcca cgcgcattgc
    10201 cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt atggactccc tcacccgcta
    10261 cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa ccacctgcca ctaaaggtta
    10321 tccaccgtcg gtgtttgcca aattaccggc actggtcgag cgtgccggta atggcattag
    10381 cggcggcggc tcgattaccg cgttttatac cgtgctcacc gaaggcgatg accagcagga
    10441 ccccattgct gactccgcgc gggcaatcct cgacggtcac attgtgctgt ctcgccgact
    10501 ggcggaagcc gggcactatc cggctatcga tattgaagcg tcgatcagtc gcgcaatgac
    10561 ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc ttcaaacagc tgttgtcgag
    10621 ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat gccaaaggca gcgatccgat
    10681 gctcgataaa gccatcgccc tgtggccgca gctggagggc tatttgcaac aaggcatttt
    10741 tgaacgcgcg gactgggaag cgtctctcca ggggctggag cgtattttcc cgacagtgtc
    10801 ataacccagg agataacggc agatggcgga agaacagctc aaaatgttga tcgattatca
    10861 gaatgaatat cgcaataacc tcaatagcga tatgagtgcc gggatgacca gcaaccgctg
    10921 gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc atcgccagca
    10981 acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag aaaagaaaca
    11041 acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac tgcttgcaga
    11101 aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca tgaggaaacc
    11161 tgaatgattc gcttagcgcc cttgattacc gccgacgttg acaccaccac attgcctggc
    11221 ggcaaagcca gcgatgctgc acaagatttt ctcacgttgt tgagcgaagc attagcaggc
    11281 gagacaacaa ctgacaaagc ggccccccag ttgctggtag caacagataa gcccacgaca
    11341 aaaggcgagc cgctggtcag cgatattctt gccgatgcgc aacaagcgga tttactgatc
    11401 cctgtggatg aaacacttcc tgtcatcaac gacgaacaat ccacatcaac accgttaacc
    11461 accgctcaga cgatgacgtt ggctgcggtg gctgacaaaa atacgacaaa agacgaaaaa
    11521 gcggatgatc ttaatgaaga cgtcaccgca agtctgagcg ccctttttgc gatgttgccg
    11581 ggttttgaca atacgcccaa agtgactgat gcaccgtcaa ccgtgttacc ggcagagaaa
    11641 ccaacgctct tcacaaaact gacttctgcg caactcacta cagcacagcc tgatgacgcc
    11701 cccggcacac cagctcagcc attaacaccg ctggtagcag aagcccagag taaagcggaa
    11761 gtcatcagca cgccttcgcc ggtgaccgct gacgccagcc cgctaatcac tccacaccag
    11821 acacagccac tgcccaccgt cgccgcgcct gttttgagtg caccgctggg ttctcacgaa
    11881 tggcaacaat cattgagcca gcatatttcg ctgttcaccc gccaggggca acaaagtgca
    11941 gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa tttccctcaa agtggatgat
    12001 aaccaggcgc agatccagat gatttcaccg catcagcacg tacgcgccgc cctggaagca
    12061 gcgctgccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg gcaaagcaac
    12121 attagtggcg aaagctttag tggtcagcag caggccgctt cccagcaaca gcaaagccaa
    12181 cgcacagcaa accatgaacc tctggcgggg gaagacgacg atacgcttcc ggttcccgtc
    12241 tctttacaag ggcgtgtaac aggtaacagc ggcgttgata ttttcgccta acgtcagagg
    12301 tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag gataattagc
    12361 cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg ataagcaaga
    12421 aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg gcctgtgcca
    12481 gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac
    12541 gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt tacggtcaat ttgggcgatg
    12601 cggatcgcgt gctttatatc ggcataaccc tgcgactgaa agatgaagca acccgctcgc
    12661 ggctgagtga gtatttgccg gaagtccgta gtcgcttgct gttactgttt tcacgtcagg
    12721 atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag attaaaacca
    12781 cactttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac gtgctgtata
    12841 ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc tgaaattgat
    12901 gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtgt tagcggcgaa
    12961 agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga acgtttgcag
    13021 gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct gttcaacctg
    13081 ctgcgccgta gcccggacat taccgtcggg gccatccgca ttcagccgta ccatgaattt
    13141 gcccgcaacc tgccggtgcc gaccaacctg aacctaatcc acctgaaacc gctacgcggc
    13201 acagggctgg tagtgttctc gccgagtctg gtgtttatcg ccgtggataa cctgtttggc
    13261 ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac tgaacagcgc
    13321 gtcatcaacc gtatgttgaa gctggcgctg gaaggctata gcgacgcctg gaaagcgatt
    13381 aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tggaatttac caatatcacc
    13441 acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg caacctgacc
    13501 ggcgaattta atatctgcct gccattcagc atgatcgagc cgctacggga attgttggtt
    13561 aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc
    13621 caggtgcagc attcacagct ggaactggtg gccaactttg ccgatatctc gctacgcctg
    13681 tcgcagattt taaaactgaa acccggcgac gtcctgccga tagaaaaacc cgatcgcatc
    13741 atcgcccatg ttgacggcgt cccggttctg accagtcagt acggtactct caacggtcag
    13801 tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa cgaggaacag
    13861 cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg gacgatctgt
    13921 gggctgaagc gttgagcgag caaaaatcaa ccagcgaaaa aagcgctgcc gacgcggtgt
    13981 tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac ctgattatgg
    14041 atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt
    14101 tgcgtctgac gcaagggtca gtcgtggcgc tggacggtct ggcgggcgaa ccgctggata
    14161 ttctgatcaa cggttattta atcgcccagg gcgaagtggt ggttgttgcc gataaatatg
    14221 gcgtgcggat caccgatatc attaccccgt ccgagcggat gcgccgcctg agccgttagt
    14281 gatgaataac catgctactg tgcaatcttc cgcgccggtt tctgctgagc cactgctgca
    14341 ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct ggctggtaaa
    14401 acggctggga tttgccccta aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc
    14461 ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg gaagatgcac ggctggtgct
    14521 cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag caccaacgga
    14581 agagataccg cagaccgatt ttcagtcggt catgaaaaat ttgcttaagc gtggcgggag
    14641 atcctgatgc gtcgtttgtt ttctgtcgca ccggtccttc tctggctgat tacgcccctc
    14701 gccttcgcgc aactgccggg tatcaccagc cagccgctac ctggcggtgg acaaagctgg
    14761 tcgctcccgg tgcagacgct ggtgttcatc acctcgttga cgttcattcc agcgatttta
    14821 ctgatgatga ccagtttcac ccgcatcatt attgtttttg gtttattgcg taacgcgctg
    14881 ggaacgccct ccgcgccacc taaccaggta ttgctggggc tggcactgtt tttgaccttt
    14941 tttattatgt cgccagtgat caacaaaatt tatgtcgatg cgtaccagcc attcagcgaa
    15001 gagaaaatct caatgcagga ggcgctggaa aaaggggcgc agccgctgcg tgagtttatg
    15061 ctgcgtcaga cccgcgaggc agatttaggg ttgtttgcca gactggcgaa taccggcccg
    15121 ttgcaggggc ctgaagctgt gccgatgcgc attttgctcc cggcctacgt gaccagcgag
    15181 ttgaaaaccg catttcagat aggcttcacg attttcatcc cttttttgat tatcgacctg
    15241 gtgattgcca gcgtgttgat ggcattgggg atgatgatgg ttccccccgc caccattgct
    15301 ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct ggtcggttcg
    15361 ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt catgatgatg
    15421 ggaactgaag cgatgaaagt cgcgctggca ctggcagccc cgctgttgct ggtagcgctg
    15481 gtcacgggcc ttatcatcag tattttgcag gcagccacgc agattaacga aatgacgctg
    15541 tcgtttattc cgaaaatcat cgccgtattt atcgccatca ttattgccgg accgtggatg
    15601 ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta tatcatcggg
    15661 tagcccgtac aatgatgcag gagacaagcg atcaatggct ttcctggtta agcctgtact
    15721 tctggccgtt actgcgcgtg ctggcgctca tttctaccgc gccaattctg agcgaacgca
    15781 gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgcc attgccccat
    15841 cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg gccgtacagc
    15901 agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc gctgtgcgaa
    15961 ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt tgcgacattt gtcgatcctg
    16021 gcagccatct caatatgccc gttttagcgc gcatcatgga tatgctggca ttactgctgt
    16081 tcctgacatt taacggtcat ttatggttga tttcactgct ggtcgatacc tttcacaccc
    16141 tgccgattgg tagcgaaccg ttgaacagca atgcgtttct ggcacccacc aaagcaggga
    16201 gtttgatttt ccttaacggg ctgatgctgg cgttaccgct cattactctg ttgctgaccc
    16261 tgaatctggc attaggttta ctgaatcgta tggccccgca attatccatt tttgttattg
    16321 gatttccatt aactctgact gtcggcatct ctttaatggc ggcattaatg ccgttaattg
    16381 cgcctttttg cgaacattta ttcagtgaaa tttttaattt gctggctgat attattagtg
    16441 aattaccatt aatataattc cgtaacgttt atcatgctac cctaaggatt atccgaaaaa
    16501 taatatctgc gaacatcttc caggatactc ctgtagcgaa atatttgttt taagctcact
    16561 cacatatcgc aacatttact ttaagacaat tccaggcaaa ttatacaaca ctttacggga
    16621 tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag gtgacccatg ttgttccgtt
    16681 tagtcatgat gaaatattca ggtaagggga attatcgtta cgcattgagt gagggtatgc
    16741 catgtcaacg attattatgg atttatgtag ttacacccga ctaggtttaa ccgggtatct
    16801 gttgagtaga ggggttaaaa aaagagaaat caacgacatt gaaaccgttg atgaccttgc
    16861 catagcttgt gattcacagc gtccttcagt ggtgtttatt aatgaggact gtttcatcca
    16921 cgatgcttct aacagtcagc gcatcaagca catcattaat caacatccca atacgttatt
    16981 tatcgttttt atggcaattg ccaatgttca ttttgatgaa tatctattgg tcagaaaaaa
    17041 tttattgatc agttctaaat cgattaaacc ggaatctctc gacgatatcc ttggcaatat
    17101 tctgaaaaaa gagacaacga taacctcgtt tttaaatatg ccgacgttat cattgagccg
    17161 aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag ggaaccattc aaatctccga
    17221 ccaaatgaat atcaaagcca agaccgtttc atcgcataaa ggtaatatta aacgtaagat
    17281 caaaacgcat aataaacagg ttatctacca tgtcgtccga ctgacggata atgtgactaa
    17341 tggtattttt gtcaacatgc gctaacacat tctgactggt gatttcccac cagtcaggct
    17401 gaataagatt actctgcttt ctcaacaaag ataccgtcct gatgccctgc ttcattaaag
    17461 aaccagatgc cgagcgggta gtcttccagc gcaaccaggt acattgtgcc ttcactaaac
    17521 tcctcaactg ccagtaccac tccaggacga cgcggaccgc catccgtttt gactgttacc
    17581 cgatcattca ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga acaatttcgt
    17641 ttttctggca gcgccaagcg cgcgcgagtg ctgattttct cgacggtcta tacttaagag
    17701 atgctaggcg gacttaacga ctggcggcaa caacagagta acggttgcga ggaaagatga
    17761 tgaaaaccgc aaaagtgtac agcgataccg caaaacgtga ggtcagcgtc gatgtcgatg
    17821 ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc cagaacgatt
    17881 ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg gcggatgcct
    17941 tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatt cacaataaaa
    18001 aaatcccgac cctgagggtg tcgggatgaa acttgcttaa gcaagaagca cttaaaaaat
    18061 tcgttacacc aggaaatctg atgtgttcat caccttatct gcaatttttt tcgctgacaa
    18121 gaaaatattc gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg acgcgcgtca
    18181 ccccttggtg gctctggtgt caacgggatc gctcccgaaa aaatcatctt tgattatttg
    18241 tgctgttttg agaatcatcc tggtaagggg agtactgcgc gaccatgttt tcaattcaac
    18301 aaccactact ggtttttagc gatcttgatg gcaccctgct ggacagtcat agttatgact
    18361 ggcaaccggc agccccctgg ctcagccgtt tacacgaagc caatattccc gtcattctct
    18421 gtagcagtaa aactttagcg gaaatgctgt acttgcaaaa aatgttgggg ctacaaggct
    18481 taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg caggatatag
    18541 acggttttcc acgcatcatc tcaggtatta gccatggcga aatctgccag gttttaaata
    18601 cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac gatgcaacca
    18661 ttgccgaatg gacgggatta agccgtagct aggcggcgct gacgcagctg catgaggcgt
    18721 cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttatc gctcgtctga
    18781 acgaactggg cttgcagttt atgcagggtg cgcgcttctg gcacgtactg gatgcctctg
    18841 ccggaaaaga tcaggctgcc aattggatta tcgcgaccta tcaacaattg tcaggcagac
    18901 gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcctttactg gaggtaatgg
    18961 attacgcggt gattgtgaaa gggctaaacc gtgaaggggt acatctgcat gatgaggatc
    19021 cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa gggctggacc
    19081 attttttctc cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc ccggcctgtt
    19141 ttgcgaggta aagccgacgg tcggccaatg actggagttg ttcaaaatca taatcaccgg
    19201 tttcctcgct gctacttacc cccagcgagg cactgatgcg tatcgttgta ctcttggcga
    19261 tcagcatctc tttttcattt aacttcaggc gaatacgttc tgcgacctcc gcagcctgcg
    19321 tcagattcgc gcctggcaga atcacacaaa actcctcacc accgacccgc ccggcaacgt
    19381 cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca cggtcgcccg
    19441 cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc tgaatgacag
    19501 aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg acgggctttt tcgaacagtg
    19561 cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc aacgagcttt
    19621 gcagaacata catgttgctg accatccggc gaatcacata ccaggagagg agtaacatgg
    19681 tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa tcgccgcgca
    19741 cgccttcgct tagcgtatgg acgcgcgcca gcacaccgtc gaaatggtcc agacgttccc
    19801 agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc attgcctgcg
    19861 ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca ggattggaag
    19921 aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg tttttatcga
    19981 tggcgtttct taaaaattgc tgcatagtac gcacgggaat actcatcccc agcacgccat
    20041 accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca gtattgctgg
    20101 cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct cgttgcgaat
    20161 ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgttggtaca ttgcgcgtaa
    20221 acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga gacatacatc gcctgttcaa
    20281 ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc aacttccagt gcagcggtaa
    20341 tttcattgtc gaggctttca ttttcgcgag acaggaaatt cccctcgctg actaatgcat
    20401 ccgatacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc caggcgtgct
    20461 cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag agaggtgaaa tccagtggcg
    20521 ccacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg ttatactgca
    20581 aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctagctg gagatatagg
    20641 catcttccag caccaccact tcacgccagg tgagcaaggt ggaaaaaagc aataccacga
    20701 taaagcagag attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt ttcaaccagc
    20761 tctggttttc catttttgtc tcgtgctgca ccctgacgac tcccaacctg cttttttatg
    20821 attctggcat aacccggcaa tagcgtaccg caaaacagcg ataattttat aatggtggca
    20881 tgataagacg cgactatcat tcattgcagg cattgggaca ccaggccgga tgtgatccgg
    20941 cctggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct cccccggaac
    21001 aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc aacgacactc
    21061 aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt taaccgtaac
    21121 ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac ttgttcacct
    21181 tagttgaaga tggcgaattt tgcataatag ccagtgcgag tattaatgtg cctgaccgcg
    21241 cattttcgct actgctttca ctcccagcac cacgatgccg ccgatgataa atccaagaat
    21301 cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc cggcgatatg
    21361 ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc cgacgaggaa
    21421 catcgccagc gtgccgacaa tcgataatgc tttcatcagc caaggcgcga taatcaataa
    21481 ccctttacct aatgcctgca tcagcgcgct agatttttcc gccagccaat accctaggtc
    21541 atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta ccagcgcgat
    21601 gcctgaaagc accagcacct gattaagcaa cggcgcctct gccacaatcc ccagcgtgat
    21661 ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc gcccctttaa ttttgtcctt
    21721 ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctggc tctgcgctgg
    21781 atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc cttcaaagca
    21841 gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc cacgggataa acgcactgat
    21901 gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg ccacgcccca
    21961 gaccacggga agttcccggt tggcccgcac gcctgaaact tgttgcgcat tgagcgataa
    22021 atcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca cagagatatc
    22081 gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg ccaaaagcat
    22141 tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca gctctgatac
    22201 ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct cgccagcaga
    22261 atgattttca cgtttactat gaaccacctt tttatttccg ccgtgaggga ttatgcgttt
    22321 ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt ggggctcaac
    22381 ctattttgtc attcggattg gcgtgggaag ctggcctccg ttaatgatgg cgggcgttcg
    22441 attccaggca gccggtattt tattgctggc atttttgcta ctgcgcggac acaaacttcc
    22501 cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg ctgtcggtaa
    22561 tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg ccgtagtgat
    22621 tgcaaccgtg cccctcttta ccctgtgctt cagtcgcctg tttggcatta aaacgcgcaa
    22681 actggaatgg gtgggtattg ccattgggct tgccggaatc atcatgctca atagcggtgg
    22741 aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga ttagctgggc
    22801 gtttggctca gtttatggct cgcgcattac cttacctgta gggatgatgg cgggtgcgat
    22861 tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg gtgaaaaact
    22921 gacggcgctc ccttccctgt caggcttcct tgcggtcggc tatctggcgc tgtttggttc
    22981 gattatcgcc attaacgctt atatgtattt aatccgtaat gtcagtccgg ctctcgccac
    23041 cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac tgggtggcga
    23101 aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gttttcgcgg tggtactggt
    23161 cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta ttcaggatgc
    23221 atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct tcgccgcaga
    23281 tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc ctacgtaacg
    23341 cacactccca gacaatcagt acgcgccagc cgagctcctg caagcgactg atatcgcggc
    23401 gatcgcgttc aacattttta cctatcttct ccagccagaa ctcagttcga gtcgcaggca
    23461 ctttaaacag atagcagtga tgatgatgcc agaagcagcc gtgggtaaat atcacgcagc
    23521 gatattcatc aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga acgcgaaacg
    23581 caaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta tcacgcgtgg
    23641 caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc atgttgtgcc
    23701 tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt tgccaccgcg
    23761 gcaaagaccg gcacgaccac cgagttgccg aactggcgat aggcctgagt gtccgaaacc
    23821 ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc gcattcacga
    23881 ggcgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaagtc tttctcaccc
    23941 gtggccatat cccaaccgcg atcgattaaa atttccgcgc catctttgta ataacgagca
    24001 gacagcgtac gggtgacgct ttgcggattg ttcggataaa ccattccata accgaagccg
    24061 ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag caccggcgtc
    24121 aggatatatt tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac tcgctgtgca
    24181 gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattcag atcgcgacga
    24241 aaacccacca gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc atcgatgatt
    24301 ttcggatcgt ccggcccgtt atcttctgca tcggcaacgt catagcccag ttcgtccagc
    24361 gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt tttgacgttt
    24421 tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc aaagaacagc
    24481 gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt tttcgatacg
    24541 ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg ttcaggaatg
    24601 tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg actgagggtg
    24661 atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg gttggcttta
    24721 taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc accaatcgat
    24781 tcaaaaccac gacggatgcc gccaattccg gcaaatagat cgataaagcg aaacgcataa
    24841 tgcggatgat gttccggtgg tttgggtaac aacgtttgca gatgggcaaa ctctttctca
    24901 cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc cgcgctccag
    24961 tgattctcac ccacaccatt aagctgcgcc accagcattt taacatcata aatttgcagc
    25021 agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt tactgatata
    25081 ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca taattactgc
    25141 cgtttagggc cacgcgctca gccagcgggt gaaaacgcat ctatcacctt gtgatcgata
    25201 ccttcattct cccccgccag ttcggcaccg agcttcgcca tcaaacagcg ccaagctcaa
    25261 tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc cccagggctt ccagccgatc
    25321 cgcatcctgg acgattttag cttccgttgt taagggggcg atttgcgcgc tgaaactgtg
    25381 agcggcaatg gcatgacaaa cggcctcaat tttctccgcc ggaaattgca cgaactcttc
    25441 acggagcagg cgacgcgtct cttctgccgc caggattgaa gaacgctgcc gttgcggatg
    25501 atttttcgcc aggctcacga tatcgtgaaa ataacaggcg gttaaaatca ccagcatatc
    25561 gacatcgtca tctgccgcca gtttctgtgc cgtagcccag acacggcgaa aatgacacag
    25621 gtcatgcgcc gcgtcctgat gttgatgatg gtttttcaac cagttctcga actgtgcctg
    25681 ccagtgttgt aactccatac gcgctccgtt gccaaagagt cgcaacctta gcagttttta
    25741 ttcatagcgt ctgcaacgtg cgggacgagg tcgggtgtac catgcaatgc cgcctaacaa
    25801 ggcaccaacc agggccataa acagaaatcc gaccaatgct cctaaccact tccccgcggt
    25861 agatcctaaa tcactttcaa atcccgacac gggggcatcg gggatccggc tcatcaccca
    25921 gatgtatcgc gccattgccc agacgaaata gccacaccat gtatagaaag ccc
//
LOCUS       DAJVRL010000058        25725 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVRL010000058 DAJVRL010000000
VERSION     DAJVRL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.058"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQN02_002349"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQN02_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1643112.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQN02_002350"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQN02_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1643113.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQN02_002351"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQN02_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1643114.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQN02_002352"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQN02_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1643115.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQN02_002353"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQN02_002353"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1643116.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQN02_002354"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQN02_002354"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1643117.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQN02_002355"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQN02_002355"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1643118.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OQN02_002356"
     CDS             complement(4598..5320)
                     /locus_tag="OQN02_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1643119.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQN02_002357"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQN02_002357"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1643120.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OQN02_002358"
     CDS             6520..6738
                     /locus_tag="OQN02_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1643121.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQN02_002359"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQN02_002359"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1643122.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OQN02_002360"
     CDS             complement(7472..7672)
                     /locus_tag="OQN02_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1643123.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OQN02_002361"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OQN02_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OQN02_002362"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OQN02_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1643124.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OQN02_002363"
     CDS             10101..12014
                     /locus_tag="OQN02_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1643125.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OQN02_002364"
     CDS             12014..13036
                     /locus_tag="OQN02_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1643126.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OQN02_002365"
     CDS             13030..13248
                     /locus_tag="OQN02_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1643127.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OQN02_002366"
     CDS             13302..14171
                     /locus_tag="OQN02_002366"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1643128.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OQN02_002367"
     CDS             complement(14226..14630)
                     /locus_tag="OQN02_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1643129.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OQN02_002368"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OQN02_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1643130.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OQN02_002369"
     CDS             15615..17705
                     /locus_tag="OQN02_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1643131.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OQN02_002370"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OQN02_002370"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1643132.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OQN02_002371"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OQN02_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1643133.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OQN02_002372"
     CDS             complement(19673..20275)
                     /locus_tag="OQN02_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1643134.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OQN02_002373"
     CDS             complement(20265..20432)
                     /locus_tag="OQN02_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1643135.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OQN02_002374"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OQN02_002374"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1643136.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OQN02_002375"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OQN02_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1643137.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OQN02_002376"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OQN02_002376"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1643138.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OQN02_002377"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OQN02_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1643139.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
BASE COUNT         6295 a         6929 c         6429 g         6072 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag attgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggtaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct ctcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcccgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctcc
//
LOCUS       DAJVRL010000059        24180 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVRL010000059 DAJVRL010000000
VERSION     DAJVRL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.059"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(22..594)
                     /locus_tag="OQN02_002378"
     CDS             complement(22..594)
                     /locus_tag="OQN02_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCS1643140.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OQN02_002379"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OQN02_002379"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1643141.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OQN02_002380"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OQN02_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1643142.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OQN02_002381"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OQN02_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1643143.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OQN02_002382"
     CDS             complement(3556..5337)
                     /locus_tag="OQN02_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1643144.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OQN02_002383"
     CDS             complement(5330..7102)
                     /locus_tag="OQN02_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1643145.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OQN02_002384"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OQN02_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1643146.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OQN02_002385"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OQN02_002385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1643147.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8661..10353
                     /locus_tag="OQN02_002386"
                     /pseudo
     CDS             8661..10353
                     /locus_tag="OQN02_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10418..11113
                     /gene="queC"
                     /locus_tag="OQN02_002387"
     CDS             10418..11113
                     /gene="queC"
                     /locus_tag="OQN02_002387"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1643148.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="OQN02_002388"
     CDS             complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="OQN02_002388"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1643149.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11657..12028)
                     /locus_tag="OQN02_002389"
     CDS             complement(11657..12028)
                     /locus_tag="OQN02_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1643150.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="OQN02_002390"
     CDS             complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="OQN02_002390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1643151.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="OQN02_002391"
     CDS             complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="OQN02_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1643152.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14723..17077)
                     /gene="lon"
                     /locus_tag="OQN02_002392"
     CDS             complement(14723..17077)
                     /gene="lon"
                     /locus_tag="OQN02_002392"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1643153.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="OQN02_002393"
     CDS             complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="OQN02_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1643154.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="OQN02_002394"
     CDS             complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="OQN02_002394"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1643155.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19535..20833)
                     /gene="tig"
                     /locus_tag="OQN02_002395"
     CDS             complement(19535..20833)
                     /gene="tig"
                     /locus_tag="OQN02_002395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1643156.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="OQN02_002396"
     CDS             complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="OQN02_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1643157.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21799..22377
                     /locus_tag="OQN02_002397"
     CDS             21799..22377
                     /locus_tag="OQN02_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1643158.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22421..23896
                     /gene="ampG"
                     /locus_tag="OQN02_002398"
     CDS             22421..23896
                     /gene="ampG"
                     /locus_tag="OQN02_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1643159.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5665 a         6664 c         5827 g         6024 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcattcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 taatattgtt ccgggtttat atgcgattgc tcaaccgtct taaccagtat caacgtctgt
     8701 ggcaaccttc agccgcaaac cgtcaccgtc agcgaactgg ccgaacgctg tttttgcagc
     8761 gaacgccatg ttcgtacgct gttgcgtcag gcacaggagg cgggatggct ggagtggcag
     8821 gcgcagtcag gacgcggaaa gcgcggacaa ttacgctttc tggtcacgcc ggaatcgcta
     8881 cgcaatgcga tgatggaaca ggcactggaa accggaaagc agcaagatgt gctggagctg
     8941 gcgcaactgg ccccaggtga gctgcgcact ctgttacagc catttatggg cggacaatgg
     9001 caaaacgata cacccacgtt gcgtattccc tactatcgcc cgctcgaacc gctacaacca
     9061 ggctttttgc ccggccgtgc cgagcagcat ctcgccgggc agatattttc cggcctgacc
     9121 cgcttcgata ataatactca gcgcccgatt ggcgatttag cgcatcactg ggaaacctct
     9181 actgacaggt tacgctggga cttttatctt cgttcaaccc tacactggca taacggcgat
     9241 gcagtaaaag cctcacactt acaccagcga ttattgatgc tgttacaact gccagcactg
     9301 gatcaattat ttattagcgt gaagcgtatt gaagtcaccc atccgcagtg tctgaccttc
     9361 tttttacatc gccccgatta ctggcttgcg caccggctgg cgagctattg cagccatctg
     9421 gcgcatccgc aattcccact gatcggcact ggtccttttc gcttaacaca attcacagcg
     9481 gagctggtgc gcctggagag ccatgattat taccatttgc gtcatccgct gcttaaagcg
     9541 gttgagtact ggataactcc gccgcttttc gaaaaagatt tgggaaccag ttgtcggcat
     9601 cccgtgcaaa tcaccatcgg caaaccggag gagctgcaac gggtcagcca agtcagtagt
     9661 ggcatcagtt taggtttttg ctatttaacg ttgcgcaaaa gtccccgact ctccctctgg
     9721 caggcgcgaa aagtgatctc cattattcat caatccggtt tattacaaac gttagaagtc
     9781 ggagaaaacc tgatcaccgc cagtcatgca ttactgctag gctggactat tccgcactgg
     9841 caagtaccgg atgaagtcaa actaccgaaa accttgacgc tggtttatca cctaccgata
     9901 gaacttcata ccatggcaga acgcctacag gcgacactgg cagcggaagg ctgtgaactc
     9961 acaattattt ttcataacgc aaaaaactgg gacgacacga ccctactggc acacgcagac
    10021 ctcatgatgg gcgacagatt aattggcgaa gcaccggaat atactctgga gcaatggcta
    10081 cgttgcgatc cactatggcc acatgttttc gacgctccag catatgcaca tctgcaatcg
    10141 acgctggacg cggtgcaagt aatgcctgat gaggaaaacc gatttaatgc cctgaaagcg
    10201 gtttttagcc agttaatggc agacgcgacg ctgacgccgc tgttcaacta tcactatcgc
    10261 attagtgccc ctcccggcgt gaacggtgtg cgactgacac cgcgcggctg gtttgaattt
    10321 accgaagcct ggcttcccgc gccgtcgcaa tgaatagctg gtccgggtta atcgcaggcg
    10381 ttaccataac gcttttattg ttcaagcagg attatctatg aaacgtgctg tcgttgtgtt
    10441 cagtggaggc caggattcca ccacctgtct ggtgcaggca ttacaacaat atgatgaagt
    10501 ccattgcgtg acgttcgatt acggtcagcg acatcgcgca gaaatcgacg tggcacgcga
    10561 actggcgctg aaactgggag cacgcgcgca taaggttctt gatgtaacgc tgctcaacga
    10621 gctggcggtc agcagcctga cacgtgacag cattccggtg cctgattatg aacctgaagc
    10681 cgatggcatc ccgaatacgt ttgtcccagg gcgtaatatt ttgttcctga cgctggcggc
    10741 aatatatgcg tatcaggtaa aagcagaagc cgtaattacc ggcgtctgtg aaacggattt
    10801 ctccggctac ccggattgcc gcgatgagtt tgtgaaagca ctaaaccatg ccgtcagttt
    10861 gggcatggcg aaagatattc gttttgaaac gccgctgatg tggattgata aagcggaaac
    10921 ctgggcgctg gcagattatt acggcaaact ggatttagtc cgtaacgaaa cgttgacctg
    10981 ctataacggc attaaaggcg acggttgcgg tcattgtgcg gcatgtaatt tacgcgccaa
    11041 cggtttgaat cattatctgg ccgataaacc gacggtgatg gcagcgatga agcagaaaac
    11101 cgggttgagg taattattcc gggtgtcgcc ggatgcggct tgagcatccg gcaccacaaa
    11161 acgtttactt aaccatctgc tccagctttt cgcgcaattc cccttccaga gctaatgctt
    11221 tctgcgtttt aagatcaata caaacaaacg taataagcgc atccgctacc acctgccctt
    11281 ccggctccag tgtaatgacc tggtttaaga tgccgctttt accgtttaat tgctgcaact
    11341 gactggtaat agttaacagg tcgcttaata ccgctgggcg acgatagtta atattgatat
    11401 tgaccacgac gaaggcgatg ttatgggccg tcatccactg aaaactgtcg ctattttcca
    11461 ccccatccca gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg acgtgctggt
    11521 aaacgtcgag atgatatcca cgaactttga tttgtgtttg catagcgcaa taaccttacg
    11581 ttgttgttat aagtacagag gtcataactg gtatgacctc ttcagtctgg caaaaaattg
    11641 ccactatgca aattaattac agggttaata ccgccagatt acgttccacc agcgaattgc
    11701 ccatccccgg cacctgcttt agatcctcca ccgttttaaa cggaccgtac tcttcgcgat
    11761 aactgacaat cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg gctagctctt
    11821 ccgcgctggc attattaatg ctgacccggg tgccttcttc gtcactggct ttcgccggta
    11881 ctgctgcttt actttgtgct gcaggagctt ccgctttggt ttctaccgcc gtcggtttcg
    11941 ccacagaagc tgccgccagc gcgctatgag acattccggc acaggccagg gacagggtaa
    12001 tgagcagtgc tttaattccg tgtttcatac tgttttctcc ttgtttgtta acagtggaat
    12061 caccttaacg ggagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg aaaaggccgc
    12121 gaaagcgacc ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgg cttcaggatt
    12181 attgctgttc cagcgcatcg ccaattttga ttttcgcctc tttacgcagg ttactcatca
    12241 gagcttcaaa gacgatttgt gcgttgttct gggtgatacc ctgcaccatc gcttttttct
    12301 gatcttccgg cattgaacct tgtttcactt catccagcgc cagcagaacc acattacctt
    12361 gcatatcggt cgccataccg tagctcggtt tgtctttcgc tggcagtggc agtgcaaacg
    12421 ccgcctggct aatcgggtca cgaccggaac ggcttaaggt tttcggctcg ccaaatttca
    12481 gaccggcagc ctgcatagct tccgcacctt tgccggcttt caaatcaacc agcagtttct
    12541 cagcatccac tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc ttaacttgtt
    12601 cctgaacatc tgccaacggt ttcaccgctt ccggtttgtg ctcgctgacg cgcagcacga
    12661 atgcgcggtc gccgtctacg gtgatgatgt cagagttgat gcccgacgcg ccgttttcac
    12721 ctaccagacc gccgttaaag atagcgtcgg caaccggctt gaagttcaac tcttccggca
    12781 ggttatcttt gctgaaccaa cccgtctgag tggctttaac gccggcagct tgctctgcac
    12841 cggccagaga ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc agcgcatagt
    12901 acgcatcgag ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt acttcgtcta
    12961 acgatttcac tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa ccgaccgaag
    13021 atttgatgac accagacagt tggccttttt ctttcagacc agcatttttc agttcgtcgg
    13081 ggatagtggc atcttctaac caacccatat cgccgccgtt acgggcagag ataatatcgg
    13141 cagatttttc tttggctaat gcagcaaaat caccgccttt attcagctca tcaagtaccg
    13201 ctttcgcttc atcttcagtt ttggtctgga tgatgctgta gcgggtacgc tgcggctggg
    13261 tgaattgatc ctgatgctga tcgtagtagc tctggatatc cgcatcgcta accggttgct
    13321 gcatcgttgc ggcaaccagc ttgatgtaac tcacgcggaa ttgttccggc gtcatgaaat
    13381 tgtttttgtt ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc tgcttcgccg
    13441 ccagcgcgtt aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg accagtgccg
    13501 ccagctcgtc ggtttcacct ttcagcataa aatcggtacc ggcaacgccg ttaatcagct
    13561 gttgggtagt gagctggtta cgcagcgcct gggcgtactg atcggcggtc atccccatct
    13621 ggttgaggat accgttatag cggctgttat caaatttgcc gtcaacctgg aaggctgggg
    13681 tcgcgaaaat cgcctgttta acctgctcat cactgatacc cagtttcagc tcacgagcgt
    13741 actgatccaa cagcgcctcg tcgatcagac gattcagcac ctgttgacgc agggttttca
    13801 tatagccttc gttcgctgcc agctcggagt attgatcgcc cagctgttgc tgcatacgat
    13861 tacgctcgct gttaaaggcg ttctcgaact gcccacggct gatttcctgg tcattcactt
    13921 ttgcggcgta gttattgcct ccgccaatca ggtaaccact cacgccggtc aatatgaacg
    13981 acacgataat gataccgaaa ataatcttga gcacgagact gtttgcagcc gtgcgtaagc
    14041 tgtccatcat ggtgtaacaa cactccgctg tagatgactg tgtacctcac gcaacgtatc
    14101 gatgcgcgca aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc acaagacaca
    14161 gaaagtcacc gaatacaaat aaaaaaggca catcagtaga tgcgcccttg aacttcgtca
    14221 catccccact ggggacaacg cttagtttac cgcgtctttc agtgctttac ctgcacggaa
    14281 gctcggtact ttagcggcag cgatggtgat ctctttaccg gtctgcgggt tgcggccagt
    14341 acgggcagca cgctctttaa cggcaaaagt accaaaacct accagtgcta catcatcccc
    14401 ttctttcaga gattcagtta cggaagcaat aatagcatct aacgcacggc cagccgcagc
    14461 tttagagata tcagcccctg cagcaatctt gtcgatcaat tgagatttat tcactcttct
    14521 cttcctcttt ataatttata tcgcacttga atccttcaag gtacgaacgc gcagcagtta
    14581 tatcaggcca gccatcccct tacaacaccc cttaataagg gcaagcccga tccgctatct
    14641 aacttagcga gacaaaaaaa ggctggcaag cccgaattag cctgccagcc ctgtttttat
    14701 tagtgcattt tgcgcgaggt cactattttg cagtcacaac ctgcatgcca gacggttcat
    14761 tttgcagcgc cagagtcaga acttcctcaa tgcgcttcac aggatgaatg tccagatcgg
    14821 caattacgtt gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga attagcactg
    14881 ttttaatccc gccgcgatgc gctgccagga gtttttcttt caaaccaccg atcggcagta
    14941 cctgaccacg cagagtgatc tcaccggtca ttgccacatc ggcacgaacc gggttaccgg
    15001 tcaggcaaga aaccagcgcg gtgcacatag caataccggc actcggacca tctttcggcg
    15061 tcgcaccttc cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca gggttgatcc
    15121 ccagtttttc cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac tcctgcatca
    15181 cttcgccgag cgaaccggta taggtcagtt tgcctttacc cggaacacat gcggtttcaa
    15241 tggtcagcaa gtcaccgccc acttccgtcc acgccagacc gattacctga ccgacacggt
    15301 tttcgttatc agcgcgacca tagtcgaaac gctgaacacc gaggtagtca tgcaggttat
    15361 cgccgttaat ttcgatatgt tttaatgact tatcgagcag taactgctta accgctttgc
    15421 ggcacagttt ggagatttca cgctccagac cacgcacgcc cgcctcacgg gtgtagtaac
    15481 gaataatgcc gataatggcg ctatcgtcga cggtcagctc accttttttc agtgcattac
    15541 gttcaatctg cttcggcagc aggtgacgtt tggcgatgtt cagtttttca tcttcggtat
    15601 aaccggagag gcgaatcact tccatacgat ccagcagcgg tgccggaatg ttcatggagt
    15661 tcgacgtcgc gacaaacatc acgtcgctga gatcgtaatc cacttccagg tagtggtcgc
    15721 tgaacgctac gttctgctct ggatccagca cttcaagcag tgcagaggcc ggatcgcctc
    15781 gcatgtcaga agacattttg tcgatctcat cgagcaggaa cagcggattt ttcacgccca
    15841 ctttcgccat tttctggatc agtttacccg gcatagaacc gatgtaagta cggcggtgac
    15901 cacggatttc cgcttcatca cgcacgccgc ccagcgccat acggacatat ttacgcccgg
    15961 tggctttggc aatggactga ccaagagagg ttttacctac ccccggcggc cctaccaggc
    16021 agaggatcgg tcccttgatt ttgttgacac ggctttgaac cgcaaggtac tcaaggatgc
    16081 gatctttcac gcgctccaga ccataatggt cggtatcaag gatttcctgc gcctgacgca
    16141 ggtctttttt gaccttgctg cgcgcattcc acggtacctg taccatccag tcgatataac
    16201 cacgcactac ggtcgcttct gccgacatcg gagacatcat tttcagcttc tgcaactctg
    16261 cttccgcttt ctcttttgcc tctttcggca tcttcgccgc gtcgattttg cgcttcaggg
    16321 cttcgttttc gtccggcacg tcgtccattt caccgagttc tttctgaata gctttcattt
    16381 gctcgttcag atagtactca cgctgggatt tctccatctg ctttttaacg cggttgcgaa
    16441 tgcgtttctc aacctgcagc agatcgattt ccgattccat cattgccatc agatattcca
    16501 gacgttcgtt aacgtcggac atctccagaa cggactgttt gtcagccagt ttcagcggca
    16561 tatgtgcagc aatggtatcc gccagacgcg ccggatcgtc gatgctattc agcgacgtca
    16621 gcacttctgg tgggattttt ttgttcagct tgatgtagcc ttcgaactgg ctgattgcag
    16681 tacgcaccag cacttcctgt tcccgctcat caatggtcgg cgactccaga tactccgcct
    16741 tcgcagaaaa gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc tgtaacccct
    16801 cgaccagcac tttgacggtg ccgtcaggca gtttcagcat ctgcaatata gaggccacgg
    16861 tcccgacggt gaaaagatcg tttacacccg gctcatccgt tgaagcttct ttctgcgcga
    16921 ccagcataat ttttttatca tggtccatcg ccgcttccag acaacggata gatttttccc
    16981 gcccgacaaa taaggggatg accatgtgcg gataaaccac cacatcgcgc agcggcaata
    17041 cggggatttc aatgcgttca gaacgctcag gattcataga gctctctctt agtttaattt
    17101 ccgccaggta atcagatgac acgactgtgc ttcacgccat ctattaacat gtacgtcagt
    17161 atatggggat gtttccccca cattcaacgc cgagaataga ggaaaaatta aaggggagat
    17221 aaaatccccc ctttttggtt aactaattgt atgagaatgg ttaattattc accagatgcc
    17281 tgttgcgctt ccggcttacc ataaatcagc aacggtttgc tttgaccatc aattaccgac
    17341 tcgtcgataa ccactttttc gacgtcttcc atggacggca gatcgtacat ggtatcgagc
    17401 agtgcggctt ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc catcgctttc
    17461 ttagcgatag catccagcgc ctcgtcacgg aattccagat ccacgccttc cagattaaac
    17521 agcgcctgat actgcttggt cagggcgttt ttcggctctt tgaggatctg aatcagagct
    17581 tcttcgctca gttcattcaa cgttgctaca accggcagac gaccaatgaa ctcagggata
    17641 agaccaaact tgatcagatc ttccggttca acctgcgcca gcagctcgcc ttcgcttgct
    17701 ttgtcggact tcgcttttac cgtcgcgcca aaaccaatgc cggagccggt ttctacacgg
    17761 tgggaaatca ctttatccag accggcaaac gcaccgccac aaataaacag gatcttagag
    17821 gtatcaacct gcaagaattc ctgctgcgga tgtttacgcc caccttgcgg tggaacagca
    17881 gctaccgtac cttcgatcag tttcaacagt gcctgctgta ctccttcacc ggaaacgtct
    17941 cgggtaatgg acgggttgtc tgacttacga gaaatcttgt cgatttcatc gatgtagaca
    18001 atcccgcgct gcgctttctg tacgtcgtaa tcgcactttt gcaacagctt ctgaatgatg
    18061 ttttcaacgt cttcgcccac ataaccggct tcggtcagcg tggttgcgtc ggccatggtg
    18121 aacgggacgt ccagcaggcg cgccagcgtt tcagccagca gcgttttacc ggaaccggtc
    18181 ggaccgatca gcagaatgtt acttttgccc aactcgacac cattgctggt atcgccgttg
    18241 cgcagacgtt tgtaatggtt gtataccgcg accgccagca cttttttcgc ctgttcctgg
    18301 ccgataacgt aatcgtccag gtggttgcga atttcatgcg gcgtcggtag cgcactgcgt
    18361 tcacgatgcg gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca taaatcaaca
    18421 cattcgtcgc agatatacac ggatggaccg gcaatcagct tgcgcacttc atgctggctt
    18481 ttgccgcaaa aagagcaata cagcaatttg cctgagccat ctttgcgttt atctgtcatg
    18541 ggtcaaaacc tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc cattctcagg
    18601 cgcaagccgc ttacagcgtt gtgccgccct agataagtat agcggcacag ttgcgcctct
    18661 ggcatcaatt acgatgggtc agaatcgaat cgaccagacc gtattccacc gcttcagggg
    18721 cggaaaggaa gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat tgacccgtat
    18781 gaagcgccat aagttcattc atgcgccctt taactttcag aatttcacgg gcatgaattt
    18841 cgatatcggt cgcctggccc tggtagccgc ccaacggttg gtgaatcatc acgcgcgaat
    18901 tcggcaggca aaaacgttta ccttttgccc ctgcggtcaa caagaaagcg cccatcgagg
    18961 ccgcctggcc catacagatg gtgctgacat caggcttgat aaactgcatg gtgtcataga
    19021 tagacatccc ggcagtgatc accccgcctg gggagttaat gtacagatag atatcttttt
    19081 ctgggttttc cgcttccagg aacagcatct gcgccacaat caggttagcc atgtggtctt
    19141 caacctggcc agtcagaaaa atgacgcgtt ccttaagtag acgagaatag atatcaaaag
    19201 agcgctcacc gcgtgaggtc tgttcaatga ccatcggcac cagcgccata tggggtgcaa
    19261 agttatctcg ttcgccgctg tatgacattt ccgtctcctg gataaaattg aaaaaaacct
    19321 gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg
    19381 catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt
    19441 tcgcacggct ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc ggtgacgggc
    19501 ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc atcagctcgt
    19561 tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca acagcctgtt
    19621 cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg tagaactcga
    19681 taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct ttcacgcgct
    19741 cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc cagcagcagg ccaacaacta
    19801 cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt
    19861 tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg tcgatcagcg
    19921 cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga gacttaacgc
    19981 ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca gcgcgcagac
    20041 cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc agttccggca
    20101 gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta cctttcaggt
    20161 tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg ccagctttgt
    20221 ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc gccagtacga
    20281 aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg aagtcgatgg
    20341 ttacgcggtc ttctgcttca acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac
    20401 gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa
    20461 cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac tctacagagt
    20521 aagtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg ccagccggat
    20581 tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca cccagaacgt
    20641 cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact ttgcctttgc
    20701 ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta acagcggtct
    20761 cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga gtggtttcaa
    20821 ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca
    20881 agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac attccctgtt
    20941 accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca tcacttttat
    21001 gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg ggcaattttc
    21061 gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt
    21121 tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat gcgtttttac
    21181 gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg caacccttcc
    21241 cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga gagttcctcc
    21301 gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg
    21361 ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg acgatagctt
    21421 tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc ttctatccgc
    21481 tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca tccctttaaa
    21541 tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg tttcgtcctg
    21601 gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag gcctcgcatc
    21661 agcgtttctt ttaccagggg tgattatttc gtcaacatga tgaatttaca tgccttaccc
    21721 acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac acgcttaaga
    21781 gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg
    21841 cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat tacactgcca
    21901 cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga tcagcgtacc
    21961 gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc
    22021 gctctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg
    22081 gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta tgctgacgtg
    22141 tcccagggca acgtgcgcta caacatcgct accaaagcgg atatcgccat catcgctacc
    22201 gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg
    22261 ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac cgataccatc
    22321 gctgatatgt ctcaggacac cagcattcac gaattcatca agcagaacgc gcgttaattt
    22381 ctgccctctg gcccggtgca agccgggcct gtagacgccc atgtccagtc aatatttacg
    22441 tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt ccgggctacc
    22501 gctcgccctg acatccggca ccttacaggc ctggatgacg gtcgaaaata tcgatctcaa
    22561 aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc tctggtcacc
    22621 gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc tgctcgccac
    22681 gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaaccag gcacccaact
    22741 ccgctggatg gcggcgctgg cggtggtgat cgctttttgc tctgcctccc aggatattgt
    22801 cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat
    22861 cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcctagccc tgtggctggc
    22921 agataaatgg ttgggctggc agggcatgta ctggttgatg gcggcgctgt tgatcccctg
    22981 tattatcgcg acgttgcttg caccagaacc aaccgacacc attcctgtgc ccaaaacgct
    23041 ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg cctggcttat
    23101 tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc tgacaaccac
    23161 gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtaa acaaaacgct
    23221 tggcttactg gcgaccattg ttggcgcatt gtacggtggg attttgatgc agcgcctgtc
    23281 gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg ctggttactg
    23341 gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct ttttcgaaaa
    23401 cctctgtggc gggatgggca catcagcctt tgtcgcgctg ttaatgacgc tgtgtaataa
    23461 gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag ggcgagttta
    23521 tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat tctatctatt
    23581 ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc agacgcttga
    23641 atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccggcag gttatgcctt
    23701 tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac ttctgttgac
    23761 gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg aagtcggggt
    23821 tttagtcgcc ctttctggcg tcatgcttgg tggtttgctg gattatctgg cgctacgaaa
    23881 aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg ccgtaattat
    23941 tacggctaaa taaacatcag catcgcttat taatatttgt tcttttgtgc ggcttagcgt
    24001 ttggaaatta ttgacgccat ttataaattc tatttttctt acacgattca gctaatgagt
    24061 ctttattttc tcaccaccca gttgtcactt taatgataat tatttgttaa ataattgttt
    24121 tatttcgcat tggttatacc aattgcccgc caagactcct tctgcactcc ccttttgtta
//
LOCUS       DAJVRL010000060        23956 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVRL010000060 DAJVRL010000000
VERSION     DAJVRL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.060"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(1..374)
                     /locus_tag="OQN02_002399"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="OQN02_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="OQN02_002400"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="OQN02_002400"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1643160.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="OQN02_002401"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="OQN02_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1643161.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="OQN02_002402"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="OQN02_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1643162.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="OQN02_002403"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="OQN02_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1643163.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="OQN02_002404"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="OQN02_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1643164.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="OQN02_002405"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="OQN02_002405"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1643165.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="OQN02_002406"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="OQN02_002406"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1643166.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="OQN02_002407"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="OQN02_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1643167.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="OQN02_002408"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="OQN02_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1643168.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="OQN02_002409"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="OQN02_002409"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1643169.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="OQN02_002410"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="OQN02_002410"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1643170.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="OQN02_002411"
     CDS             10736..11161
                     /locus_tag="OQN02_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1643171.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="OQN02_002412"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="OQN02_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS1643172.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSDDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="OQN02_002413"
     CDS             12185..13621
                     /locus_tag="OQN02_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1643173.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="OQN02_002414"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="OQN02_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1643174.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="OQN02_002415"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="OQN02_002415"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1643175.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="OQN02_002416"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="OQN02_002416"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1643176.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="OQN02_002417"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="OQN02_002417"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1643177.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="OQN02_002418"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="OQN02_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCS1643178.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="OQN02_002419"
     CDS             complement(20454..20747)
                     /locus_tag="OQN02_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1643179.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="OQN02_002420"
     CDS             complement(20744..22033)
                     /locus_tag="OQN02_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1643180.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="OQN02_002421"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="OQN02_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="OQN02_002422"
     CDS             complement(23047..23811)
                     /locus_tag="OQN02_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCS1643181.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5922 a         5809 c         6308 g         5917 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgagtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgacga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaa
//
LOCUS       DAJVRL010000061        23840 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVRL010000061 DAJVRL010000000
VERSION     DAJVRL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.061"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(2..94)
                     /locus_tag="OQN02_002423"
     tRNA            complement(2..94)
                     /locus_tag="OQN02_002423"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OQN02_002424"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OQN02_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1643182.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OQN02_002425"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OQN02_002425"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1643183.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OQN02_002426"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OQN02_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1643184.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OQN02_002427"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OQN02_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1643185.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OQN02_002428"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OQN02_002428"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1643186.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OQN02_002429"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OQN02_002429"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1643187.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OQN02_002430"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OQN02_002430"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OQN02_002431"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OQN02_002431"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1643188.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OQN02_002432"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OQN02_002432"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1643189.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OQN02_002433"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OQN02_002433"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCS1643190.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OQN02_002434"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OQN02_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OQN02_002435"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OQN02_002435"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1643191.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OQN02_002436"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OQN02_002436"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1643192.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OQN02_002437"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OQN02_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1643193.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OQN02_002438"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OQN02_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1643194.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OQN02_002439"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OQN02_002439"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS1643195.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OQN02_002440"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OQN02_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1643196.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OQN02_002441"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OQN02_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1643197.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OQN02_002442"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OQN02_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1643198.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OQN02_002443"
     CDS             complement(20900..21019)
                     /locus_tag="OQN02_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCS1643199.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OQN02_002444"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OQN02_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1643200.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OQN02_002445"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OQN02_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1643201.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPLDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..23822)
                     /locus_tag="OQN02_002446"
     CDS             complement(23682..23822)
                     /locus_tag="OQN02_002446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643202.1"
                     /translation="MLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSR
                     GA"
BASE COUNT         5610 a         6697 c         6094 g         5439 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctagcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccagttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtacattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat cccgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtctaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt
//
LOCUS       DAJVRL010000062        22623 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVRL010000062 DAJVRL010000000
VERSION     DAJVRL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.062"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..434)
                     /gene="pgrR"
                     /locus_tag="OQN02_002447"
     CDS             complement(<1..434)
                     /gene="pgrR"
                     /locus_tag="OQN02_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1643203.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
     gene            572..1492
                     /locus_tag="OQN02_002448"
     CDS             572..1492
                     /locus_tag="OQN02_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1643204.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1492..2428
                     /locus_tag="OQN02_002449"
                     /pseudo
     CDS             1492..2428
                     /locus_tag="OQN02_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            2446..3084
                     /locus_tag="OQN02_002450"
     CDS             2446..3084
                     /locus_tag="OQN02_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1643205.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3375..4103
                     /gene="mpaA"
                     /locus_tag="OQN02_002451"
     CDS             3375..4103
                     /gene="mpaA"
                     /locus_tag="OQN02_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1643206.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4078..5037)
                     /gene="ycjG"
                     /locus_tag="OQN02_002452"
     CDS             complement(4078..5037)
                     /gene="ycjG"
                     /locus_tag="OQN02_002452"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS1643207.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5156..5662
                     /gene="tpx"
                     /locus_tag="OQN02_002453"
     CDS             5156..5662
                     /gene="tpx"
                     /locus_tag="OQN02_002453"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1643208.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5706..7247)
                     /gene="tyrR"
                     /locus_tag="OQN02_002454"
     CDS             complement(5706..7247)
                     /gene="tyrR"
                     /locus_tag="OQN02_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1643209.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7395..8456)
                     /locus_tag="OQN02_002455"
     CDS             complement(7395..8456)
                     /locus_tag="OQN02_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1643210.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8453..9850)
                     /locus_tag="OQN02_002456"
     CDS             complement(8453..9850)
                     /locus_tag="OQN02_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1643211.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10006..11004
                     /locus_tag="OQN02_002457"
     CDS             10006..11004
                     /locus_tag="OQN02_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1643212.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11115..11987)
                     /gene="ompG"
                     /locus_tag="OQN02_002458"
                     /pseudo
     CDS             complement(11115..11987)
                     /gene="ompG"
                     /locus_tag="OQN02_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(12060..13141)
                     /locus_tag="OQN02_002459"
                     /pseudo
     CDS             complement(12060..13141)
                     /locus_tag="OQN02_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13155..13814)
                     /gene="pgmB"
                     /locus_tag="OQN02_002460"
     CDS             complement(13155..13814)
                     /gene="pgmB"
                     /locus_tag="OQN02_002460"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS1643213.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(13811..16078)
                     /gene="ycjT"
                     /locus_tag="OQN02_002461"
     CDS             complement(13811..16078)
                     /gene="ycjT"
                     /locus_tag="OQN02_002461"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS1643214.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16075..16698)
                     /locus_tag="OQN02_002462"
     CDS             complement(16075..16698)
                     /locus_tag="OQN02_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1643215.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(16695..17117)
                     /locus_tag="OQN02_002463"
     CDS             complement(16695..17117)
                     /locus_tag="OQN02_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1643216.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(17139..17914)
                     /locus_tag="OQN02_002464"
                     /pseudo
     CDS             complement(17139..17914)
                     /locus_tag="OQN02_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(17932..18984)
                     /locus_tag="OQN02_002465"
     CDS             complement(17932..18984)
                     /locus_tag="OQN02_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCS1643217.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19015..19857)
                     /locus_tag="OQN02_002466"
     CDS             complement(19015..19857)
                     /locus_tag="OQN02_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCS1643218.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19844..20725)
                     /locus_tag="OQN02_002467"
     CDS             complement(19844..20725)
                     /locus_tag="OQN02_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS1643219.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20746..22029)
                     /locus_tag="OQN02_002468"
                     /pseudo
     CDS             complement(20746..22029)
                     /locus_tag="OQN02_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(22043..>22623)
                     /gene="ycjM"
                     /locus_tag="OQN02_002469"
     CDS             complement(22043..>22623)
                     /gene="ycjM"
                     /locus_tag="OQN02_002469"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCS1643220.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5574 a         6085 c         5513 g         5451 t
ORIGIN      
        1 acgcctgcat caaaacgttc agaaacgaca tcggtcaaac cataatcaat ggtaagctga
       61 atatcaattt caggatgcga tttcaggaat gtgcgcatgg ctggtaacaa tatcgttttt
      121 gctgcatgtt ctacagtagt aatacgtatt gtcccggatg ggcggttctg cagatcgctc
      181 agggatgtca gggcagaatc tatgtcatgc aatatagggc caagaacaga caaaagatgc
      241 tcgcccgctt cagtgggtac aacgctgcgc gtggttcgcg tcagaagccg caatcccaat
      301 cgttcttcta tacggcgcac tatctggctt aaagctgact gcaccatgct caggcgggct
      361 gctgcgcgag tgaagctacg ctcctctgca acgacgacaa acgccatcag atcagcgatt
      421 tcttcacgct tcattcagta tctccataca atctattaat catttaaatg ataggtctaa
      481 tcatgattta gggtctaatc aacttaatga tcaatatcta tagtgatatc accaaccgtc
      541 aaaacgaacc gacaactcca tgaggaactg aatgatgaat aataaagtca gcttcactaa
      601 cagcaataat ccaaccatct ctttgtctgc agtgatctat ttccctccaa aatttgatga
      661 aacccgtcag tatccggcaa ttgtggtatc tcacccaggt gggggcgtta aagagcaaac
      721 ggccggaacc tatgccgaaa agctggcgga aaagggattt gtcactattg cttatgacgc
      781 atcttatcaa ggtgaaagtg gcggcgagcc gcgtcagtta gaaaacccat acattcgtac
      841 tgaagacgtc agtgccgtga ttgattacct gacaacgctt tcttatgtcg acaatacccg
      901 aattggtgcg atgggaattt gtgccggtgc aggatataca gccaatgctg caattcagga
      961 tcgccgcatc aaggccatcg gtaccgttag tgcggtcaat attggttcga tgttccgcaa
     1021 tggttgggaa aacaatgtga aatctattga tgctctgcca cacattgaag ctggctcaaa
     1081 cgcccgaacc agcgatatta gcagcgaaga atacgccatt atgccactgg ctccaatgaa
     1141 ggaggccgat gcgccgaacg aagaactgcg tcaggcctgg gagtattacc acacacctcg
     1201 tgcgcagtat ccaacagcac cgggttacgc tactttgcgc agccttaacc agattattac
     1261 ctatgatgct taccatatgg cggaagtgta cctgactcag ccgatgcaga ttgtggcggg
     1321 cagccaggca gggagcaaat ggatgagtga cgacctgtac gatcgggcat caagccagga
     1381 taaacgctat cacattgttg aaggcgcaaa ccatatggat ctttatgacg gcaaagccta
     1441 tgtggctgaa gcaatttcag tattagctcc gttctttgag gaaactctgt aatgaaacat
     1501 attcaaatcc gcaatagtga tatggcctgg catattgctg ccaatattca gtttccacca
     1561 aattttgatg agtcaaaaca gtatccggcg attatcagcg tccatccttt tggcagttgt
     1621 aaggagcaga cgtctggcaa tatctatggc aaggcattgg ctgaaaaagg ctatgtcgtt
     1681 ctcgcttatg acgccagttt ccagggagaa tctggtgggt agcctcgttg gatagaagat
     1741 ccgacacagc gcgtagagga tattagccgt gttatcgatt atgcagtaac tctgccatat
     1801 gttgatgctg aacgcattgg cgtgctgggt gtgtgggggg ggggggggtg gctatgccat
     1861 taatgccgcg ctgacagaaa aacgaattaa agctgtagtg agtattacgg gtgttaatat
     1921 cgggcgtctt tttcgggaag gtttcagcaa ttatgatccg attggcgtat tgaatgcaat
     1981 ggcgtcacaa cgagcaaaag aggctcgtgg tggagaatta cagatcaacg aattacttcc
     2041 cgccagcctg gatgcagcaa aagcccatgg actgaccgaa cgcgatgttt atgaagcaac
     2101 tgactactat aaaacaccac gcgggcaaca accgggaggt gcgacaaaaa tgttgttctc
     2161 acatgcacag aaaacgctgg cctgggatgc cttcgcattt actgaagtgt tactgaccca
     2221 gccggtgatg gtagtagttg gtgaaaaagt cggtgccttc ggtgcttatc gtgatggact
     2281 ggaagtctat ggccgggcga tggtttcgca ggatcgtcag ttagtttcgt tacccgattt
     2341 ctcacattat gaactatatg acaaaccgga agcagttcag gaggcgctgg caaaagttat
     2401 tccttttttc aacacccatc tgggataagt ttcatgagaa atattatgaa aaatgtactg
     2461 attcttggtg ctggcggtca gatagcccgc catgtgatta atcaactggc agataaacaa
     2521 actattaaac aaactttatt tgcccgtcag ccagcaaaaa tacacaaacc ctatccaact
     2581 aatagccaga ttattatggg ggatgtactg aatcatgcag cactgaagca ggccatgcag
     2641 ggacaggata ttgtgtatgc caaccttacg ggggaagatt tagatattca ggcaaattca
     2701 gtgatcgctg cgatgaaagc ctgtgatgtt aaacgtctga tctttgttct ttccctcggt
     2761 atttatgatg aagttccagg aaaatttgtc gaatggaata atgcagttat tggtgaaccc
     2821 ctaaaaccat tccgcagggc tgcggatgca attgaggctt ctggactgga atatacaatt
     2881 cttcgccctg cctggctgac tgatgaagat attattgatt atgagcttac ctctcgcaat
     2941 gaaccattca aaggtacgat cgtatcacgt aagagtgtcg cagcattaat tacagatatc
     3001 atcgataaac ctgagaaaca cattggtgag aatataggca tcaatcaacc cgggactgat
     3061 ggcgataagc cattcttcat gtaaatcaat tatatagata ccatcgtact gtactatgtg
     3121 cagtacgatt tattaaactt attaaggtga actgatcaat cagatgcgat taacatgtgt
     3181 agaaccagta agaataaaat acgccatgta tataacatta cgacaattct aatatctgca
     3241 catcaatgaa aatgcaaaca acttccggca aaaaagtgat acaagatcga tgacatagcg
     3301 ttgaagaaaa ggatttgata atccgcaaaa tttgcacaac actgcctgta atacttcctg
     3361 aaccagagac gatcatgacc gtaacccgct cacgcgccga acgcggcgca tttccgcccg
     3421 gaacagaaca ttacggacgt tcattattgg gcgcgccgtt aatctggttt cctgcccctg
     3481 cagccagccg cgaaagtggt ttgattctgg ctggcactca cggtgatgaa aactcttcgg
     3541 tcgtcacgct ctcctgtgct ctgcggactt tgacaccttc tttgcgccgc catcatgtgg
     3601 tgctgtgtgt gaatcctgac ggctgccagc tggggttacg ggccaatgct aatggtgtgg
     3661 atttaaacag aaactttccg gcggcgaact ggaaggaagg tgaaacggtt tatcgctgga
     3721 acagcgccgc tgaagaacgt gatgtcgtat tactgacagg tgacaaacct ggctccgaac
     3781 ctgaaaccca ggcgctgtgc cagcttatac accgcattca acctgcctgg gtggtctcat
     3841 tccacgatcc actggcttgt attgaagatc ccagacacag cgaattaggc gaatggctgg
     3901 cccagtcgtt tgaattgcct ctggtaacca gcgtcggtta tgaaacgcct ggctctttcg
     3961 gcagttggtg tgcagatctc aacttgcatt gcatcaccgc agagtttccg cccatctcct
     4021 ccgacgaagc cagcgaaaaa tatctctttg cgatggcaaa cttgctgcgc tggcatccta
     4081 aagatgcaat tcgcccgtcg tgaactgaag cgccggttcc acatctaccg ccagccaggt
     4141 cggtccgtca agatcggcga aactgacctg cggcaccagc ggtaaagcgg cgctaatggc
     4201 acgagaggta cacaacatgc agcccagcat cagactgaaa ccttgtgcac gcgcttcagt
     4261 cgccagcgct tccgtcagac caccggtttt atcgagctta atgttaacca tctcatagcg
     4321 ccctttcagc gccttcagat tgctacgagt atgacaactt tcatcagcac aaatcggcaa
     4381 cggatgaata aaattctcca gcgccgcatc gtcctgcgca ggaagcggtt gttcaagcat
     4441 cgcaacgcct aaatccgcca atagctggca acgcgccgcc aacccttctg cacgccagga
     4501 ttcatttgca tcaacgatca gcgtggcatc gggcacagcg gtgcgaattg ccaccatccg
     4561 ctcactgata agatggttat ccagcttcac tttcagtaat ttcgcgcctg cctgccagag
     4621 tgttgatgca ctattggcca tctgatcagg cgtaccgatg acaaccgtct gtgcagtgat
     4681 aactgtctcg ggaagcgtta tgccgatcaa atcagccagc gattgctgct gttttcgcgc
     4741 cgcgagatcc cacaatgcac aatccagcgc attacgtgct gcgccggcag ggagaatttt
     4801 ttgcaactcc tcccgtgtca gccctttctc taattgcggc acgacgctca taatttgcgc
     4861 cattaccgag gcatcacttt ctccataacg cggatacggc gtgcattcgc cggtgccttt
     4921 aataccctct tcttccagtt caaccaccac cacgcgcgct tcactgcgac ttccccgggc
     4981 aatcacaaac ggggtatgta agggccaggc ttcctcaaat accttaacga ttctcatctt
     5041 tactccttgt tgtcgcggtg gtctgcggat taagcgcaaa tagcgtttgc tgtgttattg
     5101 acagttagca taaactaggt gtgacgttaa ctatatgtaa acaggaaaga taatcatgtc
     5161 acaaaccgtt catttccagg gcaacccggt tacagtcgcc aattccatcc cgcaggcggg
     5221 tagcaaagcg cagactttta ctctcgtggc aaaagatctg tctgacgtca ccctcggtca
     5281 gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt attgataccg gtgtttgcgc
     5341 cgcatcagta cgtaagttta accaactggc aactgagatc gacaacaccg ttgtgctgtg
     5401 tatctctgcc gatctgccgt tcgcccagtc tcgtttctgc ggcgcagaag gtctgaacaa
     5461 cgttatcacc ctctccactt tccgtaacgc tgaatttctg caagcctacg gtgtggcaat
     5521 tgctgatggc ccactgaaag gtctggcagc gcgtgccgtt gtggttattg acgaaaatga
     5581 caatgtgatt ttcagccagc tggtggatga aatcactacc gagccggatt acgaagcagc
     5641 tctggctgta ctgaaagcat aagttaatat gcctgatggt gttgcaccat caggcatatt
     5701 cgcgcttact cttcgttctt cttctgactc agaccatatt cccgcaactt attggcaatc
     5761 gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg tgctgggata attgcgataa
     5821 agctgggtta ataccgagcg ttcaaaacgg ctggtgattt cgtccagcga accttccatc
     5881 gcatcttcgc ccacggctac cgttgcggcg tcataatccg gcaacaaaat atcctgtgga
     5941 cgcagctcat aaccgtccag ttgtgtcagt gcgcgataga tagcgttctt taactgccgc
     6001 acatttcccg gccacgcata acgtgtaagt acagtattca ggtcagcggc cagtttcgga
     6061 cgcggcacgc cctgctcgtc ggcaaagcgg gcgacgaaca gctcagttaa cggcataatg
     6121 tcctgcggac agtcacgtag cggcggcaga ttgagcgtca acacgttcag acgataatag
     6181 agatcttcac ggaacacgcc tttttgcacc agttcgacca gattcttctg cgtagcgcaa
     6241 atcacccgca catcgacatg cacctcatgg tcttcgccaa cccgacggaa agtgccatca
     6301 ttaagaaaac gcagtaattt cgcctgcatc cgtggtgaca tttcccctat ttcatccaac
     6361 agcaccgaac caccgttcgc ctgctcaaag aaccctttct tcccttccgg agcatgacca
     6421 aacagttcac tctcgaccgc atcttctggt atagacgcgc aattcagcgc caggtaaggt
     6481 ttgcccgctc tggggcttgc ctgatggcag gcgtaggcaa aaagatcttt acctgtacct
     6541 gtgtcacccg taatcagcag cggcgcgctt agcatcgcca gtttctgcgc ctgttcgaca
     6601 acgtgcttca ttttcgggct gacggcgaca atttggctga aggcgctgac gtcctgggcg
     6661 gcgacatttt gcaactggcg gcccatacga atcgttgatc gcaacatcac caccgcaccg
     6721 gtcaggacgt gttgatcatt ttcatcctga agataaacag gcgtaatctc catcaggaaa
     6781 ttctgcccat taataacgac atgctcgtta tgcgaatctt gcggttcgct ttccagccaa
     6841 cgtaaaaaat taaagccgtt aatcaattgt gcggcggtat ggttgcgcag gcgatccaat
     6901 ttttgcccaa aaagctgaca gctcgccggg ttcgccatat ccactttgct tttcatatcg
     6961 acagagagca caggttcagg caacgcctcc agtaacgcgc tcaacgccag atgctcacgt
     7021 tcggaaggca tccacgggac agtacgcaca tcggtaacac ctgcaatacg gcgtatttcg
     7081 gccatcagac tgctgaaact ctcaaactcc agttcagcaa aattgaggta gattcgccca
     7141 atgggatcaa tctcaatacc gcgtaaatca atgcctctta gcacgagtag atcgagtaat
     7201 tcgcgggtca gaccgagtcg gtcttcacaa aagacttcca gacgcatggg aaccttcacc
     7261 tgaaaaaaga acaattaata tgatgatatg acactatttg gatagcagga aggtttctgt
     7321 caacaatcat tgacacaaag acggaaaaac gggcacttcg gcgtaaagat atccggtcag
     7381 cacccgatat tgcattattt ttcgctgggc gttttgcctt tttgcagagt ttctttcacc
     7441 tgaccgataa gctgacgacg gaaatcccca aggcgaggtt tgtcatcgtc aatccacggc
     7501 agcgggcggc aaagctccat agctttaatc ccgagtcgta ccgtcagaag tccagcacca
     7561 attccctgag ctgcgcgggt agacaaacga gcagcgagat cttgcgacat ccagtccatc
     7621 cccacttcgc gcaccagttc gctggctccg gcaaaagcga tattcagcaa taccagctta
     7681 aacagacgca aacggctgta ataccccagt tcaatgccat acagcgtggc aatgcgatta
     7741 attaaacgca gattgcgcca ggcgataaac gccatatcga ccaacgccag cgggctgacc
     7801 gcaatcatca acgttgattc cgccgccgaa tggctgattt cgcgccgcgc ctgggcatct
     7861 aaaactggct ggaccaaatg cgcatacagg ctgaccactt cacgatcgtt ctgcgtttca
     7921 tggattgagg cataccagcg ttgcagcgcc ggatgcgact gatcaatacc cgcctgctgc
     7981 gccagttttt cgcaaaatgc gcgacctttg cccgtgccgt ggctatgcaa taaatcacgc
     8041 gcttcgtcgc gttcatgggc gcgctgtcgc aagcgccata agcgccgcca ctctgttacc
     8101 acagaaccta cgccagcgcc gataatcaat gccccagcgg cacatccacc cagcgctacc
     8161 cagtcctggg tttgccaggc attcattgtc cactgtatac cctgcccgac aacgcttgcg
     8221 ccaaacagag ccagcccgcc catcaccatt ttgcgccaca ggctgcgttt cggacgtaac
     8281 gctgcgtcca ttaccgcttc gacttgcccc tcttcttcct gcgcttcgtc gagcgtggcc
     8341 ggggcaaaat tttgcgcctg attttcgtca aaggtctgct gcgccctgaa ttttggattc
     8401 tgttcgacct ccagcggacc gtcgaaatca atacgtggtt ttaacggttc ggtcatcgca
     8461 atttatctcc tattaaaaat tccagcgcgg catcaagacg aatatgcggc agcggtttgt
     8521 cgacatccat cacctgcggg cgaaacgctt caaactggaa cccttgcttg tcccagaacg
     8581 cctgaccggg caaacgcgca ggaacttcac cggggtaaac ggtgagaggc gcaccatcgc
     8641 tgagccggtt accgcgcaat gccggaattt tctcgccgtt gacgtcaata atgccgctgg
     8701 tggtcgcctg caccgaggcc agccccaggc aatccatact aatcctttca aacgccgcat
     8761 tttgccaggc atcctgaatc agttgttgca gtaatgaaac catattggcg tgctgatcga
     8821 ccgttacatg gtccgctttc atcgcggcaa acagcagttt gtcgataacc ggcgagaata
     8881 agcggcgaaa cagcgtgcgc tgcccgtagt ggaaactttg catcagctgc gtcagtgcca
     8941 gacgcatatc attaaatgcc tgtggcccac tgttgagagg ttgcaggcaa tccaccagca
     9001 caatctggcg gtcaaagcgc agaaaatgat tcttatagaa ccccttcacc actttctcgc
     9061 agtaataatt aaaccgctcg cgcagcattc cggcattggt gtgcttatcg gcctgcgcca
     9121 gtttggactc gtcccaggca tcgacatccg gccacgggaa gaattgcagc gcgggcgcac
     9181 ctgccatatc tcccggcaag acaaagcgcc caggctgaat aaagtgcagc ccctgctgtt
     9241 tacagtggtg gagataatcg gtccacacgg cggcaatgtc tgccagccgg ttttcgtcgg
     9301 caggcgctag cgggtcaagt ccttcgcaca tcattcgcca tttcgccgac cattcgccgc
     9361 gctgaccatt gagtaagccc gtcatctggc gcgaccagct taagtaatcc tgcgccagca
     9421 tcggcaggtc gagcaaccat tcgccgggat aatccacaat ttccagatac agcgtggagg
     9481 tatccttaaa gtggcgcagc agcgaatcgt tcgatttaaa acgtaacgcc aggcgaattt
     9541 cactgacgcc gcgcgtaggc gtcggccagg cgggaggatc accatataac tgcgccagtc
     9601 cttcatcata ggtgaagcgc ggaatgccaa agtcacgctg gggaatgcgt tttacgccca
     9661 gcaagcgctc ttcacgcacc gcgcttaaca gcggcaaacg tgctccggca tgaatattga
     9721 gcaactgatt aaccatcgcg gtgataaacg ctgttttgcc gctgcggcta agtccggtta
     9781 cagcgaggcg cagatgtctg tcgacacccc gattcaccag cgcattgagt tcatttttaa
     9841 gtcgcttcat tgctgtcctg tgctgctctg aagaccagaa aaaaaatcat tcgtaaggat
     9901 atgaatgtgg gggccaggcc attgattatc aactgcccgt gttgtcatcg acgggcgaac
     9961 tccataagat gtgcagcatt cacgttcgga cggagttcct gacctatgtc ccctactatt
    10021 tatgatattg ccagggttgc aggcgtatca aaatccaccg tatcacgcgt gctgaataag
    10081 caaactaata tctccccgga agcgcgcgaa aaagtgttac gggccattga agaattacag
    10141 tatcagccaa acaagctggc ccgcgcgctg acctcttcgg gttttgatgc cattatggtg
    10201 atttctaccc gttcgaccaa aactacggcg ggtaatccgt ttttctctga agttttgcat
    10261 gccatcaccg ccaaagcgga agaagaaggt ttcgacgtga tattacaaac gtcgcacaat
    10321 ccggcagaag atctgcaaaa atgcgaaagc aaaattaaac agaaaatgat taaaggcatt
    10381 attatgctca gttcgccagc ggataagtca ttttttgccc aactcgataa atacgatatt
    10441 ccggtggtgg taattggcaa agttgaaggt caatatgccc atgtttattc tgtcgatacc
    10501 gataattttg gtgacagcat tgcgttgacc gatgcattaa ttgaaagtgg gcatcaaaat
    10561 attgcctgcc tgcatgctcc gcttgatgtc catgtttcgg ttgatcgcgt taatggctat
    10621 aagcaaagtc tgtccgcgca taatattgca gtgcgtgata aatggattgt tgacggcggt
    10681 tatacccatg aaacagcact gcaagcagca cggcaattat taagtcagtc accgttgcct
    10741 gaggcagtgt ttgccactga cagcctgaaa ttaatgagca tttatcgtgc ggcggcagag
    10801 aaaaatattg ctattccgca gcagttagcg gtggtgggtt atagtaatga aacgctgtcg
    10861 tttattttaa cgcctgcacc aggcggcatc gatgttccga cgcaggagtt aggtcaacaa
    10921 agctgcgagt tattattccg cttaatttcg ggaaaaccgt caccacagaa tattaccgtt
    10981 gccacgcata tgacgctgaa ataaatacca gctaccttgc ctgaatgccg gatgcgatgc
    11041 tggcgcatct tatccggcct acatttatag atcggataaa gtactcgcgc cgcatccggc
    11101 aatttagccc attatcagaa cgagtaattt acgccgacac ctgcataatg gaatttatcg
    11161 ctgtcgcctt cgtcgtgatc ctgccactca aacgcgtatt ccagcgaaac ggaaagtccg
    11221 ttctggaaat cataaccgta aaataaaccc acacggttaa aatcatggcc ttcacgttca
    11281 atatcatcct gccagtccca gttactctag cgatccagcc caatgcgcgt atacggcgtc
    11341 accgagtggt tgccgagcgt cagcggcaaa taggcgcgaa tttcttgtgt ggaaaactca
    11401 ccgttattgc ggctgtcgtc catattgaag ccgcgctcga gataatagtt cactcgcaaa
    11461 gcaaccgttt cgttgaaggt atattgcaga cctgtttccg tttcgacacg ggtatcagcg
    11521 taaccggtcg tgttcaaatc gttggcaaat ttatacatcg acaaccaacc gttgaaacgt
    11581 aaatcatcag tcagtttcac atcccagtct ggcgcgattt tccagcgctg catattcgcc
    11641 gtgtctttgc ccggttcatc aacgtagtga taaccataat tacggaaacc gccggtcagg
    11701 ccgaaactga aatcatcgct ttcgaggaac tgataatgca cctccagctc cgggcgatca
    11761 aacctcgttc cacgtttacc cgcgctataa tctaccggcc cttcctgata ataggccaga
    11821 gcaattctcc acggcccgtt ggcggcatta aaatagactg aaggctccgc cagcccatcc
    11881 atatcttcgc cataaccctc gacgttttct atttcgtaca tcgcaccgat attaaagtgc
    11941 cagtcgctct tttcctcggc ctgtgcgcag gccattcccg cacacatcac cagtgcggta
    12001 cagggtaata actttttttg ttccttgtat tatcctgggg gaatgccttc cccctgtatt
    12061 tagcgaattg ctatttccgt ttctgcatca aagaaatgac atttcgtcat atcaaaatga
    12121 atagtgatat tttctcctgc atgataatca tttaacgcac cagcacggac cactaactcg
    12181 tgcccccaac cgtggtgtag agcataaatt ccgcaccggt taattctgcc acgctaattt
    12241 tggcggaaat gttattttcc tcttgcgcgt ccggatgaat atcttccggg cgtattccca
    12301 tcacgatggg cttatgcaaa ctttcctgtg ttttcagaac cgctaatttc tcttcgagaa
    12361 tggttaattt aagcgtttcc gtaacgaatt tatcgccatc gatcgtgccg cgaataaaat
    12421 tcatcgctgg tgatccaata aatccggcaa caaacatatt cgcgggttgg ttataaacgg
    12481 ttttcggcgc acctacttgc tgaacaatcc cgtctttcat aatcacaatc cgcgccgcca
    12541 tggtcatcgc ttcggtctga tcgtgggtca cgtagatcat ggtggtgttc agtttctgat
    12601 gcagcttgct gatctctgca cgcatttgta cgcgcagctt ggcatcaagg ttagagagcg
    12661 gttcatccat taaaaacacg cccgcttcgc gcacaatcgc ccgcccgagc gccacacgct
    12721 ggcgttgccc gccggaaagc gcgcccggct tgcgtttcag gtactcacgc aggccgagaa
    12781 tttgcgccgc ccagttcacc cgctcatcaa tcacctcttt ggcgattttt tgcatcttca
    12841 ggccaaacgc catgttgtcg taaaccgtca tatgcggata caacgcgtag ttctggaaca
    12901 ccatcgcgat attgcgtgct ttggctggaa cgtcattcat tcgtttgccg tcgatcaaca
    12961 gatcgccgcc gctgatctcc tcaagcccgg caatcatgcg cagggtggtc gatttaccgc
    13021 agcccgacgg gccgacaaat acgatgaact ctttatcggc aatttccagg ttgaagtcct
    13081 tcaccacatg cacctggtta tcgtagattt tttgaatatg ttgtaacgaa agctgagcca
    13141 tgttgattcc tttgctatac gttttgccag aaggccgata accgcgacca ggtcagggag
    13201 tccgttgagg gcaacagtaa ttgcgccccg gttaagcccg caccgatccc caccgagcgc
    13261 ataccgctgg cgttaatggc gtcaatgccc gcctgcgcat cttcaatgcc gatacatgcc
    13321 tgcggcggca cgcccagccc ggcacaggca gcgagaaaga tttccggatc cggtttcgag
    13381 tttttaagtt gggaagcatc cgcgcagaag gtgaaaaact cgcgcagctc cagcgccgct
    13441 aaaatcgtcg gcgcattcag ggagacggaa gccagcccta ccggaatctg ctgtgcacgg
    13501 agctctgcca gcaaattgcg aataccgggc agcacagcat taaccgtcag ctcgcgcagt
    13561 gagtggacgt aaagcagatt tttgcgatac gccagttgcg cccgctcctg cgggttaaag
    13621 tcgccctctt tgcccccgtg ttgcagaatg cgccgcagag actcatcgcg gctgatccct
    13681 tttagagatt cgttaaactg cgcatcaata ctgatgccaa tttcagcggc aatctgctgc
    13741 cacgcctgga aatgcagatg cgcggtatcg gtgattacac catccagatc gaaaattacc
    13801 ccttgcagtt tcattcatcc tcctgatgcg tggtagcggt cccattgaag ggcaaaataa
    13861 aatcacccaa acagaaaaca gattcttcag ccacggatat aagctgaccg ttcaaacgca
    13921 gtgaaacggg cgcagaagtt cgaatcgcaa tacgctgcgc gtcgagagtg acctgtaatt
    13981 cgcagccctg ccagaacaga gggaaagaca actgttgcca ctgctcaagt aacgccggat
    14041 ttagatgcaa ttcaccgtca cgcacgctca ccccggcaaa accctgaatc gcccccagcc
    14101 agatagcgcc agttgcggca gcatggatac catcatcaca actatgcgga tcagcaccaa
    14161 gatcgatttc agtcccctcg cgccagaact gataactttg ggtcagcagg ccacagcgtg
    14221 cagcaacaat gccgtggatt gctttactta atgacgagtc gtgaatagtg cgcggttcat
    14281 aaaattgcag attggcaaga cacgatgccg atgagaactg ctctggcagc atgtaattga
    14341 gcatcaccac atcagcttgt ttgaggatct gcatctcgtt cacttctgcg cgtgaataat
    14401 ccagcagaat ggtttgcttc cccgccgccg ctttgtattt cgccagatta atcgccggct
    14461 tagtcataaa cgaatcatcc tgcggcaaaa caccgtcggg ctgaatttct ggcagccgca
    14521 gttctttaag gaacatttcg gcacgatgaa taaacgcgtc gtcactacag ccaaactggc
    14581 gggcaatact cagcgcctgc tggacgttgt aatacaccat atagctggtg aaggcgttat
    14641 tattgacatg ttcggtatat tcgtctggcc caataacatc atgaatttcc agacggtcgt
    14701 taaccctcac cgcgcggcta atccagaact ttgcagtttc cagaagtagc gccatgcctt
    14761 catgcgcaat gaaactttca tcccccgtgg tctgccagta ttgaataacc gcccaggcga
    14821 tatcggccac cagatgatgt tccgcctgcg ccgaggccac tttttgccgc agcccggtgc
    14881 gaatgttaat ggcggcaaat tccggcgtct cttcttcgcc gctgcgcgcg ctttcccacg
    14941 gaaatagcgc gccctgccag ccgttgcgtc gcgctttctc ctgcgcgcct ggcaagttgt
    15001 gccagcgata acgcagtaaa cttcgggcaa ccgtcggatc gctaaacaga tggaacggca
    15061 gcaaaaatac ttctgtatcc cagaaaacgt ggcctttgta gccttcgccg gttaagcctt
    15121 ttgccgcaat actgctgcgc tcgccgtgag ccggcgtcat gatgcgcaga tgataaagcg
    15181 cataatctaa cgcttgctga tcgtgcgcat cgccgccatt taccgtgata cgacgtttct
    15241 gccaccattg acgccagttt tctgttgatg ctgcaagatg ttggtcgtaa ctctgttgcg
    15301 cgcacatttc aagctggcga agcgacacgc tgccccactc gtctaaagca gcttgcctgt
    15361 catcccgcca gtcgatccac accagttttt gcaacgtcac tgtctcgcct gcatgaagct
    15421 gcgtactggt atgttgcagt aaacggcgct ctttggcggt atagcattgc tgcacatcac
    15481 cgctcacctg gcagcaacaa ctgatggcca catcactgcg cccatcctgg gtggtgtaga
    15541 tcccctgcat caaatgctga ccaaacaccc gcacctgggt ttcgtcgaga tgctggcgac
    15601 cgtggttggt ttgcgtggcg tcaatgcctg ttgaaatcag cactgacgcg tcggcgtcca
    15661 gtggcataat agtgatttcc agcgcaatga gcggcagttg gtctgccgaa acaaagcgac
    15721 ggctggcgat ggtgtaacct gcgccgttgc tggtacgcca gacaacgcta cggcgtaatt
    15781 ctccactggc aaagtcaagt tcacgctgcc aggcttcgcg ggataacgag aaaacctcac
    15841 catttatggc aatctccatc cccaagatat caggcagatt caccagttcg ttgatttcac
    15901 cttttcccgc ccgatgatac agccccgcca gatacatccc tcgcgtctgg cgcgtgtaat
    15961 cttcttcatg gctggcgcga agcccaagat agccgttccc ctgcgccatc agcgatgcat
    16021 acttgttcag ggtatgctgg ctgaaatggg gttctgataa cgttactggc ctggtcataa
    16081 ttccacacgc gtccctgttt ctgcggattg atataacgcc gccaccagtt gctggatgat
    16141 gtacccctgc tcggcgtcgg ctatcatcac gggcttgccc tgtacgtgat tgataaaggc
    16201 ttccatgctg cgcaaatggc ggttgtcgtc tgccatttcc cgttgcatca gcgtcattaa
    16261 ttcaccgttg ttatcggtgt agatatgtgc tggaaacagc gtcgcaccgg ctttatcacc
    16321 acaaaagctg acgttcataa tcaactgttc gcggatgttg agtgcaaatg acgtttccag
    16381 ccacagaatg ccgccgttat gaaattcaat ggtgccaaac agcgaatctt cgacgctgta
    16441 agttgccgga tcccactcgc caaattgacc acagctcttt tgcgtgccga tcttttgaaa
    16501 actatgcgca ttcacgcttt tcaccgccgg aaagcccagc acatacatcg cagcatccag
    16561 catatgaatg ccgatgtcga tcagcaggcc accaccctgc agttctttat tggtaaagac
    16621 accccagccg ggaacgccgc agcgacgcag ggcgcgggcg gtggtaacgt aaatctctcc
    16681 caaaacgccg ttggtcacct gttcacgcag ctgttgcgta tcgagcgcaa acgatggtga
    16741 aagtcgtagg ccagcacctt gcccagtttg cgcgcggtat cgcacatttc ccacgcctgt
    16801 tctgtcgtca tggcgggcgg tttttcgcac atcacatggc agcctgcttc cagtgccatc
    16861 agggtatgtt cgtaatgaaa acggttaggt gagccgacgc taaccacatc aggtttcacc
    16921 gccagcagca tggcctgcgg atcgtcccac acggatgcat tcccgtattt ttctgccagc
    16981 gcctgcgcct gggaaaggcg gctgtcacag acagccacca gttccagatc gttgcgggtg
    17041 cagtagtacg aagcatgaac cttatccgcc acctggcccg cgcctattat cgcgacccgc
    17101 agcggagagc ttgtcattgc acttttcaca aagacctctt agcaggtacg caaccaggcc
    17161 aacgaatcac ggtacgcctg ggcaggatct tctgcccgga tacgcccttc atacaccaca
    17221 taaccctgat agttatcggc gcgcagctgt tcaaacagcg cgtggaaatc gagggtgcca
    17281 ctgcccggct ggtaacgatg gttatcggca atatgcacat gaccgagcaa gtcacggttg
    17341 tcgtgcagcg cctgcgccag gttatcttct tcgatgttca tgtgatagaa atcgccgata
    17401 atctgcacat gtttaagatt gttttcgacg atgtaacgac gagcatcggc gagggtgttg
    17461 atcatatgat cctgatagcg gtttaacggt tcgagataca ccacggttcc ggtacgcgcg
    17521 gcgacctgtt ccagtacgcg cagggaatca ctcaccagtt tgcggtcgcc gtccaggcta
    17581 cgcggcgagg tcatcggcgg taagcggaag gtaaacatgc cccacgctgc cggaaacccc
    17641 ccacttctgc cagcgcctcg agaatgcgtt cgatctgctt taagccatta agacgacgct
    17701 cttcgataaa gtcaccaatc cagccgtcat agccgccgca ggcggtggtc actggtaaac
    17761 cggtttcttt gatcgccgct ttgacttctt cgatgttgtt gaccagcagt ttgccgtcaa
    17821 tctcaaaacc atcgaagccc atctctttga tataacgaaa tctctcgcgg atattttccg
    17881 gaaagaatgc ctgattctgt gtgccgattt tcattttttc attcctttga attaaaacgt
    17941 aacgcccatt ttgatgctct gttccggatg ctgatcgaca tactgcatgt agctttccgg
    18001 gctgttggca aaggtcacta ccgggtcgat taaatcttcg caattgagat aaccgttcat
    18061 cagcagttcc cagcaggttt cttcaatacg cttacggctc cagcgcggat aatccgggtt
    18121 cggttcgctg cacgcgcggg agaaaacaat tttggcgtta ttgaaatgcg cttcgcgtcc
    18181 gaggttaaaa ccttcagcaa atggtttggc aaacgcgaca taggagatgg tgccgccgta
    18241 ggccaggccg cgcagcgccg attgcagcgc gtcggcgtaa ccgctggttt cgataatcac
    18301 atcagccccc tgcttgccgg tcagcgtttt gatctctttg cctacgtcag tgccaatggg
    18361 gttaaggcag aaatcagcac cgtggcgacg ggcaatatca cagcgatggg cgataggatc
    18421 gacgccaatt accaccgatg cgccagcgcg tttcgccagt tggatggcaa tttgaccgat
    18481 cgcgccaagc cctaccacca cgacaaaatc ccctacgcgt acgttggcat cgcgcacgcc
    18541 gctcatggca aactgcgccg ggtcgtaaca gacggcgttt ttccaggagc tgccttgcgg
    18601 cattttgcgc agcttgtagt tattcactgc gttaatgatg accgtctcgg agagcgggcc
    18661 gtagccgcat acgctgtcgc ccaccgcgta gtctgtaacg tcgctgccgc actcgataat
    18721 gtcgccaacc accatgttgc caagctggaa tttgccaaac tcaatgccgc gcggcgcatc
    18781 tgccggacgc ggggtgaaca tctgccactc gccgttaaag tcttcatcga taaacgggct
    18841 ggcggcgcgg aagtcgacca cttccgttcc gtgtttcggt gcgccgaaac gcacgcggat
    18901 cttcacttca ttggctaaaa tcgcccgatc ttcatactca accagcgcag caacacgcgg
    18961 tgctgtggct actaactttt tcatgtaaaa ctccttgtaa agaaactggc cgctttagcc
    19021 cttcacgcca ccggcggtca aaccactctt aataaaacgt tccgaaagcg catacataat
    19081 caccaccggc aatgcggtca ccagcgaggc cgccatcatc cgtccccaga tgtaatcagg
    19141 cgtactgaac agcgcgttca ggcccactgg taaggtgaaa ttgctggcgc tggagaggaa
    19201 aatcgacgca aacagatagt cgttccacgc caccataaag cagtagacga acaccgatat
    19261 cagcccggac atcgccaggg gcacggtgat acggaagatg atttgcagac ggttgaggcc
    19321 atccatcatc gccgcttctt cgatctcatc agggatggtg tcgaagtaac ttttcagcat
    19381 aaacacggcg gtgggcaggg tttgcgtcac catggtaatg atcagcgcca tctcggtgtc
    19441 gtaaatacca agcgcggtga tgattttgaa cagaggcacc accagcaaaa tgccagagaa
    19501 catatacacc gtgtaaaagc tggcgttgat ggtcatccgg cctttaaagc gcaggcggga
    19561 aagcgcgtaa gctccgagaa tgccgagaaa taccgccacc acggaggaaa ccaccgacac
    19621 caccatgctg ttacggaagt agtcgacaaa cgggaaaatc atcgggttaa aaatgtcgac
    19681 gtaatgctcc agcgtccact gttgcggcag cagcgtagga tgcagcgata tcgcctcttt
    19741 cgcgcttttg aacgaggtca tcagcatcac aaaaaacgga aacagcgtga tgatgagaaa
    19801 cagcgccagc ccgcagtaaa aaccgatacg actgagcgtg cgtttatttg ttgccattga
    19861 ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag agcaccaccg
    19921 agatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag agatagacac
    19981 caagaatatc gaccttggtt gtcagcagat aaacatcagc gaacatgtag aacatccaga
    20041 tggtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc gtgacgatgc
    20101 gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc gatttgtcga
    20161 tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc cagatggcga
    20221 acagcaccac cagcacaaag ctactgcccg gattatcaaa ccacagcggt gcctgctcat
    20281 agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg ttgaacatgt
    20341 atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc accagcgaac
    20401 gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccacggcc agcccgagca
    20461 cggtgctgcc cgccaccacc agcgccgtat accagaccgt catccacagc gagtgccaga
    20521 agccgggatc ggagagaatg cgcacatagt tactcacccc aacaaacgtt gactcgatgt
    20581 tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatc ggccacgcca
    20641 ccagaccgcc cagcagtaat aagctgggcg cgagaagcag cagcgcaaac ggcatatcgg
    20701 aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt gctgttcgat
    20761 cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg ctttacccac
    20821 ggtgacgtta tgcaccatgg agctcaccac accagaaccc gtcacatcgc ccatgcgggt
    20881 aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac cgattagctg
    20941 attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg cggtagtgac
    21001 aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt cggcaatgtt
    21061 gtccgcctgc tccataaagg tgacaaattt ttctgctgct tcggtctttt gtccggcggt
    21121 aatggtcagc gaggtcaaca tgccgtagac cgcagagttt ttctcggttg gcaccacgaa
    21181 accgacgttt ttcgggtcgc cttctttaat cacagccgga aggatatagg tggagtaaat
    21241 cgccatcggc gcggtgccgt tcataaaggc gtctttcact tccatgatgt cgttagaacc
    21301 cggcatggtg ttggcagcaa ggtcgcggta ataggtcagc gcctgcatca tctctggtgt
    21361 atcaagggtg attttgcctt cggcgttaaa gacgttggcc tagttggata acgcaaactg
    21421 ggagaaggat tgttccgtca gcacgctttc tgccgtaggc agcgcaatac cgtatttttt
    21481 attcgccggg tcattcagtt tctgtgcaac gtccagcagc tgttgccagt ttttcggctc
    21541 ctccagcccc gcttttgcca gcacatcttt gcgataccag atgtcgccaa tccaggcgct
    21601 gacaggaaca ccggtccatg cgctaccatc ttcggtacgc acaatacgca gtacgccatc
    21661 gtaaaacgcg ccttcaccaa cgttgctgat gaccgtggca accgctttgc gatcgataag
    21721 ctgctcttta tccataactt tggcgtagtc atggctggtt tcgatcactt ccggcagcga
    21781 gccgctacgt gaaagggtga tgactttggt gttatgagcg tcttcttcca cgggcacttg
    21841 tttaacggtg atacctgggt tttctttttc aaaacgggca atcaatttgc tgataactgc
    21901 ctgacgctcc tgctccaccg aagaatgcat aaattcgatg gatacatggg ttttattttc
    21961 ttctttacag cctgaaatca gggcgcagga aaccagtgct gataacagca cgatttttga
    22021 tttaatcatg agatgttcct ttttatttaa tccacataac ctgccagggg cgcagcgtta
    22081 tttcattacc caatatatca acttcactaa tcagatcccg accatgcaga tcagtaatat
    22141 ttacatgctg aatattttta ctgacattaa acaatccagt cagacaatta ccatcagcgt
    22201 tacttctttg aatacgcatt acggatgaat taaccgtatc gatggtaaaa ttattatccg
    22261 gatgaaactc gttatggctg cgacgaagtg taattaaacg cgacaactca tgatataccg
    22321 catgccttaa tgtagcttca tcgttcagtt ctcgtgttat ctcttcactg tgatattttt
    22381 tacggttaat cgcacggtta tatccgagtt tttcgacacc tgcgtaatca ttacgcgagc
    22441 caagaatact ttgaatatat atcgcgggta caccggggaa acttaacaaa atcgcatggg
    22501 caaggataaa cctggtgcaa cgttcttcat cgctactctc gcggcggctt aacgcattca
    22561 tataggtgac attcatctca tagggactgc gcgtgccgtc gggattattt ttccagttta
    22621 cta
//
LOCUS       DAJVRL010000063        22106 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVRL010000063 DAJVRL010000000
VERSION     DAJVRL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.063"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OQN02_002470"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="OQN02_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS1643221.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="OQN02_002471"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="OQN02_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1643222.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="OQN02_002472"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="OQN02_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1643223.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OQN02_002473"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="OQN02_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1643224.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="OQN02_002474"
     CDS             3251..5011
                     /locus_tag="OQN02_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1643225.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="OQN02_002475"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="OQN02_002475"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1643226.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OQN02_002476"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="OQN02_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1643227.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OQN02_002477"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="OQN02_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1643228.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OQN02_002478"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="OQN02_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1643229.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OQN02_002479"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="OQN02_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1643230.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="OQN02_002480"
     CDS             complement(9679..11586)
                     /locus_tag="OQN02_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1643231.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OQN02_002481"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="OQN02_002481"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1643232.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="OQN02_002482"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="OQN02_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1643233.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OQN02_002483"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="OQN02_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1643234.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OQN02_002484"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="OQN02_002484"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1643235.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="OQN02_002485"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="OQN02_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="OQN02_002486"
     CDS             complement(17598..18113)
                     /locus_tag="OQN02_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1643236.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="OQN02_002487"
     CDS             complement(18121..18663)
                     /locus_tag="OQN02_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1643237.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18761..19744)
                     /locus_tag="OQN02_002488"
     CDS             complement(18761..19744)
                     /locus_tag="OQN02_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1643238.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19735..>22106)
                     /locus_tag="OQN02_002489"
     CDS             complement(19735..>22106)
                     /locus_tag="OQN02_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCS1643239.1"
                     /translation="VLNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAFPA
                     FKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGINA
                     LFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSYL
                     QRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKSN
                     AQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSVP
                     LLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAAM
                     FGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRYS
                     TSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYGL
                     FYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALNFSL
                     PLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQQGY
                     NSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLGQSL
                     GETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNVDLE
                     NAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDD
                     GSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
BASE COUNT         5479 a         5794 c         5194 g         5639 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtggac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaacaa
//
LOCUS       DAJVRL010000064        21588 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVRL010000064 DAJVRL010000000
VERSION     DAJVRL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.064"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..138
                     /locus_tag="OQN02_002490"
     CDS             <1..138
                     /locus_tag="OQN02_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1643240.1"
                     /translation="SSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFTTHRIK
                     D"
     gene            complement(177..524)
                     /gene="grxD"
                     /locus_tag="OQN02_002491"
     CDS             complement(177..524)
                     /gene="grxD"
                     /locus_tag="OQN02_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS1643241.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            859..1686
                     /gene="mepH"
                     /locus_tag="OQN02_002492"
     CDS             859..1686
                     /gene="mepH"
                     /locus_tag="OQN02_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1643242.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1814..2395
                     /gene="sodB"
                     /locus_tag="OQN02_002493"
     CDS             1814..2395
                     /gene="sodB"
                     /locus_tag="OQN02_002493"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1643243.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2541..3710)
                     /locus_tag="OQN02_002494"
     CDS             complement(2541..3710)
                     /locus_tag="OQN02_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1643244.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3876..3965)
                     /gene="ynhF"
                     /locus_tag="OQN02_002495"
     CDS             complement(3876..3965)
                     /gene="ynhF"
                     /locus_tag="OQN02_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1643245.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4264..5289
                     /gene="purR"
                     /locus_tag="OQN02_002496"
     CDS             4264..5289
                     /gene="purR"
                     /locus_tag="OQN02_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1643246.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5286..6218)
                     /locus_tag="OQN02_002497"
     CDS             complement(5286..6218)
                     /locus_tag="OQN02_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1643247.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6331..7542
                     /locus_tag="OQN02_002498"
     CDS             6331..7542
                     /locus_tag="OQN02_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1643248.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7833..8981
                     /gene="cfa"
                     /locus_tag="OQN02_002499"
     CDS             7833..8981
                     /gene="cfa"
                     /locus_tag="OQN02_002499"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1643249.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9021..9662)
                     /gene="ribE"
                     /locus_tag="OQN02_002500"
     CDS             complement(9021..9662)
                     /gene="ribE"
                     /locus_tag="OQN02_002500"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1643250.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9877..11250
                     /gene="mdtK"
                     /locus_tag="OQN02_002501"
     CDS             9877..11250
                     /gene="mdtK"
                     /locus_tag="OQN02_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1643251.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11291..12547)
                     /locus_tag="OQN02_002502"
     CDS             complement(11291..12547)
                     /locus_tag="OQN02_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1643252.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12855..12931
                     /locus_tag="OQN02_002503"
     tRNA            12855..12931
                     /locus_tag="OQN02_002503"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12889..12891,aa:Val,seq:gac)
     gene            12936..13012
                     /locus_tag="OQN02_002504"
     tRNA            12936..13012
                     /locus_tag="OQN02_002504"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12970..12972,aa:Val,seq:gac)
     gene            13120..13425
                     /locus_tag="OQN02_002505"
     CDS             13120..13425
                     /locus_tag="OQN02_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1643253.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13551..15155
                     /locus_tag="OQN02_002506"
     CDS             13551..15155
                     /locus_tag="OQN02_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS1643254.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(15167..15979)
                     /locus_tag="OQN02_002507"
     CDS             complement(15167..15979)
                     /locus_tag="OQN02_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643255.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15983..16768)
                     /locus_tag="OQN02_002508"
     CDS             complement(15983..16768)
                     /locus_tag="OQN02_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS1643256.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16765..17433)
                     /locus_tag="OQN02_002509"
     CDS             complement(16765..17433)
                     /locus_tag="OQN02_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1643257.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17497..18135)
                     /locus_tag="OQN02_002510"
     CDS             complement(17497..18135)
                     /locus_tag="OQN02_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS1643258.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18148..20249)
                     /locus_tag="OQN02_002511"
                     /pseudo
     CDS             complement(18148..20249)
                     /locus_tag="OQN02_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20270..20896)
                     /locus_tag="OQN02_002512"
     CDS             complement(20270..20896)
                     /locus_tag="OQN02_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS1643259.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(21352..21561)
                     /gene="fumD"
                     /locus_tag="OQN02_002513"
     CDS             complement(21352..21561)
                     /gene="fumD"
                     /locus_tag="OQN02_002513"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS1643260.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5399 a         5600 c         5014 g         5575 t
ORIGIN      
        1 agtagctgcg aaggtgcgcc aatatccgtc atccgccagt acattgaaca acagcaaaca
       61 cctggtcagg tggaaaaccg cgccttatat ccccgccctg aaggacgagg ttttacgacg
      121 caccggataa aggactgacg gatgcccgtt cgcatccgtc agtattgcag gacggattat
      181 tccgcgtccg gctcttcaga cttgtattta gcggcagttt ctttgatcag ctgctgcagt
      241 tcgccacgct gatacatttc gatcacgata tcacaaccgc cgaccagctc gccgtcaacc
      301 cacagttgcg ggaaggtcgg ccagttagca tatttcggca gttccgcacg aatgtccgga
      361 ttctgcagaa tatcaacata ggcaaaacgt tcgccacatg cggcaagcgc ctggactgcc
      421 tgggcagaga aaccgcagct cggcagtttc ggtgaacctt tcatgtacag caggatcggg
      481 ttttcagcaa tctggcgttg gattttttcg atagtggtgc tcattgtctt gcttcctcaa
      541 acttctttac ggcagtaatc tgacattgta gcgggtcagt gcggcatcgg aaaataacat
      601 tttcatcacg cttttgctat tttatccctt tgctctattt gttgcatttc aaatattcgt
      661 ttcttctctg atgcgcggca aactggcgta tttataacca tttttagctt tcactgctgc
      721 atttttttgc gctcgccaac gaaacgtatt ttttaacaat aaaagctatt aactttctct
      781 tcttctatgc attagaatca tcaagttttg taaatcagac gcaggcatga tagacctgcc
      841 ttaacagagg gacgctcagt ggcgcggata aaccgtattt cgatcacgct ctgtgcttta
      901 ctttttacca ccctgccttt aacgcctatg gcccatgctt caaagcaagc cagggagagt
      961 tctgctacca ctcatatcac caaaaaagca gataaaaaga aaagcacggc aaccaccaaa
     1021 aagacccaga aagccagcca gaaaacagcg aaaaaagccg ccagtaaaag tatgaccaaa
     1081 agcaaaaccg cttcttctgt taaaaaatct tccattaccg cttctaaaaa cgccaaaact
     1141 cgcagcaaac acgccgtcaa taaaacggcc tcagccagct ttaccgaaaa gtgtaccaaa
     1201 cgtaagggct ataaatcgca ttgtgtgaaa gtcaaaaatg ccgcgtcagg aacccttgcc
     1261 gacgcgcaca aagcgaaggt gcaaaaagcc acgaaagtgg caatgaataa actgatgcag
     1321 caaattggta agccatatcg ttggggcggc agctcaccgc ataccggttt tgattgcagc
     1381 ggcctggttt attacgctta taaagatttg gtgaaaattc gtattccgcg tacggcgaat
     1441 gaaatgtatc acctgcgtga tgcaggacct atcgaacgta gtgagctgaa aaacggcgac
     1501 ctggtctttt tccgtactca gggacgcggt acagccgatc atgtcggcgt gtatgtcggc
     1561 aacggcaaat ttattcagtc accgcgcaca ggtcaggaaa ttcaaatcac ttctctcagt
     1621 gaagactact ggcagcgcca ctatgttggc gctcgtcggg taatgacccc aaaaacactt
     1681 cgctaaaact ttaccctgtt gttacggcaa cagggtaagt tcatcttttg tctcaccttt
     1741 taatttgcta ccctatccat acgcacaata aggctattgt acgtatgcaa attaataata
     1801 aaggagagta gcaatgtcat tcgaattacc tgcactacca tatgctaaag atgctctggc
     1861 accgcacatt tctgcggaaa ctatcgagta tcactacggc aagcatcatc agacttatgt
     1921 cactaacctg aacaacctga ttaaaggtac cgcgtttgaa ggtaaatcac tggaagagat
     1981 tattcgcagc tctgaaggtg gcgtattcaa caacgcagct caggtctgga accatacttt
     2041 ctactggaac tgcctggcac cgaacgccgg tggcgaaccg actggaaaag tcgctgaagc
     2101 tatcgccgca tcttttggca gctttgccga tttcaaagcg cagtttactg atgcagcgat
     2161 caaaaacttt ggttctggct ggacctggct ggtgaaaaac agcgatggca aactggctat
     2221 cgtttcaacc tctaacgcgg gtactccgct gaccaccgat gcgactccgc tgctgaccgt
     2281 tgatgtctgg gaacacgctt attacatcga ctatcgcaat gcacgtcctg gctatctgga
     2341 gcacttctgg gcgctggtga actgggaatt cgtagcgaaa aatctcgctg cataataact
     2401 gatggcaaat gcagcattgc ctgatgcgct tcgcttacca tgccaacgcg gatgaggcgt
     2461 taacagcgca ttcggcaaga taaatcgcac gttgtcagca actgtaacgc agaaggttat
     2521 ccttctgcgt ttttgtttaa ttagctgtta gcaacgcaaa ctgtttcagg ttgttttctg
     2581 gctgacataa acaccagcaa taatgccagt cccgcgacaa tcgcccccgt caccggcaca
     2641 aagctgtatc ccagcccagc ggaaattacc gcaccaccag cagctgctcc cagcgcattt
     2701 ccaagattaa aggcaccaat attgactgat gaagacagac ctggcgcttc actggcgaca
     2761 tgcatcacgc gcatctgtaa cggaggtaca accgcaaagg ttgctgcgcc ccacaccacc
     2821 atgctaatag ctgcgccgaa ctcattgcgg gccaggaacg ggattgccag cataatcacc
     2881 atcagcagca acaaaaagcc tttcaacgtg ccgttaactg aacgatctgc cagtttgccg
     2941 ccgagatagt taccgataga gaatccgaca ccaatcagca ccagcattgc cgtgacgaac
     3001 accggtgttg cgtgggtaat actttgcagt accggagaga tataggtgta gagggtaaac
     3061 attgcaccag ctcccagtac cgtcgtcagc aatgcagaca gcacctgcgg acgcattaat
     3121 accgccagct cttttttcac ttcaggtcgt gcccctgtac cacctttagg taatgagaag
     3181 aacagactta ccattgaaat cactcccagc cccgccgttg ccagaaatga catccgccag
     3241 ccgatggttt cacccaacca ggtcgccgcc ggcacgccac cgatatttgc cagggttaac
     3301 cccataaaca tagtggcaac tgcgctggcc tgtttatgtt ttggcaccac gcttgcggcc
     3361 acgactgaac ccaaaccaaa aaatgctccg tgattcaggc tggtcaaaat acgtgaaagc
     3421 atcagggtca tataatccgg cgcgatggcg gaaagaacgt tgcctagcgt gaaaattgcc
     3481 atcaggaaaa tcaacgcact gcggcgggca cgatgagaaa gtagaagcgt catcagcggc
     3541 gcgcccacca ttacgccaac tgcataggca ctgattaaca ttccggcagc gggaatcgag
     3601 acatccacac cgcgcgcaat gacgggcaac aagcccattg gcgagaactc cgttgtcccg
     3661 ataccaaacg cgccaatcgc cagcgccagc aacggatagt taattttcat gccttatctc
     3721 cacctcttcg cgtcattacg cgatattcat taaagtggcg aaagcatgac agcaatcaca
     3781 aaaaaatgaa aataacaaaa agagaaaaca cttttgccat tttgctaaca aacaggaagg
     3841 agatgcgagg gagaacgcgc tccctcgaga ggaaatcagt gcagcgcggc agtcaaaccc
     3901 acggctacga tcaaaccgag gacgataatc gttgttacca gtgaaaattt aaggtcggtg
     3961 ctcatcaagt tttctccttt tttattacca cacaaaaagt gatattacgc atttttacac
     4021 actgtgatga aaaaatctcc cgtcatttat aatgataagt gtttttacca cttccccttt
     4081 tcgtcaagat cggccaaaat tccacgctta cactatttgc gtactggcca ttgacccctt
     4141 cctgacgctc cgtgtcgttt ttccggcgta ccgcaacact tttgttgtgc gtaaggtgtg
     4201 taaaggcaaa cgtttacctt gcgattttgc aggagctgaa gttagggtct ggagtgaaat
     4261 ggaatggcaa caataaaaga tgtagcgaaa cgagcaaacg tttccactac aactgtgtca
     4321 cacgtgatca acaaaacacg tttcgtcgct gaagaaacgc gcaacgccgt gtgggcagcg
     4381 attaaagaat tacactactc ccctagcgcg gtggcgcgta gcctgaaggt taaccacacc
     4441 aagtctatcg gtttgctggc gaccagcagc gaagcggcct attttgccga gatcattgaa
     4501 gcagttgaaa aaaattgctt ccagaaaggt tacaccctga ttctgggtaa tgcgtggaac
     4561 aatcttgaga aacagcgggc ttatctgtcg atgatggcgc aaaaacgcgt cgatggtctg
     4621 ctggtgatgt gttctgagta cccagagccg ttgctggcga tgctggaaga gtatcgccat
     4681 atcccaatgg tggtgatgga ctggggtgaa gcaaaagctg acttcaccga tgcggtcatt
     4741 gataacgcgt tcgaaggcgg ctacatggcc gggcgttatc tgattgaacg cggtcaccgc
     4801 gaaatcggcg tcatccccgg cccgctggaa cgtaacaccg gcgcaggccg ccttgccggt
     4861 tttatgaagg cgatggaaga agcgatgatc aaggtgccgg aaagctggat tgtgcagggt
     4921 gactttgaac ctgaatccgg ttatcgcgcc atgcagcaaa tcctgtcgca gccgcatcgc
     4981 cctactgccg tcttctgtgg tggcgatatc atggcaatgg gcgcactttg tgctgctgat
     5041 gaaatgggcc tgcgcgtccc gcaggatgtt tcgctgatcg gttatgataa cgtgcgcaac
     5101 gcgcgctatt ttacgccggc gctgaccacg atccatcagc caaaagattc gctgggtgaa
     5161 acagcgttca acatgctgtt ggatcgtatc gtcaacaaac gtgaagaacc gcagtccatt
     5221 gaagtgcatc cgcgtttgat tgaacgccgc tccgtggctg acggcccgtt ccgcgactat
     5281 cgtcgttaat cacccgttgc gggagtctct tccggctccc gtagccactc cttattcagc
     5341 gtctcactat cgccgagata ctcaagcagc caggttaacg caggcgacat atcattttgc
     5401 tgccatgtca gacaacatgc cgaatccggg aaggggtttt ccagttcaag tgctacccac
     5461 ttcccctcat taagccacgg tttggcgaaa tgtgttggca ccatccctat gcataatcct
     5521 gccgagatac aggttgccga tgattcccag tcaggtacga caagtctttt ttggttatcc
     5581 agcaaccagg taatacgttt aggtagcgtt cgcgaggtgt cttcgcgcac caacgacggc
     5641 cagttgcgca acgtatcatc gctgaacggg ccatccatca acgccagcgg gtggtgactg
     5701 gcaacaacgc aactccagct tagcatcccc atatcccgga aggcataacg accgcctacc
     5761 ggaatcgcgc gtgttgcgcc aattgccagt tccacgcgcc cgtcggaaag cgcatcccag
     5821 acaccgttga acacttcctg aaagacaaga agttcgacat catcaaaatg gcgataaaaa
     5881 tcaacgatca tctgccgtgt acgttctggc ctgacaatat tatccactgc gatagctaac
     5941 tgaccgcgcc agccgttcgc tatctgctga cattgctggc gggtgatctg catttttttg
     6001 acaacagagc gcccttcttt gagaaaccac gctccagcag cggtcagctc cacatcacgg
     6061 tgccgtcgtt caaagagcgg taccgccagc cactcttcca gctgacgcac ggtatagctg
     6121 accgcagaag gaacacgatg cagctcctgt gccgcagcgc taaaactacc attacgcgct
     6181 accgcatcaa caacttcgag tgaatattct gaccacatag tctgcctgca aaatttttga
     6241 aaccagtcat caaatattac cgtttcacaa cactaatttc actccctaca ctttgcggcg
     6301 gtgtttaatt gagagattta gagaatatac atgcaacctg ggaaaagatt tttagtctgg
     6361 ctggcgggtt tgagcgtact cggttttctg gcaaccgata tgtatctgcc tgctttcgcc
     6421 gccatacagg ccgacctgca aacgcctgcg tctgctgtca gtgccagcct tagtctgttc
     6481 cttgccggtt ttgccgcagc ccagcttctg tgggggccac tctccgaccg ttatggtcgt
     6541 aaaccggtgt tattaatcgg cctgacaatt tttgcgttag gtagtctggg gatgctgtgg
     6601 gtagaaaacg ccgccacgct gctggtattg cgttttgtac aggctgtggg ggtctgcgcc
     6661 gcggcggtta tctggcaagc attagtgaca gattattatc cttcacagaa agtcaaccgt
     6721 atttttgcga ccatcatgcc gctggtgggt ctgtctccgg cactggctcc cctgttagga
     6781 agctggctgc tggtccattt ttcctggcag gcgattttcg ccaccctgtt tgccattacc
     6841 gtggtgctga ttctgcctat tttctggctc aaacccacga cgaaggcccg taacaatagt
     6901 caggatggtc tgacctttac cgacctgcta cgttctaaaa cctatcgcgg caacgtgctg
     6961 atatacgcag cctgttcagc cagttttttt gcatggctga ccggttcacc gttcatcctt
     7021 agtgaaatgg gctacagccc ggcagttatt ggtttaagtt atgtcccgca aactatcgcg
     7081 tttctgattg gtggttatgg ctgtcgcgcc gcactgcaga aatggcaagg caagcagtta
     7141 ttaccgtggt tgctggtgct gtttgctgtc agcgtcattg cgacctgggc tgcgggcttc
     7201 attagccatg tgtcgctggt cgaaatcctg atcccattct gtgtgatggc gattgccaat
     7261 ggcgcgatct accctattgt tgtcgcccag gcgctgcgtc ccttcccaca tgcaaccggt
     7321 cgcgccgcag cgttgcagaa cactctgcaa ctgggtctgt gcttcctcgc aagtctggta
     7381 gtttcctggc ttattagtat cagcacgcca ttgctcacca ccaccagcgt gatgttatca
     7441 acagtagtgc tggtcgcgct gggttacatg atgcaacgtt gtgaagaagt tgactgcccg
     7501 aatcatggca atgccgaagt cgctcatagc gaatcacact gatctatatc gatatactta
     7561 tacttaggct gctaacaaaa ttttgttgta tgattgaaat tagcggccta tactaatttc
     7621 gagttgttaa agctacgata aatattatgt ttttacgggg acaggatcgt tcccgactca
     7681 ctatggatgg tcatttcggc aagggttcct cctttccctc tgttctacgt cggattatag
     7741 actcgcggtt ttttctgcga gatttctcac aaagcccaaa aagcgtctac gctgttttaa
     7801 ggttctgatc accgaccagt gatggagaaa ctatgagttc atcgtgtata gaagaagtca
     7861 gtgtaccgga tgacaactgg taccgtatcg ccaacgaatt acttagccgt gccggtatag
     7921 ccattaacgg ttctgccccg gcggatattc gtgtgaaaaa ccccgatttt tttaaacgcg
     7981 ttctgcagga aggctctttg gggttaggcg aaagttatat ggatggctgg tgggaatgtg
     8041 accgactgga tatgtttttt agcaaagtct tacgcgcagg tctcgagaac caactccccc
     8101 atcatttcaa agacacgctg cgtattgccg gcgctcgtct cttcaatctg cagagtaaaa
     8161 aacgtgcctg gatagtcggc aaggagcatt acgatttggg taatgacttg ttcagccgca
     8221 tgcttgatcc cttcatgcaa tattcctgcg cttactggaa agatgccgat aatctggaat
     8281 ctgcccagca ggcgaagctc aaaatgattt gtgaaaaatt gcagttaaaa ccagggatgc
     8341 gcgtactgga tattggctgc ggctggggcg gactagcgca ctacatggca tctaactatg
     8401 acgtaagcgt ggtcggcgtc accatttctg ccgaacagca aaaaatggct caggaacgct
     8461 gtaaaggcct ggatgtcacc attttgctgc aagattatcg tgacctgaac gaccagtttg
     8521 atcgtattgt ttctgtgggg atgttcgagc acgtcggacc gaaaaattac gatacctatt
     8581 ttgcggtggt ggatcgtaat ttgaaaccgg aaggtatatt cctgctccat actatcggtt
     8641 cgaaaaaaac cgatctgaat gttgatccct ggattaataa atatattttt ccgaacggtt
     8701 gcctgccctc tgtacgccag attgctcaat ccagcgaacc ccactttgtg atggaagact
     8761 ggcataactt cggtgctgat tacgatacta cgttgatggc gtggtatgaa cgattcctcg
     8821 ccgcatggcc agaaattgcg gataactaca gtgaacgctt taaacggatg tttacctatt
     8881 atctgaatgc ctgcgcaggt gctttccgcg cccgtgatat tcagctctgg caggtggtgt
     8941 tctcacgcgg tgttgaaaac ggccttcgag tcgctcgcta aaggctattc tatcgccccc
     9001 tcttcggggg cgagttcaga tcaggcttct gtacctggtt gattcatggc attttctcgt
     9061 gccgccagca cacgttctac cgtatctacc actgcctgag tttgtggatc gatttcaatg
     9121 ttgacgcgtg cgccaagttt tttcttccca agagtcgtgc gttccagtgt ttccggaatt
     9181 aaatggacgc aaaaacgcgt tggcgtgact tcgccgacgg tcaggctaat accgtcgatg
     9241 ccaataaatc ctttgtacag gatatatttc atcaactgac tatcctggac tttaaaccag
     9301 atctggcgat tattttccga ggttaatatt ttcgccactt cagcggtggt cataatatga
     9361 cctgacatta agtgtccgcc aatttcatca ctgaatttcg ccgcacgctc aacgtttacc
     9421 caatccccca cttttaaatc gccaagattg gtaatgcgta acgtttcttt catcaggtca
     9481 aaactgacat ggttgccgtt aatttccgtc acggtcaggc agcaaccgtt atgcgccacg
     9541 gaagcaccgg tttccaggcc gtccagcatg tggtcgggta actccaccac atgcgtacga
     9601 aaatttggtt tctcgtcaat cgacaccagt tttgcggtgc cctgtacaat ccccgtaaac
     9661 atacttacaa ctcctgaaat cagttaagac attctgttca gcacaatagc aggtggaaaa
     9721 cgcccttacc agtgaagggg taagaatggc tattttttca ctggagaatt aataaatcct
     9781 cgctacaata gactgaattt cccctgcttc ttctttttgc tgcccattca ggcggctttt
     9841 tagtctctca tataactaca aataaaaggt gttcacgtgc agaagtatat cagtgaagcg
     9901 cgtctgttat tagcattagc aatcccggtg attctcgcgc aaatcgccca aactgcgatg
     9961 ggttttgtcg ataccgtgat ggcgggcggc tatagtgcca ccgacatggc ggcggtcgct
    10021 atcggtactt ctatctggct tccggcgatc ctctttggtc acggactgct gctggcatta
    10081 acgccggtta tcgcgcaatt aaatggttcc ggtcgacgtg agcgcattgc gcatcaggtg
    10141 cgacaaggtt tctggctggc aggttttgtt tccgttctca ttatgctggt gctgtggaat
    10201 gcaggttaca ttatccgcta catggaaaac atcgatccgg ctctggcgga caaagccgtg
    10261 ggttatctgc gtgcgttgtt gtggggcgcg ccgggatatc tgttcttcca ggttgcccgt
    10321 aaccaatgtg aaggtctggc aaaaaccaag ccgggtatgg taatgggctt tatcggcctg
    10381 ctggtgaaca tcccggtgaa ctatatcttt atttatggtc atttcggtat gcctgagctc
    10441 ggtggcgttg gttgtggcgt ggctactgcg gcggtgtatt gggtcatgtt ccttgccatg
    10501 gtttcttaca ttaaacgcgc ccgctccatg cgcgatattc gtaacgaaaa aggcaccgct
    10561 aagcccgagc ctgcggttat gaaacgactg attcaactcg gtttgccgat tgcgctggca
    10621 ctgttctttg aagtgacact gtttgccgtc gtggctctgt tagtgtctcc gctcggtatt
    10681 gttgatgtcg caggacacca gattgccctg aactttagtt cactgatgtt tgtgcttcca
    10741 atgtcgctgg cggcagcggt aactatccgc gtaggttatc gtctgggtca gggctcaacg
    10801 ctggatgcgc aaaccgctgc gcggaccggg cttatggtgg gtgtctgtat ggcaaccctg
    10861 acggccattt tcacggtttc actgcgggag caaatcgccc tgttgtacaa cgacaatccc
    10921 gaggttgtaa cgctggctgc gcatttgatg ttgctggcgg cggtatatca gatttctgac
    10981 tcaatccagg tgattggcag tgggattttg cgtggttata aagatacgcg ttccattttc
    11041 tatatcacct ttacagctta ctgggtgctg ggcttgccaa gcggctatat tctggcactg
    11101 accgatctgg tcgttgaacc tatggggcca gcaggcttct ggataggctt tattatcggc
    11161 ctgacatcgg cagccattat gatgatgctg cgtatgcggt tcctgcaacg tatgccgtca
    11221 gccatcattc tgcaacgagc gtcccgctaa taaagacaag gcgcaacctt cacgggttgc
    11281 gcctgtgttt ttacgcaggc tggagcgttg cgccaatccc gtcttcgtct ggctgtaatt
    11341 tcagagcgtt acacagagtt tgccgactgg catctgttat ctcaacgcgt atatccccgt
    11401 taacttcccc tttcacattg atgaagtcag gtgaaatttc tttcgcactg gtttgcaggc
    11461 aaatcgctcc ctctccgcgc aggcgtttca ctttcagttg cacatgcgtt gccgcaccag
    11521 tgatcagcca cagttttccg ccattcagat tgatgttacc aacaatgaaa gtgacgctgt
    11581 caccttttac cgtactgccg tcgagaatca acacaccgcc ctgctgtaca gtaatcgttg
    11641 attgttgcag ctctgcgcca taaccaataa tcattgaagc gccgtccagt aatgcaacac
    11701 tcccggtcag ttcaaccggt gcgcgatctt cctctacgac aaaattttcg tccgcgctgg
    11761 tgggtgacaa caggatcact gttccacctt gtattaccgc attttccagc gtaccggcat
    11821 gcacttcagc ccgccccccc acattcaccg acaggttttg cgtcttaccg gaactgtaga
    11881 gctgaaggcc atccgtcccc agacgataaa tggatccatt ctcaacaata gtgtcagagt
    11941 cttcaccacc gagattattc atggtgccac cagcaagcaa cgtatcactg gctttcccgg
    12001 ccagcataaa aacgccgccc tgattaatct cacaattgct ggcttctcca cttggataca
    12061 ccaacaattc accaccttca tctaccacga ccgctgaggt tgtcccatta accaacaggc
    12121 cgccctcttg atcgagaatg attttttccg cgcgatgtcc ttccagtaca cgcaggttac
    12181 cgccattttc cagcaacaaa ccgcaggcat aaccctgatc aacgctgaat tcaccttcgg
    12241 gatggcggcc attaaccgta gcgagcgtag agagactaat tgcgcctccc tcggactgaa
    12301 caatatctgt ggcaatggca ccgtcgctga ccgatagcgt gccattgtta ccgtactgtg
    12361 tgcatttggc gatcccaccg gttgccactt tcaaccagcc agcccccgtg accacggtat
    12421 tttcggctct tccgccagct tcaacaatca actcgccttc agtaagttgc gtcgcgccaa
    12481 ggacctcgcc ggtcttaaca atttgcacat tcccgtcgct catcaagttt ttcgcatcag
    12541 atcccatgat ttattccttt gctgcatctg tgtgccttta ttgctaccta agtgtaaagg
    12601 ctacggagga tttatccacg acagatttga gatggtggca aacaactctg tttaaactct
    12661 gatacacgaa ttattgggtt gtatcagatg taaatgcgat cctgaataaa aatcgccctt
    12721 gcaaatcaac aaaatatagc caatttgctt aaaagctctg caaacggtcg aaatagcgca
    12781 gaaaattacg ttttgcctct tgccaccttt ccactctgcc gctaatattc gtccccgttg
    12841 tcacctacaa cgttgcgttc atagctcagt tggttagagc accaccttga catggtgggg
    12901 gtcgttggtt cgagtccaat tgaacgcacc atcctgcgtc cgtagctcag ttggttagag
    12961 caccaccttg acatggtggg ggtcggtggt tcgagtccac tcggacgcac cagattttct
    13021 taatctggtc ttttcctttt tccctctgtt tcttctctgt atccaatacg ttaaaagatt
    13081 tacactgtct tcgtatgcgt tatcagaagg agaatcgcta tggcaacttt gttacaactt
    13141 cattttgctt ttaatggccc atttggcgac gcaatggctg agcagcttaa accacttgct
    13201 gagtcgatta atcaggaacc tggttttctg tggaaggtat ggacagaaag tgaaaagaac
    13261 cacgaagccg gtgggatcta ccttttcact gatgaaaaaa gcgcccttgc ctatctggaa
    13321 aaacatactg cccgactgaa aaatctcggc gttgaggaag ttgtcgccaa agttttcgat
    13381 gtcaatgagc cacttagtca aatcaatcag gcaaaactcg cctgacagaa tttaatcaag
    13441 ggcggttagc gcccttttca tccctgtctg aaatttctca aattctaaaa atctcaacca
    13501 aacttatctg ataacactaa attcgaaaga atgcgtacag gtaagtaaca atgaaaaaaa
    13561 ttgctattgt gggtgccggg cctacgggga tctacacctt attctcgctt ctacagcaac
    13621 aaactccact ttctatttct atcttcgagc aggctgacga ggccggtgtc gggatgccat
    13681 acagtgatga ggaaaactca aaaatgatgc tggcaaatat tgccagtatt gaaataccgc
    13741 cgattaattg tacgtatctc gaatggctac aaaagcaaga agccagccat ctccagcgtt
    13801 atggcgttaa aaaagaaacc ttgcacgatc gtcagttttt accgcgaatt ctgctgggcg
    13861 aatatttccg cgatcaattt ttacgattag tagaccaggc acgacagcaa aaatttgcag
    13921 tggctgttta tgaatcatgc caggttaccg atctgcaaat tacaaatgct ggcgtcatgc
    13981 tcgctacaaa tcaggattta cccagcgaga cgtttgattt agcggtgatt gccacgggtc
    14041 acgtctggcc tgatgaagaa gaagcaacca gaacgtattt tcccagcccg tggtcaggcc
    14101 tgatggaagc aaaggtcgat gcgtgtaacg tgggtattat gggaacatcc ttgagcggac
    14161 tggatgcggc aatggcagtg gctattcagc atggttcgtt cattgaagat gataaacaac
    14221 acgtcgtttt tcaccgcgat aacgcaagtg aaaagctaaa tattacgttg atgtcgcgca
    14281 cgggtatttt acccgaagcc gatttctatt gccctattcc ctacgagccc ttacacatcg
    14341 tcaccgatca ggcattaaat gctgagattc aaaaaggtga agagggcctt ttggatcggg
    14401 tatttagatt gatagtagag gaaatcaagt ttgctgatcc agactggagc caacgcatag
    14461 ccttagagag cctgaatgtc gattcctttg ctcaagcctg gtttgccgag cgcaaacaac
    14521 gcgacccatt tgactgggca gaaaaaaatc tccaggaagt cgaacgcaat aaacgagaaa
    14581 aacatactgt tccctggcgt tatgtcattc tgcgcctgca tgaagccgta caggaaattg
    14641 ttcctcatct gaatgaacac gaccataaac ggttcagtaa aggccttgcc agggttttca
    14701 tcgataatta tgcggcaatc ccttcagagt ctattcgtcg actactggcc ttacgtgaag
    14761 cggggatcat tcatattctc gccctcggtg aagactacga aatggaaatt aatgagtcgc
    14821 gcaccgtcct gaaaacggaa gacaacagct actcgtttga cgtttttatt gatgcccgcg
    14881 gacagcgtcc gcttaaagtg aaagatattc ctttccctgg gctacgcgag caattacaga
    14941 aaacagggga tgaaatccct gatgttggcg aagattatac gttacagcaa cccgaagata
    15001 ttcgtggacg cgtagcgttc ggcgcgttgc cctggttgat gcacgaccag cctttcgttc
    15061 agggacttac ggcatgtgca gaaattggtg aggcgatggc tcgggcggtc gtaaagcctg
    15121 catcccgtgc tcgtcggcgt ctttcgtttg attaaaggta aagctatcag cactcaacaa
    15181 actgatgctg ttcattaaga cgatagcgga ctccggttcg aatattgttt tcaccttcaa
    15241 tggcgacggc gaagcgaact ctttcgccgt catgatatgc cagtgcggcg cttcccccta
    15301 gcccgagaat aattgagtcg accacgcccg tgctcactac cgttgaattt tcaccgctgg
    15361 caatgatgtg tacattgtcg ccgctgttgg caattctggc gtttgcgcca aagctggcga
    15421 tttgcaccag gtcaccatta ctggcgacat gacaccgttc acccaatgta caaacgcgta
    15481 ctcgcattcc ggtattcgca atccgactac tatctccagc gctgcttatc cgcgaagaat
    15541 taccggttac agcaacccgt gcgcgttcac cggagctgcc aatgtgactg ttaaagccga
    15601 cacttccaat ccgcacgcta taacccatac tggcaatttg cgcggcatac ccagcggttg
    15661 cgattcgggc gttatcctca caacagccga tgcgagcatg atcgtcgctg cgggcaaact
    15721 gactgcaaaa tggtatctct ccggggtctg tggccagttt ttgcatagag gaaacttcct
    15781 gatgcgcgaa gttttcatcc gccagccatt ttttccacgc atactcaacc agactctccg
    15841 cccagtccgt atatccttcc tcaatcagtg catgacgaat atcggcataa ctaccgccgc
    15901 gtgggaaatg gcgcaggaac cagcgataca tcaccgctcc gatacgccat tctcgcaggt
    15961 ctgctcgtgt aatgatcatt cgttagtgcc ggtgatagcc atcgaccatg cttttaaagg
    16021 tttcgtgtgg cgtacgcccc gtggtgcaaa gataaagatg accgaagata aaaaagaggc
    16081 ttataaatgc cagagcaaaa tgcgcctgca ataaccagta tcttacgcca ggaaacacat
    16141 ctcccacggc ttgcggatag agacacaaca gccccgtcaa tagtaacaac ggcagcaatc
    16201 catacatgac accaacatag gcgacctgct gtaagggatt aaatttagac tgtgttgttg
    16261 ccgggaaagg atgttcttcc ccctgcataa tgccaaacaa ataaaatcgc gtttgttttg
    16321 ccgctcgttc cagccacccc tgacgacgaa tgcgatagtg gtgaccatta tccccaacgg
    16381 cattgatcag cacaaagccg agccagcatg ccagtaacaa aaatccgcaa acttcatgca
    16441 ccgcaaccag acttttaacc gcagttgcgc ccaccatcgc aaagtgattt atcagcccac
    16501 ttgccagcaa caatacaaag agtaacgcat tcgaccagtg ccataatctg accgctttgc
    16561 tgtaaagata aaccttctca ccatgatcgg tggctgattt cttcacgcca cgagcacgaa
    16621 gcaaggcgtg cagccccagc accaaccaca tcccaaccag caatactccg gcaatgatca
    16681 accacaccgg ccaaaattcg ggagtgaact gtggcacata gttcgctaac tggctctgaa
    16741 actgttcagc atgttgcgac gggttcatac attttccttt ttaatcaaat gttgaccgaa
    16801 ccggcgatac agatgcggtt ttccggcacc aggtagctga tattgatagt atttattatc
    16861 ctgcaaccac gcctgaattt ctgggctatc ttcgcgacca aagatcaatg cgtgttccgg
    16921 acaggcgctg acgcaaatgg gcggaaagcc ttttgccagt cgggactcag cgcagaaatc
    16981 acatttatct gccactttag tgacagggtt aaggtagcga acctggtacg gacaagcgcc
    17041 aatgcaataa ctgcagccga tgcactgcga tttttctacc cgcacgatcc cctgctcatc
    17101 acgccacgat gcccccgtcg ggcatacgtc gatgcacgga gcatcttcgc agtgctgaca
    17161 tgactggcga aaaaagtgat actgcgtttc gttgtcgtta tcggtgactg gaatgtgtgc
    17221 tatcgataaa cggcttccct gagcggggac gtggttagtt ttacggcatg cgcgggcgca
    17281 aatattacag ccattgcacc gtgactcgtc atgaatcatg gcataccgaa cttccttttc
    17341 ttgcctcgtg ttcgctaaca gagaagacgc agaaccagta aaaaagatta ctgttcccat
    17401 ccccagaaca aatttgcgtc gagtaaagga catctgatta tcccctaatg cagtggctgc
    17461 aactgtccac cctatccatg acatctccct gaaatttcag gggttctgaa actgctctac
    17521 ctcgaaccat tcgcataagc cataagcctt gccataggtc ataaatcgct cgctaaacaa
    17581 gtacaacacc gccgtcgatg actccacctg ccgtatatcg gcatattcag gtgcaacatc
    17641 catcgcggcg atcgcggctt caatctgcgc ttcctggaag taatatggcg cttgcatcag
    17701 catggcgact ttgtaaggac gaggatacgt ctggcactca aaacgaacgg cgtgtgcaat
    17761 ggcgcgacaa atgtcctgct ccacaacttg caaagacatc gccgcgtagt tttcactcat
    17821 ggcctgcgtt gagtaatagt aatcatcctg acttcccgtc atacaagcga tatccgcgta
    17881 gtctccgttc tgcttaatgc tttccagcaa aactgacagt tcctcttctg caaccgaata
    17941 gggtggcgag agaaacacgg tgcgcgccac cagctgccca gccgcagaat gctggcgaat
    18001 gaactcagcc aggatttcag caggttctgg ttcggttacc gtatcaacgg ggtgacgcat
    18061 cccctgcaac cattgccgct tcgcctcgtc aacttcgctg actttcgcta acggcaattc
    18121 atcctgatgg ttcatcttac ccattcctta cgcaggcagt agattgtgtg ctgccagatc
    18181 ggcggcaata tcttccagcc ccagacgctg caatgtttcg cgggttggac aaccaagctg
    18241 tgggtcccag cccatttctt tgtagaacat cgtgagcgaa gcatgcatat cgtcacgatc
    18301 cattttgtca gtaccttcag taaagaccgg gatctgcgga tccttgtcga aaacccagga
    18361 acagataaga tcgtgttcat tacgcatatc tttggtttgc atcggtttta ccgtgtaggc
    18421 acgatgcaac gtaaagatac gctctgcagc taaatctaat ttttcctgag tcatctcttc
    18481 gccggtgatc gctttgaaga atttggctta aagcgccaga tcgccccggt aattacggct
    18541 tttcagcggc gaaacggtca ttggccagac ccagttgcac agagtgacgg cgttatgcaa
    18601 acagacccgc aacagcgacc actttgcata tttaattttt gcgtcattga ttggcgtgta
    18661 gtttttggtt tcatcgtaag catcttcaga accaaaaagt tctttcgcca cttcacgttg
    18721 cagtttcaat ggcaagccgg aaccaataaa gttgatatgg gtatgcgtca tgcagtcacg
    18781 gttgaacata cagttaacaa tggaaccgac ctgcgctgac gcttcattgg catggtgaac
    18841 cggatagcca aacggcgacc agagtttatt tttcgcgtag ccccagtact ctttacccaa
    18901 attccagcgt tctgcgatgg catatgaacc atcagccagg tgactcagct cacccacacg
    18961 atgcgccaga cggtagtaaa aatctttaat gaagttaacg tcacccgctt ccagttgatc
    19021 ccagcgaatt tctgcatact cttcagctgg cagaacacgc ttgaacacac ctttgctgta
    19081 acaataagta aagtcgcgat gcaactgccc gtagttacac cataggccgt agtcatcgaa
    19141 caaattcaga ccaaccaggt taccaatcac acgaccatcg tctttatctt caaaatcttt
    19201 cggcccgttc gggaagatgg tggtatggac aaagtttgct acacaagtgt taccacctgt
    19261 gctgggcacg ccaaactctt tcacccgagg aatattcatt tgggtcatac aacggatcgg
    19321 gcaagagtgg cagccgctca tttttactgt gtatttctct gccgccggtc ctaagtcaaa
    19381 aacggattta taggtacgaa agccgaccgt attctgattg cctggcggaa tttcacccgt
    19441 ttcaatcgga ccaccttcag ccgcgcccca aaacagccct ttacgtgctg tccagcgtga
    19501 cttgggatct gaatactctg cccacgattg tggcgtactt ggcacgacat ggttgttatt
    19561 cgcaccaata agttcagtca tcatgtaatc attgagacgc ttcatctcct gacgatcggc
    19621 aatgttgacc cctttcgtcc cttcaaccgc aatcgctttc aggtttttcg aacccattat
    19681 tgcgccagtt cccgcaccgc cgctgtggtt acggctattc aacatgccag agagaggaat
    19741 aaggttttcc ccagcctgac caatagccgc cacacaggtt tccgggctgg tcaatcgaca
    19801 aatttcttcc gtcgttgcgc gcgtcccttt tccccataag aaatcggctt tttccaggct
    19861 aactttgtca tctttaatct tcagccatac cggtgatttc gccttccctt cgataataat
    19921 gacgtcgtat ccagcgaatt tcatttgcgc tgcaaaaaag ccacccatat gggcatcgcg
    19981 actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga actacagggg
    20041 gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc atcgaaaggt
    20101 ttcgtgccta gcggtacttc gtcatacata attttgtagc cgaagcccat gccaccgaca
    20161 aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag attgactctt
    20221 aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt cacacagtaa
    20281 tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg cattcaccgc
    20341 ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc atccacgggc
    20401 atgccgtagt acaggcgcta cagccaatac aacgtttatg atcgacggta atacagcctt
    20461 ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc ggctctttgc
    20521 attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg ccagagccaa
    20581 ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag aatgtcccta
    20641 ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc gtgcatcgcg
    20701 ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg ccgctatcaa
    20761 tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt agaccagcaa
    20821 ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct aatagtggac
    20881 gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga caattcagca
    20941 gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac cagatatttt
    21001 ccctaacccg atagtgtatg agaaaagatt taacgccgat aaactaatcc tttatggtta
    21061 ggagaaaaaa taatgtgatt attctgagcc cttaacattg atcgttatca attaaaatta
    21121 caaagcaaga aattataagc gaactgatat ttattatcat ttgaaataaa tttaacttaa
    21181 gcgacaatca tttgtgacgt tcattgcaga aaataaaaat aacattatcg ctataaatta
    21241 aaatacatac cgaaattcgt ataatttcag cagttgttat ttagaatgat taactggacc
    21301 gcaactgaag caagaatatc aggatgaagc ttaccgcctc atcctgaatg gtcattttac
    21361 cagtgcgtta atgacggttt ccatcgcctg cttttccagc tcactgcgct gaatgcgttt
    21421 attggctaat gctgtacgta atacgcctgc gataacaata tgctttggtt cctgccccag
    21481 atcgcgcatt tccagcacaa ctttaccgac aactctgcac atttctcggt acagttcatc
    21541 ctcttttgtc cgatttccca ttctttatac ctatttatca tgccaact
//
LOCUS       DAJVRL010000065        21502 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVRL010000065 DAJVRL010000000
VERSION     DAJVRL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.065"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OQN02_002514"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OQN02_002514"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1643261.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OQN02_002515"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OQN02_002515"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1643262.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OQN02_002516"
     CDS             complement(3121..3420)
                     /locus_tag="OQN02_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1643263.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OQN02_002517"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OQN02_002517"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1643264.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OQN02_002518"
     CDS             complement(4632..5069)
                     /locus_tag="OQN02_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCS1643265.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OQN02_002519"
     CDS             complement(5115..5456)
                     /locus_tag="OQN02_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643266.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OQN02_002520"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OQN02_002520"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1643267.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OQN02_002521"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OQN02_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OQN02_002522"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OQN02_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1643268.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OQN02_002523"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OQN02_002523"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCS1643269.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OQN02_002524"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OQN02_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCS1643270.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13864
                     /gene="xylR"
                     /locus_tag="OQN02_002525"
                     /pseudo
     CDS             12687..13864
                     /gene="xylR"
                     /locus_tag="OQN02_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13972..14796)
                     /locus_tag="OQN02_002526"
     CDS             complement(13972..14796)
                     /locus_tag="OQN02_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCS1643271.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="OQN02_002527"
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="OQN02_002527"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1643272.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="OQN02_002528"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="OQN02_002528"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCS1643273.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="OQN02_002529"
     CDS             complement(18728..19201)
                     /locus_tag="OQN02_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1643274.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OQN02_002530"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OQN02_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCS1643275.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="OQN02_002531"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="OQN02_002531"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCS1643276.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21502
                     /locus_tag="OQN02_002532"
     CDS             21362..>21502
                     /locus_tag="OQN02_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1643277.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5398 a         5487 c         5282 g         5335 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaactggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gtcttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgggcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcgcccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatata catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acgcctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctgg ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag ggcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagcct gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgttgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgctcag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagttccgc atattgaaat tttaagccaa aaaagcgatc aaaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cg
//
LOCUS       DAJVRL010000066        21387 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVRL010000066 DAJVRL010000000
VERSION     DAJVRL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.066"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..134)
                     /locus_tag="OQN02_002533"
     CDS             complement(<1..134)
                     /locus_tag="OQN02_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1643278.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="OQN02_002534"
     CDS             complement(236..1216)
                     /locus_tag="OQN02_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1643279.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="OQN02_002535"
     CDS             complement(1224..1934)
                     /locus_tag="OQN02_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCS1643280.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OQN02_002536"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OQN02_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1643281.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="OQN02_002537"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="OQN02_002537"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1643282.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="OQN02_002538"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="OQN02_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1643283.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="OQN02_002539"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="OQN02_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1643284.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="OQN02_002540"
     CDS             8511..8621
                     /locus_tag="OQN02_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1643285.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OQN02_002541"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OQN02_002541"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1643286.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OQN02_002542"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OQN02_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1643287.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="OQN02_002543"
     CDS             11503..12135
                     /locus_tag="OQN02_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1643288.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OQN02_002544"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OQN02_002544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1643289.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="OQN02_002545"
     CDS             15667..16599
                     /locus_tag="OQN02_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1643290.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="OQN02_002546"
     CDS             complement(16640..18070)
                     /locus_tag="OQN02_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1643291.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OQN02_002547"
     CDS             complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OQN02_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1643292.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OQN02_002548"
     CDS             complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OQN02_002548"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1643293.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19931..20200)
                     /locus_tag="OQN02_002549"
     CDS             complement(19931..20200)
                     /locus_tag="OQN02_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1643294.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20270..20854
                     /gene="mobA"
                     /locus_tag="OQN02_002550"
     CDS             20270..20854
                     /gene="mobA"
                     /locus_tag="OQN02_002550"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1643295.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20836..21363
                     /gene="mobB"
                     /locus_tag="OQN02_002551"
     CDS             20836..21363
                     /gene="mobB"
                     /locus_tag="OQN02_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1643296.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5233 a         5365 c         5143 g         5646 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa
    18181 aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac
    18241 agatatttca ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata
    18301 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc
    18361 acatcagctg cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac
    18421 gccgcgtcgt actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat
    18481 gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct
    18541 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca
    18601 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca
    18661 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa
    18721 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca
    18781 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa
    18841 atctttttca tgatctactc tctccgatta atacataggt gttaattgca aagggggttc
    18901 ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc
    18961 ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc
    19021 aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc
    19081 gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc
    19141 tttatcgcca ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc
    19201 cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg
    19261 tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc
    19321 tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag
    19381 cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg
    19441 tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat
    19501 attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg
    19561 atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt
    19621 taccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg
    19681 gcgataaaat ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt
    19741 ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc
    19801 gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc
    19861 atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc
    19921 ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt
    19981 cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca
    20041 ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt
    20101 tcgccagttt ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct
    20161 ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg
    20221 agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat
    20281 gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga
    20341 taaaggattg ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa
    20401 gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg
    20461 cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct
    20521 ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta
    20581 cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg
    20641 ggtccatgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc
    20701 tttattactg gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc
    20761 tggcggtcat gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc
    20821 agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg
    20881 agtggtaccg gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg
    20941 atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa
    21001 gatagctatg agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga
    21061 tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt
    21121 cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc
    21181 gcaaagattg tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac
    21241 aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat
    21301 attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga
    21361 taagcaagag taggctggat cagacgc
//
LOCUS       DAJVRL010000067        21274 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVRL010000067 DAJVRL010000000
VERSION     DAJVRL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.067"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..444)
                     /locus_tag="OQN02_002552"
     CDS             complement(<1..444)
                     /locus_tag="OQN02_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1643297.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OQN02_002553"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OQN02_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1643298.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OQN02_002554"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OQN02_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1643299.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OQN02_002555"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OQN02_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1643300.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="OQN02_002556"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="OQN02_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1643301.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="OQN02_002557"
     CDS             complement(5214..6812)
                     /locus_tag="OQN02_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1643302.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OQN02_002558"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OQN02_002558"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1643303.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="OQN02_002559"
     CDS             complement(8491..9069)
                     /locus_tag="OQN02_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1643304.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OQN02_002560"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OQN02_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1643305.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="OQN02_002561"
     CDS             10508..10687
                     /locus_tag="OQN02_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1643306.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="OQN02_002562"
     CDS             complement(10807..11166)
                     /locus_tag="OQN02_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1643307.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="OQN02_002563"
     CDS             complement(11528..11872)
                     /locus_tag="OQN02_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1643308.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="OQN02_002564"
     CDS             12004..13914
                     /locus_tag="OQN02_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1643309.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="OQN02_002565"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="OQN02_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1643310.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="OQN02_002566"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="OQN02_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1643311.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15493..17178
                     /gene="fadD"
                     /locus_tag="OQN02_002567"
     CDS             15493..17178
                     /gene="fadD"
                     /locus_tag="OQN02_002567"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1643312.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17260..18375
                     /gene="rnd"
                     /locus_tag="OQN02_002568"
     CDS             17260..18375
                     /gene="rnd"
                     /locus_tag="OQN02_002568"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1643313.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="OQN02_002569"
     CDS             complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="OQN02_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1643314.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="OQN02_002570"
                     /pseudo
     CDS             complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="OQN02_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20409..20975)
                     /locus_tag="OQN02_002571"
     CDS             complement(20409..20975)
                     /locus_tag="OQN02_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1643315.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21187..>21274
                     /locus_tag="OQN02_002572"
     CDS             21187..>21274
                     /locus_tag="OQN02_002572"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1643316.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5267 a         5273 c         5466 g         5268 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta caacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcacc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat tgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatccttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcaggggttc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgggtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgacg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtcc cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaaaaag aaacagcggc tggtccgctg tttctgcatt cttacggtaa agataaaaat
    15361 aaatagtgac gcgcttcgca accttttcgt tgggtaatta tcaagctggt atgatgagtt
    15421 aatattatgt taacagcgtg tatatcattt ggggttgcga tgacgacgaa cacgcatttt
    15481 agaggtgaag aattgaagaa ggtttggctt aaccgttatc ccgcggacgt tccgacggag
    15541 atcaaccctg accgttatca atctctggta gatatgtttg agcagtcggt cgcgcgctac
    15601 gccgatcagc ctgcgtttgt gaatatgggg gaggtgatga ccttccgcaa gctggaagaa
    15661 cgcagtcgcg cgtttgccgc ttatttgcaa caagggttgg ggctgaagaa aggcgatcgc
    15721 gttgcgttga tgatgcctaa cttattgcaa tatccggtgg cgctatttgg cattttgcgt
    15781 gccgggataa tcgtcgtaaa cgttaacccg ttgtataccc cgcgtgagct tgagcatcag
    15841 cttaacgata gcggcgcatc ggcgattgtt atcgtgtcta actttgccca cgctctggaa
    15901 aaagtggttg ataaaaccgc cgttcagcac gtaattctga cccgtatggg cgatcagcta
    15961 tctacggcaa aaggcacggt agtcaatttc gttgttaaat acatcaagcg cttggtgccg
    16021 aaataccatc tgccagatgc catttcattt cgtagcgcac tgcataacgg ctaccggatg
    16081 cagtacgtca aacccgaact ggtgccggaa gatttagcct tcctgcaata caccggcggc
    16141 accacaggtg tggcgaaagg cgcgatgctg actcaccgca atatgctggc gaacctggaa
    16201 caggtcaacg ccacctatgg gccgttgctg catccaggta aagaactggt agtaacggca
    16261 ctgccgctgt atcacatttt tgccctgacc attaactgcc tgctgtttat cgaactgggt
    16321 gggcagaacc tgcttatcac taacccgcgt gatattccag ggttggtaaa agagttagcg
    16381 aaatatccgt ttaccgctat cactggcgtt aacaccttgt tcaatgcgtt gctgaacaat
    16441 aaagagttcc agcagctgga tttctccagt ctgcatcttt ccgcaggcgg tgggatgcct
    16501 gttcagcaag tggtggcaga acgctgggtg aaactgaccg gacagtatct gctggaaggt
    16561 tatggcctga ccgagtgcgc gccgctggtg agcgttaacc cgtatgatat tgattatcat
    16621 agcggtagca tcggtttgcc ggtgccatcg acggaagcca aactggtgga tgacgacgac
    16681 aacgaagtac caccgggtca accgggtgag ctttgtgtca aaggaccgca ggtgatgctg
    16741 ggttactggc agcgtccgga tgctacagat gagatcatca aaaatggctg gttacacacc
    16801 ggcgacatcg cggtgatgga tgaagaagga ttcctgcgca ttgtcgatcg taaaaaagac
    16861 atgattttgg tttccggttt taacgtctat cccaacgaga ttgaagatgt cgtcatgcag
    16921 catcctggcg tacaggaagt cgcggctgtt ggcgtacctt ccggctccag tggtgaagcg
    16981 gtgaaaatct tcgtagtgaa aaaagatcca tcgcttaccg aagagtcact ggtgactttt
    17041 tgccgccgtc agctcacggg atacaaagta ccgaagctgg tggagtttcg tgatgagtta
    17101 ccgaaatcta acgtcggaaa aattttgcga cgagaattac gtgacgaagc gcgcggcaaa
    17161 gtggacaata aagcctgagc gttaagtcag tcgtcagacg ccggttaatc cggcgttttt
    17221 tttgacgccc actaaagaga aaacaatttg aattaccaaa tgattaccac ggacgatgcg
    17281 ctggcttctt tgtgtgaagc cgtccgtgtc tttccggcga tagccctgga tactgaattt
    17341 gttcgtacgc gcacttatta cccgcagctg gggttgattc aacttttcga tggcgagcat
    17401 ctggcgctaa ttgatcctct cgggatcacc gactggtcac cgctgaaagc gatcctgcgc
    17461 gatccgtcca tcacaaaatt tctccatgca ggcagtgaag atctggaagt gttcctcaat
    17521 gtctttggcg aattaccaca acccttgatt gacacgcaaa tccttgctgc cttctgcgga
    17581 cgcccgatgt catggggttt cgcttccatg gtggaagagt attccggcgt tacgctggac
    17641 aagagtgaat cgcgcaccga ctggctggcc agaccgctga ccgaacgtca gtgtgaatac
    17701 gcagcggcgg atgtctggta tctgttaccg atcaccgcca agcttatggt agaaacggag
    17761 gcctccggct ggctacctgc ggcgctggat gaatgccgcc tgatgcaaat gcgtcgtcag
    17821 gaagtcgttg cgccggaaga tgcctggcgt gatatcacca atgcctggca gttacgcaca
    17881 cgccaactgg cctgtctgca actgttagcc gactggcgac tgcgcaaggc gcgagagcgt
    17941 gatctggcgg tgaactttgt cgtgcgtgaa gagcatttgt ggtcggtcgc gcgttatatg
    18001 ccgggaagtt taggcgaact ggacagcctg ggtttatccg gtagcgaaat ccgctttcac
    18061 ggtaaaacgc tgctggcgct ggtggaaaaa gcgcagacat tgccggaaga tgccttaccg
    18121 cagccgatgc ttaacctgat ggacatgcct ggttatcgta aagcgtttaa agcgattaag
    18181 tcgctgatta ctgacgtgag cgaaacgcat aagatcagcg ccgaattgct ggcatcgcgt
    18241 cggcaaatca accaactgct gaactggcac tggaaactga aaccgcagaa caatttgccg
    18301 gagctgattt ccggctggcg tggtgagctg atggcggaag cattacacaa tttattgcag
    18361 gaatatccgc agtaaaatct tccgaagccg gactgggcgc gctcagcgcc acatccggct
    18421 ttggcaaact acaaatccaa caccaggcgt ttacccttcg cacgcgaaca acagatcaac
    18481 atactttgct ggctggcacg ctcttcatcg ctaaaatact gatcccgatg gtcagcttca
    18541 ccttccagta ttgccgtttc gcaggttccg catacccctt cacgacataa acattccact
    18601 ttcgcggctt tattattttc aataacctgc aaaatagtca tctcttccgg caccacaaac
    18661 tcttttccgg aacgggcaag caccagggta aatgcatcgc cggttttgtc ttcgatagca
    18721 aattgctcaa agtgcagtgt atcggcggcg atgtccagac gcgcagcttc acttcttacc
    18781 gcttcaatta gcgcctcggg gccacaggta taaacgtgtg tgccaggttc gatatccgcc
    18841 aataatcgcg ctaattccag tcgtgttccg gttgatgaat gatgcaaatg gactttctca
    18901 gcctgcggat gctggactag ctcagcacga aatgcgcaac tttctggatt tcgcgagcag
    18961 taatgtagct gccagtcgat gtcgctgtgt tgcaactctg ccatgtgcga caggaaaggg
    19021 gggataccaa taccgcccgc gataaacaga tgctttctgg ctgagggaat tagcgcaaac
    19081 aggttattag gcgttgagat cgttaaccga tcgcccactt ttacctgctg atgcaaaaag
    19141 cgggaaccgc cgcgcgagtt ttcttccagt cgaacggcaa tctgataact agaggtgtta
    19201 tgcggtgaac tcagtagtga atacgcattg ctgtactggt tatcaccatc gctcatctgc
    19261 actaggatgt gacttcctcc ggtgaacgca ggcagcggtt tgccatcggt tgccaccagc
    19321 gtgaagcgtt tcacctgttc ggtaaggggt tcaacctggc tcacctgtac ttcaaacatt
    19381 tgatagtctg acattgctgt actccatcaa aaatcagccc agcggcggaa aataccgccg
    19441 ccgatgtcat tagtccttaa acacctgcgc cagcagatta tggaaatggg cgataccatg
    19501 ttcggaaatg ccactaccgc tactgtcggc catgatgcgc ccctgaccac gatagccacg
    19561 cgatttcagc cctttctgta cgctttcaac cagacgtaaa tcttccggat ggaacacatc
    19621 gcgataccac tcaatcagcg atttttgctc gtcggttaac tcttcattgg tgaagtaaat
    19681 atcgtagttt tgcagggtag tttcagaatc caccgggaat tcataaatga ccgtcatcat
    19741 ccctttgatc ggggtgacgt tcagcatcgt gcacggccac agccagaaac cgtggaatgc
    19801 cgcatccgta ccctcttcaa atttaaacga ctgttcggac ggtttggcga aaccgtattg
    19861 cagcgtccag ttaccgtgca tggtgtgcca gtaacgatca acctgtacgg agtcggagaa
    19921 acctagatgc gccggaccac agtgatagca ctcgagatag ttatcgacaa tgttcttcca
    19981 gttggcaggc gtgcgggtgg taaagcgggc cgctagtttc agattgtgga cttccgggca
    20041 ggcttccagc actttcgcgc ccagaccggt gtattgattc acttgaagta cgaaaaaaaa
    20101 ccgggaggac attggattat tcgggttctg atgggattag atttggtggg gcttgcaagc
    20161 ctgtagcgca aattttagtc gttaatcaat gaaacgcgaa agatagtaaa aaattgcttt
    20221 tgtttcattg aaaatacgaa aaacaaaaac actgcaaatc atttcaataa cagcttcaaa
    20281 aaacgttcaa aaccgataac aaccaggctg taaccaaatg actcatatca caaatcagct
    20341 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggtt
    20401 catggttgtt atacggcttt ttttacatct tgtggttctt gtgaaggagc tacaagacct
    20461 tcctgttcat aaataccata ctttgacaaa atctttttac gagtatctgt tggtaatgat
    20521 tcaagcattt tggctgtttg taaggtttgc tgcgaaatac cttgcaaatc agcctggatg
    20581 gaatcatcgg aagaaaaaat ctcaggcata agagcttcaa atcctttctt tctgaacaac
    20641 tcaatcgcag aatgcagttc cttgtcctct aacaattcga caattgcgat cagtttagtt
    20701 ttcaatgtgc tgcggtcagg ttgagaattt ttattctctt gattattttt atgctcaacc
    20761 attggcatgc tctgactttg gataaaccac tctattggac gccctgttgc gtaggctatt
    20821 tgtgctaatc gatctaaaga cgggtatgtc ttgccagaca ggtagtttct gataacccca
    20881 tcagaaagtc cgcacttagc tgcaaacgcc cggcttgatt ctccattcat tgcctcttca
    20941 aggcgttcct tgaaacgcat tatttccggg ggcataaaaa tgcgctctgc atttttgctt
    21001 tctttttcac ctgtcatgcc caatctctct gttatataaa gaattttaca gaattctttg
    21061 atttgcactg gttaaaataa aaatgagcat agctcttgaa ttgagcattt ttctgcggta
    21121 tcgtttttcc cgatagcggt agccggtatc agttaccgaa aacgttaaga gagtaaggat
    21181 cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc agataaaggc tgctgttcgt
    21241 atgaagggga agacactatc tcaattatcc agag
//
LOCUS       DAJVRL010000068        21254 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVRL010000068 DAJVRL010000000
VERSION     DAJVRL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.068"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..791
                     /locus_tag="OQN02_002573"
     CDS             <1..791
                     /locus_tag="OQN02_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCS1643317.1"
                     /translation="AAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQI
                     AVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEKV
                     TDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTDH
                     YYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFSF
                     FFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OQN02_002574"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OQN02_002574"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1643318.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OQN02_002575"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OQN02_002575"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1643319.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OQN02_002576"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OQN02_002576"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1643320.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OQN02_002577"
     CDS             complement(4989..7223)
                     /locus_tag="OQN02_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1643321.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OQN02_002578"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OQN02_002578"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1643322.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OQN02_002579"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OQN02_002579"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1643323.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OQN02_002580"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OQN02_002580"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1643324.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OQN02_002581"
     CDS             complement(12978..14318)
                     /locus_tag="OQN02_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1643325.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OQN02_002582"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OQN02_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1643326.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFYNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OQN02_002583"
     CDS             complement(16106..16555)
                     /locus_tag="OQN02_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1643327.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OQN02_002584"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OQN02_002584"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1643328.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OQN02_002585"
     CDS             complement(17355..17684)
                     /locus_tag="OQN02_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1643329.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OQN02_002586"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OQN02_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1643330.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OQN02_002587"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OQN02_002587"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1643331.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OQN02_002588"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OQN02_002588"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1643332.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5669 c         5072 g         5287 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctggtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat gccgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgtagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccatg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatatcg
    16861 gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaatgc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccgc tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tccc
//
LOCUS       DAJVRL010000069        20836 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVRL010000069 DAJVRL010000000
VERSION     DAJVRL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.069"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OQN02_002589"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OQN02_002589"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1643333.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OQN02_002590"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OQN02_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1643334.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OQN02_002591"
     CDS             complement(2649..3200)
                     /locus_tag="OQN02_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1643335.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OQN02_002592"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OQN02_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1643336.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="OQN02_002593"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="OQN02_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS1643337.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OQN02_002594"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OQN02_002594"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1643338.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OQN02_002595"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OQN02_002595"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1643339.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OQN02_002596"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OQN02_002596"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1643340.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OQN02_002597"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OQN02_002597"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1643341.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKERVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OQN02_002598"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OQN02_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1643342.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="OQN02_002599"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="OQN02_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1643343.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="OQN02_002600"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="OQN02_002600"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1643344.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OQN02_002601"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OQN02_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1643345.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OQN02_002602"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OQN02_002602"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1643346.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OQN02_002603"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OQN02_002603"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1643347.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OQN02_002604"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OQN02_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1643348.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="OQN02_002605"
     CDS             complement(18174..18569)
                     /locus_tag="OQN02_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1643349.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OQN02_002606"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OQN02_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1643350.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="OQN02_002607"
     CDS             19485..20078
                     /locus_tag="OQN02_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1643351.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="OQN02_002608"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="OQN02_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1643352.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4962 a         5227 c         5520 g         5127 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtaat tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacccgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg ggcaaaatat cagatttgaa
    20821 ttattctcaa cacatt
//
LOCUS       DAJVRL010000070        20804 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVRL010000070 DAJVRL010000000
VERSION     DAJVRL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.070"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..558
                     /locus_tag="OQN02_002609"
     CDS             <1..558
                     /locus_tag="OQN02_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCS1643353.1"
                     /translation="SYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLS
                     PVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQN
                     ILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYN
                     SRRISLKLKGLTPIEYRNQTYMPRV"
     gene            660..812
                     /gene="hokA"
                     /locus_tag="OQN02_002610"
     CDS             660..812
                     /gene="hokA"
                     /locus_tag="OQN02_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1643354.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(999..1211)
                     /gene="cspA"
                     /locus_tag="OQN02_002611"
     CDS             complement(999..1211)
                     /gene="cspA"
                     /locus_tag="OQN02_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1643355.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1492..1782)
                     /locus_tag="OQN02_002612"
                     /pseudo
     CDS             complement(1492..1782)
                     /locus_tag="OQN02_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2216..2926
                     /locus_tag="OQN02_002613"
     CDS             2216..2926
                     /locus_tag="OQN02_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1643356.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2976..3950)
                     /gene="ghrB"
                     /locus_tag="OQN02_002614"
     CDS             complement(2976..3950)
                     /gene="ghrB"
                     /locus_tag="OQN02_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1643357.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4054..4704)
                     /locus_tag="OQN02_002615"
     CDS             complement(4054..4704)
                     /locus_tag="OQN02_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1643358.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4867..7200
                     /gene="bisC"
                     /locus_tag="OQN02_002616"
     CDS             4867..7200
                     /gene="bisC"
                     /locus_tag="OQN02_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1643359.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7169..7609)
                     /locus_tag="OQN02_002617"
     CDS             complement(7169..7609)
                     /locus_tag="OQN02_002617"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1643360.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7606..8169)
                     /gene="tag"
                     /locus_tag="OQN02_002618"
     CDS             complement(7606..8169)
                     /gene="tag"
                     /locus_tag="OQN02_002618"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1643361.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8327..9025
                     /locus_tag="OQN02_002619"
     CDS             8327..9025
                     /locus_tag="OQN02_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1643362.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9254..10462
                     /locus_tag="OQN02_002620"
     CDS             9254..10462
                     /locus_tag="OQN02_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1643363.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10786..12477
                     /gene="eptB"
                     /locus_tag="OQN02_002621"
     CDS             10786..12477
                     /gene="eptB"
                     /locus_tag="OQN02_002621"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1643364.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12569..12645
                     /locus_tag="OQN02_002622"
     tRNA            12569..12645
                     /locus_tag="OQN02_002622"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12603..12605,aa:Pro,seq:cgg)
     gene            13389..14996
                     /gene="dppA"
                     /locus_tag="OQN02_002623"
     CDS             13389..14996
                     /gene="dppA"
                     /locus_tag="OQN02_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS1643365.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15304..16323
                     /gene="dppB"
                     /locus_tag="OQN02_002624"
     CDS             15304..16323
                     /gene="dppB"
                     /locus_tag="OQN02_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1643366.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16333..17235
                     /gene="dppC"
                     /locus_tag="OQN02_002625"
     CDS             16333..17235
                     /gene="dppC"
                     /locus_tag="OQN02_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1643367.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17246..18229
                     /gene="dppD"
                     /locus_tag="OQN02_002626"
     CDS             17246..18229
                     /gene="dppD"
                     /locus_tag="OQN02_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1643368.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18226..19230
                     /gene="dppF"
                     /locus_tag="OQN02_002627"
     CDS             18226..19230
                     /gene="dppF"
                     /locus_tag="OQN02_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1643369.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19260..20531)
                     /locus_tag="OQN02_002628"
     CDS             complement(19260..20531)
                     /locus_tag="OQN02_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1643370.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5026 a         5114 c         5440 g         5224 t
ORIGIN      
        1 tcttacagag gagaggtagg gcaaaccgcc cctaatgttc tccaaagaga tttcaaggct
       61 acgcggccaa acgagaagtg ggttaccgat gttactgaat ttgcagtcaa tgggcgcaag
      121 ctgtatttgt ctccagtaat agatctcttc aacaacgaag ttatttctta cagcctttcg
      181 gaaagaccag tgatgaacat ggttgagaat atgctcgatc aggcattcaa aaagcttaat
      241 cctcacgagc atcctgttct gcactctgac cagggatggc agtatcgtat gagaagatat
      301 caaaatatcc ttaaagaaca tggtattaaa caaagcatgt ccagaaaagg caattgtctg
      361 gataatgctg tggtggagtg tttctttgga accttaaagt cggagtgttt ttatcttgat
      421 gagttcagta atataagcga actgaaggat gctgttacgg aatatattga atactataac
      481 agcagaagaa ttagcctgaa attaaaaggt ctgactccaa ttgaatatcg gaatcagacc
      541 tatatgcctc gtgtttaact gtccaacttt ttggggtcag tacaaagtga ggctacccta
      601 tgcctgaaca ccataaggtt agcctcttac tcgttggaaa tcaacacagg gggctgggaa
      661 tgccgcagaa atatagatta ctttctttaa tagtgatttg tttcacgctt ttatttttca
      721 cctggatgat aagagattca ctgtgtgaat tgcatattaa acaggagagt tatgagctgg
      781 cggcgttttt agcctacaaa ttgaaagagt aagagtcttc ggcgggaaat tattcccgcc
      841 ttacttacgg cgttgcgcat tctcattgca cccatattta ttcttcacaa aaataataat
      901 agattttatt acgcgatcga ttatttattt cctgaaaaaa aataaaaaaa tccccgccaa
      961 atggcaggga tcttagattc tgtgctttta agcagagatt acaggctggt tacgttacca
     1021 gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca ctttctgacc ttcgtccaga
     1081 gatttgtaac catcgttctg gatagcagag aagtgtacga acacatcttt agagccatcg
     1141 tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc attttacgat accagtcatt
     1201 ttaccggaca tagtgtatta cctttaataa ttaagtgtgc ctttcggcga tatggcgtgc
     1261 tttacagatt ttgaagcgtt aaaggaatgt gcactacgag gggtatcaac gataactctt
     1321 gaagggactt gccttactac actgctttaa tggtctgtac gtcaaaccgt tgatgtgcat
     1381 taagccactc attggcgggt gatgcaacaa ttatttttca tatttatgat taatcggttg
     1441 gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag tggataaaag tctatttcat
     1501 caactgctta cttaatgccg ggttggcttg aatcaaacgc atcaatttta gttcggcact
     1561 tgaaggcttc acgcgtctgg attcccattc ctttaccatg gcgactgata cgcccaaaac
     1621 ccgggcgaaa tcatcgattt ttaatcctgt cccttttcat aactgctcaa tttcggtaca
     1681 ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca tctttaaaaa caatctgttc
     1741 caggctgctc aacagctcat gcattggatc tttatattcc attgaaaact cctcaaatca
     1801 cactgcggga tcgtgaattg catcgaagct cattaagaat agtcgggaaa agcagacaca
     1861 gcggggtgaa gtcagggcat gctctggcta tgattcgatt cagcctgtca tttagcgtga
     1921 cttatagcca cctaacgatc tgataaatgc cgatatatct cttatcactc ctgttttgta
     1981 aagatttcgc aaatctaacc ttctgcgaag gcgtaaatct ctggctacaa aaaaggcaac
     2041 ggcactatca ttttttaaca atgaaacaac aaattaacct tgtttcattt tctccatgat
     2101 tttaccagga agattacgta gaaagggtat cctgcaagcc tgtcctggac tatccttgtc
     2161 acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt gaaaggagta agaaaatggc
     2221 gacaggaaag tcctgctctc gctggtttgc gcctcttgcg gcgttattaa tggtagttag
     2281 cctgagtggg tgttttgata aagaaggcga tcagcgtaaa gcgtttatcg acttcctgca
     2341 aaatacagtg atgcgtagcg gtgaacgtct accaaccctg actgccgatc agaaaaaaca
     2401 gtttggtcct tttgtctctg attacgcgat tctgtatggt tattctcagc aggtgaatca
     2461 ggcgatggat tccggtctgc gcccggtcgt agacagcgtt aacgcaattc gcgtaccgca
     2521 ggattacgtt acgcaaagcg gtccgctgcg tgaaatgaat ggttcgctgg gtgtgctggc
     2581 gcaacaactg caaaatgcga agttgcaggc cgatgctgcg cactctgcgc taaaacagag
     2641 cgatgacctg aaaccggtat ttgatcaggc attcaccaaa gtggtaacga cgccagctga
     2701 tgcattgcaa ccgttgattc cggcggcaca aactttcacg caacaactgg tgatggttgg
     2761 agactacatt gctcagcaag gtactcaggt gagctttgtc gcaaatggca tccagttccc
     2821 gacttcacag caggcaagtg aatataacaa actgattgcg ccattaccgg cacaacatca
     2881 ggcgtttaat caggcctgga ctacagcagt tactgcaacc caataaagag taaaagcccg
     2941 agcgattatc gggctttact tcacgcagtc gcagcttagt ccgcgacgtg aggattcaca
     3001 cagttcttct caacctttcc ttgtaacgca tcaatcaaat tatccacggc acaggcggcc
     3061 atgccataac gcgtctcatg ggtggcagat ccaatatgcg gtactgcgac gacgttggcc
     3121 attgagagca acggcgaatc tacggacagt ggctcttgtt cgaagacatc cagcccggcg
     3181 gcgtgaattt cgcctttctg caatgctgcg atcagtgcat tttcgtcaac caccgggcca
     3241 cgtccggcat taatgaaaat ggcggaggat ttcattttgg cgaattgttc tgcgccaaac
     3301 agatgatgcg tctcatcagt taacggcagg atcaggcaaa cgaaatctga ctcttgtaac
     3361 agagtatcca aatcgcagta gcgggcgttg aagcgttctt ctgcttcttt atggtggcgg
     3421 cgcgcgttat agaggatggg catgttgaag ccaaagtgca cacgttgtgc cagcgccatg
     3481 ccgatccgtc ccatcccgac aatgcccagt gttttatggt gaacgtcagt gccgtaccag
     3541 tccgggccta tgctcgcggt ccattcgcct gcttttaccc gttctgctac ctccacaacc
     3601 cgacgagcgg tagacaacac cagcgccatc agcgtatcgg cgacggtttc tgttaatacg
     3661 gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg catcgacatc aaaattgtca
     3721 tagccgacgg agatcgttga tgtggcacgc agtttcggca ttttttccag caatgcggca
     3781 tctacattct cgtttgaacc cagtaaacct tcagcttctg caaaaattgc tgcattttga
     3841 acgacggttt gtgggctgag gtttgccacc tggtgaacgg tgaaatgcgc ttgcaggcgt
     3901 tgcagtaaat catcaggtaa ggctttgtag aggataacgg acggcttcat gcttctctcc
     3961 attcactgat aaggcgatca ccttagcctg aaatatgatg aaggaaaact gaagaatgcc
     4021 aggtgatttc tgcatcacct ggcatgaaag ggattacagc gggcttaagg taatttctac
     4081 acggcggttt tgcgccttac cttctgcggt gctgttgctg gcgattgggt tagccgggcc
     4141 aaggccctga gtacggatgc ggctggcgtc cacgccctgg gtgatcaacg cgctggcaac
     4201 ggaatccgca cgctgctggg agagacgcat gttcaggtcg tgaccccccg tgctgtcggt
     4261 ataaccaatc acgttaaccg ccgttttcgg atactctttc agcaccattg ccacgccggt
     4321 cagggtgtta gcgcctgccg gtttcagggg agcgctgctg ctgtcgaagg tcacattgtt
     4381 cggcatattg aggataatgt tatccccgct gcgggttacg ctaacaccag tgccgcgcat
     4441 tttgtcacgc agcttcgctt cctgcacatc catgtaataa cccacgccgc cgcccagagc
     4501 tgcgcctgct gctgcgccaa tcagcgcgcc tttaccgcga tctttcttcg aagaagagag
     4561 cgcaccaata cccgcgccca cgagagagcc cagacctgcg ccgatagcag atttgcctgc
     4621 ttcgcgttcg ccggtgtaag ggttagttgt gcagccagat accgccagag cgccactcac
     4681 tacggcggca agtaagataa acacgtttct tcattgttaa tccttaataa cctttttatt
     4741 ctttgtcacg ggttccgtgg cgggagatta tgccgcgtga acatgaagat tattcctggg
     4801 aatactcgga aatttgtaag taatatttaa ctgctcaata catctaacct ttcaggagtc
     4861 ttcggtttgg ccaactcatc ctcacgatat tccgttctga ctgccgccca ctgggggccc
     4921 atgctggttg aaaccgacgg cgaaaccgtg tttagctcgc gtggcgcgtt agccacagga
     4981 atggaaaact ccttgcagag cgcggttcgc gaccaggttc acagcaatac gcgggtacga
     5041 tttccaatgg tgcgcaaagg ctttctcgcg tcaccggaaa acccgcaagg cattcgtggg
     5101 caggatgaat ttgttcgcgt gagttgggat gaggcgctgg atcttattca ccaacaacat
     5161 aaacgcattc gtgaggctta tggtccggca tcgatttttg ctggttccta cggctggcgt
     5221 tcaaacggcg tgctgcataa ggcctcgaca ttattacaac gctatatggc gctggcaggc
     5281 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     5341 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     5401 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     5461 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     5521 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     5581 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     5641 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     5701 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5761 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5821 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5881 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5941 ctttcttacc attttgccaa tggtggtaac cccacgcggc gttctgcggt gctctcttcc
     6001 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     6061 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     6121 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     6181 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     6241 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     6301 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     6361 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     6421 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     6481 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     6541 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     6601 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     6661 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6721 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6781 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6841 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6901 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6961 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     7021 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     7081 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     7141 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     7201 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     7261 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     7321 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     7381 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     7441 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     7501 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     7561 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     7621 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     7681 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7741 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7801 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7861 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7921 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7981 gaactgatga aagcaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     8041 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     8101 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     8161 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     8221 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     8281 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     8341 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     8401 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     8461 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     8521 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     8581 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     8641 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     8701 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8761 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataatcagca
     8821 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8881 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8941 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     9001 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     9061 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     9121 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     9181 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     9241 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     9301 tactatcatt acccagtttg cgctggggtc ggtttatacc tggagcctgt ttaatggcgc
     9361 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     9421 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     9481 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     9541 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     9601 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     9661 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9721 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9781 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9841 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9901 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9961 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
    10021 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
    10081 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
    10141 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
    10201 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
    10261 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
    10321 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
    10381 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
    10441 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
    10501 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaacag
    10561 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
    10621 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
    10681 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10741 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10801 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10861 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10921 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10981 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    11041 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    11101 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    11161 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    11221 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    11281 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    11341 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    11401 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    11461 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    11521 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    11581 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    11641 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    11701 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11761 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11821 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11881 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11941 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    12001 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    12061 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    12121 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    12181 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    12241 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    12301 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    12361 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    12421 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    12481 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    12541 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    12601 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    12661 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12721 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12781 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12841 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12901 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12961 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    13021 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    13081 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    13141 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    13201 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    13261 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    13321 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    13381 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    13441 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    13501 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    13561 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    13621 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    13681 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13741 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13801 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13861 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13921 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13981 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    14041 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    14101 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    14161 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    14221 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    14281 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    14341 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    14401 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    14461 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    14521 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    14581 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    14641 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    14701 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14761 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14821 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14881 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14941 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    15001 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    15061 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    15121 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    15181 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    15241 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    15301 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    15361 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    15421 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    15481 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    15541 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    15601 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    15661 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15721 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15781 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15841 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15901 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15961 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    16021 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    16081 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    16141 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    16201 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    16261 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    16321 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    16381 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    16441 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    16501 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    16561 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    16621 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    16681 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16741 gtggtcgata tcatgctggc gctgccaagc ctgctgttgg cactggtgct ggtggcgatt
    16801 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16861 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16921 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16981 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    17041 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac tatgctctcc
    17101 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    17161 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    17221 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    17281 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    17341 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    17401 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    17461 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    17521 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    17581 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    17641 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    17701 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17761 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17821 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17881 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17941 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    18001 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    18061 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    18121 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    18181 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    18241 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    18301 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    18361 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    18421 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    18481 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    18541 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    18601 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    18661 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18721 cagcgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18781 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18841 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18901 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18961 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    19021 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    19081 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    19141 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    19201 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    19261 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    19321 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    19381 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    19441 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    19501 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    19561 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    19621 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    19681 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19741 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19801 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19861 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19921 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19981 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    20041 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    20101 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    20161 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    20221 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    20281 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    20341 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    20401 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    20461 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    20521 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    20581 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    20641 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    20701 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20761 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agca
//
LOCUS       DAJVRL010000071        20699 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVRL010000071 DAJVRL010000000
VERSION     DAJVRL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.071"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            81..350
                     /locus_tag="OQN02_002629"
     CDS             81..350
                     /locus_tag="OQN02_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1643371.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="OQN02_002630"
     CDS             344..709
                     /locus_tag="OQN02_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1643372.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OQN02_002631"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OQN02_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1643373.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="OQN02_002632"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="OQN02_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1643374.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="OQN02_002633"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="OQN02_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1643375.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="OQN02_002634"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="OQN02_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1643376.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OQN02_002635"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OQN02_002635"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1643377.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OQN02_002636"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OQN02_002636"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1643378.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKLADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="OQN02_002637"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="OQN02_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OQN02_002638"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OQN02_002638"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1643379.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OQN02_002639"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OQN02_002639"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1643380.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14857..16455
                     /gene="rtcR"
                     /locus_tag="OQN02_002640"
     CDS             14857..16455
                     /gene="rtcR"
                     /locus_tag="OQN02_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS1643381.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16437..17195)
                     /locus_tag="OQN02_002641"
     CDS             complement(16437..17195)
                     /locus_tag="OQN02_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1643382.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="OQN02_002642"
     CDS             complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="OQN02_002642"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1643383.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="OQN02_002643"
     CDS             complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="OQN02_002643"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1643384.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18603..20108
                     /gene="glpD"
                     /locus_tag="OQN02_002644"
     CDS             18603..20108
                     /gene="glpD"
                     /locus_tag="OQN02_002644"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1643385.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20377..20607)
                     /locus_tag="OQN02_002645"
                     /pseudo
     CDS             complement(20377..20607)
                     /locus_tag="OQN02_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4889 a         5692 c         5218 g         4900 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gctaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg caccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttttt aatttattgt
    14761 ttctaaaaag attatatctt tatgtccgta aacggagatt tcccgcaaag ccgatttacc
    14821 gataatgaaa tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
    14881 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt
    14941 ccgtcactct gtatatacca gcaagaatcg ttggtcatcg atcgactgga attattgcac
    15001 gatgcccgct cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca
    15061 gaaacagaag tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
    15121 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
    15181 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
    15241 gcgcgttatc tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg
    15301 cgcggcccag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
    15361 agccgctttg ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg
    15421 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc
    15481 gcgccgattc tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc
    15541 ttcgagttaa aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
    15601 accctgcgcg gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc
    15661 ggggcgcggg aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt
    15721 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag
    15781 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
    15841 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac
    15901 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
    15961 gaaccgaacc tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt
    16021 tttaacaccg aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggcaacatgg
    16081 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    16141 ggacgcatca ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag
    16201 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
    16261 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
    16321 gctgccggac gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
    16381 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
    16441 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
    16501 gcgtcggtgt agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg
    16561 ccaaattttg aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc
    16621 ttggtacgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg
    16681 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
    16741 atgatcccgc catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct
    16801 tttaccatca acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga
    16861 ttgagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
    16921 ccattgggga tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
    16981 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga
    17041 tggcgcagga tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg
    17101 ctgacggaga agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt
    17161 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
    17221 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat
    17281 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    17341 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
    17401 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    17461 cccgctgaat ttttgctgca tatagccgct taacagggcg ctgataagcg taatgacaat
    17521 tagcttaccg ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag
    17581 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    17641 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
    17701 gagaatttgc atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg
    17761 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
    17821 ggtatggcct gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
    17881 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
    17941 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
    18001 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
    18061 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt
    18121 cgctgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
    18181 agatactgcg ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg
    18241 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
    18301 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    18361 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    18421 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
    18481 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
    18541 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
    18601 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    18661 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    18721 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    18781 aattccgcct ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
    18841 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
    18901 tgattcgcat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
    18961 caactggttt gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg
    19021 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    19081 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
    19141 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
    19201 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
    19261 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc
    19321 atacccaaaa gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc
    19381 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga
    19441 aagcagtgaa gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact
    19501 ttaaaaagca gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
    19561 gtgatgatga gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg
    19621 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    19681 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    19741 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg
    19801 ctcgcctgcg ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacacgcgca
    19861 cttacggcag caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg
    19921 aagatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    19981 tccgccgcac cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    20041 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    20101 cgtcgtaaat taacgtaagg tgatcaggtc agatttcaat ctggcctgag actgatgaca
    20161 aacacaaaac tgcctgatgc gctacgctta tcaggcctac gtggtttatg caatatattg
    20221 aatttgcatg gtcttgtagg ccagataaga cgttcacgtc gcatccggca tgaacaaagc
    20281 gcactttgtc aaaaatctaa cctacttttt aattcaggga attaccgcaa agcccacgcc
    20341 atcataccca acgtaacaaa actcaggcac gttccccact cgccccgaga aaaatagcat
    20401 taatgcgccc agcgtcagca taaaaatatt gagcagaagg taaattgttg gcgtaaagag
    20461 taaattgcaa acctcaggaa tagcggcaag cccagccccc agcatggcaa taaccgctgc
    20521 aaaaaattgc gtcagtattg ggatgccgaa aaaagtcatt accagcgagg tcaaaatcca
    20581 ttttctattt tgcattattc tttccattct ttctgaatgg cgaaattata ctccccgagt
    20641 ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg accctttgg
//
LOCUS       DAJVRL010000072        20569 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVRL010000072 DAJVRL010000000
VERSION     DAJVRL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.072"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            409..1239
                     /locus_tag="OQN02_002646"
     CDS             409..1239
                     /locus_tag="OQN02_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1643386.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OQN02_002647"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OQN02_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1643387.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OQN02_002648"
     CDS             complement(2368..3828)
                     /locus_tag="OQN02_002648"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1643388.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OQN02_002649"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OQN02_002649"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1643389.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OQN02_002650"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OQN02_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCS1643390.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OQN02_002651"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OQN02_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCS1643391.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OQN02_002652"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OQN02_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCS1643392.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OQN02_002653"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OQN02_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCS1643393.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OQN02_002654"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OQN02_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCS1643394.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OQN02_002655"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OQN02_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="OQN02_002656"
     CDS             complement(12470..13009)
                     /locus_tag="OQN02_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1643395.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OQN02_002657"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OQN02_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1643396.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14102..14698)
                     /gene="fimE"
                     /locus_tag="OQN02_002658"
     CDS             complement(14102..14698)
                     /gene="fimE"
                     /locus_tag="OQN02_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCS1643397.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15176..15778)
                     /gene="fimB"
                     /locus_tag="OQN02_002659"
                     /pseudo
     CDS             complement(15176..15778)
                     /gene="fimB"
                     /locus_tag="OQN02_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17232..17948
                     /gene="nanC"
                     /locus_tag="OQN02_002660"
     CDS             17232..17948
                     /gene="nanC"
                     /locus_tag="OQN02_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1643398.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17968..19074
                     /gene="nanM"
                     /locus_tag="OQN02_002661"
     CDS             17968..19074
                     /gene="nanM"
                     /locus_tag="OQN02_002661"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1643399.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19139..20119
                     /locus_tag="OQN02_002662"
     CDS             19139..20119
                     /locus_tag="OQN02_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1643400.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5478 a         4908 c         4672 g         5511 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 ttgtcgtttt ttaatatttt tatgcttgag aaaaaatacg taacttattt atgatatgga
    14041 cagtttggcc ccaattgtct tgtgtttatt tgttttttaa atttattatc aataagttaa
    14101 atcaaacctc ttctcttttt aatttttcgt ttatgagatt atttctttcc cataatccgg
    14161 caaaacgagc agcattactg gcggtataac gcacagtatg gcgaatattt cgattcccga
    14221 gataatcctg aattaaacgg gtatctgcac cacgctccgc cagttcataa ccgcaagcat
    14281 gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg gcatcgcgaa
    14341 taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata aatatagcgt
    14401 cggtccggtc agcgcctttc cagttagcac gttcctgggt ccagcgttcc acggcttcac
    14461 gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg cgaatattta
    14521 ttcgaccttc attaaggtca aggtcctgat aatgcagatc aagcagttca ctaatacgca
    14581 tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt gccccgtaac
    14641 aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt ttactcacaa
    14701 tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc tcaatcggtc
    14761 aaaattagcc caatagataa aaagataacc acacacgaag ttatgtggct atcagtcaat
    14821 tacattaaac atcatacaaa aataaaatat cactgatgtg acaacatcta ttttcattga
    14881 tttgattcaa tataacaata gattacagag gattaacttc cagttactta aaaaataaag
    14941 aaaaaagtgt ggaaacagga gaatttaagt gatgtgtaaa cttatgaatg attttgttta
    15001 tttattttga agaacataaa ccattagatt ttgtatgggt atggttaact taatgtttat
    15061 tatgataatt aagaatcaca cttacaataa catcaattaa taaatggtta atcatttttg
    15121 atatatcgta agaataatgt agtttttaac accatccctg gtatctcaac tatctctata
    15181 aaacagcatg acgctgtcgt cctctggctc tatcccagat gccgtaaaaa cgccctgcat
    15241 tgctggcggt ataccagaca gtatgacgaa tattgcgatg cccaagataa tcctagataa
    15301 gtcgcgtatc tattcccata ttcgccaaag caaaaccaca cgaatagcgt aacatgtgcg
    15361 gatgaatctc cagtgacaac ccggcattac caccagaagt cgagataata tggtaaaact
    15421 gttgccgaga aagcggattc cccttacgtg ataaaaatac ccactcgctc tcagcatgcg
    15481 gataagaagt acggatactc aaccagtttt ttaaagcctg aacttctttg ttcaatagcg
    15541 ggtgcgttgt tgaaaagcct ttttttaatc gatggatata tatacacttt gctttaagat
    15601 caatatccga aatcctcaat cgacaaattt cactcgcccg gaaaccatga ataaaacaaa
    15661 gcaaaatcag acaataatta cgtgttgcat gaggcccggt atttgctgct ttaaggagtg
    15721 actcgatttc actatgggta aggaagttcc tttttttgtt atcagcctta ttcctcatcg
    15781 ttttccctta taattacaga cgcgcactag ctgtgctggg ttagcaataa tccaacattt
    15841 ttatcctgat tatgtttata aaagcgaacg tttgcttaat aacttacgta agtggaccag
    15901 ttcttctgag cgaacttaaa tggagtagcg atagttactt ataataactt tgccatacaa
    15961 acgccatgct cttgcatgct atgtaccttt atatatttat caatctgata cgaatctaat
    16021 aataaccctc atcattaatg caactaatct tatctatata tcatgtgata tgttctgtaa
    16081 caagtaaaat caacataaaa aattcagcaa aatttaatat ttgttaaact agccgtctca
    16141 aatatgacat tattagttaa ctgcatttgc cacaaaatgg ctatttcatg gaacgcagta
    16201 atttcttagt ggctattatc atgctaattc ttagatccct agcgattatt cctgcctggt
    16261 tcgtcagctt aggccatgag attgttacat tcctttttat cctggagata tgggggaacg
    16321 caggcgacgc tttttcatct acatccgttg gtaaatatag ctttggtcac tacgacttaa
    16381 gccttcctca atcacagcta ttcgccgggc gatacggcaa acaaactaag tgaaacatca
    16441 tcctgaaaac accaacatca acaagcctct ccagatcgac atcaggagtg accagttaca
    16501 agccacaaac caagaaacat atttcatgat gagaattatg ctcagcctga tggcgagtaa
    16561 acccttattt tcaccactct cactgagtta gcttgcaaag ctttctttgt ttagattcta
    16621 aataaattat ttataaaaca tatagttaaa agaaaatctt tagtttttga agaccaccgc
    16681 aagtgttcgt ctggcttcac atggcatctt cttcttagaa aaagatcgac atattttgtg
    16741 acgcgaattg caaatctggt tttgttgtat ggattgcgtg atttttgatc tggtataaca
    16801 ggtataaaga tgcaccacga tagtcaatga gactgggcat ctcgtaatct atggcaaaca
    16861 tcacttcagt tctttcacat cgggtgatga aaatgcactt cagtctgaaa ggaatatgaa
    16921 aatgagatca acagacactc tattttatga ctctgggtaa aatgggttga gtaagtgata
    16981 taacttacga atattcaaat caattaaaaa tcagaagaga ttttatactc aggtatttaa
    17041 tctggatctc tgtttattta aataatgtta aaagagaatt ttcacaggag accttataca
    17101 aaaaatataa aatacagtta cccgttgcca aagacactat aagcctggca aaaaaatatt
    17161 acacaacata aatgctaatt gtttatgcgg gctttgtatt gctttctgta tcctacaaat
    17221 gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt attattactg tgcttttcgt
    17281 ccccattaat ttctcaggct gcgacactgg acgtacgtgg tggatatcgt agtggaagcc
    17341 acgcctatga gactcgactc aaagtcagtg agggatggca aaatggatgg tgggcaagca
    17401 tggaaagtaa tacctggaat accattcatg ataataaaaa ggaaaatgcc gcactcaatg
    17461 atgttcaggt tgaagttaat tacgcaatta aacttgatga tcaatggacg gtgcgcccgg
    17521 gaatgttaac gcattttagc agcaacggca cacgctacgg accctacgta aaactgtcct
    17581 gggacgcgac aaaagatctt aattttggca ttcgctatcg ttacgactgg aaagcttacc
    17641 gacaacaaga cttatccggt gatatgtctc gtgataacgt tcatcgttgg gatggatatg
    17701 tcacttacca tattaatagt gatttcacct tcgcatggca aacgacgcta tacagcaaac
    17761 agaatgatta tcgctatgca aaccacaaga aatgggcgac ggaaaatgcc tttgttctac
    17821 aataccatat gacgcccgat attacgccat acatagaata tgactacctt gaccgccagg
    17881 gtgtttacaa cggcagagat aatttatcgg aaaacagtta tcgcattggt gtgtcattta
    17941 aactgtagta gacaggagac agtcacaatg aataaaacta taatggcgct tgctatcatg
    18001 atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa ctcctgtgcc atttaaaagt
    18061 ggtaccggag caattgataa cgacactgtc tacattggtt taggtagcgc aggtacagca
    18121 tggtacaagc tagatacaca ggccaaagat aaaaaatgga ctgcgttagc tgcattccct
    18181 ggtggaccga gagagcaagc aacctctgca tttattgatg gcaatctgta tgtgtttggc
    18241 ggcattggca aaaacagcga gggattgact caggtattta atgacgtaca caaatacaac
    18301 cccaaaacca atagctgggt taaattgatg tcgcacgcgc cgatgggcat ggcgggtcat
    18361 gtgacttttg tacacaacgg caaggcttat gttactggcg gtgttaacca gaatatcttc
    18421 aatggctatt ttgaagatct caacgaggct ggaaaagatt caaccgctat agataaaatc
    18481 aacgcccact attttgacaa aaaagcagaa gattatttct tcaataagtt tctgttgtct
    18541 tttgatccct caacacagca atggagttac gctggcgaat cgccctggta cggaacggct
    18601 ggtgcggcgg ttgtgaataa aggtgataaa acctggctta ttaatggcga agccaaacca
    18661 ggattgcgaa cggatgccgt atttgaactt gatttcaccg gtaataattt aaaatggaat
    18721 aagcttgatc ccgtctcatc accagatggc gtcgctggcg gttttgcggg gataagcaat
    18781 gattctctta tatttgccgg aggggccgga ttcaaaggtt cacgagaaaa ttaccagaac
    18841 ggtaagaact atgcgcatga aggcctgaaa aaatcatata gcactgatat tcatctttgg
    18901 cataacggga aatgggataa atcgggtgaa ttatcgcaag gtcgggccta cggagtatca
    18961 ttgccctgga ataatagttt attaattatt ggcggtgaaa ctgcaggcgg caaagcggtg
    19021 acggattcag ttttaatttc tgtgaaggat aataaagtca cagtacaaaa ctaacgcttc
    19081 agggccccgg taaaggggcc ctgctatcaa tttgtcattt gtattacgcg aaaattatat
    19141 gaacgcaata atatcacccg attattacta tgttcttacc gttgctggtc agtctaatgc
    19201 catggcgtat ggcgaaggac tgccattacc ggacagggaa gatgcgcctc attccagaat
    19261 taaacaatta gcgagatttg cgcatacgca tcccggaggc ccgacatgtc actttaacga
    19321 cattattcca ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc
    19381 tctggcaacg aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg
    19441 gaaattactg cccttcattc ctgataatgc agggattctt atcgttccgt gttgccgtgg
    19501 cggatcggct tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca
    19561 tgatgcttgt cgttggggaa cggatactcc gctgtaccag gatttagtca gcagaacgcg
    19621 agccgcactg gcaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga
    19681 atttgactta atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga
    19741 agcctttcgt agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcgcc
    19801 gtggttttgc ggcgatacca cctggtactg gaaagaaaat ttcccgcatt cgtatgaagc
    19861 tatttatggc aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca
    19921 acaaggtgaa agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg
    19981 atattacggt tcagcgtacc ggtcaccgga gaactggacg acggcactgc gaagcagtca
    20041 tttcagcacg gcagcccgtc gggggattat ttctgacaga tttgtagaag caattttgca
    20101 gttttggcgc gaaaggtgag cggggagttt attcatgggg gatgtccttg ttcgttctca
    20161 gagtagacaa caaaaaacaa atgagatcaa ggtaatatct cttcacttgc gggctcgaac
    20221 agcaggcaat tgccaatata ataatctggc acaagggtga aaaacgcgca ccgcataaac
    20281 cgttgttatt gcttacagat tagccggatg ccttaataga catccgcgcc ttttcgatta
    20341 tggctcggaa atctacgaac atctacacag tttactggaa cgttttgggc cacagcgttc
    20401 gcagcaccgt cctgatatgc cgttctggcg attataagtc gacggattct ggcaaataga
    20461 cgatgatgat atgcttaatg atgttcaaag aaattaggcc attattatat tattgcttgc
    20521 attttctttc attcctaaaa gcacccgcca ttaatcccag gcgcattat
//
LOCUS       DAJVRL010000073        20537 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVRL010000073 DAJVRL010000000
VERSION     DAJVRL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.073"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            98..397
                     /locus_tag="OQN02_002663"
     CDS             98..397
                     /locus_tag="OQN02_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1643401.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            407..931
                     /gene="ygaP"
                     /locus_tag="OQN02_002664"
     CDS             407..931
                     /gene="ygaP"
                     /locus_tag="OQN02_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1643402.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(978..1382)
                     /gene="stpA"
                     /locus_tag="OQN02_002665"
     CDS             complement(978..1382)
                     /gene="stpA"
                     /locus_tag="OQN02_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1643403.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2050..2499
                     /gene="alaE"
                     /locus_tag="OQN02_002666"
     CDS             2050..2499
                     /gene="alaE"
                     /locus_tag="OQN02_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1643404.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2536..2880)
                     /locus_tag="OQN02_002667"
     CDS             complement(2536..2880)
                     /locus_tag="OQN02_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1643405.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3032..3262
                     /locus_tag="OQN02_002668"
     CDS             3032..3262
                     /locus_tag="OQN02_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643406.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3609..3854
                     /gene="nrdH"
                     /locus_tag="OQN02_002669"
     CDS             3609..3854
                     /gene="nrdH"
                     /locus_tag="OQN02_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1643407.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3851..4261
                     /gene="nrdI"
                     /locus_tag="OQN02_002670"
     CDS             3851..4261
                     /gene="nrdI"
                     /locus_tag="OQN02_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCS1643408.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4234..6378
                     /gene="nrdE"
                     /locus_tag="OQN02_002671"
     CDS             4234..6378
                     /gene="nrdE"
                     /locus_tag="OQN02_002671"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1643409.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6388..7347
                     /gene="nrdF"
                     /locus_tag="OQN02_002672"
     CDS             6388..7347
                     /gene="nrdF"
                     /locus_tag="OQN02_002672"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1643410.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7702..8904
                     /gene="proV"
                     /locus_tag="OQN02_002673"
     CDS             7702..8904
                     /gene="proV"
                     /locus_tag="OQN02_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1643411.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8897..9961
                     /gene="proW"
                     /locus_tag="OQN02_002674"
     CDS             8897..9961
                     /gene="proW"
                     /locus_tag="OQN02_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1643412.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10019..11011
                     /gene="proX"
                     /locus_tag="OQN02_002675"
     CDS             10019..11011
                     /gene="proX"
                     /locus_tag="OQN02_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1643413.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11203..12382
                     /locus_tag="OQN02_002676"
                     /pseudo
     CDS             11203..12382
                     /locus_tag="OQN02_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12506..13243
                     /gene="ygaZ"
                     /locus_tag="OQN02_002677"
     CDS             12506..13243
                     /gene="ygaZ"
                     /locus_tag="OQN02_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1643414.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13233..13568
                     /gene="ygaH"
                     /locus_tag="OQN02_002678"
     CDS             13233..13568
                     /gene="ygaH"
                     /locus_tag="OQN02_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1643415.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13659..14189
                     /gene="emrR"
                     /locus_tag="OQN02_002679"
     CDS             13659..14189
                     /gene="emrR"
                     /locus_tag="OQN02_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1643416.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14316..15488
                     /gene="emrA"
                     /locus_tag="OQN02_002680"
                     /pseudo
     CDS             14316..15488
                     /gene="emrA"
                     /locus_tag="OQN02_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15505..17043
                     /gene="emrB"
                     /locus_tag="OQN02_002681"
     CDS             15505..17043
                     /gene="emrB"
                     /locus_tag="OQN02_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1643417.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="OQN02_002682"
     CDS             complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="OQN02_002682"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1643418.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="OQN02_002683"
     CDS             complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="OQN02_002683"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1643419.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19401..19829)
                     /locus_tag="OQN02_002684"
     CDS             complement(19401..19829)
                     /locus_tag="OQN02_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1643420.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="OQN02_002685"
     CDS             complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="OQN02_002685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS1643421.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4954 a         5254 c         5129 g         5200 t
ORIGIN      
        1 tcagcttacg cttttactct catttacgct gctgaggctg gtatatgttg catttttagt
       61 ttagagttta cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca
      121 ggccagtacc gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct
      181 gattctgtgc atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac
      241 cggactgagc gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat
      301 caacagccaa cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc
      361 cattatcgcc accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac
      421 catttcgccg catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg
      481 tgacgctgat gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt
      541 gctggaacag tcaggtcttc cacctaaatt acgtcgcgag caaattatat tccactgcca
      601 ggcaggtaaa cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga
      661 aatattttta ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt
      721 aaataaatct caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat
      781 attaatcggc gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt
      841 tgttggtgcc gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt
      901 agataagatg ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg
      961 acatccggcc tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc
     1021 tgagcaattg gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca
     1081 tcagtgaatt tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca
     1141 gcagagctat tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc
     1201 caggtgctaa ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca
     1261 cgtctttctt tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag
     1321 aattcgcgag ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac
     1381 ataataaaga aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg
     1441 atcacttatt atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca
     1501 gctgttattt tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga
     1561 gtaatttagg ttattggtga acagagtggc gctctctgct cgctaactcc gcaagcctga
     1621 caaaaccgaa gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt
     1681 gctttctgct gttaatgtac attttctggc atcaaaaatg ctgtaaatga cccggtcacg
     1741 acaaacaccg aattctctac gcttatagcg ttacctcccc cccaaacttt cattagtgaa
     1801 attaaaggca agatcatatc taattgattt aattgaaaat aaatcatatc ggcatgtaaa
     1861 aatccacaat gtacaaaaaa cgagccgtta cggaatattt tatctacaaa aactaactaa
     1921 ataaaaattt ttcactaatt gattagtcat agacagcgat atacgctatg caaaaatgca
     1981 gatggcaatg agatccactg ctttcatctc cattaacatc ccattacgct tttattaagg
     2041 agcattagca tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga tacgttcgcg
     2101 atggttgttt actgttctgt cgtgaacatg tgtattgaag ttttcctctc cggaatgagc
     2161 ttcgaacagt ctttttattc cagattggta gcgattccgg tgaacatctt aattgcatgg
     2221 ccatacggta tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag cccgtcgggc
     2281 tggataaaaa atctggctga tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg
     2341 gcgatcttgt tagtggtggg cgcagactgg catcagatta tggcggcggt cagttcaaac
     2401 atcgttgttt cgatgttgat gggggcggtt tatggctact tcctcgatta ttgccgccga
     2461 ctgtttaaag tcagccgtta ccagcaggta aaagcctgac tcttcttttc gcgactggca
     2521 tcgccagtcg cgtctttaac tggcttcgcc aaataaacca ttcaaataac gttcaagcgc
     2581 aacacgcgaa ctaaagccat gcgggatacc gtaatgctcg tgtcgctcgc ctgctaaata
     2641 gagcggaaat tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt cagccagtgt
     2701 tgaactgcgt tcttttaatg ttggatgaat taccagagcg gtacgtccca ttcgcgcttc
     2761 gcgattaaca tagacataat tttccccacg gcggtaacca tacgctttct gggttaccac
     2821 atcgacagta aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc gtaaatacat
     2881 atttgatcct cgttattttc aaccgcgggc ctaccttacc tgattgcgca ttcacaacgc
     2941 tttcagaaaa gtccataaag ccgcgatgaa cctcagttaa gaaaattatg gtctacactg
     3001 aaaattacat cgaattctaa tgggggatca tatgtataac cgaccgaacc gcaacgacgt
     3061 tgatgatggc gtgcaggata tccagaatga tgtcaatcaa ttagctgaca gcctcgaatc
     3121 tgtattgaag tcctggggca gcgacgccaa aggggaagct gaagccgcac gcagtaaagc
     3181 tcaggcgttg ctgaaagaaa ccagggcgcg aatgcatggt cgtactcgcg tccagcaagc
     3241 cgcgcgcgat gccgttggct gagcggattc ttttgttcgt gaaagaccct ggtgtagcgt
     3301 gggtacagca gctgcggtag gtatttttat cggcgcactg ttaagcgtgc gcaaatcgta
     3361 gtgcaaaaat gataataaat acgcgtcttt gaccccgaag cctgtcttcg gggtttcttt
     3421 ttgcctggtg aatcacaaaa atccccctcc cccgtcacgc tcatatccag ggtaatttcg
     3481 atcactattt gctatatatt gtgtggttga atcttttttc aactacatct agtatctctg
     3541 tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt atcttcattt taaacggaaa
     3601 tacgaatcat gcgcataact atttatactc gtaacgattg cgttcagtgc cacgccacca
     3661 aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat cgcgttcctg
     3721 aagcagcgga agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg attgctggcg
     3781 atcttagctg gtctggtttc cgtccggaca tgattaaccg tctgcatcca gcgccacacg
     3841 cggccagtgc atgagccagc tcgtctactt ctccagcagc tccgaaaaca cgcagcgttt
     3901 tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg aacggattca
     3961 ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta cggctggcgc
     4021 ggtgccacga caggtaattc gctttttaaa cgacgagcac aaccgggcgt tgcttcgcgg
     4081 cgttattgct tctggtaatc gcaactttgg tgaggcgtat ggccgcgccg gagatgtgat
     4141 tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta cgcaaagcga
     4201 tatcgaaaac gttcgtaaag gagtaaccga attttggcaa cgacaaccgc agaatgcctg
     4261 acgcaggaaa cgatggatta ccacgcgctg aatgcgatgc ttaatcttta cgatagcgca
     4321 ggtcgcattc agttcgataa agaccgccag gccgttgacg cctttattgc gacgcatgtg
     4381 cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc tgaactggct ggtcaacgaa
     4441 ggttactatg atgaaagcgt tcttaatcgc tactctcgcg actttgtcat tacgctgttt
     4501 gcccacgcac acaccagcgg ttttcgtttc cagacattcc tcggggcatg gaagttttac
     4561 accagctata cgttgaagac attcgacggt aaacgttatc tggaagattt tgccgatcga
     4621 gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt gcaactgacc
     4681 gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa cattcctcaa ctgcggtaag
     4741 cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa tatggagtcg
     4801 attggtcggg cggtaaattc cgcactgcag ctgtcgaaac gcggcggcgg cgtagcattt
     4861 ttgctgtcga atctgcggga agcgggcgcg ccaattaaac gtattgaaaa tcaatcttct
     4921 ggcgtaattc cggtgatgaa aatgttggaa gacgcatttt cctatgccaa ccaactcggc
     4981 gcgcgtcagg gggcaggtgc agtctattta catgctcatc atcccgatat tctgcgtttt
     5041 ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact gtcgctgggc
     5101 gtggtgatcc cggatatcac tttccatctg gcaaaagaga atgcgcagat ggcgctgttt
     5161 tcgccctatg acgttgagcg agtttatggc aagccgtttg ccgatatcgc cattagcgaa
     5221 cactatgacg aactggttgc cgatgaacgc atccgcaaaa aatacctcaa cgcccgtgat
     5281 ttcttccagc gactggcaga aatccagttt gagtccggct atccctacat catgtatgaa
     5341 gacacagtaa accgtgctag ccctatcgcc gggcgcataa atatgagtaa tctctgctca
     5401 gaaattttgc aggttaacag cgcctcagag tatgacgaga atctcgacta tgcccgcaca
     5461 ggccatgata tttcctgcaa tttaggttcg ttgaatattg cgcacaccat ggattccccc
     5521 gatttttccc gcacggtaga gactgccgtg cgcggtttaa cggcggtgtc agatatgagt
     5581 catatccgca gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc catcggactg
     5641 gggcagatga atttacatgg ttatctggcg agggaaggca tcgcttatgg ttcgccggaa
     5701 gccctggatt tcaccaatct ctatttctat accatcacct ggcacgcact gcgtacctcg
     5761 atgttgctgg cgcgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc acgctatgcc
     5821 agtggtgaat atttcagcca atatctgcaa ggtaactggc agccgaaaac ggcgaaagtt
     5881 ggcgaactgt ttgcccgcag ctgtattacg ttacctaccc gtgagatgtg ggcgcaactg
     5941 cgcgacgacg tgatgcgcta cggcatatac aaccagaatc tccaggcggt gccgccaacc
     6001 gggtctatct cttatatcaa ccatgctacg tcgagtattc atccgattgt ggcgaaagta
     6061 gagatacgca aagagggcaa aacaggacgc gtttactacc ctgccccgtt tatgactaac
     6121 gaaaatctgg cgctgtatca ggacgcttac gaaattggcg cagaaaagat catcgacacc
     6181 tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt tttccccgat
     6241 accgccacca ctcgcgatat caacaaagcg cagatttacg cctggcgcaa aggtatcaaa
     6301 acgctctatt acatccgcct gcgtcagatg gcgctggaag gcactgaaat tgaaggctgc
     6361 gtctcctgtg cactttaagg aatatctatg aaactctcac gtatcagcgc catcaactgg
     6421 aacaagatat ctgacgataa agatctggag gtgtggaatc gcctgaccag caatttctgg
     6481 ctaccagaaa aggtgccgct gtcgaacgat attcccgcct ggcagacatt aactgtcgta
     6541 gaacaacaac tgacgatgcg cgtttttact ggcctgacgc tgctcgacac gctgcaaaat
     6601 gttatcggcg cgccttcgct gatgcccgat gcgctcacgc ctcatgaaga ggcggtatta
     6661 tcgaatatca gctttatgga agcggttcat gcccgctctt acagttcgat tttctcgacg
     6721 ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa cgcaccgttg
     6781 cagcgaaaag ctcagattat tcagcaacat tatcgcggtg atgatccgct gaaaaagaaa
     6841 atcgccagtg tgtttcttga atcttttttg ttctattccg gtttctggct gccgatgtat
     6901 ttttccagcc gcggaaagct gaccaatacc gcggacctga tccgtctgat tatccgcgat
     6961 gaagcagtcc acggttacta cataggctat aaatatcaga aaaacatgga aaagatatct
     7021 ctgggacaac gtgaagagtt gaagagtttc gccttcgatt tgttgctgga actctacgac
     7081 aacgagttgc aatacaccga tgagctgtac gccgaaaccc cgtgggctga cgatgtgaaa
     7141 gcgtttctct gttacaacac caataaggct ttgatgaatc tgggctacga accgttattt
     7201 cccgcagaaa tggcggaagt gaatccggca atcctcgccg cgctttcgcc gaatgccgat
     7261 gaaaatcacg atttcttttc cggttcaggc tcctcttatg tgatggggaa agcggttgaa
     7321 acagaagatg aagactggaa tttctgaggg tgttattttc aaaaatatca ctacccgcag
     7381 cagggaaata attcccgcca aatagctttt tatcacgcaa atattctgta gtgatctaca
     7441 ctgatactct gttgcattat tcgcctgaaa ctacaatatt caggcgtttt ttcgctatct
     7501 atgacaaaaa atatcaactt ttctcgattt gctctcagcc cttatatcac ggaaaattcc
     7561 ggcgatttgc tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct
     7621 cagtatgtta gggtagaaaa aagtgactat tcccattggg taatatatcg acataaacaa
     7681 ataaaggaat cattctattg catggccatt aaattaaaaa ttaaaaatct ttataaaata
     7741 tttggtgagc atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa
     7801 attctggaaa aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa
     7861 ggcgagatat ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt
     7921 ctcaatcgcc tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc
     7981 aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag
     8041 tcctttgcct taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg
     8101 gccggaatta atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg
     8161 ctggaaaatt atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg
     8221 ggattagccc gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg
     8281 gcgctcgatc cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa
     8341 catcagcgca ccattgtctt tatttcccac gatcttgatg aagccatgcg tattggcgac
     8401 cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc
     8461 aataatccgg cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggtg
     8521 ttcagtgcga aagatattgc ccgccggaca ccgaatggct taattcgtaa aactcctggc
     8581 ttcggcccac gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt
     8641 atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta
     8701 acgcagcagc aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa
     8761 acgcctctta gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc
     8821 gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat
     8881 cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga
     8941 cagtgccgcg caatccgcag acgcctgggg tacaccgacg actgcaccgg ctgacggcgg
     9001 tggtgctgac tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga
     9061 tccgttccat aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt
     9121 tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa
     9181 cggtttccag caattgctgc tgggtatgcc agcgccagtg gcgattatcg ttttcgctct
     9241 catcacctgg cagatttccg gggtcggaat gggcgtggcg acgctggtct cgctgattgc
     9301 catcggcgca atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaactgc
     9361 cctgctgttc tgtatcgtca tcggtttgcc gttggggata tggctggcga gaagtccgcg
     9421 agcggcgaaa attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta
     9481 tctggtgcca atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat
     9541 catctttgcg ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc
     9601 ggatctgatt gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt
     9661 tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc
     9721 cctttctatg gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact
     9781 tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat
     9841 cctcgccatt atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg
     9901 caaccgtcgc tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta
     9961 actctgcact tgcccggtgg cgccgggcat tatcaccctg ccaaaaaaag gaataacaat
    10021 gcgacatagc gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc
    10081 tgccgatctg ccgggcaaag gcattactgt taatccagtt cagagcacca tcactgaaga
    10141 aaccttccag acgctgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaacaa
    10201 acccagcgaa gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt
    10261 caccgccgtg aactggacgc cactccatga caacatgtac gaagctgccg gtggcgataa
    10321 gaaattttat cgtgaagggg tatttgttaa cggcgcagca cagggttacc tgatcgataa
    10381 gaaaaccgcc gaccaataca aaatcaccaa catcgcacaa ctgaaagatc cgaagatcgc
    10441 caaactgttc gataccaacg gcgacggtaa agcggattta accggttgta accctggctg
    10501 gggctgcgaa ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac
    10561 tcataatcag gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg
    10621 caaaccggtg ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg
    10681 gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga
    10741 taccaaactg ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc
    10801 caacaaagcc tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt
    10861 gccagtggcc gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg
    10921 cgatattcag ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg
    10981 ggtgaatgag gcgctggcag cgcagaagta atttttattc gggcggataa ggcgttttcg
    11041 cctcatccgc cgctctgtac acaatgcctg atgcgacgct gacgcgtcct atcatgccta
    11101 caaaccacat cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta
    11161 ttattgattt ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat
    11221 gagcttagcc cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt
    11281 aactattacg cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt
    11341 tcggcaggct ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt
    11401 gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggca
    11461 gcgggcggta tgttaatcac tgccagcagt cagtcactgg cgatgatgat cctcggtacg
    11521 gcattaaccg gtttattctc agtcgtggca caaattctgg ttccgctggc agcgacgctg
    11581 gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta tgagcggtct gctgttgggg
    11641 atattgctgg cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc
    11701 ttttgggttg cttcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca
    11761 caaatgaaat cagaaaccca cctcaactac ccacagttgt tgggttccgt tttcagcatg
    11821 ttcatcagcg ataagatcct gcgcacccgc gcgttgctgg gctgcatgac ctttgccaac
    11881 ttcagcattc tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc
    11941 gatggtgaca ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg cgctcgtccg
    12001 gcgggcggtt ttgccgacaa gggcaaatcg caccacaact ttcggtctgc ttctgctgtt
    12061 actttcatgg ctggcaatct ggtttggtca cacttccgta ctggcgttga ttatcggcat
    12121 tctggtgctg gacctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg
    12181 aatacatcct gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg
    12241 cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt
    12301 ttgtctggct ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca
    12361 tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag
    12421 ggtgttaaga agcccatcag tctgataagg ttaagatatt cattcagtct atttataata
    12481 ttaacaatcg ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc
    12541 ggcgaccttc atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt
    12601 ggcctttgcg ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt
    12661 tttctcctgc atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc
    12721 cgggagtagt ttgtgggttg ctgcactgag cgtcatggca atggatgttc gccatgtttt
    12781 gtatggcccg tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct
    12841 atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac tggtacgcaa
    12901 taatcgccgc tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg
    12961 ggtatttggt acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc
    13021 cgttgaagct gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc
    13081 tttccagcgc aaacaatctc tttgcgttac cgcagcttta gttggtgccc ttgcaggcgt
    13141 aacgctattt tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc
    13201 gttaatccag gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt
    13261 tactggttgg cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta
    13321 atgcccgccc aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct
    13381 cgatatgcgc tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt
    13441 tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca
    13501 ttatcatccc aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga
    13561 ttatataacc cacaagaatc atttttctaa aacaatacat ttactttatt tgtcactgtc
    13621 gttactatat cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca
    13681 ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc
    13741 ttctgactcg tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc
    13801 tgaaggctca ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg
    13861 aaaaccacag tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg
    13921 cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt aaaagcgata
    13981 acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg
    14041 ttttaccacc gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa
    14101 aagatcagct cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag
    14161 acggtgtggt tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat
    14221 aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc
    14281 tcagccgatg agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa
    14341 ccccgcagca accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct
    14401 tgctctttat aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt
    14461 tcgaagaaac cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt
    14521 ctggcagcgt gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgatgttc
    14581 tggtcactct cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg
    14641 cttccagcgt tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata
    14701 ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc
    14761 tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca
    14821 gtgcccaggc gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc
    14881 tggggactaa actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg
    14941 cctagctggc gctggagcgt acccgtattg tcagtccgat gaccggttat gtttcccgtc
    15001 gtgccgtgca accgggtgcg caaattagcc caacgacgcc gctgatggcg gtcgttccag
    15061 ccaccaatat gtgggtggat gccaacttta aagagacgca gattgccaat atgcgcatcg
    15121 gtcagccggt caccatcacc accgatattt acggcgatga tgtgaaatac accggtaaag
    15181 tggttggtct ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcga
    15241 ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac
    15301 agctggagca atacccgctg cgcattggtt tgtccacgct ggtgagcgtc aataccacta
    15361 accgtgacgg tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg
    15421 cgcgtgaaat cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg
    15481 ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg
    15541 gtcattatga cgattgcgct gtcactggcg acattcatgc aggtgctgaa ctccaccatt
    15601 gctaacgtgg cgatccccac tatcgccggg aatctgggct catcgctcag ccaaggaacg
    15661 tgggtaatca cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg
    15721 gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg
    15781 tcttgggcgt gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag
    15841 gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg
    15901 ccagccaaac gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt
    15961 tgcggcccga tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc
    16021 atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgt
    16081 gaaacccgca ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt
    16141 atcggcagcc tgcagattat gctcgaccgc ggtaaagagc tggactgttt ttcatcacag
    16201 gaaattatca ttcttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag
    16261 ctgaccgacg ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc
    16321 ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg acgctattgt tctgctgccg
    16381 cagttgttgc aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg
    16441 gtagggatta ttccggtgat tctgtcgccg attatcggcc gcttcgcgca taaactggat
    16501 atgcggcggc tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc
    16561 tatacctttg aaccaggtat ggatttttgc gcgtcggcct ggccgcagtt tatccagggg
    16621 tttgcggtgg tctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg
    16681 gaacgactgg cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc
    16741 ggcacgtcga taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg
    16801 actgagtcgg taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg
    16861 cttgggatga cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg
    16921 ctgattattt ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg
    16981 gggctggtgt ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac
    17041 taagtacaac taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt
    17101 tactgactag atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct
    17161 gttgatgcgt acctcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga
    17221 gtgcatctgg taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg
    17281 cactttcagc acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg
    17341 cgtaccaatc agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat
    17401 ctctacacca ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt
    17461 atggatccct ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac
    17521 ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc
    17581 catccgggta tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat
    17641 ttttttaaaa attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg
    17701 catttgttat catcatccct gaattcagag atgaaatttt ggccactccc gagtggcctt
    17761 tttcttttct gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt
    17821 tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc
    17881 agaatttcca gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct
    17941 gttccgccaa taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg
    18001 ggattatcga agcaggcaac cagttcatca cacactttct gatacgcttc gccgccgtta
    18061 atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc
    18121 agtaacggga actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct
    18181 tcaaggatca cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc
    18241 ggtgcatcag ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca
    18301 tctacaccaa tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg
    18361 ccgccacgta acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga
    18421 atcggcgcat acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta
    18481 ccgtctttct caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta
    18541 aggcccgcta cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta
    18601 ttggtatagc cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg
    18661 cactcggttt tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc
    18721 gccggagatg caccaaacag ataaggaatg acccaaccga aacgatagta attgcggata
    18781 acgcggaaat agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac
    18841 ttcgcttgcc agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc
    18901 atcagcgcgc cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg
    18961 ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata
    19021 cttaacggcc acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc
    19081 ataaaggtca gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct
    19141 tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga
    19201 cctgttgttg ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc
    19261 tgtatcccct ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc
    19321 gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga
    19381 ccatatgtgc aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca
    19441 actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa agattactgg cccccaggag
    19501 atgcgcatcc atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat
    19561 aatagcgtga ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat
    19621 gttttacgca atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta
    19681 cccattgttg ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc
    19741 accagcacga cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac
    19801 aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg
    19861 ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt
    19921 gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga
    19981 cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg
    20041 cctcagcgat ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac
    20101 ttttcaccac ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg
    20161 cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa
    20221 ttgcctgagc aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt
    20281 actgaagacc gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat
    20341 ccaggattgt gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct
    20401 cgcaagtcga aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg
    20461 aaggagaaga aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa
    20521 ggaaattttt tggagaa
//
LOCUS       DAJVRL010000074        20478 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVRL010000074 DAJVRL010000000
VERSION     DAJVRL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.074"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            102..1157
                     /gene="phoE"
                     /locus_tag="OQN02_002686"
     CDS             102..1157
                     /gene="phoE"
                     /locus_tag="OQN02_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1643422.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1196..1597)
                     /gene="crl"
                     /locus_tag="OQN02_002687"
     CDS             complement(1196..1597)
                     /gene="crl"
                     /locus_tag="OQN02_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1643423.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1655..2899)
                     /gene="frsA"
                     /locus_tag="OQN02_002688"
     CDS             complement(1655..2899)
                     /gene="frsA"
                     /locus_tag="OQN02_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1643424.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2991..3449)
                     /gene="gpt"
                     /locus_tag="OQN02_002689"
     CDS             complement(2991..3449)
                     /gene="gpt"
                     /locus_tag="OQN02_002689"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1643425.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3711..5168
                     /gene="pepD"
                     /locus_tag="OQN02_002690"
     CDS             3711..5168
                     /gene="pepD"
                     /locus_tag="OQN02_002690"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1643426.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5225..5839)
                     /locus_tag="OQN02_002691"
                     /pseudo
     CDS             complement(5225..5839)
                     /locus_tag="OQN02_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5901..6944)
                     /locus_tag="OQN02_002692"
     CDS             complement(5901..6944)
                     /locus_tag="OQN02_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1643427.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7190..7642)
                     /locus_tag="OQN02_002693"
     CDS             complement(7190..7642)
                     /locus_tag="OQN02_002693"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1643428.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7652..8050)
                     /gene="yafO"
                     /locus_tag="OQN02_002694"
     CDS             complement(7652..8050)
                     /gene="yafO"
                     /locus_tag="OQN02_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCS1643429.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8053..8346)
                     /gene="yafN"
                     /locus_tag="OQN02_002695"
     CDS             complement(8053..8346)
                     /gene="yafN"
                     /locus_tag="OQN02_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCS1643430.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8398..9453)
                     /gene="dinB"
                     /locus_tag="OQN02_002696"
     CDS             complement(8398..9453)
                     /gene="dinB"
                     /locus_tag="OQN02_002696"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS1643431.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9524..10309)
                     /gene="lafU"
                     /locus_tag="OQN02_002697"
     CDS             complement(9524..10309)
                     /gene="lafU"
                     /locus_tag="OQN02_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS1643432.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10281..11993
                     /locus_tag="OQN02_002698"
                     /pseudo
     CDS             10281..11993
                     /locus_tag="OQN02_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12210..12707)
                     /gene="rayT"
                     /locus_tag="OQN02_002699"
     CDS             complement(12210..12707)
                     /gene="rayT"
                     /locus_tag="OQN02_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS1643433.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12883..13404)
                     /locus_tag="OQN02_002700"
                     /pseudo
     CDS             complement(12883..13404)
                     /locus_tag="OQN02_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13788..14528
                     /locus_tag="OQN02_002701"
     CDS             13788..14528
                     /locus_tag="OQN02_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1643434.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14499..15266)
                     /locus_tag="OQN02_002702"
     CDS             complement(14499..15266)
                     /locus_tag="OQN02_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1643435.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(15472..16050)
                     /gene="lpcA"
                     /locus_tag="OQN02_002703"
     CDS             complement(15472..16050)
                     /gene="lpcA"
                     /locus_tag="OQN02_002703"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1643436.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16290..18734
                     /gene="fadE"
                     /locus_tag="OQN02_002704"
                     /pseudo
     CDS             16290..18734
                     /gene="fadE"
                     /locus_tag="OQN02_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(18777..19223)
                     /gene="ivy"
                     /locus_tag="OQN02_002705"
     CDS             complement(18777..19223)
                     /gene="ivy"
                     /locus_tag="OQN02_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1643437.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19404..20174
                     /gene="yafV"
                     /locus_tag="OQN02_002706"
     CDS             19404..20174
                     /gene="yafV"
                     /locus_tag="OQN02_002706"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1643438.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            20284..20478
                     /locus_tag="OQN02_002707"
                     /pseudo
     CDS             20284..20478
                     /locus_tag="OQN02_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         5005 a         5264 c         5269 g         4940 t
ORIGIN      
        1 atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca acggggctct
       61 cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag agcactctgg
      121 cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa atatataata
      181 aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat atgagtgata
      241 acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt gaaactcaaa
      301 ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga aataaagcgg
      361 agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa tataaagatt
      421 tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa gcctggaccg
      481 atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt atgaccaaac
      541 gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc gatggcctga
      601 acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag caaaacggcg
      661 atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc attagtgggg
      721 cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc acaggcaagc
      781 gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat ctggcaactt
      841 tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat aagacacaga
      901 actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg ctgggttatg
      961 tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc aattatatcg
     1021 acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat tataaaatca
     1081 accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc gcggttggga
     1141 tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat ttcactcacg
     1201 ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc agattcagcg
     1261 tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc agtcgttcaa
     1321 ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg aacagaccaa
     1381 actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac cagccccaga
     1441 attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa tcgaaaaaga
     1501 atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta aattttttga
     1561 tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt tgtgatgcaa
     1621 ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt tcgatccagc
     1681 cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat gggatctcta
     1741 ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc gggctgaacg
     1801 gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc agcaatcctt
     1861 gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa gcatcatgca
     1921 tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc tggcacttaa
     1981 aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc agacgcggcg
     2041 attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca aaggccgcga
     2101 cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc tgatgcaaca
     2161 ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc atatcaatag
     2221 tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa tagtccgtct
     2281 gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg cctttcggca
     2341 tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc tgccgcatcg
     2401 tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc tgcgcttgct
     2461 cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac gtagccgcat
     2521 gcagccagtg tcgaccactg agtttcggat cttcttccgt acaggctttt tgttgccaca
     2581 ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca atcacggtat
     2641 cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc tggacgtcga
     2701 ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc atgcgatacc
     2761 agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg ccgtgattaa
     2821 agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac agggtttcgc
     2881 tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac tattgtaacc
     2941 tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga ttagcgaccg
     3001 gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca ggtatcttgc
     3061 gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc gaagatggtg
     3121 acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc ggtatccacc
     3181 aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag cactttaagc
     3241 tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg acgaataccc
     3301 agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc aataatgcct
     3361 ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat ctgcaacatg
     3421 tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt atctacggct
     3481 taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg cgcactaaaa
     3541 accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga cgcgggataa
     3601 agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat tttctgctaa
     3661 ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac gtgtctgaac
     3721 tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt tctattcctc
     3781 acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca aaagagaaag
     3841 gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct gctaccgcag
     3901 gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg ccgcagaaaa
     3961 ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt gatggcgaat
     4021 gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg gcctctgcac
     4081 tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg ctgaccatga
     4141 ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg caggctgata
     4201 ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt gcggggggta
     4261 tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt tttgaaacct
     4321 tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc cacgttggcc
     4381 tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa gaactggacc
     4441 tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt gaagcctttg
     4501 cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg aatacctatc
     4561 aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg ttgctggact
     4621 ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt attcgtctgc
     4681 tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt gtggtcgaaa
     4741 cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt cactgcctga
     4801 tccgttcact gatcgacagc ggtaaagact acgtggtgag catgctggat tcgctgggta
     4861 aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag ccggacgcta
     4921 attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac aagacgccga
     4981 acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg tatccggaaa
     5041 tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat gagcaagttc
     5101 acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa gaaattccgg
     5161 cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc agtttcatcc
     5221 ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg ccacgttcaa
     5281 tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc tattgctgct
     5341 gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc tggctgcgct
     5401 ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc gccgagtcgg
     5461 ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac cggatcgcat
     5521 cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag tttttacgcc
     5581 cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac cagctttcgc
     5641 ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc gtgtcagagt
     5701 agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca gcttctttaa
     5761 ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc tgcgctgagg
     5821 agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta gcaccggacg
     5881 cagccgcgcg acaggaataa ttaatccggc ctgcccagac attgcaccac actttcagcc
     5941 gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca aattacccga
     6001 ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag acgcccttta
     6061 cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa tgtttcctca
     6121 ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat aaccaccaga
     6181 ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc accgattcga
     6241 cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc cttaacctgt
     6301 tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag cgcgtcgtca
     6361 ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa aatagactgc
     6421 cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc cagacctgcc
     6481 agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc gaaatgattg
     6541 ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc tgacggcagg
     6601 ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg cttttcaaac
     6661 ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat gccacagccg
     6721 atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc tgcgccaatc
     6781 gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat gttgggtaaa
     6841 tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc atcagatgcg
     6901 atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca ttccgtaaat
     6961 agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag taacaattaa
     7021 aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc ctggaaaggt
     7081 ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt ttgtggaata
     7141 agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt taatgttgcg
     7201 gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg cgctgctgct
     7261 taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt gcgtccaccg
     7321 taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc ccacggcggg
     7381 tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta ataaagccga
     7441 ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc tccttccagc
     7501 gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc tgactgacgg
     7561 tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca ggatgataat
     7621 ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac gcttctgcca
     7681 tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt tcaggcttca
     7741 gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat accaaatgcg
     7801 cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta ttcacatttg
     7861 ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac gagtcgtcgt
     7921 agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag gaaataaaat
     7981 ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt tttgttttga
     8041 atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat cattaagata
     8101 ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa agcgcgcctt
     8161 tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga atgcgctggc
     8221 acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt gatcaataaa
     8281 gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag cgagaattcg
     8341 atgcatacag tgataccctc ataataatgc acaccagaat atacataata gtatacatca
     8401 taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat gcagccccac
     8461 cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg tggcgattaa
     8521 gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa aatcgtcgaa
     8581 ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg ccagacggcg
     8641 ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt gatgaatatc
     8701 ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt taacgtcgcg
     8761 ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa agcgtttaag
     8821 cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca tcgcttccag
     8881 ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg tttgtaaaaa
     8941 tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca tgtcggaggc
     9001 gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt gcagctcgtt
     9061 gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc aatggacgct
     9121 atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg aggtgtagcg
     9181 cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc gccccggaag
     9241 caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc tacgtacgcc
     9301 aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc ggctgccgcc
     9361 aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg ctgcgaaaaa
     9421 gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac tggtaaagta
     9481 tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc attcgctgcg
     9541 aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta tccccatgct
     9601 gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata atctcaatac
     9661 gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc gccattgcgc
     9721 ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga cgagctgaaa
     9781 gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag gccatcgcat
     9841 cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc gcgagctcca
     9901 ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac atattgcgat
     9961 tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc tccagattcg
    10021 cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg ccaagctcgg
    10081 tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct tttttctccg
    10141 tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg attttattta
    10201 acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg ataatgcttt
    10261 cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc aaagaggccg
    10321 agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc aaacagatgg
    10381 cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa acccggcgta
    10441 aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg aagtttgtac
    10501 gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc ggcgtctgta
    10561 tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac gtgctgatga
    10621 ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc gcggcggcga
    10681 ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc caccagttgc
    10741 tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg gcggtggtgc
    10801 cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt accggctggc
    10861 ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa acgctgaccc
    10921 gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg ctgatcgagc
    10981 ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg cagggcaacc
    11041 cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac ggcgtgctgc
    11101 tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac gctattttca
    11161 tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg gcgctgccct
    11221 ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg tacgggatac
    11281 cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg tatcaggtga
    11341 tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc tatattcctg
    11401 atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg atggcaccgc
    11461 gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa gtgtaccggg
    11521 cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc gtgctggtcg
    11581 ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc ctggcgctgc
    11641 ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat acgctgaata
    11701 atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc ggcaaagtga
    11761 tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc acgatgccac
    11821 aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg ccgccgcagc
    11881 tacgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat gtgctgtcgt
    11941 ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg taagaatccg
    12001 gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac cgttcaggca
    12061 caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg cataactgcc
    12121 ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta ggccggataa
    12181 gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc ggcacttaaa
    12241 tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc gcgatggaac
    12301 gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg atttatataa
    12361 atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc ccaaaaacgc
    12421 ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat ctcccgccag
    12481 cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat atgctccggc
    12541 aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat aatggcgtta
    12601 cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa atttaccgtg
    12661 aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc gactcctttt
    12721 tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat cgggaaagcg
    12781 gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg catcaggcat
    12841 ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa tcatctcttc
    12901 cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg ctaaccggct
    12961 tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc ccagatacac
    13021 ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat ctccccggcg
    13081 caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat tggccgtgcg
    13141 cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac tacaatcgaa
    13201 gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct gctccaggcg
    13261 gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt ggttgttttc
    13321 cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc attctggatg
    13381 ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa aacgcataac
    13441 gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa agacataaac
    13501 cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacgggatt attaattgct
    13561 tatttgcata ttactattga gatttcataa tattaggaaa taaatgatac atgcgctttt
    13621 cggactattg aaaactgccc ctgtgctaaa tttgtaaacc cagcgccgac aggcttgcgt
    13681 aaaactcttt tggtgataat gtgagccttc caaatccaga ctattccgat ttgggatcca
    13741 ttttgccgtc ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg cgtaaaatcg
    13801 cattaattct tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg ctgggcagca
    13861 gtagctcgac cacgccagtg agcaaagagt ataagcagca gttgatggga tcccctgtct
    13921 acatccagat cttcaaggaa gaacgtacgc tcgatcttta cgtcaaaatg ggcgagcaat
    13981 atcaactgct cgacagttat aaaatctgta aatattccgg cggcttaggg ccaaaacagc
    14041 gtcagggcga tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat cagttaaaac
    14101 cagacagccg ttactacaaa gcgattaata ttggattccc caatgcttat gaccgtgcgc
    14161 atggttatga agggaaatac ctgatgatcc acggcgattg tgtatccatc ggctgctacg
    14221 caatgaccaa tcagggtatt gatgagatct tccagttcgt tactggtgcg ctggtgtttg
    14281 gtcagccgag cgtgcaggtg agtatttacc cattccgcat gaccgacgcc aatatgaagc
    14341 gccataaata ttccaacttt aaggacttct gggagcagct gaagccgggc tacgactact
    14401 ttgagcaaac ccgtaagcca ccgaccgttt ctgtggccaa tggccgttac gtggtcagca
    14461 agccgttaag ccacgaagtg gtgcagccac aactggcatc aaactacacg ctccccgagg
    14521 caaaataagt gccattcgcc tggcataatt ttttgccagg tttcattgcc cgtcagcggc
    14581 tgtgtcgcaa tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa gtcgatttcc
    14641 acatcctgat ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat ccagtgtaaa
    14701 ttagtcgagc aatacgccat tacatagcgc ccgtccgaaa gcagcatgtt gaaaacgccc
    14761 ttcgcccgca gttcatccgc cagtgaggcg atatatttaa acactgccgc catgttgccc
    14821 ggcgtgcgcg ggtaacgctg cgttaattta tgcagcagcc agcaaaaggc tttttcgctg
    14881 tcggtttcac cgaccgggcg gaagttgccg gtttccagtg atttgtagcc cgtcagttgt
    14941 ccgttatggg cataagtcca gttacgcccc cataactcgc gggtaaatgg gtgagtattt
    15001 tccagcgcca cctcgccccg attagcctgg cgaatatgag ccaccaccga acaggatttt
    15061 atcgggtagt cctggacaag tttggcgatg ggggaattaa agctgggttg tggatcttta
    15121 aatgtgcgac agcctttacc ttcgtaaaag gtaatgcccc agccatcttt atgtggcccg
    15181 gttccaccac cacgctgtac aagtccggtg aaactaaagc agatatcggt agggacgttg
    15241 gcgctcatcc cgagcaattc gcacatgggt aacctccaca acagcgggag cgcatggacc
    15301 ccggcgaaga ttgatttaag aattttcagg tcggatgcgg cgtaaacgaa ttattcggcc
    15361 tacggttcgg gtacagattt gtaggcatga taagacgcgt cagcatcgca tcaggcatca
    15421 gcgcacaagt gccggatgcg gcgtaaacgc cttatccggc ctacgccaga cttacttaac
    15481 catctctttt tcaattaact ggatcaggat atggatcact ttaatgtgaa tctcctgaat
    15541 gcggtcggca taaccaaagt gcggaacgcg aatttcgata tccgccgtgc cagccatttt
    15601 accgccgtct ttaccggtca gggtgatcac tttcatcccc ttctcacgcg ccgctgcgat
    15661 cgctttgatc acgtttgcag agttaccgga ggtggagatc cccagcagta catcgccttc
    15721 gcgacctacc gcttcaacgt agcgggagaa aatatcattg aaaccgaaat cattaccgac
    15781 gcaggaaata tgactaacgt cagaaatagc aatcgccggg tagcccggac ggttttcacg
    15841 gtagcgaccg gtcaactctt cggcaaagtg catagcgtcg caatgggaac cgccgttgcc
    15901 gcaggaaagc actttgccac cggctttaaa gctgtctgct aacaggaccg ccgcgcgctg
    15961 aatggcgtga atattggcgt catcttttaa aaagttagcc agcgtttccg ccgcttcgtt
    16021 cagttcgtta cgaataagat cctggtacat gaggatatcc ttcagcataa atgtaataga
    16081 caaaatgcag tgtaccggat accgccaaaa gcgagaagta cgggcaggtg ctatgaccag
    16141 gactttttga cctgaagtgc ggataaaaac agcaacaatg tgagctttgt tgtaattata
    16201 ttgtaaacac attgctaaat gtttttacat ccactacaac catatcatca caagtggtca
    16261 gacctcctac aagtaagggg cttttcgtta tgatgatttt gagtattctc gctacggttg
    16321 tcctgctcgg cgcgttgttc tatcaccgcg tgagcttatt tatcagcagt ctgattttgc
    16381 tcgcctagac agccgccctc ggcgttgctg gtctgtggtc ggcgtgggta ctggtgcctc
    16441 tgaccattat cctcgtgcca tttaactttg cgcctatgcg taagtcgatg atttccgcgc
    16501 cggtatttcg cggtttccgt aaggtgatgc cgccgatgtc gcgcactgag aaagaagcga
    16561 ttgatgcggg caccacctgg tgggagggcg acttgttcca gggcaagccg gactggaaaa
    16621 agctgcataa ctatccgcag ccgcgcctga ccgctgaaga gcaagcgttt ctcgacggcc
    16681 cggtagaaga agcctgccgg atggcgaatg atttccagat tactcatgag ctggcggatc
    16741 tgccgccgga gttgtgggcg taccttaaag agcatcgctt cttcgcgatg attatcaaaa
    16801 aagagtacgg cggactggag ttctcagctt atgcccagtc tcgtgtgctg caaaaactct
    16861 ctggcgtgag cgggatcctg gcgattaccg tcggcgtgcc aaactcatta ggcccgggcg
    16921 aactgttgca acattacggc actgacgagc agaaagatca ctatctgccg cgtctggcgc
    16981 gtggtcagga gatcccctgc tttgcactga ccagcccgga agcgggttcc gatgcgggcg
    17041 cgattccgga caccgggatt gtctgcatgg gcgaatggca gggccagcag gtgctgggga
    17101 tgcgtctgac ctggaacaaa cgctacatta cgctggcacc gattgcgacc gtgcttgggc
    17161 tggcgtttaa actctccgac ccggaaaaat tactcggcgg tgcagaagat ttaggcatta
    17221 cctgtgcgct gatcccaacc accacgccgg gcgtggaaat tggtcgtcgc cacttcccgc
    17281 tgaacgtacc gttccagaac ggaccgacgc gcggtaaaga tgtcttcgtg ccgatcgatt
    17341 acatcatcgg cgggccgaaa atggccgggc aaggctggcg gatgctggtg gagtgcctct
    17401 cggtaggccg cggcatcacc ctgccttcca actcaaccgg cggcgtgaaa tcggtagcgc
    17461 tggcaacggg cgcgtattct cacattcgcc gtcagttcaa aatctctatc ggtaagatgg
    17521 aagggattga agagccgctg gcgcgtattg ccgggaacgc ttatgtaatg gatgctgcgg
    17581 catcgctgat tacctacggc attatgctcg gcgaaaaacc tgccgtgctg tcggctatcg
    17641 ttaagtatca ctgtacccac cgcgggcagc agtcgattat tgatgcgatg gatattaccg
    17701 gcggtaaagg cattatgctc gggcaaagca acttcctggc gcgtgcttac cagggcgcac
    17761 cgattgccat caccgttgaa ggggctaaca ttctgacccg cagcatgatg atcttcggac
    17821 aaggagcgat tcgttgccat ccgtacgtgc tggaagagat ggaagcggcg aagaacaatg
    17881 acgtcaacgc gttcgataaa ctgttgttca aacatatcgg tcacgtcggt agcaacaaag
    17941 ttcgcagctt ctggctgggc ctgacgcgcg gtttaaccag cagcacgcca accggcgatg
    18001 ccactaaacg ctactatcag cacctgaacc gcctgagcgc caacctcgcc ctgctttctg
    18061 atgtctcgat ggcactgctg ggcggcagcc tgaaacgtcg tgagcgtatc tcggcacgtc
    18121 tgggggatat tttaagccag ctttacctcg cctctgccgt tctgaagcgt tatgacgacg
    18181 aaggccgtaa tgaagccgat ctgccgctgg tgcactgggg cgtacaggat gcgctgtatc
    18241 aggcagaaca ggcaatggac aatttactgc aaaacttccc gaaccgcgtg gttgccgggc
    18301 tgctgaatgt ggtgatcttc ccgaccggac gtcattatct ggcaccgtct gacaagctgg
    18361 atcataaagt ggcgaagatt ttacaagtgc cgaacgccac tcgttcacgt attggtcgcg
    18421 gtcagtacct gacgccaagc gagcataacc cggttggttt actggaagag gcgttggtgg
    18481 atgtgattgc cgccgacccg attcatcagc ggatctgtaa agagctgggt aaaaatctgc
    18541 catttacccg tctggatgaa ctggcgcaca acgcgctgac gaaggggctg attgataaag
    18601 atgaagccgc tattctggtg aaagcggaag aaagccgtct gcgcagtatt aacgttgatg
    18661 actttgatcc ggaagagctg gcgacgaagc cggtaaagtt gccggagaaa gtgcggaaag
    18721 ttgaagccgc gtaattgaat aacggagccg aaaggctccg tttctttatc cgctaattat
    18781 ttaaaattaa agccatccgg atggttttcc aggctgccgg tcaacgccgc gaacagcacc
    18841 gttttaccat caatcgaaag cgcatcgttc acattcagcc aggtgagttt ctcttgcgac
    18901 gttttctcat caatagccga gaacagcccc gtcatctgat tagatttctc ggaccacatc
    18961 acagcgatac gttgcgagcc acagtcatgc ggtttgcacg cgctcatcac ctgatacgtc
    19021 tcatctccca acgttacggt ttgtgcggga gtataagtac cgcctttcat cacccatgca
    19081 ggtagcttat gcccttgcac catctgatta aacgcagctt tggtggtttc gccctttgca
    19141 aggctgctaa tggttaaatc atcctgcgcc attgcactgg tggcgatgac cagtgcggcg
    19201 actgtcgtta ttgccttaaa catcattcct cccgagctta tcctgcccat gttattaacc
    19261 tcctaaagcc tggcgataac acgataattt gcaaatcaga tatttccaat atcagcattt
    19321 gttatcaatt tattctgcgc taaggcaact gcctgataag gtgttaaagt tataaaaccc
    19381 ttcgcataaa ggagttgtac accgtgccta gtttgaagat tacgcttttg cagcaaccgt
    19441 tggtgtgggt ggatggtcct gccaacctgc gccattttga tcgtcaactg gaaggtatta
    19501 ccgggcgcga tgtgatcgtt ctaccggaga tgtttaccag cggctttgcc atggaagcgg
    19561 cagcttcgtc gcttgcacaa gatgacgtag tgaactggat gacagctaag gcgcagcagt
    19621 gcaatgcgct gattgcaggc agtgttgcat tacaaacgga gtctggttcg gttaaccgct
    19681 ttttgctggt tgagccgggc ggcacggtac atttttatga taagcgtcat ctgttccgca
    19741 tggcagatga gcatctgcat tataaagcgg gcaatgcgcg agtgattgtg gaatggcgcg
    19801 gctggcgtat tttgccgctg gtgtgctacg acttacgttt tccagtgtgg tcgcgcaatc
    19861 tcaacgatta tgacctcgcc ctgtacgtcg ccaactggcc agctccgcgc tctctgcact
    19921 ggcaggcatt gctgacggcc cgcgcgattg agaatcaggc gtatgtggcg ggatgcaatc
    19981 gcgtcggcag cgatggcaac ggctgccatt atcgcggtga cagccgggtg attaatccgc
    20041 aaggcgagat tatcgctacc gccgacgcgc ataaggcaac gcgtattgat gcggagctgt
    20101 cgatggtggc attgcgggaa tatagagaaa agtttccggc atggcgggat gcggatgagt
    20161 ttaggttgtg gtgagtgggg gttgtgttta gggccggggg ctgggctaga ttacgaaagc
    20221 ccctaactta aacacaatat tacgccagaa gtcaccattt ccaaaacaca gcgtcgctgt
    20281 accgtgggtt atctgcccaa ccgcagcgac acgccggtga tcaaaatcag cgccaaatgg
    20341 ctgagaaaag cgggatttga gatcggcacg ggcgttaccg tgaagatctc ggagggatgt
    20401 ttgatcctgc ttgggataac aacgaagtac aggaactgcg cagggaactt tatcaggtta
    20461 aacagccagt taagggga
//
LOCUS       DAJVRL010000075        20287 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVRL010000075 DAJVRL010000000
VERSION     DAJVRL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.075"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(60..818)
                     /gene="aroD"
                     /locus_tag="OQN02_002708"
     CDS             complement(60..818)
                     /gene="aroD"
                     /locus_tag="OQN02_002708"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1643439.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="OQN02_002709"
     CDS             complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="OQN02_002709"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS1643440.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1727..2998)
                     /locus_tag="OQN02_002710"
     CDS             complement(1727..2998)
                     /locus_tag="OQN02_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1643441.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3181..4386)
                     /locus_tag="OQN02_002711"
     CDS             complement(3181..4386)
                     /locus_tag="OQN02_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1643442.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="OQN02_002712"
     CDS             complement(4486..4842)
                     /locus_tag="OQN02_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCS1643443.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="OQN02_002713"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQN02_002713"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="OQN02_002714"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="OQN02_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1643444.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="OQN02_002715"
     CDS             6772..9828
                     /locus_tag="OQN02_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1643445.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="OQN02_002716"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="OQN02_002716"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1643446.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="OQN02_002717"
     CDS             10335..10523
                     /locus_tag="OQN02_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1643447.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="OQN02_002718"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="OQN02_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1643448.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="OQN02_002719"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="OQN02_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1643449.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="OQN02_002720"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="OQN02_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1643450.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="OQN02_002721"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="OQN02_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1643451.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="OQN02_002722"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="OQN02_002722"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1643452.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="OQN02_002723"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="OQN02_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1643453.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="OQN02_002724"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="OQN02_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1643454.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="OQN02_002725"
     CDS             complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="OQN02_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1643455.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="OQN02_002726"
     CDS             complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="OQN02_002726"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1643456.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5314 a         4855 c         5176 g         4942 t
ORIGIN      
        1 ggttaaaaat acttttttta tttcggcggg ggagggtatt tccgccgaaa tattattgct
       61 tatgcctgat gtaaaatagt taataccgtg cgcaaatcat ttaccgagat ttgccctggc
      121 gcagaggctt tttttaccgc accaaaagtt gccgccgagc caaatacttc accaaccaga
      181 cgagaaatta cgccagtttt tgccatcgac atcgtgatga ttggacgatc ggcatactgc
      241 tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat cgctggtact ttgcggcatc
      301 agcgcaatct taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt
      361 tcttcggctt ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
      421 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      481 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      541 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      601 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      661 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      721 tcggatttca cgcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      781 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      841 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      901 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      961 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
     1021 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
     1081 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
     1141 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
     1201 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
     1261 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
     1321 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1381 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1441 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacaccagt
     1501 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1561 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1621 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1681 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1741 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ctgcacatca ttctcaggaa
     1801 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1861 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1921 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1981 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     2041 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     2101 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     2161 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     2221 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     2281 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     2341 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2401 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2461 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2521 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2581 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2641 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2701 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2761 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2821 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2881 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2941 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     3001 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     3061 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     3121 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     3181 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     3241 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3301 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3361 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3421 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3481 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3541 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3601 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3661 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3721 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3781 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3841 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3901 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3961 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4021 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4081 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     4141 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4201 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4261 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4321 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc tggcacccgg tgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaag gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagctac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagcctgg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat tccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacat aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17761 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17821 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17881 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17941 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    18001 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    18061 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    18121 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    18181 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18241 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18301 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18361 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18421 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18481 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18541 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18601 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18661 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18721 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18781 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18841 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18901 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18961 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    19021 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    19081 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    19141 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    19201 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19261 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19321 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19381 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19441 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19501 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19561 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19621 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19681 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19741 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19801 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19861 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19921 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19981 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    20041 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    20101 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
    20161 ataagtgaaa gttcatccgg tgtataaata tatcatcgct caattgctac agttaaaata
    20221 atttatcgcg ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata
    20281 tgctgat
//
LOCUS       DAJVRL010000076        19808 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVRL010000076 DAJVRL010000000
VERSION     DAJVRL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.076"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     misc_feature    <1..99
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            121..1281
                     /gene="pheA"
                     /locus_tag="OQN02_002727"
     CDS             121..1281
                     /gene="pheA"
                     /locus_tag="OQN02_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1643457.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1324..2445)
                     /gene="tyrA"
                     /locus_tag="OQN02_002728"
     CDS             complement(1324..2445)
                     /gene="tyrA"
                     /locus_tag="OQN02_002728"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1643458.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2456..3526)
                     /gene="aroF"
                     /locus_tag="OQN02_002729"
     CDS             complement(2456..3526)
                     /gene="aroF"
                     /locus_tag="OQN02_002729"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1643459.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            3736..4101
                     /locus_tag="OQN02_002730"
     CDS             3736..4101
                     /locus_tag="OQN02_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1643460.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4251..4769
                     /locus_tag="OQN02_002731"
     CDS             4251..4769
                     /locus_tag="OQN02_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1643461.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            4759..5984
                     /gene="dgcN"
                     /locus_tag="OQN02_002732"
                     /pseudo
     CDS             4759..5984
                     /gene="dgcN"
                     /locus_tag="OQN02_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6000..6482
                     /locus_tag="OQN02_002733"
     CDS             6000..6482
                     /locus_tag="OQN02_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1643462.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(6559..6906)
                     /gene="rplS"
                     /locus_tag="OQN02_002734"
     CDS             complement(6559..6906)
                     /gene="rplS"
                     /locus_tag="OQN02_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1643463.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(6948..7715)
                     /gene="trmD"
                     /locus_tag="OQN02_002735"
     CDS             complement(6948..7715)
                     /gene="trmD"
                     /locus_tag="OQN02_002735"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1643464.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(7746..8294)
                     /gene="rimM"
                     /locus_tag="OQN02_002736"
     CDS             complement(7746..8294)
                     /gene="rimM"
                     /locus_tag="OQN02_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1643465.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8313..8561)
                     /gene="rpsP"
                     /locus_tag="OQN02_002737"
     CDS             complement(8313..8561)
                     /gene="rpsP"
                     /locus_tag="OQN02_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1643466.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(8698..10059)
                     /gene="ffh"
                     /locus_tag="OQN02_002738"
     CDS             complement(8698..10059)
                     /gene="ffh"
                     /locus_tag="OQN02_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1643467.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10226..11017
                     /locus_tag="OQN02_002739"
     CDS             10226..11017
                     /locus_tag="OQN02_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1643468.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11038..12324
                     /locus_tag="OQN02_002740"
     CDS             11038..12324
                     /locus_tag="OQN02_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1643469.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12379..12972)
                     /gene="grpE"
                     /locus_tag="OQN02_002741"
     CDS             complement(12379..12972)
                     /gene="grpE"
                     /locus_tag="OQN02_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1643470.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13095..13973
                     /gene="nadK"
                     /locus_tag="OQN02_002742"
     CDS             13095..13973
                     /gene="nadK"
                     /locus_tag="OQN02_002742"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1643471.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14059..15720
                     /gene="recN"
                     /locus_tag="OQN02_002743"
     CDS             14059..15720
                     /gene="recN"
                     /locus_tag="OQN02_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1643472.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            15869..16210
                     /gene="bamE"
                     /locus_tag="OQN02_002744"
     CDS             15869..16210
                     /gene="bamE"
                     /locus_tag="OQN02_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1643473.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16272..16562)
                     /locus_tag="OQN02_002745"
     CDS             complement(16272..16562)
                     /locus_tag="OQN02_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1643474.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(16552..16989)
                     /gene="ratA"
                     /locus_tag="OQN02_002746"
     CDS             complement(16552..16989)
                     /gene="ratA"
                     /locus_tag="OQN02_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1643475.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17160..17642
                     /gene="smpB"
                     /locus_tag="OQN02_002747"
     CDS             17160..17642
                     /gene="smpB"
                     /locus_tag="OQN02_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1643476.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            17857..18219
                     /gene="ssrA"
                     /locus_tag="OQN02_002748"
     tmRNA           17857..18219
                     /gene="ssrA"
                     /locus_tag="OQN02_002748"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18424..19665
                     /locus_tag="OQN02_002749"
     CDS             18424..19665
                     /locus_tag="OQN02_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1643477.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5069 a         5197 c         4671 g         4871 t
ORIGIN      
        1 attctttttt accttcccct gaatgggagg cgtttcgtcg tgtgaaacag aatgcgaaga
       61 cgaacaataa ggcctcccaa atcggggggc cttttttatt gataacaaaa aggcaacact
      121 atgacatcgg aaaacccgtt actggcgctg cgagagaaaa tcagcgcgct ggatgaaaaa
      181 ttattagcgt tactggcaga acggcgcgaa ctggccgtcg aggtgggaaa agccaaactg
      241 ctctcgcatc gcccggtacg tgatattgat cgtgaacgcg atttgctgga aagattaatt
      301 acgctcggta aagcgcacca tctggacgcc cattacatta ctcgcctgtt ccagctcatc
      361 attgaagatt ccgtattaac tcagcaggct ttgctccaac aacatctcaa taaaattaat
      421 ccgcactcag cacgcatcgc ttttctcggc cccaaaggtt cttattccca tcttgcggcg
      481 cgccagtatg ctgcccgtca ctttgagcaa ttcattgaaa gtggctgcgc caaatttgcc
      541 gatattttta atcaggtgga aaccggccag gccgactatg ccgtcgtacc gattgaaaat
      601 accagctccg gtgccataaa cgacgtttac gatctgctgc aacataccag cttgtcgatt
      661 gttggcgaga tgacgttaac tatcgaccat tgtttgttgg tctccggcac tactgattta
      721 tccaccatca atacggtcta cagccatccg cagccattcc agcaatgcag caaattcctt
      781 aatcgttatc cgcactggaa gattgaatat accgaaagta cgtctgcggc aatggaaaag
      841 gttgcacagg caaaatcacc gcatgttgct gcgttgggaa gcgaagctgg cggcactttg
      901 tacggtttgc aggtactgga gcgtattgaa gcaaatcagc gacaaaactt cacccgattt
      961 gtggtgttgg cgcgtaaagc cattaacgtg tctgatcagg ttccggcgaa aaccacgttg
     1021 ttaatggcga ccgggcaaca agccggtgcg ttggttgaag cgttgctggt actgcgcaac
     1081 cacaatctga ttatgacccg tctagaatca cgcccgattc acggtaatcc atgggaagag
     1141 atgttctatc tggatattca ggctaatctt gaatcagcgg aaatgcaaaa agcattgaaa
     1201 gagttagggg aaatcacccg ttcaatgaag gtattgggct gttacccaag tgagaacgta
     1261 gtgcctgttg atccaacctg atgaaaaggt gccggatgat gtgaatcatc cggcactgga
     1321 ttattactgg cgattgtcat tcgcctgacg caataacacg cggctttcac tctgaaaacg
     1381 ctgtgcgtaa tcgccgaacc agtgctccac cttgcggaaa ctgtcaataa acgcctgctt
     1441 atcgccctgc tccagcaact caatcgcctc gccgaaacgc ttatagtaac gtttgattaa
     1501 cgccagatta cgctctgacg acataatgat gtcggcataa agctgcggat cctgagcaaa
     1561 cagtcgcccg accatcgcca gctcaaggcg gtaaatcggc gaagagagcg ccagaagttg
     1621 ctcaagctga acattttctt ctgccaggtg cagcccgtaa gcaaaagtag caaagtggcg
     1681 cagagcctga ataaacgcca tattctgatc gtgctcgaca gcgctaatac gatgcaaccg
     1741 agcgccccag acatgaattt gctccagaaa ccattggtat gcttccggct tacgtccatc
     1801 acaccagacc acaacttgct ttgccaggct accgctgtcc gggccgaaca tcgggtgtaa
     1861 ccccagtacc gggccatcgt gcgccgccag catggcctgt aatggtccat ttttcactga
     1921 tgccagatca accagaatac aatctttcgg taaaggcggt aatttgccga taacttgctc
     1981 agtaacgtgg attggcacac taacaatcac cattccggca tcggcaacaa tatcagccgc
     2041 tcgatcccag tcatgttgct ccagaatccg cacctgataa cccgatagtg tcagcatctt
     2101 ctcgaacagg cgtcccatct gaccgccacc gccgacgata accaccggac gcagtgacgg
     2161 acaaagtgtt ttaaatcctt tgtcgttttc actggagtaa gattcacgca tcacccgacg
     2221 caaaacatcc tcaatcagat ctggcggtac acccagagct tccgcctctg cacgacgcga
     2281 ggccaacata gatgcctcgc gctccggaac ataaataggc agtccaaagc ggcttttcac
     2341 cttgcccact tcagcaacca gttccagacg cttcgctaat aaattcagca gcgctttatc
     2401 gacttcatca atttgatcgc gtaatgcggt caattcagca accataataa acctcttaag
     2461 ccacgcgagc cgtcagctgc ccgttcagat cctgatgaat ttcacgcagc aaggcatcgg
     2521 tcatttccca gctaatgcag gcatcggtta cggatacacc gtatttcatt tcactgcgcg
     2581 gttgctcaga agactgattg ccctcgtgga tattactttc gatcatcaga ccaataattg
     2641 agcgattacc atctttgatt tgagcaacca cagattctgc caccgcaggc tgacggcgat
     2701 aatctttatt ggaattaccg tggctgcaat ctaccatcag agacgggcgc agtcccgcct
     2761 gttccatctc tttttcacat tgcgcaacat ccgcagggct atagttcggc gctttaccac
     2821 cgcgcaggat cacatgcccg tccggattcc cctgagtttg tagcaacgca acctgccctg
     2881 cctggttaat gccaacaaaa cagtgcggct gggcggcggc gcgcatagcg ttaattgctg
     2941 ttgccagact gccgtcggtg ccgtttttaa aaccaaccgg catggaaagc ccggaggcca
     3001 tttcacggtg agtttgcgat tccgttgtac gagcaccaat tgctgaccag ctaaacagat
     3061 cgcccaggta ttgcgggcta ttcggatcta acgcttccgt cgccagtggc agtcccatat
     3121 tcaccagctc aagcaacaat ttacgcgcga tctgcagccc ggcttctaca tcaaaagagc
     3181 catccatatg gggatcgtta attaaccctt tccagccgac agtggtacgg ggtttttcaa
     3241 aatagacgcg cattaccaga tagaggctat cgctgacctc tgcggcaaga gctttaaatc
     3301 gacgagcata ttccagagca gtttccggat catgaatgga acaaggacca catactacca
     3361 gcagacgagg atcgcgcccg gcgataatat ctgaaatggt tttacgcgag tcagcaatct
     3421 gggcttcttg ttgcaggctc aatggaaaag cggccttcag ttgttccgga gtcattaaaa
     3481 cctgttcgtc ggtaatatgt acgttattca gcgcgtcttt atgcatgatg gcgatcctgt
     3541 ttatgctcgt ttgcgatagt tgctcctcag cgaggatgac gtaacgataa cacataaagt
     3601 aaagttttca atccatattt cgtacatttt tatttacaca gacaatttag tcgcgctttc
     3661 aacccttgct tctgtataga taaatttaca ttccctttaa aaacaatccg ctatgctttg
     3721 aaaaaggaga aagaaatgat gaaaaagttt atcgccccct tgttggcttt actggttagc
     3781 ggatgtcaga ttgatcctta tactcacgcg ccaaccttga ccagcaccga ctggtatgat
     3841 gtcggtatgg aagatgcgat atcgggcagc gccataaaag atgacgatgc atttagcgat
     3901 tcacaggcgg atcgcggtct ataccttaaa ggatatgccg aagggcaaaa gaaaacttgc
     3961 cagaccgatt ttacctatgc ccggggactt tccggtaaaa gctttcctgc cagctgcaac
     4021 aatgttgaaa atgccagcca actgcatgaa gtatggcaaa aaagggctga tgaaaatgcc
     4081 agcacaatac gattaaatta attaaaaccc cacaaataaa ttaaacataa gattttactc
     4141 attgtgtttt atttctcaca ttgatgacgg tcgcatgaaa aaatgatatt ttaatgagca
     4201 tcctgaaaaa atatcgatgc atttcgagcg aagatggtgg ggatccctga atgcgttttt
     4261 ctcaccgact gttcttactt ctgattctcc tgctgacggg agcaccaata ctcgctcaag
     4321 agccttctga cgttgcgaaa aacgtgcgta tgatggtttc cggtattgtc agttacaccc
     4381 gctggcctgc gctatcaggg ccaccaaagt tatgtatctt ttcatcttca cgttttagta
     4441 ccgccctaca agagaatgcc gcgacatctt tgccttatct gcctgtcatc attcacactc
     4501 aacaagaggc gatgatttca ggctgcaatg gtttttattt tggcaacgag tcaccgacat
     4561 tccagatgga attaaccgaa caatatccgt caaaggcgtt attattaatt gccgaacaaa
     4621 ataccgagtg cattattggt agcgcctttt gtctgatcat ccacaataat gacgtcagat
     4681 ttgccgtaaa cctggatgcc tcatcgcgta gcggtgtaaa ggtcaacccg gatgtcttaa
     4741 tgctcgcacg gaagaaaaat gatggataac gataattctc ttaataagcg ccccacgttt
     4801 aaaagagcat tacgcaacat cagtatgacc agcatattta tcactatgat gctgatctgg
     4861 ttgctgcttt ccgtgacctc ggtgctgacc ctgaaacagt acgcgcaaaa aaacctggca
     4921 ctgacagcag caacaatgac ttacagtctg gaagcagctg tcgtttttgc cgatggccct
     4981 gcagcaactg aaacactggc agcgctgggc cagcaagggc attttcaact gcagaagtac
     5041 gtgataagca gcaaaatatt ctggcatcct ggcattaccc ccgtaaggat ccaggcgata
     5101 ctttcagcaa tttcataagc cactggctct tccctgcccc catcattcag ccgattcgtc
     5161 acaatggtga aaccattggc gaagtacgct taaccgctcg cgacagttca atcagccatt
     5221 ttatctggtt ttcgctcgcc gtactgaccg gttgtattct gctggcatca ggcatcgcaa
     5281 ttaccctcac ccgccatttg cacaatggcc tggtggaagc actgaaaaat atcaccgatg
     5341 tcgtacatga tgtgcgttcc aaccgcaatt tttcccgacg agtttcggaa gaacgtatcg
     5401 ctgagtttca ccgcttcgct ctcgacttca acagtctgct ggatgaaatg gaagagtggc
     5461 agcttcgttt acaggccaaa aatgcgcagc ttctgcgtac cgcgctacat gacccattaa
     5521 ccgggctggc taaccgcgca gcgtttcgta gcggcatcaa tacgttgatg aacaattccg
     5581 atgcccgaaa aacgtcggcg ttactatttc ttgatggcga taatttcaaa tatatcaatg
     5641 atacctgggg tcatgcgacg ggcgataggg tcttgattga aatcgcaaaa cggttagctg
     5701 aatttggcgg gctgcgacat aaagcatacc gcctgggcgg cgatgaattc gctatggtgc
     5761 tctatgatgt acagtcggaa tctgaagtgc agcagatatg ctcagcactg acacaaatct
     5821 ttaatctccc gtttgatctt cataatggcc atcagaccac catgacatta agcattggtt
     5881 acgcgatgac cattgagcac gcctctgcgg aaaaattaca agagcttgcc gatcacaata
     5941 tgtatcaggc caaacaccag cgtgccgaaa agctggtgag ataacaagga tatatcgata
     6001 tgataaagca cctggtagca cccctgattt tcacctcact aatactgact ggctgccagt
     6061 cccctcaggg aaagtttact cctgagcaag tcgccgctat gcaatcttat ggatttactg
     6121 aatccgccgg cgactggtcg ctgggcttat cagatgccat tctgttcgca aaaaatgact
     6181 acaaattgct cccggaaagc cagcaacaga tccaaaccat ggcagccaaa ttggcctcga
     6241 cagggctaac acatgcccgt atggatggac acaccgataa ctatggtgaa gacagttaca
     6301 acgaaggctt atcattgaaa cgggcgaatg tcgtggccga tgcatgggct attggtggac
     6361 aaattccacg cagcaatctc accacacagg gtttaggaaa aaaatatccc atagccagta
     6421 acaagaccgc ccagggccgc gccgagaacc gccgcgtcgc agtggtgatt acaacccctt
     6481 aaaacacgag caaacagaca aaaaaagggc cagccaattg gccagccctt cttaacagga
     6541 tgtcgcttaa gcgaaatctt agttaagacg ctctttgata cgagcagcct taccagtacg
     6601 ctcacgcagg tagtacagtt tagctttacg aacagcacca cgacgtttga cagaaatgct
     6661 gtcaactacc ggagagtgag tctggaagac acgctcaacg ccttcgccgt tggaaatttt
     6721 acgaacagtg aatgcagagt gcagaccgcg gttacgaata gcgataacca cgccctcgaa
     6781 tgcctgcaga cgttttttgg aaccttcaac aacccatact ttcacttcca cggtatcacc
     6841 cggacggaag gaaggtacgt cctgcttcat ctgctcttgt tcaagttgct taataatgtt
     6901 gctcataatt taatctctta tcctgggtaa actgatctct cgggggctta cgccatccca
     6961 tcatgtttat gttgctgttg tgcgtgttcc gttttgaact ccgccagcaa ccttgcttgc
     7021 tcttcagtca gagccaggtt ttccagaagt tcaggtcttc taagccaggt acggcccagc
     7081 gactgtttca aacgccagcg acgtatctcg gcatggttgc ccgacagtaa cactggcgga
     7141 acttccatcc cttctaacac ctcaggccgc gtatagtgcg ggcaatccag caatccttca
     7201 gcaaaggaat cttccgttgc cgaggcttca tgtcccagta ctcccggaat aaaccgggaa
     7261 acggagtcaa tcagcgtcat tgctggtaac tcaccaccac tgagaacgta atcgccgatt
     7321 gaccattctt cgtcaatttc ggtttggatc acgcgctcat ctataccttc gtagcgaccg
     7381 cacaccagaa tcaatttttg attcgttgcc agttcgctga cgcccgcttg atcaagcttg
     7441 cgtccctgtg gtgacagata aatcaccttt gcgccttcac ccgccgcggc ttttgctgca
     7501 tgaatggcgt cccgcaaggg ttgcaccatc attaacatcc ccggtccgcc gccgtaagga
     7561 cgatcgtcca cggtacggtg ccggtcatgc gtgaagtcgc gaggactcca actctggatg
     7621 ctcagcaggc catttttaac tgcccggcca gttaccccgt aatcggtaat tgcgcggaac
     7681 atttcaggaa acaggctaat tatgccaatc cacatagcgc cgccttttac cgtttatccg
     7741 gtggtttaaa aaccaggatc ccaatctact tcgattgaac gagtagtgag atcgactttc
     7801 ttgataacct gcccatcgag gaacggtacg agacgttcct tgataccaaa cgcatctttc
     7861 aggtttgcct taatgacgag aacgtcatta gatccggttt ccatcatatc aacgacttta
     7921 ccgagatcgt agccttcagt ggttactacc tggcagccca tcaggtcttt ccagtagtag
     7981 tccccctctt caagctgagg cagctgcgat gaatccacga caatttcaca attcgtcagc
     8041 aggttcgccg catcacgatc gtcaacgcct ttcagcttga tgatcatgtc ctgattgtgg
     8101 tgcttccagc tttccagctg gacttgctgc cactgacccg ccttctggat aaaccagggc
     8161 tgatagtcaa aaatgctttc ggcgtcttcg gtggaagaaa acactctgag ccacccacga
     8221 ataccgtaag acgaacccat ttttcccaaa acgatgggat caacaggtgc ttgcgcggtg
     8281 agttgtttgc tcatcatgac caccgtgaca gattaagctg ctttgtttac ttctttgatc
     8341 agcgcagcaa cgcgatcaga aatagttgcg ccctggccaa cccagtgagc gatgcgatcc
     8401 agatccaggc gagtgccttc ttctttttcg ctagcgattg ggttgaagaa accaacgcgc
     8461 tcgatgaagc gaccgttgcg tgcattacgg ctgtcagcga caacaacctg gtagaacgga
     8521 cgctttttag cgccgtgacg tgctaaacga atagttacca taacatcctc ttgtgtgaat
     8581 aaaacaaccg gaccccatcg aggaacggag tccggtgtca tattaaaagc ccgaaaattt
     8641 tactgatttc tggagaaaat gcaatcagca gttaataact ctgtcgcata aggtcattta
     8701 gcgaccagga aatcccggcg gcatcattcc cttcatgctt ctcatcatct tcgccattcc
     8761 gcctttcttc attttcttca tcatgcgctg catgtcgtcg aactgcttca gaaggcggtt
     8821 aacgtcctgt acctgcatcc cgcaaccggc ggcgatacga cgcttacgcg aacctttgat
     8881 gatttctggc ttagcgcgct ctttcatcgt cattgagttg atgatggctt ccatacgcac
     8941 cagcacttta tcgtccatct gcgacttgac gttatccggg atctgcccca tgcccggcag
     9001 tttgcccatc aagctggcca tgccgcccat atttttcatc tggcgcagtt gctcaagaaa
     9061 gtcgttgaga tcgaagccgt cgcctttttt tagcttgctg gctaatttct ctgcctgcgc
     9121 gcggtcaact ttgctttcga tatcttcgat cagcgacagt acgtcgccca tgccgagaat
     9181 acgcgacgcg atgcggtccg gatggaacgg ctccagcgcc tcagttttct cgccaacacc
     9241 gaggaacttg atcggtttgc cagtgatgtg acgaatagag agcgccgcac cgccgcgggc
     9301 atcgccgtcc actttggtca acactacgcc ggtaagcggt aacgcttcat tgaatgcttt
     9361 tgccgtattg gccgcatcct gaccggtcat ggcgtcaacc acaaacaggg tttcaaccgg
     9421 gttaatcgac gcatggactt gtttgatctc gtccatcatc gcttcgtcaa cgtgcagacg
     9481 accagcggta tccaccagca gcacgtcgta gaatttcagt ttggcttctt tcagcgccgc
     9541 gttaacaata tcaaccggct tctgaccaac atcagaaggg aagaaatcaa cgcccacctg
     9601 ctctgccagc gtctcaagct gtttgattgc cgccgggcga tagacgtcag cagaaaccac
     9661 cagcactttc ttcttgtgct tctcgcgcag gaaattaccg agtttaccaa cgctggttgt
     9721 tttaccggca ccttgcaggc ctgccatcag cacgaccgca ggcggttgcg cagccagatt
     9781 cagggtctgg ttctcttcgc ccatcgccgc aaccagttcg ttacggacga ttttgacgaa
     9841 ctcctgcccc ggcgtcaggc tcttattaac ttcatgacca accgctttct cttttacgcg
     9901 attgataaac tcacgcacta ccggcagagc gacgtccgcc tccagcagcg ccatgcgcac
     9961 ttcgcgcagc gtatctttta cgttgtcttc agtgaggcgt ccacggccac tgatattgcg
    10021 cagcgtgcgc gacaaacgat cggttaaatt atcaaacatt gtctctcgcc tggggtggaa
    10081 acggttggcc gcaatcgcga cacatcatca gtattttgcc gcagtataac atgaaggcgt
    10141 ctttgttgtt atgcaacggt tggagcagcg ttcacctgac gctatactgc ttctctttct
    10201 tattgctcaa actgtcgaca ttactatgcc cgtttttgct ctgctcgcgc ttgtcgccta
    10261 ctccgtcagt cttgcgctga ttgttcccgg tctgctgcaa aaaaacggcg gctggcggcg
    10321 catggctatt atttctgcgg tcattgcgct ggtctgccac gcaatcgctc tggaagcccg
    10381 catcctgccc gacggtgata gcggacaaaa cctcagcctg ctgaacgttg gttcattggt
    10441 cagtttggtg atctgtacgg taatgaccat tgtggcttct cgcaatcgtg gctggttgct
    10501 gctacccatt gtctatgcct ttgcgcttat caacctggcg ctggcgacct tcatgcccaa
    10561 tgaatacatc acccatctgg aagcaacgcc tgggatgctg atgcacattg gcttatcgct
    10621 cttttcctat gccacgctaa ttatcgccgc cctgtacgcg ctgcaactgg cgtggattga
    10681 ttaccaactg aagaacaaga agctggcatt taaccaggaa atgccgccat tgatgagtat
    10741 cgagcgtaaa atgttccaca tcacgcagat tggcgtggtg ctgctaacgc tcacgctttg
    10801 cactggcctg ttctacatgc acaacctatt tagcatggaa aatatcgaca aggctgtgct
    10861 ctctatcgtg gcgtggtttg tctatattgt gctgctgtgg ggacattacc atgaaggatg
    10921 gcgtggacgc cgcgtcgtct ggtttaacgt tgcgggcgcg gtaattctga cactggccta
    10981 cttcggcagc cgaattgtcc agcagttaat cagctaaacc cagaaaagga gtttcccctg
    11041 gaacacattt ctactactac gttgatcatt attctgatca tcatggtggt catttcagcc
    11101 tatttttccg ggtccgaaac cggaatgatg accctcaacc gctatcgtct tcgacatatg
    11161 gcgaaacagg gtaatcgctc ggccaaacgc gtcgaaaaat tgctgcgtaa gccagaccgc
    11221 ctgataagcc tggtgttaat cggcaataac ctggtcaata ttcttgcctc cgcgctcggc
    11281 actattgtcg ggatgcgttt gtacggcgat gcgggcgtgg caattgcgac tggtgtgctg
    11341 acttttgtcg tactggtttt tgctgaggta ttgccgaaaa ccattgccgc gctgtacccg
    11401 gaaaaagtcg cttatccgag tagttttctg ctggctccgc tgcaaatttt gatgatgccg
    11461 ctggtctggt tgctgaatgc tatcacccgt atgctgatgc gcatgatggg tatcaaaacc
    11521 gatatcgtgg ttagcggctc tttgagcaaa gaagagttgc gcactatcgt gcatgaatcg
    11581 cgctcacaaa tttcccgtcg caatcaggat atgctgctgt cggtgctcga tctggaaaaa
    11641 atgaccgttg atgacatcat ggtaccgcgc agtgaaatta tcggtattga tatcaacgat
    11701 gactggaaat ctattctgcg ccaactctcc cactcacctc acgggcgcat cgtgctttac
    11761 cgtgattcgc tggacgacgc catcagtatg ctgcgagtac gtgaagcctg gcggttgatg
    11821 tcggagaaaa aagagttcac caaagaaacc atgctgcgcg ccgcggacga gatctatttt
    11881 gtgcctgaag gtacgccgct cagcacgcag ttggtaaagt ttcagcgcaa caaaaagaaa
    11941 gtcggcctgg tcgtcaacga gtatggagac attcaggggc tggtgacggt tgaagatatt
    12001 ctggaagaga ttgtcggcga tttcactacg tcgatgtcgc caacacttgc cgaagaggtc
    12061 acgccgcaaa acgacggttc ggtgattatc gatggcaccg ccaacgtgcg ggaaatcaac
    12121 aaagccttta actggcatct accggaagat gatgcccgca cggttaatgg cgtcattctt
    12181 gaggcactgg aagagatccc tgtcgcaggc acccgcgtgc gtattggcaa gtacgatatc
    12241 gatattctcg acgtacagga caatatgatt aagcaggtaa aagtttttcc tgtgaaaccg
    12301 ctgcgcgaga gtgtggcgga gtaacgaaaa cggccctgca ttcgcatgca gggccgtgaa
    12361 ttattacgaa aacagaaatt acgcttttgc tttcgctaca gttaccatcg ccgcacgaat
    12421 cgtacgacca ttcagcgtat aacccttctg cataatgccc agtacgttac ctggcgcaac
    12481 gtcatcagat tccaccattg cgatagcctg atgcacattc gggtccagcg gcacgttagt
    12541 ttcggcgatc acttcaacac caaacttacg cacaacatcc agcatcgact tcagcgtcag
    12601 ctcaatgcct tcaaccatcg cagacatatc cgggttagct ttatcagcca cttccagcgc
    12661 acgatccagg ctatcaatca ccggcagcaa ttcgttgatg aatttctcca gcgcgaattt
    12721 gtgggctttt tcaatatcca gttcagtacg acgacgcagg ttttccattt cggcttttac
    12781 gcgcaaaata ccgtcacgtt cacgggtctg ggcttcagcc agctgagctt cgagattcgc
    12841 aattttttca tcgcgcggat ccacctgctc agcagaagct tctggctcaa ctgcctcaat
    12901 ctcttcgtgc tgatccatga taatttcttc cggggcttgc ccctcaggcg ttttctgttc
    12961 tttactactc atgaatttct ccgcgttttt ttcgcattca tctcgctaac ttcgcttatt
    13021 atggggatca gtttcagggt ttcaagggaa gcactcacat tgtcatcaat cttcgcaaca
    13081 aggacctcgg aaaaatgaat aatcatttca agtgtattgg cattgtggga cacccacggc
    13141 accctactgc actgacaaca catgaaatgc tctaccgctg gctgtgcaca aaaggttacg
    13201 aggtcatcgt tgagcaacaa atcgctcacg aactgcaact gaagaatgtg aaaactggca
    13261 cgctcgcgga gattgggcaa ctggctgatc tcgcggtagt cgttggtggc gacggtaata
    13321 tgctgggcgc ggcgcgcacg ctcgcccgtt acgatattaa agttattgga atcaaccgtg
    13381 gcaacctggg tttcctgact gaccttgacc ccgataacgc ccagcaacaa ttagccgatg
    13441 tgctggaagg tcactacatc agcgagaaac gttttttgct ggaagcgcaa gtctgtcagc
    13501 aagattgcca aaaacgcatc agcaccgcga ttaacgaagt ggtacttcat cctggcaaag
    13561 tggcgcatat gatagagttc gaagtgtata tcgacgagat ctttgcgttt tctcagcgat
    13621 ctgatgggct aattatttcg acgccaacag gctccaccgc ctattccctc tctgcgggcg
    13681 gtcctattct gaccccctct ctggatgcga ttaccctggt gcccatgttc ccgcatacgt
    13741 tgtcagcacg accactggtc ataaacagca gcagcacgat ccgtctgcgt ttttcgcatc
    13801 gccgtaacga cctggaaatc agttgcgaca gccagatagc actgccgatt caggaaggtg
    13861 aagatgtcct gattcgtcgc tgtgattacc atctgaatct gattcatccg aaagattaca
    13921 gttatttcaa tacattaagc accaagctcg gctggtcaaa aaaattattc taattttacg
    13981 ccagcctctt tactgtatat aaaaccagtt tatactgtac acaataacag taatggtttt
    14041 tcatacagga aaacgactat gttggcacaa ctgaccatca gcaactttgc tatcgttcgt
    14101 gagcttgaga ttgattttca tagcggcatg accgtaataa ctggcgagac tggtgcgggt
    14161 aaatctattg caatagatgc cctcggtctt tgtctcggtg gtcgcgctga agccgacatg
    14221 gtgcgtaccg gcgctgctcg cgctgacctg tgcgcccgtt tttctctgaa agatacgcca
    14281 gcggccctgc gctggctgga agaaaaccag cttgaagacg ggcatgaatg tttgcttcgt
    14341 cgcgtgatca gcagtgatgg tcgctcccgt ggtttcatca acggtacagc tgttcctctg
    14401 tcacaactgc gcgaactggg tcagttgctg attcagatcc atggtcagca cgctcatcaa
    14461 ttactcacca aacctgagca ccaaaaattc ctgcttgatg gctatgccaa tgaaacctct
    14521 ctacttcagg aaatgaccgc acgttatcag ttgtggcatc aaagctgccg tgacctcgcg
    14581 catcatcaac agttaagtca ggaacgcgcc gcccgtgcgg aactgctgca ataccaatta
    14641 aaagaactta acgaatttaa tccgcagccc ggagagtttg agcaaatcga cgaagagtac
    14701 aaacgtctgg cgaacagcgg tcaattgctg accaccagcc agaatgcatt ggcattaatg
    14761 gccgacggtg aagacgcaaa cctgcaaagt cagctttaca cggctaaaca actggtgagc
    14821 gaattgattg gcatggacag caaactgtcc ggcgtacttg atatgctgga agaagctacc
    14881 atccagattg ctgaagccag cgacgaactg cgccactact gcgatcgtct ggatctcgat
    14941 cccaaccgac tgtttgaact tgaacagcgc atctcaaaac agatttcgct ggcacgtaaa
    15001 catcacgtca gccctgaggc attaccacag tattaccagt cgctactgga agaacagcag
    15061 caactggacg atcaggccga ctcacaagaa acgctcgcgc tggcggtaac gaaacatcat
    15121 cagcaggcac tggaaaccgc gcgcgcatta caccaacaac gccagcaata tgcagaagaa
    15181 cttgcacagc tgatcaccga cagtatgcat gcgctctcaa tgccgcatgg gcagtttacg
    15241 attgatgtca aatatgacga gcatcacctg ggcgctgacg gtgccgatcg tattgagttt
    15301 cgggtaacca ccaacccagg tcagccaatg cagcctattg ccaaagtcgc atccggtggt
    15361 gaattgtccc gcatcgcact ggcaatccag gtcatcacgg cgcgtaaaat ggaaaccccg
    15421 gcactgattt ttgatgaagt ggatgtaggg attagcggtc caacagcggc agttgtcggc
    15481 aaactgctgc gtcaactcgg cgaatcaact caggtgatgt gtgttaccca cctgccacaa
    15541 gtcgcgggat gtggtcatca acactatttt gtcagcaaag aaaccgatgg tgcgatgaca
    15601 gaaacacata tgcaatctct ggataaaaaa gcgcggttac aagagctggc gcgcctgctt
    15661 ggtggcagtg aagtcacacg taacacactg gcgaatgcga aagaactgct tgcagcgtaa
    15721 acttttttcc tgcttcacgg tcagagtaaa cagcaaaacg ccgtaagacc ggaaagcaaa
    15781 aggttttaaa gtgatgaaag gtctattatc atcggcatat tacagatgag ccacgtactg
    15841 ctcgggcccg aaaaggaatc aaatcactat gcgctgtaaa acgctgactg ctgcagcagc
    15901 agtactattg atgttaaccg caggctgttc cactctggag cgagtggttt accgtcctga
    15961 catcaaccag gggaactatc tgaccgctaa cgacgtatcc aaaatacgtg ttggcatgac
    16021 gcaacaacaa gttgcgtacg cattgggtac accgctgatg tccgatccat ttggtacgaa
    16081 tacctggttc tatgtcttcc gccagcaacc aggtcatgaa ggtgtaacgc agcaaacgct
    16141 gacgctgacc tttaacagta gcggtgtgtt gaccaatatt gataacaaac ctgcgctgag
    16201 tggtaactaa taataaagtt gctctcaaag acgttaaaaa aggtgctcaa tgagcacctt
    16261 ttttctgtct gttatttatt cgctgatttt tctgctcgtt gcctgcgaag ctctttcgga
    16321 tcggcaatga gagggcgata aatctccacc cgatcgccat catgcacact atcgcttagt
    16381 tttgccggac ggctgtaaat gccgactttg tttttagtta aatcgatatc ggtacgcaat
    16441 tccagcaagc cactggcgcg aatagcttct tcaaccgtcg cgccctcctg cagcgtcact
    16501 cgctgcaggt attgcttctc aggtagcgca taagccacct caacggcaat tttacctagc
    16561 actgtaaacc tctttcgcac gaaccgtaaa agcctggacc atattagccg ccagctcttt
    16621 aaacacgcga ccaaaggcga gttcaatcaa cttattggta aactcaaagt cgagatgaaa
    16681 ctcgatacga cacgcctcct ggctcagcgg cgtaaacttc catccaccaa tcaatttctt
    16741 gaacggccca tccaccagat tcataagaat actttggtta ctggtcaact ggttgcgggt
    16801 agtaaacgtt ttgctgatcc cagccttaga gacatctacc gccgcagtca tctgcccagg
    16861 agtggactcc agaatccgac ttccggtaca acctggcaaa aactgaggat aagactgaac
    16921 gtcattcact aactgataca tttgctccgc gctgtagggt accagtgcgg tccggctaat
    16981 ctgaggcata acaatttcca tcaacaaaaa tccaacaaat aatatcattt atcctgctgt
    17041 aaaaaaaacg ctatcccggc gctgggtaac atcgggttca tgctaagata gagccttgtc
    17101 ccccgcagga ttgatatggg gtgttttcga tttcagatta ccgatgattc acgacgctta
    17161 tgacgaagaa aaaagcacat aaacctggtt cagcgaccat cgcgcttaac aagcgcgccc
    17221 gtcacgaata ctttatcgaa gaagagttcg aagcgggact tgccctgcaa ggctgggaag
    17281 ttaaatccct gcgcgcagga aaagccaata tcagcgacag ctacgtcctt ctgcgtgacg
    17341 gagaggcatt tctgtttggc gctaacatca cgccaatggc cgtggcctcc acgcatgtgg
    17401 tgtgcgatcc tacccgtacc cgcaagttac ttctcaacca gcgcgaactg gactcattgt
    17461 acggtcgcgt caatcgagaa ggctataccg tagtggcgct ctccctgtac tggaaaaatg
    17521 cctggtgcaa agtgaaaatc ggcgtcgcca aaggtaaaaa acagcacgat aaacgttcag
    17581 atatcaaaga acgcgaatgg caggtggata aagcacgcat catgaaaaac gcccaccgtt
    17641 aaacctgcac tccaattatt gaccagttcc tcaccgcgcc tccctctccg gcggcgcgaa
    17701 tgaacatctt attggctatc acatccgaca caaatgttgc catcccattg cttaatcgaa
    17761 taaaaatcag gctacatggg tgctaaatct ttaacgataa cgccattgag gctggtcatg
    17821 gcgctcataa atctggtata cttaccttta cacattgggg ctgattctgg attcgacggg
    17881 atttgcgaaa cccaaggtgc atgccgaggg gcggttggcc tcgtaaaaag ccgcaaaaaa
    17941 tagtcgcaaa cgacgaaaac tacgctttag cagcttaata acctgcttag agccctctct
    18001 ccctagcctc cgctcttagg acggggatca agagaggtca aacccaaaag agatcgcgtg
    18061 gaagccctgc ctggggttga agcgttaaaa cttaatcagg ctagtttgtt agtggcgtgt
    18121 ccgtccgcag ctggcaagcg aatgtaaaga ctgactaagc atgtagtacc gaggatgtag
    18181 gaatttcgga cgcgggttca actcccgcca gctccaccaa aattctccat cggtgattac
    18241 cagagtcatc cgatgaagtc ctaagagccc gcacggcgca agccctgcgg gcttttttgt
    18301 gctccgaatt tgtcccgcga agtctgatgc caactaatta aatccgatcc ttttaggcac
    18361 cttgttaggc acttcataaa gctatattgt ctttgaggtg cctaaaacta tggaaacccg
    18421 gcaatggcaa gacaaaccaa acctctatcc gttaaggaaa tcgaatctgc taaacccaag
    18481 gaagcggact acgttctcta tgatggcgat ggccttgagc tactcattaa atccagcggg
    18541 agtaaaatct ggcagtttcg ctacattcgc cctgtcacca agacacgagc aaagaaaagc
    18601 ataggccctt acccgtcagt tacccttgcc gatgccagaa actaccgtgc agagtcccgc
    18661 tcactcctag ctaaacagat tgagccgcag gaacatcagc aagaacaatt gcgtagttcg
    18721 ctggaagcca aaaccaatac tttccagctc gtagctgaac gttggtggaa tgtgaagaaa
    18781 gccagtgtga cagaggacta tgcggaagat atctggcgct ctcttgaaag agatgtcttt
    18841 cctgcgattg gcgacgttag cgttacagat attaaagctc atacactggt tcaggccgta
    18901 caaccggttc aggccagagg agcactggaa acggttcgtc gcctgtgtca acgtattaat
    18961 gaggtcatga tctatgccca gaacacaggg ctgattgatg ctgttcccag cgttaatatc
    19021 ggtaaagcct tcgaaaagcc tcagaaaaag aacatgccca gcattcgacc ggatcagcta
    19081 tctcaactga tgcagacaat gcgaacagcc agcattagcc tttccacacg ctgcctgttc
    19141 atgtggcaac ttcttactat tacccgccct gccgaagcgg ctgaagctcg ctgggaagaa
    19201 gtagacatag aagcgcaaga gtggaagatt cccgcagcac gcatgaaaat gaaccgcgac
    19261 catactgttc cattgtcaga tgaagcaata gctgtgctgg agatgatgaa accgctaagc
    19321 ggcaaccgag aatttatctt tcctagccgt atcaagccca accagcctat gaacagtcag
    19381 actgttaacg cctcactaaa acgcgcaggt tttggtggtg tgcttgtttc gcatggtctg
    19441 agatcaattg ccagtacggc tcttaatgaa caaggctttc cgcctgatgc cattgaggca
    19501 gcattggccc atgtggataa gaatgaggtt cgtcgtgctt ataaccgcag cgattacctg
    19561 gaacagcgtc gcccgatgat gcaatggtgg gcaaattttg tgatggcagc ggatcgtgga
    19621 agcatgattg aaggtgggat aaaaggaatg aagctagttg ggtgactaaa tagctgcgcg
    19681 gcatagtgtc ccactttcag ccagcccttg caggttaagc caccagacag taaacggcgg
    19741 gtgtcgcgtt attaggcgca gctatttagt agtaatatta ggattggtga ttctgcactc
    19801 cagaagtt
//
LOCUS       DAJVRL010000077        19641 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVRL010000077 DAJVRL010000000
VERSION     DAJVRL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.077"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..3468)
                     /gene="mukB"
                     /locus_tag="OQN02_002750"
     CDS             complement(<1..3468)
                     /gene="mukB"
                     /locus_tag="OQN02_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1643478.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGA"
     gene            complement(3468..4172)
                     /gene="mukE"
                     /locus_tag="OQN02_002751"
     CDS             complement(3468..4172)
                     /gene="mukE"
                     /locus_tag="OQN02_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1643479.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4153..5475)
                     /gene="mukF"
                     /locus_tag="OQN02_002752"
     CDS             complement(4153..5475)
                     /gene="mukF"
                     /locus_tag="OQN02_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1643480.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(5472..6257)
                     /gene="cmoM"
                     /locus_tag="OQN02_002753"
     CDS             complement(5472..6257)
                     /gene="cmoM"
                     /locus_tag="OQN02_002753"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1643481.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            6393..7172
                     /gene="elyC"
                     /locus_tag="OQN02_002754"
     CDS             6393..7172
                     /gene="elyC"
                     /locus_tag="OQN02_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1643482.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7149..8042)
                     /locus_tag="OQN02_002755"
     CDS             complement(7149..8042)
                     /locus_tag="OQN02_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1643483.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8196..8942)
                     /gene="kdsB"
                     /locus_tag="OQN02_002756"
     CDS             complement(8196..8942)
                     /gene="kdsB"
                     /locus_tag="OQN02_002756"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1643484.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(8939..9121)
                     /gene="ycaR"
                     /locus_tag="OQN02_002757"
     CDS             complement(8939..9121)
                     /gene="ycaR"
                     /locus_tag="OQN02_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1643485.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9173..10405)
                     /locus_tag="OQN02_002758"
     CDS             complement(9173..10405)
                     /locus_tag="OQN02_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1643486.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(10442..11428)
                     /gene="lpxK"
                     /locus_tag="OQN02_002759"
     CDS             complement(10442..11428)
                     /gene="lpxK"
                     /locus_tag="OQN02_002759"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1643487.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(11425..13173)
                     /gene="msbA"
                     /locus_tag="OQN02_002760"
     CDS             complement(11425..13173)
                     /gene="msbA"
                     /locus_tag="OQN02_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1643488.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13210..15469)
                     /locus_tag="OQN02_002761"
                     /pseudo
     CDS             complement(13210..15469)
                     /locus_tag="OQN02_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(15676..15960)
                     /gene="ihfB"
                     /locus_tag="OQN02_002762"
     CDS             complement(15676..15960)
                     /gene="ihfB"
                     /locus_tag="OQN02_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1643489.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16120..17793)
                     /gene="rpsA"
                     /locus_tag="OQN02_002763"
     CDS             complement(16120..17793)
                     /gene="rpsA"
                     /locus_tag="OQN02_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1643490.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(17904..18587)
                     /gene="cmk"
                     /locus_tag="OQN02_002764"
     CDS             complement(17904..18587)
                     /gene="cmk"
                     /locus_tag="OQN02_002764"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1643491.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(18764..19525)
                     /gene="ycaL"
                     /locus_tag="OQN02_002765"
     CDS             complement(18764..19525)
                     /gene="ycaL"
                     /locus_tag="OQN02_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1643492.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4734 a         5432 c         4765 g         4710 t
ORIGIN      
        1 cgcacctaac gccttatccg acatggaacg caaatcatca gcggagagat aagccagctc
       61 acgacggtgt aagcggcgct caacgccgtt atctttcacc atgcgcatca ccgcacacca
      121 gcccgctttg gcggtcacta cctgctcgcg catctcaaag taatcccgct ccagcttgcg
      181 cagtttgcgg gtcaggttgt ccatctccgc ttcgcagaag gtaagcgctt tttccagttg
      241 attgcggcgt gaacggttat tgctcagttg cgcatgcagc tcgtcacggc gaatacgcgc
      301 ccgctcttct gccccgctat cagcacgcac gccgatatcc tgcaattcac gttgcagatc
      361 gttgagtagc tcttttttgg tgtcgtaaga acttttcagc gaagccagca cctggttgta
      421 ctgactcaac tgcgctgcgt gaccgcgcaa cgcttcgcga gcgcgggtac gctccgcttc
      481 cgcctgttcc agacgttcac gcagtttttc gttgagatcg ctgttaccgc taagcatttc
      541 tgccgagtca gaatagctaa agtgcgcacg acgctgcacc acttccgtca gggcaaacgc
      601 ttgctgacgg gcatcgcgct gcatctgctg agagtacgcg taatcttctt ttaactgttc
      661 gaactgttcc gggtcgctct gcaataccga aacgatcggt tccagtttcg ccagttgatt
      721 gccaaactgc tgaacaaaac gcgcggcttc ctgggcttca tccagacgtt cgcggatttc
      781 atcgacgcga tccgccaggc tgtcatcagc caacaggttg agacgcggca gaatgcggtt
      841 cagcgccgta acgccttctt tcgcctgctc aaactgaata cgctgctgct ggttatcatt
      901 ttcatgatta ctcaacgccc gctccagttc gactcgacgg ctgttcagtt gacggatttc
      961 tgcttccggg tcagactcaa acgccaccgc cagatgactg ccgataaagc ggctgaacgc
     1021 ctgatgcaga cgctgggttt tctgtacatc aaaggagagc gtggcgaagc gttcggaaag
     1081 cacttcacgc tcggcatgga ggctttcaat acggctttca cgcgcagcac gaccaaacag
     1141 cggcacttcc gggaaacgtg aataacgcca ctgtcgatcg gcgattttca ccaccaccgc
     1201 tttttccagc tcatcaacgc tgaacacgct atcatcgaat gactgcggat ccccttcgat
     1261 caaatagaga tcttccgggc aatcggtcaa gccttccagg tgttcggtta cctgtgacag
     1321 atctggcacc acgatggcgt ggcgtgacgg gccatacaga gccgagaagt acggcgcatc
     1381 ttccaggcta acgtcgtcat aaatttctga cagcagcaca ccaccaaaac gctccgccag
     1441 cgcgttcaga cgctgatctt cagagccgcc aggctggctt aaacgttcga tctcttcatc
     1501 gacggcgttt ttgcgcgcgc ctacttcatc gcgttcaaca atcgcctcgc gctcacgctc
     1561 cagcaactgt tgcagatatt cagtgacgtc ctggctggag gagaactctt cgccgcactg
     1621 ttcactcaac tggttgagac tgttttgcgc tgccagccaa accggtgcac gctgcatcaa
     1681 actctgaatg cgagactgca gctgttcctg ttcctgccgc agtgccatcc gctcttcacg
     1741 ggcgttagat acgctatcgg aaagagaggc aatgcgtgct tccagctcct gatgcagagc
     1801 ttccagttca tcaatatcaa aattcttgcc ctgacgtttg cagaaatctg ccagcagacg
     1861 ctcagcttct tgctgctcac gcagacgctg ttccagttcg cttaagcgca tccgcaacgg
     1921 ctgaacctgc tctgccaggt gacgctgatc aaccccttcg cgcaatagtt cgcgagcgac
     1981 atcccacgcc tcgttacgcg ccagcgggcc gttgattgcc accaccagct gataagcctg
     2041 ctcaaactgg ctgtgcgcgg tttgcgccat gctcattttc tgctcaagag agagcatttt
     2101 ttcagtcgct tccagctctt tcgcctggaa ggtttccagc cattcggcgg cgctgtcggc
     2161 ggttaagtcc ggcagatggc acagttcttt ggcacgatta agcgcagcaa tcgcctggtt
     2221 atactggatc gcgcgcgttt gctggacgtc cagcgcctgc tggtagtcag caagctggct
     2281 tttcagctcg tccacttcca gttcggcagc ttccgcgcga gcctcattct cttcctggcg
     2341 ttcgatggct tctgccacca cttcattttg ctcttccaga cggatctgca gctcatcgag
     2401 atccacttcg tagcgttcga tcttctcctg ctgacgcaat gcggtttgca ccaggttcag
     2461 gtgatcgctg gccgcctgat aatccgcttc cagatcgcct tcggcaccgt tgtgctctgc
     2521 cagctcacgc gccatatcga cgtgtttgta ctgctcagcc gccagttgct gacgcgaagt
     2581 atgtagctcg cgacgaaact ccagggcttt gtcgagatgg acacggcgct cgttggcgtg
     2641 acgcatgtag tccgccgcca cgtagttggt agcttcgctg atcagatgct taaacaggtc
     2701 acggtccgac tgggtgacac gaatcgcttc cagcgtcatg cggttttcac gcagcgccgc
     2761 ttccatgtcc tggaacgctt tacgcacgcc gctgttttcc ggcaacaggt agtcgcgcag
     2821 agaacgggta atggcactgg agatcccgcc atacagcgaa gcttcgatca gacgatagaa
     2881 tttgctacgg tcagatgcgg agcgcagacg acgcgcgatg atgcccagat cgaacatcag
     2941 cgagtggtaa tcggtaatgg agttgaactg tttaaactgc acgccttcca tcgcctcgag
     3001 cttgtctttc agctcgttaa gcggcagcac gcgcgcctgg cgttcgttca gggtttcggt
     3061 caccagctgt gtcggctgca ccgacatcgg cagtccctga atggcaaatg gtttgatatc
     3121 cactttacga tcgcgtccgg caacctgttg cagacgcaca ccgaccacca cgcgctggtg
     3181 gcgcgagtta atggtgtcga gcatcgaata acagacaccc gctttcagct taccgtgcag
     3241 acctttatcg cgcgaaccgc tggtggcccc ggcttccgta gtgttacgga aatgcagcag
     3301 ggtcaggtcg gggatcagcg ccgtaacgaa cgccgccatc gtggtggatt tacccgcccc
     3361 gttaccgccg gaaagcgtcg tgaccagctc gtcaaggtca aaagttcggg caaaaaagcc
     3421 gttccagtta atcagcgtca gtgagcgaaa tttaccgcgt tcaatcatta ttcttcctct
     3481 ccgctatctg gctgactctc ttcggtttca tcgttgagtt gcagatgatt ttcaatcggc
     3541 attgcttcgc catcacgaat caggcgacgc tgtgcttcac ggggatcgtc gccagcacgc
     3601 acatcggctc cgaagcggaa caccgattcg gtaatgcgga acttgctgct gtcgtggccc
     3661 ataaaccaca ccatgcctaa acgacgcaga cggttgagcg aagaacgtac tttctcctgc
     3721 aacttctgac ggtcaacgtc tgaaccggtt gaacggttgt tcaccagttt cagcagtttc
     3781 gcttcatcgg ccagggtgag cagttcgtcg tacagttcct gctgggtgaa aatcccctca
     3841 ttcgccagcc gttccgggct gagatagaga taacagagga ttttcccgac catcatatcc
     3901 agttccgaca agacggaacg cgggataaga gtggtggaac gtgggcgtaa atagaagaac
     3961 ccttccggtg cgcgaataag ctcaacgtta taacgcgcgt aaaactcttc cagatattcc
     4021 tgaaaatcca tcaggaatgc atgattatcc agttcgtcga ggccaatatg gcggcctgaa
     4081 cgtaaggcgc tgtccagcgc cggaaataac ggattcgcca gcgcctgcgc cagcttaacc
     4141 ggcatcactt gttcaatatt tgtcgatgac atgcgcctgt accttggctc cgtaatcatt
     4201 aatcggctgc catttcgctg gcagtccggt gaaatctgct tgcgctacgc caagacgtac
     4261 cgcctgatca ataacaatac gcgcaacgtc aaagtgacgt gcacgcggat actgtgacag
     4321 atattcgcgt accaccagac caagatccag cggcacttgt ctggttttgt acacggcaag
     4381 ttgttcttcg atgatcgccg ccagctgttc gcggatctcg ttaaactctt cgtattccag
     4441 atcctcagga agttccccag tcacttcttc atcgcgcagt gccatctctt cgtcacgcat
     4501 atccagcaga cgatcggcat tggcataagt tagcgcccac ggctcatcaa aataggtttg
     4561 taccgactga cgtaaccgct gagcaaagac gcggttttta tccatatcga tcgcggtacg
     4621 aataaatttg tgtacgtggc ggtcgtagcc aatccacaag tcgatggatt gctggcccca
     4681 actgataata cgatcgagtt tgctctgcag atcgaacacc agacgatcga cgaaatgcag
     4741 atcgtcatgg gtcatcgtcg catcctgaat gcgcaacaga ttagcctgca atttgtcgcc
     4801 tgccgcttcc agcgtatcct gcaattcacg cagcgttccg gaagtttccg aaagcaacaa
     4861 ttcacagctg gaaatcgccg cccgccagtc tttgttcagc aactgggcga tatcgtcctt
     4921 cacctgctgc tgctgttcgt ccatcagacg ttgcgtcagg tcgatactgt cgaaaatttc
     4981 tgctaccgaa tatttcagtg gcgcatagac attacggtgc cagtgaaatt catcaccgcc
     5041 ctcttcggcg gcatctgctg cgcgtttgag ctcacccgcc acaatcgaca actgcataga
     5101 aagacgcagc gtagaaaact cgcgctgacg gatgtagtag tcagtaatgc cgatgccgag
     5161 cggcgtcaga cggtaaattg cgttcccttc cgcctgctcg ctggtaaagc ggttcagcag
     5221 acgttgacgc accatgtcgt tgatcgcgtt attggcgcgc acgccgatgg tttcgctggt
     5281 ttgctcaaac gcatcactca catggcggaa tgcatccacc agctcgcctt cactcatctc
     5341 accatccaga cgctcgccgt tcagcgtggc aaccgccagc agaaaagaga gtcggtctac
     5401 cggcagcgag atggagaagt cattttttct ggcccaggca accagttcgg ggactgtctg
     5461 ggaaaattca ctcatacttt atccttgctc tgcggtttgc gcgcggtgac atgaatataa
     5521 cgccctaggg taatatacgg ttcctgacgg caataacgcg tttctaattc aagtaatgct
     5581 tcatagcagt cgcgctgctg gtacttctcg cgcagataat catgaaacac gcgaacgcct
     5641 gtcttaccca taatttgcca accagcttct tccagccaca gataaacctg cgccgggtcg
     5701 cgtggataat ctggcgaaag cgtccgtttt ttctttttcg gcattcccgc ctgcacgtaa
     5761 tcaaaattcc cggcgaccat gttatgcatc aacaaaccat gcgcattgta gaacattaac
     5821 gacaacactc cgcctggacg caacactgac cacagggtct gcaatacact gcggggatcg
     5881 gccacccact cgagcaccgc atggaacaat atcagatcaa cgggcgtttc caaatgcgaa
     5941 gcaacatcct gagcggcgca atgtataaat tgcatgttgt cgctcacacc ttttgcttct
     6001 gccgcctgtt ttgcgcggtc gatcatctgc gcagaaagat cgcataaaat aacctgatgc
     6061 ccgcgctcgg ccattttgat tgcggtctgc ccttctccgc cgccagcatc cagcacacgc
     6121 agtttttgcg ggcccatttc cgccagcacg cgatcgagat cctgccacag aatagcctgt
     6181 cgaagctgcc ctttggtggt gccgtaaatg ttacgggaaa acttttccgc aatatcatca
     6241 aaattgcgat cctgcattgc tttctccacc ggttggctaa gacgttttct gataaagacg
     6301 ctattttgtc acagccacgg cgagaatgaa cccgtctggt gtaatatcgc tagcgatccc
     6361 ttgctatatg gttaaaaaag gaaccagaaa ggatgctttt tacactgaaa aaagtgattg
     6421 gcaatatgtt gctgccactt ccgctgatgt tgttaattat tggcgctggc ctggcgctgc
     6481 tgtggtttag ccgttttcaa aaaaccggga agatttttat tagcattggg tggctggcac
     6541 tcttgttatt gagtttgcaa ccggtagccg accgcctgct gcgtcccatc gaaagcacct
     6601 atccgacatg gaataattcg caaaaagtgg actatatcgt ggtgctcgga ggtggttata
     6661 cctggaaccc gcagtgggca ccgagctcta atttaatcaa taacagtctg ccacgcctga
     6721 atgagggtat ccgtttatgg cgcgaaaatc cgggttcaaa actgatcttc acgggaggcg
     6781 tagcaaaaac caatacggtg agtacagcgg aagtaggtgc cagagttgcg caatcgctgg
     6841 gcgtaccgcg cgagcaaatt atcaccctgg atttgccaaa agataccgaa gaagaagctg
     6901 cggcagtgaa acaggcgatt ggtgacgccc ctttcctgct ggtgacctcc gcatcacatc
     6961 tgccgcgggc gatgatcttt tttcagcagg aagggttaaa tccgctacca gccccggcga
     7021 atcagttggc catcgactcg ccgcttaatc cgtgggaacg ggcaatccct tctccggtct
     7081 ggctgatgca tagcgatcgt gttggctatg aaacgcttgg ccgcatctgg cagtggctga
     7141 aaggtccgtc aggcgagcca cggcaagagt gattttgatg ccagatcgaa gttgcgccga
     7201 ctaaatcgtc cggtattaac cagttgcgca acttcatccc ataacacata caaccaacgc
     7261 cgccagatga acgactccgc cactggcgcg cgttgcaggt aactccagag caaatcttcc
     7321 gccagagaat tatccattag tcggaacagt tcgtattctc gcggtgccca aagcattagt
     7381 cccggcccga ccatcgccag taactgatcg ctgcgcgaat ctttcagcat gctgcgtaaa
     7441 cagaagttac cgtgaatcaa cacacaattg tcgttaaagc cttcaaataa tgccgggaga
     7501 cattcgcgag tgcgaaacag gatccgctta tcctgcatcg tcagaccggt gttattgaac
     7561 tgattgagcg tggtccatag cacttcaaca tgttgccggt accatgaggg ccagaaattt
     7621 tcctgagtat tgtcgaccgc gccgacgcaa ccgcgactgt cctgacggtg ccaggccagt
     7681 aaggcttcaa cgatttggtc tttgagctgt tcccagcgtt ctggtgttcg ggctggtgcc
     7741 tccaccgaaa caccacgcat ccgctccagt agcaggacat cagggccggg gtgttcttca
     7801 tgcgtcatca caccataaat agtcggcata cggacagtcc cacttcttgc cagcatggtg
     7861 gttttccatg ccagttgtcg ggcttttccg ggcgtactaa agcttcttgc cattaacggc
     7921 attgggtttc cctggctgtc atacaaagcc cacaacgccg tatccgcttt ttcattgacg
     7981 cactcaatac gactgagttt ttcgcccagt aaatggctta attcggcacg cagctgttcc
     8041 attcagatta ccctcataaa agctactgct ttcataatga gcgccaatat aaaagatgtc
     8101 acctgaatga gatcaattgt tgaaaagaaa aattgaggaa aatcgtaacg gtacgacact
     8161 cctcccaaaa ttggctgaag tgtcgtgaag tgaaattagc gcatttcagc gcgaacgcgc
     8221 tcaagatctt caggggtatc cacacctgtg ccaggaactt cctgagcaac agcaacatgg
     8281 attttttcgc cgtaccacag aacacgaagc tgctctaaca tttcgatgtg ttctaacgga
     8341 cttgcctgcc agttgacgta acgacggata aagcctgcac ggtagccata aataccaaga
     8401 tgacgcagga agttatcgcc aacggtttca aggccttctg caaaacgatc acgatcccaa
     8461 ggaatggtgg cacgtgagaa gtacagtgca tacccttcag cgtcgagaac cactttcacc
     8521 gcattcgggt taaacgcttc ttccgcattg tggattggca ccgccagagt cgccataccc
     8581 acctgacgct gagcgaggtt atcagcaacc tgacgaatga ttgtcgcagg gatcatcggt
     8641 tcatcaccct gcacattaac gatcaccgtg tcgtcgctga atgcgcattt ttcgacaact
     8701 tccgccagac gttctgttcc tgactgatga tcggcgcgcg tcatacatac ttcaccgcca
     8761 gcggcttcaa cggcgcgggc aacatcatca tgatcggttg ccacgatgat gcgctcggca
     8821 cctgattcac gcgcgcgttc aagaacatga acaatcatgg gtttgccgtt aatatcaacc
     8881 aatggtttac cgggcagacg cgtggacgcg tagcgcgcgg gaataatgac cacaaaactc
     8941 atgatttact ctcatcagca gtcagcacgc gggcttccgt ttccagcaac actggaatgc
     9001 catcgcggag cgggaaagcc aggttatcca gtttgcaaat aagctcttgt ttttcctggt
     9061 tataccaaag ttttccgttg caaaccgggc aggcaatgat ttcaagcaaa cgatgatcca
     9121 tagttcctcc agatggatcg ggttatgaat gcataaatct tatcataatc atttatgcga
     9181 cggggtctat ttcccagcca gtgcggcaat cagtaaagag accttgcggg cagcgtccta
     9241 atgtcacccg cgttgcctgc tgccagctcg cgaaatcagt aatcgcctga cgtaaccctt
     9301 tttgcagcat tgtcgttggt ttaatgcctt cctgtaacca cagagagata acttcaagga
     9361 tgcctgtctg gcgatgcatt ttggcatcca ttcgcccaac taattgccca cgatgtaata
     9421 atggcagaac aaaatagcca tactggcgtt tcggcgctgg ggtatagcac tccagccggt
     9481 agctaaaatc aaaaagctgc tctgcgcgtt tgcgatccca gacaacagga tcaaaaggcg
     9541 aaagtaccgc gctgtgcgtt gcagtgagct ttccggccag cgcacgctcg agtagcggca
     9601 gcaaatcagc atgcagccaa agattgccca atttttcaac atgcacagca atgatttgct
     9661 gctgttcagc cctcgcttcg cgccacgccg ccagtgccgg acgtttcagc cgatagtaat
     9721 ctgccagcca ctgttcgcgg aatattccca ggctacgcgc actgttatcc agcatgatga
     9781 tttctgcttc tgtttgcgaa acgagatcgc gctcatcatc ccagtcaggc atgacacggt
     9841 gggttaaatc ataaacgcgc tggaagttgc gccgttcaat caccatcacc tttccggcag
     9901 taaataaacc ttccagatgc cgtttatgcg gcttccattc ccaccagccg cttgcacctt
     9961 tacgaggatg ctcaaaatcg gctgaacgta ccggcccttt atcatgaata tgctgaatta
    10021 actgtgcaat ttccgcctca tgttcctgca tccaggcgtc tttgtatttc cagcccattt
    10081 tttcaggtgc cagcatgcgg tggcgaataa gacgaaagtc gctacgcggc ataaagcagg
    10141 cttcatgcgc ccagtattcc attaattcgc cacgcgccag agactcatcc agccactggg
    10201 caggataatt tcccagacga ctgaaaagca ccagatatgg actacgggca acaatattga
    10261 tggtatcgat ttgcagcaag gacatgcggg agatcgttgc cggaatatcc tccaacgacg
    10321 ctcgacggcg gggtttgttt aacaggcctt gtgcggcaag gtgaagatta cgcgcatcag
    10381 caagggagag gtgcggcagc gacattcatg actccatcaa tcgaacgctg ccgcggcgta
    10441 actagttgcc agaagccagc gaggttagtt gcgtaagcag tttcgctggt tcatcacctg
    10501 aaagctgtgc gtctacaggc aaataccacc aattttcttc tgcaaaggcc cggcatttca
    10561 ccgcatcttt ttcagtcatt accagcgttt gcccggcgct taccaacgca ctgacatccg
    10621 catggttcaa agactgatga tcggccagcg gtacacattt ttccggttgt acgccacaca
    10681 tcttcagcgt ggcaaaaaag cgcggcggat gcccaatccc cgccatcgcc actacatgtt
    10741 caagctgagc aacgtcacaa cgcgtaccgg tacgtaaatt caccgcctga cccggcagca
    10801 gatgcatggg gatttcaccg ctgcgaggga caccgccgtt gacgattacc gcatcaaccg
    10861 actttaagcg ccccgctcgc tcacgcattg gccccgccgg caaccaccag ccattgccaa
    10921 agcgacgcac accatcaata acgacaattt ccacatcacg cgccagacgg taatgctgta
    10981 aaccgtcgtc ggttacgatg atctgcacat cagggtgttg cgccagaatg gcttttaccg
    11041 catcagaacg aacgggagaa accgcaacag gcgcatcagt gcgttgataa atcaacacag
    11101 gttcatcacc cgcctgtgct gtggtggtat ctgccgacaa taacagcgga taagattcag
    11161 ccttaccacc atatccccgc gatacgaccc ccacgcgaat accgcgctgt tgcaactgtt
    11221 ccaccagcca gacaacgacc ggggtttttc cgttgccgcc tgcggtgaga ttaccaacca
    11281 cgacaaccgg tacgggggca cgccaggcgc gcttcagttt tagtttatag caaagacgga
    11341 tcgcgccact caccaggcca tacaaccagg agagtggcag caatagccgc cacaaagggg
    11401 attcaccaga ccagattttt tcgatcattg gccaaactgc attttgtgaa gttgcgcgta
    11461 aacgccgcgg tgctcaagca aatcgttatg cgtaccgcgt tccacaatga caccatcctc
    11521 gacgaccacg atttcgtctg ccttttcaat ggtagacaag cggtgggcaa tcaccagaga
    11581 ggtacggttt ttctgcaact catccagtgc cgcctgaatc gcacgttcgg attcggtatc
    11641 cagagccgag gtagcttcat ccagaatcag aatcgggcta tcacgcagca aggctcgagc
    11701 gatagcaata cgctgacgct gaccgccaga gagcagcacg ccgttttcac caatcactgt
    11761 atcgagaccg ttatccatct tattgatgaa gtccatggcg taggccatcc gcgccgcttc
    11821 ttcaatttgc tcacggctgt actgttcagt ccgtgcgtaa gcaatgttgt tagcaaccgt
    11881 atcgttaaac agatggacat tctgcgacac cagagcaacc tggttacgta acgacgccag
    11941 ggtatactcg cgcagatcgt gaccatccat caggatttcg ccttcatcaa tatcgtaaaa
    12001 acgcgtgatc aggctggcga tggttgattt acctgaacca gagcgtccaa ccagagcaac
    12061 cgtcttccct gccggaattt tcaggttgat gttacgcaat gcgggtacgt cacgtcccgg
    12121 ataagtaaag gtgacattgc ggaattccac gtcgccagtc gcacgctcga tcacgcgctt
    12181 accttcgtct ttctcctgct cactgtccag aatggtaaac agcgtctgac aagccgccat
    12241 accgcgctgg aactgggcgt taacgttggt cagcgatttc agcggacgca tcagtgcaat
    12301 cattgaagag aaaacaacgg taatcgtacc ggcagtcagg ttatccataa cacttgggaa
    12361 gctcgccgca tacagaacaa acgccagcgc caaagaggcg atcagctgaa tgatcggatc
    12421 agagatggaa gaggctgaaa ccattttcat cccctgaaga cgcattcggt tactgacttt
    12481 atcgaagcgt ttcgtttcca cttcctgacc accgaaaatc aatacttctt tgtggccctt
    12541 cagcatttgt tctgcactgg tggtcacttg ccccatggtg ttctgcatgt ttttactgat
    12601 gttgcgaaaa cgcttcgata ctacgcgaat cgcaatcgaa acaatcggtg ccagcacaat
    12661 caaaatgatc gacagttgcc aactgtaata gaacatcatg atgaacaggc cgatgatcga
    12721 cgcaccttca cgcacaacag taatcagtgc gccggaagaa gaagaagcaa cctgttcgga
    12781 atcgtaggtg atacgtgaca acaacgtacc cgttgactgt ttgtcaaaga atgaaactgg
    12841 cattcccatc atgtgaccaa acaggcggcg acgcatggtc attaccacct ttcctgatac
    12901 ccaggagata cagtagctgg agacatagct ggtgatacca cgtaaaatca tcagtccgat
    12961 caccaccagc ggcatccaca ccagcacgga gcgatctgtt ttaccaaagc catcatcaag
    13021 aagtggctta aggagcgata acatgaaggt atcgctggct gcgttgagga ttaacgctac
    13081 gcccgccacg atcagacccg ctttgaaagg cgcaatggtt ggccacagtc ggcggaatgt
    13141 ctgccacgta gagagatctt tgtcgttatg cattcaaaaa accagcattt gttgaaatag
    13201 ccgcatattc tacccgttat ccactggcac gccaaaccac tgatgatacc aacgaggtaa
    13261 aatttgctct ctgaggctgc taatccgcca gccttgtgct gaaaaattga ccgtcacttg
    13321 accctgatgt ggagtatcaa gccattgata tcctttctgt tgatagcgat gcttaacttt
    13381 gttagagggc aatcgccatg cgttatagcg cgatgccgat gcgagtgcca cttttccatt
    13441 cactcgctga attaatggca acgatgatga ggtattactg ccatggtgag gtacctgaag
    13501 caatgttgcc tgcacttgct gccagtaacg gcttagcatc ttttgttcag ctggggcttc
    13561 aatatcaccg gttagaagaa tgctattcgt cccgtcatca accttaacca cacaggaatg
    13621 gttatttccc ttatcgttgc taccttgtaa aggccagtgc gcgctgaaac gcaatccttg
    13681 ccactgccac gcttcgccac gcacacaggg ctgatgatgt tcccagttta acggacttct
    13741 gatccataac atcggccata tatgcaatat tgaatccagc cctccccggt gatccagatg
    13801 ttcatggctc agaataacgc cttccggttc aagattatgc cagtggagcc aggggataat
    13861 cagttgttgc ccactatccc cttcaggcca gtcctgtgtc atagagaatc gctttgccgt
    13921 ttctggctat caccattgcc agcccttgcc cgacatcaag catgtacagc tgccactcgt
    13981 caggtcgagg tttttgccac agcggccagc acatcagcaa gcctccagcc acacacattg
    14041 ctggcaacgt tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg
    14101 atagccattg ccaacattca acaatgttga tccacccttc cggcaaactc tttaaccccc
    14161 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccctgttct aggattaacg
    14221 gcccgctaaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
    14281 agggaattgc aaatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
    14341 tttgcatggg cataagcagg agtgtgattc ccagttgcag atggatgagg gaaacaactg
    14401 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
    14461 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
    14521 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
    14581 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
    14641 ttcccgtcaa ccaggcataa aaagcagcac agcagattcc gccaattaat ggtatttgcc
    14701 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
    14761 acgccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
    14821 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
    14881 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
    14941 gcaggctaca attcggttca atgacacttg cctgcagaaa acggccggtg agcggctgat
    15001 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttgg ccatgaactg
    15061 cacgaacttt gagtttcatt gaccattgtt gtccagcaca aaccgcttgc ggaagttatt
    15121 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
    15181 gaccgtaatg agtggtcatg ccgtcagttg cagtgatctc aacaattgca tcctgcgtcg
    15241 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
    15301 aaaacagcaa cgtcagcgcg atataacgga cggttttaac agggataaat gccagtacgc
    15361 aggcgaagag agtcagaaac gcaagggtta atgtcctagg caattggggc aaaatcagca
    15421 acggaaaaat tccgcaaatt atgcatacgc cgactgtcgt tattttcatg cacccctccc
    15481 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
    15541 gaggcacatt cagaagaatt taacgaaagg gtgaaaactg ctcaaaactc tgcgaggcag
    15601 cttccagttc ctgattagag aattgaaaaa agcacccgac aggtgctttt ctcgcgttca
    15661 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
    15721 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
    15781 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
    15841 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
    15901 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
    15961 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
    16021 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
    16081 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
    16141 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
    16201 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
    16261 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccagggt
    16321 agcgtcttca acgcggtcac gggatgcttc agaagcacgc aggtaacctt caacgccgtc
    16381 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
    16441 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
    16501 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
    16561 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
    16621 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
    16681 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
    16741 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
    16801 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
    16861 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
    16921 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
    16981 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
    17041 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
    17101 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
    17161 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
    17221 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
    17281 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
    17341 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
    17401 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
    17461 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
    17521 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
    17581 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
    17641 ctcagccggg atagcggact cagatttcag accagcgtca accagtacta cgtctttgtc
    17701 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
    17761 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
    17821 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
    17881 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
    17941 gctttttcaa tcaattgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
    18001 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
    18061 gccaaaaggc gctcgaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
    18121 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggtgcatc agggaatacc
    18181 accgttccca tgtcgcggcc atcggcaatc agacctggta attcgcgaaa cgcgcgttgg
    18241 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc cgcattcgcc
    18301 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
    18361 ccattggtcg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttcc
    18421 gacgcaacat caacatggtg atgtaatgcc gccaatgcca gtacgcgata aattgcaccc
    18481 gagtccagca gatgccattg caacgcttcc gccatagcct tacacaaggt gcctttccct
    18541 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
    18601 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
    18661 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
    18721 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttagcca gaggcgatac
    18781 gatcacggat gtgttgcgca cgctctgttg atggtgggtg agagtcaaac atggactggg
    18841 tgcgaccgcc atccaggcta gccagttttt caaagctgcc aaccagcccc tgggtgctga
    18901 tgccacgttt cttcaacaga tcaaaagaga aatcatctgc ttcggactct ttatcacggg
    18961 agtatttagc attaatagcg ccttctgcga tatcacccaa ttgtgagcgg gaaagctggg
    19021 aagccacacc gctggtagct gaaatggcat cgcgtgcggc aacgatcgca taagaagctt
    19081 tcatttcagc cagcgagtga cccaacgcga cgtggcccag ttcatggccc agaacacctt
    19141 caatttcgtt gtcattcatc atgtccatca ggccactgta gacacgaaca cagccgttcg
    19201 ccatcgccca tgcgttgacg tcgctggtca tataaacctt atagttgacc ggcgtgccat
    19261 taatattgtt acccaatgct ttggcgattt ttgccagacg tttaccgtac ttgctggatt
    19321 tacttgcgac ttgattgccg ctgtccattt cggcacagcc ctgattggca atcgcttttg
    19381 catcggcatc gcttaatgtt gctgctttat aggcatttaa accggagctg atagccatat
    19441 tggtatcaat accgtgggta ttttgacacc ctgtcagtaa tgctgcagag gtcgcaatcg
    19501 ccagcagtaa tttagtattc ttcatgtgaa tcatccgttg attaatcata tataaatatt
    19561 atgtatcaac aacttacatt gacaagcgac gctaccatta atcaaaatga ctcacaaggt
    19621 ccgaaaaaaa acgccgacaa t
//
LOCUS       DAJVRL010000078        19414 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVRL010000078 DAJVRL010000000
VERSION     DAJVRL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.078"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="OQN02_002766"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="OQN02_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="OQN02_002767"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="OQN02_002767"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1643493.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OQN02_002768"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OQN02_002768"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1643494.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="OQN02_002769"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="OQN02_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1643495.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="OQN02_002770"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="OQN02_002770"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1643496.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="OQN02_002771"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="OQN02_002771"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1643497.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="OQN02_002772"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="OQN02_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1643498.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="OQN02_002773"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="OQN02_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1643499.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="OQN02_002774"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="OQN02_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1643500.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="OQN02_002775"
     CDS             11402..11617
                     /locus_tag="OQN02_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1643501.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OQN02_002776"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OQN02_002776"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1643502.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="OQN02_002777"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="OQN02_002777"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1643503.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="OQN02_002778"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="OQN02_002778"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1643504.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="OQN02_002779"
     CDS             complement(16353..16892)
                     /locus_tag="OQN02_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1643505.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="OQN02_002780"
     CDS             complement(16908..17426)
                     /locus_tag="OQN02_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1643506.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17737..17928)
                     /locus_tag="OQN02_002781"
     CDS             complement(17737..17928)
                     /locus_tag="OQN02_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS1643507.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17946..18098
                     /locus_tag="OQN02_002782"
     CDS             17946..18098
                     /locus_tag="OQN02_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643508.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18280..>19414
                     /gene="pdeF"
                     /locus_tag="OQN02_002783"
     CDS             18280..>19414
                     /gene="pdeF"
                     /locus_tag="OQN02_002783"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1643509.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4568 a         4729 c         5277 g         4840 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact tgtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acgcagggaa taatttataa aaatatatca ttacaacata tatattaatt
    17581 ttatatttat ttcgcagcca aacaggataa acatcagtca ggacaaagac gtaaaattta
    17641 agatgaaaga atatctgccg ctttgataat cccaaagagc aaaagcatcc gacagcgaag
    17701 tgccgttatg ttctgtgaaa gtggagaagg taaccgctaa cgttgtaccg gtgattcgac
    17761 ggagagtgtg gattgctgag cttctttttt gctctgatgg tgctgccagg caccgacgga
    17821 cgagtagata aaacggccaa agaagaagat aaagctgatg agcagtacga tacgggtcat
    17881 gcgactgtta aatcggtgtc gttttcgcat actagttgcc tgactcacaa aaggttcctt
    17941 gaagtatgtc ccacgctgtg gacggtactt acattaaggc acaacaggac aaaatggtca
    18001 attcttcgtt atgtaaaaaa gcgtcagtgg atacatattt taatgttatg gaagttaatt
    18061 taattattta caatgatgat gtaataatga tgaattaaca tgataaacgt aataattcat
    18121 taatcagggt tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc
    18181 aatgctctct atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat
    18241 acacctcctt caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat
    18301 ataaaaatac gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc
    18361 attttcgccc acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat
    18421 ctgcctttgg cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg
    18481 atcaccttag ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca
    18541 ttaacggcta attttattat cattatcatt ctctgttggg gcggttacag ggtctttact
    18601 ccccggcgta acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg
    18661 cagatagtgt ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga
    18721 ttactggcga gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc
    18781 aattatcagg ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt
    18841 cgggtagtgc gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt
    18901 gatgccaaag tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg
    18961 ttgctgttat gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg
    19021 tcattattgc tgcccctgat gatgtgggga gcaatgcgct atggttataa gctgatttcg
    19081 ctgctctggg cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt
    19141 tatcctggct ataccacgca gctgaccata acctcctcca gttatctggt attctctttt
    19201 attgtcaatt atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag
    19261 cggcttgcgt atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg
    19321 ttgcgtgatg ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg
    19381 gttaagaact atggcatcat gctgcggatt caat
//
LOCUS       DAJVRL010000079        19262 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVRL010000079 DAJVRL010000000
VERSION     DAJVRL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.079"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            32..331
                     /locus_tag="OQN02_002784"
     CDS             32..331
                     /locus_tag="OQN02_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1643510.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            328..2076
                     /locus_tag="OQN02_002785"
     CDS             328..2076
                     /locus_tag="OQN02_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1643511.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2365..2643
                     /locus_tag="OQN02_002786"
     CDS             2365..2643
                     /locus_tag="OQN02_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643512.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2640..2945
                     /locus_tag="OQN02_002787"
     CDS             2640..2945
                     /locus_tag="OQN02_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643513.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2955..3116
                     /locus_tag="OQN02_002788"
     CDS             2955..3116
                     /locus_tag="OQN02_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1643514.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3258..3482
                     /locus_tag="OQN02_002789"
     CDS             3258..3482
                     /locus_tag="OQN02_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643515.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3783..4940
                     /locus_tag="OQN02_002790"
                     /pseudo
     CDS             3783..4940
                     /locus_tag="OQN02_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4980..5552
                     /locus_tag="OQN02_002791"
     CDS             4980..5552
                     /locus_tag="OQN02_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1643516.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5554..6765
                     /locus_tag="OQN02_002792"
     CDS             5554..6765
                     /locus_tag="OQN02_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1643517.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6762..7100
                     /locus_tag="OQN02_002793"
     CDS             6762..7100
                     /locus_tag="OQN02_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1643518.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7097..7393
                     /locus_tag="OQN02_002794"
     CDS             7097..7393
                     /locus_tag="OQN02_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1643519.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7393..7833
                     /locus_tag="OQN02_002795"
     CDS             7393..7833
                     /locus_tag="OQN02_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1643520.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7817..7999
                     /locus_tag="OQN02_002796"
     CDS             7817..7999
                     /locus_tag="OQN02_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643521.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8055..8345
                     /locus_tag="OQN02_002797"
     CDS             8055..8345
                     /locus_tag="OQN02_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1643522.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8365..10026
                     /locus_tag="OQN02_002798"
     CDS             8365..10026
                     /locus_tag="OQN02_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1643523.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10040..10321
                     /locus_tag="OQN02_002799"
     CDS             10040..10321
                     /locus_tag="OQN02_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643524.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="OQN02_002800"
     CDS             complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="OQN02_002800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1643525.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="OQN02_002801"
     CDS             complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="OQN02_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1643526.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13418..14788)
                     /gene="purB"
                     /locus_tag="OQN02_002802"
     CDS             complement(13418..14788)
                     /gene="purB"
                     /locus_tag="OQN02_002802"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1643527.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="OQN02_002803"
     CDS             complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="OQN02_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1643528.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="OQN02_002804"
     CDS             complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="OQN02_002804"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1643529.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="OQN02_002805"
     CDS             complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="OQN02_002805"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1643530.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="OQN02_002806"
     CDS             complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="OQN02_002806"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1643531.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17925..19175
                     /gene="icd"
                     /locus_tag="OQN02_002807"
     CDS             17925..19175
                     /gene="icd"
                     /locus_tag="OQN02_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1643532.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5016 a         4728 c         5158 g         4360 t
ORIGIN      
        1 ataaagaaca acacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
       61 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
      121 catggtaacg attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga
      181 atatgattgc gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc
      241 gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
      301 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
      361 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg
      421 cacgcgaaac aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg
      481 tggcttgggc caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa
      541 aaacgcgtaa ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt
      601 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
      661 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
      721 cttaccatgc cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg
      781 cagctgcttg ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac
      841 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
      901 gcgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
      961 gcgttaaaag ttattgccga acccatgggc gagccgtccg gcgatttgct gccgttcgcc
     1021 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
     1081 accacgcaca acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac
     1141 gcgctaaact ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg
     1201 cgtatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
     1261 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
     1321 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
     1381 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
     1441 ggaacgggca tcaagaaaat cacgggcggt gaccccgcgg aaattaaccc gaaatacgaa
     1501 aagcgtttca cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata
     1561 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
     1621 gttagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
     1681 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
     1741 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
     1801 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
     1861 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
     1921 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc
     1981 gcgaaagttt acggacatga atatattacg cggaaagtta agggagtaac gcagactaac
     2041 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
     2101 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agatagcctt
     2161 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
     2221 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta
     2281 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
     2341 ttaaacgagg gggtaaaaat aaatatgcac acttcaggga aatttaataa atcattcaaa
     2401 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
     2461 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
     2521 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
     2581 gagattgccg aagcggaaca ggtaacaaca atcaacgtta aaaccggaga ggtggaatca
     2641 tgagcgaacc aaaatttggt gaaaaatttt acaagcacaa cggacggatc acgattctgc
     2701 aaataagcgc agctacacca ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg
     2761 catcatgggc gctttgtgtg cttagttacc ctgaccgcga tgtgtatcag gatattctgc
     2821 ctgtgatatc cactgacaaa ggcatgaaac ccgtagacat caaaaacatg gggtttcagt
     2881 gcgtaatgct cactgaaaaa atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc
     2941 actgaggtgg caatatgaca gcacaaattg cagcttacgg gcggctggtg ggcgacccgc
     3001 tggtaaaaca gagcagcaag ggcacaccgg agccgccaac ggagaactac gcagacctga
     3061 gaaatgcagt aaatcgcact tacgcagaca tggggctgag caaattagca gagtaggaat
     3121 atcagaacca caccacgcag ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta
     3181 tggagcacta ctggcttttc gtcacggtcc ggcatagtta ctatctgaaa caaacagaca
     3241 caacagagga aaacgcaatg ccgatgaaat ttgatgagat acttaaacag cgtgataaat
     3301 accatgctga caacatggag acgatgaata tcactgatta ccgcgcattt ctggaaacgg
     3361 gggcgctgat tgaaaaggat catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc
     3421 tggcagtaaa tcctgaacag acagatgcat tgatagaatt tctgaaaggg atcagagact
     3481 gagcacccat acagcacata cagccggagc aatccggctt tttgttattt tttgtaaatt
     3541 atttgttcgt ggttgttcca cgttgttcac tgagcggatc ggcatatttt acccgaactg
     3601 aatcatgatt attctcgccc gtggtgccag gacgctgggg ccactttccc gcctgttaat
     3661 gtgctcgcca atattcatta ccaggcggga aaacgatcgg tgcgattact gatttcctta
     3721 tgaaaaacgg ttgagtgttt gccgcgtcct ggagttcctt acttaacccc aggacttttt
     3781 ttatgcagag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
     3841 acatgctgga gaacgctgaa aaagaaaacc gcagtcttaa cgatgctgag ggcacaaaat
     3901 ttgacgaatt acgcgctaaa tctgaatccc tcgataaaga catttcccgc cttgaagcca
     3961 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac cccgcagaac
     4021 tacgcaacta cattctgacc ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
     4081 gtggttatac cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat
     4141 ccaccatgcg tcgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
     4201 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca
     4261 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
     4321 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
     4381 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
     4441 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
     4501 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
     4561 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact
     4621 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattat atctggcgcg
     4681 accgtttaca gacgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
     4741 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
     4801 acgaaacagg tgttcgcacc agaccggaca acctgacaga accaggattc atcaaaattt
     4861 tcacgcaaaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggcac
     4921 tgccggaaga cgacgattaa cagcatacag aaggggctaa aaagcccctt tagtgtttta
     4981 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggag
     5041 ccggtacgct gacgggctac gttgttcgct gggataagct ttcagaactg ttatggggtg
     5101 agttttacga aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg
     5161 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
     5221 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
     5281 cagggcgcga cgttatcgaa ctggttaagc gcggggacat ctcggggatg agctttggct
     5341 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
     5401 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
     5461 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
     5521 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag
     5581 cagcgcagca tgacgcttga tgaatttatg gcgctggccg gcacatcgaa cacgggggcg
     5641 ggtgagtacg tatcatctgg gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg
     5701 gtcatctctg acgcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
     5761 gggaaggagg cccgcgaatg gcttgattct catccggtcg atcacatcct caacgagcgc
     5821 ccgaacgcgt ggcagacacc ctaccagttt aagcgaatga tgatccgcta ctgcctgtta
     5881 agtggtaatg cttatgcggt tattcagtgg gggcgtgatg gttttccggc ggctttacat
     5941 ccttacccgc cgcagtcggt gaacgtggag cagacaggtg atcataactg gcgctactgc
     6001 atcactgacg cctacaccgg aaacacccgc aactatttac cgtgggaggt tctccacctt
     6061 cgttactcca cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg
     6121 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
     6181 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
     6241 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
     6301 gggatgagct atcagcagct gggcatgagt aatcaggatg ccgaatggct ggcctcccgt
     6361 cgcttcacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
     6421 tacagcaaca gcacctacag caacttcagc gaggcaagcc gcgcatttct caccatgacg
     6481 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccttgct ggtggcctcg
     6541 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
     6601 cctggcgaac gctttgccac ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac
     6661 gaagcccgcg aacgtgaagg gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca
     6721 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
     6781 gtgacaggat aacgattcag accctgaaac agaccaggga tataaccggc gaaatactcg
     6841 aaacgtggga ggacggtcac acactctggg caagcgtgaa catggtcagc agcaaggagg
     6901 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
     6961 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
     7021 tgaatatcat cgggcagccg ctgccggatg ccgccaggac acgccttgaa attctttgtc
     7081 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
     7141 ccgcatcgat ggcgacgatg aagaccagtt aatcagcgga tacattgccg catcgcttga
     7201 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggcgttca cgccagccat
     7261 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
     7321 taacgcaaaa acacgcgtac ccatgacgat aggcgcactc tggacggctt accgtgatgt
     7381 gggggggtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
     7441 cgcgttaaat ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
     7501 agacgaggac accgccgcgc gcgtggatat tccagacagt gggacaaata ccgcgccatg
     7561 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
     7621 gtggtggtgg atcacattat cccgattaat ggcggtgatg atgttctctt ctggcccgaa
     7681 tggaaccatc aaccattgtg ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg
     7741 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac
     7801 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
     7861 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
     7921 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
     7981 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
     8041 ccgaccccgc ctacctggat gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc
     8101 ttgcggaaca gggagacctg acccccgccg actggagcaa tctggaactg tattgcgtca
     8161 actactccat ttaccggaaa gccgttgcag accttgcggc gcgcgggttc agcattgtta
     8221 acagtcaggg cggcgagagc agaaaccccg cattaagcgc aaaatccgac gctgaaagag
     8281 tgatgataaa aatggcctcc ttgctcggtt ttgacccgat aagccgccgt aaaaatccac
     8341 cgtaaacaga agaagaggac gagcttgacc gcctggaata agtacgcaga agacgtaaaa
     8401 acgggcaaaa ttccggcctg taaacggtta aaacaggctg ttaaacggta cttttcggac
     8461 cttaaaagcc ccctttacac gttcgatcgt gaggttgtgg agcggtttat tgccttttcc
     8521 agggtgtgcc cgcacgtaaa aggaccgatg cgtggcagac ccattgagct ggagccgtgg
     8581 cagcagttcg cctttgcgtg catcctcggc tttaaggtta aggccaccgg acggcgcaaa
     8641 tacacgagcg cctttatcga agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt
     8701 ctggctaact ggtttctgat tatggagaac ggtcagcagg atatctacac cgcagcggtg
     8761 agccgtgacc aggcgcggat cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga
     8821 ccgttacgca ggcgggtgaa tattcaggcg cataaggtga tacacccgaa aagcaacagc
     8881 ctgttaaagc cgctggcagc aaaagcggca accatcgagg ggacaaaccc cagtctttcc
     8941 atcgtggacg aataccacct acaccccgat aacggcgttt attccgcgct tgagctgggg
     9001 atgggcgcac gtcccgaagg gctgttattt gccattacca catcgggcag taacgtcgtt
     9061 tcagcctgta aacagcacta tgattattgc tgccagatcc tggacggcga agaggtcaac
     9121 gattcaattt ttgtactgat ttacgaactg gacgacgaaa acgaggttga tgatccggcg
     9181 atgtggataa aggcgaaccc taatattgat gtttccgtgg atcgtgaaaa actggcctca
     9241 accatccaga aagcgcgggg tattccgtcg cagtgggtgg agatgatgac aaagcgattt
     9301 aatatctggt gtcaggggac taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc
     9361 ggaacgttca cggaggaaga tttacacggt caggagtgtt acgcggggct ggacttatca
     9421 tcaaccagcg atatttccag cgtatgctat gcctttccgg tcggtaaaac cattatgctg
     9481 atttcccgtc attatctgcc ggagttccag ctacagaacc ccgccaataa aaaccgcgct
     9541 gtctatcgcc agtgggctaa agcgggctgg atacgcacaa cgccaggtga ctgcattgat
     9601 tacgaccgga tcagagatga catcatgcag gatgcggaga aatttaatat caggctggtg
     9661 ggctttgata cgtggaacgc cacgcatctc aggacgcaat tacagggggc tggttttgag
     9721 gtggagccgt tcccgcaaac ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt
     9781 tttgttaacc gcagggtgat tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt
     9841 aacgtcgtga tgcagagtga cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg
     9901 aacaagatag acccgagcgt agcggcgctg atggcgtttg gtacattcca ggcagagcat
     9961 gaggattttg cttttgatat aagcgacagc caccgccaga aactggaaga atttagcggg
    10021 gtgtaatgag gtcagcagca tgacagaagc cgaaatactg cgattaatcc gccgcgtctc
    10081 tggaatcagc cagcaggctg acgaacagac cacgcagccg gacagcgtga cagccgaaaa
    10141 ttatgcgcgt gttgttgctg aggtgatgcg ccgtgatggt atccagctta atgatgcgga
    10201 tatgcgcgac atacggatcc gcgttctgga aatgctggcc tacaatcgcc gcgttgaact
    10261 gtatcgggag aaggagaaaa tcacgtacca ctggaagaag ccggagcggt tacggcggta
    10321 actcactgag attaaagcaa agcgcaaaat tgcgctggct acatccctca catctgagga
    10381 ctgttaacca gcaggccgga atatattcag aaattacgaa attttcgcag tttaattact
    10441 cgtcagctta atcgctaacc cgctctgata ttttcggaaa cctcaatttg aggaagtcgg
    10501 cgaggtaact gtttgatatt cccgatagcg caaaaatgcg cggtcgggca gaatgaccac
    10561 cacctcaatc gtgacggaga gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa
    10621 ccgttcatta aaaatcgacg tgattttgtg ctttccggat gacgtggcca acatcatttt
    10681 ttagcgaaaa attctgatta aggtcactgg ctgaacggtc ataatgacca cgcagaccat
    10741 cgaggcaacc atagcgatca tcgttacggt tggagctttt ccgccattgg tggaaaaggt
    10801 gcacatctgg atcatcatta cggtgatcaa gggtatgtaa aacccaccag cctgattaaa
    10861 ggttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    10921 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    10981 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    11041 tttgcgattt ttggcacttt ttcaatgaaa attgtttgat cgaatcaatg acttgatgta
    11101 gtggtaaaag gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    11161 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    11221 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    11281 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11341 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11401 tatttacagg cattatccag cacgttgccc atcacctcga caaaatcgtt ctgctcaccg
    11461 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11521 ttgttcagcg ctgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
    11581 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11641 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ttcactacgc
    11701 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11761 gtgagcgtcg tacggtattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11821 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11881 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11941 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    12001 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    12061 aattttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    12121 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    12181 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    12241 cagtcaggct ggatcatttt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12301 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12361 tcgacatgca acttattgtt ttcccactgc gcaagggtat agaacagatt gctctcgcca
    12421 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    12481 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    12541 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacaggtagc
    12601 cctggccgcg aacggtggta atgacttctt gcggatattg tgcctgaatt tttttgcgca
    12661 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    12721 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12781 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    12841 agagatcaac ctgaaacggg gggagcgaaa tgacctgtga agccagaccg ctattacgcc
    12901 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12961 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    13021 ccagaatcgg cagtgaaaca tcgttgctac gccagcggcg aatcagtgac agaccgtcct
    13081 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    13141 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    13201 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    13261 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgt ttatgggggt aaacattaaa
    13321 taaaccagcg gggaggggag gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca
    13381 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatcg
    13441 tgatagctcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    13501 ctggcaacgc cagaccatcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13561 gagtaagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13621 ggattggttc tgccagcact tcccagttgt gatccagctc atccagcaga tggtcacggt
    13681 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13741 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13801 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    13861 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13921 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13981 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    14041 gttcaatctg ggtagtgtac gggttccatt gaatacccag cgaggtgacg aactcttcgc
    14101 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    14161 cgccgttgat tttgccgagg atctccacct ggttaagctg gcggtgctgg cgctccatac
    14221 ggtaggcgac gtttgccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    14281 tacgagacag cagcgggata tcgcggtact gaacggcgag atctttaatg ccatcaatca
    14341 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14401 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14461 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14521 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa
    14581 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14641 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14701 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14761 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14821 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgtaga cgtccgccgc
    14881 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg
    14941 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    15001 gcgggctgat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    15061 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    15121 cgcctttcgc tgaggagagt ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta
    15181 attcggcgtt taagccctgg caactgctgg cattgagcac gccgagcagg gtttccagcc
    15241 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    15301 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    15361 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15421 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15481 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgttat agaagacggc
    15541 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc
    15601 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    15661 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcaaccc
    15721 gacagacatc agccgcgggt gttcatggcc ctgagcgaca accagaatgt tgttttcgac
    15781 gtctttgtcc accacatacc acggttcttc ggtgccttct ttggtgccac cgatacccag
    15841 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15901 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15961 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    16021 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgcctaccg ggaacaggct
    16081 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    16141 cagaccacgc agcaggcggc tcttgccatc gacatcggcc cgacgtacgt aatgaccggt
    16201 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    16261 ctccttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16321 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16381 gagcttgtcg cagaccgcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    16441 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16501 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16561 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16621 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16681 ggataacgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16741 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16801 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16861 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16921 ccggtttctt cccacagttc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16981 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    17041 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct
    17101 taatctgtca cttctcgcca ttcgccattg gcaagattat ccaaagagta atctcccatc
    17161 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    17221 cgattacgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttgcgttca
    17281 cgaattggtg gattccgcgg ccataaccac gcgggttcgt caaccagctc cgcgcctgcg
    17341 ggcagggtag ggccatcatt taaggttacg ccattgcgca aggcttcaag tgcgtcttgt
    17401 gtgggaatac cttccacctg cacataatag atttttccgg tgcgtttacc cggctgggtt
    17461 aaacgcgcct gcaacgcgcc gttattggtc agcaccagca acccttcgct atcgcggtca
    17521 aggcgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17581 gtttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaagaggat cacacgcgtg
    17641 ggctggtttt catgtttacg cctggtagaa cgttgtgagc tgaatcgctt aacctggtga
    17701 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17761 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag tcaattacaa
    17821 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17881 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17941 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    18001 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    18061 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    18121 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    18181 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    18241 cggttggtgg cggtattcgt tctctcaacg ttgccctgcg tcaggaactg gatctctaca
    18301 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18361 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18421 aagctgactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18481 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18541 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18601 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18661 accagctggc gcgtgaagaa tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18721 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18781 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18841 gtgactacat ttctgacgct ctggcagcgc aggttggcgg tatcggtatc gcccctggag
    18901 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatacg
    18961 ccggtcagga caaagtaaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    19021 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    19081 ccaagaccgt aacctatgac ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt
    19141 cagagtttgg tgatgcgatc atcaagaata tgtaatcact acatgtgtta aatatttaaa
    19201 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atatcgagca
    19261 ta
//
LOCUS       DAJVRL010000080        17505 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVRL010000080 DAJVRL010000000
VERSION     DAJVRL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.080"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            15..176
                     /gene="relE"
                     /locus_tag="OQN02_002808"
                     /pseudo
     CDS             15..176
                     /gene="relE"
                     /locus_tag="OQN02_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
     gene            266..856
                     /locus_tag="OQN02_002809"
     CDS             266..856
                     /locus_tag="OQN02_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCS1643533.1"
                     /translation="MKIYVIKIAVHGVSPMVWRRLRIAADTSLAALHFIFQIVQGWGD
                     DHLHQFHIYGKDYGISYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIR
                     VEAIYENSTLKAPFCISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADD
                     LDAVRFNRHKINRQLSRLDLASPVLEPEVIWLGRRR"
     gene            993..1565
                     /locus_tag="OQN02_002810"
     CDS             993..1565
                     /locus_tag="OQN02_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1643534.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            1602..2993
                     /locus_tag="OQN02_002811"
     CDS             1602..2993
                     /locus_tag="OQN02_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HCS1643535.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            3038..3454
                     /locus_tag="OQN02_002812"
     CDS             3038..3454
                     /locus_tag="OQN02_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1643536.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(3773..4429)
                     /locus_tag="OQN02_002813"
     CDS             complement(3773..4429)
                     /locus_tag="OQN02_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HCS1643537.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            4824..5839
                     /locus_tag="OQN02_002814"
                     /pseudo
     CDS             4824..5839
                     /locus_tag="OQN02_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5882..8887)
                     /locus_tag="OQN02_002815"
     CDS             complement(5882..8887)
                     /locus_tag="OQN02_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HCS1643538.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            9051..9608
                     /locus_tag="OQN02_002816"
     CDS             9051..9608
                     /locus_tag="OQN02_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1643539.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            9791..10651
                     /locus_tag="OQN02_002817"
     CDS             9791..10651
                     /locus_tag="OQN02_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HCS1643540.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            10861..11265
                     /locus_tag="OQN02_002818"
                     /pseudo
     CDS             10861..11265
                     /locus_tag="OQN02_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(11372..12208)
                     /locus_tag="OQN02_002819"
     CDS             complement(11372..12208)
                     /locus_tag="OQN02_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1643541.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(12208..13011)
                     /locus_tag="OQN02_002820"
                     /note="aph(3'')-Ib"
     CDS             complement(12208..13011)
                     /locus_tag="OQN02_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1643542.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(13072..13887)
                     /gene="sul2"
                     /locus_tag="OQN02_002821"
     CDS             complement(13072..13887)
                     /gene="sul2"
                     /locus_tag="OQN02_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1643543.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            14217..14393
                     /locus_tag="OQN02_002822"
     CDS             14217..14393
                     /locus_tag="OQN02_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCS1643544.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(14575..15579)
                     /locus_tag="OQN02_002823"
     CDS             complement(14575..15579)
                     /locus_tag="OQN02_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HCS1643545.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(15658..17424)
                     /locus_tag="OQN02_002824"
                     /pseudo
     CDS             complement(15658..17424)
                     /locus_tag="OQN02_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT         4223 a         4471 c         4710 g         4101 t
ORIGIN      
        1 ctggatgaac tctgatggcg tattttctgg attttgacga gcgggcacta aaggaatggc
       61 gaaagctggg ctcgacggta cgtgaacagt tgaaaaagaa gctggttgaa gtacttgagt
      121 caccccggat tgaagcaaac aagctccgtg gtatgcctga ttgttacaag atggagagca
      181 tgaaaaacct cggggcagcc atcgaatgat gccatctaca ggggttaaaa taaccaccat
      241 catttcataa caatacctgc tgactatgaa gatctacgtc atcaaaatag cagttcatgg
      301 agtcagtccg atggtctggc gtcggctgag aattgctgcc gacacgtcac tggccgcgct
      361 tcacttcatt ttccagatag tgcagggctg gggggacgac catctccatc aatttcacat
      421 ttatggcaaa gattacggta tctcctacga aggaggcatc ggttttgtcg ataatccgtt
      481 ccgggtcgtg attgatgatt ttgcttttga tgctggcgat cgctttacct atgaatacaa
      541 tttctttgag cactggcttc atgacattcg tgttgaggcc atttatgaaa actctacgct
      601 gaaagcgccg ttttgtataa gcggccatgg tatgcccgga gccacagctg cggatgagtt
      661 cgataaaacc ctggcgtttc ttgaagctat cgttaatgcg gatgatgaaa caacggtcgg
      721 cgagatccgc ccttttgctg acgacctgga tgctgtcagg ttcaaccgcc acaaaatcaa
      781 ccggcaactg agcaggctcg accttgcatc tccggtactg gagcctgaag ttatctggct
      841 gggccgtcgt cgctgactga tgtctataaa ggtatacgtt ttcagcatgc ctctcagatc
      901 cccgaaaaaa tgtctataaa cctccctaaa tcagtgttta tggattattc tttaaatctc
      961 tatgatgttt aaagaccttc cggaggtttt gcgtgcaata tgcctacatc agggtaagct
     1021 cggccgatca gaatacggcc cgtcaggaag aggcattgtc aaaggcgggt tttcagcctg
     1081 ataaaatttg tgttgagcat gccagcgcga aggacacaaa ccgcccgggg ttacaggaac
     1141 tcctggggca attacgccct ggtgatacgc ttctggttca ttccatcgat cgcctttgcc
     1201 ggaatatgtc ggatatgtgc gctgtcacca cccggcttcg tgaccagggc gttaccctta
     1261 tttttctgaa agagcagctg acgttcagcg ccggcacaaa caatccgatg caggaactgc
     1321 agctgcacat gatgtcggca ttcagccagt ttgaacgagc cttactgaag gaaaggcagg
     1381 ccgacggcat cgccgcgaaa aaagcacggg gtgaaaaaac agggcgcccc ggcgccgata
     1441 tcaaaaagat ccaggaaatt gacgcactca gaagcagagg ggtcaggctc aggatcgcct
     1501 gcgaccatgc gggccttggc gtttccacat attataagct tcgtcaccag atgaacaacc
     1561 agtagccggg agatagtcat ccaccacagc aggaggcaat gatgcaactg acgctccaga
     1621 tagtcatcac cgatgaatca ggctccagtc gaacggagga actgatgaca atccagaaat
     1681 caggggagac ccggaacgac atcggattat cggtgtcaga atccaagctg ttgctgaata
     1741 cggtccagca gtcggtggtc cagctgcagg cagacgaata tactcaacac catatccggt
     1801 gccctcactg ccttgctgcg cgcagaatca aaggcaaaca gaaaatacgg taccggacgc
     1861 tgtttggcgt tattccggta tccgggcttc gggtgtaccg gtgccgttgt gaagagagtg
     1921 atacaaagac agtcagtttg ctcagcgact gggccggcga ttattctcac ccggcactga
     1981 aatatattga aacccgctgg gcctcgatga tctcctatga aatgacgact cgtctgctga
     2041 aagatattct gccggtgggc cacagcctga atgcctcaac ggtgaggaat catttatgcc
     2101 aggtggcgca gcgtcttgac gctgaggctg aagctcattc aggttttctt tccggctgcc
     2161 cccgcgactg ggggaacctg cccaggccag gaaaaccgct tgtggtgggt atagatggtg
     2221 gttatgttcg cgatcgggat gacaaaaagc gtaattttga aattattgcc gggaagtctt
     2281 tttccgttgg tgcgcctgct gacacccgcc gcttcggttt tgttcagaag gatgactgtc
     2341 atccggaacg caggctcatg acccaccttt cagcgcaggg aatgcaggcc aaccagcaga
     2401 tattttttct gtccgacggc gcggataatc tcagggacct ccagttcggt atgtaccctg
     2461 agtcaacgca tgtgctggac tggtttcata tcaccatgag gctgaaggtg ctcatgcaat
     2521 atgcccgggg actgctggta tcagatccag aggcggggag taaagttctg gcactgctgg
     2581 aaagcattaa acgatatctc tggcatggca atgttgtcgc tgcgctggaa catattgata
     2641 attgcgttat gtattgcgat gaccctgaac tcagctatcc tagcctgaaa tccctgcaaa
     2701 aacatctgga tgaaatgtat acctacatcc ggaataataa gatgatgatc ccaaactacg
     2761 gcgaaatgcg tcggtacgga gagccggtat caaccgcgtt tgtggaatcc acgatcaatg
     2821 aagtgatcgc cagacgaatg gccaaaaagc agcagatgca gtggagcaga aaaggggctc
     2881 attatttgtt acagacccgg accgccgttt tgaataacga actgcaggat aaatttgtct
     2941 gctggtatcc gggttttcaa agtgacggga agggaccagc gatggcagca tagctcacgc
     3001 tgccccaata ttttttatgc tctcggtcgt atggaagctg gaccgcctcg gtcgtgatct
     3061 tcgtcatctc attaatacag tgcacgacct gaccgcgagg gggaccggtc tgaaagtgct
     3121 gaccggtcac ggggcgacta tcgacacaac aacggccgct ggcaagcttg tcttcggtat
     3181 ctttgccgca ctggcggagt ttgagcgcga actgattgct gaaagaacga ctgcaggtct
     3241 ggcatcagca agagcccgtg ggcggaacgg cggccgacct tataaaatga cgccagtcaa
     3301 actgcgactg gcaatggcat caatgggaca atcagaaaca aaggtcagta cgctatgcca
     3361 ggaacttggg ataacccggc aaactttgta caggcatatt tcccctgttg gtcaactgcg
     3421 ggcggacggg atcaagctgc tcaaccgtgg ataatttgaa cggacggaaa aatctggggt
     3481 tccggcgtag tacccctatc acctccacat ttttgcaaca gtgccttata aatggcagcc
     3541 ttatgtttac ttgccaagat gggctaccat atgatgtttt atactgagca atattaatat
     3601 tatctttaat ggaaaaatta agcccactaa gtgacccact gctatatggc tctattgtga
     3661 agacgcctga gggtaaaaat ttatacatgg ttgtccctat gtctattatt gcaaggttga
     3721 caatattatt cgttttataa atttgagcgt ttaaaatcac acatcaaagc aattagtcag
     3781 gataaacaac aatacccagt gcttcgagaa tcagttcttg ctgccaggct gcaattttga
     3841 tacctgatgt atcgactttg cggggatcta aaccgtcgag ttcggcgtgg cataaattgg
     3901 caccctgtag gctaaattgc ccccagacat cttcggaaaa aacacctcga cttaagtctg
     3961 actctttcag tgatgcaccc gctaggttcg ttcctatcca gcgattttca aacaactcac
     4021 atttttctaa acaaaccctc tccatattgg cataggaaag attacatcca gaaataaatg
     4081 ctgagcaaaa gtacatacga ttactcactt gatgggcaaa gtttgttcgg gaaaagttgg
     4141 caccttttaa atcacacgca cggaactcta taccgtagca attggcatta ctgaagtttg
     4201 ccatcgcaag ttggcattgt tggaaacttg catcacgaag atctgcgaca tcaaagtggc
     4261 agccttcgat atcaccctgt tcaatgaact tgcagttgac gaatgtcgta tcacgcaagt
     4321 tagcacgtcg aaagtcgctg cgaatgaatg tgcaagcggt gaaggtgaga tcacttaagt
     4381 ctttatgtga aaagttgtgg tgccgatatg tatgattgta ggtttccatg agtactcctt
     4441 gttgaaagga gggctagact accattatac atatgccaag acattaaaat aaaagcctgt
     4501 tacatcaaca cattacaacc cctcaattta cctgttttaa gtggtcaaat tggcgcgtag
     4561 agcgcctatc gctcttgcat tggcacgcgg ccctgttgaa aaaagttatt cgctttagat
     4621 agggttagga gagccgtgct tcgccggtaa aaaatcggac tatccgacgc agggcggact
     4681 catcggcgga gaagcaaact ttgactatat ctccctacac ttggaagagg gctagactgg
     4741 ccccatagat caccagacac ttcgactctc ttaaaataag aggtagtctt ggaactatgt
     4801 ttaataccag tcagaacgat aagatggttg aagtgttatc aggacctgag cggcgtcggc
     4861 gtcgtacacc gcaggaaaaa attgccatta ttcagcagac tatggagccc ggtatgaccg
     4921 tgtctcatgt cgcgcgcctg catggcatca atgcaaacca gatattcaaa tggcgcaagc
     4981 aatattaaga cggttcactg acggctgttg catcgggcga ggaagtggtg ccggcctccg
     5041 aactcgctgc cgccaataag caaattcgcg aactccagcg ccttctgggc aaaaagacga
     5101 tggaagccga gatactcaaa gaggccgtgg agttcggccg ggcaaaaaaa tggattgcgc
     5161 atgcgccctc gttgcctggg aacgacgaat aagcgacgtt tgcagaagtc ttggcgtgtc
     5221 gcgtgcgcag ttgagcattc gggttcaccg gccatctgac tgacaggatc gcagacggca
     5281 gccccgttct gacgatacca ccgtattgtc ccggataaac atggcggtgg ctgacctgcc
     5341 gacgtatggt tatcgccgtg tgtgggcgct gctgcgacgg gagtcggagc gggacggcct
     5401 gcctgtggtt aatgcaaagc gggtgtatcg cattatgaga acgcataatc tgcttcttga
     5461 acgtaaaccc gctgatcctt gccgtaagcg ggcccataag gggcgcgtag cagtagcgga
     5521 aagtaaccgg cgctggtgct cagatggctt tgagttccgc tgtgataatg gcgaaaagct
     5581 gcgggtgacc ttcgcgcagg actgctgcga cagggagatc attgactggg cagcgagcac
     5641 cggaggctat gacaaagaaa cggtgcagga tgtcatgctg ggtgcggtgg aaaaacgctt
     5701 cggaaggcag ttgccaacag tgccggcgtt cgcccggttg ctgggactgg agccttgtac
     5761 gacagcagtg cgaagcccgg aaagcaatgg catagcagaa agcttcgtga tgacgataaa
     5821 acgggactac atcagcatca ggattttagg ggtctgacgc tcagtggaac gaaaactcac
     5881 gttaagcaac gttttctgcc tctgacgcct cttttaatgg tctcagatgt cctttggtca
     5941 ccagttctgc cagcgtgaag gaataatggc cgagcatatt gatatgtccg tggcaaagcg
     6001 gggagaggcg tgcgatatct tcatcattca gtgtttcacc ctgcgcccgg agatgatcca
     6061 gggctgcctg catataaata gtgttccata acacgacggc gttagtgacc agccccagtg
     6121 tgcccagttg atcttcctga ccgtcggtat atcgttttct tatctcacct ttttgaccgt
     6181 gacagatggc tctggcaacg gcatggcgac tttctccccg attaagctgg gtcagaatgc
     6241 gccggcggta atcttcatca tcaatataat taagcagata cagcgttttg ttgatgcgcc
     6301 ccacttcaat gattgcctga gtcagtccgg aaggacgttc acttttcagc aatgaacgga
     6361 ccagcactga aacctgtact ttgcccagct tcagggagcc agcggtccgg atcatttcgt
     6421 cccactgaag gactattttt cggggatctg attgccctct ggcaatatca ttcagcacgc
     6481 catagtcggc atcatggtcc attcgccaga aaaccgaagc accggcatca gccaggcgtg
     6541 gagaaaactg gtatcccagc agccagaaaa ggccaaagac aagttcgctg gcacctgctg
     6601 tatcggtcat aatttcggtt ggattcagcc cggtctcctg ttccagaaga ccttccagca
     6661 caaagataga gtccctcagc gtccccggta taacgatgcc atgaaagccg gaatactgat
     6721 cggacacaaa gttgtaccag gtgatccctc tgttattacc aaagtatttg cggttcggtc
     6781 cggcattgat tgttctgact ggcgtaacaa agcgcattcc atctgcagat gccacttctc
     6841 ctccacccca tatctgtgcc agtggcagcg ttgcctgaaa atcaaccagt ctggcattag
     6901 cgctggtgat agtttcagcc cgcagatagt tcgcttttgt ccagttcagc cggtgtcggg
     6961 tcagtgcagg aacatttgat ctgatcagtg gttccagacc gatattgcag gcttcagcca
     7021 tcagcacggc gctgatgctg acgggcagat catcaactct ggcactggct tcactagcat
     7081 ggaaaaactc atcagcaaat ccggtatggg cgttaatttc gagcagcaac tccgttaaat
     7141 ccaccggagg gagtagatca ctgatcattt tgctcagtcg tttcagactg tccggctcat
     7201 caagactggc gaggggagaa attgtcaacc ggggcttcgg gccagaaaca tcgagttcga
     7261 cagcctcatt ttcgcaaaga cgtgcagcaa cctgtctgta acgactatca agctgatgac
     7321 ccagagattt tattgcttcc tgcgggtctg tcgggtgccc caaagaacga taaaccttaa
     7381 tccggtttgc ctgccagtca gcaccctgta gtaatcttgc acgaggatct ccccaccggt
     7441 tactgccggt aacgtagaca tccctccgcc tcagactatc ctgcagttta ctgagaaagc
     7501 agagcgtgta tcccctgcgg gtgatatgtt tttccttgtt aatcaccagc cgtttccatg
     7561 accgactgat aatttccgtt ggtgcgtcgt caaaaaactg ccgccgtgag ctgaactccc
     7621 ggctgaggta gtcacaggca ttcagagtgg taaccccggc aggtgcggat gaaaatttaa
     7681 cggtattcag cagatggggc aggaaacgac gaacgcgccc gtactgctcc accatttctt
     7741 catgaaaatt atcgtctgag ggccgggcaa tttcacggac aagcgtgatg atttcagcca
     7801 gcttttgcct tgggatgtag ctgaacacct cagcacgaat cgattcgtcc ggtgtttctt
     7861 ctttcagcag gtacgaacat gcgctggcga gcgccaatgc agatttatcc agatccttca
     7921 gcgagcggag ccgttttttc tgcccaatct ttctggcgtc acggatgata acggccagca
     7981 tggcgtccag aacgtccaat gcatcatcca gcgccagcgt ttcccatgca aggacaaagg
     8041 caaccagaac cgccatcctt ttctgcggtg acatcctggc aatattgaac accgaagtca
     8101 taccagcata acgtgcgaga tttttcaggc gcacagccgg gagtgtactc aggttttcag
     8161 catgcaggcc aaaatcgttc agagttttcc agcgttcaat tgcttcatta aacgccggac
     8221 cactgatggt cacagggccc tttttcagtg attccagtaa agacaggcgg ctgcaatcag
     8281 ttggccccag cagcatctcc agctgtgaac gctgttcggc tgacggtatc agtgccagtt
     8341 tgttccacag gcgcaacgtc gccttttccc ttacctctga aatcaaccgg gtcagcgtag
     8401 tggctccggg gagaataata cgatgttgca taagccaccc tgtcgccaga tcgaaaagca
     8461 ggccaggacg ttcgttgctt atccagctcc gggtatataa aagacgggta aggcgaaatg
     8521 tccagggcca ggcaaattca cgatactgat agtgctgacg tatcagcgct gcatgctcac
     8581 ggcgggtatt ttccctctga ccgtattctg caagaacggt gatatcacga atcccgagct
     8641 gtctggcggt aaaatgccgg acgccggaag gaatatgatt catatcggtg aggaaggtgc
     8701 ccagaaaacg gacacatcca atttgcaggg caatgcccag acggttgtga tcacctctgc
     8761 tttttccgat aaattccttg tctgcttcat caaggtgaaa atatcgtgcc agctgaagct
     8821 catccggttc accggtgaat ctgccatagc tttcagtctg ctcagtggtc agaaagtcaa
     8881 cgggcatatc ggcctccctg cctgacgggc atttagtaac atttttccaa ccgtacgaaa
     8941 tgttataaat tatcggacat cgtaaaactg ttacattaat atgtctatta aatcgtaaat
     9001 ttgtaataat agacatgagt tgtccgatat tcgatttaag gtacattttt atgcgacttt
     9061 ttggttacgc tcgggtctca accagtcagc agtctcttga tcttcaggtc agagcactca
     9121 aagacgcagg tgtgaaagca aaccgtatat ttaccgataa ggcatccggc agttcaacag
     9181 accgggaagg gctggatttg ctgaggatga aggtggagga aggtgatgtc attctggtta
     9241 agaagctcga ccgtcttggc cgcgacactg ccgatatgat ccaactgata aaggaatttg
     9301 acgctcaggg cgtggcagtc cggttcattg atgacgggat cagtaccgac ggtgatatgg
     9361 ggcaaatggt ggtcaccatc ctgtcggctg tggcacaggc tgaacgccgg aggatcctag
     9421 aacgcacgaa tgagggccga caggaagcaa agctgaaagg aatcaaattt ggccgcaggc
     9481 gtaccgtgga caggaacgtc gtgctgacgc ttcatcagaa gggcactggt gcaacggaaa
     9541 ttgctcatca gctcagtatt gcccgctcca cggtttataa aattcttgaa gacgaaaggg
     9601 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     9661 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9721 ttcaaatatg tatccgctca tgagacaata accctggtaa atgcttcaat aatattgaaa
     9781 aaggaagagt atgagtattc aacattttcg tgtcgccctt attccctttt ttgcggcatt
     9841 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9901 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9961 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggtgc
    10021 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    10081 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    10141 aagagaatta tgcagtgctg ccataaccat gagtgataac actgctgcca acttacttct
    10201 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    10261 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    10321 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    10381 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    10441 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    10501 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10561 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10621 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10681 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10741 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccta
    10801 aaagaacttt ccgctaagcg atagactgta tgtaaacaca gtattgcaag gacgcggaac
    10861 atgcctcatg tggcggccag gacggccagc cgggatcggg atactggtcg ttaccagagc
    10921 caccgacccg agcaaaccct tctctatcag atcgttgacg agtattaccc ggcattcgct
    10981 gcgcttatgg cagagcaggg aaaggaattg ccgggctatg tgcaacggga atttgaagaa
    11041 tttctccaat gcgggcggct ggagcatggc tttctacggg ttcgctgcga gtcttgccac
    11101 gccgagcacc tggtcgcttt cagctgtaag cgtcgcggtt tctgcccgag ctgtggggcg
    11161 cggcggatgg ccgaaagtgc cgccttgctg gttgatgaag tactgcctga acaacccatg
    11221 cgtcagtggg tgttgagctt cccgtttcag ctgcgtttcc tgtttggggt cgtttgcggg
    11281 aaggggcgga atcctacgct aaggctttgg ccagcgatat tctccggtga gattgatgtg
    11341 ttcccagggg ataggagaag tcgcttgata tctagtatga cgtctgtcgc acctgcttga
    11401 tcgcggccgc gatagctaga tcgcgttgct cctcttctcc atccgcgttc caagctgcgg
    11461 aaaggcaccc ataagcgtac gcctggtcga gcaggcgacg cggatcgacg tccagcgcac
    11521 gagagaatgc gtccgccatc tgtgcaatgc gtctaggatc gagacaaagg tcgtctctgt
    11581 cagccggatc gtagaacata ttggcggcgc caaagcccac ttcaccgacc agaccgacgg
    11641 gatctatcac cagccagccg cgactggaga acatgatgtt ttcatgatgc agatcgccat
    11701 gtagcccacg cagttccgag gcattgctca tcatttgatc ggctataatc gccgcgtgga
    11761 cgtagtcagt ttgacaacct gcgttttgat catcgcgcgc ccgctgaaac aaagctgcaa
    11821 agcgatcccg gatcgggaga agggcagaag gcaggggttc ctcagatgcg gcatacagct
    11881 tcgccattag ttccgctgca atttcggtcg cctggtagtc gccgtgctcg gcaacgatgt
    11941 gagagagcat tcgctccccg gcatattcga gcaacatcag attgttctca cgaccgagca
    12001 accggactgc tcccctccca ttgcgccata ccagatagtc ggccccgcgc agttcatcag
    12061 caatgtcttc tataggtttc aatcccttga cgattgcagg agtcccgtct ggcaatgaaa
    12121 ctttccaaac gaggctggaa aaggtgtccg caatgagaac aggttgcgaa acgtgccaat
    12181 gagcaggaaa aacaggcggc atgaacatca accccaagtc agagggtcca atcgcagata
    12241 gaaggcaagg cgttcgcggt cgggggcttc gatccccaat acattgaata ggacagcgaa
    12301 ggcgcgctct gcttcatctg gcgctgccca gttctcttcg gcgttagcaa tcatgagtgc
    12361 caaatcggca tagcgatctg ctgttccgag ccgcccaagg tcgatcagac ccgtgcattg
    12421 aagagtttta gggtccacca tgaagttcgg catgcaggga tcaccatggc aaacaaccat
    12481 atcggtgcgc tcttggtcga gccgcaccgg tagctctcgt tcgacacgag ccaaaagatc
    12541 gagctgcggc gtactcttgt cctcgtccgg taagaagtcg ggattgacgg cattgcggga
    12601 caccacatca acggcgcgtc cgaacattcg cgacagcctg cgctcaaacg gacattgatc
    12661 aaccgatagg ctgtgaacag cgccaagttg ctgccccatt gacggccacg ctttgagcaa
    12721 atccgctcca gacagatcag ccgccggtac tcccggaatt gccgttatca ccaagcatgc
    12781 accctcctgt tcctcctgcc agttgatgac ctcggggcaa gccacacctc gacctttgag
    12841 ccaaatgagg cggtcacgct ctccagcgag ctcaccgcgg cgggaagcag gtgcgatttt
    12901 cgcgaaggca tgcccgtcac cacgtcgaaa aacaaaatca ccagattctc cgcctctgac
    12961 aggcaaccag tcagaatgcg attcaccaaa aaaaatatta gttcgattca atggaggttc
    13021 cttcagtttt ctgatgaagc gcgaatatag agaaatatcc cgaatgtgca gttaacgaat
    13081 tcttgcggtt tctttcagcg ccgccaatac cgccagcccg tcgcgcaagg ggcgcggctc
    13141 gtgtgtgcgg atgaagtcag ctccacctgc ggcggcggca agctctgcag cgagtgtcgc
    13201 ggccccgaca tcccccggac cacggcctgt gagcgcgcgc agaaaggatt tgcgcgaaac
    13261 agacagaagc accggcaaat cgaagcgcag ccgcaattca tcgaaccgcg ccagcaccga
    13321 gagcgaggtt tcgggagcag cccccagaaa aaaccccatg ccgggatcaa ggacaaggcg
    13381 gttgcgtttg ataccggcac ccgtcagcgc cgcgatgcgc gcgtcaaaga acgccgcaat
    13441 gtgatccatg atgtcgccag cgggtgcctc gcgccgatct gcctgcccgt cttgcaccga
    13501 atgcataacg acgagtttgg cagatgattt cgccaattgc ggatagaacg cagcgtctgg
    13561 aaaaccgcga atatcattga gataggccac accacgcgac aaggcatagg cttgcgtcgc
    13621 gggttgataa ctgtcgagcg agacgggaat gccatctgcc ttgagcgcgt ccagcaccgg
    13681 cgcgatacgc gcgatttctg tgtcggacga aacaggcgcg gcgtcgggat tgctggatgc
    13741 cggaccgagg tcgatcacat ctgccccctc ggccatcagc ttacgcgcct gcgcaatggc
    13801 tgcgtctggc gccagatacc ggcctccatc ggagaaactg tccgaggtta tgttgacgat
    13861 gccgaaaatg atgagcgatt tattcatggg ggcttctata ataataataa tcgagcatga
    13921 gtctcatacg gatgctcggg tcgaaaggga atccccaggc gagtaacctg tttgcggtgc
    13981 tccattagct gcaggagcag aatagcatac atctggaagc aaagccagga aagcggccta
    14041 tggagctgtg cggcagcgct cagtaggcaa tttttcaaaa tattgttaag ccttttctga
    14101 gcatggtatt tttcatggta ttaccaatta gcaggaaaat aagccattga atataaaaga
    14161 taaaaatgtc ttgtttacaa tagagtgggt atcggagacg aagaaggaca aggtacatgc
    14221 tgaccgatac caagctgcgc aatctcaagc ccagggacaa actctacaaa gtgaatgacc
    14281 gggaaggtct ctatgtgggg gtcgcctcag aaaacggaat ctatggtcac tcccgttttt
    14341 gcaacaccga ttttgacgac aagttggctt gcttgaatct atccggcgtc tgaatgggat
    14401 tttattcccg cgcctcgatg agttccgcgc ctgatgaacc tccagaaaat atacggcttc
    14461 aatgagcctt tccgttttac aggttcctca acaggccggt gggccgttag tatcatcaat
    14521 atcagtattc gcaaaaccag atgaatgatt gtttaaactg gtgtatttct gcctttatgc
    14581 ttcgtaagtt tgctgtcgcg ccgtcagtgc ccaggctatt ctggccagct tgtttgccag
    14641 agcacaggtg acgacaaagt tgcttttccg acacaacaac tccctgaccc agtcggccaa
    14701 cttgccagac tggtgttcca gtttttgtat gaataccctg gcacactgaa ccaacaaagt
    14761 tcggatcttt ttgttgcccc gcttgctaat ccctaacaat gtcgtccgac ctcccgtgct
    14821 gtactgtcgg ggtaccagcc ctgttgccgc cgcaaagtca cggctgctgg cgtactgctt
    14881 cccgtcgcca atctcagttg aaatagtact ggcagtcagc gttccaacgc agggaatact
    14941 cagcaagcgc tgtccaacct catcttcgtc caactttcgt ttcaactgag attccagatc
    15001 tttaatctgc tcaacaagat agtgataatg ctgttgtaat ttcagcagta actggctgag
    15061 ataaagaggc aaactactgt cctcaagaag ggtactcagt cgactaataa cggcagcacc
    15121 tcgcggaacg ctgataccaa attccagcag aaaagcatgc atctgattag ttgttttcac
    15181 cttatcctga accagggatt cacggacacg atgcagagct cgcattgcct gctgagattc
    15241 ggttctgggc tgcacgaaac gcatagatgg acgtgatgct gcttcacaga tagcttcagc
    15301 atcaacgaag tcatttttgt tgcttttaac gaatgggcgg acaaattgcg gtgatatcag
    15361 ctttggaaaa tgccctaact cttccagctt gcgtgccata aagtgagaac cgccacaggc
    15421 ttccatcgcg atggttgttg ccgggcatgt cgccagaaat tcgattagct ttggtcgggt
    15481 gaatttttta cggtaaacgg ccttcccacg atgatcctga caatgaatat ggaaagagtt
    15541 cttacccaga tcgataccaa taagcgcaat gttttccatg atggttctcc gaatgaaagc
    15601 ctgtcctcag catagtactg ggaaggaggg agtgaccatc tcattaaata aagcacgcta
    15661 agccggttgc agcggtcgta gcggcctgaa cttgcccgcg ccgatcttgg cgctgctgcg
    15721 ccagaggtaa tcgccggtga ggttgatgtg ctcccaaccg agcggcgaca ggtactgcaa
    15781 cagcgcgtca tcaacggcat ggccgttgcc acgcagcgcg tgcgcagccc gttccagata
    15841 gaccgtgttc cacaacacga cggcagccgt taccagattg aggccgctag cccggtagcg
    15901 ctgctgctcg aaactgcggt cgcggatttc acccaggcgg ttgaaaaaca ctgccctggc
    15961 cagcgcattg cgcgcctcgc ccttgttcag gccggcatgc acgcggcggc gcagttccac
    16021 gctttgcagc cagtccagga tgaacagcgt gcgctcgatg cggcccagct cgcggagcgc
    16081 cacggccagg ccgttctggc gtgggtagct gccgagcttt cggagcatca gggaggccgt
    16141 caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat gggcgcggac
    16201 gtgcttgatg ttgagcgtgc cgccgatcat gggtttcagc gcgtcatagg cggcgtcgcc
    16261 cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga agcggaagcc
    16321 caggaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg tgtaatgctc
    16381 ctcgatccgc aagtcggact cgtggtacag caggccgtcg agcacgtagg tcgaatcgcg
    16441 cacgccgacg ttcacgacct tggtgtgaaa tggcgcgtac tggtcagaaa tgtgggtgta
    16501 gaacgtccgc cctgggctgc tcccgtattt cgggttgatg tggccggtgc tctcggcctt
    16561 gctgccggtg cggaagttct ggccgtccga cgatgaggtg gtgccgtcgc cccagtgctc
    16621 ggcgaaggga tggcggaact gtgcgttgac cagatcggcc agcgccgccc cgtaggtttc
    16681 gtcgcggatg tgccaggctt gcagccaagc cagcttggcg taggtcgtgc cggggcaaga
    16741 ctccgccatc ttggtcaggc ccaggttgat cgcgtcggcg aggatcgtgg tcagcaacag
    16801 gttcttgtct ttggccgggt cgcccgattt cagatgcgcg aaatgccgag tgaagcccgt
    16861 ccattcgtcc acctccagca gcagttcggt gatcttgacg tgcggcagga tcattgccgt
    16921 ctggtcgatc agcgcttggg cggtgtcggg taccgccgcg tcgagcggcg tgatcttcaa
    16981 gcctgactcg gtgatgatgg cgtccggcag ctcgttggcc gtcgccatac ggttgacggt
    17041 ggcaagctgt gtttccagca gcgtcagccg gtcgttcagg taccggttgc agtcggtggc
    17101 cacggccagc ggcaattcgc tggcctgctt gaggctggcg aatttcgcgg gcggcaccag
    17161 gtagtcctcg aagtccttga actggcgcga cccctgcacc cagatgtcgc cggaacgcaa
    17221 cgcgttcttc atctccgaca gcgcgcacag ttcgtagtag cgccggtcga tgccggtgtc
    17281 ggtcatgacc agcttctgcc agcgcggctt gatgaactcg gtcggcgcgt cggcgggcac
    17341 cttgcgggcg ttgtcgctgt tcatgccgcg cagcacctcg atggcgtcga gcacgtcctt
    17401 cgcggcggga gcggcccgca gctggcactg ttgcaaagtt agcgatgagg cagccttttg
    17461 tcttattcaa aggccttaca tttcaaaaac tctgcttacc aggcg
//
LOCUS       DAJVRL010000081        17386 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVRL010000081 DAJVRL010000000
VERSION     DAJVRL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.081"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(43..774)
                     /gene="rlmB"
                     /locus_tag="OQN02_002825"
     CDS             complement(43..774)
                     /gene="rlmB"
                     /locus_tag="OQN02_002825"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1643546.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(954..3395)
                     /gene="rnr"
                     /locus_tag="OQN02_002826"
     CDS             complement(954..3395)
                     /gene="rnr"
                     /locus_tag="OQN02_002826"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1643547.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="OQN02_002827"
     CDS             complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="OQN02_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1643548.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4064..5362)
                     /gene="purA"
                     /locus_tag="OQN02_002828"
     CDS             complement(4064..5362)
                     /gene="purA"
                     /locus_tag="OQN02_002828"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1643549.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5466..5663)
                     /locus_tag="OQN02_002829"
     CDS             complement(5466..5663)
                     /locus_tag="OQN02_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1643550.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="OQN02_002830"
     CDS             complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="OQN02_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1643551.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="OQN02_002831"
     CDS             complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="OQN02_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1643552.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="OQN02_002832"
     CDS             complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="OQN02_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1643553.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRACTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="OQN02_002833"
     CDS             complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="OQN02_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1643554.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="OQN02_002834"
     CDS             complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="OQN02_002834"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1643555.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="OQN02_002835"
     CDS             complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="OQN02_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1643556.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="OQN02_002836"
     CDS             complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="OQN02_002836"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1643557.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="OQN02_002837"
     CDS             complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="OQN02_002837"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1643558.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="OQN02_002838"
     CDS             complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="OQN02_002838"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1643559.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15982..17121
                     /gene="queG"
                     /locus_tag="OQN02_002839"
     CDS             15982..17121
                     /gene="queG"
                     /locus_tag="OQN02_002839"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1643560.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17104..17157)
                     /locus_tag="OQN02_002840"
     CDS             complement(17104..17157)
                     /locus_tag="OQN02_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643561.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3943 a         4959 c         4367 g         4117 t
ORIGIN      
        1 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
       61 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
      121 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
      181 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
      241 cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
      301 cgcaaggtta gtcacccgaa tcagcggaac gctttctgcc gcgccgcagg ctactttttt
      361 cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcat gaacgcctgc
      421 ggcgtccgca ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc cgtcgaggat
      481 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
      541 acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
      601 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
      661 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
      721 ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt
      781 actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg atctctgcaa
      841 tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga
      901 acaaagcgca ttttgtcagc aatctaaccc tcttttttta aagagggtat tgatcactct
      961 gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt ctgcgttttc
     1021 gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt cgtttttttc
     1081 tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg acgacgcttg
     1141 ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg
     1201 cgcggtgcgc gttcactgga gatcagacta aagtcgattt tgcgctcgtc catattaacc
     1261 gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct ggattccccc
     1321 atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg
     1381 accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg
     1441 gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag
     1501 tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc
     1561 agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt gttgccctga
     1621 tgcccctgct ctttcgccag cagatattta atggcgcggt gcagcgtcag gtcaggataa
     1681 cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa gtgaccacgg
     1741 ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg cagcatttct
     1801 gcatcaggac ggtcggcaac cgactccagc agttccgcgt agtcacgcgg ttccggcttg
     1861 ttaccacccg gcagctccag ccccagctcc gccagcactg aacggaaaga ggtaatcgct
     1921 tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt ctcaacgaaa
     1981 cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg cgcgtcgtta
     2041 cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct
     2101 tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac tttatagagg
     2161 ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag atcctgatcg
     2221 ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct catcaccgct
     2281 tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata
     2341 cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac ttccggcagc
     2401 atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc ttctctgtcc
     2461 agcggcgttg gcggacgcac atagtagctg acgtcggcaa tcgcgaccca taaacgccag
     2521 ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc gtcttcgcca
     2581 tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc ttcttccggc
     2641 acttcttctt tcagccccgc aacctgttgc tcaacagcct gcggccagat atacggaatt
     2701 tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattgtc gcccagcact
     2761 tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag ttcgactacg
     2821 accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc
     2881 agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtaaagta gcgaccaaca
     2941 atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg
     3001 tcagcaccca gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt catctgctcg
     3061 ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta gccatcacgg
     3121 tgaccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacga
     3181 cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg caggccttca
     3241 agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt
     3301 tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt
     3361 tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg
     3421 atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca
     3481 aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc ttagaaagcg
     3541 cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg
     3601 ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca
     3661 ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat
     3721 tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac
     3781 tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag
     3841 tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat acatttatat
     3901 tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa
     3961 gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac
     4021 actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg tcgaacgggt
     4081 cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg atcggcacac
     4141 cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcagg ccgctacgat
     4201 ctttcacgcc gaaagtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctacac
     4261 ctttccagtc gtcagctgcc agcggagtgg tagtcacttc gcgaccatcc ggcatacggt
     4321 aagccacgca gagtttaacc tctttcaggc catccagaac gtccagttta gtcaggcaga
     4381 agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc agccagccgg
     4441 tacgacgacg acgacccgta gttgcaccga attcgttacc ctgcttgcag aggaactcgc
     4501 cagtttcatc aaacagttcg gccgggaaag gacctgcacc tacacgagtt gagtaagctt
     4561 tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg gtcgccacgc
     4621 caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg atatccagca
     4681 gcgtaccctg cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc tggtcgagca
     4741 ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc gtatcatcca
     4801 gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat
     4861 attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac
     4921 gcagaccgcg acgtgccact ttatcttcat aagcaggccc gataccacga ccggtggtgc
     4981 cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagagcaacg tgataatcaa
     5041 ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg ccacggtctt
     5101 ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg ttaccgatga
     5161 tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac
     5221 cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag
     5281 cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca
     5341 gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc
     5401 atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt
     5461 tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt
     5521 ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc
     5581 ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc
     5641 gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc
     5701 ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa
     5761 gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat cacgtcctga
     5821 ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga
     5881 tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc accacgcata
     5941 atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg
     6001 cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc
     6061 tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgcttg
     6121 atacgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga
     6181 actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgct
     6241 gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact
     6301 tcgagggtca gacgaccacg ggaatcggtg acgatatctt tcacgtccag gcgaccaatt
     6361 tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg
     6421 ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt gatgtaagag
     6481 tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc
     6541 tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagacca
     6601 ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta
     6661 ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg
     6721 atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac
     6781 gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc
     6841 ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt
     6901 tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac ggcagaacca
     6961 tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac actttttcca
     7021 tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa
     7081 gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac
     7141 gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggtat
     7201 acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa
     7261 acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga
     7321 tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca
     7381 cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact ccacgcaggg
     7441 cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt
     7501 ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg
     7561 tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc ggtttgactt
     7621 cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg aatttaccaa
     7681 agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga
     7741 taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc
     7801 cttgcgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga ccaccgagct
     7861 ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt
     7921 tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt
     7981 gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga
     8041 tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag
     8101 atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc
     8161 atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga
     8221 agctgataaa aacgactcct cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc
     8281 gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg
     8341 gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc aatacgcggt
     8401 tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagcg
     8461 tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc
     8521 gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt ggctttaaat
     8581 gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc aagtacggtt
     8641 tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg
     8701 tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg
     8761 gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg ccgcccctgc
     8821 tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg attacgcaac
     8881 aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat cccgcctttc
     8941 tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg cagctgcgcc
     9001 agctcaacct gcaacttacc ctcatgggta cacgcacgtt gggcgaaaat atctaaaata
     9061 aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc
     9121 gggctcaggg catggtcaaa aagaacgacc gaagcaccca tcgctttgac agcttccgca
     9181 atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg
     9241 gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg
     9301 tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca
     9361 tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc
     9421 tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc
     9481 gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc gccaccggcg
     9541 ttgttactgt gatgagaaac cgggcgagat gggacaacag tagaaatcgc gtgcttgtaa
     9601 accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc
     9661 ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt
     9721 gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg
     9781 cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt
     9841 acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg
     9901 tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt
     9961 cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc
    10021 ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca agtccgtatg
    10081 caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg
    10141 atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa
    10201 ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt
    10261 acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat
    10321 ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact cccaaccttg
    10381 ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg gtagcggcga
    10441 caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg
    10501 ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga
    10561 gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc
    10621 agcgttcggc ttcgccgtcc cgataaccat ccctttgtaa ataagggcag aatcaacgct
    10681 tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc
    10741 cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt
    10801 tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt
    10861 tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc tgtgccattg
    10921 accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt
    10981 cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt
    11041 gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct gactggaaat
    11101 caatacccaa ttccgctagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag
    11161 aaacttttag ccgcaacgga atcagcagcg gctgggcgca aacgggcgct tcacccggcg
    11221 tcaattgtgc ctgacgcagc caacgttctg ccactggcaa ggataaaagt gaaatgttgc
    11281 catcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct
    11341 gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttggtttt tgcatcggcg
    11401 cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag cctggctgcg
    11461 cattcggcca gggggcagcc ggacgactgc ctgaggcggg cgcaggagag tagcgcggag
    11521 ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc acgcggtttt
    11581 ccgggatggc acgcggtgcg ggttgtggtt catcgtccag cggtagcggc gtttccagtt
    11641 gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga cgcgactgat
    11701 ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc cacctgatgc gggtcgatct
    11761 ccagatacaa cacaaatgcc ggttgctgat cggcccccag tttgtcttcg caggcctggc
    11821 ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc attcacgtag caatactgaa
    11881 tttccgccag tgcgggcgta gtgtgatttg gatcggccac ccagccgcgc agggtgagat
    11941 cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc
    12001 ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc acaattttac
    12061 cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg cggatgattt
    12121 catcaatatg gctaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt
    12181 tgtagaacag atccagcacc tccagcgtcg ttcccaccgg atgcgctgcc ggtttaaccg
    12241 tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc
    12301 gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc
    12361 ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgag
    12421 ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga atgcggataa
    12481 gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt
    12541 tttccactag ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct
    12601 ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg
    12661 tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt
    12721 gcatcggatg cgcaaggaag taattgcgca ggcctttgta aatggcttct gccagctgtt
    12781 gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata aaaccggttt
    12841 cgaccagtac tgatgggata tccggcgagc gcagaacgcc aaggctggcg tgttctggtc
    12901 gacgtttatg tatttcgcca atgcgttgca actgactgat catactggtc gctacatcat
    12961 accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt aaataggggt
    13021 cagactgact gttcgccagc acatcacccg ccccacccag cagctccgac agtttctcgt
    13081 actgctccag ccagctggcc atttcactgt tggcgcgacg gttagagagc acccatacgg
    13141 aagcgccagt cgcactacgg ttcggtgcgg catcagcgtg aatcgacacg aggaaattgg
    13201 cgttttgctt atgtgccaca tcactgcgcc ccatcaccga gataaagtaa tccccgtcac
    13261 gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat ttacgcgcga
    13321 tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca gggtcctgac
    13381 cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg
    13441 ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt
    13501 taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc
    13561 gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta atcgtaaaga
    13621 cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg ttttcggtaa
    13681 gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga atcgccttca
    13741 ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt tgtttgatat
    13801 cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg ccaataaaac
    13861 tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag agcgtcgcgg
    13921 cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg atgcgataca
    13981 tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag
    14041 aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg tgtatttcga
    14101 catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg cagatggcat
    14161 cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg gcaaggcggt
    14221 acaaatcaaa gtgatagacc attaagttgt cgagcgtata gggttcgacc agcgtataag
    14281 tggggctttt gacattaccc tgatgaccca gagcctgtaa aaagccccgg ctaaaggtgg
    14341 ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg caggctttcg
    14401 ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt actcgattca
    14461 tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga aaagagatcg
    14521 gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc
    14581 gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag caatgcgcca
    14641 ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt tccggcatca
    14701 ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt cagcaccgct
    14761 acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact ttcaatttca
    14821 gcgacggaac agcctaacaa ccgtgcggcc tcgctaggat gcggcgtgat cacgcgattg
    14881 tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca caacatcggt
    14941 ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg gcccagaccg
    15001 ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc catagtcagt
    15061 tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg caatgttctc actgcgggtc
    15121 agtactcgga ctaaaccagc accagcacgt agcgccgctt cccccgtcat acgaatagcc
    15181 cccgccgtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc tttatgcgaa
    15241 gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc
    15301 gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag ttgtcctgta
    15361 acatcccgcg cttttccagt gagcaagcct agtttcagcg caataaaagt gatggtgtga
    15421 tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc ggaagggata
    15481 tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg gctaatggat
    15541 tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag atctaccgat
    15601 tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca tgcttcgcgt
    15661 gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggccaacaa cgtgacctca
    15721 atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt attaccatga
    15781 ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac ctggaatgcg
    15841 gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc tgccgcctcg
    15901 cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact tacggggttt
    15961 ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt agcgcaaaaa
    16021 attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga taccgatctc
    16081 agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg cgaaatggac
    16141 tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattacc cggtacgctg
    16201 cgtgtaatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc cagcacgctg
    16261 aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta tcacaaactt
    16321 ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt ttcgctgaat
    16381 tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga aaaagctggg
    16441 ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc gttcttcttt
    16501 ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga ggaaggatgc
    16561 ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga gccatatacc
    16621 gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc gctcccggaa
    16681 gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca gcttatctgc
    16741 ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgcg taagccgcta
    16801 cacgcaccgg aactcattga gttatttgcc tggagcgaag agaagttttt aaaagtcacg
    16861 gaaggatcgg cgattcgccg tataggtcac ctgcgctggc tgcgtaatat cgccgtggca
    16921 ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg taaaggtgag
    16981 cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga gagacgaaat
    17041 gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt gattgaaaaa
    17101 gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa aacgcattgc
    17161 aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc ttgattttta
    17221 acttatttat caataggtta aaaaatacca ttcaccgtgc gaagtttctt tgaaattaag
    17281 attacagcct gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacaaaaac
    17341 cagcaataac gcatcgcgtc gctgtagata ttttattgag agaaga
//
LOCUS       DAJVRL010000082        17173 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVRL010000082 DAJVRL010000000
VERSION     DAJVRL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.082"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..185
                     /locus_tag="OQN02_002841"
     CDS             <1..185
                     /locus_tag="OQN02_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCS1643562.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="OQN02_002842"
     CDS             complement(210..401)
                     /locus_tag="OQN02_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCS1643563.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="OQN02_002843"
     CDS             complement(506..742)
                     /locus_tag="OQN02_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCS1643564.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OQN02_002844"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="OQN02_002844"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS1643565.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OQN02_002845"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="OQN02_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OQN02_002846"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="OQN02_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS1643566.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="OQN02_002847"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="OQN02_002847"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1643567.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="OQN02_002848"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="OQN02_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1643568.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="OQN02_002849"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="OQN02_002849"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1643569.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="OQN02_002850"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="OQN02_002850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1643570.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="OQN02_002851"
     CDS             complement(10860..12233)
                     /locus_tag="OQN02_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1643571.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="OQN02_002852"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="OQN02_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1643572.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="OQN02_002853"
     CDS             complement(13344..13583)
                     /locus_tag="OQN02_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643573.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="OQN02_002854"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="OQN02_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1643574.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="OQN02_002855"
     CDS             13960..14247
                     /locus_tag="OQN02_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1643575.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14826..14969
                     /locus_tag="OQN02_002856"
     CDS             14826..14969
                     /locus_tag="OQN02_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1643576.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14982..15191
                     /gene="cspE"
                     /locus_tag="OQN02_002857"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14982..15191
                     /gene="cspE"
                     /locus_tag="OQN02_002857"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1643577.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15357..16166
                     /gene="rlmA"
                     /locus_tag="OQN02_002858"
     CDS             15357..16166
                     /gene="rlmA"
                     /locus_tag="OQN02_002858"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1643578.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="OQN02_002859"
     CDS             complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="OQN02_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1643579.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4532 a         4155 c         4337 g         4149 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggttgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga
    14401 tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac
    14461 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
    14521 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
    14581 tctggcctgg aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc
    14641 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
    14701 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
    14761 ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc
    14821 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
    14881 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
    14941 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
    15001 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
    15061 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
    15121 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
    15181 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
    15241 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
    15301 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt
    15361 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
    15421 cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
    15481 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
    15541 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
    15601 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
    15661 catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
    15721 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
    15781 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
    15841 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
    15901 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
    15961 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
    16021 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
    16081 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
    16141 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
    16201 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
    16261 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
    16321 cggtcgtgat aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca
    16381 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt
    16441 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
    16501 gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca
    16561 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
    16621 atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg
    16681 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct
    16741 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
    16801 gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
    16861 gactgaaagt cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
    16921 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
    16981 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg attttcattt
    17041 atgtttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc
    17101 ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt
    17161 attgagttga tac
//
LOCUS       DAJVRL010000083        17051 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVRL010000083 DAJVRL010000000
VERSION     DAJVRL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.083"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..213)
                     /locus_tag="OQN02_002860"
     CDS             complement(<1..213)
                     /locus_tag="OQN02_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCS1643580.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OQN02_002861"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OQN02_002861"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1643581.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OQN02_002862"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OQN02_002862"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1643582.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OQN02_002863"
     CDS             2894..3634
                     /locus_tag="OQN02_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1643583.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OQN02_002864"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OQN02_002864"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1643584.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="OQN02_002865"
     CDS             complement(4192..4794)
                     /locus_tag="OQN02_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCS1643585.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4863..4928
                     /gene="yobI"
                     /locus_tag="OQN02_002866"
     CDS             4863..4928
                     /gene="yobI"
                     /locus_tag="OQN02_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1643586.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5067..5678
                     /gene="ruvA"
                     /locus_tag="OQN02_002867"
     CDS             5067..5678
                     /gene="ruvA"
                     /locus_tag="OQN02_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1643587.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5687..6697
                     /gene="ruvB"
                     /locus_tag="OQN02_002868"
     CDS             5687..6697
                     /gene="ruvB"
                     /locus_tag="OQN02_002868"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1643588.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="OQN02_002869"
     CDS             complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="OQN02_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1643589.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="OQN02_002870"
     CDS             complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="OQN02_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1643590.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8460..9392
                     /gene="znuA"
                     /locus_tag="OQN02_002871"
     CDS             8460..9392
                     /gene="znuA"
                     /locus_tag="OQN02_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1643591.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9408..10730
                     /gene="mepM"
                     /locus_tag="OQN02_002872"
     CDS             9408..10730
                     /gene="mepM"
                     /locus_tag="OQN02_002872"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1643592.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10850..11821
                     /gene="lpxM"
                     /locus_tag="OQN02_002873"
                     /gene_synonym="msbB"
     CDS             10850..11821
                     /gene="lpxM"
                     /locus_tag="OQN02_002873"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1643593.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="OQN02_002874"
     CDS             complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="OQN02_002874"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1643594.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13522..14391)
                     /gene="hexR"
                     /locus_tag="OQN02_002875"
                     /gene_synonym="yebK"
     CDS             complement(13522..14391)
                     /gene="hexR"
                     /locus_tag="OQN02_002875"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1643595.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14728..16203
                     /gene="zwf"
                     /locus_tag="OQN02_002876"
     CDS             14728..16203
                     /gene="zwf"
                     /locus_tag="OQN02_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1643596.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16438..>17051
                     /locus_tag="OQN02_002877"
     CDS             16438..>17051
                     /locus_tag="OQN02_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1643597.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDR"
BASE COUNT         4352 a         4205 c         4433 g         4061 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagatacg aatatacgta atagcataaa aaattatttt tcaggagtcg
     4861 caatgtatat ttttattacg catttcttca ctgaatatgt aatattcaaa tatttgcttc
     4921 caatataacc tgttgaataa attatactgt gccatttttc agatcatcgg gacacctcgc
     4981 aagttttctt catccttcgc tggatatcta tccagcattt ttttatcata cagcattatc
     5041 tttgattcat tacgcaggag cgtcatgtga taggcagact cagaggcatc atcattgaaa
     5101 aacaaccccc gctggtgtta attgaagtgg gcggcgtagg ctatgaagtg catatgccga
     5161 tgacctgttt ttatgaactc cctgaagcgg gtcaggaagc gatcgttttc acccactttg
     5221 tggtgcgtga agacgcgcaa ctgctgtacg gttttaacaa taaacaagaa cgcacgttgt
     5281 tcaaagagtt gatcaaaacc aacggcgtcg gcccgaagtt ggccctggcg atcctctccg
     5341 gaatgtcagc gcagcagttc gttaatgccg ttgagcgtga agaagtgggg gcactggtga
     5401 aactgccggg tattggcaaa aaaaccgccg aacgcttgat tgttgaaatg aaagaccgat
     5461 ttaaaggttt gcatggcgat ctctttacgc cagccgccga cctggtactc acgtctcctg
     5521 ctagcccggc gaccgacgat gctgaacaag aagcagttgc cgcgctggtg gcgctaggct
     5581 ataaaccaca agaagcaagc cgcatggtga gcaaaatcgc tcgccctgac accagcagtg
     5641 aaacattaat tcgcgaagcc ctacgcgccg cgttatgagg taaaggatga ttgaagcaga
     5701 ccgtctgatt tctgccggta ccactttgcc ggaagatgta gcagatcgcg ccattcgccc
     5761 taaattactg gaagagtatg ttggtcagcc gcaggttcgt tcacagatgg agattttcat
     5821 caaagcagcg aaactgcgcg gcgatgccct cgatcatttg ttgatttttg gtcctccggg
     5881 gttgggtaaa actacgcttg ccaacattgt cgccaatgaa atgggcgtta atttacgcac
     5941 gacttctggt ccggtgctgg aaaaggcggg cgatttggct gcgatgctca ctaaccttga
     6001 accgcatgac gtgctgttta ttgatgagat ccaccgttta tcgccagttg ttgaagaagt
     6061 gctgtacccg gcaatggaag actaccaact ggatatcatg attggtgaag gtccggcggc
     6121 acgctccatt aaaattgatt tgccgccgtt taccctgatt ggcgcaacca cgcgcgcagg
     6181 ttcgctgaca tcaccgttgc gcgaccgttt tggtattgtg caacgtctgg agttttatca
     6241 ggtgccggat ctgcaatata tcgtcagtcg cagtgcacgc tttatggggc ttgagatgag
     6301 tgatgacggt gcgcaggaag tagctcgtcg cgctcgcggt acgccacgta ttgccaaccg
     6361 tctgctgcgt cgagtgcgtg atttcgccga agtgaagcac gatggcacca tctcggcaga
     6421 tatcgctgct caggcgctgg atatgttgaa tgtcgatgct gaaggtttcg attatatgga
     6481 ccgcaaattg ttgctggcgg taatcgataa gttctttggt gggccggtag gtctggataa
     6541 cctggcggca gccattggcg aagaacgtga aaccattgaa gatgtactgg aaccttattt
     6601 gattcagcaa ggctttttgc agcgtacacc gcgcgggcgt atggcgacga cgcgggcgtg
     6661 gaatcacttt ggcataacgc cgccagaaat gccgtaagtc ggattggtcg ctcgcgccgc
     6721 atccgacact ggcagtgtgt ctgatgcgac actgttcgcg tcttatcagg ccagccagag
     6781 atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat
     6841 agattagctg gcctgctttt tcatcatact gataataaat aacagtgccg cacatagcac
     6901 caccgacgga cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgc cagttactgc
     6961 caccatcccc accaaaacag cgacaccagc catctgttcc ggcgtacggg caaagcgacg
     7021 cgcagtagct gcaggaataa tcagcaacga agtaataatc aacgcaccga caaatttcat
     7081 cgctacacca atcgtcaatg ccgtcaccag catcaacaac aatttcactt gctgtaattt
     7141 cacaccatca acaaacgcca gatccgggct aatcgtcatc gacagcaaat tgcgccattg
     7201 ccagaacaaa atagccacca cgatgaccac gccaatcgca atagagatga gatcttctgg
     7261 cgtcactgcc agtaaatcac cgaacaggta agccatcaaa tcaacacgaa tgttagacat
     7321 cagactaacg accactaggc ccagcgacag ggcactgtgc gccataatcc ctaataacgt
     7381 gtcgatcgcc agctgtggac gcttctccag ccataccaga ccgcccgcca gcagcagcgt
     7441 aacggcaatc accgcataga atggattcac gtccagcaac aaaccaaacg cgacaccaag
     7501 taatgaggca tgagccagcg tatcaccgaa ataagacata cgacgccaga ctacaaacga
     7561 acccagcgga cccgcggcac aggcgagcat gatcccggct aaccaaccgg gaaataataa
     7621 ttcaatcatg agcgatcatt tccccgacgc aaaacaattc gtccctgtaa atcgtgacga
     7681 tgattatgat gatggcgata gatacccagt tgttcagcac cacgaggacc aaacattgaa
     7741 ataaactccg gatgcaggga aacaacttcc ggtgtgccgg aacaacaaat gtggtgattc
     7801 aggcacagca cttcatcggt ttttgccatc accagatgca gatcgtgaga aaccattaaa
     7861 acgccacaat ccagttcacg acgcaactgg tcaataaggt catataacgc cacttggcca
     7921 tttacatcca cgccttgagt gggttcatcc agcactaata attgcgggca atttaacaat
     7981 gctcgcgcta atagtacacg ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta
     8041 atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca
     8101 gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag atacagcttc
     8161 tgcggtacat agccgatgcg cagttttccg ttgcgcttga taaccccttc atcgggtgtt
     8221 accagcccga gcactacccg taccagtgtc gacttacctg cgccatttgg cccaagtaaa
     8281 gtcaaaattt ttccaggttt aagttccagc gacacatcag agaggacgcg gcgttggcca
     8341 aaagaaaccg agacattttc cagggaaacc agacttgtca tgttaatttt agtcttgcag
     8401 tagtcatgaa atgttataat atcacacttc tcatattcat tacgatgatt ggtcgcatta
     8461 tgttacataa aaaaacgctt cttttcgcag cattatccgc cgctctctgg ggaggtgcaa
     8521 cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg
     8581 ccattgctga tggtgtaaca gaaacggagg ttttactgcc tgacggcgct tcagaacatg
     8641 attattcact gcgtccatcg gatgtaaaac gcttacagaa cgcggactta gtcgtttggg
     8701 ttggcccgga gatggaagcg tttatgcaaa aaccggtaag caaattacca gaagcgaagc
     8761 aggtaacgat tgcgcagctt gaagatgtga aaccgctact gatgaaaagt attcacggcg
     8821 atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca
     8881 acatgcatct ttggctttcc ccagagatag cgcgggctac agcggttgca atccatggaa
     8941 aattagtgga acttatgccg caaagtcgag ccaaacttga cgccaacctg aaggattttg
     9001 aggcacaatt ggcctcaacc gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga
     9061 aaggttattt cgtttttcac gatgcttacg gctatttcga aaaacagttc ggactgacac
     9121 cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa
     9181 taagaacaca gttggttgag caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc
     9241 cagcggtcgt tgagagcgtc gcacgaggga catccgttcg tatgggaacg ttggatcccc
     9301 tggggacgaa tatcaaactg ggtaaaacaa gctattcaga attcctgagt caattagcca
     9361 accagtatgc gagctgcctg aaaggagatt aatgaggaag tgattacgtg caacagatag
     9421 cccgctctgt cgccttggcg tttaataatt taccgcgacc acaccgcgtt atgttggggt
     9481 cgctcaccgt tcttactctg gccgtcgctg tctggcggcc ctatgtttat caccgtgatg
     9541 ccacgccaat tgtcaaaacc attgagctgg aacagaacga aattcgttcg ctcttacctg
     9601 aagccagtga gccgattgat caagctgcac aagaagatga agccattccc caggacgaac
     9661 tggatgacaa aatcgccggt gaagcgggcg tgcatgaata tgttgtttcc actggcgata
     9721 cgctaagcag cattctcaat cagtatggta ttgatatggg tgatatcacc caacttgctg
     9781 ctgccgacaa agaattgcgt aacctgaaaa tcggtcaaca actctcctgg acattaaccg
     9841 cggacggcga actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg
     9901 accgtactgc cgctaacggt tttaaaatga ccagcgaaat gcagcaagga gagtgggtta
     9961 acaatctgct gaaaggtacc gtcggaggga gctttgttgc cagcgccaga aacgccggtt
    10021 taaccagcgc cgaagtgagc gcagtgatta aagccatgca gtggcaaatg gatttccgca
    10081 aactgaaaaa aggcgatgaa tttgcggtat taatgtctcg agaaatgctt gatggtaaac
    10141 gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat tattacgcaa
    10201 tccgcgctga ggatggcaaa ttctacgatc gtaacggtac tggtctggcg aaaggattct
    10261 tgcgattccc gacggcgaaa cagttccgta tctcgtctaa ctttaacccg cgtcgaacta
    10321 atccggtgac aggtcgcgtt gcaccacaca gaggtgttga tttcgccatg ccacaaggta
    10381 cgccagtgct ttcagtgggt gacggtgaag tggtggttgc caaacgcagt ggcgcagcag
    10441 gttattatgt ggctattcgt catggtcgca gctacaccac gcgttatatg cacttgcgca
    10501 agattctggt gaaaccggga cagaaggtga aacgtggcga ccgtatcgcg ctttccggta
    10561 ataccggacg ttcaactggg ccgcatctgc actatgaagt atggataaac cagcaggccg
    10621 taaacccgct gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg
    10681 aattcctggc gcaggccaaa gagattgtgc cgcagctacg gtttgattaa ttaacatcca
    10741 ttcgcagccg gtacgcagtc agtaccggct ttttttattt ggtgcggggc aagttgtgcc
    10801 gctacactat caccagattg atttttgcct tatccgaaac tggaaaagca tggaaacgaa
    10861 aaaaaataat agcgaataca ttcctgagtt tgataaatcc tttcgccacc cgcgctactg
    10921 gggagcatgg ctgggtgtag cagcgatggc gggtatcgct ttaacgccgc caaagttccg
    10981 tgatcccatt ctggcacggc tgggacgttt tgccggacga ctgggaaaaa gctcacgccg
    11041 tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt agtgaagctg aacgcgaagc
    11101 gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc
    11161 aatacgcggg ccggagaaaa ttcagccgcg cgttggctgg caagggctgg agatcatcga
    11221 agagatgcgg cgtaataacg agaaagttat ctttctggtg ccgcacggtt gggccgtcga
    11281 tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt tccataatca
    11341 gggcaacccg gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct
    11401 gcatgcgaga aatgacggta ttaaaccatt catccagtcg gtacgtcagg ggtactgggg
    11461 atattattta cccgatcagg atcatggccc agagcacagc gaatttgtgg atttctttgc
    11521 cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg aaagtatgcc gtgcgcgcgt
    11581 tgtaccgctg tttccgattt atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc
    11641 accgatggat gatctgttag aggcggatga tcatacgatt gagcggcgga tgaatgaaga
    11701 agtcgagatt tttgttggtc cgcgaccaga acaatacacc tggatactaa aattgctgaa
    11761 aactcgcaaa ccgggcgaaa tccagccgta taagcgcaaa gatctttatc ccatcaaata
    11821 aaaaagcctc tcgcgaggag aggccttcgc ctgatgataa gttcaagttt gcttcagaat
    11881 attcgaaatc tgttgaacta tcattgaact ataggccgga tgtggcgttt tcgccgcatc
    11941 cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc cacggtactc
    12001 atcacgtcgc cttgggtgac aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc
    12061 agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc aaagtgcacc
    12121 ggcgtaacgc cacgatagag agcagtcagg ttcagcgtac gttcatggcg cgacatggcg
    12181 aaaattggta gaccagagct gatacgggag gtcatcagcg cggtacgacc cgattcggtc
    12241 atggtaatga tcgccgtaac gcccttcagg tggttagctg cgtacattgc cgacatggca
    12301 atagcttctt ccacattgtc gaactgaacg tccagacggt gtttagaaac gttgatgctc
    12361 gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc tgacggatac
    12421 tgcccagcgg cagtttctgc agacagcatc acagcgtcag taccatccag aacggcgttt
    12481 gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga ctccatcatc
    12541 tgggtcgccg tgattaccgc tcggtttagc tgacgcgcac gacggatcaa cgctttctga
    12601 atgccgacca gttccgggtc gccaatttcc acaccgaggt cgccacgtgc aaccattacc
    12661 acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt
    12721 tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccaggcg acgggcatag
    12781 ttcagatctt cgccacagcg tgggaaggag acagccaggt aatctacgcc aatcaacgcc
    12841 gcagtcttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa accgccgcca
    12901 agtttgttga tacctttatt gttggagagg ggaccaccga cggtgacttc ggtgaacact
    12961 ttcatgccct gaacttccag tacttttaac tggacgcgac catcgtccag cagcaggatg
    13021 tcaccaggca cgacgtcagc aggcaggcct ttgtagtcga taccgacttt ttctttgtcg
    13081 ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt gaggaaaact
    13141 ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc cagaatagcc
    13201 acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat tttgtgatct
    13261 tcaggcgagc cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc gataactttt
    13321 tcaagattat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt tgttctgcga
    13381 agccttttgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct tgaacatgaa
    13441 atccgaatga aataacgccg cgatggcgat aaattaatct cattcggatg tcgtataaga
    13501 ttaggacagt gacagtcgct tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa
    13561 acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg
    13621 tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac
    13681 catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc
    13741 ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atggcgtcgt tttcgcgtgc
    13801 cagctgcgcc agctcgacca gattttttgt tcttcctgtg tgagaaatca gcaccaccac
    13861 gtctccgtcg ctacaattca tacaactcat gcgttgcagc acgatatcat cggagtacac
    13921 caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg cggcagaaga
    13981 gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat
    14041 ggcagattta tccagtgaat gacggacatg atcgagcgtt gccattgcgg actcaaatat
    14101 tttccccgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa catagggagt
    14161 gccattcgcc agactttgtg ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat
    14221 gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagcg ccagagcagc
    14281 aatactcgaa tggatcgcgt tatcgggcga agccagaatg acctcggcaa ctttgcgctc
    14341 tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca tgatgtaaag
    14401 agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt tttgtcagat
    14461 attacgcctg tgtgccgtgt taatgacaaa agcagataaa aaagttgtta tttttttcat
    14521 aacatgatca gtgtcagatt tttaccgaat ggaaaacgat gattttttta tcagttttgc
    14581 cgcactttgc gcgcttttcc cgtaatcgca cgggtggata agcgtttaca gttttcgcaa
    14641 gctcgtaaaa gcagtacagt gcaccgtaag aaaattacaa gtataccctg gcttaagtac
    14701 cgggttagtt aacttaagga gaatgacatg gcggtaacgc aaacagccca ggcctgtgac
    14761 ctggtcattt tcggcgcgaa aggcgacctt gcgcgtcgta aattgctgcc ttccctgtat
    14821 caactggaaa aagtcggtca gctcaacccg gacacccgga ttatcggcgt agggcgtgct
    14881 gactgggata aagcggcata taccaaagtt gtccgcgagg cgctcgaaac tttcatgaaa
    14941 gaaaccattg atgaaggttt atgggacacc ctgagtgcac gtctggattt ttgtaatctc
    15001 gatgtcaatg acactgctgc attcaaccgt ctcggcgcga tgctggatca aaaaaatcgt
    15061 atcactatta actactttgc catgccgccc agcacttttg gcgcaatttg caaagggctt
    15121 ggcgaggcaa aactgaatgc taaaccggca cgcgtagtca tggagaaacc gctggggacg
    15181 tcgctggcga cctcgcagga aatcaatgat caggttggcg aatacttcga ggagtgccag
    15241 gtttaccgta tcgaccacta tcttggtaaa gaaacggtgc tgaacctgtt ggcgctgcgt
    15301 tttgctaact ccctgtttgt gaataactgg gacaatcgca ccattgatca tgttgagatt
    15361 accgtggcag aagaagtggg gatcgaaggg cgctggggct attttgataa agccggtcag
    15421 atgcgtgaca tgattcagaa ccacctgcta caaattctct gcatgattgc gatgtctccg
    15481 ccatctgacc tgagcgcaga cagcatccgc gatgaaaaag tgaaagtact gaagtccctg
    15541 cgccgcatcg accgctccaa cgtacgcgaa aaaaccgtac gcgggcaata tactgcgggc
    15601 ttcgcccagg gcaaaaaagt gccgggatat ctggaagaag agggcgcgaa caaaagcagc
    15661 aatacagaaa ccttcgtggc gatccgcgtc gacattgata actggcgctg ggccggtgtg
    15721 ccattctacc tgcgtactgg taaacgtctg ccgaccaaat gttctgaagt cgtggtctat
    15781 ttcaaaacac ctgaactgaa tctgtttaaa gagtcgtggc aggatctgcc gcagaataaa
    15841 ctgactatcc gtctgcaacc tgatgaaggc gtggatatcc aggtactgaa taaagttcct
    15901 ggccttgacc acaaacataa cctgcaaatc accaagctgg atctgagcta ttcagaaacc
    15961 tttaatcaga cgcatctggc ggatgcctat gaacgtttgc tgctggaaac catgcgtggt
    16021 attcaggcac tgtttgtacg tcgcgacgaa gtggaagaag cctggaaatg ggtagactcc
    16081 attactgagg cgtgggcgat ggacaatgat gcgccgaaac cgtatcaggc cggaacctgg
    16141 ggacccgttg cctcggtggc gatgattacc cgtgatggtc gttcctggaa tgagtttgag
    16201 taatatcagc gcttatcctt tatggttatt ttaccggtaa catgatcttg cacagattgt
    16261 agaacaattt ttacactttc aggcctcgtg cggattcacc cacgaggctt tttttattac
    16321 actgactgaa acgtttttgc cctatgagct ccggttacag gcgtttcact cataaatcct
    16381 ctgaatgaaa cgcgttgtga atcatcctgc tctgacaact caatttcagg agcctttatg
    16441 aatccacaat tgttacgcgt aacaaatcga atcattgaac gttcgcgcga gactcgctct
    16501 gcttatctcg cccggataga acaagcgaaa acgtcgaccg ttcatcgttc gcagttggca
    16561 tgcggtaacc tggcacacgg tttcgctgcc tgccagccag aagacaaagc ctctttgaaa
    16621 agcatgttgc gtaacaatat cgccatcatc acctcctata acgacatgct ctccgcgcac
    16681 cagccttatg aacactatcc agaaatcatt cgtaaagccc tgcatgaagc gaatgcggtt
    16741 ggtcaggttg cgggcggtgt tccggcgatg tgtgatggtg tcacccaggg gcaggatgga
    16801 atggaattgt cgctgctaag ccgcgaagtg atagcgatgt ctgcggcggt ggggctgtcc
    16861 cataacatgt ttgatggtgc tctgttcctc ggtgtgtgcg acaagattgt cccgggtctg
    16921 acgatggcag ccctgtcgtt tggtcatttg cctgcggtgt ttgtgccgtc tggaccgatg
    16981 gcaagcggtt tgccaaataa agaaaaagtg cgtattcgcc agctttatgc cgaaggtaaa
    17041 gtggaccgca t
//
LOCUS       DAJVRL010000084        16986 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVRL010000084 DAJVRL010000000
VERSION     DAJVRL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.084"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            419..1237
                     /gene="ybhA"
                     /locus_tag="OQN02_002878"
     CDS             419..1237
                     /gene="ybhA"
                     /locus_tag="OQN02_002878"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCS1643598.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1238..2296)
                     /gene="modC"
                     /locus_tag="OQN02_002879"
     CDS             complement(1238..2296)
                     /gene="modC"
                     /locus_tag="OQN02_002879"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1643599.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2299..2988)
                     /gene="modB"
                     /locus_tag="OQN02_002880"
     CDS             complement(2299..2988)
                     /gene="modB"
                     /locus_tag="OQN02_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1643600.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2988..3761)
                     /gene="modA"
                     /locus_tag="OQN02_002881"
     CDS             complement(2988..3761)
                     /gene="modA"
                     /locus_tag="OQN02_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1643601.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3928..4077)
                     /gene="acrZ"
                     /locus_tag="OQN02_002882"
     CDS             complement(3928..4077)
                     /gene="acrZ"
                     /locus_tag="OQN02_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1643602.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4206..4994
                     /gene="modE"
                     /locus_tag="OQN02_002883"
     CDS             4206..4994
                     /gene="modE"
                     /locus_tag="OQN02_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1643603.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5062..6534
                     /gene="modF"
                     /locus_tag="OQN02_002884"
     CDS             5062..6534
                     /gene="modF"
                     /locus_tag="OQN02_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1643604.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6795..7811
                     /gene="galE"
                     /locus_tag="OQN02_002885"
     CDS             6795..7811
                     /gene="galE"
                     /locus_tag="OQN02_002885"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1643605.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7821..8867
                     /gene="galT"
                     /locus_tag="OQN02_002886"
     CDS             7821..8867
                     /gene="galT"
                     /locus_tag="OQN02_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1643606.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8871..10019
                     /gene="galK"
                     /locus_tag="OQN02_002887"
     CDS             8871..10019
                     /gene="galK"
                     /locus_tag="OQN02_002887"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1643607.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10013..11053
                     /gene="galM"
                     /locus_tag="OQN02_002888"
     CDS             10013..11053
                     /gene="galM"
                     /locus_tag="OQN02_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1643608.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11256..12008
                     /gene="gpmA"
                     /locus_tag="OQN02_002889"
     CDS             11256..12008
                     /gene="gpmA"
                     /locus_tag="OQN02_002889"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1643609.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12175..13227)
                     /gene="aroG"
                     /locus_tag="OQN02_002890"
     CDS             complement(12175..13227)
                     /gene="aroG"
                     /locus_tag="OQN02_002890"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1643610.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13543..13923
                     /locus_tag="OQN02_002891"
     CDS             13543..13923
                     /locus_tag="OQN02_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1643611.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14037..14978
                     /gene="zitB"
                     /locus_tag="OQN02_002892"
     CDS             14037..14978
                     /gene="zitB"
                     /locus_tag="OQN02_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1643612.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(14975..15694)
                     /gene="pnuC"
                     /locus_tag="OQN02_002893"
     CDS             complement(14975..15694)
                     /gene="pnuC"
                     /locus_tag="OQN02_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1643613.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15732..16775)
                     /gene="nadA"
                     /locus_tag="OQN02_002894"
     CDS             complement(15732..16775)
                     /gene="nadA"
                     /locus_tag="OQN02_002894"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1643614.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4297 a         4460 c         4218 g         4011 t
ORIGIN      
        1 gacacatccg gattgaaatg ggaaggtgac ccaccgccat ttcatgaaat ccgaagttta
       61 tccggtcgcc tgcactcagc agaaaaaggc agtgatttca ctcaggcgct ccttggacat
      121 cgttcatcca gcatgacgga taaataccgt gacggacgag ggagggaatg gaaagacatt
      181 tagaattaat ttcgaatgat ttcgaaatgc cacttgtaat taattgatta taaagaatta
      241 attcctaaag agcatttgct tcatgaatgc tcctttgcat ttagcaactg actgtaacag
      301 ctaaaattag tcgcttttgg cggtgaatgc tgaattaaaa cgcagaattt tgtccgatag
      361 tggtatcggt gtaccattcg cgcagacgaa aattcttaac cttaatctgg aatacgccat
      421 gaccacacgc gtgattgctc tcgacttaga cggcacctta ttgaccccga aaaagacctt
      481 gcttccatca tcgatagaag ccctggcccg cgctcgcgaa gcaggctatc aattaatcat
      541 cgtcacaggt cgccatcacg tcgctattca tcctttttat caggcgctgg cgctggatac
      601 acctgctatt tgctgtaatg gcacctattt gtatgattat catgcaaaaa ccgtgctgga
      661 agcggaccca atgcccgtta ataaagccct gcaactcatt gagatgctga atgaacacca
      721 cattcacggt ctgatgtatg tcgatgatgc aatggtctat gagcacccga ccgggcatgt
      781 cattcgcacg tctaactggg cgcaaaccct gccgccggaa cagcgtccga ctttcacaca
      841 agtcgcttct ctggctgaaa cgacgcaaca agttaacgcc gtatggaagt tcgccctgac
      901 gcacgatgac ctgccgcaat tgcagcattt tggtaagcat gtcgaacatg aactgggact
      961 ggagtgtgaa tggtcctggc acgatcaggt tgatattgca cacggcggca acagcaaagg
     1021 taaacgtttg acgaaatggg ttgaggcgca aggttggtcg atggaaaacg tcgtggcatt
     1081 cggcgataac tttaatgata tcagtatgct ggaagccgct ggtacaggcg tggcgatggg
     1141 caacgccgat gacgcggtaa aagcgcgcgc caacattgtg attggtgata acaccaccga
     1201 cagcattgcc cagttcattt atagccacct gatttaatca ggcggttatc gacacacttt
     1261 taatttgcgc gtacagccac aggccaggtt tgatcgccag ttcatccctg gcccacgggc
     1321 tgatacgcgc ccacagcgtt ttaccgccga cttccagctc cacttccacc tggccgttgt
     1381 cgtcataact attaacaact tttgcccgca atacgttacg aatgctggtt tgctgcggcg
     1441 gttgtaaaac cagagaaaca tcggaagcct gaatgcggat acgtagcgca gcttgcagcg
     1501 gttcgcccag cttattgacc cacaaatgct gatcgcccag cgccagcgcg gtcatcgcgt
     1561 aatgcggatg atgctcaagc accgtcactt tcagaatgct actttgttgc tctttcggca
     1621 gccacggatt catcacgcta ctgccccaca cttcctccag cgcgccaaag gctttcacct
     1681 gaccgttttc cagtaccatc actctgtctg ccagatggag gatctcatcc agcgaatggc
     1741 tgacatacaa catcggaatg ttgatttccc gtgtcagccg ttgcagataa ggcaacagtt
     1801 cgcgtttacg cggaatatcc agtgacgcca gcggttcatc cagcaacagc aattccggtg
     1861 ctgtcagcaa agcccgacca atcgccacgc gctgtttttc gcctccggac aggctgcctg
     1921 gtaaacggtc aagcaacggt tcaatgccta aaagcgccac cagcttatcg aactgatcga
     1981 ccatactttt cgacatgccg tagcgcagat tgccacgcac tttgtaatgc gggaacaacc
     2041 gcgcatcctg aaaaacatag ccaacacgac gcttttccgg cgtcaggcag ataccttttt
     2101 cggcatcatt tagtacccgc ccattgagga caatccgccc tttttgcggg cgcgtcagtc
     2161 cactgatggc gttaatcagc gaagtttttc cggcaccgga gacgccaaag atagcagtga
     2221 tgccattggc gggcagcgtt tcattaatgg tcaggcaatg gttgcccaac gtctgggaaa
     2281 aattcagctc cagcatgatt agcgccccgc ccgttcacgg ctgattctgg ccagccattc
     2341 tgaaatcaac agggagatca tcgccagcgc aatagaaata atgcacagtc tcgccgcgcc
     2401 actttccccg ccgggggtct ggatcagggt atacatggca gaaggaatgg ttcgcgtttc
     2461 gctaggaatg ttcgacacaa aggtgatggt tgcaccaaac tcaccgagag aacgagcaaa
     2521 agccagtacc gtaccaacaa taattcccgg taaggtcagc ggtaacgtga tagtaaagaa
     2581 aacgcgccag cgcccggcac ctagtgttct tgcggcctgt tccagtttga catcaacccc
     2641 ttccagcgcc agacgaattg cccgcaccat cagcggaaac gacatgacgg cggcagcgag
     2701 aaccgcgccg cgccagctaa aggcgaaggt aataccaaac cagtcataca gacgttcacc
     2761 gataaatccg cgccgtccca tcgaaactaa taataagtaa ccgacgacca cgggcggtaa
     2821 caccagcggt agatgcagta cgctgtcgag cagagctttg cccggaaacg tgcaacgcac
     2881 cagtaaccag gcaaaaaaga tcccaaacgg caggctaaac agcacagcca gggaagaaac
     2941 tttcaggctt aataaaactg cctgccattc tggatcggtc agtatcatta cttggttgta
     3001 aatccgtaac gtttaaagat ttccgctgcc tgcggtccct tcagataatc ataaaatgct
     3061 ttcactgtcg cattgttatg cccttccaca acagcaaccg gatattccac ttttttatgt
     3121 gaatcttccg ggaaggtggc aaccactttt acccctttgc tggcaactgc gtcagaaccg
     3181 tagacaatgc ccagaggcgc ttcgttacgt tcgaccagcg ccagcgcccc acgaacatct
     3241 tccgctgggg ccagtttcgg agagagcgta tcccatgcgc ccagtttttg cagtgcttct
     3301 tttgcataaa tgccagcggg aacatgttcc ggatcgccaa ccgccaggcg accgccattc
     3361 agcagcgaag tccagttagt tttgctgtcg atggtgaaat ctttctgctc gctggctttc
     3421 ggtgctacaa cgaccaggct attgccgagc agtgtctgac gcgtagcagt atcgatcgct
     3481 tttttatcaa ccgcataatc catccatttc tgatcggcag aaataaacag atccgcaggg
     3541 gcacccgctt caatctgacg ggcgagagta gatgacgaag cgaaagaaga aaccacatcc
     3601 acgcctttct cttttttata ctgcgtagca atgtcctgca ttgcgttagt cagtgatgcg
     3661 gcggcgaaca ccgtgatttt cccttcatct gccagtgcat tgccagcaac tgcgaaagag
     3721 agtgccgccc cggcaaacaa gttcaaccat ttacgagcca ttggtaaccc cttaatgtaa
     3781 cgttatgtag gcgacaatat aacgactaac tcggaatttt cccagcagtt attgctaacc
     3841 tttgtgaggt agataagaaa aaatatcggc aggaaaagca ggaagttgag aaaaagaaaa
     3901 tgcccgacta agcgggcact cagggaatca atgattttgt ccgggctggt cttttttacc
     3961 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     4021 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     4081 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     4141 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     4201 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     4261 cgcgccgcat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     4321 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     4381 gtgagcatat tctggtcgag cgcgcaacag gcggtaaagg tggcggaggc gcagtactga
     4441 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4501 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4561 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4621 atcatgatga cgttcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4681 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4741 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4801 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4861 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4921 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4981 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     5041 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     5101 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     5161 tttgtcggtt cgaatggaag tggaaagtcg gcgctggccc gcgcgctggc gggggaactt
     5221 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     5281 caattgcaaa agctcgtcag cgacgaatgg caacggaata acaccgatat gctcggccct
     5341 agcgaagatt acaccggacg cactacggct gagattattc aggatgaagt aaaggatgca
     5401 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     5461 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5521 cctgacttgt tgattcttga tgagccgttc gatggcctgg atgttgcctc acgtcagcag
     5581 ctggctgagc gactcgcctc gttacatcag tccggtatta ctttggtact ggtgctcaat
     5641 cgcttcgata agatcccgga atttgtccag tttgctggcg tgctggcgga ttgtacgtta
     5701 gcggaaactg gcgctaaaga ggaactgctc cagcaagcac tcgtcgcgca acttgcgcat
     5761 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5821 ttacccgcca acgaatcgcg cattgtgctg aacaatggcg tggtttctta taatgatcgc
     5881 cccattctta ataaccttag ctggcaggtg aatccaggcg aacactggca aattgtcggg
     5941 ccaaatggcg cgggaaaatc gacgttatta agcctgatta ctggcgatca tccgcaaggt
     6001 tacagcaacg atttgacgct tttcggccga cgtcgcggca gcagcgaaac catctgggac
     6061 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     6121 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggtcgtt
     6181 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacac
     6241 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     6301 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     6361 ccgctcaatc gccaacttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     6421 caattgttgt ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6481 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6541 agtgctgacc ttgccggagg cggcctaagc gcactctccg gccaacggtt cgacgcatgc
     6601 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6661 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6721 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accacaagcc
     6781 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6841 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6901 gtaagcgcag cgtactgcct gttatcgagc gtttaggcgg caaacatcca acgtttgttg
     6961 aaggcgatat tcgtaacgaa gcgttgatga ccgagatcct gcacgatcac gctatcgaca
     7021 ccgtgatcca cttcgccggg ctgaaagccg tcgccgaatc ggtacaaaaa ccgctggaat
     7081 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     7141 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc caaattccat
     7201 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     7261 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     7321 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     7381 ttccgaataa cctgatgcca tacatcgccc aagttgctgt aggccgtcgc gactcgctgg
     7441 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7501 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7561 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7621 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7681 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7741 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7801 atcccgatta aggaacgacc atgacgcaat ttaatctcgt tgatcatcca catcgccgct
     7861 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7921 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7981 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     8041 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     8101 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     8161 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     8221 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     8281 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     8341 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     8401 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     8461 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8521 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8581 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8641 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8701 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8761 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8821 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8881 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8941 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     9001 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     9061 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     9121 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     9181 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     9241 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     9301 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     9361 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     9421 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9481 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9541 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9601 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9661 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9721 aaggcgacct gaaacgtatg ggcgagttga tggccgagtc tcatgcctct atgcgcgatg
     9781 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gctgtgattg
     9841 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9901 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9961 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
    10021 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
    10081 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttccg cccgtattcc
    10141 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagtccgg aatgctatca
    10201 ggatcaggct gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
    10261 tcgctatacc tttgacggtg aaaccgtgac gctttcgcca agccagggcg ttaaccagct
    10321 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
    10381 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
    10441 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10501 agttgataaa ccttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10561 gcagtctgac gtgcgcaatc acaagttgca gattctggcg gacgaatatc ttccggttga
    10621 tgaaggcggc attccgcacg acggcttgaa atctgtcgcc ggaacgtctt ttgatttccg
    10681 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10741 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10801 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctccgg ctcttcaatt
    10861 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10921 gcaagggctg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10981 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    11041 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    11101 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    11161 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    11221 tattatcatt aaaagatgat ttgaggagta agcatatggc tgtaactaag ctggttctgg
    11281 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    11341 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    11401 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    11461 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11521 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aactgctgaa aagtatggcg
    11581 acgagcaggt gaaacagtgg cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag
    11641 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagaattgc
    11701 cgctgacgga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacca
    11761 ttctgccgcg tatgaagagc ggtgagcgcg taatcatcgc cgcacacggt aactccctgc
    11821 gtgcgctggt gaaatatctt gataacatga gcgaagaaga gattcttgag ctgaatatcc
    11881 cgactggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11941 atctgggtaa tgctgacgag atcgcggcga aagcagcagc cgttgcgaac cagggtaaag
    12001 cgaagtaaac gtcattcgtt taaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    12061 tgtttgtcgt tgctaacaat ggagcacatt gcctgatgcg acgctgcgcg tcttatcagg
    12121 cctgtggtga ttcatcggat acgccactct gacggcgcat ccgacaatta aaccttaccc
    12181 gcgacgcgct tttactgcat tcaccagttg acgtaacaga gcatcggtat cttcccagcc
    12241 aatgcaggca tcggtgatgc tcttaccgta ggccagcggc tccccgctat cgaggctctg
    12301 attgccttcc accagatggc tttccaccat cacgccaata atggcctttt cgccaccggc
    12361 aatctgctgg caaacgtcag cacaaacatc catctgcttt ttgaattgtt tggacgaatt
    12421 agcatggctg aaatctatca tcacctgagc tggcagacct gctttgttca gcccttcttt
    12481 cacttcagca acgtgcttcg cgctgtagtt aggctcttta ccgccgcgca gaatgatatg
    12541 gcaatcgccg ttaccactgg tattcacaat cgccgaatgc ccccatttag ttacggacag
    12601 gaagcagtgc ggcgcaccgg cggcattaat ggcatcgata gccactttaa tcgtaccgtc
    12661 tgtgccattt ttaaaaccta ccggacaaga aagaccagac gccagttcgc ggtgcacctg
    12721 cgattccgtg gtacgtgcac caattgcgcc ccagctcatc aggtcagcga gatactgagg
    12781 agtgatcata tcgagaaact cacccgccgc tggcagaccg ctgtcgttaa tatcaagcag
    12841 caatttacgg gctatacgca gaccgtcgtt gatctggaag ctgttatcca tatgcggatc
    12901 gttaatcaac cctttccagc ccaccgtggt acgcggcttt tcaaaataga cgcgcattac
    12961 gatttccagc tcatctttca gctcttcacg cagcgccagc aagcgagtgg catactcttt
    13021 agccgcgaca ggatcatgaa ttgagcacgg gccaatcacc accaacaggc gatcatcatt
    13081 acctttcagg atcttatgga tcgcttttcg ggcatgggca accgtattcg cggcattttc
    13141 agttgcgggg aatttttcca gcaatgcgac aggaggaagt aactctttga tttctttgat
    13201 gcgtaaatcg tcgttctgat aattcatgtc tgttccagtg ttgccatact tatcttagtg
    13261 aatgcaatac ttccaatcta tatcttccgt cagaatgtgt aaacggggtt ttacactatg
    13321 aacggattaa tcctggaatt agcaagaaaa acgccagatt gtcgcgaaaa acgagatctc
    13381 tcctacaatt tctaactgta actcctttgt ttgttaatta tttcaagatt ctctgctgcg
    13441 tttcataaac tggctgaaat cttaaaccaa tgccttatat tcacctgcaa atgcactgtt
    13501 ggaagaggtt atccgacata acgaccataa caggagcatc ctatgaaaat gacaaaactg
    13561 gccacattat ttctgactgc cactctaagc cttgccagcg gtgccgcact ggctgccgat
    13621 agcggagcgc aaactaataa cggccaggca aacgccgcag ctgatgcggg ccaggtagcc
    13681 cctgacgccc gtgaaaatgt cgcgccaaat aacgtcgaca ataacggggt aaataccggt
    13741 tctggcggca caatgctgca ttcggatggt tcttcaatga acaatgacgg aatgaccaaa
    13801 gatgaagagc acaagaacac catgtgcaaa gatggtcgat gtccggatat caataaaaag
    13861 gtacaaaccg gtgatggcat caataatgat gtcgatacca aaaccgacgg taccacgcag
    13921 taaccctgca aaatagccaa taatgggaga gcggagtctc tcccttttaa tttttcactt
    13981 ctaaaagcag tatgatgtca taaagttaag tgagaataat taagggatga ctcgtcatgg
    14041 cgcactcaca ctcacacacg tcctcacacc tgccagaaga taataatgct cgtcgcttgt
    14101 tgtatgcttt cggcgtgact gccgggttta tgctggtaga agtcgttggt ggttttcttt
    14161 ctggttctct ggcattgctg gccgatgcgg gtcatatgtt gaccgatact gccgccctgc
    14221 ttttcgccct actcgccgtg caattttcac gtcgtcctcc caccattcgc cacactttcg
    14281 gttggctaag actgaccacg ctcgcggcgt ttgtgaatgc catcgccctc gtggtaatta
    14341 ccattttgat tgtctgggag gcgatagaac gcttccgcac gccgcgtccg gtcgagggag
    14401 gcatgatgat ggcaattgcc gtggctgggt tgctggcaaa tatactttct ttctggttac
    14461 ttcatcacgg cagcgaagag aaaaacctca acgtacgagc agcggcactg catgtgctcg
    14521 gggacctgct gggttcggtt ggggctatta ttgccgcgtt gattattatc tggactggct
    14581 ggacacctgc tgaccccatt ctctcgatac tggtgtcgct tctggttctg cgtagcgcgt
    14641 ggcgactgtt gaaagatagc gtgaatgaat tacttgaagg tgcaccggta tcgctggata
    14701 tcgctgaact gaagcgtcgt atgtgccggg aaatcccgga agtccgcaat gtgcaccatg
    14761 tacatgtatg gatggtaggc gagaagccgg tgatgacgtt gcatgtgcag gtgatcccgc
    14821 cacacgatca cgatgccttg ttggatcaga tccaacacta cctgatggat cactatcaga
    14881 ttgagcacac caccattcag atggaatatc aaccttgtca taggccagac tgccacctga
    14941 atgaaggggt gtctggccat tcacatcatc accattaatg ggacagcgcg cgtgagcctc
    15001 tttcacgtgc gctgttgatc cacatccggc tgccgttgag cgcaataaag gtcaggatga
    15061 tgtactccag agacatggcg taaacgccct gaagtgcaaa aataacgacg ctaatcacgt
    15121 taataatcac ccacaacagc cagttttcca catacttacg cgtcatcaga atcattgcca
    15181 cgatagataa caccatcatg catgaatccc agaacgggaa agcgtccggt tgcagttcag
    15241 gcatcacaac ctgtaatcct aatgcttgca tgatcatgac tgccacgcgg gtcaaaaatg
    15301 caaacaccgg attgataaat accgtcatca gaccaatcga aacaacgcaa accgccaacc
    15361 agctgagtgc cttcggcaat ggcaaccagc gaattttcaa ctccgcctcg ttctgactgg
    15421 tttgtcgcga ccacgcatac cagccgtaaa tattcgcggc aaagaaaaac acctgtaata
    15481 gcaggctggc atacagctga atctgaaaga aaataatgcc aaacaaggta acgttgatca
    15541 ggccaaagaa gtagttgctg atcttctcca gactggcaag gccaatacac agcaacccgg
    15601 cgatcgtgcc taccgcttcg atccatgaga gatcataacc gcccgcccct attggtatat
    15661 gtaccaggat attctgcaca ctaaaaaaat ccatctttcc cccaaagcgt aacgttacgc
    15721 cttattattc gttatccacg tagtgtagcc gcaaaatcca gcatacgatt gagcggcacc
    15781 agcgccctct ctcgcagcct ctcatcgaca tgaacctcgt gattacttcc ttcctgttct
    15841 aatgcctctg cgatagcctg aaggtcattc atggccatcc acggacaatg cgcgcagctg
    15901 cggcaggttg cgccctcacc cgcggttggt gcttccagta actctttatc tggcaccgcc
    15961 tgctgcattt tgtagaaaat accccgatcg gttgccacaa taagcctctg atgaggcaat
    16021 gcttttgcag cagcgatcag ttgactggtg gaaccgaccg catccgccat atcgacaata
    16081 gcttgtggtg attctggatg caccagtatg gcagcatccg ggtattcttc ttgcaagcgg
    16141 gttaacgcct gagtcttaaa ttcatcatgc acaatgcagg caccctgcca gcataaaatg
    16201 tctccacccg tctgtttttg cacgtaacgc cccagatgtt tgtcgggtgc ccagatgatt
    16261 ttttcaccca aactatcaag atgatcaata agttcgacgg caatgcttga agttaccacc
    16321 caatctgcgc gcgcttttac cgcagcagaa gtgttggcgt agacgacgac agtacgatcg
    16381 ggatgggcat cgtaaaatgc gttaaattct tcaacagggc agccgagatc cagtgaacac
    16441 tcaacctgaa gtgtcggcat cagaattgtt ttttccggac tgagaatttt ggcggtttct
    16501 cccataaatc tcaccccagc gactaacaaa gtagaagcgg gatgctttgc accgaagcgc
    16561 gccatttcca gagaatcaga aatacagcca ccggtttctt ctgccagttg ttgaatttcg
    16621 ggatcggtat agtagtgggc aaccatcacc gcattacgtt cttttagcaa acgttttatc
    16681 ttctcgcggt aatacgcttt ttcatcaatg cttaacggcg tcggcttcgg ggggaaagga
    16741 taaatcgccg tgtctggatc aaacattacg ctcatcttac catctcgttt tacaggctta
    16801 acgttaaaac cgacattagc gtaacattcg ctgttatcgg tgtaatttgt tttatatgct
    16861 aaacaagata gtcgaatggt ggggaaaagt cactcgaatt acgcctaatt ccaccgacgt
    16921 agagttgctt gcacagtaag ctctgacgat gtcatcaggt ttacaaagtg aaattgagga
    16981 tgcctg
//
LOCUS       DAJVRL010000085        16390 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVRL010000085 DAJVRL010000000
VERSION     DAJVRL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.085"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(90..2066)
                     /gene="speA"
                     /locus_tag="OQN02_002895"
     CDS             complement(90..2066)
                     /gene="speA"
                     /locus_tag="OQN02_002895"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1643615.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="OQN02_002896"
     CDS             complement(2075..2206)
                     /gene="yqgB"
                     /locus_tag="OQN02_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1643616.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2342..2557
                     /locus_tag="OQN02_002897"
     CDS             2342..2557
                     /locus_tag="OQN02_002897"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643617.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2861..4015
                     /gene="metK"
                     /locus_tag="OQN02_002898"
     CDS             2861..4015
                     /gene="metK"
                     /locus_tag="OQN02_002898"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1643618.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4452..5846
                     /gene="galP"
                     /locus_tag="OQN02_002899"
     CDS             4452..5846
                     /gene="galP"
                     /locus_tag="OQN02_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1643619.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5923..6420
                     /locus_tag="OQN02_002900"
     CDS             5923..6420
                     /locus_tag="OQN02_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1643620.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6515..7222
                     /gene="endA"
                     /locus_tag="OQN02_002901"
     CDS             6515..7222
                     /gene="endA"
                     /locus_tag="OQN02_002901"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1643621.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7302..8033
                     /gene="rsmE"
                     /locus_tag="OQN02_002902"
     CDS             7302..8033
                     /gene="rsmE"
                     /locus_tag="OQN02_002902"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1643622.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8046..8996
                     /gene="gshB"
                     /locus_tag="OQN02_002903"
     CDS             8046..8996
                     /gene="gshB"
                     /locus_tag="OQN02_002903"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1643623.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9105..9668
                     /locus_tag="OQN02_002904"
     CDS             9105..9668
                     /locus_tag="OQN02_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1643624.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9668..10084
                     /gene="ruvX"
                     /locus_tag="OQN02_002905"
     CDS             9668..10084
                     /gene="ruvX"
                     /locus_tag="OQN02_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1643625.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10260..11240)
                     /locus_tag="OQN02_002906"
     CDS             complement(10260..11240)
                     /locus_tag="OQN02_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1643626.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11258..11962
                     /gene="yggS"
                     /locus_tag="OQN02_002907"
     CDS             11258..11962
                     /gene="yggS"
                     /locus_tag="OQN02_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1643627.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11980..12546
                     /gene="yggT"
                     /locus_tag="OQN02_002908"
     CDS             11980..12546
                     /gene="yggT"
                     /locus_tag="OQN02_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1643628.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12543..12833
                     /locus_tag="OQN02_002909"
     CDS             12543..12833
                     /locus_tag="OQN02_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1643629.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12841..13434
                     /locus_tag="OQN02_002910"
     CDS             12841..13434
                     /locus_tag="OQN02_002910"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1643630.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13427..14563
                     /gene="hemW"
                     /locus_tag="OQN02_002911"
     CDS             13427..14563
                     /gene="hemW"
                     /locus_tag="OQN02_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1643631.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14877..15840
                     /locus_tag="OQN02_002912"
                     /pseudo
     CDS             14877..15840
                     /locus_tag="OQN02_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15885..16388
                     /locus_tag="OQN02_002913"
     CDS             15885..16388
                     /locus_tag="OQN02_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCS1643632.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4061 a         4220 c         4126 g         3983 t
ORIGIN      
        1 aaaagcagct agattatttt taagaatata taaaaagaaa gcgggccgat ttacgtaaat
       61 aaatcgaccc gccagcggaa taatcttaat tattcatctt caagataagt ataaccgtac
      121 aaacctgcct cgaactcttc aaggaactgt tgttgcagtt cagcatcaag atcggttttc
      181 ttcacttgat cgcggaactg ggttaacagc gttttcggat cgagctgtac atattgcagc
      241 atgtccgcca cggtatcgcc ttcgtcagac agttctactt ctacgctacc gtcagggaac
      301 acgaacacgt caactgcttc ggtatcaccg aacaggttgt gcatattgcc aaggatctcc
      361 tgatatgcac cgaccataaa gaaaccgagc atcggcggat tctctggatc gtactccggc
      421 attggcattg tcgtggcaat accatcacca tcaatatagt ggtcgatagc accgtcagag
      481 tcacaggtaa tatccagcag cacagcgcga cgttccggca cttgatccag cccttccagc
      541 ggtagaaccg ggaacaactg gtcgatcccc catgcgtccg gcattgactg gaacagcgag
      601 aaattgacgt acattttgtc cgccatacgt tcctgcagtt cgtcgattat cggacgatgc
      661 gcacggtttt gcggatccag ttgcttctgc acttcatggc acatgctcag atagagttgt
      721 tctgcccatg cacgttcttg caggctaaag gtgccggaag agtagccgat atgaatgtcg
      781 tgcagatcca tctggctgtc gtgtaaccat tcacgcaaag aacggcgagt tcccggttcg
      841 tgcatttcct gccaggtttc ccacatgctt tgcagcgcac gcggcgcatc ttctacgggc
      901 gcggtcggca ccgtatattc gttacgttcc acgccgatga tattcgacac cagcacggta
      961 tggtgcgcag tcaccgcacg gcccgattcg gtgattaccg tcggatgcgg cagaccattt
     1021 tcttcacacg catcgccaat cgcccagatg atgttgttgg cgtattcatt gaggccatag
     1081 tttacggagc agtcagactg cgaacgggtg ccttcataat ccacgcccag accgccgccg
     1141 acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat
     1201 tcacgaacgc ctgtcgcgat atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc
     1261 agtagttgca ggctgtcgag acgcccggct tcacgcaggg tttcaaccag ttgcagtacc
     1321 tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg aggactgcca tttacccgag
     1381 ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa cgacattcag acgttctgct
     1441 tcatccagca caatggcgat ttctgacatc ttctcaatga ccagatagac cttgtgcccc
     1501 atcttctcgc caattaatgc caagcggata tattcgcggt ctttataacc gttgcagacg
     1561 atgacgctac gagtcatgcc agcatgtgcc agcactgcca tcaactcggc tttggaaccg
     1621 gcttccagac ccagcggttc acccgaatga atcagggact cgatcacgcg gcggtgctgg
     1681 ttaactttga tcggataaac aaggaagtaa tcgccgttat agccgtagga ttccctcgca
     1741 cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac
     1801 agtgcaggca gacgctgacc ctgtgcttca cgagttttca ctaactgcgc gagatcgacg
     1861 cgagcttccg ggacgtccgg gtccgggcac acgctgatgt ggcccagctc gttaacgtca
     1921 tagtagttat tgccccacca ggcaatattg taagtacgca gcatcttgct ggcttcctgg
     1981 gagctcattg caacctcctg catggagcgt agtacaccgt gttcgcccgc tgacgaaggc
     2041 aaacccatag acatgtcgtc agacatagcg aacctcaaat tattttatta agtgtaaaac
     2101 agttaacgac tgtcgcagcc tggaactgcg ataacaaccc ataagcacat ggattttcca
     2161 gcagtgtatg ctgacgctcc gactgcgcaa ccggtttctt tttcataaca ttattaagca
     2221 cataaccgaa cgtaagtgtg acagttcggc gaaaccacga gaaaactctt gtttttacaa
     2281 gagcgccctt gttcagtcct cagtaactgt aaccagctct tgaatcctga gaagcgccga
     2341 gatgggtata acatcggcag gtatgcaaag cagagatgca gagtgcgggg aacgagtctt
     2401 caccagaacg gtgagacagg ttaagcagca gacaacggtt cattatttcg tatcacctcc
     2461 acggccgcct gttaagacga acccacaagc caaaactctg atttcaaccc ggctggaagt
     2521 ggcaacacga aagaaacgtc gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc
     2581 cacaatggca gaaaattgca aaagataaat acgcagaatg ccggcattgt caggaaaaat
     2641 ttccagccac gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa
     2701 gaattgacct aaaatagcca tccagatgtt aatccatcca taccgattaa cactcagact
     2761 gccagtgttt ttaacctgca gagtcgtggt aggatccgct accacagaaa atccacacaa
     2821 cagtttgagc taaccaaatt ctctttaggt gatattaaat atggcaaaac acctttttac
     2881 gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaatct ctgatgccgt
     2941 tttagacgcg atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa
     3001 aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga
     3061 gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc
     3121 taactcctgt gcggttctga gcgctatcgg caaacagtct cctgacatca accagggcgt
     3181 tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag ggtctgatgt ttggctacgc
     3241 aactaatgaa accgacgtgc tgatgccagc acctatcacc tatgcacacc gtctggtaca
     3301 gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag
     3361 ccaggtgact ttccagtatg acgacggcaa aatcgttggt attgatgctg tcgtgctttc
     3421 cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat
     3481 catcaagcca attctgcccg ctgaatggct gacttctgcc accaaattct tcatcaaccc
     3541 gaccggtcgt ttcgttatcg gtggcccaat gggtgactgc ggtctgactg gccgtaaaat
     3601 tatcgttgat acctacggcg gcatggcgcg tcacggtggc ggtgcattct ctggtaaaga
     3661 tccatcaaaa gtggaccgtt ccgcagccta cgcagcacgt tatgtcgcga aaaacatcgt
     3721 tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga
     3781 accgacctcc atcatggtag aaactttcgg tactgagaaa gtgccttctg aacaactgac
     3841 tctgctggta cgtgagttct tcgacctgcg cccatacgga ctgattcaga tgctggatct
     3901 gctgcacccg atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc
     3961 gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct gccggtctga agtaatcttt
     4021 cttcacctgc gttcaaaggc cagcctcgcg ctggcctttt tcttttacct caccgcggtg
     4081 gataggcgtt cacgccgcat ccggcaaaaa aactgcccgc acaataacat cattcttcct
     4141 gatcacgttt cacggcaaat taccatcaca actgaaaccg attacaccaa ccacaccagc
     4201 aaaatatttg taatattttc atattattat tcagctttca cagttgttac atttcttttc
     4261 agtaaagtct taattgcaga tgacagcgtt taatctatga tgatataact caattatttt
     4321 catgccctta aatcataact aagataaatg ttagtgtaag cgattacact gatgtgattt
     4381 gcttcacatc tttttacgtc gtactcacct atcttaattc acaataaaaa ataactatat
     4441 tggagggcat catgcctgac gctaaaaaac aggggcggtc aaacaaggca atgacgtttt
     4501 tcgtctgctt ccttgccgct ctggcgggat tactctttgg cctggatatc ggtgtaattg
     4561 ctggcgcact gccgtttatt gcagatgaat tccagataac ttcgcacacg caagaatggg
     4621 tcgtaagctc catgatgttc ggtgcggcag tcggtgcggt gggcatcggc tggctctcct
     4681 ttaaactcgg gcgcaaaaag agcctgatga tcggcgcaat cctgtttgtt gccggttcac
     4741 tgttctctgc ggctgcgcca aacgttgaag tactgattct ttcgcgcgtt ctgctggggc
     4801 tggcggtggg tgtggcctct tataccgcac cactgtacct gtcagaaatt gcgccggaaa
     4861 aaattcgcgg cagtatgatc tcgatgtatc agttgatgat cactatcggg atcctcggtg
     4921 cttatctttc tgataccgcc ttcagctaca ccggtgcatg gcgctggatg ctgggtgtga
     4981 ttatcatccc ggcaattttg ctgctgattg gtgtcttctt cctgccagac agcccacgtt
     5041 ggtttgccgc caaacgccgt tttgttgatg ccgaacgcgt gctgctacgc ctgcgtgaca
     5101 ccagcgcgga agcgaaacgc gaactggatg aaatccgtga aagtttgcag gttaaacaga
     5161 gtggctgggc gctgtttaaa gagaatagca acttccgccg cgcggtgttc cttggcgtac
     5221 tgttacaggt aatgcagcaa ttcaccggga tgaacgtcat catgtattac gcgccgaaaa
     5281 tcttcgaact ggcgggttat accaacacca ccgagcaaat gtgggggaca gtgattgtcg
     5341 gcctgaccaa cgtacttgcc acctttatcg caatcggcct tgttgaccgc tggggacgta
     5401 aaccaacgct aacgctgggc ttcctggtga tggctgctgg catgggcgta ctcggtacaa
     5461 tgatgcatat cggtatccac tctccgtcgg cgcagtattt cgccatcgcc atgctgctga
     5521 tgtttattgt cggttttgcc atgagtgccg gtccgctgat ttgggtactg tgctccgaaa
     5581 ttcagccgct gaaaggccgc gattttggca tcacctgctc caccgccacc aactggattg
     5641 ccaacatgat cgttggcgca acgttcctga ccatgctcaa cacgctgggt aacgccaaca
     5701 ccttctgggt gtatgcggct ctgaacgtac tgtttatcct gctgacattg tggctggtac
     5761 cggaaaccaa acacgtttcg ctggaacata ttgaacgtaa tctgatgaaa ggtcgtaaac
     5821 tgcgcgaaat cggcgctcac gattaatctc ctcaggcttc ctcccgtcgc ggaggaagcc
     5881 gcctcttgca gtcatctttt cttcgctcta tcctctgccg ctatgaaaac atcccgtctc
     5941 cctatcgcca tccaacaggc cgttatgcgc cgcctgcggg aaaaactcgc ccaggccaac
     6001 ctgaagctag ggcgtaacta cccggagcca aaactctctt acacccagcg cggaacctcc
     6061 gccggaacag cctggctgga aagctatgaa atccgcctca atcccgtttt gctgttggaa
     6121 aacagtgaag cttttattga agaagtggta ccgcacgaac tggcacattt gctggtatgg
     6181 aaacatttcg gccgcgtagc gccacatggc aaagagtgga agtggatgat ggaaagcgtg
     6241 ctgggcgttc ccgcccgtcg tacgcatcag ttcgaactgc aatccgtgcg tcgcaacacc
     6301 ttcccctacc gctgtaagtg ccaggaacat cagcttaccg tacgccgcca taatcgcgta
     6361 gttcgtggcg aggccatcta tcgctgtgtt cactgcggtg aacagctggt tgcgaaataa
     6421 ccacctgaac tatcaggaac tttcctgatc tggctgattg cataccaaaa caactttcgc
     6481 tacgttgctg gctcgtttta acacggagta agtgatgtac cgttatttgt ctattgctgc
     6541 ggtggtactg agcgcagcat tttccggccc gacgttggcc gaaggtatca atagtttttc
     6601 tcaggcgaaa gccgcggcgg taaaagtcca cgctgatgcg cccggtacgt tttattgcgg
     6661 atgtaaaatt aactggcagg gcaaaaaagg cgttgttgat ctgcaatcgt gcggctatca
     6721 ggtgcgcaaa aatgaaaacc gcgccagccg cgtagagtgg gaacacgtcg ttcccgcctg
     6781 gcaattcggt caccagcgcc agtgctggca ggacggtgga cgtaaaaact gcgctaaaga
     6841 tccggtctat cgcaagatgg aaagcgatat gcataacctg cagccgtcag tcggcgaggt
     6901 gaatggcgat cgcggcaatt ttatgtacag ccagtggaat ggcggcgaag gccagtacgg
     6961 tcaatgcgcc atgaaggtcg atttcaaaga aaaagctgcc gaaccaccag cgcgtgcacg
     7021 cggtgccatt gcgcgcacct acttctatat gcgcgaccaa tacaacctga cactctctcg
     7081 ccagcaaacg cagctgttca acgcatggaa caagatgtat ccggttaccg actgggagtg
     7141 cgagcgcgat gaacgcatcg cgaaggtgca gggcaatcat aacccgtatg tgcaacgcgc
     7201 ttgccaggcg cgaaagagct aacctacact agcgggattc tttttgttaa cccctacccc
     7261 acgcgtacaa ccgcgtgggg agacgacgcg gatttttaac tatgcgtatc ccccgcattt
     7321 atcatcctga accactgacc agccattctc acatcgcgct ttgcgaagat gccgccaacc
     7381 atatcgggcg cgtactgcgc atggggccgg ggcaggcgtt gcaattgttt gacggtagca
     7441 accaggtctt tgacgccgaa attaccagcg ccagcaaaaa aagcgtggaa gtgaaggtgc
     7501 tggaaggcca gatcgacgat cgcgaatctc cgctgcatat tcacctcggt caggtgatgt
     7561 cgcgtggtga aaaaatggaa tttactatcc agaaatcgat cgaactcggt gtaagcctca
     7621 ttacgccact tttttctgag cgctgcggcg ttaaactgga tagtgaacgt ctgaacaaga
     7681 agcttcagca gtggcagaag attgcaattg ctgcctgtga gcagtgtggt cgtaaccggg
     7741 tgccggaaat ccgtccagcg atggatctgg aagcctggtg tgcagagcag gatgaaggac
     7801 tgaaactgaa tcttcacccg cgcgccagta acagcatcaa tacgttgcct ttaccggttg
     7861 aacgcgtccg cctgctgatt ggcccggaag gcggtttatc ggcagatgaa attgccatga
     7921 ctgcccgcta tcaatttact gatatcctgt tgggacctcg cgttttgcgt acagagacaa
     7981 ctgcgctcac cgccattacc gcgctacaag tacgatttgg cgatttgggc taacggagaa
     8041 gaataatgat caagctcggc atcgtgatgg accccatcgc aaacatcaac atcaagaaag
     8101 attccagttt cgctatgttg ctggaagcac agcgtcgtgg ttacgaactt cactatatgg
     8161 agatgggcga tctgtatctg atcaatggcg aagcccgcgc ccatacccgc acgctgaacg
     8221 tgaagcagaa ctacgaagag tggttttcgt tcgtcggtga acaggatctg ccgctggccg
     8281 atctcgatgt gatcctgatg cgtaaagacc cgccgtttga taccgagttt atctacgcga
     8341 cctatattct ggaacgtgcc gaagagaaag ggacgctgat cgttaacaag ccgcagagcc
     8401 tgcgcgactg taacgagaaa ctgtttaccg cctggttctc tgacttaacg ccagaaacgc
     8461 tggttacgcg caataaagcg cagctaaaag cgttctggga gaaacacagc gacatcattc
     8521 ttaagccgct ggacggtatg ggcggcgcgt cgattttccg cgtgaaagaa ggcgatccaa
     8581 acctcggcgt gattgccgaa accctgactg agcatggcac ttgctactgc atggcgcaaa
     8641 attacctgcc agccattaaa gatggcgaca aacgcgtgct ggtggtggat ggcgagccgg
     8701 taccgtactg cctggcgcgt attccgcagg ggggcgaaac ccgtggcaat ctggctgccg
     8761 gtggtcgcgg tgaacctcgt ccgctgacgg aaagtgactg gaaaatcgcc cgtcagatcg
     8821 ggccgacgct gaaagaaaaa gggctgattt ttgttggtct ggatatcatc ggcgaccgtc
     8881 tgactgaaat taacgtcacc agcccaacct gtattcgtga gattgaagca gagtttccgg
     8941 tgtcgatcac cggaatgtta atggatgcca tcgaagcacg tttacagcag cagtaaccca
     9001 ccttagcgag aaggatctcg ttgagactct gagtgacagc gcccttcttt ccacgcatac
     9061 tgggcgctgt tgcttttttg aaccaggaaa cagaacctct gacaatgaat ttacagcatc
     9121 actttcttat tgccatgcct gctctccagg atccgatttt ccgtcgttcc gtggtctaca
     9181 tttgcgaaca taataccaat ggtgcaatgg ggatcatcgt caacaagccg ctggaaaatc
     9241 tcaaaattga agggattctg gaaaagctga agatcacgcc ggagccgcgt gatgaatcaa
     9301 tccgtctgga taaaccggtt atgctcggcg gcccactggc tgaagatcgc gggtttattt
     9361 tgcatactcc gccctccaat tttgcttcca gcattcgcat ttcagacaac acggtgatga
     9421 ccacttcccg cgatgtgctg gaaacgctcg gcaccgataa acaaccgtct gacgtattgg
     9481 tggctctggg ctatgcctcc tgggaaaaag gtcaactgga acaagaaatt ctcgataacg
     9541 cgtggctaac ggccccggcg gatctgaata ttctgttcaa aacgccgatt gccggccgct
     9601 ggcgcgaggc ggcaaaactg attggtgtgg atattctcac catgcctggt gtggcaggac
     9661 acgcctgatg agtggaacct tactcgcctt cgacttcggc accaaaagca ttggcgtagc
     9721 ggtcggccaa cgcattaccg gcaccgctcg ccctttgcct gcaattaaag cgcaggacgg
     9781 tacgccggac tggaacctca tcgaacgttt actgaaagag tggcagcctg acgaaatcat
     9841 cgtcggtttg ccgctgaata tggacggcac cgaacagccg ttaaccgcca gagcgcgcaa
     9901 atttgccaac cgtattcatg gtcgtttcgg tgttgaagta aagctccatg acgagcgtct
     9961 tagcactgtg gaagcccgtt ccggtctttt tgaacagggc ggctatcggg cgctcaacaa
    10021 aggcaaagtt gactctgcct ctgcggttat tattctcgaa agctatttcg agcagggata
    10081 ttaaggcgat ctaaaagcct ggcggatagt cgttaccgcc atcttgccgg atgcggcgtg
    10141 aacgccttat ccagcacaca tctggcagcg gctgtaggtc tgataagacg cgttagcgtc
    10201 gcatcagaca tttattgcct ttataggccc tataaacttg cgcatcgggc atggcaacgt
    10261 cacaaacgcc cttcccccac ccgctgctga taactctgct gaaacgttaa catccccacc
    10321 tgctgcccgg tttgaataac atgcggtaac tggtgggttt tcccttcgcg aatcaaattc
    10381 cccaccgcgg gagtgttaat cagtaattca aacagcgcca cgcgtccttc ctgtttatcc
    10441 acttccagct tttgtgacag cactgcccgt aaactacccg caagttgatt acgtacggga
    10501 tctttttcct gcgccggaaa tgaatccacc agtcgctcaa ctgcctgcgc ggcaccacgt
    10561 gtatgtaagg ttgccagcac cagatgcccg gtttctgccg ccgtcagcgc cagacggatt
    10621 gtctcactgt cacgtagctc tccgagcaaa atcacatcag gatcttcccg caatgcagcc
    10681 cgcaaccccg atgcgaacgt catacagtgc aaaccaattt cccgctgctg gatcaaacat
    10741 cgctggctgg catagagata ttccacagga tcttccagcg tcagaatatg cgcatcggca
    10801 tgttgattaa gatagccaac catcgccgcc agcgtggtag atttgccact ccccgtagcc
    10861 cccgtcacca gaatcaggcc attctcgctc ttgagtaatt ccggcaatac cggtggtgca
    10921 ccaagctgtt cgagctgtgg acagtgcgaa ggtaacaacc gtaatgccag agaaatacct
    10981 tgccgttgcg cgaacgcact gccacgcaac cgctggtttt ccgccagcga cacggcaaaa
    11041 tccagctggc cattttccag caatattgcc cgctgatcgt catccagcca ctcccgcagt
    11101 agctcttcga cgtccggcgc atcaaacggc gcagcttcca ttctgccacg aatgcgccat
    11161 cgtgcgggcc aggcgctgca caggtgtaga tccgagacgt tatgctttac actaagggcc
    11221 acaatttctt ccatattcat actaagatcc tcggaaaatg aacgatattg cgcataacct
    11281 ggcacaggtc cgggacaaaa tctcagcggc tgcaacgcgt tgcggccgtt ctccagaaga
    11341 aattacgctg cttgcagtca gtaaaacaaa acctgcgagc gccatcgcag aagccattga
    11401 tgccgggcag cgtcaatttg gtgaaaacta cgttcaggaa ggggtagata aaattcgcca
    11461 ctttcaggaa ctgggcgtaa caggattaga atggcatttt attggcccgt tgcagtctaa
    11521 taaaagccgc ctagtggcag agcatttcga ctggtgtcat accatcgacc gtttgcgcat
    11581 tgctacccgc ctcaacgagc agcgcccggc agaacttccc cctcttaacg tactgattca
    11641 aattaatatt agtgatgaaa acagtaagtc cgggattcaa ctggcagaac tggacgagct
    11701 ggcagcggcg gtcgctgaac taccgcgttt acgtctgcgc gggctgatgg caatccctgc
    11761 gcctgagtca gaatatgtaa ggcagtttga agttgcacgc caaatggctg tagcatttgc
    11821 cggactgaaa acgcgctacc cgcatatcga cacgctctct ctgggaatgt cggacgatat
    11881 ggaagccgcc attgcggcag gtagcacgat ggttcgtatc ggcactgcaa tttttggtgc
    11941 gcgtgattac tctaaaaaat aaggaattaa aggaacgcca tgaatacgtt gactttcctg
    12001 ctttcaacgg tcattgagct gtatacaatg gtgctgttat tacgcatctg gatgcagtgg
    12061 gctcattgtg atttttacaa ccccttctca cagtttgtag tgaaggtagc gcagccaatt
    12121 atcgggccac tgcgccgcgt tattccggca atgggtccca ttgacagcgc ctcgctgctg
    12181 gttgcctata ttctcagttt tatcaaagcc attgtgctgt ttgaagtggt gaccttcctg
    12241 ccaatcatct ggattgccgg tttactgatt ctgctgaaaa ccatcggctt gctgattttc
    12301 tgggtcctgc tggtgatggc gattatgagc tgggtaagcc agggtcgtag cccgattgaa
    12361 tacgtgctga ttcagctggc cgatccgctg ctgcgcccga ttcgccgctt gctaccggca
    12421 atgggtggga ttgatttctc gccgatgatc ctcgttctgc tgctgtatgc catcaatatg
    12481 ggtgtcgcag aagtattaca ggcaaccgga aacatgctgc tgccggggct gtggatggcg
    12541 ttatgagtgc cgtaacagtt aatgatgacg gtctggtttt acagctctat attcagccga
    12601 aagccagccg tgattctatt gtcggtttac atggcgacga agttaaagtc gccattaccg
    12661 cgccgccggt tgacggccag gccaacagtc atctggtgaa gtttctcggt aagcaattcc
    12721 gggttgccaa aagccaggtg gtgattgaaa aaggcgaact tggccgccac aaacaaatta
    12781 aaatcattaa tccgcaacaa atcccgccag aaatcgcggc gttaattaat taggtatcct
    12841 atgcaaaaag ttgtcctcgc aaccggcaat gccggtaaag tgcgtgagct ggcgtcgctg
    12901 cttagcgact ttggtcttga tatcgtggcc caaacagacc tcggcgttga ttccgctgaa
    12961 gaaaccggcc taacctttat cgaaaacgcg attctgaaag cgcgccatgc ggcaaaagtg
    13021 accggtttac cggcaattgc cgacgactct ggtctggcgg tagatgcgct gggtggcgcg
    13081 ccgggtattt actccgcgcg ttattccggt gaagacgcga ccgatcaaaa gaatctgcaa
    13141 aaactgttgg aaacactgaa agacgtaccg gacgaccaac gtcaggcgcg tttccactgc
    13201 gtgctggtat atctgcgtca cgcggaagat ccgactccgc tggtatgcca cggcagctgg
    13261 ccgggcgtga ttactcgcga accagcgggc actggtggct ttggttatga tccaatcttc
    13321 ttcgtacctt ccgaagggaa aaccgccgcc gaactgaccc gcgaagaaaa gagcgccatt
    13381 tcccaccgtg gtcaggcgtt gaaactgctg ctggacgctt tacgtaatgg ttaaattacc
    13441 gccgctgagt ctctacattc acatcccgtg gtgcgtgcag aaatgcccgt actgcgattt
    13501 caactctcac gcgttgaaag gagaagtgcc gcacgacgat tatgttcagc atctgctttg
    13561 cgatctggac aatgatgtgg cttacgctca gggccgtgaa gtaaagacaa tctttattgg
    13621 cggtggtacg ccgagcctgc tttccggccc ggcgatgcaa acgctgctag acggcgtgcg
    13681 tgcgcgtttg ccgctggcag cggatgcaga aattactatg gaagcgaacc caggcacggt
    13741 agaagcggat cgctttgtcg attatcagcg tgctggtgta aaccgcatct ctattggtgt
    13801 gcagagtttt agcgaagaaa agctgaaacg acttgggcgt attcatggcc cgcaagaagc
    13861 gaaacgagcg gcaaagctgg cgagcggttt agggttacgt agctttaacc ttgatttgat
    13921 gcatggacta ccggatcaat cactggaaga ggcgcttggc gatctacgcc aggccattga
    13981 actgaatccg ccgcatcttt cctggtatca actgactatc gaacctaata cgctgtttgg
    14041 ctcgcgaccg ccggtgctgc cggacgatga cgcgctgtgg gatattttcg aacaggggca
    14101 tcagttatta accgcagcgg gttatcagca atatgaaact tccgcttacg ccaaacccgg
    14161 ttatcagtgc cagcacaatc tcaactactg gcgctttggt gactacatcg gtattggctg
    14221 cggcgcgcac ggcaaagtga ccttcccgga tgggcgcatt ctgcgtacca ctaaaacgcg
    14281 tcatcctcgt ggttttatgc aaggaaggta tctggaaagc cagcgtgatg tcgaagccgc
    14341 agataagccg tttgagttct ttatgaatcg cttccgtctg ctggaggccg cgccgcgcgt
    14401 ggagtttatt gcgtataccg ggctttgcga agatgtgatt cgcccacagt tagacgaggc
    14461 gattgcccag ggttatctca ccgaatgtgc ggattactgg cagataacgg aacatggtaa
    14521 attattctta aactcacttc ttgaattatt cctcgctgag taatcattaa tgcgcctgcg
    14581 ggcgcattaa tcacttcttt tatccccccg ttaattaacc tcggcagaaa tatatttctt
    14641 actgaataaa tatcgacgac atttattata tatatttagt gaatatttac taatcattca
    14701 ttgattataa atatgtacaa cctggcgcac aaatttaata tttaatttct gactttattt
    14761 caagttgtga taaacataga caagttgtca gacaggttaa ccaatttgtc gcacaaatca
    14821 ctgataagca caattagata tcaaccaatt tccatccttc aacagggagt ttttttatga
    14881 aaatgaggtc atttacccgc tcattagtct gcgcatcctt gctggctctg gttagcactg
    14941 gcgtaaacgc cgcagaaaaa gtggcgttaa agttagcaca caacctggaa agaagtcatg
    15001 ttgtacatca gagttttgaa gagctggcga aagaggtaaa acagctgtca aaaggtaata
    15061 tggtgatccg tatttatccc agcagccaga tgggtaatgc gcgagaaact atggaattgt
    15121 tgcaaaatgg cgcactggat atgaccaaag gttccgccag tgatctggag tcttttgata
    15181 atatttatgc tatctataat ttgccgttct tattaagttg tttttggtga agttggtaaa
    15241 gaaattatgg actcaacaaa agacaaaggc ttttttgctt tatcagctta tgtagcgggt
    15301 actcgcagtt tctatgcgaa aaaaccgatt acaaagccag aagatcttaa aggactgaaa
    15361 attcgtgttc agccaagtcc gaccaccatc aaaatgattg aactgatggg tggttcaccc
    15421 actccaattt catttggcga agtttatact gcgatgcagc aaggtgtagt agatggcgca
    15481 gaaaataacg tgccttcctg gatgcagact cgacatattg aaattgccaa agttttttct
    15541 gaagatgaac acgcctcaat tcctgacttt ttggttatct ccaccaaaac atggaataaa
    15601 ttaaccccag aacagcaaca aatcctcgaa acagcggcga agaaatccga agcttatcaa
    15661 caaaaattgt gggaaaaaat tgacgctgac acacgtgctc aggcgaaagc tatggggggt
    15721 gaaattgtaa aagtggataa agcgccattc cgtaaagccg tgcaacctct gtttgacgac
    15781 ttcaaaaaag atccaaaaca agctgctttg ttagagaaat tcgacaacgc tgctcaataa
    15841 tattccgggc cagcctctgg ccctgatttt ccaggatgct tacaatgata tttaaccgcc
    15901 tgaaacttgc ggtagaccgc gtcattgccg cgttttctgt cgccgtaatg cttgctctgg
    15961 ttgtatgcgt agtctggcaa gtatttagcc gctacgtact taaccagcca agtacattaa
    16021 ccgatgagct ggcacgcttt ttgatgatct gggtaggatt attaggtgct gcatataccg
    16081 tcggcgcgca gcgacatctc tctattgatt tatttgctct cgccctgaat aagcgcaaac
    16141 aattacttct tagtatagtc attaacgttc tcattttagg ttttgccggt tcggtcattg
    16201 tgactggtgg cctgaaatta attgataaaa cactggcaac atcacaggta tctgccgcta
    16261 tgcaaatacc gatgggatac gtatatatca ttctgccatt aagtggagtg gtaatgatgt
    16321 tttatgcctt gtgttttatt aatcaaagca ttcaacaact gaaacaacct gcgcaggagg
    16381 ccagttaatg
//
LOCUS       DAJVRL010000086        15528 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVRL010000086 DAJVRL010000000
VERSION     DAJVRL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.086"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OQN02_002914"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OQN02_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCS1643633.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OQN02_002915"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OQN02_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCS1643634.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OQN02_002916"
     CDS             complement(941..1408)
                     /locus_tag="OQN02_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1643635.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1789..2100
                     /gene="rpsJ"
                     /locus_tag="OQN02_002917"
     CDS             1789..2100
                     /gene="rpsJ"
                     /locus_tag="OQN02_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1643636.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2133..2762
                     /gene="rplC"
                     /locus_tag="OQN02_002918"
     CDS             2133..2762
                     /gene="rplC"
                     /locus_tag="OQN02_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1643637.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2773..3378
                     /gene="rplD"
                     /locus_tag="OQN02_002919"
     CDS             2773..3378
                     /gene="rplD"
                     /locus_tag="OQN02_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1643638.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3375..3677
                     /gene="rplW"
                     /locus_tag="OQN02_002920"
     CDS             3375..3677
                     /gene="rplW"
                     /locus_tag="OQN02_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1643639.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3695..4516
                     /gene="rplB"
                     /locus_tag="OQN02_002921"
     CDS             3695..4516
                     /gene="rplB"
                     /locus_tag="OQN02_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1643640.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4533..4811
                     /gene="rpsS"
                     /locus_tag="OQN02_002922"
     CDS             4533..4811
                     /gene="rpsS"
                     /locus_tag="OQN02_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1643641.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4826..5158
                     /gene="rplV"
                     /locus_tag="OQN02_002923"
     CDS             4826..5158
                     /gene="rplV"
                     /locus_tag="OQN02_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1643642.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5176..5877
                     /gene="rpsC"
                     /locus_tag="OQN02_002924"
     CDS             5176..5877
                     /gene="rpsC"
                     /locus_tag="OQN02_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1643643.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5890..6300
                     /gene="rplP"
                     /locus_tag="OQN02_002925"
     CDS             5890..6300
                     /gene="rplP"
                     /locus_tag="OQN02_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1643644.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6300..6491
                     /gene="rpmC"
                     /locus_tag="OQN02_002926"
     CDS             6300..6491
                     /gene="rpmC"
                     /locus_tag="OQN02_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1643645.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6491..6745
                     /gene="rpsQ"
                     /locus_tag="OQN02_002927"
     CDS             6491..6745
                     /gene="rpsQ"
                     /locus_tag="OQN02_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1643646.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6910..7281
                     /gene="rplN"
                     /locus_tag="OQN02_002928"
     CDS             6910..7281
                     /gene="rplN"
                     /locus_tag="OQN02_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1643647.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7292..7606
                     /gene="rplX"
                     /locus_tag="OQN02_002929"
     CDS             7292..7606
                     /gene="rplX"
                     /locus_tag="OQN02_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1643648.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7621..8160
                     /gene="rplE"
                     /locus_tag="OQN02_002930"
     CDS             7621..8160
                     /gene="rplE"
                     /locus_tag="OQN02_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1643649.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8175..8480
                     /gene="rpsN"
                     /locus_tag="OQN02_002931"
     CDS             8175..8480
                     /gene="rpsN"
                     /locus_tag="OQN02_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1643650.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8514..8906
                     /gene="rpsH"
                     /locus_tag="OQN02_002932"
     CDS             8514..8906
                     /gene="rpsH"
                     /locus_tag="OQN02_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1643651.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8919..9452
                     /gene="rplF"
                     /locus_tag="OQN02_002933"
     CDS             8919..9452
                     /gene="rplF"
                     /locus_tag="OQN02_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1643652.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9462..9815
                     /gene="rplR"
                     /locus_tag="OQN02_002934"
     CDS             9462..9815
                     /gene="rplR"
                     /locus_tag="OQN02_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1643653.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9830..10333
                     /gene="rpsE"
                     /locus_tag="OQN02_002935"
     CDS             9830..10333
                     /gene="rpsE"
                     /locus_tag="OQN02_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1643654.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10337..10516
                     /gene="rpmD"
                     /locus_tag="OQN02_002936"
     CDS             10337..10516
                     /gene="rpmD"
                     /locus_tag="OQN02_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1643655.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10520..10954
                     /gene="rplO"
                     /locus_tag="OQN02_002937"
     CDS             10520..10954
                     /gene="rplO"
                     /locus_tag="OQN02_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1643656.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10962..12293
                     /gene="secY"
                     /locus_tag="OQN02_002938"
     CDS             10962..12293
                     /gene="secY"
                     /locus_tag="OQN02_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1643657.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12325..12441
                     /gene="rpmJ"
                     /locus_tag="OQN02_002939"
     CDS             12325..12441
                     /gene="rpmJ"
                     /locus_tag="OQN02_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1643658.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12588..12944
                     /gene="rpsM"
                     /locus_tag="OQN02_002940"
     CDS             12588..12944
                     /gene="rpsM"
                     /locus_tag="OQN02_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1643659.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12961..13350
                     /gene="rpsK"
                     /locus_tag="OQN02_002941"
     CDS             12961..13350
                     /gene="rpsK"
                     /locus_tag="OQN02_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1643660.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13384..14004
                     /gene="rpsD"
                     /locus_tag="OQN02_002942"
     CDS             13384..14004
                     /gene="rpsD"
                     /locus_tag="OQN02_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1643661.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14030..15019
                     /gene="rpoA"
                     /locus_tag="OQN02_002943"
     CDS             14030..15019
                     /gene="rpoA"
                     /locus_tag="OQN02_002943"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1643662.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15060..15443
                     /gene="rplQ"
                     /locus_tag="OQN02_002944"
     CDS             15060..15443
                     /gene="rplQ"
                     /locus_tag="OQN02_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1643663.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4008 a         3645 c         4130 g         3745 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt actattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccaatcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agagcgctga tttgttaacc tgattccggt
     1501 gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat gcagctcgca atctgcgcgt
     1561 tgcgattaat ttctacccga tgcttgcgtc aggctcagat ttgcgtataa tgcgcgggct
     1621 tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag ccgaatttgg ctacctaaca
     1681 atgctcccaa tcggggagct acgtaagaac ggttacactc tcccatcaat cgtaatgggt
     1741 ctgaggagta atcattttcg tttataaaat aattggagct ctggtctcat gcagaaccaa
     1801 agaatccgta tccgcctgaa agcgtttgat catcgtctga tcgatcaagc aaccgcggaa
     1861 atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg gtccgatccc gctgccgaca
     1921 cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca acaaagacgc gcgcgatcag
     1981 tacgaaatcc gtactcactt gcgtctggtt gacatcgttg agccaaccga gaaaaccgtt
     2041 gatgctctga tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag cctgggttaa
     2101 tcaggtcatt gagcgattga gaggttgaaa caatgattgg tttagtcggt aaaaaagtgg
     2161 gtatgacccg tatcttcaca gaagacggcg tttctatccc agtaaccgta atcgaagttg
     2221 aagcaaaccg cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg
     2281 tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta
     2341 aagctggcgt agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt
     2401 tcactgtagg tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa
     2461 ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc
     2521 aggacgctac tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc
     2581 agactccggg caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg
     2641 taaccgttca gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta
     2701 aaggtgctgt cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt
     2761 aaggagatag caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa
     2821 actaccttcg gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca
     2881 gctggtgctc gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt
     2941 aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg
     3001 atctggcgtt ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt
     3061 aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat
     3121 cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag
     3181 aaactgaaag acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac
     3241 ctgttcctgg ctgcgcgcaa cctgcagaag gttgacgtac gcgatgcaac tggtatcgac
     3301 ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt
     3361 gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg
     3421 tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcga
     3481 aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg
     3541 aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc
     3601 gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg
     3661 ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac
     3721 atctccgggt cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc
     3781 ttttgctccg ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat
     3841 caccactcgt catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg
     3901 caacaaagac ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc
     3961 gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg
     4021 cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac
     4081 cctgccgatg cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg
     4141 taaaggcggt cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg
     4201 tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg
     4261 tgcaactctg ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg
     4321 tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga
     4381 ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg
     4441 cgttcagacc aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg
     4501 tcgccgtagc aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt
     4561 ttattgacct gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc
     4621 ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg
     4681 ctgtccataa tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca
     4741 aactgggtga attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga
     4801 agaagaaata aggtaggagg aagagatgga aactattgct aaacatcgcc atgctcgttc
     4861 ttctgctcag aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc
     4921 tctggatatt ttgacctaca ccaacaagaa agcggctgta caggtcaaga aagttctgga
     4981 atctgccatt gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac
     5041 gaaaattttc gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg
     5101 tgcagatcgc atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga
     5161 ctctggagac tagcaatggg tcagaaagta catcctaatg gtattcgctt gggtattgta
     5221 aaaccatgga actctacttg gtttgcgaac accaaagaat tcgctgacaa cctggacagc
     5281 gattttaaag tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc
     5341 gttatcgagc gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc
     5401 gttatcggta aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct
     5461 ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg
     5521 gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag
     5581 cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc
     5641 cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg
     5701 cacactctgc gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta
     5761 atcggcgtta aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa
     5821 caaccggaaa aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga
     5881 gcgtcgctga tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac
     5941 cgcggtctgg cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc
     6001 cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt
     6061 aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg
     6121 ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag
     6181 ccgggtaaag tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc
     6241 aagctggcag cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa
     6301 tgaaagcaaa agagctgcgt gagaagagca ttgaagagct gaacaccgag ctgctgaacc
     6361 tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc
     6421 acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg
     6481 cgggtgcgta atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat
     6541 ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggtaaatt
     6601 catcaagcgt acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga
     6661 cgtggttgaa atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg
     6721 cgttgtagag aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca
     6781 gaaatgagcc gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc
     6841 tcgatatggg gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg
     6901 agcactaaaa tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt
     6961 cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac
     7021 atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg
     7081 ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt
     7141 cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg
     7201 cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct
     7261 ctggcaccag aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga
     7321 agttatcgtg ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc
     7381 ttccggcaag gtcattgttg aaggtatcaa cctggttaag aaacatcaga agccggttcc
     7441 ggccctgaac caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt
     7501 agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg
     7561 caaaaaagtc cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg
     7621 atggcgaaac tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt
     7681 aactacaatt ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt
     7741 ggtgaagcga tcgctgacaa aaaactgctg gataacgcag cagcagacct ggcagcaatc
     7801 tccggtcaaa aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt
     7861 cagggctatc cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt
     7921 gagcgcctga tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag
     7981 tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa
     8041 atcgactacg ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg
     8101 aaatctgacg aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa
     8161 ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt
     8221 tagctgataa atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg
     8281 cttccgacga agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca
     8341 gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga
     8401 agttcgggtt gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc
     8461 tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca
     8521 tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag
     8581 ctgcggtcac catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag
     8641 aaggttttat tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc
     8701 tgaagtattt ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc
     8761 tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag
     8821 ttgtttctac ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg
     8881 gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac
     8941 cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag
     9001 gtaaaaacgg cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata
     9061 ataccctgac cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg
     9121 cgcgtgccct gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc
     9181 agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac ctgtctctgg
     9241 gtttctctca tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc
     9301 agactgaaat cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc
     9361 tgcgcgccta ccgtcgtcct gagccttaca aaggcaaggg tgttcgttac gccgacgaag
     9421 tcgtgcgtac caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc
     9481 gtatccgtcg tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg
     9541 tggtacatcg taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag
     9601 ttctggtagc tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta
     9661 acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca
     9721 tcaaagatgt atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg
     9781 cagatgctgc ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat
     9841 cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac
     9901 cgttaaaggt ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg
     9961 tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat
    10021 ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tacaacaccc
    10081 tgttaaaggt gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg
    10141 tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct
    10201 ggctaaagca tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct
    10261 ggaaaatatg aattctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat
    10321 tctggggaaa taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg
    10381 tcgtctgccg aaacacaagg caacgctgct tggcttgggt ctgcgtcgta ttggtcacac
    10441 cgtagagcgc gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt
    10501 taaagttgag gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa
    10561 ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg
    10621 tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca
    10681 gatgcctctg taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac
    10741 agccgaagtt cgtctatctg acctggctaa agtagaaggc ggtgttgtag acctgaacac
    10801 gctgaaagcg gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg
    10861 cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc
    10921 tatcgaagct gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat
    10981 tagattttca aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta
    11041 tcggtgcgct gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg
    11101 ctgtacttgc caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt
    11161 tctctggtgg tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt
    11221 cggcgtcgat cattatccag ctgctgacgg tggttcaccc aacgttggca gaaattaaga
    11281 aagaagggga gtctggtcgt cgtaagatca gccagtacac ccgttacggt actctggtgc
    11341 tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag
    11401 gcctggtgat taacccgggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag
    11461 gaacgatgtt cctgatgtgg ttgggcgaac agatcactga acgaggtatc ggcaacggta
    11521 tttcaatcat tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta
    11581 tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag
    11641 tatttgcagt gacgttcttt gttgtgtttg ttgagcgtgg tcaacgccgc attgtggtaa
    11701 actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc
    11761 tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc
    11821 cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt
    11881 cgctgtattt gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct
    11941 tcttctgttt cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga
    12001 agaagtccgg tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg
    12061 ataaagtaat gacccgcctg accttggttg gtgcgctgta tattaccttt atctgcctga
    12121 tcccggagtt catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc
    12181 ttatcgttgt tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca
    12241 gtcagtatga gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg
    12301 cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt
    12361 aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag
    12421 cataaacagc gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg
    12481 gctagattag ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa
    12541 cgggcttttc agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag
    12601 caggcattaa cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg
    12661 tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga
    12721 tcagtgagct gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg
    12781 ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt
    12841 gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca
    12901 acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata
    12961 atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg
    13021 gctcatatcc atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac
    13081 gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg
    13141 tttgcagctc aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag
    13201 aatctggaag ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg
    13261 aacgccgcag gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt
    13321 tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag
    13381 aaaatggcaa gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta
    13441 ttccttaagt ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc
    13501 cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa
    13561 aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca
    13621 gcacgtctga aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac
    13681 aacgttgtat accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc
    13741 cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca ggttagtccg
    13801 aatgacgttg taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg
    13861 gagctggctg agcagcgtga aaagccaact tggctggaag ttgatgctgg caagatggaa
    13921 ggtacgttta agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg
    13981 atcgtcgagc tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc
    14041 tgtgacagag tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc
    14101 caaggtgacc cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg
    14161 ccgtattctg ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt
    14221 actacatgag tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa
    14281 cctgaaaggg ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa
    14341 atctggcatt ggccctgtga ctgcagccga tatcacccac gacggtgatg tcgaaatcgt
    14401 caagccgcag cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat
    14461 caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga
    14521 tgagcgccca atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc
    14581 ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga
    14641 aatggaaacc aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct
    14701 ggctgaacaa ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga
    14761 agagaaacca gagttcgatc cgatcctgct gcgccctgtt gacgatctgg aattgactgt
    14821 ccgctctgct aactgcctta aagcagaagc tatccactat atcggtgatc tggtacagcg
    14881 taccgaggtt gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa
    14941 agacgtgctg gcttcccgtg gactgtctct gggcatgcgc ttggaaaact ggccaccggc
    15001 aagcatcgct gacgagtaac cggatcacag gttaaggttt tactgagaag gataaggtca
    15061 tgcgccatcg taagagtggt cgtcaactga accgcaacag cagccatcgc caggctatgt
    15121 tccgcaatat ggcaggttca ctggttcgtc atgaaatcat caagacgact ctgcctaaag
    15181 cgaaagagct gcgccgcgta gttgagccgc tgattactct tgccaagact gatagcgttg
    15241 ctaatcgtcg tctggcattc gcccgtactc gtgataacga gatcgtggca aaactgttta
    15301 acgaactggg cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt ctgaagtgtg
    15361 gcttccgtgc aggcgacaac gcgccgatgg cttacatcga gctggttgat cgttcagaga
    15421 aagcagaagc tgctgcagag taatctgaag caatgtaaaa aaacccgccc cggcgggttt
    15481 ttttataccc gtagtatccc cacttatcta caatagctgt actctttt
//
LOCUS       DAJVRL010000087        15490 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVRL010000087 DAJVRL010000000
VERSION     DAJVRL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.087"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(170..1279)
                     /gene="anmK"
                     /locus_tag="OQN02_002945"
     CDS             complement(170..1279)
                     /gene="anmK"
                     /locus_tag="OQN02_002945"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1643664.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1553..2020
                     /gene="slyB"
                     /locus_tag="OQN02_002946"
     CDS             1553..2020
                     /gene="slyB"
                     /locus_tag="OQN02_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1643665.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2067..2501)
                     /gene="slyA"
                     /locus_tag="OQN02_002947"
     CDS             complement(2067..2501)
                     /gene="slyA"
                     /locus_tag="OQN02_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1643666.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2702..2938
                     /locus_tag="OQN02_002948"
     CDS             2702..2938
                     /locus_tag="OQN02_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1643667.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2941..3798
                     /locus_tag="OQN02_002949"
     CDS             2941..3798
                     /locus_tag="OQN02_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1643668.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3798..5795
                     /locus_tag="OQN02_002950"
                     /pseudo
     CDS             3798..5795
                     /locus_tag="OQN02_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5796..6317)
                     /gene="sodC"
                     /locus_tag="OQN02_002951"
     CDS             complement(5796..6317)
                     /gene="sodC"
                     /locus_tag="OQN02_002951"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1643669.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6398..7294)
                     /locus_tag="OQN02_002952"
     CDS             complement(6398..7294)
                     /locus_tag="OQN02_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1643670.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7343..7582)
                     /locus_tag="OQN02_002953"
     CDS             complement(7343..7582)
                     /locus_tag="OQN02_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1643671.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7685..8284
                     /gene="nemR"
                     /locus_tag="OQN02_002954"
     CDS             7685..8284
                     /gene="nemR"
                     /locus_tag="OQN02_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1643672.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8321..9418
                     /gene="nemA"
                     /locus_tag="OQN02_002955"
     CDS             8321..9418
                     /gene="nemA"
                     /locus_tag="OQN02_002955"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCS1643673.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9499..9906
                     /gene="gloA"
                     /locus_tag="OQN02_002956"
     CDS             9499..9906
                     /gene="gloA"
                     /locus_tag="OQN02_002956"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1643674.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10009..10656
                     /gene="rnt"
                     /locus_tag="OQN02_002957"
     CDS             10009..10656
                     /gene="rnt"
                     /locus_tag="OQN02_002957"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1643675.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10749..15207
                     /locus_tag="OQN02_002958"
                     /pseudo
     CDS             10749..15207
                     /locus_tag="OQN02_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15251..>15490)
                     /locus_tag="OQN02_002959"
     CDS             complement(15251..>15490)
                     /locus_tag="OQN02_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1643676.1"
                     /translation="AVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGA
                     RNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3549 a         4100 c         4074 g         3767 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaag aaggaccggc aaaatgataa ttaacagttt
       61 tttcatagtc atatcccgaa gactttcctg gtccggaggg caaataccct ccttaatctt
      121 ccaagtgtaa cggcagacac gataagaaaa aattcagtta attctgatat cacgagttgg
      181 cggggaaaat agcccccagt accgtctcct ggcttgcgcc agtgacggaa ggcagatttc
      241 ctggtaatcc cgccagcgtc cgccaggcaa gccaggcgaa agccaatgct tccatgtcat
      301 cgccactaat gccaacggca tcggtggtgg tgacttctgt gcctagcagt aataccgcca
      361 gacgcgccat gagtagcgga ttacgactac ctccaccaca taccatcaat cgttcgcagc
      421 caccgctcaa caaaacttgt tcagaaatgg tcacggcggt gagttctgcc agtgtggcct
      481 gaacatcacg gggatcaaca cccggaaaat ggcgtaaatg gcgctccagc cagccgtagt
      541 taaagtattc gcgtccggtg cttttcggtg caggttgcga gaaatacggg tcgctgagca
      601 tattttgcag cagtgggaga ataactttac ccgcccgtgc ccactcggca tctttatcgt
      661 aaggtttacc tgcctgacgc cagatccagg catccatcag catgttacca ggaccggtat
      721 cgtagccgcc aaccggctgc ccaggaatga gcagtgacag attggcgatg ccgccaatat
      781 tgagcaccat tcgtcgctcg gttgggtgag ccagcaaggc atgatggaac gcaggtacca
      841 gcggtgcgcc ttgtcctccc aaggcaatat cgcgacggcg aaaatcaccg acaaccgtaa
      901 ttccggtgcg tgccacaatt tgattgttat cgccaatctg caaagtgtgt ggtgccacgc
      961 ccgtcggttc atgccagacg gtttgaccgt gacagccgat cgcaactata tctctcgcct
     1021 gcaggttttg ttccttaagc aaggcattga ccgcatcagc aaaaagtcgc ccgagttgag
     1081 tatcaagctg tccaaactgc gaaagtgtaa gctgctggcc ctggcaaata tccagtacag
     1141 cctgtttcag agacaccggg atcggccagc tcaagcttgc cagctgtgcg acccggtgtt
     1201 catcaattgt cgccaacaca acatcaacac catcaaggct ggtgcctgac ataacgccaa
     1261 taaagcggcc cgatttcata gttcatcctt tttcaatctg acgtttgcgc accactcaaa
     1321 cataaacttt tcgtgaatac catgcggaat gaccgatttt taccgttggt agtaaaacat
     1381 tatcttcaaa tcaataatca tcatgaatgt tttgtttata attggttgat cctactttca
     1441 ttatgatttg ctcatatttg gtagaacatg taaccatgga ttcacatatg ccatatactt
     1501 tgaccatgag ggatgcttgc gtggcgtttc atggtgaaca ggagattttt caatgattaa
     1561 acgcgtattg gttgtttcaa tggtaggtct gtctcttgtc ggttgtgtta ataacgacac
     1621 cctgtcaggg gatgtttata ccgcttctga agcgaaacaa gtacagaatg tcagctatgg
     1681 caccatcgtt aacgtacgtc cggtacagat tcagggcggt gatgattcca acgttatcgg
     1741 tgcaattggc ggtgctgttc ttggtggttt cctggggaat actgttggtg gcggaaccgg
     1801 gcgttctctg gctactgcag caggcgctgt tgcaggtggc gtagctggtc agggcgtaca
     1861 gagtgcaatg aacaaaacgc agggtgtcga gctggaaatt cgtaaagacg atggtaatac
     1921 catcatggtg gtacagaaac aaggcaacac tcgtttctct ccgggccaac gtgtcgtact
     1981 ggccagcaat ggcagtcagg tgaccgtttc tccgcgctaa ataagtttgc gtgtggtcag
     2041 gttactgacc acacgccccc ttcatttcac cctttggcct gtaactcaat gatattatgc
     2101 tcaagttttg cgatgagcgt aatcagttgc tccagttcct ctgcggagat gccatgtaat
     2161 atttccgcgc gggttttgtt aataacagct tccatttcgc tgatcagtgg ctctgccttt
     2221 tccgtcagtt taatacgttt agcccgacga tcgctggcac aagtttgacg cgaaattaac
     2281 cctttttctt ccagttggtc cagagtacgg accagtgatg gctgctcgat gccaatcgct
     2341 tttgccagtt gaatttgcga ctggtctgga ggtaactgat ggatattgtg taacgtaacc
     2401 caatgggttt gtgttaactc cagcggtttc aggcgatggt ctatcagagc acgccatatg
     2461 cgcaccaacc gtgccagatc agaacctagt ggcgattcca atttcatctc cttataatta
     2521 gcttgctaag atattatgcg gcttttagaa tagtgtgcag caattgtatt gctaaaacaa
     2581 atgtattgct gcatttggtt accgtcagac atatttttca gaaattgcgc gtaaattttt
     2641 cgcacttaaa gaatatttat taatctaacg caatatattc ggtcgtaaag gaatctactt
     2701 tgtgaagttt atgctcaatg caacaggatt gcccttacaa gatctggtgt tcggtgcgtc
     2761 cgtctatttt cctccgtttt tcaaagcatt cgcgtttgga ttcgtcatct ggcttgtcgt
     2821 acaccgcctg cttcgtggct ggatctacgc cggcgatatc tggcatccct tgttaatgga
     2881 tttatcgctg tttgcgattt gcgtttgcct tgctctggca atactgattg cgtggtaact
     2941 atgtcaatta aaacaattaa gtatttctca acaatcattg tagcgatagt tgcggttctt
     3001 gccggatggt ggctgtggaa ttattacatg caatctccgt ggacacgcga cgggaaaata
     3061 cgcgcagaac aggtttctat caccccacag gtgtcgggac gtattgttga gctgaatata
     3121 aaagacaacc aactggttaa cgcgggggaa cttctgctca cgatcgataa aacgcccttt
     3181 cagatcgccg agctgaacgc acaggctcag ctggcgaaag cgcaatctga cctcgcaaaa
     3241 gccaataacg aagcgaatcg tcgtcgtcat ctctcacaaa attttatttt tgccgaagaa
     3301 ctcgataccg ctaacctcaa tgttaaagcg atgcaggcca gtgttaatgc cgcacaggcg
     3361 acgctgaaac agacacaatg gcaactggcg caaacggaaa ttcgcgcccc ggtgagtgga
     3421 tgggtgacta acctcactac ccgcatcggc gattacgctg acaccgggaa accgctgttt
     3481 gcccttgtcg acagccactc gttttacgtc attggttatt ttgaagaaac caagttgcgc
     3541 catatccgcg aaggtgcacc agcacaaatt accctctata gtgataacaa aacgttacag
     3601 ggtcacgttt ccagtatcgg tcgggcgatt tatgatcaga gcgttgaaag tgactcaagc
     3661 cttattccgg atgtgaaacc taacgtcccc tgggttcgtc tcgcccaact cgttcccgtc
     3721 cgctttgcgc tggataaagt tccaggcgat gtcacgctgg tgtcgggcac cacctgtagc
     3781 attgccgtag gtcaataatg aacgcatcgt catggtcctt gcgcaatttg ccctggttca
     3841 gggccacgct ggcgcaatgg cgttatgcgt tacgcaatac cattgccatg tgtctggcgc
     3901 tgacggttgc ctattatttg aatctggatg aaccctattg ggcgatgacc tcggctgcag
     3961 tggttagctt tcccaccgtt ggcggtgtca tcagcaaaag cctcggacgc atggctggca
     4021 gtttactggg agccattgcg gcactgcttc ttgccggaca tacgctcaat gagccgtggt
     4081 tttttctatt gagcatgtcg gcgtggcttg gcttttgtac ctaggcctgt gcgcacttca
     4141 cgaataacgt cgcgtatgca tttcaactgg cgggctacac ggctgccatc atcgcctttc
     4201 cgatggttaa tattactgag gccagccagc tgtgggatat cgctcaggcg cgcgtttgcg
     4261 aggtgatagt cggtattttg tgcggcggca tgatgatgat gatcctgcct agcagttccg
     4321 atgctactgc ccttttaact gcattgaaaa acatgcacgc ccgattactg gaacatgcca
     4381 gtttactctg gcagcctgaa acaaacgatg ccattcgtgc agcacatgag ggggtgattg
     4441 ggcagatact gaccatgaat ttgctgcgta tccaggcttt ctggagccac tatcgttttc
     4501 gccagcaaaa cgcgcgcctt aatgcgctac tccaccagca attacgtatg accagtgtca
     4561 tctccagcct gcgacgtatg ttgctcaact ggccctcacc gccaggtgcc acgcgagaaa
     4621 ttctcgaaca attgctgacg gcgctcgcca gttcgcaaac agatgcttac accgtcgcac
     4681 gtattatcgc cccgctacgc ccgaccaacg tcgccgacta ccggcacgtc gccttctggc
     4741 agcgactacg ttatttttgc cgcctttatc tgcaaagtag tcaggaatta catcgtctgc
     4801 aaagcggtgt agatgatcat gccagactcg cacggacatc cgctctggct cgtcataccg
     4861 acaacgccga agctatgtgg agcgggctgc gtacattttg tacgttgatg atgattggcg
     4921 catggagtat tgcatcgcaa tgggatgccg gtgccaatgc attaacgctg gcagcaatta
     4981 gctgcgtact ctactccgcc gtcgcagcac cgtttaagtc gttgtcactg ctgatgcgca
     5041 cgctggtgtt acttttggtc aaatttggcc tgatggtcca gattagcgat ctgtggcaat
     5101 ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg cagatgccca
     5161 aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc gctgtcacta
     5221 atcccccggt gtatgatttt gctgattttc ttaactataa tctggcaaaa atcgttggcg
     5281 ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct cgtaaaagcc
     5341 gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc catccaacac
     5401 tgagtgaaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg agtaacagcc
     5461 aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg aactgttctc
     5521 atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg cgagtacggg
     5581 ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag caaaaatcac
     5641 tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt catcatcaac
     5701 ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt tcgcaacttg
     5761 agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc acaggcatag
     5821 cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc gccaacgtgg
     5881 accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc gatgacggca
     5941 tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa atgcccggta
     6001 ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc tgcggattcc
     6061 gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc atgaatatgg
     6121 aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag acctttatcg
     6181 gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt gacgaggttc
     6241 atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc cagaatggcc
     6301 agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag tgtaaaccag
     6361 tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg taccccagtg
     6421 cagctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct gcttcgacag
     6481 ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc gacggtaaac
     6541 gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc tccgccacca
     6601 cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga ccaccaccaa
     6661 ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc gtgccatcca
     6721 gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg gtaaacggca
     6781 gacgtgattg caacagggca aattgcgcag gcgtaaagtt cgatacgcca aaatgacgca
     6841 ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca tccattaacg
     6901 ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta attagcgact
     6961 gttcggcgct cttaatgatg tgattgcggt cagtgatgta atgaccaatg acgttttctt
     7021 cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca cgcaggtgag
     7081 gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca taaatatcag
     7141 catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg accagctggc
     7201 gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg gaaaactccg
     7261 ggccttccgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat cagatataag
     7321 aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg tctgatgacg
     7381 ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc agcacctctt
     7441 gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta cgaaaacagc
     7501 cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc tggacgggaa
     7561 agaactctaa ttgctccgcc acctcgccct cctcagataa gattattacc attattgaag
     7621 ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta ctacactcca
     7681 acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcttggcga cgggcgagca
     7741 actttgcctg caacgtggat tcaccgggat ggggctaagc gaattactaa aaaccgctga
     7801 agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg gcgttgccat
     7861 gcttgagcgt cattacgccg catatcacca gcgactggct gagttgctgc aatccggcga
     7921 aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc agttttgcca
     7981 acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt gcgatctgtc
     8041 agaagatatg cgcagcgcga tggataaagg tgctcgcggc gtgatcgccc tgctctcgca
     8101 ggctctggaa aatggccgtg agaaccattg tttaaccttt tgtggcgaac cgctacaaca
     8161 ggcacaagtg ctttacgcac tatggcttgg cgcgaatctg caggccaaaa tttcgcgcag
     8221 tttcgagcca ctggaaaacg cgctggccca tgtaaaaaac attattgcga cgcctgccgt
     8281 ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg aaaaactgta
     8341 ttccccactg aaagtgggcg cgatcacggc ggcaaaccgt atttttatgg caccgctgac
     8401 gcgtctgcgc agtattgaac cgggtgacat tcctaccccg ttgatggcgg aatactatcg
     8461 ccaacgtgcc agtgccggtt tgattattag tgaagccaca caaatttctg cccaggcaaa
     8521 aggatatgca ggtgcgcctg gcatccatag tccggagcaa attgccgcat ggaaaaaaat
     8581 caccgctggc gttcatgctg aaaatggtca tatggccttg cagctgtggc acaccggacg
     8641 catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt cagcacttag
     8701 cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgcg ttgaaacatc
     8761 catgccgcgt gcgcttgaac tggaagagat tccgggtatc gtcaatgatt tccgtcaggc
     8821 cattgctaac gcgcgtgaag ccgcttttga tctggtagag ctccactctg ctcacggtta
     8881 tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt acggcggcag
     8941 cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg aagaatgggg
     9001 tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca cagataacgg
     9061 cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac gcggcattgc
     9121 ttatctgcat atgtcagaac cagattgggc ggggggtgaa ccgtatactg atgcgttccg
     9181 cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat acacagtaga
     9241 aaaagctgaa acgctgatcg gcaaagggtt aattgatgcg gtggcatttg gtcgtgactg
     9301 gattgcgaac ccggatctgg tcgcccgctt gcagctcaaa gctgagctta acccacagcg
     9361 tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga cgttgtaatc
     9421 caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga atctgttagc
     9481 cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg cgatttgcaa
     9541 cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac cagcgaaaac
     9601 ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga agaagcggtg
     9661 attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc ttatggtcac
     9721 atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca aaacgggggt
     9781 aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc gtttgtggaa
     9841 gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg cggtctgggc
     9901 aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca tcgacaagta
     9961 atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat gtccgataac
    10021 gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt gatcgatgtt
    10081 gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc catcaccctg
    10141 aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca cgtcgaacca
    10201 tttgtcggcg caaatttgca accagaagcc ctcgccttca acggcattga cccgaacgat
    10261 cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa agttgtacgt
    10321 aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa cgccaatttt
    10381 gatcacagct ttatgatggc cgccgcagaa cgcgcctcgc tgaaacgtaa cccgttccac
    10441 cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca aaccgtattg
    10501 tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca ctccgcgctg
    10561 tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg gaaacgtctg
    10621 ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct gatgacatgc
    10681 aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat tcacggggaa
    10741 tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg ttttcaccgg
    10801 cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag tcgcaaacct
    10861 ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc tccgggaaaa
    10921 cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc gaagataccc
    10981 gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata aaagccctgg
    11041 gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat gaacggcggc
    11101 ggcgcggcga aacggtaatt attgatgaag tccacgcggt agcgggcagt aaacgtggtg
    11161 cgcatctggc gttaagtctg gagcggctcg atgcgctgct ccacacctca gcacagcgaa
    11221 ttggcctttc tgccactgtg cgctcagcca gcgatgtggc agcatttctt ggtggcgatc
    11281 gcccggttac ggtagtcaac ccgcccgcaa tgcgccatcc gcagatacga attgtcgtac
    11341 cggtcgccaa tatggatgat gtctcatcgg tcgccagcgg caccggcgaa gacagccatg
    11401 ccggccggga aggctccatc tggccatata ttgaaacggg tatccttgat gaagtgttgc
    11461 gccatcgctc gaccattgtc tttactaatt cacgcgggct ggcggaaaaa ctgacagcac
    11521 gattaaatga gctttacgcc gcacgcttac agcgttcccc gtctatcgcc gttgatgcgg
    11581 cccatttcga gtcgacctcc ggcgcaacct ctaaccgtgt acaaagtagc gacgttttta
    11641 ttgcccgctc acaccacggc tccgtctcta aagaacaacg agcaatcacc gaacaggcgc
    11701 tgaaatcggg tgaattacgc tgcgtggtcg caacctccag tcttgaactg gggattgata
    11761 tgggcgcggt ggatctggtg attcaggtgg caacgccgct ttctgttgcc agtgggttac
    11821 aacgcattgg tcgcgccgga catcaggttg gcggtgtatc taaagggctg tttttccccc
    11881 gtacccggcg tgatttagtc gattccgcag tcattgtaga gtgtatgttc gcaggcaggc
    11941 tggaaaacct gacaccaccg cataatcctc tcgacgtcct tgcgcagcaa accgttgccg
    12001 ccgcggcgat ggatgcatta caggtagacg aatggtactc ccgcgtacgc cgtaccgcac
    12061 cgtggaaaga tctgccaaga cgtgtttttg acgccacgct ggatatgctt tccgggcgct
    12121 atccctctgg cgatttttct gcttttcgcc ccaaactggt ctggaacagg gagaccggga
    12181 tattgaccgc ccgacctggc gctcaattgt tggcggttac cagcggcggc accattccgg
    12241 atcgtggcat gtatagcgtg ttattacccg aaggtgaaga aaaggccggt tcgcggcggg
    12301 tgggtgaact ggatgaggag atggtatatg agtcgcgggt gaacgacatt atcactctcg
    12361 gcgctacctc atggcggatc cagcaaatca cccgcgatca ggtgattgtg actcctgctc
    12421 cgggtcgttc tgcccagctc cccttctggc gtggtgaagg taacggacgt ccggctgaat
    12481 taggcgagat gatcggcgat tttcttcatt tactggcgga tggcgcgttc ttttccggga
    12541 ctattccccc gtggctggca gaagaaaata cgaacgccaa tattcagggg ttgattgacg
    12601 agcaacgcaa cgcgacgggc atcgttccgg ggagtcgcca tctggtcctc gaacggtgcc
    12661 gtgatgaaat tggcgactgg cgtattattt tgcactctcc ctatggaaga cgggtgcatg
    12721 aaccctgggc gctggcgatt gccgggcgaa tacatgccct atggggcgct gacgcgtctg
    12781 tggtcgccag tgatgacggc attgttgcgc gcattcctga cactgatggt aaattgcccg
    12841 acgccgcgat ttttttgttt gaaccagaaa agttgctgca aattgtccgc gaggcggtag
    12901 gcagctcggc acttttcgcc gcccgttttc gcgaatgcgc cgcgcgggca ttattaatgc
    12961 cgggccgcac gccgggccat cgcaccccgc tttggcaaca acggctgcgc gccagtcagt
    13021 tgctggaaat cgctcaggga tatccggatt ttccggtcat tctcgaaacc ctacgcgaat
    13081 gtctgcaaga tgtttatgat cttcccgcac tggaacgttt gatgcgtcgc ctgaacggtg
    13141 gcgaaattca aatatccgat gtaacgacca ctacgccctc gcctttcgcc acaagtttat
    13201 tgttcggcta tgtcgcggaa tttatgtacc agagcgacgc cccgctggca gagcgccggg
    13261 catccgtact gtcgctggac agcgagttac tgcgcaatct actcggacag gtcgatccgg
    13321 gggaattact cgacccgcag gtcattcgcc aggtggaaga agagttgcaa cgactggctc
    13381 ctggtagaag agcgaaaggt gaagaaggat tgttcgacct gctgcgcgaa ctggggccaa
    13441 tgaccgttga agacctggcg caacggcata caggcagcag tgaagaggtt gcgtcgtatc
    13501 tggaaaatct tcttgcagta aaacgaatct tcccagcgat gattagcgga caggagcgtc
    13561 ttgcctgtat ggatgatgcc gccaggctgc gtgatgccct cggcgtacgg ctaccagagt
    13621 cattgccaga gatttattta catagagtca gttacccgct tcgcgacctc tttctgcgct
    13681 atctccgggc tcatgctctg gtcacggctg aacaactggc tcatgagttt agtctcggta
    13741 ttgccattgt cgaagagcag cttcagcaac tgcgtgaaca gggtctggtg atgaatctgc
    13801 aacaaaacat ctgggtgagc gatgaagtat ttcgtcgtct gcgtttgcgc tcgctgcaag
    13861 ccgccagaga agcgacgcgt cccgttgcag ccacgaccta tgcgcgattg ctgctggaac
    13921 gtcagggcgt attacccgcc accgatggta gcccggcgct ctttgcctca acatctccag
    13981 gcgtttatga gggcgtagat ggcgtgatgc gggtgatcga acagcttgcc ggagtcggtt
    14041 tacccgcctc actctgggaa agccagatcc tgcctgcccg cgtacgcgac tattcgccag
    14101 aaatgctcga tgaattactg gcaaccggag cggttatctg gtcgggacaa aaaaaagctg
    14161 ggtgaagatg acggcctggt ggcactgcat ctacagaaat atgccgcaga atcgttcact
    14221 cccgccgaag cggatcaggc gaatcgttcg gcgctgcagc aagcgataat cgctgttctg
    14281 gctgacggag gagcctggtt tgcacaacaa atcagccaac ggatacgcga taaaatcgtc
    14341 gaatcggttg attcctcggc actgcaagag gcgttatggg cgctggtctg gcaaggcgtc
    14401 attaccagcg atatttgggc accgttacgc gccctcaccc gcagcagttc caacgcacgc
    14461 acctcaactc gccgcagtca ccgggctcgt cgtgggcgtc ctgtctatgc acaacccgtc
    14521 tcgccgcggg tatcttacaa cacaccaaat ctggctggac gctggtcgtt attgcaggtg
    14581 gagccactaa acgataccga aaggatgctg gcgctggcgg aaaatatgct cgaccgctac
    14641 ggcatcatca gtcgtcaggc ggtgatagcc gaaaatatcc ctggcgggtt tccatcgatg
    14701 caaacacttt gtcgaagtat ggaagactcc gggcgaatta tgcgaggtcg ttttgtagaa
    14761 ggtctgggtg gcgcgcaatt cgctgaacgt ctgactattg accgattgcg cgatctggcg
    14821 acacaagcca cgcaaacacg ccactataca ccagtggcgc tctccgccaa cgatccggct
    14881 aatgtgtggg gaaatcttct gccctggcct gcacatccgg caacgctggt accaacgcgt
    14941 cgggcgggtg cgctggtggt cgtttctggc ggcaaattgt tactctatct ggcgcaaggc
    15001 ggcaaaaaaa tgctggtctg gcaggaaaag gaggaattac tcgccccaga gattttccag
    15061 gcgctgacca ccgcactgcg tcgcgaacca aggctgcgct ttacgctaac agaagtgaat
    15121 gatcttccgg tccggcaaac gccgatgttt acgctgctgc gtgaggcggg attttcaagt
    15181 tcgccacaag ggctggattg gggatagttg acatcctcca cgccctgaaa gacgtggttc
    15241 cctgctacgt tcaagctgtc gcctgaatca tttcggtggg ttcctgcttc aacgagcggc
    15301 ctgactgcac catctctcca caggcaagaa cggcgtgccc cgccgctaaa atgttacgag
    15361 cgccatttac atcggcgttc gctgtatatc cacatacctg gcatctgaat ttactttgtg
    15421 acaggcggtt ttctttcgct gtatgaccac agcacgcgca acgctggctt gtgtacgctg
    15481 gcggaacagc
//
LOCUS       DAJVRL010000088        15410 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVRL010000088 DAJVRL010000000
VERSION     DAJVRL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.088"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..1175)
                     /locus_tag="OQN02_002960"
     CDS             complement(<1..1175)
                     /locus_tag="OQN02_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1643677.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="OQN02_002961"
     tRNA            complement(1374..1458)
                     /locus_tag="OQN02_002961"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="OQN02_002962"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="OQN02_002962"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1643678.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="OQN02_002963"
     CDS             2802..4304
                     /locus_tag="OQN02_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1643679.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OQN02_002964"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OQN02_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1643680.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OQN02_002965"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OQN02_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1643681.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="OQN02_002966"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="OQN02_002966"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1643682.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="OQN02_002967"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="OQN02_002967"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1643683.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="OQN02_002968"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="OQN02_002968"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1643684.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="OQN02_002969"
     CDS             complement(11869..13065)
                     /locus_tag="OQN02_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCS1643685.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="OQN02_002970"
     CDS             13258..13761
                     /locus_tag="OQN02_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1643686.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OQN02_002971"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OQN02_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1643687.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="OQN02_002972"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="OQN02_002972"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1643688.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3976 a         3931 c         3966 g         3537 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcgtc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatca ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgtttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc tgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaaa gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca tacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 agcgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DAJVRL010000089        15102 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVRL010000089 DAJVRL010000000
VERSION     DAJVRL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.089"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OQN02_002973"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OQN02_002973"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1643689.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OQN02_002974"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OQN02_002974"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1643690.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OQN02_002975"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OQN02_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCS1643691.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OQN02_002976"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OQN02_002976"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1643692.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OQN02_002977"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OQN02_002977"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1643693.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OQN02_002978"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OQN02_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1643694.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OQN02_002979"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OQN02_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCS1643695.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OQN02_002980"
     CDS             complement(8065..9207)
                     /locus_tag="OQN02_002980"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCS1643696.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OQN02_002981"
     CDS             complement(9216..10229)
                     /locus_tag="OQN02_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCS1643697.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OQN02_002982"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OQN02_002982"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1643698.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OQN02_002983"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OQN02_002983"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1643699.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OQN02_002984"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OQN02_002984"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1643700.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OQN02_002985"
     CDS             complement(12827..13951)
                     /locus_tag="OQN02_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1643701.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OQN02_002986"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OQN02_002986"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1643702.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4294 a         3399 c         3107 g         4302 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg gaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgc cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgctaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaacatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg ca
//
LOCUS       DAJVRL010000090        15050 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVRL010000090 DAJVRL010000000
VERSION     DAJVRL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.090"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..176)
                     /locus_tag="OQN02_002987"
     CDS             complement(<1..176)
                     /locus_tag="OQN02_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643703.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHG"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="OQN02_002988"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="OQN02_002988"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1643704.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="OQN02_002989"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="OQN02_002989"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1643705.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="OQN02_002990"
     CDS             complement(2012..2884)
                     /locus_tag="OQN02_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS1643706.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="OQN02_002991"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="OQN02_002991"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1643707.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="OQN02_002992"
     CDS             complement(3576..4361)
                     /locus_tag="OQN02_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1643708.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="OQN02_002993"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="OQN02_002993"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCS1643709.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5459..6355
                     /gene="yihU"
                     /locus_tag="OQN02_002994"
     CDS             5459..6355
                     /gene="yihU"
                     /locus_tag="OQN02_002994"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS1643710.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6379..7257
                     /gene="yihT"
                     /locus_tag="OQN02_002995"
     CDS             6379..7257
                     /gene="yihT"
                     /locus_tag="OQN02_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCS1643711.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7274..8515
                     /gene="yihS"
                     /locus_tag="OQN02_002996"
     CDS             7274..8515
                     /gene="yihS"
                     /locus_tag="OQN02_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCS1643712.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8653..9555
                     /locus_tag="OQN02_002997"
                     /pseudo
     CDS             8653..9555
                     /locus_tag="OQN02_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9754..11790
                     /gene="yihQ"
                     /locus_tag="OQN02_002998"
     CDS             9754..11790
                     /gene="yihQ"
                     /locus_tag="OQN02_002998"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCS1643713.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11836..13210
                     /locus_tag="OQN02_002999"
                     /pseudo
     CDS             11836..13210
                     /locus_tag="OQN02_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13253..14656
                     /locus_tag="OQN02_003000"
     CDS             13253..14656
                     /locus_tag="OQN02_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1643714.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14724..>15050
                     /gene="ompL"
                     /locus_tag="OQN02_003001"
     CDS             14724..>15050
                     /gene="ompL"
                     /locus_tag="OQN02_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCS1643715.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3699 a         3874 c         3744 g         3733 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatt tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcatcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 agcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aaccgataaa ttgattgccg atatgcaagc caaaggcttc agcatgatgg atgttccggt
     5821 aggccgtact tctgcaaatg ccattaccgg tactctgtta ctgctggctg gcggcaccgc
     5881 tgaacaagtt gaacgtgcca caccgatcct gatggcgatg ggcagtgagt tgatcaacgc
     5941 tggcggtccg ggcatgggga tccgcgttaa gctcatcaac aactacatga gcatcgcgct
     6001 caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc ccttcgatgt
     6061 tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggt cacttcacca cttcctggcc
     6121 aaacaaagtc ctcagcggag atctttctcc cgccttcatg atcgatcttg cccataagga
     6181 tcttggcatc gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc tgggggccgc
     6241 ctcacgggag gtttatagcc aggcgcgcgc agcgggtcac ggtcgccagg actggtccgc
     6301 cattctggaa caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact
     6361 ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg cgcatcgggc
     6421 ggttttgcca tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct
     6481 ggggcacccg ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag
     6541 gccctctcgc cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg
     6601 gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt
     6661 cctggcaacg gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag
     6721 atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat
     6781 gcgcagcaac gtctggatat ggtgaaagag ttcaacgaac tgtgccactc acacggtctg
     6841 gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa
     6901 caagcgatca tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt
     6961 gaaatgccgc tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg
     7021 aatgaccata tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc
     7081 ccgcgtgccg tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca
     7141 gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca
     7201 ccgaaattac aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat
     7261 gaggataaaa ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga
     7321 aaccgaccgc atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt
     7381 aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat
     7441 gttgcacgtt tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga
     7501 tcacggtatt aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc
     7561 ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc
     7621 tctgctgggt gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga
     7681 ttacaccatt gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga
     7741 gtcctgggac gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca
     7801 cgcggtggaa gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg
     7861 cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt
     7921 taacgaacac ttcgataccc agtggaatcc gctgccggat tacaacaaag ataacccggc
     7981 gcatcgcttc cgcgcgttcg gaggtacacc aggccactgg atcgaatggg gccgtttaat
     8041 gctgcacatc catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga
     8101 agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggagcgga
     8161 cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg
     8221 gcctatcgtc gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca
     8281 gtatgaaacc tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga
     8341 aaatggttcc tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga
     8401 cggcaaacag gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc
     8461 gcccggcatg gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat
     8521 ctgaaaatag ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga
     8581 ccgtccggtg cagtttttgg gtttgacatc agcctcacgg gaggctgtat gtcgttaatt
     8641 aaggtgcctt gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc
     8701 gcgggggatt atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt
     8761 cagggttgtc atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt
     8821 aaagtgctta ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag
     8881 gaatatcagt tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc
     8941 tggcgagact ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg
     9001 ccgcccagtt acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca
     9061 cacacgggtc tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat
     9121 ggcgtgggta ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc
     9181 cagcttccgg caaactaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac
     9241 cacgttgatg agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc
     9301 gaccacacct ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag
     9361 gcgctttcgg taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa
     9421 ttacagcgtc agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca
     9481 ggtatcgact tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac
     9541 ggacagagaa aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg
     9601 ctaaaggcaa cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa
     9661 gtttttatat atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca
     9721 acaatctcgt tctcaactct atggagatta gttatggata cgccacgtcc acagttatta
     9781 gattttcaat ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt
     9841 ttaacccata acaaagataa tccttgttta tggattggct caggtatagc ggatatcgat
     9901 atgttccgcg gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac
     9961 gccatcgtca gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt
    10021 agcgccacgc tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac
    10081 gacaacctta accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc
    10141 tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg
    10201 accagtgaac aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc
    10261 aaagaaaatg cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc
    10321 acgcagaagt attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc
    10381 ccggaatacc atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct
    10441 gacacataca tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg
    10501 cccgactgga tttatgacgg ggtaacgctc ggcattcagg gcgggacgga agtgtgccag
    10561 aagaaactgg acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac
    10621 tggtctggga tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac
    10681 agcgaaaact acccgcaact ggattcacgc attaagcagt ggtatcagga gggcgtgcag
    10741 ttcctggcct atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca
    10801 caacacggct atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag
    10861 ttttacggcg gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg
    10921 atcaaaaaga acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat
    10981 ctgcccaccg acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctgg
    11041 cctgcgctgt gggcgaagtg taactacgaa gcccttgaag aaacgggcaa gctcggcgag
    11101 atcctcttct ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg
    11161 gcgggcgacc agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg
    11221 gcgctgtcgc tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc
    11281 accctgtttg agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc
    11341 ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac
    11401 ggcgatgcgg aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa
    11461 ccttatctga aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg
    11521 ctgttcctgc attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta
    11581 ggccgcgaca ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat
    11641 ctgccggagg ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt
    11701 accgttaatg cgcccatcgg caagccgccg gtcttttatc gcgccgatag cgaatgggcg
    11761 gcactgttcg cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc
    11821 aaagggagaa catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc
    11881 tttaaagaga aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc
    11941 ggtacgcttt atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc
    12001 ggcattatct ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt
    12061 atgttggatt cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat
    12121 gcgtcattcc cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc
    12181 accggtaaaa cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg
    12241 atgaactgct cctacggcgc aatggtcccg gcaatcacca aaaaccccaa cgaacgcgcc
    12301 tcactggcgg catggcgtca gggcggcgct acgctgggcc tgctgctgtg cacggtggga
    12361 ttcgtgccag ttatgaatct tatcgaaggt aatcagaaac ttggctatat cttcgccgcc
    12421 acgctgtttt cactgttcgg cctgctgttt atgtggatct gctactcagg cgtgaaagag
    12481 cgttatgtcg aaacccaacc aaccaatccg gcgcaaaagc ctggcctgtt gcaatctttc
    12541 cgcgcaattg ccggtaaccg cccactgttc attctgtgta ttgccaacct ctgcacctta
    12601 ggggcgttta acgtcaagct cgccattcag gtctattaca cccagtacgt acttaacgat
    12661 cccatcctgt tgtcgtatat gggatttttc agcatgggct gtattttcat cggcgtgttc
    12721 ctgatgcctg gcgcagtcag acgttttggt aagaagaagg tctatatcgg cggcctgctg
    12781 atttgggtgc tgggcgatct gctcaactat ttcttcggcg gcggctcggt cagctttgtg
    12841 gcgttctcct gcctggcgtt cttcggctca gcgcctgaac tgggcgctgg tttccgacac
    12901 cgtcgagtac ggcgagtggc gcaccggcgt gcgttcggaa ggcacggtct acaccggctt
    12961 caccttcttt cgcaaagtgt ctcaggcgct ggctggtttc ttccccggct ggatgctgac
    13021 gcaaattggc tatgtgccga acgtcgcaca ggctgaccac accattgaag ggttacgcca
    13081 gttgatcttc atctacccaa gcgcactggc ggtagtcacc attgtggcga tgggttgctt
    13141 ctacagcctg aacgagaaga tgtatgtccg cattgtggaa gagatagaag cccgtaaacg
    13201 cacggcgtaa taataataaa cgacgccctg cggggcgtta taaggagtga ttatgtctga
    13261 ccataatcca ctgacattaa aactgaatct gcgggaaaaa atcgcctatg gtatgggcga
    13321 cgtcggttcg aatttaatgc tctgcatcgg tactctgtat ctcctcaaac tttataccga
    13381 tgaattaggg atgcctgctt actatggcgg gattatcttt ctggtcgcga agttttttac
    13441 cgcgtttacc gatatgctca ccggattttt actcgactcg cggaaaaata ttgggcctaa
    13501 aggcaaattt cgtcccttta ttttatatgc agcagttccg gcggcgttaa ttgcaacgct
    13561 ccagtttatc gccaccacct tttgtttgcc ggttaaaacg acgattgcca ccacgctgtt
    13621 tatgatgttt ggactttcat acagtctgat gaactgctcg tatggtgcga tgatcccggc
    13681 aattaccaaa aacccgaatg agcgcgcgca gctcgcggct taccgccagg gtggtgcaac
    13741 catagggcta ttgatttgta ccgtggcgtt tattccgttg cagtcgcttt tttctgactc
    13801 aaccgtcggt tatgcctgtg cggcacttat gttctccatt ggcggcttta tttttatgat
    13861 gctgtgctac agaggcgtca aagagcatta tgtggacaca gcgccaaccg gacataaagc
    13921 cagtattctc aaatcttttt gcgcgatatt tcgtaatcca ccattgctgg ttttatgcat
    13981 tgctaacctg tgtactctgg cggcatttaa tatcaaactg gcgattcagg tctattacac
    14041 ccagtatgtg ctgaatgata ttaatttatt gtcgtggatg ggattcttca gtatgggatg
    14101 catcctcgtc ggcgtattac tggtgccagt aactgtaaaa tgttttggta aaaaacaggt
    14161 ttatttagct ggcatggtgc tatgggcggt gggtgatata ctgaattatt tctggggaag
    14221 taactctttc actttcgtca tgttctcttg tgtcgccttt tttggcacgg cctttgtaaa
    14281 cagcctgaac tgggcactgg taccagatac cgtagattac ggtgaatgga aaaccggtat
    14341 tcgtgccgaa ggttctgttt ataccggtta taccttcttt cgtaaaattt ctgccgcact
    14401 tgctggcttc ttgccaggca ttatgctgac gcaaattggt tatgttccca acatcgcgca
    14461 aagcgatgcg acattacagg gtttgcgtca gctcattttt atctggcctt gtgcactggc
    14521 aattattgct gcattgacga tgggattctt ttacacactc aatgaaaaac ggtttgcatt
    14581 gattattgag gaaatcaacc aacgcaaaaa taaagagatg gcgacagaaa aaaaaacggc
    14641 ttccgtaacg ttataaatgt caatagccgc tatttccatc ctggtgggtg gcggcctccc
    14701 tacgtttaaa aaatggactt attatgaaaa agattaatgc aataatttta ttatcttctc
    14761 tgacctctgc ctcggtattt gctggcgcat acgtcgaaaa cagggaggca tataatcttg
    14821 cctccgatca gggcgaggtt atgctacgtg tgggttataa cttcgatatg ggcgcgggta
    14881 ttatgttaac caatacctac acctttcagc gagaagatga actaaaacat ggctataatg
    14941 aaattgaagg ttggtatcct ttattcaaac caaccgataa attaaccatc cagcccggtg
    15001 gcttaattaa tgataagagt atcagttcgg gtggtgcagt gtatctggac
//
LOCUS       DAJVRL010000091        15028 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVRL010000091 DAJVRL010000000
VERSION     DAJVRL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.091"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..236
                     /locus_tag="OQN02_003002"
     CDS             <1..236
                     /locus_tag="OQN02_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCS1643716.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="OQN02_003003"
     CDS             246..884
                     /locus_tag="OQN02_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1643717.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="OQN02_003004"
     CDS             887..2050
                     /locus_tag="OQN02_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1643718.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3759
                     /locus_tag="OQN02_003005"
     CDS             2134..3759
                     /locus_tag="OQN02_003005"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1643719.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="OQN02_003006"
     CDS             complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="OQN02_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1643720.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="OQN02_003007"
     CDS             complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="OQN02_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1643721.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4811..5560
                     /gene="yjfP"
                     /locus_tag="OQN02_003008"
     CDS             4811..5560
                     /gene="yjfP"
                     /locus_tag="OQN02_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1643722.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="OQN02_003009"
     CDS             complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="OQN02_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1643723.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="OQN02_003010"
     CDS             complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="OQN02_003010"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1643724.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7839..9236
                     /gene="ulaA"
                     /locus_tag="OQN02_003011"
     CDS             7839..9236
                     /gene="ulaA"
                     /locus_tag="OQN02_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCS1643725.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9252..9557
                     /gene="ulaB"
                     /locus_tag="OQN02_003012"
     CDS             9252..9557
                     /gene="ulaB"
                     /locus_tag="OQN02_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1643726.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9567..10031
                     /gene="ulaC"
                     /locus_tag="OQN02_003013"
     CDS             9567..10031
                     /gene="ulaC"
                     /locus_tag="OQN02_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1643727.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10045..10695
                     /gene="ulaD"
                     /locus_tag="OQN02_003014"
     CDS             10045..10695
                     /gene="ulaD"
                     /locus_tag="OQN02_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1643728.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10705..11541
                     /gene="ulaE"
                     /locus_tag="OQN02_003015"
     CDS             10705..11541
                     /gene="ulaE"
                     /locus_tag="OQN02_003015"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1643729.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11541..12227
                     /gene="ulaF"
                     /locus_tag="OQN02_003016"
     CDS             11541..12227
                     /gene="ulaF"
                     /locus_tag="OQN02_003016"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1643730.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12356..12631)
                     /locus_tag="OQN02_003017"
     CDS             complement(12356..12631)
                     /locus_tag="OQN02_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1643731.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12958..13353
                     /gene="rpsF"
                     /locus_tag="OQN02_003018"
     CDS             12958..13353
                     /gene="rpsF"
                     /locus_tag="OQN02_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1643732.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13360..13674
                     /gene="priB"
                     /locus_tag="OQN02_003019"
     CDS             13360..13674
                     /gene="priB"
                     /locus_tag="OQN02_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1643733.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13679..13906
                     /gene="rpsR"
                     /locus_tag="OQN02_003020"
     CDS             13679..13906
                     /gene="rpsR"
                     /locus_tag="OQN02_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1643734.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13948..14397
                     /gene="rplI"
                     /locus_tag="OQN02_003021"
     CDS             13948..14397
                     /gene="rplI"
                     /locus_tag="OQN02_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1643735.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14468..>15028)
                     /locus_tag="OQN02_003022"
     CDS             complement(14468..>15028)
                     /locus_tag="OQN02_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1643736.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3510 a         3666 c         4050 g         3802 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cattttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaagccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcgattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatgcgca ggttgaggct
     2521 gggtcgttat gtccaataac gatgaccttt gccgccacgc cattgttgtt acagatgtta
     2581 cccacgccgt ttcaggactg gaccacgccg ctgctgagcg atcgctacga ttctcactta
     2641 ttgccaggtg ggcaaaaacg cggtttgttg attggcatgg gaatgacgga aaagcagggc
     2701 ggttccgatg tcatgagcaa caccacccgt gcagagcgtt tggaagatgg ctcttatcgg
     2761 ctggtggggc ataaatggtt tttctcggtg ccgcaaagcg atgcgcatct ggtgctggcg
     2821 cagaccgcgg gcggtctgtc ctgctttttt gtgccgcgct ttttgcctga cgggcaacgc
     2881 aacgcgattc gcctcgagcg gctgaaagat aagctgggta atcgctctaa cgccagttgc
     2941 gaagtggagt ttcaggatgc cattggctgg ttattggggc aggaagggga aggaattcgt
     3001 ctgatcctga aaatgggggg gatgacgcgt tttgattgcg ccctgggtag ccatgccatg
     3061 atgcgccgtg cattttcgct ggcgatttat catgcacatc aacgccatgt ttttggtaat
     3121 ccattgatcc aacagcccct tatgcgtcat gtcttaagtc gcatggcact tcagcttgaa
     3181 gggcaaacgg cgttgctgtt tcgtcttgcg cgagcgtggg accggcgtgc cgatgccaaa
     3241 gaagccctgt gggcgcgttt atttacgcct gcggcaaaat ttgtgatctg caaacgaggt
     3301 atgccgtttg tggccgaagc gatggaggtg ctgggcggca ttggttattg cgaggagagc
     3361 gagctgccgc ggctttaccg ggagatgccg gtaaacagta tttgggaagg ttccggcaat
     3421 attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata cgacttattg
     3481 tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc ggttcgtcgt
     3541 ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac tcatcagcta
     3601 ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat ggcgcaggcg
     3661 tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca gatccagaat
     3721 gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac tgatgcgacg
     3781 ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag gcgctcgcgc
     3841 cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat cgcctgtacg
     3901 cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca tgacgcacct
     3961 tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc tgaaatattg
     4021 ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact ggcgaattct
     4081 gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg tgaggcaagt
     4141 aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc attccatcgc
     4201 ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa cgcataggga
     4261 cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt acaaaatggc
     4321 ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa cgtaataatc
     4381 agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg gagaaccccg
     4441 aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc ggcgaatctc
     4501 ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg cgctacaggc
     4561 acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat tacgtttcct
     4621 gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc gaattgttac
     4681 ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct caacattatg
     4741 aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac cagacaaaaa
     4801 ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg ttcttcatgc
     4861 ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc acggctttac
     4921 ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg gtttgcgggt
     4981 gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag cgcggcggtt
     5041 aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt tacgtgcggc
     5101 aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg cgtcgatggg
     5161 cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta ccgccagcat
     5221 gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc tgatacctga
     5281 aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag agtgggaagc
     5341 gacaaaccac ctggaacaac ttggggacag acctctactg ctgtggcatg gcctcgacga
     5401 tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg agacgggacg
     5461 ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgtatta cgcctgaggc
     5521 gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa tgctgacccc
     5581 ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga tcagcatatc
     5641 gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac tgctatcaac
     5701 cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg ctgtcagcat
     5761 atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca tccagtgccc
     5821 ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac ttttgttgta
     5881 ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat ttgccagcgg
     5941 tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa gcagaaacgc
     6001 ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg aggccgcttt
     6061 agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg gcgtccagcg
     6121 cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt tgccgctttc
     6181 gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac gctcaacgac
     6241 tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt gatgtctttg
     6301 tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat ttgaaatagc
     6361 caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata aacgacagat
     6421 tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc gaaaccgcgc
     6481 ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc gccaacctgc
     6541 cagataaacg gcttgaagcc atacttcaga cgatcttttt tgatctccca cagcacgcgg
     6601 atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg gataaccact
     6661 ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt gtcggtaata
     6721 ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc gttaccgtgc
     6781 ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc gccaggggtt
     6841 ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc agccgctttt
     6901 tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc atgaatttca
     6961 atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc tttcggtacg
     7021 ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac gtcatcagca
     7081 cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg atcgtgagtc
     7141 gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg ggtggtgcgt
     7201 aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc ctgtttcatt
     7261 aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca aacgttggtg
     7321 ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc aaatgtgcca
     7381 ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc cgggaaagtg
     7441 ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat ttaccttcat
     7501 gatagttcaa ttcgaatcaa catgtgattg gttttgatta atcctgacac tattccttca
     7561 ggaaggcaat gaccattttt tgactttttg ccagggaagt tgttgttgat tttgagtgtg
     7621 gaaagattta atggaatgtg tagttaaatt aactaaatga atttaaataa ataattgttt
     7681 cgttgtgtga atcacactct atccgtgtgg aattatttgc gggtcgcgcc acatttaatc
     7741 ataaataatc ttgttgtgat tacttttgaa aattagagtg agtacacaac attccgggtg
     7801 tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc tacaacatct
     7861 ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt attgtgacct
     7921 gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc acgattaaaa
     7981 ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc accttcaaac
     8041 cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc gatacttacg
     8101 cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta ggttacgcgg
     8161 tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt accggtattc
     8221 gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt gccgttacgt
     8281 tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt ctggtttcgc
     8341 tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg acggacggct
     8401 gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat aaagttgcgc
     8461 cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc tggctgaata
     8521 ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt ggtgccattc
     8581 tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac tggacggtct
     8641 atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact cagggtgtgc
     8701 gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc ctgatccctg
     8761 gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac gccgtggtct
     8821 ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg gtcgcctgcg
     8881 gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac gccaccatcg
     8941 gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt ctggtgatgg
     9001 ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg agtgcctgga
     9061 tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc ttctccatcg
     9121 gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc ttcgctggcc
     9181 gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct gcttaataag
     9241 gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa ggcagttcca
     9301 tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac cacacggtaa
     9361 atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc atcatcgctt
     9421 ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt ggcgtgcgca
     9481 acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa gcgcatttcc
     9541 cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg gagaataaat
     9601 ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc ggcgttgacc
     9661 tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg gatgcggttg
     9721 aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac ggtcgcccgg
     9781 aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg ctggagttca
     9841 accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggcggtc gatgccaaca
     9901 cgcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gaaaatttcg
     9961 accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg
    10021 cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc gctggacaac
    10081 cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc
    10141 gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga tctgaaagcg
    10201 ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg caaaattctt
    10261 tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg tgcggatatc
    10321 aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt gcagatcgaa
    10381 ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg tattcagcag
    10441 gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg tgaagccgat
    10501 atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggcctg
    10561 gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat cgccggtcgc
    10621 agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg ttccatcgcc
    10681 gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctatgaa
    10741 aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa aacgttgggc
    10801 ttcgattttg tcgagatgtc ggtagatgaa actaacgagc gcctgtcgcg cctcgactgg
    10861 agccgcgaac agcgtctggc gctggtgaat gctattgttg aaaccggcgt gcgtgtgccg
    10921 tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga cgcggtgcgg
    10981 gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt tggcattcgc
    11041 gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt
    11101 cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcgca ggtgacgctg
    11161 gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct gggttacgcg
    11221 cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg
    11281 gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc ggtgcatgtg
    11341 aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt ggtggatttc
    11401 gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg
    11461 tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgcgcat ggccaaagca
    11521 ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa gccaacatgg
    11581 atctgccgcg ctatgtgctg gtgaccttta cttggggcaa cgtcagcgct atcgaccgcg
    11641 aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg aaagcagacg
    11701 atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc ccatcttccg
    11761 acactgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc attgtccata
    11821 cccactccac tcatgccacc gcatgggcgc aggcggggct ggcgatcccg gcattaggca
    11881 ccacgcacgc tgactacttc tttggtgata tcccgtgtac gcgtggatta agcgaagaag
    11941 aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg ctgggcaacg
    12001 ccgagccgct gcatacgccg gaaattgtgg tgtatcagca cggaccgttc gcctggggga
    12061 aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg aaaatggcgt
    12121 ggattgcgcg cagtattaat ccacaactca atcatatcga cagctacctg atgaataaac
    12181 acttcatgcg taagcacggt cctaacgctt attacgggca gaagtaaaac acgcgctgcg
    12241 gaaatttcct tcctcgggag ataactggtc taattccgca gccgtttttc aaaaaaagcc
    12301 ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc tggtgctagc
    12361 gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc atcagaatta
    12421 cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt taatgcctga cgtgcttcgc
    12481 tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca tcgtccatat
    12541 ttctggcctg gtggttatta atttcaatgg ctgcccatgt atttgcactt agcaaaagca
    12601 cagccagaag ggctaaaact cgactgaaca tagatacctc ctcgacggct gactttgtgt
    12661 gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa gttgatggtg
    12721 accatttctg atgcagttgt tcaaaaaaac actgtgacgg aatgtgatga acttcaaatc
    12781 agcggtttag aggttatttg cgaaagggga gatttatttc tgctccgccc ttgagtttag
    12841 cggggtatgc cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
    12901 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
    12961 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
    13021 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
    13081 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
    13141 atgaacgttg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
    13201 gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
    13261 atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct
    13321 gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
    13381 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
    13441 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg gcaggcatgg
    13501 tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
    13561 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
    13621 agcaaaatgg ttttgcatgc cgagcagatt gaattgatag attctggaga ctagccatat
    13681 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
    13741 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
    13801 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
    13861 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
    13921 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
    13981 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
    14041 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
    14101 gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg cgctgagaaa
    14161 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
    14221 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
    14281 aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
    14341 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
    14401 gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt ctatttttcg
    14461 tcaggcatta atttcgcaag tagatccatt caccattctc ttgtgtccat aagtcaggat
    14521 ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc ggcactcgct
    14581 cgaaacctaa acccgagtag attgcggctg aatttgataa ggaacgtaga ccaattttca
    14641 aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat
    14701 tagaaacgcc tgtaacagtg tgaagttggt cgatataaag atcgattgtt ggtagttcag
    14761 atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg
    14821 tatagtctgg agtactataa ccaggtccca aaacaacctc gtgtattgtg tatttactgt
    14881 tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac
    14941 ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagct tcatggttcc
    15001 atccaaaaga attatcacca ttcattgt
//
LOCUS       DAJVRL010000092        14920 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVRL010000092 DAJVRL010000000
VERSION     DAJVRL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.092"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="OQN02_003023"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="OQN02_003023"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1643737.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OQN02_003024"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OQN02_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1643738.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="OQN02_003025"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="OQN02_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1643739.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="OQN02_003026"
     CDS             2977..3060
                     /locus_tag="OQN02_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1643740.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="OQN02_003027"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="OQN02_003027"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1643741.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="OQN02_003028"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="OQN02_003028"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1643742.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="OQN02_003029"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="OQN02_003029"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1643743.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="OQN02_003030"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="OQN02_003030"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1643744.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="OQN02_003031"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="OQN02_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="OQN02_003032"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="OQN02_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1643745.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="OQN02_003033"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="OQN02_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1643746.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="OQN02_003034"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="OQN02_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1643747.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="OQN02_003035"
     CDS             13186..14385
                     /locus_tag="OQN02_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1643748.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3713 a         3789 c         3928 g         3490 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag acgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattattcat catttttagg taatgctgcc aacttactga
    14881 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt
//
LOCUS       DAJVRL010000093        14829 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVRL010000093 DAJVRL010000000
VERSION     DAJVRL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.093"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(42..914)
                     /gene="rluF"
                     /locus_tag="OQN02_003036"
     CDS             complement(42..914)
                     /gene="rluF"
                     /locus_tag="OQN02_003036"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1643749.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1262..2209
                     /locus_tag="OQN02_003037"
     CDS             1262..2209
                     /locus_tag="OQN02_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCS1643750.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2279..3085
                     /locus_tag="OQN02_003038"
     CDS             2279..3085
                     /locus_tag="OQN02_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1643751.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3095..3502
                     /locus_tag="OQN02_003039"
                     /pseudo
     CDS             3095..3502
                     /locus_tag="OQN02_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3502..3996
                     /locus_tag="OQN02_003040"
     CDS             3502..3996
                     /locus_tag="OQN02_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCS1643752.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4062..4859
                     /locus_tag="OQN02_003041"
     CDS             4062..4859
                     /locus_tag="OQN02_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1643753.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            4870..5658
                     /locus_tag="OQN02_003042"
     CDS             4870..5658
                     /locus_tag="OQN02_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1643754.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5710..6939
                     /gene="sorE"
                     /locus_tag="OQN02_003043"
     CDS             5710..6939
                     /gene="sorE"
                     /locus_tag="OQN02_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCS1643755.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7321..8010
                     /gene="pepE"
                     /locus_tag="OQN02_003044"
     CDS             7321..8010
                     /gene="pepE"
                     /locus_tag="OQN02_003044"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS1643756.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVLLEAGHRF"
     gene            complement(8101..9732)
                     /locus_tag="OQN02_003045"
     CDS             complement(8101..9732)
                     /locus_tag="OQN02_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1643757.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9952..13635)
                     /gene="metH"
                     /locus_tag="OQN02_003046"
     CDS             complement(9952..13635)
                     /gene="metH"
                     /locus_tag="OQN02_003046"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1643758.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13835..14659
                     /gene="iclR"
                     /locus_tag="OQN02_003047"
     CDS             13835..14659
                     /gene="iclR"
                     /locus_tag="OQN02_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1643759.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3507 a         3910 c         3872 g         3540 t
ORIGIN      
        1 ccgagctctt tttggacgtt ttcatcagtc tgaggcgtta atcagcgtcc cttcttttta
       61 cgccccggcg aggtaaaacg cttaccgttg gacgccgggc gaccgccttt ttccgccgtt
      121 ttttccatct taacgactgg acgtttgatg cccgctgttt tcggtttggc cttcgcttta
      181 ggttttgcct cggaagagga attttcgatg agcttaaaga ggtcgattaa ctcatcgtcg
      241 gttaaatagc gccactcccc cagcggaatg ccgcttaagc tgacgttcat aatgcgcgta
      301 cgttccagct ttttcacttc atagccgaaa tgctcgcaca tgcgccggat ctgacggttc
      361 agcccctgca ctagggtaat gcggaagaca aacggcgctt cttttttgac tttgcacttt
      421 ttcgtcaccg tcccgaggat cggcaccccc gcccccatac cacgaataaa ttcatcggta
      481 atcggtttat cgaccgtcac cagatactct ttctcatgat cgttacccgc acggaggatc
      541 ttattcacca gatcgccgtg attggtgagg aaaatcagcc cctgggagtc tttatccagg
      601 cggccaatcg ggaacacgcg tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg
      661 ccatcttcgg tggtgctgac aataccgacg ggcttgttca gggcgataag taccaaatct
      721 tcggcttccc gaggctcaat caactgaccg tttactttca caatgtcgcc gggtttcacc
      781 tgatcgccaa tggtggctcg cttgccatta aggaacacat tgccttgctc gatatagcga
      841 tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt atttatttaa acggactgat
      901 gagtcgggca gcataaattc tcctgtaaaa cgcggaatat accgtacctc ttagcgggga
      961 aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa
     1021 aagtgtctcc ggaagtacga tgcaaatttg cctggcaaaa taaggataat caacatatta
     1081 cgaatcgctg cacacctgtg ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa
     1141 gcgctatttc tccccatgca aagagtgaaa aatcgctttg aaaggccctc atgatggggt
     1201 tatatcagat caagcggtga gaaagcgtat ttgcacaaat gtgcagaacc tggaggcggc
     1261 aatggaaaac agtgacgata tccgtttgat tgtgaagatt gcccaactct attacgaaca
     1321 ggatatgacg caggcgcaaa tcgcgcgcga actgggtatt taccgcacca ccatcagccg
     1381 cttgcttaaa cgaggccgcg atcagggaat ggtcaccatc gccatcaact atgactacaa
     1441 cgaaaatctc tggctggagc agcaactgaa gcaaaagttt ggcctgaaag acgttgtggt
     1501 ggtgtcggga aatgatgagg atgaagagac tcaactggcg atgatggggt tacacggcgc
     1561 gcaactgctg gatcgcttgc tggagcctgg cgatattgtc ggtttttcct ggggtcgcgc
     1621 ggtgagcgca ctggttgaaa acttgccgca ggcggggcaa tcgcggcagt taatctgcgt
     1681 accaattatt ggcggaccgt ccggtaaact cgaaagccgc tatcatgtaa acacattaac
     1741 ctacagcgcg gcagcgaagc tgaaagggga atcgcatctc gcggattttc cggctctgct
     1801 ggataaccca ttaattcgta atgggatcat gcagtctcag cactttaaaa ccatctctgc
     1861 ctactgggat aatctggata tcgccctggt gggaattggc tcaccggcca ttcgcgacgg
     1921 cgctaactgg catgcgtttt atggtggtga agagagtgac gacctgaatg cccgccaggt
     1981 tgctggcgat atttgctcgc gcttttttga tattcacggc gcaatggttg aaacgaatat
     2041 gagcgaaaaa acgctctcta tcgaaatgaa taaattaaag caggcgcgat attccattgg
     2101 cattgccatg agtgaagaaa aatacagcgg aattattggt gcactgcgtg gaaaatatat
     2161 taattgtctg gtaacgaata gcagcacagc tgaactatta ctgaaataaa aatcttcccg
     2221 atgtttaccg taataaaaat tacggcgggt ccctattatc taaaatcagg agtctgttat
     2281 gcaaacgtgg ttaaatttgc aggataaaat cattattgtc accggcggcg catccggtat
     2341 tggtctggcg attgtagagg aattattagc acaaggcgcg aatgtacaga tggtcgatat
     2401 tcacggtggc gatggtcaat atgaaggtca taaaggctat cagttttggc cgaccgatat
     2461 ttctagcaca aaagaggtaa atcatacggt agcggaaatt atccagcgtt ttggtcgcat
     2521 cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc
     2581 gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca
     2641 gaaaggtgtt tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg
     2701 cgtgattgtg aatgtttcct cagaaagtgg gctggaaggc tcagaaggac aaagctgtta
     2761 cgccgctacc aaagccgcgc tcaatagctt cacgcgctcc tggtcgaaag agctgggtaa
     2821 acacggtatc cgtgtggtcg gtatcgcgcc cgggattctg gaaaaaaccg ggctacgcac
     2881 gccggaatat gaagaggcgc tggcgtggac gcgcaatatc accgtcgagc agctgcgtga
     2941 aggctatacc aaaaacgcca ttcctattgg gcgcgccgga agattagcag aagtagctga
     3001 ttttgtttgt tatctgttgt ctgaacgcgc cagctacatc accggagtaa ccactaacat
     3061 tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc aacgccattt tttgtgccca
     3121 cggcaaatta gcttgcgcca tgctggaatc ggttcagatg gtttacggca acgccaatgt
     3181 tgaagccgtt gcatttgtgc ccggtgaaaa cgccggagac attgcggtaa agctggagaa
     3241 gttagtaagc attcacaatc aagatgagtg gctgattgcc gtggatttac agtgcggcag
     3301 cccgtagaat gcggcagcaa tgcttgcgat gcgcaatcct cgtttgcggg taatcagcgg
     3361 cctttctctg ccgctggcgc tcgaactggt tgataaccag gacagcatga atgttgatga
     3421 actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga aacaactggc
     3481 aacgacagag gaggatttct gatgaacatt acgctcgccc gcattgatga ccgtctgatc
     3541 cacggccagg tcactaccgt ctggtcgaaa gtggctaacg cccagcgcat tattatctgc
     3601 aatgacgaag tttataacga tgaagtgcgc cggacattat tgcgtcaggc tgcgccaccg
     3661 ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg cggtttatca caacccgcaa
     3721 tatcaggacg aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcgatggtt
     3781 cgccaggggg taaaaatagg caccctgaat attggcggca tggcctggcg gcccggtaaa
     3841 aaacagttaa ccaaagcggt ttcattagat gatgatgata ttaacgcatt tcatgagtta
     3901 aataatcttg gtgtcatact ggatttacgc gtcgtagcgt cagatccctc aatcaatatt
     3961 atcgacaaaa taaacgaaca gttaatcgca aattaaataa tagcgcctgt gtacatatgc
     4021 tttaacaggc atggatgacg acacttaaaa ggtgacaaat catggaaata agtaccctac
     4081 aaatcattgc tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat
     4141 ttcagactca tcgcccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa
     4201 aaaccggaat aatactcggc ggtacgctgg aattgatagc tctctgctgg atgaacgtcg
     4261 gcgcggcgca atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg
     4321 ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc
     4381 aggtgctgac ggtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag
     4441 cagcagaaga ggcgcggttt cgcactctcg atattctgca tgtctccgcg cttggcgtgc
     4501 aggcgctgcg cgtcgccatt ccggcactga ttgtctcact gttcgtcagc gccgatatgg
     4561 tcagcaatat gttaaacgcc attcccgaat ttgtgacccg aggactacag attgctggcg
     4621 gttttatcgt ggtggtcggt tacgccatgg tgctacgtat gatgggcgtg aaatatttga
     4681 tgcctttctt tttcctcggt ttcctcgcag gtggctacct cgatctcagc ctgctggcgt
     4741 tcggtggcgt cggcgtgatc atggccctgg tctacatcca gctaaatcca cagtggcgta
     4801 aagctgaacc tcaactccag gccaccgcct ccaccgccct tgaccaactt gacgattaac
     4861 ggagccatca tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc
     4921 tccaacctgc aacaggcgtc ctttaacttt gaacgtattc acgggctggg cttttgctac
     4981 gacatgatcc ccgccatcaa gcgactttac ccattaaaag aggatcaggt tgcggcgctc
     5041 aggctacacc tagtgttctt caataccacg ccagccgtat gtggcccggt catcggcgcg
     5101 gaaattgatg acggtaccat caacggcatc aaagtcggtc tgatgggacc attggcagga
     5161 gttggcgatc cactggtctg gggaacgctg cgcccgatta ccgccgcgct cggcgcatct
     5221 ctggcacttt cgggcaacat tctcggcccg ctgctgttct tctttatttt caacgcggtg
     5281 cgtctggcga tgaagtggta tggcctacag ctcggctttc gcaaaggggt gaatatcgtc
     5341 agcgatatgg gcgggaatgt gctgcaaaaa ctcaccgaag gcgcgtcgat tctcggactg
     5401 tttgtgatgg gcgtgctggt gaccaaatgg acgtcaatca acgtaccgtt ggtggtttca
     5461 caaacgcatg ccgccgatgg ctccaccgtc accatgaccg tgcagaacat tctcgaccaa
     5521 ctttgccctg gtttgctggc gctcggtctg acgctactaa tggttcgtct gctcaacaaa
     5581 aaaattaacc cggtatggct gattttcgcc ctgtttggct tagggattat cggcaatgct
     5641 ctgggcttcc tgtcctgatt cttcgccccg gcacgactgc cgaggccatc gctcaacatg
     5701 aggtggttta tgaaaacaac agctctgcgt ctttatggta aacgtgattt acgcctggaa
     5761 acctttgacc ttcctgaaat gcaggaggat gaaatcctcg caacggtggt cactgacagc
     5821 ctgtgcctct cttcctggaa agaggccaat ctgggtgaaa accataaaaa agtacccgac
     5881 gatgtgacga ccaacccaat catcatcggc cacgaatttt gcggcgatat tctggccgtg
     5941 ggtaaaaagt ggcagcacaa attccagccg ggtcagcgtt atgtgattca ggccaacctg
     6001 caactccccg accgcccgga ttgccccggc tactccttcc cgtgggtaga cggcgaagcg
     6061 acgcatgtgg ttattcccaa cgaggtcatg gaacaatatt gcctgctggc atacgacggc
     6121 gaaacctatt ttgaaggctc actggttgaa ccgctttcct gcgtgattgg cgcgttcaac
     6181 gccaactatc atcttcagga aggtagttat aaccacacga tggggattcg cccgcaaggg
     6241 cgcacgctga tcctcggcgg caccggacca atgggactgt tggcgattga ttatgcgctg
     6301 catggaccag ttaacccgtc gctgctcgtc attaccgata ccgacaacga caaattgagt
     6361 tatgcgcgca agcactatcc atcagaaccg caaacactga ttcattatct caatgccacc
     6421 gatgcagcat ttgatacgtt aatggcgctg agtggcggtc acggcttcga tgatattttc
     6481 gtctttgtgc ctaatgaagg actggtgact ctcgcctctt ccttgctggc gacagatggt
     6541 tgcctgaatt tcttcgccgg accgcaggat aaacatttca gcgcgccaat taatttctac
     6601 gatgtgcatt atgcatttac ccactacgtg ggcacgtcag gcggcaatac cgacgacatg
     6661 cgcgcggcgg tcaaactgat cgaagagaaa aaagtgcagg ccgcaaaagt ggtaacacat
     6721 attcttgggc tgaatgccgc gggcgaaacc acgcttgaat tgcctgccgt cggcggcggc
     6781 aaaaagctgg tgtataccgg gaaatacctg ccgctgacgt cactcacgca gattcaggat
     6841 caagcactgg cggcgattct ggcgcgtcat caggggatct ggtccggtga ggcggagcaa
     6901 tacctgctcg cccatgcaga ggcaatttcc catgattaat cgcgctactt aactctgcat
     6961 gtcgctggct ggtcgcccca gcaacttcgc gaccggatgt gcggctgatt gctgaagcag
     7021 cacaatttgc acgagaagga taaaaaatat cagcgcctta aggggcgctc aaattggtgg
     7081 caaagtacac attgtttatg ccggatgcgg aatgaagcct tattcggtct acataatttt
     7141 gaaaatccaa tatattgcag ttatctggta ggccaggtaa gcgtagcgca tcaggcaatg
     7201 ctgtgtttgt catcagtctc agcgcccggt gcaatacgtg acatcgtgag ccatgtcagt
     7261 aagtggttaa attcgcagtg gtagcatgcc ttccgtgatt tcttactaac cggagaagta
     7321 atggaactgc ttttattgag taactcgacg ctgccgggta aagcctggct ggaacatgca
     7381 ctgccgctaa ttgctgaaca gttgcagggt cgccgctcag cggtgtttat ccctttcgct
     7441 ggcgtaacgc agacctggga tgattacaca gagaaaacgg ctgcggttct cgctccgctg
     7501 ggtgtttctg tcaccggtat tcatagcgtt gtcgatcccg ttgccgcgat tgaaaatgcg
     7561 gaaattgtga ttgtcggcgg cggtaatact ttccagttgc tgaaacagtg ccgcgagcgc
     7621 gggctgctgg ctccaattac tgacgtggtt aaacgtggcg cgctgtatat tggctggagc
     7681 gcaggcgcta accttgcttg cccaaccatt cgtaccacca acgatatgcc gattgtcgat
     7741 ccgcaaggtt tcgatgcgct aaatctgttc ccgttgcaaa tcaacccgca cttcaccaac
     7801 gcgctgccgg aaggccataa aggtgaaacc cgtgagcagc gtattcgcga actgctggtc
     7861 gtcgcgccag aactgacgat tattggtctg ccggaaggta actggatcac agtgagtaaa
     7921 ggtcacgcta cgctgggtgg cccgaacacc acttatgtgt ttaaggctgg tgaagaagcg
     7981 gttctgctgg aagctggtca ccgtttttaa gtgaattgcc ggatgcatga tccggcatct
     8041 tgttcatagt gcctgatgcg acgcttgacg cgtcttatca ggcctacagg attcacaata
     8101 ttaatactcg tcccgtcctt catcttcatc cggctgttcc agcacactgt aagccaccga
     8161 acaaaacagc gagttcaggc gctgcatatc tcccagtaag cctaaatgta gcgaactggt
     8221 ttcaatgctc tgtacgtttt gctgatgcag gcgatcgacg tgggcgtggg aatagcggcg
     8281 attaagaatg cgaaaacgat gtttgctgcg acgcaaacga cgagcgctgg tgacatcgcc
     8341 agagaagaac accgacattg ccagctttaa attactgagc aattgctcat agagcgcatc
     8401 cagttccttc aacccatcaa gcgaaaatgc ccgccgtgct gccagcgatt tatcggcaat
     8461 ttcgctgccc atgcgctcga cgatatcgga ggcctgttca aggttgagcg acatttcgat
     8521 gatctccgcc cagcggcgcg actcttcctc cgccaactct tcttttggca tccgcgccag
     8581 atacagctta atggcggtgt agagaacgtt gatatcatct gccagcttac gcagctcttt
     8641 ctcctgccgt ggctcgccgt gcatcacttt attcagccct tccatcatct gttccatggc
     8701 gtcgccaatg cgcagggttt cgcgcgcggc gttcgccaga gcaagcgtgg gcgtgtccag
     8761 cgcgctgaca tccagatgtt taggccgtag ctgggtatcc agttccggtt catcgcgaat
     8821 aatcgttttg caaaaccgtg ccatcgggtc aacaaatggc agcatgacca ggcaacgtac
     8881 aaggttgtag aagacgtgga aatagatcac cagttccgct tttggcaatg acagcttccc
     8941 cattgtctct gccagcaaat ggacaaacgg caggataatc aggctaccca ccagcttaaa
     9001 cagcagacta cccagcgcga cacggcgggc tgcggcattg gcggcactgt tgttgagcat
     9061 cgccagcagg ccggaaccga ggttagctcc aatcaccaga cagagcgcca cggggaagga
     9121 gataatgcct gcagcggtca gagtcgcggt cagcagtaca gcagcaaggc tggagtagct
     9181 gataatggcg aacatcgcgc caatcagcgc atccagcaga atatcgccgg tcagcgaggc
     9241 aaagatcacc tgaacgccgt ttgcctgggt gatcggcgtt acggcctgca caatcaactc
     9301 cagcgccagc aaaatcagcc caagaccaat accgacgcgg cccagttgcc cggcgcgtga
     9361 ctgtttgcgt ccgaggaaaa aaatcacgcc gataaaaata agtaacggtg acagccagga
     9421 taagtcgaag gtgagaatac gcgccattag cgccgtcccg acatctgccc ccagcacaat
     9481 gaccagagcc ggtgcgaggg ctaccagatc ctgagcgacg aacgaggtca ccagcatggt
     9541 ggtggcatta ctgctctgta ccagtgcggt aacgccgatc cccgcgcaaa aggcgagcgg
     9601 ctttttttcg acgctccggc taaggacagt acgcaaacgc gcgccgaaga cgcgcattac
     9661 gccggttcga acaatatgag tcccccagac cagcagggcg acggcagaaa gcaggtgaag
     9721 cagcgttaac acgagttcag gttctcctta tcgttatatg ttcctgaagc aattgaatga
     9781 ccaaaggacc ggaaacacgc tgactggcat ctttactgtg ccttttgaca ttcgtttttc
     9841 cggctaagat tattccagcc tgtttccagt ataagtgttt catattaata aagagacagg
     9901 gcactcatta agcgccctgt ttgttgtaag gaaaagtgac agatttgtga atcagtccgc
     9961 gtcatacccc agattcggtg ccagccagcg ctcaacatcg gagacgctca tacctttacg
    10021 gcgggcatag tcttcaacct gatcgcgctg aatttgcgcc accgcgtagt acttgctgtc
    10081 cggatgactg aaataccaac ccgaaaccga tgcaccaggc cacatcgcga aagattctgt
    10141 gagtttcatg ccagtatgtt tttccacttc cagcagctcc cagatggtgg ctttttccgt
    10201 atgttccggg caggccggat agcccggtgc cggacggatg ccctggtagt tttcgcggat
    10261 cagttcttcg ttgctgaggt tctcgttcgg cgcatagccc cagtagactt tacgcacacg
    10321 ctcatggaga tactccgcaa aggcttcggc taaacggtcg gcaagcgctt tcaccatgat
    10381 tttgttgtaa tcatcgtgct gcgcttcaaa ggcatcagcc agtgcgtcct cttccagccc
    10441 gccagtcacg gcaaatgcgc cgatgtaatc tgctttacca gaaagcttcg gcgcaacgaa
    10501 gtcagcgaga cagtagttag cgaatcccgt tttttcggtc tgttgacgca gatggtggct
    10561 gacgttgatc acatgggtgc gcgtttcgtc acggtagatt tcaatgtcat cgcccacacg
    10621 gtttgccggg aacaggccca ccacgccacg cggattcagc gttttctcgg cgcttaattt
    10681 atccagcatg tcgttggcgt ctttaaacag ccgctgcgcc tcaacgccca ccacttcatc
    10741 ttccagaatg cgcggatact tcccggccag cgaccaggtc ataaagaacg gtgtccagtc
    10801 gatgtaatta cgcagcgttt cgatgctggc ttccacttcc tgcacgccga gacggtgcgc
    10861 caccggcggc gtgtaagcct gccagtcaaa tgcgaaatcg ttatcgcgcg ccgcttccag
    10921 cgtgaccggt ggtgtgcgtg gtttcttgcg cccgtgctga atacgcacgg tttcgtactc
    10981 cttgcaggta cgagcgacaa aatcatcacg ctgagtatcg gaaagcagcg ccgctaccac
    11041 accaacggtg cgcgaggcat tctgcacata caccgtaggg ccgctgtagt tctgctcgat
    11101 tttcaccgcc gtgtgcgctt ttgaggtggt cgcgccgcca atcagtaacg gaatggcgaa
    11161 gccctgacgc tccatctctt tcgccacgtt aaccatctcg tccagcgacg gcgtgataag
    11221 ccccgaaagg ccaatcaaat cagcattcac ttctttggcg atacggagaa ttttttccgc
    11281 aggcaccata acgccgagat cgacaatttc gtagttgtta cattgcagca ccacaccaac
    11341 gatattttta ccgatgtcgt ggacatcgcc tttcacggtg gcgatcacca tcttgccgtt
    11401 ggttttaccc tgctctttgc tggcttcaat aaacggttcg agatacgcca ctgcctgttt
    11461 catgacgcgc gccgatttga ccacctgtgg caggaacatt ttgccttcgc caaacagatc
    11521 gccgaccacg ttcatgccgt ccatcaacgg gccttcaatc acttcaatcg ggcgcgtagc
    11581 ctgctggcgg gcttcttcag tatcctgctc gataaactcg gttatgcctt tgaccagcga
    11641 gtattccaga cgtttattca cgtcccacga gcgccactcc gcttgctggg cgttagcagt
    11701 gtcgtccgct ttactgccgc gatatttctc ggcaagctcc agtaaacgct cagtgccatc
    11761 gtcacgacga ttaagaatca catcttccac cgcgtcgcgc agttcagcgg gtaggtcgtc
    11821 gtaaatcgcc agttgcccgg cgttgacgat ccccatatcc atgccattgc gaatagcgta
    11881 gtagaggaac actgcgtgaa tggcttcgcg caccggatcg ttgccacgga atgagaaaga
    11941 aacgttagat acgccgccgg aaatcagcgc gtgcggcagt tcgcgtttga tgtcttcaca
    12001 cgcgccgata aagtcctgcg cgtagttgtt gtgctcttca ataccggtgg cgaccgcgaa
    12061 gatgttcggg tcgaagatga tatcttctgg cgggaagcca acctcttcgg tgaggatttt
    12121 gtacgcccga cggcaaatct cgattttccg tgcgcgagta tcggcctgtc cctgttcgtc
    12181 aaaggccatc accaccactg ccgcaccgta gcgacgcaac aatttcgcgt gatggataaa
    12241 ggcgtcgacg ccctctttca tcgagataga gttaacaatg cctttgccct ggatacactt
    12301 cagacctttt tcaatgacat cccattttga ggagtcgatc ataatcggca cgcgagcgat
    12361 atccggttca ccggcaatca gattgagaaa acgcaccatc gccgcttcgg catcgagcat
    12421 cccttcatcc atgttgatat cgataatctg cgcgccgttt tccacctgct gacgcgccac
    12481 atccagcgcc tcgctgtatt tctcttcttt gatcaggcgc ttgaacttag cagagccggt
    12541 gacgttggtg cgttcaccca cgttcacaaa caggctatcg tcgccaatgt tcagcggctc
    12601 caggccagac aaacggcagg ctacgggaat ttccggcagt ttgcgcggcg ctaatccttc
    12661 tactgcacga ctcatcgctg caatatgttg cggcgtggtc ccgcagcaac caccgacgat
    12721 attgagaaag cccgcttgcg cccattcacg tatctgtttt gccatcgtgt cggcgtcgag
    12781 atcgtattca ccaaaggcgt taggcagccc ggcgttcggg tgggcggtga cgtagcattc
    12841 cgcaatccgt gatagttcct gcacgtactg gcgcagttca tcgggcccca gcgcacagtt
    12901 caggccaaag gtcagcgctt cggcgtggcg caatgagttg taaaatgctt cggtggtctg
    12961 cccggagagc gtgcgcccgg aggcgtcggt gatggtgccg gagatcataa tcggcagctc
    13021 aacgcccagc gcttcaaact ccgttttcac cgcaaagacc gccgctttgg cgttaagggt
    13081 gtcgaaaacg gtttcaatca ggatcagatc cgcaccacct tccaccagcg ctttggtgga
    13141 ttcgcgatag gccgccgcca gctggtcaaa agtgatgttg cggaatgccg gatcgttgac
    13201 gtccggagaa atagacgccg tgcggttggt tgggccaaga acaccggcaa cgtagcgcgg
    13261 tttctccggc gtgcgcgcgg tccactcgtc agcacaggcg cgcgccagtt tcgccgccgc
    13321 aaagttgatt tccgccgaca gggattccat ctggtaatcc gccatcgcaa tggtcgtgga
    13381 gttgaaggtg ttggtttcga tgatatccgc gcccgcttca aagtaggcgt tgtggatagc
    13441 ggcgatcact tccggtttac tgagtaccag caggtcgttg ttgcctttga ggtcgcatgg
    13501 ccagtcggca aagcgttcac cacgaaaatc ggcttcgttc agtcgatagc tctggatcat
    13561 ggtgcccata ccgccgtcca acaccagaat acgttcattt aactgcgcac gcagttgttc
    13621 cactttgctg ctcacacttg ctcccgacac gctcaaccca gatttaataa aaattcaaca
    13681 aaccatactg gaataaacgc atctgtggta aaagcgacca ccacgcaaca tgagatttgt
    13741 tcaacattaa ctcatcggat cagttcagca actattgcat tagctaacaa taaaaatgaa
    13801 aataatttcc acgatacaga aaaaggaggc tgtcatggtc gcacccattc ccgcgaaacg
    13861 cggcagaaaa cccgccgttg ccaccgcacc agcgactgga caggttcagt ctttaacgcg
    13921 tggcctgaaa ttactggagt ggattgccga atccaatggc agcgtggcac tcacagagct
    13981 gtcgcaacaa gccgggttac ccaattccac gacccaccgc ctgctaacca cgatgcaaca
    14041 gcagggtttc gtgcgtcagg tcggcgaact gggacactgg gcaatcggcg cacatgcctt
    14101 tatggtcggc agcagctttc tccagagccg taatttgtta gcaattgttc accctatcct
    14161 gcgcaatcta atggaagagt ctggcgaaac ggtcaatatg gcggtgcttg atcaaagcga
    14221 tcacgaagcg attattatcg accaggtaca gtgtacccac ctgatgcgaa tgtccgcgcc
    14281 tatcggcggt aaattgccga tgcacgcttc aggtgcgggt aaagcctttt tagcccaact
    14341 gagcgaagaa caggtgacga agctgctgca ccgcaaaggg ttacatgcct atacccacgc
    14401 aactctggtg tctcctgtgc atttaaaaga agatctcgcc caaacgcgca aacggggtta
    14461 ttcatttgac gatgaggaac atgcgctggg gctacgttgc cttgcagcgt gtattttcga
    14521 tgagcaccgc gaaccgtttg ccgcaatttc tatttccgga ccgatttcac gtattaccga
    14581 tgatcgcgtg accgagtttg gcgcgatggt gattaaagcg gcgaaggaag tgacgctggc
    14641 gtacggtgga atgcgctgac tttttctggc gggcagaggc aatattctgc ccatcatacc
    14701 tgagtggcaa tagaataagg gtgtctgtta atcgcattga cgccaaaata acttaatgtc
    14761 atacacttca cacactttta ttaactcagc attattttta aacatcaaac cacttaatta
    14821 ttaacaaaa
//
LOCUS       DAJVRL010000094        14569 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVRL010000094 DAJVRL010000000
VERSION     DAJVRL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.094"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..707)
                     /locus_tag="OQN02_003048"
     CDS             complement(<1..707)
                     /locus_tag="OQN02_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1643760.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNV"
     gene            complement(707..1387)
                     /locus_tag="OQN02_003049"
     CDS             complement(707..1387)
                     /locus_tag="OQN02_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1643761.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(1384..2583)
                     /locus_tag="OQN02_003050"
     CDS             complement(1384..2583)
                     /locus_tag="OQN02_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1643762.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(2584..2937)
                     /locus_tag="OQN02_003051"
     CDS             complement(2584..2937)
                     /locus_tag="OQN02_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643763.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(2937..3677)
                     /locus_tag="OQN02_003052"
     CDS             complement(2937..3677)
                     /locus_tag="OQN02_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCS1643764.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            3900..4190
                     /locus_tag="OQN02_003053"
     CDS             3900..4190
                     /locus_tag="OQN02_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HCS1643765.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(4214..4558)
                     /locus_tag="OQN02_003054"
     CDS             complement(4214..4558)
                     /locus_tag="OQN02_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643766.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(4571..5635)
                     /locus_tag="OQN02_003055"
     CDS             complement(4571..5635)
                     /locus_tag="OQN02_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643767.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(5638..5943)
                     /locus_tag="OQN02_003056"
     CDS             complement(5638..5943)
                     /locus_tag="OQN02_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643768.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(5945..6547)
                     /locus_tag="OQN02_003057"
     CDS             complement(5945..6547)
                     /locus_tag="OQN02_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643769.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(6547..8562)
                     /locus_tag="OQN02_003058"
     CDS             complement(6547..8562)
                     /locus_tag="OQN02_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCS1643770.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(8740..9165)
                     /locus_tag="OQN02_003059"
     CDS             complement(8740..9165)
                     /locus_tag="OQN02_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643771.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(9169..9609)
                     /locus_tag="OQN02_003060"
     CDS             complement(9169..9609)
                     /locus_tag="OQN02_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HCS1643772.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(9620..10765)
                     /locus_tag="OQN02_003061"
     CDS             complement(9620..10765)
                     /locus_tag="OQN02_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HCS1643773.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(10769..11326)
                     /locus_tag="OQN02_003062"
     CDS             complement(10769..11326)
                     /locus_tag="OQN02_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643774.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(11301..11690)
                     /locus_tag="OQN02_003063"
     CDS             complement(11301..11690)
                     /locus_tag="OQN02_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HCS1643775.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(11677..12231)
                     /locus_tag="OQN02_003064"
     CDS             complement(11677..12231)
                     /locus_tag="OQN02_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643776.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(12228..12635)
                     /locus_tag="OQN02_003065"
     CDS             complement(12228..12635)
                     /locus_tag="OQN02_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCS1643777.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            12634..12868
                     /locus_tag="OQN02_003066"
                     /pseudo
     CDS             12634..12868
                     /locus_tag="OQN02_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12865..13806)
                     /locus_tag="OQN02_003067"
     CDS             complement(12865..13806)
                     /locus_tag="OQN02_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCS1643778.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(13818..14321)
                     /locus_tag="OQN02_003068"
     CDS             complement(13818..14321)
                     /locus_tag="OQN02_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643779.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(14326..>14569)
                     /locus_tag="OQN02_003069"
     CDS             complement(14326..>14569)
                     /locus_tag="OQN02_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643780.1"
                     /translation="FKREVLASADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKG
                     RNTRSTTGDAQRPNMGMTSIASLNKQNAEFWSNRKG"
BASE COUNT         3326 a         4045 c         3670 g         3528 t
ORIGIN      
        1 tggacatttg tcgccgatac agtcggctgc cctgacgcgc cagtaacgtt caatgtaact
       61 gtgacagcaa ggactcttga gaatgtcatc gggaacctca tcccgtatgt tgaagataca
      121 ttcggggagc cattcaactg accgcactgt aaaattaatc cagacggaaa tttttgcgca
      181 gcagccgttg cggtattact tgacgcgaac aggctcatat caggtatctg attcgcccct
      241 gtacctacat tccttttagc cgcttctccc aaaccaaggt atgtgagaat gtcagcaata
      301 gtatttttcc caataatgtc acggccaaca gaagttaaat cagtctgcgc tgctgtatca
      361 tttccagtga aatatgggag tttatttgca cctgttgcga gcccagctaa tgccgacagc
      421 gtggcatcaa gcgcctggaa atctttcccg aaagcggtcc ccattttgga tataaacccg
      481 ttcaggtctc catcatcaag cacatccagc ctgcttttgt tggcggtgta ctgcgccaac
      541 gctgccgcga taaagctggc ttgccgaata gctttgttga cctgtgcgct ggatgcttta
      601 cctgccgtaa accctgaaag cagagccgga agtgcttccc agtcagcttg tgaggtgacg
      661 ttagcgttcg gatcaagcgc gaaaggttta aagttgttta ttgccattag agtattgtcc
      721 cccatgctcc aacatcgaac ccgccgatgt attcgttatc catatcaaac ccaaagaatt
      781 tagagccctc ggatggtgct tctaccgaag gcgtttcaac atcaccggcc catacgccag
      841 ctgctttaac ggtgagatag ccctgtttga tagcggcaat cagttcaaga gacacatcag
      901 aaatatcagt ctcggggaat acccaaaccg atatcgtcat gtcctggttg tcgacaattt
      961 gcatcctcag gcctgagcct tcagtcgcag cgtcaaggat gggaggcaga gagtcgttcc
     1021 gaccgtccca gttgttgata gcgattttcg ctttcagaat gatgcggtac gtctcatcgc
     1081 ttagcgtcgt atagccagaa tcaggatcat atggcccttt ccagatgccc tggtcatacc
     1141 caagcccgtc agtgtcccag ctgaaataaa ctccgctaat tggctggctg actatgcgac
     1201 tgcgtccgat ccacagaccg aggatgtcga gctgtacacc gacagcagta tcaatatcga
     1261 aggctgttat aagccctgac atagtgctgg acacatcaat cagcgggcgg gtgctcagat
     1321 ctatatggtc aaaaaagagt ggcttggtag cgtggtagtt agtgatcagt tcggtgtatt
     1381 tgctcatgag gtcaccgtga tactgatatt cgcggtgcta caggacgcag aagcatcata
     1441 ggcaatatca atgtttgatg ccgatacgct gccagacgac ttaccgatca gcaggtcggt
     1501 aatatcgtaa tagcgggcat tcccgccgct cacaacgccg aggtttgccg gggaataaat
     1561 acggctcagc agaacgtcgt cgccaattgt taggccattt atataatcgg caacagcctg
     1621 tttaatctgc tcgccgattt gagaggtata gccggtaaaa actttcaggg taatggctac
     1681 gaaaattggc acatcggtag agcgcgaaaa actgatgacg tgtggattac cgtaagtatc
     1741 cggcaccgtg acagaagttt taccgtaagt tgcggttccc tgccctttac tcccccggat
     1801 tgtttgggct atctcggtaa catcccctcc atcgacgatg gcggaaatag agtgtggcgg
     1861 cagcccgttg ctgtcggttg ccccagtgtc gttctcatat agtttgtgac gtgtcacgcc
     1921 agcaacatta gcgatagcac cgtcgacacc ttcaaacggt gtgatcgatg gtagcgcgac
     1981 gctttgcccc tgccgaatgc gcagctctgc gtcggtttcg gctggtgaac cgacagtagc
     2041 cgcaactgga ttggttaccg acacccaacc tcgggtcggg gtgttgatag tggtaatagt
     2101 cccggccagc gccgcaaccg aaccgctatt cgcacatgtg gccgtcacca gcacagtacc
     2161 atcaacgccg atcgctacac ttgcgggaaa attccagata atgccgtttt tatcccgtgc
     2221 ggagccattc gtgatagtcg tgcctgccgt accggttaac agaaggtcag cagtagagtt
     2281 tgtcgctact tttcgcgtga tcccgttaat tttcacattg ctgctaagcg ctgcggcctg
     2341 cgctgtcgtc ggtgaaaaag agttgtagat cccgatagcg gtgttgttag cgtcatgcac
     2401 ggcaagagcc accagcgcga ccatctgccc gtctttgctg tctggttcga ggtaggcatc
     2461 actaccgtaa atctgcctga aatagctggt cagtgtatct aggattgtct ggtaatcagg
     2521 cgcactaatc ccctgggcgg ttaccgttgc cgatagcccc agcgtgtcga ggttcaaagc
     2581 catttatgcc tcgcttgtta cagtcgtctg gccgtagatt gtgtcaatgg aggaagtgaa
     2641 ggtgacgcga cggctggtgc cgtcataatt ggtatcgaag gaaagaatcg acagaacgcc
     2701 cggtgtatcc tgtatgcgtt cgcgtatagc caggatgtag acatctgatc gctgcttccc
     2761 aagcactgac tgaacatacg gcgtgccttc cgtcagatcg agaaaccact gaccgcgcca
     2821 cagctcgaaa cgggttttta cggcctgggc gacacattcc ggactgtcga taaggaaagt
     2881 atcgtcaccc tggccgaaag tgtaatcgcc ttcagtatct tcgcgacggt atcgcattat
     2941 tgcggccctc cggtagttcc cccgccagtc tgtactccgc catgtttatg cgtggcgaca
     3001 ctgataccag aagctgttac gtcattcgtt accatcaccg gcccaagcat cgtcgcagta
     3061 ccgccgcttt cgcccattcc ctgagacaag ttgccattaa tcgttacgtt gccgttcagc
     3121 gtgatagtcg gggatgtgat tgtcgttcca ccttcagccg tagctgtaag ctggcccggt
     3181 gttttaatgg tgatgttatg tcctgcggcg acttctacaa acgccgcacc atcatcggtt
     3241 cgcagctgcg cggcgctggt actgataccg ctgattttcc gtgcttgcga ctgcgggcca
     3301 acgatggcga acgcatcaga taagtcatgc tggcgcgggt cgacggtctc ctgaacgccg
     3361 ccgctctgcc accaaaaatc gatgcaacgg tcagcaaaaa tcaggaggca ctcgtcgcct
     3421 tcttttaccg gaaaggtcag cgtgcaaccg ccgccgcgcg ggaagatgac cggcacatcc
     3481 accagcggtt ttaattcggt ggatccatcg ccaacaatac cgcgtagcgc cacctctact
     3541 gtgcaggtaa cagtctcagg atcgaacgac tgaatgatgc cgggcatcgc tacgcgcatc
     3601 tgggtagaca ccgaatcggc aatggcctgc gcggtctgct gctcaccgcc gatctgtgat
     3661 tgagttggaa ttggcataaa acccccataa aaaaaccagc cgaagctggt ttggtatcaa
     3721 ttatctctta gtaactaagc tacatcagtg tgaagtaagg cttttccgct gcaatggcac
     3781 acattcacaa ggtgtattag gatatgtttg caacaagtaa gcattgaaaa tcaatttcag
     3841 ccatgaagcc gccatagagt ggacctgcac catcgcatta ttgactacag gagtaacaaa
     3901 tgggatttag atttcgtaaa agaatccgga ttgcgcccgg actcgcgatc aacattagta
     3961 aaagcggagt aagcacttca attggtggta aaggttccac cattaacatc gggaaaaaag
     4021 gcgtaaagat gacaaatggc cttcccggca cggggctgtc gcataccact aacctttact
     4081 ctcccgggaa atcgacagaa aaaaaacaac ttactcataa gcaaaaaata attagaaaca
     4141 tcctgttcgt aattattgtg tttataataa ttaaagctca atatttttga cgcatgcccg
     4201 cctatctggc gggctatttt acttttcggc agtcgtatgt tgcatactga cgcggcgaat
     4261 ccatgctggc ttgcagccac tgtgcgttga gaatggcttt gccgtttcga ttgatgtact
     4321 caagaccaac ccatcttcca ggctgatcgg tagccatacg ccaatccatc ttgatattgt
     4381 tataatcgcc tttgtttttc aggaaggtga ttttttgcat ttctggcttt gcgccattga
     4441 ttctggctaa gccatcatct gcccaatgga ttttaaaatc accacattgc tgatccgcaa
     4501 aagcgggtgc tgatataagc accgcgaaca cggtaacagt gcaaagtaaa tgtctcacgg
     4561 ccccacctca ctaattcgtc gtcccttgca ttgctttcgg gctataaaga tcacgagcgc
     4621 cacgcgcaaa acacatcaaa tccatgtacc acgcctgacc tctggtgtcg ccagtatagt
     4681 cgatagcttt gacgatataa acgccatccg tcgcaatgct ggcagcctgt gacgtcgtgc
     4741 cggtcagcac acggttgccg ttctcttctg tttcggtgat acgcccgggc gactgtgcta
     4801 tttcgctatt gccgagcgtg gcgcggtaca ccgaagcctg atcgagctgg ataagaccat
     4861 taatacggat gtttgggttt atcaggcatc gcacgtttac tccgccaccc atcgtttgtt
     4921 gcggcatacc gatcaggcca gtattggcat tcaacacaat ggcttcgtga atatatttat
     4981 cctccggcac catctggacc tgaccatcca ccagttgcca tgtcgctttg cactgcgcag
     5041 caatattatc catcacgttg cggctggatg agtaaatcgc gcggccacga ggaaacacgg
     5101 tatcaggaaa atcgccggta atgccctgtg tcacgccgaa cgcgttgaaa tcctgcatcg
     5161 tcgcccggtg cagatccgca acggtatatc cagcggcaag cgtggtgatg gtggtcgcat
     5221 agaggaacgc ttcgtgatca ccaatggcct gaatcaacac ccaggaatcc gtaatgttgt
     5281 ccttcccggt gacggtgaag cgaatatcac cgtcaaaaat caggccgtag ttctgaccgt
     5341 tcacctgccc tatctggtct ggtgaaatct cccgggacac accaacctgg ctcgcatcaa
     5401 catccggcgc tataccgtca tacccggcaa tgatgcgaat tttggcaaac tcctgcccaa
     5461 gtatcttgtt cgtggtatcg gtcgaaaggt tgtaaatttt cacgttcgcc acgcgcggcc
     5521 agcgtgtgtc tgcccactcg atctggaacg tgaccttaaa gtcagacagg gaaacgccct
     5581 gcccgttctg gtccaacagt tgcagctcaa aatggcgcat ccagttaaga gacatttcta
     5641 ctcctgtacg aaaatgaggt ggctgtatgt gccgaggttg gttttggtgg gctcgtctgg
     5701 tgcgccctta tcggtcgcca ccaccagcgc gccatcaatg ccaagctgtg gatattgtcg
     5761 taaaaggttt acgccggtca gtagaggtac gccagagaga agcgcggcac cgccgctatc
     5821 catcacgtcc ataatccagc cagccgcatc acgccagatg atcctgagtg tatacgtggt
     5881 attgcccagc aaaactcgga actgctgatt gtcaggagaa agcggtattt cgttaaactg
     5941 catatcatcc cccgaatgct gatgtaacgc ttccgcccag ctggctcagc aaggattcgt
     6001 ttggcggtgt agtggatttc atcccggaat tctgcaccgc cgatgtgctg acgccatcct
     6061 gcatatctga tttatctgca acgctaacgc tctgcgtctg cgacatgatc acttcacgca
     6121 gggtaagcgt gcagttcagc acgttctcgc tggttttatc cgttgtcacc tcgatggctc
     6181 gcaccagcat attgctgtaa acccgctttc cggtcactac atcgaacggc accctagagg
     6241 actggagatc cagtagttgc tgataggtct ctttcgggct aagtccggcg ctgaggccga
     6301 ttgacgatgt atcaatgaag tccagcaacg aaccgccacc tgcgaagccg cattccatcg
     6361 tgacttcgct ggggcgctta tacgcatgat cggcgatgaa acccgacgcg ctattcgttg
     6421 ttggcttctc caccgggtgc tcagtaattt cgagcgcatc agaatgcttt tcagaaacaa
     6481 ccacgctggg gattaatatt ccaattcgcc gggattgctg gcgaaaaatt gctgataaaa
     6541 tatccattat ctcggtcctg cggggagttg ctgggttaac tgtgaattca cgcccttttg
     6601 acggtcaaca gtcaaacggg cagcctcgcg cggatcgaaa acgccgtgga tgttaatatt
     6661 cgtttcctgc tgaatcaccg gggcgctggt gggcatattg ctcattactt tcggaatgta
     6721 gttgcgcgtt tcctgcggca ttagccccat tccataacgc ttaacattcc cgatccccca
     6781 gttatatgat gccagtgctt tgctaaggtc tccgccgttc tgccgcaaca gctggctcag
     6841 gtacttagcg gctgcctgag ctgacttttc cgggtcgaat acatcattcc cacgcaggcc
     6901 catgtcgcgc gccgtgccat ccataaactg aaacagacct ttagcgccag cgcctgaaac
     6961 ggcgaactga ttaccgcccg attccgttat ggccacgctg cgcaataacc cctccggaag
     7021 ccggtagagc tgttccaggt tggtaaacat cggctgcatc catcccagca gttcagagcc
     7081 tgctttggtt ggctgtggcc gcttaactga ctggccgagc tgctcaggct catcatcacc
     7141 gaaccagccg cgcaccgttc ggcccacgct gcgaggatcg aatccccagt gctctttaat
     7201 ccagtcggcg gttccgttgg cgctgtctgt taccatcggc atcgctgacg gattttcgct
     7261 gccctgatta agcatctgtt tgccgatgct ggcggcatca gcccagcggc catctttaat
     7321 ggcgttgagc aggtcggcga tcatgttcag catcttgccg aactcaccca tttgcgaaat
     7381 gaagttgcta aaatcccact ttagggacca tgacttgggg tcaataccga gcaacttagc
     7441 cagcgctttt gtcaggtccg taacagtctt tttcaggtca ccaatcattt tgacggcctg
     7501 atcaatttca gtcttccatt tcccccagtc aatcaggctt ttgccacctt ctttccacgt
     7561 ctggtaatca tccaagagca gaccaagtgc aataataagc gtcgttatca tgcccaaggg
     7621 tgatgacatg aaagcggtat ttagcagccg ccatgcaacc aacagcccac tgaacaatgc
     7681 gataagctgc tgcgtggctg ggttcagctt tttgaaccag gcgatcacac cctcaacagc
     7741 ttgcccagtt cgccataaca cgcgggtaat cgcgtcacct gcccagagaa ttcctttgat
     7801 gatcttcgtg atgaccgctt caatcttcgg ccagttgtcg agtatctgcc tgcggaagtt
     7861 atcaatactg ccagcaaggc caccagctag gttagagcca atcttgtctt tcgcctgtcc
     7921 aagcgtcatc gtcagattac gcatggaggt catgaaaata ttggactgtt tagccgctga
     7981 ctcagcatta aaaccaatac gctttgccgt caacgcgtac tcagaactga gctgccccat
     8041 ccctctgcgc atcgccatca gcgtgttttc atcgatgccg agcatctgcg cgtattgctt
     8101 cgcgcggtaa tacggcatgt tgttgagctt ttggccaacg ccagtaaaga tggcagcagt
     8161 atcacgcatc tttccgctgg catcgcgggt ctggacacca agacggttca ggaacccttc
     8221 cgcccccgga ttgctacgca tgaagccagc caacccttcg agggaggaca tagccgactc
     8281 ggcgctggca ccggtttgcg atgcggcgta tcccagcgct ttgatgccct ggacactggc
     8341 ccccgtccgc tgggatgccc agtaaatttt atccagaccg ttcgcgatct gagtggtaaa
     8401 tccgacaatg ctcagcgctg cgcctttcac caccgcgccg accttcagaa cgttcgcggt
     8461 aacgcctttc agcacggctt caaacttatt agcgccagcc tgatcgatat cgaatcccag
     8521 cgaaacaagg aaatctttaa tcgtatctgc gttaccgctc attggccgct ctccatttat
     8581 ctacccgggc gtcgttatcc tcgcgcatgt cgaggtagtc attgagaagc gcgatgcggc
     8641 aaagatctac cgcaccgctg ttaaggtctt tctggtcaat atggaaggca agcgccgggc
     8701 gaagaataaa atcttcaccg cccggcaggc tgttgaaggt tattccgcta gcggggtggg
     8761 cgtctcgctg gtagggagtc cttgcaaaaa atttcccagc gagtcggcga ccacccgcgc
     8821 caccagttgc agcatggtaa gcaggtcgat atcgtcaaac gccatttcgc catgctggca
     8881 gaccggcacc cagcctttca tgtgctcgcg tgaaacaacg gaaagacagg ggaacaggat
     8941 agcgtccacg tcgccatcac tcagatcgga cacagcattg gcaatctttg gcaggatagt
     9001 agccatcgcg ccttcggtgt ctttgctgct gatcttctcc tgaacgctcc ggaagtccga
     9061 aaccatcccg gccagaaccg gcaacagctt gcgggacacc ttcagctgtt cgaaaacgct
     9121 gagctttgcg gtgcgatatt tcacgccttt aatttcgaat tccatgcgtt aaaactcccc
     9181 gagaagctgg tcaatcttgc cgcagtcgaa tacccaggcg acggttccgc cctctttagc
     9241 gttattgaaa tcaggctgtt tctggaatgc acacgaacgc gcagtagaaa tatcacccga
     9301 tgccgtgttg cgaatgacga tcacgttatt gccccaggtg gcagaggact ggctttgcgc
     9361 gttatacgcc agagacagct ttttattcac cggggaggtt ttgagtagcg tcaccgtaat
     9421 ggtgcctgac ttatcggcgt gcaggctgtg catcacctcg ccatcggcac cgatagtcat
     9481 ggtgtttttg ttgccgccca tggtctgggt gataccttcc tcagagttcg cagaaccctg
     9541 accaagatcg ataacgccgg tcggcccggt gagcgacgcg gttacatcga gaaaagaata
     9601 agttgccatt tatcgctcct tagcgaacca cgttgatctg cacatcggca taatgaactg
     9661 cgccagccag cttacaggcc acctggatta acggtgcttt gcgcgcttca cggtcggcct
     9721 gcgcctgttc ggacagcggt tgcgcataca cgtaatagcc tttggtcagc gtatcgccgg
     9781 aattcagctg tccgataggg ccaccattcc acacgccagc agctaccaga ccgttcgtga
     9841 cggactgatc catggactgt tcaacgttgg aaagcagacg ggtcacaccg gcatcagtct
     9901 gcggaatttt ggtggtgctg gtgtaaagca ggttatagag gttggtctga acgtagttct
     9961 gcaaccagtc gagcccgtgg cgctcgtcga agaagtcacc gttcgccatg acaccctgtt
    10021 gcaggatcgc cgtgtcgttg gcgtagtaca cgaatacatt gcagtttttt gcatcaagtg
    10081 ccgatgcctg gctgactgtc agtgtttcat acccgacacc cggctcctgc ttaaacttga
    10141 gcgtaatcgc ggtattactg ccattgaaat taaccgtgaa tgcccggcca aatgcagata
    10201 acgcagcgta tttattaccc gatgaatatt gaataaaact gcgtgaatat ccggcggttt
    10261 tcagttttga tgccaaatca tcgctggatg cagtctgcag gcatttctca tcgcttgtcg
    10321 taatcgccag aatacggctt acagaagagg attcgatcgc cgcagccact ttcagccagt
    10381 ctgcatccgg aatatcttca tcgtctgcaa tccccagccc ataccatgaa gtataatcga
    10441 gcatggcatt cacagcctgc tccagcgtct caggcgtggc ctgttcgctg tctcccttcg
    10501 ttttcaccca acgaccaaca aaaacctcct gaggtttcgg tgattgtgag aaaaacacct
    10561 gcgcagcctt atattctggt gattccacgc caaaatcttt tccaatatct tccgcggcag
    10621 aataacggcg aatgcgctca cttaccggaa tgattgtgga cgggccgaga atgagtaatg
    10681 caccaaaatt tcgccctgat gctgcacgcg gcgacatgat cacatcaaca ttaacaacgt
    10741 ttgatacagg caagccctgt gccatagctt aatctccgaa aaagatgact ggtgcttcca
    10801 ccagcgattt aataccgtac tcgcgcacaa ccttccggcg cagacgcacc gtcatatcgt
    10861 agcggcggac ccattgctga ttaataagtt cagggaagag acctgtgtaa tcgccaagag
    10921 acagccccag cgcattcagt gctgcgttgt tctgcggtac agatataccg tcacgaaacc
    10981 gggacgcata caccatcccc gccggaccat aaaacgaagc catacactca atcgtttcat
    11041 gccgccagag ctgagagcca tcatcggtct gtctggtgaa tgccggactg tcatcacctg
    11101 accatccgat aaccccaaac gcacaccagt tcgtttcaac cggtagcagt ggcggctgct
    11161 ctttctgcca gcgcgggcga accatcccgg cagacagacc ggaaacgtta cgcatccact
    11221 ggcttaacag cctgtcgagc gcttcgtcat aatccggatc gccactggtt ggtattaacc
    11281 atccgcgctc tgtactggtg ttattgctca accggagttc ccccatcaaa cggcatcaac
    11341 tcacaatgcg cctgaacgaa tccggcccca taagctgtat acgggtcgac gaaggtcaca
    11401 cgataatcac ggccctgata cgtcacgata tcggcatcac ggccagtctg tccctgcgtc
    11461 agtcgctcag tcgtcacaat cagaattgca ccgctgatta cctgccctgc ctgcatacgg
    11521 cggttttcca gagagcgatc aacagttacg actccggcaa actgcttttt aacttcactg
    11581 tcgctgccga tcccgtcctc atccaccgtt tgcactcggc gtgttaccca caaattgaag
    11641 tcgcaaaaat cggggtcaaa aagcacatct gttacatcaa gagtcggcat ctttatccct
    11701 cacaacatgg gtaatagctc tgcgatattg cccggtgtca attaatggtt tcgccagatc
    11761 ggttccggga gattcgccag caacacgccg agcaagttcc agtgttgccc ccttgcgccc
    11821 ccgacgagcc cgggcttcaa cggtgctatc agcaagcggt gtaaagccgg taatggtcat
    11881 gtaacgcctg acgccattcg cggccagcgt tccggcgcgg ttaagcgctc tttccgcacc
    11941 cgccgcattt ccatcaagcg cagcctgcgc cgctgctttg agctgcggca ccgtctgttc
    12001 ctctacggat ttaacgccgg ggatcaggtg cgggcgtggg ggtatgtttt gcgctggtga
    12061 gccgtattcg ttgacgtaac cgatcccggc attaccaaac ggaacatcct cacgctcgct
    12121 gtcttcttcc gggatgccca ccagcacatc ctttttggtt agcgaccgga gcgcatccag
    12181 aatggcctga gcgttatcaa ccctcgttgt tacaccactt ttgaaactca tagctggcga
    12241 ccgcccgcac cgaacatcgt gatcagctga taaaattcag cgccatatcg ggtgttattc
    12301 cagaagcctg cgtcagggtt tagcgtcgcg ctggtgtcat agctgacgct taccttgtca
    12361 acggacttgg aggactgaac accattggtt gaaccgcccg ggccgccaac cagcattgcc
    12421 cgactatctg ccgcccatag cgtcatgtag tgagccacga acaacccggc aaagtacgga
    12481 aacaaatctt tgccggtgac gttttcgctc agcagttcat cggccagatt cagacgaaac
    12541 tggatttgcg cttcgggata tttggcaggg tcagcaaact gcgggaagtc gcggcgaaaa
    12601 tcacttaccg ctggcaggct ttgattcttt ggcatttttt acctcgttac gcgcgtctgt
    12661 ggctttgcca acggatactt ccgcgtgcgc acgagtgaac cagtgcgtgg caacgtcttc
    12721 ctccactgca tgacggcctt taacaaactc gcgccgcgaa ccgtcgggaa gcgtgagcac
    12781 aaacggggta tgtacgtgta ttactgcatt attttttgcc atcgggtcat ccttaatggc
    12841 cccgccaggg gggccatgtg gctgttaaat gccatcaacg tacgaaatgg tttctttgta
    12901 cactggctca accgcaccca gcttgccgta gtaggtcgca atctgataca gaccgcgata
    12961 ctggatagga acgctctgca acggcaccag cggatagcgg acgtattttt tatcgttggt
    13021 gtacgcaacc atgcgatcct tattccccac accacggcct ttcagccatt taaccgcgcg
    13081 gatattcagc ggaacaccgt tctggtgata gctgatggtg ttggtctgaa ggtacgtcaa
    13141 cagggactgg ttacccgcag atgaaacgat gatgctggac aacagagcaa actgctctgg
    13201 cgggatcagc aaatcacgcg gaaccacaga gtaaccggaa gcggcccacg catcagacag
    13261 cacctggtta atgcttgcgc ggatttcgtc cggtgttgag attgcccacg ttttggcagc
    13321 gttgttgaca ggcacgccgt ccagggtaac aaggcctttc aggtttaatg cggaatcgcc
    13381 aacatacacc tgctcgtcgt tgtccatctg ccatttgagc tgcatcccgt cgtacttctg
    13441 ggtgtcaatc gggcgaccta cctgctgagc tgctgccagc tctacaacgg tccagcccag
    13501 ttccatgccc cagagattca gcgggttacc ggatttgccg atatccacgt ttacgccagc
    13561 aatagcggtt gagtctttgc ctacccagtt tttgccattc ggatttgcac cagtacccgc
    13621 agcggcgaag ctggtattcg tccagctgga aatgtcatct gcgatagaga catcttcacg
    13681 caactgaata tcgcgggtcc aggtgtaccc cgccagtggc aggttcagag tctggtcgag
    13741 tcgctccagc tctccgatga gaaaggcacc agagctgtca acggttgcct gatcaaaagt
    13801 aatcattcgt ctgttcctta aatcttccag gaaatttctg cattgccgtt agcatcaccg
    13861 gcacctgtga attcagcgtt ggtcagcacc acatttttgc cactgactga cgtggacatg
    13921 aatccaccca gcggcacttt gatggattca tcagtggaga cgacaacgta taccgggtcg
    13981 ccttttttga tggtgctggc atcaaaatca gaaccgagat taacggtcac gtagccacgc
    14041 ttcatggcgt cgcccgggaa gttcttgcct gttcccacct ggcgaaccat gtccggctgc
    14101 gaagtggtcg gataagggcg cacgtagatc cccttcacct tgtctgcggt atcgccatct
    14161 gccagcggca cgaaaaaacc gtcagcatcg tatttaccag ccagcccata ggcagcaaag
    14221 gcgttatcgg atttaaggac caccggttcg acggttaagt cctgcgggcg agagatagcc
    14281 ccggcaatgc caacaggcat ccggtacaga tatgcagtca ttggattatc ctttgcggtt
    14341 agaccagaat tcagcgtttt gtttgttcag ggaagcgatg ctggtcatgc ccatgtttgg
    14401 acgttgtgca tcgcccgtgg tgctgcgggt gtttcgccct ttggcaatct cagacacagc
    14461 gttaaacgcc atatcgaccg attgcttagg caatttgcgg atatccgcat caccgacaac
    14521 ctggcgaacc agcgttttgt cagcagaagc cagaacctcg cgtttgaac
//
LOCUS       DAJVRL010000095        14528 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVRL010000095 DAJVRL010000000
VERSION     DAJVRL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.095"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(390..635)
                     /locus_tag="OQN02_003070"
     CDS             complement(390..635)
                     /locus_tag="OQN02_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643781.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1280..1355)
                     /locus_tag="OQN02_003071"
     tRNA            complement(1280..1355)
                     /locus_tag="OQN02_003071"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1320..1322),aa:Phe,seq:gaa)
     gene            complement(1461..2168)
                     /locus_tag="OQN02_003072"
     CDS             complement(1461..2168)
                     /locus_tag="OQN02_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1643782.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2566..4701
                     /gene="speC"
                     /locus_tag="OQN02_003073"
     CDS             2566..4701
                     /gene="speC"
                     /locus_tag="OQN02_003073"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1643783.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4750..6006)
                     /gene="nupG"
                     /locus_tag="OQN02_003074"
     CDS             complement(4750..6006)
                     /gene="nupG"
                     /locus_tag="OQN02_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1643784.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6208..7287)
                     /gene="mltC"
                     /locus_tag="OQN02_003075"
     CDS             complement(6208..7287)
                     /gene="mltC"
                     /locus_tag="OQN02_003075"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1643785.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7352..7627)
                     /locus_tag="OQN02_003076"
     CDS             complement(7352..7627)
                     /locus_tag="OQN02_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1643786.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7655..8707)
                     /gene="mutY"
                     /locus_tag="OQN02_003077"
     CDS             complement(7655..8707)
                     /gene="mutY"
                     /locus_tag="OQN02_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1643787.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8868..9587
                     /gene="trmB"
                     /locus_tag="OQN02_003078"
     CDS             8868..9587
                     /gene="trmB"
                     /locus_tag="OQN02_003078"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1643788.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9587..9913
                     /locus_tag="OQN02_003079"
     CDS             9587..9913
                     /locus_tag="OQN02_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1643789.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10097..10816
                     /locus_tag="OQN02_003080"
     CDS             10097..10816
                     /locus_tag="OQN02_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1643790.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10992..12038
                     /gene="ansB"
                     /locus_tag="OQN02_003081"
     CDS             10992..12038
                     /gene="ansB"
                     /locus_tag="OQN02_003081"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1643791.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12155..13116
                     /locus_tag="OQN02_003082"
                     /pseudo
     CDS             12155..13116
                     /locus_tag="OQN02_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13217..>14528)
                     /locus_tag="OQN02_003083"
     CDS             complement(13217..>14528)
                     /locus_tag="OQN02_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1643792.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKL
                     ILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLI
                     VGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCS
                     ISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3807 a         3484 c         3735 g         3502 t
ORIGIN      
        1 gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat caggctgagg
       61 agtccccaga aaagaagaca ggcatagagt tttgtgatct actgattgcg ctaacaaatt
      121 caatgagatc acctctatgc ctgcccgtta agtatgtcag gcttttttcc gtaatgctct
      181 aacttccacg cattggtttc tgacatcaaa taaggctatg actttctctc ccttttaatc
      241 tcattctgat ctaaaaccat atcttaaaaa aatgcatggg tattttaatt attttgatgt
      301 tgatctaacg cactcaagga ccattttatt accgattatc cattctattt aactgttgcg
      361 atataaatgt ttgttttagg tgctttaatt cagtacaagt catcaaaccg taccagaccc
      421 tgactgtcag tgacaccgac aggtgggggc ggcggtgtgg ggtcaaactg agtgtgtctg
      481 cctctggccc acagagccag ctctgccgcc attgcccgtg gatgatactc cagctgcacg
      541 ttgagaccgg aaactgggtt gttgttctca tccacgaact gcagcgccag ccagtgcatc
      601 cgctgcttac cgcaggcgac ttctttgtac ttcattttgt gactcaggca ggtgactgtc
      661 gtgttgcctg ttgcgtccat tttgcggtta aaccctttta attcaatttg ttaaattgct
      721 ttccaccttt tgcggggcgc gacttaacca aaatattcac tgttatcata atatgcaatg
      781 ggctggaggt tgcaggtcat ttagggattt tctgtaagcc ccgttttgca gaacgttcta
      841 tctccgattg agatcctgtc tctcggtatt accggtagtc tggccataat cccctatgga
      901 tcatattgat atagtaggca taacacccaa aagttgacta aagctaaatc aatcagagtg
      961 aaaaatgttc ggtttgatgt agggggggac tgaggagggg ccaacgcagg tggtatgcgt
     1021 tttacttcag accggacaaa gaccggatgt atccgtgcag taatgaatcc gttagccagg
     1081 tgctcatgtg gaaatagtgt ctattgggga tttgaagttt gacagaaaag gccacagtga
     1141 ccagaagggg gctatttaga agaaatgttc aggctggatg gaagaggtaa agcagtgaca
     1201 tcagcattca aagtgaccgg atatctacca gaaccccaga aagcaaaaaa ccagcccgta
     1261 ggctggttct tctaaatagt ggtgcccgga ctcggaatcg aaccaaggac acggggattt
     1321 tcaatcccct gctctaccga ctgagctatc cgggcaacgg ggcgcattaa acctgattcg
     1381 cctcgtctcg tcaatcaaat ttcttcaatt tactgcagac tgcacaatct atcatcactt
     1441 tgccgctatt gcacgcattg tcaggcaaac caggcggtcc aggcggcgga aagcggcatt
     1501 gccagaagga gtgctggaag catgttgacc accgggaaca ttttaatgcc acagatgcgt
     1561 aatccggttg ccagcagcaa taaaccgcct acggcgctga agtctgccat catcgacggt
     1621 gtgatcagcg gtaatatcag cgcggcagcc cacgccagcg ttaactggat gatcagtaat
     1681 gggatactaa ttaccgacac cgcaataccc agcgagcagg cgaagatcat cgccgtaaag
     1741 aaatcaagaa atgacttggc gattaaaata ctcggatcgc cggtcatccc ttcgttcatc
     1801 gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa taatcgcgac ataattctga
     1861 ataaaagatt catgcgctgg cttcttacgt gagtgacgaa acagattttg tgctttggcg
     1921 actgctgtat tgacaccttt ctccagcaaa caaatttcgc caattagagc gccaagtagc
     1981 gtagctaaaa ccatcgctgg aaggttggcg catttcacaa ccagtaaaat accaatcccc
     2041 agcgatgcca gaccaaaaat agatgtcatg gagacgcgga tacgttccgg taagcgttgg
     2101 ctgagcagtg cgccgagaac gccacccagt aagactgcac tagcgttgat aaaagggccg
     2161 atgaccacaa gagttcctgt tagctgtatt gtttgatttt cttattatga actttttgcc
     2221 ttgttgatgg cttgtctggt cgcaaagtcc tggcttgcac gccttagcga aaggtgccat
     2281 gattgcgcga attttctcct ctctgtacgg agtttgcccg atgcacgcca tctccttaca
     2341 ttctctcgct tatcgccgtt tcgcgcgaaa cgtttccctt tctatgttac tgctcatgcg
     2401 gtgaagttaa cgcacgctca ctgcaggaca acagtaaaat cagagcgttt ctgcttttac
     2461 tgatgtctgg cggtcggagc tggtgaccag tttgacccat atctcatggg gcagggtttt
     2521 ccaccttgtc cggtattctt acttccccga aacgggtttg cgcttatgaa atcaatgaat
     2581 attgccgcca gtagtgaagt ggtatcccga ctttcttctc atcgccgcgt ggtggcgttg
     2641 ggagatactg attttacgga cgtcgcggca gtcgtcatta ccgctgcgga tagtcgcagt
     2701 ggcattcttg cgttgcttaa gcgcaccggt tttcatctac cggtgttttt gtattccgaa
     2761 catgctgttg aattacctgc gggcgttacg gcggtaatca acggcaacga gcagcagtgg
     2821 ctggagctgg aatccgcagc ctgtcagtat gaagagaatt tgctgccacc gttttatgac
     2881 acgctgacgc agtacgttga gatgggcaac agcacctttg cttgccctgg acatcaacat
     2941 ggtgcgttct ttaaaaagca tcctgccgga cgccattttt acgatttctt tggtgagaac
     3001 gtctttcgcg ccgatatgtg taacgctgac gtaaaattgg gcgatctgct tattcatgaa
     3061 ggatcggcga aagatgcgca gaaattcgca gccaaagtct ttcatgccga taaaacctat
     3121 tttgtgctga acggcacatc ggcagcgaat aaagtggtga cgaatgcgct gttaacgcgt
     3181 ggcgatctgg tgctcttcga ccgtaacaac cataagtcga atcatcacgg cgcgctgatt
     3241 caggcgggga cgacgccggt ctatctggaa gcttcacgta acccgtttgg tttcattggc
     3301 ggtattgatg cgcactgttt taatgaagag tatctgcgcc agcaaattcg cgacgttgcg
     3361 ccagaaaaag ccgacctgcc gcgcccgttt cgcctgacga ttattcagct gggaacctat
     3421 gacggcactg tctataacgc ccgtcaggtg atcgatacca ttgggcatct gtgtgattac
     3481 attctgtttg attccgcgtg ggtcggttac gaacagttta tcccgatgat ggcggatagc
     3541 tcgccgctgc tgttagaact taacgaaaac gatccgggga tttttgtgac ccagttggtg
     3601 cacaaacagc aggctggatt ctcacagacg tcgcagatcc ataaaaaaga taaccatatc
     3661 cgcggacagg cgcgtttttg cccgcataag cggttgaata acgcctttat gctccatgct
     3721 tctaccagcc ctttctatcc gctgtttgct gcactggatg ttaacgccaa aattcatgaa
     3781 ggggagagtg ggcgtcggct gtgggctgag tgcgttgagt tgggtattga gtcgcgcaag
     3841 gctattcttg cgcgctgtaa gctgttccgc ccgtttatcc cgcccgttgt tgatggcaaa
     3901 ttgtggcagg attatccgac atcagtgtta gccagcgacc gccgtttttt cagttttgag
     3961 ccgggggcga agtggcacgg ctttgaagga tatgccgcgg atcagtattt tgttgatccg
     4021 tgcaagctgt tactcactac accaggtatc aatgccgaaa ccggcgaata tagcgacttt
     4081 ggcgttccgg cgacgattct ggcgcactat ctgcgtgaga acggcattgt gccggagaag
     4141 tgcgatctca actccattct gttcttatta actccggcgg aaagccacga gaagctggca
     4201 caactggtgg cgatgctggc gcaatttgaa cagcatattg aggatgactc gtcgctggct
     4261 gaggtgttgc cgagcgttta taacaaatat ccggtgcgct atcgcgacta caccctgcgc
     4321 cagttgtgtc aggagatgca cgatctgtat gtcagtttcg acgtcaaaga cctacaaaaa
     4381 gcgatgttcc gccagcagag tttcccgtca gtggtgatga acccccagga tgcgcatagc
     4441 gcttatattc gcggtgaagt ggagttggtg cggattcgtg atgccgaagg gcgaattgcg
     4501 gcagaagggg cgttgcctta tccacctggc gtgctttgcg tggtacccgg ggaagtctgg
     4561 ggtggggcgg ttcaacgtta tttccttgcg ctggaagaag gcgtgaattt gttgccggga
     4621 ttttcgccgg agttgcaagg tgtctatagc gaaaccgatg cggatggcat gaaacgattg
     4681 tacggttatg tgttgaagta agaataaaaa aacgggtcac cttctggcga cccgtttttc
     4741 tttgcgtaat tagtggctaa ccgtctgtgt gcctgtcggg acacgaacgt gtttatattt
     4801 gaacatcgcc atgaacgcga aggccagaac cacggaataa ccagcgaaaa tcaaccatac
     4861 ggtctgccag tcggtaatgc cgttttgggt gtacatctca acaactttac cgctcacgat
     4921 gccgccgagg atacagccga agccgttagt catcatcagg aacatccctt gtgcactggc
     4981 gcgaattgcc gggctaactt ctttttcgac aaacaccgaa ccagagatgt tgaagaagtc
     5041 gaatgcgcaa ccgtaaacga tcatcgacag taccagcagt acagtaccga acggagtcgg
     5101 gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc acaatactga tcatcattac
     5161 gttcttaata ccgtagcggc ttaagaagaa cgggatggtc agaatgaaca gggtttcaga
     5221 gatctgcgaa atcgacatga tgattgacgc atgctgcacg atgaagctgc tggcaaacat
     5281 cggatctttg tcgaagctgt gcaggaaggt attaccgaac atgttggtaa tctgcagttc
     5341 cgcgcccagc agcattgaga agatgaagaa gattgccata cgcttgtttt taaacagcgc
     5401 gaatgcatcg aggcccagca gggttgtcca gctctgattc gcttgctgtt tagcaaccgg
     5461 aatatgcggc agagtcaggg taaacagaac cagaatggcg gaaagtgctg cgccaatata
     5521 cagctgcatg tggcttaatt cgaagccaga caggctcacc acccacattg ccatgataaa
     5581 gccgatggtg ccccagatac ggattggcgg gaagtcagta acgatatcca tcccggcatt
     5641 ttgcaggcga tagtaagaga tggtgttgat taacccaagc gttggcatat aagcaaacga
     5701 gttaatcaat atcacaagga acatcgcttc cggcgtcgtg acctgtgccg ccatgaacag
     5761 cgtgatagcg ccaatggtgt ggcaaatggc atatacccat ttcgcactta accatttgtc
     5821 ggccacaatc cccagcagcg caggcataaa taccgcggcg atacccagtg agctataaac
     5881 tgcgccaata gaagcaccgt caaacttcag ggtaacaaac atataggagc cgagggtcgt
     5941 cagccaactt ccccacagac agaactgcag aaaagagagg attttcagct gcagcttaag
     6001 attcatgtta atttcctcac atcgtgatgc ggatgagtgt ttgaaaaagc acatttgacc
     6061 gtgcgagatg atgggattct atcaacttcg cggactggtt ttttgttacc tgtggcaaat
     6121 aattgcaaac attcccaatt tgcaaaacga aattgtgatg tggataacat ttttgcccct
     6181 gagcatcgtc aggggcggtt aatggaatta tcggcggcgg taggattttt gcgcggtatt
     6241 cactttataa agataacggc gagattccgc agagggatgg cgggtcgtca gcgtctgata
     6301 aacatcgccc ggcgtcatgg tgttaataat attggcagcc tgaatcttat cattcgaaaa
     6361 gactcgcagc acgctgcctg cgccgccgtt ataggcggtg atgacggcat aacgccgcga
     6421 tgttgggtta tcaattccgc cgagataaac attgttcagc atcgccagat acgcggtgcc
     6481 ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg ctcggcgtgc cggatttccc
     6541 ctgcgagcgg aacacatctt tcccggcagt attttgtacc acctgcatta atcccagcgc
     6601 atcggaacgg ctgaccgcat acgggttaaa ggaagattca gtctgcataa ttgccagaat
     6661 cagcgactca tcaacgccat atttccgtga cgcctggcgg accatgccga gatatttgtg
     6721 cgcacgttta tcaaggtggt tcggcaccat gttaatggtg acgctgtaga tgatacgtag
     6781 cccgttgctg cggcttttca gacggttttt cagcagataa tccgcgaagt tgcttgcgcg
     6841 accttcccag cgaatcggct gcccggtgtt gtccaccacc tgaccgtaaa ggaaaggttc
     6901 tttcgaaatt gtaatatcat caacgtcgga atagagatcg accgaactcg gatcgtcacc
     6961 catcagcaac gttttgataa ttgcccggcg caaatgcgcg gcaggttctg tcccggcgat
     7021 ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg ctgcgggtct gatattgatc
     7081 ggtgtatttc acgtagtcct taggaccggc gatcaccacc tctttgaagc cccagatgtt
     7141 ctcaatattg tgggcaaatt gccccatcag aatatcaaaa ccgttggtat ctttgaccca
     7201 ggcttcgtta taggtatcgc cttttttggt cgtcgaacag gagatgagca acggcgcaat
     7261 caaagccagc gcgagatatt ttttcatcat tccgggtgcg tgttgtgttt gtttttatgc
     7321 aagctgccgg aggcgtgctc cggcactgtt tttatttttt atcttccggc gtatagccct
     7381 cgatatgcac ctctttaccc tcgaacagga agttgaccat ctcctgctcc agcagcttgc
     7441 ggtgctcggc attcatcatg ttgagtttct tttcattaat cagcatggtt tgtttgtgct
     7501 gccactgcgc ccaggcttct ttggagatct cgttatagat gcgttttccc agctcgccgg
     7561 ggtacagctg aaaatcctga ccttctgctt cacgttgcag gaaagtacaa aaaatcgttc
     7621 tgctcataaa tcatcctctt tatcgactca cgcgctaaac cggcgcgcca gtgcgtaact
     7681 gctgtaataa acgctccacg ggagccgcca ggccaactga cggcggttgc gctaagttat
     7741 accagagcgc attgccttca tccatgcagc cggtgaatga cgacacggga agccacatag
     7801 gcacaatatc taagtggaaa tggctgaagg tatggcgaaa cgcggtcagt tgcgtcaggt
     7861 tatcggcagc aatctgccgt tgtgccagcc aatgccgcaa actttcttcg tcggcaaact
     7921 gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg acgctgcgcc agcaatactt
     7981 catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc cggcagcgtc tgtttcggtt
     8041 ttttgcccgg ataaagcgac cagctattgt tggtggcggc aatacatccg ttttgtagcg
     8101 gacagagcga acatttcggc ttcgaacgcg tgcaaatcat cgcgcccaaa tccatcatcg
     8161 cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg ctcgctcaaa ctccatagtt
     8221 tattctcgac ctcttttttc ccaggccagc cgcttacagc atagcagcgc gccagcacgc
     8281 gtttgacgtt accgtcgaga atcggaaagt gcttacccag agaaagcgag agaatcgcgc
     8341 ctgcggtgga acgcccgacg cccggtaacg ccgcgacttc ttcaaaggtt tccgggaatt
     8401 taccaccgtg taaggtcgcc acttgttgtg ccgctttatg caggttgcgc gcgcgggcgt
     8461 aatagccaag cccggtccac aagtggagaa cttcgtcgag cggcgcattg gcgagatcgg
     8521 tcaccgtcgg gaagtgcgcc ataaagcgtt caaaataggg gataacggtc gcaacctgag
     8581 tttgttgcaa catcacttct gagagccata ctttgtaggg cgtcttgtca atttgccagg
     8641 gcagcgtttt ccgcccgtat ttatcgtacc agtccagaac ctgggctgaa aattgcgacg
     8701 cttgcatggt caccgacttc actgttgttg ggggcaagat tgcagcacag cgacggcagg
     8761 gtgtaaaccg gaactttccg cagccacggc cttatcatgc ttgcatccgg caactaactt
     8821 tggataatgc ccgttttcag aacactttca caagcgacta aatctttatg aaaaacgacg
     8881 tcatttcacc ggaatttgat gaaaacggcc gcccactgcg ccgtatccgt agttttgtgc
     8941 gccgccaggg gcgactgacc aaaggccagg aacatgcgct ggaaaactac tggccggtga
     9001 tgggcgttga gttcagtgaa gatatgctgg atttccccgc gctttttggc cgtgaagcgc
     9061 cggtgacgct tgagattggt tttggcatgg gggcgtcgct ggtggcaatg gctaaagatc
     9121 gccccgagca ggacttcctc ggcattgaag tgcattcacc gggcgttggt gcgtgcctgg
     9181 cttctgcgca tgaagaaggt ttaagcaacc tgcgcgtgat gtgtcacgat gcggttgaag
     9241 tgctgcataa aatgattcct gacaattcat tgcgcatggt gcagctcttt ttccctgacc
     9301 cgtggcacaa agcgcgccat aataaacgcc gtatcgttca ggtgccgttt gccgaactgg
     9361 taaaaagcaa actgcagctg gggggcgtat tccatatggc gaccgactgg gaaccttatg
     9421 cggaacatat gcttgaagtg atgtcttcta ttgacggtta taaaaacctg tcagagagca
     9481 atgattacgt accgcgtccg gcatcacgtc cggtgacgaa atttgaacaa cgtggtcatc
     9541 gtcttggtca cggagtatgg gacttaatgt tcgagagggt gaaataatgg caaagaaccg
     9601 tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc caggaattag gattttcggt
     9661 ggcatggcga ttcccggaag gtacatcgga agaacagatt gataaaatcg ttgatgactt
     9721 tattaacgag gttatcgaac cgaacaaact ggcctttgac ggcagcggtt atctggcctg
     9781 ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc gaagaacatc aggcgattgt
     9841 gcgtaagtgg ctggaagagt gcaaactgga tgaggtacgc accagcgaac ttttcgacgt
     9901 ttggtgggac taagaaagca tacgggcgat gacaaatgca aaactgcctg atgcgctacg
     9961 cttatcaggc ctacagagat gcacgatcga gtaggcggga taaggcgttt acgccgcatc
    10021 cggcatggaa aacgcgtact ttgttatcaa tctggggcca gcatatgctg gcctgatttg
    10081 ttcttgaggg aagactatga tgcgcaaaat gctgctggcg gcagcacttt cagtgacggc
    10141 aatgaccgct cacgccgact accagtgcag cgtcacgccg cgtgacgatg tgattgtcag
    10201 cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc cagacggcaa
    10261 cgtgatgtat aacggtaagc aatattccct gaatgccgcc cagcgcgagc aggcgaagga
    10321 ttatcaggct gaactacgta gcacgctgcc gtggattgat gaaggcgcga aaagccgcgt
    10381 cgagaaagcc cgtattgcgc tggataaaat tatcgttcag gagatgggcg acagcagcaa
    10441 aatgcgcagc cgtctgacca aacttgatgc gcagcttaaa gagcagatga atcgcattat
    10501 cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc attgatcagg ttcgcgccga
    10561 aggccagcaa ctagtgaatc aggcaatggg cggaatttta caggacagca ttaatgaaat
    10621 gggcgcgaaa gcggtgctga aaagcggcgg taacccatta cagaatgtgc tgggaagctt
    10681 gggcggtctg caatcctcaa tccaaaccga gtggaaaaag caggaaaaag atttccagca
    10741 gtttggcaaa gatgtttgta gccgcgttgt gactctggaa gatagccgca aagccctggt
    10801 cgggaattta aaataatcct ctattttaag acggcataat acttttttat gccgtttaat
    10861 tcttcgtttt gttacctgcc tctaactttg tagatctcca aaatatattc acgttgtaaa
    10921 ttgtttaacg tcaaatttcc catgcaaagc taagggataa tgcgtagcgt ccacgtaact
    10981 ggaggaatga aatggagttt ttcaaaaaga cggcacttgc cgcactggtt atgggtttta
    11041 gtggtgcagc attggcatta cccaatatca ccattttagc aaccggcggg accattgccg
    11101 gtggtggtga ctccgcaacc aaatctaact acacagcggg taaagttggc gtagaaaatc
    11161 tggttaatgc ggtgccgcaa ctaaaagaca ttgcgaacgt taaaggcgag caggtagtga
    11221 atatcggctc ccaggacatg aacgataatg tctggctgac actggcgaaa aaaattaacg
    11281 ccgactgcga taaaaccgac ggcttcgtca ttacccacgg taccgacacg atggaagaaa
    11341 ctgcttactt cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg gtcggcgcaa
    11401 tgcgcccgtc cacgtccatg agcgcagacg gtccattcaa cctgtataac gcggtagtga
    11461 ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt ggtgatgaac gacaccgtac
    11521 tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt agcgaccttc aagtctgtta
    11581 actacggtcc tctgggatac attcacaacg gtaagattga ttaccaacgt accccggcac
    11641 gtaagcacac cagcgatacg ccattcgatg tctctaagct gaatgagctg ccgaaagtcg
    11701 gcatcgttta taactacgct aacgcatccg atcttccggc taaagcactg gtagatgcgg
    11761 gctatgatgg catcgttagc gctggtgtgg gtaatggtaa cctgtataaa tccgtgttcg
    11821 acaccctggc aaccgccgcg aaaaacggca ctgcagtagt gcgttcttcc cgcgtaccga
    11881 cgggtgctac cactcaggat gctgaagtgg atgatgcgaa atacggcttc gtcgcctctg
    11941 gcacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcagct ggctctgacg caaaccaaag
    12001 atccgcagca gatccagcag attttcaatc agtactaatc acctcgtccc ggcatcgtgc
    12061 cggggctttt tcacttcaga cttacgtcca ttgccaattt tcattaccct aatgataatc
    12121 accggaataa attattccgc gcgagggttt tcgggtgaaa aagcaatgga ttgttggcac
    12181 ggcgctgctt atgttgatga ctggtaatgc ctgggcagat ggcgaaccgc caactgaaaa
    12241 tatcttaaaa gatcaattca aaaagcagta tcacggcatt ctcaagcttg atgccatcag
    12301 cttaaaaaat cttgatgcta agggtaatca ggccacttgg tcagcggaag gcgatgtctc
    12361 ttccagtgac gatctctata cctgggtcgg tcagttggca gattacgaac tgctcgaaca
    12421 gacctggacg aaagataaac cggtaaaatt ctcggcgatg ttaaccagta aaggaacgcc
    12481 cgcgtctggc tggtcggtga acttttactc ttttcaggcg gcagccagcg atcgtgggcg
    12541 ggtggtcgac gatatcaaaa cgaataataa atatctgatc gtgaatagcg aagatttcaa
    12601 ttatcgcttt agtcagcttg aatctgcgtt gaatacccag aagaactcga ttcctgcgct
    12661 ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg gcgcagaaag cagcggatgc
    12721 atattgggga aaagatgcaa acggtaagca aatgacccgc gaagaagcct ttaaaaaaat
    12781 tcaccaacag cgtgatgagt ttaataaaca gaacgatagc gaggcgttcg cggttaaata
    12841 tgacaaagag gtttatcaac cggcgattgc ggcatgtcat aaacagagtg aagagtgtta
    12901 tgaagtgccg attcagcaga aacgagattt cgatatcaac gaacaacggc gacagacttt
    12961 tctgcaatca caaaaactca gccgtaaatt gcaggatgac tgggtaacgc tcgaaaaggg
    13021 caatatccgt taacgatgaa agtttcagaa ataaacagta aaaaagtcgc gattttgatg
    13081 aaaattgacg atatcaacca agctaatgaa cgctagaaaa aagataccga acaactgcgg
    13141 cgcaatgggg tgattaagta actcatgtta agtgcctttg ccggataata taaaaagtta
    13201 tccggcaaat tattttttac attagcccta gtagctgtgg aatgaacaag gatatttgcg
    13261 gaatgtaagt gatcatcatc aacgctacta gcaatgcagc ataaaatagc agcaatggtt
    13321 tgattaaatg ttgaatcttc acacccgaga tagaacaacc aacaaatagc gcactaccca
    13381 ccggcggtgt taacagccca atacaaaggt tggccaccat cataataccg aaatgtactg
    13441 ggtccattcc cagctcttgt gcaatcggca agaagatcgg tgtaaagatt agtacagccg
    13501 gtgtcatgtc cataaagatg ccgacaatta ataagacgat attaatcagc agcaatatga
    13561 tcagtgggtt atcagaaatc cccatcaatg cgtcgctgat catataagga atatcagcat
    13621 tagtcattgc ccaggacatg cccacggaaa aaccgatcaa cagtaataca atcgaagtgg
    13681 taacaaccga ctccagaatc agtttgggta aatggcgcca ttttacttcc cggtaaatca
    13741 gcaccgagag aataaatgtg tagacaacgg caattgctga cgcttcagtt gcggtaaaaa
    13801 tgccgcccag aatgccgccc agaatgccgc ccatcacaat gaacaccaga agcaaacttg
    13861 gtaaggcatc cagaaacgct ttacaggcct gagcaaaggt tgctctttct gaaagcgggt
    13921 agccacggcg tttagcaata atgccgcaga ctatcattac cgcaagaccc attaagatcc
    13981 ctggcaaata tcccgccata aacagcgatg ctacagaaac gccgccagct gtcagggaaa
    14041 acacaattaa cacgtttgag ggcggtatca gtaatccagt aatacaagat gagacgttta
    14101 cagcggtaga aaatgccgga tcataacctt ctttggtctg aatgggattc agtgtgccac
    14161 caacggcagc agctgccgca acggctgaac cagaaatgga accaaacatc atattcgcca
    14221 ggacgttcac atgaccaagc gaaccaggaa cgcggccaac cattacctgg gcgaggttaa
    14281 tcaaacgtat cgcaataccg ccgctgttca tcagtgttcc ggcaaagatg aaaaagggga
    14341 ttgccaacag ggcaaagtta tccagtccat tagccagtcg ttgcgaaaca gtgattgccg
    14401 cgatatccca gggaaacatc aaaagcattg aagctacagt ggcaataccg attgagaacg
    14461 aaatgggcac accaataaag accagcacaa aaaaacagcc aaacagtgta atcgcgatat
    14521 aactatcc
//
LOCUS       DAJVRL010000096        14515 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVRL010000096 DAJVRL010000000
VERSION     DAJVRL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.096"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(113..379)
                     /locus_tag="OQN02_003084"
     CDS             complement(113..379)
                     /locus_tag="OQN02_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1643793.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OQN02_003085"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OQN02_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1643794.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OQN02_003086"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OQN02_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OQN02_003087"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OQN02_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1643795.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OQN02_003088"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OQN02_003088"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1643796.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OQN02_003089"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OQN02_003089"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1643797.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OQN02_003090"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OQN02_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1643798.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OQN02_003091"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OQN02_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OQN02_003092"
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OQN02_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS1643799.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12641..14095)
                     /locus_tag="OQN02_003093"
     CDS             complement(12641..14095)
                     /locus_tag="OQN02_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCS1643800.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14515)
                     /locus_tag="OQN02_003094"
     CDS             complement(14092..>14515)
                     /locus_tag="OQN02_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1643801.1"
                     /translation="FSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVSLVPVSE
                     LWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATG
                     NFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3873 a         3663 c         3429 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caagatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgctg
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgcac gaaccacggc agcctgacgg ctgcaaattg aaaac
//
LOCUS       DAJVRL010000097        14044 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVRL010000097 DAJVRL010000000
VERSION     DAJVRL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.097"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(113..835)
                     /locus_tag="OQN02_003095"
     CDS             complement(113..835)
                     /locus_tag="OQN02_003095"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1643802.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(837..2008)
                     /locus_tag="OQN02_003096"
                     /pseudo
     CDS             complement(837..2008)
                     /locus_tag="OQN02_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2035..3651)
                     /gene="bglH"
                     /locus_tag="OQN02_003097"
     CDS             complement(2035..3651)
                     /gene="bglH"
                     /locus_tag="OQN02_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCS1643803.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(3737..5131)
                     /gene="bglB"
                     /locus_tag="OQN02_003098"
     CDS             complement(3737..5131)
                     /gene="bglB"
                     /locus_tag="OQN02_003098"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCS1643804.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5150..7027)
                     /gene="bglF"
                     /locus_tag="OQN02_003099"
     CDS             complement(5150..7027)
                     /gene="bglF"
                     /locus_tag="OQN02_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS1643805.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7161..7982)
                     /gene="bglG"
                     /locus_tag="OQN02_003100"
     CDS             complement(7161..7982)
                     /gene="bglG"
                     /locus_tag="OQN02_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS1643806.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8269..8994)
                     /gene="phoU"
                     /locus_tag="OQN02_003101"
     CDS             complement(8269..8994)
                     /gene="phoU"
                     /locus_tag="OQN02_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1643807.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9009..9782)
                     /gene="pstB"
                     /locus_tag="OQN02_003102"
     CDS             complement(9009..9782)
                     /gene="pstB"
                     /locus_tag="OQN02_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1643808.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(9874..10764)
                     /gene="pstA"
                     /locus_tag="OQN02_003103"
     CDS             complement(9874..10764)
                     /gene="pstA"
                     /locus_tag="OQN02_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1643809.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(10764..11723)
                     /gene="pstC"
                     /locus_tag="OQN02_003104"
     CDS             complement(10764..11723)
                     /gene="pstC"
                     /locus_tag="OQN02_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1643810.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(11810..12850)
                     /gene="pstS"
                     /locus_tag="OQN02_003105"
     CDS             complement(11810..12850)
                     /gene="pstS"
                     /locus_tag="OQN02_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1643811.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13098..>14044)
                     /locus_tag="OQN02_003106"
     CDS             complement(13098..>14044)
                     /locus_tag="OQN02_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1643812.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3616 a         3629 c         3178 g         3621 t
ORIGIN      
        1 tccgctgtct ccactgcggc aagctgagac tgtggggtga ttatttcgta gttatttaaa
       61 taatgagaac aggccggagc gtaattcata catccggcct tatttcttca gcttaaccga
      121 acgctaattc tgcggcagcg gctttatcag cgattaccat cagcgaagga tgcagctgca
      181 acacggaagc tggaacatct tcggtaaccg gaccttgcag cacatttttc agtgcctgcg
      241 ctttacctgc gccactgaca attatcagca agtttttcgc cgccatgatg cttttcggcc
      301 ccatcgtcac gtagctgtca ggcactaacg aaaagtcgcc gcccaactca ccatgcgcca
      361 caatatcaac catctctccc tggatcggga actccacggt ttgctcatgg aaatgggtcg
      421 tattcggcaa attgccacaa aaatgaccat ctgcacctaa tcccagcacc accaaatcca
      481 ggccgccttc acgcgccagt ttctgatcat gctcgcggta gttatcaata gtgagcttct
      541 ggatattctc ttctttgatc cccgcagggg tgaaaaacag attacgcaga ttggtaatcg
      601 ttacgccttc tccctctttg ccgcgaaagg gaatttcatc gaaattatag aaatagcagt
      661 tatcgtacca aggcttacct ttaaccaggg tggtgaggta ttcatacatg cctttgggcg
      721 tgctaccggc ggtaattgcc aggttaacac gacgcgtctt cgacatataa ccaagcagat
      781 ggtgtgccgc gacacggctc atttcctggt aatcttcggt aatgattaat ttcatcttaa
      841 tgccttattt taaatttctg tacgaaggct gcataggcag gccgccagac atccatttca
      901 tggttcaggc cgggatattc ctggtaatca aacttaattt ttttctgctc aagctcagtt
      961 ttcagcccgg cgatatcctt gccggttacg acatctttat caccaacgac cacagtaaaa
     1021 ttacgtagtt gctggttaat agctgccgga tcgttcagcc gggccgcgac accttcatcc
     1081 ggtacggttg tcgtggtaac accactgaat gtggccagcc aaagctttcc agatgattca
     1141 ttccggaaac cagcgcctgg tacccgcctt gtgaaagccc tgccagcgcg cgaccatcgg
     1201 catctttacg gacactaaaa cgcttgctaa tcagcgggat aatatcgttc atcagttcgc
     1261 gatctgccgc tttagcattc agcggataaa agactttacg tctttcctga ggcacgaaat
     1321 cttcgggaat aatgcccttc gcatcggttt cggtatccgg gatcaccacc agcatcggtt
     1381 taattttccc ttcagccagc aggttatcca tgatttgcgg gatacgcccc tgatcgatag
     1441 cggaacgtcc ggtatcacca aaaccgtgat agaaatagag cactggcaaa ggctcgccca
     1501 tgccggtgta tcctggcggg gtccagacat acatctgacg ttcagattgc aatgcgttgg
     1561 agtggtaagt tatggcgatc aaatcaccat gcgcaacaga acgcgtatcc agataactgc
     1621 ctggcaccag aatcatactg gagttaacct ggcgctgagg cttagtcatt gcggtgcctg
     1681 tatcaatgct gcgtacacca tcaacattga aaaaatactc gtacaggttg cctttcagga
     1741 taggtgtgcg ccacgaccag actcctgctt cgtctttggt catcgggtga atattgtccg
     1801 gaaccggaac acccaccact acagagacat tttttgcccc aggggcaaag tagcgaaagg
     1861 tcacgctgtt atcggcattg acctgagtca catactgttt caccgggata gtgggtgccg
     1921 ggctggctgg catatccgct gctatggccc actgagcaga aataccgctg gcaatagcca
     1981 gcgttaaagc agcaattttt atattcatcg taatattcct ttcattaagt tccattacca
     2041 ccagatttca gcctgggcac cgacagcaaa ctgggcgtct ttattgtctt cgaaggtgaa
     2101 gccatccagt tcgttttcca gggctttgat atacgtggcg tagaaacgaa tttccagacg
     2161 cgaggtcaac atactggtat tgactttaaa ggtatggaag agcgtggttt tataaccaga
     2221 ctcattaaat ttattactat tcgcatcttt gttttgctgg gtgaaatagc ccagttcaac
     2281 acctgtctgg ttatattggt cccaaatata ggccgggcga acaaccgcac gaatagattc
     2341 gaaatcagag tgtgcgcctg tttcgtagct ataaatattg ttaccgaagg agtaaacaat
     2401 cgcgttagcc acaataaaat gatcgccgat ataggcttcg ccctgcgagg tcagacgaac
     2461 tgctgttccg ccggtgtgat caccataata acgaccatta aaggtggtaa atggacttgc
     2521 gccagcataa cggccaaagt tcctggcgat agagttattc gcaaccagga aggagaattc
     2581 gttgaagcca cctttatcaa atttctgcgt taaagatgtg ccgaacatcc aggtatcttt
     2641 ccagtcataa tacccgttat tatcctgatt atctttttcc gatgcgcttt cgtttgccgt
     2701 gacataacga ccactcacca ttaaggtcgc tttatcccat aacgggatat ctttatagcg
     2761 taaatcaata gtatgggtat taatctgctg tttgttttgc aggctgcgat cgtaatcatc
     2821 gacatcttcg cgaaccagcg cgatatcaat tttacccgga ccgactttcc agttttccag
     2881 acctacaccc gctgcagcat cagtacgctg cgttttccag tccagcatct ggatttcaat
     2941 tttcggcgca ccgtgtttac ccacccagaa atcagcctct ggcgcaaagg gcaggaaacc
     3001 tttggtggta acgtacatat cggagaactg cataaagttc tcgccaccgg cattatcgcc
     3061 aaaccagccg gtagagtact gctgaccaac gttaccatct atcatcacaa cggcatcaac
     3121 ccgtttgccg ttttcgttgt agacacgttg ttttaactgc aaatcaaacc agccggagta
     3181 ttcgttacca aagcggccca gagaaccaat cgcccatgat ttaggtgaac catgagaggc
     3241 ggtcccccaa ccagaacggt aatagccgtt gtaactaaag ccaatttcat ctttcacaaa
     3301 cttactgaaa tctttcagag tcatggaaga ataaatcgag cctgtctcac tggcattttt
     3361 ctcttcattt ttgaccagta cagcatcagg ttttgcggca ctactggtcg ggaacggatt
     3421 ggcggcatac ccttgatcat tcgtacttac gctacgattc accgtcgctg gcgtatattt
     3481 tttcttctct tcatctttat actttttgag ttcgctttgc gtttctctta acgctttttc
     3541 taataactca aggcgttgtt ccaccgttaa tgattcagcc aatgattgtg cagaaaaggc
     3601 taacggtgcc attaataaga tggcagaggt aataagattt cgtctaaaca taatatccct
     3661 ttatggtgca aagaaagaat taacgtatcg catccagact gttctgaatg cgacgataat
     3721 taaggtgctt tattggttat ttttttaatg acagcccccg cgttttgatc acttctgcat
     3781 accatccgaa gcttttctta cgcgttcttg tgaggcttcc ttcgccatta tcatcacgat
     3841 ccacataaat aaagccgtag cgcttagaca tttgtgaatg agatgcactg actaaatcaa
     3901 ttggccccca actggtgtac cccataatat ccacaccatc ggcaatcgct tcatttacct
     3961 gtaccaggtg atcgtttaaa taggcaattc gataatcgtc ctgtatcgaa ccatccgctt
     4021 caacgctgtc ttttacgcct aatccattct cgacaataaa taacggtttt tgataacgat
     4081 cccaaagcgt atttaacaga acccgtaatc caaccggatc aatttgccac ccccactctg
     4141 aacttttcag atgcggattg gggatcatat tcagtatgtt gccctgcgca tttttattaa
     4201 tgctttcgtc gtgggaaaca caaccagtca tgtaataact aaaagagatg aaatctacag
     4261 tatgttttaa atcttctgcg tcactttcag tcatctcaat ggtgatattg tggtcgcgga
     4321 agaaacgctg catatagccg ggatattggc cacgcgcctg aacatcacca aagaacatcc
     4381 agcgccggtt ctcttccatg gcctgcaaca tatcctgtgg ctggcaggtg agggggtaaa
     4441 ccagcccacc gagaagcata ttgccgattt tcgcttcggg gatcaggcta tgataggctt
     4501 taactgcccg cgcactggca accagttgat gatggatcgc ctgataaact tccgcctcgc
     4561 cactctcttc tgccagcccc acgcccgtga atggcgcgtg taacgacata ttgatttcat
     4621 taaacgtcag ccataacgct actttatgtt ggtagcgggt aaagaccgtg cgggcgtaat
     4681 gctcgaagtg atcgatgacc gcccgattag cccaaccgcc gtagtttttc accagcccat
     4741 atagcatttc gtaatgggat aacgttacca gcggcttgat ccccgcctgc gccatttcat
     4801 caaacagccg atcgtaaaac gctaaccccg cttcattcgg ttcggcttcg tcgccctgag
     4861 ggaaaattcg cgcccaggca atggaaatac gcagacaggt gaagcccatc tcggcaaata
     4921 acgcgatatc ttccgggtaa cggtaataaa aatcgatggc gacatctttg atattctctt
     4981 tccccaggat gcgcggttcc atttttccca ttacgccatg aggctgtaaa tctgaggtcg
     5041 tgatcccttt gccatcttcc agccaggcac cttccacctg attggcagct gttgcaccac
     5101 cccaaagaaa tgtttctgga aatgctttca taattaactc cttttatcgt tagcgaatga
     5161 tggataacag cggttcacct gcgcttatct gcgccgtgcc gtggggtaat acgtctgtaa
     5221 aatcatcgct attactgatt aataccggcg tcgtcagatc aaatccggcc tcgcgaatag
     5281 cagggatatc aaaagaaatc agccgatcgc ctgtattgac cttgtcaccc acgttgacgt
     5341 gagcggaaaa gaatttgccg tccagtttta cggtgtcgat accgacatga atcaggatct
     5401 ccacaccatc atctgactca atgccaatgg cgtgtaatgt ggcgaacaac gaagcaattc
     5461 gacccgcaac cggagaacgc acttcaccaa ccgagggcag aatggcgata cctttaccca
     5521 acaggccact ggcaaacgtg gtatcagcga cgtgaatgag cggcacaatc tctcccgtca
     5581 tcggtgaaca gataccgccc tgctcaggtg gtgtaataac ctctggtgtt ttctcttgcg
     5641 gggcaccctg cgctggctga cgtttactgg tgatgaaatg aagcatcaac gtaccaacaa
     5701 atgcgcaacc tatggcaatg acaccgccaa taacgctggc ccagacggtg aaatcaatcc
     5761 ccgttgacgg gatggtctgc atgaaggtga aaatacttgg caaaccaaag gagtaaactt
     5821 tcgtttgcgc gtagccaatg atggtggccc ccaatgctcc actgatacag gcgataacaa
     5881 aggggtactt acgcggcagg ttgacgccat ataccgctgg ttcggtgata ccaaacagac
     5941 ccgtcaacgc cgctgatccc gccaccactt ttttctgcgc atcgcgttcg cagaggaaga
     6001 cgccgagcgc cgccccgacc tgcgccataa tggcgggcat taacagcggg atcatggtgt
     6061 cgtagcccag cacggtgaag ttattgatac acagcggcac caggcccctg tgcagtccga
     6121 acatgacgaa gatttgccag aagccgccca ttaccgcgcc cgcaaatgca ggaaccgcct
     6181 gataaagcca gagataaccg gcggcaatca tttcgcttat ccaggttgat agcggcccca
     6241 ccagcagaaa ggtgacgggt gtgataacca tcagacatag caatggtgtg aagaaatttt
     6301 tgattgccga cggtaaccac gcattaagtc ggcgttccag aatgctgcac aaccaggcag
     6361 aaaaaataat gggaataacc gatgacgagt aattcaacaa tgtgaccgga atacccagga
     6421 aatccagccc cggcgcatcc gctttttgcc cgttctcgaa ggcagtcaga attaatggat
     6481 gcactaacgc tccaccaatc accatggcag taaatggatt accgccgaag cgtttgcccg
     6541 cggtgtatcc caggattatc gggaagaacc caaacaaggc atcactggcg ctgaataaaa
     6601 ttaaataagt accactttgt tcggtcgtcc actgaaaagt gagcgccaga gccagcatac
     6661 ctttcaggat cccggttgcc gccatcagac cgatcagagg cgtaaaaata cctgaaataa
     6721 cataaacaaa gcggtttagc agattacctt tctcatcatt ttccggtgcc tgttgcgctt
     6781 tttcgccaag gcctgccacg ctgttaaccg ccaggaagac atcggccaca tggttaccta
     6841 tgaccacctg aaactgacca ccgctttcca ccaccataat aataccgggg gtctttttca
     6901 gtacctcagc ttgcgctttg ctttcatcct ttaacttaaa acgtaatcgc gttgcgcaat
     6961 gcatcagact cacaatgtta tctgcgcccc cgactcctgc gactattttt ctggctaact
     7021 ccgtcataac ttgccctcta ccgctttgcg gcaaaactcc aaaaaaaaac ctgaaaaaaa
     7081 cggcctgacg tgaatcaagc aatttttttc aggttttgcc cgcttagtgc ggtaacaatc
     7141 ctttactcag taataatatt tcagtgttct ttgcgcacgc gctctatatt tatggctaaa
     7201 aacataatct ctgcgggtga aattttacgt tgatactgca aaccaataaa aatggcgatc
     7261 cgttccgcac attgccatgc ttgcgggtaa ttttgtttta ctgcttgttg taatgattca
     7321 tcactatcgt taattgaagc atgttcaaga atacgccaag ataaaaactt cagatgtgta
     7381 accagtcgct gataactcaa gctttcttcc tggtaattaa ggctgaactg aaattttatt
     7441 aattgcagca tttcgcgcat taactgcgtg acacctgcaa catcctccat atttccgctc
     7501 atttgggcac tgaccagatg catggcaata aagcccactt catctttcgg taactgcacg
     7561 cccaaccgtt tatcaataat ggttaacgct tcttccccta gctggaactc tttcgggtaa
     7621 agccgctgga tatcccacag caacacgttt tgctgaaagc gtttaatcgc aaactggcaa
     7681 tggtcagtta gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa
     7741 gagataagac gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt
     7801 aatcgcccgt tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta
     7861 attctttcgc cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc
     7921 tgttgatcat caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc
     7981 atagcaagga ccttttttat aaacaaaaaa aaaccgactt caccagtatt ctctggttat
     8041 gtcaggtttt gcctgcgaat gcagtaacaa tccagtcatt tattaatggt ttttataacg
     8101 aacatccagg ttcggaaatt aatttaattg cgtgcagaga aaatagcaat gcgctattga
     8161 taaaaatatg accatgctcg cagttattaa ctttatgtaa ttttaggaat ttataaagtt
     8221 atatataaca aatcccaata attaagttat tgggatttgt ctggtgaatt atttgtcgct
     8281 atcttttccc gccagcagtt tatccagctc atcaccgcct acatgacgga aatcctgacc
     8341 cttcacgtag tagaagataa actcacaaat attctggcag cggtcgccga tacgttcgat
     8401 tgaacgcgca cagaacagcg cggtaagtac gctcggaata gtgcgcgaat cttccatcat
     8461 gtaggtcatc agttgacgaa caataccttc gtattcctga tcgacttttt tatcttcacg
     8521 ataaatacgt accgcttcgt caatgtccat ccgcgcgaac gcgtccagca cgtcgtgcag
     8581 catctggatg gtatgacggc ccagcgactc cagacttacc agcaacggct gatgctgctg
     8641 ggagaatttc tccagcgcag tacggcagat tttgtccgcc acgtcgccaa tacgctccag
     8701 ctcggcaatg gttttactga tcaccataac cagtcgcagg tcgctcgccg tcggctgacg
     8761 tttggcgata atgcgcacgc aggcttcatc gatcgccact tccatcatgt tgacgttctt
     8821 gtcgccttcg atcacgcgct tcgccagatc gctgtcctgg ttatgcatcg cggtgattgc
     8881 atcagaaagc tgctgctcca ccatgccgcc catggtcatc acctgcgtgc ggatactttc
     8941 cagttcggcg ttgaactggc cggaaatatg tttattaaga ttgagactgt ccataacgca
     9001 ctcctgaatc aaccgtaacg accggtgatg tagtcttctg tttgtttctt cgctggcttg
     9061 gtgaacagat cgtccgtgtt gctgaactca atcaattcgc ccaggtacat aaatgccgtg
     9121 tggtcggaac aacgcgcagc ctgctgcatg ttgtgggtga cgatcaccac ggtgtaatcc
     9181 tgcttcagtt cggtgatcag ctcttcaata cgcccggtag agatggggtc gagcgccgaa
     9241 cacggttcgt cgagcagcag cacttccggg cgaatggcga taccacgcgc aatacacaga
     9301 cgctgttgct gaccaccaga gagagagtaa ccgctctggt gcaatttatc tttggtttcg
     9361 ttccacaatg cggctttggt caatgcccac tgcacgcgct cgtccatgtc ggcacaggag
     9421 agcttctcaa acagacgaac gccaaaggcg atgttgtcgt agatggacat cgggaacggc
     9481 gtcggtttct ggaacaccat gcccactttc gcacgcagca gggcgatatc ctgagagttg
     9541 gtcaggatgt tgtcgccatc aagcagaatt tcaccttccg cacgctgctc cgggtacagt
     9601 tcaaacattt tgttgaaggt acgcagcagc gtcgatttac cgcagccgga cggcccgata
     9661 aacgccgtaa cctggttttt ggcgatatcc aggttgatgt ttttcagggc atggaatttg
     9721 ccgtagtaga agttcaaatt acgaacctga attttactcg gggcagtttc aaccatactc
     9781 attgcaatct tttcctcatc gtacctgatg cttttcgcta atcaggctta ccgtagggcc
     9841 gggtaaggcg ttcacgccgc atccggcaaa gtgttaaccg tgtttattct tcgcaaaaac
     9901 aacgcgcgcc agaatgttca gcagcaggac gcacagggta atgatcaata ccccggccca
     9961 ggccagttgt tgccattccg caaacgggct catcgcaaac ttaaagatcg tcaccggcag
    10021 gttggcgatc ggctgcatca tgtccgtgct ccagaactgg ttggagagcg cggtaaacag
    10081 cagcggcgcg gtttcaccgg caatacgggc aatcgccagc aggataccgg tcataatccc
    10141 ggacaccgac gctttcagcg taatcgcgga gatcatcttc cacttcggtg tacccagcgc
    10201 ataagccgct tcacgcaggc tgtccggcac cagtttcagc atgttctcag tggtgcggat
    10261 aacgatcggc acctgcaaca gcgccagcgc aattacgccc gcccagccgg agaagtgctc
    10321 catctgtgcc accacgatgg tgtagacaaa cagaccaacc acaatcgacg gcgcggagag
    10381 cagaatgtcg ttaatgaagc gaatcacttc tgccagccag gatttacgac catattccgc
    10441 cagataaatc cccgccataa tgcccagcgg cgtaccgaat accgtggccc acaaaattaa
    10501 cagcccgcta cccgccaggg cgttcgccag accaccacct tccgtattgg gcggcggcgt
    10561 catttcagtg aacagcgcca gcgacatacc gtcgataccg cgagtgatgg tggacattaa
    10621 aatccagatc agccagaaca ggccgaaggc catcgtcgcc atcgagagcg tcagcgcaat
    10681 acggtttttg aggcggcgac gcgcctgcat tttgcggcga gattcagcca gcgccgcagt
    10741 ggtttgcatt tcaaccatag ccattagcgt gccccctcat tcttagccag gcgcataatc
    10801 ataaacttcg atgcggcgag gacgatgaag gtaatcacaa acaggatcag gcccagttcc
    10861 atcagtgcgg caacgtgcag accggattcc gcttccgcaa attcgttcgc cagcgcagag
    10921 gtgatactat tgcccggcat gtacagcgag gcgctgtcga gctggtaggt gttaccgatg
    10981 ataaaggtca ccgccatggt ttcaccgagc gcgcggccca gccccagcat gatgccgccg
    11041 ataacaccat ttttggtgaa cggaagaacg atacgccaga taacttccca ggtggtgcag
    11101 ccaataccgt aggccgactc tttcatcatc accggggttt gttcgaacac atcacgcatt
    11161 accgccgcaa tgtacggaat aatcatgatg gcgaggatca cgcctgccgc gaggataccg
    11221 ataccaaatg cggggccaga gaacagcgcg ccaacaatcg ggatattcga catgatattg
    11281 ccgaccggct cctgaaagta aacggcgaac agcggcgcaa agataaacag gccccacatg
    11341 ccgtaaacga tacttggaat ggctgccagc agctcaatgg cgatacccag cgggcgtttc
    11401 agccagccag gcgcaagctc agtcaggaac agggcgatac cgaaactcac cgggacggcg
    11461 atcagcagcg cgataaacga agtcaccaac gtaccgtaga tcggcaccag cgccccgtag
    11521 atatcgttcg gtgcatccca ctctttggtc cataggaaag ccagaccaaa tttctgaatg
    11581 ctcggccagg aggagatgat cagagagaca ataatgccac ccaacatcaa tagcacaatc
    11641 agcgccgcca gttttaccag cacgctgaaa attatgtcgc cctttttacc cggtgggtta
    11701 aaagcgggct tggttgcagc cataagttac tcttcagtta aacgcgttta caaagtcatt
    11761 ttgtaatgcc ggatgcggcg taaaacaccg tacccggcct ggagttttat tagtacagcg
    11821 gcttaccgct actgtcttta atattggtct tccacgcagc gcgaacctgt tcaactacac
    11881 tatccggcag gctggcgtaa tccaggtcgt tcgcctgttt agccccggtt ttgtacgccc
    11941 agtcgaagaa tttcagcacc tctgtgcctt gttctggttt cttctgatct ttgtggatca
    12001 gaatgaacgt ggtagaggta ataggccatg catcttcgcc tttctggttg gtcagatcct
    12061 gagcgaaggt tttgctccag tctgcacctt ttgctgcatt agcgaagttt tcttcggtcg
    12121 gactaaccgg tttaccatca gcggagatca gtttggtgta cgccaggttg ttctgcttcg
    12181 cgtaagcata ttcaacataa ccaattgcac ccggcagacg ctgaacgaac gcggcgatac
    12241 cgtcgttacc tttaccgccc agaccgatcg gccattttac ggtagagcca gtaccaacgt
    12301 tgtttttcca ctcttcgttc actttcgcca ggtagctggt gaagacgaag gaagtcccgg
    12361 agccatctgc gcggcgtact acagcaatgt tttgtgaagg cagtttcaga cccggattca
    12421 gtttggcgat ggcttcatca tcccacttct tgattttgcc caggtagatg tcgccgaggg
    12481 ttttaccatc cagcaccagt tcgccagact tcagccctgg aatgttaacc gccagcacca
    12541 cgccgccaat cacggtcggg aactggaaca gaccttcctg agccagtttt tcgtcagaca
    12601 gcggcgcgtc agaggcacca aaatcaacgg tattagcgat aatctgtttt acgccaccgg
    12661 aagaaccgat accctggtag ttaactttat taccggtttc tttctggtaa gtgtcagccc
    12721 atttggcata caccggcgca gggaaggttg cacctgcacc tgtcaggctt gcttctgcaa
    12781 acacagagaa agcactcatc gataaggtcg cggcgacaac agttgcgacg gtggtacgca
    12841 taactttcat aatgtctcct gggaggattc ataaagcatt gtttgttggc tacgagaagc
    12901 aaaataggac aaacaggtga cagttatata taaggaatat gacattttta tgacagaaag
    12961 gaattaccaa ataacttact gattttaaat gtgaagatga aaatcccaca tgccatcttt
    13021 ttgtcatgat gcatttaaaa gtgataaaga aatgggtgag atgacaacga tgtgatgaac
    13081 aatcgttgtc agacctatta gcgcacaatc tcaacagtta tcgtcgctgt tgcagtaaat
    13141 tgccccgggg ctgggcgagc tccagtaagg ctcgcaggag cagctgaaaa agttacacta
    13201 cctgattttt caccatacac tgttgattta tccatgtctg ttggcaactc accaccgttc
    13261 actttgattt ccttattatt tttgtcgtaa atttttatcc ccacgttaga gtttgaagtt
    13321 acgaccattg aattgtccgt attgggttcg gtagcataaa aagacgtact gatatcatcg
    13381 gtattcagta tccccaggtt ttcacactca atagtgactg ttttagtaat tttatgtgtt
    13441 gttaccgcac ttccaacaac agatgaaaaa tctgcagctg gtatttcttt aaaatcaaaa
    13501 caaactggct ttaaattatt tatttcgcaa ctttgcggag ctgtaatatc gccccgaata
    13561 taaacatcag ccatcggctg tgagtaatcc accggggtgt cacggctaat tgttccatac
    13621 aatgtcacta tctttgtttc aggaataatc aattttcctg caaatgtctt cttgatataa
    13681 aaatatacta tcgctttagc cccactggat atacttaaag gttcaatgcg gttgcaattg
    13741 tactttgtat taccagacgg gttgttagca tgatattcaa aaggtgcatt cacataccca
    13801 accccaagaa tatctatagc aatcccaaca tcaaggttcg gattaagatc ataataatta
    13861 atattagagt gaaccccagc ataagttaat gcctggttta tttttgacgt ataatagata
    13921 taggggaatt ctcctacgtt accagtatca caatgacagc gtccaagata tgaggatcca
    13981 ttagttacag tggtgttaaa tgtcgttcca accttatttt cgttcgcggt aataagatat
    14041 cccg
//
LOCUS       DAJVRL010000098        13963 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVRL010000098 DAJVRL010000000
VERSION     DAJVRL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.098"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OQN02_003107"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OQN02_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1643813.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OQN02_003108"
     CDS             2219..2767
                     /locus_tag="OQN02_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1643814.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OQN02_003109"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OQN02_003109"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1643815.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OQN02_003110"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OQN02_003110"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1643816.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OQN02_003111"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OQN02_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1643817.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQN02_003112"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQN02_003112"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1643818.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OQN02_003113"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OQN02_003113"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1643819.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OQN02_003114"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OQN02_003114"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1643820.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OQN02_003115"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OQN02_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1643821.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="OQN02_003116"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="OQN02_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCS1643822.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3552 a         3562 c         3525 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DAJVRL010000099        13959 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVRL010000099 DAJVRL010000000
VERSION     DAJVRL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.099"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="OQN02_003117"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="OQN02_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1643823.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="OQN02_003118"
     CDS             complement(1604..1777)
                     /locus_tag="OQN02_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1643824.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="OQN02_003119"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="OQN02_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1643825.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="OQN02_003120"
     CDS             3415..3744
                     /locus_tag="OQN02_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1643826.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OQN02_003121"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OQN02_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1643827.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OQN02_003122"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OQN02_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1643828.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OQN02_003123"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OQN02_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1643829.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OQN02_003124"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OQN02_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1643830.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OQN02_003125"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OQN02_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1643831.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OQN02_003126"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OQN02_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1643832.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="OQN02_003127"
                     /gene_synonym="adhC"
                     /pseudo
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="OQN02_003127"
                     /gene_synonym="adhC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
BASE COUNT         3662 a         3454 c         3246 g         3597 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccaag tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggtta accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgagcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga caacgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttaccccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtataacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttttcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaagct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaaacagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcaga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttaag ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttctgg
//
LOCUS       DAJVRL010000100        13798 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVRL010000100 DAJVRL010000000
VERSION     DAJVRL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.100"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OQN02_003128"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="OQN02_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1643833.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OQN02_003129"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="OQN02_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1643834.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OQN02_003130"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="OQN02_003130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS1643835.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OQN02_003131"
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="OQN02_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS1643836.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OQN02_003132"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="OQN02_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS1643837.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OQN02_003133"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="OQN02_003133"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1643838.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OQN02_003134"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="OQN02_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1643839.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="OQN02_003135"
     CDS             7490..7639
                     /locus_tag="OQN02_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643840.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="OQN02_003136"
     CDS             7840..9423
                     /locus_tag="OQN02_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1643841.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OQN02_003137"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="OQN02_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1643842.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OQN02_003138"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="OQN02_003138"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1643843.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OQN02_003139"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="OQN02_003139"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1643844.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..>13798
                     /locus_tag="OQN02_003140"
     CDS             13382..>13798
                     /locus_tag="OQN02_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCS1643845.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3194 a         3640 c         3493 g         3471 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagtat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctggca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgagtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcaccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 catcctgctt ttttccacca gctcgctgaa catgacctgg acgaccatca atattgttga
    13681 agccgtggtc ggggcagtgc tgctacgtaa attgctgccg tggtataacc ccttgcaaaa
    13741 tctggctgac tggctgcgtc tggcattcgg cagcgccatt gttccgcctc tgttgggg
//
LOCUS       DAJVRL010000101        12906 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVRL010000101 DAJVRL010000000
VERSION     DAJVRL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.101"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OQN02_003141"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OQN02_003141"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1643846.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3223)
                     /locus_tag="OQN02_003142"
                     /pseudo
     CDS             complement(1806..3223)
                     /locus_tag="OQN02_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(3377..3721)
                     /locus_tag="OQN02_003143"
     CDS             complement(3377..3721)
                     /locus_tag="OQN02_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCS1643847.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3721..4647)
                     /gene="glsB"
                     /locus_tag="OQN02_003144"
     CDS             complement(3721..4647)
                     /gene="glsB"
                     /locus_tag="OQN02_003144"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1643848.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4711..5646)
                     /locus_tag="OQN02_003145"
     CDS             complement(4711..5646)
                     /locus_tag="OQN02_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCS1643849.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5726..6841
                     /gene="yneK"
                     /locus_tag="OQN02_003146"
     CDS             5726..6841
                     /gene="yneK"
                     /locus_tag="OQN02_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS1643850.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6991..8181
                     /gene="ydeA"
                     /locus_tag="OQN02_003147"
     CDS             6991..8181
                     /gene="ydeA"
                     /locus_tag="OQN02_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1643851.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8206..8871)
                     /gene="marC"
                     /locus_tag="OQN02_003148"
     CDS             complement(8206..8871)
                     /gene="marC"
                     /locus_tag="OQN02_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1643852.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9083..9517
                     /gene="marR"
                     /locus_tag="OQN02_003149"
     CDS             9083..9517
                     /gene="marR"
                     /locus_tag="OQN02_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1643853.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9537..9920
                     /gene="marA"
                     /locus_tag="OQN02_003150"
     CDS             9537..9920
                     /gene="marA"
                     /locus_tag="OQN02_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1643854.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9952..10170
                     /gene="marB"
                     /locus_tag="OQN02_003151"
     CDS             9952..10170
                     /gene="marB"
                     /locus_tag="OQN02_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1643855.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10201..11100)
                     /gene="eamA"
                     /locus_tag="OQN02_003152"
     CDS             complement(10201..11100)
                     /gene="eamA"
                     /locus_tag="OQN02_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCS1643856.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11364..12482
                     /gene="ydeE"
                     /locus_tag="OQN02_003153"
     CDS             11364..12482
                     /gene="ydeE"
                     /locus_tag="OQN02_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCS1643857.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12609..12704
                     /gene="mgtS"
                     /locus_tag="OQN02_003154"
     CDS             12609..12704
                     /gene="mgtS"
                     /locus_tag="OQN02_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCS1643858.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3399 a         3296 c         2951 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaatca tcggtcagcg
     1921 ttcggtacga tgcgcgacca ctaccgacgc caatacttac cgtgagataa agcgtttttt
     1981 gttgccaggt gaatggttgc agttcaacgc ctttacggat tttttccgcc attagcagac
     2041 catcgacagg attcaccgac ggcactgcaa cagcaaattc ttcgccgccc atgcgcgcca
     2101 ccagcccctt atcaccgaca atcttctgaa tatgccgggc aaacacgctt aacactttat
     2161 cgccacattc atgtccatag ttatcgttaa tgcttttgaa atagtcgata tcaagcagca
     2221 tgaccgtcag atgttgtgtc tgtttcagcg atggactttt cagcgcctca taaagaccgg
     2281 accgtgagta aacctgagtc agaaaatcaa agtcggctcg cagcgcaact tgcttcatta
     2341 gcaaattgat cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc atcgtggcaa
     2401 taccgagacg tgcggagaac atttccggaa tggagaacgg cgaaccgacc gagatatcaa
     2461 tcaccgaatt tgccaccagt acgatttcca ccgcaccggt gacaaaggtc aacagacatg
     2521 ttacctgcgg cgtatagcgc actgcacacc agattaatgc aggcagcggg aacgccagac
     2581 tccccgctcc gccaatgact accgaggcaa taaccgacac aataagcgcg atggcgggca
     2641 tcatctgttc tgctttaaag cgcggcagca ctccaggaat agccaacgtc agcatacaag
     2701 gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga
     2761 aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg
     2821 caatcagaca gtaattaaat agccgtaaag cacttactgg ctcatactta tttttcccca
     2881 gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat
     2941 tgataaccag tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca
     3001 cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca aacacccccg
     3061 ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac
     3121 caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac
     3181 cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac
     3241 aattgcgggt ggcaattatc tcaacgtgat aagtaaattc tcataaagga tagcatgttc
     3301 gctaacagcg gcggcaaata aaggtaccct gggaatgcta ttttatcgtt ttccgcaatt
     3361 ctcatggctg attcatctat acaaccagcc agtgatagat taccgccacg atataacgtt
     3421 gttgttcttc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc agacaaccgc
     3481 gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt
     3541 gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat
     3601 gttgttcgat gactggcgcg cctttctcca ggcaatactg acgctctttc atgcccagac
     3661 gaaagcgaga gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agatactgca
     3721 ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag
     3781 cgagttacct gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac
     3841 aatcgccaca ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa
     3901 ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt gccgcgcctg
     3961 cattggcgtc accacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac
     4021 aaacgtccgg gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg
     4081 cagaacggtt gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc
     4141 cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt atgaaatatc
     4201 agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttagtcg
     4261 cccttgcaac atatcgcaga ccaccagcgc accggtatta atgaacggat tacgcggaat
     4321 accctgttcc atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc
     4381 gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac
     4441 tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc
     4501 gtcaacggta cagatagcaa tccccaatcg ggaaccgtct actgtagcca gcgccggaat
     4561 ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa
     4621 aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc
     4681 tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca ctgtctggat
     4741 attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac
     4801 gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca
     4861 ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga
     4921 aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaatggt
     4981 gattgcagca acggggccaa acatttcttc ccgaaacgcg gtcatttctg gggtaacatt
     5041 cgccagaacc gttggcggat agtagttacc tgctccagcc atcttttccc cgcccagtaa
     5101 caaacgcgca ccctgcgcca gggttttctc cacctgatga tgcagctcat cacgtaaatc
     5161 aaaacgagcc attggtccga gagcgttctc ttcatcacgg ggatcgccca ttttcaaggc
     5221 tgccgcagct gacacaaaac gttcggtaaa tgccgaagca attccctctt cgataataaa
     5281 gcgttttgcc gctgcacaaa cctgtccggt attctgataa cgtccggcta ccgccgcttt
     5341 aaccgccagt tccagatcgg catcgttaag cacaataaat ggatccgaac cgcccagttc
     5401 cagtacgcat tttttcagtg ccgctccagc acgaacactt ccggtcaccg tgacagcagc
     5461 aatgcgcgag tctttaatca tctgactgac accgtcgttg tcggcattca gccagccata
     5521 tacgccttgc gggattcccg catctttaaa cacttgggca atgagctgag cacagcccat
     5581 cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg gaacagcgcc
     5641 acgcatcacc tgcctttgat agttcttgca gctcaaatgc cctatatagt gatgttccga
     5701 tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc
     5761 tcttcctgat gattttcccg ctcgtaatat tgcacctcaa gtaaaagaag ttttaaagga
     5821 ttttattgat gcactcagta ccataatatg tgatgaagag tggcgcacgt ctttaaacac
     5881 caactcagcc acaaaaaaga tatttaacaa ccttgataac ttatcatata ttcagaggac
     5941 ttcttttcgg ggtaacgaca cactatacaa tgaaaaggtt cagtttaaac tcacttatcc
     6001 tgtcaagaat ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc
     6061 tatttattta gataattttc gccatgatgg agaaattaat attttttgcg ctcccaaccc
     6121 aaagcctgtc actatggggc gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt
     6181 tatgaatgat tttattgaac aatttccaat gatcaacctt ggcgccccca tcaaaagagc
     6241 gcatacacca catattgaac ccctgcctcc ggatcaccat accgctgcag attacttacg
     6301 ccagtttgat ttgcttgtcc tgaattttat ctctcgcggt aattttgtca tactcccccg
     6361 attatggaat aactctgagg ttcacagatg gttcgtcaat aaagatccta acttgatcac
     6421 cgccattctc gacataacgg acagcgaatt aaaagaggat ttattgcaaa gcctgatgga
     6481 ttcattaggt tctaacaaac atgtacaacc cgaagtctgc atctgcttct tatccctttt
     6541 agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca atatgttatt
     6601 gcactatcac caatttatga atcccaatga aagtgatctg aatgacgtgt tgatgccagc
     6661 atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aggaccctca aactgtttgt
     6721 aaaaaatgaa acgccgccaa aagttactca cgaagacctg gtgaaaaaca ggcctcgctc
     6781 ccctgtcaga ccacctatac ccgcaaccgc caaaacgcca gacctccctg aacgtcatta
     6841 aaccgtgatg ttaccgactc tctgactcgg aacagaatca gcgtcagaga aacggaaaac
     6901 gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc
     6961 actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg
     7021 gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt
     7081 gtccctgttg gcctgctctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc
     7141 ggcatcatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta
     7201 atgaccagtc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc
     7261 agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag tcgcattggt
     7321 gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat ccgtatggct
     7381 ccggccggga agcgggcaca ggccttgagt ttaattgcca ccggtacagc actggcgatg
     7441 gtcttaggtt taccgctcgg gcgcattgtg gggcagtatt tcggctggcg aatgaccttc
     7501 ttcgcgattg gtattggggc gcttgttacc cttttgtgcc tgattaagtt acttccctta
     7561 ctgcctagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg ccgcccggca
     7621 ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc
     7681 tatatcgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta
     7741 ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat
     7801 caatatgcgt ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg tctggcactg
     7861 ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggatc
     7921 gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgct
     7981 accgacgtcg cgatggcgct attctccggc atatttaata ttggaatcgg ggcgggtgcg
     8041 ttggtaggta atcaggtgag tttgcactgg tcaatgtcga tgattggtta tgtgggcgcg
     8101 gtgcctgctt ttgctgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca
     8161 ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat ggtacgtttt
     8221 aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca gcaggaaccc
     8281 catcaggcgg gagatcgctt caatgccacc cttgcccacc agccgcataa ttgcgccgga
     8341 gctgcgtagg cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac
     8401 catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat
     8461 catcgctatg gttcccggac cggcggtact tggcattgcc agtggtacaa aggcaatatt
     8521 ggcactgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg aatcaatcgc
     8581 tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat
     8641 tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccag cgtaatacgc
     8701 caccatcatg atggcaaata cgtacaccga ggccatcaac gactgacgat tacgttcggc
     8761 actgttcatg ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg ggttagctaa
     8821 cggcagcaac accaccagcc ccaggccaat tgctttaaac aaatctaaca ttggtggttg
     8881 ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc
     8941 actctttagc taaccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg
     9001 catcggtcaa ttcattcatt tgacttatac ttgcctgggc aatattaccc cctacaacta
     9061 attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc
     9121 attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta acgagtatct
     9181 gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc
     9241 gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag cactgacccg
     9301 tatgctggat cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc cgaatgataa
     9361 gcgcggcgta ctggtaaaac ttaccaccag cggcgcggaa atatgcgaac aatgccatca
     9421 attagttggc caggacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagttgc
     9481 aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaagaggt atgacgatgt
     9541 ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc gaggacaacc
     9601 tggaatcgcc actgtcactg gagaaagtgt cagagcgttc gggttactcc aaatggcacc
     9661 tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc cgcagccgta
     9721 agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc tatctggcag
     9781 aacgatatgg cttcgagtcg caacaaactc tgacccgaac cttcaaaaat tactttgatg
     9841 ttccgccgca taaataccgg atgaccaata tgcagggcga atcgcgcttt ttacatccat
     9901 taaatcatta caacagctag ttgataacgt gacaacgtca ctgaggcaat catgaaacca
     9961 ctttcatccg caatagcagc tgcgcttatt ctcttttccg cgcagggcgt tgcggaacaa
    10021 accacgcagc cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc atcgcaggaa
    10081 caaccaccgt ttgatttaaa tcacatgggt actggcagtg ataagtcgga tgcgctcggc
    10141 gtgccctatt ataatcaaca cgctatgtag tttgttctgg ccccgacatc tcggggctta
    10201 ttaacttccc acctttaccg ctttacgcca ccgcaagcca aatacattga tatacagccc
    10261 ggtcataatg agcaccgcac ctaaaaattg cagacccgtt aagcgttcat ccaacaatag
    10321 tgccgcactt gccagtccga ctacgggcac cagtaacgat aacggtgcaa cccgccaagt
    10381 ttcatagcgt cccagtaacg tcccccagat cccataacca acaattgtcg ccacaaacgc
    10441 cagatacatc agcgacaaga tggtggtcat atcaatagta accagactgt gaatcatgga
    10501 tgcggaacca tcgaaaatca gcgagacaac aaagaaggga atgattggga ttaaagcgct
    10561 ccagattacc agcgacatca ccgccggacg cgttgagtgc gacatgatct ttttattgaa
    10621 gatgttgcca cacgcccaac taaatgctgc agccagggtc aacataaagc cgagcatcgc
    10681 cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa tcgctaaggc
    10741 gatccccgcc aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc caagcacgat
    10801 agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca taccaaagtt
    10861 aatggcgcag aacagaaagg caaactgcgc aaaactgatg gttaatccat accccaagag
    10921 caaattcagt ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag cgaccagcat
    10981 aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac
    11041 aaaatttagc ccccatacga ccactaccag tagcgccaac accccatctt ttcgcgacat
    11101 tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt
    11161 taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata agtcgctata
    11221 cttcggattt ttgccatgcg tccgatggtt aataacaacg acgaggcaag aagggcgctg
    11281 gtagagcgtc gtagggataa gttcatttgt ctgtccggca gtgcgtttcg ttatgtttta
    11341 ttttagagat gtaaagaaat agcatggcta cgctgccatt tatgaccatt tacttgagtc
    11401 gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg ctcactattg
    11461 gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag aaacgctata
    11521 tgttactggc aattaccgcc ttcgccagcg gttttattgc cattccttta gtgaataacg
    11581 tgacgctggt tgtgctcttt tttgccctca ttaactgcgc ctattctgtt tttgctactg
    11641 tgctgaaagc ctggtttgcc gacaatcttt cgtccaccag caaaacgaaa atcttctcaa
    11701 tcaactacac catgctaaac attggctgga ccatcggtcc gccgctcggc acgctgttgg
    11761 tgatgcagag catcaatctg cccttttggc tggcagctat ctgttccgcg tttcccatgc
    11821 ttttcattca aatatgggta aagcgcagcg agaaaatcat cgccacggaa acaggcagtg
    11881 tctggtcgcc gaaagtttta ttacaagata aagcactgtt gtggtttacc tgctctggtt
    11941 ttctggcttc ttttgtaagc ggcgcatttg cttcatgcat ttcacaatat gtgatggtga
    12001 ttgctgatgg ggattttgcc gaaaaggtgg tcgcggttgt tcttccggtg aatgctgcca
    12061 tggtggttac gttgcaatat tccgtgggcc gccgacttaa cccagctaac atccgcgcgc
    12121 tgatgacagc aggcaccctc tgtttcgtca tcggactggt cggttttatt ttttccggca
    12181 acaacctgct attgtggggt atgtcagctg cggtatttac tgtcggtgaa atcatttatg
    12241 cgccgggcga gtatatgttg attgaccata ttgcgccgcc aggaatgaaa gccagctatt
    12301 tttccgccca gtctttaggc tggcttggtg ccgcgattaa cccattagtg agtggcgtag
    12361 tgctaaccag cctgccgcct tcctcgctgt ttgtcatctt agcgttggtg atcattgctg
    12421 cgtgggtgct gatgttaaaa gggattcgtg caagaccgtg ggggcagccc gcgctttgtt
    12481 gatttaagtc gaacacaata aaaatttaat tcagccttcg tttaggttat ctctgctaat
    12541 atctttctca ttgagatgaa aattaaggta agcgaggaaa cacaccacac cataaacgga
    12601 ggcaaataat gctgggtaat atgaatgttt ttatggccgt actgggaata attttatttt
    12661 ctggttttct ggccgcgtat ttcagccaca aatgggatga ctaatgaacg gagataatcc
    12721 ctcacctaac cggccccttg ttacagttgt gtacaagggg cctgattttt atgacggcga
    12781 aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt
    12841 acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc
    12901 tcaatg
//
LOCUS       DAJVRL010000102        12699 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVRL010000102 DAJVRL010000000
VERSION     DAJVRL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.102"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(104..742)
                     /gene="rutR"
                     /locus_tag="OQN02_003155"
     CDS             complement(104..742)
                     /gene="rutR"
                     /locus_tag="OQN02_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS1643859.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1030..2121
                     /gene="rutA"
                     /locus_tag="OQN02_003156"
     CDS             1030..2121
                     /gene="rutA"
                     /locus_tag="OQN02_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCS1643860.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2121..2813
                     /gene="rutB"
                     /locus_tag="OQN02_003157"
     CDS             2121..2813
                     /gene="rutB"
                     /locus_tag="OQN02_003157"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCS1643861.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2825..3211
                     /gene="rutC"
                     /locus_tag="OQN02_003158"
     CDS             2825..3211
                     /gene="rutC"
                     /locus_tag="OQN02_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS1643862.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3219..4019
                     /gene="rutD"
                     /locus_tag="OQN02_003159"
     CDS             3219..4019
                     /gene="rutD"
                     /locus_tag="OQN02_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCS1643863.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4029..4619
                     /locus_tag="OQN02_003160"
     CDS             4029..4619
                     /locus_tag="OQN02_003160"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCS1643864.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4630..5124
                     /gene="rutF"
                     /locus_tag="OQN02_003161"
     CDS             4630..5124
                     /gene="rutF"
                     /locus_tag="OQN02_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCS1643865.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5145..6473
                     /gene="rutG"
                     /locus_tag="OQN02_003162"
     CDS             5145..6473
                     /gene="rutG"
                     /locus_tag="OQN02_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCS1643866.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNTNLIMVAVTF
                     VLGAGDFALTLGGFTLGGICTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6555..6662)
                     /locus_tag="OQN02_003163"
     CDS             complement(6555..6662)
                     /locus_tag="OQN02_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643867.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7035..7631
                     /gene="wrbA"
                     /locus_tag="OQN02_003164"
     CDS             7035..7631
                     /gene="wrbA"
                     /locus_tag="OQN02_003164"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1643868.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7652..7879
                     /locus_tag="OQN02_003165"
     CDS             7652..7879
                     /locus_tag="OQN02_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCS1643869.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7917..9158)
                     /gene="agp"
                     /locus_tag="OQN02_003166"
     CDS             complement(7917..9158)
                     /gene="agp"
                     /locus_tag="OQN02_003166"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCS1643870.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9451..10707)
                     /locus_tag="OQN02_003167"
     CDS             complement(9451..10707)
                     /locus_tag="OQN02_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCS1643871.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10968..11888
                     /gene="cbpA"
                     /locus_tag="OQN02_003168"
     CDS             10968..11888
                     /gene="cbpA"
                     /locus_tag="OQN02_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1643872.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11888..12193
                     /gene="cbpM"
                     /locus_tag="OQN02_003169"
     CDS             11888..12193
                     /gene="cbpM"
                     /locus_tag="OQN02_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1643873.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12286..>12699)
                     /gene="torD"
                     /locus_tag="OQN02_003170"
     CDS             complement(12286..>12699)
                     /gene="torD"
                     /locus_tag="OQN02_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCS1643874.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3035 a         3245 c         3197 g         3222 t
ORIGIN      
        1 acgttcgctg agtgtgaata ccgctgccgc tggcggtaac gccagtttaa tgacgatcgg
       61 ttaaacaggt agccggagga tgttacaacc tcctccgtca tctttaacgt ggtcgaatcc
      121 cctcaataat aatccgctgc acgttttcaa ccgtttgatt gaaaaatacc tcatcgcgca
      181 acgtcgcgcc tgtcaccgcc tccacctgag gggcgaaatc ggcgtaatgt tgagtggaag
      241 cccaaatcat aaaaatcaaa tgctgcggat caatcggcgc gagtttgccg cttttgaccc
      301 aaccggcaat cagcgccgat ttctcatcaa ttaatgcctt caaatcgccc gtcagttcat
      361 ccattaacaa cggcgcgcct gccagcatct ccatacagaa caggcgcgaa gcctgcggat
      421 aatcgcgtga gacttccagc ttcagacgga tgtactcttt tatcgccgcc agcggggcga
      481 aatcttcacg aaacgctttt aacggtgcca gccagatatc gagaatctgc cgcagcacgg
      541 caatatacag cgcctctttt gacgggaagt aatacagcag attggttttt gaaacacccg
      601 ccaactctgc ggtctgctcc agtcttgtgc cgtgaaaacc gaattgtgaa aaagtgtcca
      661 gtgctgcgct aagaatcgct tttttcttcg cgcttactgc gcgcgaacgt ttacccgttg
      721 ttttcactgc gccttgcgtc atgcgctctc ccctcttgta gattggtaac aggatagcaa
      781 aacccccgcc cggcctcgac cttctgcact ctcatcgcgc acggatgcat gttttatgtg
      841 caactgtttt gaccgtttag tccacttttt accagatatt ttagataaca caatgctaac
      901 acattaataa cattcacttt ttaaaaactg gcatccgctt tgcaaacaag ccaatacgcg
      961 gctgaaaagg atatgcagga tgcagcgccc cgtctgactt tcactttacg agacgaagag
     1021 aggttgatga tgaaaattgg cgtattcgta cctattggca acaacggctg gctcatttcg
     1081 acccacgcgc cgcagtacat gccgaccttt gaactgaata aagccattgc gcaaaaagcg
     1141 gagcattacc atttcgattt cgccctgtcg atgatcaaac tgcgtggctt tggcggcaaa
     1201 actgagttct gggatcacaa ccttgagtcg ttcaccttga tggcgggtct ggcagccgtg
     1261 acctcgcgca ttcagattta cgccactgct gccaccttaa cgttacctcc ggcaatcgtc
     1321 gcccgtatgg ccgcaaccat cgactccatc tctggcgggc gttttggcgt caacctcgtg
     1381 actggctggc aaaagcccga gtatgagcag atgggtatct ggcctggcga tgactatttc
     1441 tcccgtcgtt acgactatct caccgaatat gttcaggtgc tgcgcgacct gtggggcacg
     1501 gggaaaagcg attttaaagg cgattttttc accatgaatg attgtcgcgt cagtccgcaa
     1561 ccgagtatcc ccatgaaagt gatctgcgcc gggcaaagcg acgctggcat ggcgttctcc
     1621 gcccagtatg ccgatttcaa cttctgtttc ggcaaaggcg taaatacacc cacggctttc
     1681 gccccgaccg ctgcgcggat gaaacaggcc acagagcaaa ccggacgcga cgttggctct
     1741 tatgtgttgt ttatggtgat tgccgatgaa accgacgatg ccactcgcgc caaatgggaa
     1801 cactacaaag cgggcgcgga tgaagaggcg ttaagctggc taaccgaaca aagtcagaaa
     1861 gatacccgct ccggtactga caccaacgtt cgtcagatgg ccgatcccac ttcggcggta
     1921 aacatcaata tggggacgtt agtcggttct tacgccagtg tcgcgcgcat gttagatgaa
     1981 gtcgcaagcg tgcctggtgc agaaggcgtg ctgttgacct tcgatgattt tctgtcggga
     2041 atcgaaacct tcggcgagcg cattcaacca ctgatgcagt gccgcgccca tctctctgcg
     2101 ctgactcagg aggtggcatg atgacaacct taaccgctcg accggaagcc attaccttcg
     2161 atccgcagca aagtgcgcag atcgtggtgg atatgcaaaa cgcttatgcc acgccaggcg
     2221 gctacttaga tctcgccggg tttgatgtct caaccactcg cccggtcatt gccaacattc
     2281 aaaccgctgt gaccgcagcg cgagcggcag ggatgttgat catctggttt caaaatggct
     2341 gggatgaaca gtatgtcgag gctggcggcc ccggctcacc gaattttcat aaatcgaacg
     2401 ccctgaaaac catgcgtaag cagccgcagc tgcaggggaa attgctggcg aaaggctcct
     2461 gggattatca actggtggat gaactggtgc cgcagcctgg cgatattgtg ctgccgaagc
     2521 cgcgctacag cggtttcttc aatacgccgc tggacagcat tttacgcagc cgtggaatac
     2581 gccatctggt tttcaccggt atcgctacca acgtctgcgt cgaatcgacg ctacgcgatg
     2641 gcttttttct ggagcatttc ggcgtggtgc tggaagacgc aacacaccag gcggggccgg
     2701 aatttgcaca gaaagccgcg ttgttcaata tcgaaacctt ttttggctgg gtcagcgacg
     2761 tcgaaacgtt ctgcgacgcg ctttctccca cgtcctttgc tcgtatcgct taaggagttt
     2821 agcgatgcca aaatccgtaa ttattcccgc tggcagcagc gcaccgctgg cccccttcgt
     2881 tcccggcacg ctggctgatg gcgtggtgta tgtctccggt acgctggctt ttgatcaaca
     2941 taataacgtg ctgtttgccg atgacccaaa ggcgcaaacc cgccacgttc tggaaactat
     3001 ccgcaaggtg atcgagacgg cgggtggcac gatggcggat gtgaccttca acagcatctt
     3061 tattaccgac tggaaaaatt acgccgcgat taacgaaatc tacgccgagt tttttccggg
     3121 tgataaaccg gcgcgattct acattcagtg cggactggta aaacctgacg cgctggtgga
     3181 aatcgccaca attgcgcata tcgccaagtg aggccgcgat gaaactttca ctctcacctc
     3241 ccccttatgc tgatgcaccc gtagtggtgt tgatttcggg tcttggcggt agcggcagtt
     3301 actggttacc gcaactggcg gtgctggagc aggagtatca ggtagtctgt tacgaccagc
     3361 gcggcaccgg caataatccc gacacactgg cagaagatta cagtatcgcc cagatggcag
     3421 cggaactgca tcaggcgctg gtagccgcag ggattgagcg ttacgcggtg gtcggccatg
     3481 cgctcggtgc gctggtgggg atgcagctgg cgctggatta tcccgcgtcg gtaactatgc
     3541 tggtcagcgt taacggctgg ctacgaataa acgcccatac gcgccgctgt tttcaggttc
     3601 gcgaacgatt actgtatagc ggcggcgcgc aagcatgggt ggaagcgcag ccgttgttcc
     3661 tctatcccgc cgactggatg gcggcccgcg cacctcgcct ggaggcagaa gacgcgctgg
     3721 cactggcgca ttttcagggc aaaaataatt tactacgtcg acttaacgcc ctcaaacgcg
     3781 ctgactttag tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc tgcgccagtg
     3841 atgatctgct ggtgccaaca gcatgttcca gtgaacttca tgccgccctg cccgatagcc
     3901 agaaaatggt gatgccctat ggcggacacg cctgcaacgt gaccgatccc gaaacgttta
     3961 atgctctgtt actcaacggg cttgccagcc tgttacatca ccgtgaagcc gccctgtaag
     4021 gaattgctat gaacgaagcc gttagcccag gtgcgcttag caccctgttc accgatgccc
     4081 gcactcacaa cggctggcgg gagacacccg tcagcgatga gacgttacag gagctttatg
     4141 ccctgatgaa atgggggccg acatcagcta actgttctcc ggcacggatc gtgtttatcc
     4201 gcactgcaga aggaaaagaa cgtctgcgcc cggcgctttc cagcggcaat ctgcaaaaaa
     4261 ccctgaccgc gcccgtcacc gctatcgtcg cctgggacag tgaattttat gaacggttac
     4321 cactactgtt tccccacggt gatgcccgca gttggtttac ctccagccca caacttgccg
     4381 aagaaacagc gtttcgcaac agttccatgc aggcggctta tctgatcgtc gcttgccgtg
     4441 cgctgggact ggataccggc ccgatgtcgg gttttgaccg tcaacacgtt gacgacgcct
     4501 tttttgcggg cagcacgctg aagagcaatc tgctgattaa tatcggctat ggcgatagca
     4561 gcaaactttt tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg ctgttgtaag
     4621 gagcaaacta tgaacatagt tgatcaacaa acctttcgcg atgcgatgtc ctgtatgggc
     4681 gcggcggtca atatcatcac cacggacggt ccagccgggc gcgccgggtt caccgccagc
     4741 gccgtctgca gcgtgaccga cacgccgccc acattactgg tgtgcctgaa tcgtggggcg
     4801 tccgtctggc cggtattcaa tgaaaatcga acgctgtgtg taaatacact gagcgccggg
     4861 caggagccgc tttcaaatct ttttggcggc aaaacgccca tggaacaccg ctttgccgcc
     4921 gcccgctggc agacgggtgt gaccggatgc ccgcaactgg aagaggctct ggtttcgttt
     4981 gactgccgta tcagccaggt ggtgagcgtc ggcacccacg acattctgtt ttgcgccatc
     5041 gaagcgattc atcgtcacac cacaccctac gggctggtgt ggttcgatcg cagttatcac
     5101 gcgctgatgc gccctgcttg ttaacctcct taaggagaca gctgatggca atgttcggtt
     5161 ttcctcactg gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg ccggatgaac
     5221 gattgccgtt tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg atgtttggcg
     5281 cgacggtgtt aatgccgatt ctgatggggc tggatcccaa tctttccatt ttaatgtcgg
     5341 ggatcggcac gttactgttt ttctttatta ccggcgggcg cgtccccagt tatctcggct
     5401 ccagcgcggc ttttgtcggc gtggtgattg ccgcgacggg ctttaacggt cagggcatta
     5461 acccgaatat cagcatcgcg ctgggcggga ttatcgcctg tgggctggtt tataccgtca
     5521 ttggcctggt ggtgatgaaa attggtacac gctggattga acgactaatg ccgccggtgg
     5581 tgacgggtgc ggtggtgatg gcgattggcc tgaacttagc gcctatcgca gtgaaaagtg
     5641 tatcggcttc ggcctttgat agctggatgg cggtaatgac ggtgctgtgt attgggctgg
     5701 tggcggtgtt tacccgtggc atgatccagc ggttattgat tctcgtagga ttaattgtcg
     5761 cctgtctgct gtatggcgtg atgaccaacg ttttagggct gggaaaagcc gtcgatttta
     5821 ctctcgtcag ctatgctgcc tggttcggtc tgccgcattt ttccactcct ggttttaatg
     5881 gccaagcgat gatgttgatt gcgcccgtgg cggtgattct ggtggcggaa aacttaggtc
     5941 atctcaaggc ggtcgccggg atgaccggac gcaatatgga cccgtatatg gggcgagcat
     6001 tcgtcggtga tggtctggca acgatgcttt ccggctctgt cggcggtagc ggcgtcacca
     6061 cctatgctga aaatatcggt gtgatggccg tgacaaaagt ctactcgacg ctggtgtttg
     6121 ttgcagctgc cgtcatcgct atgctgttgg gcttttcgcc gaaatttggc gcgctgatcc
     6181 ataccattcc ggccgcggtg attggtggtg catcaattgt tgtgttcggg ctgattgccg
     6241 tcgcaggcgc aaggatatgg gtacaaaacc gtgtcgattt aagccagaac actaatttga
     6301 ttatggtcgc agtaaccttc gtgctgggcg cgggtgattt tgctctcacg cttggcggtt
     6361 ttacgttggg agggatctgt acagcaacct ttggcgcgat tttactcaat gcgttattaa
     6421 gcagaaggtt ggttgacgtt ccaccgccag aagttgttca tcaggagcca taaacagaaa
     6481 accgccgggt taccggcggt gatgactttt tgacgagctt aagaaaaaaa tgcattcgtc
     6541 attgattagc ccggctagtt gtcgcttttg ccgtgactgc tcttgccacc ttttttacct
     6601 gcttctgatg ctctttcgcg gtcttctgca aaattgccgg aaccgcctcg atggtttgcc
     6661 attactgacc tccgtctggg tatagacatc atattgcata gaattaacgc ggcgtatggc
     6721 ggctgccata ttttcgctac gtgggactaa gcttagtgaa ctgacgcgga cagacccgcg
     6781 tttggtaata tcctgcaaca gcttcttagc gagaatagat atcatctcca ataattatcc
     6841 ataagccgct cttatgatta aaaaaacgcg atgagataaa aatgtgtcat tttgcgacaa
     6901 aattacgtgc ttgtgaaagt tgttataaat caaataagtg gttgtgaaat ttgcactctg
     6961 aaaaggacgt cttatcttta aataagtggt agcgaatcgc tacggaatag agataacacg
     7021 aggagtggtt agaaatggct aaagttctgg tgctttatta ttccatgtac ggacatattg
     7081 aaacgatggc acgcgcagtc gctgagggtg caagcaaagt ggatggcgca gaagttgtcg
     7141 ttaagcgtgt accggaaacc atgccgccgc aattatttga aaaagcaggc ggtaaaacgc
     7201 aaactgcacc ggttgcaacc ccgcaagaac tggccgatta cgacgccatt atttttggta
     7261 cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac cagacgggcg
     7321 gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt tccaccggta
     7381 ctggcggcgg tcaggaacaa accatcacat ccacctggac gacccttgcg catcacggca
     7441 tggtgattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca caggttcgcg
     7501 gcggtacgcc gtacggcgca accaccatcg caggcggtga cggctcacgc cagccaagcc
     7561 aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg gcagttaaac
     7621 ttaacggcta atcttcaaca ggaggatacg catgccaact caagaagcga aagctcatca
     7681 cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg ccattttcga
     7741 agtcgccggg tacgacgaaa aaatggcaga aaagatttgg gaagaaggta gtgatgaagt
     7801 gttagtcaaa gcctttgcta agaccgataa agattcgctt ttttggggcg aacagaccat
     7861 cgaacgtaaa aacgtttaac cagcgactcc cccgcttctc gcgggggagt tttctgttat
     7921 ttcaccgcct cattcaacac gctatcaaac ttatccatcg ggcagaaacc gttggcatct
     7981 atcgggcaac cgcttaattc cagcgtcacg cgctgcgcag gtgcctgcag ggttaacgca
     8041 tcggcattac gtaactgttc cgcactctgg tacacatatt caattttcat caaatcgcga
     8101 ttggctttgc tgtcatgcca acgctggaaa acgattttgc cgccaatcgg cgtgcgttcg
     8161 ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga ggctatgttg
     8221 gagtcgtgcc caactaacac cgtgattttc ggagcactgg cgcgatcggt gaccagggct
     8281 ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg tgaggtaaac
     8341 agactgtcct ggtaaccgtt tttcagcttc gacaatacct tccactgctg gtcagatttg
     8401 atttctcccc aggccacctg atccatcgga aaaccttcgt agtattgcaa agtaaacgca
     8461 tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg ttgatacttg
     8521 gcgctaaagg tatttttgcc atccaccagc gaacactgtt gtttctcttt acaggcaggg
     8581 gaatctttat agttaacgat tttttccagt agctggtagc tgtcggtaag ctggagtttg
     8641 ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga atcatcggtg
     8701 atcatcgggt taaaggttgg gtccatagtg cccatttttt cctggtgatg cacaggaata
     8761 tcacacccag ggaatgcacc agtaataaag aactgggcgg tagcgacggt acgttgcaga
     8821 ctattggcat aggcataaac ggtgtccggc ggcgggcatt cccccgattt cactatcccc
     8881 tgctctgcca gccattcacg catgtaatgg cccatataca cttcgagcac gccacctttg
     8941 gtggtgagtt gcccaccggg gacgtcccat tcgggccatt tattcggcgt cgactgctcc
     9001 agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat catgagcact
     9061 tgctgtagct gatagccttc cggtacggtt tgtgcctgag catttgaagc gagtaaaact
     9121 atccctgcca cagttgcggc gattagcgtt ttgttcattc ctggcacctc ttttgttatc
     9181 aatcattaaa gagtgtgaca gaaatatgac ttttttccgg gaacaggttc gcaaaatggc
     9241 ttgttgaacg cctgtttttc tatatacatc ttcaaaaaca ggcaaggttt gacgcaaact
     9301 atgcctgatt agcccctgcc tcgcgtacgc cgctgataat tgcacctgtg gctaaacaga
     9361 atatctgcgg cgttaccaat acagagacaa tatttaccaa taaaaaagtg tgagactaat
     9421 aacaatggtt tttagcatat gcctttgcca ttaagttgca taacgattta aatagtcaag
     9481 ttgcgcattc atttccttat tttttaactt ataagcctga gaaaaaagtt caggagggag
     9541 gtaaacatca taatgattgc cagtgtttgt taaataacca tactcaagat cttcatctgt
     9601 tgaaaaggat tcatttccca aggagattga tgccacatta ctattaacaa taaataatga
     9661 gcaaatgcta tctgaaagaa cagcaagaaa attcttcacc gctttaatcc tatccgacgt
     9721 gtcggtatct aaagcaagca aattcttgtt atcattaaat ataaactcgg gtaaatgcag
     9781 tatgcatttt ttgtcattca agcaactatc aatgaattgt tttttaaaaa acagaaagct
     9841 ccatgacata aaactaccca caccctgaat tttgttgtca gattgcagat caacgccaaa
     9901 taatgtcttt acatcttccc tgagattata gaatgccgta tcaacaagag cctcgatcgt
     9961 ttgtttatca acaattttaa taaactcttc gtatacttgc ttttgcgtct tatggctgcc
    10021 cagcgcccat gaatactcat gttcttttaa aaccagtatg gctcttaaca gacaatatcc
    10081 atcgccatct actggagcgt attttaatcc atatgtttta ttaatttcac tctgaataaa
    10141 agatatgcca gacaaatcaa taccagtatc taaatcagat attcccggca tatttatctg
    10201 ttctggctgg atactcatat tttcatcatc taaacaatac tcagaaaaaa catcatcaca
    10261 cgcattaatg ttttcaatat attccataaa gccataccaa taataacctt taaaatctgt
    10321 gataggctct gaacctctgc ccggcaagga cactctaaca atatcaacat cttgaacata
    10381 ttgtatagta tatgtaatac ctcctgaaac ccctgaataa atttgtgtat cattagtacg
    10441 caatgttctc ataaataatg atagatcatt atcattctgc atttcctcaa attctttttc
    10501 tagtgattca cattccagac atggagtatc ctcttcattt actttacgtc tgagttcatt
    10561 gtacaaccgc ataaaacaca ccagtgtgtt ttcaaaaagc gtgattgaat atgtttgatt
    10621 tttttcgtac tcatttttta tatcgttcca ggcctgtttc aaataattat ttgcagtgca
    10681 actaattcca tgtatattac tacccatata tagcgtctat aaaatttaat aaataatgac
    10741 gccctagtta aacttaaagt gcctggttca actatcaaaa atcgctcacc ctttttcacc
    10801 tgtttaaaat atgttcagca acccatcttg atggcgacct cctctccgcg atgatttcaa
    10861 taacatattc tgtgttggca tatgaaattt tgaggattac cctacactta taggagttac
    10921 cttacagggg ttccttcaat ttgtgttgat ttacgcgaga taacgctatg gaattaaagg
    10981 attattacgc catcatgggc gtgaaaccga cggacgatct caagacaatc aagaccgcct
    11041 atcgtcgact ggcccgtaaa taccatcctg atgtcagcaa agaaccggat gccgaagccc
    11101 gcttcaaaga ggtcgctgaa gcctgggaag tgttaagtga tgaacaacgt cgcgctgagt
    11161 atgatcagat gtggcaacat cgcaacgatc cgcaatttaa ccgtcagttc catcatggcg
    11221 acggtcagag ttttaatgcc gaagattttg acgatatctt ctcgtcaatt ttcggtcagc
    11281 atgcccgcca gagccgtcaa cgccccgccg cacgcggcca cgatattgaa atcgaagtgg
    11341 cggtattcct cgaagaaacg cttactgagc ataagcgtac catcagctat aacctgccgg
    11401 tttataacgc ctttggcatg atcgaacagg aaatcccgaa aacgctgaat gtgaagatcc
    11461 cggcaggcgt cggcaatggt caacgcatcc gcctgaaagg ccaggggacg ccgggcgaaa
    11521 acggcggtcc aaatggcgat ttgtggctgg tgattcatat tgcgccacat ccgctgttcg
    11581 atattgtcgg ccaggatctg gaaattgtgg tgccggttag cccgtgggaa gcggcgctgg
    11641 gtactaaagt caccgttcca acactgaaag aaagcatttt gctgactatc ccgccaggca
    11701 gccaggccgg gcaacgattg cgcgttaaag gcaaaggtct ggtgagcaaa aaacagaccg
    11761 gcgatctgta tgcggtactg aaaatcgtga tgccgccgaa accggatgaa aacactgccg
    11821 cgctgtggca gcaactggca gacgcccagt cgtcttttga tccacgtaaa gattggggga
    11881 aagcataatg gctaatgtta cggtgacttt tactattacc gaattttgcc tgcataccgg
    11941 catctctgaa gaggagttga atgaaattgt cggtttgggg gtggttgaac cgcgtgagat
    12001 ccaggaaaca acctgggtat ttgacgacca tgccgccatt gtggtgcaac gcgcggtacg
    12061 cctgcgtcat gaactggctc tggactggcc ggggatcgcg gtggcgctga cgttaatgga
    12121 tgatattgcg cacctgaagc aggaaaaccg cctgctacgc cagcggcttt cccggtttgt
    12181 agctcatccg tgatgtgtgt ttgccggatg tgatgacgca tccggcaact gcgccataat
    12241 gcctgatgcg acgctaaacg cgtcttttca ggcgcataaa cgacgttatc tgttttggtg
    12301 gtcgcactcc actaacacca gcaataactg gcttagtgcc gcgtaaaaac caaagctgtc
    12361 atactgtcga caacgcgcag caaactctgg taaccattgc cgcagcgccg tcagtgtttt
    12421 ttgccgcaaa ctgtcgattc ttcgcgcagg aacggtcccc tctcccagcg aaaaatgcag
    12481 ataactcaac aactcgagat agatcgccag atgatctgcc ggttcgttga aattgccact
    12541 ggtttccatc cctgcctcaa ctaacaagcg tttaatctct tgctcgtcct gtttgtaggc
    12601 cgatgcatac ggcagcgccg cttgtttgtc agtcatcaga aacaggccgc aaaagtcagc
    12661 ggccagttcc agacgggcat cgtcacgtac tgtcagcgt
//
LOCUS       DAJVRL010000103        12601 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVRL010000103 DAJVRL010000000
VERSION     DAJVRL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.103"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(76..795)
                     /locus_tag="OQN02_003171"
                     /pseudo
     CDS             complement(76..795)
                     /locus_tag="OQN02_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(811..1143)
                     /gene="cusF"
                     /locus_tag="OQN02_003172"
     CDS             complement(811..1143)
                     /gene="cusF"
                     /locus_tag="OQN02_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCS1643875.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1167..2549)
                     /gene="cusC"
                     /locus_tag="OQN02_003173"
                     /pseudo
     CDS             complement(1167..2549)
                     /gene="cusC"
                     /locus_tag="OQN02_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            2706..3389
                     /gene="cusR"
                     /locus_tag="OQN02_003174"
     CDS             2706..3389
                     /gene="cusR"
                     /locus_tag="OQN02_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS1643876.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3379..4821
                     /gene="cusS"
                     /locus_tag="OQN02_003175"
     CDS             3379..4821
                     /gene="cusS"
                     /locus_tag="OQN02_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS1643877.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            4971..7208
                     /gene="nrfB"
                     /locus_tag="OQN02_003176"
                     /pseudo
     CDS             4971..7208
                     /gene="nrfB"
                     /locus_tag="OQN02_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7195..10199
                     /gene="nfrA"
                     /locus_tag="OQN02_003177"
                     /pseudo
     CDS             7195..10199
                     /gene="nfrA"
                     /locus_tag="OQN02_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10200..11090
                     /locus_tag="OQN02_003178"
     CDS             10200..11090
                     /locus_tag="OQN02_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS1643878.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11273..12034
                     /gene="envY"
                     /locus_tag="OQN02_003179"
     CDS             11273..12034
                     /gene="envY"
                     /locus_tag="OQN02_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS1643879.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12080..12156)
                     /locus_tag="OQN02_003180"
     tRNA            complement(12080..12156)
                     /locus_tag="OQN02_003180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12120..12122),aa:Arg,seq:tct)
     gene            12399..>12601
                     /locus_tag="OQN02_003181"
     CDS             12399..>12601
                     /locus_tag="OQN02_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1643880.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3066 a         3281 c         3211 g         3043 t
ORIGIN      
        1 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
       61 acagataagc aacctacctt cctctttcgc gaccacgtta tctttggcga tattcattcc
      121 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt
      181 ctggattttt tgcgtggcga tcaggtggcg aatatccgcc tccggcattc ccgccagtcg
      241 cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcaacagtaa
      301 atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg gtgtgccctt
      361 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc
      421 ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa
      481 agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
      541 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
      601 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca tcgggtcgta
      661 ccagaataag attttacgtt ctgcggtcgg cattttttct gtgtgcggtt ccgcctggtc
      721 aaaccaggta aaacctgccg ctgaaataat accgcccgcg atcatgctgc cgataataag
      781 cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat cctgtaataa
      841 agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt taatttcact
      901 cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt tgacggcagc
      961 aatcggatcg tgatggatgg tgattttttt gctttccaga tcaacacctt ttaccacacc
     1021 ggtggcacta atgacctgtg gttgtgcttc gctcatggtt tcatgatgat gttcgttagc
     1081 ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca gtgctttttt
     1141 catttattct ctcctggagt taaaaattat tgctgccaac cgccccctaa ggcggtatac
     1201 aaataaattt cgttaacctg acgggcataa ttcaggtcaa gtaaagtttg tcgggttgca
     1261 aataaagaac gctcggcatc cagcacttcc agataactta ccgcgccgtg ctgatataat
     1321 gcccgcgccc gttgcagggt aatttgcagc gacgccagat aacgctgctg ggcgctgatt
     1381 tgatcgtcca ggctttgacg taatgccagc gcatctgcca cctctttaaa ggcgttctgg
     1441 attttctgtt cataattcac caccgactgc tgctggcgaa tttcggcgat atccagattg
     1501 gcctggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa attccacatc
     1561 ccgctgctag cgttaaataa tgacgacaga tcgctgctgg cggttgatat tccgctggtc
     1621 aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa tattagcatt agccgccatt
     1681 aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga cgacaagccc
     1741 gccggtaatt taacgctttg caggctgccg ctgtttactg tctgcgcttg cggtagcttg
     1801 ccgtagcttc ccaataacag ttgcaatgca ttattcgcct gcgccagttc cccctgacgt
     1861 ttagcgatgt cactgcgggt actttctatc accccgcgag cctgttccag cgccagaaca
     1921 ttgctgctac cggtcaacag ttgtttttcg acaaacgcat atgactgctg ataattacgc
     1981 agcgtttctt cggctatttg caattgcgca tacgccagct gctgattgaa atagctttgc
     2041 gcgacattag aaaccagcaa aatatgcacc gcgcgctggg cttcctcagt ggctaaataa
     2101 ttttggcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag atcaaagctg
     2161 gcgttaaggc cagtcgagaa ctcccgcgtc gtggctgaat cgcctttaag attgccgctc
     2221 cagctgccgc tgccctagcc attgagctgt gggtagcggt cggcatcggt cagacgatat
     2281 tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt attcaccagc
     2341 gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca gcccacgttc
     2401 tggtagttat ccgctgcgtt aaccaggccg ttctggctga gtgagaactg ctgcggtacg
     2461 ggcattaccg gacgctgata atccggtgcc agtgaacaac cagttagcgc aagggccaca
     2521 caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat tttatgtcgc
     2581 caactttact cgccaggctc tgattttccg gtgacaggaa aatgacaaaa ttgtcatttt
     2641 gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca acatgcggag
     2701 gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag
     2761 ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat ggctaccatc
     2821 tggcgatgac cggtgattat gacctgataa tcctcgatat tatgctgcca gacgtgaacg
     2881 gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt ctgttgctta
     2941 ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggca gatgactacc
     3001 tggtgaaacc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc ctgcggcgcg
     3061 gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc gatctcgtca
     3121 gccgcaaagt caccctcagc ggcacgcgca tcactctgac cagtaaagaa tttactctgc
     3181 tggagttttt tctccgccat cagggcgaag tgctgccccg ctcgcttatc gcctcgcagg
     3241 tatgggacat gaatttcgac agcgacacta acgccattga tgtggcggtg aagcggctac
     3301 gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc ggcgtgggtt
     3361 acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc gctggcaacc
     3421 cgcctgacct tttttatcag cctggccacc atcgcggcgt ttttcgcctt tgcatggatc
     3481 ataatccact cagtaaaagt gcattttgcc gagcaggaca ttaatgattt aaaagagatt
     3541 agcgccaccc ttgaacgggt actaaatcac cctgacgaaa cgcaagcccg acgcttaatg
     3601 acgctggaag atatcgtcag tggttattcc aacgtgttga tttccctggc agatagccac
     3661 ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc acgtgatgcc
     3721 atacccgata aagacgcgcg gggtggcgag gtgtatctcc tttccggccc gacgattatg
     3781 atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat taacttgccg
     3841 gttggcccgt tggtggacgg caaaccgatt tatacgctct acatcgcgct gtcgatcgat
     3901 tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc atcggtaatc
     3961 agcatcctga tcgtctttat cgtgctgttg gcggtacata aaggtcacgc gccgatccgc
     4021 agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgttcg ccttgacccg
     4081 cagaccgtgc cgattgagct ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt
     4141 attgaggatg tctttactcg ccagtccaat ttctcagcgg atatcgccca cgaaattcgc
     4201 acaccaatta ctaatctcat tacgcaaacg gaaatcgccc tcagccagtc gcgcagccag
     4261 aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg
     4321 gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc cgaaaagaaa
     4381 atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc gctagcggaa
     4441 gatcgcggcg tggagttgcg gtttgttggc gacaaacgtc aggttgcggg cgatccgttg
     4501 atgctgcgtc gggcgctaag caacctgctt tctaatgccc ttcgttatac gccaaccaga
     4561 gagacaattg tagtacgctg tcagacggtc gatcaccagg tgcaagtttc cgtcgaaaac
     4621 cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt ctatcgcgtt
     4681 gacccctccc gccagcgaaa aggtgaaggt agcggtattg ggctggcgat agtgaaatcg
     4741 attgttgtcg cgcataaagg cacggttgcg gtaacgtcag atgcgcgggg gacaaggttt
     4801 gttatcacat tacccgctta attattcagc aaacggcaac ttttataacc agtgtaaaaa
     4861 taacgtgccg caatagtcat acatattaat ggtaaaaagc tgtcacaatt cataaaaaac
     4921 cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt gtggactggc
     4981 ttcttgatgt ttttgctacc tggctctacg gcttaaaagt aatcgcgata acgttagcgg
     5041 tcatcatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac tgggtacgcc
     5101 gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac cgcgaactgt
     5161 ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa acgggcgtca
     5221 tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat catatctttg
     5281 ttggcaccta ccccaacgac cccgatactc aacgtgatgt tgacgaagtg tgcgctcgct
     5341 ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa gccgactgcc
     5401 tgaacaacgt gctggacgcc atcacccagt ttgaacgtag cgccaatttc gcttttgctg
     5461 gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt ctgttcaact
     5521 atctggtcga gcgtaaagat ctgattcaga tcccagtgta tccgtttgaa cgcgaatgga
     5581 cgcacttcac cagcatgact tacattgatg agttttcaga actgcatggc aaagatgttc
     5641 cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc tgtttcagcc
     5701 gccgcgccgt gaccgcactg ttagctgacg gtaacggtat tgctttcgac gtgcagagtc
     5761 ttactgaaga ttacgacatt ggcttccgcc tgaaagaaaa aggtatgacg gaaatttttg
     5821 tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt ttacagcacg
     5881 cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt tcgactgcgg
     5941 ttcgacaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa acccataaat
     6001 ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg gcaatcagta
     6061 actttgtcag cttcctcgcg atgctggtga tgatccagct tttgctgttg ctggcgtatg
     6121 aaagtttgtg gcccgatgcc tagcatttcc tttctatttt tagcggcagc gcatggttaa
     6181 tgaccctgct gtggctaaac tttggcttga tggttaaccg catcgtgcag cgagtgattt
     6241 tcgtcactgg ctactacggc ctcacgcagg gcctgctttc cgtcctgcgt cttttctggg
     6301 gcaacctgat taactttatg gctaactggc gcgtgctgaa acaggttctt cagcacggcg
     6361 atccgcgtcg cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtc actggcgata
     6421 cccgctcgtt gcgcccgtta ggtcaaattc tgctggaaaa tcaggtcatc actgaagaac
     6481 aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt tcaatgctga
     6541 tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa aacggcgtgg
     6601 cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct gattgccgaa atgccagcct
     6661 ccgtagcgct gcattatgca gtactgccgc tgcgtctgga aaatgatgag ttaattgtcg
     6721 gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa gtcggacgca
     6781 aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac agggttacgc cactggtatg
     6841 cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag catcagtggc
     6901 tcacggaaca gcagaccggt gaaatctggc ggcaatatgt gccgcatcag ttcctgttcg
     6961 ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg ttgttattgc
     7021 gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc gttatcagcc
     7081 aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg atgcaatcac
     7141 tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc gaaaatgaag
     7201 gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc tttattgagt
     7261 accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct gagcgattat
     7321 cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa aaataacgac
     7381 gaagcaaccg ccatccgcga atttgaatat atacaccaac aggtgccgga taatattccg
     7441 ctgactttat accttgcgga agcctatcgt cattttggtc atgatgaccg ggcgcggctg
     7501 ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg cagtctggcg
     7561 gctattccag ttgaagtgaa aagcgttacg actgttgaag aactgcttgc ccagcaaaaa
     7621 gcgggcgacg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa taccctgcgg
     7681 ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc tgcatcgccg
     7741 gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa tctacctgaa acaatgatcc
     7801 caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc ggcagaacgc
     7861 cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct ggcactgcaa
     7921 tcacagggga tcttcactga tcctcagtca tatattactt acgcgaccgc gctggcttat
     7981 cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa ataagccgct gtttaccacg
     8041 ggcgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa ccccgttcag
     8101 gcattggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt tggcgcgacg
     8161 ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct cgccaccctc
     8221 cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg taacaaggct
     8281 gaatctctgc gtctggcacg attgctgtat cagcaagaac cgacaaatct tccccgcctg
     8341 gatcaactga cctggcaact gatgcagaac gagcagtcac gcgaagctgc cgatttattg
     8401 ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agactttaat ggcgcgactg
     8461 gcgtctctgc tggaaagtca tccttaccta gcaacgccgg cgaaggtggc gattttatcg
     8521 aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg tattgcagat
     8581 aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga tgctgccgcc
     8641 tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgtt gtatgcatgg
     8701 cttcaggccg aacaacgaca accgagcgcc tggcaacatc gtgcggtagc ctatcaggcg
     8761 tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag tcttcacgac
     8821 atgagtaatg atgatctgct cgccgccgcc aacaccgctc aggcagcggg aaatggcgcg
     8881 gctcgcgatc gctggcttca acaggcagaa caacgtgagc tgggaaacaa tgctcaatac
     8941 tggtggctgc atgcgcgacg ttatatccca gctcagccag aactcgcact gaacgatctc
     9001 acgcgctcaa tcaatattgc cccttctgcc aactcttacg ttgcgcgggc gacaatttat
     9061 cgccaacgtc ataatgttcc ggccgcggtg agtgatttgc gcgccgcgct ggaactggaa
     9121 ccaaataata gcaacaccca ggcagcgctt ggttacgcct tgtgggatag cggtgatatc
     9181 gcacagtcgc gggaaatgct cgaacaggcg cataaagggc ttccggacga tccggcactg
     9241 atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac gcagcactac
     9301 gcccggctcg tgattgatga cattgataat caggcataac cccactgacc ccagagcaaa
     9361 atcagcaacg cttcaatttc cgccgtttgc atgaggaggt cggtcgccgc tggacgttca
     9421 gtttcgattc ttccatcggc ttgcgttccg gcgcaatgag taccgctaac aataatgtcg
     9481 gcggtgcagc gcctgggaaa agctatcgta gctacggaca actggaagcc gagtaccgca
     9541 tcggacgcaa tatgctgctg gaaggcgagt accgcatcgg acgcaatatg ctgctggaag
     9601 gcgacctgct ctcagtttat agccgcgtct ttgccgatac cggagaaaac ggggtgatga
     9661 tgccggtgaa aaatccgatg tccggcaccg gtctgcgctg gaagccgctg cgcgatcaga
     9721 tctttttcct cgccgtcgaa cagcagttgc cgctgaacgg gcaaaatggc gcatccgata
     9781 ccatgctgcg cgccagcgcc tcattcttta atggcggcaa atacagcgac gaatggcacc
     9841 cgaacggttc aggctggttt gcccaaaacc tgtacctcga tgcggcgcaa tatatccgcc
     9901 aggatattca ggcgtggacg gcagattatc gcgtcagctg gcatcagaag gtagctaacg
     9961 gacagactat tgagccttac gctcacgttc aggacaacgg ctatcgtgat aaaggcactc
    10021 agggcgcgca gcttggcggt gtcggggtcc gctggaatat ttggaccggc gagacgcact
    10081 acgacgcctg gccgcacaaa gtcagtctcg gcgtcgagta tcaacatacc tttaaggcga
    10141 ttaatcaacg taacggagag cgcaacaacg cgtttctcac cattggagtg cactggtaaa
    10201 tgcgtaagtt cattttcgta ttgctgacac tgcttttggt cagccctttt tcctttgcga
    10261 tgaaaggtat tatctggcaa ccacaaaacc gagatagtca ggttaccgat acccagtggc
    10321 aggggctgat gagtcagtta cgttttcaag gcttcgatac cctggttttg caatggaccc
    10381 gttacggcga tgcatttacc cagccagaac agcgcacgtt attgtttaag cgtgccgcag
    10441 ctgcgcaaca gactggcctg aagctgattg tcgggctgaa cgccgatccg gaatttttta
    10501 tgcaccagaa acagtcgtct gcagcgctgg aaagctatct taatcgcctg ctggctgccg
    10561 atctccagca agccagatta tggagcgccg cgcctggcat aacgccggat ggctggtaca
    10621 tcagcgcgga aattgacgac ctgaactggc gcagcgaagc cgcccgtcaa cctttgctaa
    10681 catggttaaa caacgcgcag cggctgatta gcgatgtttc agcaaaaccg gtttatatca
    10741 gtagtttttt cgccggaaac atgtcgcccg atggctatcg ccaactgctg gaacaagtta
    10801 aagcaaccgg agttaatgtc tgggtacagg atggcagcgg cgtggataaa cttaccgctg
    10861 aacagcgtga acgttatcta caggccagcg ccgattgcca aagctccgcc ccagcaagcg
    10921 gcattgttta tgaactgttt gtcgccggca aaggcaaaac ctttacagcg aaaccgaaac
    10981 cggacgcaga aattgcctcg ctgttagcga aacgttcctc ttgcggtaaa gacatgctct
    11041 atttctctct gcgctatttg cccgtcgcgc acggcattct cgagtattaa atcccctcca
    11101 ggtaagtcgg gtacgacctg gcttacctct ttcgctctcg tcagaattcc atcaagatta
    11161 attgctaaaa agcatgctaa agaaagaaga tcgttatcgg cgaacgggcc gatcactcat
    11221 gcaggaagca tagatccttt gagcggagtt tctgatatct gaggagtgcg aaatgcaatt
    11281 gagcagcagt gaaccttgcg tggtgatcct gaccgaaaaa gaggtagagg taagcgtcaa
    11341 taaccatgct acgtttaccc ttccgaaaaa ctacctggcc gccttcgcgt gcaacaataa
    11401 cgtcattgaa ctctcaacgt taaatcacgt attaatcacc cacatcaacc gtaacatcat
    11461 caacgattat ctgttgtttt taaataagaa attaacctgt gtaaagccct ggtcgcggct
    11521 ggcaaccccg gttatcgctt gtcatagccg tacaccggaa gtgttccggc tagccaccaa
    11581 ccacagcaag cagcaatcca gcaaaccctg cgaagcggag ttgacgcgcg cattactttt
    11641 taccgtattg tctaactttc ttgagcaatc gcggtttatt gccctactga tgtatatctt
    11701 acgcagcagc gtccgcgaca gcgtctgccg cattattcaa agcgatattc agcattactg
    11761 gaatctgcga attgtcgcca gttcgctatg tttaagcccc agcctgctca aaaagaaatt
    11821 aaaaaacgaa aataccagct atagccagat tgtcacagag tgtcgtatgc gttacgccgt
    11881 acagatgtta ttgatggata acaaaaatat cactcaggtg gcgcaattat gtggctatag
    11941 cagcacgtcg tactttatct ctgtttttaa ggcgttttac agcctgacac cgttgaatta
    12001 tctcgccaaa cagcgagaaa aagtgatgtg gtgaagggca aagcggaaac ggataagacg
    12061 ggcataaatg aagaagaaat ggcgcgccct gcaggattcg aacctgcggc ccacgactta
    12121 gaaggtcgtt gctctatcca actgagctaa gggcgcgttg ataccgcaat gcggtgtaat
    12181 cgcgtgaatt atacggtcaa cccttgctga gtcaatggct tttgatctgg ttgctgaaca
    12241 agtgaacgac cgcgtctgat tttctgattt atttcgctat agcggcaaac aaacgcacac
    12301 cgctgcgcgt ctgaatcaag aaaacccgta ttttcatgta tcaaagtaca atttaccgcc
    12361 ctaacggaaa attgtccgct cctatgagac tggtaactat gaaaccaacg tcggtgatca
    12421 ttatggatac tcatcctatc atcagaatgt ctattgaagt tctgttgcaa aaaaacagtg
    12481 aattgcagat tgtcctgaaa acggatgatt accgcataac catcgattat ctccgaaccc
    12541 gtcctgttga tttaatcatt atggatatag acttgcccgg aacagacggt tttaccttcc
    12601 t
//
LOCUS       DAJVRL010000104        12414 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVRL010000104 DAJVRL010000000
VERSION     DAJVRL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.104"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..1199
                     /gene="edd"
                     /locus_tag="OQN02_003182"
     CDS             <1..1199
                     /gene="edd"
                     /locus_tag="OQN02_003182"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1643881.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1236..1877
                     /gene="kdgA"
                     /locus_tag="OQN02_003183"
                     /gene_synonym="eda"
     CDS             1236..1877
                     /gene="kdgA"
                     /locus_tag="OQN02_003183"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1643882.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OQN02_003184"
     CDS             complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OQN02_003184"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1643883.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3245..3535
                     /gene="yebG"
                     /locus_tag="OQN02_003185"
                     /pseudo
     CDS             3245..3535
                     /gene="yebG"
                     /locus_tag="OQN02_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3602..3958
                     /locus_tag="OQN02_003186"
     CDS             3602..3958
                     /locus_tag="OQN02_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1643884.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4285..4944
                     /locus_tag="OQN02_003187"
     CDS             4285..4944
                     /locus_tag="OQN02_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1643885.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5154..7214
                     /gene="ptrB"
                     /locus_tag="OQN02_003188"
     CDS             5154..7214
                     /gene="ptrB"
                     /locus_tag="OQN02_003188"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS1643886.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OQN02_003189"
     CDS             complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OQN02_003189"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1643887.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIYRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7897..8553)
                     /locus_tag="OQN02_003190"
                     /pseudo
     CDS             complement(7897..8553)
                     /locus_tag="OQN02_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OQN02_003191"
     CDS             complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OQN02_003191"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1643888.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9024..9398
                     /gene="yobA"
                     /locus_tag="OQN02_003192"
     CDS             9024..9398
                     /gene="yobA"
                     /locus_tag="OQN02_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1643889.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9402..10274
                     /gene="copD"
                     /locus_tag="OQN02_003193"
                     /pseudo
     CDS             9402..10274
                     /gene="copD"
                     /locus_tag="OQN02_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10287..10628
                     /locus_tag="OQN02_003194"
     CDS             10287..10628
                     /locus_tag="OQN02_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1643890.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11021..12097)
                     /locus_tag="OQN02_003195"
                     /pseudo
     CDS             complement(11021..12097)
                     /locus_tag="OQN02_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12063..12344)
                     /locus_tag="OQN02_003196"
     CDS             complement(12063..12344)
                     /locus_tag="OQN02_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCS1643891.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3080 a         3032 c         3136 g         3166 t
ORIGIN      
        1 tggccttact ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg
       61 gtactgccaa caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt
      121 cttttgttca tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg
      181 ttacacgcat gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga
      241 aagtggtggt gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca
      301 tgcacctggt ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg
      361 acctttctga tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta
      421 accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc
      481 tgctgcatga agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac
      541 catggctgaa taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca
      601 atgtgatcgc ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg
      661 gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg
      721 aagcgccagc ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt
      781 tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc
      841 cagaattaca taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg
      901 cgttagttac cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg
      961 taacaccaga agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc
     1021 gtgtgaatgg acagacaggc gaactgacac tgctggtaga cgaagcggaa ctggctgctc
     1081 gcgaaccgca cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca
     1141 gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag
     1201 acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag
     1261 aatcaatcct gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg
     1321 cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc
     1381 gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg
     1441 tgggtgccgg tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac
     1501 agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta
     1561 ttcctctgat tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt
     1621 tgaaagagtt caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga
     1681 tcgcgggtcc gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact
     1741 accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg
     1801 cagatgcgct ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag
     1861 aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt
     1921 tttttgccgg gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat
     1981 ggcgtcaaca acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat
     2041 ttccggctta ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc
     2101 aaacgtgaca ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata
     2161 ctgacggatc ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga
     2221 gagatcttga gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact
     2281 gaaaatcacc tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag
     2341 tgccagcacc actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg
     2401 ctgtggttgc caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca
     2461 gaaatggacg ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac
     2521 gacgccttca acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca
     2581 tgcctgagca agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat
     2641 caccggtttt acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttt
     2701 gctatcggca aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg
     2761 gataccctcg cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc
     2821 ttcctcttca agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata
     2881 atgtggtttt tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac
     2941 atgggagcga tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac
     3001 ctctacgccg agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa
     3061 taacatcacg cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat
     3121 cccctgatta attccgctcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga
     3181 tgttgatttc tcaaccgaaa agaaatatac tgtataaaat ctcagttatt atgagaggct
     3241 gcaaatggcg gttgaagttt aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt
     3301 taccagcaaa aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga
     3361 cacctagctg acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct
     3421 atggctggca gaacaaaaag aggtgctgag caccattctg aaaaccggca aattaccgtc
     3481 cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa
     3541 cttgcgctcc tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa
     3601 catgaaaaaa agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt
     3661 cgctgccaat aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc
     3721 tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc
     3781 agatgatcaa aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat
     3841 tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat
     3901 tcattaccag gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg
     3961 gcttctgata attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgtcccaga
     4021 acgcaccaca atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca
     4081 aacaatgcca ctcaaaacga caaactactt tcacctaaaa tacccttatc acttgatata
     4141 cagtaaatac cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt
     4201 gcgcatcttt tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact
     4261 aaaaatggtt attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt
     4321 gggcaaagca gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg
     4381 tcaggcgcat taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt
     4441 cttacgaaat atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg
     4501 ctgtggaata aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc
     4561 gcgcaaagta cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc
     4621 gctaaaagtg atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt
     4681 gaagccggcg tggaagagca ggggcgtgta ctcattgagc aggcaatcga acaaccgctg
     4741 gatccacaac gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc
     4801 ctgagctgcg cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg
     4861 ggtgacgcgc tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag
     4921 caaaaacgca ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg
     4981 ggctatccag aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc
     5041 gatatccgcg ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc
     5101 ccggtgagtt ttgccaccct tataagatgt ttccaaccag aaagaacaat aacatgctac
     5161 caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc atcgataatt
     5221 actactggct gcgggacgat acgcgttctc agccaaaagt cctggactac ctgcaacaag
     5281 aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat cgcatcttaa
     5341 aggaaatcat cgaccgcatt ccgcaaagag aagtttctgc gccctacata aaaaatggct
     5401 accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag cgtcaatcgg
     5461 cattcagcga agagtgggat gagtgggaaa cattgctcga tgccaacaag cgcgcagctc
     5521 atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc attatggcgc
     5581 tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat ctggaaacag
     5641 gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg gcaaatgact
     5701 cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat caggtctggc
     5761 gtcacgccat cggtacgcca gcatcgcaag atacactgat ctacgaagaa aaagacgata
     5821 cctattatgt cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt catctggcca
     5881 gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc gagccgtttg
     5941 tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag catcgttttt
     6001 atctgcgttc caatcgccac ggcaaaaact ttggcttata ccgtacccgt atgcgtgatg
     6061 agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa gggtttacgc
     6121 tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt ttgcgccaaa
     6181 ttaaccgcaa gacccgggaa gtcattggca ttgcctttga tgatccggcc tatgtgacct
     6241 ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat tcttccatga
     6301 ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt gtattaaaac
     6361 aaacagaagt gcctggtttt gatgcggcga attaccgcag tgaacacctg tggatagtcg
     6421 cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat tttcgcaaag
     6481 gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt gatgccgatt
     6541 tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt gtccatgttc
     6601 gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg aagaagaaaa
     6661 atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc tatggctctc
     6721 cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc gttgcaatta
     6781 atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt gatgttgtaa
     6841 caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag tggggaaatc
     6901 cgcaggatcc gcaatattac gagtacatga aaagctacag cccgtatgac aacgtcaccg
     6961 cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag gtgcaatatt
     7021 gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac catcttttat
     7081 tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt aaatcgtacg
     7141 aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta
     7201 cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca
     7261 tgctgttcag gttattaaat aaccagcgca gatagcccgg atagcgttcg gcaacgtcgg
     7321 aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg
     7381 tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg
     7441 cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggtgtctgta
     7501 cattgagctt gcgtgtttta tataacgcca tattgctgta cttgagccct ggccacaaac
     7561 gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt
     7621 caaagctggc gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt
     7681 caatccacgg tttatcggcg accatggctt cggtgatgcg ataaatcgcc atcgcttgtg
     7741 gactaatagg acgatcgggg cgcaccagga ggctcatggg gttgacgatt tttccgtcaa
     7801 tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga
     7861 taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc
     7921 ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac
     7981 gatgagacga ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac
     8041 ggcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg
     8101 ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat ccataccttg
     8161 cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact
     8221 ctgatggtag ttcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa
     8281 gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgctagg tcgttgcggc
     8341 ataacaaagc gggtctaaca gtgaaagatc gggtggggca ggaagggcac gtcgcgaata
     8401 atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc
     8461 ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc
     8521 atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca
     8581 aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta
     8641 actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag attgaccgaa
     8701 gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc tggctgttca
     8761 cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaaagc caccccggcc
     8821 gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc
     8881 agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc
     8941 aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt
     9001 tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg
     9061 caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc
     9121 agtatcctgc ggcaaacgcg caagtgacag ctgccccgca ggcaatcact ttaaacttct
     9181 cggaaggcgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata
     9241 ttaaaacatt accagcgaag cggaatgagc aggaccaaaa gcaactgatt gttccactgg
     9301 ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc
     9361 ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga
     9421 tcgcgctgcg atttatccat ttcacctcgt tgatgctggt ctttggcttt gcaatgtatg
     9481 gtgcctggct ggccccctta atgattcgtc gcttgctgac gaagcgttct ctgcgtttac
     9541 aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg
     9601 ggttgatggg aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc
     9661 aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg
     9721 tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat
     9781 ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaaca gcgaaaatcc
     9841 atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc
     9901 cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acactaggct attcaggcgc
     9961 tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc
    10021 ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct
    10081 tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg
    10141 ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga
    10201 ctaaactcga atgggcgata ggtgcggtag tgctggtgat catcagcctg cttgcgactc
    10261 tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac
    10321 tcaccagttc tgcagcggcg ctggcggctc cacaggtgat aaccgtgagt cgttttgaag
    10381 tgggtaaaga caaatgggcg tttaatcgcg aagaggtaat gctgacttgc cgaccgggta
    10441 atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatatcgcac
    10501 aaaaggaagt tgccagtggg aagaccaatg cccaacccat ttcggtgatt cagattgatg
    10561 atcctaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac
    10621 tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc
    10681 accagaacgg gcggttttta acatttaagc tgatgagcac cgcgcttttt attgaccatt
    10741 ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag
    10801 cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg
    10861 aatacaggaa tcgtattcgg tcttttttta attgtattta aaatcaatta gttgcaaacg
    10921 tctccccgaa attccccgaa atttactcga atttctgtat tccggtcttt tttggttata
    10981 tcacaaccaa aatacattta acaatccatt tacgttaaaa tcagagcagt aagtacgttt
    11041 tttctctctc atcaagatac atttttgttg tcttctccga tgtgtggcca agtagacgct
    11101 gagcaaattc ttctccacat atttctttgt acaatcgtcc agccaggctt ctgatttcgt
    11161 gaaaagttgg tgggttttca ctgaacagga tacctgttaa ttttctggct gcgacaaatt
    11221 tctttgtcag gccgtccggg tgaatgctgc cgtcagggct gttttttcta atcccggcac
    11281 tgattagata atccccccta cttaccatgc ggcactgttc aactactgca ccaagtcgta
    11341 aaccagcgac aggaaggctg agtgacaggg ggatagcaat catcattcct gtcttaattt
    11401 gcctgatgtg gagacgatca tcataaatat cactgaaccg catattcgtt atgtcttcgc
    11461 gacgttgtcc tgttacaagg gctaagtcca tagctaatgg gaaccatgcc ggaagttgat
    11521 ctgctgcctc cctgatgcag ttgtatgtct ttagtttcag ccgttctctt gtaactacta
    11581 ttttcggtgc tcttgttggc gttactggat tttgagatat acgtccttca acaatggctt
    11641 cgcggaacat atcagataac acagaacgca ttgatcctgc catcgtgttt ttccctcctt
    11701 caatctacaa atcaagaaac tcggcgatat ggcgagtggt tatttctgtc agtaaaaccc
    11761 ctcctaattt ttcttttatt gtctccagtt gatttacccg agttttataa gtatttctgg
    11821 acgcttttct ccttataaga atcgttttgt aacgttcaat ccagcctgcc atagtaaatg
    11881 agtcgacccc tttaagcttt tcaattaggg cagcaggaga gtagtttttg tatatataat
    11941 gatttgcttc aattgcctgc gctactgcat ctcttcttga aattttacct agtgtaaatt
    12001 cttctttcgt cagagggttg cgccagtaat atgctttgtc tctccttcga tatgttaagt
    12061 ttctaggcaa attgggatcg tattttttcc gctgcatgtt ttaacttctc cattaacgga
    12121 ctgtctctcc cttgtcgccc attaggctga tggtgtgtta tatcggtatc aaccttattt
    12181 gggttgatat agaaagcctc cggaaccacc ctgtaactcc tcccgtgtag ttcaggtgca
    12241 ggataaatgt ttccattcct tgcccatcgt ctcagcgttg atattgatgg tgggttatcc
    12301 ggatatctga gttttcccca cgttttgagt gtcacaagat tcattgccat acctctcacg
    12361 atatgaccgc cagtaaatat acagaatact ggcgggtgtg gttgattttt aata
//
LOCUS       DAJVRL010000105        12209 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVRL010000105 DAJVRL010000000
VERSION     DAJVRL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.105"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..482)
                     /locus_tag="OQN02_003197"
     CDS             complement(<1..482)
                     /locus_tag="OQN02_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1643892.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     "
     gene            complement(513..1205)
                     /gene="fimC"
                     /locus_tag="OQN02_003198"
     CDS             complement(513..1205)
                     /gene="fimC"
                     /locus_tag="OQN02_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1643893.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1425..1967)
                     /gene="fimA"
                     /locus_tag="OQN02_003199"
     CDS             complement(1425..1967)
                     /gene="fimA"
                     /locus_tag="OQN02_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1643894.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEMLDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2448..3314
                     /gene="folD"
                     /locus_tag="OQN02_003200"
     CDS             2448..3314
                     /gene="folD"
                     /locus_tag="OQN02_003200"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1643895.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3316..3528
                     /gene="ybcJ"
                     /locus_tag="OQN02_003201"
     CDS             3316..3528
                     /gene="ybcJ"
                     /locus_tag="OQN02_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1643896.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3636..4145
                     /locus_tag="OQN02_003202"
     CDS             3636..4145
                     /locus_tag="OQN02_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1643897.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4181..5566)
                     /gene="cysS"
                     /locus_tag="OQN02_003203"
     CDS             complement(4181..5566)
                     /gene="cysS"
                     /locus_tag="OQN02_003203"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1643898.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5740..6234
                     /gene="ppiB"
                     /locus_tag="OQN02_003204"
     CDS             5740..6234
                     /gene="ppiB"
                     /locus_tag="OQN02_003204"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1643899.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6237..6959
                     /gene="lpxH"
                     /locus_tag="OQN02_003205"
     CDS             6237..6959
                     /gene="lpxH"
                     /locus_tag="OQN02_003205"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1643900.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7077..7586
                     /gene="purE"
                     /locus_tag="OQN02_003206"
     CDS             7077..7586
                     /gene="purE"
                     /locus_tag="OQN02_003206"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1643901.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7583..8650
                     /gene="purK"
                     /locus_tag="OQN02_003207"
     CDS             7583..8650
                     /gene="purK"
                     /locus_tag="OQN02_003207"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1643902.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8787..9680)
                     /locus_tag="OQN02_003208"
     CDS             complement(8787..9680)
                     /locus_tag="OQN02_003208"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1643903.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9677..10324)
                     /locus_tag="OQN02_003209"
                     /pseudo
     CDS             complement(9677..10324)
                     /locus_tag="OQN02_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10327..11559
                     /gene="allC"
                     /locus_tag="OQN02_003210"
     CDS             10327..11559
                     /gene="allC"
                     /locus_tag="OQN02_003210"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCS1643904.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11570..>12209
                     /gene="allE"
                     /locus_tag="OQN02_003211"
     CDS             11570..>12209
                     /gene="allE"
                     /locus_tag="OQN02_003211"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCS1643905.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2967 a         3152 c         3114 g         2976 t
ORIGIN      
        1 aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc aaagttaatt
       61 gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg ctgggctaac
      121 tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt aaaacagggc
      181 attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac gatatcctgt
      241 gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc aggttgatga
      301 ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa taaaaatgcc
      361 gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag agatgaataa
      421 caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt gggtattttc
      481 ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc tctgaccggg
      541 gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac tcctgagggt
      601 aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt ttggctgccc
      661 acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt gatatagtta
      721 ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg taattgaatt
      781 ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt cctgccgttc
      841 aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag agactcacga
      901 tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct tttgggttca
      961 ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct gctgttttca
     1021 atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca taccgcagtc
     1081 tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc tccggcagca
     1141 tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt tattttagtc
     1201 atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat aaagaaatga
     1261 ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg catgatacaa
     1321 agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg gtacattact
     1381 ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc gtatctcata
     1441 ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt gcctttataa
     1501 cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt tgagaagcta
     1561 ttcccatcag gtttgagaat tgcggatgta ttatcaagca tttcaatacc gacacccgtt
     1621 gctgttgttg tatttgtact ggatgcaatg gccaataaat tatcattgat tgcatcagcc
     1681 tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc gttcaactga
     1741 atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc ggtacgatat
     1801 tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc agcattcacc
     1861 agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact acccgtagcg
     1921 gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata tattccttca
     1981 atctcattta tcgactccac atccgtatat aaccgattac tttatttaag acactgatag
     2041 tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca ctctattcta
     2101 aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcaa cacaaatata
     2161 gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg tatttcttaa
     2221 attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg ccatcatgta
     2281 cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac cttcgcaaga
     2341 ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct tctgacaaaa
     2401 tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg gcagcaaaga
     2461 ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa aaagttcagg
     2521 ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg ggtagtaacc
     2581 ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc gggttcgtct
     2641 cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag cttatcgatg
     2701 cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta ccggcgggta
     2761 ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac ggtttccatc
     2821 cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc accccgcgcg
     2881 gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac gccgtggtga
     2941 ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc gcaggttgca
     3001 ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa aatgccgatc
     3061 tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc aaagaaggcg
     3121 caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg ggcgacgtcg
     3181 tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc ggcgttggcc
     3241 cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa tatcatgatc
     3301 cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt tgagctgtgc
     3361 gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat cgcgattgcc
     3421 gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa aatcgtcgcc
     3481 ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc cctttcccgg
     3541 cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc gttcatccat
     3601 gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt acgcacgccg
     3661 ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt gccggaatca
     3721 tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt gcttacggca
     3781 atgtttttgg tcatcgcggt tttactcatt cgctggtgtt tgcgtttgtt gtcccgctct
     3841 tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc tggctgtttt
     3901 taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc ggtaagggcg
     3961 ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag
     4021 tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg attatttccg
     4081 aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac
     4141 gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga cgccaggtgg
     4201 tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca cgtgccgcat
     4261 ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa
     4321 tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt
     4381 gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg
     4441 ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac agcacggaat
     4501 aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt
     4561 cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag aggcgctcca
     4621 gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc
     4681 ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca tcgcgcacgg
     4741 taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt
     4801 gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg atttcgttct
     4861 cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg ttacccagct
     4921 gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg ccccacggag
     4981 acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc
     5041 gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga tcctgacgcg
     5101 acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgcca
     5161 cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt
     5221 gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca
     5281 tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca ttggcgcgtt
     5341 tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga
     5401 aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac
     5461 agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg tgaataggct
     5521 taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt
     5581 agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata
     5641 cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa
     5701 ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat
     5761 cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc
     5821 ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc
     5881 tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg
     5941 atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc aatggcacgt
     6001 actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc
     6061 ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt
     6121 gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa cggtatgcac
     6181 caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg
     6241 cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt
     6301 ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt cttggcgatc
     6361 tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag atggcggcag
     6421 cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc aaccgtgatt
     6481 ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg gaagaaaagg
     6541 tgctcgaact ttatggtcgc cgggtgttaa ttatgcatgg cgacacgctg tgcaccgatg
     6601 acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc
     6661 tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg aacagcaaag
     6721 aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct gtggtcagtg
     6781 cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc ccggcggtgc
     6841 atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc tggcatacgg
     6901 aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt ccgttttaaa
     6961 aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg
     7021 ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt
     7081 cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta
     7141 ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg
     7201 tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg
     7261 gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc atgattgccg
     7321 ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg
     7381 atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg
     7441 gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata
     7501 aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa gtgctggaaa
     7561 acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg
     7621 ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg tcgggctgga
     7681 cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg
     7741 gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga accgcgatgt
     7801 gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc tccacctgcc
     7861 gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt
     7921 aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggag
     7981 tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat gtattgtcga
     8041 gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct ttgatggcag
     8101 caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt
     8161 cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat
     8221 tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg
     8281 tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaacgg
     8341 tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca
     8401 accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga
     8461 ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg
     8521 taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga
     8581 agccttaatc ccgctgctgc cgccagagta tgcctgcggc gtgatttggg cgcagagtaa
     8641 gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt
     8701 accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt
     8761 gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc
     8821 agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg gcttctgaca
     8881 taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggagct
     8941 aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat
     9001 accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac
     9061 gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc cgtcacaggc
     9121 acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct
     9181 tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca
     9241 tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa
     9301 accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg
     9361 cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc
     9421 atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata cggttctacc
     9481 tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca
     9541 atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg
     9601 cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga
     9661 gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca
     9721 gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg
     9781 tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt
     9841 gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg
     9901 aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac caggccgcca
     9961 gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct ttacccagcc
    10021 agtaccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga cgaaactggc
    10081 gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa agtccggttt
    10141 cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc ggcgtaatac
    10201 gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga atcccttgag
    10261 ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg aattgcgtgc
    10321 ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct ttcctctttt
    10381 ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg gctggaaacc
    10441 cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt cgatgaagtg
    10501 gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt tctgagcggt
    10561 tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg cgcgctggcg
    10621 gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg tacggtcgaa
    10681 gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg gggcagtaaa
    10741 aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc caaaggaaat
    10801 agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc actaactccg
    10861 cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt gctggaaagt
    10921 aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta tacggtgacg
    10981 ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg tgatacggtt
    11041 tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat gggcgatccg
    11101 ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt ggtgccgggt
    11161 aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg cgatttcacc
    11221 caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg tattgatatc
    11281 gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc caccctgaca
    11341 gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc cgggcacgac
    11401 gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag catcaacggg
    11461 atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg ggtcaaaacg
    11521 ttggcactca tgctttatca acttgcctgg cagaaataag gagtcataaa tgggatattt
    11581 aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg ttaaacacgg
    11641 taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg actttgaaaa
    11701 ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg attatctggt
    11761 cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca ttgaaacgtt
    11821 cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat ttgccttaag
    11881 cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta acgcccaggc
    11941 cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag gccatgcacc
    12001 gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag gtatggatga
    12061 tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca tgcatatcct
    12121 ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg aacatggtgc
    12181 ctatattctt tccggtcagg gggtttata
//
LOCUS       DAJVRL010000106        12153 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVRL010000106 DAJVRL010000000
VERSION     DAJVRL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.106"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            127..996
                     /locus_tag="OQN02_003212"
     CDS             127..996
                     /locus_tag="OQN02_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCS1643906.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1199..1552)
                     /locus_tag="OQN02_003213"
     CDS             complement(1199..1552)
                     /locus_tag="OQN02_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1643907.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1690..3336)
                     /gene="groL"
                     /locus_tag="OQN02_003214"
     CDS             complement(1690..3336)
                     /gene="groL"
                     /locus_tag="OQN02_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1643908.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3380..3673)
                     /locus_tag="OQN02_003215"
     CDS             complement(3380..3673)
                     /locus_tag="OQN02_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1643909.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3949..5205
                     /gene="yjeH"
                     /locus_tag="OQN02_003216"
     CDS             3949..5205
                     /gene="yjeH"
                     /locus_tag="OQN02_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1643910.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="OQN02_003217"
     CDS             complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="OQN02_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1643911.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6034..7470
                     /gene="aspA"
                     /locus_tag="OQN02_003218"
     CDS             6034..7470
                     /gene="aspA"
                     /locus_tag="OQN02_003218"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1643912.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7588..8889
                     /gene="dcuA"
                     /locus_tag="OQN02_003219"
     CDS             7588..8889
                     /gene="dcuA"
                     /locus_tag="OQN02_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1643913.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9005..9343
                     /gene="cutA"
                     /locus_tag="OQN02_003220"
     CDS             9005..9343
                     /gene="cutA"
                     /locus_tag="OQN02_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1643914.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9319..11016
                     /gene="dsbD"
                     /locus_tag="OQN02_003221"
     CDS             9319..11016
                     /gene="dsbD"
                     /locus_tag="OQN02_003221"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1643915.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11053..11628
                     /locus_tag="OQN02_003222"
     CDS             11053..11628
                     /locus_tag="OQN02_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1643916.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11735..11810
                     /locus_tag="OQN02_003223"
     tRNA            11735..11810
                     /locus_tag="OQN02_003223"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11768..11770,aa:Phe,seq:gaa)
BASE COUNT         2930 a         3140 c         2981 g         3102 t
ORIGIN      
        1 tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa
       61 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
      121 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
      181 ttcattgcac tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
      241 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
      301 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
      361 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
      421 cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg
      481 ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg
      541 cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc
      601 tcactgttag atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa
      661 tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg
      721 ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg
      781 cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc
      841 ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg
      901 aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta
      961 caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct
     1021 ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta
     1081 aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg
     1141 cctgataagc gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc
     1201 agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca
     1261 acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct
     1321 ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg
     1381 aaagccagtt gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct
     1441 gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac
     1501 ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc
     1561 ccataatcaa tgagctggac gccactcgcg tcatccgtgt ctgaatctta tactagaaag
     1621 atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaagt
     1681 gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc
     1741 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag
     1801 gccagccaca gaagctgcgt actgcagagc agaacgggtt actttggttg ggtccaggat
     1861 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
     1921 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg
     1981 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
     2041 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
     2101 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt
     2161 tttctctttc atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc
     2221 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
     2281 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat
     2341 gatagtggtg gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt
     2401 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
     2461 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac
     2521 tttcacgatg ccacgcatgg tgttaacaac cagagttgct agcgcttcgc cttcaacatc
     2581 ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat
     2641 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
     2701 tgcgccagtt tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat
     2761 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc
     2821 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
     2881 agcggagatg gtaccaacct gagcaatcgc tttagaatca gagcacggta cggacagcgc
     2941 tttcagttct tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg
     3001 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
     3061 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat
     3121 ctgcgcaccc atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
     3181 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggccttttgg
     3241 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
     3301 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
     3361 gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
     3421 tcaacacttc ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa
     3481 cgatgtcgcc aactttcacg tccagcggct tcacttcgcc attttcaagg atacggccat
     3541 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
     3601 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
     3661 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
     3721 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
     3781 agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc
     3841 tcacatttca tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg
     3901 aaaatctacg cagttgccca aaatttgggc gcaatcggat atcaacccat gagtggactc
     3961 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
     4021 ggcgtgtttg ccgttcctgc gttagctgca ctggtagcag gcaataacag cctgtgggcg
     4081 tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
     4141 cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
     4201 gagcgagtca ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta
     4261 caaattgccg ccggattcgg ccaagcaatg tttggctggc atagctggca actgttgttg
     4321 gcagaactcg gtacgctggc gttggtgtgg tatatcggta ctctcggtgc cagttccagt
     4381 gctaatctac aaaccgttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg
     4441 gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc
     4501 gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc
     4561 catcttgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt
     4621 ctgctgctgg caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc
     4681 tatggtgaac aaatggcggc ggcagcatcg cttccaaaaa ttgtagtaca gttgttcggt
     4741 gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac
     4801 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
     4861 tacctggcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc
     4921 tgctgcgtgg tgagcacgtt ggtaattcat gccttagaga tcaatctgga cgctcttatt
     4981 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa
     5041 ttattgcaag gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
     5101 ctggcaatgg tcggctggaa aagtctctat gcgctgatca tgctggtggg gttatggctg
     5161 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
     5221 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata
     5281 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
     5341 agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa
     5401 aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat
     5461 aatcagcgaa acacttttaa tcatctccgc cgcagggttt tcacccgccg ccattttttg
     5521 ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga
     5581 cgtgaatata accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa
     5641 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
     5701 tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgccttc
     5761 agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta
     5821 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
     5881 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat
     5941 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
     6001 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
     6061 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
     6121 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
     6181 ggtatggtaa tggttaaaaa agccgcagcc atggcaaaca aagagctgca aaccattcct
     6241 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
     6301 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
     6361 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
     6421 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
     6481 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
     6541 ctgcgtgaag gctttgaacg taaagctgtc gaattccagg acatcctgaa aatgggtcgt
     6601 acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc
     6661 ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
     6721 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
     6781 gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa
     6841 gcgacctctg actgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg
     6901 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg
     6961 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
     7021 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
     7081 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggaacc ggtcattggc
     7141 caggccatgt tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
     7201 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
     7261 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
     7321 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
     7381 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
     7441 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca
     7501 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
     7561 acttgttaaa aaacaaggaa ggctaatatg ctagttgtag agctcatcat agttttgctg
     7621 gcgatcttct tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg
     7681 gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc
     7741 attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat
     7801 ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca
     7861 ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca
     7921 ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctctct
     7981 actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt
     8041 tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc
     8101 atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac
     8161 tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagagggctt ggttgaactg
     8221 cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg
     8281 ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt
     8341 gaaaaaccgc tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact
     8401 ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa
     8461 gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt
     8521 tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg
     8581 ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca
     8641 aaagcactga tgccgatggc tctggcactg aacgtttcac cgctgaccgc tgttgcttct
     8701 ttcgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta
     8761 cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc
     8821 atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc
     8881 atgctgtaat gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat
     8941 ccttcccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt
     9001 gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc
     9061 ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg
     9121 cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga
     9181 atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg
     9241 cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg
     9301 agacacagat tacctctcat ggctcaacgc atctttacgc tgattctgct actttgcagc
     9361 acttccgttt ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat
     9421 caagcctttg cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc
     9481 aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt
     9541 gccgacgagc agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag
     9601 atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg
     9661 ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa
     9721 accgttccgt taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg
     9781 cagcaagagc agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt
     9841 attggtatcg cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg
     9901 ctgggtggta aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg
     9961 caggggatgg cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag
    10021 ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcgccatcgt ctttaccttg
    10081 ctggcgatgt caatgtttgg cttgtttact ctacaactcc cctcttcgct gcaaacacgt
    10141 ctcacgttga tgagcaatcg ccaacagggc ggctcacctg gcggtgtgtt tgttatgggg
    10201 gcgattgccg gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg
    10261 tatatcgccc aaagcgggaa catgtggctg ggcggcggca cgctttatct ctatgcgttg
    10321 ggcatgggcc tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc
    10381 ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc
    10441 ttcctgctgg agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt
    10501 gtcgcattct ttggctgggc ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc
    10561 gtggtgcaaa taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg
    10621 gcatttggtg cgacgcatac cgcgcaaact caaacgcatc tcaactttac acaaatcaaa
    10681 acggtagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat
    10741 ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg
    10801 caggtgcaaa aagcgttagc agacacggtc ttacttcagg ccaacgtcac ggccaacgac
    10861 gcacaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt
    10921 tttgacggac aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa
    10981 accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg
    11041 gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta
    11101 caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac
    11161 gagctacgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt
    11221 agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa
    11281 caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg
    11341 ggctgtctat ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa
    11401 ctggccgatc agcaaaaaag cgctgcctac gatttcaccc acgaactgtt aaccacgctg
    11461 gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta
    11521 agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa
    11581 gatatcctgc gcttcgcccg ctgccgccag ggtggtgcat tgacctgaca gaaacacaga
    11641 aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac
    11701 gaaatgtgca gattacggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca
    11761 ggggattgaa aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc
    11821 aacggacctc cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa gcgttctgcc
    11881 tccaaatcaa ttctaccgaa ctcaaccaga ttctccccac atcaccagca atttgcgagc
    11941 atatcccaat ttgggaaaat ttgttcctga gctatagcac taactgacgt gaaatgtcgt
    12001 gcggccccgt gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gtatccataa
    12061 agcacaacac cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat
    12121 gtgtcagtcc gcttgctcat gcaaagacta aca
//
LOCUS       DAJVRL010000107        11921 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVRL010000107 DAJVRL010000000
VERSION     DAJVRL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.107"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..2338)
                     /locus_tag="OQN02_003224"
     CDS             complement(<1..2338)
                     /locus_tag="OQN02_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1643917.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNV"
     gene            complement(2272..2895)
                     /locus_tag="OQN02_003225"
     CDS             complement(2272..2895)
                     /locus_tag="OQN02_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCS1643918.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2892..4580)
                     /locus_tag="OQN02_003226"
                     /pseudo
     CDS             complement(2892..4580)
                     /locus_tag="OQN02_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="OQN02_003227"
     CDS             complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="OQN02_003227"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1643919.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7503..8225
                     /locus_tag="OQN02_003228"
     CDS             7503..8225
                     /locus_tag="OQN02_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1643920.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="OQN02_003229"
     CDS             complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="OQN02_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCS1643921.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10297..11892)
                     /locus_tag="OQN02_003230"
     CDS             complement(10297..11892)
                     /locus_tag="OQN02_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCS1643922.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3307 c         2946 g         2911 t
ORIGIN      
        1 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcaa
       61 gctgaggaac ggcaactttg atcccggcgt tctgaaaact gtactgacca ttacgggtat
      121 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactggtacc cgggcttcat
      181 acagggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgaa agcagcgact
      241 gctgttccac gcgatcgcgt tccagcgtca ataacgcttc atcaattggc tccgggaaag
      301 taatcagcat cttcgcggtt tcgcctggct ggtacagcgt tttatccgcc acgatatcta
      361 ccgtaccagt atgcgccgtg ctgcccttac cgctgacggc atgacttaac ccagcgagaa
      421 ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg aaattgacgg
      481 taaaggattt gccgcctgac ggtagctctc cgctatggct cgtgcggtct tcgagacgca
      541 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
      601 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
      661 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
      721 cggttaacaa atagcggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
      781 tggcgtcgga caccgtttcg ctgccttcca gcgacacggg gaaacgtccg gcataacgca
      841 aatcgttacc gaccattgat aattgctgag cgcgcaaact taactgcacg cgggcatttt
      901 ttaccggctc gccatccggg tagagcagtt gcagtttgcc gctgaccgct tcgccagttt
      961 tgaactcttt tttgtcgaga gctaaaccaa tctcgaaatg tggcttgatg tagtttgcca
     1021 cgcgaaaact gctgctatag acctgattgc ggtaagcaag acgtaactca taacctccgg
     1081 caacggcatt ttctggcagg cggaaacttc cctgcccgcc attgcgcgca tccagcgtga
     1141 catcgacggt ttgcaacaga ctgccgttag cgtccagcac cgaaagcttc gccggggcgc
     1201 tgacgatggg ggatgaatgc aacggatcgc ggaactcgcg gcccatcact ttaacatcga
     1261 cacgatcgcc tgcgcgatat agcgggcgat cggtaaaaat atacaagcgg gtgttgtaga
     1321 tttcgctttc gtagaagaag ttctcggaga caaaaacgcc gccttcagca tccttaccca
     1381 gaatgtatga acgttctggc gatatatgtt gtaactgcaa agtaccgctg tcatcggtca
     1441 ccccgcgggt catcacgcca agaccgtcag tccacaagat ctctgagccg ggcttcgctt
     1501 caccctgttt tttacccgcg gtccatacca gaagctcgtt acctgacact ttgctaagcg
     1561 ccacggtatc ggaaacaaac accaccgtcg tcgcccgata cccacctacc atcgcctcga
     1621 cgaggtacag ccccggctct tgttggccga gaggaatata aatattaccc ggctgcggcg
     1681 agatgaaatt gctggatgcg ccttccagtt ttaccccttg ctgcggctcg accggtttag
     1741 cctgccatag tggataacgg aactgttcca ccaagggata ttttttcagc ggggaaaact
     1801 ggttgttctg tacataacgg gaaggtttaa taatggcatt gccgagatgt aattcgggta
     1861 atgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc acgcggcgag
     1921 atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagcccg tcgcccagat
     1981 attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat gccatcgggt
     2041 caggaatacg atataggcga acgtccacgc cgccgtactc ttccatctga tagcgccgat
     2101 aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta aaactgctgt
     2161 cagcaagcaa aaagaatgtt cccccggcgg gcggcgcata gttactggaa ggaagcgaat
     2221 catcagcttt agcaagccct gttcccacaa gagacaggga caagcacgct gctatcgcgc
     2281 cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg gtatccatcg
     2341 ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     2401 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     2461 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cttggccaat
     2521 aaactggctg ttgtactgaa tcaaattaat cgcggtcacg taggggccgt ttttcccgct
     2581 cccctgattc cagttttgcg ccagttgacg ctgttcaggt gttagcgtca tctctggcgg
     2641 caaatactgg ctagataaac cgttactttt tagccattta ctgtcgtgaa ctttcagcgt
     2701 ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     2761 cccctggcgg agctgttctt gtgcaatgcg tacaaaccag gcgcgaaaca gcctggattg
     2821 ttcgacattc agcatttcgc tatgggcaac aacacaacac agccagcaaa tcagcgccag
     2881 cagcccgtgt ctcatagcgg ctgccaggtt atgggtagcc actgccaggc gacaccgggt
     2941 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     3001 attaataaag tttgtgcggc gttataaaaa accggttcga gattcttcgg cagactggtc
     3061 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     3121 ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt ttgcagggcg
     3181 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     3241 acacggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     3301 tgaccgtatc gggaactcac ttcgcgacac cagatttgcg cttcgccctg ctgagtctgg
     3361 cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     3421 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     3481 gtttgcaatt ttgagtcttc ataccagcac agccccgcgg caccgtctaa cgccccattc
     3541 agcttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     3601 ggcaccgcca cacagaagct ggctccggca ggcatactgt tccagaccgg cgtaaaatcg
     3661 aaactggcat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     3721 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta accgctggta gccaaaaccc
     3781 agcaacctgg cgctgaccac gatgcgcagg cgtactggcg ttttttcggc tgtacgctct
     3841 tccaggccaa agctggcttg ccagcgtttt ttaccgctca acaaatcact tgcgatcgcc
     3901 gtggcttcgg tatcctgctg atcatcttta aacaacatat ccgtactgga aaacaccagc
     3961 atcttgtcct gatatgtcgc gaacatcagg gcgttattgc cgttatagcg caactgataa
     4021 acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttgct taactagctg
     4081 tcgctggtag cggcaaacaa caatggctcc agcagtttgc ttaaaccact gcgctggatc
     4141 agcaccatgt aatgtgaaag atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc
     4201 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaatg
     4261 cggcgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     4321 tcttcgctca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     4381 agagagtcgc tgtctagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     4441 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     4501 ccagcggaaa gcagtgctat tgcgccaaaa cctacaagac catagaaccg ccagcctttc
     4561 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     4621 acagccaaac tttaccgcgc cctaatacga caaaagccca gactttgcag cctggacttt
     4681 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
     4741 tggctcgtcg tcaacgtcca cttccggagc aatttcatca tccccttccg cggcactgcc
     4801 gtcgatggta tccagatctt cctcgtcaac cggttcagca acacgctgca gacccactac
     4861 gttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ccacgatgct
     4921 gatttctgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
     4981 gcagtcatct acctgcaccg cgccaaccac taaaccgtta cgttcggtaa ccttgatgga
     5041 gataacccct ttcgtcgcac gcgacttggt tgggtattcc gccactgcag tacgtttacc
     5101 gtaaccgttt tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
     5161 gactttatcg ccttcaccta agcgaatacc gcgcacacca gtggtattgc agcccatcgc
     5221 acggacagaa gactctttaa agcgcaccac tttaccttcg gcggagaaca gcattacttc
     5281 atcttcgcca ctggtcagat caacgccgat cagctcatcg ccgtcaacca gtttgatcgc
     5341 cactttaccg gcggtacgca gacggttgaa ttcggtgagg acggttttct tcacagtacc
     5401 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc
     5461 ggtgatacgt tcgtcctgtt ccagcggtag caggttgacg atcggacgac cgcgcgcgcc
     5521 acgagtggct tccggcaact ggtaaacttt catcgaatag acgcgaccac ggctggagaa
     5581 gcacagaata tggtcgtgcg tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
     5641 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
     5701 ctgatattta acgtagccct ggtgagagag cgtcacgacc acatcttcct gggtgatcag
     5761 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
     5821 ctgttcacga accagctcca gctcttcgcg gatcacttcc atcagacgat cggcgctacc
     5881 aagaatacgc aacagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
     5941 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
     6001 ttcggtcagg tagtacagac catcacgcac gccgaactca ggctccagcc attccggacg
     6061 cgcagcatcg tcgccagcac gttccaacat ggcggcaacg ttgcccagct gccacggatt
     6121 agcaaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
     6181 cgggtcaatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
     6241 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
     6301 cgcgatgatg tctttcaggt tcatgatctt cggctgacca tggtgcaatg ccaccatgtt
     6361 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
     6421 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
     6481 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
     6541 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gtccggtttt
     6601 ggcgtcaact tccacttcag cacgggcgcg gatatatacc ttgccgcgac cggtacggta
     6661 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
     6721 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacaacc
     6781 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
     6841 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
     6901 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
     6961 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
     7021 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
     7081 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
     7141 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
     7201 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
     7261 acggccaaca atgaccgaca tcgcataatc cagataggag ctctttagct cttcctcaat
     7321 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
     7381 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
     7441 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
     7501 caatgaatgc cgaaaaatcg ccggaaaacc ataacgtaga ccacgaagag atcgctaaat
     7561 ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca
     7621 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattt ggcaaaaagg
     7681 tgctcgatgt cggttgtggc ggcggcattc tggccgagag tatggcgcgc gaaggcgcga
     7741 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg
     7801 aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
     7861 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtaccc gatccgcagt
     7921 cagtggtcag agcctgtgcg caactggtga aaccaggcgg cgatgtcttt ttctcgacgc
     7981 ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg cgcggaatat attttgcgca
     8041 tggtgcccaa aggtacgcat gatgtgaaga agtttattaa accggcagag ctgcttggct
     8101 gggtggatca gaccagttta aaagagcgac atataaccgg gctacattac aacccgatca
     8161 ctaattcctt taagctcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
     8221 agtgaggttg atgtttggcc gcgccaatgc ctgatgcgac gcttgcagcg tcttatcagg
     8281 cctacaaatg ctctccgtag gccagataag gcgtttacgc cgcatccggc aattgtgatg
     8341 gtcagacagc gattaccatt tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc
     8401 atcactccct ttctgccacg ccacgctgcc gcgcagggac actcgctgac tgatattgcc
     8461 cgtgactccc acttttattt caccccgtag cttcaccgca tcgtcactga tagtgctgcc
     8521 atcttcttca atttccgtcg catggggatc gtgatagtaa ttcagatcta atgttggtgt
     8581 gacaccaacc gcggtacgcc attcgctgtg taaacccagc cgcgtctgaa tatcatcccc
     8641 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
     8701 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataccc
     8761 gacttccagc gaggcgataa tccccgacga atggtaacga tccacgccat cttcatgttc
     8821 ggaaacatca ttgttaaacc acgcgtattg cagccagcta tccagccagg ccccctgctt
     8881 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     8941 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tgattatcac tgtagccacc
     9001 aaccgcccca agcatccact cgccatccgt gccccagcgc ccgctaaaca agccgccgct
     9061 aagctgcacc gtagaagtgt cttcctgctg agccagttgc cctgccgccg tgtaatgata
     9121 acgtccgccg ataacacgta aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg
     9181 ctccatcata aacgcctgat ttgccgcccg caggttattg agataaccgc caactttggc
     9241 attcagcacc ggctggtaag caggcgtagg ggccgggtcg ggggttgggt caggatccgg
     9301 cgtgggatca ggatcgggag tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg
     9361 cgatcgcaga taccagtcgt tgttatcttc cactagcgtg tagtcatacg ctcccatgtt
     9421 gacgtagcca ctgccagcca gactgaactg tgcattgttt tgaaactgcg tgggatcagc
     9481 tgcgaaatca accactttaa tgcctgtcga tgtcggctca ccaatccctg taatggagtt
     9541 aaccaccaca gttgtgttgc cagcagtatt accgttcatc accaattgat cgcttaccga
     9601 gtcatcgccg tttaattcgc tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt
     9661 taccgtcagc gtatcgccag cgacgccatt ttgcaggcta accgcaccgg acgtattggt
     9721 cagatcacca ttaaccgtca cgtcagcaag ataggtcagg ctgtcagttt caaggttggt
     9781 attaagctcg cctgtcaacg tcacgccatc ggtagcattt accagcgtcc catcgccgtt
     9841 aaatagtgag gcattaaggg cgacggaagt atcctgcccg gtcagacgca aaaccgtacc
     9901 atcgctgatg tcgatagtgc cgctggaagt agcatcaatt gactgaatat cctgatccgc
     9961 gccagtaacg aacgttgccc catcaccaac ccacagtgac gaagcataag gaaagatatc
    10021 cgcaacatcg cctttcaggg tgccttcttc gacacgtacc gccgactgcg ttgtaccgct
    10081 ggcggtcagc tccagcgtac ccgcccccgt tttagtaaat gtgctaccct catcctgatg
    10141 ctgcccgctg ctgtcagcca tcagtgcgcc ccactgcgtg tctacccccg catcaactgc
    10201 cacttcaccg tcggcgcgca tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt
    10261 atcgacattg gcagtaatct ccatcacgct ttcttgttac ttatcggtaa agataacctg
    10321 gcggttatag tgggtatctc caagctgcga gttgtcgctc accatcagcg taccggaggc
    10381 gatttgtgtt gtgcccaaat aactattatt gtttgccaga cttacctgcc cgtcgctcgc
    10441 atcgaccacc acgtagacgc catctttccc gtcgccgccg atatgcaaat tgtcgccacc
    10501 tgcaagatct accacatcct gactgccgat aaccgttccg ctgctgccac tgacttcaag
    10561 gccatcattg tatgatgtgc cgctctgcca agtggagaaa tcagagagat cgagcacacc
    10621 gccgtttaac acgatcgact gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc
    10681 ttccagcgaa agcaccgcac catcatcaac gactatatcg ccggttagcg ccatcgactg
    10741 cgcccctgcc agcacgtagc tgccgttttg cgcaatggtg agctgaccag caccttcgat
    10801 gatgccagca aaactgccct gattaacagt aacgttgcca ccagcatcga tatttaaagt
    10861 gccattctga aagcccgtca atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag
    10921 ctcagcctga ttctgatact gatcaatact ccctatcgtc agaccgtagc agtcttgcgg
    10981 atcgtcctgg caatgcgtat cgccgacatt catcagggag ttgctgcgac ccagggtaac
    11041 ttcaccgttt tcaatctgca tctcaccagt aaagtcattg ttatctgcat taagtaccag
    11101 atcgccggaa cctgttttgg tgattaaccc ggtaccagca atagagtcaa cagctccgtc
    11161 attctctgta ttgccgataa ccagcgtttt tccgtcggca atatcaaagg taacttcact
    11221 taagccgaga tacataaagc cacccgccgc agtggaggga ccatctccat agcctgctgc
    11281 gctattgttc tcatcgacta acacgccgcc gttctggctg tagctgtcat caacagaaat
    11341 atcaataaga tagggagccg tcgcgctatt ggtatatatc gccccgccat aaccttcggc
    11401 tgtgttattt gtaaaggcgg tgttatttat tatcgtataa cctgaaggat gcttgctgtc
    11461 gctattatta tcggtaacat cgattgcccc gccatcgcca tcactgtaac ttgttgatgt
    11521 atatgcctgg ttattgttaa aaacatcatc acttaaatag acgtcattat taatggtata
    11581 aattgcgcca cctttgccat cattagcgat attattgatg gcgttagtga cacgtaaatc
    11641 gacggcacca gtatcattag taccagaaga atagattgcg ccaccatagc cgcctgcgac
    11701 gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag caaatatggc
    11761 cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca ttccagtttc
    11821 attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc caccgctggc
    11881 attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DAJVRL010000108        11739 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVRL010000108 DAJVRL010000000
VERSION     DAJVRL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.108"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..932)
                     /locus_tag="OQN02_003231"
     CDS             complement(<1..932)
                     /locus_tag="OQN02_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCS1643923.1"
                     /translation="MSGIFVGLNSHIMEGFDSVLTSVAADTGGWMAHLFSIAVLSLYI
                     TWRAYQTMAGKLVRPFEEIVWDVSRMLIIMIFVTNAGGYLDLVSDAITGLRDGFSGDA
                     SIWARLDTVWEKAQALGEKLYSYDDSTYVALKGTLAQFLVWAGVMLLVGITAIINLIA
                     ELVMLLMLATAPIFIYCLMWSWFRDMFSNWLRIILGCILTSLFSGIALTVVMDYLDYI
                     LAGATKASDANYLTLAVQVFLGTAGAAAVMVLTYKLAEALAGASAQGAAQALAASGVG
                     SARQSASSAAGAVGDKASQNLNSTGSATASSASTA"
     gene            complement(950..1234)
                     /locus_tag="OQN02_003232"
     CDS             complement(950..1234)
                     /locus_tag="OQN02_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HCS1643924.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            complement(1246..1962)
                     /locus_tag="OQN02_003233"
     CDS             complement(1246..1962)
                     /locus_tag="OQN02_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCS1643925.1"
                     /translation="MKLKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKL
                     QQWQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLL
                     TGSGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKIS
                     DTLSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLA
                     EQRRKAQAEQMRTAPIADLN"
     gene            complement(1981..4731)
                     /locus_tag="OQN02_003234"
     CDS             complement(1981..4731)
                     /locus_tag="OQN02_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HCS1643926.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLL
                     LIPAFIELRRLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDAIDVTEFIK
                     HMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHYLATLDMQL
                     NNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGKPFYQRRLF
                     FTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYE
                     ENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYF
                     RGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDD
                     AISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTLKDLGFIMT
                     LSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNAWGEAIAVV
                     PTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQKYRRPE
                     SFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNF
                     LKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKD
                     ARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRSPLTFY
                     LLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFPATQSPEEM
                     CKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYARQMVIMKS
                     PLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDEMLRR
                     AV"
     gene            complement(4741..5040)
                     /locus_tag="OQN02_003235"
     CDS             complement(4741..5040)
                     /locus_tag="OQN02_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HCS1643927.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            complement(5051..5713)
                     /locus_tag="OQN02_003236"
     CDS             complement(5051..5713)
                     /locus_tag="OQN02_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1643928.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            complement(5716..5931)
                     /locus_tag="OQN02_003237"
     CDS             complement(5716..5931)
                     /locus_tag="OQN02_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643929.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            complement(5924..6232)
                     /locus_tag="OQN02_003238"
     CDS             complement(5924..6232)
                     /locus_tag="OQN02_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643930.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            6546..7460
                     /locus_tag="OQN02_003239"
     CDS             6546..7460
                     /locus_tag="OQN02_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1643931.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
     gene            7464..8447
                     /locus_tag="OQN02_003240"
     CDS             7464..8447
                     /locus_tag="OQN02_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643932.1"
                     /translation="MRNLLEYSGSLISNDSLIVAPYDCQKLSYENILPLNNSLIKNWN
                     IQKFSSDYSEIEHLNVINGMGVTLGDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYV
                     EELYELASSVIDELHYMPFHIQKLPCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVK
                     LDDVNHNELSNKWLGNIDLGAPVFENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREG
                     KTVLGFVDVNHKNYINISKYSPHTKDFIALIKYASNVYTCDSSALHIAAGFGVPTECV
                     FNTIPPELRTKYYSKCKSIYVGNKRLEKIQSSEDFQLVKMVENNYREYLYG"
     gene            8440..8928
                     /locus_tag="OQN02_003241"
     CDS             8440..8928
                     /locus_tag="OQN02_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1643933.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            8925..10643
                     /locus_tag="OQN02_003242"
     CDS             8925..10643
                     /locus_tag="OQN02_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HCS1643934.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            complement(11502..11739)
                     /locus_tag="OQN02_003243"
                     /pseudo
     CDS             complement(11502..11739)
                     /locus_tag="OQN02_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         3041 a         2713 c         3019 g         2966 t
ORIGIN      
        1 gccgtggagg cagacgaggc cgttgcgctg ccggtgctgt taaggttctg tgaggcttta
       61 tcccccaccg caccggctgc ggaggaggcg gattgacggg ctgaacctac tcccgacgcg
      121 gcaagtgcct gagcggcacc ctgtgcggat gcaccggcaa gcgcctcggc cagcttatag
      181 gtcaggacca tcacggcggc ggcacccgcc gtgccgagga agacctgaac ggccagagtc
      241 aggtagttgg catcggatgc ttttgtggcg cccgccagga tgtagtccag gtaatccatt
      301 accaccgtca gggcgatgcc gctgaacagg gatgtaagga tgcagcccag aataattctg
      361 agccagttgc tgaacatatc ccggaaccac gaccacatca ggcagtagat aaagatcggg
      421 gcggtcgcca gcatcagcag catcaccagt tcggctatca ggttaatgat ggccgtgatg
      481 cctaccagta acatgacgcc tgcccagacc agaaactgcg ccagagtgcc ttttagagcg
      541 acataggttg aatcatcata agagtaaagc ttctccccaa gtgcttgagc cttttcccag
      601 acggtatcca gccgcgccca gatcgacgcg tcgccgctga atccgtcccg caggccggtg
      661 atggcatccg aaaccagatc gagatagcct ccggcgttgg tgacgaaaat catgatgatg
      721 agcatccggc tcacgtccca cacgatctcc tcgaaggggc ggactagctt gcctgccatc
      781 gtctggtagg cgcgccacgt gatgtacagc gagagcaccg cgatggagaa caggtgtgcc
      841 atccaccccc ccgtatcggc ggccacgctg gtgagcacgc tgtcaaaccc ctccatgatg
      901 tggctgttta atccgacgaa tattccactc atggtgcgtt ctcctgtcgc taattcaggt
      961 cagcaatagg ctcttgcacg cgagctttat caaggtcttt caggatttgc ttatatcctt
     1021 cggcaacatc aggtttagct ttttcacggt gaagggcgga gcgtgcattc tggcaatcgg
     1081 tgggcacaaa attatgatta aaaatgcact ccctgtattt cgccagcatg tcgtcatggt
     1141 gtgccatata ccagtctacg gaggttttat cctcgcagcc agtaagcgcg gccactgagg
     1201 ccagaacaat aaaggtcagt gtcttcagtt tcatggggtc acctgttagt taaggtctgc
     1261 gattggagct gtgcgcattt gctcggcctg cgcctttcgg cgttgttcgg caagcagctg
     1321 gttgcgcatc tcactttgtt tgaccgacat ttcccattga gtggtcaggg cgttcagctg
     1381 gatactccgc gcggacacta cgttcgccag atcctgcgat tctttactgt ccttcgcatt
     1441 ctcgatgcga ctggcgagat tactaatatc gctcagcgtg tcggaaattt tttcctggat
     1501 gtcagaagtg tcctcgacgg acaccgcctg attgaggagc atctgtttgc agctatcccg
     1561 gtacgcctcg ctggcgttct ccgggcagtt gtcgaacagt ttgtatttgt catacagcct
     1621 gtttagttcc ccagcataag acccgcctga gccggttaac agatcgttta aggaaatccc
     1681 cctgtttttc agatttttaa tgtcagccgt caggtttttt gcctgattca ggaatgcctg
     1741 gacgtcccga ataccggtgg ccgttgccag ctgttgttta taagcgttca tctgcgactg
     1801 ataatgttga accgtctgct gccactgctg gagcttctcc gcccactgct ggatgttgct
     1861 ggcgttggct acttcatcaa aaacgggaat accggcctga gccgggacgt gaaagagaca
     1921 caaggcgacg gtcagcgccg tcagggtggt tttcagtttc atggtgttat ccttttgtga
     1981 ttaaacggca cgtctcagca tttcgtcttt ccagtcttcc ggcttcatac cctcctgcca
     2041 gatctcgtca aaaattttca ggttgtccgc actggcgctg agcagcttcg tgtagcgacc
     2101 tagcccggag aggtcgaggg tgatttgtgc gatatacggg cgcacgtcac cacgccgcag
     2161 cggagatttc atgatcacca tctggcgggc gtaggggtcg agggtggtca cgatgtcgta
     2221 aaccgctttc ggcacccgca gcatgtccac gtagtcgtac tccttcgcct gctggttcgc
     2281 catgaagatg cgggtactac actgctcgat aatggccgcc gagatctcgt gcttgcacat
     2341 ctcctccggc gactgcgtgg ccgggaagaa aatgccgttg agctttcgaa tgaccttcag
     2401 catgtcgagg gcgaattttg aaaactccgg gtcctgaagc catttccaga attcatccat
     2461 gaaaatgacc aggcggcggc catcgaggag gctggtcacg cggtagagca ggtagaaggt
     2521 gagcgggctg cgcacgtctt tgtcgttcag gaactccgtg ccgtcgatgc cgaaattatc
     2581 aatattgtcg atatcaaagg tgtccacgtc attatcaaac acccagccaa actccccgcc
     2641 ctgcgcccac tgctggagac ggatgcgcag cccgttcatc cgtgcgtcct tcgtcggggc
     2701 ttcatgcagc acttccagca tgcgggtgat gccgttgcga cgaagctcag gcgggtagtc
     2761 cagcatgatg gtgtccactg cctcgctgag gcgttcttca tcgcgcgggt cgagcggttt
     2821 gccgtccctt gtgcacatca ttcgcatcag cttcttgagg aagttgcggt tacgtttcgt
     2881 tggagcgagc gcaaacgggt taaggccggt cttctcgcct gaacggaaac ggaagtacga
     2941 gccgccctgg aggcgtatcg acatttccgc cgaacggtct ttatcaaaga ccaccgaggt
     3001 gaagcgcttt ttcgtcgccc ccggcgcaaa cgactcagga cgccggtatt tctgcatcag
     3061 atgctggaac atcatcagca gggtcgattt ccccgtcccg gtactgccga gcagcatggt
     3121 gttacccggc ggcttctcgc tgaactcgtc aacatcggcc tggctgttgt gcagattcac
     3181 gtactgacat cccccggacg gggtcggaac aacggcaatc gcctcccccc aggcattccc
     3241 gtcccgcttg tgcgggtgaa agttgtgaaa cgacaccatt tcggcgtagt tgaggctgct
     3301 caccggcacc agacgcgggc gcaggtgata cacacccggc aactgcgcga gatacgccgc
     3361 cgacagcgac tcgcgggaca gggtcatgat gaaaccgaga tccttcaggg tatccgccag
     3421 ggtgttggtg tcccggataa cctgctcgct ggttggggcg ctgacaataa gcgtctggtg
     3481 atatttcccg aatgaaataa tgccggactg aagcatgtcg cgggcgacct tcaggtcttc
     3541 ctgctgggag atcgcatcat cctcggcaga cttcaggcgt ttctcgatgg tgcggatgca
     3601 gccccgcgct tcatccttac ccatgcaggt ataggactgc gtgaacacgt accggcagcg
     3661 ggcataaaga agcgagtcat acagcccggt gatggtttca gtgctgtaat ccttgatttc
     3721 cagcccgcga aagtagctct ggccgtcggt gtggctgacc tggccaatgt cggtggaagg
     3781 gaaaatatcc gtgccgccca gcacgtcata aaacggcgtg cgggtgacgc ggactttctg
     3841 ccacctgccg gtgatcagaa agttgtaaaa cgccagctgg gaggagaaca cgtgcccgtt
     3901 ctcctcataa gtgccaagcg gccgcgccgc gtagcgtgac agcgccgtgc caatcgcttg
     3961 ccggtgctcc tgcatcacct gcagggcatt gtcgagcagg gttttacgtt cgcccaggga
     4021 cttctgtttc atggtcgccc gctcaatgcc ggtgtgcgga ttgaagcaga cggtgaagaa
     4081 gaggcgacgc tggtagaaag gtttgccctc gcgggaggcg taaaacagac gcgcgatttc
     4141 atctgaaaac ggaatcccgg attcacattc aagcgtgtcg gtaaattcgt cgctcaccag
     4201 atgggtatag aaggtaacgg gtagcccctc aaaggagcgg ataacgttgt tgagctgcat
     4261 gtccagggtg gcgaggtaat gttcatcctc gcattcaaaa atgataccgc cgatctccca
     4321 ggtgctgaca aagtcacctt ttttgttctt caccacctgc tcgtgcaggt gggaagacca
     4381 ggggagatag cgggacagcg ggatactctc gttgagccgc atatgtttta taaactccgt
     4441 aacgtctatg gcgtcgtact ggtttgcgac aagcgccttt gtcccgaaat gccgggtcgc
     4501 cgcgcggttg ccacgcgttt taaacgccag caacttcagg tcaaagaaat gaatgtcctc
     4561 ttttgccagc cgtctcagct cgataaaggc aggtatgaga agcaggagaa gccagtaaaa
     4621 acgtgaccag acggccgcca gggtaaggat gcacagggtg aacagcaggg ggacaagcgg
     4681 gaccccccac agcgccgccg gtcgggtcag cgccttgttg aggatggtca tgctgcctcc
     4741 ttaggcggcg aagtaggcac cgaacccgga cgccccggcg agcaggacac ccgcccagaa
     4801 cacgcgctgg agatcgtgcc aggccttgcc gtcaacaagc atcttgtagg cgataaaggc
     4861 caccgcacag ctgatagtga cgactgacat accagttagg ccgaggaaga cccagttaat
     4921 ggcgtcactg cctttctgaa atgcctggtc agcgctggcc gccattgctg gaacagcaga
     4981 cagggcggaa aatgcggcaa tcaggcgggt tgcagcggtt ttgatagtac gtaaagtcat
     5041 gatctcttcc ttaatcagaa gacacagcga ggccgccgcg cacgacatgt ggcggataag
     5101 cagcgatgct ggtcggggtg gtgtcgggtg ccggaattac gccgcgtatg acgctatccg
     5161 ggtaaacagt gttgtcggac gggacggcag gcgcttcccc ggatgaggga gatgctgtct
     5221 gctgttctgc ccgcgttgag ggcacaacgt agccaatacg ctgtacgtag cttgtgttgc
     5281 cgaacgcgct ttccgggcgc tggccggttt caaagttgcc tgagtaatag cagctcaggg
     5341 cgcgctgtaa tgttcctcct cgttgatagc agtcggtgat gattttcgcg gcaacagtca
     5401 tgttgtcaca ggggttaaac atggattcgg cgcttacgcc gaactgcggg aagttggtgc
     5461 tggttatctg catcagccct actgaataac ggtgcttttt ctgctttatg gcctccacaa
     5521 tttttagcgc ctcgtctttc gacgaaggca gatgagaaat cacgcggctc ctgccgccct
     5581 ttaccgggac aatctcagcg atggcatatg gatttaagcc ggattcggtg cgggtgatgt
     5641 cgtggatggt atcggggtga acggcgggag cacactgcat tgcgagctga aacaacgcgg
     5701 cagtggttaa catagtcagt cctttctgta gagcagtacc ttaccggtgg cacagtccgg
     5761 gttgataaat aactgaatgt cgccctggct gttagcttgg gcaacaccga tttttgtaag
     5821 cgtcattgcc agccgatcac ccttcaccga atacacgttg tgcgtgtact cggtcatggg
     5881 ggtgaatacg gcttcaagca gtgaggccgg agcaggcgtg atgtcaggca ttacatttcc
     5941 tattttccag cagtgaggtg attgcatcga tacgtcgcag cctgtcggca ataagtacgg
     6001 atacatcgga ttttttgagt ccctgcgctt tcacagtcag ggtagtgatt tctgcccgca
     6061 gggctgcccg ttcaattagc aattctcgga cgttatccgg cagctccctg agcgaccagg
     6121 tgccattaat ggcacgcgtg atacggtcac gggaggagcg tagcggcggc ttgccgagag
     6181 ttgaataagg attctgacgc agcgccttac ggcggacggt ttgcatgctc atggctgttt
     6241 ctcctgggta tgagtaagcc acccgcgtgc ggtgcacagt gggagacagg gaaggattaa
     6301 acgggaggct taacgataca gaggagtgag agtgttaccc attacaggcg cggcacgtca
     6361 tctctttacg cagtcacaga aaacacctcc ttatcctgac cggggaacgc cgctgctagg
     6421 ttattaaaga gctaatgatt tgattaatac aatagtgctt ataagttggg tattcaataa
     6481 aattataact tatagtatcg atttaagaat gattccaatt tttaggactt tttcagctgt
     6541 gtaaaatgtt ggagatgttt gaaaaaattg aaaaaaaata ctggcaggat aaaatcaaga
     6601 aaatctccaa attcagagta ataaaaatgc aagatctcga tgatgcaaaa gtgatagagg
     6661 tactggcgaa ggtattgacc aaaaaaggta tttccaaggt aaagcacaat agttacataa
     6721 gtaaggtgct acagatcact acatcagcgg gctataagaa gttgaatggt gcgaccaagt
     6781 gggaggttca gcaactcata aaagtagtcc aatccgttgg catggatatg attgagtttt
     6841 tccgtattta ttatgaggat aataaagaaa ttcaagatgc gatctggaac gatggccgca
     6901 gtgaacactc ttgtaaggta tgcctctatc ctgaaggttc ggaagtaaac acagaatact
     6961 cagcactgaa agtagctgac caatggaaca tttttccgac aaatgattta aaagatgaac
     7021 tgctttatga atcaaaacga agcatagaaa gtattgtcat aaatccaaag gtaattagca
     7081 gcactaagta tcgaattgca ctacttgatg acgatgctaa tatcaccgaa agcttaaaag
     7141 aaatcctttc gaacgattat tatcaagtag atacttatga taacttgaaa aaacttactc
     7201 aagctgtcgc aacaaatccg tatgacgcat atgttttgga ctggatcatc ggtgatgaaa
     7261 caagctttaa gttggttaaa gctataaggt cgtcaaaaaa taaaaatgca atgataatcg
     7321 ttcttacagg tcaactatct ggtataaaag atgatgaaat tgtaaagtca attcatgatt
     7381 atgacattgt gggaccatac gaaaagccga ttaaatcagg catcatcaaa tctaacattg
     7441 aaaaatattt cgccagatag attatgagaa atttacttga atattccggt agcttgataa
     7501 gtaacgatag tttgatcgta gcaccttacg attgtcagaa gctaagctat gaaaatattt
     7561 taccattaaa caatagtctt ataaaaaact ggaatataca aaaattctca agtgattact
     7621 ctgaaataga acacctaaat gtgatcaatg gcatgggagt gacattaggt gattctatcg
     7681 tcgggatttc tgtgctgtca gcaatcaaaa aaaagaatcc caagattaaa attaagctta
     7741 ttcgaccgga gactgcaccg tcatatgttg aggaactgta tgaattagca tcctcggtta
     7801 tagatgagct tcattacatg ccttttcata ttcagaaatt gccttgtact gcattgaata
     7861 ttgatgtggg aaatcagcta tattctccag atttccatat tatggaaatg catgattatt
     7921 ttatccaagg tcttggcgtg aaacttgatg atgttaacca taatgagcta tctaataaat
     7981 ggttaggcaa tattgatctc ggagcgccag tttttgaaaa ctacacactt tttgcaccta
     8041 ttgctagcac aaaaataaga agtataccgt cgaagttcta ttatgacata gttgatatgc
     8101 tttctaggcg cgaaggaaaa acggtactag gtttcgtgga tgtgaatcat aaaaattata
     8161 ttaacatatc aaagtattca ccccatacga aagatttcat agcgctaatt aagtatgcta
     8221 gcaacgttta tacttgtgac tcctctgcat tgcatatagc cgctggattt ggtgtaccta
     8281 ctgaatgcgt gtttaataca ataccgccag aattaagaac aaagtattac agtaagtgta
     8341 agtcgattta tgttgggaat aaaagattag aaaaaattca gagtagtgaa gatttccaac
     8401 tggttaaaat ggtggaaaat aattacaggg agtatcttta tggttaaaat aatatcattc
     8461 ttatccctaa tgcttttgtc aattagtgca tatagcaata ttggggatat agttatagat
     8521 ggttcaatcg ctaatgctaa cagtgataaa ggttatgtga ttactgaaaa gcagtttgat
     8581 tcgttcccac gggataccat cgtaacgaca acaccttgga cgccaaccgg taaaaaagta
     8641 aaattcaagg gcgtcgacct aagttatgtt ttaaaactcg ctggcgctaa tggagcaaag
     8701 ctaaaattcc atgctctgaa tgattatgaa atcattgtag acatgaatga cgttgaaaaa
     8761 tataacattt tattggctac agaaatggat ggaaaaaggc ttcaaataag ggattttgga
     8821 ccttattttt tggtatatcc tttagatgaa cattatattg agcttaacac gccacactat
     8881 cttgcacggt tcatttggca gatagacaaa attacggtgc tcaaatgaaa aaaaatttct
     8941 ttatcatttt ggttgctctg cttttgttga tagcattcac tgtgtctagt gttgttaagt
     9001 acaccagtct tttatcggag aaaaatttat acaccgtcgc tggcacccaa gaaaactacg
     9061 gtttctcagt ggcaaaattc attatacagt tgaatgagct ttacacgcta atcaatagcg
     9121 ataatactat tgacgatgtt aggcttaaat tcgatatcct ttattctcgt ttgaatgtta
     9181 tttatgtaaa gagtgaggcg actgcacctc tatataaaca gcaaggctat gaagaaacaa
     9241 ttgattcgat aaacaaaaag cttgaagaca tagacgggct gctttcgcat aatcaccctg
     9301 attataaaaa aataagtaca atcatagctg atataaaacc gctaactaaa accgttacaa
     9361 atcttgctga tatggctgaa atagcacaaa ggaatgacgc tcttaaagat ttcaggagca
     9421 aacgtcagca gttatggact ctgcttttta ttacaggtgg gttgatttcc ttacttcttt
     9481 ttgtcctatt tatttacata agtaaaatta accgattgtt actatcagaa cgggctgctt
     9541 ttgccagtaa aaatgcattt ttaggtatgg taggccatga actcagaaca tctttacagg
     9601 caatagtgtc tatcattgat gtcgtaacca ataatctgag tggagggatt aaatctggtc
     9661 agatagaacg acttgaaact gctgtttcga aaatggagcg ccagttaaat gatttggctg
     9721 aatttgcaaa aattgacaac ggatctgtag agataaaaaa cacctataat agcttgcaag
     9781 caattgttac taacgctgtg caggattgca ttgctattta tgaaaaaaag gatgtcacag
     9841 ttaaaataaa aaacaataat gatgcagtga tttttacaga tgctctacgc cttaatcaag
     9901 taattgaaaa cttaaccagt aacgctatta agtacactga gcgcggggaa gttaatgtag
     9961 attactttat agaaaaaggt aaggtattaa atattgtcat ttctgatacc ggaaagggaa
    10021 tccctaaaga caaactaaag ttcatattta aaccatttac acgagttgta gattcgaaat
    10081 ccactgtacc tggatttggg atgggacttg ctattgttgc agggataatc agactattaa
    10141 aaggtactat tcatatatct agtgaagtaa atgtaggtac aactgttaca gtgagaatac
    10201 ctatcaagtt gggagatagc agccttgcaa cagctgaggt atctgcttca gctcccggag
    10261 atgtcttcgg cgttcttagc ttgcttgtta ttgatgataa tgaaatggcc tgttcgtctc
    10321 tttccagtct gcttactaat gctggataca ttgtcgaggc tacaacctcg cctgaacgag
    10381 ccctggaaaa gcttctgcgt aaaccttatg acgttgtcct gagtgattta caaatgccag
    10441 gcatgacagg tgatgagctg tatgcaactg ttagtagcag tcagaatcct aatcgatcca
    10501 cgcctttcat attcatcagc gcatacagtg atgcacactc tatcggacag gctcctttgt
    10561 taactaaacc tgtcaggtta aaagatataa accttaaaat acatgaggtt atgtcaaatc
    10621 aaggacgagt caaaagtgag taggcattgt cgtaatattt gcgattatag ctttggtgtt
    10681 ctcgatcgtt gcgaattttg aaaatttgtg gagtcaaatg gcgccgaaag cccggcgtag
    10741 ccggttacct ggctataaac tctgccaccc taccccctgc cctgattagc cttaacccgc
    10801 ttacagcaat gaagaagtaa aagtaatccc ttttaacccc ggtgagcggg ctttacggcg
    10861 tttacgacgt aaagggcgtg atttccgggg ccttagcggc ggcagcttgc tgctgacgct
    10921 tagggggatg ttcttcagtt atttcagcga caccagggaa cttaacacgg tagttgcaaa
    10981 gctatacccg ctaaaaatgt ctcagatgcc tctgtacgcg gtcggagtgg tcccggcaag
    11041 gcgtcagccg cagccggggc gtatctccgc gttagcgggc cgaagggccg cttacgagcg
    11101 tgtacccgac aactgaccgc agctccaccc acagcgatga agaagagtag ttacaacacc
    11161 catcattaaa agcgacacta ctcccggcct tcggccgggg gcggcggcgc ttttcagtgg
    11221 tggggagggg cacccggccg gcaaaccggc gccggtttta tgatgagctt tcttttgaat
    11281 gctgtaacta atttggttct ccggtcgggg cccttcgcga agcgatggga gctctttctg
    11341 atgttgccga accggcgcga ccgtcaggtc gctgcaagag cgggcattct ccggagcctg
    11401 tccacagggt aggcgatgaa gaggttatgt gtttacagga tttattaaag gatctttaaa
    11461 agagatacag aactggtgtt taaaaggaac tacgcgccct gaaagaccga cggcaggctc
    11521 agaagatgct ccagcgtggc catagtacgt tcaccgaaga cgtgtgcaac aaccgtcctc
    11581 cgtatcctgt catacgcgta aaacaaccag cgctggcgtg atttagcgcc gacgtaaccc
    11641 cactgttcgt ccatttccgc gcaaacaatg acgtcactgc ccggttgtat gcgtgagttt
    11701 accgactgcg gcctgagagg cggtgtaagt gaactgtag
//
LOCUS       DAJVRL010000109        11697 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVRL010000109 DAJVRL010000000
VERSION     DAJVRL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.109"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..114
                     /locus_tag="OQN02_003244"
     CDS             <1..114
                     /locus_tag="OQN02_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1643935.1"
                     /translation="GPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            111..512
                     /locus_tag="OQN02_003245"
     CDS             111..512
                     /locus_tag="OQN02_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1643936.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            523..1263
                     /locus_tag="OQN02_003246"
     CDS             523..1263
                     /locus_tag="OQN02_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1643937.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1322..1708
                     /locus_tag="OQN02_003247"
     CDS             1322..1708
                     /locus_tag="OQN02_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1643938.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1717..2046
                     /locus_tag="OQN02_003248"
     CDS             1717..2046
                     /locus_tag="OQN02_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS1643939.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2018..4756
                     /locus_tag="OQN02_003249"
     CDS             2018..4756
                     /locus_tag="OQN02_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1643940.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4756..5085
                     /locus_tag="OQN02_003250"
     CDS             4756..5085
                     /locus_tag="OQN02_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1643941.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5085..5783
                     /locus_tag="OQN02_003251"
     CDS             5085..5783
                     /locus_tag="OQN02_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1643942.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5788..6531
                     /locus_tag="OQN02_003252"
     CDS             5788..6531
                     /locus_tag="OQN02_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1643943.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6468..7070
                     /locus_tag="OQN02_003253"
     CDS             6468..7070
                     /locus_tag="OQN02_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1643944.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7131..10611
                     /locus_tag="OQN02_003254"
                     /pseudo
     CDS             7131..10611
                     /locus_tag="OQN02_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            10679..11278
                     /locus_tag="OQN02_003255"
     CDS             10679..11278
                     /locus_tag="OQN02_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1643945.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11300..>11697
                     /locus_tag="OQN02_003256"
     CDS             11300..>11697
                     /locus_tag="OQN02_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1643946.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAYVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT         2741 a         2760 c         3743 g         2453 t
ORIGIN      
        1 ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc ggaaatgccg
       61 aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa atgaaccgac
      121 acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga gacagcgcca
      181 cgttttttga cgggcttccg gcatttattg atgcgcagga actgcctgcc gtggcggtgt
      241 ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc
      301 tgcatattgc cgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg
      361 agagcaccat tttcccggcc ctgaatgatg taccggcact ttccggactc atcgacaccc
      421 tgaacccact cggttttaac tatcaacgtg ataatgagat ggctacctgg gcgatggcgg
      481 aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca
      541 ctggcaaaga cgaaaggtgc ggggacgacg ttctggatgt ataccggcaa cggcgatgcg
      601 tttgcgaacc ctttgtcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa
      661 cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac
      721 tggaaaacga caacccaggg gcagaaatcc gttggtgata cttcggcgac gctggcctgg
      781 cgtccgggtg acagcgggca gaaaaaactg gttcagttgt tcgactccgg tgaagtctgc
      841 gcgtttcgta tcaaatatcc caacggcact gttgatgttt tccgcggctg gctgagctca
      901 ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca ctgtgaaaat cagcggtgtg
      961 gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg gtgttaccgg actgacggtg
     1021 gcaccggcat ctgtcagtgt caaagcggga gcaaccacca cactgacctt tacagtaaaa
     1081 cctgacgggg ccagtgacaa agcgatcagt gtgcattcgt cagatcctca gactgcttcg
     1141 gtgaccctga gcgggcttgt ggccacggtg aaaggcgtga agcagggcag tgtcagcatc
     1201 gtgggcatga cctctgacgg agagtttgtg gcagtggctg cggtgaccgt cagcgcacca
     1261 taacaggacg atactcatca ttgccccggt tatccggggc tttttgcatc cggagaacat
     1321 gatgtttctg aaacaggata cgtttaatta tgaaaaacag tccgtggtgc tcagtgagct
     1381 gtccgggctg cagagaattg aatatctgac gtttgttcag cagcgaacgg caaagtttga
     1441 tgcacaggag ggagaactgc cggaggctga acgacagatt gcttttctgc gtatgggaat
     1501 ggatatcaat gcctggctgg tttcccgctc actgtggaat gctgagcagt ctcaggatgt
     1561 tgagacgctt tgcgcatcca ttatgacaac atggtcgtat gatgcgctgg gcgcgggggc
     1621 ggagagggtt ctgtcgctga gcggtatggg gaccattgag aatgccgggg atgatgatca
     1681 tgaggcgctg acgccggaaa agtcctgacg cgggaaatgc agtttgtcat gcggcttgcc
     1741 cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg atatgtcggc cactgagctt
     1801 ggtgagtggg gcgattattt ccggatgcag agcttcagtg atgtgtggat ggatgcgcag
     1861 tttgcctcgc tgaaggcatt gatcgtgaga atggtgtccg gcagcagcga tgctgcggtg
     1921 gctgatttca gccttttacc ggaagagaac gggataccgg agcgaacgga cgaagaactg
     1981 atgcatcttg gggaaggtat ttccggaggt gtgcgttatg gaccagatag ccaacctggt
     2041 cattgatttg gggattgatg cggcagagtt taaaaatgaa attccccgta tcaaaaacct
     2101 tctgaatggt gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc gttttatgga
     2161 gcgtcagact caggcggccc ggcagacaat gcaggcggct tcttcggctg caacagcagc
     2221 atcagcccat gcgcagacgg tggagaagaa tgcacgggct catgaacgca tggcccgtga
     2281 ggtggaacaa acccgtctgc gcgtggatgc cctgaatcag aaaatgcgcg aggaacaggc
     2341 gcaggccagg gcggctcagc tgggggtaag cagtgctgca gaagtgtata tccgcaaaat
     2401 ggagcgggca ggaaaagcca cacattcgct ggggctgaaa agtgcggcag cccggcggga
     2461 gctgggggtg ttaatcagtc agatggcgcg cggcaatttt ggtgcgctga ggggatccgg
     2521 gataacgctg gctaaccgtg ccggatggac aggcgcactg atgtcgccga aaggcatgat
     2581 gactggcggc gttattggcg gacttgtcgc ggcggtcctg ggtctgggta aagcctggca
     2641 tgacggccgg aaggagggcg agaaatttaa ccgtcagctg gcgctgacgg gacattatgc
     2701 cggtgtcact gtcgggcagt tgtggaaact cagccgggcc atatccggga atggtatcac
     2761 gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc agtggggcat ttcatggaaa
     2821 cgatattggt atggtggcga aagctgccgc acagatggag cgatcggttg gccagtcggt
     2881 cagcgatacc ataaatcagt ttaagcggct gaaggatgat cctgtaaatg ccgcgaaggc
     2941 gctggacaat gcgctgcatt ttttgaatgc cactcagctt gagcagatac gcgtccttgg
     3001 ggagcagggg cggtccagtg atgcggcccg gatcgccatg tctgcgctgg cagaggaaac
     3061 cggtaaacgc acatctgata ttgataataa tctcaatgcg ctgggtagta cgctgcaaac
     3121 cttgtctgac tggtggaagc agttctggga tgcggccatg aatattggtc gtgaagactc
     3181 gctggatgcg cagattgatg cgttacagga aaaaattcag cgcgcgaaaa aatatccgtg
     3241 gacaaacgcc tccacacagg tggagtacga tcagcagcgt cttaacgatc ttcaggagaa
     3301 aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca gaacggaatt accaggagca
     3361 acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg atgaatgaaa cggaagctgc
     3421 acgacatcag cgggaaattg cgcgtattaa tgccatgcag tacgccgacc aggctgtcag
     3481 ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa gccattaaga aaaatacacg
     3541 ggcaacccgt aatgatgagg ccacccggtt attgctgcag tacagtcagc agcaggcaca
     3601 ggtggaaggg cagattgctg ctgccagaca gtcagcaggc attgccactg aaaggatgac
     3661 tgaagcgcat aaacagcttc tggccctgca gcagcgcatc agcgacctgg acgggaaaaa
     3721 gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat gagctgattc aggcgctgac
     3781 gctgctggat gtgaaacagc aggagctgca gaaacagaca gcgcttaacg acctgagaaa
     3841 aaaaacggtt cagctgacca gccagctggc agacaaagaa cgtgcactgc gtgagcagca
     3901 caatctggat attgccactg caggtatggg ggataagcag cggcagcgct accaggcaca
     3961 gttgcgcatc cggcaggaat accggcaaca gttgcaacag cttgagaatg acagtcgcca
     4021 gaaaggcact tacgggacgg aggactaccg gagggctgag gaggtgctga aggggagcct
     4081 gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa ctggaagtgg cgcagggcga
     4141 ctggaaaaac ggtgccatgc gggcgtttca gaattttacg gcggatgcgg ataatgcggc
     4201 gggaacggca gaacagatgt ttacagtggc attcagcagt gccggtaatg cactggcgac
     4261 attctgtacc accggtaagc tgaattttaa atccttcacc tcttccctgt tgtcagatat
     4321 ggccagaatt atggcacaga tggccatgat gcaggcggta aagggcgtcg gttctttatt
     4381 cggcttcacg actaatgctg atggcggtgt ttaccagtct gctgatttga gtcgctacag
     4441 tggcacggtg gttaaccgtc cgacgttttt tgcttttgca aaaggcgcgg gtgtgatggg
     4501 ggaagcggga cctgaagcca ttctgccact gcgtcgcggt gctgatggta agctgggggt
     4561 tgtggcggat actggtggtt caggcatggt gatgtttgcc ccgcagtaca acattgagat
     4621 caataacgac ggcacgaacg ggcagatagg tccggctgcc ctgaaggtgg tttatgacct
     4681 cgggaaaaaa gcggccgcgg actttatgca acagcaggcc cgtgatggtg gccggttaag
     4741 tggagcatac cggtaatgga gacgtttcac tggaaagtgc gcccggatat gaatgtggta
     4801 tcagagccga aagtggtgac agtgaagctg ggcgatggtt atgaacagcg tcgtgcggcg
     4861 ggactgaata accagttgtc gacttacagc gtgacgatac gtgttcgtaa aggtgaacac
     4921 ccatctttaa aagcctttct ggaacggcac ggtggcgtcc gcgcatttca gtggacgcca
     4981 ccttatgact ggaaactgat cagggtggtt tgtcgtaaat ggtcggcaag cgtgggggcg
     5041 ttatgggtga ctgtaacggc cgattttgaa caggtggtga actgatgcag gatatccggc
     5101 aggaaacact gaatgaatgc acccgtgcgg agcagtcagc ccgggtggag ctctgggaaa
     5161 tcgatctgac agaggtcggt ggtgagcgtt attttttctg taatgagcag aacgaaaaag
     5221 gtgagccggt cacctggcag gggcggcagt atcaggcata ccccattcag gggtcggggt
     5281 ttgaactgaa cggcaggggc tgtgctgccc gtccgacact gacggtctct aacctgcacg
     5341 gcatggtcac cgggatggcg gaagatctgc agagtctggt cggcggaacg gtggtccggc
     5401 gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agcgacgccg
     5461 atccggaaca ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa ctgagcgcgg
     5521 tcagtgcctc ttttgtgctg gccacaccaa cggagacgga tggcgcggtt ttcccggggc
     5581 gtatcatgct ggcgaatacc tgtatgtgga cttaccgttc tgatgagtgt ggttacacgg
     5641 gcagggcagt ggctgacgag ttcgacaaac caacaacgga tatccggaag gacaaatgca
     5701 gcaagtgtat gcgcgggtgt gagttgcgca acaataccgg taatttcggc ggtttccttt
     5761 ccatcaataa actttctcag taaatccatg acacagacag aatcagcgat tctggcgcac
     5821 gcccggcgat gtgcgccagc ggagtcgtgt ggcttcgtgg tgagaacgcc ggagggagac
     5881 aggtatcttc ccagcgagaa tatctccggt gagccggagg aacggttccg gatggctccg
     5941 gaggactggc tgcgggcaca aatgcagggt gagattgtgg cactggtcca cagtcatccc
     6001 ggtggtctgc cctggctgag tgaggctgac cggcggctgc aggtgcagag tgatttgccg
     6061 tggtggctgg tctgccgggg ggcgattcac aagttccgct gtgtgccgca tctcaccggg
     6121 cggcgctttg agcacggggt gacggactgt tacacgctgt tccgggatgc ttatcatctg
     6181 gcggggattg agatgccgga ttttcatcgc ggggatgact ggtggcgtca cggtcagaat
     6241 ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc atcagcacaa
     6301 ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac
     6361 tgcggcgatg gtgagctgct gcaccatatt cctgaacaac tgagtaaacg agagaggtat
     6421 accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggaatg gcacgcatct
     6481 gcctttacgg ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg
     6541 ctgaagccat ccgcgcactg gccacacagc tcccggcgtt tcgtcagaaa ctgagtgacg
     6601 gctggtatca ggtgcgcatt gccgggcgtg atgcaggtga aaccgaattg tctgcccgtc
     6661 ttaatgagcc gctggcaaat ggtgcagtga tccacatagt accgcgtctg gtgggagcta
     6721 aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt tatggcggtt gctatatgga
     6781 tgccgggggt aggaattatg gcgagtaatc tgctgttttc tctcggtgcc agtatgacgc
     6841 ttggcggtgt tgcacagatg ctggcaccga aagccagaac tccccgtaca cagacaacgg
     6901 ataacggcaa acagaacacc tgtttctcct cactggataa catggttgcc cagggcaatg
     6961 ttctgcctgt tctgtacggt gaaatgcgcg tggggtcgcg ggtggtatct caggagatca
     7021 gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt
     7081 ttcatgtgaa accgcctgcg ggcggttttg tcgtttatgg agcgtgagga atgggtaaag
     7141 gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaaatcc acgcagttgc
     7201 tgagtgtgat tgatgccatc agcgaagggc cggttgacgg tccggtggat ggattaaaaa
     7261 gcgtgctgct gaacggtacg ccggtcctgg atagcgaggg gaagacaaac ttttccggtg
     7321 ttacggtggt gttccgcgcc ggcgagcagg agcagacacc gccggagggg tttgaatctt
     7381 ccggctcaga gactgtgctg ggtacggaag tgaaatacga cacgccgatc acccggacca
     7441 tcacgtcggc aaacattgat cgtctgcgcc tgaccttcgg tgtgcaggca ctggtggaaa
     7501 ccacctcaaa gggggaccgg aatccgtcgg aagtccgcct gctggttcag atacagcgta
     7561 acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccact tcgcagtatc
     7621 tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt taatatccgg atgcgcagga
     7681 tgacaccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg tcgtacaccg
     7741 aaatcatcga tgtgaaacag tgctacccga acacggcgct ggtcggcgtg caggtggatt
     7801 cagagcagtt cggtaaccag caggtgagtc gcaattatca tcttcgcggg cgcattctgc
     7861 aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg gacggaacgc
     7921 ttaagccagc atacagcaac aacatggcct ggtgtctgtg ggacatgctc actcatccgc
     7981 gctacggcat ggggaaacgt cttggtgcgg cagatgtgga caaatgggcg ctgtatgtca
     8041 tcggccagta ctgcgaccag tcagtgccgg acggatttgg cggcacggag ccgcgcatca
     8101 cctgtaacgc ttacctgacc acacagcgta aggcgtggga tgttctcagt gatttctgct
     8161 cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg caggaccgac
     8221 cgtcggataa agtgtggacc tataaccgca gtaatgtggt gatgccggat gatggcgcgc
     8281 cgttccgcta cagcttcagc gccctgaagg accgccataa tgtcgttgag gtgaactgga
     8341 ttgatccgga caacggtcat gagacggcga cagagcttgt ggaggacacg caggccattg
     8401 tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc cggggacagg
     8461 cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag accgtggact
     8521 tcagcgtcgg cgcagaaggg cttcgccatg taccgggtga tgtcattgaa atctgcgatg
     8581 atgattatgc cggtatcagc accggtggtc gcgtactggc ggtgaacagc cagacccgga
     8641 cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg taccacgctg ataagcctgg
     8701 ttgacggaaa tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac ggcgtgaagg
     8761 tgaaagtgag ccgggttcct gacggcgttg cagaatacag cgtgtggggg ctgaagctgc
     8821 cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg tgagaacgac gacggcacgt
     8881 atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat aacggggcac
     8941 actttgacgg tgaccggcgc ggcacggtga atggtgtcac gccgccagcg gtgcagcatc
     9001 tgaccgccga agtcaccgca gacagcgggg aatatcaggt gctggcgcga tgggacacgc
     9061 cgaaggtggt gaagggtgtg agcttcctgc ttcgcctgac cgtggcagcg gacgacggca
     9121 gtgagcggct ggtcagcaca gccaggacga cggaaaccac ataccgcttc aggcagctgg
     9181 cgctggggaa ttacagtctg acagtccggg cggtaaatgc ccgggggcag cagggcgatc
     9241 cggcgtcggt atcgttccgg attgcggcac cggcagcgcc tgtcactatt gaactgatac
     9301 cggggtattt tcagataacg gtggtcccga aactggctgt atatgacccg acggtgcagt
     9361 ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa accagcgccc
     9421 gctatcttgg cacggcgctg tactggatag ccgccagtat caatatcagg ccgggccatg
     9481 attattattt ttacgttcgc agtgtgaaca ccgttggcaa atcggcattt gtggaggccg
     9541 tcggtcgggc gagcgatgat gcggaaggtt atctgagttt ttataaaggg ttgatcaata
     9601 aaacgcatct cggcaaggag ttgtggacgc agattgataa cggtcagctt gcgccggacc
     9661 tgactgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcaa accgtcaata
     9721 aaaaactgga aaatcagagt gctgcaatcc agcagataca gaaagttcag gttgatacaa
     9781 ataataacct gaacagcatg tgggctgtga agctgcaaca gatgaaggac ggacgccttt
     9841 atattgcgtg gtatcggtgc cggtattgag aatacgccag caggaatgca gagtcaggtg
     9901 ctgctggcgg cagacaggat tgcgatgatt aatcctgcga atggcaacac aaagccgatg
     9961 tttgttggtc agggcgatca gatattcatg aacgacgtgt tcctgaaacg cctgacggct
    10021 ccgaccatta ccagcggcgg taatcctccg gcattttccc tgacacctgg cggacggctg
    10081 acggcgaaaa atgccgatat cagcggtaac gtgaacgcga actccgggac gctcaacaac
    10141 gtcacgatta ataagaactg tcgggttctg ggaaaattgt ccgcgaacca gattgaaggc
    10201 gatctcgtta aaacagtggg caaacctttc ccacgggact cccgggcacc ggagaggtgg
    10261 ccatcaggga ccattaccgt cagggtttat gacgatcagc cgtttgatcg gcaaattgtt
    10321 attcccgcgg tggcgtttcg cggtgctaaa catgagcgga agaataacaa tatttattcg
    10381 tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt ataaccgaac gaccctggat
    10441 aatacgctga tatatacggg tgttattgat atgcctgccg gtcacggtca catgacgctg
    10501 gagttttctg tatcggcatg gctggtaaat ggctggtatc ccacagcaag tatcagcgat
    10561 ttgctggttg ttgtgatgaa gaaatccaca gcaggtatca gtatcagctg aattttataa
    10621 cccagatacg ggcaccagaa atggtgcctt ttttattgca gaaaagcgag aggtaattat
    10681 gcgtaaactt tatgctgcca ttttgtccgc agccatctgt ctggctgtat ccggtgcgcc
    10741 tgcatgggcg tctgaacatc agtccacgct gagcgcgggg tatcttcatg cctcgacgaa
    10801 cgctcccggc agtgatgatc tgaacgggat taacgtgaaa taccgttatg agtttacgga
    10861 cacgctgggg ctggtgacgt cattcagcta tgccaacgct gaagatgagc aaaaaacgca
    10921 ttacagtgat acccgctggc atgaggattc cgtgcgtaac cgctggttca gcgtgatggc
    10981 ggggctgtct gtgcgcgtga atgaatggtt cagcgcgtat gcgatggcgg gcgtggctta
    11041 cagccgtgtg tcgaccttct ccggggatta tctccgcgta actgacaaca aggggaaaac
    11101 gcacgatgtg ctgaccggga gtgatgacaa ccgccacagc aacacgtctc tggcgtgggg
    11161 ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt gaccttgctt atgaaggttc
    11221 cggcagtggc gactggcgca ctgacggttt catcgtgggt gtcggttata agttctgatt
    11281 agccaggtaa cacagtgtta tgacagcccg ctggttcagg cgggcttttt gtggggtgaa
    11341 tatggcagta aagatttcag gtgtactgaa agatggggcg ggtaaacctg tcgtaaattg
    11401 tgcgattgaa ctgcgggcca gaagaaccag tccgaccgtt gtggcatacg ttgttgccac
    11461 ttgcgtgacg gacaataacg gtgcttatgt gattgaggct gagccggggt attacgaggt
    11521 tgcgcttcac tgtaacggct ggcagccaac ccgtgtcggg gatattgatg tggcaccgac
    11581 tgatgcaccg gggacactga acgcgtttct gaatgcacca aaggatggtg atttacgtcc
    11641 agaggtgatg aagcgctttg aggaaatggt ggcgcaggcg cagcagagtg ccggggc
//
LOCUS       DAJVRL010000110        11670 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVRL010000110 DAJVRL010000000
VERSION     DAJVRL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.110"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            597..794
                     /locus_tag="OQN02_003257"
     CDS             597..794
                     /locus_tag="OQN02_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643947.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OQN02_003258"
     CDS             complement(744..1937)
                     /locus_tag="OQN02_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1643948.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OQN02_003259"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OQN02_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1643949.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OQN02_003260"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OQN02_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1643950.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OQN02_003261"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OQN02_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1643951.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OQN02_003262"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OQN02_003262"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1643952.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OQN02_003263"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OQN02_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1643953.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OQN02_003264"
     CDS             complement(10573..10716)
                     /locus_tag="OQN02_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643954.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11670
                     /locus_tag="OQN02_003265"
     CDS             10966..>11670
                     /locus_tag="OQN02_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1643955.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDS"
BASE COUNT         2962 a         2900 c         2993 g         2815 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg aggtcaccca atgggactca
//
LOCUS       DAJVRL010000111        11620 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVRL010000111 DAJVRL010000000
VERSION     DAJVRL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.111"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(272..347)
                     /locus_tag="OQN02_003266"
     tRNA            complement(272..347)
                     /locus_tag="OQN02_003266"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(312..314),aa:Ile,seq:cat)
     gene            906..3157
                     /locus_tag="OQN02_003267"
                     /pseudo
     CDS             906..3157
                     /locus_tag="OQN02_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3493..4470
                     /gene="csiD"
                     /locus_tag="OQN02_003268"
     CDS             3493..4470
                     /gene="csiD"
                     /locus_tag="OQN02_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1643956.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4490..5758
                     /gene="lhgO"
                     /locus_tag="OQN02_003269"
     CDS             4490..5758
                     /gene="lhgO"
                     /locus_tag="OQN02_003269"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1643957.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5781..7229
                     /gene="gabD"
                     /locus_tag="OQN02_003270"
                     /pseudo
     CDS             5781..7229
                     /gene="gabD"
                     /locus_tag="OQN02_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7237..8523
                     /gene="gabT"
                     /locus_tag="OQN02_003271"
     CDS             7237..8523
                     /gene="gabT"
                     /locus_tag="OQN02_003271"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCS1643958.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8761..10161
                     /gene="gabP"
                     /locus_tag="OQN02_003272"
     CDS             8761..10161
                     /gene="gabP"
                     /locus_tag="OQN02_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1643959.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10182..10844
                     /gene="csiR"
                     /locus_tag="OQN02_003273"
     CDS             10182..10844
                     /gene="csiR"
                     /locus_tag="OQN02_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1643960.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10845..11294)
                     /gene="kbp"
                     /locus_tag="OQN02_003274"
     CDS             complement(10845..11294)
                     /gene="kbp"
                     /locus_tag="OQN02_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1643961.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11378..11536)
                     /locus_tag="OQN02_003275"
     CDS             complement(11378..11536)
                     /locus_tag="OQN02_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1643962.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2988 a         2851 c         2898 g         2883 t
ORIGIN      
        1 tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcaatttgc
       61 ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga tgaataaata
      121 cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca cccaccgcac
      181 ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac cccttgtacc
      241 cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact tgaaccagcg
      301 accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta agtatgcaat
      361 aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat aacagatttt
      421 tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg acaccgcctc
      481 cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc actatctata
      541 gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat aactttatta
      601 cattagctga agagttttcg catcattatg atatctgtta cttttcactc cataaaaata
      661 aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt agtttcgtat
      721 gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac agatttaaac
      781 attatccttt tccatataga tattggataa aaataggaca tctgtttgca attactttca
      841 caacaattaa acatttttat gttttcgtat acatcatatt actctaccat tagaggaacg
      901 ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac cccacattgt
      961 tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta gaatatctaa
     1021 actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta aaagacagag
     1081 aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa aacgattata
     1141 aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca cagagtagca
     1201 ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga gatacaatgg
     1261 ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat ttcactcaca
     1321 ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt aacattcctg
     1381 actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc ggacagcaag
     1441 aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag cgtaaatctc
     1501 ttactgctgt tcagaaatca caatgtgaaa tcatcacccc aaaaaacctg gcgaagcgat
     1561 tcttcatgct tttaatgcca cctaccagca aatcagggaa aatatgtctg agtttgcacg
     1621 ttcccattat gggtatatac aaatccctcc agtgacaact ttccgcgccg acggaccaga
     1681 aactcccgaa gaagaaaaag gttactggtt tcacgcttat caacccgaag atctttgtac
     1741 catccataat ccaatggggg atttgcagga ttttatcgca ttggttaaag atgctaaaaa
     1801 atttggtatc gatatcattc ctgattatac ctttaacttt atgggaatcg gggggagtgg
     1861 taaaaatgac ctggattatc cctctgctga tatacgagcg aagatcagta aagatataga
     1921 aagtggtatc cctggctatt ggcaaggtca ggttttgatt ccattcatta aagatccagt
     1981 aacaaaagaa cgtaaacaaa tccatccaga agatatacat ctcactgcaa aagacttcga
     2041 agcaagtaaa gataacatct ctaaggatga atggaaaaac ctccatgcat taaaagaaaa
     2101 gagtttaaat ggaatgccta aaacaacccc caaaagtgac caggttatta tgttgcaaaa
     2161 tcaatacgtt cgtgaaatgc gaaaatatgg cgtacgaggt ttacgttatg atgcagcaaa
     2221 acactcaaaa catgaacaaa tagaaagatc aataacccca ccgcttaaaa attataatga
     2281 gcgattacac aatactaact tatttaaccc aaaatatcat gaaaaaaccg ttatgaatta
     2341 catggaatat ctggtaactt gtcagttgaa tgaagaacaa atgtcatcgc tgctttatga
     2401 aagagatgat ttaagcgcca ttgatttttc attgctcatg aagacgataa aagccttttc
     2461 attcggggga gatctcctaa cccttgcatc aaaaccgggt tccacaatct caagcatccc
     2521 atcagaaaga cggatattga ttaacattaa ccacgatttt cctaacaatg gcaatctttt
     2581 caatgacttt ctatttaacc atcaacaaga tgaacaatta gcaatggcat atatggccgc
     2641 tctcccgttc agcaggcctt tagtttactg ggatggccag gtattaaaat caacgactga
     2701 aattaaaaat tatgatgggt ccacgcgtgt cggtggtgag gcgtggctta ataaaggttg
     2761 ctctacctat cagcagctct acaatgaatt ccacgcatta tatatagata aagcaggaat
     2821 atggagcgca tttgagggtg tatttgcaac taaaaacgtt ctggccttta gtcgtgggga
     2881 ttctgtgaac attaatcact ctcctcatga tggactagtt ataataaata aaggaaacga
     2941 agaagttgaa ggtacctggc ctaacaaatt gcaacctgga atatataaaa acatggggag
     3001 taatagcgtt aacattatta ttaataatac ccgaaaaatt atcccccctt gtaaagtatt
     3061 tacgcttaga ggcggatctc taaatatcaa tattcctagg cgtagcgctc ttcttttagg
     3121 gaaaacagga gaaccgccga actatctcta tttataattt aatgttatat ctgccccgat
     3181 aaaacggggc agataatatg tttagtttac taacggtcat tttgtagtga agccatttac
     3241 tgttttttat cgaccagata atctgttctc taatgttaac tccccctaac ctgttgcttt
     3301 agttattcat ttcctgtctc actttgcctt aataccctac gttaaatgtt actaatttgt
     3361 tgcttttgat cacaataaga aagcaatatg tcgcttttgt gcgcattttt cagaaatgta
     3421 gatattttta gattatggct acgaaatgag catcgccatg tcaccctaca tctcataaga
     3481 ggatcgcttc tgatgaatgc actgaccgcc gtacaaaata acgctgtcga ttcaggccag
     3541 gactatagcg gattcactct catcccgtcg gcgcaatccc cgcgtctgct ggaactcacc
     3601 ttcaccgaac agacgaccaa acagtttctc gaacaggttg ccgaatggcc cgtgcaggcg
     3661 ctggagtaca aatcgtttct gcgttttcgg gtaggcaaaa ttcttgacga tctgtgtgcg
     3721 aatcagctgc aaccattgct gttgaagacc ctgctaaacc gtgctgaagg tgcgctgttg
     3781 atcaatgcgg taggtatcga tgatgtggcg caggcagatg agatggtgaa gctggcaacg
     3841 gcggtagcgc atctgattgg tcgctcaaat ttcgatgcga tgagcggtca gtattacgcg
     3901 cgtttcgtgg tgaaaaatgt cgataactca gacagctatc tgcgtcagcc gcaccgcgtg
     3961 atggaactgc acaacgacgg cacgtatgtc gaagagatca ccgattacgt gctgatgatg
     4021 aaaatcgatg agcaaaacat gcagggtgga aattcgttgc tgctgcatct cgatgactgg
     4081 gaacatctgg accactattt ccgccatcca ctggcgcgtc gcccgatgcg ctttgccgcg
     4141 ccgccgagca aaaacgtcag caaagatgtt ttccatccag tgttcgacgt tgatcaacag
     4201 ggtcgcccgg tgatgcgcta tatcgaccag ttcgtccagc caaaagattt tgaagaaggc
     4261 gtgtggttga gcgagctttc cgatgccatt gaaaccagca aaggcattct ttctgtaccc
     4321 gttcccgttg gcaaattcct gttgataaac aacctgttct ggctgcacgg tcgcgaccgc
     4381 tttactccgc acccggatct gcgccgtgaa ctgatgcgtc agcgtggcta tttcgcttac
     4441 gccactcacc actaccagac gcatcagtaa gcgcaaagga attgagcgga tgtatgattt
     4501 tgtgattatt ggcggcggca tcatcggcat gtcgaccgcc atgcaactga ttgatgttta
     4561 tccggatgcc cgcattgcgt tgctggaaaa agagtccggc ccggcctgtc accagacggg
     4621 ccacaacagc ggcgtgatcc atgccggggt ctattacacg cccggcagcc tgaaggcgca
     4681 gttttgcctg gcggggaacc gtgccaccaa agccttttgc gatcaaaacg gcattcgcta
     4741 tgacaactgc ggcaagatgc tggtcgccac gtctcagctc gaaatggaac ggatgcgcgc
     4801 cttatgggaa cgcacagcgg cgaacggtat cgagcgcgag tggataaacg ccgtggaact
     4861 gcgcgagcgc gaaccgaata tcaccgggct tggtggcatt tttgtgccgt ccagcggcat
     4921 tgtcagctac cgcgaagtga cggcggcaat ggcaaaaatc ttccaggcca gaggcggcga
     4981 gattatttat aacgccgaag tcagcgccct cagtgagcat aaaaacggcg tggtgatacg
     5041 cacccgtcag ggcggtgaat atgaagcatc gacgctgatt agctgttccg ggctgatggc
     5101 tgaccggctg gtgaaaatgc tcggcctcga accgggcttt attatctgcc cgttccgtgg
     5161 cgagtatttc cgccttgcgc cggagcataa ccagattgtt aaccacctga tttaccccat
     5221 tcccgacccc gcgatgccat ttttgggcgt gcatctcacc cgcatgatcg acggcagcgt
     5281 gaccgtcggg ccaaacgcgg tgctggcttt caaacgcgaa ggctatcgca agcgcgactt
     5341 ctcatttagt gacacgctgg agattttgag ctcgtcgggg attcgccggg tgctgcaaaa
     5401 ccatctacgc tcaggactgg gcgagatgaa aaactcgctg tgcaaaagcg gctatctgcg
     5461 gctggtgcaa aagtattgtc cccggctttc gttaagcgat ctccagccct ggcccgccgg
     5521 tgtgcgggcg caggcagtat cgccggacgg caagctgatt gacgattttc tgtttgtcac
     5581 caccccacgc acgatccaca cctgcaatgc gccctccccg gcagcgacat cagcaattcc
     5641 tattggtgcg catattgtca gcaaggtaca aacgctgttg gcaagccaga gtaaccccgg
     5701 acgcacgctg cgagcggcac gtagtgtgga tgccttacac gccgcattta atcaataacc
     5761 tttgaaaaca ggacgtaaca atgaaactta acgacagtaa cttattccgc cagcaggcgt
     5821 tgattaacgg ggaatggctg gacgccaaca atggtgaagt catcgacgtc accaatccgg
     5881 cgaacggcga caagctgggt agcgtgccga aaatgggcgc agatgaaacc cgcgccgcta
     5941 tcgacgccgc caaccgcgcc ctgcccgcct ggcgtgcgct caccgccaaa gaacgcgcca
     6001 ccattctgcg caactggttc aatttgatga tgcagcatca ggacgattta gcgcgcctga
     6061 tgaccctcga acagggtaaa ccgctggctg aagcgaaagg cgaaatcagc tacgccgcct
     6121 ccttcattga gtggtttgct gaagaaggca aacgtattta tggctacacc attcctggtc
     6181 atcaggccga taagcgcctg attgttatca agcagccgat tggcgtcacc gccgccatca
     6241 cgccgtggaa ctttccggcg gcgatgatta cccgtaaagc cggtccggcg ctggcggcag
     6301 gctgcaccat ggtgctgaag cccgccagtc agacgccgtt ctctgcgctg gcgctggcgg
     6361 aactggcgat tcgcgcgggc attccggctg gcgtgtttaa cgtggtcacc ggttcggcag
     6421 gcgcggtcgg taatgaactg accagcaacc cgctggtacg caaactgtcg tttaccggtt
     6481 agaccgagat tggccgccag ttaatggaac agtgcgcgaa agacatcaaa aaagtgtcgc
     6541 tggagctggg cggcaacgcg ccgtttatcg tctttgacga tgccgatctc gataaagccg
     6601 tggaaggcgc gctggcctcg aaattccgca acgccgggca aacctgcgtc tgcgccaacc
     6661 gtttatacgt gcaggacggc gtgtatgacc gctttgccga aaaattacag caggcggtga
     6721 gcaaactgca catcggcgac gggctggaga aaggcgtcac catcgggccg ctgatcgatg
     6781 aaaaagcggt agcaaaagtg gaagagcata ttgccgatgc gctggagaaa ggtgcgcgtg
     6841 tggtttgcgg cggtaaagcg cacgaacgcg gcggcaactt cttccagcca accattctgg
     6901 tggacgttcc ggccaacgcc aaagtgtcga aagaagagac gttcggcccc ctcgccccac
     6961 tgttccgttt taaagatgaa gccgatgtga ttgcacaagc caatgacacc gagtttggtc
     7021 ttgccgccta tttctacgcc cgtgatttaa gccgcgtctt ccgcgtcggc gaggcgctgg
     7081 agtacggcat cgtcggcatc aataccggca ttatttccaa tgaagtggcc ccattcggcg
     7141 gcatcaaagc ctcgggtctg ggtcgtgaag gttcgaagta tggcatcgaa gattacttag
     7201 aaatcaaata tatgtgcatc ggtctttaac tggagaatgc gaaggaacag caataaagag
     7261 ttaatgcagc gccgcagtca ggcgattccc cgtggcgttg ggcaaattca cccgattttc
     7321 gctgaccgcg cggaaaactg ccgggtgtgg gacgttgaag gccgtgagta tcttgatttc
     7381 gcgggcggga ttgcggtgct caataccggg cacctgcatc cgaaggtggt ggccgcggtg
     7441 gaagcgcagt tgaaaaaact gtcgcacacc tgcttccagg tgctggctta cgagccgtat
     7501 ctggagctgt gcgagattat gaatcagaag gtgccgggcg atttcgccaa gaaaacgctg
     7561 ctggttacga ccggttccga agcggtggaa aacgcggtaa aaatcgcccg cgccgccacc
     7621 aaacgtagcg gcaccatcgc ttttagcggc gagtatcacg ggcgcacgca ttacaccctg
     7681 gcgctgaccg gcaaggtgaa tccgtactct gcgggcatgg ggctgatgcc gggtcatgtt
     7741 tatcgcgcgc tttatccttg cccgctgcac ggcataagcg aggatgacgc tatcgccagc
     7801 atccaccgga tcttcaaaaa tgatgccgcg ccggaagata tcgccgccat catgattgag
     7861 ccggttcagg gcgaaggcgg tttctacgcc gcgtcgccag cctttatgca gcgtttacgc
     7921 gctctgtgtg acgagcacgg gatcatgctg attgccgatg aagtgcagag cggcgcgggg
     7981 cgtaccggca cgctgtttgc gatggagcag atgggcgtgg cgccggatct taccaccttt
     8041 gcgaaatcga tcgcaggcgg tttcccgctg gcgggcgtca ccgggcgcgc ggaagtgatg
     8101 gacgctgttg caccgggcgg tctgggcggc acctatgccg gtaacccgat tgcctgcgtg
     8161 gctgcgctgg aagtgttgaa ggtgttcgag caggaaaatc tgctgcaaaa agccaacgat
     8221 ctggggcaga agctaaaaga cggattgttg gcgatagccg aaaaacaccc ggagatcggc
     8281 gacgtacgcg ggctgggatc gatgattgcc atcgagctgt ttgaagatgg cgatcacagc
     8341 aaaccggacg caaaactcac cgccgagatc gtggcacgcg cccgcgataa gggcctgatt
     8401 cttctctcct gcggcccgta ttacaacgtg ctgcgcatcc ttgtaccact caccattgaa
     8461 gacgctcaga tccgtcaggg tctggagatt atcggtcagt gttttgatga ggcgaagcag
     8521 tagcaccgcc cccatgcagg aggcgacgct gcgcgtcttg tccggcctac gagaatccaa
     8581 gtcggataag gcgtttacgc cgcatccgac aatctgtacg tgaacaggaa taaatctatg
     8641 tcggccggat aaggcggagc cgctctccgg caaaaagaat caataacaat taaacgcgtg
     8701 acccgacgcg ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag aggattcagg
     8761 atggggcaat catcgcaacc acacgagtta ggcggcgggc tgaaatcacg ccacgtcacc
     8821 atgttgtcta ttgccggtgt tatcggcgca agtctgtttg tcggttccag cgtcgccatc
     8881 gccgaagcgg acccggcggt attactggcc tatctgttcg ccggattact ggtggttatg
     8941 attatgcgga tgctggcgga aatggcagtt gctacgcccg ataccggttc gttttccacc
     9001 tatgccgata aagccattgg ccgctgggcg ggctatacca tcggctggtt gtactggtgg
     9061 ttttgggtac tggttatccc actggaagcc aacatcgccg ccatgatcct gcactcgtgg
     9121 gttccaggca ttcccatctg gttattttcc ctcgtcatta ccctcgcctt aactggcagt
     9181 aatttattaa gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct gtgcaaagtc
     9241 atcgctatcc tggcctttat tttccttggt gcagtcgcaa ttagcggttt ttacccttat
     9301 gccgaagtga gcgggatctc aagattgtgg gatagcggcg gctttatgcc caacggtttc
     9361 ggtgcggtat taagcgcgat gttgatcacc atgttctcgt ttatgggcgc agaaattgtc
     9421 accattgccg ccgcggaatc cgacatgccg gaaaaacata ttgtccgcgc caccaactcg
     9481 gttatctggc gtatttctat cttctatttg tgctctattt ttgtcgtcgt ggcattaata
     9541 ccgtggaata tgccggggct gaaagccgtt ggatcttatc gctcggttct ggaattgctc
     9601 aatattcccc atgcgaaatt aatcatggac tgtgtgatat tactttccgt aaccagttgc
     9661 ctgaactcgg ccctgtatac cgcctcaagg atgctctact ccttaagccg tcgcggtgat
     9721 gctcccgcgg taatgggtaa aatcaaccgc agtaaaaccc cgtacgtggc ggtgttactc
     9781 tccaccggcg cagcattctt aacggtggtg gtgaactatt acgcgcctgc gaaggtattt
     9841 aaatttctga tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt
     9901 tcacagttgc ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgaatg
     9961 tggcttgatc cctggctcac ctggctggtc atcagcttta ttacctttgt gttggtagtg
    10021 atgctattcc gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg
    10081 attatctgta ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca
    10141 cccgttcata atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg
    10201 atggctatcg ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat
    10261 tacggatgag tttactgaca tcgcgttatg cacttggcgt tgggccgttg caggaagcac
    10321 tttctcaact ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg
    10381 cgtctatgtc agagcaggag ctgctcgata ttttcgacgc ccgcgccaat atggaagcga
    10441 tgctggtcag tctggcgatt gcccgcggtg gcgatgaatg ggaggcagac gttctcgcaa
    10501 aagcgcatct gctcagtaag ctggaagcct gtgacgccag cgagaaaatg cttgatgagt
    10561 gggatctgcg tcatcaggcg tttcatacag caattgtggc gggctgtggt tctcactatt
    10621 tgctgcaaat gcgtgaacgg ttgtttgatc tggcggcgcg ttatcgattt atctggctgc
    10681 ggcgaacggt gctttcggtg gaaatgctgg aggataaaca cgatcagcac cagaccctga
    10741 ctgcggcggt actgccgcga gatacagcgc gcgccagtga gttaatgcgc cagcatttac
    10801 tgacgccaat tcccattatc cagcaggcga tggctggcaa ttaactactc ttccggaata
    10861 cgcaacactt gccccggata aattttatcc gggcttttta gcatcggttt attcgcttcg
    10921 aagattttat tgtacagatt agcgttaccg tagacctgtt tggaaatggc actcagagtg
    10981 tcgccagact taacggtata aaactggctg gcagtggctg gtgtcgccgt tttcacctga
    11041 tcatcaacac tggcaatacc ggaaatattc cccaccgcaa caaggatttt ctccttcgcc
    11101 tcctgactca ggccgtcacc agtgaccgtc gctttgccgt cggcaatttg aatgttcact
    11161 ttatcggcat caggtatacc ggttttgctc agatgctcct gcaccttctt cgcctgatcg
    11221 tctttatcgt gctgacctgt aaccgcatcc cagagttttt ctccggcatc tttaacaaaa
    11281 ttgaacagac ccatagctac ctcattcgtt aacggaaaca ctcagaaagt gtagcagagg
    11341 aacgcggcgc gttaacgccg cgggggtgag cacaggttta atcgcgggtt tgcacccaga
    11401 acgcgtgaat caaaccgggg atatacccaa gcagtgtcag cagaatatta ataatgaacg
    11461 cccaaccgaa ccctttaccg agcagcacgc cgatcggcgg cagaatgatg gtgatgacga
    11521 ttctccagaa acccatatgt actccctata agaaaattac tcattgttta aaaagagatt
    11581 ttatctctta agcgtagtaa ttttagcgga gcctgccagt
//
LOCUS       DAJVRL010000112        11356 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVRL010000112 DAJVRL010000000
VERSION     DAJVRL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.112"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            404..2044
                     /gene="trg"
                     /locus_tag="OQN02_003276"
     CDS             404..2044
                     /gene="trg"
                     /locus_tag="OQN02_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCS1643963.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2082..3005)
                     /locus_tag="OQN02_003277"
     CDS             complement(2082..3005)
                     /locus_tag="OQN02_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1643964.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3222..4565
                     /locus_tag="OQN02_003278"
     CDS             3222..4565
                     /locus_tag="OQN02_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1643965.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4790..6445
                     /gene="opgD"
                     /locus_tag="OQN02_003279"
                     /gene_synonym="mdoD"
     CDS             4790..6445
                     /gene="opgD"
                     /locus_tag="OQN02_003279"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1643966.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6585..6809
                     /locus_tag="OQN02_003280"
     CDS             6585..6809
                     /locus_tag="OQN02_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1643967.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6872..7408
                     /gene="rimL"
                     /locus_tag="OQN02_003281"
     CDS             6872..7408
                     /gene="rimL"
                     /locus_tag="OQN02_003281"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1643968.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7403..8383)
                     /locus_tag="OQN02_003282"
     CDS             complement(7403..8383)
                     /locus_tag="OQN02_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643969.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8507..9499
                     /gene="tehA"
                     /locus_tag="OQN02_003283"
     CDS             8507..9499
                     /gene="tehA"
                     /locus_tag="OQN02_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1643970.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9496..10089
                     /gene="tehB"
                     /locus_tag="OQN02_003284"
     CDS             9496..10089
                     /gene="tehB"
                     /locus_tag="OQN02_003284"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1643971.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10392..11060
                     /locus_tag="OQN02_003285"
     CDS             10392..11060
                     /locus_tag="OQN02_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCS1643972.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11078..>11356)
                     /gene="tnpA"
                     /locus_tag="OQN02_003286"
     CDS             complement(11078..>11356)
                     /gene="tnpA"
                     /locus_tag="OQN02_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1643973.1"
                     /translation="PPKLAISSLVNSLKGVSGRLLRRDRPDIAVRYYYKGVLWSPGYF
                     ASSCGGAPISVIRQYIEQQQTPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         2925 a         2651 c         2857 g         2923 t
ORIGIN      
        1 tttcaaaagg gtattttggc tttgacatat taggggctat tccatttcat cgtccaacaa
       61 aatgggtgca gtacagttga tagaaatatc ggtcggggcc ttcgtctttc tgattcccgg
      121 ttagcctgaa aacagaaagt ctcaggcacc cgcaggcatc ctatgaggtt tccttaggga
      181 cgaaaataat cacttcacga aattgcgtgc tgttttccag aatttttcgt cattcgggtt
      241 agccagttta gccattcgtt actctcttca ttccaatagc attaattttc tctgcaataa
      301 ttgttgtaaa aatgtgacgc aaagaggttt ttggtcataa gtaattaccg tcaagtgccg
      361 atgactttct atcaggagta aacctggacg agagacaacg gtaatgaata caactccctc
      421 acagcgatta ggttttttgc atcacatcag gctggttccg ttatttgcct gcattcttgg
      481 cggtatctta gttctattcg cattaagttc tgctctggct ggctatttcc tctggcaggc
      541 cgatcgcgat cagcgtgatg ttactgcgga gattgagatt cggaccgggt tagcgaacag
      601 ttcagatttt ttgcgttcag cccggatcaa tatgattcag gccggggctg cgagtcgtat
      661 tgcggaaatg gaagcaatga agcgaaatat tgcgcaagcc gaatcggaga ttaaacagtc
      721 gcagcaaggt tatcgtgctt atcagaatcg accggtgaaa acacctgctg atgaagcctt
      781 cgacactgaa ttaaatcaac gctttcaggc ttatatcacg ggtatgcaac ctatgttgaa
      841 atatgccaaa aatggcatgt ttgaagcgat tatcaatcat gaaagtgagc agatccgacc
      901 gctggataat gcttataccg atattttgaa caaagccgtt aagatacgta gcaccagagc
      961 caaccaactg gcggaactgg cccatcagcg cacccgcctg ggtgggatgt tcatgattgg
     1021 cgcgtttgtg cttgccctgg tcatgacgct gataacattt atggtgctac gtcggatcgt
     1081 cattcgtcca ctgcaacatg ccgcacaacg gattgaaaaa atcgccagtg gcgatctgac
     1141 gatgaatgat gaaccggcgg gtcgtaatga aatcggtcgc ttaagtcgtc atttacagca
     1201 aatgcagcat tcactgggga tgacagtagg gactgttcga cagggcgcgg aagagattta
     1261 tcgtggcacc agcgaaattt cagctggcaa tgcggacctg tcatctcgca ccgaagaaca
     1321 agcggcggct atcgaacaaa ctgccgccag catggagcaa ctcactgcga cggtgaaaca
     1381 gaatgcggat aacgcgcatc atgccagcaa actggcgcaa gaggcttcta ttaaagccag
     1441 cgatggcggg cagacggttt ccggtgtagt aaaaacgatg ggcgctatct ccacgagttc
     1501 gaagaaaatt tctgagatca ccgccgtcat caacagtatt gctttccaga cgaatattct
     1561 ggcactgaat gctgccgttg aagccgcgcg agcgggtgag caagggcgtg gatttgccgt
     1621 tgtcgccagc gaagtacgga cactcgcaag ccgcagcgct caggcagcga aagagattga
     1681 aggcttgatc agtgaatcag tcaggttaat tgacctgggg tcggatgagg tggcaacggc
     1741 agggaaaacc atgagcacta ttgttgatgc cgtcgcgagt gtcacacata tcatgcagga
     1801 aatcgccgcc gcctcggatg aacaaagtag aggcataact caggttagcc aggcgatttc
     1861 tgaaatggat aaggtgacgc aacagaatgc ttctctggta gaagaggcct cagcagcggc
     1921 ggtgtccctt gaagaacagg ctgcacgatt aactgaggcg gtgaacgtat tccgtctgaa
     1981 caaacattct gtgtcggcag aacctcgcgg agcgggtgaa ccagttagtt tcgctacggt
     2041 gtgaaaatgt tcaaggaggg atcgacagat cccttcacct ttcagaacgg cattgatttt
     2101 cgaatagcgt taatcatcaa ctggcaacca gaagagaacg tcgcatctac gcgggtcagt
     2161 attccaatcg gttcgcctgc accatgtccc ggaacaggca gggccaccag cgtggcatga
     2221 cgcaggtcgt cttttacagc gccagaaggg acaaaccaca cgtaatcgta ttcaacggta
     2281 agttgacgag atagcgaagt agacagcgtt tcgatacaac ccgaaggaat tttacagccc
     2341 tggctctgca ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg tgatacaacg
     2401 accggccatt ccagcacccg gcttagcgtt acgttctcct gaagtagcgg gtgattaggg
     2461 cggacaacca gcttcaacga ttcaagaaac agcagttcgt aattaagccc ggtcatcagt
     2521 tcaggatctg acatccgacc aatgccgata tcgatttccc cggtttttaa acccgccaga
     2581 atcatagggt tactcattgt cgcaacttgc aaggtcgtct ctttttgttg ctgatgaaac
     2641 tgacctataa ccgaaggtaa tatccccagt gccgcagtag gtagtgcacc aaccctgacg
     2701 acatcattat taagaccttc tttacgatga agcgactgtc cggcagtgtt gatggcgtca
     2761 agaactctga ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg cgccccctga
     2821 cgaccacgct caaacaagcg agcgcccgtc agctgctcca gttcattcaa tgtattagag
     2881 agcgcaggtt gactcaaatt aagggtttca gctgcgcgcc ccaaagttcc ttgttgtgcg
     2941 acagctacga atgtatgaag gtggcgcaaa cggatgcgct gactaaacag actatttttt
     3001 tccataagcg atgttaaaaa cgaagcggtg tcgctgacaa gtgaagttgt ttgattatga
     3061 taacttgatt gcaaaatatt attaataatt aaagcaatta tgttacagca aaatggataa
     3121 tattgatgtt ttcgcggcga gatcacagtt tgtaaattct tcccgccaga gtgaatgcgg
     3181 ttacctacac tccagattac tgaccactgg aggcagacac tatggcgaac agcatcacgg
     3241 cggatgagat tcgggaacag ttttcgcagg caatgtcagc catgtaccag caagaagttc
     3301 cgcaatatgg cacgctgctg gaactggtag ctgatgtgaa tctggctgtg ctggaaaaca
     3361 atcctcaact gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg aatgttgaac
     3421 gtcatggggc gattcgcgtt gggactgcac aagagcttgc tactcttcgg cggatgtttg
     3481 ccattatggg gatgtacccg gtgagctatt acgatctctc gcaggcaggg gtgccggtac
     3541 attcgacagc atttcggccc attgatgatg cttctctggc gcgtaatccc ttccgcgttt
     3601 ttacatcctt actccgcctt gagcttatcg agaacgaaat tttgcgccag aaagcggcgg
     3661 agattctgcg tcagcgcgat atcttcaccc cacgttgtcg acaactgtta gaggaatatg
     3721 agcagcaggg cggttttaac gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa
     3781 cgtttcgctg gcaccagtta gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg
     3841 aacatcggtt gattgctgat gtggtctgtt ttcctggatg ccatatcaac cacctgacgc
     3901 cacgtacgct ggatattgac cgggtgcagt cgatgacgcc tgaatgcgga attgaaccca
     3961 aaattctcat cgaggggccg ccgcgccgcg aggtaccgat tttactacgc cagaccagct
     4021 ttaaagcact ggaagagacg gtgttgtttg cggggcagaa acagggcacg cataccgcgc
     4081 gctttggtga aattgagcag cgtggcgtgg cattaacgcc gaaagggcga caactgtatg
     4141 atgatcttct gcgtaacgct ggaaccgggc aggataatct cactcaccaa atgcatttac
     4201 aggaaacctt ccgcactttt cctgacagtg agtttttaat gcgtcagcaa gggttggcat
     4261 ggttccggta ccgtctgacg ccttcgggtg aggcgcatcg tcaggcgatt catcctggag
     4321 acgatccaca gcccttaatt gaacgtggtt gggtagtggc gcaacccatc acctatgaag
     4381 atttcttgcc cgttagcgcg gcggggatct tccagtcaaa tctgggtaat gaaacgcaga
     4441 cacgcagtca cggtaatgcc agtcgcgaag catttgagca ggcgttgggt tgtccggttt
     4501 tggatgagtt ccagctttac caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc
     4561 tttaaattct gaccatccgc ctttgcaaaa atttgcctga ttttacaaac gaatcaggct
     4621 catcccatcg acataaaaaa aatgccgatt tatgcatatt ctctcagttc aacaattgga
     4681 ttattaataa atattgtcta gagtgagcgg tcataaataa gcactttctt gccgctgaaa
     4741 acgaccagcg cgggaccatt cacaacacca gaaggactca ctttcaggta tggatcgtag
     4801 acgatttatt aaaggtttaa tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc
     4861 tcttttttct caggcagcat tcgcggcaga ttctgatatt gccgacgggc aaacccagcg
     4921 ttttgacttc tccattctac agtcaatggc gcacgactta gcgcaaacag cgtggcgtgg
     4981 tgcgcctcgt ccgttacctg acacgctggc gacaatgacg ccgcaggctt ataacagtat
     5041 tcaatacgac gccgaaaaat cgctctggca taacgttgag aaccgtcaac tggacgctca
     5101 gttcttccat atgggaatgg gattccgtcg ccgcgttcgt atgttttctg tagatccagc
     5161 aacacatctg gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca acgatgcagg
     5221 tgttgatacc aaacaattag aagggcaaag cgatctcggc tttgccggtt ttcgcgtgtt
     5281 taaagccccc gaactggcgc gccgtgatgt agtatcattt ctcggcgcga gttatttccg
     5341 cgccgttgat gatacatatc aatacggttt gtcggcccgc ggcctggcga tcgacactta
     5401 caccgacagt aaagaagagt tccccgactt taccgccttc tggtttgata cggtaaaacc
     5461 gggggcaact acctttaccg tttatgcgtt gctcgatagc gccagcatta ctggtgccta
     5521 taagttcact atccattgtg agaaaagtca ggtgattatg gatgtggaaa atcacctgta
     5581 tgcgcgcaaa gacattaaac agctgggcat ttcgccgatg accagtatgt tcagctgcgg
     5641 tactaatgaa cgtcggatgt gcgatacaat tcatccgcaa attcatgact ctgatcgtct
     5701 gtccatgtgg cggggcaacg gcgagtggat ttgccgtccg ctgaataatc cgcaaaaatt
     5761 gcagttcaat gcttacaccg acaacaaccc gaaagggttt ggtttattgc aactggatcg
     5821 tgacttctcc cattatcagg acattatggg ctggtataac aaacgcccaa gtctgtgggt
     5881 ggaaccgcgt aacaagtggg gtaagggcac catcggcctg atggaaatcc caacaacggg
     5941 cgaaacgctg gataacattg tctgcttctg gcagccagaa aaagctgtaa aagcaggtga
     6001 tgagtttgca ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt
     6061 agcgcgcgtt atggcgacgc gtaccggcat gggcggtttc ccggaaggtt gggcgccagg
     6121 tgaacactat cccgaaaaat gggcgcgtcg ttttgccgtc gatttcgttg gtggtgatct
     6181 gaaagctgcc gcgccaaaag gcattgagcc ggtgattacg ctttccagtg gggaagcgaa
     6241 gcaaatcgaa attctctata ttgaacccat cgatggttat cgtattcagt ttgactggta
     6301 tccgacttcg gactccactg atccggtcga tatgcggatg tatctacgtt gtcaggggga
     6361 cgctatcagt gaaacatggc tgtatcagta tttcccgcca gcgccggata aacgtcagta
     6421 tgttgacgac cgcgtgatga gttaatcgtt ttttcttcgg caccttcttc gggaggtgcc
     6481 gtctggttaa acacgatccc gctcgcattt ttccctaagt taaatgagta atctgatggt
     6541 gtgtatttca gatacaccgt gtcagccact aacagggagt gcgtatgttt ccagaatacc
     6601 gagatttaat atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac
     6661 acaataaact tgatcatgaa attgccagaa aggaaggttc cgacggtcga gggtacaatg
     6721 cggaagtggt ccgcatgaaa aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc
     6781 tgcagcagga gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt
     6841 ttaacaacag catggattga ctggagataa gatgactgaa acgataaaag taagcgaatc
     6901 acttgaatta catgctgttg cggaaagtca cgtcacacct ctttatcagt taatctgtaa
     6961 aaataaaacc tggttacagc agtcgctaaa ctggccgcag ttcgttcaga gtgaagagga
     7021 cacgcgaaaa acggtgcagg gtaatgtgat gttgcatcaa cgcggctatg ccaaaatgtt
     7081 catgattttc aaagaagatg aacttatcgg cgttatctcg tttaatcgta ttgaaccact
     7141 gaataaaact gctgaaatcg gctactggct ggacgaatct catcaggggc aggggatcat
     7201 ttctcaggcg ctgcaggcat tgattcatca ttacgctcag tctggtgaac ttagacgctt
     7261 cgtgatcaaa tgtcgggtgg acaatccgca aagcaaccag gtcgctttgc gcaatggttt
     7321 tatccttgaa ggttgcctga aacaggctga gttcctgaat gatgcctatg atgatgtgaa
     7381 cttatacgcg cgtattatcg attcataata accctgccag cggcgttcgc gtaatgcgct
     7441 cctcgccgtt aatgactttt ggaccacgga catgaaccgt gtcgccatca aaggcttcaa
     7501 cgacggcgtg gtcggttaac tccacatggt tttcaattat caccgcacct gtaatacggc
     7561 tctcaccttg tattacaacg tgctcgtcga gcagaatcgg tccaccacgt accacggcat
     7621 ttccgccaat caagacatga tgttttaaca cacaattacc ttccacaatg gcatattccg
     7681 ccacctgcga actgtaatga atcgtcggaa tggcatcttc ttctatgcca gctttcacct
     7741 gcgcgtgacc gtagacttta gcgcaatcgc atagccagac gttgttctct tcattacctt
     7801 caacactggc aaaatcaaaa acttcggcgc gatgttcaat aaaagcatac cggacgacgg
     7861 catcgccata aatttgtgcc tggtggacaa tacgcgaggc gctaaccctt gcgcgatcat
     7921 aaatttggag caggagttga tggtcgggcg ttaagccttg tgcggcgacg atcatagaat
     7981 gttgatcaat taaggcgtga ccaaatattc tacattgcac gtaaactaat gagtcatgaa
     8041 tggtgacact gtcacttata tatgcaccct ggctgatttc actattatcg atccagacgt
     8101 tatccgttgc gtaaacctct ccccacagca cgctggtgcc ggtgatacgc gtattaccgg
     8161 aaatcaccgt tccgccgaac gcaatagcat tctggtcgta aatccagcaa tttccttcct
     8221 gtgctaacac cgtttcgcga tcgatccagc cgccagcggt tcctgctatc acatcgttaa
     8281 aatcgctaat ggcgataatc tgccgtaata acacactttt tttagtgcca tcttcctgat
     8341 aactaaaggc tcgctgttct tcacttaggc gatatttgcg catagcgttt tcccacaggt
     8401 gacttactat aaccgtagca aattctgcgg ctctggctat gctcgagaaa attccataaa
     8461 atgcatttca aatatacttt ataaattaaa caaaatgagt aagaagatgc agagcgataa
     8521 agtgctcaat ttgccggcag gctactttgg tattgtgttg gggacgatag ggatgggatt
     8581 tgcctggcgc tatgccagcc aggtttggca ggtcagccac tggttagggg atgggctggt
     8641 gattctggcg atgatcatct ggggattatt gactagcgca tttattaccc gactcatacg
     8701 ctttccgcat agcgtgctgg cggaagttcg ccatccagtg ctgagcagtt ttgtgagttt
     8761 gtttccggca acgacgatgc tggtggcgat tggttttgtt ccgtggtttc gcccactggc
     8821 ggtgtgcatg ttcagttttg gtgtcgtggt tcagttggct tatgccgcct ggcaaactgc
     8881 gggattatgg cgcggatctc accctgaaga agctaccacg cctggactgt atctgccgac
     8941 agttgccaac aactttatca gcgcaatggc ctgtggtgcg ttgggctaca ccgacgccgg
     9001 tctggtgttt ttaggcgcag gcgttttctc atggctaagc cttgaaccgg tgatcttgca
     9061 gcgtctgcgc agttcgggag aattacccac ggcactgcgg acatcactcg gcattcagct
     9121 cgctcctgcg ctggtggcct gtagtgcctg gctgagcgtc aacggcggcg agggtgacac
     9181 gctggcgaaa atgcttttcg gttatggact gctgcaactg ctgtttatgc tacgtctgat
     9241 gccatggtat ctctcccagc catttaatgc ttcattctgg agtttctcgt tcggcgtatc
     9301 tgcactggca accaccggtt tgcatctggg gagtggcagc gataatggat ttttccatac
     9361 gctggcggtg ccgctgttta tctttaccaa ttttattatt gcaatactgc tcatccgtac
     9421 ttttgcgctt ctgatgcagg gaaaattgtt agtcagaacc gagcgcgccg ttttaatgaa
     9481 agcagaggac aaagaatgat cattcgtgac gaaaactatt ttactgataa atatgaatta
     9541 acccgcacac actctgaagt actggaagcg gtgaaagtgg ttaaaccggg taaaacgctg
     9601 gatctgggct gtggcaatgg tcgtaacagt ctttacctgg cagccaatgg ttatgatgtt
     9661 gacgcatggg ataaaaatgc catgagtatc gccaacgtcg agcgcattaa atccattgaa
     9721 aatctggata atttacacac ccgggtcgtt gatctgaata acctcacatt tgatggacag
     9781 tacgatttta ttctttcgac tgtggtgctg atgttccttg aggctaaaac catcccaggg
     9841 ttgattgcca atatgcaacg ttgcactaaa cctggtggtt acaacctgat tgtggcggcg
     9901 atggataccg ctgattatcc atgtaccgtc ggcttcacgt ttgccttcaa agagggagaa
     9961 ttacgtcgat attacgaagg ctgggagagg gtgaaataca atgaagacgt cggcgagctg
    10021 caccgcaccg acgccaacgg taatcgtatt aaactgcgtt tcgccacgat gctggcacgt
    10081 aaaaaatgac ccggtaagca caaaacgcgt gaaaattccc cacgctgaga tgatttactg
    10141 ttcttctttt cggtaagcat attttttatc gaagggatgt gaaattaatc acagtagtcg
    10201 aagtttttta gcagcttaac ttactgaaat ttaactactg atgattgact tagccccttt
    10261 ttcggcattg actatgtcgt ctgaaaaggg gctgaaaaat ttattttcac caacactttt
    10321 tttgccacaa cacgaagcgg cgctttttgc tataacttag aaagtaatat aatcatctca
    10381 ggaaactatt catgcgtacc acatcatttg cgaaagttgc agctttatgc ggcttgttgg
    10441 ctctgtctgg ttgtgcctct aaaatcaccc agccagataa atattctggt tttttaaaca
    10501 attactctga tttaaaagaa acaacctcag caacaggtaa acctgtttta cgttgggtag
    10561 acccgagttt tgatcaaagc aaatatgaca gcatcgtctg gaacccaatc acttattatc
    10621 cggtaccaaa accttctacc caggtagggc agaaagttct ggataaaatt ttgaactata
    10681 ccaacaccga aatgaaagaa gcgatagcgc agcgtaaacc agtggttacc accgctgggc
    10741 cgcgtagtct gattttccgt ggggccatta ccggtgtaga taccagcaaa gaagggctgc
    10801 aattctatga agtggttcct gttgcattag tggttgcggg gacgcaaatg gctacaggcc
    10861 accgtaccat ggatactcgc ctctattttg aaggtgagct gattgatgca gcgactaata
    10921 aaccggttat caaagtcgtt cgtcagggcg aaggtaaaga cctgaataac gaaagtacgc
    10981 caatggcttt cgaaaatatt aaacaagtta ttgatgacat ggcgaccgat gccaccatgt
    11041 ttgacgttaa caaaaagtag ttccagacgc gccatcgtta gatggcgctt tttatccggt
    11101 gcgccgtaaa accccatcct tcagggcggg gatataaggc gcggttttcc acctgaccag
    11161 gtgtttgctg ttgttcaatg tattggcgga tgacggatat tggcgcacct ccgcagctac
    11221 tggcaaaata gccaggactc cacaaaacgc ctttgtagta atacctgact gcaatatctg
    11281 gtcgatctcg tcgcagtaac ctacccgata cgcctttgag gctgtttacc agactggata
    11341 tcgccagttt gggagg
//
LOCUS       DAJVRL010000113        10926 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVRL010000113 DAJVRL010000000
VERSION     DAJVRL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.113"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            154..504
                     /locus_tag="OQN02_003287"
     CDS             154..504
                     /locus_tag="OQN02_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS1643974.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="OQN02_003288"
     CDS             557..952
                     /locus_tag="OQN02_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1643975.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="OQN02_003289"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="OQN02_003289"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1643976.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="OQN02_003290"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="OQN02_003290"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1643977.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OQN02_003291"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="OQN02_003291"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS1643978.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="OQN02_003292"
     CDS             complement(5323..6423)
                     /locus_tag="OQN02_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1643979.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OQN02_003293"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="OQN02_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1643980.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="OQN02_003294"
     CDS             complement(7571..8137)
                     /locus_tag="OQN02_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1643981.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="OQN02_003295"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="OQN02_003295"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1643982.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="OQN02_003296"
     CDS             10263..10751
                     /locus_tag="OQN02_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCS1643983.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2670 a         2788 c         2605 g         2863 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 tatagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DAJVRL010000114        10925 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVRL010000114 DAJVRL010000000
VERSION     DAJVRL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.114"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..203
                     /locus_tag="OQN02_003297"
     CDS             <1..203
                     /locus_tag="OQN02_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1643984.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="OQN02_003298"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="OQN02_003298"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS1643985.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="OQN02_003299"
     CDS             2102..2818
                     /locus_tag="OQN02_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1643986.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="OQN02_003300"
     tRNA            complement(3274..3349)
                     /locus_tag="OQN02_003300"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OQN02_003301"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OQN02_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="OQN02_003302"
     tRNA            5082..5157
                     /locus_tag="OQN02_003302"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OQN02_003303"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OQN02_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCS1643987.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OQN02_003304"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OQN02_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS1643988.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="OQN02_003305"
     tRNA            7492..7567
                     /locus_tag="OQN02_003305"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OQN02_003306"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OQN02_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1643989.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="OQN02_003307"
     CDS             complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="OQN02_003307"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1643990.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="OQN02_003308"
     CDS             complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="OQN02_003308"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1643991.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="OQN02_003309"
     CDS             complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="OQN02_003309"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCS1643992.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3035 a         2782 c         2364 g         2744 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcaggc
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaaatt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccac gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagataattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgctggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccgggtgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     9301 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     9361 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg
     9421 ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga ccccttcctc
     9481 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     9541 attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc ctaggctacc
     9601 aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg cagaattgat
     9661 ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct ttatagcagt
     9721 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gcccgtttgc
     9781 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     9841 atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc ggctaagccg
     9901 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     9961 tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
    10021 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaga
    10081 atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa acggctatca
    10141 cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc acaggtatca
    10201 gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac gctaaacagt
    10261 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
    10321 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ctcgaaatcc
    10381 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
    10441 aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
    10501 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
    10561 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
    10621 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
    10681 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
    10741 ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca tgaattaatt
    10801 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggctt tgccgatgat
    10861 gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata tacagaacct
    10921 gtgaa
//
LOCUS       DAJVRL010000115        10894 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVRL010000115 DAJVRL010000000
VERSION     DAJVRL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.115"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="OQN02_003310"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="OQN02_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1643993.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="OQN02_003311"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="OQN02_003311"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1643994.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="OQN02_003312"
     CDS             complement(2271..3686)
                     /locus_tag="OQN02_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1643995.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="OQN02_003313"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="OQN02_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1643996.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="OQN02_003314"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="OQN02_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10894)
                     /gene="mdtA"
                     /locus_tag="OQN02_003315"
     CDS             complement(9875..>10894)
                     /gene="mdtA"
                     /locus_tag="OQN02_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS1643997.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREYAK
                     KGARS"
BASE COUNT         2507 a         3070 c         3000 g         2317 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggt
//
LOCUS       DAJVRL010000116        10662 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVRL010000116 DAJVRL010000000
VERSION     DAJVRL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.116"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(51..1400)
                     /gene="lysC"
                     /locus_tag="OQN02_003316"
     CDS             complement(51..1400)
                     /gene="lysC"
                     /locus_tag="OQN02_003316"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1643998.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            1925..3574
                     /gene="pgi"
                     /locus_tag="OQN02_003317"
     CDS             1925..3574
                     /gene="pgi"
                     /locus_tag="OQN02_003317"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1643999.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4036..4278
                     /locus_tag="OQN02_003318"
     CDS             4036..4278
                     /locus_tag="OQN02_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644000.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4392..5030
                     /locus_tag="OQN02_003319"
     CDS             4392..5030
                     /locus_tag="OQN02_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1644001.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5027..5764
                     /locus_tag="OQN02_003320"
     CDS             5027..5764
                     /locus_tag="OQN02_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1644002.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5764..7860
                     /locus_tag="OQN02_003321"
     CDS             5764..7860
                     /locus_tag="OQN02_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1644003.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7907..8185)
                     /locus_tag="OQN02_003322"
     CDS             complement(7907..8185)
                     /locus_tag="OQN02_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644004.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8186..8329
                     /locus_tag="OQN02_003323"
     CDS             8186..8329
                     /locus_tag="OQN02_003323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644005.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8455..8865
                     /gene="psiE"
                     /locus_tag="OQN02_003324"
     CDS             8455..8865
                     /gene="psiE"
                     /locus_tag="OQN02_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1644006.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="OQN02_003325"
     CDS             complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="OQN02_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCS1644007.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2707 a         2722 c         2763 g         2470 t
ORIGIN      
        1 gacaagaaaa tcaatacggc ccgaaatata gcttccaggc catacagtat ttactcaaac
       61 aaattactat gcagtttttg caccacctgc tcggcatctt cgccgggcac caggaagcac
      121 aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg
      181 aatacctctt tgccaacgcc gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc
      241 gccagacctt cttccacctc cacccggcac agtgcggaaa gctccatcag cagagattgc
      301 gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat caagggttaa tgccacgctc
      361 acttctgacg tggtgattaa gtctaccgaa atattatgcc gcgcgaggat gccgaaaact
      421 tccgcgagga agccgcgaga atgcagcata ttcaggctgt gcaaagtgag cagagtctga
      481 ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt cagttttatt gcacaccagc
      541 gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga ccgggatatc gctgcgtact
      601 gcgggtagca acgttgccgg atgcagtact tttgcaccaa aagttgccat ctctgccgct
      661 tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg
      721 tagatgcccg ggacgtcggt ccagatatca acacgagatg cgtgtaaagc ctccgccagc
      781 aaggctgccg tataatcgct gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg
      841 ctaccgataa atccctgggt gatcactaag ccttcattga ggcgtgggag cagctgcagc
      901 gcggccagtt ccgccagcgc ggctacatct ggctctgcac gaccaaatcg gtcgttggta
      961 cgcatcactt tacgtacatc aaaccactgt gcctgaacat cgcgttcgcg caggatttcg
     1021 acaaacagca gggtcgacat cagctcgccg tggctgacca gctcatctgt cagcgccgga
     1081 gacgttgcca gcgccgccgc ttctgccaga acagtaatgt tctccagcag acgttcaatc
     1141 tcttcacgga taacgttcgg gtaacgcaga cgttccagaa tggcaaactg gatgttgcga
     1201 atagcgtcga gtttttcgaa tcgctcgcca agttccagcc cttccgctaa agcgaccagc
     1261 agattagtga taccagcaga agccgagagg actactaaac gcacgttggc atcagaaagc
     1321 acaatatcag cgctgcggtt catggcgtca aaatcagcta cgctggtacc gccaaatttg
     1381 gagacaacaa tttcagacat aactacctcg tgtcagggga tccattttca gccttggcac
     1441 aagggaagag cggaagacgg gtgggcgcag agcgatactt cgctactatt ttcacccaga
     1501 agtgctccac cacttgcgaa acgcccgact gcgaacgctt ctggtgacaa cccaggggat
     1561 tcagcccctg tagccgatga tgaacgtggc cagccgttca atcacctcgg cgatgcaccc
     1621 cctcaggtgt tatcacagga ctggctcctc caacaccgtt acttgggcaa cgcgcctctt
     1681 ctggcctgcg ctagcgcagg tagtacattt ataaataaag ggtgagcggg gcggttgtca
     1741 acgacggggt catgcggatt tttcatccac tcctggcggt cagtagttca gctaataaat
     1801 gcttcactgc gctaagggtt tacactcaac attacactaa cggcactaaa accatcacat
     1861 ttttctgtga ctggcgctac aatcttccaa agtcacaatt ctcaaaatca gaagagtatt
     1921 gctaatgaaa aacatcaatc caacgcagac cgctgcctgg caggcactac agaaacactt
     1981 cgatgaaatg aaagacgtta cgatcgccga tctttttgct aaagacggcg atcgtttttc
     2041 taagttctcc gcaaccttcg acgatcagat gctggtggat tactccaaaa accgcatcac
     2101 tgaagagacg ctggcgaaat tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat
     2161 taagtcgatg ttctctggcg agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt
     2221 agcgctgcgt aatcgtagca ataccccgat tctggttgat ggcaaagatg ttatgccaga
     2281 agtcaacgcg gtgctggaga agatgaaaac cttctcggaa gcgattatct ccggtgagtg
     2341 gaagggttat accggcaaag caatcactga cgtagtgaac atcgggatcg gcggttctga
     2401 cctcggcccg tacatggtga ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca
     2461 ctttgtttct aacgtcgatg ggactcacat ctcggaagtg ctgaaaaaag taaacccgga
     2521 aaccacgctg ttcctggtag catctaaaac cttcaccact caggaaacca tgaccaacgc
     2581 ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca
     2641 ttttgcggcg ctctcaacca atgccaaagc cgttggcgag tttggtattg atactgccaa
     2701 catgttcgag ttctgggact gggtcggcgg ccgttactct ttgtggtcag cgattggcct
     2761 gtcgattgtt ctctccatcg gctttgataa cttcgttgaa ctgctttctg gcgcacacgc
     2821 aatggacaag catttctcca ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct
     2881 gattggcatc tggtacaaca atttctttgg tgcggaaact gaagcgattc tgccgtatga
     2941 ccagtatatg caccgtttcg cggcgtactt ccagcagggc aatatggagt ccaacggtaa
     3001 gtatgttgac cgtaacggta acgttgtgga ttaccagact ggcccgatta tctggggtga
     3061 accaggcact aacggtcagc acgcgttcta ccagctgatc caccagggaa ccaaaatggt
     3121 accgtgcgat ttcatcgccc cggctatcac ccataacccg ctctctgatc accaccagaa
     3181 actgctgtct aacttcttcg cccagaccga agcgctggcg tttggtaaat cccgcgaagt
     3241 ggttgagcag gaatatcgcg atcagggtaa agatccggca acgcttgact acgtggtgcc
     3301 gttcaaagtg ttcgaaggta accgcccgac caactccatc ctgctgcgcg aaatcactcc
     3361 gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa atctttactc agggcgtgat
     3421 cctgaacatc ttcaccttcg accagtgggg cgtggagctg ggtaaacaac tggcaaaccg
     3481 tattctgcca gagctgaaag atgataaaga aatcagcagc cacgatagct cgaccaatgg
     3541 tctgattaac cgctataaag cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat
     3601 gtaggccgga taaggcgttt acgccgcatc cggcaaccgg agcctgatgc caatatcgac
     3661 attaatgcat agtatgagga gttaaataac cttaaaaaca atgccgacaa taagtcggca
     3721 ttgttttatc agataaatcc ccttgtctgt gatttaacgg aaatcatacc gtgaggttaa
     3781 tcctaaaata gatttttaat cgttgtttat ttcggagaat acgcagatta attacttttg
     3841 tttttatttt aagcttatga tttttattgt tatttaaata taagttgaaa cttatatttg
     3901 atattcattc caattatcct aaaactccat cgctaattcc ccgcgccgta attcgcatgc
     3961 tttagttgtg tatactcgat cccgcccgaa atgtttttgg gtaaatctcc attcattcaa
     4021 tgaagggaaa ttgttatgaa aaaagttctg tatggcattt ttgccatatc tgcgcttgcg
     4081 gcgacttctg cgtgggctgc acctgtacag gtgggcgaag cggcagagtc ggcagcaacg
     4141 tcggtttcgg cggggagttc ctccgcgacc agcgtcagca ccgtaagctc ggcggtgggt
     4201 gtcgcgctcg cggcaaccgg tggcggtgat ggttctaata ccgggaccat cacaaccacg
     4261 accaccagta cccagtaata aagtatgtat ccccaaaata atttgagtca ttgcatctgt
     4321 ggcttgaagt atgaagggat ttaccataac cacactccgg tgtggttatt ctgcccctct
     4381 ggagaagagt cgtgaagcga cctgcactca ttcttatctg cctgctatta caggcctgtt
     4441 cagccacgac taaagagctg ggcaattcac tgtgggacag tctgttcggc acgccaggcg
     4501 tacagctgac ggacgatgat attcaaaata tgccctacgc cagccagtac atgcagctta
     4561 atggcgggcc gcaattgttt gtggtgctgg ccttcgctga agacggacaa caaaaatggg
     4621 tcactcagga tcaagccact cttgtcacac aacatggtcg tctggtgaag actttgcttg
     4681 gcggcgacaa cctgattgaa gtgaataacc ttgccgccga cccgctgatt aaacccgcac
     4741 aaattgttga tggtgcaaca tggacgcgca cgatgggctg gaccgagtac cagcaggtac
     4801 gctacgccac cgcacgctca gtcttcaaat gggatggcac cgataccgtc aaggtcggca
     4861 gcgatgaaac cctggttcgc gtgctggacg aagaagtctc caccgaccag gcgcgctggc
     4921 ataaccgcta ctggatcgac agcgaagggc aaattcgcca gtcggaacag tatctcggcg
     4981 cggattattt cccggtgaaa accactctca tcaaggcggc aaaacaatga ttaaacaaac
     5041 tattgtcgcg ttgcttttga gtgtgggagc gtcatcggtc ttcgcggcag gaaccgtcaa
     5101 ggtgttcagc aatggcagca gtgaggccaa aacgctaacg ggcgcagagc atttaatcga
     5161 tctggtaggg caacctcgac tggcaaacag ctggtggcct ggtgcggtga ttagcgaaga
     5221 gctggcaacg gcggcagcat tgcgtcagca gcaggcgttg ctgacacggc tggcagaaca
     5281 gggcgcagat tccagcaccg acgatgccgc cgccattaac gccttacgcc agcaaatcca
     5341 ggcattgaaa gtgacaggca ggcaaaaaat caatcttgat cccgatatcg tccgcgttgc
     5401 cgaacgcggt aacccgccgt tgcagggcaa ctacacgctg tgggtcggac caccgccgtc
     5461 cacggtcacg ttgttcgggc ttatcagcca tcctggcaat cagccattca ctccaggtcg
     5521 cgacgtggcg agctatctct ctgaccagag cctgctcagc ggtgcggatc gcagctacgc
     5581 gtgggtcgtt tacccggacg gacgcacgca aaaagcgccg gtggcttact ggaacaagcg
     5641 tcacgtagag ccgatgcccg gcagcattat ttatgttggc ctcgcagatt ccgtctggag
     5701 tgagacgcct gatgccctta acgccgacat tcttcagact ctgacgcagc ggatacccca
     5761 ataatgaaaa aaagacatct gcttagctta ctggcgctgg gcattagcac agcttgctac
     5821 ggcgaaacat atcctgcgcc cattggtccg tcgcagtcgg atttcggtgg cgtaggatta
     5881 ttacaaactc ccaccgcgcg tatggcacgg gaaggggagt tgagtctgaa ctatcgcgat
     5941 aacgatcagt accgttatta ctcagcttca gtgcaactct tcccgtggct ggaaacaacg
     6001 ctgcgctaca ccgacgtgcg cacccggcag tacagcagcg tcgaagcgtt ctctggcgat
     6061 caaacgtata aagataaagc cttcgatctc aaactgcgtt tgtgggaaga gagttactgg
     6121 ctgccgcaag tggcggttgg tgcgcgggat atcggcggta cggggctgtt tgatgcggaa
     6181 tatcttgttg ccagcaaagc ctgggggccg ttcgatttta cgctcggcct gggctgggga
     6241 tatctgggca ccagcggtaa tgtaaaaaat ccgctctgtt cagccagtga taaatattgc
     6301 tatcgcgata acagctacaa acaggcggga tctatcgacg gtagccagat gttccacggt
     6361 cctgcctcac tgtttggcgg cgtggaatac cagacgccct ggcaaccgct gcgtctgaaa
     6421 ctggagtatg aaggtaataa ttatcagcag gattttgccg ggaagctgga gcaaaaaagt
     6481 aagtttaacg tcggggcgat ttatcgcgtt accgattggg ccgacgttaa ccttagctat
     6541 gaacgtggca acacctttat gtttggcgtc acgctgcgca ccaactttaa cgatctgcgc
     6601 ccgtcctaca tcgataacgc ccgcccgcaa tatcaaccgc agccgcagga tgccattttg
     6661 cagcattcgg tagtggcgaa tcagttaacg ttgctgaaat acaacgccgg acttgccgat
     6721 ccgcagatcc aggcgaaagg cgatacgctg tatgttaccg gcgagcaggt gaaatatcgt
     6781 gattcgcgcg aagggatcat ccgtgctaat cggatcgtga tgaacgatct gccggatggg
     6841 atcaaaacga tccgcattac ggaaaatcgc cttaacatgc cgcaagtgac gacggaaacc
     6901 gatgtcgcca gcctgaaaaa tcatctcgcc ggagagccgt tgggccacga aacgacgctg
     6961 gcgcacaaac gcgtcgagcc agtggttccg aagtccaccg agcagggctg gtatatcgac
     7021 aaatcacgct ttgatttcca tatcgatccg gtgctgaacc agtcggtcgg tggcccggaa
     7081 aacttttaca tgtatcagct gggcgtgatg ggaacggcag atttgtggct gacggaccat
     7141 ctactgacca ccggcagcct gtttgcaaat cttgccaaca actacgacaa gtttaactac
     7201 accaatccac cgcaggactc gcacttaccg cgcgttcgta cccatgtgcg cgagtatgtg
     7261 cagaacgatg tctatgtgaa taacctgcaa gccaactact tccagcatct gggcaacggc
     7321 ttctacggtc aggtctatgg aggttatctc gaaaccatgt ttggcggtgc gggggccgaa
     7381 gtgttgtatc gtccgctgga cagtaactgg gcgtttggtc tggatgccaa ctacgttaaa
     7441 cagcgcgact ggcgtagtgc aaaagatatg atgaaattca ccgactacag cgtgaaaacc
     7501 ggacatctga ccgcctactg gacgccatct ttcgcccagg acgtgttggt gaaagccagc
     7561 gtcgggcagt atctggcagg ggataaaggc ggcacgctgg agatcgctaa acgctttgac
     7621 agcggcgtgg tggtgggtgg ctatgccacg atcaccaacg tttcgaaaga ggagtacggc
     7681 gaaggggact tcaccaaagg cgtgtatgtc tcggtaccgt tggatctctt ctcgtctggc
     7741 ccgacacgca gccgtgcggc gattggctgg acgccgctga cgcgtgacgg tggtcagcaa
     7801 cttgggcgta agttccagtt gtatgacatg accagcgacc gtagcgtaaa tttccgctaa
     7861 gtcatgggaa aggtgccagt tttcgcactc actggcaccc tcattcttaa tgacctgttg
     7921 agccatcagg aacgcagttc acccccggtt tacgatctgg tgtgtaaagg cgaaaacatt
     7981 ctgtcttcct gtccgtcgat atcggttgca ggtctggttg cctgagcgac tgggccatca
     8041 ttgggctgga gacattgctt tgcggtaggt cggctttatc agcagtaccg agcggaccag
     8101 cataagcagg aagaacagag actgataaca tcaaagcagc aaaataaggc ttcattttta
     8161 ccacctttat caggttacgt ttcatttgtt ccagaggaac attgtcgatt tttcgcgcat
     8221 tgctggtggc tgggaatcac ctgaatgggt gatttttgaa ttaccggctt tggtgcggtt
     8281 tgtcttgccg gatgcgccgc caggcgcggc ttatccggcc tacgggtagg tatatccggc
     8341 tttggtggag gcgcgctcca aatccaggtt gaacaaaaca tacacaaaaa atatagatct
     8401 ccgtcacatt tttgcgttat acaggaagct cgccactgtg aaggaggtac tgctatgacg
     8461 tcactctctc gtccgcgcgt ggagtttatc tccaccattt tgcagaccgt actcaatctt
     8521 ggcctgctgt gcctcggcct gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg
     8581 gctgatgtgc tgttcgcgcc agaacaaacc agcaaatatg agctggtaga aggactggtg
     8641 gtttactttc tctatttcga atttatcgcg ctgattgtga agtactttca gtccggtttt
     8701 cacttcccgc tacgttactt tgtctatatt gggatcaccg ccattgtgcg cttgatcatc
     8761 gtcgatcata aatcgccact ggatgtgttg atctactcgg ctgcgatcct gttactggtg
     8821 atcaccctgt ggctgtgtaa ttcgaaacgg ctgaagcggg agtaaaaaga cagcacgccg
     8881 aaatggcgcg gcgtgctgga caggaagatt acagcgtagc agtttgttgt gttttcttcg
     8941 tttccggttc ccagagcgct tccagctcct caagggtttt acctttggtt tccgggacaa
     9001 atttccacat aaacagtgct gccagaacgc ccatacaacc gtaaatccag taggagaaac
     9061 cgttgtggaa atgggccacc agccaggagt ttttgtccat catcgggaag gtccaggaga
     9121 cgaagtagtt cgccagccac tgggccgcca ccgcgattgc cagcgcttta ccacgaatag
     9181 cattcgggaa gatttccgac agcagtaccc agcataccgg accccaggac atggcaaagg
     9241 cggcaacata gaacagcatc gacagtagcg ccacaatacc cgatgcctga gtgtaaaacg
     9301 cggtaccgag gctaaacata ccgattgcca ttccgagtgc gccgataatt tgcagtggct
     9361 tacgaccaaa tttatccaca gtcataattg ccagtacggt aaaggtgagg ttgataactc
     9421 cgacaataat ggtctgcaac agcgcgatat ccgtgctggc ccccagcgtt ttgaacactt
     9481 ccggcgcgta gtacagcacc acattgatgc cgacaaattg ctggaagatg gagagcataa
     9541 cgccgattac aatcacgccc acgccaaaca tcagcagacg accaccggtt ttgcggccat
     9601 gatccaggga gtgtttaatt tcctgtactg cctgagttgc aagcgtgttg cccataattt
     9661 tgcgcaggat actttccgtc tgttcttgct tgccgcgtga catcagccag cgaggacttt
     9721 ctggcacggt atacagcagc attaagaaca gcagtgcagg gatacattcc gaggcaaaca
     9781 tataacgcct gccgtcagta ttcagccagc tggcatcacc ggaacgggca ataaaatagt
     9841 ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa ctggttaaat gaaaccagtt
     9901 ttccgcgaat atgagctgga gccagttccg caatatacat tggtgaaagc attgaggcta
     9961 aaccaacgcc aataccgcca ataatacgat aaataacaaa ttccgggaca taacctgcca
    10021 gataaatagg tactgtgttg tccgggttta tagaggtaaa accaagttct ggccaggcag
    10081 aacctacacc agaaataaaa aacaggacag cagaaatctt aagtgaatca cgacggccga
    10141 agcggttact gcaataacca ccgagggcac cgccgatgat gcaaccaatc agagcgctgg
    10201 ctacgcaaaa tcctaacagg gagttggcag cggattcact taagttttgt ggagcaacaa
    10261 agacggtatt gagtgactca acagtaccgg aaataacggc ggtgtcgtag ccaaataata
    10321 aaccacctaa tgtagcgact aaggtaatcg aaaatatata actggaatta tactgggtat
    10381 tcattcagac ctgccttaga ccattctgat cttttatcag tgcattgatg ttggaattgt
    10441 caggcagtta aggcaattat cattttttac aatgcgttta cgtaatgtct tttctgtgat
    10501 cttaattgtg ataattatca aaaattgatg aaaacaatac tattgccgtg actcagagca
    10561 cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa aaataataag ttctatgatg
    10621 ggaatattaa ccggcgaacg attcagattg cagacgaaag aa
//
LOCUS       DAJVRL010000117        10629 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVRL010000117 DAJVRL010000000
VERSION     DAJVRL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.117"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            51..1541
                     /locus_tag="OQN02_003326"
     CDS             51..1541
                     /locus_tag="OQN02_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1644008.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="OQN02_003327"
     CDS             1593..2750
                     /locus_tag="OQN02_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1644009.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="OQN02_003328"
     CDS             complement(2716..2838)
                     /locus_tag="OQN02_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644010.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="OQN02_003329"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="OQN02_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="OQN02_003330"
     CDS             4764..7124
                     /locus_tag="OQN02_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1644011.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQETRKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7181..9964
                     /locus_tag="OQN02_003331"
     CDS             7181..9964
                     /locus_tag="OQN02_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1644012.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10325..>10629
                     /locus_tag="OQN02_003332"
     CDS             10325..>10629
                     /locus_tag="OQN02_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1644013.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         3151 a         2343 c         2296 g         2839 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaaacgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7381 agaagacaat gagctcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7441 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7501 cgatgttaat gcctatatca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7561 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7621 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7681 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7741 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7801 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7861 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     7921 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     7981 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8041 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8101 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8161 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8221 tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt tggcaaccat
     8281 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8341 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcgcct
     8401 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8461 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8521 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8581 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8641 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8701 tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8761 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8821 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     8881 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     8941 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9001 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9061 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9121 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9181 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9241 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9301 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9361 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9421 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9481 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9541 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9601 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9661 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9721 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9781 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9841 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
     9901 tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa
     9961 ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta
    10021 tgtaataatt ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat
    10081 gcctgatttt tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa
    10141 acacctttcc accacccaag aattctctat tacattgttt ttaatgaaaa aatatcattc
    10201 caacaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
    10261 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
    10321 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
    10381 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
    10441 cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
    10501 cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc
    10561 cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
    10621 ttgcgtaaa
//
LOCUS       DAJVRL010000118        10487 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVRL010000118 DAJVRL010000000
VERSION     DAJVRL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.118"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(24..1052)
                     /gene="malI"
                     /locus_tag="OQN02_003333"
     CDS             complement(24..1052)
                     /gene="malI"
                     /locus_tag="OQN02_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1644014.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1227..2819
                     /gene="malX"
                     /locus_tag="OQN02_003334"
     CDS             1227..2819
                     /gene="malX"
                     /locus_tag="OQN02_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1644015.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2829..4001
                     /gene="malY"
                     /locus_tag="OQN02_003335"
     CDS             2829..4001
                     /gene="malY"
                     /locus_tag="OQN02_003335"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1644016.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4105..5106
                     /gene="add"
                     /locus_tag="OQN02_003336"
     CDS             4105..5106
                     /gene="add"
                     /locus_tag="OQN02_003336"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1644017.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5142..6182)
                     /locus_tag="OQN02_003337"
     CDS             complement(5142..6182)
                     /locus_tag="OQN02_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1644018.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6425..6550
                     /gene="blr"
                     /locus_tag="OQN02_003338"
     CDS             6425..6550
                     /gene="blr"
                     /locus_tag="OQN02_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1644019.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6823..7038
                     /gene="ydgT"
                     /locus_tag="OQN02_003339"
     CDS             6823..7038
                     /gene="ydgT"
                     /locus_tag="OQN02_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1644020.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7124..7564
                     /locus_tag="OQN02_003340"
     CDS             7124..7564
                     /locus_tag="OQN02_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1644021.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7641..8222
                     /gene="rsxA"
                     /locus_tag="OQN02_003341"
     CDS             7641..8222
                     /gene="rsxA"
                     /locus_tag="OQN02_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1644022.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8222..8800
                     /gene="rsxB"
                     /locus_tag="OQN02_003342"
     CDS             8222..8800
                     /gene="rsxB"
                     /locus_tag="OQN02_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1644023.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8793..>10487
                     /gene="rsxC"
                     /locus_tag="OQN02_003343"
     CDS             8793..>10487
                     /gene="rsxC"
                     /locus_tag="OQN02_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1644024.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2405 a         2708 c         2670 g         2704 t
ORIGIN      
        1 aagtatagca gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga
       61 ttgcgtgaat gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtgata
      121 ccaagttccc gcgctggcgt gctggcccag gtaacaggta tatcatcaag tgtggttggt
      181 gtcgcatcgg taaatgccgc cagcgaaacc tgttgctcaa agtaacgatc gactccgctt
      241 tccccgcttt gccgccctgc tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg
      301 ttatagcaaa ccacggcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc
      361 gcggcttgct tctggctgga agtgcactcc aacacccaat cgctgtgaaa cggcaggcca
      421 aattttagta gagttgcaca atagcccccc acccgttctg cacgggttaa tgaggaactt
      481 tgccctccca gccaggcgat ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac
      541 tgtgcagcct gcatgttgtc cgggcgaacc gtatcaacat catcaagata actggcacgg
      601 gaagcgaaaa tcaccgggat agctttttct tctgccatcc gccgcaggtc atcgctgctt
      661 cctgcagccc cggcaattac cacaccatcg acaccctgat tcagtaacag tgaaaaccgc
      721 tgtgccagct gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgtgct
      781 tccagagctt ccgtcaatcc ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg
      841 acgatcaaac caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg tcgattgcgc
      901 acaaatccca gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc
      961 cctttgccat taagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca
     1021 tcatgaatgg ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga
     1081 cagataaaac gttttacctt ttatttatct tatacccgct attatcgttg cgtaatgtga
     1141 tttatgactc actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca
     1201 gattgttctc tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt
     1261 gggagttctt ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct
     1321 gcggcattat gctcggcatt ggtagttctc ttagcagcca tgatgtcata accctgatcc
     1381 cggtcctggg caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt
     1441 ttgcttttag tttcctgcct gtcatgttct gtatcgccat cccgctgggc ctggcacgcg
     1501 aaaataaagg cgtagcggca ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg
     1561 taaacttctg gttgaccaat aaaggcattc tgccaaccac agatgccgcc gttctgaaag
     1621 ccaataacat ccagagcatt cttgggatcc agtcgatcga taccgggatc ctcggtgcgg
     1681 tgatcgccgg tattatcgtc tggatgctgc atgagcgttt ccataatatc cgcctgccgg
     1741 atgcgctggc attcttcggc ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg
     1801 gccttgtcgg cctggtgatt ccattagtct ggccgatttt cgccatgggt attagcggct
     1861 tgggccatat gataaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac
     1921 gtctgctgtt gccgtttggt ctgcatcaca ttctggtggc attaattcgc tttaccgacg
     1981 caggcggcac gcaggaggtc tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg
     2041 cgcaattgag ttgcccgacc actcacggtt tttctgaaag cgtcacgcgt ttcctttcgc
     2101 aaggtaaaat gcctgcgttt ctcggcggtc taccaggtgc agcgttagct atgtatcact
     2161 gcgcgcgccc ggaaaatcgc cataaaatta aaggtctgct gatttctggc ctgatcgcct
     2221 gcgtcgttgg cggcactacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc
     2281 tgtatgtcat ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg
     2341 tcaccatcgg taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg
     2401 gtctgtcaac caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact
     2461 acgtcatctt ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg
     2521 aagttgccag ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg
     2581 ttcctgcaat cctcgaagca ttaggcggtg ccgacaatat tgtcagcctc gataactgca
     2641 ttacccgtct gcgtttgtct gtggaagata tgtcgcttgt taatgtgcag gcactgaagg
     2701 acaatcgggc aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc
     2761 cacaagttca gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag
     2821 gataagatat gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt
     2881 gggattatgt cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca
     2941 tggattttgc cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg
     3001 tatttggcta cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt
     3061 ccacccagca ttacaccgca attgatacgc agacggtggt gtatggccct tctgtcatct
     3121 atatggtttc agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca
     3181 cgcccgccta tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg
     3241 ttgctttaga gaagcaggct gacggctggt tttgcgatat gggcaagctg gaagccgttt
     3301 tggcgaaacc agaatgtaaa attatgctcc tgtgtaaccc acagaatcct accgggaaag
     3361 tgtggacgtg cgatgagctg gagatcatgg ctgacctgtg cgagcgtcat gatgtgcggg
     3421 ttatttccga tgaaatccat atggatatgg tctggggcga gcagccgcat attccctgga
     3481 gtaatgtggc gcggggagac tgggcgttgc tgacatcggg atcgaaaagt ttcaatattc
     3541 ccgcgctgac cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat
     3601 cggcactgaa aggacgtgat gggctttctt ccccttcggt actggcgtta actgcccata
     3661 tcgccgccta tcagcaaggt gcaccgtggc tggatgcctt acgcgtctat ctgaaagata
     3721 acctgacgta tatctcagat aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc
     3781 cgcaatccac ttatctggcc tggcttgatc tacgtccgtt gaatattgac gacaacgcgt
     3841 tgcaaaaagc gcttatcgag caagaaaaag tcgcgatcat gccggggtat acctatggtg
     3901 aagaaggtcg tggttttgtc cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag
     3961 gtgtggctgg attaataaac gccatccgcg ctgttcgcta accccaattg cgcaacgtaa
     4021 aaaatcgttg cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt
     4081 tatatccaga aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat
     4141 cgccaccttg atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat
     4201 atctcgcttc ctgcacaatc ccttgaaaca ctgattcccc acgttcaggt cattgccaac
     4261 gaacccgatc tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct
     4321 cttgatgcct gccgccgcgt ggcatttgaa aacattgaag atgcagcccg taacggcctg
     4381 cgctatgtcg agctgcgttt ttcaccaggc tacatggcaa tggcacataa gctgcctgta
     4441 gcgggtgttg tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg
     4501 caggcgaagc ttatcggcat tatgagccga accttcggcg aagccgcctg tcagcaagag
     4561 ctggaggcct ttttagccca ccgtgaccag attaccgcac ttgatttagc tggtgatgaa
     4621 cttggattcc cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg
     4681 catattaccg tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt
     4741 gaactgggtg cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg
     4801 gattttctcg ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc
     4861 agcaccgtag cagagctggc tgcacatccg ctgaaaacgt tccttgagca tggcattcgt
     4921 gccagcatta acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc
     4981 gttgccgcgc cagctgctgg gttatcccgt gagcaaatcc gccaggcaca gattaatggt
     5041 ctggaaatgg ctttcctcag cgcagaggaa aaacgcgcac tgcgagaaaa agtcgccgcg
     5101 aagtaacaaa aatggatggt gcaaattgca ccatccattt ttcacgcaag gcacaacgtc
     5161 gcgcgatgtt tggcggattc gatgcccagc tcaatcaact ccattacctg gattgcctgg
     5221 cttgccggaa ccggattttc accatcgcca tttaacgcat cacgaatcgc cgcatagtaa
     5281 gccggataat tcccaggcac cgtcaacagc gtttcttcga cacgttcctc accttccacg
     5341 cgggtaagta cgccatcacg catatcgtag ccccagtctt cctgcggcag acgctcgcca
     5401 tttttcagac gttcttcctg tggatcgagg ccatatttca cataactgcc tcgggatcca
     5461 tgcacgatat accgtgctga ctcagcggct gccagcatgg taccgtgtaa aatgactcgc
     5521 cgctgtggat aggacaagat ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac
     5581 tgtgccaaat ctaccgtcat gctgaccggt aaaccaaata gcgtaatggc ctgatcaaga
     5641 agatgcggtg ctaaatcgta ccagataccg ctgcctgggc cgccctgttc acgccaacga
     5701 tcgcgcacct gcggacggaa gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc
     5761 acgccttccg cgagcaaacc ttttagcgtc aagaaatcgc tatcccaacg acggttatgg
     5821 aatacagaca gcacacgccc caggcttttt gccagcgcat ccagctctcg cgcttgtgac
     5881 agtgtcacgg taaagggttt atcaacgacc acatatttac ccgcctcaag cgccgctttg
     5941 gctaacggga aatgggtatc gttgggtgta ggaatgacaa tcaggtctat gttgggatcg
     6001 ttaaacagat gcttcggctc agagacaacc gtaaccgttg gccagtcggc ttttactttt
     6061 gtttcatcac tgctggagat taccgccagt tccagcccgg gcgtgcccgc aatcaggggc
     6121 gcatggaagg ttttgctcgc ataaccatac ccaatcaacc caacacggat gttgtcgctc
     6181 atgtcattgc ctctcattga agtatgatgg ctatttgaca ctatccttta cccacgctca
     6241 acagtttaat aacctgccag caataaggga tgttgtttaa cttaagtcaa aaaaatagcg
     6301 aattttccaa cgacaaaagc taaatatcgc aaaaacctca gtaaaaatct tgctggagct
     6361 attattgcta agtaacattt accccctgaa gttaatggat caatcaagag agatgtgggc
     6421 tgtaatgaat cgtcttattg aattaacagg ttggatcgtt cttgtcgttt cagtcattct
     6481 tcttggcgtg gcgagtcaca ttgacaacta tcagccacct gaacagagtg cttcggtaca
     6541 acacaagtaa gctctgcact tgtggagcga catgctgccc gtccgggtgc atgttttcac
     6601 ttgtcggata ttaaaccagg aatttattat cttgttcgat gttgttggtg attgtcaggg
     6661 atagtaaagc gcgttactct tttcagagca gtttttcatc cctgaatatt tgccatcagc
     6721 gcattcttat tattgtaata acacaagcgt tcacttttgg tgaaatttta tcatcactaa
     6781 taatacctac ccctttggta ttaattattt tatggacctt ttatgactgt tcaggactac
     6841 ttattaaaat ttcgcaaaat cagttcactc gaaagtctgg aaaaactcta cgaccatctt
     6901 aattacaccc tgacggacga tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt
     6961 cgcgcagagc tggtttctgg cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc
     7021 tggcactatg tccaataaag aaagtagcga tcggttataa aagccttata ataacgcccc
     7081 taaaattaga tttgcctgtt tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc
     7141 acaacgtatt ggaggctggt tgcttggccc gttggcctgg cttttagtcg ctctgttgag
     7201 tacgacgctg gcgttgttgc tgtataccgc tgcgttatct tctccgcaaa catttcaaac
     7261 gctcggcgga caagcactga cgacacaaat cttatggggc gtttctttta ttaccgctat
     7321 cgccatgtgg tattacacac tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt
     7381 tcctaaacac tatattatct ggctgcttat ttccgtattg ctggcagtaa aagcattcgc
     7441 tttttcaccc gtggaagttg gcatagccgt tcgccagttg ctgtttactt tgttggcaac
     7501 cgcattgatc gtcccctatt tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc
     7561 gtaataacct tacagttaac ctgttgtcgc ctgctctgga ttaacggata ataggcggct
     7621 tttttatttc aggccgaaaa atgactgact acctgttact gtttgtcgga actgtactgg
     7681 tcaataactt tgtactggtc aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa
     7741 aactggaaac cgcgatgggc atggggctgg caacaacgtt tgtgatgacg ctgacgtcta
     7801 tttgcgcctg gcttatcgat acgtggattt tgatcccact taatctgatt tacctgcgca
     7861 ccctggcatt tattctggtg attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca
     7921 aaaccagccc ggtgctttac cgtctgctgg ggattttttt gccgcttatc accaccaact
     7981 gtgcagtgct cggcgtggcg ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg
     8041 cgctgtacgg tttttccgcc gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca
     8101 tccgcgaacg ccttgctgtg gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt
     8161 taattaccgc aggtcttatg tctctggcct ttatgggctt tagtggtttg gtgaagttgt
     8221 aatgaatgct atctggattg ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc
     8281 cattctgggt tatgcctccc gccgttttgc ggtggaagac gatccggtcg ttgagaaaat
     8341 tgacgaaatc ttaccgcaga gccagtgtgg tcagtgcggt tatcccggct gtcgccccta
     8401 cgcggaagcc atcagctgta acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc
     8461 tgtgatgcta aaaattgccg agttgcttaa tgtcgagccg cagccgctgg atggcgaagc
     8521 gcaagagcta acgcctgcgc ggatggtggc ggttattgat gaaaataact gtattggctg
     8581 cactaaatgt attcaggctt gtccggtaga cgccatcgtt ggcgctaccc gtgtcatgca
     8641 tacggtaatg agtgatctct gtacgggctg caatttatgt gttgatccgt gcccgacgca
     8701 ctgcatctcg ttgcaaccgg tcgcagaaac acctgactcc tggaaatggg atctgaacac
     8761 cattcccgtg cgtatcattc ccgtggaaca ccatgcttaa gttattctct gcattcagaa
     8821 aaaataaaat ctgggatttc aacggcggca tccatccacc ggagatgaaa acccagacca
     8881 acggtacccc cctgcgccag gtactactgg cgcagcattt tgttattcca ctgaaacagc
     8941 atattggcgc tgaaggtgag ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc
     9001 cgcttacccg tggtcgcggc aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta
     9061 cggctattgc gccccactct acggctcatc cttcagcttt agctgaatta agcgtgatta
     9121 ttgatgccga tggtgaagac tgctggatcc cgcgcgacgg ctgggtcgat tatcgctctc
     9181 gcagtcgcga agagttaatc gagcgcatac atcagtttgg tgttgccggg ctgggcggtg
     9241 ccggattccc gacaggcgtt aaattgcagg gtggtggaga taagattgaa acgttgatta
     9301 tcaacgctgc tgagtgcgag ccgtacatta ccgccgatga ccgtttgatg caggattgcg
     9361 cggctcaggt cgtagagggt attcgcattc ttgcgcatat tctgcagcca cgcgaaattc
     9421 ttatcggcat tgaagataac aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg
     9481 actctaacga tatttctctg cgggtgattc caaccaaata tccttctggc ggtgctaaac
     9541 aattaaccta cattctgacc gggaagcagg ttccacatgg cgggcgttca tccgatatcg
     9601 gcgtattaat gcaaaacgtc ggcactgctt atgcagtgaa acgtgccgtt attgatggcg
     9661 agccgattac cgagcgtgtt gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg
     9721 tctgggcacg gctggggacg ccagtgcgtc atttattgaa tgatgccgga ttctgcccct
     9781 ctgccgatca aatggtgatt atgggtggcc cgctaatggg ctttaccttg ccatggctgg
     9841 atgtcccggt cgtaaagatt accaactgtc tgttggctcc ctctgccaat gaacttggcg
     9901 aaccacagga agaacaaagc tgcatccggt gtagcgcctg tgctgacgcc tgccctgtgg
     9961 atcttttgcc gcaacagttg tactggttca gcaaaggtca gcaacacgat aaagctacca
    10021 cgcataacat tgccgactgc attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata
    10081 ttcccttggt gcaatatttc cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag
    10141 aaaagcgcgc cgcagaagcc aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg
    10201 aaaaagcgac tcgccttgaa cgacataaga gcgcagccgt tcaacctgca gccaaagata
    10261 aagatgcgat tgctgccgct ctggcgcggg tgaaagagaa acaggcccag gctacacagc
    10321 ctattgtgat taaagcgggc gaacgcccgg ataacagtgc aattattgca gaacgggaag
    10381 cccgtaaagc gcaagccaga gcgaaacagg cagaactgga gcaaactaac gacgcagcaa
    10441 ccgttgctga accacgtaaa actgccgttg aagcagctat cgcccgc
//
LOCUS       DAJVRL010000119        10055 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVRL010000119 DAJVRL010000000
VERSION     DAJVRL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.119"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            71..202
                     /locus_tag="OQN02_003344"
     CDS             71..202
                     /locus_tag="OQN02_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS1644025.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            462..1457
                     /gene="pgl"
                     /locus_tag="OQN02_003345"
     CDS             462..1457
                     /gene="pgl"
                     /locus_tag="OQN02_003345"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1644026.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1498..2451)
                     /locus_tag="OQN02_003346"
                     /pseudo
     CDS             complement(1498..2451)
                     /locus_tag="OQN02_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2635..3687
                     /locus_tag="OQN02_003347"
     CDS             2635..3687
                     /locus_tag="OQN02_003347"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCS1644027.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3763..5196
                     /locus_tag="OQN02_003348"
     CDS             3763..5196
                     /locus_tag="OQN02_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCS1644028.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5379..7559
                     /locus_tag="OQN02_003349"
     CDS             5379..7559
                     /locus_tag="OQN02_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1644029.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7700..8983)
                     /locus_tag="OQN02_003350"
     CDS             complement(7700..8983)
                     /locus_tag="OQN02_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1644030.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9118..9999)
                     /locus_tag="OQN02_003351"
     CDS             complement(9118..9999)
                     /locus_tag="OQN02_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1644031.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2438 a         2355 c         2547 g         2715 t
ORIGIN      
        1 catggaagga gatcacccaa ttctcccccc cgacttttga cgcagtgtct tgttaacagg
       61 gggattgacc atgcttattg gctatgtacg cgtatcaaca aatgaccaga ataccgatct
      121 acaacgtaat gcgtttattt gtgccggaag tgagttgatt gttgcgcgaa tgggaagttg
      181 gccagaagtt gttatggcat agtgtttacg gtcgttgata caggtaaaca aaagcgggga
      241 gagtcatact ccctgaatag tcgcgaaaat atctgtggca ttgtcaaatt cgtgtcggtg
      301 actaatattt tagtaatatg ccattcgaaa tggagtgcta acttattgat ttaattgatg
      361 tgcgatattg tattttatcg tgttttgttg tctgtttttt atccagtaag tgttgttata
      421 tgtatgaaag ataagggatt tatcattttt gaggtgtgtt catgaagcaa acagtttata
      481 tcgccagccc tgagagccag caaattcacg tctggaacct gaatcatgaa ggcgcactga
      541 cgctgacaca ggttgtcgat gtgccggggc aggtgcagcc gatggtggtc agcccggaca
      601 aacgttatct ctatgttggt gttcgccctg agtttcgcgt cctggcgtat cgtattgccc
      661 cggacgatgg cgcactgacc tttgccgcag agtctgtgct gccgggtagt ccgacgcata
      721 tttccaccga tcaccagggg cagtttgtct ttgtaggttc ttacaatgcg ggtaacgtga
      781 gcgtaacgcg tctggaagat ggcctgccag tgggcgtcgt cgatgtagtc gaggggctgg
      841 acggttgcca ttccgccaat atctcaccgg acaaccgtac gctgtgggtt ccggcattaa
      901 agcaggatcg catttgcctg tttacggtca gcgatgatgg tcatctggtg gcgcaggacc
      961 ctgcggaagt gactaccgtt gaaggggccg gcccgcgtca tatggtattc catccaaacg
     1021 aacaatatgc gtattgcgtc aatgagttaa acagctcagt ggatgtctgg gaactgaaag
     1081 atccgcacgg caatatcgaa tgtgtccaga cgctggatat gatgccggaa aacttctccg
     1141 acacccgttg ggcggctgat attcatatca ccccggatgg tcgccattta tacgcctgcg
     1201 accgtaccgc cagtctgatt accgttttca gcgtttcgga agatggcagc gtgttgagta
     1261 aagaaggctt ccagccaacg gaaacccagc cgcgcggctt caatgttgat tacagcggca
     1321 agtatctgat tgccgctggg caaaaatctc accacatctc ggtatacgaa attgttggcg
     1381 agcaggggct actgcatgaa aaaggccgct atgcggtcgg gcagggacca atgtgggtgg
     1441 tggttaacgc acactaaccg ctgatttaac cggcgcagtc tctcctgcgc cggtgtatta
     1501 acttatctcc tgtaacgcat gtctctggcg ttcgacgata ttggtccaca aattgtcttt
     1561 atcgtcagtc cacaaattaa tcagcaagga aaaaaagcgt tcagctgccg gagaaagcac
     1621 ggcatctttg cggcgaataa tccccaatgt ccgacgtata acgggttcca ccagtgggat
     1681 accaataatc gaagaatagg gcgcatgggg cattgccaga ccaggaagcg ccgaaatccc
     1741 cagtcctgcc tccactagtc ctaatgacgt cgaaagatga cgcacttcgt aaaaccagtc
     1801 cagcttccag ggcttgtcgg ctagctgttg ttctatcaac aatcggttgc cgctggagga
     1861 acgtacgcca atcattttgt aacccactaa ctcctgccat tctacaagct actttttggc
     1921 caatggatgg tcacgccgac aggccagcac gaatggctcg ttgaccagtg gggtaaaatc
     1981 aatggatgaa tttgtgacgt tgttcatgtt tatgccaaag tctgattcgt tacacagcac
     2041 cgattccatg caattattcg tgccttgttc cagaatccgc actttaatat tgggatacag
     2101 ctcattaaat ttaccgattg ccagcggtaa aaaataaaat actgcggtcg gaatacacgc
     2161 taatgtcacc atatcacgat gataagcatt catatcacga atattaaaaa gcgtttcatc
     2221 gaatttcttt attaattccc gcgcctccgg gagcaacctt tttcctgctt ttgttaacgt
     2281 aaccttgcga gttgtacgct caaatagttg aatatgtaaa tcttcctcca tcttttttat
     2341 tcttcgcgtt aatgcaggtt gcgtaatatt gagtaattta gcggcattgt taaatgaact
     2401 ggactctgcc agtatgacaa atgccttcat acttgataat tcatgcttca ttattactcc
     2461 ggaaaatgga agcgacgatt ttggggggct ggtcgttaaa aattttaact gcatttagcc
     2521 aacttaaatt aatgaaaaaa tgttattaat cgttgagcta aagtcattag agatgctttg
     2581 tccttaatgt aaccatatcg caataagtta tgtttttaaa ttgagggcat tattatgaaa
     2641 aaaataccct gcgtgatgat gcgaggtgga acctcgaggg ccgcgttcct gttagcggaa
     2701 catttacccg aagatcaaac gcagcgcgat aaaatattga tggcaattat gggttccggt
     2761 aacgatctgg aaattgacgg tattggcggc ggtaatccac tgaccagtaa agtcgccatt
     2821 attagccgtt ccagcgatcc gcgtgctgat gtcgattatc tgtttgctca agttatcgtc
     2881 catgagcaac gtgtcgatac cacgcctaac tgcggcaata tgctgtctgg cgtaggggca
     2941 ttcgccattg aaaatggttt gattgcagcg acttcgccag ttacccgtgt acgtatccgc
     3001 aacgtcaata cgggtacgtt catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag
     3061 tacgagggta gcgccagaat tgacggcgta ccgggtactg ccgcaccggt tgcgctcact
     3121 ttcctgaatg ccgctggaac gaaaaccgga aaagttttcc cgactgataa tcagattgat
     3181 tattttgacg atgtcccggt gacctgtatc gatatggcga tgccagtcgt cattattccg
     3241 gctgaatatc tgggaaaaac aggttatgaa ttaccggcgg aactggatgc cgacaaagca
     3301 ttattagccc gcattgaatc tatccggcta caagcgggta aagcaatggg cttaggtgat
     3361 gtcagtaata tggttatccc taaacctgtg cttatttctc cagcgcagaa aggcggggca
     3421 attaatgtgc gttattttat gccgcattct tgccatcgcg cgctggcgat aaccggtgct
     3481 attgctattt ccagtagttg tgcattggaa ggcaccgtca cccgacaaat cgtcccttct
     3541 gtaggatatg gcaatatcaa tattgaacac cccagtggtg cgctcgacgt tcatttaagt
     3601 aatgaaggtc aggatgccac gacgttacgc gcatctgtta ttcggacaac cagaaaaata
     3661 ttttccggtg aagtttatct tccctgaaaa aattcgttgt caggataagg acaatcaata
     3721 aaggacttct gtatgagtca tacagaaaga acaggatttt aaatgaataa gaaatcgtta
     3781 tggaagctaa ttctgatatt agcgatccca tgtattattg gttttatgcc agctccggca
     3841 ggattaagcg aactggcgtg ggtgcttttt ggtatttacc tggcggccat tgtgggtctg
     3901 gttatcaagc ctttcccgga acctgtcgta ctgttaattg ccgttgctgc ctcaatggtg
     3961 gtggtcggta acttatccga cggtgcgttt aagaccaccg ccgtattaag cggttactct
     4021 tcaggtacta cctggctggt gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc
     4081 ggtttaggta aacttattgc ctatctgctg attggtaaaa tcggtaacac catgctgggt
     4141 ctgggttacg ttacggtatt cctcgatctg gtactggctc cggcaacacc gtctaacacc
     4201 gcgcgtgcgg gcggcattgt gttaccgatc atcaacagcg tggcgatggc tttggggtcc
     4261 gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga tgatgtccat ttacatggtc
     4321 accaaaacca ccagctatat gttctttacc gcaatggcgg ggaacattct ggcgctgaaa
     4381 atgatcaacg acattctgca cctgcaaatt agctggggtg gatgggcgct ggccgccgga
     4441 ttgccgggca tcattatgct gctggtcacc ccgctggtaa tttacaccat gtatccacca
     4501 gaaattaaaa aggtggataa caaaaccatc gctaaagcgg gccttgccga actgggaccg
     4561 atgaaaatcc gcgaaaaaat gctgctcggt gtctttgtgc tggcgctact gggctggatt
     4621 ttcagtaagt ctctgggggt tgatgaatcc actgtggcaa tcgttgttat ggcgaccatg
     4681 ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc
     4741 ttaatctggt acggcggtat tatcggctta agctccttat tatcgaaagt taaatttttc
     4801 gaatggttag ctgaagtctt taaaaataac ctggcatttg atggtcacgg taacgttgct
     4861 ttcttcgtta ttattttcct cagcatcatc gtgcgttatt tcttcgcttc cggtagtgcc
     4921 tatatcgttg ctatgttacc tgtatttgcc atgctggcga acgtctccgg cgcaccgtta
     4981 atgttaaccg cgctggcact gttgttctcc aactcctatg gcggcatggt tactcactat
     5041 ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa atcctggtgg
     5101 ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct cggcgtgtgg
     5161 tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata cttcaagttg
     5221 catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggggatt
     5281 cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt tattatcttt
     5341 ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta tctgaaaaag
     5401 gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc ggcgaaatta
     5461 aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct cataatacgt
     5521 ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac gatattacct
     5581 ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg ctgccgtatg
     5641 tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac ggtgatgacc
     5701 atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct ccgcatattg
     5761 cggtcatcca tcaatatatg cgtgagatga tggctagcgg gggcaaaatg atcctcgggt
     5821 cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag ggtggcggtg
     5881 agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc gtggttgcgg
     5941 tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg ctggctatca
     6001 ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc gttggaccgg
     6061 gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc acggaaacca
     6121 cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg gcgctgcacg
     6181 gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac gatggctgca
     6241 tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac ccgagcaacg
     6301 tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag attgaaattg
     6361 agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa gtggaaaatg
     6421 gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac tacgaaaacg
     6481 tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc ttctcgctgg
     6541 cggtttaccc gtcatcacag ccggtgttta tggatctcgc caaaaaaggt gtggtagcag
     6601 atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc tttggcgcgg
     6661 gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac ttcccgaacc
     6721 gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgttgatg gacgctcgtt
     6781 ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa cttgattgct
     6841 gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt gtttatcagg
     6901 gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt aaagactggc
     6961 cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag atcctcgacg
     7021 aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat cgttcaaatc
     7081 cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc attaagcaga
     7141 ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg gtctatgcgg
     7201 tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt gtgattggcg
     7261 gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac gtcatcaact
     7321 gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg gattacattt
     7381 acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa ggttatgtga
     7441 tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg acggctgaag
     7501 agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccgt cagatgtaaa
     7561 aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat gctggcgcat
     7621 cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta cgccgcatcc
     7681 ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg cccacgccgc
     7741 ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc tgtatttcgt
     7801 cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc accgcatcgg
     7861 cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc accacctgac
     7921 cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca tcaccggaag
     7981 cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc gtagccggtg
     8041 caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg gtgttatcga
     8101 acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag ctgttggtca
     8161 ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg ttggtgacaa
     8221 agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacttgg tcaccgtcag
     8281 tacgcagtgc taccgccgga tggttacctg catctacgct atcgcccagc gtgttttcga
     8341 tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca gaacacagaa
     8401 caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac gctggtttac
     8461 ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg ctcatgcccc
     8521 catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg tacagagtga
     8581 ttccacctgg agcagcaggg acatacaccg ttccctgata ctccccaggc atcacggcaa
     8641 tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc tgaatcgtgg
     8701 tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc gcaggcaggg
     8761 ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa tagtgttgcg
     8821 catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta ccaggcgcag
     8881 tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc gtcacgccaa
     8941 aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc tccgggctat
     9001 gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc attataaaaa
     9061 agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat aaaatcatta
     9121 tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg atgccatggt
     9181 gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct tctcatagag
     9241 tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga aggaaagacc
     9301 tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa gcggttcacg
     9361 acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat caagtgtttc
     9421 cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa tttttacgcc
     9481 tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact tcatttcgca
     9541 taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc tgagccaaca
     9601 tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa gtcttgatct
     9661 ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta tatggccttc
     9721 agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg ctgatgccgc
     9781 cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg tgatgtcttc
     9841 aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt aatttatgag
     9901 tgtcttctgc ttgattcctc tgctggcgag gattttttcg tagcgatcaa gccatgaatg
     9961 taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt tgtgtcctga
    10021 aaataactca atgttggcct gtatagcttc agtga
//
LOCUS       DAJVRL010000120         9951 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVRL010000120 DAJVRL010000000
VERSION     DAJVRL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.120"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OQN02_003352"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OQN02_003352"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1644032.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OQN02_003353"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OQN02_003353"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1644033.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OQN02_003354"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OQN02_003354"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1644034.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OQN02_003355"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OQN02_003355"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1644035.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="OQN02_003356"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="OQN02_003356"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCS1644036.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="OQN02_003357"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="OQN02_003357"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS1644037.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="OQN02_003358"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="OQN02_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="OQN02_003359"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="OQN02_003359"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1644038.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="OQN02_003360"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="OQN02_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1644039.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9951)
                     /locus_tag="OQN02_003361"
     CDS             complement(8974..>9951)
                     /locus_tag="OQN02_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1644040.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2349 a         2677 c         2506 g         2419 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa t
//
LOCUS       DAJVRL010000121         9927 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVRL010000121 DAJVRL010000000
VERSION     DAJVRL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.121"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..338
                     /locus_tag="OQN02_003362"
     CDS             <1..338
                     /locus_tag="OQN02_003362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644041.1"
                     /translation="SSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNA
                     RNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNI
                     DPLCECFKY"
     gene            526..1641
                     /locus_tag="OQN02_003363"
     CDS             526..1641
                     /locus_tag="OQN02_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644042.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2320..2538
                     /locus_tag="OQN02_003364"
     CDS             2320..2538
                     /locus_tag="OQN02_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCS1644043.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2756..2998
                     /locus_tag="OQN02_003365"
     CDS             2756..2998
                     /locus_tag="OQN02_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644044.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="OQN02_003366"
     CDS             complement(3328..4257)
                     /gene="fdhE"
                     /locus_tag="OQN02_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1644045.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="OQN02_003367"
     CDS             complement(4254..4889)
                     /gene="fdoI"
                     /locus_tag="OQN02_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1644046.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="OQN02_003368"
     CDS             complement(4886..5788)
                     /gene="fdoH"
                     /locus_tag="OQN02_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1644047.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="OQN02_003369"
     CDS             complement(5801..8851)
                     /gene="fdnG"
                     /locus_tag="OQN02_003369"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8264..8266),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1644048.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9045..9878
                     /gene="fdhD"
                     /locus_tag="OQN02_003370"
     CDS             9045..9878
                     /gene="fdhD"
                     /locus_tag="OQN02_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1644049.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2396 c         2543 g         2498 t
ORIGIN      
        1 tgtcgagtaa taatgaatat gacctgttta aaaatagaga aacctcactg tggaaacgca
       61 aagtaccaga aagcgcagcg ctggcaatga cgggaacagt atataaatta tttggtaatc
      121 gcttactcaa cgcccgcaat cagttagata aaaatctcct cgaaatggct atgcaaatgg
      181 tttatggcaa agatatggtg acaaagtccg gggatatatt gattgatata caattaaata
      241 aggatggcag tctccagagt aagcactaca cacttgatgt aggtgaagtt ataaacagtt
      301 ataatattga ccctctttgt gaatgcttca agtattaatc aaacttactt acatgataaa
      361 aatcctggtg acgttattag cctgtatggc actttagata acattcccct tgtagttaag
      421 gaatgcacgg gtaaaaatca tcaaattaat gttaaagcca aattgccaca agaacgaagt
      481 gaaaaagttg aggatatgtg tgaaagaatg aaaggtggca tctctatgtt taaccacacc
      541 acaaaaacag ctgataatat agagcataat ttgcagctaa gttttctcgt gggttcacgt
      601 ccgtcgatca ccaataaata ctcagcaaaa gatgtaggtc cagatatact tgcattgccc
      661 gtcaacgtat atcatgcgga ttttaaaatt aaaatcaata atgcagtgga gaaggcttca
      721 tcaattgtgt tatgtgctgt gcaaaaaaat ctccatgcag caaggaaggt tcttagcggg
      781 ataataggaa atgtgccaaa aaataataaa ccaaaaacaa ttatttttga tttgcgtttt
      841 cttagtgcaa ttaagcagaa agtttttttg ggcagtgaaa aaccgttgtt tgtaaagcat
      901 accgcaatga tgagggaggc atgtaaggaa ttaccgcagt cagtggaata tgttccttta
      961 gcctgtgaag cgcatagcca tcgcagcgtc gcccttgcca aaaccgtggc tgcgcccatc
     1021 atggcgacag gtgtgggctt ggcattaatg tatgcaacat catcaggacc gtattatgcg
     1081 caaagtcttt ctggttcgcc ggatatcgag atgataaaaa atcgcatggc tgaattgttt
     1141 aaccagtcgg atatttctgt gcgcaacaaa tatagcccgg cttttgataa atggaatgaa
     1201 ctggtagata aattaaatca cgaaagaaat acagttccgt ttgcgtgttt gaccaccatt
     1261 ttagcgacgg cgataaatga aactcttgag ggggatacga atgttgccgt tgtgatgggg
     1321 tgtaaatctg cgaaagatcg cacgatatcc attgtccttg gcaactcgat gttgcaaaca
     1381 ctccttgaaa aacgcctcgc cgatagtgga gaaatagaaa atctttttga tcaacaggga
     1441 tattttagtt gcgattcatt aacacccgaa gaattaatga tgttaaaaga cctttttgat
     1501 attcgagttc ttcatgtctc taacaaattc aatgttggat tgcaaggtaa tatcaacact
     1561 gatgttttac aagattcgtt ttttaaaaat gtcgatttca ttcatgaata taactcgtat
     1621 accgtaggga tgggagtctg acgagtaaaa ctcattagga ttatcagaat tcggacaatg
     1681 cattgcaaaa atgacataca aagagagatg tgtgcaggta cttagccgcg aggggcatgg
     1741 cgaagccata cagaaagtac cagtattcag aactccagtg aaagcggcat agtggccgcc
     1801 tgctcaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct atgatgcgat
     1861 taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga tgccgggggt
     1921 gttccggaag gaattccttg tttcccgaac tgttaccaac aaaattataa cgatgagcaa
     1981 gtaaagaccg gttggtggca gtactgaccg gtctgtgatg aaagattaat ttcaccttaa
     2041 atcctggtgc tgttaggatg ctgacgtttt tcggctacac cattcagatt atgcaaataa
     2101 atatccctga atataatata ttcatagcac ccataactca tcctgggcga taactaaacc
     2161 atttttgagt aataaatgaa acaactgcaa cgctcttatt atttctggaa aacgcctcgc
     2221 aaaaacaaaa tgacatgcaa aaatccgcat ttttttaaat gcttttccgg cgtaattttc
     2281 gcacatacat taatgagtaa tatatgtaag gtggtggtaa tggcgatgaa tacggttttt
     2341 cttcatttat cagaagaggc aattaaacgg ctgaacaagc ttcggggctg gcgtaaggtt
     2401 tcacgatctg caattttacg cgaagcggtt gagcaatatc tggagcggca gcaatttccg
     2461 gtgcgtaaag caaagggagg caggcaaaag ggggaggtag ttggtgttga tgatcagtgt
     2521 aaggagcata aggaatgaaa aaaatttttc attcttgaat ataaaaaaca gatgccctta
     2581 ttctggtatt aatacaaggc tgttttactt gaacttataa taactgcaac tgttacatcg
     2641 tatctggaaa acgcctcgca aaaataaaaa atgatgcgta aatgagcttt ctttaaagat
     2701 tcggcaaggg tacttttctc tccatagatt caaaaatgat attggcgaga tatttatgaa
     2761 ctctctggca ggtatagata tgggcagaat tttactcgat ttatcgaatg aggtgattaa
     2821 gcaacttgat gatctcgagg tgcagcgtaa tcttcctcgt gcggacctat taagggaagc
     2881 ggtagatcaa tacctgataa atcaatcgca aacagcaaga accagtgttc ctggcatctg
     2941 gcaagggtgt gaggaagatg gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg
     3001 ggaaaaaatg atgtcaatca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
     3061 gtgaacgcct tatccggcct acaaagtcat gcaaattcaa tatattgaag tttattggta
     3121 ggcctgataa gcgcagcgca tcaggcaatc atacgcttgt catcagtgac atacccataa
     3181 ccttatcgat gtgttggagg gaagaacgag gcaaacagac aattgcggca tgatcttgaa
     3241 tgggtaacgc ggaatacgag agatggtgtc gtcacaccct atgagctgta atccgggcga
     3301 cctgcgccac ccggaaaacg tcagagatta ctctccttca cccggaaaca ggaacgggtt
     3361 aatggaacta cgggcgtagc cttcttgctc cattcgcgcg tccagtacca gagaggcgag
     3421 gtcatctgcc acggcttcaa ctttcggttc tttttcctga taaagaatct tcaggtaagt
     3481 gccgcagtca tcgcagcttt cggctttaat cgcggcctgt tcgtcatcca gcgaccagta
     3541 atgcagtttg ccgctctgct cgcagttgct gcattttacg cgcactacgt gccattcggt
     3601 ttcacacagg ttgcagtgca ggtaacgcag accctgagtg gtgccaattt gcaccatgct
     3661 ggacaccggc atagagccac ataccgggca atattgacgt tgttcgccgt attcagcgcg
     3721 ggctttgccg gggatcagat tggccatctg cgcccagtag agcgacagcg cagcccagat
     3781 aaacggcgct ttatcgctgc tgacggacga gaaatcagag gcaaacagcg cgctggccat
     3841 atcttccagc tcctgagtcg atgccttctc cagattctca atcactgcca gcgccgggcc
     3901 gctcatttca ggtttcagct cagcaattag cgccatcagc agcttttgcc agtgcttatc
     3961 acgcggcaga acgtgaatat ccagcggggg cttgccctgt gcgctggctt ctttaatgcg
     4021 cgcggtcaga tccatctcca gcggatggtc gtacagcacc actttctggg cgtgggcgat
     4081 aagcgcagca aagcgcaggt aatcacccag cggattattt tctgccagct cgcgcagacg
     4141 ctcggcgcgg cggttgtata aattcttgag ccgagggaac aataacggcg gaatcatatc
     4201 cgccgtacgt ttctcgctcg aacccagctc atcttgcggg attatgcgaa tactcattca
     4261 gctttctttt ccgttgtctt gcggacctca cggtaccagc gcgggtgatg tttcttcgcc
     4321 caggcgctgg taacccatcc ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg
     4381 gcgtagatat gcaccatgat aaccacaatt aacgccactg cggcaaatga atgcagcatt
     4441 aacgcgaatc ggatcaccgg gattgagaaa gcaggcgcaa aataaggacg ccagataatc
     4501 acgccgctca ccagcagcag aaccaggaaa ataatcgccg cccagaaaac gcatttctga
     4561 ccgaagttat aacgcccggt gtcacctact tcctcgttga cgacgatctt acgaatattc
     4621 ttcgcccaaa agatatcatc ccgattgatt aggttttgat gccagtaacg gaaaaacatg
     4681 atgatgaacg aggcaaacat aaccacgccg acaaacgggt gcagaattcg cgccagctgc
     4741 ggtgtgccca tgatttgcat caaccagttg aaggacggga acaaaaagcc cagcccgctc
     4801 accgccgcca ggatgaagca gaaggcggtg atccagtggt tgatacgttc cggcgcggtg
     4861 tagcgcacga tggtgtcacg tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat
     4921 tattctcttc ctcatccgca cggttcggac cgacacccac gtagtggaag atactggccg
     4981 caaaggtagc cgcaaagcca acagccgcga gcggtttcca gatgcctttc cagaatttca
     5041 cggtttcgct gatttccggg ttctccggca agccatgata cagatttggc ttgtcagcat
     5101 ggtgcagcac gtacatgacg tgtgtaccac cgacgcctgc cggatcgtac agacccgcat
     5161 tgtcgtaacc gcgggttttc agctcagcaa cgcgctcgct cgccagcgtt ttcatcgact
     5221 ctttcgtgcc gaagtgaatc gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc
     5281 caaccaccac gcggtcaacg cacagcgtac atttgtagac gcggttgtct tccgggttga
     5341 ggcgcggaat gtcgaacgga cagcccgcaa tgcaataacc gcagccaatg cactgctcgg
     5401 actggaagtc gacgataccg ttggcatact gaataattgc accttccgcc gggcacgctt
     5461 tcaggcagcc tggatcggaa cagtgcatac agccgtcttt gcggatcagc cattccagtt
     5521 tgtcgttctg ctccacttcc gagaagcgca ttaccgtcca cgatttggcg cttaaatcat
     5581 tggggttgtc gtacacccca atgttattgc cgacggtatc gcgaatgtcg ttccactctg
     5641 aacaagccac ctgacaggct ttacagccga tacaggtggt aacgtcgatg agtttcgcca
     5701 cttcttcctg gaagtcccgc gcctgaggcg cgggggtcag accgttagtc gcggaacgac
     5761 gaatgatatc ttgagattga taagccatat gtcgtctccg ttacaccttt tccacattca
     5821 caaggaagga cttaaactcc ggcgtctgcg tgttcgcatc accaacgaat ggcgttaaag
     5881 tattggcaat aaagcctttt ttagctacgc cttcatagcc ccagtgaatc ggaataccga
     5941 tggtatcgat atctttgccg ttcgctttca gcgtgcgaat acgtttggtc accaccgctt
     6001 tggctttgat atagccacgg ttggaggaga ctttcacggt atcgccctgg gcaatgccaa
     6061 gtttattcgc cagcgactcc ccgatttcca caaactgctc tggctgcaaa atcgcgttca
     6121 acagcgcgtg tttggtccag tagtggaagt gctcggtcag acgataggtg gttccgacat
     6181 acgggaactt atcggcttta cccaatgctt cggcgtcgtc tttaaagata cgtgcagctg
     6241 ggttcgagat aacgtttgga tgcagcgggt tagttcccag cggcgtttca aacggctcgt
     6301 agtgttccgg gaacggacct tccgccatct tatcgagggc aaacagacgc cccatgcctt
     6361 cctgctgcat gataaacggc ccgacgccgc tacccggagg cgctgcgctg tagtccggaa
     6421 tatcccagcc ggtccactta gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg
     6481 ggttaccctg cggatctgcg gaggcgcggt tatacagaat gcggcggtta agcggccatg
     6541 cccatgccca gcccagcgtg ttaccgaggc cagacggatc ggcgttatca cgacgcgcca
     6601 tctggttgcc ttccggcgtc cagctaccgg cgaaaatcca gcagccacag gaggttgtac
     6661 cgtcatcgcg caattgggcg aacgagctaa gttgttggcc tttcttgacg ataaccgcac
     6721 cggttgccgg atcggtaata tcggccagcg ccttaccgtt gctctccatc gctacttctt
     6781 ccgacttcgg ctcatgcgga atggcgtagt tccaggtcat gttcagcacc tgatccgggt
     6841 tcgcgccacc ctgttcggca tacatcttgc gcaagcgcag gaagataccg gagaggatct
     6901 caccgtcagt cagcgcaatc cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc
     6961 gacctgagtt aacgattgaa ccgttctctt ctgcgaagca ggtcgatggc agacggaaca
     7021 cttcggtctg gatcttcgac gagtcaactt cgttcagctc accgtggttc tgccagaagt
     7081 tagaggtttc agtgttcagt gggtcgatgg tgacgaggaa cttcagtttc gacagacaac
     7141 cgatcacttt gtttttgttc gggaatgagg caacagggtt aaagccctgg cagatatagc
     7201 cattgacctt gccctctttc atcatctcga agtattgcag gacgtcgtag cctttatccc
     7261 acttcggcaa ccagtcaaag ccccagctat tttccgccgt cgctttatca ccaaagaagg
     7321 ccttcatcat agagacgaag aatttcgggt agttgcccca gtagtttacc tggccttcca
     7381 gcagcggttt tggcgtgttg gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg
     7441 gcagcgtcat gtaacctggc agactctgcg acagtagccc caggtccgtc agcccctgaa
     7501 tattggagtg accgcgcagg gcgttaacgc cgccgccggc catccccata ttgccgagca
     7561 gcagctggat catcgccatc gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc
     7621 cgagggcata caggaacgag gcagttttat cgtgagcact ggtttctgcg atgtattcgc
     7681 agactttcag gaacgcgtct tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt
     7741 agcgggaaac gtgctgtttc agcaagttcc acacgcagcg cggatgttgc agcgtggtat
     7801 cgcgtttggc gaagccgttt tcgtccagtt cataagtcca ggaggattta tcgtacttgc
     7861 gtttttccgc gtcgtagccg gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga
     7921 tcaggctggc gttggtatag gcttcggtgt attcgtggtt gaatttttca ttgttcagca
     7981 ggtacagcaa tacgcctgac aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt
     8041 agtcagctac cgccgccgta cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccgt
     8101 tgtgaatttt ggcttccatc gcccagcgga acccgaccgg gtgagcttca gcggcgttac
     8161 cgcccatcac cacgacgagg ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac
     8221 cgcgaccaaa tgttggagca agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt
     8281 tgtcgaccgc gagcataccc agcgcgcggg agaatttttg cgttaaatag ccggtttcgt
     8341 tactcgacgc ggaagcacac agcatcccgg tggagagcca gcggttaaca gtcacgcctt
     8401 cggcgttttg cgcaatgtag ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat
     8461 caaacgcctc ttcccaactg atttgctgcc atttatcaga acctggcgca cggtattccg
     8521 gaaacttcag acggctttcg gagtggatga aatccaccag gccagcgcct ttcggacaaa
     8581 gtgcaccgcg gttgaccggg tgatccggat cgccttcgat atggaagata gatgctttgg
     8641 cgttttttgc tccgtcaccg aggctgtaca tcaacagccc acagcctacg gaacaatagg
     8701 tgcaggtatt acgggtttcg cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg
     8761 ctacgctggg tgcaaaaccc agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga
     8821 tcttaaagaa ctgccttctg ctgacctgca tggattgctc cttgtttcga cattgtcacg
     8881 tcccatttac attcgcttgc tgcgtgtgca gggagtggga gttatttttc tttgcggaag
     8941 gggccgcaaa ggtccagaat tggctcaatt tccctccatc taggaagggt ttgtaacaga
     9001 ataccataat gttggtgtgt atgttcttat ctggttaaga gaaagtgaaa aaaacacagc
     9061 gaaaagaaat cgaaaatgtg acaaatatca caggtgttcg tcaaattgag ttatggcggc
     9121 gtgatgattt acaacaccca cggttagatg aggtcgcgga agaagttccc gttgcgctgg
     9181 tctacaacgg catttcgcat gtggtgatga tggcgtcgcc caaagacctt gagtactttg
     9241 cgctcggttt ttcgctttcc gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg
     9301 atgtcgttcc ttcctgtaat ggtcttgaag tacaaattga gctttccagc cgccgcttta
     9361 tggggttgaa ggagcgccgc cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg
     9421 tggagcaact taatgacatc ggaaaaccgg tgcagccgct accgttcacc cagacgtttg
     9481 atctcaacaa actggatgat gcattacgtc atctcaacga ttttcagcca gtggggcgac
     9541 tgactggttg tactcacgcc gctgcctgga tgttgccatc tggcgaactg gtcggcgggc
     9601 atgaagacgt gggtcgccat gtggcgctgg acaaactgtt aggccgccgg tcacaagaag
     9661 gggaaagctg gcagcaaggt gcggtactgg tttccagtcg tgccagttat gaaatggtgc
     9721 aaaagtcggc gatgtgcggc gtagagattt tgtttgcggt gtctgccgcg accacgcttg
     9781 ctgtagaagt ggctgagcgc tgtaatctga cactggtagg tttttgtaaa ccgggtcggg
     9841 caacggttta tacccatccg cagcgtttga gcaattaatt aaaataagtc ctgcgatata
     9901 tattaaattg caggacttat tcatttc
//
LOCUS       DAJVRL010000122         9906 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVRL010000122 DAJVRL010000000
VERSION     DAJVRL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.122"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(161..901)
                     /gene="pflA"
                     /locus_tag="OQN02_003371"
     CDS             complement(161..901)
                     /gene="pflA"
                     /locus_tag="OQN02_003371"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1644050.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1093..3375)
                     /gene="pflB"
                     /locus_tag="OQN02_003372"
     CDS             complement(1093..3375)
                     /gene="pflB"
                     /locus_tag="OQN02_003372"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1644051.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3430..4287)
                     /gene="focA"
                     /locus_tag="OQN02_003373"
     CDS             complement(3430..4287)
                     /gene="focA"
                     /locus_tag="OQN02_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1644052.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4693..6453)
                     /locus_tag="OQN02_003374"
     CDS             complement(4693..6453)
                     /locus_tag="OQN02_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCS1644053.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6583..7209
                     /locus_tag="OQN02_003375"
                     /pseudo
     CDS             6583..7209
                     /locus_tag="OQN02_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7408..8496
                     /gene="serC"
                     /locus_tag="OQN02_003376"
     CDS             7408..8496
                     /gene="serC"
                     /locus_tag="OQN02_003376"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1644054.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8567..9850
                     /gene="aroA"
                     /locus_tag="OQN02_003377"
     CDS             8567..9850
                     /gene="aroA"
                     /locus_tag="OQN02_003377"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1644055.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2368 c         2482 g         2535 t
ORIGIN      
        1 attcgatggg gaagaacttc acgcgagcta ttatcagtcg ttattatctc aggtacggga
       61 tatggtagat gcactataag atgtgttaaa aacgctgtag cagaatgaag cgcggaataa
      121 aaaagcggca actcaataaa gttgccgctt tacgaggaaa ttagaacatt accttatgac
      181 cgtactgctc aagaatgcct ttcacgcgtt ccatggtctc tttcttcggt ggtttaacac
      241 catcgagttt gtattcttca cccatcgcca cccatttgtg tttgcccagt tcgtggtagg
      301 ggagaagttc gattttctca acgttaccca tatcacgggt aaattcaccg aggcgatgtg
      361 ccgaatcgtc atcgtcagac cagcctggga caacaacgta gcggatccac accttcacat
      421 ttttgttcgc cagatattta gcgaactcca gcgtgcggtg gttggaaact ccaaccagat
      481 tttggtggat ctcgtcgttc atctgtttga gatcgagcat taccaggtcg gttacttcca
      541 gcagttcatc aatcaccgga tcgtaacgac gaacaaaacc gttggtatcc agacaggtat
      601 gaatgccttc ttttttacag gcgcggaacc agtcacgaac aaactcagct tgcaggattg
      661 cttcaccgcc ggatgcggta acgccgccgc cggaagcgtt cataaagtgg cgataggtca
      721 ccacttcctt catcaaatct tcaacggtaa cttctttacc gccatgcgtg tcccaggtgt
      781 cgcggttatg acaatacagg cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa
      841 tacctgggcc gtctacggtt ccacaggatt caaaggagtg aatgcgacca ataactgaca
      901 ttgcggtgtt tctccagatg tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct
      961 acgtcgagtc tgttttggca gtcaccttaa agtatagata gctgacaaaa aaggctctcg
     1021 tgctaaaaaa ggccccactt tcgtggagcc tttattgtac gctttttact gtacgatttc
     1081 agtcaaatct aattacatag attgagtgaa ggtacgagta ataacgtcct gctgctgttc
     1141 tttagtcagc gagttgaaac gtactgcgta gccagataca cggatggtca gctgcggata
     1201 tttttccggg ttttccatcg cgtcgagcag catttcacgg ttcatcacgt taacgttcag
     1261 gtgctgacca ccttcgatgg atgcttcgtg gtggaagtaa ccatccatca gaccagccag
     1321 gttggtctta cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag agaaggtgta
     1381 ggagatacca tctttagcgt aagcaaacgg cagtttagca acggaagtca gagacgctac
     1441 agcacctttc tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc
     1501 acgacgaccg tctggggtgt taccagtttt cttaccatac acaacgttag aagtgatggt
     1561 cagaacagac tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat
     1621 gaaacgttct accaggtcaa cagccaggtc atctacacgc ggatcattgt taccaaactg
     1681 cgggtattcg ccttcgattt cgaagtcgat agccagaccg tcttcgtcac gaatcggttt
     1741 aactttcgca tatttgattg cagacaggga gtcagcagca acggacagac cagcgatacc
     1801 acacgccatg gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct
     1861 gtacttgtcg tgcatgtagt ggatgatgtt cagtgcagtg atgtactgtt tagccagcca
     1921 gtccatgaag tgatccatgc gctccatcac ttcatcgtag ctcaggagat cgcctttgat
     1981 cggttcagac ttcggaccaa cctgcatttt cagtttttcg tcaacgccgc cgttgattgc
     2041 gtacagcatg gttttcgcca ggtttgcacg cgcaccgaag aactgcattt gtttaccaac
     2101 gatcatcggg cttacgcagc aagcgatagc gtagtcatcg ttgttgaagt ccggacgcat
     2161 caggtcatcg ttctcatact gcagagaaga ggtgtcgatg gacactttag cggcgaattt
     2221 cttgaagttc agcggcagtt tttcagacca cagaatggtc atgttcggtt ccggagacgg
     2281 ccccatagtg tacagggtgt tcaggaaacg gaagctgttt ttggtaacca gggtacgacc
     2341 atcgaggccc ataccaccga tagattcggt tgcccagatt gggtcgccag agaacagttc
     2401 atcgtattcc ggagtacgca ggaagcgaac catacgcagt ttcatgacca ggtggtcaac
     2461 catttcctgc gcttcttgtt cggtgatctt gccagctttc aggtcacgtt cgatgtacac
     2521 atccaggaag gtggaggtac gaccgaagga cattgcagca ccgttctgag acttaacagc
     2581 agccaggtag ccgaagtaag tccactggat agcttcctga gcgttggtag ccggaccaga
     2641 gatgtcgtag ccgtatttcg cagccatttc tttcatctga cccagagcgc ggtgctgttc
     2701 agcgatttct tcgcgcagac ggatagtctg ttccaggttt acgccgtttt ccagatcagc
     2761 ctgtagagag gtgaactgag cgtatttgtc tttcatcagg tagtcgatac cgtacagcgc
     2821 aacgcgacgg tagtcaccga tgatacggcc acggccataa gcatctggca gaccggtcag
     2881 aacaccggat ttacggcaac gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt
     2941 gtgagtttta cggtattcag tgaagatttt tttgatcatc gggtccagtt cgcggttgta
     3001 cgctttgcag gaaccttcga tcattttgat accaccgaac gggataagag cacgtttcag
     3061 cggagcttca gtctgcagac caacaacttt ttccaacgct ttattgatgt agccagcgtc
     3121 gtgagaggtg atggtggaag caacagcggt gtcaaaatca actggcgcgt gagtgcggtt
     3181 ttccagttta acaccttcca ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc
     3241 agccaggaag gactcgtcac cctcgtacgg cgtgtagttt ttctgaatga agtcacggac
     3301 gtttacttca ttctgccagt cacctttggt aaaaccttcc caggctgtgg ctaacttttc
     3361 attaagctcg gacatgtaac acctaccttc ttaagtggat tttttattta ctgcgtactt
     3421 cgacaaccat taatggtgat cgttttcacg caggtaaatg acccagtatg tcaacccaac
     3481 caacaaacca ccgccgataa tattaccgat cgtaaccggg atcaggttat cagtgatgaa
     3541 gttcatcacg gtcaggtgag aaaaattttc cggtgcagaa ccgacagcgg tccagaattc
     3601 cggagatgcg aagtcgcgga ttacaatacc catcgggatc ataaacatgt ttgcgatact
     3661 gtgctcaaaa ccgctggcaa caaacatcgc gaccggcagc accataatga acgctttgtc
     3721 catcaggctg cggccagaat aactcatcca tactgccaga cataccatca ggtttgccag
     3781 gataccaaga cagacggcct caataaaagt atggtgcact ttgtggtcgg cggtttgtag
     3841 gacgtttagt ccccattgac catttgcggt catatactcg ccggaaagcc acattaaaag
     3901 tacaaacagc agtgcgccga ccaggttgcc aaaatagaca tttagccagt ttttagccaa
     3961 ctgacaccag gtgatgcgcc cactcgcctt agcaacaaca atcaacactg tggaagtaaa
     4021 gagatcggct ccgcagacaa cacaaagaat cagccccaga gagaagcaaa tgccgccaac
     4081 cagttttgcc atgccgaagg gcattgtgcc tgtgccagtg gttgctgtga tatagaagac
     4141 gaatgcgatt gagatgaaaa caccggcggt aatcgccaga tagaaagtct taagcggatg
     4201 tttcgttgct ttatagacac ccgcctcttc ggccactttg gccattgcag caggaagtaa
     4261 aagatcaaaa gggttgtcag ctttcacact aactctctct ttattaagtc ggcgacgaga
     4321 tactaacaaa gcattataga tgagaaattg atatagatca tatctcgcct ggcttatagg
     4381 cccgtaactc gcatggtttt tatgcaaata cggagtaact atttgattat ccaaataaaa
     4441 ataaatttta aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt
     4501 aattaaaagg gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa
     4561 attaccgata tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg
     4621 gggcattaaa aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta
     4681 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
     4741 cttcaacaac gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc
     4801 gtaaaacgcc gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta
     4861 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa
     4921 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt
     4981 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
     5041 cagcatggct tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc
     5101 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa tcttcttcat ccagttgctc
     5161 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
     5221 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
     5281 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
     5341 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
     5401 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
     5461 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
     5521 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
     5581 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     5641 aaagcaggtg ccgtttgcag gattaaacag taccacgcaa atcaccggat actggccgcc
     5701 aagggaacca tcatatgcga agatcgggaa accttctgtt tccagtgttt cgatcgcttc
     5761 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     5821 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     5881 ttcgttgcgg gtattgccag cggacatacc gttagaaacg tacaggttac caatgatatt
     5941 catcggaata taaacggtct gattgtcgga ctggcgcgta aacggcagac cgcaaatacc
     6001 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     6061 atcgtaaaat gcgcgcagac ggtcatcgag cagcccttct ggcacatcgt cattttcgct
     6121 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     6181 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     6241 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     6301 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     6361 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc
     6421 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     6481 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     6541 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     6601 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     6661 ctttatacct ttgtactggt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac
     6721 agatgtcgct gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg
     6781 cgttttatga tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt
     6841 tataccgcct ggtaatatga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     6901 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     6961 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     7021 gtgaaaccgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     7081 gagtcggcgg actatgcctg cattcgttgt agtgaaatca ttcatatgaa cgcgggggaa
     7141 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     7201 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     7261 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     7321 aaaaccgata gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt
     7381 aatagcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     7441 gcaatgctac cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     7501 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     7561 gaagccgaga aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc
     7621 tgccatggcg gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa
     7681 accaccgcag attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa
     7741 aaatactgca cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt
     7801 aagccaatgc gtgaatggca actctctgat aatgcggctt atatgcatta ctgcccgaat
     7861 gaaaccatcg acggtatcgc catcgacgag acgccagact tcggcaaaga tgtggtggtc
     7921 gccgccgact tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta
     7981 atttacgctg gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt
     8041 gaagatttgc tgggcaaagc gaatatcgcg tgtccgtcga ttctggatta ttccatcctc
     8101 aacgataacg gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg
     8161 gtctttaaat ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa
     8221 aaagcagaac tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg
     8281 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa
     8341 ttgttccttg aagagtcttt tgctgcaggc cttcatgcac tgaaaggaca ccgtgtggtc
     8401 ggcggaatgc gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca
     8461 gacttcatgg ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca
     8521 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac
     8581 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     8641 taaccgcgct ttattgcttg cggcattagc acacggcaaa acagtattaa ccaatctgct
     8701 ggatagcgac gacgtgcgcc atatgctgaa tgcattaaca gcgttagggt taagctatac
     8761 gctttcagcc gatcgtacgc gttgcgaaat tatcggtaac ggcggtccat tacacgcaga
     8821 aggtgccctg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     8881 tctttgtctg gatagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc
     8941 gattggtcat ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca
     9001 agaaaattat ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga
     9061 tggctccgtt tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     9121 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact
     9181 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     9241 aaaaggcggg cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc
     9301 ggcttcttac ttcctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat
     9361 tggacgtaac agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc
     9421 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga
     9481 tatggatatg aaccatattc ctgatgcggc gatgaccatt gccacggcgg cgttatttgc
     9541 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaga ccgatcgcct
     9601 gtttgcgatg gcaacagaac tgcgtaaagt cggcgcggaa gtggaagagg ggcacgatta
     9661 cattcgtatc actcctccgg aaaaactgaa ctttgccgag atcgcgacat acaatgatca
     9721 ccggatggcg atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga
     9781 tcccaaatgc acggccaaaa catttccgga ttatttcgag cagctggcgc ggattagcca
     9841 ggcagcctga atgaacaacg ggcaataaat agccaaatct ttctttatca aaacgtcggc
     9901 acattg
//
LOCUS       DAJVRL010000123         9625 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVRL010000123 DAJVRL010000000
VERSION     DAJVRL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.123"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OQN02_003378"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OQN02_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS1644056.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OQN02_003379"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OQN02_003379"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1644057.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="OQN02_003380"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="OQN02_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OQN02_003381"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OQN02_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCS1644058.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OQN02_003382"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OQN02_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1644059.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="OQN02_003383"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="OQN02_003383"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1644060.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="OQN02_003384"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="OQN02_003384"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1644061.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="OQN02_003385"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="OQN02_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="OQN02_003386"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="OQN02_003386"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1644062.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2489 g         2269 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaat
//
LOCUS       DAJVRL010000124         9596 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVRL010000124 DAJVRL010000000
VERSION     DAJVRL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.124"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OQN02_003387"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OQN02_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1644063.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OQN02_003388"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="OQN02_003388"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1644064.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OQN02_003389"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="OQN02_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1644065.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="OQN02_003390"
     CDS             complement(2404..2607)
                     /locus_tag="OQN02_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644066.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /gene="ddlA"
                     /locus_tag="OQN02_003391"
     CDS             2685..3779
                     /gene="ddlA"
                     /locus_tag="OQN02_003391"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1644067.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="OQN02_003392"
     CDS             complement(3803..4015)
                     /locus_tag="OQN02_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1644068.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="OQN02_003393"
     CDS             4275..4583
                     /locus_tag="OQN02_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1644069.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OQN02_003394"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="OQN02_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1644070.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OQN02_003395"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="OQN02_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1644071.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="OQN02_003396"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="OQN02_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1644072.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="OQN02_003397"
     CDS             complement(8479..8982)
                     /locus_tag="OQN02_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1644073.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9596)
                     /locus_tag="OQN02_003398"
     CDS             complement(9070..>9596)
                     /locus_tag="OQN02_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1644074.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2382 a         2448 c         2283 g         2483 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attaccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttcct
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat tatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accactgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatat gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg cgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     9241 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     9301 agcctgatct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
     9361 gctgtcgtaa ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     9421 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     9481 gtcgtaaccg gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     9541 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaagc
//
LOCUS       DAJVRL010000125         9497 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVRL010000125 DAJVRL010000000
VERSION     DAJVRL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.125"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..1000
                     /locus_tag="OQN02_003399"
     CDS             <1..1000
                     /locus_tag="OQN02_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HCS1644075.1"
                     /translation="IIATGGDYLMAKLKGDGSGKSASHEPESHATSPARERNNLGRDL
                     NPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFDKVSALADSAPTTASSAPKSTSSSN
                     VSPAGSASAPGSATPAGQSQDNGLARITGVKRLGLDPDLYIPADRHIPCAMMQRFVSD
                     VGGRFNCLISEDIYSASNAVRLIPAGTEARGLYRTGTLKNGQGRLFLAITELRTPEPG
                     RLVIPMVDSQAVGALGENGVAGWIDNHWLERIGNTLLLGTVQDFAAAASGSSPGKDRN
                     TDYTENTRAATAEMAKTLLENSINIPPTMYLNQGDVIGLVTGADIDFSDVYRLRMR"
     gene            1004..2053
                     /gene="virB11"
                     /locus_tag="OQN02_003400"
     CDS             1004..2053
                     /gene="virB11"
                     /locus_tag="OQN02_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HCS1644076.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
     gene            2040..2441
                     /locus_tag="OQN02_003401"
     CDS             2040..2441
                     /locus_tag="OQN02_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644077.1"
                     /translation="MMLPKLTGLTAIVVLLAGCSSPPEPAAVDWSQPAQPVTTTRPVW
                     EPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHASRAVVNAPDGQRFFAAKT
                     WLREGGYKGVVEFRPRSGCLTCDTSEIVFYR"
     gene            2532..2849
                     /locus_tag="OQN02_003402"
     CDS             2532..2849
                     /locus_tag="OQN02_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644078.1"
                     /translation="MKHTVITAALLCALTSPAFAADGQNADPNDPCAMVLCLAGKLDG
                     SSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSACPSADSAIVGKIISKFGRM
                     NGW"
     gene            2876..3211
                     /locus_tag="OQN02_003403"
     CDS             2876..3211
                     /locus_tag="OQN02_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644079.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            3187..3495
                     /locus_tag="OQN02_003404"
     CDS             3187..3495
                     /locus_tag="OQN02_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644080.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            3492..5693
                     /locus_tag="OQN02_003405"
     CDS             3492..5693
                     /locus_tag="OQN02_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HCS1644081.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            5690..6082
                     /locus_tag="OQN02_003406"
     CDS             5690..6082
                     /locus_tag="OQN02_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HCS1644082.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
     gene            6791..8698
                     /locus_tag="OQN02_003407"
     CDS             6791..8698
                     /locus_tag="OQN02_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HCS1644083.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
     gene            8713..9459
                     /locus_tag="OQN02_003408"
     CDS             8713..9459
                     /locus_tag="OQN02_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCS1644084.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
BASE COUNT         2267 a         2662 c         2678 g         1890 t
ORIGIN      
        1 cattatcgcc accggcgggg attacctgat ggcgaaactg aagggtgacg gcagcggaaa
       61 aagcgcgagc catgaaccgg aatcgcacgc cacctccccc gcccgcgagc gcaataacct
      121 cggacgggat ctgaacccgc tgggacagct ggcagacaca accggccagg agaagactac
      181 tccgactgat accgccgcgc cgccgcaggc atcaccgccc cccctgacgt ttgacaaggt
      241 cagcgccctg gcagacagcg ccccaaccac tgcaagcagc gccccgaaaa gcacgtccag
      301 cagtaacgta tcacctgcgg gcagcgcgtc cgcgccaggc tctgcaacac ccgccgggca
      361 gtcgcaggat aacgggctgg cgcgcattac cggcgtgaag cggctcggcc tcgatccgga
      421 tttgtacatt ccggccgacc ggcatatccc ctgcgcgatg atgcagcgct ttgtatcgga
      481 cgtgggcggg cgctttaact gccttatcag cgaggacatt tacagcgcca gcaacgccgt
      541 gcgccttatc ccggcaggca ctgaggcgcg cggcctgtac cgcaccggca cgctgaaaaa
      601 cgggcagggg cgtctgttcc tcgccatcac cgagctgcgc acgccggaac ccggccgcct
      661 ggttattccg atggttgaca gccaggccgt gggggcgctt ggcgagaacg gcgtcgcggg
      721 ctggatagac aatcactggc tggagcggat aggtaatacc ctcctgctcg gcacggtaca
      781 ggactttgcc gctgcggcct ccggctcgtc gccgggcaag gatcgcaaca cggactacac
      841 ggagaacacc cgcgcggcca cggcggaaat ggcaaaaacg ctcctggaaa acagcatcaa
      901 catcccgccg acgatgtacc tcaaccaggg tgacgtgatt ggcctggtca cgggggcgga
      961 cattgatttt tcagacgtct acagactgcg catgaggtaa gcgatggacg ataaactgac
     1021 tgataccccg tcagagggca acgtcgcgct gtcacacctg cgcgatctgt tgttcgggga
     1081 ctacctgagc cgggaggggc tgacggaaat tgccatcaac cgccccggag agctgcacac
     1141 aaaaatcaac ggggcgtggg aaatgcacgc ctcgccggtc acgctgcgcc agtgctcgtc
     1201 ctttgccagg gcgctggccg tgtggaacaa cgacaccatt gatgaaacgt cgcctatcct
     1261 ctcggccacg ctccctacag gagaacgctg ccagatagtg gtgcccccgg cctgtgagcg
     1321 taacaccatc tcaatcaccc tgcgtaagcc ggactacttc cagcgcaccc atcagtcgta
     1381 tatcgatgcc gggttttatg accgggtgca gggtacagaa aaggtggaat caaaagacgg
     1441 ggagctgagc cgcatatacc tgtccgggaa tatcccgctt tttatggaaa agtgcgtgga
     1501 gtacggcaaa acgctctttg tggtggggga aacaggctct gggaaaacca cctacatgaa
     1561 gatgctgctg cactacattc ccgagacgct gcgccttatc accatcgagg acaacccgga
     1621 gctgaagttt tacagccaga aaaactacgt tcatctgttc tacccggcgg acgccggaga
     1681 gggcgcaata gtcacctctg atcgcctggt gcgcgcgatt taccgtatga accccgaccg
     1741 ccccctgctc gccgaggtgc gtggccgcga ggcgtgggac gcactcaagc tgattgagtc
     1801 cgggcacgag gggtttatgt cctccctgca cgcgggcagc ccgcgcgagt gtatctccgg
     1861 gctgattgac cgctgctacg agcatccggc gtgccggggc atgccggttg acgtgctgct
     1921 gcgcaaggta ctgcactgcg tcgacgtcat tgtgagcgtg gacgttcacg gcaacgtgcg
     1981 ccgcatgagc gacgtctatt ttaaacccct gcacatgcag gctatgaagg agagtttcaa
     2041 tgatgctgcc taaactgacc ggcctgacgg cgatcgtcgt cctcctggcg ggctgttctt
     2101 cgccaccgga gcctgcggcc gttgactggt cacaaccggc gcagccggta acgaccaccc
     2161 ggccggtatg ggaacccaac cgtcttatcg tggcctcgcc ggtgacggaa gggcactggt
     2221 cgcaggtgct tacgcgtttt gaacccggcg ggatttaccc ggccgcacag tggtacgcgg
     2281 ttgcgcacgc ctcgcgcgcg gtagtaaatg ccccggacgg ccagcgcttc tttgcggcca
     2341 aaacctggct ccgcgagggc ggctacaagg gtgtggtgga gtttcgcccc cgctccggct
     2401 gcctgacctg cgacacctca gaaatcgtct tctaccgata acgtcggtga cggtctgagt
     2461 ccttcagacc gtcaccgacg ttgaacaacc aacgggcgtt agcgccctgc ttcccttcag
     2521 aggaaatcat catgaaacac accgtaatca cggcggcgct gctgtgcgcc cttacttctc
     2581 cggcgttcgc cgccgatggc cagaatgccg acccgaatga cccgtgcgcg atggtgctgt
     2641 gcctggccgg aaagctcgac ggcagcagtc ccgccgagtg tgacccgatg tacaaaaatt
     2701 tcatgagcat caaaaagaaa aaaaaggggt cgtttttacc cgatcacacc gccgcagcgc
     2761 ggcagaaaaa actgagcgcc tgcccgtccg ctgacagcgc cattgtcggc aagatcatct
     2821 cgaaatttgg ccgtatgaac ggttggtaaa acggcagccg taaaggagac accacgtgat
     2881 taatcaaacc gtttctatta ataacttttc cgggctgctg aaagatgagg agcttcctgc
     2941 aggtgatttg cagacggtgc tgctcgtttt gtcggaaatc agccagaaac aggatcattt
     3001 tcaggccaac acgtccgaac tcgtccagct cgccgcgcag cactcgccga ccttccaggc
     3061 attaccagca gaacgtcagt cgttcttccg ctccgtgctc agtatgccgg tcttctttta
     3121 caccgttccg ctgagcgacg acgaggcggc ggaatgggaa aaactcaacg ataaggatgc
     3181 actccaatgg caacaaaaac cggatttata gccccgccgt ggccgcatgt gcgtggtgat
     3241 tccgttagtg ccgcgagcct gatttctcgc atcgagaaag aagcgcatcg cggctgcggc
     3301 ctgcattacg aaatctacca atatcgcgcc ctgtgcgaat tgcacgatgt tctgaccagt
     3361 ctcagcgtcc gtgatgctga gacgttcatg acagtagcgg cggatcatgg ctttagtctg
     3421 tctgataacg actttcagga aagtcgaatg atgtacttcg aaacgatgga tgaaatcagg
     3481 agagatcaag aatgagactg tggattgcag aaaaacccgc agtcgcgagc gatatcgcga
     3541 aagcgctggg cggcaacttc acccggcacg acggctatgc cgaatcggcg accgatatcg
     3601 tgagctactg cgtcggccac gtgctggaaa tggtgccccc ggagacgatc aacccggcct
     3661 acgccacctg gtcgctcgac accctgccgc tgaagctgta cccggtacag cttcagccaa
     3721 aagcgagcgt ggcaaagcag gctaataccc tggtgaagct gattcggcgc ccggatatca
     3781 aaatggttgt ccactgcggc gacccggacg acgaggggca gctcctggtt gacgaggtgc
     3841 tggagttcgc cggaaacacc gcgccggtga aacgggcgct tattaacgac aacaccgcgc
     3901 cagcggtgaa aaaagccatc aactccctgc gcgataacag cgattttcgt gggatgtacc
     3961 tgaaagccct gatgcgcagc gcaggagacg cgatttttgg cttcagtatg agccgctgtt
     4021 acaccctcaa agcccgcgat aagggctata agggcacgat ttcggttggc cgcgtccaga
     4081 cgccggtgct gggaatgatt gtgcgccggt ggcgcgacaa tcaggcgcac agcgaggcgt
     4141 tttactacca gctcgcaggg cagtttatca gcggtacgga cgttgtctgc gcccgctggc
     4201 agacgtccga atacgcgccg gtggacgata aaaagcggct gacggataag gcctgggggg
     4261 aaggactcgc gagggctctg gccggaaaac cggccagcgt gctcgcggcc gccaccgacc
     4321 ggggaaaaac cacggccgca ccgctgccgt ttaacctgct gcgcctccag gtgtatatga
     4381 accgcaggca caaactgacc gcgcagcaga cgctcgatat cacgcagaag ctcaaggaca
     4441 gtaaatacat tacctacaac cgctctgact gttcttatct gtccgatgaa caattcaacg
     4501 acgcgccaca ggtggtggcg gccttaaaaa cgctcgacgt ttttaacggc cttaccaccg
     4561 acacggcgca aaaaagtgcg gcgtttgaca gtaaaaaagt caccgcccac accgccatta
     4621 tcccgacgac gaacatgccg gatttaagcc gccttaccga caaggaaaag gcggtttatc
     4681 tcaccatcgc gcagttttat ctcgcgcagt tcgtggcgaa aaagcgctat gacgagtcga
     4741 tcgcggagat taaatgcggt gacgagatgt ttaaagtctc ggcgcgtaaa atcaccgatg
     4801 cgggttttac gacgttcctg aacgacgcgg aagaagacga cgaggaggac gaaaacagca
     4861 cctcgtttga ggccatcagc cgcctgcaaa ccggggcgac attaacctgc cgcgaggtgg
     4921 tgatttcgga gaaaaaaact aaaccgctgc cgctgtttac cgaggcgacc ctgctcgctg
     4981 ccctcgttcg cgtggccgat tttgtcgccg atccgcgcat taaaaaattg ctcaaggaga
     5041 aggataaaga caaaaaagat gaacacggcg gcatcggcac accggcgacc cgcgcggcta
     5101 ttattgagac gctgaaaacc cgcgattaca tcaccgtgga aaagggcaaa tttatcccaa
     5161 ctgccgccgg gctggcgtta attgatgccc tgcccgactc ggtgacgcag ccggatatga
     5221 ccgccgtatg ggctgaaaaa caggcggcgg ttgaaaccgg cgaaatgacc atcgagcgct
     5281 ttattgatgg tctttacgcg gaagtttccc gcctggttga gagcgccgat attgccatca
     5341 gcgcgacgga aaaacatccc gttaaaacgg cgcttaaccg cctcgcggtt aaatgtccgt
     5401 cgtgcggttc tgagctggtt atgacgccga aaacctgcgc ctgtacgggc tgtcagttca
     5461 aaatatggct ggaagtgcgc ggcaaaaagc tgacggaaaa acaggccgag aagttaatcg
     5521 gcaagggcaa aaccgacgtt attaaaggtt ttaaatcgaa gaaaaacgac gacacctatt
     5581 cgatgatcgt cacgctgaaa aacgcgcaaa ccggggaact cggttttgaa tatccaccac
     5641 gcgatccgct gaaaggattg aacgtatcta tgccgaagga gctgaaatta tgaaccgtat
     5701 ccaggacgaa ttaacctcag agcgcgcgct gaaaaagttt ttacgtgaaa gtgattttgc
     5761 ctttctggag atgctgagtg cacgtatttc agcagcgctt gatgaaaaac gcgcagagca
     5821 tatcgagcag gaaaagctac gccgacagcg ggaagaaaag cgcaatgaat tgctggcgct
     5881 gattgaatcg gagggctttt caccgacaga gctggttagt acaactcccg ccaaaaagtc
     5941 ggcaaaacgc aaagctaaat atcagtattt tgaaaacggc gtgcataaaa aatggtcagg
     6001 cgtcggacgc gtgccggttg taattcagca agaacttgac gccggaaaac ctcttgaatc
     6061 cttccttatt accaccgact aacaacggca cagggtcggt tttgacctgg cttttcgggc
     6121 ttcgaagact ggcgagcatg gcgattaaat tttgaaaaaa ttgctaatcg acaggccgcc
     6181 tgagtctgcg aagtggcagg aggcgagtaa attcaatttc gaagaaattg ctaatttgcg
     6241 tcgccgattc cactttttga gcgctcaccc caagtgagcc gaaaaaagcc ggggggggcc
     6301 gaataaattt tgccgcccca gcgagcaaaa agacgcgccc acgcgtcggt ttatttgggg
     6361 ggcttgcccc ccacaccgtc gtgcctaagt ctccggccaa aagccggtag cgtggcgcga
     6421 ccacccccta aaaagcctag tttcccttta attcgtcttg tgaggaacga acaaggcgaa
     6481 gaaaagggct aaaacaggcg tcttttaggg ggtggtcgcg cgtagcgcgc gacggtgtgg
     6541 cctcttttga ccttgggggt tttggccggg cgttcagcca ggacattacc cccatgcgac
     6601 atgcagtaag ggcgtccagc ccgcgcgtaa tgccgcacgc cgttagatcg cgggagtgta
     6661 cctcctgcga tcatggccgc ttagcggcca cttcttttgc agcatggaat cggtcagtca
     6721 gaacgatcac aacctgtgac cgttctgact gaccgatggt gctgaccgaa tttaagtgaa
     6781 ggagaatatc gtgatattcg acaaactgaa tcatttgctg tcgccgatag caaatgccgt
     6841 cctggccttt atcgcctttt tgcaggcgca tcaactggcg ttagcgctgc tgtccggcgt
     6901 catgctgcct tttatatttt ctgcgaaaca cggcggtcag gaaaagccga cgtttctgca
     6961 aaggatctta attctgcttt ccatgatctg ttttgttttc ggctcgatag cgccagtggc
     7021 cgtctggttt ttacagcgca tttatggccg ggaagatatc gccagtccgc cgctgcttac
     7081 ctggacaatg gcgctactgt ttaccgtcgc cggttttatt ctgcacgtgg ttttacggcg
     7141 tttgatctcg cctgaatttg ataaggtcaa atgcggcatg gtgaaaaaaa ccagcatgga
     7201 gcgcgaccgc agaaccgacg ttcgccgggt aaaagaaatt ttacccgtca ccacagaata
     7261 cgatccgatg gattacatcg atctgaataa ggggattttt atcgggctga gccgcgaggg
     7321 tgagccgcaa tatattccgt taaaagaatg gcagacccag cacgccgaca ttatcggcac
     7381 cactggtgcc ggtaaaggcg tcgtaaccgg tattctgctt tatcagagca tcctcgccga
     7441 cgagggcgtt tttgttctcg accccaaaaa cgacgagtgg gcaccgcacc tgtataaaaa
     7501 agcgtgcgcg gacgccggaa aaccctttgt gcttatcgac ctgaataaac aggaatacca
     7561 gatgaacctt atcgacggta tcaccgccga ccatctggag gaattattcg tggccggttt
     7621 cagtctggcg gaaaaaggcg aggcggcgga tttttaccgt atcgacgacc gcaaggcggc
     7681 gagaacggcg gcgcagttcg tgaaggataa tccgggcgca accgtgcggg atatttataa
     7741 cggcgattat gttcagggca ttggcgagac gattaaagcc tttcacggta aaaccgagga
     7801 gctggcaatg ctcaacagca ttaacgcgcc aggcggtttt gcgctgaagg agattttcga
     7861 caagggcggc tgctgctata tcatcggttc gatgcgtaac tctaaaatca ttaccgcgca
     7921 gcgcatgatt ttagttcgcc tgttccagct cgcggaattg cgcgaccgcg tcgcggggaa
     7981 tatccgcccc atcgccatct ttaaggacga ggtgaaatat cacctgtcca aaccggcgct
     8041 cgaagggtta ggcgcggcac gcgataaagg cgtccatata ttaatggcgc accagtcaat
     8101 tgccgacctg cgcgactgtc cggcggattt aaacggcgac gccgtggttg gggcggttgt
     8161 ggaaaactgc aagtttaagc tggtatataa acttcaggat ccagacaccg ccgaatggat
     8221 tgcgcgcatg tcgggcacga ttttagtcga tgatgaaagc cggaaagtca ccaccacggc
     8281 ggtgctgaca gaaaaagtcg ataacgagcg catgcttcgg caggcggaaa gctattacat
     8341 cgacagcaat atgctccaga acctgccgaa gttcgtcagc tatatcttca gccagaacgc
     8401 gctcgcgttc ccgtcgttaa tctcaccgct caaggttaaa aaagccccac tggagctgtt
     8461 taccctgccg ccggtcgcag tcgctacggt ggcagaagtt aaaccggtgc tggatctgga
     8521 ttttgataaa acggctccgg cagttccggc acctgacatt attccggtca gcgcaacaac
     8581 caccaaaaaa ccctcagcaa cgccaatacc cactccgccg gtgcaacctg acgaggagtg
     8641 gctgatggca tcacagcccg ctccaaatga cgaaatacat tcgttgctgg atttataaaa
     8701 taaggagttt ctatgcttat ttcgtcgtat caggaacgtt atgcacgcag cactgaaaaa
     8761 attcgcgtac tgctgaattt tctcaaggag gaaacctaca gcgattttca aatcataaag
     8821 ctgctttttg attttaaatc tgaccgccct ctctaccggt tgttggagaa ggtggagaaa
     8881 atggggctga ttcagaagca cgtgcatgaa tcccgcgcgt cgagaatatc cctgtggggc
     8941 attaccaccg acgggctggc cgttatcctc acggcggacg acaacataat gcccgcccgc
     9001 tttgaaccct accgcctgac cggctggtca ctcgaccatc accttgataa ccagctagtg
     9061 cgccttacgc tggagaagaa cggcgcgaca gggtggatta acggcgaccg ttcaacgttc
     9121 ctcagtcagt acagcgtcaa acaccgtccc gacgggctta tcagcctgcc ggacggcagg
     9181 ctgatcgccg ttgaaaccga acgacgtatc aaaacaaagg cgcgctatca gtccatcatg
     9241 gcgagccacc tgctggctat cagcgataaa aaatggcggt acgcgtttta cgtcgccccg
     9301 gacgaggcga aaaagcgcgc cctgaagatt attttcgaca gcatcgccag cgtgatcgtg
     9361 aaaaaccagc atgtgcaact ggaggcaaaa caccgcgacg ttttccgctt ttacaccctc
     9421 gatgaactga aaaacctgga gctggacaac tatgcgtaaa accacccttc tggtgctggc
     9481 cgccgccctc tgcgtgc
//
LOCUS       DAJVRL010000126         9494 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVRL010000126 DAJVRL010000000
VERSION     DAJVRL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.126"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..190)
                     /locus_tag="OQN02_003409"
     CDS             complement(<1..190)
                     /locus_tag="OQN02_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCS1644085.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="OQN02_003410"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="OQN02_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1644086.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="OQN02_003411"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="OQN02_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1644087.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="OQN02_003412"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="OQN02_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1644088.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="OQN02_003413"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="OQN02_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="OQN02_003414"
     CDS             complement(3262..3381)
                     /locus_tag="OQN02_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1644089.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="OQN02_003415"
     CDS             complement(3342..3527)
                     /locus_tag="OQN02_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644090.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="OQN02_003416"
     CDS             3628..3801
                     /locus_tag="OQN02_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644091.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="OQN02_003417"
     CDS             3803..4147
                     /locus_tag="OQN02_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1644092.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="OQN02_003418"
     CDS             4151..4486
                     /locus_tag="OQN02_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1644093.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="OQN02_003419"
     CDS             complement(4543..6864)
                     /locus_tag="OQN02_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1644094.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="OQN02_003420"
     CDS             complement(6981..7190)
                     /locus_tag="OQN02_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644095.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="OQN02_003421"
     CDS             complement(7941..9464)
                     /locus_tag="OQN02_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1644096.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2660 a         2203 c         2080 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa taca
//
LOCUS       DAJVRL010000127         9399 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVRL010000127 DAJVRL010000000
VERSION     DAJVRL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.127"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OQN02_003422"
     CDS             complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OQN02_003422"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1644097.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1685..3493
                     /gene="frdA"
                     /locus_tag="OQN02_003423"
     CDS             1685..3493
                     /gene="frdA"
                     /locus_tag="OQN02_003423"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1644098.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3486..4220
                     /gene="frdB"
                     /locus_tag="OQN02_003424"
     CDS             3486..4220
                     /gene="frdB"
                     /locus_tag="OQN02_003424"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1644099.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4231..4626
                     /gene="frdC"
                     /locus_tag="OQN02_003425"
     CDS             4231..4626
                     /gene="frdC"
                     /locus_tag="OQN02_003425"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1644100.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4637..4996
                     /gene="frdD"
                     /locus_tag="OQN02_003426"
     CDS             4637..4996
                     /gene="frdD"
                     /locus_tag="OQN02_003426"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1644101.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5059..6192
                     /locus_tag="OQN02_003427"
     CDS             5059..6192
                     /locus_tag="OQN02_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1644102.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6281..6814
                     /gene="blc"
                     /locus_tag="OQN02_003428"
     CDS             6281..6814
                     /gene="blc"
                     /locus_tag="OQN02_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1644103.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OQN02_003429"
     CDS             complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OQN02_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1644104.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OQN02_003430"
     CDS             complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OQN02_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1644105.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OQN02_003431"
     CDS             complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OQN02_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1644106.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OQN02_003432"
     CDS             complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OQN02_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1644107.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8346..9374
                     /gene="epmB"
                     /locus_tag="OQN02_003433"
     CDS             8346..9374
                     /gene="epmB"
                     /locus_tag="OQN02_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1644108.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2377 a         2422 c         2416 g         2184 t
ORIGIN      
        1 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
       61 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
      121 aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt
      181 tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt
      241 aaaaaccaat acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc
      301 tttaacggaa aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg
      361 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
      421 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
      481 agtcaggcat accaactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
      541 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
      601 ccgttaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
      661 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
      721 ggtacacaaa ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca
      781 attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg
      841 ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca
      901 agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga
      961 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca
     1021 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     1081 ggaataccgg cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca
     1141 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     1201 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     1261 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag
     1321 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     1381 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa
     1441 cagcggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     1501 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     1561 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     1621 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     1681 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     1741 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     1801 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     1861 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     1921 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     1981 gtggggatgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     2041 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     2101 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     2161 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg
     2221 cacgttggtg cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt
     2281 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
     2341 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg
     2401 ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa
     2461 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
     2521 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
     2581 gcgtaaaggc aacaccatcc ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca
     2641 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
     2701 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat
     2761 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
     2821 tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga
     2881 actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
     2941 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
     3001 tctagttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc
     3061 aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
     3121 gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt
     3181 caacaccgac ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat
     3241 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg
     3301 ttgcaccgag cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga
     3361 tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     3421 cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa
     3481 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     3541 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     3601 atgcgctggg ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc
     3661 gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     3721 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     3781 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     3841 cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     3901 agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     3961 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     4021 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     4081 agaacggtgt atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg
     4141 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     4201 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     4261 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     4321 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
     4381 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     4441 atcattaacc tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg
     4501 gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     4561 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     4621 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     4681 gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct
     4741 ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt
     4801 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     4861 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc
     4921 tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg
     4981 tgtcgttaca atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt
     5041 gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct
     5101 tactccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
     5161 cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct
     5221 tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg
     5281 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
     5341 gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct
     5401 aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca
     5461 ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
     5521 cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc
     5581 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc
     5641 ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat
     5701 tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct
     5761 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
     5821 cccccccttg atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc
     5881 tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc
     5941 aatcctgaca gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta
     6001 aaagcgatta agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
     6061 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
     6121 ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac
     6181 gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc
     6241 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
     6301 tgccgcagcg acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg
     6361 cgtgaccgta gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc
     6421 ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg
     6481 tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca
     6541 gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc
     6601 attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca
     6661 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
     6721 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
     6781 taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat
     6841 ccccaatacg attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag
     6901 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
     6961 ccctaccggt aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt
     7021 cacggtaata acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc
     7081 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
     7141 aaatttgggg ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg
     7201 ttgaacggat attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc
     7261 agaaaaaaca aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg
     7321 ttgctgcgcc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg
     7381 tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct
     7441 tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa
     7501 taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa
     7561 ttagctggca gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca
     7621 agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt
     7681 catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta
     7741 cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg
     7801 tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt
     7861 acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt
     7921 cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc
     7981 cagcagccat ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt
     8041 ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc
     8101 gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc
     8161 ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa
     8221 ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa
     8281 atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc
     8341 acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac
     8401 ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg
     8461 aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat
     8521 ttatcgatcg catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct
     8581 cgcaagatga gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca
     8641 gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg
     8701 gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg
     8761 gcaacaagcg taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg
     8821 agatgatttt ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc
     8881 tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga
     8941 ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc
     9001 aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg
     9061 cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg
     9121 gtgtgaacga taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg
     9181 tgatgccgta ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga
     9241 gtgatgacga agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg
     9301 tgccgaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc
     9361 tacgccagca gtaactgatt aaacagggaa gttaaacat
//
LOCUS       DAJVRL010000128         9371 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVRL010000128 DAJVRL010000000
VERSION     DAJVRL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.128"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..268
                     /gene="mliC"
                     /locus_tag="OQN02_003434"
     CDS             <1..268
                     /gene="mliC"
                     /locus_tag="OQN02_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS1644109.1"
                     /translation="QIQVERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLK
                     QGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            327..983
                     /gene="pdxH"
                     /locus_tag="OQN02_003435"
     CDS             327..983
                     /gene="pdxH"
                     /locus_tag="OQN02_003435"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1644110.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1112..2386
                     /gene="tyrS"
                     /locus_tag="OQN02_003436"
     CDS             1112..2386
                     /gene="tyrS"
                     /locus_tag="OQN02_003436"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1644111.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2445..3308
                     /gene="pdxY"
                     /locus_tag="OQN02_003437"
     CDS             2445..3308
                     /gene="pdxY"
                     /locus_tag="OQN02_003437"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1644112.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3352..3957)
                     /gene="gstA"
                     /locus_tag="OQN02_003438"
     CDS             complement(3352..3957)
                     /gene="gstA"
                     /locus_tag="OQN02_003438"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1644113.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4063..5565)
                     /gene="dtpA"
                     /locus_tag="OQN02_003439"
     CDS             complement(4063..5565)
                     /gene="dtpA"
                     /locus_tag="OQN02_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1644114.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6176..6811)
                     /gene="nth"
                     /locus_tag="OQN02_003440"
     CDS             complement(6176..6811)
                     /gene="nth"
                     /locus_tag="OQN02_003440"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1644115.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6811..7506)
                     /gene="rsxE"
                     /locus_tag="OQN02_003441"
     CDS             complement(6811..7506)
                     /gene="rsxE"
                     /locus_tag="OQN02_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1644116.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7510..8130)
                     /gene="rsxG"
                     /locus_tag="OQN02_003442"
     CDS             complement(7510..8130)
                     /gene="rsxG"
                     /locus_tag="OQN02_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1644117.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8134..9192)
                     /gene="rsxD"
                     /locus_tag="OQN02_003443"
     CDS             complement(8134..9192)
                     /gene="rsxD"
                     /locus_tag="OQN02_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1644118.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9193..>9371)
                     /locus_tag="OQN02_003444"
     CDS             complement(9193..>9371)
                     /locus_tag="OQN02_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644119.1"
                     /translation="RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARV
                     QAKKAAQQKVVNED"
BASE COUNT         2396 a         2348 c         2382 g         2245 t
ORIGIN      
        1 gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg atgaaaaacc
       61 gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg ataatcaact
      121 actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg ggatctatgt
      181 tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg tcttgaataa
      241 ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac aatagcgtca
      301 cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag caaatcgcgc
      361 atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc gccgatccat
      421 taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg gaccctaccg
      481 cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc gttttactca
      541 aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt aaagcacatc
      601 aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt gaacgccagg
      661 tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa tattttcata
      721 gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt cgcatttctg
      781 cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa cagggcgaag
      841 tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt gagttctggc
      901 agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat gatgcgtgga
      961 agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg ctggtactcc
     1021 tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa
     1081 aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga ttaaacaatt
     1141 gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag agcgactggc
     1201 gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct tgcatttggg
     1261 gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca agccggttgc
     1321 gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa
     1381 gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc aggttgcccc
     1441 gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact atgactggtt
     1501 cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct ccgttaacca
     1561 gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt
     1621 cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga acaaacagta
     1681 cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt ctggtatcga
     1741 cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc tgatcaccaa
     1801 agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg atccgaagaa
     1861 aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg acgtttaccg
     1921 cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag aagaagaaga
     1981 taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg tgactcgtct
     2041 ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc tgttcagcgg
     2101 ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg gcgtaccgat
     2161 ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg aactgcaacc
     2221 ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta acggtgaaaa
     2281 acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc gttttacctt
     2341 actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca ttaagtggaa
     2401 agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg aaaaatattc
     2461 tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca gagtttccga
     2521 tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct aatcacaccc
     2581 aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag
     2641 gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc tatctgggat
     2701 cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca gcgaatccgc
     2761 aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac
     2821 cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc attgcgccga
     2881 atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc
     2941 tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa cacctggcgc
     3001 gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat gaagtctggc
     3061 atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga
     3121 cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag gccctggaac
     3181 atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc
     3241 tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc agcgcaacaa
     3301 agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct attactttaa
     3361 gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt gcataaatgc
     3421 cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc gcagaaccgt
     3481 aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct catccttcag
     3541 tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg gtttgtactc
     3601 ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg tggcgatgta
     3661 attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca gcaactggcg
     3721 gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca gcaaagtacc
     3781 atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt aatcgtcacc
     3841 gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct ttccgctctc
     3901 acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca atttcatcgg
     3961 taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg
     4021 ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta cagtagcttt
     4081 cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg cggtcagcag
     4141 catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc gaccatagac
     4201 ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac ccgcaacata
     4261 accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa tgaagcccat
     4321 cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga tcatcagttc
     4381 cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga tacctgcgtc
     4441 agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca tcaccatgcc
     4501 gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga tagcggccag
     4561 aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct caggttctac
     4621 ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca gtgacgttgg
     4681 catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga aggcaacgat
     4741 cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga taaccacaat
     4801 accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca gtagccaggt
     4861 ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac ggtagttgac
     4921 tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga aggcgaagtt
     4981 aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc agccgtattt
     5041 cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga cggacatgta
     5101 gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg tagaaagcag
     5161 agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca tacccatata
     5221 aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg ccagcacaat
     5281 agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc agccgccgat
     5341 agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg aatccgcttc
     5401 agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc cgtaataacc
     5461 aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt gtttgaaagc
     5521 gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta cctctttttt
     5581 tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt
     5641 gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc agacaagtcc
     5701 tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg ggaaatgtta
     5761 tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat tgagaggggg
     5821 atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga gatagtccag
     5881 tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt gtgtaaattt
     5941 aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg tgatcttgat
     6001 cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt ttggttatcc
     6061 gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta aaaagatgtt
     6121 ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct tttcttcaga
     6181 tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca cagcggggct
     6241 tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag tcgactttaa
     6301 actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt ttccccggcg
     6361 caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc
     6421 cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc agggcttcaa
     6481 gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata cggcaggttt
     6541 tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag gttttcaccc
     6601 cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag agtttcgccg
     6661 tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca atcagcaatt
     6721 caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca cgcaggcgag
     6781 tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac cgtttggcaa
     6841 tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa tcaggtattt
     6901 tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg ccagcaggaa
     6961 aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac ctaacagcgc
     7021 atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac ccagcacgaa
     7081 catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa gcgccggacc
     7141 ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga caatcagcgg
     7201 aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca gcatctgtac
     7261 agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct cggctggcgt
     7321 ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag
     7381 tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac aaaggccgag
     7441 cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt ctttaatttc
     7501 gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta acgtctgagc
     7561 gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt
     7621 gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat ccgcaccact
     7681 gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga ttttatcgcc
     7741 aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc cattaaaatc
     7801 ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg cttccagaac
     7861 ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac ctttacctaa
     7921 ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc gttcggctgg
     7981 cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa ttgtcgtttt
     8041 ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata gtgccagtgt
     8101 aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg
     8161 cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga cggcaaaagc
     8221 cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc ccgccagcgc
     8281 gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag tcaaaataaa
     8341 gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg ccagtgtttc
     8401 tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga agctgagggg
     8461 aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca gccaggcgag
     8521 atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat attgcataat
     8581 ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg gtgtcgcctg
     8641 actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg cggtatgacc
     8701 gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg
     8761 cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat aaccaatcat
     8821 tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga tgatcaccgc
     8881 aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac ttaccgccag
     8941 caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg actgcttgcg
     9001 tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca ggatctgaac
     9061 gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg ctgcgagcaa
     9121 caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg agctagctat
     9181 tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt tggcctgaac
     9241 gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt ctggttccgc
     9301 attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg cttcgacggc
     9361 agctttgcgc g
//
LOCUS       DAJVRL010000129         9255 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVRL010000129 DAJVRL010000000
VERSION     DAJVRL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.129"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OQN02_003445"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OQN02_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1644120.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OQN02_003446"
     CDS             1828..3147
                     /locus_tag="OQN02_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1644121.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OQN02_003447"
     CDS             3445..3486
                     /locus_tag="OQN02_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644122.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OQN02_003448"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OQN02_003448"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCS1644123.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OQN02_003449"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OQN02_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OQN02_003450"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OQN02_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OQN02_003451"
     CDS             5815..6711
                     /locus_tag="OQN02_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1644124.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7694
                     /locus_tag="OQN02_003452"
     CDS             6708..7694
                     /locus_tag="OQN02_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1644125.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7687..8613
                     /locus_tag="OQN02_003453"
                     /pseudo
     CDS             7687..8613
                     /locus_tag="OQN02_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OQN02_003454"
     CDS             complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OQN02_003454"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1644126.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2265 a         2260 c         2318 g         2412 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggagca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 aaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctgccgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctaggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7621 cgctgacagc gtgtggtgac aacaaccagc actgcgcctg ttggtatccg cagcaggagg
     7681 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt tccggcccgt
     7741 aaaaactggc ttggtaaatc tacggaacat gttcatgcca ttaatggtat tgatttacag
     7801 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7861 gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg ttcaggctca
     7921 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
     7981 gtgtggcgca tcatcacaga atcgctctgg atagctaagc gtagtagtga acaacagcgg
     8041 cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     8101 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8161 tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat ctccgtgcag
     8221 gcgcagatcc tcaatttgct ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
     8281 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8341 gggcagattg tagaactggg cgacgcgcaa caggtgttga ctgcttcggc acatccatac
     8401 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgagca
     8461 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8521 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattagcaat aagggaggac
     8581 ggacgtgagc tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8641 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8701 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8761 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8821 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatt gatgttggcg
     8881 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8941 cgccaatcag ttcttcaggg ttggttcctt tttcgccttc aaaacgcgtg ttaaatccat
     9001 acggctgttg gttcagcacg ccgctctcgg tggatactgt tcccttcccg cgtttgatat
     9061 cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
     9121 aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat attccgaata
     9181 aaatacggcg aaaatattac ttctgcactt aaattaaatt cccacaatag ataaccggga
     9241 atatatatct caaga
//
LOCUS       DAJVRL010000130         9223 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVRL010000130 DAJVRL010000000
VERSION     DAJVRL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.130"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            44..397
                     /locus_tag="OQN02_003455"
     CDS             44..397
                     /locus_tag="OQN02_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1644127.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="OQN02_003456"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="OQN02_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCS1644128.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="OQN02_003457"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="OQN02_003457"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1644129.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="OQN02_003458"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="OQN02_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1644130.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="OQN02_003459"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="OQN02_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1644131.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="OQN02_003460"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="OQN02_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1644132.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="OQN02_003461"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="OQN02_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1644133.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="OQN02_003462"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="OQN02_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS1644134.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="OQN02_003463"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="OQN02_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS1644135.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7649..>9223
                     /gene="hycC"
                     /locus_tag="OQN02_003464"
     CDS             7649..>9223
                     /gene="hycC"
                     /locus_tag="OQN02_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS1644136.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGS
                     VWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSN
                     SGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVS
                     VVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMA
                     ICKGDRLPSRSRGAAWV"
BASE COUNT         2018 a         2511 c         2515 g         2179 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgctg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agcaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 tatcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctggggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaatat cggcattatc ctgctggggc tggggctggg
     8521 gctgggcgct ggcgtaacgg gtatcgcgct cgaacaaccg gcgctgattg ctcttggcct
     8581 ggtcggtggt ctgtaccatc tgcttaacca tagcctgttc aaaagcgtac tgttcctcgg
     8641 ggcggggagc gtctggttcc gtaccggtca tcgcgatatc gaaaaactcg gtggtattgg
     8701 caagaaaatg ccggttatct ccatcgccat gttagtcggg ttgatggcaa tggctgcgct
     8761 gccgccactg aacgggtttg ccggggaatg ggttatctat caatccttct tcaaactgag
     8821 caatagcggc gcgtttgttg cccgtctgct ggggccgctg ctcgctgtgg gtctggcaat
     8881 taccggtgcg ctggcggtga tgtgtatggc gaaagtctat ggcgtcacgt tcctcggcgc
     8941 gccgcgtacc aaagaagccg aaaacgccac ctgtgcgccg ctcctgatga gcgtaagcgt
     9001 agtggcactg gcgatttgct gcgtaattgg cggtgttgct gcgccgtggc tgctgccgat
     9061 gctctctgct gctgtacctc tgcctctgga gcctgctaac accaccgttt ctcaaccaat
     9121 gatcacattg ctgctgattg cctgcccgct gctgccattc atcattatgg cgatttgcaa
     9181 aggcgaccgc ctgccgtcac gttcccgcgg tgcggcctgg gtg
//
LOCUS       DAJVRL010000131         9153 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVRL010000131 DAJVRL010000000
VERSION     DAJVRL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.131"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..923
                     /locus_tag="OQN02_003465"
     CDS             <1..923
                     /locus_tag="OQN02_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1644137.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OQN02_003466"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OQN02_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1644138.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OQN02_003467"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OQN02_003467"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1644139.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OQN02_003468"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OQN02_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1644140.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OQN02_003469"
     CDS             4446..4805
                     /locus_tag="OQN02_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1644141.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="OQN02_003470"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="OQN02_003470"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1644142.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="OQN02_003471"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="OQN02_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS1644143.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="OQN02_003472"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="OQN02_003472"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1644144.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2217 c         2469 g         2373 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     9001 gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgac
     9061 gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat gcttgactct
     9121 gtagcgggaa agcgtattat gcacaccccg cgc
//
LOCUS       DAJVRL010000132         9014 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVRL010000132 DAJVRL010000000
VERSION     DAJVRL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.132"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OQN02_003473"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OQN02_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1644145.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OQN02_003474"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OQN02_003474"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1644146.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OQN02_003475"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OQN02_003475"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1644147.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OQN02_003476"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OQN02_003476"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1644148.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="OQN02_003477"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="OQN02_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1644149.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="OQN02_003478"
     CDS             5545..6018
                     /locus_tag="OQN02_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1644150.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="OQN02_003479"
     CDS             6047..6589
                     /locus_tag="OQN02_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCS1644151.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="OQN02_003480"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="OQN02_003480"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1644152.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="OQN02_003481"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="OQN02_003481"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1644153.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAILSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="OQN02_003482"
     CDS             7986..8243
                     /locus_tag="OQN02_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1644154.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="OQN02_003483"
     CDS             complement(8219..8773)
                     /locus_tag="OQN02_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1644155.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2257 a         2187 c         2247 g         2323 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatgata aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgttttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcctg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaa
//
LOCUS       DAJVRL010000133         8989 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVRL010000133 DAJVRL010000000
VERSION     DAJVRL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.133"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            146..2330
                     /gene="espL4"
                     /locus_tag="OQN02_003484"
                     /pseudo
     CDS             146..2330
                     /gene="espL4"
                     /locus_tag="OQN02_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="OQN02_003485"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="OQN02_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="OQN02_003486"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="OQN02_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="OQN02_003487"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="OQN02_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="OQN02_003488"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="OQN02_003488"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1644156.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="OQN02_003489"
     CDS             8509..8952
                     /locus_tag="OQN02_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS1644157.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2357 a         2168 c         2166 g         2298 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatatta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAJVRL010000134         8920 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVRL010000134 DAJVRL010000000
VERSION     DAJVRL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.134"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(239..1210)
                     /locus_tag="OQN02_003490"
     CDS             complement(239..1210)
                     /locus_tag="OQN02_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HCS1644158.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
     gene            complement(1210..2376)
                     /gene="sopA"
                     /locus_tag="OQN02_003491"
     CDS             complement(1210..2376)
                     /gene="sopA"
                     /locus_tag="OQN02_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HCS1644159.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3117..4127
                     /locus_tag="OQN02_003492"
     CDS             3117..4127
                     /locus_tag="OQN02_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS1644160.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            complement(4933..5262)
                     /locus_tag="OQN02_003493"
                     /pseudo
     CDS             complement(4933..5262)
                     /locus_tag="OQN02_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
     gene            5380..6105
                     /locus_tag="OQN02_003494"
     CDS             5380..6105
                     /locus_tag="OQN02_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCS1644161.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            6205..6615
                     /gene="uraH"
                     /locus_tag="OQN02_003495"
     CDS             6205..6615
                     /gene="uraH"
                     /locus_tag="OQN02_003495"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1644162.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            6718..7677
                     /locus_tag="OQN02_003496"
     CDS             6718..7677
                     /locus_tag="OQN02_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HCS1644163.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            7823..8911
                     /locus_tag="OQN02_003497"
     CDS             7823..8911
                     /locus_tag="OQN02_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1644164.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT         2254 a         2358 c         2283 g         2025 t
ORIGIN      
        1 caggtgggac cgtggtccca ggcagataac ctgaccggcg atacaggtgg gaccgtggtc
       61 ccaggcagat aagctgaccg acgacgcagg tgggaccgtg gtcccaggga gataacctga
      121 gcggcgatac aggtgggacc gtggtcccag agagaatatt caggccagtt atgttttctg
      181 gcctgtgaga aaggaaatga agtaaaggga gatcaccgta gactaaagcg tgtccgcatc
      241 agactcctgg tttctcaagc tccttaagaa tggcttctat tttctctata cactccgcag
      301 gaatgcggga cctatccagg ttaagcacca ttttatcgcc cttatacaat gctgttgccc
      361 ctggagcaaa ctgatgacgt gagctcagat taactcttga tgcgggagat tgtttcagta
      421 cggatgttaa aagactaatg acctcttcag cttcaaagat tagtcccgct ttcttctgat
      481 cgtggagagt ctcagcctgc tgcttcagta attcttcttt atctgcaaaa gccttctgaa
      541 gggcttcacc tgaccgggca gacagttctc caggatgtgc aaacagggca acaacggatt
      601 taggcaattt ggccgtattg atacagcggg taatgatctt acgagagatg ttttccgcat
      661 cggcaagcgc agaaatattt cctgcaaact cattctgcag ccggcttgta taacgcaggc
      721 cacgttcata agcactcgtc ggccgataat cgttacccag tcgggacaat gcagccatct
      781 gctcatcgtc cagttcacca accagaacgc gataatcact ttccgtcagg attgcggctt
      841 tacggcgacg gctgccgtcg gcaatttcta tgacatcaga aacccggcgg ccgaaagctg
      901 gagtctgctg accggtcagt aaaaatgaag gaatgagatc atccagagca tcttcggtaa
      961 gtagttcctg atcgcgctca ttgcctgacc atacgcgtga agcgctctca acagagtctc
     1021 cccggagcac ctcaagggta aatttcactt cccgtccaca taccggaaga gagattgcat
     1081 taccgcgggc catagcaccc acgcgcgcaa ttaatgaatc caccatcggc gcggccggcg
     1141 ccggtgatga agtatcttca gtcgattgac tatgtgtggt atgtcttgga atgacaggag
     1201 cacgcttcat tatctaatct cccagcgtgg tttaatcaga cgatcaaaaa tctcattgca
     1261 aacaggctcc cagatagaga gggcattacg ccaggcaccg gttgaagaac gttgatcaat
     1321 agcctgttca aaaacggttc tcatacggat ctgaccttta ccgacttcat ccgtttcgcg
     1381 cacaacattt ttaaggacca tgcttcccca ggcatcccga atttgttctt ccatccacgg
     1441 ggactgtgag ccattattat tactgtattt ggtaagcaga atacggacat ctggctcgaa
     1501 gcctttaaga tccacgttct taagcaggtc acgaagcata tcgaaaaact gcagtgcgga
     1561 ggtgtagtcg aacagctctg ccggagtagg gacaatcaga acatcagcag cgcatacaac
     1621 atttatcgta ccgataccaa ggttgggtgc actgtcgata actatcacgt cataatcatg
     1681 agcgacagtt tcaatggcca gacggagcat caggtgcggg tcagcaggta atttgccttc
     1741 gtcaaatttc cccatcagct cggtttcaat gcggtgcagt gccagacagg acgggatgat
     1801 atcaaggcca ggccagcagg tgggctttat agcgtagctg gcgtcatcct tttccccaag
     1861 ataaaagggc aggagagtgt cctcagcatg aatatgaaga tcgggcaccc atccgtgata
     1921 catagaagca gttccctgtg ggtcattgcc ttcaacaagc agaacacgga gtcctttcag
     1981 agcaagatcc tgagccaggt gaacggatac tgaggttttg taaacaccac ctttgtgagc
     2041 tgcaacgcca atcaccggcg gaaacgcatc ttcagcacgg cgcagtcgtg taccaaatac
     2101 atcccgcatg tgattgattt gttcgatggt ataaccaacg cgttgctcaa ctctcccacg
     2161 ggtttccata tcagggtgcg gcagccggcc agctttctcg gcatccctga tagcctgaga
     2221 tgacacgcca actaaatctg ccgcttcacc aatcctccag cgccgggtta ttttccgggc
     2281 ttccggactg tcatcattaa actgtgcaat ggcgattgct ttcgtcattt catgaccagc
     2341 acttatgcac tggttgagtg tatccatgag tcccatactg aacatccttt aatcatcact
     2401 ttgcattatt ttattaaatc ttggaattta ttgcaaagca acaataaaat cgcaaagcac
     2461 acgaaaaagt gcaaagtgaa ccacattggg taatgaaaga acgcagggga ttacaaaaga
     2521 gtacataccg cagtcaatat taatcactgc cgctcgccgc agtaagcgat ctcacacagt
     2581 gagaaagcgg cgaggaagcg gaaaatcaca gaactcacag attgcacgta cgtcgggttg
     2641 ctaaagatga ctcgggatca gccagttata atgaaattga ctgcggtatg taatatattg
     2701 actatggtat gatgcttacc gcatctgtag gaaagacgac tcagcatacc gcagtcaatg
     2761 tttcattgtt gtacctgtag atctcaatct tccagagcgt attcaggaaa atcaggccag
     2821 gggtctggat atctggtgtg gcgcttccgg ccttgtatct gggttattca tggtctcgat
     2881 ccaataattg atcccaagga gatcatagtg agatccaaaa gagatccccg gatcgctcca
     2941 gcatgcgctg taactggctt taacgtctgt ctggatcgac tgcggaatgt aaaatgttga
     3001 ctgttatatg cataatctaa tactgtgata tgcaacggta tttactgagg tatgtatatc
     3061 cgttgactgc gatacgtatt actacatcct tttgtggata accgttgagg atcaagatga
     3121 cgagcgaaaa taatagctta cttctgaacc ttcaggaagt tgataagaca accggtgaag
     3181 ttgttaagct ggatgtcaac agcaccagta ccgtgcagcc agtagcgctc atgagactcg
     3241 gtctcttcgt cccaactctg aaatcaacag ggaagagcaa ggcgaaccgg aaaaacgtta
     3301 cagacgcgac tgaggagctt gtacagctgg ccattgccaa aagcgaagga tacactgacg
     3361 ttaagatcac cggttcgcgt cttgatatgg acacggattt taaagtctgg ctggggataa
     3421 ttcgctccat gtcggagtat ggggtaaaaa gtgacaccct ggaactgtcg ttcgtcgaat
     3481 tcgttaagat gtgcggattt gactcccgtc gttcaaacaa aaaaatgcgc gatcgcatca
     3541 gtaattccct gtttaaactc gcctcggtta cgctgaagtt ccagagcgag accaaaggat
     3601 ggaccaccca tctggtgcag tcagcctatt atgacatcaa cgaggatatc gttgagatca
     3661 aggctgaacc taagctgttt gagctttacc acatggacag aagggtactg ctgcggctga
     3721 aagccattga cgccctgcag cgcaaggaat cagcccaggc gctttatacc tacatcgaaa
     3781 gtcttcccca gaatcctgca cctatttcca tgaaacggat gcgtgaccgg ctgaatctca
     3841 cctcaaatgt ctatacacag aaccacacgg tgcgtaaggc gatggagcag ctgcgtgata
     3901 ttggttatct ggattacacc gagtttaaac gtggccgagc cacctatttc agcgtgcatt
     3961 atcgcaaccc caaactcatc agtagtcctg tgaaggttcc gcgcaaagag gaagaagaga
     4021 aggcgccaga gcaaaattat gacgaagtca ttaaggcgct gaaggctgct ggcatcgatc
     4081 ctcttaaact ggctgaagcc ctctcagcga tgaagccaga aaattgactg cgatatgcat
     4141 atcatgactg ccgtatgccg ctaaggacat tccattgact gtgatatgca tcacgcatac
     4201 cgcagtcaat atttattctc tatcatgtct gcccggatcg aacaatattg actgtcatat
     4261 gtgatttcgg gtcatgatac gcagatattg actacggaat gcgggtttaa tgggggtaat
     4321 ccctggtctg ttcaccattc catgggggat aacatgccac agtcaatttt tcagtaacgc
     4381 tctatatccc ttacgaaaat ctcaatcggg tgctgagacg gtcatgttac ctgacggtat
     4441 ccgttcccct gtgtactgca ggtttagccc ggcaggatga gactgcagct gtctgtataa
     4501 cccggcgcca gcttcacact gttaaatgcg atcaggaaac gggagccgaa tggcagaaag
     4561 gtgttggcta gggaaggtgc gaataagcag gttatttctt cccaagctga ctcgctgatt
     4621 aaaatttcgc ggatctgggc cgattttttt cccgcaaaca catcgaatca gcctatttag
     4681 gggtcagttt ggagatcgga aatttttgta cgttaagcac attatttaac caattcggat
     4741 acgtaagtga ttgatttatc gtcattggct atcgctttta ggttgtcgct cttgctgtat
     4801 cgcatttagg ttatccgatt aagacaaaat cccctgaaaa cgctgtatag cgcgggagta
     4861 caccctattg ctacaggtaa gcctgttcaa aaaacaggct taccgtacaa taattctcta
     4921 tatccaaact gaccccaaga aggacacctt cagcgcggcc gtggtcggtt cgccggtaaa
     4981 gtaggcttta ccctcgctct cattccactt gttcttcaca gggtcgtagc cgcggttaag
     5041 cacgctgatg ccgccgtcgc tgcgcttgcc gtaggtggcg gtaacctgct ctaggccgcg
     5101 ttcaaagcgg ttttccaggc gagcgacttc gtaccatttg ccaagatagc ggctggcgtc
     5161 aaaaccggaa atgggctgca cgcccttcgg tggggtaggg gatttacagg ctaccagcgt
     5221 cagggcaatg gcaacaccgg tcacaacagg ccatagcttc atgagaattc ctttcatttg
     5281 cacggttgga aattaagtgt agtgcaccgc ggcggcgctt catcccatcc gcgaaaattc
     5341 gtagtatatt gagagtattc ctcaactctg gacacggaca tggcttactc catcggcgaa
     5401 ttcgccagac tcagcgggat caccgccacc acgctgcggg cctggcagcg gcgctacggc
     5461 ttactcaagc ctcagcgcac ggagggcggc caccgccagt acagcgatga ggacgtccag
     5521 caggcgctaa aaatcctcga ctgggtaaaa aaaggcgtcc cgattggcca ggtcaaaccg
     5581 ctgctggagc gccctacgtc gcgcctgacc aacaactggc tcacgctgca gcagagcatg
     5641 ttacagcgcc tgcaggacgg caaaatcgag tccctgcgtc agatgattta cgacgcagga
     5701 cgcgagtacc caagaccaga actggtcgcg aacgtgctgc gtccgctgcg cagtcaggtg
     5761 tcggcaaacg tcgccgccgc catgaccctg cgggagatcc tcgacggcat catcatcgcc
     5821 tacacctcgt tttgcttgga aggcgataaa aaagcgccgg gcgacaacat cctgctcagc
     5881 ggctggaacc tgaatgaccc gtgcgaagtc tggctcgaag ccttaacccg caccgggcaa
     5941 ggccaccgta tcgatatttt acccacgccg cccgccacgc tggccccgga gattttcccg
     6001 gaccgccact ggcttctggt cacgcgcggt aaactcaccg ccgcgcgcaa aaagcagatc
     6061 gagcagtggc agcagcaggt atcgcttgaa gtgattatcc tctaatcatt ttctcctgcg
     6121 ggaatcttca cgaaaagttg aatattttcc cgcgccacaa atgctaacgt tttcctgtga
     6181 ccgacaaccc cacaggaaaa cacaatgaaa aaaactgcct ctctgctgat gctgaccacc
     6241 ctggcctttg cccctgccgc ctttagcgcc cctgccggca cgctgagcgt acacattctt
     6301 gaccagcaaa ccgggatgcc gccatcggac gttaccgtca cgctggaaaa acagcagcag
     6361 gacaaatgga ccccaattgc cagcggcaaa actgaccatg acggccgcat caaatcgctt
     6421 tatccggagg atcaggatat gcagcccggc gtctataaag tggcctttaa aaccgcagat
     6481 tacttccacg ggaagaagct ggactccttc ttcccggaga tcccggtgct gttcaccgta
     6541 acccggacca acgaaaagct gcacatccct ctcctgctga gccaatacgg ttattcaact
     6601 tacaagggca gttaagaaac gttgatttta aaggcaaata aaaaataatt ccgctaatac
     6661 ttggacaaat tagttatgtt gtgtaagttt taaagagaag aacgggagga ccacaccatg
     6721 acaacaaaac ctgtgctcgg tattagcgga tgtttgaccg ggtctgccgt tcgcttcgac
     6781 ggcggacaca agcgtatggg ctttgtcatg gatgaactgg cccagtgggt gagtttcagg
     6841 cccgtctgcc cggaaatggc gattggcctg ccgacgccac gcccggccat acgcctgacg
     6901 ctgacggaca gcggcgaaac gcagctccgc ttcagcaagc cgccccatga tgatataacg
     6961 cagaaaatgg ccgactttac ggcggactat ctgccaaaaa tcggcgatct gtcgggattt
     7021 atcgtctgcg cgaaatcgcc aagctgcggc atggaacgcg tgcggctata tgatgaaaac
     7081 ggcaaccggg gccgcaagga gggtgtcggg ctgttcaccg ccgccctgct tgaaacctac
     7141 ccgtggctgc ccgtggagga agacggtcgg ctgcacgacc cggtgctgcg ggaaaacttt
     7201 atcgagcggg tatttgccct gcacgagctg aacacgctgc gcgctaaagg cctgacgcgc
     7261 cgcgcgctgc tggatttcca cagccgctat aaattgcagc ttctggcgca ccatcaggcg
     7321 ggctaccgcg agattggccc gttcgttgcg tcgctgcatg aatgggaaga tctggacgcg
     7381 ttcttcgtgg cgtaccgcga aaagctgatg accatcctga aaaagcccgc ctcgcggaaa
     7441 aaccatacca acgtgctaat gcatatccag ggctatttcc gtaaccagct gaacagccgc
     7501 cagcgcgtcg agctgcgcga tgtgatcctg cactatcgcg acgggctgct acccatcctc
     7561 gcgccgttga cgctactgaa acactacatg gccgaatacc ctgaccggta tctgatgacg
     7621 caaaactact ttgatcccta tcctgatgat ttgggtctgc gtctggcagt aacctgacga
     7681 taaaaatgcg aaggcccgtt tgacccacag aggcccaaag cagtaccccg tttagacgac
     7741 accctcactg tcgtctcttt tttgcattcc acacaaaaga ctgtaattat gtacaggatt
     7801 gtaacgcaaa aagggactgc ctgtgatcac caccctgcac attcaaaact accgctcgat
     7861 tcgcgaaatg tcgctggagc tggaacagct caacatcgtc tttggcccaa acggcaccgg
     7921 aaaatccaat atctacaagg cgatccacct gatgcacagc gccgcacagg ggcagttttc
     7981 gcaggcactg gcgaacgagg gtgggatttt aaaagtgttc tgggcaggca aaacccgcag
     8041 cgaccagctg cggcggatga atttggccgt tgaaaccgaa acctacgaat acgagctgca
     8101 ggtaggcttt gtcgaaaaac tgccttaccc ctcacagttt caactcgatc cggtgatcaa
     8161 agaagagtcc atctggctga gtggtcaata ccgccgcccg tcgtcacagc tgatgaagcg
     8221 taaaaatcag gccgtatttc tgaacaacgt gcatcacgag aaagtcaccc acagcggcac
     8281 gctgtatgag aacgaatcgg tgttcggcca gcttggcgag ccgcatcttt acccggaagt
     8341 gtcgcagatg cgcgagtcgc tgcgtaactg gcgcttttat caggagtttt ctgtatcaat
     8401 cggctcggcc atgcgcgcgc ctcaggtcgg tttccgctcc ccagtgctgg ccagcgacgg
     8461 cgcaaacctg gcggcggcgt ttcagaccat cgtggaaatt ggcgacgagc tgctgctgat
     8521 gcgcatcctc gaccaggcct tccccggctg cgtgttttac agcgacaaca ccggcggacg
     8581 cttccggatg atgatgcagc gtgaagggct aagcagaccg ctggagccgg cggaattctc
     8641 cgacggcact ctgcgttttc tgtgcctggc ggtggcgcta ttaagcccgc gtccaccggc
     8701 ctttatcgcc ctcaacgaac cggaaaacag cctgcatccg cagatgctgc ccgcgctggc
     8761 gagcttaatc gccgaggcca gccgctactc gcaaatttgg ctgaccagcc actcgccgga
     8821 actggcgaat ttaattgaga aacagagatc gttttcgctg tatcagctgt cgatggtgga
     8881 aggagaaacg aaggtggaga ggctggggta gtctcccaga
//
LOCUS       DAJVRL010000135         8898 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVRL010000135 DAJVRL010000000
VERSION     DAJVRL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.135"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..2887
                     /locus_tag="OQN02_003498"
                     /pseudo
     CDS             1..2887
                     /locus_tag="OQN02_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OQN02_003499"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OQN02_003499"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS1644165.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="OQN02_003500"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="OQN02_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OQN02_003501"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OQN02_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="OQN02_003502"
     CDS             complement(7280..8041)
                     /locus_tag="OQN02_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCS1644166.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="OQN02_003503"
     CDS             complement(8038..8697)
                     /locus_tag="OQN02_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1644167.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2319 g         2048 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggttt
//
LOCUS       DAJVRL010000136         8761 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVRL010000136 DAJVRL010000000
VERSION     DAJVRL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.136"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..1929)
                     /gene="glnE"
                     /locus_tag="OQN02_003504"
     CDS             complement(<1..1929)
                     /gene="glnE"
                     /locus_tag="OQN02_003504"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1644168.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVS"
     gene            complement(1952..3253)
                     /gene="ygiF"
                     /locus_tag="OQN02_003505"
     CDS             complement(1952..3253)
                     /gene="ygiF"
                     /locus_tag="OQN02_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1644169.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            3495..4115
                     /locus_tag="OQN02_003506"
     CDS             3495..4115
                     /locus_tag="OQN02_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1644170.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            4179..5417
                     /gene="cca"
                     /locus_tag="OQN02_003507"
     CDS             4179..5417
                     /gene="cca"
                     /locus_tag="OQN02_003507"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1644171.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(5580..6401)
                     /gene="bacA"
                     /locus_tag="OQN02_003508"
     CDS             complement(5580..6401)
                     /gene="bacA"
                     /locus_tag="OQN02_003508"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1644172.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(6492..6860)
                     /gene="folB"
                     /locus_tag="OQN02_003509"
     CDS             complement(6492..6860)
                     /gene="folB"
                     /locus_tag="OQN02_003509"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1644173.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            6965..7582
                     /gene="plsY"
                     /locus_tag="OQN02_003510"
     CDS             6965..7582
                     /gene="plsY"
                     /locus_tag="OQN02_003510"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1644174.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(7595..8526)
                     /gene="ttdR"
                     /locus_tag="OQN02_003511"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(7595..8526)
                     /gene="ttdR"
                     /locus_tag="OQN02_003511"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         2104 a         2471 c         2162 g         2024 t
ORIGIN      
        1 gctcactttc attaccggta gcgtaccggc gatatccgct gccgcaatgc gtaacagctg
       61 cgcctgtttg aactgacgca gcgcctcaag ctgttgttct tcgtcatctt ccggcacgcg
      121 cagcaaatac tggcgcagtt catcacggta ggcatcggtc gccgtcggct gataaagggt
      181 atttggatcg agcaattcat ccagcaataa tggataacgc gctagctggc tggcaatcat
      241 cggcgatgcg gcacacagag aaatcaaatg tttaagcgcc gcagggaatt cactgagcaa
      301 ttctaaatag gttgtgcggg taacaatccc caccagcaag gcggtaatgc gcgacagcgt
      361 gacagcagcg tcttcacgcg cacagacatc acttagcaga tgtggcatca gatggtcgag
      421 cacctgacgt cctcgcggcc cgatagtacg cttatccagc tctttgcgga aatcagcaat
      481 cagcgttagg acctgtttgc gatcatcctc gctaagatgc gccagcactg gcgtagtgtc
      541 atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg actcttcctg
      601 agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat tggtcatatg
      661 tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc acgccagccg
      721 cgcacggtta agctcatcag aaggaagcgt ttgggtttgt tcgtcgttaa tgctttgcag
      781 caggttttcc agacgccgca ggaacagata tgccactcgc aattgttcag catcgttttc
      841 agaaagcaaa tgcagcgcgg caatggcgct gagcgttggc agtaaagagc gcgactgcag
      901 caacggttcg cgcccaccgc gaatgagctg gaacacctga acgataaatt caatttcgcg
      961 aatgccgcct gcgccgagtt tgatattgtc ggtcaaacca cgtcgacgca cttcacgggc
     1021 aatcatccct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt aacgacggaa
     1081 aacaaacggg cgcagcatcg cacgcaactc gttagcatag acgccttcgc tatcgcccat
     1141 aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgt ccctgctcct ggtaataatc
     1201 ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac gcagtcgcat
     1261 atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa tcagccgctg
     1321 ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct gcgtacaacc
     1381 atgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt ccccgccgcc
     1441 cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat tgcacggcgt
     1501 tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa tcagcgtctc
     1561 cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca gcgtttgcgc
     1621 ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca ttaacccggc
     1681 ttcgtcgctc acattactga gcgcctcctg caaccacacg ctgtaatgtt gccattcgtc
     1741 agcctgtggc ggttggcttt ccagttccgt cagccactct ggatgcgcaa tcacgctgtc
     1801 ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcactaagtg attcctcggc
     1861 taaaagctct ggcagccgct caacaacggt ctgccagtac tgctgtaacg gtgaagagag
     1921 cggcttcata agcgatttta tccttgcctg attaacgttt tccactgtgc aaccagaacg
     1981 gttcctgatt gtttgcctca ttacggaaat gttcaatttc aatgcgttgc ccggtcgcaa
     2041 tagcgtgacg cagcccctgc cagttctcca gccaggcttg cgcgacgaca ggatcatagt
     2101 aacccgccag caacagtatt gagtcaatat cacgcatcag gcgtggcaac tggtcacggt
     2161 agcgatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg cgggaaagat
     2221 ggatatcggc aaagcgtttg aaggagtcgc taattttgcc ctgcgctttg gcatctaaaa
     2281 acggctgcca tgctttgctt accagccatt cggtcaacgc cagcttcgcc attgccgttt
     2341 cggtagagta gaccgccgtc acggcagaaa ccgcagaagc gatggtcgct tcgcattgag
     2401 tcagcaaatc acgtaagtga gtactcgctt tacgcggcac aatgccaccg aacagcatca
     2461 gtgtatgacg gactaggcta atggctgcca gcacctgctc tttcgccgca tcgttgccgc
     2521 gtacccacag ttcttcatga tactgccatt gcgctaacac cagctcgagc gccgcttcca
     2581 gcccctgttc cacatcggct tttgccgcaa catgcaaaat ggtggtcggt ttgatttcac
     2641 gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg ctgccctggc
     2701 gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc gtgtcgccgc
     2761 taagcagctc cagttccagc tcgcagatag gctcagcaaa ttcacccgct ttcacttccc
     2821 cctggtcgag agcgatttca attcggctgc cattgatctc tactagccat ttttcgcgat
     2881 aaaaatcggt gctaaacagc gactgcacgc gggaagcgag atcggcaggc aattcgccgt
     2941 tcggccagat ttccgtcggt aactgcgcca ggtcgagcgt cggttcgctc aacgccacgt
     3001 tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc atggtcatct
     3061 catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc agccagttat
     3121 ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg ccgcccagcg
     3181 tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac tttaattcga
     3241 tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat gaacggcgaa
     3301 cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca tgcaacgggc
     3361 aatttgacgg gcgtaaaagt ttgaagcagt ggcaaggcct gacacagagg attccgattg
     3421 atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact ttccatgaca
     3481 ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc gcacttagcg
     3541 cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg aatacctggg
     3601 tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc ggcgaggaag
     3661 tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac agctctggcc
     3721 gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc tcccgtgtgc
     3781 cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc gataacacct
     3841 ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc gtgatcaacg
     3901 ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag aaaaaggtcg
     3961 atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa tggtttatgt
     4021 atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg cacctgatcc
     4081 caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt gcactgtctt
     4141 attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat ctggtcggtg
     4201 gtgctgttcg ggatgcattg ttaggactat cggtcaaaga cagagattgg gtggtggtcg
     4261 gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc gattttcctg
     4321 tgtttctgca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa cggaaatccg
     4381 gttccggtta caccggtttt acttgctatg ccgcaccgga tgtcacgctg gaagatgatc
     4441 ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc gagattatcg
     4501 acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt tcccccgctt
     4561 ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt tatgcccacc
     4621 tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc catgcgggtg
     4681 aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc ctcaccaccc
     4741 gcaatcccca ggtgttcttc caggtactgc gcgattgcgg cgcacttcgc gttttattcc
     4801 cggaaattga cgcactgttt ggcgttccgg cacctgccag gtggcatccg gaaatcgaca
     4861 cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg caggttgatg
     4921 tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca gaactttggc
     4981 cgcgtcatca tgggcatggc ccggcgggtg ttaagttagt ggaacaacta tgccagcgtc
     5041 tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt cacgatctca
     5101 tccgcacctt cccaatgctg aacccaaaaa ccatcgtcaa attatttgat tccatcgatg
     5161 cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct gacgtgcgcg
     5221 gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc gaaggctggg
     5281 aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa ggcgtggaga
     5341 ttcgcgagga gctgacccga cggcggattg cggcggtagc cagctggaag gaacaacgtt
     5401 gcccaaagcc tgactgagta taaaactaaa cgttagcacg aaccgtcccc tcgccccttc
     5461 ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc acccccaccc
     5521 cggccctctc cctacagagc gagggagaaa acccggcctg ctcccgaacc ttttctcaat
     5581 caaaagaaca cgacatacac cgcagccgcc acaataaagc gataaatggc gaacgggata
     5641 aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc caccacaaaa
     5701 gcggtgataa acccaacggc aaacatcggg atatcgccgg ttgtcaggaa gccccagctt
     5761 ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag gaacgagaac
     5821 tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt cgccccggaa
     5881 cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa cgcctgacga
     5941 taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca ttcggcggca
     6001 atcagcaaca aaccgccaac gaccagcgca tacatcacat ttatcgggtt aaacaatgac
     6061 ttaatcgtgt cgtggaataa tagccccagt accaccgccg gaatcatccc cagcaaaatg
     6121 tggatcagcg ttaaacgacc tttgctttca ccttcgtgct gcaacgggcg gccaaagtgg
     6181 atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag aattgatcct
     6241 aactggatca caacttcaaa ggttttcgcc gtgtcgccct caaaccccaa caagtgaccg
     6301 acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc ttcgaccaca
     6361 cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc cctaaattat
     6421 taaaatgtac cgcttgtccg aactactgca gtatgaccag gttataaccg tttggtttaa
     6481 cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat tacgccaaca
     6541 ttcgccgccc gcgccactgc gcctggcttg ctgagtttga tacgcaccca cggcgagttg
     6601 aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag cgcaaaacgc
     6661 gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag gcaatccgcc
     6721 acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc gaacactaac
     6781 ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac cgaaagttgc
     6841 tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga taccacttcc
     6901 ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca aaacggaaca
     6961 gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg gctccatttc
     7021 cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca gcggctccgg
     7081 caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg tagcagtact
     7141 gattttcgac gttctgaaag gaatgttgcc tgtctggggc gcgtatgaat taggtgtcag
     7201 ccccttctgg ctaggcttaa ttgccatcgc cgcctgtctt ggacacatct ggcccgtttt
     7261 cttcggtttt aaaggaggaa aaggcgttgc taccgctttt ggagcaatcg caccaattag
     7321 ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat tgagcggata
     7381 ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct ggtggtttaa
     7441 gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc gtcatcatga
     7501 caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat tcaaaagaaa
     7561 gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag ccttcatcgg
     7621 gcttgcccag ccgttgctgg caccatgccg ccagaaattc cgcgcagaca cgaatttcat
     7681 gctgcgatag agcagctccc ggtaaacagc ccagatattg gcgctttgtg catactccgg
     7741 caatacttgc accagtttgc cactctccag aaacggcaac acatcccact cggaacgcag
     7801 cataatccct ttgccctcca gcgcccattg caacactatc tcgccgctat tggaggaaag
     7861 atgcccgcta accttcaccg attttttctc ctgaccgttc cccaactccc atattccgtg
     7921 ggtcatatcg cgctctttgg tcaccagaca gtcatgacga cttaattcct gtaatgattg
     7981 tggctgcgga tatttttgca gatactcagg tgctgcgcat aatattcttt tatttttcgt
     8041 taacagatgc gcaatataat aatcaggaat ttcgtcatta atacgaatat ccagatcaat
     8101 attatcctgc actaaatcaa tttgccgatc gaacaattca aaatgcacct gtaactcagg
     8161 ataattgcgc ataagttcgg taatggctgg cgcaatatgg ctgcgcccaa aaccaaaact
     8221 gcaaccaata cgaatcatcc cctccgggcg cgttttgatt tgcgtgacgt catcgaccag
     8281 tcgctgatat tgggtgagga tttccagcgc atgttcgtaa caacgttggc cactttcagt
     8341 cagcgccacg ccgcgagcgg agcggttcag cagcgttgtt gccagcgtat tttcaaggat
     8401 ttggatccgc ttggtgacaa atgcaggcgt ttgcccaagc gtcgccgctg cagcactaaa
     8461 actcccgctg tggacgattt ctaccagtac ctgcaagtct ttggctaaag gccagctatt
     8521 cagcatcgtt aattatccgc agttgtgata agcgcagtgt atttcgcaaa acattgccat
     8581 tccctgctct ggcgcaatat tcattcttat tttcgcgttg attcacgaac tgttaattac
     8641 atattcacgg tggcaaaaaa tataaaacca catttttagt gttagtttgt ggcggtgaat
     8701 ttttccagac aaatacaaaa actggagttg ccatgatgag cgaaagtaat aagcaacagg
     8761 c
//
LOCUS       DAJVRL010000137         8694 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVRL010000137 DAJVRL010000000
VERSION     DAJVRL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.137"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..104)
                     /locus_tag="OQN02_003512"
     CDS             complement(<1..104)
                     /locus_tag="OQN02_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCS1644175.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="OQN02_003513"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="OQN02_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS1644176.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1353..2003
                     /gene="csgD"
                     /locus_tag="OQN02_003514"
     CDS             1353..2003
                     /gene="csgD"
                     /locus_tag="OQN02_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1644177.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2008..2397
                     /gene="csgE"
                     /locus_tag="OQN02_003515"
     CDS             2008..2397
                     /gene="csgE"
                     /locus_tag="OQN02_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS1644178.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2422..2838
                     /gene="csgF"
                     /locus_tag="OQN02_003516"
     CDS             2422..2838
                     /gene="csgF"
                     /locus_tag="OQN02_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS1644179.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2865..3571
                     /locus_tag="OQN02_003517"
                     /pseudo
     CDS             2865..3571
                     /locus_tag="OQN02_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3704..4195)
                     /locus_tag="OQN02_003518"
     CDS             complement(3704..4195)
                     /locus_tag="OQN02_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1644180.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="OQN02_003519"
     CDS             complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="OQN02_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1644181.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="OQN02_003520"
     CDS             complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="OQN02_003520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1644182.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="OQN02_003521"
                     /pseudo
     CDS             complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="OQN02_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6840..6927
                     /locus_tag="OQN02_003522"
     tRNA            6840..6927
                     /locus_tag="OQN02_003522"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6874..6876,aa:Ser,seq:gga)
     gene            complement(7120..8106)
                     /locus_tag="OQN02_003523"
     CDS             complement(7120..8106)
                     /locus_tag="OQN02_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644183.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(8103..8631)
                     /locus_tag="OQN02_003524"
                     /pseudo
     CDS             complement(8103..8631)
                     /locus_tag="OQN02_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2476 a         2088 c         1857 g         2273 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tatttttata
     1021 tgtattatta gtaagttagc accatttgta tgatttttta aaattgtgca ataaaaacca
     1081 aatgtacaac ttttctatca tttctaaact taataaaacc ttaatgttaa cattttaata
     1141 taatgagtta cattttgtta catgtttaac acttgattta agatttgtaa tggctagatt
     1201 gaaatcagat gtaatccatt agttttatat tttacccatt tagggctgat ttattactac
     1261 acacagcagt gcaacatctg tcagtacttc tggtgcttct attttagagg cagctgtcag
     1321 gtgtgcgatc aataaaaaag tggggtttca tcatgtttaa tgaagtccat agtattcatg
     1381 gtcatacatt attgttgatc actaaacctt ctttgcaggc gacagctctc ttgcagcacc
     1441 ttaaacaatc gctagcaatt acaggaaaat tacataatat tcaacgttct ctggacgata
     1501 tctcttcagg ctctattatt cttctggata tgatggaagc ggataaaaag cttatccatt
     1561 attggcagga tactttgagc aggaaaaaca acaatatcaa aatattgttg ctaaatacgc
     1621 ctgaagatta cccgtaccgc gacattgaaa actggcctca tatcaacggc gttttttatg
     1681 ccatggagga tcaagaacgt gttgtcaatg ggttgcaagg cgtcctgcgt ggcgaatgct
     1741 actttacgca aaagcttgcc agttacctga ttacacattc tggtaactat cgttataaca
     1801 gcacggaatc agccctcctt actcatcggg aaaaagagat cctgaataag ctgcgtatcg
     1861 gcgcgtctaa taacgagatc gctcgttcgt tgttcatcag cgaaaatacg gttaaaacgc
     1921 atctttataa tcttttcaag aagatagccg taaaaaaccg gacacaagcg gtttcctggg
     1981 caaacgataa cctcaggcga taaagccatg aaacgttatt tacgctggat tgtggcggca
     2041 gaatttctgt tcgccgcagg gaatcttcac gccgttgagg tagaagtccc gggattgtta
     2101 actgaccata ctgtttcatc tattggccat gatttttacc gagcctttag tgataaatgg
     2161 gaaagtgact atacgggtaa cttaacgatt aatgaaaggc ccagtgcacg atggggaagc
     2221 tggatcacaa taacggtcaa tcaggatgtt attttccaga cttttttatt cccgttgaaa
     2281 agagacttcg agaaaactgt cgtctttgca ctgattcaaa ctgaagaagc actaaatcgt
     2341 cgccagataa atcaggcgtt attaagtacg gacgatttgg cgcatgatga attctaaata
     2401 aaaaattgtt cggaggctgc aatgcgtgtc aaacatgcag tagttctact catgcttatt
     2461 tcgccattaa gttgggctgg aaccatgact ttccagttcc gtaatccaaa ctttggtggt
     2521 aacccaaata atggcgcttt tttattaaat agcgctcagg cccaaaactc ttataaagat
     2581 ccgagctata acgatgactt tggtattgaa acaccctcag cgttagataa ctttactcag
     2641 gccatccagt cacaaatttt aggtgggcta ctgtcgaata ttaataccgg taaaccgggc
     2701 cgcatggtga ccaacgatta tattgttgat attgctaacc gcgatggtca attgcagttg
     2761 aacgtgacag atcgtaaaac cggacaaacc tcgaccatcc aggtttcggg tttacaaaat
     2821 aactcaaccg atttttaagc cccagcttca taaggaaaat aatcatgcag cgcttatttc
     2881 ttttggttgc cgtcatgtta ctgagcggat gcttaaccgc cccgcctaaa gaagccgcca
     2941 gaccgacatt aatgcctcgt gctcagagct acaaagattt gacccatctg ccagcgccga
     3001 cgggtaagat ctttgtttcg gtatacaaca ttcaggacga aaccgggcaa tttaaaccct
     3061 acccggcaag taacttctcc actgctgttc cgcaaagcgc cagggcttac aaaacctgct
     3121 taacgagcgc aaggttattc gtgcggcaca agaaaacggc acggttgcca ttaataaccg
     3181 aatcccgctg caatctttaa cggcggcaaa tattatggtt gaaggttcga ttatcggtta
     3241 tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat tttgggatcg gtgcagacac
     3301 gcaataccag ctcgatcaga ttgccgtgaa cctgcgtgtc gtcaatgtga gtaccggtga
     3361 gatcctctcc tcggtgaaca ccagtaagac gatactttcc tatgaagttc aggccggggt
     3421 tttccgcttt attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcaaacga
     3481 acccgttatg ctgtgtctga tgtcggctat cgaaacaggg gtcattttcc tgattaatga
     3541 tggtatcgac cgtggtctgt gggatttgta aaataaagca gaacggcaga atgacattct
     3601 ggtgaaatac cgccatatgt cggttccacc ggaatcctga cagataaaaa tgccggaatt
     3661 aataatccgg catttactgt ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga
     3721 tttcctgctc acggtgagcc gtctttgcac tacgcgccgc cagccacagg ccgctgtttt
     3781 tcattgcgta accaaatacc agcccaagcg ccagcgaagg taacaccagt ttccagtcac
     3841 cctgcccggc aaacgtcgcg catgcgccta taaacgtccc cggaacaaat gaaagcagca
     3901 gctgtttggc ctgaatacac atcagaaacg cgacaatacc ggttatgaca taaccgagaa
     3961 tttccagatg tggtgccagt gcactaccgt aaataatgac catcgcccac accacgccac
     4021 taagcagggt tgcagcggag atcgccagcc ctttcagccc accttgcggg caagcaaagt
     4081 aggccgtaca gcccaggaag cccgcccagc ttagtaagcc cagggaaaca gccacccatc
     4141 cccagatacc ggagagaatg cctgttgtga ttgcaataga gagaagtatg ttcatgccgc
     4201 gcatgatagc agaaaacgcg gccatgaatg agatcgagca cacattttat gcaacaacaa
     4261 attatctgtt gcagttatga gtgacgatga tcacatttac tcttcagaat cttcttccag
     4321 ctcgtcccac attgcactaa tggcatcacg ggttagcggt gccatggtgc gccagaaagg
     4381 cgtggttgca tgggcctcca ctttgccgag gaacgcacca caccagggta acagatactc
     4441 actgaacagt gtttccagtg cttcgctctc atcttccgtt gactgatctt ccagccagga
     4501 agccgcaagc agcaatgtgc cgatgtgatc ggctggcgta tccgccagtg gcatccctcg
     4561 ttcggaaaga aaagcgcgca cttccgcttc cgttgcgtcc tcaacccatg cgctacgata
     4621 tggcggcacg gcacattcat cgccgataaa caacgcattg taatcggcag agacttgcgt
     4681 catatcacag cttttctgta aacgcgtcag taactcatcc tgctccagtg gccagttcgc
     4741 agccagtttc ccctcacgaa tcagagtaaa cagcggcacc agtaaaggat cttgcggttg
     4801 gcggtaatag agcgaaccca gcacacgaca gaggatagaa aactcgttca ttaatatatt
     4861 tccattacta gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc gcgagattca
     4921 aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc gctctagcgg aaaatctacc
     4981 gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg taaacgcggt gtgagaatcc
     5041 gagcctaatg ccacccaacc tccagcatca cgtaccgctg cagctaccgc acgacagttg
     5101 tcttcactgc ccttacgtga gtgtaaaaat gaggaattat tgatttccag cgccacctga
     5161 tgtttcgcgg ctgcttcagc aacggcttta acatctattt catatttcgg atttccggga
     5221 tggcttatta tatgcacatt gccgctggcg atagtggaga tcatcgcttg tgtattggtc
     5281 gctttgtcat gtggcgcaaa aaccggctca tgaaaaccgg caataatcag atccagcgag
     5341 tcaaacattt taccgctgca gtcaatttca ccatcaacat ttttaatgtt agcttcaatg
     5401 ccgcgcagga tccctacccc atcaaccact cgcggccaga tacgcatgtt aatgaagtgc
     5461 cagtgatgcg gcgcatcttc catatccggg ccatgatcgg tgatcgcaaa aagtttaatg
     5521 cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg tgtgctggca
     5581 acggtatgca tatgaaggtc gacgggatac ataactctct cctgtattca attcccgcca
     5641 aggatagcag gaatcctgat gctttattag tagccacgtg cgcggtcgac ttgcccgcag
     5701 accctctccc ctttttcgag ctgggcaata gtgcgagaaa tgtactccac agcttcagcg
     5761 ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga
     5821 ctttcaggcg gtaagggttc acgattaaaa acatccagca ttgcgccttt aactttcccg
     5881 ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa caccacgcgc caggttgagg
     5941 agatacgcgc catcaggtaa tttttcgagt aattgttgat taataatgcc gacggtttca
     6001 ggggtattcg gtaacaaatt aatcaatacc cgacattggc tcagaaatgc agacagttct
     6061 tcccgtccgg caaagctttg cacgccaggc cacgatttac gggttcgact ccagcaacgc
     6121 agcggaaagc gctaggtttg cagactctga gcaactttac agcccagtac gcctgcgccc
     6181 aaaatgccga tggtaaaatc ttcccgatga tattcaggca gcggttgcca atgcgaacta
     6241 ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg actgacagca
     6301 tattcctgca tttgctcgcc cataccggta tcttccaggc gaaaaagtgg aacagaaggg
     6361 ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt
     6421 gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa caggaggatg ccagactaaa
     6481 acataatcag cagagtcatt atctccgctt ttccatgccc tgactcttgc ctgaggaata
     6541 gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata aaagatgata
     6601 tccatactga ctcccgaaaa gcgttgtgcg aaatttattc gcacttatcg ttatgatcta
     6661 caaaggccac cagcataaca aatccgtggt cggtggcaaa aaaagcagat ttcgcttatt
     6721 aaaaccacac attgattgaa tgtttgaata aacgcgcgat tttttcaaaa agtttgttga
     6781 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     6841 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     6901 ggggttcgaa tccccccctc accgccatat ttaaagaaga gcgcgtacga aagtacgggc
     6961 ttttttttcg tatattgtac acaccgggtg cccccgccca cgtctgttag ggcgagggga
     7021 aattgtgcac atcaactgtc tggttactca gtcaacaatc aaaatgcgta agatagtaca
     7081 gaacatatat gcgttatctg gtcacttcct gtaacttcct cacttattaa aatgccccca
     7141 acggaaatac atgtcacatg ccatcataaa atcgttatgc cagaggaatc gacaatggaa
     7201 aaaatagtaa attaagcacg gtacatagca gagtaaaaaa taggcttcaa accaaatccg
     7261 ccattccaca agcgttgcca ataccgacac cagcgtaatc cacatcattt caataacccg
     7321 caatatgaat aaccgaagcc aaagcggctt ggcaaaaaag gtcgacaacg tattttcact
     7381 ggagaaaaac agatcgattt cctgcaaaaa aaacctgccc agaaaagcgg gccgcttgga
     7441 gaagcgcaga aaaaacagat taatcgccaa cactacgcca caaagaatga gcaatgcaat
     7501 tatcgattta aacgttaacc actgcccctc tgtcacaaaa gaatcactgt tacgcaacgg
     7561 gctgatagta agcaggttaa caaccgttga gatgcacacc gaaagcagca catagcgagt
     7621 cagactttta tattttactt ttttcgctac tgaaatagcg acctgcttga cataactggt
     7681 gcggatcatc cgaaacagca gaaagatgac caacggccca agtaacgaaa tcaggaacag
     7741 cgcatataac gagggtagaa gctggtatag cccccaactc attgcactca acatgattaa
     7801 cgacagaaat cctgccacca gaggttgtgt aagaaataat tctattttgt ggttctgctt
     7861 tttcttaaac cacgaaaaat cgatattctt cgtgacgcat ttataagccc agtacgcctt
     7921 gagatcaaaa ataaaattat acagcaccag ggccagaatc ccactaacta caccaatatc
     7981 ctctgatgga aaagtaaacc ctgcattttt ccaataaaag cctatcagaa taagtccaat
     8041 agcataaatg tatatcagat ttcgtttatc ctgaaagcga ataaaagtta aataatcagg
     8101 aatcatttga atacctttgc atcactaaat gctttacgac aatgcataac tcgttgctga
     8161 tccacgccag caaaatcatt taacatattc cccaaatagg gcttattact tatttcaagg
     8221 aaagctaccg gggttggcat attcagaata tgtatcgaga gattcagtcc atttcaccgt
     8281 atgagttaag tgtaatgaga gattttcttt gataattgac ggagcgacct cgctttttgc
     8341 tgttacgttc atcagaacct gatgctctgg cgaagcgata tccagtccgg caagataatc
     8401 gcgcattgcc tgaacgccgt cttccatcaa acgcgtgtgc caggcaccgc tcacaccgag
     8461 tttaaccggt tcgtatccag cggccatcag cagcgtggta aattcattca acgcagcctg
     8521 cgttccccca ataacctgct ggcgcggcgt gttatcacag ctaatatcca gcgcaatgcc
     8581 tgattccgta atcatcgtct gcagttgttc gcggttgatg cctttaaccg cctgatctta
     8641 cccagcaata gtggacacgc ggctaagtga gtaaactctc agtcagaggt gact
//
LOCUS       DAJVRL010000138         8412 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVRL010000138 DAJVRL010000000
VERSION     DAJVRL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.138"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OQN02_003525"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OQN02_003525"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1644184.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OQN02_003526"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OQN02_003526"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCS1644185.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OQN02_003527"
     CDS             3115..3399
                     /locus_tag="OQN02_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1644186.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OQN02_003528"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OQN02_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1644187.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OQN02_003529"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OQN02_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1644188.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OQN02_003530"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OQN02_003530"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1644189.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2019 a         2253 c         2139 g         2001 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gc
//
LOCUS       DAJVRL010000139         8395 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVRL010000139 DAJVRL010000000
VERSION     DAJVRL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.139"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            3..816
                     /gene="ttdA"
                     /locus_tag="OQN02_003531"
                     /pseudo
     CDS             3..816
                     /gene="ttdA"
                     /locus_tag="OQN02_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            813..1418
                     /gene="ttdB"
                     /locus_tag="OQN02_003532"
     CDS             813..1418
                     /gene="ttdB"
                     /locus_tag="OQN02_003532"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1644190.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1466..2929
                     /gene="ttdT"
                     /locus_tag="OQN02_003533"
     CDS             1466..2929
                     /gene="ttdT"
                     /locus_tag="OQN02_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1644191.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2972..3985)
                     /gene="tsaD"
                     /locus_tag="OQN02_003534"
     CDS             complement(2972..3985)
                     /gene="tsaD"
                     /locus_tag="OQN02_003534"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1644192.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4223..4438
                     /gene="rpsU"
                     /locus_tag="OQN02_003535"
     CDS             4223..4438
                     /gene="rpsU"
                     /locus_tag="OQN02_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1644193.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4549..6294
                     /gene="dnaG"
                     /locus_tag="OQN02_003536"
     CDS             4549..6294
                     /gene="dnaG"
                     /locus_tag="OQN02_003536"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1644194.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6489..8330
                     /gene="rpoD"
                     /locus_tag="OQN02_003537"
     CDS             6489..8330
                     /gene="rpoD"
                     /locus_tag="OQN02_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1644195.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2253 c         2252 g         1863 t
ORIGIN      
        1 cagtgaataa gttgacagag attgtcgcta actttaccgc catgatttct acccgaatgc
       61 ctgatgacgt ggtggataaa ctaaaacagc taaaggatgc cgaaacgtcg tcgatgggga
      121 aaattatcta ccatacgatg ttcgacaaca tgcaaaaagc gattgacctg aatcgtcctg
      181 cctgtcagga caccggggag attatgttct tcgttaaagt cggttcccgc ttcccactgc
      241 ttggcgagct gcaaagcata ctcaaacaag ccgtggaaga ggcaaccgtc aaagcgccac
      301 tacgtcacaa tgcggtagaa atttttgacg aagtaaacac cggcaaaaat accggtagcg
      361 gcgtaccgtg ggtcacctgg gacatcatcc ccgacaatga cgatgcggaa atcgaagttt
      421 acatggcagg cggcggctgc acgctacctg gccgctcgaa agtgttaatg ccgtcagaag
      481 gctacgaagg cgtggtgaaa ttcgtcttcg aaaatatctc caccctcgcc gtaaacgcct
      541 gtccaccggt actggtgggc gtgggcatcg ccacctcggt ggaaaccgcc gccgtactct
      601 cgcgtaaagc cattttgcgc ccgattggca gccgccatcc caatccaaaa gcggcagaac
      661 tggagctacg cctggaagaa ggactcaacc gtctggggat tggtccacaa gggctgaccg
      721 gcaacagttc agtgatgggc gcatcgtcgc ggcacgctgc tggttcatgc cgatctcacc
      781 tttgaaaatc tgtctcacac ccggagcgcg ttatgaaaaa gatcctgaca accccgatca
      841 aagctgaaga tctgcaagat attcgcgtcg gcgatgtgat ctacctgacc ggtacgctgg
      901 tgacctgccg cgacgtttgt caccgccgtt tgatcgaact gaaacgtccg atcccttacg
      961 atctcaacgg caaagcgatt ttccacgctg gccccatcgt gcgcaaaaac ggtgacaaat
     1021 gggagatggt ctccgtcggc ccgacaacca gtatgcgtat ggaaagtttt gaacgtgaat
     1081 ttattgagca gaccggcgta aaactggtgg ttggcaaagg tggtatgggg ccgctgaccg
     1141 aagaaggctg ccagaaattc aaggcgttac atgtgatttt cccagcaggc tgcgcggtgc
     1201 tggcggcaac ccaggtagaa gagattgaag aagtgcactg gacagaactc gggatgccgg
     1261 agtcactgtg ggtctgccgg gtcaaagagt tcggcccgct gattgtctct attgataccc
     1321 acggcaacaa cctgatagcc gaaaacaaaa agctgttcgc cgaacgccgc gatcccatcg
     1381 tggaagagat ctgcgagcac gtccattaca tcaaataacc ctcccggaga ggctcacccc
     1441 tctccttttt cgcaggcata acacgatgaa accttccact gaatggtggc gatatcttgc
     1501 gccgctggcg gtcatcgcca ttattgctct acttccggtt cccgcagggc tggagaacca
     1561 tacctggctc tactttgccg tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt
     1621 gccgggtgcc gtggtggcga tggtgggtat ctccatcatc gccatactct ctccctggct
     1681 gctgttcagc cccgaacagc tcgcccagcc aggctttaag ttcaccgcca aatccctctc
     1741 ctgggcagtt tctggttttt ctaattcggt tatctggctc attttcgccg cctttatgtt
     1801 tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa
     1861 gatggggcat cgcacgctgt ttctcggcta tgcggtgatg ttctccgagc tgatcctggc
     1921 acctgtaaca ccgtccaact cggcgcgtgg tgcgggtatt atctatccca tcatccgtaa
     1981 cctgccaccg ctctatcaat cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta
     2041 catcatgtgg atggggattg ttgccgactg cgtgaccagc gccattttcc tgacggcgat
     2101 ggcacctaac ttgctgttaa ttggactgat gaaaagcgca tctcacgcca cgctgagttg
     2161 gggcgactgg ttcctcggga tgttgccgct cagcatttta ctggttctgc tggttccctg
     2221 gctggcttac gtgctgtacc cgccggtact gaagtctggt gatcaggtgc cgcgctgggc
     2281 agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct
     2341 gatggtaggc gcgctggtgc tgtggatttt cggcggtgat tatatcgatg ccgcgatggt
     2401 cggttacagc gtggtggcgc tgatgctgct tctgcgcatt atcagttggg acgacattgt
     2461 cagtaataaa gcggcatgga acgttttctt ctggctggcc tcgcttatca ccctcgcgac
     2521 cggactcaac aataccggtt ttattagctg gtttggcaaa ctgttagcag gcagcttaag
     2581 tggttattcg ccgacgatgg tgatggtgac gttgattgtg gtgttttatc tactgcgcta
     2641 ctttttcgcc agcgccacgg cgtatacctg cgccctcgca ccgatgatga tcgccgccgc
     2701 gctggcgatg ccggaaatcc cgctgccggt gttctgcctg atggttggcg cggcaattgg
     2761 tctggggagc attcttacac catacgccac cggacccagt ccgatttact acggtagtgg
     2821 ttatctgcca acggcggatt actggcgact gggggcgatt tttgggctga tattcctcgt
     2881 gttgctggtg attaccggct tactgtggat gcccgtggtg ttgctttaag tgaggacggt
     2941 gccggaaatt gctctccggc acccaatagt tttacgcagc cggtaactcc gccagcggcc
     3001 agcgcggacg cacgctaaca ccgagatccg ccgtcgcgcc tgctttaaac cgcaccattc
     3061 cggcataggc gatcatcgcg ccgttatcag tacaaaattc cggacgcgcg tagaacactt
     3121 cgccgcggcg ttttttcatc atttcagcca gcttcgcccg taacgtgcgg ttagcactca
     3181 cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc caacgctcgc ttacacttaa
     3241 tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc gatatcagca cgcgtctggt
     3301 cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt tttcagacca gaaaagctga
     3361 aatccagccc cggacggtcg gtcatcggac gcgggaaaac aaagcgcccg gcagtaccct
     3421 gagccgccat tttcgacagt aacggtccgc caggataatc cagccccagc agcttcgcgg
     3481 ttttatcaaa cgcttcaccg gcggcatcat cgatagactc gccgagcagc tcgtactgac
     3541 caatgccagt cacgctgatt aactgcgtat gaccgccgga caccagcagc gcgacaaacg
     3601 gaaattccgg cgggttatct tccagcatcg gcgctaacag atgcccttcc atatggtgta
     3661 ccgggattgc cggaacgttc caggcaaacg ccagagaacg ccccacggtt gcgccaacca
     3721 gcagtgcgcc gactaatcca gggcctgcgg tataggccac agcatcaatg tcttttgccg
     3781 ttaaaccaga ctcctttagc gccgcctgga tcaacggtac ggttttacgc acatgatcgc
     3841 gggaggccag ttcaggcacg acgccgccgt agtcagcgtg caatttcacc tgactataca
     3901 attggttggc taacaaacct ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg
     3961 aagtttcaat acccagtaca cgcatgactt gttttacctc gctttattac cgcgcagtgt
     4021 aggaccaatg cgggttgatg taaaactttg ttcgcccctg gagaaagcct cgtgtatact
     4081 cctcaccctt ataaaagtcc ctttcaaaaa aggccgcggt gctttacaaa gcagcagcaa
     4141 ttgcagtaaa attccgcacc attttgaaat aagctggcgt tgatgccagc ggcaaaccga
     4201 attaatcaaa ggtgagaggc acatgccggt aattaaagta cgtgaaaacg agccgttcga
     4261 cgtagctctg cgtcgcttca agcgttcctg cgaaaaagca ggtgttctgg cggaagttcg
     4321 tcgtcgtgag ttctatgaaa aaccgactac cgaacgtaag cgcgctaaag cttctgcagt
     4381 gaaacgtcac gcgaagaaac tggctcgcga aaacgcacgc cgcactcgtc tgtactaatt
     4441 ccccgagagc gttgctctcc gatcagaccg agttgtagtt gtaaggccgt gcttccgaaa
     4501 ggaatgcgcg gcttattttc gtttatgaat tgctaaaaat cggggcctat ggctggacga
     4561 atcccacgcg tattcattaa tgatctgctg gcacgcactg acatcgtcga tctgatcgat
     4621 gcccgtgtga agctgaaaaa gcagggcaag aatttccacg cgtgttgtcc attccacaac
     4681 gagaaaaccc cgtcattcac cgttaacggt gagaaacagt tttaccactg ctttggatgt
     4741 ggcgcgcacg gcaacgcgat cgacttcctg atgaactacg acaagctcga gttcgtcgaa
     4801 acggtcgaag agctggcagc aatgcacaat cttgaagtgc catttgaagc aggcagcggc
     4861 cccagccaga tcgagcgcca tcagaggcaa acgctttatc agttgatgga cggtctgaat
     4921 acgttttacc aacaatcttt acaacaacct gttgccacgt ctgcgcgcca gtatctggaa
     4981 aaacgcggat taagccacga ggttatcgct cgctttgcga ttggttttgc gccccccggc
     5041 tgggacaacg tcctgaagcg gtttggcggc aatccagaaa atcgccagtc attgattgat
     5101 gcggggatgt tggtcactaa cgatcaggga cgcagttacg atcgtttccg cgaacgggtg
     5161 atgttcccca ttcgcgataa acgcggtcgg gtgattggtt ttggcgggcg cgtgctgggc
     5221 aacgataccc ccaaatacct gaactcgccg gaaacagaca ttttccataa aggccgccag
     5281 ctttacggtc tttatgaagc gcagcaggat aacgctgaac ccaatcgtct gcttgtggtc
     5341 gaaggctata tggacgtggt ggcgctggcg caatacggca ttaattacgc cgttgcgtcg
     5401 ttaggtacgt caaccaccgc cgatcacata caactgttgt tccgcgcgac caacaatgtc
     5461 atttgctgtt atgacggcga ccgtgcaggc cgcgatgccg catggcgagc gctggaaacg
     5521 gcgctgcctt acatgacaga cggccgtcag ctacgcttta tgtttttgcc ggatggcgaa
     5581 gaccctgaca cgctggtacg aaaagaaggt aaagaagcgt ttgaagcgcg gatggagcag
     5641 gcgatgccac tctccgcatt tctgtttaac agtctgatgc cgcaagttga tctgagtacc
     5701 cctgacgggc gcgcacgttt gagtacgctg gcactgccat tgatatcgca agtgccgggc
     5761 gaaacgctgc gaatatatct tcgtcaggaa ttaggcaaca aattaggcat acttgatgac
     5821 agccagcttg aacgattaat gccaaaagcg gcagagagcg gcgtttctcg ccctgttccg
     5881 cagctaaaac gcacgaccat gcgtatactt atagggttgc tggtgcaaaa tccagaatta
     5941 gcgacgttgg tcccgccgct tgagaatctg gatgaaaata agctccctgg acttggctta
     6001 ttcagagaac tggtcaacac ttgtctctcc cagccaggtc tgaccaccgg gcaactttta
     6061 gagcactatc gtggtacaaa taatgctgcc acccttgaaa aactgtcgat gtgggacgat
     6121 atagcagata agaatattgc tgagcaaacc ttcaccgact cactcaacca tatgtttgat
     6181 tcgctgcttg aactgcgcca ggaagagtta atcgctcgtg agcgcacgca tggtttaagc
     6241 aacgaagaac gcctggagct ctggacatta aaccaggagc tggcgaaaaa gtgatttaac
     6301 ggcttaagtg ccgaagagcg atcgggaagc ccccgacagc cgcactgaga ggcagcggca
     6361 aatatataag tacgccctcg taattatcgt tggcggtaaa caaccgttgg atttcagcgt
     6421 taacggctga aggacatcgg gtcaatcgcc caacaccaac ctcatgaaat aagtgtggat
     6481 accgtcttat ggagcaaaac ccgcagtcac agctgaaact tcttgtcacc cgtggtaagg
     6541 agcaaggcta tctgacctat gccgaggtca atgaccatct gccggaagat atcgtcgatt
     6601 cagatcagat cgaagacatc atccaaatga tcaacgacat gggcattcag gtgatggaag
     6661 aagcaccgga tgccgatgat ctgatgctgg ctgaaaacac cgcggacgaa gatgctgccg
     6721 aagccgccgc gcaggtgctt tccagcgtgg aatctgaaat cgggcgcacg actgacccgg
     6781 tacgcatgta catgcgtgaa atgggcaccg ttgaactgtt gacccgcgaa ggcgaaattg
     6841 acatcgctaa gcgtattgaa gacgggatca accaggttca atgctccgtt gctgaatatc
     6901 cggaagcgat cacctatctg ctggaacagt acgatcgtgt tgaagcagaa gaagcgcgtc
     6961 tgtccgatct gatcactggc tttgttgacc cgaacgcaga agaagatctg gcacctaccg
     7021 ccactcacgt cggttctgag ctttcccagg aagatctgga cgatgacgaa gatgaagacg
     7081 aagaagatgg cgatgacgac agcgccgatg atgacaacag catcgacccg gaactggctc
     7141 gcgaaaaatt tgcggaacta cgcgctcagt acgttgtaac gcgtgacacc atcaaagcga
     7201 aaggtcgcag tcacgctgcc gctcaggaag agatcctgaa actgtctgaa gtattcaaac
     7261 agttccgcct ggtgccgaag cagtttgact acctggtcaa cagcatgcgc gtcatgatgg
     7321 accgcgttcg tacgcaagaa cgtctgatca tgaagctctg cgttgagcag tgcaaaatgc
     7381 cgaagaaaaa cttcattacc ctgtttaccg gcaacgaaac cagcgatacc tggttcaacg
     7441 cggcaattgc gatgaacaag ccgtggtcgg aaaaactgca cgatgtctct gaagaagtgc
     7501 atcgcgccct gcaaaaactg cagcagattg aagaagaaac cggcctgacc atcgagcagg
     7561 ttaaagatat caaccgtcgt atgtccatcg gtgaagcgaa agcccgccgt gcgaagaaag
     7621 agatggttga agcgaactta cgtctggtta tttctatcgc caagaaatac accaaccgtg
     7681 gcttgcagtt ccttgacctg attcaggaag gtaacatcgg cctgatgaaa gcggttgata
     7741 aattcgaata ccgccgtggt tacaagttct ccacctacgc aacctggtgg atccgtcagg
     7801 cgatcacccg ttctatcgcg gatcaggcgc gcaccatccg tattccggtg catatgattg
     7861 agactatcaa caaactcaac cgtatttctc gccagatgct gcaagagatg ggccgtgaac
     7921 cgacgccgga agaactggct gaacgtatgc tgatgccgga agacaagatc cgcaaagtgc
     7981 tgaagatcgc caaagagcca atctccatgg aaacgccgat cggtgatgat gaagattcgc
     8041 atctggggga tttcatcgag gataccaccc tcgagctgcc gctggattct gcgaccaccg
     8101 aaagcctgcg tgcggcaacg cacgacgtgc tggctggcct gaccgcgcgt gaagcaaaag
     8161 ttctgcgtat gcgtttcggt atcgatatga acaccgacca cacgctggaa gaagtgggta
     8221 aacagttcga cgttacccgc gaacgtatcc gtcagatcga agcgaaggcg ctgcgcaaac
     8281 tgcgtcaccc gagccgttct gaagtgttgc gtagcttcct ggacgattaa tcggtaggcc
     8341 ggatcaggcg ttacgccgca cccggcacta gtctctctgc acaaacgcca ccttt
//
LOCUS       DAJVRL010000140         8387 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVRL010000140 DAJVRL010000000
VERSION     DAJVRL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.140"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OQN02_003538"
     CDS             complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OQN02_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1644196.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1968..3197)
                     /gene="hemX"
                     /locus_tag="OQN02_003539"
     CDS             complement(1968..3197)
                     /gene="hemX"
                     /locus_tag="OQN02_003539"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1644197.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(3219..3959)
                     /gene="hemD"
                     /locus_tag="OQN02_003540"
     CDS             complement(3219..3959)
                     /gene="hemD"
                     /locus_tag="OQN02_003540"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1644198.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3956..4897)
                     /gene="hemC"
                     /locus_tag="OQN02_003541"
     CDS             complement(3956..4897)
                     /gene="hemC"
                     /locus_tag="OQN02_003541"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1644199.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5284..7830
                     /gene="cyaA"
                     /locus_tag="OQN02_003542"
     CDS             5284..7830
                     /gene="cyaA"
                     /locus_tag="OQN02_003542"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1644200.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7870..8190)
                     /gene="cyaY"
                     /locus_tag="OQN02_003543"
     CDS             complement(7870..8190)
                     /gene="cyaY"
                     /locus_tag="OQN02_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1644201.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8238..>8387)
                     /locus_tag="OQN02_003544"
     CDS             complement(8238..>8387)
                     /locus_tag="OQN02_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1644202.1"
                     /translation="EVRITPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEV
                     QTLKP"
BASE COUNT         1894 a         2185 c         2171 g         2137 t
ORIGIN      
        1 agttgacggg gtatctgctg cgctggccat gagaggaaga gcggcggcga cagcaacaac
       61 aagacgtttg ggtgaaaacg aaaattccat gcaaaatgct ccggtttcat gtcatcaaaa
      121 tgatgacgta attaagcatt gataattgag atccctctcc ctgacaggat gattacataa
      181 ataatagtga caaaaataaa ttatttattt atccagaaaa tgaattggaa aatcaggaga
      241 gcgttttcaa tcctacctct ggcgcagttg atatgtaagg caggtttatt atatcgcgtt
      301 gattattgat gctgttttta gttttaacgg caattaatat atgtgttatt aattgaatga
      361 attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat tcactcagga
      421 agttattact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      481 gttatttccc gattctctgt ggcataataa acgagtagat gctcattcca tctcttatgt
      541 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      601 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      661 cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      721 tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg agaaggaact actgtggcgg
      781 gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      841 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      901 cagcgccgcg cggaaggcga gcgatgcttc ctgccattca ccgtgcttca tcagtgactg
      961 acccaatgtg ctccacaaca gcgggcgatc accgacgttt ttgatttgct ggcgtagcac
     1021 tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca gtaggcgatc
     1081 gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat gatcgtcaca
     1141 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
     1201 ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg cctgatccat
     1261 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
     1321 tttcgccatt gatgggatga tatccagcag agaactccat gcccctgtgc ggatatacgc
     1381 ctgttccgcc agacgtaata cttcaggatg gcgtggcgta acttccagca gcttatccac
     1441 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1501 taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg
     1561 tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1621 ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat caccttccgc
     1681 cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg cgacgcttac gtccgacaaa
     1741 ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgc agtagccact caatggcaaa
     1801 cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg tttcgatatt
     1861 gtagttgtcg gtctggatca gcacataacc ctgatgaccg gcaatcatcg ggccaaccac
     1921 tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta ttctccttgc
     1981 ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc aggtgcaggt
     2041 gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg ttgtccccgc tgccggttgt
     2101 gccagcaggt tacgcacgcg agtctgcatc agtttttcca gcatcgcctg gctttgcagg
     2161 gtttccggaa gatccatcga gatattttgc tggcttaact ggtccacctc gtcgaggaac
     2221 gctttggtgg tggcatcatc agtatcgtag taagcacgta cccaggtgga gacgttctcc
     2281 agcgcctggc gataagtctc ttcctgatga cgcggtacag cttgtgctgc gaccagcagg
     2341 cgagagcgaa tattttcgcg cagatagata tcctgatttg gcgctaacag cggtacggcg
     2401 gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa agttctgcca gcttttttgc
     2461 agattgatac gccattcgct gatggaactg gaaagctctt caccgtctga atccatcggc
     2521 gaaccatcgc tgtcattatc ggccagacgc aggttatcta cctgatttga aagctgatta
     2581 agcttaagga tgatgccgtc ataatccacc tgcgatactg cagaaaggct ggcgatatca
     2641 tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat tcatatccgc caggctggcg
     2701 tctgcacttt tcagcaacgc tgcagaggtc gtgacgtcct gatcgctcca cagcttccgt
     2761 ccggcgagtt tcaccagaaa atcggcctga gccagcagcc aggttttagc atcgctgccg
     2821 gaaatggtgg cgatcttttg ttggacttca tccaactgtt ttgccagcgt ttcttgctga
     2881 cgattcgcct gcttaagctg cgcagcttgt tgcttaataa tgccttccag ctcggctttt
     2941 tggctctcct gggctttttg caatgccgtc agttggttag ccagggcatc gctggtggcg
     3001 gtctgattga cggcctgttg tttaccccag ccatacaaac cgacacccgc cgccagagca
     3061 atagcgatag ccaccgcgct gagaatcaat gcagtattgt tcttactctt tttttctgtt
     3121 gcgacaggtt gtgacgtggt gtccacggcc tccctggtct cttcaaccac ggcggaggtt
     3181 ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt attgtaatgc ccgtaaaagc
     3241 gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc agcccagttc ccgggcgagt
     3301 ttcgccaaac gctcactgac gaccaatagt cgacagtgta gtaaccagtg ctcacgatac
     3361 cattgtggga tcagcgacca gagttgctgc aacatttcac cgctggtaac aacgaccatc
     3421 gtcacctcgc gggattgcca gcgcatcgct tcttctgcac catcgtaatg gattgcgcat
     3481 cgttgataac attcacaaaa agtgacctca gcaccgcgca ccgtcagggt atccccaatt
     3541 agctcacgac cgccattgcc acgtaatatc agcgcacgtt tgcccgcaat attttgtaat
     3601 tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct gtgggtagag aatcttctgt
     3661 ccgcttacgg tatgtagcgc cagtgcggtg gtgcgtccaa tggcgaaata agtaggtagt
     3721 tggggccatt tacgatcttg ctgatgcagc tgtgattggg caaaagcaac cgcgtgttgc
     3781 gagagggcaa acaacagatc gctctccccc agcgctgcca gttgatcagc aagttgcggt
     3841 aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc aggccacctg ccccagtgtg
     3901 cgcagacggc tcactaactc ttctccagcg ggagacgggc gggtgaccag gatactcatg
     3961 ccggggcctc tccgttatag acttcagcga ggatctcgcg cgcgccgtta ttcagtagct
     4021 cttctgccag cgaaatcccc atttgttcgg catcttgcgg cgcaccgcgg cgttcaccgc
     4081 gaataatctg cgaaccgtcc ggcgcgccga ccagcgcaca tagccagatt tcgccatcaa
     4141 taagctcggc gtagctacca attggcacct gacatccgcc ttcgagacgg gtattcatgg
     4201 cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt cagcgcggca agcagctcgc
     4261 gagtgcgtgt atcatcaagg cggcattcaa taccgactgc accttgtcct accgccggaa
     4321 gagaaatctc gggtggtaac gcggcgcgaa tgcgggactc cagacctaaa cgttttagtc
     4381 cggctacggc aagaatgatg gcatcgtatt cgccgttatc tagtttgctc aggcgagtgc
     4441 cgacgttgcc gcgcagggag cggataatca gatccgggcg gcgttcagcc agttggcact
     4501 ggcgacgtaa actggacgtc ccgacgatac tgcctgctgg taacgcatcc agactgtcat
     4561 agttattgga cacaaaggca tcgcgaggat cttcacgctc gcaaatagtg accagcccca
     4621 gaccttgcgg gaattcaacc ggcacatctt tcattgaatg tacggcgata tcagcgcgat
     4681 tctccaggag cgcgacttcc agctctttta caaataagcc ttttccgcct actttcgcca
     4741 gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg taccagttca acgaccaggc
     4801 ccggatggct cgccatcaac ttgtctttga catagtgtgc ctgccagagt gcaagtgggc
     4861 tttggcgtgt ggcaattctt aaaacattgt ctaacatgct tgttaccgtc attatcatcc
     4921 gtggtccatc ctaacatcct tgccagagtg atgtcagtgt tgtggtgaaa cgtagacgcc
     4981 tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac aggcgtttca cgccgtcgta
     5041 ataaggaatt tacagagaat aaacggtgct acacttgtat gtagcgcatc tttctttacg
     5101 gtcaatcagc aaggtgttaa attgatcacg ttttagacca ttttttcgtc gtgaaactaa
     5161 aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca tacgaaatat cccgaatgcc
     5221 gcgtgttacc gttgatgttg gcggaatcac agtcatgacg ggtagcaaat caggcgatac
     5281 gtcttgtacc tctatattga gactctgaaa cagagactgg atgccataaa tcaattgcgt
     5341 gtggatcgcg cgcttgctgc tatggggcct gcattccaac aggtctacag tctactgccg
     5401 acattgttgc actatcacca tccgctaatg ccgggttacc ttgatggtaa cgttcccaaa
     5461 ggcatttgcc tttacacgcc tgatgaaact caacgccact acctgaacga gcttgaactg
     5521 tatcgtggaa tgtcagtaca ggatccaccg aaaggtgagc ttccaattac tggtgtatac
     5581 accatgggca gcacctcgtc cgtagggcaa agttgttcct ctgacctgga tatctgggtc
     5641 tgtcatcaat cctggctcga tagcgaagag cgccagttac tacaacgtaa atgcagcctg
     5701 ctggaaagct gggccgcctc gctgggtgtg gaagtcagct tcttcctgat tgatgaaaac
     5761 cgcttccgtc ataatgaaag cggcagcctg gggggcgaag attgtggctc cacccagcat
     5821 atactgctgc ttgacgaatt ttatcgtact gccgtgcgtc tcgccggtaa gcgtattctg
     5881 tggaatatgg tgccgtgcga cgaagaagag cattacgacg actatgtgat gacgctttac
     5941 gcgcagggcg tgctgacgcc aaatgaatgg ctggatctcg gtggcttaag ctcgctttct
     6001 gctgaagagt actttggtgc cagcctttgg cagctctaca agagtatcga ttccccatac
     6061 aaagcggtgc tgaaaacact gctgctggaa gcctattcct gggaataccc gaacccacgt
     6121 ctgctggcga aagatatcaa acagcgtttg cacgacggcg agattgtatc gtttggcctc
     6181 gatccatact gcatgatgct ggagcgtgtt actgaatacc tgacggcgat tgaagatttc
     6241 acccgtctgg atttagtacg tcgctgcttc tatttaaaag tgtgcgaaaa actcagccgt
     6301 gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc agttagtgag cgagtggggt
     6361 tgggacgaag ctcgtctggc aatgctcgat aaccgtgcta actggaagat tgatcaggtg
     6421 cgtgaggcgc acaacgagct gctcgacgcg atgatgcaga gctaccgtaa tctgatccgc
     6481 tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc aggatatcgg cgtactgacg
     6541 cgtaaactgt atgccgcgtt tgaagcattg ccaggtaaag tgacgctggt aaacccgcag
     6601 atttcacccg atctctcgga accgaatctg acctttattt atgtgccgcc gggccgggct
     6661 aaccgttcag gttggtatct gtataaccgc gcgccaaata ttgagtcgat catcagccat
     6721 cagccgctgg aatataaccg ttacctgaat aaactggtgg cgtgggcatg gtttaacggc
     6781 ctgctgacct cgcgcacccg tttgtatatt aaaggtaacg gcattgtcga tttgcctaag
     6841 ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc tgcgcttacc tgcaccgaca
     6901 ccgaaggcgc tctacagccc gtgtgagatt cgccatctgg cgattatcgt taacctggaa
     6961 tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt ttgatttccg taagctggat
     7021 gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta gcgttgacct gctgtaccgc
     7081 aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg agcaatcgat gatcgaagcc
     7141 ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac cgccagatag cgtggaagtc
     7201 ttctgttata gccagcatct gcgcggctta atccgtactc gcgtgcagca actggtttct
     7261 gagtgtattg aactgcgcct ttccagcacc cgccaggaaa ccgggcgttt caaggcgctg
     7321 cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc tgaatgtatc ggtacagaag
     7381 ctggagaacg ccatcgagtt ttatggcgct atttcgcata acaaactgca cggcttgtca
     7441 gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg tggacggctt tgccagcgaa
     7501 gggatcatcc agttcttttt cgaagaaacg caagacgaga atggctttaa tatctacatt
     7561 ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg aaggcagcaa agaggagctg
     7621 gtacgtgacg tcagtcgctt ctactcgtca tcgcacgacc gcttcaccta cggctcaagc
     7681 ttcatcaact tcaacctgcc gcagttctat cagattgtga aggttgatgg tcgcgaacag
     7741 gtgattccgt tccgaacaaa atcgattggc aacctgccgc ccgccaatca ggatcacgat
     7801 acgccgctat tacagcagta tttttcgtga tgagcgtgcc ggaaagcgag gcttatccgg
     7861 catgcaatct tagcggaaac tgactgtttc acccgcctgt tgcgtcgccg cctgttccag
     7921 caaatcccag aaggtttcgc cgctgcgatc gcaaatccat tcatcgcctt tcaggtcaaa
     7981 atggtagccg ccctgtttgg ttgccagcca cacctggtgc agcggctcct ggcggttaat
     8041 aatgattttg ctgccgtttt caaaggtaat ggtcagtacg ccgccgttga tttcgcagtc
     8101 gatatcgctg tcgccatccc tgtcgtccag acgctcttca atggtcagcc acagttgatc
     8161 agccaggcga tgaaattcac tgtcgttcat tgttgtatcc tgtttttaag tgatggcggc
     8221 agtatagcgg catggggtca gggcttcaaa gtttgcacct ctgctgctgc gttccggcat
     8281 gattcatccg tcaccggaat aatgatgtct ctgttttgaa cttcacgtac gttctcctct
     8341 ctgacacgag ttttcgccgg gcagggggag ggcgtaatcc ttacctc
//
LOCUS       DAJVRL010000141         8263 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVRL010000141 DAJVRL010000000
VERSION     DAJVRL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.141"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            68..316
                     /locus_tag="OQN02_003545"
     CDS             68..316
                     /locus_tag="OQN02_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCS1644203.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            381..749
                     /locus_tag="OQN02_003546"
     CDS             381..749
                     /locus_tag="OQN02_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1644204.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            843..1496
                     /gene="nfsB"
                     /locus_tag="OQN02_003547"
     CDS             843..1496
                     /gene="nfsB"
                     /locus_tag="OQN02_003547"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS1644205.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1604..2851
                     /gene="ybdG"
                     /locus_tag="OQN02_003548"
     CDS             1604..2851
                     /gene="ybdG"
                     /locus_tag="OQN02_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1644206.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="OQN02_003549"
     CDS             complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="OQN02_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1644207.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="OQN02_003550"
     CDS             complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="OQN02_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS1644208.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="OQN02_003551"
                     /pseudo
     CDS             complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="OQN02_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2016 a         2307 c         2013 g         1927 t
ORIGIN      
        1 aagccgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
       61 caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc
      121 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
      181 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
      241 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
      301 ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc ggcatacttt gcttattctc
      361 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctag
      421 ctcttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
      481 aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc
      541 tgaagtccga tccacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag
      601 ggtatcacat gaataaaaag cactggattt cggtgtattc cggcgaggaa atctccgaag
      661 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
      721 gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct atagcgcatt tttctcgctt
      781 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attacttaca catggagtct
      841 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca
      901 aaaaacttac cccggaacag gccgagcaga tcaaaacgct actgcaatac agcccatcca
      961 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
     1021 ttgccaaatc cgctgccggt aattatgtgt tcaacaaacg taaaatactt gatgcctcgc
     1081 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
     1141 accaggaaga tgccgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
     1201 gtcgcaagtt cttcgccgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
     1261 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg
     1321 acgcggtgcc catcgaaggt tttgacgccg caatcctcga tgcagaattt ggtctgaaag
     1381 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
     1441 acgctacgct gccgaaatct cgtctgccgc aaaacaccac cttaaccgaa gtgtaattct
     1501 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
     1561 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
     1621 ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
     1681 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
     1741 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
     1801 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
     1861 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
     1921 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
     1981 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
     2041 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
     2101 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc
     2161 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
     2221 ctggctggaa atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
     2281 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
     2341 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag
     2401 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
     2461 taaagcgcat ttgttaaagc cttatttaac cagccgccat caggaaatta atgagtggaa
     2521 tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc cgaatgacca atattggaac
     2581 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
     2641 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
     2701 tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
     2761 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat
     2821 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
     2881 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
     2941 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
     3001 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg cgatgcaaag atagttgccg
     3061 aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgccgcgcga
     3121 aaacgcagat gcgccaggca gatcataatc cagttcaaca gcaacgttgc taccaccagc
     3181 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa
     3241 gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct gacgcgagtc
     3301 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
     3361 accccgctgt tatacaccga cagcgatgcc actagaatga cgaagttcag cgcagaagct
     3421 accacattgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
     3481 tccacccacg gatagagtgc cagtaaaacc accagtgaac cgatataaaa cagcaggatg
     3541 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
     3601 gctgtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
     3661 atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg
     3721 gctttctcgc cgccgtaacc agaaaacagc agccacaggc caaagccgat cataccgatg
     3781 atcgccagca ctttaatcag cgcaaaccag aactcggttt cgccatataa gcgcacgttc
     3841 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
     3901 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
     3961 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
     4021 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccaagctgg
     4081 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
     4141 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
     4201 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac
     4261 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
     4321 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
     4381 acgaggtatc ctgctttaat tccgtacccg atgtcggtgc agccacatca gcttatacgc
     4441 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc
     4501 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     4561 ggcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     4621 caacgcctca tccagcttct gctcgctgaa tgtttgtgga ttattcaacg acggctcggc
     4681 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc
     4741 aagggcaata aagccggtac ccgtcgccac agaaagatga aagcccatcc accacagtaa
     4801 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     4861 ccgacggaat gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag
     4921 tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     4981 gcccggtttt aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac
     5041 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     5101 cagcatcgac ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg
     5161 cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc ggactatcgc gccagctttg
     5221 cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc caaccatcgc
     5281 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgtgc
     5341 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc gctcagcaag
     5401 cgcagaagct acgcctggca ccgttcgcgc cacttcttca atttgctcag ccatcgcgtc
     5461 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
     5521 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagacg
     5581 cacggtgtta tccagttcct caatgatttt gtccattgtc atgcctggcc gccactgatc
     5641 ctgtggctta agctggatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     5701 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
     5761 gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
     5821 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     5881 cggccagaga accgtcagca ccgaaagcgc cgccaccagc agcgtggttt tcggccagtg
     5941 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcggattact
     6001 gctttccggc ggaattttgc cacggatcca gtaacccatc aggataggga tcactacgat
     6061 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccaacg ggccaaacag
     6121 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag
     6181 actgataaac agcgccggcc ccacttcaac agacgcatcg gtgatcacct gccagcgcgt
     6241 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
     6301 attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc
     6361 cagcgacata atattggcat tcagcccctg gaagggcatg acaataaaag caatacacaa
     6421 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
     6481 gcagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     6541 ccggtcaatg agctggctgc ggtcgtatgt tgtaactatc tccacgcctt ccggcagact
     6601 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc
     6661 ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgtttagtt cggcaatgcc
     6721 ccggcgcatc ttcgggccaa tctggacctt cgcaacatcg cgcagataaa cgggcacgcc
     6781 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgta gatagccgct
     6841 ggcgcgcacc atatattctg cttccgccag ttcgattgac gaaccgcccg cttcctggtt
     6901 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
     6961 aggatcgata accacctgat actctttcac cacaccgcct accgacgcca cttccgcaac
     7021 gtcaggaatg gttttcagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc
     7081 aagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     7141 ggcatctggt cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     7201 gtactccagc acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata
     7261 cacataagag tcgccaaact gcgagaaacc acgcacagtc ttcgcgccag gcaccgacaa
     7321 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctggccggg
     7381 atagctggtt ttaataatca cctgcacatc ggagagatcc ggcaacgcat ccactggcgt
     7441 attaatgatg gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa
     7501 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
     7561 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
     7621 atcaggaaca ggccgccgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg
     7681 gtgacgcctt gcgatgcctg gaaaacagca acgcgtttcg gtacaaagcg cccgtcggca
     7741 tcaacggtaa tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc
     7801 agcatcggtt cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc
     7861 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca
     7921 ggtagcagcg tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagg
     7981 gtaaactgcg aggcatcttt caccagccag gcgatggact ccgggatcgc agcagtgacc
     8041 cacaccgggt ccataccctg aattcgcgat tacgcgcaat ccccctgaga accgccatga
     8101 acaagggttt catgatttgt gattttagtt tggtacgcaa tttggtacac aacactattt
     8161 tcacttcagc gggtagtcat caaactcacc ataacgagca tcgttgatga tgtatgtgat
     8221 caccacaaat atcacatctg gactagttcc gtcttcatgc gca
//
LOCUS       DAJVRL010000142         8236 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVRL010000142 DAJVRL010000000
VERSION     DAJVRL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.142"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..1153
                     /locus_tag="OQN02_003552"
     CDS             <1..1153
                     /locus_tag="OQN02_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCS1644209.1"
                     /translation="MPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRAD
                     DLRDERSYRRRIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVS
                     DAERLMFSRELNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAG
                     FDEILRSPKLPAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTT
                     GFKRGDKISFIGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKE
                     EKAYANVNTSLADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSF
                     SIPGIGVNGIFATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            1198..1422
                     /locus_tag="OQN02_003553"
     CDS             1198..1422
                     /locus_tag="OQN02_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644210.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEEKQCKKPGRKPKAAADEPDNEG"
     gene            1400..1900
                     /locus_tag="OQN02_003554"
     CDS             1400..1900
                     /locus_tag="OQN02_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCS1644211.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            1900..3318
                     /locus_tag="OQN02_003555"
     CDS             1900..3318
                     /locus_tag="OQN02_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCS1644212.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            3318..4115
                     /locus_tag="OQN02_003556"
     CDS             3318..4115
                     /locus_tag="OQN02_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644213.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            4115..4570
                     /locus_tag="OQN02_003557"
     CDS             4115..4570
                     /locus_tag="OQN02_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCS1644214.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            4573..5181
                     /locus_tag="OQN02_003558"
     CDS             4573..5181
                     /locus_tag="OQN02_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCS1644215.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            5191..6522
                     /locus_tag="OQN02_003559"
     CDS             5191..6522
                     /locus_tag="OQN02_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCS1644216.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            6523..>8236
                     /locus_tag="OQN02_003560"
     CDS             6523..>8236
                     /locus_tag="OQN02_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1644217.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEK"
BASE COUNT         2170 a         2028 c         2156 g         1882 t
ORIGIN      
        1 gatgcctgtt gagcaggaag cgccaaccca gactggctgg gatttaactg gcaacgcaac
       61 cgggattctg gaactctccg tgaagtgcaa catgggcgat ccggataacg atttcttcga
      121 gcttcgtgca gatgacctgc gtgatgagcg ttcttaccgt cgccgcatcc aggcatccgc
      181 caaaaaactg gcgaataaca ttgagtcagc gattgccaaa caggcaactg aaatgggctc
      241 gcttgttgtt cacgataccc gcgcaattgg tccatcgact ggcctgcctg gctgggattt
      301 tgtgtctgat gcagagcgac tgatgttctc ccgtgagcta aaccgcgaca tgggcatcag
      361 ttacttcctg aaccctgacg attaccgcaa agcaggccgc aacctggtag atggtgacat
      421 cttcgggcgc gttcctgaag aagcatatcg aaacggtact attcagcgtc agattgctgg
      481 ctttgatgaa attcttcgct caccgaaact tccggcagtt accaagtcaa ccgcgactgg
      541 tgtaactgtt tctggtgcgc agaagtttaa gccgcaggca tacacccttg ataccgatgg
      601 taacaaagag aacgtcgaca accgtgttgc aacggtgacc gtatcctcca ccaccggatt
      661 taagcgcggc gacaaaatca gcttcattgg tgtgaagttc ctgtctcaga tggcgaagaa
      721 tgtgctgact gatgacgcga ctttctcaat cacccgtgtg atcgatggta ctcacatcga
      781 aatcacgccg aagccgattg cgctggatga cgcgtcactg acaaaagaag agaaggctta
      841 cgctaacgta aacacctctc ttgctgatac cactccggta aacgttctga acgtggcaac
      901 aaccaccgct aacgtgttct gggctgatga ctcaatccgc ctgctgtctc agccgatccc
      961 ggtaacccat gaactgtttg ctggcatgaa aacgtcttcc ttcagcattc caggtattgg
     1021 tgttaacggc atcttcgcaa cgcagggtga tatcaacact ctgtctggta agtgccgtat
     1081 tgctgtgtgg tattcagcat gtgctgtacg accagaggca attggtgttg gtctgcctaa
     1141 ccagaccgcg tgataaccag agggagcttc ggctcccgtt tttatctgga gacaagcatg
     1201 acacacatga tctttcgtca tggcgacatg aagaagtgga aaggcgttgg ctacgacttt
     1261 gaaatcgtga aagccgaaga gcttcaggaa tatctggatg ctggctggtt ttcacatcct
     1321 gatgaccttt tgaaggatgt tgcagagcca gaagaaaaac agtgtaaaaa gcctggtcga
     1381 aaacctaagg cggcagcaga tgaacctgac aacgaagggt gatttagttc ttgcggcatt
     1441 acgtaagctc ggtgttgcat caaatgccac gttaaccgat gtcgaaccgc agtctatgga
     1501 agacggcgtc aacgaccttg aaatgatgat ggctgaatgg cttggcggtg atgcgtcacc
     1561 tggtatcaac gttggctaca tttttgctga tgcagatgtt gctccggatc cgggcgatga
     1621 gcacggttta tcaaataacg ctatcaatgc cgtcattttc aaccttgcct gccgaattgc
     1681 tccagattat gcgctggaag cgtctgcaaa acttataacc actgccagat acgggaaaga
     1741 gcgactcgtc aaactgtctg caatggacag ggcaaaagca gctaaatgta agtccggtta
     1801 tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg aagtggaatg gttggaatta
     1861 cttccaccga aaggaacctt gcgataacgg gagcgaataa tgcctattca gcaacttccg
     1921 cttatgaaag gtgtcggcaa agacttccga aacgccgact atatcgacta tctgccagtg
     1981 aatatgctgg ctacacccaa agaaatcctg aacagcagcg gatatcttcg ctcattcccg
     2041 ggcattgcca aacgttctga cgtgaacggt gtatctcgcg gcgtcgagta caacatggcg
     2101 cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca aaggagaaag tgaagtcggt
     2161 gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc ggacatcaca ggcggtaggc
     2221 gttaatggtc aactggtcga gtatcgctat gatggcacgg tcaaaaccgt ctcaaactgg
     2281 cctacagaca gtggattcac tcagtatgag ttaggttcag ttcgcgacat tacgcgctta
     2341 cgtgggcgtt atgcgtggtc aaaagacgga actgattcat ggtttatcac tgaccttgaa
     2401 gacgaatctc atcctgaccg ttacagcgca caatatcgtg ccgagtctca gcctgacggc
     2461 atcatcggta tcgggacatg gcgagacttc atcgtctgct ttggttcatc gactattgaa
     2521 tatttctccc tgactggcgc aaccaccgtt ggtgccgctt tgtatgttgc acaaccatcg
     2581 ctgatggtgc agaaaggcat tgccggaact tactgcaaaa cgccattcgc tgattcgtat
     2641 gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt atatcatcga ctccggtcag
     2701 gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc gctcctacac tgctgatgaa
     2761 ctggctgatg gtgtgatgga atcgctgcga tttgatgctc atgagttgct gattatccac
     2821 cttccgcgtc acgtcctcgt gtacgacgca tcttcaagcg ccaatggccc acaatggtgt
     2881 gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta tcgacttcgt ttacgaaggc
     2941 aatcagataa cgtgcggcga taagctggaa tctgttaccg ggaaactgca attcgatatc
     3001 agcagccagt acgacaagca acaggaacac ctgctgttta ctccactctt caaagcggat
     3061 aacgccagag tgttcgacct tgaggttgaa tcgtcaactg gcgttgcgca gtacgccgac
     3121 cgcctttttc tctctgcaac cactgactgc atcaattacg ggcgtgagca gatgattgag
     3181 cagaatgaac cgttcgttta cgacaagcgt gttttgtgga agcgagtagg gcgcatcagg
     3241 aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac ctgtcactct gtctggcgct
     3301 cagataagga ttgagtaatg gctgattcga atctcaatgt gccggtaatc atccaagcta
     3361 cgcggctcga tacatcagtc cttccacgca atatcttctc gcagtcgtat ctgctttacg
     3421 ttatcgcaca gggcactgat gttggtaacg tggctaacaa agccaacgag gccggacagg
     3481 gcgcttatga tgcacaggtc aggaacgatg agcaggatgt gattctcgct gaccatgagc
     3541 agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga ggagcgaatc agacaggcag
     3601 aatcaactct tcaggaccat gaaacacgaa tagctcagaa tgaaagcgat atttcgtcgc
     3661 ttgacacaag agttcagtcg ctggagtcgc aggtttcaga ccatgaaacg cgcatcgatg
     3721 ctctggagta tgcaaccaca cgcaagaagt cagaggttgt ttactctggt gtatctgtaa
     3781 ccatcccgac agcgccgacc aaccttgtta gcctgctgaa aacgctcaca ccgtcatccg
     3841 gctcgttggc accattcttc gacaccgtta acaacaagat ggttgtgttc aacgagaaca
     3901 aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc cagcggaacc gccaacaggt
     3961 caatgcagct aaccttttcc ggctctgttc ctgacacact ggtcagcagt cgtaatgcgg
     4021 cgacaacaac cgacaacatc ctgttagcta cgttcttcag cgtggataaa gacggctttc
     4081 ttgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac caacgcgaaa
     4141 catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga gcaacaacgg
     4201 tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc acggtcagtt
     4261 tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc acgccatgta
     4321 cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc gatacattct
     4381 gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc gacacggtca
     4441 gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat acttcaaagg
     4501 cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact tcctgaatca
     4561 cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgtgcgg cgaggaacct
     4621 tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa gtatgcagca
     4681 gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat catgaaaaac
     4741 cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa cctttccagt
     4801 ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta taaagacctt
     4861 gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg acttggttcg
     4921 acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc ttggttatca
     4981 aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct gcaaggtcag
     5041 gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca aagcgcagct
     5101 cttgccagcc ttttgggaac atcaacgcct tggggcgctg gtattggtgc tggtatcgga
     5161 ttgcttggct cgttgtttta aggggtaatc atggctactt ggcaaggaac aaacggcgga
     5221 ttgttggctg gtatcggcgg cgtcaactca aacgctccga gcgtaaatga catcggcaat
     5281 acgcttcagc ttatcaggca gaacaatgat attgagcgtt caggcgctaa caatgttggg
     5341 ctgactgctt tgcaaggtct ttcaggtatt gcgggtgttt ttcagcagga aaagcaggct
     5401 cagcggcaga aagaatttca gcaggcgtac gctaatgctt atgcgtctgg tgatcgcggt
     5461 gctttgcgtc agttggctac tcaatatcca gaccagattg aatccgttcg taaaggcatg
     5521 ggattcattg atgaagagca gcgtaattct atcggcacct tagcggctgg cgcacgcctt
     5581 gcgtcatcgt ctccagaagc aatgcaatca tggctgcaaa acaacgccgg tgagttagct
     5641 cgtgttggcg ttaatcctca tgacgtcgct cagatgtacc aacagaaccc gcggcagttc
     5701 ggcgaatttg tcgatcacct ggggatgaac agtctcgggc ccgaaaaata ctttgaccta
     5761 caggataaaa tgcagggtcg ccaggttacc atgcgcggtc aggatctgga ttcgcaaacc
     5821 gccgctcgga atcaggcaat cacaatgcgc ggacaagata tccaggcgaa tttaggtcag
     5881 cagcgcatta atctggacgc agaaacaaac cgcattaaca acgaaaataa gcgccttgac
     5941 cggatgctat cagcagaaac taacgacctg aagcgccagg aaatacagag ccgcatagca
     6001 gccaacaacc agcagttgca gcagaagcag caagcgctaa atgatggcta caaagacggc
     6061 atcaacaccc tcacaaccag catgttcact ctgaacgata tcgttagttc tccttcactt
     6121 aagagcatta caggcttacg tggagtaatc cccaacgttc caggctcaca ggctgcagac
     6181 actcaggcac gacttgatac ctttaaatca caagcatacc tgacagcggt tcaggccatg
     6241 cgaggcatgg gcgcactttc tgatgccgag ggcaaaaagc tcgaccaggc tgttggttcg
     6301 ctgcagaact cgcagagcga ggagtccttt cgtcgcaacg ctggcgtcat cctgaacacg
     6361 ctcaaccaga agcgtaatga ggcggttggt aagtacgttc agcaaaacgg tatcaagcga
     6421 gtggaagcgc ctcaggcttc tatagattac ctgaagcagc accccgagct gtcaatcgac
     6481 ttcattaatc gctacggata tcttccatct ttggggcagt aaatggctaa ttaccgtgat
     6541 ttgttagagc aggctggcgc acgttacggt gtgccagaag ggttgatgac tgcactgggt
     6601 gccaaggagt cttcttacaa ccctgccgca gtaagctccg ccggggctgt aggattgact
     6661 caggtcatgc ctgggacatg gcgtgatatg ggttataccg atgagcaaat gcaaaacccc
     6721 gaatatcagg ctgacgctgg cgcgcgctat ctggcaaaga tgtaccagca gtttggtaac
     6781 tggcgtgacg ctcttcaggc ttatcacgac ggtcccggca acgttatgaa ggcaaagcgt
     6841 ggtgaatata cgccaggacc tgaaggccgc ggttacgttg atgatcgctt tgctcaatgg
     6901 gctggtgacc cggtgacaga ctcaacagtc gaacagcgcg ccacctctgc aaaggtacat
     6961 cctcagcaag accctaacaa cccgtttgca caactggaag tacagtcatc cgaacaagta
     7021 tcggcatcag gtgtgcagtc agacccaaat aatccatttg ctcagattga gcagcaggca
     7081 gccagtcagc agccacctca acccgtaagt tctgtcgcac cgaaacctgt tcagcaacaa
     7141 acagttaatc aggccaataa tgcaccaaca cgggaagaac catcattgat gcaacaagct
     7201 ggcgattggc tcactggtgg tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt
     7261 ctggtaaaca taccatttga cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt
     7321 caggggcttg gggggccaaa agtattggat gatgtttatc gtccagtaga cagaccgaca
     7381 gacccctacg cgcaagctgg agagtcaata ggcggttatc ttgttcctgg agcaggtgta
     7441 gctggaaaca tggtcattgg ttctctcgct gacgcggcga atcaacgggg tgattttgcc
     7501 gaaaatgccg ctattaatgc cggacttagc attgctacgc atggcctgat aaatggcgtt
     7561 acccgtggtg ttcgtggtgc atcaaatata attagtggca ataaaacatc tgcacagaga
     7621 gcgaccactg cgccaacaga aacatcacca ttctccggtg atgccgctgc agcaacaaat
     7681 cctgcggttc atgccgcaga ggcaagagta gcacaaggtg taccaatgac gcctgcgacg
     7741 aagaacccag aggaagtcgt tcgcacagta gcagcacaaa aaaggccaaa tctcgcttca
     7801 tcgcttgatg aactagatat caatcctcag gctgaagttc tggagtctgc tgaaaggctt
     7861 aatgttgatt cattactccc ttcacacttt tccgggaacg agcaatacaa ggcagttgag
     7921 caagcaatca agtcccgtgc gggttctgct ctacaggtgc aggaaaatga agcaatcagg
     7981 cagctagcac agggcgcggg tgagataatt gatcgcgttt ccggtgcaaa agatgctctt
     8041 ggtatgagcg acaagtttat tgatacggtc aatggaagaa tgtctgcgct gatgaaacga
     8101 agcgaccagc tttatcgcaa tgttgaaaag gcgatgcctg caggtgcaaa aattgatgcg
     8161 ccatcaacaa ggtcaatgct caaacaggtg gcagaagatc ttggcgggat gaaaaacctt
     8221 gaccctattg aaaaga
//
LOCUS       DAJVRL010000143         8208 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVRL010000143 DAJVRL010000000
VERSION     DAJVRL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.143"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..304
                     /locus_tag="OQN02_003561"
     CDS             <1..304
                     /locus_tag="OQN02_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCS1644218.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2204
                     /locus_tag="OQN02_003562"
     CDS             276..2204
                     /locus_tag="OQN02_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCS1644219.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2188..2391
                     /locus_tag="OQN02_003563"
     CDS             2188..2391
                     /locus_tag="OQN02_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1644220.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="OQN02_003564"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="OQN02_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="OQN02_003565"
     CDS             3971..5476
                     /locus_tag="OQN02_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS1644221.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="OQN02_003566"
     CDS             5513..5860
                     /locus_tag="OQN02_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1644222.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="OQN02_003567"
     CDS             5918..6946
                     /locus_tag="OQN02_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1644223.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="OQN02_003568"
     CDS             6998..7384
                     /locus_tag="OQN02_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1644224.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="OQN02_003569"
     CDS             7396..7773
                     /locus_tag="OQN02_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1644225.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..>8208
                     /locus_tag="OQN02_003570"
     CDS             7760..>8208
                     /locus_tag="OQN02_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1644226.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPI"
BASE COUNT         1898 a         1933 c         2539 g         1838 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgccg tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gcgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc tgggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tgttttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gctggcggca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ccaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgtgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 ggctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagtcctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggcagcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacgacccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattga
//
LOCUS       DAJVRL010000144         7916 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVRL010000144 DAJVRL010000000
VERSION     DAJVRL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.144"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            237..740
                     /gene="dps"
                     /locus_tag="OQN02_003571"
                     /gene_synonym="pexB"
     CDS             237..740
                     /gene="dps"
                     /locus_tag="OQN02_003571"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1644227.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1144..1890
                     /gene="glnH"
                     /locus_tag="OQN02_003572"
     CDS             1144..1890
                     /gene="glnH"
                     /locus_tag="OQN02_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1644228.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2029..2688
                     /gene="glnP"
                     /locus_tag="OQN02_003573"
     CDS             2029..2688
                     /gene="glnP"
                     /locus_tag="OQN02_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1644229.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2685..3407
                     /gene="glnQ"
                     /locus_tag="OQN02_003574"
     CDS             2685..3407
                     /gene="glnQ"
                     /locus_tag="OQN02_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1644230.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3668..5892
                     /gene="ybiO"
                     /locus_tag="OQN02_003575"
                     /pseudo
     CDS             3668..5892
                     /gene="ybiO"
                     /locus_tag="OQN02_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="OQN02_003576"
     CDS             complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="OQN02_003576"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1644231.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7091..7351
                     /locus_tag="OQN02_003577"
     CDS             7091..7351
                     /locus_tag="OQN02_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1644232.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7616..>7916
                     /locus_tag="OQN02_003578"
     CDS             7616..>7916
                     /locus_tag="OQN02_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1644233.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1934 a         2025 c         1978 g         1979 t
ORIGIN      
        1 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
       61 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
      121 tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
      181 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
      241 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
      301 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
      361 atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg
      421 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
      481 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
      541 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
      601 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
      661 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
      721 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
      781 gcggcttcct gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg
      841 catccatctg ctatccccag ctgattaagt aaattttttg tatccacatc atcacacaat
      901 cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc
      961 acaattttag cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat
     1021 aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa tgagttgcaa aattggcacg
     1081 attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat
     1141 gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct
     1201 tctcatgccg cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa
     1261 tttaaacagg gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa
     1321 gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat tccggcactg
     1381 caaaccaaaa acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa
     1441 gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac
     1501 aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt gaagagcggt
     1561 actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg
     1621 aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat
     1681 acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt
     1741 gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt
     1801 gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac
     1861 aaaaaatggt tcagtactga accgaaataa taacgctaca cctgtaaagc gcactggcag
     1921 ttccctctcc cccctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat
     1981 taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac
     2041 tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg
     2101 atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc
     2161 accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt gatccgcggc
     2221 acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc gtttaacgac
     2281 ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac
     2341 attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca aaggttttcg tgaagcagga
     2401 ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg
     2461 cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg
     2521 tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac
     2581 ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg
     2641 ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa
     2701 acgtctccaa gcactttggc ccaacccagg tgctgcacaa tatcgatttg aacattgccc
     2761 agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct
     2821 gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtca
     2881 acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt
     2941 tttacctctt cccgcatctg acggcgctgg gaaacgtcat gtttggtccg ctacgcgtgc
     3001 gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc
     3061 tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg
     3121 cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg
     3181 ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag
     3241 ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc
     3301 tgatctttat cgacaaaggc cggattgcgg aagatggcaa tccgcaggtg ttgatcaaga
     3361 acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaataa gacacattgc
     3421 ctgatgcgct acgcttatca ggcctacagg atatctggca acttattaaa attgcatgaa
     3481 cttgtaggac ggataaggcg ttcacgccgc atccggcaaa aaagcccgca cgttgtcagc
     3541 aacctgctta atatcccttc ctccctttca cccgaaaggg aggcacacca gattcctctc
     3601 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
     3661 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
     3721 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
     3781 ggaaccggat atcgaacaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
     3841 gacacctcgc gtaaagagtt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
     3901 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
     3961 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
     4021 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
     4081 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
     4141 gcactgccgc tatatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
     4201 aactggttgc agcttccggc gatgattatt ggggcgttta ttatcgacct gctgttactg
     4261 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
     4321 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
     4381 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
     4441 gagagcgccc gttactgggg tcgtcgcctg agctggttaa gcagcctgat tggctatggc
     4501 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
     4561 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt ccgaaataaa
     4621 aaagagatta cccagcatat gctcaacttt gcggagcatt cgctggcttt tttcagcctg
     4681 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcatgctg
     4741 tttttctttt cgttgttcga tccgggcaac agtctgaaat ttatgatggg tgcaacggtg
     4801 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
     4861 gcgaaaacca tcaccctctc gccacatact cagcgtaact atccggagct acaaaaacgg
     4921 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggca
     4981 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
     5041 gcggggcaga aaaccgtaga tatcctgatc cgcatcgcac tcattctttt cttctcggcg
     5101 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
     5161 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
     5221 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
     5281 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
     5341 gtaaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat
     5401 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
     5461 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
     5521 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
     5581 gctgataaag ccaatcaggc actgaaagat gcggtaacgg aattgatgga aaacgaagaa
     5641 attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt aagcaatacc
     5701 gcgtttacac tgcatgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
     5761 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
     5821 acttatcagg tgctgcctgc tccgggcgcg accccggctg aaccgctgcc gccgggggag
     5881 ccaacgcttt aacgttggcg attaacaaaa cggcggcgct gctcgtcgtt cataaaggtc
     5941 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
     6001 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
     6061 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
     6121 aagttgactt caccgccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
     6181 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgccgcagcg
     6241 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttgtggatg
     6301 atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
     6361 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
     6421 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
     6481 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
     6541 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
     6601 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
     6661 accgccagcg gattggcaaa gtctacgctt tgctcccctg cgggtgtaag cgtgaggaat
     6721 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
     6781 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
     6841 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
     6901 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga
     6961 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag catatctctg tcaatttttt
     7021 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
     7081 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
     7141 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
     7201 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
     7261 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
     7321 gatgttaggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
     7381 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa
     7441 atgtatttct ttttcgcgca atgggtgata gaaaatcgca tcaagtgata atgcttatca
     7501 aaattactat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
     7561 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
     7621 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
     7681 tattggtatt acgcctgtgg cacaggcact cgccgccgaa gggcaagcta acgcggatga
     7741 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
     7801 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
     7861 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc ccggcg
//
LOCUS       DAJVRL010000145         7838 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVRL010000145 DAJVRL010000000
VERSION     DAJVRL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.145"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            49..1388
                     /gene="yjeM"
                     /locus_tag="OQN02_003579"
                     /pseudo
     CDS             49..1388
                     /gene="yjeM"
                     /locus_tag="OQN02_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="OQN02_003580"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="OQN02_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1644234.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="psd"
                     /locus_tag="OQN02_003581"
     CDS             complement(4872..5840)
                     /gene="psd"
                     /locus_tag="OQN02_003581"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1644235.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="OQN02_003582"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="OQN02_003582"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1644236.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="OQN02_003583"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="OQN02_003583"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1644237.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1782 a         2038 c         2110 g         1908 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga gagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagta
//
LOCUS       DAJVRL010000146         7780 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVRL010000146 DAJVRL010000000
VERSION     DAJVRL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.146"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            54..587
                     /gene="ymdB"
                     /locus_tag="OQN02_003584"
     CDS             54..587
                     /gene="ymdB"
                     /locus_tag="OQN02_003584"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1644238.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            529..2010
                     /gene="clsC"
                     /locus_tag="OQN02_003585"
     CDS             529..2010
                     /gene="clsC"
                     /locus_tag="OQN02_003585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS1644239.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="OQN02_003586"
     CDS             complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="OQN02_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1644240.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3569..5104
                     /gene="mdoG"
                     /locus_tag="OQN02_003587"
     CDS             3569..5104
                     /gene="mdoG"
                     /locus_tag="OQN02_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1644241.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5097..7640
                     /gene="mdoH"
                     /locus_tag="OQN02_003588"
     CDS             5097..7640
                     /gene="mdoH"
                     /locus_tag="OQN02_003588"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1644242.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1926 a         1863 c         2058 g         1933 t
ORIGIN      
        1 taaccaaaaa gcctgctgta cacttaagaa acaagaaggt gtaaggaggc gttatgaaaa
       61 cgcgtattca tgttgtgcag ggtgatatta ccaaactggc cgttgatgtg attgtgaatg
      121 cggctaatcc gtcattaatg ggaggcggcg gcgtcgatgg ggccattcat cgcgcagcgg
      181 gtccggccct gctggatgcc tgtttaaaag tcaggcaaca gcagggcgat tgccctacgg
      241 ggcatgccgt tattacgctt gcaggcgatc ttcccgctaa agccgtagta cacaccgtcg
      301 ggccagtctg gcgtggtggg gaacaaaacg aagaccagct tttgcaggat gcctatctga
      361 atagcctacg actggtggcg gcaaacagct atacgtcagt ggcttttcct gcaatcagta
      421 ctggggttta tagttaccct cgtgcggcag cggctgaaat cgcagttaaa accgtttcag
      481 aatttattac ccgtcacgct ttacccgaac aggtatactt tgtctgttat gatgaagaaa
      541 acgcccacct ctacgaaaga ctccttaccc aacaaggaga tgaatgattt gccccggctg
      601 gcgagcgcgg tgctgccact gtgttcgcaa catcccggtc agtgtggtct tttccctctg
      661 gagaaaagtc tggatgcgtt tgccgcccgg tatcgtctgg ccgaaatggc agagcatacg
      721 ctggatgttc agtattacat ctggcaggac gatatgtcgg ggcggttact gttttccgcc
      781 ctgttagccg cagcaaagcg tggcgttcgc gtccgtttgt tgctggacga caacaatacg
      841 cccggacttg acgacatttt gcgcttgctt gacagtcatc cacgcattga agtccggctt
      901 tttaatcctt tctcgtttcg cttgctacgt ccgcttggat atatcaccga cttttcccgt
      961 ctcaatcgcc ggatgcacaa taaaagtttc actgtcgatg gcgtggttac cctagtggga
     1021 ggacgaaata ttggtgatgc ctattttggt gcaggggaag agccactttt ttcggattta
     1081 gatgtcatgg caataggccc cgtggtagag gatgttgccg atgatttcgc ccgctactgg
     1141 tattgcaaat cggtttcacc cttacagcag gtgctggatg tcccggaagg tgaaatggcg
     1201 gatcgcatcg agttacccgc ctcctggcat aacgatgcca tgacgcatcg ttatttacgc
     1261 aaaatggaat ccagtccatt tataaatcat ctggttgatg gaacgttgcc gcttatctgg
     1321 gcgaagacac gtttattaag tgatgatccg gcgaaagggg agggcaaggc aaaacggcat
     1381 tcactgttac cgcagcgcct gttcgatatc atgggctcac ccagtgaacg catcgatatt
     1441 atctcttcct attttgtacc gacacgcgca ggtgtggcgc aactcttacg gatggtgaga
     1501 aaaggggtaa agattgcgat cctaaccaat tctcttgccg ctaacgatgt tgctgtcgtc
     1561 catgccggat acgcgcgctg gcgcaaaaaa ttgctccgct atggcgtgga attatatgaa
     1621 ctcaagccga cgcgtgaaca aagcagtacg ttacacgatc gcggcataac cggtaattcc
     1681 ggtgccagcc tgcatgctaa aacttttagc atcgatggta aaacggtgtt tatcggctct
     1741 ttcaatttcg atccgcgttc aacattgctc aatactgaaa tgggcttcgt gatagagagc
     1801 gaaacgctgg cacagttaat tgataaacgc tttattcaga gccagtacga tgcggcctgg
     1861 cagctccgtc tggacaggtg gggacggatc aactgggttg atcgtcatgc aaagaaagag
     1921 gttattctca aaaaagaacc cgccaccagt ttctggaagc gggttatggt cagactggcg
     1981 tcgatattgc ccgtggaatg gttattgtaa atgtggctta acgggctggt gctttatcgt
     2041 tctcacgctt aacaaccggt ttaccagaga acaaaaactt cagtaacggg atgcgtaaat
     2101 gaatttcata cagaattatc gcaatcccta ctacgaatat caggccacag agaaaaccaa
     2161 gccagttgga ggtgatgtgc ggtgtaatgt atgcgccgaa aaacagcgtt aacgggtggt
     2221 gaaccagata gataaacagc gatgcgttaa caaaataagt cacccgcgct gactggaagt
     2281 taagcaaacg gtggccaaag gagaagacca cattcaccat ccacaggccg aggaccatgg
     2341 tgatcaccga ctcggtttcg tacatccagg catcgccact gccatagcgc tggttgagta
     2401 aataagcgac aaacgccaat gctgcagcga gggtacagcc acgagacggc gtggtaaaca
     2461 aggctttaag atgagggaaa atgaaagcca gtgcgccgag gataaagaac ggtaaataaa
     2521 atagcgtttg catgacaata aaattgaaca tgccattact cagaatgggt ggatacacaa
     2581 taaaaatcgt tcttcttatt accgcatagc cgatgccgag gcataaaaaa atcaccgata
     2641 gttttaccat cgagaatttt ttattcgttt tatcagaatt ttctaaatta tttctgatgc
     2701 gcttaaatat ccatacacac agcgtcgtca tgactactaa caccagtaaa aaccacaggt
     2761 gtgatattaa ttcccaggcc aacgtattat atttgtcata caatgatagc ccaggccaac
     2821 tttccgcttt ccctttgaca tattgcagca taataaattg cggtaatgtc agtaggggga
     2881 tggctgttaa catcgggata cctacacgtt cgacacgtac tttccaccat tttttcaagg
     2941 gatagcgtaa aaaaagcatg taggaaaagt atccggatat aacgaaaaat acctgcatgc
     3001 ggaacgagtg gatgaagtca ttaaaaaggg ttagccacaa tgacggttcg gcgctattca
     3061 catgccatgt atggctcgaa tagattaaag aaatatgaaa agggatccct aacaacatca
     3121 gccaggcgcg gatggagtcg aggaaatatt cacgttgcgc gggtactgga ttcatatatg
     3181 gttaactaat ctcggatttt tcgtcttatc cctgtcgggt tatgccttta ggcttgttgc
     3241 catagcgaca ccgacctgac cgcgccaggc gcaggcttca aggtttttat gcatagcatc
     3301 atcgctacca ctaaccagaa tggaagcgtc tgtaagacgg ttgataaata aatttgctgg
     3361 caaaccctac acgaagtcga tgcttctgtc tttaggagaa gcacggaaag tgaaaacggt
     3421 tgcaatcagg tgcttaatcc atgagccagc gtgctgaacg ataccgggat tcagttgtcg
     3481 gaatggctgg ttatccatta aaatagatcg gatcgatata agcacacaaa gggggaagtg
     3541 cttactaatt atgaaacata aactacaaat gatgaaaatg cgttggttga gtgctgcagt
     3601 aatgttaacc ctgtatacat cttcaagctg ggctttcagt attgatgatg tcgcaaagca
     3661 agctcaatcc ttagccggga aaggctatga ggcgcctaaa agcaacttgc cctccgtttt
     3721 ccgcgacatg aaatacgcgg actatcagca gatccagttt aatcatgaca aagcgtactg
     3781 gaacaatctg aagaccccat tcaaactcga gttctaccat cagggtatgt acttcgatac
     3841 cccggtcaaa ataaatgaag tgactgccac cgcagtcaaa cgaatcaaat acagcccgga
     3901 ttatttcact ttcggcgatg ttcagcatga caaagacacg gtaaaagacc ttggttttgc
     3961 cggttttaaa gtgctttacc cgatcaacag caaagataaa aacgatgaaa tcgtcagcat
     4021 gctcggggcc agctatttcc gcgtgattgg tgcaggtcag gtttatggcc tttctgcccg
     4081 cggcctagca attgataccg ctttgccatc gggtgaagaa tttccacgct tcaaagagtt
     4141 ctggatcgag cgtccaaaac cgactgataa acgtttaacc atttatgcat tgcttgactc
     4201 gccgcgtgcg acaggtgctt acaaattcgt ggttatgcca gggcgtgaca cggttgtgga
     4261 tgtgcagtcg aaaatctatc tgcgcgataa agtcggcaaa ctgggggttg caccgttaac
     4321 cagtatgttc ctgtttgggc cgaaccaacc gtcgcctgca aataactatc gtccggagtt
     4381 gcacgactct aacggtctgt ctatccatgc tggtaatggc gaatggatct ggcgtccgtt
     4441 gaataacccg aaacatttag cggtcagcag cttctccatg gaaaacccgc gaggctttgg
     4501 tctgttgcaa cgaggtcgtg atttctcccg ctttgaagat ctcgatgatc gttacgatct
     4561 ccgtccaagc gcatgggtga ctccgaaagg ggagtggggc aaaggcagcg ttgagctggt
     4621 ggaaattcca accaacgatg aaaccaacga taacatcgtc gcttactgga cgccggatca
     4681 gctgccggag ccgggtaaag agatgaactt taaatacacc atcaccttca gccgtgatga
     4741 agacaaactg catgcgccag ataacgcatg ggtgcaacaa acgcgtcgtt caacggggga
     4801 tgtgaagcag tcgaacctga ttcgccagcc tgacggtact atcgcctttg tggtcgattt
     4861 taccggcgca gagatgaaaa aactgccaga ggataccccg gtcacagcgc aaaccagcat
     4921 tggtgataat ggtgagatag ttgaaagcac ggtgcgctat aacccggtta ccaaaggctg
     4981 gcgtctggtg atgcgtgtga aagtgaaaga tgccaagaaa atcactgaaa tgcgtgctgc
     5041 gctggtgaat gccgatcaga cgttgagtga aacctggagc taccagttac ctgccaatga
     5101 ataagacaac tgagtacatt gacgcaatgc ccatcgccgc aagcgagaaa gcagcattgc
     5161 cgaagactga tatccgcgcc gttcatcagg cgctggatgc cgaacaccgc acctgggcgc
     5221 gggaggatga ttccccgcaa ggctcggtaa aggcgcgtct ggaacaagcc tggccagatt
     5281 cacttgctga tggacagtta attaaagacg acgaagggcg cgatcagctt aaggcgatgc
     5341 cagaagcaaa acgctcctcg atgtttcccg acccgtggcg taccaacccg gtaggccgtt
     5401 tctgggatcg cctgcgtgga cgcgatgtca cgccgcgcta tctggctcgt ttgaccaaag
     5461 aagagcagga gagcgagcaa aagtggcgta ccgttggtac catccgccgt tacattctgt
     5521 tgatcctgac gctcgcgcaa actgttgtcg cgacctggta tatgaagacc attcttcctt
     5581 atcagggttg ggcgctgatt aatcctatgg atatggttgg tcaggatttg tgggtttcct
     5641 ttatgcagct tctgccttat atgctgcaaa ccggtatcct gatccttttt gcggtactgt
     5701 tctgttgggt gtccgccgga ttctggacgg cgttgatggg cttcctgcaa ctgcttattg
     5761 gtcgcgataa atacagtata tctgcgtcaa cagttggcga tgaaccatta aacccggagc
     5821 atcgcacggc gttgatcatg cctatctgta acgaagacgt gaaccgtgtt tttgctggct
     5881 tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc caaacacttt gatgtctaca
     5941 ttctgagtga cagttataac ccggatatct gcgtcgcaga gcaaaaagcc tggatggagc
     6001 ttatcgctga agtcggtggc gaaggtcaga ttttctatcg ccgccgccgt cgccgcgtga
     6061 agcgtaaaag cggtaatatc gatgacttct gccgtcgctg gggcagccag tacagctaca
     6121 tggtggtgct ggatgctgac tcggtaatga ccggtgattg tttatgcggc ctggtgcgct
     6181 tgatggaagc caacccgaac gccgggatca ttcagtcgtc gccgaaagcg tccggtatgg
     6241 atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca ctgtttacag
     6301 ccggtttgca cttctggcaa cttggcgagt cgcactactg gggacataac gcgattatcc
     6361 gcgtgaaacc atttatcgag cactgcgcac tggctccgct gccgggcgaa ggttcctttg
     6421 ccggttcaat cctgtcacat gacttcgtag aagcggcgtt gatgcgccgt gcaggttggg
     6481 gggtctggat tgcttacgat ctcccgggtt cttatgaaga attgccgcct aacttgcttg
     6541 atgagctaaa acgtgaccgc cgctggtgcc acggtaacct gatgaacttc cgtctgttcc
     6601 tggttaaggg tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg atgtcttatc
     6661 tctccgctcc gctgtggttt atgttcctcg cgctctctac tgcattgcag gtagtgcatg
     6721 cgttgaccga accgcaatac ttcctgcaac cacggcagtt gttcccggtg tggccgcagt
     6781 ggcgtcctga gctggcgatt gcactttttg cttcgaccat ggtgctgttg ttcctgccga
     6841 agttattgag cattttgctt atctggtgca aaggaacgaa agaatacggc ggcttctggc
     6901 gcgttacatt atcgttgctg ctggaagtgc tgttttccgt gctgctggct ccggtacgca
     6961 tgctgttcca tacggtcttc gttgtcagcg cgttccttgg ctgggaagtg gtgtggaatt
     7021 caccgcagcg tgatgatgac tccacttcat ggggcgaagc gttcaaacgc cacggctcac
     7081 agctgctgtt ggggttagtg tgggctgttg ggatggcgtg gctggatctg cgtttcctgt
     7141 tctggctggc accgattgtc ttctcgttga tcctgtcacc gtttgtttcg gtgatttcca
     7201 gccgtgccac cgttggtctg cgcaccaaac gctggaaact gttcctgatc ccggaagagt
     7261 attcaccgcc gcaggtgctg gttgataccg atcggttcct tgagatgaat cgccaacgct
     7321 cccttgacga tggctttatg cacgcagtgt ttaacccgtc atttaacgct ctggcaaccg
     7381 caatggcgac agcgcgtcac cgcgccagta aggtgctgga aatcgcccgt gaccgccacg
     7441 ttgaacaggc gcttaacgag acgccagaga agctgaatcg cgatcgtcgc ctggtgctgc
     7501 taagcgatcc ggtgacgatg gcccgtctgc atttccgcgt ctggaattcc cctgaaagat
     7561 attcttcatg ggtgagttat tacgaaggga taaagctcaa tccactggca ttgcgtaaac
     7621 cggatgcggc ttcgcaataa aaacgtagtt gcctgatgcg ctacgcttat caggcctaca
     7681 tcgttcctgc aatttattga ttttgcaaga ttttgtaggt cggataaggc gttcacgcca
     7741 catccggcaa aaacaacgag ccaataaaaa taccggcgtt
//
LOCUS       DAJVRL010000147         7747 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVRL010000147 DAJVRL010000000
VERSION     DAJVRL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.147"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(43..522)
                     /gene="xdhC"
                     /locus_tag="OQN02_003589"
     CDS             complement(43..522)
                     /gene="xdhC"
                     /locus_tag="OQN02_003589"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS1644243.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(519..1397)
                     /gene="xdhB"
                     /locus_tag="OQN02_003590"
     CDS             complement(519..1397)
                     /gene="xdhB"
                     /locus_tag="OQN02_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1644244.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1408..3705)
                     /gene="xdhA"
                     /locus_tag="OQN02_003591"
     CDS             complement(1408..3705)
                     /gene="xdhA"
                     /locus_tag="OQN02_003591"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1644245.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4120..4875
                     /locus_tag="OQN02_003592"
     CDS             4120..4875
                     /locus_tag="OQN02_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1644246.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4954..5027
                     /locus_tag="OQN02_003593"
     tRNA            4954..5027
                     /locus_tag="OQN02_003593"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4986..4988,aa:Gly,seq:ccc)
     gene            5142..6746
                     /locus_tag="OQN02_003594"
     CDS             5142..6746
                     /locus_tag="OQN02_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1644247.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
     gene            7586..>7747
                     /locus_tag="OQN02_003595"
     CDS             7586..>7747
                     /locus_tag="OQN02_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1644248.1"
                     /translation="MSQQPQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLR
                     DIRKMLEELN"
BASE COUNT         1981 a         1887 c         1960 g         1919 t
ORIGIN      
        1 taacctcagt gaatccggcc tgaattcagg ccggatatcc ttttacttcg ttttctcgca
       61 atccagaact gtatttacaa tcatctgata ccccgtgcag cgacaaagat ttcccgccag
      121 tccgcgacga atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca gcattgccgt
      181 ggtagccata atcaggccag gcgtacaaaa cccgcactgc actgtgccgg atttcgcata
      241 agcctgctga acatgagaaa gttttccgcc tttcgcttca ccttccagcg tgcggatctc
      301 ttttccttca gcccaggcgg caaggtataa gcaactgtct attgctgtgc cgtcgaccaa
      361 caccgtacag gcaccacatt cacccacgca gcacccttgt ttgacactta gcagtccttg
      421 ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg tgaagctgaa aaggcatccc
      481 gttaatggtg cattcggtgg taattgtttc gctgtgattc attgcaattt tccccccgcc
      541 gcggcgacgg cttcgctaat cacttttttg gtcatcgtct ggatgagatg cagacgaaac
      601 tctttactgg cccgccatga agaacgcggg gcgacatctt gcaggacaga ttcgctgata
      661 gcttccagcg tttgcaggtt taatggcgca ttttgtgcag tctgttcggc atgttggcag
      721 cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta attcgctgaa attgccgtta
      781 tccagtcggc aatgtgcggc gcagccaatc gttgaaatat ccattgcgtc gcgcatggca
      841 tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg gcggaaaatg aaaggcgacg
      901 aggatttcgt catgctcaag agacactttg cccggcccgg tgtgaaagcc attaatcggg
      961 acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg cgtcataaat tagcgttggc
     1021 gtggcagaat ctgcgctggt ggcaccgttg caaatatttc caccgtaggt agcgacgtta
     1081 cggatctgcg gtccagcaat gaacgtggcc gcagcacata acgccgggag atgacgttga
     1141 gttataggat cttctattag ctgggtaaat gtcgttgcag agccgatacg tagcgagcca
     1201 tcttccgcca gcgtaattcc ccgcagctcc gccagattat gaatatcaac aatatggcga
     1261 taacggtcat tgtgatggtg gagctgaatc agtacgtcag tgccaccggc gagcagtttg
     1321 gcctgcgggt tgtcagccag caggttgatg gcatcggcaa gggttgctgc gcgatggtaa
     1381 gaagcaaaat caaacatgat gttatcctca aatcaatcct gccagatgga actcttcata
     1441 taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca tcttcaccgc
     1501 gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact tatgtccgta
     1561 tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca gatccggcat
     1621 ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt tagcatcgat
     1681 gatcatctct tcaaatagcg cccagtcaat gcccattccc attccgccgt gtacctgacc
     1741 ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga tgcggttgat
     1801 ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc caaacgccgg
     1861 tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc gttcagggtg
     1921 gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg gtcgttcaac
     1981 cagcacgata tggcctttta tcagggtcaa attcatcgct gactgatgta gcatgactgc
     2041 ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg caggcgcggc
     2101 aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt cggtatcttg
     2161 tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca ccatttgcga
     2221 gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt tgatggttcc
     2281 atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc aggtgttaga
     2341 ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct ggttctggca
     2401 ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac actccggcaa
     2461 ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc gggcggcgtt
     2521 gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat caagcataga
     2581 ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag ccgaggggag
     2641 gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa ggtaagcgac
     2701 tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc cggtgttaga
     2761 cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca tttgcccgtc
     2821 aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc taagggaaac
     2881 tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct cttccagtac
     2941 atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta cgcatgacca
     3001 gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct gggtgctgga
     3061 aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc tttccatgtg
     3121 acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg cggcatcgat
     3181 tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc cgccgttatg
     3241 gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg gtaattcttg
     3301 ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc gggccacgac
     3361 gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc gatcggcggt
     3421 atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg gaatatcagg
     3481 cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt gttcatcatt
     3541 aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt aacacatgcc
     3601 cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa tggcatcgac
     3661 gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa tcccctcgat
     3721 ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt acgccagaaa
     3781 cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca cagatatgat
     3841 acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc gataaaatca
     3901 atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa gtcaggaact
     3961 tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa tgaaattaac
     4021 ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag aggctggctg
     4081 ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg acgcctgaat
     4141 aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc gggctgttcg
     4201 ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt gaaacggggg
     4261 gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct ggcgcgactg
     4321 aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact gggtggggcg
     4381 aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac cgcatcggtt
     4441 acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg ttgttggtta
     4501 tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg caataaaggg
     4561 attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg aaaggtggtg
     4621 tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca cagtgaagat
     4681 tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca aagcgtgaag
     4741 gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg cctgcatttc
     4801 cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc gcagggcagc
     4861 aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt gctaaggaga
     4921 gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg gtagaacgag
     4981 agcttcccaa gctctatacg agggttcgat tcccttcgtc cgctccatga cctttcttaa
     5041 agtactccaa cctactgaat cctaagggat atggctttaa gcctccttgc agagttactt
     5101 actgtaaccc actgtgtaac cctctcaggg catacagaca tatgattcag ctatccagta
     5161 gtcacaaact tccagcagtc tactatctct accagcgtaa cggcgtctac tacttccgtc
     5221 ttcgggtcag acaatcaaac aatgatagaa tgacctcagt atcattacgg accaaggata
     5281 gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc actcaaggca atacacgcag
     5341 acagacccaa cgctacctat gaggagatga gggagcacct taaggacatc gctgagtgtg
     5401 aactgtctat gggccgttca gatctctttg agcctgacat gagggatatc taccgggacc
     5461 aatacggaga acttggtgag agccttaccg atgctctggc tagtgagccg ctaagtattg
     5521 accagcatcg ttacatcaat gaggccctga aggtgctaaa agcctgtatg aggcgtatcg
     5581 aagcgggaga tagtcagcct ctgatagatt acgtcgatcg ttttaatgat attgatcggc
     5641 aagatgacca agcagactct gtgtccctat ctgttagtgc tccagaagta aaaccagaag
     5701 ttacgcctag cattaccata gcgagcctct ttgagcaata cgaagcggag aactctcaga
     5761 actggaagcc tgcaacctta agggaaaaca aagcgtcaca cgctgcgtta atcgaaatat
     5821 tcgaccatct gggtctcaat gcagacgcta acagggctga catgcttaag gtccgtgatg
     5881 tgctccagca gttacctaag aaccgtaagc agcgctttaa ggatgcccca cttgctgacc
     5941 tgttgagccg tgaggataag acagattgtc tggatgtcgt taccatcaac aacaagtatc
     6001 ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa tgacttgatt aaaaagaaca
     6061 tgactgaggg tctggagctt aaagtccctc agcgtaaagc ctcaggggcc cgtaacgcct
     6121 tctctacgga gcaggtgggg caactactgg tcgcagctaa ggcatactct cagaagacct
     6181 ctggtaagcc ctaccactat tacgttacgg ctttggctgc tatcactggg gcgagactca
     6241 atgaaatagc ccaactacag gtcaaagacg tcaggaccac tgaggcggga accgtttata
     6301 ttcatatcaa tgaagacgat agtagtctac ccggtaagag catcaagaac gctcatagtg
     6361 accgctgtgt gcctctggtc gatggggcct atggttttat attggctgac tttatggcgc
     6421 ttgtagagac ccgtagaggc gctgacggtg atgacgctat ggtgttcgat ggtctgcgtc
     6481 tcatgaagaa cggttacggt gagcaggtga gtaaatggtt taatcggacg ttgttgccta
     6541 aagtcctcgt agaccgtagc ggcttagcgt ttcactcgtt tagacataca gttgcgaccc
     6601 agttaaaaca acatggggtc gagttggcgt atgcacaggc gatcatgggg catagttcag
     6661 ggtctattac gtacgaccgt tatgctaaag aggtcgaggt tgataggctg gtcaatgtga
     6721 tggctgatgt ttataaggag acctagagga gaccatagtc taggatgaca cttactacag
     6781 acctgtgtgg tcatgttttt gactcatagt tttctctact tatcaaggga taattaatac
     6841 gactcactat agggagaagg gacacaaaaa gttttctctt ctaggacaac taaccgtaac
     6901 ttatcgcatc ctttcagggg cgggatgacc aattacagac accaagcgtt ctacttaaag
     6961 tgaatgcttc ctgtgtgctc tgggtcagtc gataagtgaa tagcaataag acacaatcat
     7021 cttaattatg gtctctagtc cctacagggg cttaacttaa agttaactta aagacacaat
     7081 taaagattaa cttatagtat tacttaaagt taacttaaag acacaattag agattaactt
     7141 taagtattaa ataaaagatt taattaaaga ccaattaaag attaactcta agggaatacc
     7201 ttaaggggtg atgatattta taataatcaa tcaccaaacc tataggtctt ctctagggtc
     7261 tctctatagg tctctctagg gtctcctcta ggcattaccc ccagaagcta actctatggc
     7321 cctctatggt cctctatggc cctgtacgtc attctttagg atgccctgta gtttcgtata
     7381 gcctgatgcc tagagagacc ctagagtggc tcctagagag accctcccag tagctccgca
     7441 cagctacatc gtcatcatca tagtattcaa tctcaaagtc attctgtttg catcctgttg
     7501 tcctgctggt ctcccttcgc ttggggactt tgagtaggtc agtgggggtg cgacttcagg
     7561 gtgcaaacaa aaaggaaatc aagcgatgtc tcaacaacca caacaaccac aacaaccaca
     7621 acaagaaatc agcatcatta acctcgacca gcttgtgtcc atgacctcag ttgaaattgc
     7681 tgagctgaca ggcaaggagc accgaaatgt ccttcgtgat attcgtaaga tgctcgaaga
     7741 acttaac
//
LOCUS       DAJVRL010000148         7745 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVRL010000148 DAJVRL010000000
VERSION     DAJVRL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.148"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            431..2428
                     /gene="betT"
                     /locus_tag="OQN02_003596"
     CDS             431..2428
                     /gene="betT"
                     /locus_tag="OQN02_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCS1644249.1"
                     /translation="MSENDTTPKKSTSQVNKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPIGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLAVLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWALLMGVVAIALLIAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDIIQPAMTLVQEELNKQG
                     TISHISDAAEDRIRLEVDLGNELNYIYEVRLRGYNSPTFALAALDNDEQQSEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2492..3769
                     /locus_tag="OQN02_003597"
     CDS             2492..3769
                     /locus_tag="OQN02_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCS1644250.1"
                     /translation="MISRWKWMLKQTFKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAKVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYVDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSTPVLYLSQGQESSISTDLLETIIVSDVRSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSAYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPGIFAEAAQTLAFETL
                     EHATRADHIDSDRYLIKSIYYNLFSGEDSNKKP"
     gene            4386..5816
                     /locus_tag="OQN02_003598"
     CDS             4386..5816
                     /locus_tag="OQN02_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644251.1"
                     /translation="MSSELIAKPGTGVLSTDTTKFEGYLSYLGLPTENIIASLPERQI
                     IEKNLPTFIDSLPAEVKRDARYLSKFVAGAAIGLFDASLNYVWNEVVLNLREKVVVYG
                     LDMFFDAAVGGGRRELYSTEEDLSGLKDNTLINTCRKLELISDVVYTKLNHILTMRND
                     IGASHPNSYSINAFELLGWLQTCVQDILNDKPSASAIQIKSFLENLKRSTDVLDEATI
                     HSMERPLNDLSLQNTDNLLNSIFGIYTADSTGNVVRKNIALFAPHIWNKSSDNVKYKL
                     GVTLDGYKNNLHNEKHALGIEFFTFCDGNRYQSLDSRIISLDGHADDLLEARYAWDNF
                     YNEPPHMRKILSYLKTENDIPLERVHKLIKTVLICRVGKGIPYNTGVSPAGRPLYDQF
                     FGMLGDQNIINTVIAMHSNEVRVNLDNKYCQQHMVSVLTLLRANARSERIQEIIDFLI
                     ANQTILHKVHNDKRYRDLTKNHISFG"
     gene            5957..6559
                     /locus_tag="OQN02_003599"
     CDS             5957..6559
                     /locus_tag="OQN02_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1644252.1"
                     /translation="MNTSSWNKGRIVGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGSSVSSRATVLQQKTGSPVQFEITKGTREAVTALIKL
                     GNLHSNDFLFRSRVGTNQHISTRQYNRIFHGWIAKLGLDDSLYSTHSMRRTKPYLIYK
                     KTKNLRVIQLLLGHRKLESTVRYLGIEVDDALEISESIEI"
     gene            complement(6656..7465)
                     /locus_tag="OQN02_003600"
     CDS             complement(6656..7465)
                     /locus_tag="OQN02_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin"
                     /protein_id="HCS1644253.1"
                     /translation="MTKISERWKHNGITEGYCNICGKYGLLTKDHVPPKCAITLGPVL
                     QKTVSEFFGIQEPVKPLNAKNGSYFRTICSHCNNKVLGGLDVAIENVTKSFKEQLSRY
                     MNGMNVYPFIRIPFDSISFTKAMIGHVLSATSVEDCKKEPVDSPFYTPLKDYVLGKNS
                     SFEETHDIYYWFYPHRMHISAQSVAFMNEGHVAFICALHFFPIGFIITMKNEGTYPAH
                     STKLELEDKFLTFNMTSINYEYTTFPFVNLKGNQMYAISNGHTCVSYPIIK"
BASE COUNT         2154 a         1656 c         1727 g         2208 t
ORIGIN      
        1 taagaacgcg ctttaagttc atcgtcaata cgcaggttaa tgctacccat gtcttacacc
       61 tcttgtaatt acaaatgtca ttacaagtat cgcactacaa catgcttagg gcaagtcacg
      121 aaggaagtca gaaagtagtc gtaagaacgg tgatcactgt ccgctttgtg ccaagagcag
      181 acgctggtaa ttcgtgccta atatacttca cttaacttat atcccagctt tgatacagat
      241 catacaaatt aaatgcccca gtaattggca ggctgagtaa cttaaaactc cttagaatat
      301 aggatgattt gtatcggcac tcattccacc atgcattccg attaatcccg catagccagt
      361 tgaactttgc tactctgtga gaggtagtac cttctatcca gtgcggattt aattaatgga
      421 ataaatgatt atgagtgaaa atgatacaac cccaaagaag tctacaagcc aggttaacaa
      481 agcggtattc tttacatctg ctttgctaat tttccttctt gtcgcctttg ccgccgtatt
      541 cccggatgtc gccgacaaaa attttaaact acttcagcaa caaatcttca cgaatgccag
      601 ctggttctac atacttgctg tggccctgat tttactgagc gtcacgttcc ttggactctc
      661 acgttacggt gatataaagc tgggcccaga ccatgcgcag cctgatttca gctaccactc
      721 ctggttcgcg atgctttttt cggcagggat ggggatcggc ctgatgttct ttggcgttgc
      781 cgaacctgtg atgcattatc tttcgccacc tgtcggcact ccagagaccg ttgcggcagc
      841 gaaggaagcc atgcgcctga cgttcttcca ctggggtctg cacgcatggg caatttatgc
      901 catcgtggcg ctgattctgg cctttttcag ttaccgtcac ggtctgcctt taacgctgcg
      961 ttctgcgctc tatcccatta tcggcgatcg catttacggg cctataggcc acgcggttga
     1021 tatcttcgcc gtgataggca ctgtctttgg cgttgcaaca tcgctgggtt acggtgtatt
     1081 gcaggtgaat gccggtttga accatctttt cggcgtgccc atcaatgaaa cggtacaggt
     1141 cattttgatc gtggtcatca cggggttagc gacgatttcg gtggtgtccg gcctggacaa
     1201 gggaatacgc atcctgtctg agcttaatct gggcctggcg gtgttgctcc tggcgctggt
     1261 cttgtgcctg ggtcccactg tgcttctgct gaagtcattt gtggaaaata cgggtggtta
     1321 tctgtcggaa ctggtgagta aaacgttcaa cctttacgcg tatgaaccca agtcgagcaa
     1381 ctggctgggg ggctggacat tattatactg gggatggtgg ctttcatggt cgccgtttgt
     1441 aggtatgttc attgcacgcg tttcccgcgg caggacaatt cgtgagttcg tgactggcgt
     1501 gttgtttgtt cccgccggct ttacgctcat gtggatgacg gtgtttggaa acagcgccat
     1561 ctatctcatc atgaaccagg gcgccactga cctcgccaat accgttcagc aggatgttgc
     1621 gcttgccctg tttaatttcc tggagcattt cccgttctct tccgtgctgt cattcattgc
     1681 aatggcgatg gtcatcgtct tctttgtaac gtctgctgat tcgggggcaa tggttgtgga
     1741 tactctggca tcaggtgggg tggcaaacac gcccgtctgg cagcgaattt tctgggcatt
     1801 gctcatgggc gttgttgcta ttgccctgct gatcgcaggg gggctgagtg cgttgcaaac
     1861 cgtaacgata gcgagtgcat tgcccttctc agttatctta ttaatatcca tatacggact
     1921 gttaaaagcc ctgcgtcggg atttgaccaa gcgtgaaagc cttagcatgg cgactattgc
     1981 tcctacggct gcacgtaacc caattccctg gcagagaagg ttacgcaata tcgcgtattt
     2041 gccgaagcgc tctcttgtga aacgttttat ggacgacatt atccaacccg ccatgacgct
     2101 ggttcaggag gaacttaaca agcagggaac gataagccac atcagtgacg ccgccgagga
     2161 tcgtattcgc cttgaagtcg atttgggcaa cgagctgaat tatatatatg aagtgaggct
     2221 tcgcgggtat aactcaccga ccttcgcgct cgctgcgctg gataatgatg agcagcagag
     2281 tgaacaacat cgatattatc gcgctgaagt ctatctcaaa gagggcggcc aaaattatga
     2341 tgtaatgggc tggaatcagg aacagctgat taatgacata ctggaccagt acgaaaaaca
     2401 cctgcacttc ctgcatctgg ttcgttaaca gcaacatgcc gccacagagg cggcatttat
     2461 ttatcccggc tgcaatatga gggaatgcag aatgatttca cgctggaaat ggatgctgaa
     2521 gcagacattt aaaaaactat ggttcagggc aacgttattc gcaattgtcg caataataac
     2581 ggccctttta tcaattcttt ttaaatcaat gatacctgag tcggtttccg tgaaggttgg
     2641 tgcggaagca gtcgataaca ttctgaacat actggcatcg agtatgctgg cagtgaccac
     2701 attttcgctg agcatcatgg tcacagccta cggttcagcc actactaatg tgactcccag
     2761 agctactcgt ttagttgttg aagacgtcac cacacaaaat gtactggcca ccttcatcgg
     2821 ttcttttctc ttcagtctgg tagggattat tgccctcaat atgggagctt atggagaaag
     2881 ggggagagtc attttattca ttgtaacact ggttgtcatt gccttaatcc tcatcacatt
     2941 gcttcgctgg atacagcatt tgacctctct ggggagggtt ggtgagacaa cggcaaaagt
     3001 agaacaggcg gccatcgaaa catttattgc gagagcaaga aatccctgtc tcggcggata
     3061 tccatggctt gagaacaatg aacagccgaa aggaacggtt gcagtttatc cgaaaaagat
     3121 tggctatgtt gaatatgttg atatggtgaa actcagcaag ctgctgacca atgatccccg
     3181 tcatgtatac ctcgtggcgc agccgggcag tttcatacat ccgtccacgc cagttttgta
     3241 cctgagtcag ggccaggagt catcaatcag caccgattta cttgagacga ttattgtctc
     3301 ggatgtacgt tcatttgctc aggacccccg attttgtctt agcgtcatgg ccgaaatagc
     3361 ctgccgagcc ctttcccccg cagtgaacga tcctggaacc gccattgatg tcattggcag
     3421 aggtgtacgt atactttccg cttacgcgca gaataaatct gatgaaatag aagtgaaata
     3481 tccttctgta catgttgcac cacttcagaa taacgatcta cttgaagact ttttctcacc
     3541 tgttgcgcgc gatggtgcca gtatgaggga aattcagata agggtcctta aaggactgtc
     3601 gatgctgagt aagggttggc ctggaatttt tgctgaagcc gcacaaaccc tagcatttga
     3661 aacattagag catgctactc gtgctgacca tatagattct gacagatatc taataaaatc
     3721 aatttattat aatttattta gtggcgaaga ttctaataaa aaaccatagt tgcggagcaa
     3781 gcgatacggt tgttggcaag tcaagggcaa agcattcgcg tgagagtggg cgtctgcttt
     3841 gtgccaaaag cgggcattgg gcgttgtggt aaatgcctga taatttcttc ttttactagc
     3901 acaagccgct ggataattcc catgtgtcca gcttaagtaa atcaagaaca actcgcgtac
     3961 tggctgtata aagtttgccg gacgcaaatt tattgatttc agaaaaacct gttgaaatag
     4021 aggtatatca gcgttagttt gatgtgagta aagatcagga aagaaggcag aaaaatgttc
     4081 cacttgcggg caaaaaagcc ttctattaaa ttgttaaggc tttatgaaga tcgtacatta
     4141 cgaatcgaat tcaccatggg tagggaacat gaaatgtcca aaccctaagt gcgggagaaa
     4201 tgactcctgc ttgattcggg ataggttgta ctcgcatgaa gtatgtattg gatctaaacc
     4261 caaatactgg tggcgtttgc tcacaaatac cttaacaagt ttaggtatgg gacgctccta
     4321 acgtcgcgcc cctgctaaaa gcgttagctg ttctctagcc ttcaaccaga taaggaaata
     4381 atcgtatgag ttcagaattg attgcaaaac ctggaacagg ggtactttcc acagatacaa
     4441 caaaatttga aggatattta tcctatcttg gtttgccgac cgaaaatata attgcctctc
     4501 tccctgagcg ccaaattatt gaaaaaaacc tccccacatt catagattct ttacctgctg
     4561 aggtaaaaag agatgctcga tatttatcaa agttcgttgc tggtgcggct ataggactat
     4621 ttgatgcatc gttaaattat gtttggaatg aagttgtact gaatttaagg gaaaaagtag
     4681 tagtttatgg cctcgatatg ttctttgatg ctgcagttgg aggaggacgt agagagcttt
     4741 attctaccga agaagatctt tctggtttaa aagacaacac ccttattaat acttgccgaa
     4801 aacttgaatt aatatctgac gttgtttaca ccaagctcaa tcatatattg actatgagga
     4861 atgacattgg tgcttcgcac ccaaattcat actccataaa tgcttttgaa cttcttggct
     4921 ggctccaaac atgcgttcaa gatattttga acgacaaacc gtccgcgtct gccatacaaa
     4981 taaaatcatt ccttgagaat ctaaaaagat ccactgatgt tcttgatgaa gccacaattc
     5041 atagcatgga aagacctttg aatgatctgt cgcttcagaa tactgacaat ctactcaata
     5101 gtattttcgg catatataca gctgattcaa caggaaatgt tgtcagaaaa aatatcgctc
     5161 tttttgctcc gcatatctgg aataaaagca gtgataacgt aaaatacaag ctcggcgtga
     5221 cgctagacgg atacaagaac aatcttcaca acgagaaaca tgctttaggt attgagtttt
     5281 ttacgttttg tgatggtaat cgttatcaat cattagactc tagaattata tcactggacg
     5341 gccatgccga cgatctctta gaggctaggt atgcttggga taacttttat aatgaacccc
     5401 ctcatatgcg gaagattctt tcgtatttaa agactgaaaa tgacatacct ttagagaggg
     5461 ttcataagct tataaaaaca gttttgattt gtcgtgttgg taaaggaata ccatataata
     5521 ctggtgtttc tccggcggga cgacctttgt atgatcagtt ttttggcatg cttggcgatc
     5581 aaaacattat caatacagtt atagcaatgc actctaatga agtgcgagtg aatttagaca
     5641 acaaatactg ccagcaacat atggtaagcg ttttgacttt gttaagagcc aatgctcgta
     5701 gcgaaagaat tcaagaaatt atagattttt tgatcgccaa ccaaacgatc ctccataaag
     5761 tacacaacga taagagatat agagatctaa ctaaaaatca catcagtttt ggctaacaaa
     5821 gcgctgcaca cggactaaat taagcttcgc tcactgggac gggctacagc ccgtccctta
     5881 accaaacgtt aatgtctgca attcgctcat aagggacaac catactcaaa tctcccacat
     5941 tgcaggagat ttgagtatga acacttcatc gtggaacaaa ggccgtatcg taggccaaaa
     6001 aagaccactt cagatatctc atatctgggg gatccgaatc aggcttgaac tggaaggtaa
     6061 gacgcgcgat ttggctctgt tcaatatggc cctggacagt aagctacgag gctgcgatct
     6121 ggtaaaactt aaagtatctg atgttgcata tggtagctct gtttcaagca gggcaacggt
     6181 gttgcagcag aaaaccggta gccctgtcca atttgagata accaaaggga caagagaagc
     6241 tgttactgca ttgataaaac ttggtaattt acatagtaat gacttcttgt ttcgatcccg
     6301 ggtcggtact aaccagcaca tatcaacccg gcaatacaac cggatttttc atgggtggat
     6361 agcaaagctt ggtctcgacg attcgcttta cagcacacat tccatgagaa gaacaaaacc
     6421 ttaccttatc tacaagaaaa ctaagaatct cagggtgatc caacttctgt taggccatag
     6481 gaaactggaa agcacagtcc gttatctggg cattgaagtc gatgatgcgt tagagatctc
     6541 tgaatcgatt gaaatctaag gttgtcaggg ctgcaatagc agccctgtgc caacagcgga
     6601 cattatagat ggtatatgta gaaagtgaac atgcgtagat tgtatgattt agccgtcact
     6661 taattattgg ataactcaca caagtatgac cgttgcttat cgcatacatt tggtttccct
     6721 taagatttac aaatggaaat gtcgtatact cataatttat agaggtcata ttaaatgtaa
     6781 ggaatttatc ttcaagttct aattttgtag agtgggcagg gtatgttcct tcatttttca
     6841 ttgtgataat aaaaccgata ggaaaaaaat gtaaagcaca aataaatgcc acatgtcctt
     6901 cgttcataaa tgctacactt tgagcggata tgtgcattct gtgtgggtaa aaccagtagt
     6961 aaatatcatg agtttcctca aaacttgaat ttttacccaa aacataatcc tttaaaggtg
     7021 tatagaaggg gctatctacg ggttcttttt tacaatcttc aactgaagtt gctgatagca
     7081 cgtgcccaat catagcctta gtgaaagata tgctatcaaa aggtattcta atgaatggat
     7141 atacattcat accattcata tatcgactta actgttcttt aaaggacttt gttacatttt
     7201 caattgcaac atcgagccca cctaatacct tattgttaca gtggctgcaa atggttctga
     7261 aataagagcc atttttagca tttaatggtt taactggttc ctgaatacca aaaaattcgc
     7321 taactgtttt ctgcaaaaca gggcctaagg ttatggcgca ctttggagga acatgatctt
     7381 ttgtgagcaa gccatatttc ccacaaatat tgcaataacc ttcagtaatg ccattgtgct
     7441 tccatcgttc actaattttc gtcattttta ttccctcagt agcataagct atctatacgt
     7501 tttttaatcc tatcaaagat acaacttaac gtcaggaaaa tgatagtgtt tatctaagta
     7561 atacaattaa cttcaacgcg tgacagtctg cttttcgctc ttagcggaca ttagcatagg
     7621 ctatttacca taacgcctca ttacgcgtac cgctcagacg gcctgagcgc gtttctgacg
     7681 tatccccgtt aaaaacagta acaaaccacc cgaaaatgca cgccagaaac gctacgtaag
     7741 aatct
//
LOCUS       DAJVRL010000149         7743 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVRL010000149 DAJVRL010000000
VERSION     DAJVRL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.149"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="OQN02_003601"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="OQN02_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCS1644254.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="OQN02_003602"
     CDS             1102..2562
                     /locus_tag="OQN02_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1644255.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OQN02_003603"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="OQN02_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1644256.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OQN02_003604"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="OQN02_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS1644257.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OQN02_003605"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="OQN02_003605"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1644258.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="OQN02_003606"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="OQN02_003606"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1644259.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="OQN02_003607"
     CDS             complement(6727..7245)
                     /locus_tag="OQN02_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS1644260.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7743
                     /gene="ydeI"
                     /locus_tag="OQN02_003608"
     CDS             7521..>7743
                     /gene="ydeI"
                     /locus_tag="OQN02_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCS1644261.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1940 a         1812 c         1955 g         2036 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctagcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc tccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcag
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aag
//
LOCUS       DAJVRL010000150         7728 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVRL010000150 DAJVRL010000000
VERSION     DAJVRL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.150"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..835)
                     /locus_tag="OQN02_003609"
     CDS             complement(<1..835)
                     /locus_tag="OQN02_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCS1644262.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQ"
     gene            1017..2441
                     /gene="sbcB"
                     /locus_tag="OQN02_003610"
     CDS             1017..2441
                     /gene="sbcB"
                     /locus_tag="OQN02_003610"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1644263.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2484..2711)
                     /locus_tag="OQN02_003611"
     CDS             complement(2484..2711)
                     /locus_tag="OQN02_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1644264.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2725..3783)
                     /gene="yeeE"
                     /locus_tag="OQN02_003612"
     CDS             complement(2725..3783)
                     /gene="yeeE"
                     /locus_tag="OQN02_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1644265.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3962..5320)
                     /gene="plaP"
                     /locus_tag="OQN02_003613"
     CDS             complement(3962..5320)
                     /gene="plaP"
                     /locus_tag="OQN02_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1644266.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5310..5372)
                     /gene="yoeI"
                     /locus_tag="OQN02_003614"
     CDS             complement(5310..5372)
                     /gene="yoeI"
                     /locus_tag="OQN02_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1644267.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5533..6516)
                     /locus_tag="OQN02_003615"
     CDS             complement(5533..6516)
                     /locus_tag="OQN02_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1644268.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6562..7386)
                     /locus_tag="OQN02_003616"
     CDS             complement(6562..7386)
                     /locus_tag="OQN02_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1644269.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7469..7723)
                     /gene="yoeB"
                     /locus_tag="OQN02_003617"
     CDS             complement(7469..7723)
                     /gene="yoeB"
                     /locus_tag="OQN02_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCS1644270.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2069 a         2013 c         1812 g         1834 t
ORIGIN      
        1 tttgttcgaa gattggacgg cggataacat tacccatctt gctatgctct ttcggtacgg
       61 tccatacgtt gtccagcagg tcaaattctg tctttgttgc cagcctaagc tctgagagcc
      121 tcgcccccca cagcataagc atctgatgaa ggagcttatt tgacgtagac gcacggcttc
      181 tttcaatagc aagccaaatc ttagccagtt cgtgatacga cagtacccga tcccctacct
      241 cagcgcggga gccgaagtcc cttggctgga tgctcataat tgcgcaacta tctatcaact
      301 gacgccgcat gcaccaacta attgctgatc ttagttgact tagcacctgc cttgctcggc
      361 gtggattatc tttttcttct tcggtaagca ggtcaaccca ttgcttaacc gtgatagaag
      421 atgccggacg attagggaag gcgtcatgca tgcgcttcat aaccgttgat cgataaagtg
      481 cctgggtctt ttctctgaga gttgtagaga cgtagttgtc gaaccagtag tcgagacact
      541 gggcgaccgt catggagttc tccaccttct cttcaaaata ggtgcgtgga tccgttcctg
      601 agaaatagag ctttcgcaag tcagcagtga tctgtctggc atccttcaaa gacagggatg
      661 ggtatcgacc aagcccaagt cgattaggct tgccatgcca acgatagcgg tactggaact
      721 ggatgacccc cttcggtgaa attcgtacgc tgaggccatc ggcatcagcc acttcttgtg
      781 ggcccgaata tggtttacca taaatagtac gcagttttgt gtcgcttata gccataaata
      841 atatttggta cgctcagaaa taatagtttg gcacacatct ggtacacaat atcacatgga
      901 caacagcgca cagtaaacaa ctatatttga cgttcgtaga catactcagg tgaaaaaagg
      961 gttgcttttc gagttataac agacagttaa ttaactacag tggacaatgc tagacaatga
     1021 cagacacaga taaacaacct accttcctct ttcacgatta cgaaaccttt ggcacgcacc
     1081 ccgcgttaga tcgccctgca cagttcgcag ccattcgcac cgatgacgaa ttcaatgtca
     1141 tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cggcctggag
     1201 cagtattaat taccggtatt accccgcagg aggcacgggc gaaaggagaa aacgaagccg
     1261 cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca
     1321 acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc
     1381 cttacgcctg gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg
     1441 cctgttatgc cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga
     1501 gctttcgcct tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg
     1561 cgatggctga tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcagccac
     1621 gcctgtttga ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg
     1681 ttccgcagat gaaacccctg gctcacgttt ccggaatgtt tggggcatgg cgtggcaata
     1741 ccagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attatggcgg
     1801 atttggcagg agacatttcg ccattactgg agctggatat cgacacattg cgcgagcgtt
     1861 tatataccgc aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtgcata
     1921 tcaataaatg tccggtactg gcccaggcga atacgctacg cccggaagat gccgaccgac
     1981 tgggaattaa tcgtcagcat tgcctcgata acctaaaaat tctgcgtgaa aatccgcaag
     2041 tgcgtgaaaa agtggtagcg atattcgcgg aagccgaacc gtttacacct tcagataatg
     2101 tggatgcaca gctttataat ggctttttca gtgacgccga tcgtgcagca atgaaaattg
     2161 tgctggaaac cgagccgcgt aatttaccgg cactggatat cacttttgtt gataaacgaa
     2221 ttgaaaagtt gttgttcaat tatcgggcac gcaacttccc ggggacgctg gattatgccg
     2281 agcagcaacg ctggctggag caccgccacc aggtcttcac gccagagttt ttgcagggtt
     2341 atgctgatga attgcagatg ctggtacaac aatatgccga tgacaaagag aaagtggcgc
     2401 tgttaaaagc actttggcag tacgcggaag agatcgtcta atttgaagcc gccgccgctg
     2461 gtacggcgga ggacaatgtt gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg
     2521 ccaatctgct gataatcggt gatggcatgt ccttcttctg ccgcccactg tgggattgct
     2581 tcagttgcct gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc
     2641 agtgctgctt ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt
     2701 ttaatcgcca tctttacacc ttacttaatt tgccgcagca gttgccagtc gcgccttacg
     2761 ctgcggtcga acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag
     2821 gccaatccag ccctgccagg tcatcattgc agtcataacc agtccattac cgatagagca
     2881 accacctgca atactggcac cgaagcccat cagtacacca cctaacccgc tacgtaatgt
     2941 tgtttgtgca tcagctgcgc gaacgcggaa ctcacggctc gctttggctg caataaatgc
     3001 ctccacgaag atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc
     3061 gaccagaaat tgaagaatat tggccgttgg ggaagtgact cctaacccaa acatgcgtcc
     3121 ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagcagtgac
     3181 aaagggatgc cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa
     3241 ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac
     3301 cagcaaaacg gtaaccaacg gccacacaga caaattaaaa gtctcagcaa tagagttatg
     3361 ttcagtagtg tagtgctgca aggtttgatt taaaccactg gcgtgtggag aacgcatcac
     3421 cgcactcatc accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc
     3481 gcgataccag gtcccggtgg cacatccacc cgccagaaca attcccagcc cgaaaagata
     3541 cccacctata acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc
     3601 ctgaattaac gcaaaaaccc ccacgctttg tgccgaaatt gcaatcagca aggcgtaaaa
     3661 catgcgatta tttttcgcga tatacatatc gcgaaaacca ccagtcaggc aaaaacgccc
     3721 acgctgcatg acaaatccca gcagagcacc acaaattagc ccgcttaata tcattgaaaa
     3781 cataaattaa ttaaccagat gaatgttaat ggggaaattt ttcatgactg gtggaatgca
     3841 gaccaataac caaattctct aaattagaac aaatggttat taatgaggat ttcgataaaa
     3901 aagaataaaa aaaccggagc caaatgttca ttcgactccg gtttattatt agaaaagatg
     3961 attatgctac gtcttcgtac tgcggaaccg gattacggaa gctcttggtg acgcaagcca
     4021 ggtaaatcag accgatagca gcccagatca gacccagaac cattgagctt tcttccaggt
     4081 taacccacag cgcaccaacg gtcagcgcac cacacatcgg caggaacaga tactggaagt
     4141 gatctttcag cgtcttgtta cgcttctcac ggatccagaa ctgcgagatg accgacaggt
     4201 taacaaaggt gaacgccacc agcgcaccaa agttaatcag cgccgttgcc attaccaggt
     4261 cgaagttgat tgccagcagg gcaatcgcgc caaccaggat gatgttcatc gccggagtac
     4321 gccatttcgg gtgtacataa ccgaagaagc ttttcgggaa cacgccgtca cgacccatta
     4381 cgtacatcag acgcgctacg cctgcgtgcg ccgccatacc ggacgccagt acggtaatgg
     4441 tggagaagat cagcgcgcca acctggaacg ctttacccgc aacgtacagc atgatttcag
     4501 gctgtgacgc atccggatct ttaaagcgag agatatccgg gaagtacagc tgcaggaagt
     4561 aagttgcaaa gatgaagatc atgccgccaa tcagcgcggt caggaaaatc gcacgcggga
     4621 tcacgcgctc tgcatctttg gtttcttccg acagattgct gatgccgtca aagccggtaa
     4681 aggagaagca caggattgtc gccccggtaa tcatcgggat aacatgcgca tcgccagacc
     4741 agaacggacg cgtgctcgcc agcgtaccag cgccttcacc ttcaaatacg ccataaacaa
     4801 ccatgcccag aatcaccgcg atcagcacta cctgcaacac gacgattaca gtgttgacgt
     4861 tcgctacgga tttcagacta cgcaggttaa aggcggtcat aaaggccacc agcgccacca
     4921 caaacatcca cgatggaatg gaaggcacca gagcttcaaa atagattttc gccagcagaa
     4981 tgttgatcat cggcgcgaac agatagtcga gcagagaaga ccaacccacc ataaagccga
     5041 cagtcgggct aatggatttc tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc
     5101 gaaccagctt cccgtagctc agagccgtaa acaggatcgc aatcaatgcg aacgcatagg
     5161 ctgtcggcac atgaccatcc gtgaggcctg aaacgatacc aaatgtatca aacagcgtca
     5221 tcggctgcat ataggctaga cccatcatta caaccggaac taacgtaagc gttttacgca
     5281 attccacgcg agaggtgttt ggagtaacgt tatgcgacat ggttattctc ctttacggtg
     5341 ataaccgtcg cgtaagcaaa aaattgcccc atttttttgg attcctcagc gacaacaact
     5401 gtcgattttt agtaaatatc tatccggtac gaagcccggc ctcttggtat gaatgattgg
     5461 tttgaagcaa aaaataaccg acgctaatta aacgtcggtt tttaatcatt ttttgacagc
     5521 ggcgcattgt gcctaaatgg gggggaaatg acaagagaat gagaggcttg tcataataat
     5581 ttttctttga atggctgatt ttccgtcatc agattttgca taaacaccgg cgatcgcact
     5641 atttgctaaa atttcatctc gccaccagcc gctggcaagc cctgcggtct gttcattcca
     5701 gccaatccag acaggttcaa agagagtttg ggcgaccact ttcttctcga ttaacgcgcc
     5761 actatcgaga aaacgttgcg ccagataccg tggtaaatag ccgcagccaa ggccgctaat
     5821 ttgcagctcc agtttggttt taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag
     5881 ttgtgaggcc gtagaagcgc ctgcctgcgc agtgtccccc accacaatcg cccgatatcg
     5941 tttaatgata cgacggttta acggttcttc ttcctgggct aacggatgat gaggcgcgac
     6001 cgcaaagact tgttcaagat cgcccaaccg cgagaaacca aactcactgg aagatggtgg
     6061 ctcatgcatc gcgccaacga taatatccgc tcgcccctga gtcagggcat cccaggaacc
     6121 accgagtacg ccattgataa acttcaggcg cgtcacgcta tgatgttgat aaaaagcttc
     6181 aatgagaggc gcaagcagag aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt
     6241 ttcccagcct tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg tatgcagaac
     6301 ttcccgccct ttctctaata acatttttcc ggtgcgggtg aatttagcgc ggtgtccgct
     6361 acgatcaagc aattgaatat ttaggtcgct ttccagcttg tgaacggtgt aactcagagc
     6421 ggacggcgtc ttgtagagtt tggctgacgc cgccgcaaaa ctgccctctt tttctatggc
     6481 atcgaggatc atcaacacat ccagcagtgg tttcatacac gcccccttgc agtgcatgcg
     6541 gcgtcctgct ggcgtgatcg tttactccaa cggcattacc agcggatcgg gatactggta
     6601 ttcaaatccc agttcattac aaatccgact gccatcaata atcttacctt tgccgctgtc
     6661 cagactattt ctgaactgcg gaggttgcag ccccagtaaa cgggccatct gcggatagaa
     6721 aacattacgc gcagggtgag ctggcgcaca tatattatag atgtgtccgc ctttaggtgc
     6781 ctgtaacaac agagtgatag cgccaataac atcttctaaa tggactaaat taacaccatg
     6841 ttcaccatca ggggcggttt ttccggcaaa gaagcgtccc ggatgacgtc ccggtccgac
     6901 caggcccgca agacgcagaa tatcgaccga agtaccgggt aaattgtgca gccagtcttc
     6961 gagttcttct aatactcgtc cactgttggt taccggatta cgcggggtag tttctttaac
     7021 cgtgccttgc gcgtcgccat agacagatgt tgagctggta aaaataattc ggggaatacg
     7081 atgggccagc gcgctatcca ctaactcttg taccgcttgt aaatagaact catcgccggg
     7141 gccgctacga cgtgccggaa gcgtaatgac cagcgcatcg gcatccataa gggcatccag
     7201 atcgtcagaa tcgcaaacta actcaggctc catgcgaagc agatagctat caatgccact
     7261 cattcgggcc gcttcgacac catcttgtgt ggttttactc ccggtgactt gccagcctcg
     7321 cgctgaaagt gacatcgcca gcggcatacc taaccaccct aacccgacaa ttgcgacctt
     7381 tttcatccgt tatctcctga tacaccttag atctataagg ctacgctagc gtatcaaaac
     7441 tgacaattca ttctatgaat gaatctgttc aataatgata acgacatgct gcaatgagca
     7501 gtgaatcatc agtaaccgcg tataccagac ggtgctcctc tgtaatgcgt cgggaccaga
     7561 aacctgacaa attatgtttc aggggttctg gtttcccctt accttcaaat ggtgttctgc
     7621 gggtatcttt gataagttca ttgatctttt taacaattcg cttatctgtt tcctgccagt
     7681 acagataatc atcccatgat tcctcagacc agattagttt cactcaat
//
LOCUS       DAJVRL010000151         7554 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVRL010000151 DAJVRL010000000
VERSION     DAJVRL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.151"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OQN02_003618"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OQN02_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OQN02_003619"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OQN02_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1644271.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OQN02_003620"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OQN02_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OQN02_003621"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OQN02_003621"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1644272.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OQN02_003622"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OQN02_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1644273.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OQN02_003623"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OQN02_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1644274.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OQN02_003624"
     CDS             complement(6510..7202)
                     /locus_tag="OQN02_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1644275.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1922 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgactatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgt aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcgcact gctctttaac aatt
//
LOCUS       DAJVRL010000152         7216 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVRL010000152 DAJVRL010000000
VERSION     DAJVRL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.152"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(220..600)
                     /locus_tag="OQN02_003625"
     CDS             complement(220..600)
                     /locus_tag="OQN02_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1644276.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(593..793)
                     /locus_tag="OQN02_003626"
     CDS             complement(593..793)
                     /locus_tag="OQN02_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCS1644277.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(888..1145)
                     /locus_tag="OQN02_003627"
     CDS             complement(888..1145)
                     /locus_tag="OQN02_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644278.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(1142..2533)
                     /locus_tag="OQN02_003628"
     CDS             complement(1142..2533)
                     /locus_tag="OQN02_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1644279.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(2530..3408)
                     /locus_tag="OQN02_003629"
     CDS             complement(2530..3408)
                     /locus_tag="OQN02_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1644280.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(3419..4327)
                     /locus_tag="OQN02_003630"
     CDS             complement(3419..4327)
                     /locus_tag="OQN02_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1644281.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(4314..4547)
                     /locus_tag="OQN02_003631"
     CDS             complement(4314..4547)
                     /locus_tag="OQN02_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644282.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(4544..5560)
                     /locus_tag="OQN02_003632"
                     /pseudo
     CDS             complement(4544..5560)
                     /locus_tag="OQN02_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            5492..5755
                     /locus_tag="OQN02_003633"
     CDS             5492..5755
                     /locus_tag="OQN02_003633"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1644283.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            5745..6053
                     /locus_tag="OQN02_003634"
     CDS             5745..6053
                     /locus_tag="OQN02_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1644284.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(6095..6271)
                     /locus_tag="OQN02_003635"
     CDS             complement(6095..6271)
                     /locus_tag="OQN02_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCS1644285.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(6268..6678)
                     /locus_tag="OQN02_003636"
     CDS             complement(6268..6678)
                     /locus_tag="OQN02_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCS1644286.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(6675..6953)
                     /locus_tag="OQN02_003637"
     CDS             complement(6675..6953)
                     /locus_tag="OQN02_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644287.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(7028..>7216)
                     /locus_tag="OQN02_003638"
     CDS             complement(7028..>7216)
                     /locus_tag="OQN02_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644288.1"
                     /translation="ASVLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIAN
                     MPKSEYDEAWRQENVTEY"
BASE COUNT         1728 a         1952 c         1650 g         1886 t
ORIGIN      
        1 accaactgag ctaaagggcc ggaggcagaa taataaccat atgtcatcac atctgcaaac
       61 tcatctgacc accagcgtgt ttaacgtcct gtaccgtttt tcaggcataa aaaacccgcc
      121 cggaggcggg tttaagctgt gtggcgaagc aaccactctt aacagaatat cccgtttttt
      181 acgtacgtaa aatattttct agaaagtcgc cccttaccat cagggatgtt caatatattt
      241 gtccatttct aaccggacac ccaacatcat caacattcct tccaccaccc cctcggcttt
      301 ttgtagcttc tttcctatat aaccatctga gcatccatgc ttccgcgcca gcgtcatgaa
      361 tgtcatccca aacacgtagt agtcaaccag caagtcatgt agatcgttgt tgttccggtt
      421 aaggcgggcc atacacccac atataaccat cgcgtcatcg tcacagcatt gcgggcggga
      481 ttttactttt gaagggatta atcccttaaa accggcagca atggacgacc aggtcacatc
      541 ctcatgatta ttagccaccc acgcgcccca gcgctcaaga acctgctgaa tatcacgcat
      601 cactgtcttt acccctgtcc catccacgat gaacaatcag aacaccatca acaatggcgt
      661 gccctttgcc ctctttatct tcagcatatt ttcttaccgt ggcgcgatta cagttcagca
      721 ttcgtgaaac ttcggtcata ttacctcgtg tctggataag cagttccggt atcgtttgaa
      781 ttttgacgct catcaaatac tctccggttc ggtgattttt atcccgggct ttccaccagg
      841 gacataatga tcgcgcacaa gccagggcaa aacaaactca tggcgcatca gcgcagcgcc
      901 tcctgcacca gtttttcaaa ctttccgact ctggtttcca gctctgccac acagtccacc
      961 agctcatcta ctgctttctg tgcgcgatgc ttcgcctgca tcagttcccg aagcgcgggt
     1021 accatatctt tacggatagc atcttttgtt atgcccgttt tttcgagttg ttcagcatga
     1081 cgcagcattt cctgcgcgtg tttacgcaat tgttcagggg taaaagtcat tgtctggttg
     1141 ttcaaaagaa acgctccatc ttactgctgt cagttcgttt attactgtat ctgcgcggat
     1201 tgccgggctt catgggagtg gaaagcaccc gtgcactttc ctggtccaca ggcagaaaat
     1261 gtccgttata aaaacgccgg taaatcgttc ccagagaacc gttacgttgt ttcgtgatat
     1321 tgatttctgc gatgcccctg gcctgcgtat ccgggttgta cacttcatcc ctgtaaagca
     1381 tcagaatgat gtctgcatcc gcctctattt ctccggaatt tttcaggtct gagttcatgg
     1441 gacgtttatt gggtctggac tccacaccgc gggagagctg gctcagcgca atcaacggaa
     1501 aaccaccgga ttttgccagg cctttaagcc cctttgagat ttcacccacg gcaaggtcat
     1561 gacgccccgt ggttcgggtt tttatcagcc cgagataatc aaccaccacc agcgccgttt
     1621 ccggatattt aatcaggtgg tgtttcgttg ttgcgcatat ctcatcaatg gccaggttcg
     1681 cctggtccac catccagata ttgcgcccgg tcatccgccc caccccttgt gagaaacgcg
     1741 tccagtcttc atcttcaaag tgagccacag atttcaggcg tgatactggc atccctccag
     1801 ccgcagacac catacgttca ccaatctgga tgttcgccat ctccatggtg aacagaagca
     1861 caccatgccc ctgctcagtc accttgtcga tgatgtccag cgcaagttcg gttttcccca
     1921 tcgaaggacg ggcggcaatg aataccaggt ctccgggctc cataccgcct gtttttgcgt
     1981 ccagttcatc aataccggtc atcaacgtcc tggatttctc cagcccctga ttccggcatt
     2041 caacacgctc aaccacttcc ggaagcacat catcaatatg taccggctga atgacgccct
     2101 ttcctgtcga cagtgtgacc atcatgttct gcgcatcctt cagggcatct tcagctgctt
     2161 cacaggtatg cgcatcacgt aatttctgca gcgcctcatt cagtgttttt tctgcatcgc
     2221 gcagtgcggc attgcgccgc aacgctgcaa catagtgctc cagtgaagac ttcacccagg
     2281 ttttacgccc ggtatcagta atcaccgggg caagttccgg catctcatta cacaacagca
     2341 cggggtcaat cactcctgaa acacgggcct gtctgcagat gcctgtatag atatcctgat
     2401 acgctcgtac agaaaaaacg tccgctggca gtgtggccag aatatccatc acttcaggat
     2461 ctgctccgcg cagaaagaac gcgccgatga cagcgccttc aaggtcatcg ttacgccata
     2521 ctggagttgt catgcagcca cacctctgat acgagaacgg taactgggcc agttaaacga
     2581 caaccagttg cgtcccccgt ctgtgatcct gtcggcaatt cggggactga tgaacgccca
     2641 caactcttcc ggtgagaggt tactgataag aatggtgggc aagatacttt cgtacctggc
     2701 gttgataatt tcctgcaaaa tagccatttc agccgcgctg ccaaactgaa cgccaacttc
     2761 gtcgatgatc agcaaatcca ttgacgcata acgctcaata acttcatccg ctgttttttc
     2821 gctgtcattc cgccagcaat ttttcacagc acgggtaagg cgcatcacgt cggtgatctc
     2881 cacactggcc agatagttac ggatgatgtg ttttgccata gccacagcca gatgattttt
     2941 tccggtacca caactgccgg tcataacaag actggtaccg ttctccagca tatctggcca
     3001 gttctccgca tagcggcgac aggccgcaag atttctggct gcgccaggat taacctccag
     3061 ataattatca aactcgcagt cccgaaaacg cagggcaatt ccggcgttat cagtcagctc
     3121 ttccgccttg atggacgaca gctccatggt caaatcgttg gcctcagcga ttaagcagtc
     3181 agggcagcat gaaatttctt ctctgtcctc gccattacga tcgctccaca ccagtatatg
     3241 cgtgtgatat tcgccatgtt tttcgcaata tccgcgacct tcacgcatca ggcaggaacg
     3301 ataaggccat ggcttttcgc ccttctgagc aaatgcaatc tctgcccgta actcatccat
     3361 tcgcgcctgt agtctttttt gttgttcacg caggttaaac gtcatcatcg ctgtcacctc
     3421 agaatgtcag tttgtcactg gatttaccga atttgtcaga catggctccc aggccagcca
     3481 ggacatcgac ctgtcgctgt cgcccacctc tgggagcggc tggctgttgc cagaagtctt
     3541 cgaagtgacg atcgggtcca aagaacgtcg cagcctgctt cacgaactgt gtgccggtat
     3601 ttcctgtggc acgtacccag gcagcatagc gtttcacgcc atcaagcatg gcttcaggtt
     3661 ttattccctc cctgagccgg gctttccagg ctttgaaggc tgcggatttt gagttaccac
     3721 cagcacgttt tggatattcc tgccaggcct gttcaaattc cggtgaatat tcctgtcggg
     3781 aagaacgcgc tggtgcagac gcgtcagcgg atgcgccaat agtgttttta ctctctgtag
     3841 tattctctga agtaatctct gttgtattct ctgtaagatc gaaattggtt ttcccttctc
     3901 cgcggcgagg ggtttcccgt gtccgcggtg aaggctttcc ctcctccgcg aaattgggtt
     3961 ttacagtttc ccgaaaacgg gtttccccat ttcgggaaaa ctgattgttt tcattgataa
     4021 tttcattaag gcgctcacaa tctatacggt agaacatttt gtgctcaaga cgcttgttgg
     4081 tttcaaccaa aatgcctctg gacacaagat gcttacgcgc tacagcctgt tgttcaaatg
     4141 taagtccggt ttcgtgttgt atctcttcac gcgttttatg tacgccttcc gctgcatgtg
     4201 ctttatcctg ccagtaaaaa atctgaccaa agaaaataac agcgtgcgga cttcccatgt
     4261 atttaacgag cccagggtaa taagcaaccg gatgtccaaa atcgagcaga agatcagacg
     4321 gacgcatagc cacctcccag gcgtttaaac atttttccgg gctgaaacgc caccagcgga
     4381 taactcaggg tatgagtacg tccctgaacc tggcagacaa ccttctggct ttcggtactg
     4441 accaggcaaa cccgcagaac gtagccgttg ctggtggtga accactgccc cacacggggg
     4501 caatggttgt atcggtgata caggaaatta acgatgtggc ggatcatggg cgcacctcct
     4561 tgtcagaacc attcagcctg gaatcaacaa gtgcagcacc aaaaacagca tcacctacac
     4621 ggtcgtacag tttgccagcc agcggagatt caacggcctt aagcattggg taaagctggc
     4681 ttgtccagat ttgatggatt tcacgcaaat gcaggtatac gcctctggcg ttttgtgcga
     4741 cagatgacat gtcagccgca ccagctcctg ataagctcct ctccatctgg ttaaaggcat
     4801 tgatgtatgc ctctttgaac cgggcggcac gtttaccagt gaagcccata gcaaggaagg
     4861 cgaagccgcc gcgggtaatt tggtagcagg gaagtttgcg agtaccgccg ttgggctggc
     4921 gtaccaaaat tgatgtctcc gcaaaattgc gggcacaaaa ctctggagaa caatccaaaa
     4981 tgcggatctt tttcagaaca tcgtcatgac gtttagagaa gaagttggca acagccaggg
     5041 atgaagtaac agcctaacca tcaacgatgg caatttcagg ttgagagagg gttgggagag
     5101 tagtcatggt gacagcccct atgttgaatt caaagaactc accacatggg acgccaatca
     5161 cagaggtggt gagacgtaca gggttggcgt aaccggtcaa catagaaccc ggcgcatctt
     5221 gcgatgtccc tgcacgcccc accataattt gggcgtagca atgctcatga cacgaaaaaa
     5281 ccgcatgagc gcggttatgc tctatattga atttcaggac gccaatcccg gcccccgctt
     5341 tataaggtgc agagacagtg taacgtcccg aaattgcaga atcaatatat tgtgcagata
     5401 taacgatttt gttatgtttt gctgtatgaa ctacactatc gaatactaca gtgaagaggt
     5461 ccgactggag gtcgatcagc ttccattgag tatgcgtgcc agataccagc atcttgttga
     5521 acgcatgaag gtatacggca gcaatctcgg agaacctcac accagcgcct ttggtgacgg
     5581 gcttttcgaa ctcagaatta aaggcaacga tgggatcgcg cgtgtttttt actgcactct
     5641 gacagggaaa cgcatcatca tgctgcatag ttttgtaaag aaagcgcaga aaacaccgcc
     5701 agccgaacgc aagaaagctg aaaccagaat gaaggaggtt aagcatgact ggtaaacgca
     5761 ctctccccac catgacacac gacgaaatgg cagccaaatg gatggaagac ccggctttta
     5821 aagcagaata cgacgctatc gctgacgaat tcgcactgct tgatgaaatg ctggcagcac
     5881 gcaaagaagc tggcttaact caggctgaag ttgccgagcg aatgggaaca aaagcgaccg
     5941 cgatcaccag aatggaaagt aatctcgcat caggtatcag cggcccatca tttgccacac
     6001 taaaaaaatt cgcccgcgct actggaaaaa aactccagat ccgcttcgtt taaccacacc
     6061 gcgccgtcat tctggcggcg caccggataa atagtcattt cctcgcacga tgtcttagcc
     6121 accggatatc ccacaggtga gtcgtgtagt tgaaggtttt tacgtcagat tcttttggga
     6181 ttggcttgcg tttatttctg gagcgtttcg ttggaaggta tttgcagttt tcgcagatga
     6241 tgtcggtgaa acttcgtcga tgtcgcctca ttcatacctc ctgtcggtaa atctgacacc
     6301 ctgaccaata gcccaggctg ttgtgtactc gatcagactt gccatgcgct tcacgctcat
     6361 ctgcgcgctg ctttcgcgaa tgttgacgca ttctccttca aggccgggca aaacatcagc
     6421 ttcctgtttt gttgccactg catgaccgct gatcaacaaa accttccatt gttctggttt
     6481 taaccatttg ccgcaccatt gaacctgacg agcgatatcc gccagcatcg cgtgaaattt
     6541 tgcgttctgg tcaagatttc gcttgtagtc agtaatgcgg atggtgactg gcttgtcttt
     6601 atcgagagga gttgagagga tggcatttat tgcggcttgc tgttgttgct tagttcggag
     6661 gaagattgtt tgcttcatcg aaaattcttc tctttaattc cagcggctct gatagctttc
     6721 attactgcaa ttaccgtttt gtccttccca tcctcatgcc ccatcgcata agcaccttct
     6781 tcaccatctt tccaaaagtc gtcattcgat tcgggccagt cgatatccag ttcaatagct
     6841 gctcgcgatg cctgccatat cacccaggca aactctttta attcatcgtc tcctgtgaac
     6901 tggcttttgt cttttgacca ccagttttca aactgtcggt agctatcgtt cacttccctc
     6961 tcccccaaat aaaaaggcct gcgattacca gcaggcctgc tattagctca gtgatgtaga
     7021 tggtcattta atactccgtc acgttttcct gtcgccacgc ctcgtcatat tccgatttcg
     7081 gcatattggc gatgtagcta tatggcgatc ctgattcaag ttgcaggaac tggtgcgatt
     7141 gctcgtcaag gaacaacgga acaccacctt cccaaccttc gccgttacgt tgtttttcaa
     7201 gcatcaaaac agatgc
//
LOCUS       DAJVRL010000153         7192 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVRL010000153 DAJVRL010000000
VERSION     DAJVRL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.153"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..890)
                     /locus_tag="OQN02_003639"
     CDS             complement(<1..890)
                     /locus_tag="OQN02_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1644289.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="OQN02_003640"
     CDS             1131..1931
                     /locus_tag="OQN02_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1644290.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="OQN02_003641"
     CDS             2009..2779
                     /locus_tag="OQN02_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1644291.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="OQN02_003642"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="OQN02_003642"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1644292.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="OQN02_003643"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="OQN02_003643"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1644293.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="OQN02_003644"
     CDS             5046..5768
                     /locus_tag="OQN02_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1644294.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="OQN02_003645"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="OQN02_003645"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1644295.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="OQN02_003646"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="OQN02_003646"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1644296.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
BASE COUNT         1776 a         1767 c         1739 g         1910 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagtgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gc
//
LOCUS       DAJVRL010000154         7171 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVRL010000154 DAJVRL010000000
VERSION     DAJVRL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.154"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(296..1198)
                     /locus_tag="OQN02_003647"
     CDS             complement(296..1198)
                     /locus_tag="OQN02_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCS1644297.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OQN02_003648"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OQN02_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCS1644298.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OQN02_003649"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OQN02_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCS1644299.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5024
                     /gene="lsrC"
                     /locus_tag="OQN02_003650"
     CDS             4008..5024
                     /gene="lsrC"
                     /locus_tag="OQN02_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCS1644300.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAVLGAWFLTQIDSVLV
                     LLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPASSGKK
                     REAA"
     gene            5024..6016
                     /gene="lsrD"
                     /locus_tag="OQN02_003651"
     CDS             5024..6016
                     /gene="lsrD"
                     /locus_tag="OQN02_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCS1644301.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6028..7051
                     /gene="lsrB"
                     /locus_tag="OQN02_003652"
                     /pseudo
     CDS             6028..7051
                     /gene="lsrB"
                     /locus_tag="OQN02_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7078..>7171
                     /locus_tag="OQN02_003653"
     CDS             7078..>7171
                     /locus_tag="OQN02_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCS1644302.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1672 a         1808 c         1874 g         1817 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg gtactcggcg
     4801 catggttcct gacgcagatc gatagcgtac tggtgctgtt gcgcattccg gcatggtgga
     4861 atgattttat cgcgggtctg gttctgctgg cggtgctggt gtttgatgga cgcctgcgtt
     4921 gtgctctgga acgtaatcta cggcggcaaa aatatgcccg ctttatgacg ccaccgccat
     4981 ccgttaaacc cgcttcgtca ggtaaaaaac gggaggccgc ataatgcgta ttcgctacgg
     5041 ttgggaactg gctcttgccg cactgctcgt tattgagatt gtcgcatttg gtgcaattaa
     5101 cccgcgaatg ttagatctca atatgttgct gttcagcacc agtgacttta tctgcattgg
     5161 cattgtcgct ctaccgctga cgatggtgat tgtcagtggc gggatcgata tttcgtttgg
     5221 ttcgaccatc ggcctctgcg ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat
     5281 gccgctggcg atactcctga ccttactgct cggcgcattg tgcgggctga tcaacgccgg
     5341 attaattatc tataccaaag ttaacccgct ggtgattacg cttggcacgc tgtatctgtt
     5401 tgccggaagc gctctgctgc tttccggtat ggccggagcg acggggtacg aaggtattgg
     5461 tggattcccg atggcgttta cagatttcgc taacctggat gtgctgggac tccccgttcc
     5521 gctgattatc ttcctgatat gtctcctcgt tttctggctc tggctgcata aaacccatgc
     5581 cggacgtaat gtgtttttga ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc
     5641 agttaaccgc accttatgtg cgctctatgc catgacgggg ctggcgtctg cggtcgccgc
     5701 tgtgctgctg gtatcgtatt ttggttcagc acgttccgat ctcggtgcgt cgtttctgat
     5761 gcccgccatc accgccgtgg tgcttggcgg ggccaatatt tatggtggtt ccggttccat
     5821 tatcggcacc gccattgcgg ttttattagt gggatatttg cagcaaggtt tgcaaatggc
     5881 aggagtgcca aatcaggtgt ccagcgccct ttccggtgcg ctgcttatcg tcgttgtcgt
     5941 aggtcgttcc gttagcctgc atcgccagca aattaaagag tggctggcgc gtcgggccaa
     6001 taacccattg ccataaagga tatcttcatg acacttcatc gctttaagaa aatcgcctta
     6061 cttagcgctc ttggcattgc cgcaatctct atgaatgtgc aggccgcaga gcgtattgca
     6121 tttattccca aactggttgg cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa
     6181 gcgggtaaag agctgagcgt tgatgtgacc tacgacgggc cgacagaacc cagtgtttct
     6241 ggtcaggtac agttgattaa taacttcgtc aatcaaggtt ataacgccat tattcgtttc
     6301 tgcggtttcg cctgatggct tgtgtccggc actgaaacgc gccatgcaac gtggtgtgag
     6361 agtgctgacc tgggactctg atactaaacc ggagtgccgc tcttactaca ttaatcaggg
     6421 aacgcccgcc cagttgggag gtatgttggt ggatatggcg gcgcgtcagg tgaataaaga
     6481 caaagccaaa gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa accagtgggt
     6541 gaaagaagcg aaagcgaaaa tcgccaaaga gcatccaggc tgggaaattg tcactacgca
     6601 gtttggctat aacgatgcca ctaaatcgtt acaaaccgca gaaggaatat taaaagcgta
     6661 tagcgatctc gacgccatta tcgcccccga tgccaacgcc ctgcccgctg ccgcacaagc
     6721 cgcagaaaac ctgaaaaatg acaaagtagc gattgtcgga ttcagtacgc caaatgtgat
     6781 gcgcccgtat gtagagcgcg gcacggtgaa agaatttgga ctgtgggatg tggttcagca
     6841 aggcaaaatt tcagtgtatg tcgcggatgc attattgaaa aaaggatcaa tgaaaacggg
     6901 cgacaagctg gatatccagg gcgtaggtca ggttgaagtc tcgccaaaca gcgttcaggg
     6961 ctatgactac gaagcggatg gtaatggcat cgtactgtta ccggagcgcg tgatattcaa
     7021 caaagagaat atcggcaaat acgatttctg atgtgcatta cttaaccgga gtaagttatg
     7081 gcagatttag acgatattaa agatggtaaa gattttcgta ccgatcaacc gcaacaaaat
     7141 atccctttta ccctgaaagg ttgcggtgcg c
//
LOCUS       DAJVRL010000155         6619 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVRL010000155 DAJVRL010000000
VERSION     DAJVRL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.155"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            64..2496
                     /locus_tag="OQN02_003654"
     CDS             64..2496
                     /locus_tag="OQN02_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1644303.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2563..4032
                     /locus_tag="OQN02_003655"
     CDS             2563..4032
                     /locus_tag="OQN02_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1644304.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4032..5693
                     /locus_tag="OQN02_003656"
     CDS             4032..5693
                     /locus_tag="OQN02_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCS1644305.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5914..6255
                     /gene="symE"
                     /locus_tag="OQN02_003657"
     CDS             5914..6255
                     /gene="symE"
                     /locus_tag="OQN02_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCS1644306.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6302..>6619)
                     /locus_tag="OQN02_003658"
     CDS             complement(6302..>6619)
                     /locus_tag="OQN02_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644307.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1950 a         1581 c         1526 g         1562 t
ORIGIN      
        1 tcatagccag gctattgatt ttatgtacaa tcagaatcgt cctgataaaa gcgagaaccc
       61 cccatggcgg aactgaacct aagtaacctg acggaagcag acatcattac caaatgcgtt
      121 atgccagcca ttctcaatgc aggctgggac aacacaacgc aaatcagaca ggaggtcaaa
      181 ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg cacgcagaac ggtaaaatct
      241 gctgacatcg tgctgtacca caagccaggc attcccttag ccgtgattga agcaaaagcc
      301 aacaaacatg aaattggcaa agggatgcaa cagggcattg aatatgcgcg cctgctggac
      361 gttccttttg tttttgccac caacggtgat ggctttatct tccgcgatgc caccgcagcc
      421 gaaggtgaat gccttgaaaa gcaaataacg ctggatgact tcccctcccc cgctgaactc
      481 tggcaaaaat tctgcctctg gaaaggatac acacaagctc agcttccggt gattactcag
      541 gactattacg acgatggcag cggcaaatcg cctcgttatt atcaacttca ggcaatcaac
      601 aaaaccattg aagccgtctc caacgggcaa aaccgcgttc tgctggtcat ggcgaccgga
      661 acagggaaaa cctataccgc attccagatc atctggcgcc tgtggaaatc aaaaaataaa
      721 aaacgcattt tgttccttgc cgatcgcaac attctggtcg accaaaccaa aaataatgat
      781 ttccagccat ttggtacggc aatgaccaaa gtcagcggac gcaccattga tcccgcttat
      841 gaaattcacc tcgcgctcta tcaggctata actggcccgg aggaagacca aaaagcgttt
      901 aaacaagtcg caccagattt cttcgatctg atcgtgatcg acgaatgcca tcgcggcagc
      961 gcatccgaag acagcgcctg gcgagagatc cttgattatt tcagttccgc cacccaaatt
     1021 ggcttaaccg ccacgccaaa agagacgcat gaagtctcca gcacggatta cttcggcgat
     1081 ccggtttacg tctactcact aaaagaaggg atcgaagacg gcttcctcgc tccttataaa
     1141 gttgtccgtg ttgatattga tgtcgacctg caaggctggc gcccaaccaa agggcaaact
     1201 gacttaaacg gcgaagtgat tgacgatcgt atctataacc agaaagattt cgatcgcacg
     1261 atggtgatcg acgaacgcac agaactggtt gccagaacca ttaccgacta cctcaagcgc
     1321 accaatccga tggataaaac catcgtcttc tgtaacgaca tcgatcatgc agaacgtatg
     1381 cgccgcgccc tggttaatct caacccgcag caggtgaaaa agaacgacaa atacgtcatg
     1441 aaaatcaccg gcgatgatga aattggcaaa gctcagttgg ataacttcat caacccgaaa
     1501 aaaccgtacc cggttatcgc gaccacttca gagctgatga ccactggtgt ggatgctaaa
     1561 acctgcaaac tggtagtact agaccagaac atccagtcga tgaccaaatt caagcaaatt
     1621 atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac tctggtttac catcctcgac
     1681 tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg atggcattcc cgaaaaagtc
     1741 atggatacca caccagagga tatcgccgat ccagaatctg attttgaaga gaaactcgaa
     1801 gaaatcagcg aacatgacga cgaacaggta acaggcgttg atgaaccgcc tgcgccacca
     1861 taccaggtta ccgataccga tgatgtcggc ccatttccgg aagaagacga gaagaaaatc
     1921 cgcaagtttc acgtcaacgg tgtagcggtg ggcgttattg cacagcgcgt tcagtattac
     1981 gacgccgacg gtaaactggt taccgaatcc tttaaagatt acacccgcaa aacactgctc
     2041 aaagaatatg cctcgctgga tgactttacc cgcaagtggc aggacgccga tcgcaaagaa
     2101 gcgatcattc acgagctgga gcaacagggg atcatctggg aagtactggc agaagaagtc
     2161 ggtaaagatc tcgacccgtt cgacatgctt tgccacgtag tgtatggtca gccgccgtta
     2221 acccgcaaag agcgcgccga gaacgtgcac aagcggaact acttcacaaa atactctgaa
     2281 gcagcgcaag ccgtgctcga taatctgctg gataaatacg ccgatgcggg cgtacaggag
     2341 atcgaaagta ttcaggtgct gaaacttaaa ccattcgaca gcatgggcac cttaccggag
     2401 attattaaaa ccggatttgg cgaccgtaac gggtataatc aggcgctcag cgagctggaa
     2461 aacgaaatct accaattacc gccccgctct gcttaacaac tctcttttgc gccttctgca
     2521 accatcatgt tgcagaaggc cttgtttttc atggaataca acatgtctat cagctcagta
     2581 atcaaatcat tacaagatat tatgcgcaaa gatgccggtg tggacggcga tgcgcagcgt
     2641 ctcggtcagc tctcctggct gctgtttttg aaaatcttcg atgcccagga agaggcgctg
     2701 gaactggagc aggataacta tcaatatccg atcccacagc gttatttgtg gcgcagttgg
     2761 gccgccaacg cgcagggcat taccggtgat tccctgctgg aattcgttaa tgatgatctg
     2821 tttccggcgt tgaaaaacct cactgcgcct atcgataaaa acccacgcgg ctacgtggtg
     2881 aagcaggcgt tcagcgatgc ctataactat atgaaaaacg gtacgctact gcgccaggtg
     2941 atcaacaagc tgaacgaaat tgactttacc agcgccagcg aacgccatct gtttggtgat
     3001 atttacgaac agatccttaa agatctgcaa tctgcgggca atgcgggcga attctatact
     3061 ccacgcgccg tcactcgctt tatggtggat cgcgttgatc cgaaactcgg cgaatccatt
     3121 atggacccgg cctgcggtac gggcggtttt cttgcctgcg cgttcgatca tgtaaagaac
     3181 aaatacgtga agagcgtcgc cgatcatcag acactgcaac aacagatcca cggtgttgag
     3241 aaaaaacagc ttccgcacct gctggcgacc accaatatgc tgctgcacgg cattgaagta
     3301 ccggtacaaa ttcgtcacga caacaccctg aataaaccgc tttcctcctg ggatgagcaa
     3361 ctggatgtca ttgttaccaa cccgccgttt ggtggcacgg aagaagacgg tattgagaag
     3421 aactttccgg cagagatgca aacccgcgaa acggcggatc tgtttctgca actgattgtg
     3481 gaagtactgg cgaaaaacgg tcgtgcggcg gtggtattgc cggatggcac actatttggc
     3541 gaaggtgtta aaaccaaaat caaaaagcta cttaccgaag agtgcaacct gcataccatc
     3601 gtgcgtttac cgaatggtgt gtttaatccc tataccggca ttaaaaccaa cctgttgttc
     3661 tttaccaaag gtcagccaac caaagagatt tggttctatg aacatccgta tccggcgggc
     3721 gtgaaaaact acagcaaaac caagccgatg aagtttgaag agtttcaggc ggagatcgac
     3781 tggtggggta acgaggccga tggttttgcc agccgcgtcg agaatgagca agcgtggaaa
     3841 gtcagcattg atgacgtgat tgcgcgcaac ttcaatctgg atattaaaaa cccacatcag
     3901 gcggaaaccg tcagccatga tccggacgaa ctgttagcgc agtatgcaaa acagcaggcg
     3961 gagatccaga cgctgcgtaa tcaactgcgc gatattcttg gcgctgcgct gtctggcaag
     4021 gaggttaact aatgagtgtg gaaaagctga tcgttgatca tatggaaacc tggacctcgg
     4081 cgttgcaaac ccgttccacc gccgggcgcg gcagctccgg taagattgat ttgtatggta
     4141 ttaagaaatt gcgtgagctg attctggaac tggcggtgcg cggtaaactg gtgccgcagg
     4201 atccgaatga tgaaccggcg tcggacctgc tgaagcgtat tgcggcggaa aaagcagagc
     4261 tggtgaagca aggcaaaatt aaaaagcaaa aaccactgcc agaaattagt gaggaagaga
     4321 agccgtttga attgccggag ggatgggagt gggtgcacct ccctgatatt tattgctcaa
     4381 tttctgagtc atctagaaaa ataaaatcct ccgaaatact acctgaggga aaatatcctg
     4441 ttatcgagca aagccaagaa tttatttctg gttattgcaa caatgaatgt ctgttaataa
     4501 agttaaataa tccagttata gtttttggtg atcacacaag gaatattaaa tttattgatt
     4561 ttgattttgt tgtcggcgct gacggtgtga agattttatc acctatatta atttgtgagc
     4621 gtttcttttt ttggcagtta cgatcattta aattagatgt aagaggatac gcgagacatt
     4681 ttaaagtatt aaatagctgc ttatttgcat taccccccat tgcagaacaa gagcgaatag
     4741 ttgaaaaagt tagctctttg atgtccctct gcgaccaact agaacagcaa tccctgacca
     4801 gtctggacgc acatcagcaa ctggttgaaa ccctgttggg aacgcttaca gacagccaaa
     4861 acaccgcgga actggctgaa aactgggcgc gtattagcga gcatttcgac accctattta
     4921 ccaccgaagc cagcgtggat gcgttaaaac agaccattct gcaactggcc gtaatgggca
     4981 aacttgtgcc gcaggatccg aatgacgaac ccgcctctga actgctcaaa cgtattgcgc
     5041 aggaaaaagc tcaattggtg aaagaaggga aaataaaaaa acaaaaacca ctgccgccaa
     5101 ttagcgatga ggaaaaaccg tttgaacttc cggaagggtg ggaatggtgt cgtattggta
     5161 atatagttaa tattaaatct gaattagtat caccaaaaga ttatttaaat ttatatcaag
     5221 tagcacctga tattattgaa aagggcactg ggagagtgat atcaaagaga acagtgaaag
     5281 aatcaggagt caaagggcca aatagtcgct tttataaagg acaaattgtt tattctaaaa
     5341 tccgcccgtc attatccaaa gtttttttag cagagtataa tggtctttgc agtgctgata
     5401 tgtatccatt agactgttac attaatccaa attacttatt gaaatatatc ctttccattc
     5461 catttcttat gcaagtcaaa aaagcagaga acagaataaa aatgccaaaa cttaattctg
     5521 acagttttta caatattatt gttgcgattc ccccctataa tgaacaacaa gcaatatttg
     5581 ataagataaa ttctattgaa gcagtatgca atggtcttat ttcttatatt gggatttatc
     5641 ataaaaccca actgcatctt gcagacgcac tcactgacgc agcgataaac taattccctc
     5701 gccctcacaa aaccaataaa gcggctaaat agccgcttta ttcacatcac caaaatttat
     5761 atttcacgct aattttcatc ttattttcat taatttttag ctggactttc cctgtattta
     5821 ctgatgatgt atccaggtat ttagcacggt gcggatgtgc gccaacacac tcgcaccgct
     5881 aatcacaatc actatttata gagaatagta atcatgactg acacgcattc tattgcacaa
     5941 ccgttagaag cagaagtctc cccggcaaat aatcgtcaat taaccgtgag ttatgcgagt
     6001 cgttacccgg attacagccg tattcccgcc atcaccctga aaggtcagtg gctggaagcc
     6061 gccggttttg ccaccggcac ggcggtagat gtcaaagtga tggaaggctg tattgtcctc
     6121 accgcccaac ccgccgccgc cgaagagagc gaactgatgc agtcactacg caaggtgtgt
     6181 aagctgtcgg cgcgtaaaca aaggcaggta caggaattta ttggggtaat tacaggtaaa
     6241 cagaaagtcg cctgatacat tgacccggta gcgccagtgc taccgggtat tattcgccgc
     6301 gttataccaa cgcgctctcc atctgttttt caaatgcctg acgaattaat cccataatgt
     6361 aaggaagttg tgcgaggtcg ctgaaaccaa tttctacatc gccattaccc cagcgcccaa
     6421 catttgtcac atctttggca atacctttcg gatcgaccag ttcatgaaac tgcatattaa
     6481 gcgacaatcg cagtcggctt ttttgcggca caacatcaac aaaattcgtt tccgctttaa
     6541 acgcaatata tagcttcaat acttcctgcg tgatccctgc gtccaggcgc ataacttcat
     6601 cgcgaagatg atcgaataa
//
LOCUS       DAJVRL010000156         6611 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVRL010000156 DAJVRL010000000
VERSION     DAJVRL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.156"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(93..2096)
                     /locus_tag="OQN02_003659"
     CDS             complement(93..2096)
                     /locus_tag="OQN02_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644308.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2093..3229)
                     /locus_tag="OQN02_003660"
     CDS             complement(2093..3229)
                     /locus_tag="OQN02_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1644309.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3222..5501)
                     /locus_tag="OQN02_003661"
     CDS             complement(3222..5501)
                     /locus_tag="OQN02_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644310.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5512..6600)
                     /locus_tag="OQN02_003662"
     CDS             complement(5512..6600)
                     /locus_tag="OQN02_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCS1644311.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1510 a         1822 c         1776 g         1503 t
ORIGIN      
        1 gtgactctca ccaggggcta tataggatat gcctaatacc gtggcgtgag cagtccggaa
       61 ctggagtaga actcttagta aaaagcacta tttcatcctt gttgctgaag catggggaat
      121 aattgttcgc aaagcaaaac accgttattc attgcttcta cccgtgcctc gctttctgta
      181 ttacgaaatt gtgccaacac atgtgccagc cgataaaaac ccaccgcggt gaggtcattc
      241 gccagcaact ctgcctgatt aatagcgctc tgttcctgat agcgccagcc gttatggagc
      301 agttgaataa gtaacgcctg gcagcgcatc agcaactgat gagcagtaga cggcacaggc
      361 agaacgctgg cagaagatag tgataccacc acaggcgcag tttctgcgtc cagcgcccag
      421 gcacgagtct ttgtcatcat tacctgtggt tccagtgtca attgcccatc aacaaaactg
      481 acaaagccag aaaccagaca cacggggtcg tctgtttgtt gcaaaagcgc cgccatgcgt
      541 tcaatggcat aaggcgcgct ggctgaggct ggcagtgata acgtcagcag attatcttca
      601 ccttcgccgc tgattacctg cgcatccagc gtctggcggc tgctatccca accgagcgaa
      661 atacactcag cgaccggcag aataaataag ttatcgacct gattaagagg ccgtatgcag
      721 gcggggggac gctggcgtaa atattcccgc aaagccacaa tgcccggctg gcgtaacggc
      781 gcgctcaaca tttgccaggc atcaggcgac agcggtacaa cgctgcttaa gcggttgcgg
      841 gtagctaaca gcagctcgcc atcggcactg cgttttgctg cttgtgaaac aatttgccca
      901 cccgccagtg cgccagcctg aaaactaaac agccgacgcg tagctgccgg tgagtcttcc
      961 tgttcacttc gcggccaact gcgcgaaagg tgcaaaatac tgccggtgtc gggatcggta
     1021 aaccagatgc gtaaaccata atgctcaata tcctgccagc aacgcatacc taaagacacc
     1081 agccgcagat gatcaagctt tgcttctccg gcaatgccag agccaacgac cgtgcgccac
     1141 ggcacaggag gaacttcacc aacactgtcg ctccgggcca tctcttgtgc gcaatttaat
     1201 cgactgttta atgccgcaag ctgacgtaag cattctccgg catgatagtg gctggcgcgg
     1261 gcgtggaagg catcaacgct ggcgcgcagt tgccgtagcg attcactcac ccatcgccag
     1321 ctgcagcgtt ccgctgcttg ctgcgcgcgg ctgaaagcgg cctcgtagtg aataagcggc
     1381 tggctgatgc cgctcagcca taatgcctgg cttagttgtt gaacatattg acgacacgtt
     1441 ttgccttctt cgctggcaaa cggatcgtca gatgatgtga cgtgttcgct gcgcatctgc
     1501 cagattaaat gagtcaattc cgcttgctgc gcttttgcct ggacaaaggc ctgtactgcc
     1561 agtacgacat gttcgcaaag tgtgccttca atacaatcac aacgggcgaa acgaatactg
     1621 ctgcgggaat aaaaacgcac atcgctcatc ggtaagcggg cagagggaat ttcacccggc
     1681 gcacagaaca actcaatggt gatgctttta gcgaccagcg cctgtgcgcg tttgcgggtg
     1741 gcatcgggaa gggtagccag ttcttccagc cagattgccg gatcccattc ctcttctttt
     1801 cccgtaggct gggcggtagc acaaagtcgt tgataactta acaccagcat cacgcgatga
     1861 cggcacatgc cgctggcccc ataggtgcac tgagcctctt tcagtgcctg gccgttcgcc
     1921 agctgggtac ggacaccgtc actgaaggtg gcgattaaag cgctgttctc atggctgatt
     1981 tccgggacgt tgccattttc cagttcctta aggctgcgct taacaaaacc ggcattgctt
     2041 aacgccgtca gtgcctgcgg tgtcagttct aataattccg gacgtagtga attcatgact
     2101 gaagattctc cgcaagccat gatgccagct cgcccggtgt catggcggct atttgtgcgc
     2161 cgacattaat cagcgcctgg gccgtatcgc ggtcatagca aggtgttgct gtgctatcga
     2221 gcgctgccag tcccagcact ttgatgccgc tctggacaca ctttttcact tggtgcgtca
     2281 gcaatgatga tgaaccccct tcataaaaat cgctcacgag gataatgacg ctttttgcag
     2341 gttgttcaat aagttgccga ccatactcca tggcactggc gatattggtt ccgccgccta
     2401 attgcacttt cattaataac tctaccggat cggcaacgtc tgccgtgaga tcaacgacgc
     2461 ttgtgtcaaa cgccaccaga tgggtacgaa tgccgggtaa ctgccataaa caggccgcca
     2521 tcaccgcaga gtggatcacc gaatcgacca tcgatccgct ttgatcaacc agtaagacca
     2581 gttgccattg ctcgctttgg cgtttaatgc ggctgttaaa gcgggggaat tcgatataca
     2641 acttgccgtg ttgcgggtgc cagtgttgca ggttggcgcg cagagtactt ttgaaatcaa
     2701 agtttcgcgc cagtggaata aatgagcggc gacgacgatc gcggacacca gaaaaagcct
     2761 gacgaacttc ctttgccagt cgcgccataa tttcttccac aacctggcgc acaatcctgc
     2821 gggcggcagc cagtacttcg gggttcatca gatgtttggt gtgcaaaacg gcgcgtagca
     2881 ggctttcgga aggctgcata cgttccagca cgtcgagatt tgtcaccaca tcttcaatgc
     2941 cgtagcgcag tacggcatcg ctttccagcc gctcaatcac ctgttgcgga aacagcgtgt
     3001 gaatactgtt gatccactca ggagtggtga gatttgagcc acctaatcca ctagagcgtt
     3061 caccacgctg gagccgttca ggatcgcgcc catacagcca ctccagcgcg tgatctatct
     3121 gccgggcgtt gtcatccagc ccacaaagcg tcgtttctgc cgcttcgcca agaattaatc
     3181 gccagcgttg tagctcacgg gtggtcagaa gatcgttcag ttcagacatg gaaaactccc
     3241 cagtgttgca gcgatgccag tgcctgctgt tcgagttgtt gatgatgtgc aatggcttgc
     3301 ggtggacaat gcaacggcat ttgcagtgcc gaaacgggaa gttgcgccag ttgataatgc
     3361 tcaagcacct gatgcgccag cgtcccgcgt tctcgtggcg gtagccaggc cattgccgcg
     3421 cgtaaatcgg gcagggcatt ggtaaaatcg gcatcactta gttgatttaa atgactgctg
     3481 aaaccggcga taaatgccgg ctgacaggcc agttgatgac gggctaatgc cagcaaaccg
     3541 tgcagcgcct cgccggattg tgccggggat aactgcgcca gcatcgtcag cgccgcttcg
     3601 gcactagcgt tgggatgttc cagacgcatc agcgcaccaa gagtcgcgcc gcgatccagt
     3661 gccaggacat gaatcgcctg actgtgtcgc tccagcaagg ctaccgccgc agaaagcgaa
     3721 acgccgggga gttgcacacc gctatgtaga tcgcgcagga tatgacacaa cgcttgccag
     3781 ctatgcaagt gagcgtgaaa ctccttttca tccggtctgc cgttggattc acacagccac
     3841 agcgtgcgat cgatagccgc gcataacgtc gtttgtaata tctgcgcgcc ttgcataccg
     3901 ctaatttcat ccagccgcca taaggcatat agcacttcca gcgcctggcc catttcggca
     3961 aattgatttt cctgggcgat taataatgtg agttgctcca gtaattgttg actgaaggac
     4021 gccagacccg ctgacgccgc ttggctaaga catgtggtaa tgctgccgat tccacccgcg
     4081 tccagcatat cggcttctaa tttatggcgt gcggcttcct ggagtgtggc accaaagcag
     4141 gcggcctcaa tcaatgccgc atgctggcta agcgggcggg ttaatttcca gcgttcttca
     4201 ccgttgcctg caagtgtcag tgtgcttccc tgctggcgta caatccctgg gatctccagt
     4261 attgccagcc gatgtaacac ctgactttgc gctagtccat tggggttaaa gcgatttaag
     4321 gtaagctccg ccggaagaga gattgcggta cgttccagtt cgcaagtgac atcttttggc
     4381 agaggcggtt gtggcgtaga aggggcaagt ttaccgaatc cgtcacccgc taatgtgtct
     4441 atcaacgtta gcagaatcgg atcggtatcg ggatgaataa cgccgcgata gctccacggc
     4501 aacggcgcat tcagggcttc tttaatcagc gatcctgcta tggcatccag ccagtcagtg
     4561 cgtaacggta gtgtatgacc gcgcaactgt gccagtgcca tcgcatgcag atgagcggca
     4621 gccatatccg cggtcgaagc aggcaacttg tgctggcgca aacgggtgag aaccgttttt
     4681 agcagttgtt ctcccgcctg ctgtaagccc cactgccagc accagttttg ccagaccggg
     4741 gcaggcattc ctgaaaggta tcctgccagc acatcaaggc gcttttcact gtagggggtg
     4801 agataacaac ctgtaacggc atctgccagc gacggcaatt ctggcgtgtt aattacctgc
     4861 ggacattcgc gccacatttt tgccagtgcc ggagcgtgcc agccaccgca gacaactaac
     4921 acgtcgccat tattttgctg catcgcccat ccaatccagc gggccataaa ggcttcgcgc
     4981 tgacgattta gagcatcgcc gggggagtcg ccccgcagtt gggcaaaata gtgcgccagt
     5041 gcagagggta atgcagtttg ctggctttca tcttcgaaca agtgatccca cagggtgtcg
     5101 ctgttatcca tgcgggtggc acgcagcagc aaggtctggc tttcctcttg cgtatcaggc
     5161 gaatcgtcct cttcttcact ctgcgcccag caaggcaaat cgatgaagta agtttgtgcc
     5221 tgaatacgac gatcggcttg tagcgcctgc cactccggcg aaaattcagc aaatggcgtc
     5281 caggcaccac gcccgggggc tgcaccgtcc tgatactggc aataactgta aatagctacc
     5341 ggaagctggt gggctaaaaa cagttcgtct acccggtcat taaaatcagc agggccttca
     5401 atcaacacgt atcgtggccg ctggctttcg attaacgatt tcaccagccg ggcgcaggcc
     5461 ggactatgat gccggatgcc gacaattaac ggctcgctca tgcatctttc ctcacggcag
     5521 gcggtggcgg gcttgataat aagcctgcca gtgagcttct ttatgtgtgg cgacacgctg
     5581 ttcgaagtaa cggcgcagac gagcgcgatc ttcttcgtta tctttgacga tggttccggc
     5641 aatgcagtcc accaggtcgg ctggctcacc tgcgcgattc gctaaaaacc aggcgcggac
     5701 gcctacggcg tgggccacgt tcacggcttc agcggtggac ataatcgctg ttagtgtatc
     5761 cattgacgtt ttcttttcgc cgttggcacg cagatcgcgg aaggtgcgga ccagtaactc
     5821 cagtaccgcg tcaggcactt tatgcggaat accgctatgg gccagcaaac gcgccgaagc
     5881 actggcgaca agctccagct cctgggcgaa atccataatc ggaaagacag tttcaaaatc
     5941 gaagcgacgc tttagcgcag cactcatttc attgacgccg cgatcgcggg tattggcagt
     6001 ggcaataata ttaaagcctt ctcgcgcata gagctgactg gcttcaccag tgagttccgg
     6061 caccgtcatc accctgtcag aaagcatacc taacagacag tcttgcactt ccagcggtgt
     6121 acgcgttatc tcttcaaaac ggacgatttt gccgtcgcgc attccctggt aaagcggggc
     6181 agggaccagc gcttccgttg atggaccatg attgataagc agggcgtagt tccagccgta
     6241 cttgatttga tcttcggtag tagatgcgcc gccctggatg gttaacccgg catcgccgct
     6301 aatagaggtt gccagtaatt cagacaggag agattttgcg gtgccaggtt cccccaccaa
     6361 catcaaaccg cgtccggtgg caagagtgac taacatacgt tcgacagcgg agggactaat
     6421 caccacttta cggctaatat tttgcgcgct atcgccaagg ataaaagcac gggccgcagg
     6481 caaacttaac tgccaaccgg gcgggcaagg tgcgttatca ttttgtttta attttgccag
     6541 ttcatcggcg tataacacag cagcaggcgg acgctgcaga tgattattct gcggtgacat
     6601 atattcatcc c
//
LOCUS       DAJVRL010000157         6403 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVRL010000157 DAJVRL010000000
VERSION     DAJVRL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.158"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OQN02_003663"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OQN02_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCS1644312.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OQN02_003664"
     CDS             complement(1433..1759)
                     /locus_tag="OQN02_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCS1644313.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OQN02_003665"
     CDS             1894..2235
                     /locus_tag="OQN02_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCS1644314.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OQN02_003666"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OQN02_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OQN02_003667"
     CDS             complement(2833..3543)
                     /locus_tag="OQN02_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCS1644315.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OQN02_003668"
     CDS             3651..3956
                     /locus_tag="OQN02_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCS1644316.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="OQN02_003669"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="OQN02_003669"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="OQN02_003670"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="OQN02_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1706 a         1628 c         1501 g         1568 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatattatt atattaacaa
     6001 caggtccaga ccattggtcc agaccatagt tagttaatca cagtgatagc ccaccacctg
     6061 aagtcctgct gatattcagt ccagacttac ctctgctcat ggtcttacct ccctcggtct
     6121 cctgaccttg ggtatcgact acagcttgct gagcgtcatc tggaggagct acaggaggcg
     6181 ggaccgaagc cccaccttga gtcccaccac cacctgtcag gcccttggcg atggacctga
     6241 agcctttact aatgctctta cccattggtc ttacgcttcg gtcttggtga acttcaggcg
     6301 tttgactaca ggcaggacgg cagtagctga gccagtgccc atcttgacgg tcgtagcagc
     6361 ttcagcgaca acgaacactt taccgaggat ttccaagtcg tgg
//
LOCUS       DAJVRL010000158         6374 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVRL010000158 DAJVRL010000000
VERSION     DAJVRL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.159"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            58..1384
                     /gene="pgaC"
                     /locus_tag="OQN02_003671"
                     /pseudo
     CDS             58..1384
                     /gene="pgaC"
                     /locus_tag="OQN02_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1386..1799
                     /gene="pgaD"
                     /locus_tag="OQN02_003672"
                     /pseudo
     CDS             1386..1799
                     /gene="pgaD"
                     /locus_tag="OQN02_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="OQN02_003673"
     CDS             complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="OQN02_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1644317.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="OQN02_003674"
     CDS             complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="OQN02_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1644318.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="OQN02_003675"
     CDS             complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="OQN02_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1644319.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5701..>6374)
                     /locus_tag="OQN02_003676"
     CDS             complement(5701..>6374)
                     /locus_tag="OQN02_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCS1644320.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1534 a         1680 c         1533 g         1627 t
ORIGIN      
        1 accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat ccgaaaaatg
       61 attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc cctatgcgta
      121 gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg
      181 tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg
      241 tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt
      301 ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt
      361 tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc
      421 ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaacag
      481 gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc
      541 attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg
      601 tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg
      661 gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag
      721 cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc
      781 ctggcagaag tgggttactg gaatgacgat atgatcaccg aagatattga tattagctgg
      841 aagctgcagt tgaatcagtg gacgattttt ttacgagcca cgggcgctgt gctggatatt
      901 aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc
      961 agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc
     1021 gctgtttttt gaatactgcc tgacgacaat atgggccttc acctgcctgg tcggtttcat
     1081 tatttacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc
     1141 gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct
     1201 gatgatcgag aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt
     1261 cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat
     1321 gttgatgcct aaaaagcaac gcgcccgttg ggtaagtcct gatcgcggga ttctgagagg
     1381 ttaatatgaa caatttaatt attacgaccc gacaatcacc agtacgttta ctggttgatt
     1441 atgttgccac aaccatcttg tagacattat ttgctttgtt catattctta ttcgccatgg
     1501 atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt
     1561 tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt
     1621 ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct
     1681 tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact
     1741 tcaccagcca ggggcaaata aaaatggttg tttcagaaaa agcgctagtc cgggcataaa
     1801 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
     1861 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tcttttccga accggacgat
     1921 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
     1981 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
     2041 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
     2101 aactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcggttt ccaccttccc
     2161 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcaagtc
     2221 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tcaccaggta agaagccaag
     2281 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
     2341 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
     2401 ggcgaatatc agctgcttac tctcaatagc ctgcagatag tgcaattgcg cttcatttcg
     2461 cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
     2521 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     2581 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     2641 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     2701 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     2761 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagaaaggaa gtgagaaaaa
     2821 tacagagtga cgaagttgct accggaggag aaatctccgc gagtggtgga gcgttgctta
     2881 acaaaacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     2941 ctgccgggaa cttccgctgc tacttaataa gtacaacaga actcgcattc attgcaacaa
     3001 tatattcact tatatttaac gataaacacc catttcggtg acattaattt ctactggaaa
     3061 gatgacagag tgatgacagt gatggaaaaa gctgtgtgct ttccgcagga ttcgcagggc
     3121 tggtaaaatg tgcctcgtgt ttgccggatt cggcgcgaac gccttacccg acctacaagt
     3181 gattaataat ttgtgcacaa aactcatgcc cgataaacct gcgcatcggg catatttgcg
     3241 tcgttcaaac ctgcaatagc gcccttccca gataatcgtt cgcgtccttc actcccggca
     3301 gcgaaaaaaa ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga
     3361 gccttttttg tactgtcagg aagccttttt ccagatcgtg ttggtagcag acaaacagca
     3421 accccatatc cagttgtccg gagttggtga cccccaacga ataactgtag ccgcgacgca
     3481 gcatcaggct ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca
     3541 gcgcgatcac cttcccttcc gggtcgctgg cgtaatcagg cacatcatgc tcatgctgca
     3601 ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat cgtctgctgt tctttcagcg
     3661 gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac
     3721 cgattgtcca cgcaggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact
     3781 tatcattctg gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg
     3841 tctctttgcc tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca
     3901 cactgagcaa atccggcgtg tgtttgatga tatcgcgcag cgcatggttc accgtgtcct
     3961 gggtgttggc gcaaatctgt agcaacacat caccatgaca taacgccgca tccagcgagt
     4021 cgttggggaa acgcgtcatc ttctgcagct tttttggcat ctgtggcgca aggccaaagc
     4081 gctcatcaaa caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt
     4141 agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgctg
     4201 ctccgccctg actcagaaaa gcaaaacgct gagtcaacaa acgaaacaac cgctcaagat
     4261 cggctttatc gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg
     4321 tcaggatccc tgcctgatgc tcaccataaa acggctgttt ctcattgcgt gcatccggtg
     4381 aaagcgtgcc cggtgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca
     4441 gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg ttttcatctt
     4501 tatactgcat aacgcttaat ccagtcccag cacaccgcga agttgcgcca gatcttccgc
     4561 cagcgcagta atcggccctt tcagcgcatt ccggtcggca tcggtcaatt tgtcgtaggt
     4621 ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc
     4681 atcgactttt gccaacagtt ccgggttggc tttttgcagt tgtggacgca gcagatcgac
     4741 aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt ggctgtagcg
     4801 atcttcttca ccgctgattt tgctggctgc cacttcctca atcagtccgg ctgcgccgcc
     4861 gaccactttt gaaggtggga aagccagttc actgatgcgt ttttgcaaat cgaccacatc
     4921 ggtataaagc tgctcagcgt actgatccat ccctttggtg gtgttgtcgc caaacaatgc
     4981 tttttccaga cggtggaaac cagtgaattt tgggtcggcg gctttttgct cgtaatcatc
     5041 ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg gttcaatacg
     5101 ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat cgcctgcttt
     5161 aatcgcgtcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg ccatgacata
     5221 cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt gcgccgcatc
     5281 cgccgttgcc tcacctttga cgatcaactt ccctttcggg ttagtcagca gaccgcaggt
     5341 catatcgtat tcgccaggct gtaaattcgc cgtcattttc tggctaaaac caggggcgat
     5401 attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca gcgccttctg
     5461 gctatggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg tcatcggttc
     5521 gcactgctta tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt tagccataaa
     5581 agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt
     5641 catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg
     5701 ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc
     5761 cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggtgctt cctgatagcc
     5821 aaaaatccct tccatcagcg tgccaaacag cgagtgagtt gagaggaccg cgctcatatc
     5881 gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc gaatggcacc
     5941 agccgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa aaaatgcacc
     6001 aagattgagg cgaataccgc cccagtagag caggaagccg agcaccacgg cagtagcaag
     6061 accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaatg ccgccagcag
     6121 gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca tcaccagcgc
     6181 ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgttcca gttgcacttt
     6241 gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca ccacggcgat
     6301 caccgccacg ataccttcaa acagttcctg ctctttttgc ggaaattcgc cggtggtttc
     6361 gttaatgaag atcc
//
LOCUS       DAJVRL010000159         6335 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVRL010000159 DAJVRL010000000
VERSION     DAJVRL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.160"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            254..1642
                     /gene="narU"
                     /locus_tag="OQN02_003677"
     CDS             254..1642
                     /gene="narU"
                     /locus_tag="OQN02_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCS1644321.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINDGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1724..5464
                     /gene="narZ"
                     /locus_tag="OQN02_003678"
                     /pseudo
     CDS             1724..5464
                     /gene="narZ"
                     /locus_tag="OQN02_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5461..>6335
                     /gene="narH"
                     /locus_tag="OQN02_003679"
     CDS             5461..>6335
                     /gene="narH"
                     /locus_tag="OQN02_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1644322.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1493 a         1685 c         1662 g         1495 t
ORIGIN      
        1 attcacgatc tgaagggttg aaacatgaca tacgttcaaa gattaacccc cgtttgaagg
       61 ttagcttgtt agttacattt agtaacacat atctggctac ttaatcccca ttctgccccc
      121 gcggttacgc tttcttatct attctttcaa tcagcacttc acccaattca tccttaattc
      181 taataactgg ctattgcgcc ttacctcctg ttttgtcttt tttcattacc aatgtgtgca
      241 tatgaggaac aatatggcac tgcaaaatga gaaaaatagt cgttatcttt tacgcgactg
      301 gaaaccagaa aatccggcct tctgggaaaa taaaggaaaa catattgctc gaagaaacct
      361 ctggatatca gtcagttgtc tacttcttgc cttctgtgtc tggatgctat ttagcgcagt
      421 taccgttaat ctcaataaaa tcggttttaa ttttactacc gatcaactct ttttattaac
      481 cgcattaccc tccgtttctg gcgcattatt gcgtgttccc tactccttta tggtgcctat
      541 attcggtgga cgccgatggc cggtttttag tactgcaatc ctgattattc cttgcgtctg
      601 gctcggaatt gccgtgcaaa atccgaatac tccttttggg atatttatcg ttatcgcttt
      661 gctatgcggt tttgcaggtg caaactttgc ttcgagcatg ggcaatatca gtttcttctt
      721 tccaaaagcc aaacaaggga gcgctcttgg gattaatgac ggattaggaa acttaggtgt
      781 aagtgtaatg cagctggttg caccgctggt catttttgta cctgtatttg cctttctcgg
      841 cgtcaatggc gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat
      901 ttgggtaccg ctactggcga ttgccacgat cgccgcctgg tcagggatga atgatatcgc
      961 cagttcacgc gcctcaattg ccgaccagct ccctgtctta caacgcctgc atctctggct
     1021 gctgagcctg ctttaccttg ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc
     1081 catgctggca aaaacccagt tcccggatgt gaatattctg cgcctggcgt tctttggccc
     1141 atttattggt gccatcgcgc ggtcggttgg tggtgctatt tccgataaat tcggcggcgt
     1201 gcgggtgacg ttgatcaact ttatttttat ggcgattttc agtgccctgc tgttccttac
     1261 cttaccgggc acaggctccg gtaatttcat cgccttttac gccgtattta tggggctgtt
     1321 tctgaccgcg ggtctgggaa gtggttctac tttccagatg atcgccgtca tctttcgcca
     1381 gataaccatt tatcgggtga agatgaaagg cggtagtgat gagcaagctc agagagaagc
     1441 cgtcaccgaa acggcggcaa cgctgggctt tatctcagcc attggcgcag tgggcggctt
     1501 ttttattccg caggcatttg gcatgtcgct caatatgacc ggctctccgg tcggcgcgat
     1561 gaaagtgttt ttaatcttct acatcgtttg tgtgctgctg acctggctgg tttatggtcg
     1621 gcggaagttc agccaaaaat aagagatcga tgcctgatgt acaaccatgt cgcatccgac
     1681 aaggcatcag atcccaattc attcctggag caggagtcat gtcatgagta aacttttgga
     1741 tcgctttcgc tacttcaaac aaaagggcga aacctttgcc gatggtcacg gacaggtgat
     1801 gcatagcaac cgcgactggg aggacagcta tcgccagcgt tggcagttcg acaaaatcgt
     1861 gcgttccacc cacggcgtta actgtacagg ctcctgtagc tggaaaatct acgttaaaaa
     1921 tggtctggtg acctgggaaa tccaacagac cgactacccg cgcactcgcc ctgacctgcc
     1981 caatcatgaa cctcgcggct gcccgcgtgg cgcaagttac tcctggtatc tttacagcgc
     2041 taaccgcctg aaatacccgc tcattcgtaa acgactgatt gaactgtggc gcgaagccct
     2101 caaacaacac agcgatccgg tactggcgtg ggcatcgatt atgaacgatc cgcaaaagtg
     2161 cctgagctac aaacaagtgc gtgggcgcgg cgggtttatc cgctccaact ggcaggaact
     2221 aaaccagctg attgccgccg ctaacgtctg gaccatcaaa acctacggcc cggatcgcgt
     2281 tgccggtttc tcgccgatcc cggcgatgtc gatggtttct tacgccgccg gaacgcgtta
     2341 tctgtcgctg cttggcggca cctgttaaag tttctacgac tggtattgcg acctgccgcc
     2401 cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg gaatctgccg actggtataa
     2461 ctccagctac atcatcgctt gggggtctaa cgtaccgcag acacgtacgc cggacgccca
     2521 cttctttacc gaagtacgct acaaaggcac taaaaccatc gccattaccc ctgactactc
     2581 tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa caaggcactg atagcgccct
     2641 ggcgatggca atggggcatg tgattttaaa agagtttcat ctcgataatc ccagcgacta
     2701 ctttatcaac tactgccgcc gctacagcga catgccgatg ctggtaatgc tggagcctcg
     2761 cgacgatggt agctacgttc ccgggcgcat gatccgcgca tctgacctgg tggatggact
     2821 gggcgaaagc aacaatccgc agtggaaaac cgtagcagtt aataccgcag gtgaattggt
     2881 agtgccgaac ggttcgattg gtttccgctg gggagaaaaa ggcaaatgga atctggaatc
     2941 cattgccgcc ggtacggaaa ccgaattgtc gttaaccctg ctcggtcaac atgacgctgt
     3001 tgcaggcgtg gccttcccct actttggcgg cattgaaaat ccgcattttc gcagcgtaaa
     3061 acacaatccg gtgctggtgc gccaattgcc cgttaaaaac ctgacgttag tcgatggcaa
     3121 cacctgtccg gtggtcagcg tttatgattt ggtactggcg aattacggcc tcgatcgcgg
     3181 gctggaagat gaaaacagcg cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg
     3241 gggtgagcaa attaccggcg taccgcgcca gtatattgaa accatcgccc gcgaatttgc
     3301 cgataccgcc cataaaacgc atgggcgctc aatgattatc ctcggcgcag gtgttaacca
     3361 ctggtatcac atggacatga actaccgcgg gatgatcaat atgctgatct tctgcggttg
     3421 tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggc caggaaaaac tgcgcccaca
     3481 aaccggctgg ttgccactgg cctttgcgct cgactggaac cgaccaccgc gccagatgaa
     3541 cagcacctcg tttttctaca atcattccag ccaatggcgc tatgaaaaag tctctgctca
     3601 ggagttactt tcaccgctcg ccgatgccag taagtacagc ggtcatctga ttgatttcaa
     3661 cgttcgcgcc gaacgtatgg gctggctacc ttctgcgccg cagctggggc gtaacccgct
     3721 cgggattaaa gctgaagccg acaaggccgg attatccccc acagaattta ccgcccaggc
     3781 gctgaaatcg ggcgatttac gtatggcctg cgaacaacca gatagcggca gcaatcatcc
     3841 gcgtaatttg tttgtctggc gctctaacct gcttggctcc tccggcaaag gccacgagta
     3901 tatgcagaag tatctgctgg ggaccgaaag cgggattcag ggcgaggaac tcggtgccag
     3961 cgacgggatc aaaccggaag aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga
     4021 cctgctggtg acgctcgact tccgcatgtc cagtacctgc ctgttctccg atatcgttct
     4081 gcccaccgcc acctggtatg aaaaagacga tatgaacacc tcggatatgc atccatttat
     4141 tcatccgctt tctgcggcgg tcgatccggc ctgggaatca cgcagcgact gggaaatcta
     4201 caaaggtatt gccaaagcat tttcgcaagt gtgcgtgggc catcttggca aagaaaccga
     4261 cgtggtatta caaccactgc tgcatgactc tccggcagag ctctcacagc cgtgtgaagt
     4321 gctcgactgg cgcaaaggcg aatgcgatct gatcccgggt aaaaccgcgc cgaatattgt
     4381 ggcggtggag cgcgactacc ctgctacgta tgaacgcttt acctcgctcg ggccattgat
     4441 ggacaaactt ggcaatggcg gtaaagggat ttcgtggaat acccaggatg aaatcgattt
     4501 cctcggcaaa ctcaattaca ccaagcgtga tggcccggca caggggcgtc cgctgattga
     4561 caccgccatt gacgcttcag aagtgattct ggcactggca ccggaaacca acggtcatgt
     4621 tgcagtcaaa gcgtggcagg cgctgggcga gatcaccggg cgcgaacata cccatctggc
     4681 gctgcacaaa gaggacgaga agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat
     4741 tatctccagc cccacatggt ccggtctgga aagcgatcac gtatcctata acgcgggata
     4801 caccaacgtt catgagttaa ttccgtggcg cacgctgtcg ggacgccagc agctctatca
     4861 ggatcatccg tggatgcgtg cttttggtga aagcctggtg gcttatcgcc cgcctatcga
     4921 cacccgtagc gtcagtgaga tgcgccagat accgccaaac ggcttccagg aaaaagcact
     4981 taacttcctg acgccgcacc agaaatgggg cattcactca acctacagtg aaaacctgct
     5041 aatgctgacg ctctctcgcg gtggaccgat tgtctggatc agcgaaacag atgcccgaga
     5101 actgaccatt gtcgataacg actgggtgga agtatttaac gccaacggcg cgctgactgc
     5161 ccgcgcggtg gtcagccaac gtgtaccgcc gggcatgacc atgatgtatc acgcccagga
     5221 acgcattatg aatattcctg gttcggaagt aactggcatg cgcggcggca ttcataactc
     5281 ggtcacccgc gtttgcccga aaccgacgca tatgattggc ggttacgcgc agctggcctg
     5341 gggctttaac tactacggca ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa
     5401 gatgaagaac gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa
     5461 atgaaaatac gttcgcaagt cggcatggtg cttaacctcg ataaatgtat cggctgccat
     5521 acctgttcgg tgacctgtaa caacgtctgg accggacgcg aaggcatgga gtacgcatgg
     5581 tttaacaacg tcgaaaccaa accgggcatt ggttatccga aaaactggga agatcaggaa
     5641 gagtggcaag gcggctgggt ccgcgatgtg aatggcaaga tccgtccgcg tctgggcagc
     5701 aagatgggcg taataaccaa aatcttcgcc aacccggtgg tgccgcagca gattgatgat
     5761 tactacgaac ctttcacctt cgactacgaa catttgcata gcacaccgga aggcaaacat
     5821 atccctactg ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa agtgatctgg
     5881 ggcccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5941 ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa acaccttcat gatgtacctg
     6001 ccgcgcctgt gcgaacactg cctcaatccc agctgcgtgg cgacctgccc aagcggcgct
     6061 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     6121 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     6181 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     6241 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatc
     6301 gaggaagcgt cgagcaccga gcgcgaagtt gacct
//
LOCUS       DAJVRL010000160         6331 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVRL010000160 DAJVRL010000000
VERSION     DAJVRL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.161"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            5..436
                     /locus_tag="OQN02_003680"
     CDS             5..436
                     /locus_tag="OQN02_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS1644323.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OQN02_003681"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OQN02_003681"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1644324.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OQN02_003682"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OQN02_003682"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1644325.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OQN02_003683"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OQN02_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1644326.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OQN02_003684"
     CDS             complement(4438..5145)
                     /locus_tag="OQN02_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1644327.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OQN02_003685"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OQN02_003685"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1644328.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OQN02_003686"
     CDS             complement(6003..6206)
                     /locus_tag="OQN02_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1644329.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1405 a         1810 c         1648 g         1468 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc caccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct t
//
LOCUS       DAJVRL010000161         6312 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVRL010000161 DAJVRL010000000
VERSION     DAJVRL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.162"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(148..1194)
                     /locus_tag="OQN02_003687"
     CDS             complement(148..1194)
                     /locus_tag="OQN02_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCS1644330.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(1205..2190)
                     /gene="tssG"
                     /locus_tag="OQN02_003688"
                     /pseudo
     CDS             complement(1205..2190)
                     /gene="tssG"
                     /locus_tag="OQN02_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(2187..4070)
                     /gene="tssF"
                     /locus_tag="OQN02_003689"
     CDS             complement(2187..4070)
                     /gene="tssF"
                     /locus_tag="OQN02_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS1644331.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(4086..4580)
                     /gene="tssE"
                     /locus_tag="OQN02_003690"
     CDS             complement(4086..4580)
                     /gene="tssE"
                     /locus_tag="OQN02_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS1644332.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(4577..5407)
                     /locus_tag="OQN02_003691"
                     /pseudo
     CDS             complement(4577..5407)
                     /locus_tag="OQN02_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(5394..6308)
                     /gene="tagK"
                     /locus_tag="OQN02_003692"
                     /pseudo
     CDS             complement(5394..6308)
                     /gene="tagK"
                     /locus_tag="OQN02_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         1322 a         1748 c         1859 g         1383 t
ORIGIN      
        1 tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt aatgattcgg gctttcgtcg
       61 ttttggtgaa gtgagaattt ttcactccga agtaaaacgg atatgattta aaaaatggcc
      121 agacattata aattctggcc acttctgtta ctccagcgca tcccatttct ccagcagttc
      181 tactggaatt tcatttatta cctccgagac gcgttttccg accaggcgtt ctgcctggcg
      241 cagcaaagga atggtcgggc tgctcggttc gctgtgctca aaccagcgac ggatgacacg
      301 caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg ggttctgcgc cggtcatcgg
      361 tgatatttcc tccgcgatat gcaccggtag cgcaatctgc tctgcctgca tctgttcagt
      421 atgaacgatg gtggcgtttt ccggctgctc agcctgtggt tgtggcagga tctccggcgt
      481 ggtgctctgc actgctcccg gtaacagggc cagcagttgc gtcagacggg agaagtccgg
      541 tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc cgctcagtgg tctctgctgc
      601 ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc aggtctgcca gttgctggcg
      661 taccgattct ggtgccagcg catccgccgg gcgtaatgca gatagcgaac gctccacatc
      721 ccgcacctga aggcgcatgg cagcattatt tgacagtgta atgccacgaa tatccgccat
      781 tacaccttcg tggtccagca acgccgccag cgcgttactg cgtgccaccg cagcatcttc
      841 ggcggtgata tcttcagtcg cgagcagttg aggatggatg gcgtccgggt acgtggagca
      901 caacgtttcc agcagcgtga gcgcctccga cagtcccctg gcaccggcct gctggatacg
      961 gcagcgcagc aggacaacca gaatgcggat atctttgctg cgcatcagca gacgatgggc
     1021 gtcccgctca agctccgccc agttaatgtt ttccggcgta ctgacaaaat cgccatattg
     1081 cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg tattccggat cgtactccag
     1141 atccgggccg caggggcatt ccgccgaaat tgggtcagac aggaatattt ccatttaatt
     1201 atcctcagtg cgccaggtac tgttctggtt cgaaaatcat gcccgtcacc gggttgtcgt
     1261 gcatggcttt acccgcccat gcagtccaac ccagttggta cggaccaccg gtaacagccg
     1321 gaggggcggc gtggggcttt agctgtagct caatttccca gcagtattca taaccactaa
     1381 aggcgcggac cagttctgtc aggacgggca ggttattgct gccaggcaga aaacgtcggt
     1441 aatcctccag cgtcagcggg ccgataacca gccggaactt atactgcatg tccggcacgg
     1501 cctggccgat gagtgcacca ttgccaataa cggaagaggg ggcaggcatc cccagccggg
     1561 tgatctcatc gtccgccacg gctatccagt gcaggacata ctcctgtatc cggaaaggta
     1621 caccgaaata gtgcgccagc gtggtggcca gaccgtccgg attgcgggac tcacgcacca
     1681 gatgggccga tgccgccaga cgagcgtgat ctggcaacgg gctgtcagcg ctctcccgca
     1741 aatcctgccc gctgaggctg gcgacataaa aggcaaagcg gtcatgttgc ggtttatcca
     1801 gaccgccccc agcggactgg gcgctgcgcc atgcctggta aaactgcgtc agccagcggt
     1861 gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca gtccggttac gcgccagttc
     1921 ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc ccgaggctgt acagactgat
     1981 gtgcaggcgc ccttcctgcc agcccacact ggcgatttct cgtggcgcaa aggtcagcgc
     2041 gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc caggtgttgc ccgccggaac
     2101 ggtacacaac tgcacatcca cgcggcgcat caggttcaga aaaccgtaac gccagggggg
     2161 ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc gctgtcccgc cctgacaggc
     2221 caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca tctgcgagaa ggtattaatg
     2281 gaaacatggc gggcgatata gtgttccagc accaggccga acagatacgg actgatgccg
     2341 gaaaaacctt cttcatccac cgtcagttca caactgacgc cacgcccata gaccagtagt
     2401 ccggaaccgg gcagacggcg ggtgaccggg gtcgttttac aaccaatcag gctgcgcacc
     2461 tggcgcgatt gcggactgtc atgagcagga ataaacaggt tcagcagatc tcgcaacgcc
     2521 tggccgccgg tacggtgatc cagatcggcc agcggcagat aattaaaaga taactggcgg
     2581 atcaaccgcc atgccatctc acgttcagcc agcgggggct gcggcgagcg tggcgggcgg
     2641 ataagcccta cgcccgccac cgggatggct gcatccacgg ttagatcgtc ccggccattg
     2701 cgggcaataa gacagggcag atcgcggtta gtgaccatgg ccgtgatggt gatgtggcgc
     2761 agattttccg ggtacggcgc ttcatactgg tcaaccagcg acaggaacac ttccgagccc
     2821 gtataggggg tacgggtgcc gtaacggcgg gcgttttctg ataagcggcg gggctcccgg
     2881 cgcagggaaa aataacgtcc gtgattgcct tcatcattat tgcgggtgtg gtagagcggg
     2941 cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt ccacttcctg gaccgagaac
     3001 acctcataat ccagcggatg ggttctgtcc accaccagat gctgttccgt cgtgctgtgc
     3061 gtgacatcga tccgggcggt gacgcgggga aacaggttga taaccggggt acagaacagg
     3121 ctgaactgtg ccgcatttgt ctggtggatc agccagtccg gcggcaggcg gttcagcagg
     3181 ataacaatct ccgccacgcc accgtcaatt ttctgtaatc cggcagacag gcctgtcggg
     3241 gtgaaaaaat agaagcgttc cggacaggca aaatattcat gcagcaggtt atgaccgtga
     3301 aacacgttcc acgccagcgg cagcagcccc tgatccggct ccaggccttc gtacataacg
     3361 ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc ccggaatgcc agccagggtc
     3421 gcgatggcgc tggtatgtag aagttctaac aggtgtgagg ccgtacgctc ctcgccgcag
     3481 agataaaacg gcagcctgtc cagtccggca agctgactga acgttagttc accaaaggtg
     3541 cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg ccggcaggta gcggtgcagg
     3601 gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt cgatggcgag cggccacagc
     3661 gttacatcct ggctgctgcg aaactggcag gcagtgcttt cgccttccgg aatggcggag
     3721 aaaaaggctg tgtcgcgcgg gacggtaaat cctttcgcca gatcgccttc ttccgtatcg
     3781 ggatgcaact gcgccacgct cattgagggc gtgggtgtga cgtaattggg gctgaccact
     3841 tccagcaggc gctgggtgaa acgggggaat tcagcgtcaa tttttagctg cgtgcgggcg
     3901 gtcaggaagc tgaaggcctc gaccattcgc tctacatacg gatcggcaat atccgttccc
     3961 tgcatgccca gccgggcggc gactttggga tgacgggcgg caaattcggc tcccgtttcc
     4021 cgcagatagc tcagttcgcg gttgtagtat tccagtagcc gtggatccat cggtctctct
     4081 ctgaattaaa aaaacgtaat gcgactctgc tccatatcca gcgcgctacg tacccggaat
     4141 gcagtcggat acggctgcgt gaggatctcg ccgcgaattt caaactgtaa tgtgttatgg
     4201 ccactctggc ggcgctcatc ctgaagtggg atgatttgca gagtggacgc tttcagccgg
     4261 ggttcaaacc gggtgatggt tcgcaaaatg gccctgcgga tatcgtccca cttgtgctca
     4321 tacagaaagc tgcccgccag tggcggcagg ccgtagttaa gtacggacgc tgccgcgtac
     4381 ggataacgtg cagcatcaat atcatcttca tggctgacgg tgttgagcag aaatgacaaa
     4441 tcccggcgga tgatctcctt aatctgaacc ggactgacgc tgataccgtg gtcgcgtttc
     4501 tggtttgggg cgtcgtcaca caggcggtca aacagcgtgg gcagcaggtg attgcctgga
     4561 cgctggcggg aagcgctcac gcgtcgcttc cttcagcggt gttaaaggta caggtggaca
     4621 aatccagcag gctgatatcg ccgtgaccgg tgagccacac cctctgaccc agcgcctgta
     4681 ccagtgtctc tcccggaccg tcctgccaga ccgtttcccg gcacagacgc agggagtcgg
     4741 cagccttttc agagccgctg tagcggggaa acagctaggc actgtgtgta tcgccattgt
     4801 tcagggtgac gttgacgggt ttccagatca aatcgatcag atgcgcaggc cgcggcgatt
     4861 tcagcgaaca gatttgtgaa aacggcagcc agatatatgc tccgcccgcg atcagttcga
     4921 gtaccggacc ggtacgggag tcgctgtcgc taatccagtc aaatgcgccg ccattccact
     4981 gcccggaagt gtctgttatg gcctcaagcg cgcggctgcg gtgagcatca gcttcctgcg
     5041 cttcaccacg ggcgttacag gcgagggcgg tcagcagatc ctccatccag gcgggcggcg
     5101 ggagaataac accgggccgc tgttcgccct gaaagcaggc gtggcggtat atctcacagc
     5161 ggatcagctc gccaaatacc tgcgcttcgc gagtataatt tgcgtccatc cgggcgcata
     5221 gctggatctg ttgcagtgcc cgcgaccagt cgcctgccac gcacaataac tgaaacaggc
     5281 tgtggcggcg ggaggcctgc gctgggtgca tcctgacctg ttgctctgcc atctgaatgc
     5341 cctccaccac ggaataatcc cgcagcagcg ccgacaaggt aatcggtagc tcgttattgt
     5401 ttgtcatgat atatctctcc atgttgtgcc gggtttatcc ggtaatggct gtcaatgcca
     5461 atgatgcgat gctctttccg tgtcagatct ggcagtacgg tgttatgcgc tgtttccggg
     5521 gcgtattccg gggaaagtaa atgcaggata tcagggggag tcttttcgac aggccagata
     5581 gtttcatctt cgccagtggc atccagtaca ttgagaatag cgtcaatgct taacccacca
     5641 gtgaccatat cctgcaatgt ctcggcttcg gtgaccaggt cacgcaatga atccagatcc
     5701 tgcgaggagg gctcttcgtt aaaaggaaaa ggatcagcat ggcgattaag atcttgttcg
     5761 ggagataaca acgcctcgcg gtattcctga taaagtcggg tcagggcatt tcctttttcc
     5821 gcaccttctc cggtggagga ggctccatga acaggaatga tatcgaatgg attttgtgat
     5881 gtcagatgct gctgcctgaa ccaatccaga tccagatatt cggttattgc ttccgacgtg
     5941 atggcagtct gcgatatcgt ttttactgtg ctcaggttcg gagatgatgg attgccatgt
     6001 acaaggcact atgaagataa cccccattct atcaggtcgc cttcattcag acgcatacgg
     6061 tgatgaggtt ccacaatctg gtcgtttacc gtacagcaat attcggtaga ctgattgata
     6121 atccaccatg cttcttcgtg gcgggtcaga gtgagggcaa tctttacatc atcattcacc
     6181 ttaaaaacag gaatccaggg agaggtaacg gtgaagataa tttgtgttcc ttcagtcgcg
     6241 taaattgtgt tatcggcgat cccgtgcgcc tgtatttttt ggagtcccca cgtaatattt
     6301 ggtttcattg tg
//
LOCUS       DAJVRL010000162         6272 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVRL010000162 DAJVRL010000000
VERSION     DAJVRL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.163"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..666)
                     /locus_tag="OQN02_003693"
     CDS             complement(<1..666)
                     /locus_tag="OQN02_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCS1644333.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..2413)
                     /locus_tag="OQN02_003694"
     CDS             complement(656..2413)
                     /locus_tag="OQN02_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCS1644334.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2429..3313)
                     /locus_tag="OQN02_003695"
     CDS             complement(2429..3313)
                     /locus_tag="OQN02_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1644335.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3324..3929)
                     /locus_tag="OQN02_003696"
     CDS             complement(3324..3929)
                     /locus_tag="OQN02_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1644336.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4100..>6272)
                     /locus_tag="OQN02_003697"
     CDS             complement(4100..>6272)
                     /locus_tag="OQN02_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCS1644337.1"
                     /translation="DKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKL
                     IVLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDN
                     PSTIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYW
                     GLINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFE
                     GRDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQL
                     GYQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMII
                     QELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFK
                     LAEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKK
                     SPAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRK
                     NNPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLP
                     AVVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPD
                     RIWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLK
                     RDPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1836 a         1569 c         1316 g         1551 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg
      841 ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac
      901 ctgcgagtga gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa
      961 tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct
     1021 gttcaaaacg cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa
     1081 catttaactc actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg
     1141 catccagtac atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt
     1201 tggcgtggag atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc
     1261 gaataccgcg ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt
     1321 tactgccttg cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg
     1381 tactcattcc gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca
     1441 taaagcgata cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc
     1501 atcgatccgc tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt
     1561 tagcatataa gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt
     1621 cacccaatgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta
     1681 atctctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca
     1741 gaagttgcgt ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat
     1801 agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac
     1861 tcgccacccg ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa
     1921 atagaccacg cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt
     1981 taaccttaaa ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa
     2041 cccatgtggc aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc
     2101 cgacctggct gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca
     2161 taagagacgg gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg
     2221 cataaaaagc agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat
     2281 gttcatgccc gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc
     2341 agtgccgata atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc
     2401 gtgaattttc cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa
     2461 ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta
     2521 atagttttcc actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc
     2581 cgcaaaaacc actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg
     2641 gtgtcagcaa cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca
     2701 gaccttccag cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt
     2761 tcccccataa atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca
     2821 gtaacgcccc gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca
     2881 aggcaaaaac ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg
     2941 aggggtcacc cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa
     3001 ataacagaaa cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc
     3061 aatataacca acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa
     3121 aacttatcag cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa
     3181 aacaaatgac gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct
     3241 caggtaaccg ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag
     3301 tgttgccaga gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa
     3361 cgattaatcg cagtacagat aaacagaatt gaagcaatgc tccataaaag attattccat
     3421 tgcatccact gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga
     3481 tcacttttgc caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa
     3541 ccgcaaaact cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt
     3601 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca
     3661 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
     3721 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
     3781 gccaccaaca ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg
     3841 tgattcgctg taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga
     3901 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
     3961 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
     4021 atatcaaggt attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta
     4081 aaaattatta cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta
     4141 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
     4201 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc
     4261 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag
     4321 agggggtgtg gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
     4381 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
     4441 cggggtcgag tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt
     4501 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct
     4561 tgagaacaac agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt
     4621 cttccaaacg tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc
     4681 taatcagctc agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg
     4741 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca
     4801 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
     4861 gttgtaaagc agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg
     4921 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
     4981 acacacgtat tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt
     5041 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
     5101 acgctaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
     5161 aagtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
     5221 catcaataat ggattcgggc aataaccacg catctttata aagctcctga atgatcatat
     5281 cccaatctct tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa
     5341 gattcaacgg accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
     5401 gcggttcacg gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca
     5461 actccagaat ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat
     5521 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
     5581 gcattatggc aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt
     5641 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
     5701 cttttttcat ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt
     5761 ctttcagagc atcttcatca tttattagac cccaataata tgcatcagga tttattatca
     5821 tcagcgcttt tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac
     5881 tatttgtcga agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat
     5941 taatactccc cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac
     6001 ttgcatttat cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca
     6061 cgttaccgag aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg
     6121 tcaaattgcc aagaacaatc aatttttgat tactctctat attaccttct accactaaat
     6181 cgccacgatg cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac
     6241 tgttgatagc ttgttcaact tgttgtttat ct
//
LOCUS       DAJVRL010000163         6252 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVRL010000163 DAJVRL010000000
VERSION     DAJVRL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.164"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OQN02_003698"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OQN02_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1644338.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OQN02_003699"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OQN02_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1644339.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OQN02_003700"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OQN02_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OQN02_003701"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OQN02_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1656 a         1456 c         1476 g         1664 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag at
//
LOCUS       DAJVRL010000164         6077 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVRL010000164 DAJVRL010000000
VERSION     DAJVRL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.165"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            401..1429
                     /gene="torT"
                     /locus_tag="OQN02_003702"
     CDS             401..1429
                     /gene="torT"
                     /locus_tag="OQN02_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1644340.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1402..2094)
                     /gene="torR"
                     /locus_tag="OQN02_003703"
     CDS             complement(1402..2094)
                     /gene="torR"
                     /locus_tag="OQN02_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1644341.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2181..3353
                     /gene="torC"
                     /locus_tag="OQN02_003704"
     CDS             2181..3353
                     /gene="torC"
                     /locus_tag="OQN02_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1644342.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3353..5893
                     /gene="torA"
                     /locus_tag="OQN02_003705"
     CDS             3353..5893
                     /gene="torA"
                     /locus_tag="OQN02_003705"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCS1644343.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5890..>6077
                     /gene="torD"
                     /locus_tag="OQN02_003706"
     CDS             5890..>6077
                     /gene="torD"
                     /locus_tag="OQN02_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCS1644344.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1476 a         1519 c         1684 g         1398 t
ORIGIN      
        1 taagttggag tcattaccgt ccatttcggt cccagttagg acgctttctc atcaagccag
       61 tccgcccacc actgcatcat ctccctacgc ttatcgagat actgagcatg gttgtaaatt
      121 caattaaccc agtgcgttga caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta
      181 atgctctgtt acaggtcact aataccatct aagtggttga ttcatagtga ctggatatgt
      241 tgtgttttgt cgcgtttttc agtctattat ttaatcatta agtaatctaa tgctatgatt
      301 tttaaattat ttatgtgatt ttgtcatcct ttaggtgaaa aaggttgagt cgcaaagtgg
      361 aatgcatcta gcataaagcc ttattattga tgaggctatc atgcgcgtac tgctattttt
      421 acttctttcc cttttcatgt tgtcggcatt ttcggctgat aacctgttgc gctggcatga
      481 tgcgcagcat ttcacggtgc aagcctctat gccgcttaaa gccaaacgcg tatggaaact
      541 gtgcgcgctt tatcccagcc tgaaagattc atactggtta tcgttgaact atggtatgca
      601 ggaggctgct cgccgctacg gtgtggattt aaaagtgctg gaggcaggcg gctacagcca
      661 gttggctacc cagcaagcac aaatcgacca gtgtaaacag tggggcgcag aggccatttt
      721 gctcggtagt agcacgacgt catttcccga cctgcaaaag caggtagcaa atctgccggt
      781 gatcgaactg gtaaatgctc ttgatgatcc ccaggtgaaa agccgcgttg gtgtaccctg
      841 gtttcagatg ggctatcaac ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact
      901 gaacgtgctg ctgatgcccg gacccgataa cgccgggggc agtaaggaga tggtcgaggg
      961 ttttcgcgca gccattgccg gaagcccggt gcgtattgtt gatattgcgc ttggtgataa
     1021 cgatattgaa atccagcgca acctgttgca ggagatgctg gaacgccacc cagaaatcga
     1081 cgtcgttgcc ggaacggcca ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa
     1141 aacgccgctt accgtggtgt cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg
     1201 gggaagagtg attatggctg ccagcgatca aatggtctgg cagggggaac tggcagttga
     1261 gcaggccatc aggcaattac aggggcaatc ggtgtctgat aatgtcagcc caccgatttt
     1321 agttctgacg ccgaaaaatg ccgaccgaga acatatccgc cgctcgctgt caccgggggg
     1381 atttcgtccg gtctattttt atcagcacac atcagcggct aagaaataac cttcaccatg
     1441 ttgcgtcacc agtaaatccg cgctgagttt atgacgtaaa cgacgaatta acacatcgac
     1501 ggtgcgcagg tcagggtttt ccacccgacg cgcagaaagc atacgcagca gacgctcacg
     1561 gctgagaatt tcgcccggat tagtcacaaa tgccaccaac atttcatact ctgcgcgggt
     1621 cagtttaatc ggctcgccat cccgctccag cgtatggcgc gacacattca ggcaataacc
     1681 cgcaaagcga tagcagtagt cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg
     1741 ccagagcaga tttttcaccc gtactaccag ttcgcgcagt tccagcggtt tggtgacgta
     1801 atcgtctgcg cccatttcca gcccaacaat acggtcaatc cgatcgctgc gtccggtaac
     1861 cagaataatc cccaccgttg agcgttctcg cagggcgcgg gttaacatca ggccattttc
     1921 atcgggtaag ttgatatcca gcagaattaa atctaccggc tgattctgca taatttcccg
     1981 tagcccggca ccgctcgccg taacggaaac ggtatacccc tcctgagtga agtaggattg
     2041 taatcgcgcc tgggtaaccg gcttatcttc aacaataaca atgtgatgtg gcatcagagg
     2101 gttttactca ttctgttcat atctgttcat atttgctcat taagatcgct tcactaaacc
     2161 ataattctac aggggttatt atgcggaaac tctggaacgc gctacgccga cccagtgctc
     2221 gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat
     2281 tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta
     2341 tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc
     2401 gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac
     2461 tggaagcgag caacgacatt taccagacct ttattgccca ctccattgat acacctgaaa
     2521 aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca
     2581 actcggcaac ctgtcgctcc tgccataact acgatgcgat ggatcatgcc aggcagcatc
     2641 ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc
     2701 ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg
     2761 agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa
     2821 tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga
     2881 aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg
     2941 ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc
     3001 gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc gacaccaata
     3061 ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata
     3121 tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg
     3181 gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga
     3241 ttggctttac cagtctcgat aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga
     3301 atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa
     3361 taacgatctc tttcaggcat cacgtcggcg ttttctggca caacttggcg gcttaaccgt
     3421 cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc
     3481 gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg
     3541 cgcgatggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc
     3601 gtcgaaaatg atcgccggat tgccggatca cgtacacaac gcggcacgta ttcgttatcc
     3661 gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc agcgcggtga
     3721 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
     3781 acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
     3841 ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag
     3901 cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt
     3961 cggttcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa
     4021 aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc
     4081 ggatcacgat gtttatgaat attacgagca gctaaaagcg aaagtcgccg ccggtgaagt
     4141 tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
     4201 tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca
     4261 tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt
     4321 tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg
     4381 ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc
     4441 ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca
     4501 gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg
     4561 tggttttggc tggcactaca acggcgcagg cacgccgggg cgtaaaggcg ttattctgag
     4621 tggtttctcc ggctctacgt cgattccgcc tgttcacgac aacagtgact acaaaggcta
     4681 cagcagcact attccgattg cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat
     4741 caactggaac ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt ttgccggaac
     4801 taacccattc catcgccatc agcagatcaa ccgcattatt gaaggctggc gcaagctgga
     4861 aacggttatc gccatagata accagtggac ctcaacctgc cgctttgccg atatcgtgct
     4921 gcctgcgacc acgcagtttg agcgtaacga tctcgaccag tacggcaacc actccaaccg
     4981 tggcattatc gccatgaaac aggtggtgcc gccgcagttc gaggcgcgca acgactttga
     5041 tattttccgc gagctgtgcc gtcgctttaa tcgtgaagaa gcctttaccg aagggctgga
     5101 cgaaatgggc tggctgaaac gcatctggca ggaaggtgta caacaaggca aaggacgcgg
     5161 cgttcatctg ccagcgtttg atgacttctg gaataacaaa gagtacgtcg agtttgacca
     5221 tccgcagatg tttgttcgcc accaggcatt ccgcgaagat ccggatctcg aaccgctggg
     5281 cacgccgagt ggcctgattg agatctactc gaaaaccatc gccgatatga actacgacga
     5341 ttgtcagggg catccgatgt ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc
     5401 gcagaagtat ccgttgcatc tgcaatctgt gcatccggat ttccgacttc actcgcagct
     5461 atgtgagtcg gaaacgctgc gtcagcaata tacggtagcg ggtaaagagc cagtgttcat
     5521 taacccgcag gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa
     5581 cgctcgcggt caggtgttgg caggagcagt agtttctgac cgctatgcac ccggcgtggc
     5641 gcgaattcac gaaggggcat ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg
     5701 caaatacggt aaccccaacg tgctgaccat cgacatcggg acttcgcagc tggcgcaggc
     5761 ggccagtgcg cacactacgc tggtggaaat tgagaagtac aatggaacag tggagcaggt
     5821 gacggcgttt aacggccccg tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca
     5881 ggtgaaatca tgaccacgct gacagcacaa cagattgcct gtgtttacgc ctggctggcg
     5941 cagttgttct cccgtgaact ggacgatgaa caactgacgc aaatcgccag tgcgcagatg
     6001 gctgaatggt tttcgttgct gaaaagcgaa ccgccgctca ctgcggcggt gaatgatctg
     6061 gaaaacagta ttgccac
//
LOCUS       DAJVRL010000165         6052 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVRL010000165 DAJVRL010000000
VERSION     DAJVRL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.166"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            430..1338
                     /locus_tag="OQN02_003707"
     CDS             430..1338
                     /locus_tag="OQN02_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCS1644345.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OQN02_003708"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OQN02_003708"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1644346.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="OQN02_003709"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="OQN02_003709"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1644347.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OQN02_003710"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OQN02_003710"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1644348.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="OQN02_003711"
     CDS             complement(5673..>6052)
                     /locus_tag="OQN02_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644349.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1490 a         1575 c         1562 g         1425 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagtaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgtcaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 ccgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAJVRL010000166         6027 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVRL010000166 DAJVRL010000000
VERSION     DAJVRL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.167"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            86..861
                     /gene="exuR"
                     /locus_tag="OQN02_003712"
                     /pseudo
     CDS             86..861
                     /gene="exuR"
                     /locus_tag="OQN02_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
     gene            1206..1868
                     /locus_tag="OQN02_003713"
     CDS             1206..1868
                     /locus_tag="OQN02_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1644350.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1872..2255
                     /gene="mzrA"
                     /locus_tag="OQN02_003714"
     CDS             1872..2255
                     /gene="mzrA"
                     /locus_tag="OQN02_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1644351.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2402..2756
                     /locus_tag="OQN02_003715"
                     /pseudo
     CDS             2402..2756
                     /locus_tag="OQN02_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2794..3099
                     /locus_tag="OQN02_003716"
     CDS             2794..3099
                     /locus_tag="OQN02_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1644352.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3102..3506
                     /locus_tag="OQN02_003717"
     CDS             3102..3506
                     /locus_tag="OQN02_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1644353.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3496..3795
                     /locus_tag="OQN02_003718"
     CDS             3496..3795
                     /locus_tag="OQN02_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1644354.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3981..4277
                     /locus_tag="OQN02_003719"
     CDS             3981..4277
                     /locus_tag="OQN02_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1644355.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4347..5333
                     /gene="yqjG"
                     /locus_tag="OQN02_003720"
     CDS             4347..5333
                     /gene="yqjG"
                     /locus_tag="OQN02_003720"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS1644356.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5626..5994
                     /locus_tag="OQN02_003721"
     CDS             5626..5994
                     /locus_tag="OQN02_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1644357.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1524 a         1469 c         1519 g         1515 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttcacattca ggtagccctg
      541 gcgacgcaga actccgccct ggcggccatc gttgaaaaaa tgtggaccca gcgtagccat
      601 aacccgtact ggaaaaaact acacgaacac attgattccc gtaccgttga taactggtgt
      661 gatgaccacg atcaaattct caaggcgtta attcgcaaag atcctcatgc tgctaagctg
      721 gcaatgtggc agcatctgga aaacaccaaa atcatgttat ttaacgaaac cagcgacgat
      781 ttcgagttca atgccgaccg ctatctgttc gccgaaaacc cggtagtgca tctcgatacg
      841 gccaccagcg gcagcaaatg aaattcctgt ccgacaggcg ctgcaatgcg cctgtttgtc
      901 tcgtcggtaa gcgaaaagta taaagtgtca gcctgtgtaa atcctctcgc caccctcccc
      961 tgcaatcagc aaaatcagac tccacggaca tgtaattttg ataacgaaca acgttgacct
     1021 ttgttacaat tagattcaat ttgaatttat gcttttgaat gctttcttat ctcacgtttt
     1081 aacagggaat agttcaggct gtgttgatgt atcaaacccg cagaacatac caaaacagca
     1141 ataacattgc ggtagtgcat cttttaaaac cagcgtggcg ttaaccgatt caccaggaat
     1201 aatgaatgga acttttgacc caattgctgc aagccctgtg ggcgcaggat tttgaaaccc
     1261 tggccaatcc atcgatgatt ggcatgttgt attttgtctt gtttgtaatt ttgttccttg
     1321 aaaatggctt gcttccggcg gcctttttac cgggcgacag tttactggta ttggtcggcg
     1381 tgttgattgc gaaaggcgcg atgggttacc cgcaaacgat tctgctgctg accgttgccg
     1441 ccagcctcgg ttgctgggtc agctatattc aggggcgatg gctgggcaat acccgcaccg
     1501 tacaaaactg gctatctcat ttacccgcgc attatcatca acgcgcacac catctttttc
     1561 ataaacacgg tttatcggcg ctgttaattg gtcgctttat tgcatttgtc agaacactgc
     1621 tgccgacgat tgccgggtta tcagggctga ataacgcgcg ctttcagttc ttcaactgga
     1681 tgagcggtct gctgtgggta ttgatcctga caactctggg ttacatgctc ggcaaaacgc
     1741 cggtattttt aaagtacgag gaccagctga tgtcatgcct gatgctgctc ccggtggtgc
     1801 tgctggtgtt tggcctggca ggttctctgg tcgtgttatg gaaaaagaaa tatggaaatc
     1861 gggggtaagg gatgcaaata cctcgcatgt cgcttcgcca gctagcctgg tccggcactg
     1921 ttttacttct ggtcggcacg ctgttgctgg cctggtccgc ggttcgccag caagagtcta
     1981 cgctggcgat tcgtgccgtt catcaaggca caacgatgcc agacggtttt tcgatctggc
     2041 atcaccttga cgctcatggc attcctttca aaagtatcac ccccaaaaac gacactctgt
     2101 taattacatt tgactccagc gaccagagcg ccgccgcaaa agcggtcctc gacagaacat
     2161 tgccccatgg ctacatcatt gcgcagcagg acaataacag tcaggctatg cagtggctga
     2221 cccggttacg ggataactct catcgcttcg gataacttcc tgttatccga aacatatcac
     2281 tcactttggt gatttcaccg taactgtcta tgattaatga ggcggtggtt aacactaccg
     2341 cttcgttgtc tggacaatcg ttcctttgta ataggtccga atcacaatgg aaggttcaag
     2401 aatgaaatac cgcatcgctt tagctgtttc tctctttgct cttagtgccg gtagttatgc
     2461 cactaccctg tgtcaggaaa aggagcaaaa tatccttaag gagatcagct atgccgaaaa
     2521 acaccaaaac cagaatcgta ttgacggtct gaataaagcc ctgagtgaag tccgggccaa
     2581 ctgttcagat agccagctgc gtgccgatca tcacaagaaa atcgcaaagc agaaagatga
     2641 ggtggcggaa cgccagcaag atttagccga ggcgaagcaa aaaggcgatg ccgatagaac
     2701 ggaaactggc agaagcgcag gaagagctga aaaagctgga agcgcgcgac tactaactca
     2761 caatagtcac tacttactca cctggagaaa actatgtcga aagaacacac tacggaacat
     2821 ctgcgtgctg agttgaaatc cctttccgat acgctggaag aggtgcttag ctcatctggc
     2881 gagaagtcga aagaagagtt gagtaagatt cgtagcaaag cggagcaggc actgaaacag
     2941 agccgttatc gcctgggtga aaccggtgat gccatcgcca aacaaacccg tgtcgcggcg
     3001 gcgcgtgctg atgagtatgt gcgtgaaaat ccgtggaccg gcgtgggaat tggcgctgca
     3061 atcggtgtag tgctcggcgt tctgctgtcg cgtcgttaat tatggcggac actcatcacg
     3121 cgcaagggcc cggtaaaagc gttctgggca tcgggcagcg aattgtttcc atcatggttg
     3181 aaatggtgga aacacgtctg cggctggcgg tggtggagct ggaagaggaa aaagctaatc
     3241 tctttcaact tttactgatg ctgggcctga cgatgctttt cgctgcattt ggtcttatga
     3301 gcctgatggt gctaattatt tgggcggttg atccgcaata tcgcctgaat gcgatgattg
     3361 ccaccaccgt ggtgttgctg ctactggcac tgattggcgg tatctggacg ttacgtaaat
     3421 cgcgtaaatc caccttgctg cgccatacac gccatgagct ggcaaacgat cggcaactgc
     3481 tcgaggagga gtcccgtgag cagtaaagtc gaacgtgaac gacgtaaggc gcaactgctt
     3541 agccagatcc agcaacaacg gctggatctt tccgccagtc gtcgtgaatg gctggaggca
     3601 acaggcgctt acgatcgtcg ctggaatatg ctgctaagtc tgcgctcctg ggcgctggtt
     3661 ggcagtagcg tgatggcgat ctggacgatt cgccatccta atatgctggt ccgctgggcc
     3721 agacgcggtt ttggcgtatg gagcgcctgg cgtctggtta aaacgaccct caagcagcaa
     3781 cagcttcgcg gttaacttcc ctctggccgg agcaattccg gccttatccc tcaaattttt
     3841 tgaatatttt tgacagtttt ccttgctaac aatcatcatt caccacgttt atgattctct
     3901 ccatcgacag caacgacgct aatagcgcgc ccttgcacaa aaaaacaatc agcagcctga
     3961 gtggcgcagt ggagagtatg atgaaaaaat tagaagatgt tggtgtactg gtagcgcaca
     4021 ttttaatgcc gattctgttt attaccgcag gctggggaaa aatcaccgct tactctggta
     4081 cccaacaata tatggaagca atgggtgtcc cgggttttat gctgctgacg gcatttttat
     4141 ttcacagcaa ctttgctgaa ggcgtcaact cgctgatgtt catgaaaaac ctgacaattt
     4201 ctggcggatt cctgctgctg gcaattaccg gtccgggcgc gtatagcatc gaccgcctgc
     4261 tgaataaaaa gtggtaagca cgcgctatac ttaacgataa aaaacgagga ggaagctcct
     4321 cgtttttgct attggaggag agaaaaatgg gtcaactgat tgacggcgtc tggcatgaca
     4381 cctggtacga taccaaatct accggcggta aatttcaacg ttcagcttcc gcatttcgta
     4441 actggctcac tgccgatggc gcacctggcc ccactggcac aggcggtttt atcgcagaga
     4501 aagatcgtta tcatctctat gtttcactcg cctgcccgtg ggcgcaccgc acgctgatca
     4561 tgcgcaaact caaaggactg gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc
     4621 tggaaaacgg ctggaccttt gatgacagtt ttccgggagc aaccggagat acgctctatc
     4681 aacatgaatt tctgtatcag ctttatctcc acgccgatcc acactacagc ggacgagtta
     4741 ctgttcccgt gctgtgggac aaaaagaacc acaccatcgt cagcaacgaa tcagcagaaa
     4801 tcatccgcat gttcaatacc gcgtttgatg cgctgggcgc aaaagcgggt gattactacc
     4861 caccagccct gcaaacgaaa attgacgaac ttaacggctg gatttatgac accgttaaca
     4921 acggcgtgta taaagctggt tttgccacca gccagcaagc ctacgacgag gcggtggcga
     4981 aagtgtttga atcgctggcg cgactggagc agattttagg ccagcatcgt tacctgaccg
     5041 gcaaccagct aaccgaagcc gatattcgcc tgtggactac gctggtgcgt tttgatccag
     5101 tgtatgtgac ccacttcaag tgtgataagc accgcatcag cgattacctg aatctgtatg
     5161 gcttcctgcg cgatatctac cagatgccgg ggatcgccga aactgtcaat ttcgatcaca
     5221 tccgtaatca ttacttccgc agccataaga ccatcaaccc tacggggatt atttcaattg
     5281 gtccgtggca ggatctcgat gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg
     5341 caccttacga ggtgcctctg ataattagct aagtgcatgt tgttcatgtc ggatgcggcg
     5401 tgaacgcctt atcaaaccta caaagcgttt atattcaata tgttacaatg accgcgtagg
     5461 ccagataagc gtagcgcatc tggcgatttt gcgcttgcca tcagtcttag cgcgccttca
     5521 cggcacttta ttaattcctt gaacgaatat ttactgccat ttcattcacg tctattctta
     5581 atttgctgct ttaaaaacaa gtgattgagc aaaattgagg caaaaatgga ctggtatctg
     5641 aaagtactaa aaaattatgt cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg
     5701 tttattctgg tcaacatcat ctttacgttc gtgctggggc tgctggataa aatgttaggc
     5761 tggcaacgcg ctggtggcga aggcatcctg acgataatct acggtattct ggtgttttta
     5821 ccgtggtggg cggttcagtt ccgccgcctg cacgacaccg accgctcggc gtggtgggca
     5881 ctgctattct taatcccgtt tatcggctgg cttattatca tcgtctttaa ctgccaggcg
     5941 ggtacgccag gcgaaaaccg ctttggacca gatccaaaac tggaacaaga ataatgatta
     6001 agcaccttgt aaaaggtgct tcaaatt
//
LOCUS       DAJVRL010000167         6015 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVRL010000167 DAJVRL010000000
VERSION     DAJVRL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.168"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="OQN02_003722"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="OQN02_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS1644358.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="OQN02_003723"
     CDS             578..1027
                     /locus_tag="OQN02_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1644359.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="OQN02_003724"
     CDS             complement(1028..1261)
                     /locus_tag="OQN02_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCS1644360.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="OQN02_003725"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="OQN02_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS1644361.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="OQN02_003726"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="OQN02_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS1644362.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="OQN02_003727"
     CDS             complement(2212..3021)
                     /locus_tag="OQN02_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCS1644363.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="OQN02_003728"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="OQN02_003728"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCS1644364.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVNTGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="OQN02_003729"
     CDS             complement(4677..5471)
                     /locus_tag="OQN02_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1644365.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="OQN02_003730"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="OQN02_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1565 a         1528 c         1346 g         1576 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt atatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcaac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagagacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgttcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgct aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
     5941 aagttcctga gtcgtgaccc gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc
     6001 cgggccattg cgggg
//
LOCUS       DAJVRL010000168         5985 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVRL010000168 DAJVRL010000000
VERSION     DAJVRL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.169"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..263)
                     /locus_tag="OQN02_003731"
     CDS             complement(<1..263)
                     /locus_tag="OQN02_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCS1644366.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            484..912
                     /gene="uspG"
                     /locus_tag="OQN02_003732"
     CDS             484..912
                     /gene="uspG"
                     /locus_tag="OQN02_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1644367.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1033..2598)
                     /gene="ahpF"
                     /locus_tag="OQN02_003733"
     CDS             complement(1033..2598)
                     /gene="ahpF"
                     /locus_tag="OQN02_003733"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1644368.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2843..3406)
                     /gene="ahpC"
                     /locus_tag="OQN02_003734"
     CDS             complement(2843..3406)
                     /gene="ahpC"
                     /locus_tag="OQN02_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1644369.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3778..4524
                     /gene="dsbG"
                     /locus_tag="OQN02_003735"
     CDS             3778..4524
                     /gene="dsbG"
                     /locus_tag="OQN02_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1644370.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4734..5636
                     /locus_tag="OQN02_003736"
     CDS             4734..5636
                     /locus_tag="OQN02_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1644371.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5783..5985
                     /locus_tag="OQN02_003737"
                     /pseudo
     CDS             5783..5985
                     /locus_tag="OQN02_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1607 a         1441 c         1348 g         1589 t
ORIGIN      
        1 atgacgaacg gaattaccac ccggtcgcct ttttgcaaat tttttacgtc cttcccggtt
       61 tcaacgactt cccccataaa ttcatgacca aaaatatcgc catgtttaac ctggggtatt
      121 ttgcctcgat aaagatggag gtcagagcca cagatcgccg ttaccgtaat acgcagaata
      181 atatcatctg cctgttcaat gcccggatcg ggaacatttt ctacctgaac gtgatgtggg
      241 ccgtgataag tcaatgcttt catgcaacct ccagagacag ttcaaaaaag agtcagtctt
      301 taagggtagc tatcacagcg ggaaaacggg cggtaccgca ggttttggga gaattcctag
      361 tgttcagtga gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat
      421 atcagggata aatattataa tcatggagaa tgatttgttc atgattaaca gggagaaagg
      481 gttatgtata aggcaatcat tatgccagtt gatgtatttg aaatggaatt gagcgacaaa
      541 gctgttcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta
      601 ctacccgggt cagccagcct gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa
      661 gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca cttcaccatc
      721 gatccttccc gcattaaaca acatgtccgt tttggtagcg tgcgggatga agtcaatgag
      781 ttggcagaag aactgggggc tgatgttgta gttatcggtt ctcgcaaccc atcgatttcg
      841 acccatctgt taggttctaa cgcctcgagc gtgatccgcc acgccaatct gcctgtactg
      901 gtcgttcgct aacagataaa agtatttatg ccgccctgcc tggaacatcc cggcagggct
      961 ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa
     1021 tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc
     1081 tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc
     1141 cgctgcgaac acgcctttga cgttggtttc acatttcgca tcaatgataa tctcgcccat
     1201 gcggttacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac caatctggac
     1261 aaaaataccg gccagttcga tgttgtgaat attgccgctg acacgatctc gatattccag
     1321 accaacgact ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca gaataatgtc
     1381 gacgtttttc aggctgcgca gtttgtcctg cagaacctgg tcggctttca tttctggcgc
     1441 aaattccagc agcgttacgt gctcaacgat acccgccagg tcgattgccg cttccacgcc
     1501 ggagttaccg ccgccgataa ccgctacgcg tttgccttta aacagcggtc cgtcgcagtg
     1561 cgggcagtag gtcacgcctt tagtgcgata ctgatcttcg cccggaacgt tcatgttgcg
     1621 ccattttgca cccgtcgcca cgataatgct gcgtgctttc agtaccgcgc cagaagctgt
     1681 ttcaatctgg tgtagaccac cttcaaccgc tgccgggatc agtttgctgg cgctctggct
     1741 gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg ccaacttctg
     1801 cccttcagtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga ggatctgacc
     1861 accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt aaattgctgc
     1921 cgctgcaccc gccgggccgg aaccaacgat taatacgtca taagcatcac gcttgttcag
     1981 ctcttctgcc gcacgttttt ccgcgccagt atcaattttg gcaacgattt cagtcaacgt
     2041 catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc ccatcacgtt
     2101 gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg
     2161 gttcaatacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttat ggcaagagag
     2221 cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct ccagcagaga
     2281 ctgcgcttct ttcgacggat gaccaccggt ccacagcaac gccagtacca gcgaggtgaa
     2341 ctcgtggccc agcggggagc ctgcaaaacg tggtccctgg ttggaacctg ggttggtgat
     2401 caggaaagac ggcttacgca ctggcaagct gttatcttct ttaaaggtga ctttgtctga
     2461 cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc tgtcatccag
     2521 cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga gttgagtttt
     2581 catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc aagcggcctc
     2641 tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca
     2701 cccgaagaat taggtgaatt tccgaagtat ttcgcgtcag agcaaggcga ctagcctgtg
     2761 aatccccggg agcttacaca agtaagtgac cggggtgagc gggcgacgcc aacgccgcta
     2821 tggcgtgaaa gacgaaggaa atttagattt taccaaccag gtccagagac ggagccagag
     2881 ttgcttcacc ttctttccat ttagccgggc aaacttcacc tgggtgagaa gctacgtact
     2941 gagctgcttt gattttacgc agcagatcag acgcgtcacg gccaatgcct tcagcggtaa
     3001 cttcgattgc ctggatgata ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac
     3061 cttcatcttc acgcatgttg tcgaagttac gggtcagggc gccagtcggg tcgccgatca
     3121 tcgcatattt gattttagcg atggtttcag agctgctgtg ccatgctttg tgggtgaagt
     3181 gagtatcggt agatactgcg tatacgtcta cgcccagttt ctgcagttct tcgtagtggt
     3241 cagcaacgtc acccagttcg gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga
     3301 agacgctcca gcggccttcg gtatcttttt cggtgatttc gatgaattcg ccgtttttga
     3361 atgcctggtt tttaaaaggt ttaattttgg tgttaatcaa ggacatctat acttcctccg
     3421 tgttttcgat gagatgtaag gtaaccaatt tttgccgatt cggctaatgc gtttccatta
     3481 tcaaatcgat aagttttacc ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc
     3541 cagcggcgat aaaaagtaaa atggccccct ttccaggtga ccgtgtaatc tgaactaccc
     3601 tttggtatct tcagcgcctg cctgcaggct gaggtgttgc ccttacaaat gcaacaacga
     3661 catggattac aacaccctca taaacaaagg gcaatcacct gatctaagct cttacctatg
     3721 acagtgatag gttatgcctt ttactcgact tttgcactga ctgaaaagaa caaattaatg
     3781 ttaaaaaaga tacttttact ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct
     3841 tccccagtaa aagcgattga aaaacagggc attacaatca tcaaaacgtt cgatgccccc
     3901 ggaggaatga aaggttatct cggaaagtat caggatatgg gcgtcaccat ctacctgact
     3961 cccgatggta agcacgctat ctctggttac atgtacaacg agaaaggtga aaacctgagt
     4021 aacacactta tcgaaaaaga aatttacgca ccagccggac gcgaaatgtg gcaacggatg
     4081 gaacaatccc actggctcct cgacggtaaa aaagatgcgc cggtcattgt ctacgtcttc
     4141 tccgatccgt tctgcccata ttgtaaacag ttctggcagc aggcgcgccc gtgggtagat
     4201 tctggcaaag tgcaattaag aacattgttg gttggggtta tcaagccaga aagcccggcg
     4261 acagcagcgg caattcttgc ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc
     4321 tctggttgca agcttaagct aaacgtgcct gcaaacgtaa gtacagagca aatgaaagtg
     4381 ttaagtgaca atgagaaact gatggacgat ctgggggcaa atgtcacgcc ggctatctat
     4441 tacatgagta aggaaaatac gctgcaacag gccgtggggt tgcccgatca gaaaacgctt
     4501 aatatcatta tgggaaataa ataaaaggta attcatggat ggttgaacta tatccctgct
     4561 tttcaaccat cctaaaaaag catgaatttc tcgctctacc ctaagtggct gtgctatttt
     4621 agaaaaactt aatcctgagt taagcaaaaa aacataatca ataaaaatac atacgcaaaa
     4681 aacattgact aagtgaatat accatggaag aaaaatataa accggagtag tgtatggcca
     4741 atctctacga cttgaaaaag ttcgacttaa atcttcttgt catatttgag tgtatttacc
     4801 agcatttaag cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc
     4861 agtcgttaca acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag
     4921 gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag
     4981 gccttgaaca aacaattaat atagtcaata agtccgaact aaagaaaaac tttattatct
     5041 acggccctca gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag
     5101 atgcctctgt cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac
     5161 tgttggttca ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg
     5221 tcatttgcat gccattgcat acaattcgca atactctaat ttgtagtaat aagcatccgc
     5281 gaattactga caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatag
     5341 ccaaatcagc aggagttgat gatattcaaa tggaaataga cgagaagttt atgaatcgca
     5401 aaatttcatt tcgtggttcc tctttgctga caataattaa tagcattgca gttacagatt
     5461 tactgggaat agtcccctat gaactttata actaccaccg tgactttctt aatttaaaag
     5521 agataaaact agaacatccg ttaccttcaa taaagcttta catttcctat aataaatctt
     5581 ctttaaataa tttggtgttc tcccgattta ttgaccgtct aaatgagagt ttttgatata
     5641 taactataga cagctactat aactttcatc tatttattca cagcgcgaat gaaaaataaa
     5701 acactctgct ttacagtgtt tactttataa ttaacagtct tatctcactg ttcaccctgt
     5761 aacgttcaga gagcgtcttc agatgtctat ttataaaatt cctcttccgc tcaatattct
     5821 ggaagctgca cgagagcgta ttacctagac actcaacacc ctaccccgcg tttgtgtctc
     5881 attttctggc ggcaaagact ctggtttaat gctgcatctg acagccgaac ttgcccgaca
     5941 aatgggcaaa aaaatctgcg ttttgtttat caactgggag gcgca
//
LOCUS       DAJVRL010000169         5859 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVRL010000169 DAJVRL010000000
VERSION     DAJVRL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.170"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..312
                     /locus_tag="OQN02_003738"
     CDS             <1..312
                     /locus_tag="OQN02_003738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1644372.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(388..852)
                     /locus_tag="OQN02_003739"
     CDS             complement(388..852)
                     /locus_tag="OQN02_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1644373.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OQN02_003740"
     CDS             complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OQN02_003740"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1644374.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="OQN02_003741"
     CDS             complement(1721..2410)
                     /locus_tag="OQN02_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1644375.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OQN02_003742"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OQN02_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1644376.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OQN02_003743"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OQN02_003743"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1644377.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5855)
                     /gene="nanR"
                     /locus_tag="OQN02_003744"
     CDS             complement(5073..5855)
                     /gene="nanR"
                     /locus_tag="OQN02_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1644378.1"
                     /translation="MNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMIRRR
                     EFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADTIIG
                     ELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQSLD
                     NNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVSYQQ
                     HIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1443 a         1622 c         1495 g         1299 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgcggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatag tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataagg
//
LOCUS       DAJVRL010000170         5774 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVRL010000170 DAJVRL010000000
VERSION     DAJVRL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.171"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..931)
                     /gene="hprS"
                     /locus_tag="OQN02_003745"
     CDS             complement(<1..931)
                     /gene="hprS"
                     /locus_tag="OQN02_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1644379.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSL"
     gene            complement(931..1602)
                     /gene="hprR"
                     /locus_tag="OQN02_003746"
     CDS             complement(931..1602)
                     /gene="hprR"
                     /locus_tag="OQN02_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1644380.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1735..2148
                     /gene="hiuH"
                     /locus_tag="OQN02_003747"
     CDS             1735..2148
                     /gene="hiuH"
                     /locus_tag="OQN02_003747"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1644381.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2257..3261
                     /gene="msrP"
                     /locus_tag="OQN02_003748"
                     /pseudo
     CDS             2257..3261
                     /gene="msrP"
                     /locus_tag="OQN02_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3262..3897
                     /gene="msrQ"
                     /locus_tag="OQN02_003749"
     CDS             3262..3897
                     /gene="msrQ"
                     /locus_tag="OQN02_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1644382.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSPQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4155..4805
                     /gene="zinT"
                     /locus_tag="OQN02_003750"
     CDS             4155..4805
                     /gene="zinT"
                     /locus_tag="OQN02_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1644383.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1639 a         1306 c         1193 g         1636 t
ORIGIN      
        1 aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga aacagtatat
       61 tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt gttttttgat
      121 attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt aatgcattaa
      181 ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc tcaaaatctt
      241 tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt tctctcggta
      301 atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc gttacgccac
      361 tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat actgagcaca
      421 gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca agcatattat
      481 gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact tcatcaatgt
      541 taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg cctgctgcgc
      601 tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt gtccggttaa
      661 caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca tccatcatcc
      721 ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac tgcttgatct
      781 gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt gccaggccat
      841 tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata aaaagcaagg
      901 ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga atgaatagcc
      961 catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt ttgcgcggag
     1021 cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc cccaaatttc
     1081 actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta gccaaagtaa
     1141 ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac tttgactgac
     1201 agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat gttgccttaa
     1261 ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca gataatcatt
     1321 tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag taaggcaaat
     1381 aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat ccatacccgg
     1441 aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat aaagcccatc
     1501 tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct gcgttaccca
     1561 ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa tatgccataa
     1621 atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct gtaaggttac
     1681 cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat aaacatgtta
     1741 aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc tttggtttat
     1801 gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg aaaacccgct
     1861 gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca acttaatacc
     1921 gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac tgcgactact
     1981 ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa tcttgaaagt
     2041 ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca ttatcatgtg
     2101 cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg cgtaaatatg
     2161 taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa cagtaaacag
     2221 ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca atttttaaaa
     2281 gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt gttaaaagca
     2341 ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga tctgcttagc
     2401 tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga gttcagcaag
     2461 cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc cggttataac
     2521 aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg tagcctgaaa
     2581 accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac cctcgatcat
     2641 gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg ctgcgtggaa
     2701 gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact ggcgcttgcc
     2761 gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc ggaacagatg
     2821 cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt cgaaggattg
     2881 cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta tggcaaggcg
     2941 ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata tggctttaaa
     3001 gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac cacctggaat
     3061 ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt tgatcacccg
     3121 cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga tgttcagcgc
     3181 cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta tcgtggcctg
     3241 gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg gctgaaagtt
     3301 tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat caatcacggt
     3361 ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac tgcactgaaa
     3421 tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca gccgttattg
     3481 atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact gcatttaacc
     3541 agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa ggagttaatt
     3601 acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc tttagcgttc
     3661 acttcacccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt gcataacttc
     3721 gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa gattatttca
     3781 ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg gtataagaag
     3841 ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc tctataactt
     3901 catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc cctatccggc
     3961 ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca ccagaataat
     4021 ttattttatt tcaataagat agttcaatac acctgagaat accatgctct catttcctaa
     4081 tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt acattaactc
     4141 tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta ggtgtcttta
     4201 ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc ttaacagagg
     4261 tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac cgaacgctca
     4321 gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc aaacttgatc
     4381 ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa attaaagatt
     4441 attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac ggcattgtcg
     4501 aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga tacaaaatac
     4561 tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa gatcctgaaa
     4621 gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga aaatcatctc
     4681 attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa atggaaaact
     4741 ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag atgatgtctc
     4801 attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta gtaacaacaa
     4861 taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg agtcgtgtct
     4921 tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata cacaactcag
     4981 ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg gtaaagctaa
     5041 ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta tgatacaaag
     5101 gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt gtcccgcggc
     5161 tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct ctacgcaact
     5221 tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa agaataatgg
     5281 tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt cctgtcagct
     5341 ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga cgatctaacc
     5401 cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag tcgggagata
     5461 agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt tgcccctact
     5521 ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa tttttcttgg
     5581 aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt aaagaagaat
     5641 attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg tgatttgccc
     5701 cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt gttgaataat
     5761 tccactgcag ttat
//
LOCUS       DAJVRL010000171         5758 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVRL010000171 DAJVRL010000000
VERSION     DAJVRL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.172"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..402)
                     /locus_tag="OQN02_003751"
     CDS             complement(<1..402)
                     /locus_tag="OQN02_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HCS1644384.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(547..1050)
                     /gene="cheW"
                     /locus_tag="OQN02_003752"
     CDS             complement(547..1050)
                     /gene="cheW"
                     /locus_tag="OQN02_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCS1644385.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="OQN02_003753"
     CDS             complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="OQN02_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCS1644386.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3040..3966)
                     /gene="motB"
                     /locus_tag="OQN02_003754"
     CDS             complement(3040..3966)
                     /gene="motB"
                     /locus_tag="OQN02_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCS1644387.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3963..4850)
                     /gene="motA"
                     /locus_tag="OQN02_003755"
     CDS             complement(3963..4850)
                     /gene="motA"
                     /locus_tag="OQN02_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS1644388.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="OQN02_003756"
     CDS             complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="OQN02_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1644389.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5558..>5758)
                     /locus_tag="OQN02_003757"
     CDS             complement(5558..>5758)
                     /locus_tag="OQN02_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS1644390.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1341 a         1553 c         1407 g         1457 t
ORIGIN      
        1 taacgctgtg taatagtttt tatatttttc atcaaaatta cggctggtag cgaccatttc
       61 aggtaacggt gccatgcttt tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt
      121 cctagcgcta tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat
      181 catccgcacc gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga
      241 cgtcagctcg ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg
      301 gtgaagggaa gaaaaaaaca aactgccgga aataagctgt aacagtgcga atacccccag
      361 caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa
      421 caagttgatc tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc
      481 gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg
      541 ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag
      601 cagtttttcg atgttcacca gaatcaacat acggtcgccc agtgcgccca gtccagtgag
      661 atattctgtt gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa
      721 tgaaagcacg tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag
      781 gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa
      841 gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt
      901 gttcgcaatc cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat
      961 accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc
     1021 cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg
     1081 gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt
     1141 gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta
     1201 ctttcaaggt ttttaaccac aacctggtgt tgaccaatta attgatccac cagcaaggcg
     1261 tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg
     1321 cccgcgacgt tgaacacttt ccacagttcg acgatgggca gatattcacc ccgcacttcc
     1381 agcacccgct cgccgccggc cagtggatgg agatcggctt cacggggttg cagtgattcc
     1441 ataacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg
     1501 aggatggcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac
     1561 tggatttcga catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg
     1621 ccgcgcccgg agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc
     1681 attgcaactt cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt
     1741 gccagaattc gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg
     1801 ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc
     1861 cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg
     1921 tcgataatgc gttctatcag gctcttgtcg agctcggtgg aactgcccac cagcgtcagt
     1981 tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaag
     2041 acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta
     2101 cgttgtaact gccccatgct ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg
     2161 gaacgctggg caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga
     2221 tcaacctttt ctaccgctac acggatgctg gtggattcac tgctgcgcgt cgttttttcc
     2281 cgctccacgc ggccggttgg cgcttgttcg gctgccagtt taagcacagg tggggtagct
     2341 atttttggcg aaacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag
     2401 agtaccgctg tgatgtcatc ttcggcgata tcgcccggta atattgccga gagagaatcc
     2461 gcccctttca ccacgtcatt taacgttgtc agatgcccca gctcttcttc cagcaggtcg
     2521 acttcgccgg ccttcaggcg cgaaaggata attcgtcgcg gcaactgact gcgactttgc
     2581 tcatcttgcg gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg
     2641 cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgta gctggcggca
     2701 tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc
     2761 aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc
     2821 aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt tcctgcccct
     2881 cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc
     2941 ggctgcaaaa ccagcagatg ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa
     3001 aatgtctgat aaaaatcgct tatatcgatg ctcacgctgt cacctcggtt cggctgatgg
     3061 cattgtggga acactgacct gtggtgcaac ctcaggtttt tctagggcgc ttactggctc
     3121 attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccaa
     3181 caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc
     3241 cgccatgccg acgacacgta acactttgcc gctatccaac cctccgacca tcagttcgcg
     3301 gcgggatgca ttggcccgat cggcagaaag ctcccagttg ctgtaacctt tctcaccgct
     3361 ggcgtaggga aaatcatcgg tatgacctga aaggctaata cggttgggaa taccgttcag
     3421 cacaggcgcg atggcgcgca gaatgtcgcg catatagggt tcgacatcgg cactgccggt
     3481 tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag
     3541 atcgattttg agatggagac gtaacgcccg cagtttcgga tcggactcta tcaactggtc
     3601 gagatcaccc cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat
     3661 gttcggctgc ttattcactt ccccctggct ctgggtgtaa tcatcaccac cgccgggaat
     3721 tgggctttca ctattggaaa tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg
     3781 gaagtactcc gcaatctgaa tcaactcttt tgggctggag atggagatca gccacatcac
     3841 caaaaaaaag gccatcatcg cagtcataaa gtcggcataa gcaatcttcc acgatccatg
     3901 tgctgccccg tggcttttgg ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt
     3961 cttcatgctt cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg cacatgctct
     4021 tccagttcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg
     4081 gcgataggcg gtgcgtaacc gttcagatta gaaagcagag tgactttgac gcactgcatc
     4141 attttgctgg tttcggcgct tttctgacgt aaaacactcg ctagtgggga aataaatccg
     4201 taagccaata aaataccgag gaaagtcccc accatcgcat gtgcgataag cacccccagc
     4261 tcggcggcag gacgatcggc tgaacctaac gcgtgaacgc cccccattac agccgcgaca
     4321 ataccaaacg ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc
     4381 tcgctttcgt gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg
     4441 tgaccgctga taatcaggcg cagataatcg acgataaaat caagcatgac gctatccgcg
     4501 aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat atcacgttcc
     4561 agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga
     4621 tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgctttcagc
     4681 gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca
     4741 ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa
     4801 ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc
     4861 ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg attggggaaa
     4921 aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgccacaccg tatcagttaa
     4981 acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa
     5041 agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca
     5101 acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc
     5161 agtaatccac tttcaacaaa ccgcaccaat gtcctggcac gggttaatgc cagcagtggt
     5221 ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg
     5281 acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca
     5341 tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc
     5401 ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt
     5461 tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata
     5521 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
     5581 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
     5641 tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
     5701 caaaacggaa gtgacaaacc agctgattgg tttctgccag cttaaccatt tgcggaag
//
LOCUS       DAJVRL010000172         5702 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVRL010000172 DAJVRL010000000
VERSION     DAJVRL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.173"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..219)
                     /locus_tag="OQN02_003758"
     CDS             complement(<1..219)
                     /locus_tag="OQN02_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCS1644391.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="OQN02_003759"
     CDS             complement(216..1124)
                     /locus_tag="OQN02_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1644392.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="OQN02_003760"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="OQN02_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OQN02_003761"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OQN02_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="OQN02_003762"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="OQN02_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1644393.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="OQN02_003763"
     CDS             5533..>5702
                     /locus_tag="OQN02_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644394.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DAJVRL010000173         5692 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVRL010000173 DAJVRL010000000
VERSION     DAJVRL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.174"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(25..591)
                     /gene="satP"
                     /locus_tag="OQN02_003764"
     CDS             complement(25..591)
                     /gene="satP"
                     /locus_tag="OQN02_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1644395.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(740..1453)
                     /gene="msyB"
                     /locus_tag="OQN02_003765"
     CDS             complement(740..1453)
                     /gene="msyB"
                     /locus_tag="OQN02_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1644396.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1479..1883)
                     /locus_tag="OQN02_003766"
     CDS             complement(1479..1883)
                     /locus_tag="OQN02_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1644397.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2260..4176
                     /gene="dnaK"
                     /locus_tag="OQN02_003767"
     CDS             2260..4176
                     /gene="dnaK"
                     /locus_tag="OQN02_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1644398.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4265..5395
                     /gene="dnaJ"
                     /locus_tag="OQN02_003768"
     CDS             4265..5395
                     /gene="dnaJ"
                     /locus_tag="OQN02_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1644399.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5499..5651)
                     /gene="mokC"
                     /locus_tag="OQN02_003769"
     CDS             complement(5499..5651)
                     /gene="mokC"
                     /locus_tag="OQN02_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1644400.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1496 a         1412 c         1495 g         1289 t
ORIGIN      
        1 ccgagcgggg ggatctcaag acaattagtg ggattcacca atcggcagaa cggtgcgacc
       61 aaactgctcg ttcagcactt cacccatcgc cagatagatt gcgctggcac cgcagatcag
      121 accaatccag ccggcaaagt ggatgatcgc ggcattaccg gcaatgttac cgatcgccag
      181 cagggcaaac agcacggtca ggctaaagaa aacgaattgc aggacgcgcg cgcctttcag
      241 agtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga caccaaggaa
      301 ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg caaccagcgt
      361 cagccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta
      421 ctccagcaga ccagcaaaaa tttgcgcgat gccgccgtag aaaatgccca tggcaagaat
      481 aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc
      541 gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca taattcctca
      601 aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taactgtagt gttttcaggg
      661 cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa
      721 ttttccccgg tttccggtat cagacctgag tggcgctaac catccggcgc aggcaggcga
      781 tttgcagtac ggctggaatc gtcacgcgat aggtgctgcc gctgaccgct ttaaccccat
      841 ttaatgctgc gcccacaggg cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta
      901 cgctcattgc tgcgtgggtg cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca
      961 caccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc agctcattca
     1021 ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccatt ttcttccagg
     1081 tattacgcag aaattgttcc agtaactgtt gctcaatttc aaacgtagac atctctttgt
     1141 cggctttcag cttcaatcgc tttgaaacat cgagcaaaat ggcccgatac aatttaccgt
     1201 gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct ccggcaatca
     1261 gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac agttcgttgc
     1321 gcatcagtat gctggagagg cgagttttgc ctttttcatt atgggtgagc aatcgggcga
     1381 aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa tcattcaggt
     1441 aattaacatt cattttttgt ggcttctata ttctggcgtt agtcgtcgcc gataattttc
     1501 agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt tgagacgcag
     1561 cgtttattgt cgttatcgct gttaatgttg atccagtcag tggtttgccc ttcttttatt
     1621 tctgaaggaa tattcaggct ctgactggcg ctacgggcgg ctttgaaata aaccgatgca
     1681 ccacttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac aatgcgacaa
     1741 acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat gacgccgagg
     1801 agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc caggctggcg
     1861 gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag ggggatgaat
     1921 acccactagt ttactgctga taaagagaag attcaggcac gtaatctttt ctttttatta
     1981 caattttttg atgaacgcct tggctgcgat tcattcttta tatgaataaa attgctgtca
     2041 attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg
     2101 catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc agtgagtgag
     2161 tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat tacagactca
     2221 caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat aattggtatc
     2281 gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccattcc tcgcgtgctg
     2341 gagaacgccg aaggcgatcg caccacgcct tctatcattg cctataccca ggatggtgaa
     2401 actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa caccctgttt
     2461 gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg tgatgtttcc
     2521 atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga agttaaaggc
     2581 cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa gaaaaccgct
     2641 gaagattacc tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc atactttaac
     2701 gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga agtaaaacgt
     2761 atcatcaacg aaccgaccgc agctgcgttg gcttacggtc tggacaaagg cactggcaac
     2821 cgtactatcg cggtttatga cctggggggt ggtactttcg atatttctat tatcgaaatc
     2881 gacgaagttg acggcgaaaa aaccttcgaa gttctggcaa ccaacggtga tacccacctg
     2941 ggtggtgaag acttcgacag ccgtctgatc aactatctgg ttgaagaatt caagaaagat
     3001 cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa
     3061 aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc atacatcact
     3121 gcagacgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa actggaaagc
     3181 ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact gcaggatgct
     3241 ggcctgtccg tatctgatat cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca
     3301 atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg
     3361 gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg cgacgtaaaa
     3421 gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat gggcggtgtg
     3481 atgacgacgc tgatcgcgaa aaacaccact atcccgacca agcacagcca ggtgttctct
     3541 accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt
     3601 gcggctgata acaaatctct gggtcagttc aacctagatg gtatcaaccc ggcaccgcgc
     3661 ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatcct gcacgtttcc
     3721 gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc ttctggtctg
     3781 aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga agctgaccgt
     3841 aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca cagcacccgt
     3901 aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc tatcgagtct
     3961 gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa
     4021 atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc
     4081 cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc
     4141 gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaacg ggtaattata
     4201 ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa
     4261 aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag cggaagagcg
     4321 tgaaatcaga aaggcctaca aacgcctggc catgaaatac cacccggacc gtaaccaggg
     4381 tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag ttctgaccga
     4441 ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc aaggaggcat
     4501 gggcggcggt ggttttggcg gcggcgcaga cttcagcgat atttttggtg acgttttcgg
     4561 cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta
     4621 taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga tccgcattcc
     4681 gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta cacagccgca
     4741 gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat tcttcgctgt
     4801 acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa
     4861 atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg
     4921 ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc
     4981 accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt tcgagcgtga
     5041 aggcaacaac ctgtattgcg aagtcccgat caacttcgct atggcggcgc tgggtggcga
     5101 aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg aaacccagac
     5161 cggtaagcta ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg
     5221 tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaaa agcagaaaca
     5281 gctgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggcgagcaca acagcccgcg
     5341 ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc gctaacctcc
     5401 ccaaaagcct gcccgtgggc aggcctgggt aaaaataggg tgcgttgaag atatgcgagc
     5461 acctgtaaag tggcggggat cactccccgc cgttgctctt actcggattc gtaagccgtg
     5521 aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc ttttctcgtt
     5581 accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat catcgcctta
     5641 tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac tc
//
LOCUS       DAJVRL010000174         5414 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVRL010000174 DAJVRL010000000
VERSION     DAJVRL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.175"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..511
                     /locus_tag="OQN02_003770"
     CDS             <1..511
                     /locus_tag="OQN02_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCS1644401.1"
                     /translation="RGAYNEIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQ
                     TRLHLIANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDM
                     AAFEKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGM
                     DMSHAESAH"
     gene            533..2524
                     /gene="cyoB"
                     /locus_tag="OQN02_003771"
                     /pseudo
     CDS             533..2524
                     /gene="cyoB"
                     /locus_tag="OQN02_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2514..3128
                     /locus_tag="OQN02_003772"
     CDS             2514..3128
                     /locus_tag="OQN02_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1644402.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3128..3457
                     /locus_tag="OQN02_003773"
     CDS             3128..3457
                     /locus_tag="OQN02_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1644403.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3469..4359
                     /gene="cyoE"
                     /locus_tag="OQN02_003774"
     CDS             3469..4359
                     /gene="cyoE"
                     /locus_tag="OQN02_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1644404.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4544..4855
                     /locus_tag="OQN02_003775"
     CDS             4544..4855
                     /locus_tag="OQN02_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1644405.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4839..4997
                     /locus_tag="OQN02_003776"
     CDS             4839..4997
                     /locus_tag="OQN02_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1644406.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4990..5388
                     /locus_tag="OQN02_003777"
     CDS             4990..5388
                     /locus_tag="OQN02_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1644407.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1183 a         1346 c         1429 g         1456 t
ORIGIN      
        1 tcggggcgct tacaatgaaa tcgctttccc ggcgaacact ccggtgtact tcaaagtgac
       61 ctccaactcc gtgatgaact ccttcttcat tccgcgtctg ggtagccaga tttatgccat
      121 ggccggtatg cagactcgcc tgcatctgat cgccaacgaa cccggtactt atgacggtat
      181 ctccgccagc tacagcgggc cgggcttctc aggcatgaag ttcaaagcta ttgcaacacc
      241 ggatcgcgcc gaattcgacc aatgggtcgc aaaagcgaaa cagtcgccga ataccatgtc
      301 tgacatggct gcgttcgaaa aactggccgc gcctagcgaa tacaaccagg tggaatattt
      361 ctccaacgtg aaaccagact tgtttgccga tgtgattaac aagtttatgg ctcacggtaa
      421 gagcatggac atgacccagc cagaaggtga gcacagtgca cacgaaggta tggaaggcat
      481 ggacatgagc cacgcggaat ccgcccatta aaggggttga ggaagaataa agatgttcgg
      541 aaaattatca cttgatgcag tcccgttcca tgaaactatc gtcatggtta cgatcgctgg
      601 cattattttg ggaggtctgg cgctcgttgg cctgatcact tacttcggta agtggaccta
      661 cctgtggaaa gagtggctga cctccgtcga ccataaacgc ctcggtatca tgtatatcat
      721 cgtggcgatt gtgatgttgc tgcgtggttt tgctgacgcc attatgatgc gtagccagca
      781 ggctcttgcc tcggcgggtg aagcgggctt cctgccacct caccactacg atcagatctt
      841 caccgcgcac ggcgtgatta tgatcttctt cgtggcgatg cctttcgtta tcggtctgat
      901 gaacctggtg gttccgctgc agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa
      961 cttaagcttc tggtttactg ttgttggcgt gattctggtt aacgtttctc tcggcgtggg
     1021 cgaatttgcg cagaccggct ggctggccta tccaccgcta tcgggaatag agtacagtcc
     1081 gggagtcggt gttgattact ggatatggag tctccagcta tccggtatag gtacgacgct
     1141 taccggtatc aacttcttcg ttaccattct gaagatgcgc gcaccgggca tgaccatgtt
     1201 caagatacca gtatttacct aggcatcact gtgcgcgaac gtactgatta ttgcttcctt
     1261 cccaattctg acggttaccg tcgcgttgtt gaccctggat cgctatctgg gcacccattt
     1321 ctttaccaac gatatgggtg gcaacatgat gatgtacatc aacctgattt gggcctgggg
     1381 ccacccggaa gtttacatcc tgatcctgcc tgttttcggt gtgttctccg aaattgcggc
     1441 aaccttctcg cgtaaacgtc tgtttggtta tacctcgctg gtatgggcaa ccgtctgcat
     1501 caccgtgctg tcgttcatcg tttggctgca ccacttcttt acgatgggtg cgggcgcgaa
     1561 cgtaaacgcc ttctttggta tcaccacaat gattatcgcc atcccgaccg gggtgaagat
     1621 cttcaactgg ctgttcacca tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg
     1681 gactatcggt tttatcgtca ccttctcggt gggcggtatg actggcgtgc tgctggcagt
     1741 accgggcgcg gacttcgttc tgcataacag cctgttcctg attgcgcact tccataacgt
     1801 gatcatcggc ggcgtggtct tcggctgctt cgcagggatg acctactggt ggcctaaagc
     1861 gttcggtttc aaactgaacg aaacctgggg taaacgcgcg ttctggttct ggatcatcgg
     1921 cttcttcgtt gcctttatgc cgctgtatgc gctgggcttc atgggcatga cccgtcgttt
     1981 gagccagcag attgacccgc agttccacac catgctgatg attgcagcca gcggtgcagt
     2041 actgattgcg ctgggtattc tctgcctcgt tattcagatg tacgtttcta ttcgcgaccg
     2101 cgaccagaac cgtgacctga ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac
     2161 ctcttccccg cctccgttct ataactttgc cgtagtgccg cacgttcacg aacgtgatgc
     2221 attctgggaa atgaaagaga aaggcgaagc gtataaaaag cctgaccact atgaagaaat
     2281 tcatatgccg aaaaacagcg gtgcaggtat cgtcattgca gctttctcca ccatcttcgg
     2341 tttcgccatg atctggcata tctggtggct ggcgattgtt ggcttcgcag gcatgatcat
     2401 cacctggatc gtgaaaagct tcgacgagga cgtggattac tacgtgccgg tggcagaaat
     2461 cgaaaaactg gaaaaccagc atttcgatga gattactaag gcagggctga aaaatggcaa
     2521 ctgatacttt gacgcacgcg actgcccacg cgcacgaaca cgggcaccac gatgcaggcg
     2581 gaaccaaaat cttcggattt tggatctacc tgatgagcga ctgcattctg ttctcaatct
     2641 tgtttgctac ctatgccgtt ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca
     2701 ttttcgaact gccgttcgtt ctggttgaaa ctttcttgct gttgttcagc tccatcacct
     2761 acggcatggc ggctatcgcc atgtacaaaa acaacaaaag ccaggttatc tcctggctgg
     2821 cgttgacctg gttgtttggt gccggattta tcgggatgga aatctatgaa ttccatcacc
     2881 tgattgttaa cggcatgggg ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg
     2941 tcggcacgca cggtctgcac gtcacttctg gtcttatctg gatggcggtg ctgatggtgc
     3001 aaatcgcccg tcgcggcctg accagcacta accgtacccg catcatgtgc ctgagcctgt
     3061 tctggcactt cctggatgtg gtttggatct gtgtgttcac tgttgtttat ctgatggggg
     3121 cgatgtaatg agtcattcta ccgatcacag cggcgcgtcc catggcagcg taaaaaccta
     3181 catgacaggc tttatcctgt cgatcattct gacggtgatt ccgttctgga tggtgatgac
     3241 aggggctgcc tctccggccg taattctggg aacaatcctg gcaatggcag tggtacaggt
     3301 tctggtgcat ctggtgtgct tcctgcacat gaataccaaa tcagatgaag gctggaacat
     3361 gacggcgttt gtcttcaccg tgctaatcat cgctatcctg gttgtaggct ccatctggat
     3421 tatgtggaac ctcaactaca acatgatgat gcactaagag cggcggttat gatgtttaag
     3481 caatacctgc aagtaacgaa accaggcatc atctttggca acctgatctc ggtgattggg
     3541 ggattcctgc tggcctcaaa gggcagcatt gattatcccc tgtttatcta cacgctggtt
     3601 ggggtgtcac tggttgtgac gtcgggttgt gtgtttaaca actacatcga cagggatatc
     3661 gacagaaaga tggaaaggac gaagaatcgg gtgctggtta aaggcctgat ctctcctgct
     3721 gtctcgctgg tgtacgccac cttgctgggt attgctggct ttatgctgct gtggtttggc
     3781 gcgaatccgc tggcctgctg gctgggggtg atgggctttg tggtttatgt cggcgtctat
     3841 agcctgtaca tgaaacgcca ctcagtctac ggcacattga ttggttcgct ctccggcgct
     3901 gcgccgtcgg tgattggcta ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc
     3961 ctgctggcta tcttcagcct gtggcagatg cctcactcct atgccatcgc cattttccgc
     4021 tttaaggatt accaggcggc aaacattccg gtattgccag tggtaaaagg catttcggtg
     4081 gcgaagaatc acatcacgct gtatatcatc gcctttgccg ttgccacgct gatgctctct
     4141 cttggcggtt acgctgggta taaatatctg gtggtcgccg cggcggttag cgtctggtgg
     4201 ttaggtatgg ctctgcgcgg ttataaagtt gctgatgaca gaatctgggc gcgcaagctg
     4261 ttcggcttct ctatcatcgc catcactgcc ctctcggtga tgatgtccgt tgattttatg
     4321 gtaccggact cgcatacgct gctggctgct gtgtggtaac aaaacctctc tattaaaaag
     4381 gtgctacggc acctttttta ttgtctgaga tcagagatac ggctaataac cgtatctctt
     4441 gacagagtga gcagatgcac ataaattaat caaacggttg atagccgtat tatgtatgag
     4501 atcgactccc tacgatttca ttcagttaat caaagaagtc ataatgcctg gaaaaacagc
     4561 cgctctatac gatgtcgata aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc
     4621 cgacgccaaa aacaaacttc gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt
     4681 tattttgagc gctgctatgg aacgcgccga aagtgtgctt gataaccaac gtcgccgtga
     4741 gctttcgaat caaagctggg aactgatgaa ccaactcatc gctgaacctg ctcaaccgac
     4801 gctcgccctc aaggcgttaa tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga
     4861 ttactctgcc catagtcctc tcctgtaatt atcagtctga tattacttat cctgggcaaa
     4921 aacagtttga ttgcggtaac cctgttatcg ataaatttgt acgcgctacg cgcatcgcta
     4981 aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac ttattgacag acaaagtggt
     5041 gaactgattg gcatctgtac ttttacggca tattcgctgg aaagacaacg cgtttctggc
     5101 gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca gattagtcat gttgggggga
     5161 gcgcggaagt atcaaaagcg gggctttggt caggacctac tatgtgattt ttttgaacat
     5221 gtaaaaaaaa ttcacccggc attaccaatt aaaggggttt atcttgatgc tgaccctgcc
     5281 gccattaact tttatgctcg tctcggcttt gttcagcttt cagcgacacc aaatgctttt
     5341 ggtgctgtac ctatgttttt ggcgattcag catattctcg cggcttagat tattatcgcc
     5401 acccgccagg gcaa
//
LOCUS       DAJVRL010000175         5388 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVRL010000175 DAJVRL010000000
VERSION     DAJVRL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.176"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..945
                     /gene="tauA"
                     /locus_tag="OQN02_003778"
     CDS             <1..945
                     /gene="tauA"
                     /locus_tag="OQN02_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1644408.1"
                     /translation="NTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESG
                     ASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEAL
                     VVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQ
                     RGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAF
                     AKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAEL
                     TGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            958..1725
                     /gene="tauB"
                     /locus_tag="OQN02_003779"
     CDS             958..1725
                     /gene="tauB"
                     /locus_tag="OQN02_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1644409.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1722..2549
                     /gene="tauC"
                     /locus_tag="OQN02_003780"
     CDS             1722..2549
                     /gene="tauC"
                     /locus_tag="OQN02_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1644410.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2546..3397
                     /gene="tauD"
                     /locus_tag="OQN02_003781"
     CDS             2546..3397
                     /gene="tauD"
                     /locus_tag="OQN02_003781"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1644411.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="OQN02_003782"
     CDS             complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="OQN02_003782"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1644412.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5001..5369
                     /locus_tag="OQN02_003783"
                     /pseudo
     CDS             5001..5369
                     /locus_tag="OQN02_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1252 a         1358 c         1490 g         1288 t
ORIGIN      
        1 aacacacttc ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc
       61 gtggcgtatc aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct
      121 aaagagagcg gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg
      181 gcgctggctt ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca
      241 gccagccaac aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa
      301 gcgctggtgg taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc
      361 gtaccgttta tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt
      421 aaaccagggc tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag
      481 cggggtgata ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac
      541 ggcaaggtgc tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc
      601 tgggtggtgc gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa
      661 agcgccatcg atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg
      721 gaaaacatca gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg
      781 gtgaagggaa atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg
      841 aacaaagcaa tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta
      901 gcgaatgatt acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg
      961 ctgcaaatct ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac
     1021 ctgacgctgg aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc
     1081 accctgctga atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg
     1141 ggtaagcgta ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg
     1201 ctaccgtggc gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag
     1261 aaaatgcagc gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca
     1321 gaaaaacgct atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc
     1381 gcgctggcgg cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc
     1441 ttcacccgcg accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag
     1501 gtgctgttga ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg
     1561 ctttcatccg gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt
     1621 gttgcgggag agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa
     1681 tatgttttaa gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg
     1741 aaaaactgca ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa
     1801 gcattggcac gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga
     1861 ttagcccgct gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg
     1921 gaccgcaggg atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca
     1981 ttgtgctggc gctactcgcg gcggtggtga tcggtattcc ggtcgggatc gccatggggc
     2041 taagcccgac ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccac
     2101 cgctggctta tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct
     2161 tactgatcta tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa
     2221 gcgcgcagca ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc
     2281 tgtggtttgt cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc
     2341 tgggtgtggg ctggtctacg ctggtagcgg cggagctgat tgccgcgaca cgcggtttag
     2401 gatttatggt tcagtcagcg ggtgaatttc tcgcgactga cgtggtactg gcggggatcg
     2461 cggtgattgc gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgtc
     2521 tgacgccctg gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc
     2581 gtatatcggc gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt
     2641 tgaacagctt taccatgcgg tgctgcgcca tcaggtggta tttctgcgcg atcaagctat
     2701 tacgccgcag cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt
     2761 ttaccctcat gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc
     2821 gccggataac gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc
     2881 gattctggca gctaaagagt taccttcgac cggcggtgat acgctctgga ccagcggtat
     2941 tgcggcctat gaggcgctct ctgttccctt ccgccagctg ctgagctggc tgcgtgcgga
     3001 gcatgatttc cgtaaatcat tcccggaata caaataccgc aaaaccgagg aggaacatca
     3061 acgctggcgc gaggcggtcg cgaaaaatcc tccgttgcta catccggtgg tgcgaacgca
     3121 tccggtgagc ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgtcga
     3181 tgtcagcgag aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc
     3241 ggagtttcag gtgcgttggc gctggcaacc gaatgatatt gcgatttggg ataaccgcgt
     3301 gactcagcac tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac
     3361 gatccttggg gataaaccgt tttatcgggc ggggtgatac gagtgcggac ggttccctcg
     3421 cccccttggg gagagggtta gggtgagggg gcgttcactg tactttcaac aggttaactc
     3481 cccctttctg agaggaaaaa aattaacgca gaatcttctt ctcagccaaa tccagcgcaa
     3541 agtagctgaa aatcagatcc gcacccgcac gcttaattga acctaagctt tcgagcacga
     3601 ctttctcttc atctatagca cccgccagcg cggcgaactt aatcatcgcg tactcaccgc
     3661 tcacctgata cgcgccaatc ggcaattcag tacgttcacg cagctcacgc acgatgtcga
     3721 ggtacgctcc agcaggttta accatcaggc agtctgcgcc ctgggcttca tccagcagtg
     3781 actcacgaat cgcctcgcga cggttcattg ggttcatctg atagcttttg cggtcgcctt
     3841 ttaatgcgct tcctgcagct tcacggaacg ggccataaaa ggaagaggcg aacttggtcg
     3901 aatacgacat aatcgccgta tctttaaagc ctgcagcgtc cagcgcctga cgaatcgcct
     3961 gtacctggcc gtccatcgcg gcggaagggg cgatgaagtc tgcgcctgca gcagctgcaa
     4021 ccacggcttg cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca tgctcgcaca
     4081 gcacaccgca gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac atgacgatca
     4141 tttctggcac agtctgcttg cagatgcgcg acatacgcgc caccagtcca tcttcccgcc
     4201 aggcgtcgct gccggtttca tcggtatggt gagagatgcc aaaagtcatt acggaacgga
     4261 taccggcgtt ggcgatgcgt tcaatttcgc gtgccagatg tttctctgga atgcgcatca
     4321 cgcctggcat ggcttcaacg gctttgtagt cgtcaatttc ttcttcaaca aagatcggca
     4381 acaccaggtc gttaaggcta agtgttgtct cttcaaacat agcgcgcagc gcaggagatt
     4441 tgcgcaggcg acgagggcgt tggattaagt ctgtcatggt ctgcctgatg tttgtggaat
     4501 taaggtgcac agtatacctg aagcggggca ggagtgtttt acgaaagttg tcgcgatgtt
     4561 gacaagaaga gaatgaaaga gagaccagga catctttgta atagaaatag caataatttc
     4621 atattcactg aaaatatttt taatctttat ttatagctct tggttattat tttttggcgt
     4681 ttggttgtgc tgctataaat tgaataattc aaactttgtt gcatattgat aagatattta
     4741 tctggtttgt gtttttgata tttataacgc gtacgcatca aaaataacat tatctgcaac
     4801 atactgaaat atcgtccttt tatcaatttg atgcgtgttt ttctccataa attatatatg
     4861 tccacattcg gacttagggg aaagaataat tgaaccattc gtctgtaacg cagcataatc
     4921 gttagcgcga aacataatat gttttctatg cattgataat tgatggatca acttattacg
     4981 tccctgagga gggatgacaa atgcactcct ggaaaaagaa acttgtagta tcacaattag
     5041 cattggcttg cactctggct atcacctctc aggctaatgc agcgaccaac gatatttctg
     5101 gtcaaactta caatactttc catcactaca acgacgccac ctatgctgat gacgtttact
     5161 atgatggtta tgtaggctgg aacaactatg ccgctgatag ctattacaac ggcgatatct
     5221 acccggtcat taataacgct accgttaacg gcgtgatttc tacctactat ctggacgacg
     5281 gtatttctac caataccaac gccaatagtc tgacaatcaa aaacagcact attcacggta
     5341 tgatttattc cgagtgcatg acaactgatt agactggccc cctgaatc
//
LOCUS       DAJVRL010000176         5382 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVRL010000176 DAJVRL010000000
VERSION     DAJVRL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.177"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..783)
                     /locus_tag="OQN02_003784"
     CDS             complement(<1..783)
                     /locus_tag="OQN02_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1644413.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDL"
     gene            complement(798..1535)
                     /locus_tag="OQN02_003785"
     CDS             complement(798..1535)
                     /locus_tag="OQN02_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS1644414.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(1423..2889)
                     /locus_tag="OQN02_003786"
     CDS             complement(1423..2889)
                     /locus_tag="OQN02_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCS1644415.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(2889..4511)
                     /locus_tag="OQN02_003787"
     CDS             complement(2889..4511)
                     /locus_tag="OQN02_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HCS1644416.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(4514..5086)
                     /locus_tag="OQN02_003788"
     CDS             complement(4514..5086)
                     /locus_tag="OQN02_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCS1644417.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(5070..>5382)
                     /locus_tag="OQN02_003789"
     CDS             complement(5070..>5382)
                     /locus_tag="OQN02_003789"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1644418.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT         1129 a         1540 c         1365 g         1348 t
ORIGIN      
        1 gaggtcatca tcactgtcgc cggttggacc tccgcccatc tctggtactt tgtccggtag
       61 cggttgctgc ggggacaggt tgatgttgag attaacgccc tgcggcaaat ccccctcatc
      121 tcctgtaacc gatgcgggag ccgactccac cagttcgttc atggtgtcag cgtcacccgt
      181 tttgatggcc gtgcgcatgc gggtccacca gcttttcttt tgatttgcca ttgtgtctct
      241 gtctccaatt gcacaacgat ttccggctct gcctttgggg acaagagcca catggtttcc
      301 ggtaatatcg acctgctcgg ctttacctgg ctcggtctgc tcgtactccg cgtcatagcc
      361 gcacgacact tcacgcaggc catcttcgat aagctgaatg gcgttttcgt ctttgacgat
      421 aaggtcagcc agcatcaaat cagagtggtc acccgtcccg cgccgcacat tctgaagatg
      481 cccgacagca agctctttcc agttctcggg atttaccagc cgcacattcc cgttttcatc
      541 ttcaggatgc aggatcgtga tgctcatccc ttcgaatgag gcgagcgtgg ccggatggaa
      601 tacctgctca ggagagcgcg ttacgactat ctcaccgagc ttgtcgggtt tgaggtttgg
      661 cagatcggca gcgccgtaga gctgtttacc cgttcgacct atcggcacgt ctttgcacag
      721 cagcgagccg tcagccagct gatagcgggt ttcccccagc cgggtattga aaaaatattt
      781 catggtttac ctgcgattca ggcgagataa gaatgagggt tgggaaaaac gatttcttta
      841 taacagcgac aattcgggag ctcgccagcg tgaccggtca tgccatcaag cgttggaggt
      901 ttgccccatt cgacaaactt accttccatc tcccgatgag aatgccggac gtcgccatct
      961 tcggctgtac gccagatata accattcgag ccgattgaca gcgcacgcgc ctgatcgagc
     1021 gcgccggttg cacgtccaag ctcggtacgg gcgataaggt tcgctcgtga gcgtgacacg
     1081 tcaccggacg ctgctatctc tttcgcgaat ggttcagcgc ggccaccagc cacaacggcc
     1141 tcgatggcct tgttctgaat gtcatacacc cgatcggcgg cctcaagagg cagtgacttg
     1201 atatacttaa tttgctcggc gacgatggat ttcatcacct ggcctaccgg ggcgcggtcg
     1261 accatgttgc gcagctctgc gctgatgttc cggctgtgct gacgccactg cttttcattc
     1321 tggcgtgcaa tgtcggcggt aaagttctca gcaaccttcg tcgcccaggg ggtgatgatt
     1381 tcgctgtagc gctccagcgc atccattatt tcggtaacgc tatcatttga accatcgtag
     1441 cgcccattta cgatatcccc gaccgcccgc gctatctgcc gtaggctcgt tcgatatcgg
     1501 atctccgcct ggcggctctg gcggtttgtc gccaagttcg ccgatgcctg gcggcgcttc
     1561 gtcttcggca ttctcgatat cctcgtcggt aatggatgcc ccgatgccgg tgacgtcaga
     1621 gttttcgcgc aggtcggtca tcgccgcctt acgcgtcatc aatccgtcgc ccagcgcggt
     1681 actaatcgcg ttggtggtgt ttacggccac cgttgatcgg tcaacgtctg acatttgcca
     1741 tagcgggtta aactcaaacg tgaaatcgtc cggcagcggc tttccgagtt ccgagcggtg
     1801 cataatgtcc agtatccggc gcatcggcag ccgtaagcgg cgctcctgca atgagctcac
     1861 ccggtcgtaa tagttggcga ggtctgcatc accagtagag aagcctttcg gggattgacc
     1921 gaacaaccgc accagcggga taccaacggc accgctaatc tgctcagcaa actgcgaaag
     1981 aatgtcatcc agaccactaa agctgtactg gtgggtttcg aacttatccc gcgagtccat
     2041 gagcgtcata ccttcattgc tctggaactg gcggatcagg tcgatgttct tcagcaacgc
     2101 ctcgaacgcc gggcctccaa gcgcgataag ctcgcgcaac ttctccacgc tataggtacg
     2161 cagatgtgct ttgtagacca gctgcgccgc gccgacagtg gcgctgtcga acgcagtaag
     2221 ccgatcccag atacgctcta caaccgacat tccccattcg ttttcggtca tcttctgctg
     2281 gaatggcagc gtcaccccgt cgaagcgaat caggcggctg tgatggatgc gccaggccgg
     2341 gatgcccgtt gcagtggtca ccacgtcgta aaactcaggt ttgccgagat ctggccccat
     2401 ctctttaatg cggcgggtca ggaccgggtt aatcatccag cggtcgagcg ggagaatgcc
     2461 cttaaacttg ccttctccaa tggtttcgag ccgcagcggg gtcattggtg cctgcccctc
     2521 gatcatgatg aagccgaccg cgccgccgta gaggcgagac catttcagca cgtcgttcag
     2581 cgcatcccag atttgcaact catccagttg tgattcgaga atgccacgat cttttgcatc
     2641 aatttccgaa gtgatgcgaa tgcctttgcg ggtcatatca tccgggatag catcgaccgc
     2701 ttcgccgata atccaggacg aacgatagga ccattccacc agcatgcggt tacgactggt
     2761 gaaattagcc cggtaggtgg atgctgagtg ctggttaggt gtctgcatcc ccacgcgggc
     2821 aacaaagttc tcatagccat cagcggtggc ctgtgccgtt cgccgcgtgg cttgtttgtt
     2881 tcgtgccatc aggcctgtct ccctagcagc tcccagatgt tcagggctga attcattggc
     2941 gcgtagctga tcatcaccga gtcggcaagg tttggcgacc gggttccatc aggctgttta
     3001 tcaataacga tttttcccac accattaatg gaataggtcg gctgcgaaag ctcgatgatg
     3061 agtttatctt tgagtgccat gctactgctg attgagatga tttcgtccgg gttgtaatcc
     3121 ataccttcaa ccacggcgcg ccaggtattc tgaaaaagtt tacgtaaccg ccaccagctc
     3181 tgggctttgg cgttagcgaa gaagtccttg ttcaggcggg cggcctgtcc gttgtcaccg
     3241 cgcaccgctt cgtcgtccgg atcaaatacc gcgccactac ctcgaaacgg tgtggcgagt
     3301 attgacggtc ggcgcgcagc gttacgcagt tcgttgatag cgcgtgcatc gccgcgaacg
     3361 ccagcgccca acccgtcctc gtcaaagcga aactcttcga ggttgtcctg ttcgcaaaag
     3421 ccgaaaacct tctcgacgga ctgataaatg tcgctgccca caccggacca ttcccgcaca
     3481 ttctccagga ggaagccatg acgggtggaa aaggaatttt tgtccctgcc ttcgtcggcg
     3541 acgtccatcg cgcccagtcg cttgcctgtt ggctggatgc ccagcttgat atgcgcgtcg
     3601 acagcagcct gtacccattc ggatggaatc aggacgcctt ccgctgatgc actgtagttc
     3661 aggtcaagtt cctgtgccac caccaccgga ttgtcgattt tctcgcattc cctgcgatac
     3721 cactcttcat ccttgcgagg atcatcccgc cagtggaatg tgaataccgg tatcttcccg
     3781 ccatgacgct tctgagcgaa cgggttcgcc atgccgttaa ctgaactcag gtcaatacgg
     3841 caacgcgtcg tttgtgacaa cgccgcatca atcagcagag gacgctgaag gaatgcagac
     3901 tcatcaacca gataaagcgt ggtacggtca ccacggccaa tattatcgcc agcctcacct
     3961 ttgataacgg caccagtttc aggaaactca acgcgcatgt atggtgcgtg cttcttttcg
     4021 tcccacgaac ctcgaaactc tacaggtagt gtttccacga acttgcgcgc cttccagaac
     4081 aatgctttcg ggtcaccggt gctgtcgacg tattcctctt tacgggagcc gaaaccgata
     4141 accatttctt tgttgaagag acaaagcgag catgccagcc cgatcgcggt ccaactgagc
     4201 cccatttcac gggatttttc ggtaatacca ttctcccgat taccccagcg ttccataatc
     4261 cagtggatcc actcctcctg cttagggaag agtaaaaacg gaatggtcac cggcaggcca
     4321 taatcaatat tacgcgggtc cgttgtcatg ccccagtcga tgatgaactg agccggattg
     4381 gttcggtaaa actgttttag tgcaggcaat atttcaggat tctggcgaat gcgctgtagg
     4441 cgttccatcc gccattcaaa aaccatctgg taatcaggat gtttaaaatc gaaggggaat
     4501 ggtaacggca tacttagccc atcatttttc tatacgcctc tgcagcctgc tccggcgtta
     4561 agttggtaat ttctgttctg actggtcctc cgtcagcacc agtcacttca tttttgacat
     4621 tgtctttaaa cgcctgaaca gaaacatgac gcccgagcaa ctcaaggttt ttaaccttat
     4681 caggccattt gattttcttc aggagagcag cactatctgc tgatgccatc tcgacgacat
     4741 ccattcctga tagcgttgta cgccacacct taggccagtc tttaatctgc ttcagttcgc
     4801 cattggcaag cagaatgtca agcacgtcca tctgatcgat ttcagtcagc cgtcgcaata
     4861 cataggcggc gtctatacct acctcttcat tgcgttcagc ctttagctca gcaatgcgat
     4921 ctgctatgtc aggttttgac aggttctcgc taccagtggc gcgagcagtt ttctcgctgt
     4981 aacccgccct gatagctgcc tgagtggcgt ttaaatcttt taaatattcc ctgcagaaca
     5041 gttcctgctt aggtgtgagc tgtttcaaat cacacctccg aaacattgct ccaatttcta
     5101 tgattaacga tgtcagcaat catggtagtg gatacgccat atctctttgc taaagaccta
     5161 agcccattac ctacactccc gcgcacatac tcgcggcgaa tgcagatgac atcgcactct
     5221 tttaatttcg ccattccgtt cttttcacct gaacggtcag ggtatgagtt tctgcctcgc
     5281 cttgacctgt cacgattatt gtcagcgaca gtacctataa tcagatggtc aggattcacg
     5341 catgatggat tatcgcattt atgcctaaca acaaggtcct tg
//
LOCUS       DAJVRL010000177         5353 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVRL010000177 DAJVRL010000000
VERSION     DAJVRL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.178"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(75..557)
                     /locus_tag="OQN02_003790"
     CDS             complement(75..557)
                     /locus_tag="OQN02_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644419.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="OQN02_003791"
     CDS             complement(625..2574)
                     /locus_tag="OQN02_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1644420.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="OQN02_003792"
     CDS             complement(2659..2994)
                     /locus_tag="OQN02_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1644421.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="OQN02_003793"
     CDS             complement(2994..3350)
                     /locus_tag="OQN02_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1644422.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="OQN02_003794"
     CDS             complement(3350..4846)
                     /locus_tag="OQN02_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS1644423.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERPGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="OQN02_003795"
     CDS             complement(4843..5004)
                     /locus_tag="OQN02_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCS1644424.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5353)
                     /locus_tag="OQN02_003796"
     CDS             complement(5014..>5353)
                     /locus_tag="OQN02_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644425.1"
                     /translation="ERDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTL
                     LDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIE
                     HHLEMRFPQN"
BASE COUNT         1277 a         1561 c         1151 g         1364 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggg acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc tca
//
LOCUS       DAJVRL010000178         5337 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVRL010000178 DAJVRL010000000
VERSION     DAJVRL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.179"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OQN02_003797"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OQN02_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1644426.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OQN02_003798"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OQN02_003798"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1644427.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OQN02_003799"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OQN02_003799"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1644428.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OQN02_003800"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OQN02_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1644429.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1311 a         1289 c         1455 g         1282 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca acgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgttcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DAJVRL010000179         5156 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVRL010000179 DAJVRL010000000
VERSION     DAJVRL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.180"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..224
                     /locus_tag="OQN02_003801"
     CDS             <1..224
                     /locus_tag="OQN02_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1644430.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(396..725)
                     /locus_tag="OQN02_003802"
     CDS             complement(396..725)
                     /locus_tag="OQN02_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS1644431.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(730..915)
                     /locus_tag="OQN02_003803"
     CDS             complement(730..915)
                     /locus_tag="OQN02_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1644432.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(912..3551)
                     /locus_tag="OQN02_003804"
     CDS             complement(912..3551)
                     /locus_tag="OQN02_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1644433.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3759..4748
                     /gene="ldhA"
                     /locus_tag="OQN02_003805"
     CDS             3759..4748
                     /gene="ldhA"
                     /locus_tag="OQN02_003805"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1644434.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4859..>5156
                     /gene="hslJ"
                     /locus_tag="OQN02_003806"
     CDS             4859..>5156
                     /gene="hslJ"
                     /locus_tag="OQN02_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCS1644435.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLN"
BASE COUNT         1301 a         1320 c         1205 g         1330 t
ORIGIN      
        1 gtagtttgta ccgaatgttt gccgataaag gtttggtagt cgcgcaatat attcgtaacc
       61 gtcgtctcga tttttgtgca gatgcgattc gccatgccgc agatgatgaa aaactggcag
      121 gcatcggctt tcattgggga ttttctgacc agagtcattt ttcaacggta tttaagcaac
      181 gctttgggat gacgccaggc gagtatcgac gtaaattccg ctaactaatt ggcgttgcat
      241 gtactgcaac gccaattagt taaaacagat atttgactat gcatttttca gggcgaaagg
      301 caatttgaca ggagtttttg aatattcacc ttgtttcagg aaaatagatc aatcgctttc
      361 tcctgtagcg ttatcccgat attgcggttt tgctattaaa actttcgcac ccatttaccg
      421 tttatccctt gtacatattc ccccggtttg gcccgagcta cgagcttttg cccggcgagt
      481 ttcgcgatat catctacaga cacattattt tgctttgccc gttgttggta actcgcttta
      541 cgttcggcat taatttcggt tactaatttc tctgtttcag catccgtttt cagcgcaacc
      601 agataaccat aaaatgtttc acctacccgc ccctgagttc tggcttcatc cagagtcaat
      661 gccattacat tgctgcttac cagcccaaca agaaacgcac aaagaagtaa tgttttcttc
      721 atcatcgcct cagaaaagat cgctacgagt ttcaagcagc tcttcgacat ctttgtctgc
      781 cttgatgatg atctcatgct caattttaac gttcatattg atagtgatcg gttccttagg
      841 tgcagcgact tcaatgcgag gcgtacagcc aaccagcata aaagatgacg tcaacgcagc
      901 cagtaaaatt ttcattgttt ttcctcacac tcttttcctt gcggacagtc atttcccggc
      961 agacgtgcgt tctgctccaa ccatgcctgg agattatcgc caaagcgtaa actgcgccac
     1021 agcgtaaaaa tattctcttc atgatggtaa ttaaggttta ccgtaccgct tttaccatca
     1081 acgcgactgg tacctgtaat gttggcctgc atggttaata aaccgagatt atctaagtta
     1141 atttttgtcg atgaccggct aatttccata tagcgcaacc agttaattgc tgaacccgca
     1201 gtcatattgt ctttcaccac cgcatctgcg gtatctttat ccagccgcaa ggtcattggc
     1261 ccgctatttg ccagccaacc atctttcact atccattttt cgttgtttag ccataacggc
     1321 aatgcaccac taaatgcccc ggacatggcg aattgtttcg gattgacggc gctaaccagt
     1381 tcgctggatg agagattatt cagccgcaac agcgccgggt catgttgcgg catacgtaat
     1441 tgtttcatca gcacgttacc gcctaacaca tcgacgctaa catcagtcaa cagcaagggt
     1501 tcttcttcag tccacggata acgcccttgc aaatcagccg taatattttt cgctgtcacc
     1561 agattaatca cttcggcaat tcgcaacgta acggggccgc gagtccccag atgccaggct
     1621 ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt taacctgatt atctggcatc
     1681 caggcactac cgcctttcaa cacgccgtgc cctcccgcgc ggaatccttg ttcaggcgca
     1741 gcagaaaatg caacctgagc atacagttca ccatcgcgta agttcatctt ccagtcgggt
     1801 ggcaccagcg gctggaatac agtcagtgat tgttttggcc accaggcgtt accgcgcaga
     1861 cgaataccgt cccagcgacc atttacccga accgggccaa tctcaccagc atgtaaatcg
     1921 cctttaaaga ggaaataggt cggattgcgc ccatcgacgc taaattttaa ggttgatggc
     1981 ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt ccagtgacag cgcgccgctg
     2041 aaggagggat gctgtgcgtc acgtacccag acgacgggct tgtcgagaat taatcgcggc
     2101 ttttctaccg tcatcgtacc gtattgtaac tggtcgaaac cggtggagag atcggtcagc
     2161 gtaatcgtgc tgtcatgcca ctcacctttt cctgcgacat cccaggtggc attcatcggt
     2221 gtaaaactcc cttttcccca gtagcgccac tgccagcggc cagcgtcagg gagaaaatca
     2281 ttcgccagcc catccatatg cagcacgaaa tcgcccagtt cattttcatg cgcctgcaaa
     2341 atggcctgca aacgtccgtc aacaccacgt tgggtgactt ttacacccgc taaaggccag
     2401 cggatttcat cgatgtccag cgaatcgatg actcttccct ttgaacgaag taacgcgcct
     2461 ggctcaaagg tcagcgttgg gtcagacaga cttccactta gctgcgcagg taaacgggca
     2521 tataaaatga ggtccgcctg tttcgcttca ccggtcagct gcagaggcag ttgactgtta
     2581 tccatgctta attttcctgg gccaaaatta agcaccgcgt tgcccttacc cgcttgcccc
     2641 tgggtcagca cattcagtcg tccgctgacc agagcgttct caagccctgc ctgccagttg
     2701 tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacagcca gctccagcga
     2761 ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag caacggatcg
     2821 ccgttatccc gtgccagcac gatcaattgc ccgctatttt cctgccagtc cagctcggca
     2881 tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgccc acttacagga
     2941 agtccatccg gcacgagcgg catagtaaat tcccccacca gtttaaccgg cggctgattt
     3001 tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag ctggccttga
     3061 aatttaactt tttcgccctg ataacgcagt tgctggatat cagaggttaa tgcgagagag
     3121 agttttccct gccattcctg ccagggagaa aaaatcagtt tatcgatatt gatccaggtg
     3181 ttaggcagca tggactgcca ctgcgcgagg gtttttggag cagctggcga ttgctccgtc
     3241 tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgtt caataaccag
     3301 cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc tcccaccaga
     3361 taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc atctaatgca
     3421 atacgtgtcc cgagcggtag ccagatgtcc gccagcgtgg gaacccacag cccgagcgtc
     3481 atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac ggctttatat
     3541 ttacccagca tgggtagtta atatcctgat ttagcgaaaa attaagcatt caatacgggt
     3601 attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat agttgttgat
     3661 gaatttttca atatcgccat ggctttcaat tatatttgaa attttgtaaa atatttttag
     3721 tagcttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc gtttatagca
     3781 caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc tttgagctgg
     3841 aattttttga ctttctgctg acggaaaaaa ccgctaaaac tgccaatggc tgcgaagcgg
     3901 tatgtatttt cgtaaacgat gacggcagcc acccggtgct ggaagagctg aaaaagcacg
     3961 gcgttaaata tatcgccctg cgctgtgccg gtttcaataa cgtcgacctt gatgcggcaa
     4021 aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaggcc gttgctgaac
     4081 acgccatcgg tatgatgatg acgctgaacc gccgtattca ccgcgcgtat cagcgtaccc
     4141 gtgacgctaa cttctctctg gaaggtctga ccggctttac tatgtatggc aaaacggcag
     4201 gcgttatcgg taccggtaaa atcggtgtgg cgatgctgcg cattctgaaa ggttttggta
     4261 tgcgtctgct ggcgttcgat ccgtatccaa gtgcagcggc gctggaactc ggtgtggagt
     4321 atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac tgcccgctga
     4381 caccggaaaa ctaccatctg ttgaacgaag ccgccttcga tcagatgaaa aatggcgtga
     4441 tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcggcaatt gaagcgctga
     4501 aaaatcagaa aattggttcg ttgggtatgg acgtctatga gaacgaacgc gatctgttct
     4561 ttgaagataa atccaacgac gtgatccagg atgacgtatt ccgtcgcctg tctgcctgcc
     4621 acaacgtgct gtttaccggg caccaggcat tcctgacagc agaagctctg accagtattt
     4681 ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg tgaaacctgc ccgaacgaac
     4741 tggtttaatc ttgcagctcc cctgcaatcc aggggagctg attcagataa tccccaatga
     4801 cctttcattc tctattctta aaatagtcct gagtcagaaa ctgtaattga gaaccacaat
     4861 gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg gcgggatgtg taagtaatga
     4921 caaaattgct gttacgccag aacagctaca gcatcatcgc tttgtgctgg aaagcgtaaa
     4981 cggtaagccc gtgaccagcg ataaaaatcc gccagaaatc agctttggtg aaaaaatgat
     5041 gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta atggtgaact
     5101 gacagccaaa gggctggcaa tgacccgtat gatgtgcgct aacccgcagc ttaatg
//
LOCUS       DAJVRL010000180         5152 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVRL010000180 DAJVRL010000000
VERSION     DAJVRL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.181"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            188..445
                     /locus_tag="OQN02_003807"
                     /pseudo
     CDS             188..445
                     /locus_tag="OQN02_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="OQN02_003808"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="OQN02_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1644436.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="OQN02_003809"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="OQN02_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1644437.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="OQN02_003810"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="OQN02_003810"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1644438.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="OQN02_003811"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="OQN02_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="OQN02_003812"
     CDS             complement(3603..4418)
                     /locus_tag="OQN02_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1644439.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5152
                     /locus_tag="OQN02_003813"
     CDS             4515..>5152
                     /locus_tag="OQN02_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1644440.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLV"
BASE COUNT         1375 a         1173 c         1226 g         1378 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccctt gt
//
LOCUS       DAJVRL010000181         5033 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVRL010000181 DAJVRL010000000
VERSION     DAJVRL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.182"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(169..774)
                     /gene="azoR"
                     /locus_tag="OQN02_003814"
     CDS             complement(169..774)
                     /gene="azoR"
                     /locus_tag="OQN02_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1644441.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            975..4877
                     /gene="hrpA"
                     /locus_tag="OQN02_003815"
     CDS             975..4877
                     /gene="hrpA"
                     /locus_tag="OQN02_003815"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1644442.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVSETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1278 a         1247 c         1366 g         1142 t
ORIGIN      
        1 ttacggacac tgtaaaacag ttgatgaagc gattatcttt attcgagcca gacgctcgca
       61 tattgtgctt aaggaagagc acaaagcgat gttgaaatta tgggaaaaca ggtaagtgga
      121 ttgagatgtg gactgaatat ctacagtcca catcaagacc gtgtccggtt atgcagcaac
      181 aatgctgtcg atggctgctt ttgcgtcaga ctgtgctttc gctgccattt ccggaccgta
      241 tgcgatccct tcggcgaaga caaatttcac atcggtaatg ccgataaagc cgaggaacgt
      301 ggacagatac ggcgtcacca ggtccgttgg tccatctttg tggatcccac cgcggctggt
      361 aataacgatg gcttttttac ccgttaccag accttccgga ccgttctcgg tatagcggaa
      421 agtaacgcct gcgcgtgcaa ccaggtcaaa atagtttttc aactgagttg agatgttgaa
      481 gttatacatc ggtgccgcaa taacgataac gtcgtgggct ttcagctcgg caatcagctc
      541 atcggaaagc gccagagctt cttgctgacg cggagtcagc ggcgcatcgc tcggacgcag
      601 agcgccaacc agttcgccat ccagtaccgg aatcggattt gcagctaggt cgcgaacggt
      661 gatttcatca gcggagtgct tttcgctcca ttgttcaaca aaataatcgg acaactgatt
      721 agactgagag taccctgcca ggatgctgga tttaagaact aataccttgc tcatggtgtt
      781 tccttataga tgtttgaatg gacgatgccc cgttgcttgc tgacacttta ttcacaatcc
      841 tgccacagag atagcgcaat aaatcgaagt ctatgttcga atttattgaa caacgcatag
      901 aaagccgcga tgtggtactc tatatctatc atttaaaaga aaattaatca ggcagactac
      961 tgcccactaa cgttatgaca gaacaacaaa aattgacctt tactgccttg caacagcggc
     1021 tggattcgct gatgctgcgc gacagactgc gtttttctcg ccgtctgcat tgtgttaaga
     1081 aggttaaaaa tcctgatgca caacaggcca ttttccagga gatggcgaaa gagattgacc
     1141 aggcggcagg gaaagtcctg ctgcgtgaag cggcacgacc ggaaatcact tatcctgaca
     1201 atttaccggt tagtcagaaa aaacaggaca tactcgaagc gattcgtgat caccaggtgg
     1261 tgatcgtcgc cggggaaacc ggctccggta aaacgactca gctaccgaaa atctgtatgg
     1321 agctggggcg cggtattaaa gggctgatcg gtcataccca gccgcgtcgc ctggcggcac
     1381 gaacggtggc caaccgtatt gcggaagagc tgaaaacgga gccgggcggt tgcatcggtt
     1441 acaaagtgcg tttcagcgat cacgtaagtg ataacacgat ggtcaagctg atgaccgacg
     1501 ggatcctgct ggcggagatc cagcaagacc gcctgctgat gcagtacgac accatcatta
     1561 ttgacgaagc gcacgaacgc agcctgaata tcgattttct gctcggctat ttgaaagagt
     1621 tgctgccgcg gcgtcctgac ctaaaaatca ttatcacttc cgcgactatc gacccggaac
     1681 gcttttcgcg ccactttaat aatgcgccga ttattgaagt ctccggtcgg acctatccgg
     1741 tggaagtgcg ctatcgcccg attgttgaag aagccgatga caccgagcgc gatcagttgc
     1801 aggcgatttt tgacgccgta gacgaactaa gccaggaaag ccccggcgac attctgatct
     1861 ttatgagcgg cgagcgggaa attcgcgata ccgccgatgc gctgaacaag ctgaacttac
     1921 gccataccga aatcttgccg ctttatgcgc ggctttcgaa cagcgaacag aaccgcgtgt
     1981 tccagtcgca cagcggacgg cgcattgtgc tggcgaccaa cgtctcggaa acctcgctga
     2041 ccgtaccggg gattaaatac gtcatcgacc ccggtacagc gcgtatcagc cgctacagct
     2101 atcgcaccaa agtgcagcgt ttgccgattg aacccatttc gcaggcgtct gccaatcagc
     2161 gtaaaggccg ctgtggtcgt gtgtccgaag ggatctgtat tcgtctctat tccgaagacg
     2221 atttcctctc gcgcccggag tttaccgatc cggagattct gcgtaccaac ctggcctcgg
     2281 ttattttgca gatgaccgcg ctggggctgg gcgatatcgc tgcgttcccg tttgtcgaag
     2341 caccggataa acgcaatatc caggatggcg tgcgtctgct cgaagaactg ggcgcgatca
     2401 ccacggatga acaggccagc gcctataaac tgacgccgct cggtcgccag ctctcgcagt
     2461 tgcctgtcga cccacgtctg gcgcgtatgg tgctggaagc gcaaaaacat ggctgcgtgc
     2521 gtgaggcgat gattatcacg tccgcgctct ccatccagga tccgcgcgaa cgtccgatgg
     2581 acaaacagca ggcatcggac gaaaaacatc gtcgcttcca cgacaaagaa tccgacttcc
     2641 tcgcgtttgt aaatctgtgg aattatcttg gcgagcagca aaaggcgctt tcttccaacg
     2701 ccttccgtcg cctgtgtcgt accgattacc tcaactatct gcgcgtgcgc gaatggcagg
     2761 atatctacac ccagttgcgc caggtggtga aagaacttgg cattccggtc aacagcgaac
     2821 cggcggagta tcgtgaaatt cacatcgcct tactgaccgg tttgctttcc catatcggca
     2881 tgaaagatgc cgataaacag gaatataccg gtgcacgtaa cgcgcgtttc tccatcttcc
     2941 ccggttctgg tttattcaaa aagccgccga aatgggtaat ggtggcggaa ctggtagaaa
     3001 ccagccgcct gtgggggcgc attgctgcgc gtatcgaccc ggaatgggta gaacccgttg
     3061 ctcagcattt gattaaacgc acctacagcg aaccgcactg ggaacgggcg cagggcgcgg
     3121 tgatggcaac ggaaaaagtc actgtttatg gtttgccgat tgttgctgcg cgcaaggtca
     3181 actacagcca gatcgattcg gcgttatgtc gtgaactctt tattcgccac gctctggtgg
     3241 aaggtgactg gcagacgcgt cacgcattct tccgtgaaaa cctgaaactg cgggccgaag
     3301 tggaagagct ggaacacaaa tcacgtcgcc gcgatattct ggttgatgac gaaacgctgt
     3361 ttgagttcta cgaccagcgc atcagccacg atgtaatctc cgctcgtcac ttcgatagct
     3421 ggtggaaaaa agtcagccgc gaaacgcctg atttgctcaa ctttgaaaag agcatgttga
     3481 tcaaagaagg ggcggaaaaa atcagcaagc tggattaccc gaacttctgg catcagggca
     3541 atctcaagct gcgtttgagc tatcagtttg agccaggcgc ggacgcggac ggtgtgaccg
     3601 tacatattcc gctgccgtta cttaaccagg ttgaggaaag cgggtttgaa tggcagatcc
     3661 ccggtctgcg ccgcgaattg gtgattgctc tgattaaatc gttgccgaaa ccggtacgcc
     3721 gtaattttgt gcctgcacca aactacgccg aagcgttttt aggccgcgtc acaccgctgg
     3781 agttaccgtt gctcgacagc cttgagcgcg agttacggcg gatgaccggc gttaccgttg
     3841 accgcgaaga ctggcactgg gatcaggtgc ccgatcacct gaaaatcacc ttccgcgtgg
     3901 tggatgacaa aaacaagaag ctaaaagaag ggcgctcgct acaagatctg aaagatgcgc
     3961 tgaaaggcaa agtgcaggaa acgctgtccg cggtggcgga tgacggtatc gagcagagcg
     4021 gcttacatat ctggagtttt ggtcagctgc cggaaagtta cgaacagaag cgtggcaact
     4081 ataaagtgaa ggcgtggccg gcgctggtgg atgaacgcga cagcgtgacg atcaaactgt
     4141 ttgataaccc gctggagcaa aaacaggcaa tgtggaatgg tcttcgccgt ctactgctgc
     4201 taaatatttc ctcgccgatc aagtatttac atgagaagtt accaaacaaa gccaagctgg
     4261 ggctgtactt taacccgtat ggcaaagtgc tggagctgat cgacgactgt atctcctgcg
     4321 gtgtggatca actgatcgac gccaatggtg gcccggtctg gacggaagaa ggctttgctg
     4381 cgctgcatga aaaagtgcgc gccgaactga atgacacggt ggtggatatt gcgaagcagg
     4441 tcgagcaaat cctcactgcg gtgttcaata tcaacaaacg tctgaaaggg cgggtggata
     4501 tgaccatggc actggggctt tctgatatta aagcgcagat gggcgggttg gtatatcgcg
     4561 gttttgtcac tggtaacggc ttcaaacggc tgggcgacac gctgcgatat ttgcaggcga
     4621 ttgaaaaacg actggaaaaa ctggcggttg atccacatcg cgatcgtgca cagatgctga
     4681 aagtcgaaaa cgtccagcag gcgtggcagc aatggatcaa caaactgccg cctgcacgtc
     4741 gtgaggatga agacgtgaaa gagatccgtt ggatgataga agagctgcgc gttagttact
     4801 tcgctcaaca acttggtacg ccttatccga tttcagataa gcgtattttg caggcgatgg
     4861 agcagattag cggttaaccc tgctatttgc ctaataaatc taaaacccgg taagcattta
     4921 gcgccgggtt ttttattaat tctaaaacgg taaggacaaa aattcaggaa ttcagaaaaa
     4981 tacaattctc agctgcaaga tgaataatgt ttatctacag catttcctta aaa
//
LOCUS       DAJVRL010000182         4973 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVRL010000182 DAJVRL010000000
VERSION     DAJVRL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.183"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..369
                     /locus_tag="OQN02_003816"
     CDS             <1..369
                     /locus_tag="OQN02_003816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644443.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OQN02_003817"
     CDS             complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OQN02_003817"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1644444.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OQN02_003818"
     CDS             complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OQN02_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1644445.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OQN02_003819"
     CDS             complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OQN02_003819"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCS1644446.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OQN02_003820"
     CDS             complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OQN02_003820"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1644447.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3447..>4973)
                     /gene="tap"
                     /locus_tag="OQN02_003821"
     CDS             complement(3447..>4973)
                     /gene="tap"
                     /locus_tag="OQN02_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCS1644448.1"
                     /translation="NGMSFWAFRDDLQRLNHGEQSNQQRAALAQTRAVMLQASTALNK
                     AGTLTALSYPADDIKTLMTTARASLTQSTTLFKSFMAMTAGNEHVRALQKETEKSFAR
                     WHNDLEHQATWLESNQLSDFLTAPVQGSQNAFDVNFEAWQLEINHVLEAASAQSQRNY
                     QISALVFISMIIVAAIYISSALWWTRKMIVQPLAIIGSHFDSIAAGNLARPIAVYGRN
                     EITAIFASLKTMQQALRGTVSDVRKGSHEMHIGIAEIVAGNNDLSSRTEQQAASLAQT
                     AASMEQLTATVGQNADNARQASELAKNAATTAQAGGVQVSTMTHTMQEIATSSQKIGD
                     IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKGLI
                     EESVNRVQQGSKLVNNAAATMTDIVSSVTRVNDIMGEIASASEEQRRGIEQVAQAVSQ
                     MDQVTQQNSSLVEEAAVATEQLANQADHLSSRVAVFTLEEHEVARHESAQLQIAPVVS
                     "
BASE COUNT         1135 a         1454 c         1160 g         1224 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac
      361 tacaaataac catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg
      421 gcatcatcgc gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa
      481 atcgtccacc tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc
      541 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
      601 cagcaccatc agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac
      661 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
      721 aaagctggta tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg
      781 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
      841 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
      901 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
      961 ggcttccgcc gcttcggcaa tggcctgatc cagccccagt tcccgcaaac tgtcgcgcag
     1021 catatgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     1081 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     1141 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     1201 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     1261 atgccgacat cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg
     1321 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     1381 cgacaccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     1441 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     1501 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg
     1561 ctggcttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     1621 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     1681 acgcatcgcc aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac
     1741 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg
     1801 acggttaacc gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag
     1861 ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaggacac gttctccgtc
     1921 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     1981 gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg
     2041 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     2101 agaactcaac aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc
     2161 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat
     2221 tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc
     2281 gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg
     2341 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     2401 cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg
     2461 cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     2521 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     2581 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca
     2641 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     2701 tcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     2761 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     2821 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     2881 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg
     2941 gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg
     3001 gtgcccaatg tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc
     3061 gccgcgctcc atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg
     3121 aaatgatgag cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc
     3181 tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc
     3241 agtcccagcg aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
     3301 gccagaacga tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
     3361 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
     3421 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
     3481 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
     3541 tggtcggcct gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag
     3601 ttctgctgag tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc
     3661 cgttgttctt ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa
     3721 ctgacaatat cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca
     3781 cgattgactg actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg
     3841 gcaagattgc gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga
     3901 gccgcttcca ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata
     3961 acgctgataa tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc
     4021 atggtactga cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc
     4081 gacgcctgtc gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg
     4141 gcggccgttt gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg
     4201 cctgcgacaa tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc
     4261 accgtcccac gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca
     4321 ttacgaccat ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg
     4381 ctaccgataa tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg
     4441 atgtagatcg ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta
     4501 cgctggcttt gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca
     4561 aagttaacgt caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt
     4621 tgattacttt caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt
     4681 ttctccgtct ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa
     4741 cttttaaaca gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta
     4801 atgtcatccg ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg
     4861 gcctgtaaca ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg
     4921 ccgtgattca gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gtt
//
LOCUS       DAJVRL010000183         4930 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVRL010000183 DAJVRL010000000
VERSION     DAJVRL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.184"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(605..1021)
                     /locus_tag="OQN02_003822"
     CDS             complement(605..1021)
                     /locus_tag="OQN02_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644449.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1048..1188
                     /locus_tag="OQN02_003823"
                     /pseudo
     CDS             1048..1188
                     /locus_tag="OQN02_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1188..2228
                     /locus_tag="OQN02_003824"
     CDS             1188..2228
                     /locus_tag="OQN02_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1644450.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2256..2345)
                     /locus_tag="OQN02_003825"
     tRNA            complement(2256..2345)
                     /locus_tag="OQN02_003825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2309..2311),aa:Ser,seq:cga)
     gene            2439..3236
                     /gene="mtfA"
                     /locus_tag="OQN02_003826"
     CDS             2439..3236
                     /gene="mtfA"
                     /locus_tag="OQN02_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1644451.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3337..3412
                     /locus_tag="OQN02_003827"
     tRNA            3337..3412
                     /locus_tag="OQN02_003827"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3370..3372,aa:Asn,seq:gtt)
     gene            3574..4836
                     /locus_tag="OQN02_003828"
                     /pseudo
     CDS             3574..4836
                     /locus_tag="OQN02_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1368 a         1156 c         1106 g         1300 t
ORIGIN      
        1 tgttcagcat ggattgagcc tcagattaat gttaagcgtc ttgcaggacg cgtaatgtta
       61 actggggctt ttctctatct gccttttggt gttcatgcct gagacagata gcctcaagca
      121 cccgcagtca ttctacttaa ctaagatttc cctgcaaacc gtttttgtcc ggcacagtaa
      181 atatccaact aaaccaatag cgttcgctgt atttaccgcc agtattcaat gcacatgacc
      241 gccatgaaca cccctaaaaa aagggcattt atatgtccaa acattaatat caaaacatca
      301 attttttcca tataccttgc tgtgaagatg atgggcatac atgatgcgaa caaccagaac
      361 gcaacaaaca aaaactgcaa tgcgtttttc attattcccc ctacaatcaa tgtgcaataa
      421 catttaaaca cacctcaatt tggccggaca tataaatatc taaaccagaa aaaatcactt
      481 acatagcgtt acaaactctt tagtctaaat actcatcgta aaacattccc catacttatc
      541 agtccgttcc gcgccaggta gcttattgcc ttatctggca acctataatc aggtttccgc
      601 tttttcagtt ggctggtcgt ttaaccgaca tagttaaccc attaatctgg ttgccggatg
      661 ctggtggatt ttcgcgtttt aattgttcat aaaagtgcac agctttaacc agttcttctg
      721 atgtaaccgg gactggtggg gcagtgaata aggcctgaat ttcatagttc ggcctgtcgt
      781 tacaatcctc ttttttcggt acatatttcc agtcaccaac ccacaactcc ccctgagagt
      841 ccataacacc ttttttcacg tagcgatatc gccacgctat cggctctgct tccagcgatg
      901 cccgtgcaat tttgaataac tcgccctcta ctcgcgccat ccctgaattg gggtggcatt
      961 tcgtaatcgc tatttttaat ttggcttctt cgattaattg ttcttttgtt aattcagtca
     1021 ttttcattac cgccctggta atgctgccac cagcgaaaat ggttaatgga aaacgggctt
     1081 ccgttctata ccaatcgtag tggcaaaccg attgtcagcc gggatctatt tacctgcaat
     1141 aaaactttac caccacgcga ggtagagccg aattttggtg cgatctgatg ggaagacgaa
     1201 ggaaaaatcc tgaacacgaa aaattacctc caaatgtata cccaaataaa tatagttatg
     1261 tatggaaacc aacatccaga gaatctgtaa cactaaccgc catcaaggat ggtttagctg
     1321 ctttatggaa aaagtatgag gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc
     1381 gcttgtggga aaaattcctc gccagcgcct attacagtga cctcagtcca agaacacaaa
     1441 aagattatct gcaacatcaa aaaaagttgc ttgccgtatt cggtaaggtg ccggcagatt
     1501 ccataaaacc agaacacatc cgtcgataca tggacaagag aggggaacag agtaaaacgc
     1561 aagccaacca tgaaaaaagc agtatgtccc gcgtttacag ttgggggtat gagcgagggt
     1621 acgtgaaggc taacccatgt gcaggtgtaa gtaaattcaa ggccaaaaac cgcgaacgat
     1681 atgtaaccga caaagaatac caggcagtat taagcgttgc acctcttcct gtttttatcg
     1741 caatggaaat tgcctatctg tgtgcagcga gggtttccga tgtgttatcg ctgaaatggg
     1801 agcagattgg aaacgacggg atctttatcc agcaagggaa aacaggaaaa aaacagataa
     1861 aagcatggag tccacgatta caggcggcga tcgaaaaagc aaaacagtta ccaacatccg
     1921 cctatgtaat cagcaatcaa tacggcaacc gatatatgta caaaggcttt aacgaaatgt
     1981 gggtagaagc aagaaatcgc gcaggcaaaa tttcaggtat tttaaccgac ttcacctttc
     2041 atgatctgaa ggcgaaagga atttcagact atgaaggaag cagtcgggat aagcaacttt
     2101 tctctggtca caaaaccgag gggcaagtgc taatctatga caggaaggtt aaagtttcac
     2161 cgacacttga tgtcccgtta cctgaaaata ttccaagaaa atattccaag tgtgattttt
     2221 gtcactgact taatgatgta taagtgattg aattttggcg gagagagggg gatttgaacc
     2281 cccggtggag ttgcccccac tccggttttc gagaccggtc cgttcagccg ctccggcatc
     2341 tctccgttca gatggttgtc atgatgccag gaaatttggc attttaacag tccctgtccg
     2401 tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat gattaagtgg ccctggaaag
     2461 tacaagaatc agcacatcaa actgcccttc cctggcagga agcactatcg atcccccttt
     2521 taacgtgtct gacagaacag gaacaaagca aattggtcgc tcttgccgaa cgttttttac
     2581 agcaaaaacg gcttgttcct ttacagggct ttgagctgaa ttcattaaga agctgccgga
     2641 tagcacttct attttgcctg cccgttctgg agttaggact ggaatggctg gatggttttc
     2701 atgaagtctt aatttatcct gcgccatttg tggtcgatga tgaatgggaa gacgatatcg
     2761 gtctggtgca taaccaacgt attgttcagt caggtcagag ctggcagcaa gggcctatcg
     2821 ttttgaactg gttggatata caagattctt ttgatgcttc tggttttaac ctgattattc
     2881 atgaagtcgc tcataagctg gacacccgta acggcgatcg cgccagcgga gttcccttta
     2941 tttcgttgcg tgaggttgct ggctgggaac acgatcttca tgctgcaatg aacaacattc
     3001 aggaagaaat cgaattagtt ggtgagaatg cggcgagcat tgatgcttat gttgccagtg
     3061 atcctgctga atgttttgcc gtactttctg aatatttctt tagcgcccca gaactttttg
     3121 ctcctcgttt cccttcattg tggcaacgtt tctgtcaatt ttatcaacaa gatcctttgc
     3181 agagactgca tcacgctaat gatacagact cgttttcagc gacgaatgtt cattaattaa
     3241 caactttgaa gattaattaa ccaattgaaa tggcttatga aatttagtgt tgacagacaa
     3301 ggtaccgcta agtaatatgc gccccgttct cacgattcct ctgtagttca gtcggtagaa
     3361 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caattttctg
     3421 ttttcatgca tccttgcgaa tctttatctg atgttgattc aacaggttag tgtgaaaact
     3481 cttctcggtg cattttgatt ttaccctacg catcgggaaa aattggtggt caaatctggg
     3541 atcaggttag ttcgataatg gagtgacccc catatatccc ttaccgacgc aaaaatccgc
     3601 accctcaagc cttctgataa accctttaaa gtctccgatt ctcacggtct gtatctgctg
     3661 gtcaagccgg gtggctcccg ccactggtat ctcaaatacc gtattagcgg taaagaatcc
     3721 cgcattgcgc tgggtgccta tccagccatc tccctgtctg atgcgcgaca gcaacgtgaa
     3781 ggtatccgta aaatgctggc gctgaatatc aacccggtac agcagcgggc tgctgaacgt
     3841 ggctcacgaa caccggagaa agtttttaaa aacatggcgc tggcgtgaca taaaagtaac
     3901 aggaaatggt cgcagaacac cgccgaccgt ctgcttgcca gcctgaacaa tcacatcttt
     3961 ccggtcatcg ggaacctacc tgtatcagaa cttaaacccc gtcatttcat tgacctgctg
     4021 aaagggatcg aggaaaaagg tctgctggag gttgcgtccc gcacacggca gcacctgagt
     4081 aacataatgc gccatgcggt ccatcaggag ttaatcgata cgaaccctgc agcaaacctt
     4141 ggcggcgtga ccacacctcc tgtcagacgg cactatcctg ccctgccgct ggagcggctg
     4201 cctgaactgc ttgaacgtat tggggcatat catcagggcc gtgaactgac ccggcatgcc
     4261 gttctgctga tgctgcatgt gttcattcgc tccagtgaac tgcgtttcgc ccgctggtca
     4321 gagattgatt tcacaaaccg agtctggacg atacccgcga cgcgagaacc cattattggc
     4381 gtgcgttatt ccggccgcgg ggcaaaaatg cgaatgccgc atatcgtccc cctctcagaa
     4441 cagtccatcg ccattctgaa acagattaag gatatcaccg gtaataatga actgatcttc
     4501 cccggcgacc ataacccgta taagccaatg tgtgaaaaca cggtcaataa ggcactgcgg
     4561 gtgatgggtt acgacacgaa aaaggatatc tgcggtcacg gcttccgggc aatggcatgc
     4621 agtgcgctga tggaatcggg tttatgggca aaggacgcag tagaacgcca gatgagtcat
     4681 caggagcgca ataccgtgcg catggcttat attcataagg cagagcacct ggaagcccgc
     4741 aaagcgatga tgcagtggtg gtcggattat ctggaagcat gccgagaatc ttatgcaccg
     4801 ccttatacaa ttggtaaaaa taagtttatc ccatagtata ctaacgaatc ccacactctg
     4861 gcaaattacc accacctcta caggaggtgg ttctatatag acttttatca acttcatgac
     4921 gaaaaacacc
//
LOCUS       DAJVRL010000184         4925 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVRL010000184 DAJVRL010000000
VERSION     DAJVRL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.185"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="OQN02_003829"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="OQN02_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1644452.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="OQN02_003830"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="OQN02_003830"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1644453.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="OQN02_003831"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="OQN02_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1644454.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
BASE COUNT         1262 a         1272 c         1203 g         1188 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgct
//
LOCUS       DAJVRL010000185         4907 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVRL010000185 DAJVRL010000000
VERSION     DAJVRL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.186"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(176..760)
                     /gene="kptA"
                     /locus_tag="OQN02_003832"
     CDS             complement(176..760)
                     /gene="kptA"
                     /locus_tag="OQN02_003832"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCS1644455.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1010..1693
                     /locus_tag="OQN02_003833"
     CDS             1010..1693
                     /locus_tag="OQN02_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCS1644456.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1690..2151
                     /locus_tag="OQN02_003834"
     CDS             1690..2151
                     /locus_tag="OQN02_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCS1644457.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2164..3336
                     /gene="iadA"
                     /locus_tag="OQN02_003835"
     CDS             2164..3336
                     /gene="iadA"
                     /locus_tag="OQN02_003835"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1644458.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3401..4312
                     /gene="hypT"
                     /locus_tag="OQN02_003836"
                     /gene_synonym="qseD"
     CDS             3401..4312
                     /gene="hypT"
                     /locus_tag="OQN02_003836"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1644459.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4305..4697)
                     /gene="iraD"
                     /locus_tag="OQN02_003837"
     CDS             complement(4305..4697)
                     /gene="iraD"
                     /locus_tag="OQN02_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCS1644460.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4694..4777)
                     /gene="idlP"
                     /locus_tag="OQN02_003838"
     CDS             complement(4694..4777)
                     /gene="idlP"
                     /locus_tag="OQN02_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCS1644461.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1147 a         1291 c         1231 g         1238 t
ORIGIN      
        1 aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt aatttcagca
       61 gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa caaacgctac
      121 attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat tgaatttatt
      181 gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca accgttgatg
      241 tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc atttcctgtg
      301 ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt gccgttgctt
      361 catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt ttttttatct
      421 cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt ttttcggcaa
      481 aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac ctaccatcac
      541 tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc aatgcacggg
      601 ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc gcccatcctt
      661 cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa cttaaaaact
      721 tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg tccctggatt
      781 ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg caactcaatc
      841 ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac gcatagccag
      901 aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag ttccaggaat
      961 tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta tgacacaaca
     1021 aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag ggtatctggc
     1081 tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca gctggtggcg
     1141 cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg cgaatggagc
     1201 caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc tctttgcgct
     1261 ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg atggtcttgg
     1321 tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt tgggcattcc
     1381 agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg ccgccggtat
     1441 gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga ttttcgccgc
     1501 atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg tcgcggtgtt
     1561 tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt ttaaatttgt
     1621 cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc caacgcaagg
     1681 agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat cgatggcgcg
     1741 cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat ggcgtttgtc
     1801 atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat ctgtgaaccc
     1861 gtaatggcgc tgtggggatt atccggtgaa gcggcaaccg tccttttagc cgcattaatg
     1921 agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc attaaccggc
     1981 cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt gcaaaatgtc
     2041 ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat cattacggtc
     2101 tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta acaaggagtt
     2161 accatgattg attataccgc agccggtttt accctgctgc agggagcgca tttgtatgcg
     2221 ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat cgccgttgcc
     2281 agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag cgggcagatc
     2341 ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg cgaagcaggt
     2401 cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg cgtcacgtca
     2461 gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct gctcgccaag
     2521 acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc ttatcatgtc
     2581 ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga tcgtgtgatt
     2641 ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta tcacctggct
     2701 aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg cgtcaccgtg
     2761 ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct ggaaaactgc
     2821 gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc gttgtttgag
     2881 caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag cattgacgaa
     2941 ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc gctggcacgc
     3001 gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga agggaattta
     3061 acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt gctggtcaaa
     3121 gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt agccggtttc
     3181 cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt actggtcatg
     3241 acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt caaagacggc
     3301 aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg ccctaatgga
     3361 cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact gtggtgcggt
     3421 tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa aatgccgcaa
     3481 tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc gccgcatccg
     3541 tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc cactgcaact
     3601 ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac agttagaaag
     3661 caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca agatagccgc
     3721 tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc cgccgctctt
     3781 tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc gtgaagggca
     3841 aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt ttgaccacat
     3901 tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg gagaagcact
     3961 ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact cctacatggt
     4021 gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca ccttttttgt
     4081 ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga ttgcctggct
     4141 gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc tgaatcggga
     4201 cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca tgaatccagt
     4261 tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct gacattctca
     4321 agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag cggttcatcg
     4381 tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc ttctttcaaa
     4441 ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg cagctgacgg
     4501 caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc gggcatgcct
     4561 gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag gagatcgctg
     4621 cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa aacagcctga
     4681 agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg gcttgccctg
     4741 aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt tgttcattac
     4801 atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac cttacttgcc
     4861 tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaa
//
LOCUS       DAJVRL010000186         4867 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVRL010000186 DAJVRL010000000
VERSION     DAJVRL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.187"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..969
                     /locus_tag="OQN02_003839"
     CDS             <1..969
                     /locus_tag="OQN02_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1644462.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1550..2773
                     /locus_tag="OQN02_003840"
     CDS             1550..2773
                     /locus_tag="OQN02_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644463.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3020..3586
                     /gene="slp"
                     /locus_tag="OQN02_003841"
     CDS             3020..3586
                     /gene="slp"
                     /locus_tag="OQN02_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1644464.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3742..4272
                     /locus_tag="OQN02_003842"
     CDS             3742..4272
                     /locus_tag="OQN02_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1644465.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4314..4867)
                     /locus_tag="OQN02_003843"
                     /pseudo
     CDS             complement(4314..4867)
                     /locus_tag="OQN02_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1557 a          998 c          905 g         1407 t
ORIGIN      
        1 gcagagatgt ttgtgtttct cgccgtcgag ctttcgttgc tgtttatagt gattagtgcc
       61 ggtgtcagcc tgataagaca aaaggtgcca gaccataaaa tccagcagat gatgggggcc
      121 agaaaaggga gaggctatct cctggctgct ctgttgggag ccgttacccc gttctgtagt
      181 tgctcgacaa tacccatgtt acgtggattg ttgtcagcga aagccgggtt tggtccgact
      241 ctcacttttt tatttgtttc accattactt aatcccatta tcgtcggact aatgtggatg
      301 acctttggtt ggaaagtcac cttgttgtac gcgattatcg cagccggcgt ctccgttctt
      361 gccagtatta tcctggattc tctggggttt gaacgtcata tcattgccag taaaagctca
      421 tcagcaaatt gttgtgctcc agcaaaaact tcgccgggaa cgacatatac gcccataaaa
      481 gtgagttgtt gtagtccagc ggctaaagcc attgagaacc ccgtagttac ctgttgcagt
      541 accaaagctg tagtaagtat taatcccata aaactagcca ccaaagatgc gttgcaacag
      601 tttaaagatg tactgccata tcttttgtta ggagtattaa taggatcttt tatttatggc
      661 tttattccct cagcgtggat tgccgctcat gcaggagcag ataatccctt cgccatccca
      721 ttaagcgccg ttgtcggtat tccgctgtat atccgggctg aggcggttat ccctctggca
      781 tctgttctga tgacaaaagg aatgggtctg ggagcattaa tggcattaat catcggcagt
      841 gccggcgcaa gcctgacgga agtgatatta cttaaatcaa tgttcagaat gccgatgata
      901 gctgcattcc tgacggttat attaggtatg gctatcttga tgggctattt gactcaattg
      961 ctattttaaa tagatgccag cagtaaacta taagatgcat agttagtgcc atatccttct
     1021 aagtagaaaa tcatagacag gataatcctt aaaaatcaca aatgatttaa ctaatgcatt
     1081 gagaatgatg acggctcggt caacaagtca tcaacagaca ataaccacgt aatcatgctt
     1141 ctctgacatt ttatcggaga agcaatgact ctttgtctca gtaccctcct cctttttaaa
     1201 aggcagattt cagtcgctaa tacaacacat ttttaaatac actttaacct ggttcattat
     1261 tacacatcta aaacatcaca aaaatattac ttcacacact atcggagaac ataataactt
     1321 tttaaaatga gcgttttttg atagttgtta acgttttcct ttacgatact atcactgccc
     1381 tttttttata cacaaatatt accgggtgga aatgaaaggg aatttaaagg taattatatc
     1441 aggaagatat aacgcattat atttattgtg tgtagaagaa aatccattcc tgttctaatt
     1501 atccaattta aaaacattag cataaaacag atgatgaata aggaatgtta tgtcaattga
     1561 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
     1621 cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg aagaacaaca
     1681 acccattaat tccacattct ctggagaagg atacaaccct gaacaatttg atttagcgca
     1741 acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa atgcagacta
     1801 ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga taagttcgac
     1861 accacttacg caacagacac aatcaatact taatcagatt cataacatta gatacgaaaa
     1921 ggataaggat tctgtatgtc aaatcattgt tgtcgttaat ccagaaccca acaatccaat
     1981 cataactaaa attctcgtag aagaaggaat acccgaaaga ttcagtgtcc aaaccgtttc
     2041 ttccgataat aaaaattttg ctgggcaacg agctgactta ccaactgata taagagacat
     2101 tcaaagcttg tacttaaaaa tggcgaagct gtatattgag cacagtaaaa atgaaaatag
     2161 gatggaagcc cttgctggat gcgactttat tgatttcaac atgacagggc aagatatgtc
     2221 caagctagtc ctaaccttat caaaatttta ttttgaagat ctacttaata taaatttcac
     2281 tgacgcaaat ttatttaaca ctattttttc acacaaggaa aatcccatcc ccaaactaca
     2341 taaatatgaa caacatcttg acaaacaaat caatggtttg ttttctacat tgttaactat
     2401 taatgacaac tctttacgcg cgaaagcgga aattgcttcg agaataattg atttcttaga
     2461 ggcaaaggta gtgaatcttt catttgatga catattaaaa tataagcaag agttcaaaaa
     2521 aatatgttat aaacaacttc aagagtttac aacaccctct ctatacaata aaattcaaaa
     2581 atgggcaaca atgtctaaaa atgaattcat agcatttcat tacgagacac ttcaacccga
     2641 aaagatttca tacccctttt atttaaagag agatctgcct aatgaaaaag atataaatta
     2701 tggggtggaa atagaaatac catctggcaa aagaattcgc ttatcaaatc attatcagaa
     2761 tataatacca taatattgat tattagcact ttataatcat tgaataaaaa tagattttat
     2821 gtaattttaa aacaaagggt tatattattg ggcgatggat actcatacca cgccccaaaa
     2881 taacggaatt gaactaaaca gaagggatat aaacatcaga caggtttacg ttactatcag
     2941 gcatatcacc tcagaatcag atgaaaacta taaagaaata tctattatgg ttttaatatt
     3001 tgttgataag gatagtaaca tgaacatgac aaaaggtgca ctcatcctca gcctttcatt
     3061 tttgcttgcc gcatgtagtt caattccgca aaatatcaaa ggcaataacc aacctgatat
     3121 tcaaaaaagt tttgttgctg ttcataacca gccggggtta tatgttggtc aacaagcgcg
     3181 ctttggtggg aaggttatca acgttatcaa tggcaaaacg gatacgttgt tagaaatcgc
     3241 tgtattaccg ttggatagct atgcgaagcc tgatattgaa gccaactatc agggccgact
     3301 gctcgccaga caaagcggct tccttgatcc agtgaactat cgtaatcact ttgttaccat
     3361 cctcggtacc attcagggtg aacaaccagg ctttatcaat aaagtcccgt ataacttcct
     3421 ggaagtgaat atgcagggca tccaggtgtg gcatttgaga gaagtggtta ataccaccta
     3481 taacctgtgg gattacggct atggtgcatt ctggccggaa ccgggctggg gtgcgcctta
     3541 ctacaccaat gcggtgagtc aggtaacacc tgagctggtc aaataacacc accgaaagat
     3601 gcagatgctt tctcagcatc tgcatcatgt attacataaa attaatatac agtaagctaa
     3661 ctattattat tataagccct gtcctgttaa ttacctttgg caaactgatt ataaagttaa
     3721 tgtccgcacc aggagtcggt tatgtttctt ataattacca gggatacgat gttcttcacc
     3781 gcgatgaaaa acattctgag taaaggtaat gtcgttcata tacagaacga agaagagatc
     3841 gacgtaatgt tgcatcagaa tgccttcgtc attattgata cattaatgaa taatgtattt
     3901 cattctaatt ttctcactca aattgaacga ttaaaacctg tccatgtcat tgttttctcc
     3961 ccctttaata ttaaacgctg tctggggaaa gtaccggtga cctttgttcc gcggactatc
     4021 actatcattg attttgtcgc actcatcaat ggcagttact gctctgtgcc tgaagcgaat
     4081 gtgtcacttt cgcgcaagca acatcaggtt ctgagctgca ttgcgaatca aatgacaact
     4141 gaagatattc tggagaaact gaaaatatcg ctaaaaacgt tctactgcca taaacacaat
     4201 atcatgatga tcctcaatct taagcggatc aatgagctgg tacgccatca gcatattgat
     4261 tatctggtgt gaatttcagg cttacggtga gtctggctac gctgccacac agattagcta
     4321 attgaaacgc ctttcacccc tgccatacct tttaataatc gcaacaggtc ttctatcaac
     4381 gttgtggcat gaagctggat atcaatggcg accacttcat gatcttcatt ttcctgcaac
     4441 gaaactaaat cagtttttat tttttgctgc ttaaaccagt cgagcattga taccgtatta
     4501 ccgttcacta atgtcagctg taaatggtaa tttttgttca tcaggcggaa ggttaattga
     4561 tggaagactt ccagcaccaa caaggtcatc actgaaccat aaatacccag ttcgtacata
     4621 ccgctgccaa taaccatacc tatgcggcgg tcacccagat atccgctgcc gtcgtcagac
     4681 ctacaatatt ttggttacga acgagaatgt tacctgcacc gataaacccg acgcccgtca
     4741 ccacctgagc agcaatacgg ctggggtcga gtccgacgtg atctaaagac agcacgtcag
     4801 caaaaccata tttcgaaaca atcataaaca gggcgcttcc catgccaatt aatacatgtg
     4861 tgcgtaa
//
LOCUS       DAJVRL010000187         4860 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVRL010000187 DAJVRL010000000
VERSION     DAJVRL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.188"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..425
                     /locus_tag="OQN02_003844"
     CDS             <1..425
                     /locus_tag="OQN02_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1644466.1"
                     /translation="PRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLG
                     VYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG
                     TVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            759..3038
                     /gene="ydeP"
                     /locus_tag="OQN02_003845"
     CDS             759..3038
                     /gene="ydeP"
                     /locus_tag="OQN02_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1644467.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3286..3489
                     /gene="safA"
                     /locus_tag="OQN02_003846"
     CDS             3286..3489
                     /gene="safA"
                     /locus_tag="OQN02_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1644468.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3557..4318
                     /gene="ydeO"
                     /locus_tag="OQN02_003847"
     CDS             3557..4318
                     /gene="ydeO"
                     /locus_tag="OQN02_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1644469.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4720..>4860
                     /locus_tag="OQN02_003848"
     CDS             4720..>4860
                     /locus_tag="OQN02_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1644470.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1388 a         1037 c         1079 g         1356 t
ORIGIN      
        1 atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg ccaacaggcg
       61 gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag
      121 aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg
      181 ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat
      241 cgggagtggg tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct
      301 ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa
      361 caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
      421 aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt
      481 attttgtgaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag
      541 tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg atgcttaact
      601 tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct
      661 tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt
      721 acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct
      781 accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc
      841 agatggatat acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg
      901 actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac atttgtgaaa
      961 acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc tctttctttg
     1021 ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc
     1081 gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc
     1141 aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg
     1201 aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc
     1261 gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg
     1321 tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga
     1381 agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga
     1441 cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg
     1501 aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg
     1561 agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca
     1621 aggggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca ggtcggccct
     1681 cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg
     1741 acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa
     1801 tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg
     1861 ggatcactca gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga
     1921 tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg
     1981 tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc
     2041 tgggtgagcg ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca
     2101 tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac
     2161 tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac
     2221 acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc
     2281 cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg
     2341 aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc
     2401 tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg
     2461 tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc
     2521 tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa
     2581 atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca
     2641 aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac
     2701 gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat
     2761 tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa
     2821 acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca
     2881 tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc
     2941 cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggc attcctggct
     3001 ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc
     3061 tgtcatccgc acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg
     3121 attaaattaa actacatact ataagatata aacttctgcc tacagctgta agaaactccg
     3181 ctcagtactg aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac
     3241 tgattaacga cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca
     3301 gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgc aattgtggtt
     3361 gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt
     3421 tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac
     3481 tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt
     3541 gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa
     3601 cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa
     3661 cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga
     3721 aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc
     3781 tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta
     3841 tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct
     3901 gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt
     3961 ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga
     4021 tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca
     4081 aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg
     4141 cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt
     4201 tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg
     4261 ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag ctatttgatt
     4321 atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg cagccattcc
     4381 ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac atccatcaca
     4441 tatttatgca cttgcataac ctgttgcatg attatttatg atctcaattc ggcattttgt
     4501 cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg cgagaaatta
     4561 cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct ttttttagct
     4621 aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc tcttcgtaaa
     4681 attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc attaaagaaa
     4741 agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc atttgctgct
     4801 catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt ctcagacttt
//
LOCUS       DAJVRL010000188         4820 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVRL010000188 DAJVRL010000000
VERSION     DAJVRL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.189"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OQN02_003849"
     CDS             complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OQN02_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1644471.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OQN02_003850"
     CDS             complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OQN02_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1644472.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OQN02_003851"
     CDS             complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OQN02_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1644473.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OQN02_003852"
     CDS             complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OQN02_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1644474.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4185..>4820)
                     /locus_tag="OQN02_003853"
     CDS             complement(4185..>4820)
                     /locus_tag="OQN02_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1644475.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1286 a         1174 c         1140 g         1220 t
ORIGIN      
        1 tgaaggaaga gctgaatagt ctgaaggatt tgaattcggt taggaatttt atcgtggaat
       61 aacagaagct gcggcacgtc gtatgcagga acgtgccgcg gttggctggt aaactttcga
      121 tagtgcgagt attgaatgat ttctaggcgt tatcgatttt acgtattttt tgcatgatag
      181 aattcgtacc tcctcccaac gaccttccat ggctttccgg acttctgtag gctggcggac
      241 gatgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc
      301 cctttgtgcg tgcgttgatg ctggtaacgg taaacgtcat gatgaacacg accgcgaata
      361 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccagtca
      421 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
      481 ggagtactgc aatggcgcca ggcgcaatga gcagagaaat agagagaatg atacctactg
      541 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      601 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      661 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatat
      721 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
      781 taattgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccataa
      841 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      901 ctccggcgac gaatgcccct gtagccagag gtaaccccag aatccaggta agcacgatgc
      961 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
     1021 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
     1081 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
     1141 atgcgcacgc cgacgatttg ccaaaagtcc atgggtgggc gcaaatacaa atgctaaaag
     1201 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1261 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1321 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1381 cagaaacgca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1441 gaggattttt aatctgcccg gatgtaatcc aatagcacgg gcgtgattct cgtcgaaaaa
     1501 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
     1561 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg
     1621 aatgttcacg gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga
     1681 gaagatcagg ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat
     1741 gctgcccgct gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
     1801 atatgccccc gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca
     1861 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca ttgccgagac
     1921 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatcttgct
     1981 tccctctgca ccgctgacgg tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg
     2041 ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg
     2101 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
     2161 accgtataat cgcaaaatgt cgtgactgag ccgagattgt gcgttgagac aagcatcgtt
     2221 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca
     2281 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat tgctctcgcc
     2341 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgacgatg gcgaaaatcc
     2401 accatatcga cacgttccag cgcatcggtg acaatctgac gatccctctt tttggctata
     2461 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
     2521 aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc
     2581 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
     2641 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
     2701 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
     2761 gtaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccggctt
     2821 taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatac gtaggtaccg
     2881 ggccgttttc tgtgctcagg gaatcgacat agagcacacc gccgtagtgt gcgccggttt
     2941 cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga
     3001 tatgattttt cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat
     3061 cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa
     3121 aagccccttc actggtgacc atccatcgct gattctcagg gagttccgta atctgcttac
     3181 gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcatta cgttggtagg
     3241 tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca
     3301 gagcattatc tggcgacatc catgcatggg gattaggttt gccctcatag ggtccttcgg
     3361 tgatccctac tggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct
     3421 ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc
     3481 cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct
     3541 tggttatgga tgagacttct gcagcatctc cagccacgtt tttggccata tccgcgatga
     3601 tggtaaatgt tgtaatgacc ttgaattttt cagttgcact tgcctgaaat gcgatcgagc
     3661 aggtgagggc gaggcacccc aagagcatgg ttactttttt tattgagtgc ataatttagt
     3721 acctatagtg agcaaaatta gaacaaacat agcaacggct atgttttata gcataaccta
     3781 tacaaaatga aaataattat cattaacagc tttgaggcct aaaacaggga ttaggttgtt
     3841 tcctgctggc gacatgatat tgaggaaaaa agtagagcag ctattaggct ttgaaatgac
     3901 tttatgagga aaattgttga tggcaaaaat ggggcaaaac gctgtataag gggcaaaaaa
     3961 ggggcaaaaa aggggcaaaa aagagtgggt tatcgtagct tattgttgtc gctgataatg
     4021 tttaacatat tgaaaaataa ataaaatact tatgagtcag atagttgtga tttttgccct
     4081 tacttgttca ggtcgtattg ttctttcttt gtaatttgtt gattttcttg cattatttca
     4141 gttctctggt actaaatggg gcaaattggg ggcaaacttt gcaactacga taaccgcgca
     4201 ttcaacatgg ctatctgttc gtcgttcatg tcatcaatcc acataccgta aatttcatac
     4261 accatttgcg cagtttcatg ccccatctgg ctggctataa atgccgggtt cgctcctgcc
     4321 gtcaacagcc agcaggcaaa agtatgtcgc gtgtggtacg gattacggcg gcgaatacca
     4381 gcacgtttta ctgctgcatt ccatcttgta cccaaactgc ttaccgagta ataaggtttc
     4441 tgtttttcgt tacacaccct gggcatgaaa acaaaatgca gtttttgttt ttcagttctg
     4501 ccgtactccc gatgataaaa agtgatttcg cttttgcgat gatgcccggt cagtttgtat
     4561 tgctccttca gtgcttcaag agccggctgc agtaatgtta ccgtccggat cccggcattt
     4621 gtttttgggg gaccgaacat atcaagtatc gtcaggtttc ttctgacatt cacaattccc
     4681 ttctcgaaat ccacatcctc ccacgccaga gctgccagtt ccccgtgacg aagcccggag
     4741 taaacggcaa atttccacaa gttttggctc tgtccttttt cactttccat taatgcattg
     4801 aattctgttt tagataacgg
//
LOCUS       DAJVRL010000189         4636 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVRL010000189 DAJVRL010000000
VERSION     DAJVRL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.191"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..311
                     /locus_tag="OQN02_003854"
     CDS             <1..311
                     /locus_tag="OQN02_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1644476.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OQN02_003855"
     CDS             468..1217
                     /locus_tag="OQN02_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1644477.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OQN02_003856"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OQN02_003856"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCS1644478.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OQN02_003857"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OQN02_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1644479.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="OQN02_003858"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="OQN02_003858"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS1644480.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="OQN02_003859"
     CDS             4084..4557
                     /locus_tag="OQN02_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCS1644481.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1238 a         1162 c         1138 g         1098 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcatcagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAJVRL010000190         4611 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVRL010000190 DAJVRL010000000
VERSION     DAJVRL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.192"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            224..2647
                     /gene="pgaA"
                     /locus_tag="OQN02_003860"
                     /pseudo
     CDS             224..2647
                     /gene="pgaA"
                     /locus_tag="OQN02_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2656..4149
                     /gene="pgaB"
                     /locus_tag="OQN02_003861"
     CDS             2656..4149
                     /gene="pgaB"
                     /locus_tag="OQN02_003861"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCS1644482.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4153..4611
                     /gene="pgaB"
                     /locus_tag="OQN02_003862"
                     /pseudo
     CDS             4153..4611
                     /gene="pgaB"
                     /locus_tag="OQN02_003862"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1317 a         1034 c         1061 g         1199 t
ORIGIN      
        1 tttatgccgt attcctgaag atcctcatca ttggaatgga ttttcgggcg agaaaaggat
       61 tttatatgga cactctgctc atcatttctt cttctcatca tcaacaattc acgtctctct
      121 tccgcgttta ataacggatt atgaggtgca aaaatatctt tcttttcagt tacctgtaat
      181 tagatacaga gagagatttt ggcaatacat ggagtaatac aggatgtatt caagtagcag
      241 aaaaaggtgc ccgaaaacca aatgggcttt gaaacttctt actgccgcat ttttagcagc
      301 gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat gcattgatta ttgaagctcg
      361 caagggtaat actcagccag ctttgtcatg gtttgcacta aaatcagcac tcagcaataa
      421 ccaaattgct gactggttac agattgcctt atgggccggg caagataaac aggttattac
      481 cgtttacaac cgctaccgtc atcagcaatt accagcgcgt ggttatgcag ctgtcgccgt
      541 cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca ctgtggcaaa aggcgctctc
      601 tctggagccg caaaataagg attatcaacg gggacaaatt ttaaccctgg cagatgcggg
      661 tcactatgat actgcgctgg ttaaacttaa gcagcttaac tctggagcac cggacaaagc
      721 caatttactc acagaagcct atatctataa actggcgggg cgtcatcagg atgaattacg
      781 ggcgatgaca gagtcattac ctgaaaatgc atctacgcaa caatatccca cagaatacgt
      841 gcaggcatta cgtaataatc aacttgctgc cgcgattgac gatgccaatt taacgccaga
      901 tattcgcgct gatattcatg ccgaactggt cagactgtcg tttatgccta cgcgcagtga
      961 aagtgaacgt tatgccattg ccaatcgcgc cctcgcccaa tacgctgcat tagaaattct
     1021 gtggcacgat aacccagacc gcactgccca gtaccagcgt attcaggttg atcatcttgg
     1081 cgcgttatta actcgcgatc gttataaaga cgtcatttct cactatcagc gattaaaaaa
     1141 gacggggcaa attattccgc cctaggggca atattgggtt gcatcggctt atctcaaaga
     1201 tcatcagccg aaaaaagcac agtcaataat gaccgagctc ttttatcaca aggagaccat
     1261 tgccccggat ttatccgatg aagaacttgc ggatctcttt tacagccacc tagagagtga
     1321 aaattatccg ggcgcgctaa ctgtcaccca acataccatt aatacttcgc cgcctttcct
     1381 tcggttaatg ggcacgccta cgagcatccc gaatgatacc tagttacagg ggcattcgtt
     1441 tctctcaacc gtagcaaaat atagtaatga tcttcctcag gctgaaatga cagccagaga
     1501 gcttgcttat aacgcaccag gaaatcaggg actgcgcatt gattacgcga gtgtgttaca
     1561 agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa aaagcagaag tgatcgagcc
     1621 acgtaatatt aatctggagg ttgaacaagc ctagacagca ttaacgttac aagaatggca
     1681 gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa ccgcaagatc ccggcgttgt
     1741 acgtttaaaa cgtgcggttg atgtacataa tcttgcagag cttcgtatag ctggttcaac
     1801 aggaattgat gccgaaggcc cggatagtgg taaacatgat gtcgacttaa ccaccatcgt
     1861 ttattcacca ctgctgaagg ataactggcg cggttttgct ggattcggtt atgccgatgg
     1921 acaatttagc gaaggaaaag ggattgttcg cgactggctg gcgggtgttg agtggcggtc
     1981 acgtaatatc tggctcgagg cggagtacgc tgaacacttt ttcaatcatg agcataaacc
     2041 cggcgcgcgc ctgtctggct ggtatgattt taatgataac tggcgtattg gttcgcaact
     2101 ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa aatggtgtta caggcaacag
     2161 tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt aagtacggtg tctcctgggc
     2221 tttcactgat ttttccgaca gtaaccagcg tcatgaagtc tcaattgagg gtcaggaacg
     2281 catctggtct tcaccatatt tgattgtcga tttcctaccc agtctgtatt acgaacaaaa
     2341 tacagaacac gataccccat actacaaccc tataaaaacg ttcgatattg ttccggcatt
     2401 tgaggcaagt catttgttat ggcgaagcta tgaaaatagc tgggagcaaa tcttcagcgc
     2461 aggtgttggt gcctcctggc aaaaacatta tggcacggat gtcgtcaccc aactcggcta
     2521 cgggcaacgc attagctgga atgacgtgat tgatgctggc gcaacgctac gctgggaaaa
     2581 acgaccttat gacggtgaca gagaacacaa cttatacgtt gaattcgata tgacattcag
     2641 attttaagga taaatatgtt acgtaatgga aataaatatc tcctgatgct ggtgagtata
     2701 attatgctca ccgcgtgcat cagccagtca agaacatcat ttataccgcc acaggatcgc
     2761 gaatctttac tcgccgagca accgtggccg cataatggtt ttgtggcgat ttcatggcat
     2821 aacgttgaag acgaagctgc cgaccagcgt tttatgtcag tgcggacatc agcactgcgt
     2881 gaacaatttg cctggctgcg cgagaacggt tatcaaccgg tcagtattgc tcaaattcgt
     2941 gaagcacatc gaggaggaaa accgctaccg gaaaaagcag tagtgctgac ttttgatgac
     3001 ggctaccaga gtttttatac ccgcgtcttc ccaattcttc aggccttcca gtggcctgct
     3061 gtatgggccc ccgtcggcag ttgggtcgat acgccagtgg atgaacaagt aaaatttggc
     3121 gatgagatgg tcgatcgaga atattttgcc acgtggcaac aagtgcgaga agttgcgcgt
     3181 tcccggctcg ttgaggtcgc ttctcataca tggaattctc actacggtat tcaggctaat
     3241 tccaccggca gcttaatgcc tgtatatgta aatcgtgcat attttactga ccacgcacgg
     3301 tatgaaaccg cagcagaata ccgggaaaga attcgtctgg atgctgtaaa aatgacggaa
     3361 tacctgcgta caaaggctga ggtaaatcca cacgtttttg tttggcctta tggcgaagcg
     3421 aatggcatag cgatagagga attaaaaaaa ctcggttatg acatgttttt cacccttgaa
     3481 tcaggtttgg caaatgcgtc acaattggat tccattccgc gagtattaat cgccaataat
     3541 ccctcattaa aagagtttgc ccagaaaatt attaccgtac aggaaaaatc accacaacgg
     3601 ataatgcata tcgatcttga ttacgtttat gacgaaaacc gccagcaaat ggatcgcaat
     3661 attgatgtgc taattcagcg ggtgaaagat atgcaaatat caaccgtgta tttgcaggca
     3721 tttgctgatc ccgatggtga tgggctggtc aaagaggtct ggtttccaaa tcatttgcta
     3781 ccaatgaaag cagatatttt tagtcgggtt gcctggcaat tacgtacccg ctcaggtgta
     3841 aacatctatg cgtggatgcc ggtattaagc tgggatttag atcccacatt aacgcgagta
     3901 aaatacttac caacagggga gaaaaaagca caaattcatc ctgaacaata tcaccgtctc
     3961 tctcctttcg atgacagagt cagagcacaa gttggcatgt tatatgaaga tcttgccgga
     4021 catgctgctt ttgatggcat attgttccac gatgatgctt tgctttcaga ttatgaagat
     4081 gccagtgcac cggctatcac ggcttatcag caagcaggct ttagcgggag tctgagcgaa
     4141 attcgataaa acccggagca atttaaacag tgggcccgct ttaaaagtcg tgcgttaact
     4201 gacttcactt tagaacttag tgcgcgcgta aaagccattc gcggtccaca tattaaaact
     4261 gcacgaaata tttttgcact tccggtaata caacctgaaa gtgaagcctg gtttgcacag
     4321 aattatgctg atttcctaaa aagctatgac tggaccgcta ttatggctat gccttatatg
     4381 gaaggtgtcg cagaaaaatc ggctgaccaa tggttaatac aattgaccaa tcaaattaaa
     4441 aacatccctc aggctaaaga caaatctatt ttagaattac aggcacaaaa ctggcagaaa
     4501 aatggtcagc atcaggctat tccttcgcaa caactcgctc actggatgag cctattacaa
     4561 ctgaatggag tgaaaaacta tggttattat cccgacaatt ttctgcataa c
//
LOCUS       DAJVRL010000191         4571 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVRL010000191 DAJVRL010000000
VERSION     DAJVRL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.193"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(246..1148)
                     /locus_tag="OQN02_003863"
                     /pseudo
     CDS             complement(246..1148)
                     /locus_tag="OQN02_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1208..1486
                     /locus_tag="OQN02_003864"
     CDS             1208..1486
                     /locus_tag="OQN02_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644483.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1487..2014)
                     /gene="gadE"
                     /locus_tag="OQN02_003865"
     CDS             complement(1487..2014)
                     /gene="gadE"
                     /locus_tag="OQN02_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1644484.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2813..3385)
                     /gene="hdeD"
                     /locus_tag="OQN02_003866"
     CDS             complement(2813..3385)
                     /gene="hdeD"
                     /locus_tag="OQN02_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1644485.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3640..3972
                     /gene="hdeA"
                     /locus_tag="OQN02_003867"
     CDS             3640..3972
                     /gene="hdeA"
                     /locus_tag="OQN02_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1644486.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            4088..4414
                     /gene="hdeB"
                     /locus_tag="OQN02_003868"
     CDS             4088..4414
                     /gene="hdeB"
                     /locus_tag="OQN02_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1644487.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNENDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4478..>4571
                     /locus_tag="OQN02_003869"
     CDS             4478..>4571
                     /locus_tag="OQN02_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCS1644488.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1345 a          971 c          964 g         1291 t
ORIGIN      
        1 attgacctgc ctttagggtg cctttaaaat tcgtttttaa aggctacgat gacatttttg
       61 aagagtctta aaagaattaa aatacacggc gcgcctatct tgaatgtttt atcgatatcg
      121 aagcattgag catttttaac tcgttttatc ttgaatgcaa tcggcgcata acttattgaa
      181 tcccatcaaa tcccgtcttt ttttacccat tcccggttat cttgaataaa ttacctagtc
      241 ataaatacat tctggcggct accttcatcc actaatgccg tgacatacat gccaggcagc
      301 aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca cggagcccgt ggtttcatca
      361 accgtcgggt cggagaattt cagcgtgccg gtctggctgt aacgtttacc attttccaga
      421 ttgagctgta ctggcgtact gccctgaacc tgtttgattt gcccactggc gacctcttct
      481 ttcatgcgta agaaatcttg caccgactgc gtgagatcga cataaatcgg gtccagacgt
      541 tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac ggtcaccgac
      601 gatttcccgc tgacgcccgt aatcggcgag gtgacattcg cgtattgcag attgatcgtc
      661 gcctgttcaa cagccgcttt ggcgacggtg acattggctt ctgcttcatt cagctgggtg
      721 cgcgcggtgt cgtaatcctg acggctaacg tagttggtct tcagcaacga tgcctggcgg
      781 ttaaaggtga tgcgggcatt gctggcggta gagagcgctt tcgccagcga gcctttggcg
      841 gagtttagct cggcctgtaa aggtgcagga tcaatctgat acagcgaatc gccctggttc
      901 actttatcgc cttcgataaa gttgcgttta atgataatac cgcccacctg gggacgtatc
      961 tcggcaactt cataaggaac ggttctaccg ggcaattcgc tcaacacatt gaccgaacca
     1021 ggggagagtg tgacgacacc gacctcaggc gtcatggcgg cggcgttttc cgccgatttg
     1081 tcatcgcagg cggtgagcat cgcgccgcag aataacaacg gtattaacag ctttcttctt
     1141 ctgttcattt tagtccctga aaattcttga gaaataacgg ttctttaaat tgcgcgcgag
     1201 gagagatatg agggatttac agaccagcgg catagtgggg ttgtcagcca gtaaggtggg
     1261 atacaggcac agtgatcgac atggtgaggt caacgacaaa aaaaatgttg ccgttctgcc
     1321 aacagttcct gccagcattc gggcaacgaa gggttctact ggtggataca cataccaggg
     1381 gaatcaagtc gttttagcct ctcctttagt aatcaccggg ggtaatgaaa tcagcatctg
     1441 catccctcgt catgccagcc atcaatttca gttgcttatg tcctgactaa aaataagatg
     1501 tgatacccag ggtgacgatg tcgctcatac gtcttagttt cagcttgtac ataatgttga
     1561 atttctgaat ctttaatgtt tgttgcgaaa tattgagcgt cgacgtgata ttgctttcat
     1621 ttttattttt cagaatcagt tgcataattt tgaactgatt atgtgaaaaa cataattgtg
     1681 aattcttatg gggcaagtgt ttaccataag taatctcaag aagatttttg atactttctt
     1741 tgcggctaac aaaataaata ggcgctttag cttttagttt actgatgtga taaggtgcca
     1801 aaacaataat tctctcggca tctaatttct ccagaaattt aatcgcttct tcatcaagga
     1861 tatgattgtg ataggtatca atgataacct tgaagggttt attgcctact tttttgatct
     1921 ctgacaggga attgattttt atcatttcgt gattatcttt caactgccaa aagccctgta
     1981 aaagaaaaga atctttcgtc atgagaaaaa tcataacttg ctccttagcc gttatcgttt
     2041 cgaacatgtc atccttttca ttatttacat ccttgtccga atcgttgttc aatatagtaa
     2101 acgcctataa gaactttgcc cttcgtgaac taatacgtct attaacacta atcaatactt
     2161 cagatttatc catcacgcac tccatatatt gatcctaagt aagattcctg gttgttatca
     2221 gcttgtaaat agtaaacaaa aagttaacgt ttgcggatta ctgcccaaga ataagttatc
     2281 aataaccagt ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat ccgatttaaa
     2341 tatcgagtct ccttgtttcg acttaagctg gcaattggat tgccagcttt cttttaacgc
     2401 attgaaatta cctgagtaaa taacgcattc atgtcttcag gtttttatat agcgtctgga
     2461 gacacggata tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac ccggattact
     2521 tatttaaccg cccatagcat caacgcttac ggaactcttt gttcactttc ccacagcact
     2581 tttaaaacat taaacctgac tacggaaaat atcagccatg ataatgtttg aaatggctaa
     2641 tttgccatgg agtgaaaaaa attagatgaa attcagtagg ttgaaataat cactagcagg
     2701 taattatttc aatgatagtg cgcaattgat ctacaacact gcgtagcgga gagagtatta
     2761 atcggatcat agtcacatca agtgactatg atccgggtga caaccggggt aattattgct
     2821 gcttaacgaa caaactggcg aagctgaaca ggctggcggc gctaaatatc agttcaattc
     2881 ccaccagtgt ggaaaccagc gttacagaca ccatcggcgt tgcaccaagg aatatccagg
     2941 caatgacgat atccagcaca ccaataacga gctgtagcca gctgcctttc attgaacgct
     3001 gacgatacca actcatcagg cgaataaccc ctgcaacaca gaacaaaccg gcaataaatg
     3061 ccgcaatggc aaaaatgccc agctccggtg cacggatgaa gaaatagccg atcaataaat
     3121 aggcgactgc gacgaggaaa ccggataata ccggccagaa attatgactg cggttgctga
     3181 ataacccgac aataagcgca atacccgagc agattaataa tgcacccact actgtgctta
     3241 aaatatcgcc agagacgaac gggaaactga tacacagcaa cccgacgata aacagcagca
     3301 cggcaataaa ctggattgct ctgcgatgct ttttcagcat ctccagatca aacttcaaaa
     3361 ttgttgcctt atctatatat aacatagaac caccctataa aattaagaag aaaatcccct
     3421 gctatcaatc tatgccaaaa acgcgtctaa gaatgcagtc gatttaataa aaatttccta
     3481 attgcagtat ctgatgcatc tgtaactcat tgtattgaaa taaaaatatc tgattttgat
     3541 attttccatc aacatgacat atacagaaaa ccaggttata acctcagtgt cgaaattgat
     3601 tcgtgacggc tctttcactt tatagttgag gatattacga tgaaaaaagt attaggcgtt
     3661 attcttggtg gtctgcttct tctgccagtt gtgagcaatg cagcggatgc gcaaaaagca
     3721 gctgataaca aaaaaccggt caactcatgg acctgtgaag atttcctggc tgtggacgaa
     3781 tccttccagc caactgcagt tggttttgct gaagcgctga acaacaaaga taaaccagaa
     3841 gatgcggttt tagatgttca gggtattgca accgtaaccc cagctatcgt tcaggcttgt
     3901 actcaggata aacaagccaa ctttaaagat aaagttaaag gcgaatggga caaaattaag
     3961 aaagatatgt aattccggga atgcgttaca tcgtacttcc ttgcatattg aacaggccgg
     4021 aatatcttct ttaaaagcag ctattcctcc tgttcatata taatctctat attgaatggg
     4081 ttacaaaatg aatatttcat ctctccgtaa agcgtttatt tttatgggcg ctgtagcggc
     4141 tttgtcactg gtgaacgcac aatctgcgct ggcagccaat gaatctgcta aagatatgac
     4201 ctgccaggaa tttattgatc tgaatccaaa agcaatgacc ccggttgcat ggtggatgct
     4261 gcatgaagaa acagtatata aaggtggcga taccgttact ttaaatgaaa acgatctcac
     4321 tcaaattcct aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt tgtatacctt
     4381 caaaaatcaa gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa aggagtagca
     4441 agttgagcca tcttgctgct cctttttgca tttttatatg acagcagaat ttattatacg
     4501 tcttatactc gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa tgcgcggcaa
     4561 aggagcaggg t
//
LOCUS       DAJVRL010000192         4559 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVRL010000192 DAJVRL010000000
VERSION     DAJVRL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.194"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            294..854
                     /locus_tag="OQN02_003870"
     CDS             294..854
                     /locus_tag="OQN02_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCS1644489.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALMNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            797..1186
                     /locus_tag="OQN02_003871"
                     /pseudo
     CDS             797..1186
                     /locus_tag="OQN02_003871"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            1166..1723
                     /locus_tag="OQN02_003872"
                     /pseudo
     CDS             1166..1723
                     /locus_tag="OQN02_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1821..3430
                     /locus_tag="OQN02_003873"
                     /pseudo
     CDS             1821..3430
                     /locus_tag="OQN02_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3531..4022)
                     /locus_tag="OQN02_003874"
     CDS             complement(3531..4022)
                     /locus_tag="OQN02_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HCS1644490.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4027..4203)
                     /locus_tag="OQN02_003875"
     CDS             complement(4027..4203)
                     /locus_tag="OQN02_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1644491.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1400 a          728 c          901 g         1530 t
ORIGIN      
        1 aatcccaatg cctttttcaa aataaattag gattaaaata attaaatcag taaattccga
       61 tgcatgattt cggatttttc gaaagtcctg atgaaaggct gcgctacggc actcacggat
      121 ttaattgtta ttcctaatgt atctatgaat taatgtttta taaggatttt ttataaccat
      181 ttataagtat tttcaagcct ggcttgttgc aaacaatgta taaagcactt aggcaataat
      241 aattacattc agcaactatc atcatcggta ttgtttgtgg gcggaatttc atattgacaa
      301 cagtacagtt cttatatcta ttaataatag aaagagatct acaacctaca gattggtgta
      361 gctttatgga aaaagactat ttgagaatta gtagtactgt attagtgagc ttattgtttg
      421 ggcttgcttt ggttttggtg aatagttggt ttaatcagcc aggcgttgag gaagttgttc
      481 cacgatcaac ctatctgatg gtaatgattg ctttattttt tatcgatact gttgcattta
      541 tttttatgca gttgtatttc atttatgacc gtaggcaatt ttcaaactgt atacttagtt
      601 tggcttttct gagttgtttg atttactttg ttaaaaccgt cattatcatt cagcaaatta
      661 ttgaggggcg tttgacaagc agtgttgtcc aaaatgacat tgcaatctat tatttgtttc
      721 gtcagatgag tttgtgcata ttaatatttc tggcattgat gaataaagtt agtgaaaaca
      781 caaaacagcg caatttattt tccaaaaaaa tgactttgtg cataagtttg tttttgtttt
      841 tggggggccg atagttgctc acatactttc tagtcattat gagagctaca atttacacat
      901 tgccgagtta accaatgaaa atggtcaggt ggtttggaaa gcctcatatg ttaccataat
      961 gattttcatg tggttaacct tgctttcggt taatctatac tttaatggtt tacgctatga
     1021 tatctggaat ggagtgacgg tgattgcttt ttgtgccgta ctgtacaata tctctctgtt
     1081 atttatgagc aggtatagcg tgtccacttg gtatattagt cgcactattg aagtagtcag
     1141 taagttaacc gttatggtaa tatttatgtg ccatatattc agtgcgctac gagtaacaaa
     1201 gaacattgca catcgcgatc ccttaaccaa tatatttaac agaaattatt tttttaatga
     1261 actgacagtt caatcagcat cagccaaaaa aacgccttat tgtgtcatga ttatggatat
     1321 cgaccacttc aaagaagtca acgacacctg agggcatccg gttggcgatc aggtgataaa
     1381 aacagtggtg aatatcattg ggaaaagcat acgaccagat gaccttttag cgcgcgtcgg
     1441 cggcgaagag tttggcgtct tgctgacgga catcgatact gaacgtgcga aagctttagc
     1501 ggaaaggatt cgggaaaatg ttgagcgttt aactggcgat aatcctgaat acgctatacc
     1561 acaaaaagtg acgattagta ttggcgctgt tgtgactcag gaaaatgcgt taaacccgaa
     1621 tgagatctat cgactggctg ataacgcact atacgaggcg aaagagaccg ggcgtaataa
     1681 ggtggttgtg agggatgtgg tgagtttttg tgagtcacca taaagcggca ttttagcggg
     1741 tgattttggt aagcgctaaa tagctattcg cagacttgcg taacaatttt cagaaatatc
     1801 atagtactat atgatcttaa atgcttatca ttgatcaatc tgctatcacc cgcttttact
     1861 ggcaattgta ctataatttc ttaggttgca tcaccctgat gcgtggggaa acatgtatgg
     1921 tattcaacaa aaaaatgttt gtactcatta taataccagg cattctgggg gtgttactat
     1981 cgtttgctat gtcaatattt caaatgaata gagatacgac aattaccgcc ggtattctgt
     2041 taaaacagct tgataatgtt acgcaaattg tccagcatac gacaaagctt acctctattc
     2101 tggtgatgaa gccctgcaaa gacattcttg aacagttgat tgctaacgga gcattgactc
     2161 cctatgttcg aacaacgggt cttatagaaa ataatttcca aatatgttct tcagttagcg
     2221 gtttcaaaaa aatgaatgtt aacgatgttt acggtacgag ctttcacaat aaaaataaag
     2281 aatcacgtat agtatcaatt agcggtacaa gttttgtgcc aggtaaaaca gcaatagtgt
     2341 tccttatgcc aatagggaat gatatgacag cttttagtat tgtagagtca cgatatatct
     2401 atgatctaat ggatgttctt gatgatgaga atgatgattc attttctctt cgttttactg
     2461 aagggcctgc aattattagc ggtgtaaata ataatgacag gttatatatg ttaaaaagag
     2521 attttaattc tgcgatcagt caggctcgtc ttacagttac tacgccgatg atatcgttat
     2581 atccctatat cattagcaat gtgctttata ttttgccgtt atctattatt ctctctttta
     2641 ttctctattt cttatggcag cactggatgt cacggaaaat gtcgctggca gaagaaatta
     2701 aaaaaggaat gtcatccgga gaattttctg tgcattatca accagtgtgc gacacgacaa
     2761 ccaaagcgtg tctgggggtt gaagcgttaa tgcgctggca acgcgaagat ggtaaaaata
     2821 tatctccggt agtttttatc cgcgctgcag aagaggaaaa tttaataata ccgttaacta
     2881 aacatctctt tgaactgatt attcaggatg tacaaagctg gaaagtgaaa aagccgtttc
     2941 accttgggat aaatattgcc gcggaacatc ttgcacatcc tgactttgtt ggtgatgttt
     3001 tacatattaa aaatgcaata tctgataaat ttaatattgt ccttgagatt accgaacgca
     3061 atctggttga agacactgat cttgcattac agaaaataaa tgaattaagg atccatggct
     3121 gtgaatttgc agtcgatgat tttggcacgg gatattgttc ccttggcctt ctgcaaaagt
     3181 tgtctgttga ttatcttaaa atagataaaa gctttataga cacacttact acagcggaag
     3241 acgaaacgcc agttttagat attattatta agctgagtaa tcggctcaat ttgataacga
     3301 ttgcagaagg tgtcagtact cctcaccagc agaatatctt attcataata acgtgacatt
     3361 agttcaggga tacttatatg aaaaaccaat gaaagcgaca gagttttatc agtggtatgt
     3421 aaacagataa ataattaaaa atattggaat ggttttgtat tgtcttaaaa tataaacaaa
     3481 gcaatcagaa tgttattaac tgatgacaaa cgccaagatc aacaatgctg ttagtgagta
     3541 atacatatga atcgtggtgt cctgcctgta gagaatatgt agttataatc attttgacgc
     3601 gtaacataat aattaattga agtgtctgct tttatgaaga aatcagaagt gatattttca
     3661 aggatttcat cgctgatgtt atcatctttt tgtatttttt ggattttaac tgtggtgaga
     3721 taaacggtat cacggaattt cttgtatgta aaactcacat atcggcggat gaagcccatt
     3781 ttttttccat ctaccgtgta tgcgcccgtg agatacatgt ttcctgtgtt attttcagaa
     3841 atcatattta acgaaaaata ttgatccatt tttgtatgat cggtgaaatt aataacatgg
     3901 gcattacatg atatgttttg ctctgctggt tcattggaat gatgaaacgc aatatggctc
     3961 agaattgctg caataaaagc acataaaatt attcccgaaa acagaaaata tctttgagtc
     4021 atatgattac tccgctttat aatagagaga gttgcaatta tttggtgatt cagagtttaa
     4081 catttgacta caggtgaaag cataaaaccc gctcatgcta tcatttgttg caatgtaaat
     4141 gtaaggatgt attttgcagt tgacttccat tttctgcaat ttttcaataa ttggtgatag
     4201 catgtctacg gaattaaagt aattattatc atagaaataa ctgcatcctt cctgagtagg
     4261 gtgttgagca taatttgaga aagcatctat tttttttata gtgtgagatt tgctttgaaa
     4321 agccatatcg aaaaaataag aagacatata aactatcccc catgccgcaa aaaaaaacta
     4381 taagtaatgt attctttgtg gtaaatatac cttttgcaac gtaattttta tttatatttt
     4441 cagtgtgggt gccattctct tcactctcaa cactttcatt ctcattgtga ttattgtcga
     4501 gaacagttat gttgatttct tcattgattt tgaagccttt ccgtggcacc gttctgata
//
LOCUS       DAJVRL010000193         4548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVRL010000193 DAJVRL010000000
VERSION     DAJVRL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.195"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..1222
                     /locus_tag="OQN02_003876"
     CDS             <1..1222
                     /locus_tag="OQN02_003876"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1644492.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1896
                     /locus_tag="OQN02_003877"
     CDS             1222..1896
                     /locus_tag="OQN02_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1644493.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2424..3185
                     /locus_tag="OQN02_003878"
     CDS             2424..3185
                     /locus_tag="OQN02_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1644494.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3229..4308
                     /locus_tag="OQN02_003879"
     CDS             3229..4308
                     /locus_tag="OQN02_003879"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1644495.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4305..>4548
                     /locus_tag="OQN02_003880"
     CDS             4305..>4548
                     /locus_tag="OQN02_003880"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1644496.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          864 c         1119 g         1316 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgtagggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttaca aatataggtg atttttttat
     2221 tgtaggcttt tgaaggctat tataactgtg cgctaaatta atgcgttgaa taaaagaaat
     2281 aacaaaatta agaaaagcca tatttacgat atgggcaaca taaatataat ctaaaaaaga
     2341 gtaaggcgtt gtaggttggt tccgcgttca ttaccttgtt agtgaacgcg gatttatcag
     2401 ttgtcgaaat ggtttttata ataatgaaaa attttaatgt actaaccaaa gtcgtatttt
     2461 gcttgtcgtt tttatcaagc gcatttccca cctatgctgc ggatgatgtc aaagtgactc
     2521 aaaacacttc agtttttaaa ctgaagctta gtgcaacgcg attaatctat gaacaagggt
     2581 cggatggcgc agcgataact gtgacaaatc cgcaggattg gccgatgctt gtgcagagta
     2641 aagtctttga agaagataaa gtaactgcag cgccctttat tgtcactccg ccactgttca
     2701 ggcttaatcc aggccagcaa aataaaatgt gggttgtgca gactgcgcca gctttacaag
     2761 acagggaaaa attacactgg ctatgcgcca ccggcattac gcctaaaaag agtgatgttt
     2821 gggccgagaa agataaagaa aaaattgctg aaaatgtgaa cctgaacatc caggttaacg
     2881 tacatacctg cataaaactt attaccaggc catctggtat tgaaggcaag atggttaatg
     2941 cgagtgagaa gttatcgtgg aaaaagcagg gtgataaaat tgtcgcggtc aatcagtcgc
     3001 cgttctacat caacctttct tctttacagg ttggcggtag caagattgag aaactagatt
     3061 atattgcgcc gttttcgcaa catgagtttg ttatgccaaa aactagcggc caggatatta
     3121 aatggtctgt gattactgat gaaggtggtg agagtcgttt gtttatatct catttgcaat
     3181 cataatgtat tacattttta ttgaatattt ttatgccatg catattccat gttttatcga
     3241 ctgtcattaa ttttgataat ggccttactt gcgggtcagt taagtgccca ggagtggtca
     3301 tttgattctt cacagttgga aggaaacgtt agtgcggaca ctgtggcaat gtttaatcag
     3361 ggagagcaac tccccggaaa ttatcgtgtg gaaatttatc ttaacggtga aaaagttgat
     3421 gttggggagt ttccctttca tcgtccggaa tcccctgaag aaaaagagct tgttccatgt
     3481 ttaaccgttg atgaccttat ccactatggc attaaaattg ataaaagcag cagtgatact
     3541 gacaataaaa agaatcagtg ttttaaatgg aactctattg aaggtttgaa agtaaattat
     3601 gattttgata gccagcgcgt acagataacc gttccacaac tgtatctgca agataaaaaa
     3661 agtagtttag caccagtgtc gttatggaat gagggtgtag ctgcattccg catggtgtat
     3721 cagacaaata ttgatatttc caaacaaaat gataatcaat ctaccacgcg taattctcgt
     3781 tatggcaggt tcactccagg gtttaatctt ggtgcctgga ggtttcgtag ttcagtgaca
     3841 tggagcaagg aacttggcca gtcagaaaga tggcaaaggg gttacatgtg gtttgagcga
     3901 ggcataaatg cgattaaaag ccgattaacg cttggagaat cttatacgtc gtctgaggtg
     3961 tttgacagca taccttttcg cggcgggatg ttagcgaccg acgatgcgat gactccgccg
     4021 gaggacagct actacacacc tgtggtgcat ggaatagcac aaagtgaggc gcaggttatt
     4081 attaaacaaa atgggcaaat tatttttacc cgttctgtgc cgccagggcc ttttgcgctg
     4141 gataatttac caacgctggc cgttggcggg gagcttgatg ttacagtgag agagagtaat
     4201 ggcgaggagc agtatttttc cgtgccattt caaacgccgg ccatcgctct gcatgaagga
     4261 tatttcaaat atagtgtgat gggggggaat ataaagaaga aggtataaaa agctcccctg
     4321 tctccgtggg cgaaatcacg gcgatgtacg ggcttcctta tggcgtcacg gtgtatggag
     4381 ggattcaaaa cgccactcat tttaatgcaa tatctacggg tattggcatt tctctggggc
     4441 tgttagggag tttatcgaca gatattacgc gttcgattgc gaatttatat tatgggaata
     4501 aatatcgtat tcgttatagt aaatccatta gtgattttgg tactcaat
//
LOCUS       DAJVRL010000194         4522 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVRL010000194 DAJVRL010000000
VERSION     DAJVRL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.196"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(559..864)
                     /locus_tag="OQN02_003881"
     CDS             complement(559..864)
                     /locus_tag="OQN02_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644497.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGDKRGLLAVTQKQNGMFMRCDDSPGFFSWFSGVYKVN"
     gene            complement(918..1169)
                     /locus_tag="OQN02_003882"
     CDS             complement(918..1169)
                     /locus_tag="OQN02_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCS1644498.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            complement(1248..2519)
                     /locus_tag="OQN02_003883"
     CDS             complement(1248..2519)
                     /locus_tag="OQN02_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCS1644499.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            complement(2519..2944)
                     /gene="umuD"
                     /locus_tag="OQN02_003884"
     CDS             complement(2519..2944)
                     /gene="umuD"
                     /locus_tag="OQN02_003884"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1644500.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            3045..4265
                     /locus_tag="OQN02_003885"
                     /pseudo
     CDS             3045..4265
                     /locus_tag="OQN02_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
BASE COUNT         1066 a         1200 c         1196 g         1060 t
ORIGIN      
        1 ctgggaccac ggtgccacct gtatcgccgg tcaggttatc tgcctgggac cacggtccca
       61 cctgcatcgt cggcacatta ttagttaggg acaacggttc aatcaatgcc tgtcacgaac
      121 aggtattggc ttgtcgccag caacccgttg agcggagtaa tcacgcagca ggacggctgt
      181 tacaggcgat ggtgaaagct ctgttgggat ctgaacgaag catgttgagt gaaatttgca
      241 aaatttcgca gagtatgcgg cttcatcttt aaatttccgc cagcattttt aaagccagct
      301 accgacgctg cgcgttggcc ggcccttcgt ccggcgggta accagtaccg gccgatctta
      361 cgaccctgga tgaacatcat cccgtcagca gcgtctccac tgtgatgtcg tttttcacac
      421 tgtccgatca caaaatagaa cgggcaggga caacatatgg taggaaaagc agctgccaga
      481 gacacaatat attgattacc cacagtaacg ggggataacc atgtgggtaa catattgaaa
      541 gtgcaacacg ttatctgatt aattgacttt gtaaacgccg ctgaaccatg aaaaaaaacc
      601 gggggaatca tcacagcgca taaacatacc gttttgtttt tgggtgacgg caaggaggcc
      661 ccttttatca ccacatacct tgtcaaaggt ttccatttct atccttatca ggctcttgtc
      721 gttattttca ggagagatcc ggagatgttt tgtcagccag acaggatagg tatcagcgtt
      781 acgccagtac tgcaattcaa tcactgaagc cattccccag acagcgaagt ataagaaaac
      841 cacacctgcg attttgctac ccatattgtt ccttaacatt ggcttaatac attccttgct
      901 cctcgcaccg gagcacatca cagatactga ttgagatcca cttcacgcag cggctggttt
      961 tgacgttctg ttatcgtttg caccaggtct ttttcgtcca gctcatcgag catttgttca
     1021 tacaaagccg cgggcacaca ataaaaaaca gggcggttac ggtcaattat ctcgaccggg
     1081 tagccgccgc gggcactggc ggtggccacc ggatcgtttt taagttcgct gacgctggca
     1141 gtcacatcac acagaatgat atttgtcatg gttcccccct gagacaagtc acctgtatga
     1201 tcggtaaagt aagctctggc ggcaagtccc ttctgtcatt tcaacggtca gaaacgggca
     1261 accggcagct ccttccagcg tgtggtatat gccggtgaca gcatctcccg tttcatctgc
     1321 cagtccggcg caataccccg tccggcaaac cagactttcc cgagtccgga atgattaatg
     1381 ccgtcgagta ccttcatcag cgcatcgctg tgcggccgtg gctgcacgtc atcgaacaga
     1441 ttcagctgcg cgacgccgtt cggactgaaa tcattgagca ttatccccgc ttttgcgtag
     1501 cggtggccat cgagccatat cctgtccaga gacctgaccg ctgccgcgat aatgtcccgg
     1561 gtgtcacggg taggggtgtt cagtttttcc gtggccacgt taccgtaata cggctcatta
     1621 accgcaaaag gggatgtctt gatgaaggtg ctgatatgcc ggcagtactg tcgctcaccg
     1681 cgcaactttt ccgccgcgcg ctcggcatac tggcagacgg cctggcgcat ttctccgtag
     1741 gtggtgatcc gctgcccgaa gctgcgactg cagacaatct gctgtttcgg cggcggcgct
     1801 tcctccagcg aaatgcaact ctcgccgttc agctcacgta ccgtgcgctc cagcactacg
     1861 ttgaagtttt tccggataaa tgtagggttc gtcagcgaca gctgcagggc ggtggtgatc
     1921 cccataacgt gcagcttttt cgctatccgg ttccccacgc cccagatttc ttccaccggc
     1981 tgcaggctga gtaattttgc cgttctgcgt ggattttcgg gcgacagcgc cagcacccca
     2041 ctgaattgtg gccattcctt tgacgcccac tgcgccgatt tggccagtgt ttttgtggcg
     2101 ccgaagccga caccgatcgt cagtccggtt ccactgagaa catgcccacg cagctgcctg
     2161 ccaaaatcct ccagatccat acagtggcta attccccggg catccaggaa acattcgtca
     2221 attgagtatt gttcgacgcg gggcgacagc tcttccagca gggcgaccac acggttactc
     2281 aggctcgcgt agagctcgta gttgctggaa aaaacgtaca gtttttcagg aaactgcgcc
     2341 tctttgagct ggaaccaggg ggttcccatc tttataccca gccgtttggc ttcagcactc
     2401 cgggcaataa cgcagccatc gttgttactg agaacgacga caggtttgcc gcgcaaatcc
     2461 ggcctgaaaa ccttctcgca actggcgtag aaggagttga cgtctgccag ggcgaacatc
     2521 agtctgccct ccgggtttta tgaatgaagg ccgtcaccac gccgaaaatt tgcagagttt
     2581 ccggatccgg gtacagggaa gggaagtccg ggttcatcgc ctgcagggct ggccgcggcg
     2641 tcagaagcag gcgttttacc gtaaattcac cctcaatttc cgcgataaca atatccccgt
     2701 gccgcggctg ttctgcttta tccacgacca tcagatcgcc ggaatagagg ccaatatcag
     2761 tcattgagtt gccaatcgct ctgacaaaat aggtggagtg gcgccggtga atgcagtact
     2821 cattcagatc cagttcagac tcagtatagt ttgctgccgg tgacggaaaa cccgcagggc
     2881 atcgctctgt gaacaaagga atttttacta tgctggcttc tgatactgca ggaatgaact
     2941 tcatcgtttt ttccctcgct taaaactgta tatgcatggg tcttcccttg ttgtggtggc
     3001 tgaaggcatg ataatggtgt atttaatcgc cagaggtcac cgccatggac gaaaagtccc
     3061 tctacgctca tattctcaac ctgtccgatc cgtggcaggt aaagtccctt tctctcgatg
     3121 aaaatgccgg ttctgttact gtcactattg agatcgctga aaacacccgg ctagcctgtc
     3181 cgacctgcgg taaatcctgt tctgttcacg atcaccgtca tcgtaaatgg cgccatcttg
     3241 atacctgcca gttcaccact attgttgaag ccgatgttcc acgaattatg tgtccggagc
     3301 atggctgcct gacgttgcct gttccgtggg ctggccccgg aagccggtat acgttgctat
     3361 tcgaatcgtt cgttctctca tggctgaaaa tcagcaccgt tgatgctgtc aggaagcaac
     3421 ttaagctcag ttggaatgcg gttgacggca ttatgacccg ggcagttaag cgaggtcttg
     3481 cccggataaa aaagccatta tccgcccgtc atatgaatgt ggatgaggtc gcctttaaaa
     3541 aaggacatcg ttacataacg gtgatctccg atcgcgatgg tcgggcgctg gccttaacgg
     3601 atgatcgcgg cacagagagt cttgccggct atcttcgcac gctcactgat gggcagttgc
     3661 tggctatcaa aacgctctca atggacatga acgcgggcta tataagagca gcgcgtatcc
     3721 acttacccag tgcggttgag aaaatcgcct ttgaccgctt ccatgtggcg aagcaactgg
     3781 gcgaggtagt tgataaaacc cgtcagaatg aacatccgca cctccctgtt gaaagccgac
     3841 accaggcaaa aggaacccgc ttcctgtggc agtacagcga taagtggatg accgaatccc
     3901 ggcaggaaaa gctgatgtgg ctgcgtgcac agatgaagct gacgagccag tgctgggcgc
     3961 tgaaagagct ggcaaaggat atctggaaca ggccatggag cgaggaaaga cggagtgact
     4021 ggcagagatg gttggcgctg gcggctaaca gtgacgttcc catgatgaaa aatgccgcga
     4081 aaacgatagg aaaaaggctg taggggatcc tgaatgcgat gcgacacagt gtctcaaacg
     4141 gaaatgcgga ggcacttaac agcaagatca ggctgctgag gataaaagcc aggggatacc
     4201 gaaaccggga gcgctttaaa ctgggggtga tgttccacta cgaaaagctg aatatggcgt
     4261 tctgagcctt cccaccatga tcggggaaga cccatatgca tacagtataa ggggcggtga
     4321 aatttctgca agtgatgggg ggatagccgg tcagaacgtc cccgaaaaca aaccgccccc
     4381 ggtttcagga agggcgaact gaccggcaga ggcagtgtaa caccgcatgt cacaaaggca
     4441 gcactgacgt cgtcagtgct ttttttaccc gtcctcccgg acgctgaagg gcagtttcac
     4501 ccgtgcagct aaaggggcgg ta
//
LOCUS       DAJVRL010000195         4436 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVRL010000195 DAJVRL010000000
VERSION     DAJVRL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.197"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(226..429)
                     /locus_tag="OQN02_003886"
     CDS             complement(226..429)
                     /locus_tag="OQN02_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644501.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="OQN02_003887"
     CDS             complement(431..586)
                     /locus_tag="OQN02_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1644502.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="OQN02_003888"
     CDS             complement(789..1196)
                     /locus_tag="OQN02_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1644503.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="OQN02_003889"
     CDS             1273..1500
                     /locus_tag="OQN02_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1644504.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="OQN02_003890"
     CDS             1484..1906
                     /locus_tag="OQN02_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644505.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2767
                     /locus_tag="OQN02_003891"
                     /pseudo
     CDS             1984..2767
                     /locus_tag="OQN02_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2774..3520
                     /locus_tag="OQN02_003892"
                     /pseudo
     CDS             2774..3520
                     /locus_tag="OQN02_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3535..3957
                     /locus_tag="OQN02_003893"
     CDS             3535..3957
                     /locus_tag="OQN02_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1644506.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            4015..4371
                     /locus_tag="OQN02_003894"
     CDS             4015..4371
                     /locus_tag="OQN02_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644507.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT         1173 a          955 c         1205 g         1103 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagattttta tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gttggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagaggaaga tctaaatact gatctccccc taaatccccc tcgccaaaaa
     2461 cgagcgtcta aaaaattcga gccggaggct attgagctgc ccgattggtt gccggaaaca
     2521 ctctggcatg agtgggtccg gttcagacag gcattgcgaa aaccgattcg aacggagcag
     2581 ggcgatacgg gaactggaaa aattccgtca gcagggtttt acacctgagc aggtgattcg
     2641 acacagcatc gccaatgaat accagggcct gttcgcgccg aaaggtgttc ggcctgagac
     2701 gttgctccga caggttaaca ccgtctcgtt gccggacagt gcgatcccgc caggcttcag
     2761 ggggtaacgg accatgaaaa atattgcgac aggcggcgtt ctggaacgca tccgccgact
     2821 gaccccgcaa catgtaaccg ccccattcag aacggtagcg gagtggcgcg agtggcaact
     2881 tgctgaaggc cagaaacgta gcgaggagat caaccgcctg aatcgccagt tgcgggtgga
     2941 aaaaattctg aatcgctcag gcatccagcc gttgcaccgt aaatgctcgt ttgcgaatta
     3001 ccaggtgcag aacgacggcc agcgatacgc gttaagccag gcgaaatcca tcgccgatga
     3061 actgatgacc gggtgtacaa attttgcgtt cagcggaaaa cctgataccg ggtagaatca
     3121 cttagcggca gctatcggga atcgcctgct gaaagacggt cagacagtga ttgtggttac
     3181 cgtggctgat gttatgagcg ccctgcacgc cagctatgac gatgggcagt caggcgaaaa
     3241 atttttgcgg gagctgtgcg aagtggatct gctggttctt gatgaaattg gcattcagcg
     3301 cgagacgaaa aacgagcagg tggtgctgca ccagattgtt gatcgccgga cagcgtcgat
     3361 gcgcagcgtg gggatgctga caaacctgaa ctatgaggtg atgaaaacat tgctcggcga
     3421 gcgggtgatg gatcgcatgg tcatgaacgg cgggcgctgg gttaatttta actgggagag
     3481 ctggcgtccg aatgttagcc attcgagggt tgttaagtag tttcaggagg atttatggcg
     3541 aaacctttta ctcccgaaca gcgggaagaa ctgaagacgc gaattgtgga actcgtgcat
     3601 caggacggtc gggtcacgat tcggcagttg tccgatgaaa caggtatcag tcgtgcgtct
     3661 gtcggtcgct tatgcataga actggtcgca agtggtgatg tatataattc tggctacggc
     3721 ttattcccgt ctgaacaggc tcgcaaggac tggcaaagcg cccgcaaaaa actctcgaga
     3781 gtaaaggtga ggaaaccggt tgttgttgat ccggacctta tctggtcatt acctgacgga
     3841 gaaatacgcc gctacgacag gcgcctgaat ataatctgtc gcgagtgccg gaagagcgaa
     3901 gctatgcagc gtgtactggc tttctatcag ggtaattttc aggaggcgat actgtgaatg
     3961 aaattagcta tcaggcttca attaccgctg gcattcgcat caaaggagag gagcatggaa
     4021 ataaaaccag aagatgagtt aagcaatatc gttttatttc cgataaaaga ggatgaccct
     4081 cgtaatcagg ttaattttct ttataagcca tcggaaagac catattgcca tcacgcctct
     4141 gtacgggttg acgaaaaaga gcgtcaggtc cgctgtaaaa tctgcggtgc ggttgtggag
     4201 ccgtttgact ggatgctctc tgtggcaaaa agagaaacca gactggcaga tgatgtaagg
     4261 ctattgcgcc aggaggaaca ggaaaggcgg agaaatatag aaaagctgat acagattgag
     4321 cgtaacgcga aagcgcggat acgcagggcg acaaaatcca gaactgaata attaaattta
     4381 gcactgttaa aaatttaatc cttaaccgga gggatttctg caccctcaaa tcatca
//
LOCUS       DAJVRL010000196         4428 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVRL010000196 DAJVRL010000000
VERSION     DAJVRL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.198"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..252
                     /gene="ydcF"
                     /locus_tag="OQN02_003895"
     CDS             <1..252
                     /gene="ydcF"
                     /locus_tag="OQN02_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein"
                     /protein_id="HCS1644508.1"
                     /translation="PQLGNNADSVIFINQLQGLWPVERYLSLLTGELPRLHDDSDGYG
                     PRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            449..1888
                     /gene="aldA"
                     /locus_tag="OQN02_003896"
     CDS             449..1888
                     /gene="aldA"
                     /locus_tag="OQN02_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCS1644509.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(1930..2931)
                     /gene="gap"
                     /locus_tag="OQN02_003897"
     CDS             complement(1930..2931)
                     /gene="gap"
                     /locus_tag="OQN02_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1644510.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3120..3650
                     /gene="cybB"
                     /locus_tag="OQN02_003898"
     CDS             3120..3650
                     /gene="cybB"
                     /locus_tag="OQN02_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1644511.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3895..4068
                     /locus_tag="OQN02_003899"
     CDS             3895..4068
                     /locus_tag="OQN02_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644512.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4140..4289)
                     /gene="hokB"
                     /locus_tag="OQN02_003900"
     CDS             complement(4140..4289)
                     /gene="hokB"
                     /locus_tag="OQN02_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1644513.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1129 a         1007 c         1114 g         1178 t
ORIGIN      
        1 cctcagttag gaaataacgc agacagtgta atctttatta atcagttaca aggattatgg
       61 ccggttgagc gttatctctc actactcact ggcgagctgc cgcgtttaca cgatgatagc
      121 gatggctacg gtccccgcgg gcgagatttt atcgttcacg ttgattttcc ggcagaagtc
      181 atccatgcat ggcaaacgct gaaacatgat gcggtgctca tcgaggcgat ggaaagtcgc
      241 tcgttacgtt aaaaattgcc cgtttgtgaa ccacttgttt gcaaacgggc atgactcctg
      301 acttttattt ctgcctttta ttccttttac acttgttttt atgaagccct ccacagactt
      361 gtcctttccc gatgccgtct ctctgatgat tgatgttaat taacaatata ttcaccgaaa
      421 acaaacatat aaatcacaag agtcgcccat gtcagtaccc gttcaacatc ctatgtatat
      481 cgatggacag tttgttacct ggcgtggaga cgcatggatt gatgtggtaa accctgctac
      541 agaggctgtc atttcccgca tacccgatgg tcaggccgag gatgcccgta aggcaatcga
      601 tgcagcagaa cgtgcacaac cagaatggga agcgttgcct gctattgaac gcgccagttg
      661 gttgtgcaaa atctccgccg ggatccgcga acgcgccagt gaaatcagtg cgctgattgt
      721 tgaagaaggg ggcaagatcc agcagctagc tgaagtcgaa gtggctttta ctgccgacta
      781 tatcgattac atggcggagt gggcacggcg ttacgagggc gagattattc aaagcgatcg
      841 tccgggagaa aatattcttc tgtttaaacg tgcgcttggt gtgactaccg gcattctgcc
      901 gtggaacttt ccgttcttcc tcattgcccg caaaatggct cccgctcttt tgaccggtaa
      961 taccatcgtc attaaaccca gtgaatttac gccaaacaat gcgattgcat tcgccaaaat
     1021 cgtcgatgaa ataggccttc cgcgcggcgt gtttaacctt gtactggggc gtggtgaaac
     1081 cgttgggcaa gaactggcgg gtaacccaaa ggtcgcaatg gtcagtatga caggcagcgt
     1141 ctctgcaggt gagaagatca tggcgactgc ggcgaaaaac atcaccaaag tgtgtctgga
     1201 attgggtggt aaagcaccgg ctatcgtaat ggacgatgcc gatcttgaac tggcagtcaa
     1261 agccatcgtt gattcacgcg tcattaatag tgggcaagtg tgtaactgtg cagaacgtgt
     1321 ttatgtacag aaaggcattt atgatcagtt cgtcaatctg ctgggtgaag cgttgcaggc
     1381 ggttcaattt ggtaaccccg ctgaacgcaa cgacattgcg atggggccgt tgattaacgc
     1441 cgcggcgctg gaaagggttg agcaaaaagt ggcgcgcgca gtagaagaag gggcgagagt
     1501 ggcgttgggt ggcaaagcgg tagaggggaa aggatattat tatccgccga cattgctgct
     1561 ggatgttcgc caggaaatgt cgattatgca tgaggaaacc tttggcccgg tgctgccagt
     1621 tgtcgcattt gacacgctgg aagatgctat ctcaatggct aatgacagtg attacggcct
     1681 gacctcatca atctataccc aaaatctgaa cgtcgcgatg aaagccatta aagggctgaa
     1741 gtttggtgaa acttacatca accgtgaaaa cttcgaagct atgcaaggtt tccacgccgg
     1801 atggcgtaaa tccggtattg gcggcgcaga tggtaaacat ggcctgcatg aatatctgca
     1861 gacccaggtg gtttatttac agtcttaatg agtgaaagag gcggaggttt tttcctccgc
     1921 ctgtgcgcgt cagagtttag cgaatttttc gagggtgcga ataagctgcg tgacgaagcc
     1981 atattcgtta tcgtaccagg cgaccgtttt caccagttgt aaatcgccca cggcggtaat
     2041 ttccgtttgc gtggcatcaa acactgaacc gaaatggctg ccaatgatat cggaagagac
     2101 tatttcttca tcggtataac caaatgactc gttattggtg gttgcttgtt taagtgcgtt
     2161 attcacctct tcggcagtca ctttttttcc gagaatggac accagctcag tgaccgaacc
     2221 tgttttcacc ggcacgcgtt gcgcatgacc tttcagtttg ccgctcagtt ccgggatcac
     2281 cagaccaatg gcttttgccg cccccgtagt gtggggaatg atattttctg ccgctgcgcg
     2341 tgaagcacgt aaatctttac cacgcgggcc atccaccagt gactgggtgc cagtataggc
     2401 atgaatggtc gtcatcgtgc cgacttctat cccgaaactg tcatgcaagg ctttggccat
     2461 cggcgcaagg cagttagtgg tgcatgacgc cacggaaaca atggtgtcgt tgccatccag
     2521 agtgtcgtca ttaacgttat aaacgatggt tttcatttca ccggcaggag cggaaatcaa
     2581 caccttcttc gcaccagcat caagatgcgc ctgcgattta tcggcggagg tataaaagcc
     2641 agtacattcg acaatgattt ctgccccttt cgctttccac ggaatatttt tagcctcttt
     2701 ttcggcgtaa accgcgatac ttttcccatc aacgataagt gaatcttccg taaaatcaac
     2761 gctccagggg aacggaccat agtttgaatc atgtttcagc agataggcga gaatttttgg
     2821 ggaagtaaga tcattaatag cgacaacgtc tatgttgctt ttgacttcaa gtaaacgacg
     2881 caacaccagt cgaccgatac gaccaaaacc gttaatacca actttactca tggttttctc
     2941 ctgtcaggaa cgttcggatg aaaattgatc ctttccaggc ttagaccagg attgtgggat
     3001 gggcaatctc cattcttaca gtgaaacgta acgtactgaa aacgggtgaa caatatttaa
     3061 tgaaattttg agaaaagccc gttatgttaa cggaaaatta tgttaaagca ggaaatgtta
     3121 tggaaaataa atactcaagg ttacaaatca gcattcactg gctggtcttt ttgctggtta
     3181 tcgcagcgta ttgcgcaatg gagtttcgtg gtttcttccc acgtagcgat cggccgctca
     3241 tcaacatgat tcatgtttcc tgtggcatct caattctcgt gctgatggtg gttcgtcttt
     3301 tgttaagact gaaataccca accccgccga ttatacctaa gccgaaaccg atgatgacgg
     3361 gactggcgca tttgggacat ttggtgattt atctcctgtt tattgcgctg ccagtgattg
     3421 gtttggtgat gatgtataac cggggcaacc cgtggtttgc gtttggtttg acgatgcctt
     3481 acgcttcaga ggccaatttc gaacgggttg atagcttaaa gtcgtggcat gagacgctgg
     3541 cgaatctggg atattttgtc atcgggttgc acgctgcggc tgcactggca caccactatt
     3601 tctggaagga caacacactt ctacgcatga tgccgcgtaa acgttcctga aggatattta
     3661 aagaaaacgc ctgtactaaa accgacccgt ggtacaggcg aagaatacgg gtctacatcg
     3721 gaagcgccta ttatatttat ttgtatgaca aataaaacca taatccttgc ccatacgtcc
     3781 atctggctta tttttaatca attcacccga tctttgatct catcaacggt atcaaaataa
     3841 accagcgtat aacgttcaaa tcgcacataa gattcgataa accatggagg ttatatgaaa
     3901 aaactggcac ttattttgtt tatgggaacg cttgtttcct tttatgccga tgccgggcgc
     3961 aaaccctgtt ctggttcgaa aggggggatc tcacactgta cggcaggcgg caaatttgtc
     4021 tgtaatgatg gttctattag tgcatcgaaa aaaacatgca ctaactgaag tgtaaaaggg
     4081 gtgccatgag aaaatgaatc tgctagtcag atgcgcgggg aaaatccccg cgcttgccct
     4141 tacctggacg tgcaggccat gagcgcagca acctccttat caccgtcccg gaaccgcagt
     4201 tcgtagagcg tttgtcgggt caggagtgtg aatgccagaa tcgtaatgca gataatgagc
     4261 agacacacca ccagagggtt gcgcttcata gcctttctcc ttgccggatg gcgggtaaga
     4321 ggctaacaat ctgaattgct tggttcattc gttggcctcg gtttgtactg accccaaaaa
     4381 gttggacagt taaacacgag gcatataggt ctgattccga tattcatt
//
LOCUS       DAJVRL010000197         4340 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVRL010000197 DAJVRL010000000
VERSION     DAJVRL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.200"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="OQN02_003901"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="OQN02_003901"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCS1644514.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="OQN02_003902"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="OQN02_003902"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCS1644515.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1134..1892
                     /gene="tam"
                     /locus_tag="OQN02_003903"
     CDS             1134..1892
                     /gene="tam"
                     /locus_tag="OQN02_003903"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCS1644516.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1896..2810)
                     /locus_tag="OQN02_003904"
     CDS             complement(1896..2810)
                     /locus_tag="OQN02_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCS1644517.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            3011..3199
                     /locus_tag="OQN02_003905"
     CDS             3011..3199
                     /locus_tag="OQN02_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644518.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            3209..>4340
                     /locus_tag="OQN02_003906"
     CDS             join(3209..4025,4025..>4340)
                     /locus_tag="OQN02_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1644519.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAG"
BASE COUNT         1081 a         1053 c         1216 g          990 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact accggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
     1141 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
     1201 tgcttgccag agtgccgctg gagaatgtcg aatatgttgc cgatcttggc tgtggcccag
     1261 gtaacagcac cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcatcgact
     1321 cgtctccggc gatgattgct gaagctcgca gtgctttgcc agactgccag ttcgtggaag
     1381 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     1441 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
     1501 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     1561 gcgaagttgc ttgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     1621 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     1681 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     1741 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     1801 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     1861 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     1921 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     1981 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg ttcttccagt
     2041 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     2101 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     2161 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     2221 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
     2281 gaaagctgtc cattgcggcg ctgaaccgcc aaccattctc ccattattaa caagatacgg
     2341 ttagctggag agttcgaagc cagtacacgc tgaagatctc cagtcttgag ataatgagcc
     2401 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     2461 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctctcgc
     2521 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     2581 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     2641 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaatataaca
     2701 gcaatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagccg
     2761 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     2821 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     2881 caaattacct agaaccacgg cagcctgacg gctgcaaatt gaaaactgcc cctgttcgtc
     2941 ctgttgttgc cgcacgctcc gcagaatatg tatgctctga ggacctgttt tattaaggac
     3001 tcatcctctt atgttgaccc gcaaaagtat agataccgtt ctgctctctg ttggtgctga
     3061 gaagctctca caacgtgaat gggactggat gaagatgctc aaacccatgg acccaccacc
     3121 tgcgatggtc gcggcctcca tcctggagcg ccgtggcgac acagcagccc ttacccggct
     3181 acaggatacc gggggttaat ccgtcctcat gttgtccgct tttactcccc gcccgctgaa
     3241 acgtctgttc acggccaacc agtgctggac atccttcctg gatgcgggcg gtctgcgcga
     3301 tatcgaggtt gaggctgtca caaaaatgct ggcctgcggc acgcggatac tgggcgtgaa
     3361 ggaatacatc tgcgataaac ctgagtgccc ccacgtcaga tacgtcacta actcatgcgg
     3421 cagccgtgcc tgcccgtcct gcggaaaaaa ggccacagac ctgtggatag cgacacagct
     3481 gaatcgtctt cctgactgcg actgggtaca cctggtcttc accctgccgg acacgcagtg
     3541 gccggtgttc gaaagcaacc ggtggctgct gaatgacgtg tgccgtctgg cggtggagaa
     3601 tctgctgtat gccgcccgga aacgggggca ggaacccggt atcttctgcg ccatccacac
     3661 gtatggccgt cgtctcaact ggcatccgca tgtacatgtg tctgtaacct gtggaggtct
     3721 gaataagcat ggtcagtgga aaaagctgag cttcctgaaa gacgcgatgc gttcgcggtg
     3781 gatgtggaat atgcggcaac ggcttctgaa agcgtggtca gaggggctgg cgatgccgga
     3841 gtcgttgtca catatcacga cggaatcaca gaggagaagc ctggtgctga aagccggagg
     3901 aaaatactgg catgtgtaca tgtcgaaaaa aacggccggc gggaggaata cggcgcgcta
     3961 cctgggtcgt tatctgaaga agccgccgat agcggcctcc cggctggcac attacaacgg
     4021 agggggagcc tgagcttccg ttacctggac cacaaaacgg gagaaacggc gacggaaacg
     4081 ctgacacagc gtgagctggt cgcgaggctg aaacagcaca tcccggagaa gttttttaag
     4141 atggtgaggt acttcgggtt ccttgccaac cgggtgtgtg gagagaagct gccgcaggtg
     4201 taccgtgctc tggggatgga taaaccggaa ccagtggcga aagtgtgcta tgcacaaatg
     4261 gtgaaacagt tcctgagtcg tgacccgttc gaatgcgtgc tgtgtggcgg ccggatggta
     4321 taccgccggg ccatcgcggg
//
LOCUS       DAJVRL010000198         4242 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVRL010000198 DAJVRL010000000
VERSION     DAJVRL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.201"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            29..910
                     /gene="yddG"
                     /locus_tag="OQN02_003907"
     CDS             29..910
                     /gene="yddG"
                     /locus_tag="OQN02_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1644520.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1165..2265
                     /gene="ompC"
                     /locus_tag="OQN02_003908"
     CDS             1165..2265
                     /gene="ompC"
                     /locus_tag="OQN02_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1644521.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2429..2914
                     /locus_tag="OQN02_003909"
     CDS             2429..2914
                     /locus_tag="OQN02_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1644522.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3024..4013
                     /locus_tag="OQN02_003910"
     CDS             3024..4013
                     /locus_tag="OQN02_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1644523.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1315 a          875 c          805 g         1247 t
ORIGIN      
        1 taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct
       61 aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct
      121 cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac
      181 ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt
      241 attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca
      301 ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat
      361 tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt
      421 cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa
      481 tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc
      541 ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct
      601 gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt
      661 tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatac
      721 tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt
      781 tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc
      841 gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg
      901 tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa
      961 tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc
     1021 acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc attctatttt
     1081 aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa
     1141 cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt
     1201 ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg taataaactc
     1261 gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg tgacgatggt
     1321 gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt
     1381 ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa cggctcgcgc
     1441 ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg ctctctggac
     1501 tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat gttgccagaa
     1561 tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac caccggtcta
     1621 gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt tgccgcgcaa
     1681 tatcaaggca aaaatgaacg tactgacaac agtcatcttt atggtgctga ctacacgcgt
     1741 gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt tggtatcggt
     1801 gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc taatcctctc
     1861 aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga tgccaacaac
     1921 atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg ggatggttat
     1981 atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga cttcggcttg
     2041 cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag
     2101 gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc
     2161 tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc cgtagatatt
     2221 cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc tctcctgacc
     2281 agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt aagtaaaaat
     2341 gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat atcctccggg
     2401 gacatcttac tataatattt attgatgagt gattatgacc aatattaata cagcttgtgt
     2461 aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa ccatctccag
     2521 caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg gtgaagaaag
     2581 agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat ctcttgattt
     2641 gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata cgttaaatct
     2701 ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg ttaatatcaa
     2761 cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac tggaaaacat
     2821 ttatattgat cataacaatc tggaaagttt agatttaaaa aatcagcatt cattggttaa
     2881 tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat aaactgaaat
     2941 ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa gaatctctga
     3001 ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat catcccagga
     3061 tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa aacactacaa
     3121 aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac attgatcaac
     3181 taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat gatccacttg
     3241 cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat gaagtgacca
     3301 ttgatttatc aggacttaat ttaaccactc aaccacctgg gctgcaaaac ttcacctcta
     3361 tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat agactcgtaa
     3421 agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc agaaatgtaa
     3481 gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata gataggcttt
     3541 catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa ttagaatctt
     3601 gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt gttgcaggta
     3661 ataaaaatga actcttactg ctggatctat cccataataa actaacaagt ttacacaatg
     3721 tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct gaaattaaaa
     3781 tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac cagttgaaat
     3841 atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga ctggacaata
     3901 ataaaataac ccacattgat actaataata catccgatat tggaacttta ttccccataa
     3961 taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat tgacctacta
     4021 tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact ggcctggcaa
     4081 ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat aacgaattgt
     4141 ttcactatac tattaaaaaa gcatataccc catataatat acacacttac aagacagagg
     4201 aagttgtaaa ccagaggaat ataaaagtta aaaacatgac ct
//
LOCUS       DAJVRL010000199         4197 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVRL010000199 DAJVRL010000000
VERSION     DAJVRL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.202"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..157)
                     /locus_tag="OQN02_003911"
     CDS             complement(<1..157)
                     /locus_tag="OQN02_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1644524.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTY"
     gene            complement(307..468)
                     /locus_tag="OQN02_003912"
     CDS             complement(307..468)
                     /locus_tag="OQN02_003912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644525.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(465..653)
                     /locus_tag="OQN02_003913"
     CDS             complement(465..653)
                     /locus_tag="OQN02_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1644526.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            913..1248
                     /gene="iraM"
                     /locus_tag="OQN02_003914"
     CDS             913..1248
                     /gene="iraM"
                     /locus_tag="OQN02_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1644527.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1529..1637)
                     /locus_tag="OQN02_003915"
                     /pseudo
     CDS             complement(1529..1637)
                     /locus_tag="OQN02_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2160..2236)
                     /locus_tag="OQN02_003916"
     tRNA            complement(2160..2236)
                     /locus_tag="OQN02_003916"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Arg,seq:tct)
     gene            complement(2338..2413)
                     /locus_tag="OQN02_003917"
     tRNA            complement(2338..2413)
                     /locus_tag="OQN02_003917"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2378..2380),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="OQN02_003918"
     CDS             complement(2558..3379)
                     /locus_tag="OQN02_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1644528.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="OQN02_003919"
     CDS             complement(3394..3750)
                     /locus_tag="OQN02_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1644529.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..>4197)
                     /locus_tag="OQN02_003920"
     CDS             complement(3763..>4197)
                     /locus_tag="OQN02_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1644530.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPES
                     PESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTL
                     PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1160 a         1136 c          826 g         1075 t
ORIGIN      
        1 gatatgtcag cagcccaaac agcagactcc ccagcacgcc tattgccgcc cactgagacg
       61 gggaaaccct gtccagcaac tgcaggaacc agtagcccgt tcccaccgct gacgtggtgt
      121 atgacacacc tgttgtgatt ttttccatct ggtccatacc ccgtctcccg ttatccggaa
      181 gctgacaaca ataaaaaagc caccagttaa ctactgatgg ctctgataac tcatgcaagc
      241 gtctcagacg acccactgac actaccggtg agtttaacga taccttccat ttgactggct
      301 cactttttat gatgatgccg gtgcatttat ctccagcacc agactttcta tctcaacgcc
      361 atacgttgca tttttggtaa tatccgtcag cgtcagtgca tttagtccca ctgccagact
      421 gtcttttatg gcctggaatg ccgggccagt acgatgacgt agtatcactc cggctcagtt
      481 gcaccactga ccaccacatc accttctgct gcaatcgcct gcatcagggt ataaggggtt
      541 atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt cagcttttcc
      601 gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc catggttctt
      661 tccaacatct gaaaaataat tctttaaaat acctgacatg taatacagaa aaaacacaaa
      721 accatacctt aaataaaaac ctgattatca agcagatatg catggataaa ctacaagacg
      781 agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa taattctgct
      841 ctatggttta attcaaaaat atcatttata cttttcagaa catcaccagc aaggcataaa
      901 caaggaaact aaatgaagtg gattgtgatt gatacagtta tccagccatc atgcggaata
      961 tctttttcag tcatatggag taaaataaaa ttaataatct ggtatcaatc ggatgctttc
     1021 ttacctcctg aaagtatatt tacactgact cacacaggca tcatgctcaa taacaaagtg
     1081 ctacctgtaa ccatttacaa cgtagtacca ttcaataaaa cattctggaa tttaatcaaa
     1141 aacagccagg aatgccctac aaatacagat aacgtattga atgaatgctt taataaccgt
     1201 tgcactctgc aaatatgtcc ttatggacta aaacaacaaa gtccataagg agtttactca
     1261 catctgacaa aatcaatata aacagcccct ccggagaggg gctggagagt ggcgctatgt
     1321 gccattccat ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat
     1381 tatcccatat acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt
     1441 cttttaacaa actccccgca aaccagacaa cagtcaaccg cctgaattgt gaagtattta
     1501 aaaatttctc ccgctaactg atacccggct aacagtctgg cgttttcttt ttcagcaacg
     1561 ggaaagcaac aaccaccaca cccgccacca gcacaccgtc agccggcact gacattatcc
     1621 ggctgctgca atgccattaa caaaaacagt aagcaatcac tttttaccgt aacaggtgat
     1681 aatccagata tgtatctacc ccagatgagt aatccgaagt tcatccatac cacaggtcct
     1741 ggctattctg ttgtactcct gaacaagagc aaataattct gaattagcaa ccatgaactc
     1801 atcgcaaacc ctctgtatag catcactatt cagaataata acgtctcttc ccgaaagacg
     1861 atcaggagta cagaacaaaa ctgtcaaacg gctgaaggcc tttgctcgtg ctgcattgac
     1921 tatatcaata cgctgcctaa ggatgaaaca ccccgacgcc tcatcaatat tcactctacc
     1981 cacaccatat gaatgataaa tacttaatgc tgaaaaaacc attagaccgt ataacaaata
     2041 ctcaatcaat acttaacaga acttttattt ttgacaaata tataatattt tcaacaatat
     2101 cctgagccag gtatatttca gtataaggct ctgccggaag gaatctggaa gaatgaatat
     2161 ggcgcgctgt actggattcg aaccagtgac cgattgctta gaaggcaatt gctctgtccg
     2221 gctgagctaa caacgctgaa taccgataat ggaccgccat cggggacccg ccccccgcac
     2281 caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg tttgtgatgg
     2341 tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact
     2401 gagctaaagg gccgggagca gaataataac ggtccgtaat taatttcgca ataaaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgccagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gatttggtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag cggcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccgg
//
LOCUS       DAJVRL010000200         4183 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVRL010000200 DAJVRL010000000
VERSION     DAJVRL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.203"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQN02_003921"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQN02_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1644531.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OQN02_003922"
     tRNA            complement(324..399)
                     /locus_tag="OQN02_003922"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OQN02_003923"
     tRNA            complement(406..480)
                     /locus_tag="OQN02_003923"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OQN02_003924"
     tRNA            complement(597..681)
                     /locus_tag="OQN02_003924"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OQN02_003925"
     tRNA            complement(690..765)
                     /locus_tag="OQN02_003925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OQN02_003926"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OQN02_003926"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1644532.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQN02_003927"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQN02_003927"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1644533.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQN02_003928"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQN02_003928"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1644534.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1081 a         1039 c          929 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DAJVRL010000201         4168 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVRL010000201 DAJVRL010000000
VERSION     DAJVRL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.204"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OQN02_003929"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="OQN02_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCS1644535.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OQN02_003930"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="OQN02_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OQN02_003931"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="OQN02_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3787..>4168
                     /locus_tag="OQN02_003932"
     CDS             3787..>4168
                     /locus_tag="OQN02_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCS1644536.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1037 a         1208 c         1023 g          900 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacgtag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgccttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAJVRL010000202         3771 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVRL010000202 DAJVRL010000000
VERSION     DAJVRL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.206"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..356
                     /locus_tag="OQN02_003933"
     CDS             <1..356
                     /locus_tag="OQN02_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1644537.1"
                     /translation="FVHTKPAYRSDGIKTAAVRKMRTDSNKAWKGALKRAGISNFRFH
                     DLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNG
                     TNTAQEENVVYLNAR"
     gene            618..980
                     /locus_tag="OQN02_003934"
     CDS             618..980
                     /locus_tag="OQN02_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCS1644538.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            977..1897
                     /locus_tag="OQN02_003935"
     CDS             977..1897
                     /locus_tag="OQN02_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1644539.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1900..2851
                     /locus_tag="OQN02_003936"
                     /pseudo
     CDS             1900..2851
                     /locus_tag="OQN02_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2878..3118
                     /locus_tag="OQN02_003937"
                     /pseudo
     CDS             2878..3118
                     /locus_tag="OQN02_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3115..3420
                     /locus_tag="OQN02_003938"
     CDS             3115..3420
                     /locus_tag="OQN02_003938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644540.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3654..>3771)
                     /locus_tag="OQN02_003939"
     CDS             complement(3654..>3771)
                     /locus_tag="OQN02_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1644541.1"
                     /translation="MATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
BASE COUNT         1100 a          734 c          729 g         1208 t
ORIGIN      
        1 tgtttgtcca caccaaacct gcctaccgaa gcgacggaat aaaaacagca gcggtaagga
       61 agatgagaac cgacagcaac aaggcatgga agggagcgtt aaagcgggca ggcattagca
      121 acttccgctt ccatgacctg aggcatacct gggcaagctg gctggttcag tccggtgtct
      181 ctcttcttgc gcttaaagag atgggaggat gggaaactct cgaaatggtt caaagatacg
      241 cccacctttc agccgggcat ctcaccgagc acgcgagcaa aatcgatgcg attataagtc
      301 gcaatggcac aaatacggca caagaggaga acgtagttta cttaaatgcg aggtaactta
      361 ttgatttaaa tggtgccgat aataggagtc gaacctacga ccttcgcatt acgaattata
      421 agaatccgct tgtaattcaa aggattatcc catcaacact gcgctcacac gtcccaccac
      481 atcaaaacat gtaaagcctt gcaagccatt gtgaggcctt atgtgtctca gttttgtctc
      541 atcagacata gcaagtatcg atcaattgag acttggatga tagacttcat gcctttcaga
      601 gctcattgat taaataaatg ttaaagttat ttgtaaagta cacatctata ggtgtgctga
      661 acacacttat acactgggtg gtttttggtg tatgtatcta taccgcacat accagtcagg
      721 ctctggcaaa cttcacaggt ttcgtagtgg ctgtgagctt tagcttcttc gcgaatgcaa
      781 gattcacatt caaagcatcg actacagcga tgcgctacat gttatacgtg ggatttatgg
      841 gaatattgag tgtgattgtt ggttgggcag ctgataaatg ctcacttcct ccaatagtca
      901 ctcttatcac cttctccgcc atcagtctgg tgtgcggttt cgtctattca aagttcattg
      961 tctttaggga tgcgaaatga aaatatctct tgtcgttcct gtcttcaatg aagaagaagc
     1021 gatacctgtt ttctataaaa cggtacgtga attccaagag ttgaagccat atgaagtaga
     1081 aattgtattc ataaatgacg gaagtaaaga tgccacagag tcaattatta acgcgctggc
     1141 tgtttcagac ccgctagttg ttccgctgtc atttacacgc aactttggta aagaaccagc
     1201 cttatttgca gggttagacc atgcaagcgg cgatgctgta attcctattg atgtcgacct
     1261 gcaagaccca attgaggtta tccctcatct tattgaaaag tggcaggcag gtgctgagat
     1321 ggtgcttgct aaacgttcag accgctcaac tgatggacga ctgaaacgta agacagctga
     1381 gtggttctat aaattacaca acaaaataag caccccaaag atcgaggaaa atgtcggaga
     1441 ttttcgactc atgtctcgtg aggttgtgga gaacattaaa ctgttgcctg agcgcaatct
     1501 tttcatgaaa ggcatactga gctgggtggg tggtcagacg gatgtcgttg aatatgtacg
     1561 cgcagagcgt gttgctggca tctcaaaatt taatggctgg aaattatgga atctggcact
     1621 ggaaggtatc acaagttttt caacctttcc tcttcgcgta tggacttata taggcttgtt
     1681 tgttgcaagc atttcatttt tatatggtgc atggatgatt atagacaccc ttgtctttgg
     1741 taacccagta cgcgggtatc cctccctgct tgtatcaata cttttcttgg gtggagtgca
     1801 actgatcggg attggtgttc tcggtgaata tataggtaga atttacttag aaacaaaatc
     1861 aagacctaag tatattttga agaaaggtag aaaataacca tgagcatatg tattaaacaa
     1921 tcagcattaa aaatattatt ggctttatca gcactcctaa taacttggtt gactaccaga
     1981 tactttccag ttgaaccgga tgttgctaac agcccaattg tatggaggca tattttagag
     2041 aatggtatat catcaatcca cgactggaaa ccaaccgtgg ataattggta tttcaccgtt
     2101 tacccaatta actttttatt ttatatgctg ttaggtgatg atggcttagt agcattaaga
     2161 ttatcaactg cagttttctc tattgctata gtaatagcag cgatgttaac gctacgtaag
     2221 gcattcgggt ttacccctgc tttattttct attatacttc tatctctaat accatatttt
     2281 tcatacactt atggttttgt atcacatcct ttttctcata attcaacaaa cgctttcggc
     2341 tttttatgct tgcttatatc tgttttcaac atacagtata aaaatatatt tatcactctc
     2401 ctcttaagtt tgacagcatt attttccagc gtatccgatc cgtggtttac agctgcattt
     2461 tttattccac tattaatatc ttacttttta ttctctgtat gggataaaaa attatttaag
     2521 catactgcat taatactatt tgcatgttta atctctcttt ccaatgtatt gcaaaatcta
     2581 cttaacatac ctccccacca atttgaaatt gtatcactca atgatatgat tttaaatgca
     2641 aaatggtgca ttctgttaat aggaaaaagt cttaatttat tagttgtcga caataacgca
     2701 acatcttacg cctcatttgt catttggttc attgccatta ttacatctgc gtggtttgtt
     2761 ttatcggaca ataaaaaaaa cacataccgt atttatattg tgttattttc attattatct
     2821 atcgcaggta ttgtctcatc attcatactg agctataaat cccctgatta tattagcatg
     2881 cgtttcttta tgaatgttac ctgtttcgca ctgatattgt gctgtatcgg tacatcaaca
     2941 aaagcaaaga tattatttta tctaatcgca tttttatttt cgatcagttc aattaaatct
     3001 tacacaaata atgcttcacc attacacgat caggagaaga ttgttaaatc atacattgat
     3061 tttttaaaaa aaaataatct ccattatggg tacggctctt tttgggattt gtcaatgact
     3121 gtaaattggt tatctggtgg ggacatacag ataactcctg ttttctttaa tgctgattcg
     3181 gggaaaatta actttactgg cgtgagacaa cagacactgg cttcttggca tagcaaggag
     3241 gcgttcaatt ctgctcctga gcgacagttt atagcagttt ccatagctaa cgaacctgaa
     3301 cgatgtaaag aaatgacatc ctgccttgca ggtattcaag aacaattagg taagcctgac
     3361 gaagttttga actttgaagg aagagtaata ttagtattta acaaaaaact taacctatag
     3421 taaattgccc cctaccatat aaaccacact aacgaatgaa taacccgtct ttttcgacgg
     3481 gttatatccc tatcaggaat aaaaatggag tggttgccgt atacaacaaa ccctcattta
     3541 gagctatatt ctagagtata tgagatacta taaagttgaa ggagctatca ttagaaaaac
     3601 catatgctgt tataattaga tgtctcgctt ttcatccaat aacgaatttt ctcttataga
     3661 ttgctctctg gttttttcgg ccactctgta ttttttatat caacacgcat caatagtgcg
     3721 cgatattttt tccatgccag taacagtttc gtttcatcat ctgttgccat a
//
LOCUS       DAJVRL010000203         3671 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVRL010000203 DAJVRL010000000
VERSION     DAJVRL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.208"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            20..499
                     /locus_tag="OQN02_003940"
     CDS             20..499
                     /locus_tag="OQN02_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644542.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
     gene            487..720
                     /locus_tag="OQN02_003941"
     CDS             487..720
                     /locus_tag="OQN02_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCS1644543.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            720..2186
                     /locus_tag="OQN02_003942"
     CDS             720..2186
                     /locus_tag="OQN02_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCS1644544.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            2077..2820
                     /locus_tag="OQN02_003943"
     CDS             2077..2820
                     /locus_tag="OQN02_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS1644545.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            2824..>3671
                     /locus_tag="OQN02_003944"
     CDS             2824..>3671
                     /locus_tag="OQN02_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1644546.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDN"
BASE COUNT          945 a          903 c         1045 g          778 t
ORIGIN      
        1 gtcttggata gaagccgcag tggatgcgca caaaacgtta aatttcgaac ccagtggaag
       61 aaagcgtatt ggctttgacg tggctgacag tggtacagat aagtgcgcta acgtttaccg
      121 tcacggatcc gttgttttct gggccgacga atggaaggcc aaagaagatg aattactgaa
      181 gagctgccag cgtacttatc aggcggcgct ggagcgtgaa gcagatattg tttacgactc
      241 tatcggtgtt ggtgcgtctg ccggtgctaa attctctgaa attaacgctg accggaagag
      301 cgagaacgca tacgcgcgac gtgtgaatta ccagaggttt aacgccggtg ctggtgtgca
      361 tgagccagat gacgagtaca acggcatccc caacaaagac tttttcgcaa atcttaaggc
      421 tcaggcatgg tggctgaccg tttcagaaat acgtttaacg ccattaacaa cggagaacag
      481 tatcctgtgg atgagctgat cagcatagat tctcgttgtc cgttgcttga aaagctgaaa
      541 ctggaactga caacacctca tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt
      601 aaaaaagacc tcgcaaaacg cgagataccg tcaccaaacg ttgctgatgc attcattatg
      661 gccttcgcgc caatcgatac atcgctggat atctgggaac agctggggag acaggcctga
      721 tggcacgaaa caaacaagcc ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga
      781 attttattgc ccgagtaggg atgcagacac ctaaccagca ctcagcatcc acctaccggg
      841 ctaatttcac cagtcgtaac cgcatgctgg tggaatggtc ctatcgttca tcctggatca
      901 tcggcgaagc agtcgatgct atcccggatg atatgacccg caaaggcatt cgcatcactt
      961 cggaaattga tgcaaaagat cgtggcattc tcgaatcaca actggatgag ttgcaaatct
     1021 gggatgcgct gaatgacgtg ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca
     1081 tcatgattga ggggcaggca ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca
     1141 agtttaaggg gattctcccg ctcgaccgct ggatggtcga cccggcactg acccgccgca
     1201 ttaaagatat ggggccggac ctgggtaaac ctgagtttta cgatgtggtg accacagcaa
     1261 cgggaattcc tgcctggcgc attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt
     1321 tgccatttca gcagaagatg accgagaacg aatggggaat gtcggttgta gagcgtatct
     1381 gggatcgtct taccgcgttc gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg
     1441 cgcatttgcg tacctacagc gtggagaagc tacgcgagct tatcgcactt ggtggtcctg
     1501 cgtatgaagc gttgctgaag aatatcgacc tgattcgaca gttccagagt aatgaaggca
     1561 tgacgctcat ggactcgcgg gataagtttg aaacccatca gtacagcttc agtggtctgg
     1621 atgacatcct ttcgcagttt gcagaacaga ttagtggcgc tgttggtatc ccactggtgc
     1681 ggttgttcgg acagtccccg aaaggatttt ctaccggtga tgcagacctt gccaactatt
     1741 acgatcgcat cagttcgttg caggagaggc gtttacgtct tccggtgcgg cggatactgg
     1801 acatcatgca tcgttcggaa cttggcaagc cgctgccgga cgatttcacg tttgagttta
     1861 acccgctctg gcaaatgtct gatgtcgatc gctcaacggt ggcgttaaac accaccaacg
     1921 caatcagtac ggcgctgggt gatggtctga tgacactgaa agccgctatg actgatttgc
     1981 gagaaaattc tgacgtaacc ggcatagggg catccattac caacgaggac atcgagaatg
     2041 ccgaagatga agcgccgccc ggcatcggcg aacctgatga cgaaccgcag gagccgtcag
     2101 gcggaaatcc ggtatcgaac cagcctacgc aggatagcga gggcggtcgg agacatcgta
     2161 aatggtcgct acgatggttc aaatgacagt atcacggaaa ttattgcggc gctggagcgt
     2221 tacagtgaaa tcatcacccc ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta
     2281 acccggcaga acgagaaagt ttggcggcaa cacagcaaga acatcagtcg cgagctccgc
     2341 aatcttgtgg aaagcgctcc tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc
     2401 aagtacatca aatctctgcc tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa
     2461 gcgatagagg ctgttgtcac cggtggtcgg gcggagcagt ttgctaaaga gattgcatct
     2521 accggcgatg ttgctaagtc tagggccgat ctgattgccc gaacggaact gggaagagca
     2581 acgggcgcgc tggatatggc ccgagcgata gctattggct cggatggtta tatttggcgt
     2641 acagccgatg atggcgatgt ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc
     2701 tgggattcac ctccaacttt ggacggcatg accggccacg cgggcgagct gccaaactgc
     2761 cgctgctata aagagatcgt gtttgttcgc gttccattcg caatgaaaag ggcagcataa
     2821 cccatgaaat acttttttga gaccaggctc ggggaaaccc gataccgcct ggctgacggc
     2881 tcgttgctgt gcaaagacgt gccgatagga cgaacaggta agcagctcta tggtgctgat
     2941 gacctgccaa aactgaaacc cgataagttc gttgaaatag tcgtcacgcg ttctcctgag
     3001 caggtattcc atccggccac gcttgcctca ttcgaaggga tgagcatcac gatcctgcat
     3061 cctgaagatg aaaacgggaa tgtgcggctg gtaaatcccg agaactggaa agtgcttgct
     3121 gtcgggcacc tccagaatgt ccggcgcggg acgggtgagc agtctgattt gatgctggct
     3181 gaccttatcg tcaaagacga aagcgccatt cagcttatcg aagatggcct gcgcgaagtg
     3241 tcgtgcggct atgacgcgga gtacgagcag accgagccat gtaaagccga gcaggtcgat
     3301 attaccggaa accatgtggc tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt
     3361 ggagacagag acacaatggc aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc
     3421 atcaagacag gagatgccga caccatgaac gaactggtgg agtcggctcc cgcatcggtt
     3481 acaggagatg agggggattt gccgcagggc gttaatctca acatcaacct gtccccgcag
     3541 caaccactac cggacaaagc accagagatg ggcggagatc caaccggcga cagtgatgat
     3601 gacctcaaaa cattactgaa agccctgctg gctaagctgg aaagaaatgc cacgggcgat
     3661 aacgataata a
//
LOCUS       DAJVRL010000204         3590 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVRL010000204 DAJVRL010000000
VERSION     DAJVRL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.210"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            32..493
                     /locus_tag="OQN02_003945"
     CDS             32..493
                     /locus_tag="OQN02_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS1644547.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="OQN02_003946"
     CDS             complement(534..1004)
                     /locus_tag="OQN02_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1644548.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="OQN02_003947"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="OQN02_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1644549.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="OQN02_003948"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="OQN02_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1644550.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          953 a          926 c          817 g          894 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DAJVRL010000205         3540 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVRL010000205 DAJVRL010000000
VERSION     DAJVRL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.211"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..455
                     /gene="pgrR"
                     /locus_tag="OQN02_003949"
     CDS             <1..455
                     /gene="pgrR"
                     /locus_tag="OQN02_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1644551.1"
                     /translation="EMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAI
                     NLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVER
                     AIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            792..2405
                     /gene="mppA"
                     /locus_tag="OQN02_003950"
     CDS             792..2405
                     /gene="mppA"
                     /locus_tag="OQN02_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1644552.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2456..3487)
                     /gene="ynaI"
                     /locus_tag="OQN02_003951"
     CDS             complement(2456..3487)
                     /gene="ynaI"
                     /locus_tag="OQN02_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1644553.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          771 c          816 g          947 t
ORIGIN      
        1 gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca atggctattg
       61 ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca caattaatag
      121 atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc tggagattaa
      181 tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg aatacgatag
      241 acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct tatgatcagg
      301 ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc actccagatt
      361 tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca ttctcattat
      421 ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata cattaattta
      481 attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt tattcttcgt
      541 acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc gaagcccgaa
      601 cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt tacactaaat
      661 ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt ttttgatgga
      721 agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta atgtcggtta
      781 gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg gtcagcagca
      841 tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag aagcaggagc
      901 tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc gtgggcctgc
      961 cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac gaaaaagggg
     1021 agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc tggactttta
     1081 ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa gattttgtct
     1141 acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg tttgccgcgc
     1201 tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct gatcagcttg
     1261 gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg ttgccgtggt
     1321 ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac gtagaaagcg
     1381 gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt cttaaagagc
     1441 gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat aacgccaaaa
     1501 cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc actaagcgtt
     1561 acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat cagaagctgt
     1621 tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat tattatgcct
     1681 ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta agtatgacga
     1741 tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca gcgtggcatt
     1801 ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa caaatgagtc
     1861 aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat ggtccgcaaa
     1921 aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa attgcgattg
     1981 ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa aatcaggaat
     2041 ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc gcctcatggg
     2101 tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg cattcaggaa
     2161 atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca tcgacggaaa
     2221 ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc atggagcaag
     2281 caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg tggctgaaag
     2341 gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat attgtgaagc
     2401 attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt ctgatttagc
     2461 gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg aaggcaaagt
     2521 ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc tgtgcagcaa
     2581 gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc aatgacgagt
     2641 cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc gggtgatttt
     2701 tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc tcataacgta
     2761 aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct acgctgatcg
     2821 acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta attttggtaa
     2881 ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa cggatccagt
     2941 cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa ttactcagaa
     3001 tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc agcaagccag
     3061 aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata atggtgattt
     3121 tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta atctgctgtt
     3181 tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca atcagggacg
     3241 tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt tctataacat
     3301 aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat tgcaccgcat
     3361 ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc atcagaatca
     3421 atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta aacagttcag
     3481 cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa cccctggcgg
//
LOCUS       DAJVRL010000206         3494 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVRL010000206 DAJVRL010000000
VERSION     DAJVRL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.212"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(954..1145)
                     /locus_tag="OQN02_003952"
     CDS             complement(954..1145)
                     /locus_tag="OQN02_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1644554.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1231..1614
                     /locus_tag="OQN02_003953"
                     /pseudo
     CDS             1231..1614
                     /locus_tag="OQN02_003953"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1595..2125
                     /locus_tag="OQN02_003954"
                     /pseudo
     CDS             1595..2125
                     /locus_tag="OQN02_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2132..2365
                     /locus_tag="OQN02_003955"
     CDS             2132..2365
                     /locus_tag="OQN02_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1644555.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2911..3378)
                     /locus_tag="OQN02_003956"
     CDS             complement(2911..3378)
                     /locus_tag="OQN02_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644556.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          869 a          796 c          971 g          858 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tggagctaca gaccggaaaa
     1261 cgcctccagc cgtactacga ccgcgcagac aggcctagaa ttgatgcctg gcagacgctg
     1321 gttaatcact attacgggct gcatgacccg aacgccccgg agaaccgcag gacgctgaca
     1381 ctccctgata atctgccgga aacgaaacag gcgattgctg agggcgttac acgcgggata
     1441 gacgcgcttt accatgccgg agagataaaa ggccgtcagg atgtgattca ggctctcaca
     1501 gaggcaggga ttgacgtggt tagggtgacg cgaagcagta tcagcattgc agacccgaac
     1561 ggcgggaaga atatcagact gaaaggagcg ttttatgagc aatcttttac agacgggtgc
     1621 ggagttcgag aaaaagctga aagagagagc cgaatctaca gagaaaatgc tgaacgacga
     1681 gttcaggaag ctcgaagaat ctgtaaacag ggaactgacc tcaaacgaat ctttaatcag
     1741 gaacgctatc aacgaccata catcagccct gaaagaactt ctggagaggt atcagaaaac
     1801 gaccgtcgat acgatggacg cacactggaa aaccgtactg aaaatgagcg tgaaacgctg
     1861 gctgtggctg attatcgtct cagtactgat gtttgccacg accgggagcc tgctgtggta
     1921 ccagggcatg aagataaacg cgaacatgaa tattctccgc gagtagaaag agagtctgga
     1981 gaagctgaac gcgaagacat ggggcgtgcg ttatcacgag gacagcaacg gacgttttct
     2041 ggtgttgccc gaggggatga aagccgaaac gaactggacg atggaaaacg ggaaacagaa
     2101 tgccgtaagg ctggtacagg agtaacagag catgacggag ctggaaagac atttgctgaa
     2161 cgcattagaa caactgcaac aggactatat gcagcggctg aacgaatggg agagcgcctt
     2221 cgtggaattg cagaaaatgt tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg
     2281 ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag cagacagaac gcttgagcca
     2341 gttatacagc gagaacagga gataagggac gaacggctga tacaggaatg cgaacatgcg
     2401 ttatccctgg agcgtgagca ccagctggaa atacaggaac ggacactgga tggcccttcg
     2461 ctgggatggt aatcagagac aggctaaaat gttgtcagcg cgtttccggt gcattttttg
     2521 ggataatggt tggttcgggg gcaccgggaa cgcgcttcag tcagaccttg ctgaaaacat
     2581 aggaacgcgt taaaacgcat acgggcgcga ttctggtgtg tcttttgttg ctgtgttttg
     2641 cctctattgc cagatcgcgc tcattcccct gaacttcttt tttcccgctc aggtccccgt
     2701 cttcttcgct tccgaaagca tgaaaaaaat ccgtttcagc tgtctctggc tggcaattcc
     2761 tgcgtgattc acatggctgc atagctatgc atgtggttaa aatttaccgg gtgcgatcgc
     2821 ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga tcgcgccgaa
     2881 cgcgtttcag cggtgcgtac aattaagata ttacgttaaa ttgtgaggga atggtcccca
     2941 tatactgact ttatcatagg gatagggttg gtagtgggtc gattgggttt cggcattgtt
     3001 tccgttaatg ccaattgcat ttttgtattt gatgcaacag aaggcgtgag attctcctgg
     3061 gcctggaaag gataagtgtt tagaattgat ggcaaaatat cttccatccg atttttgaag
     3121 tagttgttgt ttggctgtct cccaaagggt gttgttacaa acttcttgaa tttgtagttt
     3181 ttgcaggact cttattactg cgttatcatg ttccaattct ttaggggagt gtattattct
     3241 ctcttttaat aagctgttta ccacagggtt tagttgttgt ctaaagcacg cagataatgc
     3301 cattgctaca caattttgtt cccgaggaag catgtttggc ggggtatgcc gtgtttgatg
     3361 tgatatgtct attctcatag aaggtctctg gcttatagca tcaacaaata cggctataaa
     3421 acaaaatttt catagagtga ttcaagatct tcttgagatc cttttttgcg gcgcgtaatc
     3481 tgctgcctgc aaac
//
LOCUS       DAJVRL010000207         3177 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVRL010000207 DAJVRL010000000
VERSION     DAJVRL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.214"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            417..1526
                     /gene="fepE"
                     /locus_tag="OQN02_003957"
     CDS             417..1526
                     /gene="fepE"
                     /locus_tag="OQN02_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1644557.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2551..2745)
                     /locus_tag="OQN02_003958"
                     /pseudo
     CDS             complement(2551..2745)
                     /locus_tag="OQN02_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          800 a          659 c          774 g          944 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgccctggg ggcgctgcta ggggtgtctg ttcagatatg tagcaatgag tcagggattc
      121 acgaactgaa aatgcccgaa aatgtgtctc atgggcactg tatgcgccca aaccgatcgt
      181 cattcacttt catgcatagc tatgcagtga gctgaaagcg atcctgacgc atttttccgg
      241 tttaccccgg ggaaaacatc tctttttgcg gtgtttgcgg cagaatcgcg ttcagcgcgt
      301 tttagcggtg cgcgcaataa aatgttatgt taaatgtctg ctgaatggtt tttaggtttt
      361 ttcctaacat atgatagaat aaaaaaacca tatgttaata tattgtgttt ttttcaatga
      421 aaatctcaaa ggtctctgac gaacattttt ctaattacaa catgttgccg aatggtaatg
      481 aagaaattga cctttttggc ttgattagcg ttttatgggc agcaaaaata aaaattgttg
      541 gggtgattct attttttgct tgtattggct ttcttgtttc ttttgttttg ccacaaaaat
      601 ggactagttc tgctgtggtt actcctgctg agtctgttca atggagtgaa ttaaataagg
      661 agctttccaa gcttcatgtt ctgggagtgg gttttgatat caatcgtgat agcgcatttg
      721 ctctgtttat taagaaattt cagtccgtta attcgttgaa tgagtatatg agatcatctc
      781 cttatgttat ggagattatt aaaaagaata acattagcgc tctcgaactg catcgtgcaa
      841 ttgttgagtt gagtaaaaat atgaagtcga ttgatgataa tgccagtaag aaaaacgaca
      901 agtcatcatt gtatgtatca tggactctga gttttactgc tccaacaagt aaagaagctc
      961 acgatgtgtt gtctgggtat attaattatg tttcttccct tgttgtaagg gatttgatgg
     1021 aagatataag aaataaacta gaagttaaaa ctaatgttga aaaagaaatt cttgcactgg
     1081 atgagataaa aattagaaac cagctgaatg cagatattcg acgcctcaat tattcactgg
     1141 aggttgctaa tgcggctgga ataaaaaaac ctgtatacag caatggtcag attatgaagg
     1201 atgacccaga ttttcctgtg gctctcggtt ctgatggtat agcaactaaa ttgaacatca
     1261 aaaaatcaat caaggatgtt tcggaattga gtggggagtt gcgaaatcgt caatatgttg
     1321 tgaatcaatt ggttgtggcg aaagtggggg atgttgattt tatgccattt cagtatcaat
     1381 tgagcccaac gttaccggtc agaaaggatg ggccaagtaa tgccataatt gtaattcttt
     1441 cttctgtcat tggagggatg cttgcatgtg gctatatact tttacgtcat gccatggagg
     1501 ccagaaagat gagtgtgatt gagtaaaagt tgtgctgatg catcatttta taagcaactt
     1561 ttataaaaag ttcaaaggat cttcttgaga tcattccctt acagccgtag tcttctactc
     1621 tagaaaacga aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac
     1681 ttctgaaccg tggtaacagt cttgtgcgag acatgtcacc aaatctgtcc ttctagtgta
     1741 gcctcagtaa ggctgccact tcaagaactc tcaatacatc tctcgcacat cctgtttacc
     1801 agtggccgct gccaatggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1921 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacgaacgc ggccataaca
     1981 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2041 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccaccg gcgcggcctt
     2161 ttttcggggc ctctcttttg ctcacatgtt ttttctggtg ttatcccctg attctgtgga
     2221 taaccgtata tccgtctttg cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg
     2281 cagcgagtca gtgagcgagg aagcggaaga gcgccggaag ctgcattttc tccttacgta
     2341 tctgtgcagc atttcacacc tcatatgtcg ctgttctctc accatctgct ctgccgcctc
     2401 ataattaagc cagtatacac tccgttatcg ctacgtgact gggtcatggc tgcgccccga
     2461 cacccgctaa aaccccctga cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg
     2521 ggaaagccgt gaccgcctcc gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa
     2581 gcgaatctgg atgctgttct ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc
     2641 cgcctgttcg tcggcatcca gagcatccag tttttcaagc agtgtcaggc tctggctttt
     2701 gatacatcgc gccatattca gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc
     2761 gtcagttccg ggtgtcgggg ggggaccctg agcaggtggt aattgtaaga cgttgcgggc
     2821 gatggtatta caattgcaca tcctgtcctg tttctttagg acgtatcatg gcacagcata
     2881 tcaactgata aataattcct gtgcagtgca gccgctgttg cataaaattt cgctctgcgt
     2941 cgagtgtttc ttaatcaata attccgctga tactccggaa aaacgcgagt ctgttctcat
     3001 gcctgaaatg cccacacccc acgcaaaaac aagtttttgc tgatttttct ttataaatag
     3061 agagttatga caaattagtt cttcttgctc tctttgtgat atttaaaaaa gcggtgtcgg
     3121 cgcggtgttg tagctgcgcc aacaccgctt tttaggggtg gtactgacta ttttcat
//
LOCUS       DAJVRL010000208         3134 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVRL010000208 DAJVRL010000000
VERSION     DAJVRL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.215"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..68)
                     /locus_tag="OQN02_003959"
     CDS             complement(<1..68)
                     /locus_tag="OQN02_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644558.1"
                     /translation="MASISISCPSCSATEGVVRNGK"
     gene            260..1237
                     /locus_tag="OQN02_003960"
     CDS             260..1237
                     /locus_tag="OQN02_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1644559.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(1285..1548)
                     /locus_tag="OQN02_003961"
     CDS             complement(1285..1548)
                     /locus_tag="OQN02_003961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644560.1"
                     /translation="MLPEQHAAEARYPIPVEDFHFSIFTAVNNQTVMTSSAEIVLVIE
                     GTATLSHASGESLLLHVGLSAFIPASIGRWLLTTTGKVCRVSC"
     gene            complement(1583..>3134)
                     /locus_tag="OQN02_003962"
     CDS             complement(1583..>3134)
                     /locus_tag="OQN02_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HCS1644561.1"
                     /translation="DIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYL
                     EERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLP
                     RIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPG
                     LTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRG
                     ESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIE
                     EHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNI
                     KHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILD
                     WLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAI
                     VLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFR
                     PLRMPGKP"
BASE COUNT          595 a          950 c          987 g          602 t
ORIGIN      
        1 cttttaccgt tacgcaccac gccttcagta gctgagcagg agggacaact gatggagatg
       61 gaagccacgg gagcacctca aaaccaccat catacactaa atcagtaagt tggcagcatc
      121 accgtaatcg tacagctaag aacaagtgct tcaagtctac ttattaaaat gtacctttga
      181 aaaagtagac tttctgcggt acgctctgac agtaagtttg cccccgattt tttacgcagc
      241 gcgccggacg gagcccgaca tgatcctgat tcccgatgac gaaccggagc tgagtctgcc
      301 cgcagccagt gaggaattcc tgccggcgct gtcaggtgag aatgccccgg tcagcccggc
      361 ccgggcctac ctgctttcac tcaattccca ccgcagccgg cagaccatgg cctcgttcct
      421 gaacatcgtc gccgttatgc tcggagctgc ctccctggag tcctgcagct ggggcagcct
      481 gcggcgccat cacgtgatgg ccgtgaccga actgctgcgc gacaccggcc gggccaccgc
      541 caccgtcaac acctatcttt cggcactcaa gggcgtggcg aaggaagcct ggatgctccg
      601 gctcatggat gtggagagct tccagcatat ccgggcggtc cgtaacctgc gcggcagtcg
      661 gctgcccagc ggtcgggcgc tgccccaggg ggagatccgc gccctgtttg ccgtctgtga
      721 agccgatcgc agctgcctcg gggcgcggga tgcagcgatg ctggcggtca ttctcggctg
      781 cggcctgcga cgatccgaag tagtgagcct ggatttgcgt gacgttgtca ctcaggaccg
      841 tgcgcttaga gtgctgggta agggaaacaa ggagcgactg gcatatgtcc cggccggtgc
      901 ctggcagcgg ctgcagatct ggatcgatga gatccggggc gagaccccgg gcccgctgtt
      961 cacacgcatc cgtcgtttcg gggatgtgac tctaaaccgg ctaaccgacc aggcggtgta
     1021 ccatatcctg caggtgcgcc agggtcaggc cggcatcacg aaatgttctc cacacgatct
     1081 gaggcgaacc ttcgccaccg cgatgctgga taacggggag gatttaatta ccgtgaagga
     1141 tgcgatgggc cacgcgagcg tcaccaccac ccagcagtat gaccgtcgcg gggagcaacg
     1201 cctgcaggac gcgcgggata gactcaacct tatttagggc taagtaaaca caaacaaggg
     1261 ggctgtaccc cctctaattt tacattaaca cgatactcga cagactttcc cagtcgtagt
     1321 cagcaaccat cgcccgatgg aggctgggat aaatgctgac agcccgacgt gaagcaaaag
     1381 gctctcgccg ctggcatggc ttaatgtcgc ggtcccctca atcactaaca caatttcagc
     1441 gctggatgtc attaccgttt gattgttcac cgcagtgaaa atactgaaat ggaaatcctc
     1501 aacaggaata ggatagcgcg cttctgcggc gtgctgttcc ggtaacatag gggagcccgc
     1561 agaattcgga aaaaatcgta cgctaaggtt ttccgggcat ccgtaagggc cgaaacttcc
     1621 cgtcttccag tctgcggctc tgccgccaga cgtaatcgcc ggttaggttg atgtgctccc
     1681 agcccagcgg cgacaggaat tgcagcagct cgccgtccac cggcttgccg gcctcgacca
     1741 acccctgggt ggcgcgttcc aggtacaccg tgttccacag cacgatagcc gccgtcacca
     1801 ggttgaggcc gctggcccgg tagcgctgct gctcgaagct ccgatccctg atttccccaa
     1861 ggcggttgaa gaacaccgcc ctggccagcg agttgcgcgc ctcacctttg ttcaggccgg
     1921 catgcacgcg gcggcgcagt tcaacacttt gcagccagtc caggatgaac agcgtgcgct
     1981 cgatccggcc cagctcgcgc agggccacgg ccagtccgtt ctggcgcggg tagctgccga
     2041 gcttgcgcag catcagcgag gcggtgacgg tgccctgctt gatcgagctg gccaggcgca
     2101 ggatgtcgtc ccagtgggca cgcacgtgct tgatgttcag ggtgccgccg atcagcgggc
     2161 gcaacgtcgg gtaggcttgc acgccctgcg gcacgtacag cttggtttcg ccgaggtcgc
     2221 ggatgcgcgg cgcgaagcgg aagcctagca ggtgcatcag ggcaaagacg tgatcggtga
     2281 agccggccgt gtcggtgtag tgctcctcga tccgcaggtc ggactcgtgg tacagcaggc
     2341 cgtcgagcac ataggtggaa tcgcggacgc cgacattcac cacgcgggtg ctgaacggcg
     2401 cgtactggtc ggagatatgg gtatagaaca gccgtcccgg ctcgctaccg tacttcgggt
     2461 tgacgtgccc ggtgctctcg ccccggccac ccgcgcggaa gcgctggcca tcggaggatg
     2521 aggtcgtgcc gtcgccccag tgggcggcaa aggcgtggcg atactggtgg ttgaccagct
     2581 cggccaaggc cgccgaatag gtttcgtcgc ggatgtgcca ggcttgcagc caggacagct
     2641 tggcgtaggt caggccgggg ctcgactcgg ccatcttggt cagcccgagg ttgatcgcat
     2701 caccgaggat tgcggacagc agcaacgtcc tgtctttggc ctcggccccg tccttcaagt
     2761 gggtgaagtg gcggctgaag cccgtccagt cgtccacgtc catcagcagt tcggtgatct
     2821 tgatgcgcgg cagtaactgg ctggtttggt cgatcagcgc ctgcgcccga tccggcaccg
     2881 ccgcatccag cggggtgatt ttcagccctg actcggtgag gatggcatcg ggcagctcgt
     2941 tgtccttggc caggcgggtg acggtggcca actgctcgtc cagcagctgc aaacgctctt
     3001 ccaggtactg gtcgctgttc gggttgatcg ccaggggcag ggcctgctcg cgcttgagtg
     3061 cggcgaactt ctcggccggc agcaggtagt cgtcgaagtc gcggaactgc cgcgagccct
     3121 tgacccagat gtcg
//
LOCUS       DAJVRL010000209         3131 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVRL010000209 DAJVRL010000000
VERSION     DAJVRL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.216"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            32..2935
                     /locus_tag="OQN02_003963"
     rRNA            32..2935
                     /locus_tag="OQN02_003963"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3031..>3131
                     /gene="rrf"
                     /locus_tag="OQN02_003964"
     rRNA            3031..>3131
                     /gene="rrf"
                     /locus_tag="OQN02_003964"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          826 a          687 c          979 g          639 t
ORIGIN      
        1 atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta cacggtggat
       61 gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga
      121 tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt cgtcacacta
      181 tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca tctaagtacc
      241 ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg ggagcagccc
      301 agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg atacagggtg
      361 acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg ggacacgtgg
      421 tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga ctgaccgata
      481 gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt gaaaaagaac
      541 ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg cgtacctttt
      601 gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg gagccgaagg
      661 gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc cggtgatcta
      721 gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga ctaatgttga
      781 aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg agatagctgg
      841 ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg tagagcactg
      901 tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat accggagaat
      961 gttattacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga aacaacccag
     1021 accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga aggcccagac
     1081 agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag ctcactggtc
     1141 gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct gcggcagcga
     1201 cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt gctgtgaggt
     1261 atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg ggtgaaaagc
     1321 ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg tgagtcgacc
     1381 cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc tgtacttggt
     1441 gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg tcccggttta
     1501 agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg tgatgacgag
     1561 gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta agcatcaggt
     1621 aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc aaggcgcttg
     1681 agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag aaggcacgct
     1741 gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata ccagctggct
     1801 gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta tacggtgtga
     1861 cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc tcttgatcga
     1921 agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat tccttgtcgg
     1981 gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac ccgagactca
     2041 gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa agaccccgtg
     2101 aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata ggtgggaggc
     2161 tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc tttaatgttt
     2221 gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg tagtttgact
     2281 ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat cctggtcgga
     2341 catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac ggcgcgagca
     2401 ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat cgctcaacgg
     2461 ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat cgacggcggt
     2521 gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc caagggtatg
     2581 gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag acagttcggt
     2641 ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg agaggaccgg
     2701 agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg tagctaaatg
     2761 cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat gagttctccc
     2821 tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg ccgggtgtgt
     2881 aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta accttacaac
     2941 gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat taaatcagaa
     3001 cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt ggtcccacct
     3061 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     3121 catgcgagag t
//
LOCUS       DAJVRL010000210         3103 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVRL010000210 DAJVRL010000000
VERSION     DAJVRL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.217"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..281)
                     /locus_tag="OQN02_003965"
     CDS             complement(<1..281)
                     /locus_tag="OQN02_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat1"
                     /protein_id="HCS1644562.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHT"
     gene            complement(376..849)
                     /locus_tag="OQN02_003966"
     CDS             complement(376..849)
                     /locus_tag="OQN02_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1644563.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1181..1717
                     /locus_tag="OQN02_003967"
     CDS             1181..1717
                     /locus_tag="OQN02_003967"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCS1644564.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            1832..2158
                     /locus_tag="OQN02_003968"
     CDS             1832..2158
                     /locus_tag="OQN02_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1644565.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(2346..2585)
                     /locus_tag="OQN02_003969"
     CDS             complement(2346..2585)
                     /locus_tag="OQN02_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1644566.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          878 a          679 c          645 g          901 t
ORIGIN      
        1 gtgtgcgaca caacaatgtg ttcgatagag gctagatcgt tccatgttga gttgagttca
       61 atcttcccga caagctcttg gtcgatgaat gcgccatagc aagcagagtc ttcatcagag
      121 tcatcatccg agatgtaatc cttccggtag gggctcacac ttctggtaga tagttcaaag
      181 ccttggtcgg ataggtgcac atcgaacact tcacgaacaa tgaaatggtt ctcagcatcc
      241 aatgtttccg ccacctgctc agggatcacc gaaatcttca tatgacgcct aacgcctggc
      301 acagcggatc gcaaacctgg cgcggctttt ggtacaaaag gcgtgacagg tttgcgaatc
      361 cgttgctgcc acttgttaac ccttttgcca gatttggtaa ctataattta tgttagaggc
      421 gaagtcttgg gtaaaaactg gcctaaaatt gctggggatt tcaggaaagt aaacatcacc
      481 ttccggctcg atgtctattg tagatatatg tagtgtatct acttgatcga tcaggctttt
      541 gtatatctcc ccaccacctg aaacaatgac atgatccgtt attttcttta ggttggttaa
      601 agcatcttta attgatggaa agatcaatac gttctcattg tcagatgtaa aacttgaacg
      661 tgttacgacc gcatactttc ggttgggtaa tgctcccatt gattcaaaag tcttgcgtcc
      721 aaccaacagc cattggttat aggtaatagc tttaaacagg agctgttcac ctttggcact
      781 ccatggaata tcagggccat tcccgataac tccattcttc gatatagcta ccattagtga
      841 tagtttcaca attcttcctc agaggttaac attttattag taagcatgct cgttttgtca
      901 cccgctgatg cttaccgtta attaatataa tcacttgtat taaatagact ttctttaaaa
      961 atacaagaca ctctgttatt acaaatcgtg catgccgtct atcctgcttg cacgatgcac
     1021 attatctcac ccaaagaact cataacattt taatttattt agcattaaat taaaactcta
     1081 ccggtaaaca agcatctcta ggcgtaaaaa acaggcactt ttttaaatcc cagctagaat
     1141 aggctgtata ggcagacagt tgcaagacaa ggagcgtttt atgtctaaca gtccattttt
     1201 aaattctata cgcacggata tgcgacaaaa aggttatgcg ctgaaaactg aaaaaactta
     1261 cctgcactgg attaagcgtt ttattctgtt tcacaaaaaa cgtcatcctc agaccatggg
     1321 cagtgaagag gtcaggctgt ttttatccag cttagcaaac agcagacatg tagccataaa
     1381 cacgcagaaa atcgctttaa atgccctagc ttttttgtac aacaggtttt tacaacagcc
     1441 gttgggcgat attgattata tccctgcaag caagcctaga cggctaccct ctgttatctc
     1501 tgcaaatgaa gtgcaacgca ttttgcaggt tatggatact cgcaaccaag ttatttttac
     1561 gctgctgtat ggtgcaggtt tgcgcattaa tgaatgcttg cgtttgcggg ttaaagattt
     1621 tgattttgat aatggctgca tcactgtgca tgacggtaag ggtgggaaaa gcagaaacag
     1681 cctactgccc acgcgcctaa tcccagcaat aaaataactc attgagcaag cgcggcttat
     1741 tcagcaagac gacaacttac aaggcgtagg gccatcgctg ccttttgctt tagatcacaa
     1801 atacccttct gcttatcgac aagcggcgtg gatgtttgtc tttccctcca gcacgctctg
     1861 caaccacccg tataacggca aattatgccg ccatcatctg catgactccg ttgcgcgaaa
     1921 ggcattgaag gcagccgtac aaaaagcagg catcgttagc aagcgtgtca cttgtcatac
     1981 atttcgtcac tcgtttgcta cgcatctatt acaagcgggg cgtgatattc gcactgtgca
     2041 agaactctta gggcataacg atgttaagac cacgcaaatc tatacgcatg tgttgggtca
     2101 gcattttgcc ggcaccacca gtcctgcgga tggactgatg ctacttatca atcagtaaaa
     2161 gtagataaat atctttatgt agccctgagt agcccctgat actttttatg gtgaaaaaga
     2221 taaacaatca tagaggggat aaatatgagc cagttctacc attgttcaga taagtttaag
     2281 caagatactg tcaatcacag tgaccattca tggtcattac acttcctcca gttgccagac
     2341 aatgcctaac ataaaaacgc aaaagcccaa aagtcagagc ttaggtgttc taggcgtttt
     2401 ctaagcgctg aagaaatacg tcttaccgta gattgcgtgc gtttctgaag cacattttcc
     2461 ttatacatgg ctttatccca ggcagcttca tcaagctctt ggagtaacaa gccagtaata
     2521 atgcgacact ctcgggtttg tagcgagccg ctggtgagat cggagttata gcggaaaggg
     2581 gtcatttgta attatttgtg ggtggaggat agttaactta ttattgtagt tggaagaggc
     2641 tgctttccaa acattagcac ctcgtaatgc ttgttactgt atattaattt atgctcttag
     2701 cgcatgttaa tgggctatta caatgcataa tgtccacatc aagataactg tggcttaaca
     2761 cctgtacaag cgaaaaaaat caatcacctg tccggaaata cgggatcact gtaaagtgtt
     2821 ggtgaggcag tctaggaggg ggatttaatt tgggatactg atgaaaggat tatgatcagt
     2881 tggatatagg atcgtactaa gctgagcggc aggttagcta gattagttaa ccagataagt
     2941 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     3001 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     3061 ccctcccagt tcccaactat tttgtccgcc cacagggctg ccg
//
LOCUS       DAJVRL010000211         3064 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVRL010000211 DAJVRL010000000
VERSION     DAJVRL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.218"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            542..1708
                     /gene="nhaA"
                     /locus_tag="OQN02_003970"
     CDS             542..1708
                     /gene="nhaA"
                     /locus_tag="OQN02_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1644567.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1774..2673
                     /gene="nhaR"
                     /locus_tag="OQN02_003971"
     CDS             1774..2673
                     /gene="nhaR"
                     /locus_tag="OQN02_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1644568.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2711..>3064)
                     /locus_tag="OQN02_003972"
     CDS             complement(2711..>3064)
                     /locus_tag="OQN02_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1644569.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          720 a          678 c          753 g          913 t
ORIGIN      
        1 atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc
       61 tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc
      121 gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt
      181 ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat cgcaattatt
      241 gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa
      301 attaattatt acttttccta attaatccct caggaatcct caccttaagc tatgattatc
      361 tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg
      421 cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa
      481 cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa
      541 agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat
      601 tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga
      661 ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct
      721 gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa
      781 acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc
      841 cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc
      901 gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt
      961 actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc
     1021 tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc
     1081 gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg
     1141 tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt
     1201 gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa
     1261 agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc
     1321 gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac
     1381 gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa
     1441 accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc
     1501 tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac
     1561 tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg
     1621 ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt
     1681 acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct
     1741 cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg
     1801 tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat
     1861 ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa
     1921 ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc
     1981 tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa
     2041 gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt
     2101 agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc
     2161 acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac
     2221 tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc
     2281 gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa
     2341 cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt
     2401 aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa
     2461 gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt
     2521 tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat
     2581 gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat
     2641 tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg
     2701 gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg
     2761 agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2821 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2881 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2941 atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg
     3001 gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact
     3061 gata
//
LOCUS       DAJVRL010000212         3009 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVRL010000212 DAJVRL010000000
VERSION     DAJVRL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.220"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="OQN02_003973"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="OQN02_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1644570.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="OQN02_003974"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="OQN02_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1644571.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>3009)
                     /gene="tnaB"
                     /locus_tag="OQN02_003975"
     CDS             complement(1929..>3009)
                     /gene="tnaB"
                     /locus_tag="OQN02_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCS1644572.1"
                     /translation="GLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYIL
                     TYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGL
                     KIISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLI
                     ICYGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSF
                     LGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFL
                     PPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPA
                     IVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          884 a          767 c          760 g          598 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcctga ggagattatc
     2401 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2461 accagcgcca gcagcgaacc aaataccacg cttttgatta acttatcttt gcgtttgcca
     2521 tagcaaataa tcaggctggg aatattgccg tggaaaccaa atgacgccag acacaccggc
     2581 aaagccataa agatatacgg gaagtaagac gttcccgcag tggtgctggt ggcatcgcgc
     2641 aggatggagt aatcgacctg gaagaagaaa gaaccaaaca cgatcacaaa ggagataatc
     2701 ttcagcccga ggaacaatga ggtaatgcga ctggcggcta acgaacttat ccacaatacg
     2761 ctggcaacga aaatggctgt gcagatcccg acaatacgtg gattagcgtg atagcccaaa
     2821 ttcattgata tcgtttcact aatgatcgca ccattagcag agatataggc ataagtgagg
     2881 atatagagaa cgaaggcaac ggtaataccg ctgataatgt tccaggtgtt acctattaaa
     2941 tctttggtga tggtgttaaa actggagccg acaggataat ttaaatttgc ttctaataac
     3001 aataacccg
//
LOCUS       DAJVRL010000213         2690 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVRL010000213 DAJVRL010000000
VERSION     DAJVRL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.221"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            230..769
                     /locus_tag="OQN02_003976"
     CDS             230..769
                     /locus_tag="OQN02_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1644573.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OQN02_003977"
     CDS             complement(995..1318)
                     /locus_tag="OQN02_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCS1644574.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OQN02_003978"
     CDS             1604..1798
                     /locus_tag="OQN02_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1644575.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJVRL010000214         2572 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVRL010000214 DAJVRL010000000
VERSION     DAJVRL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.222"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(64..822)
                     /locus_tag="OQN02_003979"
     CDS             complement(64..822)
                     /locus_tag="OQN02_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1644576.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OQN02_003980"
     CDS             complement(830..1933)
                     /locus_tag="OQN02_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1644577.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="OQN02_003981"
     CDS             complement(1943..>2572)
                     /locus_tag="OQN02_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1644578.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          710 a          682 c          602 g          578 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggtggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DAJVRL010000215         2521 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVRL010000215 DAJVRL010000000
VERSION     DAJVRL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.224"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OQN02_003982"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OQN02_003982"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1644579.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..>2521)
                     /gene="glnE"
                     /locus_tag="OQN02_003983"
     CDS             complement(1632..>2521)
                     /gene="glnE"
                     /locus_tag="OQN02_003983"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1644580.1"
                     /translation="AMIDAVVQQAWGQMVARYGKPNHLNEREGRGFAVVGYGKLGGWE
                     LGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDA
                     RLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQLTAHFDAVRREIM
                     TLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIEFITQYLVLRYAHE
                     KPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHLALQELPGHVSEDC
                     FTAERDLVRASWQKWLVEE"
BASE COUNT          546 a          774 c          612 g          589 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 t
//
LOCUS       DAJVRL010000216         2488 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVRL010000216 DAJVRL010000000
VERSION     DAJVRL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.225"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(183..656)
                     /locus_tag="OQN02_003984"
     CDS             complement(183..656)
                     /locus_tag="OQN02_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCS1644581.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            812..1825
                     /gene="intI1"
                     /locus_tag="OQN02_003985"
     CDS             812..1825
                     /gene="intI1"
                     /locus_tag="OQN02_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1644582.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2218..>2488
                     /locus_tag="OQN02_003986"
     CDS             2218..>2488
                     /locus_tag="OQN02_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1644583.1"
                     /translation="MKIGYARKSTHLQDVAHQVDELTKAGCEQIYQDQTSRSGPKRDK
                     KGAPELENCLKALREGDTLVVWALDRLGGSLGQVITLLDDLKKRGI"
BASE COUNT          575 a          677 c          649 g          587 t
ORIGIN      
        1 tcctgcgaat atgtgagttt tgacctaatg agccacaaaa actcattttc gcccacgata
       61 atttttctac ttcctttctt tggaatgctc atttcaatat tcacataacg tcgctatcaa
      121 tcgcggaaaa ggcgtagtgc gcgaagcgaa cgaagctttt tgccgtcgat tgcatagctt
      181 tgttaaccct ttttccaaat ttgatagcaa tagttaatgt ttgaagtaaa gtgttgctca
      241 aaaacaactt cgaaggtatt tggaatactc gggaagaaaa catccccctc tggctcgatg
      301 tcgatcgtcg ataagtggag cgtagaggcc atgggtaatg tttctcggta aatttctccg
      361 ccaccagaca ctataacgtg accggtgaat tcagctagcc tgtccatggc ctcttcgatt
      421 gactgaaata caactacatt gtcatcattt gatgtccaac ctgagcgggt aacgaccgcg
      481 tatttcctat tggggagtgc gcccatagat tcaaacgtct tgcgacccac cagaagccac
      541 tgattgtagg tcaatgcttt aaaaagtagc tgctcccctt tcgcggacca gggtatgtct
      601 ggaccgcaac caatcacgcc gtttttcgct ttcgcagcca tcaatgatac tttcaaggtt
      661 ctcatcctgg gttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
      721 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
      781 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
      841 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
      901 tttacgaacc gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt
      961 gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa
     1021 cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta
     1081 cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc
     1141 gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga
     1201 aggcgagcat cgtttgttcg cccagcttct gtatggaacg ggcatgcgga tcagtgaggg
     1261 tttgcaactg cgggtcaagg atctggattt cgatcacggc acgatcatcg tgcgggaggg
     1321 caagggctcc aaggatcggg ccttgatgtt acccgagagc ttggcaccca gcctgcgcga
     1381 gcagctgtcg cgtgcacggg catggtggct gaaggaccag gccgagggcc gcagcggcgt
     1441 tgcgcttccc gacgcccttg agcggaagta tccgcgcgcc gggcattcct ggccgtggtt
     1501 ctgggttttt gcgcagcaca cgcattcgac cgatccacgg agcggtgtcg tgcgtcgcca
     1561 tcacatgtat gaccagacct ttcagcgcgc cttcaaacgt gccgtagaac aagcaggcat
     1621 cacgaagccc gccacaccgc acaccctccg ccactcgttc gcgacggcct tgctccgcag
     1681 cggttacgac attcgaaccg tgcaggatct gctcggccat tccgacgtct ctacgacgat
     1741 gatttacacg catgtgctga aagttggcgg tgccggagtg cgctcaccgc ttgatgcgct
     1801 gccgcccctc actagtgaga ggtagggcag cgcaagtcaa tcctggcgga ttcactaccc
     1861 ctgcgcgaag gccatcggtg ccgcatcgaa cggccggttg cggaaagtcc tccctgcgtc
     1921 cgctgatggc cggcagcagc ccgtcgttgc ctgatggatc caacccctcc gctgctatag
     1981 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacaaac agccagaaag
     2041 gctgttacag gcgatttgat ctgcaaccta ttggttaaat taatgtatca aaaacgatgg
     2101 tttttgtgac agtcttgaaa agtcctgact tctcccgaaa aatgactccc ctcatgtaac
     2161 aaaactcgtt actgtatcaa cataacaata accccataac taattagcga gaaaagaatg
     2221 aaaatcggct atgcacgtaa atctacgcat cttcaggatg tggcgcacca ggttgacgaa
     2281 ctaacaaaag ctggatgtga gcaaatctat caggatcaga cctcacgtag cggcccaaag
     2341 cgcgacaaaa aaggtgcgcc ggaactggag aactgcctga aagcattgcg cgagggagat
     2401 accctagtag tttgggcact agatcggctc ggcggttcat tgggtcaagt cattacttta
     2461 ctcgacgacc tgaagaaacg cggaataa
//
LOCUS       DAJVRL010000217         2451 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVRL010000217 DAJVRL010000000
VERSION     DAJVRL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.226"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            83..706
                     /locus_tag="OQN02_003987"
     CDS             83..706
                     /locus_tag="OQN02_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1644584.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
     gene            complement(760..1932)
                     /locus_tag="OQN02_003988"
     CDS             complement(760..1932)
                     /locus_tag="OQN02_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1644585.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2281..>2451)
                     /locus_tag="OQN02_003989"
     CDS             complement(2281..>2451)
                     /locus_tag="OQN02_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1644586.1"
                     /translation="SVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNL
                     SQSRNKEGTNDV"
BASE COUNT          742 a          446 c          506 g          757 t
ORIGIN      
        1 ttgtgaagga aatggtactc agggggattc cagtacaggt ggtgcgcaat tagctataaa
       61 tggccttaac ggttcgtgcg ctttggtatt aaacaaccca tactttgagg ctaatgccgg
      121 gggggcagac cttgctatag ataatactgg gacgcgccct gtaactgtgg ttataaacgg
      181 tggaaatttt catcgcgtga gcagtgcaag atacacgcac acgaatattc aagtaacaag
      241 ctcaggtggg ggaaaagtta cagtcatact caacggcaca acgttccaga gcgcagggga
      301 ttatcagcca agtgcagaac gaccatactg gataacagga gctaattgcg aggtagttga
      361 cattgggtgt gtttttacag aaacaaccag taaggtaaca tcggcttctg cattgtcagt
      421 cacacgctcg ggaaaaatta acgccaatgg tagtatcgac gttgctactg gtgtttcatc
      481 ggttaacgtg gtttctacag gtgtgtatga tgttagcttt tctcatccgc tcgcagcagt
      541 tgctagcggt tatattgtgc agataacacc tatatctgcg cctgattcag ttagttgtga
      601 tgttacttat attggcgtag atacatttag ggtgaccctg cgcaatacgc ttagcggggc
      661 aggaatctcc agttcgtttg catttagcat taccagacta atataaatac attcgccgcc
      721 ccatttggac atatatgtag ggcggcttat gtttattgtt tattgattgt tgtttgttaa
      781 tgtgggtatt aattctttta ctaaagttgt ggtttgtttt gttaatttga tgaatggcgt
      841 ctctattaat ttaaaagtta ggcatgatgt gaacgtaata agaaatgcag ttgtgcttac
      901 cagtatgata aatgtatttt cagtgtaatt attaggaata atccacgtca ttaggcaata
      961 aaggaatatc ccatgcagaa ggtaaacgct gtaggtagtt tctcctagct ttctggttat
     1021 atttaatctt aatattccat acaaatcaca acctgatgcg ataatgataa atgttattcc
     1081 acaaagaatc agtggtagcg gtgcgtaagt tgttttgaaa tacgtcattt caaaaaccat
     1141 tatcgcagtt attataattg gtgttacttt tgctttggca attccattga ctatctttga
     1201 tttatttagc aaaaatgcca ggagcccaaa aagaaatgat gctatgtgta ttttactgaa
     1261 aaataaaatg aaaacaaata gcgatattgc gctaatcata accatgtaaa tactgacttt
     1321 tctttttatg agcgcggaaa ttaccggaag agaaaaataa aagaaccatt catatacaag
     1381 tgtccatgtt acagcggcat tgatagtaaa tgaatctttt acgtcattaa tgttcggcat
     1441 acctagcgca gtgaatggca accacttcat tattgacaca aaaagctctt ctgtggatac
     1501 ctgcattctc catccagact taaaacctac aatgataaaa atgagacata ggctaactat
     1561 gaacattggg gttaatcgta gtaatcttga tacataaagc cttgtccagt cctgatctcc
     1621 agagataatc tttgaaaaga acagataacc agtaatcata aaaaagaatg acacaccaac
     1681 ttggcctaaa tttgctaaca gatttgatga aggtgcttcc catactccag atgacaagta
     1741 tccattccaa attgctgcgt gatgaataag cacgaaaatt gctaagaacc cccgcattcc
     1801 atctatttga ttattcctgc cgtgctcaac aagatttatt ggggaaattt tatcaaataa
     1861 ccccacagct aataacgaca tcaatatcac ggatgttatt aataataaac tgttaatttc
     1921 aatcatatgc atgctctatt acgcaattaa aatttctcca agtatactga cttttagtta
     1981 atgatcatat agttatgtgt aactgatgaa acaaaacgga gacacaccaa gctttgcact
     2041 ggattgcaag gctttgtgct cttctgtgaa tgtgtggcta tatgtttgaa gatcgttgtg
     2101 ccgtatttgt gacatatata tggcaacatc atgcatcaac tttctgtttg tgtcatcaac
     2161 tatagcttag tgaatgcggt taatgcttgc taaaacagat agttatgatt ggtgctacag
     2221 attcgtaatg cgaaggtcgt aggttcgact gctattatcg gcaccattta aatcaataag
     2281 ttacacatca ttagtacctt ccttatttct tgactgggac aaatttggga ccgatgggtt
     2341 cagaatcaag tctatttgcc gtgcgtgttc ggtaaggtga ttaggtgcga ggtgagcata
     2401 tcgacgaacc atttcgatag actcccagcc tcccatttcc tgtaacactg a
//
LOCUS       DAJVRL010000218         2441 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVRL010000218 DAJVRL010000000
VERSION     DAJVRL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.227"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..566
                     /locus_tag="OQN02_003990"
     CDS             <1..566
                     /locus_tag="OQN02_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1644587.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            524..1201
                     /gene="bglJ"
                     /locus_tag="OQN02_003991"
     CDS             524..1201
                     /gene="bglJ"
                     /locus_tag="OQN02_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS1644588.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1239..2027)
                     /gene="fhuF"
                     /locus_tag="OQN02_003992"
     CDS             complement(1239..2027)
                     /gene="fhuF"
                     /locus_tag="OQN02_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1644589.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2168..2404
                     /locus_tag="OQN02_003993"
     CDS             2168..2404
                     /locus_tag="OQN02_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1644590.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          536 c          627 g          650 t
ORIGIN      
        1 tagtcattgc tgatttagcc ggtgaaagtg aagatccacg ttctgtttgt gaacattatt
       61 attctttaat ctcacaatat cgggaaattc actgggtttt catggtgtca cgctcctggt
      121 attcccaggc tgtagaactg ctcatgtgcc ctacggcgac gttattgtct gatgttgaac
      181 ccattgagaa tctggtcaag accgtacgtt ccggcaatac gcacgcagag cgtatcagcg
      241 ccatgctgac ctctccggca atgactgaaa ctcatgattt tagctatcgc tccgtcattc
      301 tcactctttc agagcgcaag gtactgcggc tattaggtaa aggatggggc atcaaccaga
      361 tagcttcatt gcttaagaaa agtaataaaa ctatcagcgc ccaaaaaaac agtgcgatgc
      421 gtcgactggc aattcacagc aacgctgaaa tgtatgcatg gataaatagc gcgcagggtg
      481 caagagaact taacttgcct tctgtttatg gagatgccgc agaatggaac acagccgaat
      541 taagaagaga aatgtcgcac tcatagaaaa atgcgtcatg agtagtatcg gtattgagag
      601 tttatccaga aagtttgcgg gcaaccctta taagctccat acctatacca gtcaggagtc
      661 atttcaggat gccatgtcgc ggatctcgtt tgcggcggtc attttttctt tttctgccat
      721 gagaagtgag cgcagagagg gattatcttg cctgactgag ctggcgatta agtttccgcg
      781 tacccggcgt ttagttattg tggatgatga tattgaagct cggctgattg gttcattgtc
      841 gccatcaccg ctggacggtg tattaagtaa agcgtcaacg ctggagattt ttcatcagga
      901 actctttttg tcattaaatg gtgtacgtca ggcgaccgac cgactgaaca atcagtggta
      961 cattaaccaa agccggacgt taagcccgac ggagagagaa atattgcgct ttatgtcgcg
     1021 tggctactca atgacacaaa ttgccgagca gcttaaacgt aatatcaaaa cgatccgagc
     1081 gcataaattt aatgtgatgt cgaaactggg cgtcagttct gatgcagggt tgttggaggc
     1141 cgcagatatt ctgttatgta tgcggcattg cgaagcaagt aatgtgttgc atccctatta
     1201 atccgcatga tgccgggttt acttccccgg cagtgctttc atttcagcgt acaatagcca
     1261 cattgctgca catccggtaa gcgataacgc tggcagcaag tgcggcgcac cagcaggccg
     1321 tcgcgcagta ccacggtacg ccagagtgga ttatcttcac cgttcgtgag cgttttctca
     1381 aaaaagaggg catggcgcag cgattcaaca gtagcctcgc cgagcagttg cttcatctca
     1441 gtgagatacc agttgatcaa ataaccggta ttactccaga taagtttgcc gttgatctct
     1501 ccggtcgctt ctagtgcttg cacaaccgga accagcgcct ggctgattaa cgtttccatt
     1561 cgctgctgcg gcgaatgtgg tgttgcgttt ttatcttcac acacatcgac ccagaaacag
     1621 gcgacgcgtc cggtttcgtg aaactcagca tggaaatgtt ccggcgacac atctaatgct
     1681 ttttcctgcg tcagtagcgc cagcattaat ggtggcacca tcaggccgat ataccattgt
     1741 gcccatagtg agatcagcgg tttgttctcg cggatcatcg ttggttggtt gcgatagata
     1801 tgatcggaat agaccgccag tagagaactt agcgcattcg gtgatgacca ttgcgccagc
     1861 gtcatggcgt taagcggagc gggttcatcc aggcggataa actccagcaa atgttcacga
     1921 tgtttggcga tcatcgcccg tacggcttgt gcaagcccag catcctgcgg ctggagatgc
     1981 gttcgccaga tgacatcttc atagagcggt gcggaacgat aggccataat cgtgatagta
     2041 atctaaatga taatgattgc taatcctagc gataggttta ctcgatagca agggatttct
     2101 ctggcttgca aatgataaaa attatcatat gatattggtt ttcattatca atgaaagaga
     2161 tgaaaccatg ttgcaacgta cgctgggcag tggctgggga gtgttgctgc cgggattgct
     2221 gattgcaggg ctgatgtatg cggatttatc gccggatcag tggcggattg tcattctgat
     2281 gggattagta ttgacgccgg tgatgctgta tcacaaacag ttgcggcatt acattttgct
     2341 accatcgtgc ctggcgctta ttgctggcat catgctgatg ataatgaatt tgaatcaggg
     2401 atgacaaaac agggaagaaa caagaaagga agtaaagata a
//
LOCUS       DAJVRL010000219         2432 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVRL010000219 DAJVRL010000000
VERSION     DAJVRL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.228"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            65..1543
                     /locus_tag="OQN02_003994"
                     /pseudo
     CDS             65..1543
                     /locus_tag="OQN02_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2432
                     /locus_tag="OQN02_003995"
     CDS             1540..>2432
                     /locus_tag="OQN02_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1644591.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT          629 a          578 c          603 g          622 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatacctca ttcactgctg gtttttcgcg acaataaggg tctgcatcgt
     2281 tatccctttg gtcaattatc aggcaaagcg atacccgtag atgatgacgt ctcttttgaa
     2341 gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc ccgaccgtca gtggagcaag
     2401 tggatagacc taccaggtat tgaacctgaa ca
//
LOCUS       DAJVRL010000220         2372 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVRL010000220 DAJVRL010000000
VERSION     DAJVRL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.229"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..823)
                     /locus_tag="OQN02_003996"
     CDS             complement(<1..823)
                     /locus_tag="OQN02_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1644592.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1423..1671)
                     /locus_tag="OQN02_003997"
     CDS             complement(1423..1671)
                     /locus_tag="OQN02_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1644593.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2026..>2372)
                     /locus_tag="OQN02_003998"
     CDS             complement(2026..>2372)
                     /locus_tag="OQN02_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1644594.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          610 a          437 c          576 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggtaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttaggtaa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg caatttttcc ttaaggtatg aaaaaaggag
     1081 ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta tataaaaaac
     1141 tgtgttaagg aggctgtcca ttagcggttt tttattcttc tggaaccgcg gcatctttgc
     1201 gtatcttttt gtcttctcac cgtctggtcg gtgttttgct gagactgcta atttcctgtc
     1261 tttgctagtg ctgtactggt actgcccaat catgattggc gggggcgggc ggagttgaga
     1321 ccgcagacac gtcgtatgca agaacgtgct gcggctggct ggtgaacttc cgatagtgcg
     1381 agtattgaat gatttccagc cgttactgat tttacgtgct aattagtgaa caaaccactc
     1441 gtcagcagac tcccaggtat ctttcagagt ttcctgaaca aaagttttag ctgaatcttt
     1501 atcggcggtg cgcgtaacag aaaggccatc gttgctggtg gcttttacga tcacctctac
     1561 atcgtcataa cgtttactga tgcgtcgggt taattcttcc tttaacgcat ccacagcacc
     1621 ggttggcatt ttagtcattt tctctttggc tatgcagatt tcaaaacgca taaaaatccc
     1681 tctgcactgt gtttttatac agtattattt ttaactgtat ggataaacag tgtcaagagt
     1741 gagggaaagt gattttttgg ggcatgcatg gggcaaaaat atttgttgtg gggcattttg
     1801 gggcatgatt gggacacttt gttacatatg aactttgccg attttcatat gacatggcaa
     1861 tccaacgctt tgattattct aaagaaaaca catgctcttg ggcgttcttt agtgattttt
     1921 aaaaatcccg tatcacgcag ttaaagtggc gggcatactc ttcaaggctg gtgatcccca
     1981 accgcaccca tttaggatgt gaccattgcg gtagaccgat gtaaatcata acgcgtgaag
     2041 gatctcttcc acgctacgca cacgggcgat gcgcgggtag atatgattaa tgctgttatt
     2101 gtgttgctcg gcgctggcgg cactacaggc gtcttcggca atcaccagat taaagccaag
     2161 ttcccaggca ttgcgggcgg tggattcaac accgatattg gtcgagatcc cacataacac
     2221 tattgtatcg atacctcggc ggcgcagttg caactccaga tccgtaccgt aaaacgcacc
     2281 ccactgacgt ttgatgattt cgatatcact gtcagttgca cctaatgcag ctggatgttg
     2341 ccaccagttt tcgggcaaca ctttagcggg gg
//
LOCUS       DAJVRL010000221         2233 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVRL010000221 DAJVRL010000000
VERSION     DAJVRL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.230"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            53..2233
                     /locus_tag="OQN02_003999"
                     /pseudo
     CDS             53..2233
                     /locus_tag="OQN02_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          598 a          497 c          562 g          576 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccactcaa tgagtgggca tcgcggcgca acagccctga agacttttgg gaaacaggta
     1501 accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat tatggcaatt
     1561 tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa cgattaatgc
     1621 aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca
     1681 atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct
     1741 acatgctctc tttatctgtt cctttatcgg gctggtgggg taatagttac gccacctatt
     1801 ctgtttcgca aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc
     1861 tggaaagaaa taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg
     1921 ttggcggtaa tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata
     1981 actacagcca aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca
     2041 gcgaaggggt aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg
     2101 gggccgccgg gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata
     2161 cggtcaagac acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt
     2221 tctcgaagtc gaa
//
LOCUS       DAJVRL010000222         2177 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVRL010000222 DAJVRL010000000
VERSION     DAJVRL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.231"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            187..480
                     /locus_tag="OQN02_004000"
     CDS             187..480
                     /locus_tag="OQN02_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1644595.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OQN02_004001"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OQN02_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCS1644596.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..>2177)
                     /locus_tag="OQN02_004002"
     CDS             complement(1922..>2177)
                     /locus_tag="OQN02_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1198 family protein"
                     /protein_id="HCS1644597.1"
                     /translation="SHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKARLSAPITDA
                     QVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
BASE COUNT          661 a          566 c          517 g          433 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgac
//
LOCUS       DAJVRL010000223         2129 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVRL010000223 DAJVRL010000000
VERSION     DAJVRL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.232"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(121..1989)
                     /gene="kup"
                     /locus_tag="OQN02_004003"
     CDS             complement(121..1989)
                     /gene="kup"
                     /locus_tag="OQN02_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS1644598.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
BASE COUNT          618 a          573 c          507 g          431 t
ORIGIN      
        1 tgactgagtt gggaaaacca tcgtgacgtt acggaaatgt gatcaacctc gaaacgtttt
       61 acatggtgat taaccatgaa aacaaaaacg cccccttatg aagaaaggag gcgtctggcg
      121 ttagatttcg acctgagtac ccagttcgat aaccctgttt ggcgggattt caaattgatc
      181 tggtgcacgc agcgcattac gttgcagcag caagtacagc ttgccgcgca gacgcaaata
      241 ccacgggcgt ttgccgagga tcaacgactc atgcgacata aagaaggagg tttccatcat
      301 ccggcaactt aatccttcca gaccgcagcg gtggaaaact tcttccacgt ttggcgtttc
      361 tcgccaacca taacttgcca ccacgcgcca gaaagtgggc gacagttgtt caatctgtac
      421 ccgacggacg ttatggacat atggcgcgtc ttcggtgcgc agagttaaca gaatcacccg
      481 ctcatgcaat accttgttat gtttaaggtt atgcatcagc gcaaagggaa tgacgttgat
      541 tgcacgcgac atatacaccg cggtcccggg cacgcgaaca ggcggtgatt tctccagcga
      601 agcaatcatc gcttccagag agttaccatg ttcatgcatc cgccgcagca agcggaaacg
      661 ctcgctcttc caggtggtca tcacgataaa catcacagta ccgaggctca atggcaacca
      721 gccgccggag agcagtttat cgaggttagc ggtgaacaat ggaatatcga cacaaaggaa
      781 agcaatcagg atcagcgcaa caaaatactt attccagtgc cagttctgac gtgccacggt
      841 agtcgagaga atagacgtca gtaccatggt tccggtcacc gcaatcccgt acgccgccgc
      901 caggttgctg gagtgctcaa agctgacaat cacaatcacg accgcgacat agagcatcca
      961 gttcacaaac ggaatataga tttgccctga ctccatttcg gaggtgtgaa taatgcgcat
     1021 cggcgacaaa tatcccagac gtaccgcctg acgcgtcaat gagaagacgc cagagataac
     1081 cgcctgcgag gcaattaccg tcgccagtgc ggcgatgatc agcagtggga tcagcgccca
     1141 gtccggtgcc aacaggaaga acgggttctt aatcgcttcc gggttcttta acaacaacgc
     1201 tccctgaccg aagtaattaa gggtcaagga aggtaatacg acggtgaacc acgccaggcg
     1261 aatagggaac ttaccaaagt gccccatatc agcatacagc acctcgcccc ccgtaatcga
     1321 cagcaccact gcccctaatg caataaaaga aaccgttttg tattcaagga agaaatgcac
     1381 cgcccacatc ggattcagcg catgcagcac ttccgggtta gcaataatgc tacgtaaccc
     1441 cagtcctgcc agaatcaaaa accaggtcag catgatcggc gcaaacagct taccgaccat
     1501 agccgtgcca tgcttttgaa tcataaataa taaagtgaga acgataattg agaggggaac
     1561 tatccaggta tccagctgcg gggcgacgat ttccagacct tcaatggctg acatcaccga
     1621 aatagcgggt gttatgacga cttcaccata gaaaaagctg ccgccgatta gccccataat
     1681 caccagcatt gatgtggttc gcgccgacgt attacgcccg gcaagcgaca tcaacgtcag
     1741 gatccccccc tcaccggcgt tatctgcccg catcacgaag gtgagatatt taatggaaac
     1801 cacaaagatt agcagccaga agatcagcga taaaaagcca aacacggcat cgcgttcaac
     1861 gccaaaacca aactggccgg acaaacattc acgaagtgta tataacgggc tggtaccaat
     1921 atcgccgtag acaactccaa tcgccgcgag ggtaatcgcg ggcaatgatt gcttattatc
     1981 agtgctcata gactagtctt tcgttgaaat atgaaatgtg tgcttagtcc cttggcccac
     2041 aaaaaagcgc acagtatgca cgattaacgg caaaatcgta ctcctaaatg cggccacatt
     2101 aacctggctc aaagaaaaag ccccccctt
//
LOCUS       DAJVRL010000224         2107 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVRL010000224 DAJVRL010000000
VERSION     DAJVRL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.233"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            32..211
                     /locus_tag="OQN02_004004"
     CDS             32..211
                     /locus_tag="OQN02_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644599.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            263..457
                     /locus_tag="OQN02_004005"
     CDS             263..457
                     /locus_tag="OQN02_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644600.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1256..1592)
                     /locus_tag="OQN02_004006"
                     /pseudo
     CDS             complement(1256..1592)
                     /locus_tag="OQN02_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          677 a          434 c          429 g          567 t
ORIGIN      
        1 actacagcgc attgctttac gagtaataac aatggattta tcaccagaag acgcaaagtt
       61 tttacgagaa ctgcaacacc gtatgctcag agaagccatt atcgaacagc aaacgggcat
      121 gagcgccata gacccattgc gatacaaacg ggcctcatct cctttagttg acgacaattc
      181 caatgcggat aaaacacgtc caccacactg attacatttt tcattagcac agtggtgtga
      241 tgcagcatac gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg agcaactgtt
      301 acaggcacgc caacgggagt ttgccactaa atggggacgt gccgcgctgc cacatgaacg
      361 cctaacctat tttccacttg aagacctcat taaaaataat ggcaaaaaaa tcaggtctga
      421 taagaaacac ttcttagaat gcaatgaaga caaataacaa caatctgcat attgccacca
      481 accacagatg tggcatcgtt atcagcgcac ttgattttct ttatattcac tgacgagctt
      541 gccggggcac ctttgtttgc cggtcattct ggtatcatgc agatatttac ggcgtacaag
      601 cccactagca tgacattgta ataaggcctg agcctaacgc tttaatgtgt gtcatacact
      661 cagtggcgta atcgttgaca atatggtgca tgtaatcgta agcgtccagc gaaatccatc
      721 tggtcataat ctgaagcatc cgacaaagtg gtgtccacca aataagtgat ctgcgcccaa
      781 tatcctgagc cggaactttg acaaaggcca acgaaaaatg ggagcggatg ttacagagtt
      841 ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc gatctgttta accgggaaat
      901 tatctcctac agcttgtcag aaaggtcggt gatggagcat cgttaatacg atgctatacg
      961 atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc agggttggca
     1021 atatcgaatc gcaggctata ggcaaagtta aagccccatg gagtagcaca aaatatgccg
     1081 cacaaaggaa acggtctgaa taacgcagtg atgaagaact tcttcagcac actgctaaaa
     1141 cgcatagtga tcgagcgctg attctggcga acaactgaac taatacatca gaatatgcat
     1201 tatgttaaat aaatatgaaa agatggttta aataccccgt tacttttgac ttacactata
     1261 cggtatcgca tcgtttaata ttcgcaccgg ccagattttt atttctatta gttgtcacaa
     1321 tactgaatgc gtacgaccac agtattctgg ctcctgtgtg gttatgcttt aattctgcgt
     1381 tccgggcaga taagcagttg cttgcaggaa tccttcttgt gttaatgtca gttccccttt
     1441 ttaccagtgc tgatttccac attccgtcca acagagctta tagcctttcc ctggattata
     1501 gcattgtccg gctgaagttc tttttgaata ataatagaag cactgctggc agatccagtc
     1561 cgtttttcat aacccactgt actgataacc ataatctaat cagtagaaat tgagtcgaaa
     1621 ataagcacta ctccatacag gataattaga ggtcagtttg attattcaca attcatcatc
     1681 agcattttct atttctgacg aaatcaacat gaaaataacc atatatgata attattataa
     1741 taacggcttt aattggaata catatattac aacgtattat atataattgg tattctggga
     1801 actatattct caaaatatag tagaaacgag gtatgtttct ggtggaaagg acagtgggat
     1861 taaaaagtaa gaattgataa aaaaaacgcc agcaacacat agtgccggcg gagggaatac
     1921 cccatggaga aaatgtgatg cctagagcat catgaatata taattaaaaa tagttagcgt
     1981 tgtcaatact ttcacaaaaa taattttcgt agtatagaaa gatattttta tgcatgacct
     2041 acctgaattt gctccgggta gaggttataa ataaaaattg aatcacgaca aacacaatat
     2101 tcatagt
//
LOCUS       DAJVRL010000225         2097 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVRL010000225 DAJVRL010000000
VERSION     DAJVRL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.234"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            117..1154
                     /gene="curA"
                     /locus_tag="OQN02_004007"
     CDS             117..1154
                     /gene="curA"
                     /locus_tag="OQN02_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1644601.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1352..2017
                     /gene="mcbR"
                     /locus_tag="OQN02_004008"
     CDS             1352..2017
                     /gene="mcbR"
                     /locus_tag="OQN02_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS1644602.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          561 g          515 t
ORIGIN      
        1 ggattcctcg atgaaattat tgcgtgttgc agaggggatg tgacggctgc aaacaaaacc
       61 aatcccgtga gcctgctact attgatggat tcatgccaaa agcacaggag agcattatgg
      121 ggcaacaaaa gcagcgtaat cgacgttggg ttctggcctc gcgtccacat ggcgcaccgg
      181 ttccggagaa tttccgtctt gaagaagatg atgtcgccac accgggtgaa ggacaggtgt
      241 tactgcgcac agtttatttg tccctggacc cgtatatgcg tggacgtatg agcgatgagc
      301 catcttattc accgccggtt gatattggcg gcgtgatggt cggcggtacg gtgagccgtg
      361 tcgtggagtc gaatcatcct gattatcagc ctggcgactg ggtgctgggc tacagtggat
      421 ggcaggacta tgacatatcc agtggtgatg acctggtgaa acttggcgat catccgcaaa
      481 atccatcgtg gtcgctgggt gtgctgggga tgccaggctt taccgcttat atggggctgc
      541 tggatatcgg tcagcctaaa gagggcgaaa cgttggtggt agctgcggcg acagaaccag
      601 tgggggcgac ggtggggcaa atcggcaaac ttaaaggttg cagagtggtg ggggtagccg
      661 gtggcgcgga aaaatgccgc catgctaccg aggtgttagg cttcgatgtt tgccttgatc
      721 accacgcgga cgattttgcc gaacaactgg cgaaagcgtg cccaaaaggt attgatatct
      781 attatgaaaa cgtgggcggt aaggtatttg atgcagtgct accattactt aatacatctg
      841 cgcgcattcc cgtttgtggc ttagtgagca gctataacgc tacagagcta ccacccggtc
      901 cggatcgttt acccctgttg atggcaacgg tgctgaaaaa acgtattcgc ttgcaagggt
      961 ttattatcgc tcaggattat ggtcaccgca tccatgagtt tcagaaggag atggggcaat
     1021 gggtgaaaga ggataaaatc cactaccgcg aagatattac tgatggttta gagaatgcgc
     1081 cacagacgtt tatcggcctg ctgaagggta aaaacttcgg caaagtggtg atccgcgtcg
     1141 cgggtgatga ttaaagtttc aacggcggcg taagccgcca taaaaatcca ggcaaaaagc
     1201 ttattttcag ctttaattaa ctaaccacgt cattgttctg ctgctaataa caacaaaggt
     1261 gggtggttta tgattattca ctttaataca ctaggtgaac tccttctgcc atgcaggcag
     1321 ggttggacag aaaacacgcg aaggaattaa aatgcctgga acgggaaaaa tgaaacatgt
     1381 cagtttgact ctgcaggttg agaacgacct gaaacatcag cttagtattg gcgcattaaa
     1441 acctggcgca cgcctgatta ctaaaaatct ggcggagcaa ttaggtatga gtattacacc
     1501 tgtgcgtgaa gcattattac gtctggtttc ggtgaatgcg ctttctgtcg cacctgcaca
     1561 agcatttaca gtaccggaag tgaggcaaag tcagttagac gaaatcaatc ggatccgcta
     1621 cgaactggaa ttaatggcag ttgccctggc tgttgaaaac ctctcctcgc aagacatagc
     1681 agaacttcag gaattgctcg ggaaattaca gcaggcgcaa gaaaagggcg acatggaaca
     1741 aatcattaat gtgaacaggc tatttcgctt agcgatttat catcgctcaa atatgctcat
     1801 cctgtgtgag atgattgagc aactgtggat caggatggga cctgctttac attatcttta
     1861 tgaagcgatt aacccagcgg aattacggga gcgcatagaa aactatcatc tattactcgc
     1921 tgcgttaaaa gcaaaagaca aagagggatg caaacattgt cttgctgaaa ttatgcaaca
     1981 aaatattgct attttatacc agcaatacaa tcgttaaatt aaatgcccgg caaataccgg
     2041 gcatcaagta attactcaaa tcgccacgca atattcatgc cgacaccata atttcgt
//
LOCUS       DAJVRL010000226         2091 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVRL010000226 DAJVRL010000000
VERSION     DAJVRL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.235"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            6..224
                     /locus_tag="OQN02_004009"
     CDS             6..224
                     /locus_tag="OQN02_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCS1644603.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OQN02_004010"
     CDS             226..591
                     /locus_tag="OQN02_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1644604.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="OQN02_004011"
     CDS             588..1253
                     /locus_tag="OQN02_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644605.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OQN02_004012"
     CDS             1253..1618
                     /locus_tag="OQN02_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1644606.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          577 a          432 c          559 g          523 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg a
//
LOCUS       DAJVRL010000227         2091 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVRL010000227 DAJVRL010000000
VERSION     DAJVRL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.236"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..224)
                     /locus_tag="OQN02_004013"
     CDS             complement(<1..224)
                     /locus_tag="OQN02_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1644607.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADA"
     gene            complement(221..754)
                     /locus_tag="OQN02_004014"
     CDS             complement(221..754)
                     /locus_tag="OQN02_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1644608.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(820..1449)
                     /locus_tag="OQN02_004015"
     CDS             complement(820..1449)
                     /locus_tag="OQN02_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644609.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1453..1668)
                     /locus_tag="OQN02_004016"
     CDS             complement(1453..1668)
                     /locus_tag="OQN02_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1644610.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(1961..2035)
                     /locus_tag="OQN02_004017"
     tRNA            complement(1961..2035)
                     /locus_tag="OQN02_004017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2000..2002),aa:Gly,seq:tcc)
BASE COUNT          543 a          553 c          459 g          536 t
ORIGIN      
        1 gcatcagcta actccttcgt gtatttagca tcgagtgcat cagcatcacg ctggcgctgc
       61 tgcatgtcag taatggttgc gttcgccagc ttcagttctc cggcgttttt gtcgcgctgc
      121 tctttgtagg taatggcgtt atcacggtaa tgattaacag cccatgacag gcagacgatg
      181 atgcagataa ccagagcgga gataatcgcg gtgactctgc tcattgatct atcccccaac
      241 aggctaatgc gctttcctgg tcacgacgaa taacctgtcc atagcagtta tttgaacgta
      301 tgcggcaatc gcgcccaaca tcttttatcc accagcgaat cgcctcgcat gcgcccttac
      361 gatcaccggc attcagccgc ttataaaacg tcgacgggaa acacttaccg gggccaatgt
      421 tataggggca aaatgacgct atacccgctt tctggggttc ggtcagaggt actttaatat
      481 tgcgctccac ccatgccagc gccttatcac gttcaatagc gttaacctgg tcacattttt
      541 ccttcgacag cttcattccc ggtatgacag gcttaccatc caccattgtg gcaccacgac
      601 agatggtcca tatgccagaa ccatcacggt atgccgttgt gtgattaccc tctttttcgt
      661 tcagaaactg gtcaagtatc tggggagcag acgcgcctgc ggcaatcagc gccagaacag
      721 cagccgacag gccgtatttg atttttgcgc tcatggatat ttatcaggat ttatcggcaa
      781 caaataacga gccaacttat atacgccttt taagataagt cagccccggc tggatccagt
      841 catcaggctc tttcttaaag ggggagtatt gaaatcacga agaagagcct cccgcaccgc
      901 agcatccatg tctgcaccac tggcaagtgc ttcaatctct gccgccacct gcaaataccc
      961 catgcaacgg ccaatgcgtt tcataactct ggtgttttcg tctttcaggc atctgatagc
     1021 gagattaata attttcaggc agtgatgatg cgatgcccgg acaggttttc cgttctcata
     1081 aacgacgaac tcaccctctt cacaggaaac cctgaaaggc cgatggggtt cctggtcttt
     1141 atccggtgca gacgcaataa aatcactttt atgctcttcc tctccggcac cgttaaccgt
     1201 aatgacgtac tcaggccgca ggtaagccgc ctctttttct gacaatgcgg ccggtaaaaa
     1261 cttttcctgc aaaaacttca taaaagtctc gtgagcgact ttgtggcagt aatcgtaaat
     1321 cgccgcataa tgctcatcgc ggcgtttgtt ttcatcttca gaactcacca acgccgacaa
     1381 ttttttatta agctcagtga tttcattttc caggtgattg aatcgctgat tcatttcttc
     1441 atgcttcatt atttactctc cccgtgcagc cttacgcttg tcttctctta ttttgaaata
     1501 cagattcgtc agataagtca gaagccccag aagcagactt cccagcacac caatcgcagc
     1561 ccactgtgat ggcctgacct gatccaacca ctgcaaaaac cagtagccgg cactgccggc
     1621 ggaggtgccg taggcaatgc ccgttgaaat tttgtccatg gatttcatag cctcacctcc
     1681 gcaaataacg gatggcgtag ttttacactg agaaatgaaa gggatttgaa aagaaaaaaa
     1741 cgcaaaagcg ggcgaaacga tatatacagt aaggaaagca ctctatccaa caaaccaccc
     1801 acagttaatc ggaataaaag cagagtgctt atgaatgatc gcacgcccga aggttagtat
     1861 ttctgcacag caattttgca aaaaaaggcg atcattcata acttaaacgt ctttcagtca
     1921 ctccgggatt tcccatcatc gcagactgaa agactctgac tggagcgggc agcgggaatc
     1981 gaacccgcat catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatatg
     2041 gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg c
//
LOCUS       DAJVRL010000228         2068 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVRL010000228 DAJVRL010000000
VERSION     DAJVRL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.237"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..109
                     /locus_tag="OQN02_004018"
     CDS             <1..109
                     /locus_tag="OQN02_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS1644611.1"
                     /translation="LQTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            102..1061
                     /gene="ssuA"
                     /locus_tag="OQN02_004019"
     CDS             102..1061
                     /gene="ssuA"
                     /locus_tag="OQN02_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCS1644612.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1058..>2068
                     /gene="ssuD"
                     /locus_tag="OQN02_004020"
     CDS             1058..>2068
                     /gene="ssuD"
                     /locus_tag="OQN02_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCS1644613.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEE"
BASE COUNT          469 a          566 c          569 g          464 t
ORIGIN      
        1 tctgcaaacc catcttgata ccgcgctaga aactttctgg caggcattgc accgccgcga
       61 tgttcaggtt cctgaccttc tgtctctgcg aggtaatgcc catgcgtaac atcattaaac
      121 tggcgctggc gggattgctt agcgtctcta cttttgcggt tgctgcagaa tcctcgcctg
      181 aagcgttacg tataggctat cagaaaggca gtattggtat ggtgctggca aaaagccacc
      241 agttactgga aaaacgctat ccgcaatcaa aaatctcctg ggtggagttc cccgcgggtc
      301 cgcaaatgtt ggaagcgtta aacgttggca gtattgatct cggcagtacc ggggatattc
      361 cgccaatctt tgcccaggct gccggggctg atttggtata tgtgggcgtc gagccaccga
      421 agcccaaagc cgaagtgatt ctggtggcag aaaacagccc gatcaaaacc gtagccgatc
      481 ttaaaggtca caaagttgcc tttcagaaag gttccagttc acacaacctt ttactgcgtg
      541 cactgcgtca ggccggactt aaattcaccg atatccagcc cacttacctg acgcctgccg
      601 atgcccgcgc cgcgttccag caaggtaacg ttgacgcctg ggctatctgg gatccctact
      661 actccgctgc gttattacag ggcggcgtgc gggtgctgaa agacggcacc gatctcaatc
      721 aaactggatc gttttatctg gcagctcgtc cctatgcaga aaaaaacggc gcttttattc
      781 agggcgtact ggcaaccttt agtgaggccg atgcgttaac ccgcagccag cgcgaacaaa
      841 gcatcgcttt actggcaaaa acgatgggct taccggcacc ggtgattgcc tcttacttag
      901 atcatcgccc tcctaccacc atcaaaccgg ttaacgccga ggttgccgcc ttacagcagc
      961 aaacggcaga tctgttttat gaaaaccgtc tggtgccgaa aaaagtcgat attcgccagc
     1021 gcatctggca gcccactcaa ctggaaggaa aacaattatg agtctgaata tgttctggtt
     1081 tttaccgacc cacggtgacg ggcactatct gggaacggaa gaaggttcac gcccggttga
     1141 tcacggttat ctgcaacaaa ttgcgcaagc ggcggatcgt ctgggctata ccggtgtgct
     1201 gatcccaacg gggcgatcgt gtgaagatgc gtggctggtt gcagcgtcga tgatcccggt
     1261 gacgcagcgg ctgaagtttc ttgtcgccct gcgtcccagc gtaacctcac ctactgttgc
     1321 cgcccgccag gccgccacgc ttgaccgtct ctcaaatggt cgtgcgttgt ttaatctggt
     1381 cacaggcagc gatccacaag agctggcagg cgacggcgtg ttccttgatc atagcgagcg
     1441 ctacgaagcc tcggcggaat ttacccaggt ctggcggcgc ttattgctgg gggaaaccgt
     1501 caatttcaac ggtaaacata ttcatgtgcg cggagcaaaa ctgctcttcc cgccgattca
     1561 acagccttat cctccgcttt actttggcgg atcgtcagat gtcgcccagg agctggcggc
     1621 agaacaagtt gatctctacc tcacctgggg cgaaccgccg gaacttgtta aagagaaaat
     1681 cgaacaagtg cgggcgaaag ctgccgcgta tgggcgcaaa attcgtttcg gtattcgtct
     1741 gcatgtgatt gttcgtgaaa ctaacgacga agcgtggcag gccgccgagc ggttaatctc
     1801 gcatcttgat gatgagacta tcgccaaagc acaagccgca ttcgcccgga cggattccgt
     1861 aggacaacag cgaatggcgg cgctacataa cggcaagcgc gacaatctgg agatcagccc
     1921 caatttatgg gcgggcgttg gcttagtgcg cggcggtgcc ggaacggcgc tggtgggcga
     1981 tggtcctacg gtcgccgcgc gaatcaacga atacgccgcg cttggcatcg acagctttgt
     2041 gctttcgggc tacccgcatc tggaagaa
//
LOCUS       DAJVRL010000229         2040 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVRL010000229 DAJVRL010000000
VERSION     DAJVRL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.238"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..400
                     /locus_tag="OQN02_004021"
     CDS             <1..400
                     /locus_tag="OQN02_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1644614.1"
                     /translation="GSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEIT
                     VDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDALRR
                     IAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            412..1665
                     /gene="proA"
                     /locus_tag="OQN02_004022"
     CDS             412..1665
                     /gene="proA"
                     /locus_tag="OQN02_004022"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1644615.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            1780..1855
                     /locus_tag="OQN02_004023"
     tRNA            1780..1855
                     /locus_tag="OQN02_004023"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1813..1815,aa:Thr,seq:cgt)
     gene            complement(1870..>2040)
                     /locus_tag="OQN02_004024"
     CDS             complement(1870..>2040)
                     /locus_tag="OQN02_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1644616.1"
                     /translation="SVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNL
                     SQSKNKEGTNDV"
BASE COUNT          494 a          506 c          576 g          464 t
ORIGIN      
        1 gggcagcaag ccgggcgtta ttggtgatgt gatggaaggc atttccgtcg gtacgctgtt
       61 ccatgcccag gcgactccgc ttgaaaaccg taaacgctgg attttcggtg cgccgccggc
      121 gggtgaaatc acggtagatg aaggggcaac tgccgccatt ctggaacgcg gcagctccct
      181 gttgccgaaa ggcattaaaa gcgtgactgg caatttctcg cgtggtgaag tcatccgcat
      241 ttgcaacctc gaaggtcgcg atatcgccca cggtgtcagt cgttacaaca gcgatgcatt
      301 acgccgtatt gccggacacc actcgcaaga aattgatgca atactgggat atgaatacgg
      361 cccggttgcc gttcaccgtg atgacatgat tacccgttaa ggagctggcc gatgctggaa
      421 caaatgggca ttgccgcgaa gcaagcctcg tataaattag cgcaactctc cagccgcgaa
      481 aaaaatcgcg tgctggaaaa aatcgccgat gaactggaag cacaaagcga aatcatcctc
      541 aacgctaacg cccaggatgt tgctgacgcg cgagccaatg gccttagcga agcgatgctt
      601 gaccgtctgg cactgacgcc cgcacggctg aaaggcattg ccgacgatgt acgtcaggtg
      661 tgcaacctcg ccgatccggt ggggcaggta atcgatggcg gcgtactgga cagcggcctg
      721 cgtcttgagc gtcgtcgcgt accgctgggg gttattggcg tgatttatga agcgcgcccg
      781 aacgtgacgg ttgatgtcgc ttcgctgtgc ctgaaaaccg gtaatgcggt gatcctgcgt
      841 ggtggtaaag aaacctgtcg cactaacgcg gcaacggtgg cggtgattca ggacgccctg
      901 aaatcctgtg gcttaccggc gggtgccgtg caggcgattg ataatcctga ccgtgcgctg
      961 gtcagtgaaa tgctgcgtat ggataaatac atcgacatgc tgatcccgcg tggtggcgct
     1021 ggtttgcata aactgtgccg tgaacagtcg acaatcccgg tgatcacagg tggtataggc
     1081 gtatgccata tttacgttga tgaaagtgca gagatcgctg aagcattaaa agtgatcgtc
     1141 aacgcgaaaa ctcagcgtcc gagcacatgt aatacggttg aaacgttgct ggtgaataaa
     1201 aacatcgccg atagcttcct gcccgcatta agcaaacaaa tggcggaaag cggcgtgaca
     1261 ttacacgcag atgcagctgc gctgacacag ttgcaggcag gccctgcgaa ggtggtggcg
     1321 gttaaagccg aagagtatga cgatgagttt ctgtcattag atttgaacgt caaaatcgtc
     1381 agcgatcttg acgatgccat cgcccatatt cgtgaacacg gcacacaaca ctccgatgcg
     1441 atcctgaccc gcgatatgcg caacgcccag cgttttgtta acgaagtgga ttcgtccgct
     1501 gtttacgtta acgcctctac gcgttttacc gacggcggcc agtttggtct gggtgcggaa
     1561 gtggcggtaa gcacacaaaa actccacgcg cgtggcccaa tggggctgga agcactgacc
     1621 acttacaagt ggatcggcat tggtgattac accattcgtg cgtaaataaa accgggtgat
     1681 gcaaaagtag ccatttgatt cacaaggcca ttgacgcatc gcccggttag ttttaacctt
     1741 gtccaccgtg attcacgttc gtgaacatgt cctttcaggg ccgatatagc tcagttggta
     1801 gagcagcgca ttcgtaatgc gaaggtcgta ggttcgactc ctattatcgg caccattaaa
     1861 atcaataagt tacacatcat tagtaccttc cttatttttt gactgggaca aatttgggac
     1921 cgatgggttc aggatcgagt ctatttgccg tgcgtgttcg gtaaggtgat taggtgcaag
     1981 gtgagcatat cgacgaacca tttcgataga ctcccagcct cccatttcct gtaacactga
//
LOCUS       DAJVRL010000230         2035 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVRL010000230 DAJVRL010000000
VERSION     DAJVRL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.239"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>2035
                     /gene="sat"
                     /locus_tag="OQN02_004025"
     CDS             <1..>2035
                     /gene="sat"
                     /locus_tag="OQN02_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCS1644617.1"
                     /translation="SYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHG
                     IASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQ
                     EKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKES
                     IVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGK
                     VILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVL
                     AINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQG
                     HATEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDW
                     KTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGF
                     DFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVI
                     AQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASV
                     TGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNN
                     AIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          746 a          359 c          401 g          529 t
ORIGIN      
        1 tcatacggca tgatgattta taaaaatgat aaaacatttc gtaatcttga aatattcgga
       61 gacagcggct ctggagcata cttatatgat aacaaactag aaaaatgggt attagtcgga
      121 acaacccatg gtattgccag cgttaatggt gaccaactga catggataac aaaatacaat
      181 gataaactgg ttagtgagtt aaaagatacc tatagtcata aaataaatct gaatggcaat
      241 aatgtaacca ttaaaaacac agatataaca ttacaccaaa acaatgcaga taccactggt
      301 actcaagaaa aaataactaa agacaaagat attgtgttca caaatggggg aaatgttctg
      361 tttaaggata atttggattt tggtagcggt ggtattatct ttgacgaagg ccatgaatat
      421 aacataaacg gtcagggatt tacatttaaa ggagcaggaa ttgatatcgg aaaagaaagc
      481 attgtaaact ggaatgcatt gtattccagt gatgatgttt tacacaaaat aggccccggt
      541 actctgaatg ttcaaaaaaa acagggggca aatataaaga taggtgaagg aaatgttatt
      601 cttaatgaag aaggaacatt taacaatata taccttgcaa gcggaaatgg taaggtaata
      661 ctaaataaag ataattccct tggcaatgat caatatgcgg ggatattttt tactaaacgt
      721 ggtggcacgc tagatttaaa tggacacaat cagactttta ctagaattgc cgccactgac
      781 gatggaacaa caataactaa ctcagataca acgaaagaag ccgttctggc aatcaataac
      841 gaagactcct acatatatca tgggaacata aatggcaata taaaactaac gcacaatatt
      901 aattctcagg ataagaaaac taatgcaaaa ttaattctgg atggtagtgt caacacaaaa
      961 aatgatgttg aagtcagtaa tgccagtctt accatgcaag gccatgcaac agagcatgca
     1021 atattcagaa gcacagcgag tcattgctcc ctggtatttc tttgtggaac ggactgggtc
     1081 accgttttga aagaaacaga gagttcatat aataaaaagt tcaattctga ttacaaaagt
     1141 aataatcagc agacctcatt tgatcagcct gactggaaaa ccggggtgtt taaatttgat
     1201 acattacacc tgaacaatgc tgacttttca atatcacgca atgccaatgt tgaaggaaat
     1261 atatcagcaa ataaatcagc tatcacaatc ggcgataaaa atgcttacat tgataatctt
     1321 gcagggaaaa atattactaa taatggtttt gacttcaaac aaactatcag tactaatcta
     1381 tccataggag aaactaaatt tacaggtggc atcactgcac ataacagcca aatagccata
     1441 ggtgatcaag ctgtagttac acttaatggt gcaacctttc tgaataatac tcctataagt
     1501 atagataaag gagcaaaagt tatagcacaa aattccatgt tcacaacaaa aggtattgat
     1561 atctccggtg aactgactat gatgggaatc cctgaacaga atagtaaaac tgtaacgccg
     1621 ggtctccact acgctgctga tggattcagg ctgagtggtg gaaatgcaaa tttcattgcc
     1681 agaaatatgg catctgtcac cggaaatatt tatgctgatg atgcagcaac cattactctg
     1741 ggacagcctg aaactgaaac accgactata tcgtctgctt atcaggcatg ggcagagact
     1801 cttttgtatg gctttgatac cgcctatcga ggcgcaataa cagcccccaa agctacagtt
     1861 agcatgaata atgcgatctg gcatctaaat agccagtcat caattaatcg tctagaaaca
     1921 aaagacagta tggtgcgttt tactggtgac aatgggaagt ttacaaccct tacagtggac
     1981 aaccttacta tagatgacag tgcatttgtg ctgcgtgcaa atctggccca agcag
//
LOCUS       DAJVRL010000231         2033 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVRL010000231 DAJVRL010000000
VERSION     DAJVRL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.240"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..363)
                     /locus_tag="OQN02_004026"
     CDS             complement(<1..363)
                     /locus_tag="OQN02_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1644618.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="OQN02_004027"
     CDS             complement(430..1644)
                     /locus_tag="OQN02_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCS1644619.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          412 g          494 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaa
//
LOCUS       DAJVRL010000232         1951 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVRL010000232 DAJVRL010000000
VERSION     DAJVRL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.241"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OQN02_004028"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OQN02_004028"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1644620.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>1951
                     /locus_tag="OQN02_004029"
     CDS             1647..>1951
                     /locus_tag="OQN02_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1644621.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          539 a          403 c          493 g          516 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa a
//
LOCUS       DAJVRL010000233         1943 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVRL010000233 DAJVRL010000000
VERSION     DAJVRL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.242"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..698
                     /locus_tag="OQN02_004030"
     CDS             <1..698
                     /locus_tag="OQN02_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644622.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNGN
                     TTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFN
                     GTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPV
                     LDVSDISVKLQEA"
     gene            700..1305
                     /locus_tag="OQN02_004031"
     CDS             700..1305
                     /locus_tag="OQN02_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1644623.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1292..1840
                     /locus_tag="OQN02_004032"
                     /pseudo
     CDS             1292..1840
                     /locus_tag="OQN02_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          476 a          463 c          534 g          470 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtcagtat ccattaacac ccggttcgct ttatgttggc gtgtacggcg
      241 gcagcccggc agatatcgcg gcggccatct ggcgtaaggc tccgccgggt attgatatga
      301 atggcaacac aacgttcact gttgcagata aggagtacga tccgccgtat cctgaatacg
      361 tgatcagctg gcagacactc aaacccgtca gtctgcatgt cagtgtgacg ctgaaaaaaa
      421 gtgactacct gccttcagat attacccgac aggtacagca atccgtgctg gacgcattta
      481 atggtacaga tggtggtctg cgggcaagag ttgcctctgt tgtctctgcc gggcgctact
      541 atgccggcat ttacaaaacc gatccggaac atattgatat tctgggcctt actgtgagcc
      601 gtgatggttc gtcatggaca acggctgtca ctttcgggat agatgagatt ccggttctgg
      661 atgtgtcgga tatcagtgtg aaactccagg aggcataacg tgcagaatgt ggctgccact
      721 gtgcttgcgc agtatgctgc cagcccccga ctcaatgccc tcattaacag ctttaacgca
      781 gcgctttccc ccgacagttt tatcagtgat ttttatggtc ttatctggaa catcgatacc
      841 gcagaaaagt atggtcttga tgtctgggga aagattgtgg gtgtcagttg ctggctgacg
      901 gtaaaggacg attttaatta cctgggcttc agcgagtcca ggatggacac cccggtaatg
      961 gatgatccct gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact
     1021 gttgacctgt ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt
     1081 actgactgtt ccgttccgga tattaacctg atgctgagat ttatgttcgg aaaaaaacgc
     1141 cgggcttatg ttctgaataa tggcgggctg aggatgagtt acgtctttga gtccgcgctc
     1201 tcgttggcag aactggcgat tatccagtcg tcgggtgcac tgccatcccc gccgggtgtt
     1261 tatgtttcag tagttttaaa ggagtcccgt aatgaaggcc agtgataaac cccgccagct
     1321 ggcggtcccc tttgcgagta ccggagataa aaaccgtatc ccggacaagg cgacacagca
     1381 gaccagagag agcggtaatg ctgcgtatga ttcaggtttt cctccggtga ccatgacagc
     1441 ggtctcagcg ggaggtatac cgccacacgg caaggatttt aacggtctga tgtacgatat
     1501 taccgcagca atacggttcg cccaggctgg cggtttgtac acgtataatg ccggttttgc
     1561 gggggccatt ggtggatatg caaaaggagc cattctcgcc ggagtcgcaa caacagcggt
     1621 ctggctgaat accacggacg ataacctgac cgatcctgaa ggctccgaca gtgcgggctg
     1681 ggtaaatctt cttgaggatc cgaaaaggat attcctgcgg cagaagaaca atctgtcaga
     1741 ccttcagaat aaagggacgg cacgggataa tcttcaggtt tacagtaaag agcagtcaga
     1801 tcaacgctat gttcatcggg aaggcgataa aataaccgga gagctgaaaa tccgtggtgt
     1861 taatgcgctg aggattttca acgaagcttt tggcctgatt tttcgtcgtt cggaagagtg
     1921 cctgcacctt attcccacca gtg
//
LOCUS       DAJVRL010000234         1936 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVRL010000234 DAJVRL010000000
VERSION     DAJVRL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.243"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..545)
                     /locus_tag="OQN02_004033"
     CDS             complement(<1..545)
                     /locus_tag="OQN02_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644624.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1200..>1936)
                     /locus_tag="OQN02_004034"
     CDS             complement(1200..>1936)
                     /locus_tag="OQN02_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644625.1"
                     /translation="NGAFNPAKDAPSTTVIKGASVTVTPVLNPETNKTGILCSAATHV
                     SDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVVDVLKDTSDLQIIEAAPLSGKPSDQ
                     AEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAILDRAAQRAGHEGISELLGCTVCGY
                     AGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVVDVLATGSVK
                     VKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          544 a          441 c          417 g          534 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg ggttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggaacctagg tatcgagggt atcgaagata atcagccgct aaccatttgt ttccttaaag
      841 gatgatgaat ccttgtggtt acagttatgg ttacacatta atttatctct ggtcaagtcc
      901 cgaagtccta actttaagac agtggcccca atcgtccaga cttgaaaggg gcctatgggg
      961 gaagtcgctg acctttatct attaaatacc tacagagatt tttgtgagat tttttaatcc
     1021 aaggtcataa cctactgccc ccacaggccg caaagtgaag acctgtaagc accagagcaa
     1081 tagcgagtat ctatcactcc ctgtagagta tctggtagac caagaggaca cagaggacga
     1141 ccatatacaa ctcctgtaag ctcatggatg tactccttat gttcactcag ggtttatagt
     1201 tactctgcgg cagtaaaacg aataacatgg accaacggga aagaggcgtc ttcctcaacg
     1261 tcaaactcac ctgctttcac cttaacggaa ccagtagcca acacatcgac cacactgatt
     1321 aactcaaggt catacccagc agtgttgcta tttcgggtca ctttgacatc taccgtctta
     1381 gcgtctttag ttgacctgaa agagatagaa gcgaaccgct taggcaggag atagacacct
     1441 gtatcatcgc ccgcatagcc gcatacagtg cagcccaata gctcactaat accttcatga
     1501 cctgcgcgtt gagcggctcg gtccaagatt gcctcaagtt tctcagggac caatacagcg
     1561 taatcgctca gtgtatttcc caactcgacc ggaagattca acgctaatgt atccagaaga
     1621 tcttcagctt gatcagacgg tttaccactt agtggggctg cctcaatgat ttgaaggtct
     1681 gacgtatctt tcaggacatc gaccacagcg gcgaacatct ggcggcagta ttgaacctgc
     1741 atatcgcata cagtctggat taatgccccc tcggtccaac cgcccatcat atcgaggtca
     1801 gacacatgag tagcagcaga acacaggatg cccgtcttat tggtctcagg gttcaacact
     1861 ggggtgacag ttacgcttgc ccctttgatg actgtggtag acggagcatc cttagctgga
     1921 ttaaacgcgc cattat
//
LOCUS       DAJVRL010000235         1936 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVRL010000235 DAJVRL010000000
VERSION     DAJVRL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.244"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OQN02_004035"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OQN02_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCS1644626.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1936
                     /locus_tag="OQN02_004036"
     CDS             1494..>1936
                     /locus_tag="OQN02_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCS1644627.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          552 a          337 c          466 g          581 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatga
//
LOCUS       DAJVRL010000236         1913 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVRL010000236 DAJVRL010000000
VERSION     DAJVRL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.245"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(53..1702)
                     /locus_tag="OQN02_004037"
     CDS             complement(53..1702)
                     /locus_tag="OQN02_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1644628.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAJVRL010000237         1880 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVRL010000237 DAJVRL010000000
VERSION     DAJVRL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.246"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..160)
                     /locus_tag="OQN02_004038"
     CDS             complement(<1..160)
                     /locus_tag="OQN02_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1644629.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATE"
     gene            217..885
                     /locus_tag="OQN02_004039"
     CDS             217..885
                     /locus_tag="OQN02_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1644630.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1201..>1880
                     /locus_tag="OQN02_004040"
     CDS             1201..>1880
                     /locus_tag="OQN02_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1644631.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          583 a          399 c          348 g          550 t
ORIGIN      
        1 cctccgttgc gatctccaga tctacagcat cctgaagtgg cgcaatatgc tcactgaatc
       61 cctggatgta gaactgtgtg gtgacggtct tccagccatt cggctcctgc tgtatcgaag
      121 cataccaggc tatttcaata tcgctatgct gcggcagcat ttaacccctt gtaattcatc
      181 gccataattg atttaattca caaataaaac tataatatgg tgaaattaat gaaaaaaaac
      241 acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga ctggtgggtt
      301 ttgggagtat caaatcggct tgcatgggga tgtcctacaa aggaacacct tcttccacac
      361 tttctggaac atttaggtaa caaccatctg gatattggtg ttggaactgg gttttacctt
      421 actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc tagcctgaac
      481 gcggcatcta caagggctgg ggaatcaaaa attaaacata aaattagcca tgatgttttt
      541 gaaccttatc ctgcggcgtt acatggtcaa tttgattcca tttccatgtt ttaccttctt
      601 cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc ggcgcaggcc
      661 ttaactgatg atggaactct atacggagcc acaattcttg gcgatggagt tgtgcacaat
      721 agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc aaacacaaaa
      781 gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt taaaaccaag
      841 gttcaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc aaccgcagca
      901 cgttcttgct taagacgtgc tacggtttcc tttacgattt ggggttgcga ctttacctct
      961 attgataatg cattccggca gaacgttcaa atatgaacgt acgatattta actaaccgaa
     1021 aaacaaaata tagcacaggc gagacattta ccatctaaga aaatagtatc gtttttttac
     1081 agttaaatca acatcacttc cttaaaatga aaacaataaa tttcaatcct gaaacatttt
     1141 atttcagctt attattcccc agtatcacat tttatcagga tatccttctg cgaggttatt
     1201 atgcttcctg taaataatcc ccccctatcc actggaaacg tctcttttta cagaactaca
     1261 tcaatcgaca atgttcacaa taattatctc tccgaatggg ttgaatggac taaaaacagc
     1321 atttccggag aaaacaggga aactgctttt acccggctcc aattatgtct ggagaacagt
     1381 gaaacatcgt tggacttatc ttgtttaggt ctcagatctc taccacgatt gcctgacaat
     1441 cttgatgaaa ttaatgtaag caataaccaa ctatcaatgc tccccgagct accaagggca
     1501 ttgaaagagc tgaatgcaag cagtaatcaa ttatctgcac ttcctgaatt accagtgtcg
     1561 ctggaatata taaatgtgag tgataaccat ttgttcgcac ttcctgaatt acctgcgtca
     1621 ctagaatata ttaatgtaag tgacaatcac ctgtctgtac ttccgaggtt accaatgtca
     1681 ttggaattac ttgatgcagc cagaaatgct ttggaagtaa taccagattt tccagaaaga
     1741 gatgatcata ttataagaat attctggctt aatcagaacc ggatcacggc aattccggaa
     1801 agcatacttg gcctcagttc tgatagcgtt gtcaatctta gagaaaatca actatctccc
     1861 agaataatgc aaactttgtt
//
LOCUS       DAJVRL010000238         1863 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVRL010000238 DAJVRL010000000
VERSION     DAJVRL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.247"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            57..197
                     /locus_tag="OQN02_004041"
     CDS             57..197
                     /locus_tag="OQN02_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644632.1"
                     /translation="MKYGITLLMLCLLAGCAQRNTQLPPVSGNPEPVNSPAIIQELTP
                     HV"
     gene            190..876
                     /locus_tag="OQN02_004042"
     CDS             190..876
                     /locus_tag="OQN02_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCS1644633.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            869..1762
                     /gene="virB9"
                     /locus_tag="OQN02_004043"
     CDS             869..1762
                     /gene="virB9"
                     /locus_tag="OQN02_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HCS1644634.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPVKQPVTDADGKTVQ
                     KIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNLAFVVRFSYPDDTRRQNDAA
                     RLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAGSESITPDFAYDDGRFTYLG
                     FSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSLSPRFVLRYGSQVVGIENGS
                     YGKVTVANGSTVSPDVKLESK"
BASE COUNT          423 a          578 c          544 g          318 t
ORIGIN      
        1 ggacgccgca acggcgtcct gcggcttcgt cacgtcttta ccacaggaaa aacaccatga
       61 aatacggcat cacgctgctg atgctgtgcc tgcttgcagg ctgcgcacag cgaaacaccc
      121 aacttccgcc cgtgtcgggc aacccggagc cggtcaattc cccggccatt attcaggagt
      181 taacccccca tgtctgaact taccgccaaa gccctgagcg actcgcgctc gtttgagaac
      241 gccattcttg agcgggatat gcgcatgaaa aaggcgggct ggatcgtggc ggcgctcgcc
      301 ggtctgctgc tcgtcttcgc ccttgccgcc cttatcctgc tgctaccgct caaaaccacc
      361 accgtggagc tgtacacgct cgaccgtcag accgggcgca tcgagcgcgt caccaccgtc
      421 ggcgaggaca agctggacac ctccgaggtg ctcaatctct cgcagactgc gtcctacgtg
      481 aagcgccgcg aggggtacaa ctatttcgcc cttcagcagg actacagaga gacgcagatg
      541 ttcaacagcg acgaggtcaa caaggaatac ctcgactggt ttaacagccc ggacgcgccg
      601 gacgctgtgt ttcagcaggc ggcctatgta gtcacggtgg aagtcctctc caacgtaccg
      661 tcagagggca ccggctccag catggtggag atgctgcgca tcaagcgcac cattcgaaag
      721 gtcaaggaca acaccgaaac gtatgattac tggacggtgc gcatgaccta ccgctatttc
      781 ccgcagaaga aaatgacggc ctccgagcgc gaggtgaacc cgttcggctt catcgtcacc
      841 agttaccagc ggtttaagga gaaaagcgat gaataaggta ctgatgtgtg ccctcctgct
      901 gaccgcagcg tcagcatggg gggcggcgac accgcgcggc agcgcctatg acagccgcat
      961 gcagaacatc ccctacaaca gccagaacac gacagtgatt aacacgcggg cgggttacat
     1021 cacgacgctg gtttttgacg ccgacgagga ggtgatcacc gccaccgctg gttttccgca
     1081 gggctggacg atcacacccg accgtaaccg cgtggggatt agccccgcgc cggtgaagca
     1141 gccggtaacg gatgcggacg gcaaaaccgt ccagaagatt ttcaccccca cgcagcagga
     1201 ctggcagacc aacctgtttg tgaccaccac aaagcgcgac tacagcctga tgctgaacgt
     1261 gatcgataac gatcgcgagg cgggaaatct ggcctttgtg gtgcgcttca gctaccccga
     1321 cgatacccgc aggcagaacg acgcagcccg cctcgcccgt cagcaggagc ttcaggccgc
     1381 gcaggagaaa gaacgcattc agtttgccct gaagaaatcc actgcgccgc gcaactggaa
     1441 ctacacccgc agggtggcgg caggttcgga gtcgatcacc ccggattttg cgtatgacga
     1501 cggccggttt acctatctcg gcttttcacc gcagaagctg atcccgtcgc cgaccgtcat
     1561 cgtcggcggc agcgagcagg tgatcacccc gaccttcagc accgagggta actaccgggt
     1621 gatggtactg cgctctcttt ctccacgctt tgtgctgcgc tacggcagcc aggtcgtggg
     1681 gattgaaaac ggcagttacg ggaaagtcac cgtggcgaac ggcagcaccg tctcaccgga
     1741 tgtgaaactg gagagcaaat gatgaacgaa caggaagaca ttaaaaccgc cgccgaactg
     1801 gaggcggaag cccgcgagcg ggcgcgggca gagattgacg gcgataaacc cgccgcaggc
     1861 gcg
//
LOCUS       DAJVRL010000239         1827 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVRL010000239 DAJVRL010000000
VERSION     DAJVRL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.248"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..122)
                     /locus_tag="OQN02_004044"
     CDS             complement(<1..122)
                     /locus_tag="OQN02_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1644635.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKR"
     gene            complement(161..448)
                     /locus_tag="OQN02_004045"
     CDS             complement(161..448)
                     /locus_tag="OQN02_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644636.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(698..853)
                     /gene="hokD"
                     /locus_tag="OQN02_004046"
     CDS             complement(698..853)
                     /gene="hokD"
                     /locus_tag="OQN02_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1644637.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1081..1746)
                     /locus_tag="OQN02_004047"
     CDS             complement(1081..1746)
                     /locus_tag="OQN02_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1644638.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          473 a          465 c          406 g          483 t
ORIGIN      
        1 cgcttaaagt ctgcgtgtat ccactcacct attttgattt gatgcagaaa atcgcaactc
       61 tccggcgtcg ccgggagaag taatccggaa gaggtttgtt tgaccagttg tatatgtgcc
      121 atcggctttc tccggtggca cggtgttaca cagcaggagt tcaatcctgc tcaagattgt
      181 agatgagttt attcttcagc aaaagcagaa aaccagcctt aaaaccaatc tttttcaaaa
      241 cccgtaatga tgtgacaaat tcgtcctcac gcaaaataaa accgtccgtc agaagtccat
      301 tacaaaaata aaataacaca gcaccgctct tcctttgttg agattgcaaa catctaatgc
      361 ggcaatggct gacaatcgct ccattctcaa cgcgcacagc atagaggcca ttttcactaa
      421 aaatttcacg caattcttcg attttcatct tcagaatcct tccagataaa tagctctccc
      481 ctgttcgggg tccatccctc ttctccctgc gcgctactta agtgagtcga ttctatctgc
      541 gaaggtgcgc gaatcaaatt caccggaaat aaacaacaaa aaacccgccg aaacgctgag
      601 agatcacagc gatgggcggg ttaagtgcgg gtgcgttgag gatgcctgac acatcagagg
      661 tgggcgggga tgggatcagc tccccacccg gtcactctta cttcctggat tcgtagtcta
      721 cgaagacagc gacctccgtc tggccggttc ggattcgtac ctcacagagg tctttcctcg
      781 ttaccagtgc cgtcactatg acggttaaac agatgacgat cagggcgatt aacatcgcct
      841 tttgctgctt catagcctgc ttctccttgc ctttcggcat gtaagaggct aacctacatg
      901 tgcaaagcat gaaattggcc tcagattaat gttaagcgtc ttgccggacg cgtaatgtta
      961 actggggctt ttctctatct gccttttggt gttcatgcct gaagcagata gcctcaagca
     1021 cccgcagtca ttctacttac ctcgccaata tgaaatcaat cagaaaggtg ccccataaaa
     1081 tcactccttc tcttctttac cgtagtggag ttgaccaatt ttgataagag ggcgtccctg
     1141 agatttgcgg tgtagattgg tatcgcgcag agaatacaca cagccacaat attcctgctg
     1201 atagaatttt tcgcgcttgc tgatttcaat catacgggac gagccgccct gcttgcgcca
     1261 gttataatcc cagtacacca tgcccggata atgcgcgacg gctcgccgcc cacagtcgtt
     1321 aacctgctgc atatttttcc agcgtgaaat gcccagtgaa ctgctgatca cactgaaacc
     1381 attttcagcg gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca tggtgcaacg
     1441 gatccctcgt tcgggctccc attccattcc tttggctcgt tcaaaccagt tgtctgtgtc
     1501 gtaatcagca tcaataaacg gcacgccgtg ttgttcagca aagcgaatat tctcatcctt
     1561 acgaattaaa tactctttct gaggatgaat gttcgggttg tagaaaaaga tggtgtagtc
     1621 gattcccgag gcctgaagcg cctccatcac ctcaccggaa catggagcac agcaagagtg
     1681 cagtagtagt ttgtttgccc cgtttgggag ctccaatttg ggccgtttga aatcagcaac
     1741 tgtcataaat atgtttattg aggtcataaa aatatcaaag agtgtagcat tagaacgggg
     1801 ctatcggaaa cagatgtgtg actgctc
//
LOCUS       DAJVRL010000240         1805 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVRL010000240 DAJVRL010000000
VERSION     DAJVRL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.249"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            173..1198
                     /locus_tag="OQN02_004048"
     CDS             173..1198
                     /locus_tag="OQN02_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1644639.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1805
                     /locus_tag="OQN02_004049"
                     /pseudo
     CDS             1266..1805
                     /locus_tag="OQN02_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          461 g          503 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgc
//
LOCUS       DAJVRL010000241         1765 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVRL010000241 DAJVRL010000000
VERSION     DAJVRL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.250"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..285
                     /locus_tag="OQN02_004050"
     CDS             <1..285
                     /locus_tag="OQN02_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1644640.1"
                     /translation="SQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEA
                     TNVIAPLQDAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(576..1577)
                     /locus_tag="OQN02_004051"
     CDS             complement(576..1577)
                     /locus_tag="OQN02_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1644641.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
     gene            1695..>1765
                     /locus_tag="OQN02_004052"
     CDS             1695..>1765
                     /locus_tag="OQN02_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1644642.1"
                     /translation="MASVSISCPSCSATDGVVRNGKST"
BASE COUNT          571 a          352 c          355 g          487 t
ORIGIN      
        1 tcacagagtg taacaaaaat gattacctgt ggtgagaacg gtcaccctgt cattgttgac
       61 cgtccttctc caacaccaga acaattagcc ttaataaatg atgaaaagaa atctgcactg
      121 atagcagagg caacgaatgt aatagcgccg cttcaggatg cggttgattt aggtatggca
      181 acagatgatg aaacgaaact gttactggca tggaaaaaat atcgcgtact gcttatgcgt
      241 gttaatgtag taaaacccga gtggcctatg catccaaata aatagacttg ttaatctagg
      301 gtaattgttg gaataatacg catttttcaa caggtccaga taacctggat ataatgctca
      361 cttctacaat ggcctcaact tattttattt aattgatatt acggaagtta actcatacat
      421 aaacaatatg actcagtact gtactaaata gctgcgtgga aaagaactcc accttcgctc
      481 gtccttgcag gacgaggcat cagaacggtg tccactgcca ggcagtgtta tatctggctt
      541 tgatttattc aggggctcga tggccccttt attattcaat tcagggataa ttttggcgag
      601 cttcgtgtaa ggaatctttt ttcaattaga ttccaagaaa gatgagccaa gaaaagaacg
      661 acaatggcac ttaacaacat cgaagggtaa aagccaatat gcaatgtatt tataataacc
      721 tgctgaacgg ggaatgcata aatgtataca ccataagagt aatcaaacct acccttaacc
      781 agtggatctc caaaagatgt gcatatagct attgttgaga aagaaaccag aatatagcaa
      841 gccattgtat agtcaatgcc tttgccatag ctcgcgtatg cgtacatagc aagcaaagaa
      901 atcacggaaa ttttcacatt acttacattc caagatttct catacatcgc catagtggca
      961 ccaaaaaaga aagccatccc ccgcaaagga tacagccata ctggaatgga gaatattaca
     1021 tccttgttct cagatacact ttcaactaat gaaagtgaaa caaaaatcaa tagtataact
     1081 atgaacgttt ttccgctctt tagtagagca acagccactc cagttattat gtaacacata
     1141 aactccagtg gtagtgtcca caagctgccg ttaataccgg gatgaattaa atgtgatgta
     1201 atgtcggcat ctggagcctg actcattaaa atagagctta tggtcttccg gataatgtca
     1261 tgactaaaat attctgtact gaagttgtta agcatccacc caaataaaaa atacgtcagt
     1321 acagagcaag ggacaagagc agggaatatt cttcttgctc ttttagccat gaaatcaata
     1381 aaggaatcac ttctgattgc tgattttgaa atcaggtatc ctgatatgga aaagaatatg
     1441 ataacagcaa tgccgccagc actttcaaag ccgaatatat acggctcgag ttgtccagag
     1501 agcgcgtagt ggtgactgat tagaaccatc atagctgcca caagccttgc cgtatcaaag
     1561 cagttgctct tatgcattaa aataaaatcc ctaagttttt gtgaggaaaa tatcagatct
     1621 ttatgtcata gtccatttcg gtgatgctgc caacttactg atttagtgta tgatggtgtt
     1681 tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg
     1741 gggtggtgcg taacggcaaa agcac
//
LOCUS       DAJVRL010000242         1728 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVRL010000242 DAJVRL010000000
VERSION     DAJVRL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.252"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            435..1283
                     /locus_tag="OQN02_004053"
     CDS             435..1283
                     /locus_tag="OQN02_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1644643.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1300..1581
                     /locus_tag="OQN02_004054"
     CDS             1300..1581
                     /locus_tag="OQN02_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644644.1"
                     /translation="MNPMLDYCFTSAPQQTITHTGIKNLLWIIKKNLQQKGDPDLIPC
                     AYGLCDRQVSPDKAYTAELIYMHGTMMRKKKRKYCSEICAEKDQMAHEL"
BASE COUNT          492 a          396 c          409 g          431 t
ORIGIN      
        1 ctggcgagat acgttggcat attcatttcc ttactggttg tgtgagaact tcagtaaaga
       61 taccaccaaa gcccggaagt ggtgaaataa aaccgggcac aacacgaagg cgcatttccg
      121 gtatccataa agagtcggtc ttgtctgtga aatttaaatg gtgggagtgc gcttccggtt
      181 gtaaataacg acattgctgt gtgaagtacc agttggcggc atcggtttaa ttgctggctg
      241 atgtccgccc tttttaaagt gaattttgtg atgcggtgaa tgcggctatg cgcacgcggc
      301 acagttaaaa gtcatgttag tccttattgg tttgggtggg aaagccaact gtaattgtta
      361 actggttaac gtcacctgga ggcaccaggc accgcatcaa caaagttcac ttcggtgatg
      421 aaaggaaaga gaaaatgttg aatgtagcta ttgaaaacca gaacgggtgg aattatagtg
      481 cacctgcacc tcataaaacg ggtgccggga ttgctacccc gacgatgact actgcgcata
      541 accgcgctca ggcggttttt ttatgcgtca agcacagcca cattcagatt atggtggggc
      601 gtgcagggca gccgcaaggc tggccggttt cggtagtcac cggttgtagc aaccctgtgc
      661 gtctcaccac ccatgagatt gctacctctg gtggtgagtc atttaaactg actatcgagg
      721 ctgccatcat ggctactatc cttacccttt ctcaccctga cgcaaccatc gaaaatggtc
      781 gcgctgtcac tacgtctgtt gcggttgcgg agttcttccg gaaaatgcac aaaaacgtta
      841 ttcagaagat cgaaactctc gaatgctcgc cggagtttaa ccggcttaat tttaagcccg
      901 tcacctatac cgacgccaaa ggcgaaaaac gcccaatgta ccaaatcacc aaaaacggct
      961 tcgttttcct ggtgatgggc ttcactggca aaaaagccgc cgcattcaag gaagcctaca
     1021 ttgctgagtt cgaccgcatg gaggccgaac tgcgccagaa taacacccca cctgctgaca
     1081 agatgattcc gggtgatggt cgcactctgg ttgttcactt cgacaaattc ggcaatgtcg
     1141 aattcaccga aaccgttcct gatggcgcac tggtctgtac cctggagact ttccggtttt
     1201 atctggaaaa acagggatgg actcttgtta accggggcgc aattaaaaat atgactgtgg
     1261 aacaattact ttctttaaaa taaaaatgga gctacggata tgaaccctat gcttgattat
     1321 tgttttacct ctgcacctca gcagaccata acacacaccg gaatcaaaaa tttgttatgg
     1381 ataatcaaaa aaaacctcca gcagaaaggt gatcccgatt taattccctg tgcttatggc
     1441 ctgtgtgaca gacaagtaag ccctgataag gcttacactg ctgaacttat ttacatgcac
     1501 ggaaccatga tgaggaaaaa gaaacgcaaa tattgtagtg aaatctgcgc cgaaaaagac
     1561 cagatggcac acgaacttta attaactgac tattcgaaac tgaatttatg ccagcaatgg
     1621 cagggattcg ctcaacctta attaaggaga aaaacatgaa caccaattat gaagccactg
     1681 ttgcaactac tgataacatt gttcacgagg tttacctgga aggaaagc
//
LOCUS       DAJVRL010000243         1728 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVRL010000243 DAJVRL010000000
VERSION     DAJVRL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.253"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..243
                     /locus_tag="OQN02_004055"
     CDS             <1..243
                     /locus_tag="OQN02_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1644645.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(332..844)
                     /locus_tag="OQN02_004056"
     CDS             complement(332..844)
                     /locus_tag="OQN02_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS1644646.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1039..>1728
                     /locus_tag="OQN02_004057"
     CDS             1039..>1728
                     /locus_tag="OQN02_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644647.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGID"
BASE COUNT          507 a          386 c          438 g          397 t
ORIGIN      
        1 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga
       61 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
      121 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
      181 agcgaccaga cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg
      241 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa
      301 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      361 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      421 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      481 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      541 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      601 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      661 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      721 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      781 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      841 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      901 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      961 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
     1021 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
     1081 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
     1141 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
     1201 ggtacgcaaa aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa
     1261 aaaagtacgc aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt
     1321 accgatcccc cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt
     1381 tatgcccgtc gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc
     1441 gaggacgaat tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg
     1501 tggttggtgt cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat
     1561 atcagcgccg ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc
     1621 acgcttgcga cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat
     1681 aaggtgtctc tggaggctga tcgtcttcgc cgtgatgcag gtattgat
//
LOCUS       DAJVRL010000244         1717 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVRL010000244 DAJVRL010000000
VERSION     DAJVRL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.254"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(110..577)
                     /locus_tag="OQN02_004058"
     CDS             complement(110..577)
                     /locus_tag="OQN02_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1644648.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OQN02_004059"
     CDS             complement(643..882)
                     /locus_tag="OQN02_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCS1644649.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OQN02_004060"
     CDS             complement(1017..1622)
                     /locus_tag="OQN02_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1644650.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          379 c          303 g          486 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tggttttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata ttttttggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAJVRL010000245         1684 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVRL010000245 DAJVRL010000000
VERSION     DAJVRL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.255"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..444
                     /locus_tag="OQN02_004061"
     CDS             <1..444
                     /locus_tag="OQN02_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1644651.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            445..810
                     /locus_tag="OQN02_004062"
     CDS             445..810
                     /locus_tag="OQN02_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1644652.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            807..1490
                     /locus_tag="OQN02_004063"
     CDS             807..1490
                     /locus_tag="OQN02_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1644653.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          478 a          354 c          457 g          395 t
ORIGIN      
        1 ccgggagagc agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg
       61 cctcacgccc accagcaaca gaatccacca caggaagaga cggtggtcag cattgccgtt
      121 gatccggaat ctccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
      181 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
      241 ccgcaccaca tcattggtca tggactggga gggatgggaa caaaggctga cgatttgttt
      301 gttattccgc tgtgtcgtaa atgccataac gaactacacg ccggggtaaa agattttgaa
      361 gaaaaacacg gtagtcagtt gttgttgctg attcgttttt taatgcacgc gagaaattcg
      421 ggtgttctga agtggaaagc ataaatgacc gaacgcatag aatttgtttt gccttacccg
      481 ccaacggtga acacctactg gcgacgtcgt ggcagcacat attttgtatc aaaagccggt
      541 gagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcgactgaa attaaacctg
      601 tccggaaggc tggcgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
      661 gacaatatcc tgaaggcacc gctggatgcg ctgacgcatg ctggactact tatagacgac
      721 gagcagtttg atgaaatcaa tattgtgtgc ggtcagctcg ttcctggtgg gcggctgggg
      781 ataaaaatca cagaactggg gtgcgcatga ataaccagta tttacagttt gtgcgtgagc
      841 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
      901 aagagaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg taccgcgatg
      961 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     1021 cgaaggggac gtcaattcct ctggtcagtc cggttgagtt ttcgacatca tcgtggcggc
     1081 gggctgttct gtctcttgaa gagcactata aagcctggtt gttgtggtgt tacagcggga
     1141 gtatttgttg ggaatatcag atcgcgataa cacagtgggc gtggaatgaa tttaatgctc
     1201 aatccggtac cagaaaaatt gcagggaaaa cgcaggaacg cctgaaaaaa ttaatctggc
     1261 tggcggcgca ggcagtaaaa gcagaacttt ttggtgggga aggttatgaa taccaggagc
     1321 tggcattact ggcgggagtg acaactaaaa actggtccaa aacatttact cgtcactggg
     1381 ttgcaatgaa acacattttt caccgactgg atagtgaggc tttattgttt gtaatgagaa
     1441 cacgttcaaa aaaaagacgg cattttcaaa gcaaagtgtt gcaaaagtag attgaaaggc
     1501 atatattcat gcaaatctga tactttgccg attttgtacg tgatggcaaa agcaaacaaa
     1561 acccgcccac aagcgggttt tttgtgccac ttatctcgga tagacatggt gaatgcgctg
     1621 gtggaggaga taagggtgat ttttgaatgc ttgcaacatt gatttcgtaa cgttattatc
     1681 ctgc
//
LOCUS       DAJVRL010000246         1597 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVRL010000246 DAJVRL010000000
VERSION     DAJVRL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.256"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..636)
                     /locus_tag="OQN02_004064"
     CDS             complement(<1..636)
                     /locus_tag="OQN02_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1644654.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="OQN02_004065"
     CDS             complement(750..1322)
                     /locus_tag="OQN02_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1644655.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          475 a          336 c          317 g          469 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttta ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJVRL010000247         1538 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVRL010000247 DAJVRL010000000
VERSION     DAJVRL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.257"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(910..1386)
                     /locus_tag="OQN02_004066"
     CDS             complement(910..1386)
                     /locus_tag="OQN02_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1644656.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          348 a          410 c          385 g          395 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DAJVRL010000248         1377 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVRL010000248 DAJVRL010000000
VERSION     DAJVRL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.258"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..250
                     /locus_tag="OQN02_004067"
                     /pseudo
     CDS             1..250
                     /locus_tag="OQN02_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            743..>1377
                     /locus_tag="OQN02_004068"
     CDS             743..>1377
                     /locus_tag="OQN02_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1644657.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          338 a          319 c          420 g          300 t
ORIGIN      
        1 caacacaaag gcaactaata aagatacaga taaagatcta aaaacagatc taacccatcc
       61 caaacccttc ccttccggaa gggagtttcg ggattttgtg gctggagtgc ttgaggggag
      121 attatctggc ggtactgcag cggaattttg taattctgcg gtggttgcgt tgcaggctgc
      181 tggcctggat gtctgtcgtg agtatccggt gccagagcgt ggtgacggtt gcggagggcg
      241 gattgatatc gtcgtgactg acaggaacgg tgtccggtgt gggatcgagc ttgaccgaaa
      301 ttctccgcga cagaaatcac tgctcaaaat cggtgctgtt gaaaccggga tatgtgtctt
      361 gcggcgcagt gatatcgcaa ggcacaccga gcagggaatt ctggttatcg gtggggctgt
      421 tcgccagaaa aaatttgacc cgttgtcagt tgatctgccc gactggttgc cagaaacact
      481 ctggcatgag tgggtccagt tcaggcaggc attgcgaaaa ccgattcgaa cggagctggg
      541 cgctaacggg gcgatacggg aactggaaaa attccgtcag cagggtttta cacctgagca
      601 ggtgattcga cacagcatcg ccaatgaata ccagggtctg ttcgcgccga aaggtgttcg
      661 gcctgagacg ttgctccgac aggttaacac cgtctcgttg ccggacagtg cgatcccgct
      721 aggcttcagg gggtaacaga ccatgaaaaa tattgcgaca ggaggcgttc tggagcgtat
      781 ccgcagactg accccaccac atgtaaccgc cccattcaga acggttgcgg agtggcgcga
      841 gtggcaactt gctgaaggcc agaaacgttg cgaggagatc aaccgcctga atcgtcagtt
      901 gcgggtggaa aaaatcctcc gctccggcat ccagccgttg caccggaagt gttcgtttgc
      961 gaattaccgg gcgcagaacg acggtcagcg acatgcactg agtcaggcga aatccatagc
     1021 tgacgaactg atgacaggct gtacgaattt tgtgttcagc ggtaagcccg gcaccggaaa
     1081 aaatcatctt gcagccgcga ttggcaaccg gctgatggcg aaggggcgta gcgtgattat
     1141 cgtcaccgtg tccgatgtca tgagcgtgtt gcatgagagc tacgacaacg gcaaatccgg
     1201 tgaaaaattt ttacaggagc tttgtggtgt tgacctgctg gtcctggatg aaattggcat
     1261 gcagcgggat acgaaaaacg agcaggtggt actgaaccag attgttgatc gccggacggc
     1321 atcgttacgc ggtgtgggga tgctgacaaa tattaaccat gcagcgatga atacact
//
LOCUS       DAJVRL010000249         1363 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVRL010000249 DAJVRL010000000
VERSION     DAJVRL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.259"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(331..594)
                     /gene="rpsT"
                     /locus_tag="OQN02_004069"
     CDS             complement(331..594)
                     /gene="rpsT"
                     /locus_tag="OQN02_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1644658.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            697..915
                     /locus_tag="OQN02_004070"
     CDS             697..915
                     /locus_tag="OQN02_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1644659.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            923..>1363
                     /locus_tag="OQN02_004071"
     CDS             923..>1363
                     /locus_tag="OQN02_004071"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="catalyzes the formation of FMN from riboflavin and
                     the formation of FAD from FMN; in Bacillus the ribC gene
                     has both flavokinase and FAD synthetase activities;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FMN
                     adenylyltransferase"
                     /protein_id="HCS1644660.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEY"
BASE COUNT          326 a          292 c          345 g          400 t
ORIGIN      
        1 aaggtctaat gaggagatat ttatgaatgg ttaaagtata taggaattta attcaaaaat
       61 agagtggtct taagtttaat acctcaatgt gtatcacaat atccatattc tttgtggggg
      121 agtctggaga ttgagtagat attcttgttc agaatgtatc agccgatggt tctacgattc
      181 ttaagccacg aagagttcag atagtacaac ggcatgtctc ttttgactat ctggcaaccg
      241 gcagtgtgtt ctctcacgca tcacaaaagc agcaggcata aaaaaacccg cttgcgcggg
      301 ctttttcaca aagcttcagc aaattggcga ttaagccagt ttgttgatct gtgcagtcag
      361 gttagcctta tgacgtgcag ctttgttttt gtggatcaga cctttagcag cctgacggtc
      421 cacgatcggt tgcatttcgt taaatgcttt ctgtgcagca gctttgtcgc cagcttcgat
      481 agctgcgtat actttcttga tgaaagtacg catcatagag cgacggcttg cgttgtgctt
      541 acgagccttt tcagactgaa tggcgcgctt cttagctgat ttgatattag ccaaggtcca
      601 actcccaaat gtgttctata tggacaattc aaaggccgag gaatatgccc ttttagcctt
      661 cttttgtcaa tggatttgtg caaataagcg ccgttaatgt gccggcactc gttacgtagt
      721 gatggcgcag gattctacca gcttgcgggg tgtgaataca gcttttccgc gataaaaatt
      781 gcagcaggcg gtcagtttct tcccgtgatt tgctccatgg caatgaaaag ccacttcttt
      841 ctgatttcgg tactcaatcg ccggttaacc ttgaccgctg tacaaggtat actcggacga
      901 ttttcactgt tttgagccag acatgaagct gatacgcggc atacataatc tcagccaggc
      961 cccgcaagaa gggtgtgtgc tgactattgg taatttcgac ggcgtgcatc gcggtcatcg
     1021 cgcgctgtta cagggcttgc aggaagaagg gcgcaagcgc aacttaccgg tgatggtgat
     1081 gctttttgaa cctcaaccac tggaactgtt tgctaccgat aaagccccgg caagactgac
     1141 ccggctgcgg gaaaaactgc gttaccttgc agagtgtggc gttgattacg tgctgtgcgt
     1201 gcgtttcgac aggcgttttg cggcgttaac cgcgcaaaat ttcatcagcg atcttttggt
     1261 gaagcatttg cgcgtaaaat ttcttgccgt aggtgatgat ttccgctttg gcgctggtcg
     1321 tgaaggcgat ttcttgttat tacagaaagc tggcatggaa tac
//
LOCUS       DAJVRL010000250         1324 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVRL010000250 DAJVRL010000000
VERSION     DAJVRL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.260"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            116..913
                     /locus_tag="OQN02_004072"
                     /pseudo
     CDS             116..913
                     /locus_tag="OQN02_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          400 a          239 c          307 g          378 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga tggtttatct ggtgggttaa ttgatggtct
      901 gaagatgctt gctgtggctg ttactgctca aaaggctgca aatgtagcag gaggcttgtt
      961 aaaccttgca aacgctgtta cttcattcgg tgacgctgtt aataaagcta aagctggcaa
     1021 ggctggttct tcaggtggtg cgctaaataa agtcggtaga gttgtccttt atgctgagtt
     1081 ggtaaatttt gctcttgata atttaaaccc tgaagcagct aagaatccga acttacatca
     1141 atcaggtatt ggcacagaaa caaacttcaa ccctgaatac aaaggtgatt catctaagcc
     1201 aatagttcta tcgctgactg gtttaatggt gcaactaata aaattgctaa cttccaagaa
     1261 ccgatgaata acttcgtaaa taacacacaa tcaactccat cagctatcac aggtgacatg
     1321 gtta
//
LOCUS       DAJVRL010000251         1293 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVRL010000251 DAJVRL010000000
VERSION     DAJVRL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.261"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(34..>1293)
                     /locus_tag="OQN02_004073"
     CDS             complement(34..>1293)
                     /locus_tag="OQN02_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HCS1644661.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE
                     QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEI
                     ADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ
                     AAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI
                     DQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQT
                     PSRPASEQPPAQPRLRITEQDPNWETF"
BASE COUNT          270 a          359 c          351 g          313 t
ORIGIN      
        1 tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat gtttcccagt ttggatcttg
       61 ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca ctggcaggac gggatggtgt
      121 ttgcggttta ttggtgagtg ggctggctgc cagacggaac gcggaaaccg cttgcgttaa
      181 acgactcgcc tgttcttcca gcgcagctgc ggcggcagct gattcctgca ccagcgatgc
      241 gttctgttgc gtgacgcgat ccatttccga aaccgccaat gcgacttgat cgatgccacg
      301 gctctgttca tccgatgccg atgcaatctc gcccataatg tcagtcacgc gagtgacagc
      361 attgacgata ttgttcattg tttccccggc gctttcgacc agcaccgaac cggtatcaat
      421 gcgcgagacg gagtcttcaa tgagggcttt gatctctttt gccgcctggg cgctgcgact
      481 ggcaagatta cgcacttcac ccgccaccac ggcaaaacca cggccctgtt cacccgcacg
      541 cgcggcttca accgcggcat tcagcgcgag gatattagtc tggaaggcaa taccgtcgat
      601 aacgctgata atgtcggcaa ttttcttcga actatcggcg atctcatgca tcgttttcac
      661 tacgccatcc accactttgc cgccgtgctg ggcggtgtcg gaggcacttt gcgccagttg
      721 cgaggcctgg cgggcgttat cggcgttttg cttcactgtc gcggtgagct gctccatgct
      781 ggcggcagtt tcttccagcg cggatgcctg ctgttcagta cgggaggaaa gatcggtgtt
      841 gcccgccgca atttcacggg taccggcata gatggcatct gaaccttcgc ggacatgagt
      901 gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc tgcgccaggt cacccatttc
      961 actgcgcccg tcaatggtca gggtattcgc caggttacca ccggcgattt cgcaaatgtg
     1021 agcaataatt tttgccagcg gagtaagcaa catacggcga atgccgtacc acgccaccag
     1081 cagaatcaat accaccacca gcgcgataac cgccagttgc cactgggcaa atcggtaatc
     1141 atctgcgttg tcagtgacga tatcgcgata cagtttttca ctgctgaggg cgtactgagc
     1201 aaacgcttcg cccattgcat tttgcattcc ctgggttggc tgagcgaaat aagctccagt
     1261 attgccataa tcaagataat caatcagttc agt
//
LOCUS       DAJVRL010000252         1259 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVRL010000252 DAJVRL010000000
VERSION     DAJVRL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.262"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            34..435
                     /locus_tag="OQN02_004074"
     CDS             34..435
                     /locus_tag="OQN02_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1644662.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OQN02_004075"
     CDS             447..818
                     /locus_tag="OQN02_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1644663.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1259
                     /locus_tag="OQN02_004076"
     CDS             805..>1259
                     /locus_tag="OQN02_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1644664.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPV"
BASE COUNT          299 a          281 c          390 g          289 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatcttgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccggga tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtgga
//
LOCUS       DAJVRL010000253         1232 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVRL010000253 DAJVRL010000000
VERSION     DAJVRL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.263"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            4..969
                     /locus_tag="OQN02_004077"
     CDS             4..969
                     /locus_tag="OQN02_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HCS1644665.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
BASE COUNT          309 a          316 c          377 g          230 t
ORIGIN      
        1 gccatgacga tgaacctgca cactcagaca accgccccta aagcttcaca ggcgaccagc
       61 gtatccccgc tggagcagtc aggctcctca aaaacgaaat tttccagggg caaaagcaaa
      121 gcccgtgctg gcgcagtcaa aacagacctt taccagacag taacggacag catcattgca
      181 gccctggaaa gcggcgttaa gccgtgggtg tgtccgtggg ttcgcaacgg cgcagcggtg
      241 ggattaccgg ccaacttcag caccggcacg gcatacagcg gaattaatat catgctgctg
      301 tggtgtagtg cggcaaaaca gggttttcag gacgagcgct ggctgaccta caaacaggcg
      361 caggagctgg gcggccaggt tcgtaaaggt gaacacggca cgacggccat cttttacaag
      421 acgctggaga aagaggacga ggacggggaa atcgaaaaaa tcccgatgct gaaagccttt
      481 accgtgttta acgtggagca aatcgacggc ctgagcatcg agagcgtgcc gcagccggtt
      541 gccggtttcg atccgctgcc gcaggcggaa gcactcatga tccggagcgg ggcaaaaatc
      601 accgagcagg gcgtaaaggc gttctatcgt ccggccaccg atgaaatcgt cctgccggag
      661 cggttccgct tcgcggacgc ggcgaacttc tacgccaccg gcctgcatga gctggttcac
      721 tggacgggtg cagcgtcccg cctcaatcgt gaaaaaggca ataagtttgg ctctgaggcg
      781 tatgcgtttg aggaactgat cgccgagctg ggcagcgcgt ttctgttggc ggatttgggg
      841 attacgggag aggtgcagca tgaaagctat atcgcgtcct ggctgaaagc gctgaaggga
      901 gacaagcgct acatctttaa agcggcggca gcggcctcaa aagcgcaccg ctggctgatg
      961 gactgctgac agctggagcc ctgcggtgca gggcttcaga atttttgaaa gagagagaat
     1021 cagggaggga attatataag tcactgtttt ttagaattct gtaacgcggc cggcctcagc
     1081 tgcaggacgt cctgcagctg aggccggcac ggaaaaataa accggacaga ttcaaaaaaa
     1141 atgaaccctt tttcaggcgg tagccgttac gtatccggga tacgtaacgg cgcgatgtct
     1201 tacgtaaaaa atgacgtaac ggctttcgcc gc
//
LOCUS       DAJVRL010000254         1202 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVRL010000254 DAJVRL010000000
VERSION     DAJVRL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.264"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>1202
                     /locus_tag="OQN02_004078"
     CDS             join(<1..299,299..>1202)
                     /locus_tag="OQN02_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCS1644666.1"
                     /translation="PEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWR
                     KQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYGRGKK
                     VDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHH
                     VIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVPPSKRAH
                     TGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQD
                     VMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRAC
                     NGLSDNRCL"
BASE COUNT          295 a          294 c          365 g          248 t
ORIGIN      
        1 gccggagaaa cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt
       61 tgaaccgggg atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt
      121 atttctctgg cgtaagcaat accaggaagg aagtcttact gctgtggccg ccggagaaca
      181 ggttgttcct gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct
      241 gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc
      301 aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc
      361 cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cgatgactgg
      421 atggatggcc gccgcagtcg tcacactgat gatacggatg tgcttctccg tatacaccat
      481 gttatcggag agctgcccac gtatggttat cgtcgggtat gggcgctgct tcgcagacag
      541 gcagaacttg atggtatgcc tgcgatcaat gccaaacgtg tttaccggct catgcgccag
      601 aatgcgctgt tgcttgagcg aaaacctgct gtaccgccat cgaaacgggc acatacaggc
      661 agagtggccg tgaaagaaag caatcagcga tggtgctctg acgggttcga gttctgctgt
      721 gataacggag agagactgcg tgtcacgttc gcgctggact gctgtgatcg tgaggcactg
      781 cactgggcgg tgactaccgg cggcttcaac agtgaaacag tacaggacgt catgctggga
      841 gcggtggaac gccgcttcgg caacgatctt ccgtcgtctc cagtggagtg gctgacggat
      901 aatggttcat gctaccgggc taatgaaaca cgccagttcg cccggatgtt gggacttgaa
      961 ccgaagaaca cggcggtgcg gagtccggag agtaacggaa tagcagagag cttcgtgaaa
     1021 acgataaagc gtgactacat cagtatcatg cccaaaccag acgggttaac ggcagcaaag
     1081 aaccttgcag aggcgttcga gcattataac gaatggcatc cgcatagtgc gctgggttat
     1141 cgctcgccac gggaatatct gcggcagcgg gcttgtaatg ggttaagtga taacagatgt
     1201 ct
//
LOCUS       DAJVRL010000255         1164 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVRL010000255 DAJVRL010000000
VERSION     DAJVRL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.265"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            97..279
                     /locus_tag="OQN02_004079"
     CDS             97..279
                     /locus_tag="OQN02_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCS1644667.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(380..709)
                     /locus_tag="OQN02_004080"
     CDS             complement(380..709)
                     /locus_tag="OQN02_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCS1644668.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(881..>1164)
                     /locus_tag="OQN02_004081"
     CDS             complement(881..>1164)
                     /locus_tag="OQN02_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644669.1"
                     /translation="FVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          303 a          265 c          268 g          328 t
ORIGIN      
        1 cgataccgtt cagacggcag gtgcctgctt ttccggcaac aagcaggaat ctcagcgctg
       61 aagcaggaat tagaggtaaa aacgccctac cgggccatga atcacccggt cattggagta
      121 gtaaccaaag cagatttagc cagcatggaa caaatatctt tggtgaaaag ttggttacgg
      181 gaagctggag cgcacaacgt gttagtaacc agtgcagtta ataacaatgg agttacagaa
      241 ctttttgcct tgctgcatac agaagaaggc tgttgttaat ttcgattggt tctcagatga
      301 gaatgatgac ggtgatacac agataagaat caatgagcaa tgagggttgc cgggcaaccc
      361 tcattgaata aaacgggaat tacttcgctt cgccgttttt catttcgccc atagctttca
      421 gctttttgga gatatcgcgg cgttctttgg agagctcggc atttttgatg atgtaatcgt
      481 caacgcgatc ttcatagtca cctttcatgc tggcgatgat gccctggatt gcttcaacgc
      541 tcatccctgg cttgatgtaa tcgctcaggt tgtccagcaa caggacgcgc ttctggttgt
      601 cacggatctt tttctcaacg tcctgaattt cacgttgcag tttgttctta cgacggaaca
      661 gacgaacaaa ttccagtacg tcctggaatg aaggcttggt agtttccatt tttatacccc
      721 tgataatgtg agagtcggat tcgtttaagc aaccgctatt cgttagggcc aacattactc
      781 atagctgccg cagatgacaa tgcttttatc ccttttcact atcataccct ttatccttgc
      841 tgaatcgaag cagcagcaag atgattctga agttcagaaa ttatttgcgc aaggcccggc
      901 aaatcagatt cgatagcaac tcaagctgat gtgccgtttc catgttcagc cacacataac
      961 catagattgg tgtttccacg actttcaggt catggtggtt atcgagcaac tgacgcagct
     1021 cttccacagc atcgttcaat ttttccgtat tggcaaaaac cggctgcgga ttaccttcgt
     1081 acagcgcatg aacaaggctc agcagtattt gctgcatcat gtgctgggta tcacaaagtt
     1141 tttgcgcgtt caataacacg aaat
//
LOCUS       DAJVRL010000256         1162 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVRL010000256 DAJVRL010000000
VERSION     DAJVRL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.266"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            115..>1162
                     /gene="cadB"
                     /locus_tag="OQN02_004082"
     CDS             115..>1162
                     /gene="cadB"
                     /locus_tag="OQN02_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCS1644670.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASAILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASD"
BASE COUNT          246 a          257 c          299 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcagctatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga cc
//
LOCUS       DAJVRL010000257         1146 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVRL010000257 DAJVRL010000000
VERSION     DAJVRL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.267"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            2..385
                     /locus_tag="OQN02_004083"
     CDS             2..385
                     /locus_tag="OQN02_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1644671.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="OQN02_004084"
     CDS             complement(386..1030)
                     /locus_tag="OQN02_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1644672.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          291 a          261 c          275 g          319 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcattgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattga
//
LOCUS       DAJVRL010000258         1133 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVRL010000258 DAJVRL010000000
VERSION     DAJVRL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.268"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            343..>1133
                     /gene="uacT"
                     /locus_tag="OQN02_004085"
     CDS             343..>1133
                     /gene="uacT"
                     /locus_tag="OQN02_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1644673.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGLMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAV"
BASE COUNT          253 a          263 c          257 g          360 t
ORIGIN      
        1 ttttgcgact gagttcaaat tattgcccct caatctatgc cgctttcatt atttcaacat
       61 tgccgatcaa aagagcgcta tccagataaa aacgattaaa tgcgagagtg cgacatgcca
      121 gaatgattaa tgaaatatca atatgagaaa attacagata attccattgt caggttctct
      181 tgtcattttc gtggtttaag cacgattatg tgattgcctc cgcaattcct gtctctaact
      241 ccccttcctc gcaaaaactg gcactccacg agcatgtgtt tagacagttt cattaacgta
      301 aacggttgct ttttattctg gcgggcgaaa ggagaaacac tgatgagcgc catagattcc
      361 caacttccct catcttctgg gcaagaccgc ccaactgatg aggttgaccg catattatca
      421 ccaggaaagc tgatcatact cggtctgcaa cacgtccttg tcatgtacgc aggtgcagtc
      481 gctgttcctc ttatgattgg tgaccgactg ggcctctcaa aagatgctat tgcgatgctc
      541 attagctcgg atctcttttg ctgcgggatc gtcacattat tgcaatgtat cggtatcggc
      601 cgctttatgg ggatccgcct gccggtgatt atgtcggtga cctttgctgc tgtaacacca
      661 atgatagcca ttgggatgaa cccggatatc ggcctgctgg ggatatttgg tgccactatc
      721 gccgcgggtt ttatcaccac attattagcg ccacttattg gtcgcttgat gcctttattc
      781 ccgccactgg ttaccggtgt ggttattact tctattgggc ttagcatcat tcaggtgggt
      841 attgactggg ccgctggagg taaagggaat ccgcaatatg gtaatcccgt ttatttaggt
      901 atctcctttg ccgtcttaat ttttatcttg ctcattactc gctatgcgaa aggattaatg
      961 tccaacgtcg ccgtattact ggggattgta tttggctttt tactttcgtg gatgatgaat
     1021 gaagtcaatt tatccgggct acatgatgcc tcgtggtttg cgattgtcac cccgatgtcg
     1081 tttggtatgc cgattttcga tcccgtttcc attctgacca tgactgccgt gtt
//
LOCUS       DAJVRL010000259         1110 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVRL010000259 DAJVRL010000000
VERSION     DAJVRL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.269"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..1019
                     /locus_tag="OQN02_004086"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OQN02_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DAJVRL010000260         1069 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVRL010000260 DAJVRL010000000
VERSION     DAJVRL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.270"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            18..560
                     /locus_tag="OQN02_004087"
     CDS             18..560
                     /locus_tag="OQN02_004087"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644674.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            787..>1069
                     /locus_tag="OQN02_004088"
     CDS             787..>1069
                     /locus_tag="OQN02_004088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCS1644675.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          258 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggtg
//
LOCUS       DAJVRL010000261         1061 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVRL010000261 DAJVRL010000000
VERSION     DAJVRL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.271"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..280)
                     /gene="tnpA"
                     /locus_tag="OQN02_004089"
     CDS             complement(<1..280)
                     /gene="tnpA"
                     /locus_tag="OQN02_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1644676.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGIS"
     gene            288..>1061
                     /locus_tag="OQN02_004090"
     CDS             288..>1061
                     /locus_tag="OQN02_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1644677.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVS"
BASE COUNT          303 a          251 c          256 g          251 t
ORIGIN      
        1 tcgatatgcc tttgaggctg tttaccagac tggatatcgc cagtttggga ggatagttaa
       61 ttaacaaatg gacgtgatct ggttcgccat ccatttcaac cagttcagct gcaaaatcag
      121 cacatacatt tgaaaagtaa gtgcgtagtt tttctgtcgt gtcatggtca aaaatctggc
      181 gtcggtattt ggtgacaaag accaggtgaa catgcatcag gaaaacacaa tgccttccac
      241 gccggatatc ggtttctttt ttcacagacc aaagtatcat ttgacctgtg aaacgattac
      301 aggcatttaa attccagtta agaccaggtg gtcaacagga gcgtgaaatg aggcgcttcg
      361 caggcgcatg tcgtttcgtt ttcaatcgtg ctctggcgct tcagaatgag aatcatgagg
      421 ccggtaataa atacatccct tacgggaaaa tggcttcctg gctggttgag tggaaaaacg
      481 ccactgaaac gcaatggctt aaagattcgc cctcacagcc attgcaacag tcactgaaag
      541 accttgagcg ggcttacaaa aacttcttcc agaagcgggc tgcttttccc cgattcaaaa
      601 agcggggaca gaatgctgca ttccgctacc cgcaggacgt taagctcgat caggaaaaca
      661 gccgtatttt tctgccgaaa cttggctgga tgcgctaccg gaatagccgt caggtcacgg
      721 gagttgtgaa aaatgtcact gtcagccagt cctgcggtac gtggtacatc agtattcaga
      781 cagaaagtga agtatcaact ccggttcacc cttcagcatc aatgatcggg ctggatgctg
      841 gcgtggctaa actcgccacg ctgtcagatg gcacagtctt tgagcctgta aacagttttc
      901 aaaaaaacca gaagacgctg gcgagacttc agcgacagtt aagccggaag gtcaaattca
      961 gcaacaactg gcagaagcag aaacgcaaaa tacagcgact gcattcctgt atcgcaaata
     1021 tccgcaggga ctaccttcac aaagtcacaa cgaccgtcag c
//
LOCUS       DAJVRL010000262         1041 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVRL010000262 DAJVRL010000000
VERSION     DAJVRL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.272"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..769
                     /locus_tag="OQN02_004091"
     CDS             <1..769
                     /locus_tag="OQN02_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1644678.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKPG"
     gene            790..>1041
                     /locus_tag="OQN02_004092"
     CDS             790..>1041
                     /locus_tag="OQN02_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCS1644679.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQS"
BASE COUNT          337 a          232 c          234 g          238 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac
      841 ggattgctga aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt
      901 gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa
      961 atactgctca gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca
     1021 ttactgactt acctgcaaag c
//
LOCUS       DAJVRL010000263         1010 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVRL010000263 DAJVRL010000000
VERSION     DAJVRL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.273"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(50..190)
                     /locus_tag="OQN02_004093"
     CDS             complement(50..190)
                     /locus_tag="OQN02_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644680.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="OQN02_004094"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="OQN02_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1644681.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          292 a          205 c          235 g          278 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt
//
LOCUS       DAJVRL010000264         1000 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVRL010000264 DAJVRL010000000
VERSION     DAJVRL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.274"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..1000
                     /locus_tag="OQN02_004095"
                     /pseudo
     CDS             1..1000
                     /locus_tag="OQN02_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          266 a          248 c          289 g          197 t
ORIGIN      
        1 gttgttgacc agaaatacac ggtggcagat gccgccaaag ctatggatgt tggcctttcc
       61 acaatgacaa gatgggtcaa acaactgcgt gatgagcgtc agggcaaaac accaaaagcc
      121 tcccccatta ccccggaaca aattgaaatc cgtgagctca ggaaaaagct acaacgcatt
      181 gaaatggaga atgaaatatt aaaaaaggct accgcgctct tgatgtcaga ctccctgaac
      241 agttctcgat aatcgggaaa ctcagagcgc attatcctgt ggtcacactc tgccatgtgt
      301 tcggggttca tcgcagcagc tacagatact ggaaaaaccg tcctgaaaaa ccagacggca
      361 gacgggctgt attacgcagt caggtacttg agctacatgg catcagccac ggttcggccg
      421 gagcaagaag catcgccaca atggcaaccc ggagaggcta ccagatggga cgctggcttg
      481 ctggcaggct catgaaagag ctggggctgg tcagctgtca gcagccgact caccggtata
      541 aacgtggtgg tcatgaacat gttgctatcc ctaactacct tgaaaggcag ttcgccgtga
      601 ccgagccaaa tcaggtgtgg tgcggtgatg tgacctatat ctggacgggt aagcgctggg
      661 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
      721 tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa acccgtggta
      781 agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc aggcagttcc
      841 ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga aactgctggg
      901 ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg ccggtggtgg
      961 gttacgtaag cttcagcgag gcagctcacg ccataacgga
//
LOCUS       DAJVRL010000265          999 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVRL010000265 DAJVRL010000000
VERSION     DAJVRL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.275"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..119
                     /locus_tag="OQN02_004096"
     CDS             <1..119
                     /locus_tag="OQN02_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1644682.1"
                     /translation="MLTGGTFWFVTELFILFALTVVIFMAKKIWLERQKRND"
     gene            119..667
                     /locus_tag="OQN02_004097"
     CDS             119..667
                     /locus_tag="OQN02_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1644683.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            677..>999
                     /locus_tag="OQN02_004098"
     CDS             677..>999
                     /locus_tag="OQN02_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1644684.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT          212 a          249 c          295 g          243 t
ORIGIN      
        1 gcatgttaac aggcggtact ttctggtttg tcaccgaact gtttattctg tttgccctga
       61 ccgtggtgat ttttatggcg aagaaaatat ggcttgagcg ccagaagagg aatgactgat
      121 gggtttaccg ccgcttagca aaattccttt cattttacgt ccgcaggcgt ggctgcatcg
      181 tcgccattac ggcgaggtgc taagcccgat tcgctggtgg gggcggatcc cgtttatctt
      241 ttatttggtg tcaatgtttg ttgggtggct ggagcgcaaa cgctcaccgc tcgatccggt
      301 agtgcgatcg cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt tttgcgtgga
      361 tatcaccagt atgaaagtcg ccgagcgtac cggcagcacc gataaactgc tggcagtggc
      421 tgactggcgg caaagcccgc tctttagcga tgaagaacgg ctggcgctgg agtacgccga
      481 agccgcaagc gtcacaccgc caacagtcga tgatgccctg cgtacccgac tggctgcgca
      541 ttttgacgct caggcgctca ccgaactgac ggcattgatc ggcctgcaaa acctgtcggc
      601 tcgttttaat tctgccatgg acattcccgc tcaggggctg tgccgtattc cggaaaaacg
      661 ttcttaagga gggaagatgc gccattgtgg gtggttgctg ggattgttat cgctgttttc
      721 tttggcaaca catgccagtg actggcagga aattaaaaat gaggccaaag ggcaaaccgt
      781 ctggtttaac gcctggggcg gcgataccgc aattaaccgc tatctcgact gggttagcgg
      841 cgagatgaaa acccattacg ctataaacct gaagattgtt cgtctcgcgg atgccgcaga
      901 cgcggtgaag cgcattcaga ccgaagctgc tgccggtcgt aaaacgggcg gctcggtgga
      961 tctgctctgg gtgaacggcg aaaacttccg caccttaaa
//
LOCUS       DAJVRL010000266          973 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVRL010000266 DAJVRL010000000
VERSION     DAJVRL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.276"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OQN02_004099"
     CDS             complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OQN02_004099"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS1644685.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          257 a          219 c          217 g          280 t
ORIGIN      
        1 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
       61 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
      121 attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac cttcccccat
      181 agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa tacgagcaat
      241 aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga tatgaatacc
      301 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
      361 gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca atgggatcgt
      421 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg
      481 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
      541 aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca atttgtcttt
      601 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
      661 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc
      721 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttcagacg
      781 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
      841 ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa acgcagagga
      901 tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag cgttaactcg
      961 aggcttctta ata
//
LOCUS       DAJVRL010000267          973 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVRL010000267 DAJVRL010000000
VERSION     DAJVRL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.277"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..102
                     /locus_tag="OQN02_004100"
     CDS             <1..102
                     /locus_tag="OQN02_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS1644686.1"
                     /translation="RVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            99..653
                     /locus_tag="OQN02_004101"
     CDS             99..653
                     /locus_tag="OQN02_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1644687.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            794..>973
                     /locus_tag="OQN02_004102"
     CDS             794..>973
                     /locus_tag="OQN02_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1644688.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
BASE COUNT          253 a          215 c          269 g          236 t
ORIGIN      
        1 cgggtaaaaa ccagcgatta caccaaagaa gaattacgcc tgatgcatgc agaaggtgtt
       61 tttcgcacac aagaaatctg gagaaaggaa ggttatttat gatttaccca acaaatacag
      121 gcaaaagcgg ggaacacctt cgtctcacca cgctggaaag tgtctggatt cagggaaaac
      181 tgcgcatgtg ggggcgctgg tcgtatattg gcggcggtaa gacgggaaat atgtttaacc
      241 agttgctggc atccaaaaaa ttgacgaaaa cagccgtcaa tgaagccctg cgcaggatga
      301 aaaaagcggg aatagagaaa cctgagctgg aagcgttttt gcgagagatg atcaatggca
      361 agcaaaagac ctggctggcg cattgtactg atgcagaggc gttatgtatt gatagagtca
      421 taagtgaggt gctggcagag catcctggat tgattagtgt cctccggcaa cggtatgaag
      481 ggcgggggat gactaagcgt aaaatggctg aattgctgaa tgacgcacac cctgagtggt
      541 gtttccggac gtgctgcagt cgggtagatg tatggctaaa tcttgctgaa tatatgctct
      601 atctaccgat gcgtgatgca ttctcttccg gggatctaaa aaccgtttgt tgactcaatc
      661 tgttatccgg ggctatattc ctcacgcgcc agcaaaatct ggcgtcggga ttggcgtccc
      721 ggatgtttac ggagcgatat gagacgcgcc cgcgtctttt ttcatatcgt ttgcacagtc
      781 acattcgcga tttatggcgg gctgtgtggg ggagccgaaa ggctcgccgg tttccgtacc
      841 cggttacgcc aaccctgcac agttcgccac cagttcgatt ggcgtcgtcg gtggcgataa
      901 tttccaaatg tacggagtta tcgttatgac cactcaaatc tctgttgaaa ctctctcccc
      961 gatcacccat aac
//
LOCUS       DAJVRL010000268          938 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVRL010000268 DAJVRL010000000
VERSION     DAJVRL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.278"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(77..>938)
                     /gene="mukB"
                     /locus_tag="OQN02_004103"
     CDS             complement(77..>938)
                     /gene="mukB"
                     /locus_tag="OQN02_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="SMC (structural maintenance of chromosomes) family
                     of proteins; involved in chromosome condensatin and
                     partitioning; forms a homodimer and the C-terminal is
                     essential for DNA-binding activity while the purified
                     N-terminal domain binds FtsZ; mutations result in cell
                     division defects; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1644689.1"
                     /translation="DIIRTDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANII
                     RKTIQREQNRIRMLNQGLQNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFN
                     SNRLTFSEALAKLYQRLNPQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRA
                     ESGALSTGEAIGTGMSILVMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIAT
                     LFELCERLQMQLIIAAPENISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLL
                     GRDEAPSQAS"
BASE COUNT          217 a          253 c          233 g          235 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcc
//
LOCUS       DAJVRL010000269          887 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVRL010000269 DAJVRL010000000
VERSION     DAJVRL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.279"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(55..243)
                     /locus_tag="OQN02_004104"
     CDS             complement(55..243)
                     /locus_tag="OQN02_004104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644690.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          226 a          170 c          211 g          280 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagat
//
LOCUS       DAJVRL010000270          858 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVRL010000270 DAJVRL010000000
VERSION     DAJVRL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.280"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..126)
                     /locus_tag="OQN02_004105"
     CDS             complement(<1..126)
                     /locus_tag="OQN02_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1644691.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGH"
     gene            complement(123..797)
                     /locus_tag="OQN02_004106"
     CDS             complement(123..797)
                     /locus_tag="OQN02_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCS1644692.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRLAKQALQPGAVVARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE
                     MKGGIR"
BASE COUNT          201 a          211 c          222 g          224 t
ORIGIN      
        1 atgcccggag aacgggtcat ctttcagcgt gttctgcacc ttcgaagcca ggccattgaa
       61 gccacaacgc atatctgtga tgccagcgat gatccagatt ttggtaccgg tcggcagcgt
      121 tatcatcgga tacccccttt catttcgcgg attagcgccc gtaacagttc cggagtgaga
      181 gggtcaaaca gttttaccac acctgattta agatgcagct cgcaccgtgg gacgtttccg
      241 ggagccccct cagggcgctc atcatgcttg ttacgccaga agggatttgt aactggtctg
      301 gtcggctccg gcgtatcagt cagtgccacc gggacaggca tgcattcctg tatgtcatca
      361 tcgctcagta agccgtcctc gtactggctt ttccatttaa acagcaggtt attattgata
      421 tcgtgttctc tggcgatccg ggcaacaaca gccccaggct gcaatgcctg cttagccaga
      481 cggaccttaa attcacggct atagctggtt cgccgttctt ttcgccatga gccttctctg
      541 atttgaggct ctgttaattc cttctttctg ttggcataaa ggatggcgtc aagttgagcg
      601 aatgaaactg aatcgggcaa tggccatgcg ataccggatg caagaaatcg ctgaaaaagc
      661 gtatgtattg tggaatgact gagacccaga cgctgagcga tggcccggat ggtcagttta
      721 tcttcaaatc ttaaacgcag ggcatcaggc aaataagaac ggaagcaggg aatatctttt
      781 gttgtctggg aattcatcgt tcgtgtccat caatatagat gggcgcgatt gttgccagac
      841 aggacaattt tcacaaga
//
LOCUS       DAJVRL010000271          851 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVRL010000271 DAJVRL010000000
VERSION     DAJVRL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.281"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..598
                     /gene="phnD"
                     /locus_tag="OQN02_004107"
     CDS             <1..598
                     /gene="phnD"
                     /locus_tag="OQN02_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1644693.1"
                     /translation="RKDLTFGNGDPNSTSGFLVPGYYVFAKNNISASDFKRTVNAGHE
                     TNALAVANKQVDVATNNTENLDKLKTSAPEKLKELKVIWKSPLILGDPIVWRKNLSET
                     TKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASSDLQLVPIRQLALFKEIQSVKGNKG
                     LNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVSKAVQ"
     gene            704..>851
                     /locus_tag="OQN02_004108"
     CDS             704..>851
                     /locus_tag="OQN02_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCS1644694.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT          219 a          248 c          232 g          152 t
ORIGIN      
        1 gcggaaagat ctcaccttcg gcaatggcga tcctaactcc acctctggct tcctcgtccc
       61 cggttactac gtcttcgcta aaaacaatat ctccgccagc gacttcaagc gcaccgtcaa
      121 cgccgggcat gaaaccaacg cgctggccgt cgccaacaag caagtggatg ttgccaccaa
      181 caacaccgaa aacctcgaca agctgaaaac ctccgcgccg gagaagctta aagagctgaa
      241 ggtgatctgg aaatcgccgc tgatcctagg cgatccgatc gtctggcgca agaatctgtc
      301 cgaaactacc aaagacaaga tctacgactt ctttatgaac tacggaaaaa cgccggaaga
      361 gaaagcggtg ctggaacgcc tgggctgggc gccgttccgc gcttccagcg acctgcaact
      421 ggtgccgatt cgccagctcg cactgtttaa agagatacaa agcgtgaaag gcaataaagg
      481 gctgaatgag caggacaagc tggcgaaaac caccgagatt caggcgcagc tggatgacct
      541 ggaccgcctg aacaacgcgt taagcgcgat gagttcggtg agtaaagcgg tgcagtaaat
      601 cgtaggtcgg ataagacgcc ccggcgtcgc atccgacaat gtgtaggcgt tgatgccgga
      661 tgcggtgcaa gcaccttatc cggcctacag accggagcca aacatgcaaa ccatcaccat
      721 cgccccaccc aagcgtagct ggttctcgct tctgagctgg gccgttgttc tcgacgtgct
      781 ggtcgtctcg tggcagggcg cggaaatggc cccgcttacg ctgattaaag acggcggcaa
      841 catggcaacc t
//
LOCUS       DAJVRL010000272          833 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVRL010000272 DAJVRL010000000
VERSION     DAJVRL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.282"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..661)
                     /gene="proB"
                     /locus_tag="OQN02_004109"
     CDS             complement(<1..661)
                     /gene="proB"
                     /locus_tag="OQN02_004109"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1644695.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QA"
BASE COUNT          192 a          232 c          210 g          199 t
ORIGIN      
        1 cggcctgcaa tttagtactc atgccgccag ttccgaggcc tgaaacgctg tcaccggcaa
       61 tcgcgcgcag tgcgtcatca atgccgtaaa catctttaat cagttctgcc tgcggatttc
      121 tgcgcggatc agcggtatac aaaccttttt gatcggtcag cagcaacagt ttatcggcac
      181 ccgccagaat cgccgccagc gcagaaaggt tatcgttatc gccgacctta atctctgccg
      241 tagcgacagc atcgttctca ttgattaccg gaacgatatt gttatcgagc aacgcacgca
      301 gggtgtcgcg ggcgttcagg aagcgttcac ggtcttccat atcagcacgg gtcagcagca
      361 tttgcccgac gtgaatgcca taaatcgaaa acagctgttc ccacagttga atcagtcgac
      421 tctgccctac cgccgccagc agttgtttcg aggcgatagt cgctggcagt tccgggtaac
      481 ccaggtgctc acgtccggcg gcgatcgcgc ccgacgtcac aataacaatc cgatgcccgg
      541 cggcatgtaa ctgcgcgcac tggcgaacaa gttcaacgat atgggcacgg ttcagacggc
      601 gcgatccgcc tgttagcaca ctggtgccga gttttaccac cagcgtctgg ctgtcactca
      661 tgattctctg ccattcaatt ttaggaaaaa tgatatcaaa cgaacgtttt agcaggactg
      721 tcgtcggttg ccaaccatct gcgagcaaag catggcgttt tgttgcgcgg gatcagcaag
      781 cgtagcggca gttgtttacg cttttattac agatttaata aattaccaca ttt
//
LOCUS       DAJVRL010000273          832 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVRL010000273 DAJVRL010000000
VERSION     DAJVRL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.283"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..327
                     /locus_tag="OQN02_004110"
     CDS             <1..327
                     /locus_tag="OQN02_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1644696.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(370..570)
                     /gene="glgS"
                     /locus_tag="OQN02_004111"
     CDS             complement(370..570)
                     /gene="glgS"
                     /locus_tag="OQN02_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCS1644697.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          155 g          241 t
ORIGIN      
        1 gttagtgaaa cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa
       61 ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag ccatctggat
      121 atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac
      181 cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc
      241 agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac
      301 gtaatcctca aaatcaccta taactagcat ctaatacaaa cactaaaacg ggccatcagg
      361 cccgttatat cagtgctcta actccagctc tcttgcctgc atcatctgtt ggcaatacca
      421 ggcataacgt gcgcaaaacc aggttctatg ttcttcatcc atgttaccag taacagccag
      481 aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcca ggaaatcgaa
      541 attattcaaa gaattaagac tatgatccat aagcacatcc tccggttatc gcataccttt
      601 taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc ctttaaacat
      661 aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa aaaccatatt
      721 aaaatacttt ctcttccaga acttctctaa catgtgttat tattaacaaa gacaaatgta
      781 agcagggaaa ctattccaag gtaaatattg cgtcactaaa tggacattgg ag
//
LOCUS       DAJVRL010000274          811 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVRL010000274 DAJVRL010000000
VERSION     DAJVRL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.284"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>811
                     /locus_tag="OQN02_004112"
     CDS             <1..>811
                     /locus_tag="OQN02_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1644698.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DAJVRL010000275          805 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVRL010000275 DAJVRL010000000
VERSION     DAJVRL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.285"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            154..477
                     /locus_tag="OQN02_004113"
     CDS             154..477
                     /locus_tag="OQN02_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCS1644699.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..>805
                     /locus_tag="OQN02_004114"
     CDS             474..>805
                     /locus_tag="OQN02_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1644700.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRF"
BASE COUNT          206 a          184 c          224 g          191 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt ttttt
//
LOCUS       DAJVRL010000276          802 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVRL010000276 DAJVRL010000000
VERSION     DAJVRL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.286"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            206..421
                     /locus_tag="OQN02_004115"
     CDS             206..421
                     /locus_tag="OQN02_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644701.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            524..802
                     /locus_tag="OQN02_004116"
                     /pseudo
     CDS             524..802
                     /locus_tag="OQN02_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          215 a          189 c          193 g          205 t
ORIGIN      
        1 gtgagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg ggtttttgtt tttattttca acgcatttga agttctggat ggtgccggaa
      121 tagaatcaaa aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga
      181 gtgctactta tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg
      241 tgaagatggc ctttatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat
      301 tgtgtgtttg caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga
      361 cggatttatc ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatctg
      421 aataaaagct ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg
      481 gctgaactcc cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa
      541 atattacaat catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc
      601 atttaagtca gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat
      661 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
      721 ctagataatc tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt
      781 ccagcaacgg atctggaggt aa
//
LOCUS       DAJVRL010000277          797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVRL010000277 DAJVRL010000000
VERSION     DAJVRL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.287"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            42..797
                     /locus_tag="OQN02_004117"
                     /pseudo
     CDS             42..797
                     /locus_tag="OQN02_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          224 a          193 c          196 g          184 t
ORIGIN      
        1 tatcggtttc ttttttcaca gaccaaagta tcatttgacc tgtgaaacga ttacaggcat
       61 ttaaattcca gttaagacca ggtggtcaac aggagtgtga aatgaggcgc ttcgcaggcg
      121 catgtcgttt cgttttcaat cgtgctctgg cacgtcagaa tgagaatcat gaggccggta
      181 ataaatacat cccttacggg aaaatggctt cctggctggt tgagtggaaa aatgccactg
      241 aaacgcaatg gcttaaagat tctccctcac agccattgca acagtcactg aaagaccttg
      301 agcgggctta caaaaacttc ttccggaagc gggctgcttt tccccgattc aaaaagcggt
      361 gacagaatga tgcattccgc tacccgcagg gcgttaagct cgatcaggaa aacagccgta
      421 tttttctgcc gaaactgggc tggatgcgct accggaatag ccgtcaggtc acgggtgttg
      481 tgaaaaatgt cactgtcagc cagtcctgcg gtacgtggta catcagtatt cagacagaaa
      541 gtgaagtatc cactccggct cacccttcag catcaatggt cgggctggat gctggcgtgg
      601 ctaaactcgc cacgctgtca gatggcacag tctttgagcc tgtaaacagt tttcagaaaa
      661 accagaagac gctggcgaga cttcagcgac agttaagccg caaggtcaaa ttcagcaaca
      721 actggcagaa gcagaaacgc aaaatacagc gactgcattc ctgtatcgca aatatccgca
      781 gggactacct tcacaaa
//
LOCUS       DAJVRL010000278          791 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVRL010000278 DAJVRL010000000
VERSION     DAJVRL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.288"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            16..435
                     /gene="rbsD"
                     /locus_tag="OQN02_004118"
     CDS             16..435
                     /gene="rbsD"
                     /locus_tag="OQN02_004118"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1644702.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            443..>791
                     /locus_tag="OQN02_004119"
     CDS             443..>791
                     /locus_tag="OQN02_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1644703.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          219 a          189 c          196 g          187 t
ORIGIN      
        1 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
       61 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
      121 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
      181 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
      241 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
      301 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
      361 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
      421 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
      481 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
      541 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
      601 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
      661 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
      721 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
      781 tgactggaaa a
//
LOCUS       DAJVRL010000279          779 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVRL010000279 DAJVRL010000000
VERSION     DAJVRL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.289"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(317..496)
                     /locus_tag="OQN02_004120"
                     /pseudo
     CDS             complement(317..496)
                     /locus_tag="OQN02_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            590..>779
                     /locus_tag="OQN02_004121"
     CDS             590..>779
                     /locus_tag="OQN02_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1644704.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAA"
BASE COUNT          211 a          142 c          201 g          225 t
ORIGIN      
        1 ctgatgatta tttatatccc ggattggtag ggaaaagctt ttttcatgca ctatttttgc
       61 aaatatttct atatgggaag tggttttgtt acagattaaa tttataaaga aatgcatttg
      121 gggtgttggg ttgaagagta gctggtagat gttattgtgc cagcaggggc aggggaatgt
      181 gccgtatcga tatgctttgg caattgcgtg tgccgcagat ggagatgaaa tgccaataaa
      241 aaagcctgag ttcgatgagg gacgaactca ggctggtgtt atgttttaca gtaacacgcc
      301 tcggcgcgtt ggctgattag aactggtaga cgataccaac tgccagttgg tcgtcggtgt
      361 tcagtccatg agcgagaaca taatcactct tatcaagcag gttgatctga taagcggtat
      421 atatgttcat attcttattg aagtaatacc aggtaccgat ttcaatatag ttcagacgat
      481 cagaatcttt gtaacctgta gactggcccc ctgaatctcc agacaaccaa tatcacttat
      541 ttaagtgata gtcttaatac tagtttttag actagtcatt ggagagcaga tgattgatgt
      601 cttaggaccg gagaaacgca gacggcgtac tacacaggaa aagatcgcta ttgttcagca
      661 gagttttgaa ccgggaatga cggtctccct tgttgcccgg caacacggtg tggcagccag
      721 ccagttattt ctctggcgca agcaatacca ggagggaagt cttactgctg tggccgcag
//
LOCUS       DAJVRL010000280          761 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVRL010000280 DAJVRL010000000
VERSION     DAJVRL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.290"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..557)
                     /gene="inaA"
                     /locus_tag="OQN02_004122"
     CDS             complement(<1..557)
                     /gene="inaA"
                     /locus_tag="OQN02_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS1644705.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLGRDKA"
BASE COUNT          194 a          218 c          146 g          203 t
ORIGIN      
        1 gctttatcgc gacccaagcg gcgacgactt ttctccagat caagaaaacc agcttctgcc
       61 ttaccttctg ttttcacata gatatggcga acatagcaac agccatgctg acgattaacg
      121 ctatgcattt tcttaaacgc cagtgccact gctttcaaca tggcttgccg tacttcgtca
      181 gaataaggcg atactgcatg ccgggcatac cagtcagcaa tgctgatgaa ccccgccata
      241 tcttcagtca ccagcaacgc gcgccattca ccctcaattt tcaccgcttc accaaaaacg
      301 atcttcggca cgatgacacc ggcccgttcc agttctttaa ttaccgcaac ctcacggaca
      361 atcgttggtc ggccgaacgg ataacgtact gaatgaaaca gatgatgcgt catgcgcttt
      421 acatacagct ttttgccgtt gcgctcgacg cattgcaccc cgctcatacc attacggcga
      481 tagttaggct cttcaaccca gtcgccctct gttgcccacc agtgattaaa ttcgtcgtac
      541 tttgctgaaa ctgccatacc ctatcgcctg tcatttttat taacgacaat gactataggt
      601 ggttacctga ggaaaatctt aatgaaacgt gtcgtattaa tgaatttctt ttgccgctac
      661 aggtgtgaca ccgcttttgc ccttcatttg cagcgcatag attccaccag ccactaccac
      721 taatagtgcg cccagcagat aaaatgtgaa tttaatcagt c
//
LOCUS       DAJVRL010000281          748 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVRL010000281 DAJVRL010000000
VERSION     DAJVRL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.291"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(62..138)
                     /locus_tag="OQN02_004123"
     tRNA            complement(62..138)
                     /locus_tag="OQN02_004123"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Arg,seq:tct)
     gene            complement(140..215)
                     /locus_tag="OQN02_004124"
     tRNA            complement(140..215)
                     /locus_tag="OQN02_004124"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Ile,seq:cat)
     gene            complement(392..>748)
                     /locus_tag="OQN02_004125"
     CDS             complement(392..>748)
                     /locus_tag="OQN02_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1644706.1"
                     /translation="SICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQ
                     AVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTR
                     SEQKAAFSKQSIAKVD"
BASE COUNT          182 a          196 c          155 g          215 t
ORIGIN      
        1 atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc taagtcggcg
       61 ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg atgctctatc
      121 cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac ctggcgatta
      181 tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata accatatgtc
      241 atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc atttttcagg
      301 cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac aaaatcggca
      361 aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata ctttgctttg
      421 aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc tcactatcca
      481 gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg gaccagtttt
      541 tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc ccaccaaaaa
      601 gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt tcctgtgttt
      661 tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc cagtgggtta
      721 tggtgatctg atactcccag caaatact
//
LOCUS       DAJVRL010000282          743 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVRL010000282 DAJVRL010000000
VERSION     DAJVRL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.292"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            2..526
                     /locus_tag="OQN02_004126"
     CDS             2..526
                     /locus_tag="OQN02_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644707.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            523..>743
                     /locus_tag="OQN02_004127"
     CDS             523..>743
                     /locus_tag="OQN02_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644708.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVL"
BASE COUNT          169 a          185 c          215 g          174 t
ORIGIN      
        1 catgaatcag acagcatttt tacacgtcga ctttaaacaa ccagaggagc tggagtttaa
       61 tcgcgcccgt ctgcgtcggg cgtttgtgca aatcgggcgc gtttacatgc gtgatgccag
      121 acgtctggtg attaaacgcg gacgctccgg tccgggagag aacccgggat atcagaccgg
      181 gcgtctggcc cgctccattg gttattacgt tcccaaaaaa acgacgcgtc gccccggact
      241 gatggtgaaa atttcccctg accagaaaaa cgggcagggg aatcgccgtt ttcctgaagg
      301 tgctccttat tatccggcgt ttctgtatta cggcgttcgc cattcagcgt atggaatgga
      361 taaaaaggat aagcggcaga agaagcacca ttcttcgact ttccggctgg ccccgcgtaa
      421 taactttatg gctgacgtta ttgagcgtcg gcgtcactgg acgcaggaac tgttgtcccg
      481 tgagctgcag cgttcgttac gtccggtaaa aaggaagcat aaatgaaact gacaacgata
      541 attgcggcac tgcgtgagcg atgcccgcgt tttgaggagc gtgttggtgg cgcggcgcag
      601 tttaaggcga tccctgatgc cggaaaactt cgcctgcctg ctgcctatgt ggttccctct
      661 gacgatgcgc cgggggaaca aaaatcacag accgattact ggcaggattt gactgaaggc
      721 ttctccgtga ttgtggtgct cag
//
LOCUS       DAJVRL010000283          719 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVRL010000283 DAJVRL010000000
VERSION     DAJVRL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.293"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..719
                     /locus_tag="OQN02_004128"
                     /pseudo
     CDS             1..719
                     /locus_tag="OQN02_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          207 a          153 c          183 g          176 t
ORIGIN      
        1 ctacttcact actgatgatg gttacaggat catctcggca cgttttggtg tcccccgaac
       61 ccaggtcagg acatgggttg ccctctatga aaaacatgga gaaaaaggtt taattcccaa
      121 acctaaaggc gttagtgctg atccagagtt gcgtattaag gtcgtgaaag ctgtgatcga
      181 gcagcacatg tcccttaatc aggctgctgc tcactttatg cttgctggta gtggttctgt
      241 agccaggtgg ctgaaggtct atgaagagcg cggagaagct ggtttacgcg cgctcaagat
      301 tagcaccaaa agaaacattg caatatcagt tgatccagaa aaagcggcat cagcattgga
      361 gctgtcaaaa gaccgacgca ttgaggatct tgaaaggcaa gttcgatttc ttgaaacgcg
      421 gcttatgtat ctaaaaaagc tgaaagcctt agctcatccc acgaaaaagt gaaagtactc
      481 aacgagctaa ggcagtttta tcctcttgat gagcttctca gggctgcgga gataccgcgc
      541 agtacgtttt attaccatct aaaggctctc agcaagcctg acaagtatgc ggacgttaaa
      601 aagcgtattg gtgagattta tcacgagaat agaggccgat acggataccg tagggtaacg
      661 ctgtctcttc atcgagaagg gaaacagatt aaccataaag ctgttcagcg cctgatggg
//
LOCUS       DAJVRL010000284          714 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVRL010000284 DAJVRL010000000
VERSION     DAJVRL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.294"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..76
                     /locus_tag="OQN02_004129"
     CDS             <1..76
                     /locus_tag="OQN02_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1644709.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..714
                     /locus_tag="OQN02_004130"
                     /pseudo
     CDS             92..714
                     /locus_tag="OQN02_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
BASE COUNT          228 a          172 c          190 g          124 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact
      421 cgcagaagca gtaagtgcgc aacaaaacgt agaaaccact cgccagaccg cagaagccga
      481 tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga
      541 actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt
      601 tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct
      661 gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca aact
//
LOCUS       DAJVRL010000285          709 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVRL010000285 DAJVRL010000000
VERSION     DAJVRL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.295"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            4..435
                     /locus_tag="OQN02_004131"
     CDS             4..435
                     /locus_tag="OQN02_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644710.1"
                     /translation="MTMNLHTPTTAPKAAPATSVSPLEQSGSPKTKFPEIKTVKTAVV
                     KLISDIEGNLDQRLVYREAVEGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTI
                     IVVEFEGEQTRLNYGGRSAEDVIAMLKALHLEGRKAWLEVE"
     gene            complement(467..>709)
                     /locus_tag="OQN02_004132"
     CDS             complement(467..>709)
                     /locus_tag="OQN02_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1669 domain-containing protein"
                     /protein_id="HCS1644711.1"
                     /translation="YLINQGVPVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSR
                     NAENVLLIEDAPQLAEAYQREFNRLWDEGIPLNALY"
BASE COUNT          170 a          202 c          202 g          135 t
ORIGIN      
        1 accatgacga tgaacctgca caccccgaca accgccccta aagctgcacc ggcgaccagc
       61 gtatccccgc tggagcagtc aggctcccca aaaacgaaat ttcctgaaat caaaaccgtc
      121 aaaacggcgg tggttaaact gatcagcgac atcgagggca acctcgatca gcgtctggta
      181 tatcgcgagg ccgtagaggg attgtgccag gaggcggcca accggataac ccagtgcatg
      241 cattcacgca gtacagggca gacgctacgt gatctccgcg agctgctgtc ggtgacgctg
      301 gacaccatta tcgtggtgga gtttgaaggg gagcagaccc gtctcaacta tggcggacgt
      361 tcagcagaag acgtgatcgc catgctgaaa gcgctgcatc ttgaaggacg caaagcctgg
      421 ctggaagtgg agtaaaaaaa aagccccgtc cgcagacggg gctgattcag tacagggcat
      481 tcagcgggat gccctcatcc cacaggcggt taaattctcg ctggtacgcc tccgccagct
      541 gcggcgcatc ctcgatcagc agcacgtttt ccgcgttgcg cgaaacggcg cttgccgtgt
      601 agttaaacga cccggtctgg acgtttttgc cgtcgatcac cataaacttg ttgtgcatga
      661 tggcgtaacg gccgttaagg tgcaccggca cgccctggtt tatcagata
//
LOCUS       DAJVRL010000286          708 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVRL010000286 DAJVRL010000000
VERSION     DAJVRL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.296"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            118..708
                     /locus_tag="OQN02_004133"
                     /pseudo
     CDS             118..708
                     /locus_tag="OQN02_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
BASE COUNT          181 a          193 c          215 g          119 t
ORIGIN      
        1 tcggcatcat ctgcgtctgc ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag
       61 ggcagtgcca cgacggcatc ctctaaggcg acagagtctg caggcagtgc gacggcggca
      121 gcgcagagca aaagcgcggc agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg
      181 gcagaggata ttgctgatgt cgtatctctg gaagatgcga gcacgacgaa aaaggggata
      241 gtgcagctca gcagtgcgac caacagtgca tccgagtcac tggccgcgac agcgaaagcg
      301 gttaaggtgg taatggatga aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc
      361 ggaacgccaa ccacacccac tgcgccaaag gggactaata atactcagat cgccagtacg
      421 gcttatgtta tggccgcgat tgctgccctc gtggactcgt cgcctgatgc actgaacacg
      481 ctgaatgaac tggcggcggc gctgggcaac gacccgaatt ttgcgaccac catgactaac
      541 gcgcttgcgg gtaagcaacc gaaagatgcc actctgacgg cgctggcggg gcttgctact
      601 gcggcagaca ggtttccgta ttttacgggg aatgatgtcg ccagcctggc aaccctgaca
      661 aaagtcgggc gggatattct tgcgaaatcg accgttgccg ccgttatc
//
LOCUS       DAJVRL010000287          695 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVRL010000287 DAJVRL010000000
VERSION     DAJVRL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.297"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            43..>695
                     /locus_tag="OQN02_004134"
     CDS             43..>695
                     /locus_tag="OQN02_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HCS1644712.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFY"
BASE COUNT          169 a          184 c          185 g          157 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaacgtg
      421 aagaagtggc agatcccgcg attcatcaac acggataaag cgcccgccta tggtcgcgcg
      481 cttgctctgc tcaaacgcga aggccggtgc ccgtctgacg ttgaacaccg acagattaag
      541 taccggaaca acgtgattga atgcgatcat ggcaaactga aacggataat cggcgccacg
      601 ctgggattta aatccatgaa gacggcttac gccaccatca aaggtattga ggtgatgcgt
      661 gcactacgca aaggccaggc ctcagcattt tatta
//
LOCUS       DAJVRL010000288          682 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVRL010000288 DAJVRL010000000
VERSION     DAJVRL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.298"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..682
                     /locus_tag="OQN02_004135"
                     /pseudo
     CDS             1..682
                     /locus_tag="OQN02_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          165 a          173 c          192 g          152 t
ORIGIN      
        1 aacgggttac cgacgaacgg gacatattaa aaaaagccgc ggcgtacttc gcaaagctgt
       61 ccgactgagg tacgccttta tccgtgacaa ctcctgttgc tggcctgttc gcctgctctg
      121 tcgggtgctg gatgttcatc ccagtggttt ttacgcctgg cttcagcagc cgcattcaca
      181 acgccatcag gcagacctga gactgacagg acagattaaa cagttctggc tggaatcggg
      241 atgcgtctat ggttatcgca aaatccatct ggatctgcgt gacagcgggc aacagtgcgg
      301 agtaaacaga gtctggagac tgatgaaacg tgtcggaata aaggctcagg tcggataccg
      361 aagcccgcgg gcacgtaaag gcgaggccag tatcgtgtca cccaacaggc tccagcgaca
      421 gttcaatccg gatgctcctg atgagcgttg ggtaacggac ataacctaca tcaggaccca
      481 cgaaggctgg ctgtatcttg ccgttgttgt tgatctgttc tcacgcaaaa ttatcggctg
      541 gtccatgcaa tcccggatga caaaggacat tgtcctgaac gcactgctga tggctgtatg
      601 gcggcgtaat cccgaaaaac aggtgctggt tcattcggat cagggcagtc agtacacaag
      661 ccatgagtgg cagtcgttcc tg
//
LOCUS       DAJVRL010000289          682 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVRL010000289 DAJVRL010000000
VERSION     DAJVRL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.299"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>682
                     /locus_tag="OQN02_004136"
     CDS             <1..>682
                     /locus_tag="OQN02_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1644713.1"
                     /translation="TLADNFKQKNEALDTWYVGMNDFARIAGGQNSRSNILSPRAFLE
                     FLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKFYMNNPEGERADLS
                     KVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDDSSLHLITNKVLAC
                     YQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNNSQVSCKGSVDSTI
                     TDLLEKAL"
BASE COUNT          238 a           99 c          147 g          198 t
ORIGIN      
        1 ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg tatgtgggaa
       61 tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt ctttctccta
      121 gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat tttagcaaac
      181 gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct tattctaaag
      241 atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg gagcgagcgg
      301 atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa gggattcgtg
      361 aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt tatgaaggga
      421 agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag gttctggcgt
      481 gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat cagaagttaa
      541 ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag ttaaataatt
      601 cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct acgattacgg
      661 atttattaga aaaagcattg aa
//
LOCUS       DAJVRL010000290          647 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVRL010000290 DAJVRL010000000
VERSION     DAJVRL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.300"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..492
                     /locus_tag="OQN02_004137"
     CDS             <1..492
                     /locus_tag="OQN02_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HCS1644714.1"
                     /translation="IKRDVGKIFVMNIEWAFANFVGAPGAVCHHQPTCGRSVIVEHNG
                     DVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQLPAQCRSCNVLKACWG
                     GCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLLAHGRPASDIMQAHLMV
                     VNK"
BASE COUNT          150 a          149 c          183 g          165 t
ORIGIN      
        1 atcaaacgcg acgtcggcaa gattttcgtg atgaatatcg aatgggcgtt tgccaatttt
       61 gtcggtgcgc cgggtgcggt ttgccatcat cagccaacct gtgggcgctc ggtgattgtt
      121 gagcacaacg gcgacgttta cgcctgcgat cactatgttt atccgcaata tcggctgggg
      181 aatatgcatc agcaaacaat tgcagaaatg atcgattccc cgcaacagca ggtgtttggt
      241 gaagataaat ttaagcaatt accggcgcag tgtcgcagtt gtaacgtgtt aaaagcatgt
      301 tggggaggct gcccgaaaca ccgcttcatg ctcgatgcca gcggcaaacc ggggctgaat
      361 tatttgtgtg ccgggtatca gcgttatttc cgccatctac cgccatatct taaagcaatg
      421 gctgatttgc tggcgcacgg tcgcccggcc agtgacatta tgcaggcaca tttgatggtg
      481 gtgaataagt aaaaaagcgg ccatttggaa gccgcctgcg gttgattgcc ggatgcggcg
      541 taaacgcctt atccggccta catgatcgtg caaattcaat aaattgcagc gttctgtgga
      601 tactggtttg ggtgatcgta tccggcaaag cctttacgtg cggtggc
//
LOCUS       DAJVRL010000291          632 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVRL010000291 DAJVRL010000000
VERSION     DAJVRL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.301"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>632
                     /locus_tag="OQN02_004138"
     CDS             <1..>632
                     /locus_tag="OQN02_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1644715.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLT"
BASE COUNT          205 a          121 c          154 g          152 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac at
//
LOCUS       DAJVRL010000292          619 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVRL010000292 DAJVRL010000000
VERSION     DAJVRL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.302"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..>619)
                     /locus_tag="OQN02_004139"
     rRNA            complement(<1..>619)
                     /locus_tag="OQN02_004139"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          131 a          188 c          146 g          154 t
ORIGIN      
        1 gtgatccaac cgcaggttcc cctacggtta ccttgttacg acttcacccc agtcatgaat
       61 cacaaagtgg taagcgccct cccgaaggtt aagctaccta cttcttttgc aacccactcc
      121 catggtgtga cgggcggtgt gtacaaggcc cgggaacgta ttcaccgtgg cattctgatc
      181 cacgattact agcgattccg acttcatgga gtcgagttgc agactccaat ccggactacg
      241 acgcacttta tgaggtccgc ttgctctcgc gaggtcgctt ctctttgtat gcgccattgt
      301 agcacgtgtg tagccctggt cgtaagggcc atgatgactt gacgtcatcc ccaccttcct
      361 ccagtttatc actggcagtc tcctttgagt tcccggccgg accgctggca acaaaggata
      421 agggttgcgc tcgttgcggg acttaaccca acatttcaca acacgagctg acgacagcca
      481 tgcagcacct gtctcacggt tcccgaaggc acattctcat ctctgaaaac ttccgtggat
      541 gtcaagacca ggtaaggttc ttcgcgttgc atcgaattaa accacatgct ccaccgcttg
      601 tgcgggcccc cgtcaattc
//
LOCUS       DAJVRL010000293          612 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVRL010000293 DAJVRL010000000
VERSION     DAJVRL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.303"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..474)
                     /locus_tag="OQN02_004140"
     CDS             complement(<1..474)
                     /locus_tag="OQN02_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCS1644716.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLA"
BASE COUNT          176 a          150 c          127 g          159 t
ORIGIN      
        1 tgctaatgta taggcaaatg catcccataa cgaagatgtt tggctatttt tactttgcgg
       61 caaaatcaca cttagctcat taaataaggt ttttatgact tcttttccct gccccgcggc
      121 aattaccgga caagaatgcg gttgcagtaa ctgattttcc tcagccccct gaaactgaaa
      181 gccgtacagc gcacaggtac acgtcgttgc cggatcgtta acgtcagagg ccaccgcatg
      241 cagcctgccg cgttctatca ccacaatgtc atccgcatgt gcgacatata acgaagaatc
      301 gatggtaaac cttgcaaccc ctttcttgac gtaaatcagc tcggtttcat tgtcatgaac
      361 gtgatgtcca gactcccatt ttggatcatc gctaaatgca aaacgtgaaa gccgtggcgt
      421 tttaccggcg acaaacagcg tttcactggc attatcaaaa cagcgttgat acatgacaac
      481 ctccctattc catgagcaag caaaaacaat atatgccgga taaaatacgg cgtgtattgc
      541 ggttatacaa ccgcgtttag cgagagctaa acaagattat ttacactgaa tgcaagattg
      601 tacggtcatg ga
//
LOCUS       DAJVRL010000294          602 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVRL010000294 DAJVRL010000000
VERSION     DAJVRL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.304"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="OQN02_004141"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="OQN02_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1644717.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
BASE COUNT          202 a          135 c          119 g          146 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tg
//
LOCUS       DAJVRL010000295          584 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVRL010000295 DAJVRL010000000
VERSION     DAJVRL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.305"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>584
                     /locus_tag="OQN02_004142"
     CDS             <1..>584
                     /locus_tag="OQN02_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1644718.1"
                     /translation="LEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKM
                     TNRRHEENWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLD
                     KAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQ
                     IDMQRRVAWINPEESKSNRAIGVALNDTACRVLK"
BASE COUNT          204 a          126 c          141 g          113 t
ORIGIN      
        1 gctcgaagag aaagcacata agaaatcact ggacgatgac aaaagccgga tcggattctg
       61 gcttcaacat ttcgcaggga tgcaactaag agacatcact gaatcaaaaa tttattcagc
      121 aatgcagaaa atgacgaacc ggcgtcatga ggaaaactgg aaactcaggg cagaatcatg
      181 cagaaaaaaa gggaaacctg ttccagaata cacgccaaaa ccagcgtccg ttgcaacgaa
      241 ggctacgcat ctttcattta taaaggccct actaagagcc gcagagcgtg aatggaaaat
      301 gctggataag gcaccaatta ttaaagtgcc tcaaccaaag aataaacgga tccgctggct
      361 ggagccccat gaagcacaaa ggctgattga tgaatgtccg gagccattaa agtctgttgt
      421 tgaatttgca ctggcaacag gcttaagacg ctcgaacatc atcaaccttg aatggcaaca
      481 aatagatatg cagcgccggg tggcatggat aaacccggaa gagagtaaat caaaccgcgc
      541 aattggcgtt gcgctgaatg atactgcatg tcgcgtattg aaaa
//
LOCUS       DAJVRL010000296          583 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVRL010000296 DAJVRL010000000
VERSION     DAJVRL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.306"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(41..100)
                     /locus_tag="OQN02_004143"
     CDS             complement(41..100)
                     /locus_tag="OQN02_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1644719.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..583)
                     /locus_tag="OQN02_004144"
                     /pseudo
     CDS             complement(339..583)
                     /locus_tag="OQN02_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          137 a          155 c          140 g          151 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccctccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctaaataatc ttgataccgt caa
//
LOCUS       DAJVRL010000297          572 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVRL010000297 DAJVRL010000000
VERSION     DAJVRL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.307"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..521)
                     /locus_tag="OQN02_004145"
     CDS             complement(<1..521)
                     /locus_tag="OQN02_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1644720.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRS"
BASE COUNT          134 a          165 c          152 g          121 t
ORIGIN      
        1 attgagcgga ttttttccgc acgcaacccc agcgaacgac gtaataccgc ttccggtagc
       61 gcgtccgcca cctgattgcg gaccgcccac caggataatt cagctagaga taattcacgc
      121 tcctgcgtac cgcttattgc gtgaccgatg acgtcaatca tccatgctga caggttttga
      181 tgagcaagtt gctcgagtga ttcggatgtc tggtcacgca gctggttgtc gcagtgccag
      241 cacaatacca ttgcgccggt accataacgg tgaatgacgg tttcgctgtg atgataatcg
      301 ccgtgtggcc actggcagga tttaatatgg cgcaacagcc agtcagacaa tgcgccagca
      361 ccaccagcag cacgaatcac ccgtgcgtta ctgaaaaacg gcagcaatgt tttgtcttcc
      421 actagcggct ggcgaacggc aggaacgacc ccggacggca gattacgcat gcttttcggt
      481 tccggctcca ccagtacccg ggtattgtgg aataccggca tggattcacg accgggctta
      541 aggaccacca gcccgagttc cggaaccaga ac
//
LOCUS       DAJVRL010000298          566 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVRL010000298 DAJVRL010000000
VERSION     DAJVRL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.308"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..297
                     /locus_tag="OQN02_004146"
     CDS             <1..297
                     /locus_tag="OQN02_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCS1644721.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="OQN02_004147"
     CDS             complement(405..>566)
                     /locus_tag="OQN02_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644722.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAJVRL010000299          564 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVRL010000299 DAJVRL010000000
VERSION     DAJVRL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.309"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..94)
                     /locus_tag="OQN02_004148"
     CDS             complement(<1..94)
                     /locus_tag="OQN02_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1644723.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATR"
     gene            160..564
                     /locus_tag="OQN02_004149"
                     /pseudo
     CDS             160..564
                     /locus_tag="OQN02_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          149 a          124 c          134 g          157 t
ORIGIN      
        1 gacgtgttgc aacgctggca acagaataac cgcgatcaac aacctgtttg actgcttcag
       61 ttttaaactc ttcgggataa cgcttaccgc tcatgggcac ctctctttaa gccatcttaa
      121 atgactctga ggtgtctgtt aaacccgtgg cgattcagtg agcgcattct gaccgaccgt
      181 gaaaatggtg aaaaaggcga taattctcaa gatacctctt atgcccgtgt aggcgtgaaa
      241 ggtgaaactc agatcaaccc tgaaatgact ggttacggtc agtttgaact agacctggaa
      301 gcatcaaaca gacacaaccc tgaccaaact cgtttggcat atgctggttt gagttacaaa
      361 gattttggtt cattcgacta cagtcgtaat gtcggtgtcg cgtatgatgc agaagcattt
      421 actgatatgt ttgttgagtg gggcggtgac tcatgggctg gtactgacct gttcatgacc
      481 aaccgtacca acggtgttgc aacctatcgt aacacagatt tctttggcat ggttgaaggt
      541 ctaaattttg ctctgcaata tcag
//
LOCUS       DAJVRL010000300          537 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVRL010000300 DAJVRL010000000
VERSION     DAJVRL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.310"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>537
                     /locus_tag="OQN02_004150"
     CDS             <1..>537
                     /locus_tag="OQN02_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644724.1"
                     /translation="QEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICEN
                     VVRTINARMVDVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTEPSFGSEVSDYG
                     IIAHESQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGC
                     FRHDGENITVVLSRDGDSQ"
BASE COUNT          120 a          141 c          144 g          132 t
ORIGIN      
        1 caggagattg tcgtcgaacc gattgaacag gctattctca tctctgagca gactaagctg
       61 tgggacgcca agtcatccac cggattcact cagggcacac ttcagcagga cgctatgaac
      121 atctgtgaga atgtggtccg taccatcaac gctcgcatgg tcgacgttct ggaatcctct
      181 aagctgctga agaccgtaga gcttcctgtc ctgactggta gcctgaccgc taaggcagac
      241 gctatcatgg acgctctgta tgagaacacc gagccttctt tcggttctga ggtgtctgac
      301 tacggtatta tcgcccatga gagccaactg aaagcactgt cccgtctggc tgctaaacag
      361 ggcttctctg gtgaagacgc tatcgtggat atgctgggta ccgacatcgc gtattacaac
      421 ggtgaggaca aaggtgtgtt catgttggct aaacgcttca cggccctgag cttcggctgc
      481 ttccgtcacg acggtgagaa tattacggtc gtcctttcac gtgatggcga ctctcag
//
LOCUS       DAJVRL010000301          535 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVRL010000301 DAJVRL010000000
VERSION     DAJVRL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.311"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
BASE COUNT          174 a           78 c          107 g          176 t
ORIGIN      
        1 ttatatgaat atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa
       61 atagccctgc tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg
      121 tatctgtcag cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag
      181 caatgtatct tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc
      241 ttacatcacg actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc
      301 acaccgtaat gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc
      361 tcttattttc cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga
      421 tctcatttcg agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg
      481 attggggttt tatgagaaat aaagagacgt attgtctttg tttaaacggt acgta
//
LOCUS       DAJVRL010000302          526 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVRL010000302 DAJVRL010000000
VERSION     DAJVRL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.312"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            2..>526
                     /gene="dcuC"
                     /locus_tag="OQN02_004151"
     CDS             2..>526
                     /gene="dcuC"
                     /locus_tag="OQN02_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1644725.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILE"
BASE COUNT          105 a          124 c          150 g          147 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaa
//
LOCUS       DAJVRL010000303          524 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVRL010000303 DAJVRL010000000
VERSION     DAJVRL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.313"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>524
                     /locus_tag="OQN02_004152"
     CDS             <1..>524
                     /locus_tag="OQN02_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1644726.1"
                     /translation="LEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAA
                     DIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLT
                     SMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKAL
                     KRALFLSAFAALRD"
BASE COUNT           99 a          176 c          143 g          106 t
ORIGIN      
        1 acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag aaaaactcgc
       61 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
      121 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      181 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      241 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      301 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      361 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      421 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      481 caaacgggcg ttgttcctgt cggccttcgc cgcgctcagg gatc
//
LOCUS       DAJVRL010000304          523 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVRL010000304 DAJVRL010000000
VERSION     DAJVRL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.314"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..121)
                     /gene="csgC"
                     /locus_tag="OQN02_004153"
     CDS             complement(<1..121)
                     /gene="csgC"
                     /locus_tag="OQN02_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS1644727.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCR"
     gene            complement(180..>523)
                     /gene="csgA"
                     /locus_tag="OQN02_004154"
     CDS             complement(180..>523)
                     /gene="csgA"
                     /locus_tag="OQN02_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCS1644728.1"
                     /translation="SGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVG
                     QGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQV
                     GFGNNATAHQY"
BASE COUNT          138 a          123 c          126 g          136 t
ORIGIN      
        1 ctctgcacag acaagattga gtaagagtaa cttcaggaat aatggtgtac atatcccctt
       61 gctgggtcgt attaaaggtt atctggctgg aaagtgccgc aaggagtaat aacgcattca
      121 tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca aatgatgtat
      181 taatactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt gacggaggag
      241 ttcgatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt aacagtcatt
      301 tcagaatttt tgccgttcca ctgatcaaga gtggcgctgt taccgaagcc acgttgggtc
      361 agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc accgccatgc
      421 tgggtaatag tcaagtcaga gttacgggca tcagtttgca gagcaagtgc agagttaccg
      481 ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc tat
//
LOCUS       DAJVRL010000305          523 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVRL010000305 DAJVRL010000000
VERSION     DAJVRL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.315"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>523
                     /locus_tag="OQN02_004155"
     CDS             <1..>523
                     /locus_tag="OQN02_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCS1644729.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYL"
BASE COUNT          141 a           89 c          139 g          154 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct ctt
//
LOCUS       DAJVRL010000306          512 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVRL010000306 DAJVRL010000000
VERSION     DAJVRL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.316"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(1..279)
                     /locus_tag="OQN02_004156"
     CDS             complement(1..279)
                     /locus_tag="OQN02_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644730.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OQN02_004157"
     CDS             220..405
                     /locus_tag="OQN02_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644731.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..>512)
                     /gene="hokD"
                     /locus_tag="OQN02_004158"
     CDS             complement(447..>512)
                     /gene="hokD"
                     /locus_tag="OQN02_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1644732.1"
                     /translation="EVRIRTGQTEVAVFTAYEPEE"
BASE COUNT          106 a          129 c          155 g          122 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tc
//
LOCUS       DAJVRL010000307          505 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVRL010000307 DAJVRL010000000
VERSION     DAJVRL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.317"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            87..>505
                     /locus_tag="OQN02_004159"
     CDS             87..>505
                     /locus_tag="OQN02_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCS1644733.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQV"
BASE COUNT          131 a          119 c          151 g          104 t
ORIGIN      
        1 gtggtgctga ccaacgctga attcacaggt gccggtgatg ctgacggcaa tgcagaaatt
       61 tcctggaaga tttaaggaac agacgaatga ttacttttga tcaggcaacc gttgacagct
      121 ctggtgcctt tctcatcggg gagctggaga gactcgacca gacgctgaac ctgccactgg
      181 tggggtacac ctggagccgc gatattcaac tgcgtgaaga cgcctccatc gcagatgaca
      241 tttccagctg gacgaatacc agcttcgccg ctgcgggtac tggcgcaaat ccgaatggaa
      301 aaaactgggt aggcaaagac tcaaccgcta ttgctggcgt gaacgtggat atcggcaaat
      361 ccggtaaccc gctgaacctg tgggggatgg aacttggctg gacggtcata gaattgcagg
      421 ctgctcagca ggtcggccgc ccgattgata cgcagaagta tgacggtatg caactgaaat
      481 ggcagatgga taacgatgaa caggt
//
LOCUS       DAJVRL010000308          500 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVRL010000308 DAJVRL010000000
VERSION     DAJVRL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.318"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            14..496
                     /locus_tag="OQN02_004160"
     CDS             14..496
                     /locus_tag="OQN02_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1644734.1"
                     /translation="MAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKELRVAL
                     NYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPSTPVSCQ
                     KSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVERLMQL
                     "
BASE COUNT          138 a          112 c          124 g          126 t
ORIGIN      
        1 taccaccgaa cttttggcgc agctttacgg cactgagccg gtgcgtattc gccagaatca
       61 tcatgagaac aaagtacgct tcgttgaagg gaaacacttt ttcaaagttg ttggtaatga
      121 ccttaaagaa ttgcgggtcg ctttaaacta ctcacaaaat ccagtttcac ccaaagcccg
      181 ctccctcatc ctctggacag aacgaggcgc agcacgccac ggcaaaatgc tcgaaaccga
      241 tcaggcgtgg gatgtgttcg aaaaactgga agactgctat ttcagtcaaa gggatccgtc
      301 aacgccagtt tcatgccaga aaagttacga cacgcgagtt ctctgttatc agagaggcgg
      361 tgtcactgtt tccacaattc agttacggga tgatgatatt gttatttccc ttgagtcatg
      421 gctggaactg gcgagagcca atggttggtt tgttgttcgc agagataaac tggtggaaag
      481 gttgatgcag ctttaaaaaa
//
LOCUS       DAJVRL010000309          495 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVRL010000309 DAJVRL010000000
VERSION     DAJVRL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.319"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..>495)
                     /locus_tag="OQN02_004161"
     rRNA            complement(<1..>495)
                     /locus_tag="OQN02_004161"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           92 a          165 c          109 g          129 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcag
//
LOCUS       DAJVRL010000310          492 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVRL010000310 DAJVRL010000000
VERSION     DAJVRL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.320"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..177
                     /locus_tag="OQN02_004162"
     CDS             <1..177
                     /locus_tag="OQN02_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1644735.1"
                     /translation="ADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            174..>492
                     /locus_tag="OQN02_004163"
     CDS             174..>492
                     /locus_tag="OQN02_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1644736.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          144 a          124 c          133 g           91 t
ORIGIN      
        1 gccgatcgtg acgggttatg cctgttcacc aaacgtctgg ccggtgaccc gggaagggaa
       61 agtgcacctg acgccagctc agttatccat gctactggag gggatcgcgt ggcaacatcc
      121 caaacggaca gaacggcctg gcatccggat ataacccgtg ataaaacaag ggaatgaaca
      181 atgaactccc cgatgatatt gagctgctta aagccatgtt gcgtaagcaa cagagtcggc
      241 ttcgacagta tgcctgtcag gtcgcgggct atgagcagga aattgaacgg ctgaaagcgc
      301 aactcgacag gttgcgtcgt atgttgttcg gccagagttc agagaaaaag cgtcataagc
      361 ttgaaaatca gatccgacag gcagaaaaac gactgtcgga actggaaaac cggctgaaca
      421 cagccagaaa tcttctggaa gatgcatcgt cagtcacaga ttcacctgac accagtcccc
      481 cgtcagaaaa cc
//
LOCUS       DAJVRL010000311          487 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVRL010000311 DAJVRL010000000
VERSION     DAJVRL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.321"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            209..>487
                     /locus_tag="OQN02_004164"
     CDS             209..>487
                     /locus_tag="OQN02_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1644737.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAM"
BASE COUNT          110 a          128 c          133 g          116 t
ORIGIN      
        1 ttataatggg gtgttagcca gccagacccg gcatgattac tgcccccagt cgtccatgat
       61 ccgggggggg atgtcaccgg gtctggtggg gcgctggtaa ccgctaatag gggtcaggtc
      121 aggcactttt gccgggaccg tctgtaacgt ggatgccggt acctgctccc cgtggttatc
      181 tggttaaccc atatacaagg gagacagaat gaccgaatcc agcgattacg aatccgtcca
      241 ggtctttatc ggcgttgatg tcggtaaaga tacgcatcac gctgttgcca ttaatcgttc
      301 aggtaaacgc ctgttcgata aagcattacc caacgacgaa aacaaactca ggtcgctaat
      361 atctgacctg aaacaacatg gtcagatact gctggttgtt gatcagccag ctaccatcgg
      421 tgcgttacct gtcgccgttg cccgctcaga aggagtcctt gtcggatacc tccctggact
      481 ggccatg
//
LOCUS       DAJVRL010000312          486 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVRL010000312 DAJVRL010000000
VERSION     DAJVRL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.322"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..195
                     /locus_tag="OQN02_004165"
                     /pseudo
     CDS             1..195
                     /locus_tag="OQN02_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            295..>486
                     /locus_tag="OQN02_004166"
     CDS             295..>486
                     /locus_tag="OQN02_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644738.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDL"
BASE COUNT          169 a           99 c           95 g          123 t
ORIGIN      
        1 ctacgtaatt aaaacagaaa ataaagaaac cccattcact gtagttgata tcgacggtct
       61 atcaggaaac tttaaaactc ttgttgaagg tgccacaaaa atgtgcctag tttacatcgg
      121 aaataatctg cccgcagaaa aaaagccgaa tttctggcaa atctgattgc aatgaaatta
      181 aaaggtgaaa tctgaaaaaa agaaagcctg cacagtgtgc aggcctgagt gaagaacctg
      241 ggacatttat tcatcactcg cagtaatttt aatctgagtt gaggttaaaa aacaatgagc
      301 acaaaaccac tcttcctgtt acggaaagcg aaaaaatcat ccggtgaacc tgacgtcgtc
      361 ctgtgggcaa gcgacgattt tgaatcgacc agcaccactc tggactatct gctcgttaag
      421 tcaggtaaaa aactgagcaa ctattttaaa gctgttgcca caaattttcc tgtcgttaat
      481 gacctt
//
LOCUS       DAJVRL010000313          484 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVRL010000313 DAJVRL010000000
VERSION     DAJVRL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.323"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>484
                     /locus_tag="OQN02_004167"
     CDS             <1..>484
                     /locus_tag="OQN02_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1644739.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          109 a          107 c          123 g          145 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaa
//
LOCUS       DAJVRL010000314          480 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVRL010000314 DAJVRL010000000
VERSION     DAJVRL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.324"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..>480)
                     /locus_tag="OQN02_004168"
     CDS             complement(<1..>480)
                     /locus_tag="OQN02_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCS1644740.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA"
BASE COUNT          129 a          153 c          103 g           95 t
ORIGIN      
        1 atgcgcaatg gaccagaacg ctgccttgct cttcgcgtaa tgtttccagc atcgccacgg
       61 cctgtcggag ctccccctct tccggaacca ccagatccaa catcggtaca cagaaataga
      121 gtcgatcttt tgtcgctcgt ccacgaggga attcaaaggt gacgtccaga accgcattct
      181 gtgccggaat atgtcgtgga aacgccctta aataaacacc agcagtcatt ttgctcaccg
      241 gctccaggcg acgacaaaac caacgcatag atagccacat cccgatgcgc cagggcaatg
      301 tcagccagta aacggcgggc gggagtttcc cctgactatc tttccctgtt gttatcgcgc
      361 caagcccacc gcacccacgg ccaataatga gtagcgataa tactggccaa cataaccaga
      421 ctgaccacca taactgaacc atcatcatta gctccatcaa cacaatgcac gcgcctacga
//
LOCUS       DAJVRL010000315          480 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVRL010000315 DAJVRL010000000
VERSION     DAJVRL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.325"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(52..480)
                     /locus_tag="OQN02_004169"
                     /pseudo
     CDS             complement(52..480)
                     /locus_tag="OQN02_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
BASE COUNT           99 a          122 c          124 g          135 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc cgatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatcctg
      301 gcaatcctcg tgggcgtctg cctgcgcgtt ttcttcctgg cctcccgcca gttctttggt
      361 ttcttgagca ttatcctggt ggtgaacgtc gtctggcgct gctcccggtt ttagttccca
      421 tgtcatggag tctttgctga gttgatagcg ttcactccag gtaaaatcga tctcaccttc
//
LOCUS       DAJVRL010000316          468 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVRL010000316 DAJVRL010000000
VERSION     DAJVRL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.326"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..221)
                     /locus_tag="OQN02_004170"
     CDS             complement(<1..221)
                     /locus_tag="OQN02_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644741.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTT"
     gene            complement(225..>468)
                     /locus_tag="OQN02_004171"
     CDS             complement(225..>468)
                     /locus_tag="OQN02_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644742.1"
                     /translation="FKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKG
                     RNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          104 a          126 c          123 g          115 t
ORIGIN      
        1 gtggtcggat aagggcgcac gtagatcccc ttcaccttgt ctgcggtatc accatctgcc
       61 agcggcacga aaaaaccgtc atcatcgtat ttaccagcca ggccataggc agcgaaggcg
      121 ttatcggatt taaggaccac cggttcgacg gttaagtcct gcgggcgaga gacagccccg
      181 gcaatgccaa caggcatccg gtacagaaat acattattca ttttttaccc tttacggttt
      241 gcccagaatt cagcgttttg tttgttcagg gaagcgatac tggtcatgcc catgtttagg
      301 cgctgtgcat cgccggtggt ggcgcgggtg tttcgccctt tggcaacctc agacacggca
      361 ttaaacgcca tgtcgaccga ttgtttcggt aatttgcgga tatccgcatc accgactatc
      421 tggcgaacca gcgttttgtc agcagaagcc agaacctcgc gtttgaac
//
LOCUS       DAJVRL010000317          465 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVRL010000317 DAJVRL010000000
VERSION     DAJVRL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.327"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..329
                     /locus_tag="OQN02_004172"
     CDS             <1..329
                     /locus_tag="OQN02_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCS1644743.1"
                     /translation="FALLSSIIVSSAGNQSLLTNLQTNTISYHQNGVPLNIRAVKWLK
                     GRGVGNKDRMVAYTNDKKYVRYPLVPLQSVPVQYRGLYQIVTYYGKLGAVEPVYKETI
                     SYVDGI"
BASE COUNT          111 a          123 c          120 g          111 t
ORIGIN      
        1 agtttgctct gttgtccagc atcatcgttt catctgcagg taaccagtcc ctgttgacga
       61 accttcagac caacaccatc agctatcacc agaacggtgt tccgctgaat atccgcgcgg
      121 ttaaatggct gaaaggccgt ggtgtgggga ataaggatcg catggttgcg tacaccaacg
      181 ataaaaaata cgtccgctac ccgctggttc cgcttcagag cgtgccggtg cagtatcgcg
      241 gtctgtatca gatcgtcact tactacggca agctgggtgc agtcgagcca gtgtacaaag
      301 aaaccatttc gtacgttgat ggcatttaac agccacatgg ccccctggcg gggccattaa
      361 ggatgacccg atggcaaaaa atgatgcagt aatacacgta cataccccgt ttgtgctcac
      421 gcttcccgac ggttctcggc gcgagtttgt taaaggccgt catgt
//
LOCUS       DAJVRL010000318          447 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVRL010000318 DAJVRL010000000
VERSION     DAJVRL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.328"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>447
                     /locus_tag="OQN02_004173"
     CDS             <1..>447
                     /locus_tag="OQN02_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCS1644744.1"
                     /translation="RQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRT
                     PDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLI
                     EQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEEL"
BASE COUNT          113 a          131 c          126 g           77 t
ORIGIN      
        1 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
       61 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      121 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      181 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      241 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      301 acttatagaa caaaccggcg ataacactct gacgttaatg gataaaggtt attactcact
      361 gggactgtta aatggctgga gcctggcggg agaacaccgc cactggatga tacctctcag
      421 aaagggagcg caatatgaag agctcag
//
LOCUS       DAJVRL010000319          420 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVRL010000319 DAJVRL010000000
VERSION     DAJVRL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.329"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..420
                     /locus_tag="OQN02_004174"
                     /pseudo
     CDS             1..420
                     /locus_tag="OQN02_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          102 a          116 c          124 g           78 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
//
LOCUS       DAJVRL010000320          417 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVRL010000320 DAJVRL010000000
VERSION     DAJVRL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.330"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
BASE COUNT          130 a           97 c          100 g           90 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatat
//
LOCUS       DAJVRL010000321          415 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVRL010000321 DAJVRL010000000
VERSION     DAJVRL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.331"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            28..300
                     /locus_tag="OQN02_004175"
     CDS             28..300
                     /locus_tag="OQN02_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644745.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
BASE COUNT          136 a           84 c          104 g           91 t
ORIGIN      
        1 aaagtaaatg aaacagatgc tgaatacctg gtgcgtaaat tcgatgagct tgaagctaaa
       61 tgtgcagcac aggaaaacaa agtaatacca gtgtcaactg aactgccacc agcaaatgaa
      121 agtgttttgt tattcgatgc taacggagaa ggctggctaa ttggctggcg ttctctctgg
      181 tacacctggg gacaaaaaga aaccggagaa tggcagtgga catttcaggt cggggacctt
      241 gaaaacgtca atatcactca ctgggcagta atgccaaaag caccggaggc tggagcataa
      301 tgaccacttt taccgacaaa gaactgatta aagaaattaa agagcgtatc agcagccttg
      361 acgtgcgaga cgatattgag cgccgtgctt atgaaatcgc actcctatct ctgga
//
LOCUS       DAJVRL010000322          407 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVRL010000322 DAJVRL010000000
VERSION     DAJVRL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.332"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
BASE COUNT          123 a           81 c           61 g          142 t
ORIGIN      
        1 aacaggatgc tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta
       61 ccctcatcgg taaaaaaatt gcctttcgac cattacgata cattattgat tttggggttt
      121 cagggaagta atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt
      181 tgctactaaa tagtttcttt attttttcca cgatgccccc aggtttataa gtacaaacgg
      241 ttatatccac atagagacaa aaatattaat ctgataaata tttatttcac gccgtatatt
      301 tgattgttta atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat
      361 atggttattt ttcaccctgc gtattcagca cgcaacaaaa aacccgc
//
LOCUS       DAJVRL010000323          407 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVRL010000323 DAJVRL010000000
VERSION     DAJVRL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.333"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..116
                     /locus_tag="OQN02_004176"
     CDS             <1..116
                     /locus_tag="OQN02_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCS1644746.1"
                     /translation="QWAAIGVLGSLLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            116..>407
                     /locus_tag="OQN02_004177"
     CDS             116..>407
                     /locus_tag="OQN02_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCS1644747.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPET"
BASE COUNT          117 a           83 c          106 g          101 t
ORIGIN      
        1 cacagtgggc tgcgattggt gtgctgggaa gtctgcttct ggggcttctg acttatctga
       61 cgaatctgta tttcaaaata agagaagata agcgtaaggc tgcgagaggt gaataatgtc
      121 gccatcatta cgcaaggctg ttgcagttgc tattggtggc ggggctgttg ctatagcatc
      181 tgtgttaatc actggcccag gtggtaacga tggtctggaa ggtgtcagct acataccata
      241 caaagatatc attggtgtat ggactgtatg tcacggacac accggaaaag acatcatgcc
      301 tggtaaaacg tataccgaag cagaatgcaa agctctcctg aataaagacc ttgtcactgt
      361 cgccagacaa attaacccgt acatcaaagt cgatataccg gaaacaa
//
LOCUS       DAJVRL010000324          404 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVRL010000324 DAJVRL010000000
VERSION     DAJVRL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.334"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>404
                     /locus_tag="OQN02_004178"
     CDS             <1..>404
                     /locus_tag="OQN02_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1644748.1"
                     /translation="VRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNAL
                     NEFCRDGWVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVE
                     PEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT          115 a           85 c          121 g           83 t
ORIGIN      
        1 cagtcagaaa agccagaagc gtccagttga tgcagtcgtt gtacgactgg atacagttgc
       61 agaggaaaac gctgtcgaaa catgcggaga tggcgaaggc gttcgactat atcctgaatc
      121 actggaatgc gctgaacgag ttctgtcgtg acggctgggt ggaaatagac aacaacatcg
      181 gtgaaaacgc gttacgatcg gtggcggttg gaagaaaaaa ttatctcttt ttcggctcag
      241 acaagggagg agaaagtgcg gcgatcatct acagtctgct ggtcacctgc aaacagaacg
      301 aagtggagcc ggaggactgg ttgcgcgaag tgatcgagaa gctcaatgac tggccgtcga
      361 accaagtgca tgaactgctg ccctggaact tctcgtctgt aaaa
//
LOCUS       DAJVRL010000325          398 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVRL010000325 DAJVRL010000000
VERSION     DAJVRL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.335"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            1..333
                     /locus_tag="OQN02_004179"
                     /pseudo
     CDS             1..333
                     /locus_tag="OQN02_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          142 a           86 c           93 g           77 t
ORIGIN      
        1 attatttgcc aacgacggta caccgacatg gctaaaagaa aagcgtgcaa aagaaatcgt
       61 acttgatgaa attaacttga tgaaattatc gagttgctca acaagcacca tgggggcacc
      121 agtaacgacg ccaaacgcgc aaaagctgat ttgctggaaa aaacattcac ctcccgctca
      181 tgggaaagaa tcaaaggtat ggactggccg acaatcaaag ctggtcgtaa tgcactatgg
      241 attgaactgg aaggcgttga atatgcattc ccggatccac aaacacaaaa cgaaacgcaa
      301 caagcaggat acgatgagaa tattccggtg taaaaccaag tgcattttgg gaatgaaccg
      361 ccccgggaat cctggagact aaacttcctg agaaagag
//
LOCUS       DAJVRL010000326          393 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVRL010000326 DAJVRL010000000
VERSION     DAJVRL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.336"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>393
                     /locus_tag="OQN02_004180"
     CDS             <1..>393
                     /locus_tag="OQN02_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644749.1"
                     /translation="CEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLE
                     ILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTA
                     EAMVRSREENEFTDWFSLWGPWHAVLKR"
BASE COUNT           94 a          103 c          106 g           90 t
ORIGIN      
        1 gctgtgagga ccgtgtcgcg ctcacatgga acaatctccg gaaaaccctc ctggtccatc
       61 aggcatcaga aggccttttc gataatgata ccggcgctct gctctccctg ggcagggaaa
      121 tgttccgcct cgaaattctg gaggatattg cccgggataa agtcagaact ctccattttg
      181 tggatgagat agaagtctac ctggccttcc agaccatgct cgcagagaaa cttcagctct
      241 ccactgccgt gaaggaaatg cgtttctatg gcgtgtcggg agtgacagca aatgacctcc
      301 gcactgccga agccatggtc agaagccgtg aagagaatga atttacggac tggttctccc
      361 tctggggacc atggcatgct gtactgaagc gta
//
LOCUS       DAJVRL010000327          388 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVRL010000327 DAJVRL010000000
VERSION     DAJVRL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.337"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>388
                     /locus_tag="OQN02_004181"
     CDS             <1..>388
                     /locus_tag="OQN02_004181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644750.1"
                     /translation="KIGFKNDSDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMN
                     LKWDSLNILVNAVINGCLGIGTTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVF
                     RFQNGVAIAFKNIQAGTARKFTLSSA"
BASE COUNT          109 a           74 c          100 g          105 t
ORIGIN      
        1 cgaagattgg atttaaaaat gattcggatg cagacaccga ttcctacatg tggtttgaaa
       61 caggtgacaa cggcaatgag tatttcaaat ggagacaccg cctcgctggc ggccagctca
      121 aagagctgat gaatcttaaa tgggattcac taaatattct ggttaatgcc gtcattaatg
      181 gttgccttgg cattggtacg acgaatgcgt taggtggaaa ttcaattgct ttcggggata
      241 acgataccgg cctgaaacag aacggcgatg gtctgctgga tgtttatgcg aatggacagc
      301 atgtatttcg ttttcagaat ggcgtggcta ttgcttttaa gaatattcag gccggaactg
      361 ccagaaaatt cacgttatcc agcgccaa
//
LOCUS       DAJVRL010000328          383 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVRL010000328 DAJVRL010000000
VERSION     DAJVRL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.338"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(168..>383)
                     /locus_tag="OQN02_004182"
     CDS             complement(168..>383)
                     /locus_tag="OQN02_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1644751.1"
                     /translation="LEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMR
                     FTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT           91 a          122 c           75 g           95 t
ORIGIN      
        1 aaaaagatta aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctccatat
       61 gaacggaacc gacgattaaa ctggatggct ctgattgatt cagggtatga atggcggttt
      121 tttgctccgt ttccctcaaa atggacgcaa cttcccctct gcggctctca gccgcaccac
      181 cgcatccggg ccagcggctc atgcatcagg acctgctctg ccagacggta gccccgcttc
      241 agccccgtaa aacgcatctg actcccgcac agcacgcact tcagcgggtc aaccttcagt
      301 aacctctgat acatccctct ccaggtgatt tgcatcgccg tttttctcac tgtctccgtt
      361 atgatgtaca ccacttcttc cag
//
LOCUS       DAJVRL010000329          352 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVRL010000329 DAJVRL010000000
VERSION     DAJVRL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.339"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(9..>352)
                     /locus_tag="OQN02_004183"
     CDS             complement(9..>352)
                     /locus_tag="OQN02_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644752.1"
                     /translation="GDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEG
                     VQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKL
                     INGTWYNVASI"
BASE COUNT           88 a           94 c           73 g           97 t
ORIGIN      
        1 caacattgtt aaatactcgc tacgttatac caggtgccat taatcagttt ttgcagtggt
       61 cggtaataca caccgtcaac gttatccgct gagttacggc cggtatccga tatcgccatc
      121 cctgacagcc cgtgtcccga aggtgcgcaa aatctccacg aaacggtgtt actgccgggg
      181 ctgtaataca tttcatggcc ataccgtaca tcctgtaccc cctctgttcg atatttatag
      241 cgggcatcga agtttccgta atttaacgga attacctgtc cgttaacagt gaacactatg
      301 ctgttatccg ggtttctctg gctgaaaaaa tgccagcctg aatcatcacc aa
//
LOCUS       DAJVRL010000330          352 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVRL010000330 DAJVRL010000000
VERSION     DAJVRL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.340"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
BASE COUNT          101 a           87 c           64 g          100 t
ORIGIN      
        1 tcagcaaaac tctgatgacc tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg
       61 actaattgac aataattttc atttccatta aaaaagatcg cgtagcattc ttaattcatg
      121 aggaacttta cccgccagca atctgagtag cgaaagctgt tcatccccaa tgtttttgct
      181 ggcgggtcct tttttctacg gtcctcctcc ccggaggggg gcatttcatt atttttctaa
      241 tcgttactgg tgagaaggcg gaagaaaaag ccagttcttc gcccctaaat aacagcatga
      301 ttccagtcca tgcaaaatct gatttccagc tcacacatac cgagcgctgt ac
//
LOCUS       DAJVRL010000331          351 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVRL010000331 DAJVRL010000000
VERSION     DAJVRL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.341"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(43..330)
                     /locus_tag="OQN02_004184"
     CDS             complement(43..330)
                     /locus_tag="OQN02_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644753.1"
                     /translation="MDEEYRKDLQLWFGLTHTSFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           94 a           88 c           69 g          100 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgta tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgtctttacc tttgttgcaa t
//
LOCUS       DAJVRL010000332          344 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVRL010000332 DAJVRL010000000
VERSION     DAJVRL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.342"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OQN02_004185"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OQN02_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1644754.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJVRL010000333          338 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVRL010000333 DAJVRL010000000
VERSION     DAJVRL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.343"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            179..>338
                     /locus_tag="OQN02_004186"
     CDS             179..>338
                     /locus_tag="OQN02_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1644755.1"
                     /translation="MRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDNL
                     KKINGKNEK"
BASE COUNT          117 a           67 c           82 g           72 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagt
//
LOCUS       DAJVRL010000334          326 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVRL010000334 DAJVRL010000000
VERSION     DAJVRL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.344"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>326
                     /locus_tag="OQN02_004187"
     CDS             <1..>326
                     /locus_tag="OQN02_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1644756.1"
                     /translation="QYQMTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGDGRVV
                     WVGKDVTGIEPRNASVIEVPDITTNRRITAPGYWFYRNDEFVFDYRLKAEDERDALLA
                     QVSART"
BASE COUNT           94 a           72 c           86 g           74 t
ORIGIN      
        1 aacaatatca gatgacaaaa cgatattcgg tcacctggct tttttcagaa gacggaaaaa
       61 actggtatga agagctgaag aatttcgcca gcgacacaat aaaaatagct tacaccggag
      121 atggtcgcgt ggtgtgggtc ggtaaggatg tgacaggcat cgagccacgc aatgccagcg
      181 ttatcgaagt tccggatatt accactaacc gacggattac cgcgccgggt tactggtttt
      241 accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc
      301 ttctggctca ggtcagtgcc cggaca
//
LOCUS       DAJVRL010000335          320 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVRL010000335 DAJVRL010000000
VERSION     DAJVRL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.345"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..>320)
                     /locus_tag="OQN02_004188"
     CDS             complement(<1..>320)
                     /locus_tag="OQN02_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HCS1644757.1"
                     /translation="VIVCAEMDEQWGYVGAKSRQRWLFYAYDRPRKTVVAHVFGERTM
                     ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLAR
                     LGRKS"
BASE COUNT           65 a          108 c           79 g           68 t
ORIGIN      
        1 gacttccgtc ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc
       61 tgcgtatatc gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc
      121 cagccatccg tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc
      181 agcgtcgcca tcgtgcgttc accgaagacg tgcgcaacaa ccgtcttccg gggcctgtca
      241 tacgcgtaaa acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc
      301 atttccgcgc agacgatgac
//
LOCUS       DAJVRL010000336          317 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVRL010000336 DAJVRL010000000
VERSION     DAJVRL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.346"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(31..89)
                     /locus_tag="OQN02_004189"
                     /pseudo
     CDS             complement(31..89)
                     /locus_tag="OQN02_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           69 a           95 c           72 g           81 t
ORIGIN      
        1 gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta cgagtaacaa
       61 ccaaccagta tgatgacgaa cttcatcata accctttcct tctataaggc cccttcctcg
      121 ggaggggctt tcccgtttca gccccttgct gactccccga ttgttgatga gcgccggacc
      181 ccgcgcaacg ctttccctga agcagttttg ccttccccga gcggtcacgc aaaacgctgc
      241 aacgtcctga gcatttgcac cttttagcgg aacgcagata taaaaaatcc ccggcttttt
      301 gggccaggga tatgtgt
//
LOCUS       DAJVRL010000337          310 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVRL010000337 DAJVRL010000000
VERSION     DAJVRL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.347"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>310
                     /locus_tag="OQN02_004190"
     CDS             <1..>310
                     /locus_tag="OQN02_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCS1644758.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     T"
BASE COUNT           90 a           75 c           65 g           80 t
ORIGIN      
        1 aaaaccgaca gaatccattt gaaagacgca gcgcgaatgc tggaagtttc tgtaatgact
       61 attcgtcgcg atctccatca ggaagatgaa cctctgccac tgaccctact gggtggctat
      121 attgtaatgg tgaataaacc cgcgccatcc atgccagtaa tccatgacgt tccaaaaaat
      181 catcgtgatg acttacctat tgcaattctg gctgccggaa tggttaatga aaatgatctg
      241 atcttctttg ataatggcca ggagatacca ctcgttataa gcatgatccc ggatgcaatc
      301 accttcaccg
//
LOCUS       DAJVRL010000338          305 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVRL010000338 DAJVRL010000000
VERSION     DAJVRL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.348"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..>305
                     /locus_tag="OQN02_004191"
     CDS             <1..>305
                     /locus_tag="OQN02_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1644759.1"
                     /translation="LSATYTIDGFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYD
                     ANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT           81 a           64 c           70 g           90 t
ORIGIN      
        1 gtttatctgc aacttacact attgacggtt tctcatttgc tggtgcttat gcaaactctg
       61 accgtactga ttggcagtct ggtgatggta aaggcgaaag agccgaagtt tgggcgttgt
      121 caacaaaata tgacgctaac aatgtttatg ctgctgtgat gtacggtgaa tctcacaaca
      181 tgaattccga cgacggtgat gttgtgaaca aaacacaaaa cttcgaagct gttctgcaat
      241 atcagttcga ctttggtctg cgtccatcca tcggttactc ctattctaaa gcactggacg
      301 tagca
//
LOCUS       DAJVRL010000339          299 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVRL010000339 DAJVRL010000000
VERSION     DAJVRL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.349"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..177)
                     /locus_tag="OQN02_004192"
     CDS             complement(<1..177)
                     /locus_tag="OQN02_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1644760.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
BASE COUNT           66 a           76 c           72 g           85 t
ORIGIN      
        1 cctgggtact ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa
       61 cgcaagcccc tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc
      121 aagactgctc atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc
      181 ggtatttcct ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc
      241 acctatacgg cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgc
//
LOCUS       DAJVRL010000340          297 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVRL010000340 DAJVRL010000000
VERSION     DAJVRL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.350"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..66)
                     /locus_tag="OQN02_004193"
     CDS             complement(<1..66)
                     /locus_tag="OQN02_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HCS1644761.1"
                     /translation="MVDLLKAARGQMCTVRIPGYCN"
     gene            complement(66..>297)
                     /locus_tag="OQN02_004194"
     CDS             complement(66..>297)
                     /locus_tag="OQN02_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1644762.1"
                     /translation="FREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDETEFQQVYK
                     SVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT           82 a           87 c           63 g           65 t
ORIGIN      
        1 attgcagtag ccaggaattc tgacggtgca catctgcccc cgcgccgctt taagtaaatc
       61 caccattacg caaactccag cagctgcgcg gccacatttt cgacttgttc cggagaggaa
      121 aatttacgga acagaatcca gttccacagc acattcagta cagatttata aacctgctga
      181 aactcggttt cgtccatatt cgcaaacgcg atagatttcg cccgacaccc acggctaccg
      241 tccggataaa aatgctcggt gtaaaatccg gcctgaatgg ctacccactc gcggaaa
//
LOCUS       DAJVRL010000341          295 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVRL010000341 DAJVRL010000000
VERSION     DAJVRL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.denovo.351"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            38..>295
                     /locus_tag="OQN02_004195"
     CDS             38..>295
                     /locus_tag="OQN02_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCS1644763.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQ"
BASE COUNT           64 a           81 c           88 g           62 t
ORIGIN      
        1 cggttcactg gccggacgga gtcgccccgc ccttctgatg ctcccccgtt ttgccgatat
       61 ttttcagcag ggaaaccgct ggcttaactg gctggagaaa caaccggaag gggcagtccg
      121 tccggtggta attgaatcag tgacaaaaat catggcctgc gggaccacac tgatgggcta
      181 cacgcagtgg tgttgttcat caccggactg ttgccataca aaaaaggtct gcttccggtg
      241 taaaagccgc tcctgcccgc actgcggggt gaaggctggc gcacagtgga tacag
//
LOCUS       DAJVRL010000342         6593 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.157, whole genome shotgun sequence.
ACCESSION   DAJVRL010000342 DAJVRL010000000
VERSION     DAJVRL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.157"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(16..204)
                     /locus_tag="OQN02_004196"
     CDS             complement(16..204)
                     /locus_tag="OQN02_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1644764.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="OQN02_004197"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="OQN02_004197"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1644765.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="OQN02_004198"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="OQN02_004198"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1644766.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="OQN02_004199"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="OQN02_004199"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1644767.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="OQN02_004200"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="OQN02_004200"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1644768.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="OQN02_004201"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="OQN02_004201"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1644769.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5099..6232
                     /locus_tag="OQN02_004202"
     CDS             5099..6232
                     /locus_tag="OQN02_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1644770.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..>6593
                     /gene="blc"
                     /locus_tag="OQN02_004203"
     CDS             6321..>6593
                     /gene="blc"
                     /locus_tag="OQN02_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1644771.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEG"
BASE COUNT         1674 a         1698 c         1699 g         1522 t
ORIGIN      
        1 gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accaactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg cagatccgtg
     2281 ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatcc
     2641 ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     6421 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     6541 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa ggg
//
LOCUS       DAJVRL010000343         4758 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.190, whole genome shotgun sequence.
ACCESSION   DAJVRL010000343 DAJVRL010000000
VERSION     DAJVRL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.190"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..359
                     /locus_tag="OQN02_004204"
     CDS             <1..359
                     /locus_tag="OQN02_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCS1644772.1"
                     /translation="LRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAVVLWNTVYLERAAHALRGNGHAVDDALLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            438..1442
                     /locus_tag="OQN02_004205"
     CDS             438..1442
                     /locus_tag="OQN02_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HCS1644773.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(1624..1800)
                     /locus_tag="OQN02_004206"
     CDS             complement(1624..1800)
                     /locus_tag="OQN02_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCS1644774.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            2130..2945
                     /gene="sul2"
                     /locus_tag="OQN02_004207"
     CDS             2130..2945
                     /gene="sul2"
                     /locus_tag="OQN02_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1644775.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3006..3809
                     /locus_tag="OQN02_004208"
                     /note="aph(3'')-Ib"
     CDS             3006..3809
                     /locus_tag="OQN02_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1644776.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3809..4645
                     /locus_tag="OQN02_004209"
     CDS             3809..4645
                     /locus_tag="OQN02_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1644777.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT         1044 a         1274 c         1305 g         1135 t
ORIGIN      
        1 aactgcgccg ccgcgtgcat gccggcctga acaagggcga ggcgcgcaat gcgctggcca
       61 gggcagtgtt tttcaaccgc ctgggtgaaa tccgcgaccg cagtttcgag cagcagcgct
      121 accgggctag cggcctcaat ctggtaacgg ctgccgtcgt gttgtggaac acggtctatc
      181 tggaacgggc tgcgcacgcg ctgcgtggca acggccatgc cgttgatgac gcgctgttgc
      241 agtacctgtc gccgctcggt tgggagcaca tcaacctcac cggcgattac ctctggcgca
      301 gcagcgccaa gatcggcgcg ggcaagttca ggccgctacg accgctgcaa ccggcttagc
      361 gtgctttatt taatgagatg gtcactccct ccttcccagt actatgctga ggacaggctt
      421 tcattcggag aaccatcatg gaaaacattg cgcttattgg tatcgatctg ggtaagaact
      481 ctttccatat tcattgtcag gatcatcgtg ggaaggccgt ttaccgtaaa aaattcaccc
      541 gaccaaagct aatcgaattt ctggcgacat gcccggcaac aaccatcgcg atggaagcct
      601 gtggcggttc tcactttatg gcacgcaagc tggaagagtt agggcatttt ccaaagctga
      661 tatcaccgca atttgtccgc ccattcgtta aaagcaacaa aaatgacttc gttgatgctg
      721 aagctatctg tgaagcagca tcacgtccat ctatgcgttt cgtgcagccc agaaccgaat
      781 ctcagcaggc aatgcgagct ctgcatcgtg tccgtgaatc cctggttcag gataaggtga
      841 aaacaactaa tcagatgcat gcttttctgc tggaatttgg tatcagcgtt ccgcgaggtg
      901 ctgccgttat tagtcgactg agtacccttc ttgaggacag tagtttgcct ctttatctca
      961 gccagttact gctgaaatta caacagcatt atcactatct tgttgagcag attaaagatc
     1021 tggaatctca gttgaaacga aagttggacg aagatgaggt tggacagcgc ttgctgagta
     1081 ttccctgcgt tggaacgctg actgccagta ctatttcaac tgagattggc gacgggaagc
     1141 agtacgccag cagccgtgac tttgcggcgg caacagggct ggtaccccga cagtacagca
     1201 cgggaggtcg gacgacattg ttagggatta gcaagcgggg caacaaaaag atccgaactt
     1261 tgttggttca gtgtgccagg gtattcatac aaaaactgga acaccagtct ggcaagttgg
     1321 ccgactgggt cagggagttg ttgtgtcgga aaagcaactt tgtcgtcacc tgtgctctgg
     1381 caaacaagct ggccagaata gcctgggcac tgacggcgcg acagcaaact tacgaagcat
     1441 aaaggcagaa atacaccagt ttaaacaatc attcatctgg ttttgcgaat actgatattg
     1501 atgatactaa cggcccaccg gcctgttgag gaacctgtaa aacggaaagg ctcattgaag
     1561 ccgtatattt tctggaggtt catcaggcgc ggaactcatc gaggcgcggg aataaaatcc
     1621 cattcagacg ccggatagat tcaagcaagc caacttgtcg tcaaaatcgg tgttgcaaaa
     1681 acgggagtga ccatagattc cgttttctga ggcgaccccc acatagagac cttcccggtc
     1741 attcactttg tagagtttgt ccctgggctt gagattgcgc agcttggtat cggtcagcat
     1801 gtaccttgtc cttcttcgtc tccgataccc actctattgt aaacaagaca tttttatctt
     1861 ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag
     1921 aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg
     1981 ccgctttcct ggctttgctt ccagatgtat gctattctgc tcctgcagct aatggagcac
     2041 cgcaaacagg ttactcgcct ggggattccc tttcgacccg agcatccgta tgagactcat
     2101 gctcgattat tattattata gaagccccca tgaataaatc gctcatcatt ttcggcatcg
     2161 tcaacataac ctcggacagt ttctccgatg gaggccggta tctggcgcca gacgcagcca
     2221 ttgcgcaggc gcgtaagctg atggccgagg gggcagatgt gatcgacctc ggtccggcat
     2281 ccagcaatcc cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg
     2341 tgctggacgc gctcaaggca gatggcattc ccgtctcgct cgacagttat caacccgcga
     2401 cgcaagccta tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc ggttttccag
     2461 acgctgcgtt ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt atgcattcgg
     2521 tgcaagacgg gcaggcagat cggcgcgagg cacccgctgg cgacatcatg gatcacattg
     2581 cggcgttctt tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc
     2641 ttgtccttga tcccggcatg gggttttttc tgggggctgc tcccgaaacc tcgctctcgg
     2701 tgctggcgcg gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt
     2761 cgcgcaaatc ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc ggggccgcga
     2821 cactcgctgc agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc
     2881 cgcgcccctt gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc
     2941 gttaactgca cattcgggat atttctctat attcgcgctt catcagaaaa ctgaaggaac
     3001 ctccattgaa tcgaactaat attttttttg gtgaatcgca ttctgactgg ttgcctgtca
     3061 gaggcggaga atctggtgat tttgtttttc gacgtggtga cgggcatgcc ttcgcgaaaa
     3121 tcgcacctgc ttcccgccgc ggtgagctcg ctggagagcg tgaccgcctc atttggctca
     3181 aaggtcgagg tgtggcttgc cccgaggtca tcaactggca ggaggaacag gagggtgcat
     3241 gcttggtgat aacggcaatt ccgggagtac cggcggctga tctgtctgga gcggatttgc
     3301 tcaaagcgtg gccgtcaatg gggcagcaac ttggcgctgt tcacagccta tcggttgatc
     3361 aatgtccgtt tgagcgcagg ctgtcgcgaa tgttcggacg cgccgttgat gtggtgtccc
     3421 gcaatgccgt caatcccgac ttcttaccgg acgaggacaa gagtacgccg cagctcgatc
     3481 ttttggctcg tgtcgaacga gagctaccgg tgcggctcga ccaagagcgc accgatatgg
     3541 ttgtttgcca tggtgatccc tgcatgccga acttcatggt ggaccctaaa actcttcaat
     3601 gcacgggtct gatcgacctt gggcggctcg gaacagcaga tcgctatgcc gatttggcac
     3661 tcatgattgc taacgccgaa gagaactggg cagcgccaga tgaagcagag cgcgccttcg
     3721 ctgtcctatt caatgtattg gggatcgaag cccccgaccg cgaacgcctt gccttctatc
     3781 tgcgattgga ccctctgact tggggttgat gttcatgccg cctgtttttc ctgctcattg
     3841 gcacgtttcg caacctgttc tcattgcgga caccttttcc agcctcgttt ggaaagtttc
     3901 attgccagac gggactcctg caatcgtcaa gggattgaaa cctatagaag acattgctga
     3961 tgaactgcgc ggggccgact atctggtatg gcgcaatggg aggggagcag tccggttgct
     4021 cggtcgtgag aacaatctga tgttgctcga atatgccggg gagcgaatgc tctctcacat
     4081 cgttgccgag cacggcgact accaggcgac cgaaattgca gcggaactaa tggcgaagct
     4141 gtatgccgca tctgaggaac ccctgccttc tgcccttctc ccgatccggg atcgctttgc
     4201 agctttgttt cagcgggcgc gcgatgatca aaacgcaggt tgtcaaactg actacgtcca
     4261 cgcggcgatt atagccgatc aaatgatgag caatgcctcg gaactgcgtg ggctacatgg
     4321 cgatctgcat catgaaaaca tcatgttctc cagtcgcggc tggctggtga tagatcccgt
     4381 cggtctggtc ggtgaagtgg gctttggcgc cgccaatatg ttctacgatc cggctgacag
     4441 agacgacctt tgtctcgatc ctagacgcat tgcacagatg gcggacgcat tctctcgtgc
     4501 gctggacgtc gatccgcgtc gcctgctcga ccaggcgtac gcttatgggt gcctttccgc
     4561 agcttggaac gcggatggag aagaggagca acgcgatcta gctatcgcgg ccgcgatcaa
     4621 gcaggtgcga cagacgtcat actagatatc aagcgacttc tcctatcccc tgggaacaca
     4681 tcaatctcac cggagaatat cgctggccaa agccttagcg taggattccg ccccttcccg
     4741 caaacgaccc caaacagg
//
LOCUS       DAJVRL010000344         4393 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.199, whole genome shotgun sequence.
ACCESSION   DAJVRL010000344 DAJVRL010000000
VERSION     DAJVRL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.199"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(1..331)
                     /locus_tag="OQN02_004210"
                     /pseudo
     CDS             complement(1..331)
                     /locus_tag="OQN02_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            726..1382
                     /locus_tag="OQN02_004211"
     CDS             726..1382
                     /locus_tag="OQN02_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HCS1644778.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            complement(1701..2117)
                     /locus_tag="OQN02_004212"
     CDS             complement(1701..2117)
                     /locus_tag="OQN02_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1644779.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(2162..3553)
                     /locus_tag="OQN02_004213"
     CDS             complement(2162..3553)
                     /locus_tag="OQN02_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HCS1644780.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(3590..4162)
                     /locus_tag="OQN02_004214"
     CDS             complement(3590..4162)
                     /locus_tag="OQN02_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1644781.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
BASE COUNT         1047 a         1119 c         1051 g         1176 t
ORIGIN      
        1 tccatttttt tgcccggccg aactccacgg cctctttgag tatctcggct tccatcgtct
       61 ttttgcccag aaggcgctgg agttcgcgaa tttgcttatt ggcggcagcg agttcggagg
      121 ccggcaccac ttcctcgccc gatgcaacag ccgtcagtga accgtcttaa tattgcttgc
      181 gccatttgaa tatctggttt gcattgatgc catgcaggcg cgcgacatga gacacggtca
      241 taccgggctc catagtctgc tgaataatgg caattttttc ctgcggtgta cgacgccgac
      301 gccgctcagg tcctgataac acttcaacca tcttatcgtt ctgactggta ttaaacatag
      361 ttccaagact acctcttatt ttaagagagt cgaagtgtct ggtgatctat ggggccagtc
      421 tagccctctt ccaagtgtag ggagatatag tcaaagtttg cttctccgcc gatgagtccg
      481 ccctgcgtcg gatagtccga ttttttaccg gcgaagcacg gctctcctaa ccctatctaa
      541 agcgaataac ttttttcaac agggccgcgt gccaatgcaa gagcgatagg cgctctacgc
      601 gccaatttga ccacttaaaa caggtaaatt gaggggttgt aatgtgttga tgtaacaggc
      661 ttttatttta atgtcttggc atatgtataa tggtagtcta gccctccttt caacaaggag
      721 tactcatgga aacctacaat catacatatc ggcaccacaa cttttcacat aaagacttaa
      781 gtgatctcac cttcaccgct tgcacattca ttcgcagcga ctttcgacgt gctaacttgc
      841 gtgatacgac attcgtcaac tgcaagttca ttgaacaggg tgatatcgaa ggctgccact
      901 ttgatgtcgc agatcttcgt gatgcaagtt tccaacaatg ccaacttgcg atggcaaact
      961 tcagtaatgc caattgctac ggtatagagt tccgtgcgtg tgatttaaaa ggtgccaact
     1021 tttcccgaac aaactttgcc catcaagtga gtaatcgtat gtacttttgc tcagcattta
     1081 tttctggatg taatctttcc tatgccaata tggagagggt ttgtttagaa aaatgtgagt
     1141 tgtttgaaaa tcgctggata ggaacgaacc tagcgggtgc atcactgaaa gagtcagact
     1201 taagtcgagg tgttttttcc gaagatgtct gggggcaatt tagcctacag ggtgccaatt
     1261 tatgccacgc cgaactcgac ggtttagatc cccgcaaagt cgatacatca ggtatcaaaa
     1321 ttgcagcctg gcagcaagaa ctgattctcg aagcactggg tattgttgtt tatcctgact
     1381 aattgctttg atgtgtgatt ttaaacgctc aaatttataa aacgaataat attgtcaacc
     1441 ttgcaataat agacataggg acaaccatgt ataaattttt accctcaggc gtcttcacaa
     1501 tagagccata tagcagtggg tcacttagtg ggcttaattt ttccattaaa gataatatta
     1561 atattgctca gtataaaaca tcatatggta gcccatcttg gcaagtaaac ataaggctgc
     1621 catttataag gcactgttgc aaaaatgtgg aggtgatagg ggtactacgc cggaacccca
     1681 gatttttccg tccgttcaaa ttatccacgg ttgagcagct tgatcccgtc cgcccgcagt
     1741 tgaccaacag gggaaatatg cctgtacaaa gtttgccggg ttatcccaag ttcctggcat
     1801 agcgtactga cctttgtttc tgattgtccc attgatgcca ttgccagtcg cagtttgact
     1861 ggcgtcattt tataaggtcg gccgccgttc cgcccacggg ctcttgctga tgccagacct
     1921 gcagtcgttc tttcagcaat cagttcgcgc tcaaactccg ccagtgcggc aaagataccg
     1981 aagacaagct tgccagcggc cgttgttgtg tcgatagtcg ccccgtgacc ggtcagcact
     2041 ttcagaccgg tccccctcgc ggtcaggtcg tgcactgtat taatgagatg acgaagatca
     2101 cgaccgaggc ggtccagctt ccatacgacc gagagcataa aaaatattgg ggcagcgtga
     2161 gctatgctgc catcgctggt cccttcccgt cactttgaaa acccggatac cagcagacaa
     2221 atttatcctg cagttcgtta ttcaaaacgg cggtccgggt ctgtaacaaa taatgagccc
     2281 cttttctgct ccactgcatc tgctgctttt tggccattcg tctggcgatc acttcattga
     2341 tcgtggattc cacaaacgcg gttgataccg gctctccgta ccgacgcatt tcgccgtagt
     2401 ttgggatcat catcttatta ttccggatgt aggtatacat ttcatccaga tgtttttgca
     2461 gggatttcag gctaggatag ctgagttcag ggtcatcgca atacataacg caattatcaa
     2521 tatgttccag cgcagcgaca acattgccat gccagagata tcgtttaatg ctttccagca
     2581 gtgccagaac tttactcccc gcctctggat ctgataccag cagtccccgg gcatattgca
     2641 tgagcacctt cagcctcatg gtgatatgaa accagtccag cacatgcgtt gactcagggt
     2701 acataccgaa ctggaggtcc ctgagattat ccgcgccgtc ggacagaaaa aatatctgct
     2761 ggttggcctg cattccctgc gctgaaaggt gggtcatgag cctgcgttcc ggatgacagt
     2821 catccttctg aacaaaaccg aagcggcggg tgtcagcagg cgcaccaacg gaaaaagact
     2881 tcccggcaat aatttcaaaa ttacgctttt tgtcatcccg atcgcgaaca taaccaccat
     2941 ctatacccac cacaagcggt tttcctggcc tgggcaggtt cccccagtcg cgggggcagc
     3001 cggaaagaaa acctgaatga gcttcagcct cagcgtcaag acgctgcgcc acctggcata
     3061 aatgattcct caccgttgag gcattcaggc tgtggcccac cggcagaata tctttcagca
     3121 gacgagtcgt catttcatag gagatcatcg aggcccagcg ggtttcaata tatttcagtg
     3181 ccgggtgaga ataatcgccg gcccagtcgc tgagcaaact gactgtcttt gtatcactct
     3241 cttcacaacg gcaccggtac acccgaagcc cggataccgg aataacgcca aacagcgtcc
     3301 ggtaccgtat tttctgtttg cctttgattc tgcgcgcagc aaggcagtga gggcaccgga
     3361 tatggtgttg agtatattcg tctgcctgca gctggaccac cgactgctgg accgtattca
     3421 gcaacagctt ggattctgac accgataatc cgatgtcgtt ccgggtctcc cctgatttct
     3481 ggattgtcat cagttcctcc gttcgactgg agcctgattc atcggtgatg actatctgga
     3541 gcgtcagttg catcattgcc tcctgctgtg gtggatgact atctcccggc tactggttgt
     3601 tcatctggtg acgaagctta taatatgtgg aaacgccaag gcccgcatgg tcgcaggcga
     3661 tcctgagcct gacccctctg cttctgagtg cgtcaatttc ctggatcttt ttgatatcgg
     3721 cgccggggcg ccctgttttt tcaccccgtg cttttttcgc ggcgatgccg tcggcctgcc
     3781 tttccttcag taaggctcgt tcaaactggc tgaatgccga catcatgtgc agctgcagtt
     3841 cctgcatcgg attgtttgtg ccggcgctga acgtcagctg ctctttcaga aaaataaggg
     3901 taacgccctg gtcacgaagc cgggtggtga cagcgcacat atccgacata ttccggcaaa
     3961 ggcgatcgat ggaatgaacc agaagcgtat caccagggcg taattgcccc aggagttcct
     4021 gtaaccccgg gcggtttgtg tccttcgcgc tggcatgctc aacacaaatt ttatcaggct
     4081 gaaaacccgc ctttgacaat gcctcttcct gacgggccgt attctgatcg gccgagctta
     4141 ccctgatgta ggcatattgc acgcaaaacc tccggaaggt ctttaaacat catagagatt
     4201 taaagaataa tccataaaca ctgatttagg gaggtttata gacatttttt cggggatctg
     4261 agaggcatgc tgaaaacgta tacctttata gacatcagtc agcgacgacg gcccagccag
     4321 ataacttcag gctccagtac cggagatgca aggtcgagcc tgctcagttg ccggttgatt
     4381 ttgtggcggt tga
//
LOCUS       DAJVRL010000345         3839 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.205, whole genome shotgun sequence.
ACCESSION   DAJVRL010000345 DAJVRL010000000
VERSION     DAJVRL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.205"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            389..847
                     /gene="gpt"
                     /locus_tag="OQN02_004215"
     CDS             389..847
                     /gene="gpt"
                     /locus_tag="OQN02_004215"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1644782.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            939..2183
                     /gene="frsA"
                     /locus_tag="OQN02_004216"
     CDS             939..2183
                     /gene="frsA"
                     /locus_tag="OQN02_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1644783.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2241..2642
                     /gene="crl"
                     /locus_tag="OQN02_004217"
     CDS             2241..2642
                     /gene="crl"
                     /locus_tag="OQN02_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1644784.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(2681..3736)
                     /gene="phoE"
                     /locus_tag="OQN02_004218"
     CDS             complement(2681..3736)
                     /gene="phoE"
                     /locus_tag="OQN02_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1644785.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT          959 a          926 c          944 g         1010 t
ORIGIN      
        1 tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag gacgggtgag
       61 gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat tgagacagtt
      121 cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt cacagggtta
      181 gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtattgagaa taccacttta
      241 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
      301 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
      361 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
      421 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
      481 cattattgcc gtaagccgtg gcggtctggt accgggggcg ttactggcgc gtgaactggg
      541 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
      601 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
      661 ggataccggt ggtactgctg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
      721 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
      781 agatacctgg atcgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
      841 tcgctaatct tttcaacgcc tggcattgcc gggcgttgtt ctttttaact tcaggcaggt
      901 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
      961 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     1021 atcacggcgc acaaccgcct gtgcagtcgg ctcttgatgg taaaaccatc cctcactggt
     1081 atcgcatgat taaccgtctg atgtggatct ggcgcggtat tgacccacgt gaaatcctcg
     1141 acgtccaggc acgtattgtg atgagcgatg ctgaacgtac cgacgatgat ttatacgata
     1201 ccgtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccacccag gcgatggtgt
     1261 ggcaacaaaa agcctgtacg gaagaagatc cgaaactcag tggtcgacac tggctgcatg
     1321 cggctacgtt gtacaacatt gccgcctatc ctcatctgaa aggagatgac ctggccgagc
     1381 aagcgcaggc tttatcaaac cgcgcctatg aagaggccgc ccagcgtctg ccgggcacga
     1441 tgcggcagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt ttgcatatgc
     1501 cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat gcgatgcaga
     1561 cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgcg atgctgacta
     1621 ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct cacccaggac tccagcctgt
     1681 tgcatcagca cgtcttaaag gcgctgccta acgtaccgtg ggtggatcac actcgcgtcg
     1741 cggcctttgg tttccgtttc ggcgctaacg ttgccgtgcg tctggcatac cttgaatcgc
     1801 cgcgtctgaa agcggttgcc tgtcttggtc cggtagttca taccctgttg agtgatttta
     1861 agtgccagca acaggtgccg gaaatgtatc ttgacgttct ggcgagtcgt ttggggatgc
     1921 atgatgcttc cgacgaagcg ttacgcgtgg agctgaatcg ctattcatta aaagtgcaag
     1981 gattgctggg gcgtcgctgc ccaacgccaa tgttatcagg ctactggaag aacgatccgt
     2041 tcagcccgga agaggactca cgcttaatca cctcatcatc tgctgacggt aaattattag
     2101 agatcccatt taacccggtg tatcggaatt ttgacaaagg tcttcaggaa atcaccggct
     2161 ggatcgaaaa acgcttgtgt taaaaatttg ctaaattttg ccaatttggt aaaacagttg
     2221 catcacaaca ggagatagca atgacgttac cgagtggaca cccgaagagc agattgatca
     2281 aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa gtgcgaagat aatcgattct
     2341 ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc accggaagtg cgtgaattct
     2401 ggggctggtg gatggagctg gaagcgcagg aatcccgttt tacctacagc taccagtttg
     2461 gtctgttcga taaagcaggc gactggaaga gtgttccggt aaaagatact gaagtggttg
     2521 aacgactgga gcacaccctg cgtgagtttc acgagaagct gcgtgaactg ctgacgacgc
     2581 tgaatctgaa gctggaaccg gcgaatgatt ttcgtgatga accggtgaag ttaacggcgt
     2641 gagtgaaatg tgccggatgc atcacatccg gcaatattca ttaaaactga tacgtcatcc
     2701 caaccgcgac agtatcatca ttattaatat tcagcttatt atcgctatcc agttggttga
     2761 ttttataatc aacaaacgct gacatatttt tgttgaaata atacgtagca ccgacgtcga
     2821 tataattgac cagatcttca tcaccgatgc cctcaatatc tttccctttc gataagacat
     2881 aacccagcga tggacgcaga ccaaagtcaa actggtattg agcgaccgct tcaaagttct
     2941 gtgtcttatt ggcaaagccg ccagttattg gcgtcatttt gcgtgtttca gaatagaaag
     3001 ttgccagata aatattattg gcatcgtatt tcagacctgt tgcccatgct tctgcacgct
     3061 tgcctgtgcc acggctttgc aggttctgct cgttggtgcg atctgagttg gtataggccc
     3121 cactaatggc gaaatcgctg ccgccaaagt catatgtcaa tgacgtgccg aagccatcgc
     3181 cgttttgctt tttaacgtcg cggttttcgt ttttcccttg atattgcagg gttaagttca
     3241 ggccatcgat aacgccgaag aagtcggtgt tccgatacgt cgccagaccg ctggcgcgtt
     3301 tggtcataaa gttgtcggtc tgcgccgagg aatcgccacc aaattccggg aacatatcgg
     3361 tccaggcttc cacgtcatac aacgcgccca ggttacgacc ataatcgaaa gaacccaaat
     3421 ctttatattt caacccggca aaagcgagac gcgttttttg ctgtgcagta tcactctccg
     3481 ctttatttcc ggcaaactcc gcttcccacc gaccataacc agtcagttga tcgttaattt
     3541 gagtttcacc tttaaaacca aaacggatat aactctggtc gccatcttta ctggcgttat
     3601 cactcatata atgcatggct ttaactttgc catagacatc cagtttatta ccgtctttat
     3661 tatatatttc tgcagcctgt acagatgcag atgccacaat gcccatcacc actaatgcca
     3721 gagtgctctt tttcattttc attcctgatt ttaattaacg cgcgaatatt cagcgggaga
     3781 gccccgttga aaacaggaaa gtttttaacc tgagattgtt aaagatatat tacagatta
//
LOCUS       DAJVRL010000346         3672 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.207, whole genome shotgun sequence.
ACCESSION   DAJVRL010000346 DAJVRL010000000
VERSION     DAJVRL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.207"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            5..661
                     /locus_tag="OQN02_004219"
     CDS             5..661
                     /locus_tag="OQN02_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QnrS family quinolone resistance pentapeptide
                     repeat protein"
                     /protein_id="HCS1644786.1"
                     /translation="METYNHTYRHHNLSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            complement(980..1396)
                     /locus_tag="OQN02_004220"
     CDS             complement(980..1396)
                     /locus_tag="OQN02_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1644787.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(1441..2832)
                     /locus_tag="OQN02_004221"
     CDS             complement(1441..2832)
                     /locus_tag="OQN02_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HCS1644788.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(2869..3441)
                     /locus_tag="OQN02_004222"
     CDS             complement(2869..3441)
                     /locus_tag="OQN02_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1644789.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
BASE COUNT          887 a          938 c          879 g          968 t
ORIGIN      
        1 actcatggaa acctacaatc atacatatcg gcaccacaac ctttcacata aagacttaag
       61 tgatctcacc ttcaccgctt gcacattcat tcgcagcgac tttcgacgtg ctaacttgcg
      121 tgatacgaca ttcgtcaact gcaagttcat tgaacagggt gatatcgaag gctgccactt
      181 tgatgtcgca gatcttcgtg atgcaagttt ccaacaatgc caacttgcga tggcaaactt
      241 cagtaatgcc aattgctacg gtatagagtt ccgtgcgtgt gatttaaaag gtgccaactt
      301 ttcccgaaca aactttgccc atcaagtgag taatcgtatg tacttttgct cagcatttat
      361 ttctggatgt aatctttcct atgccaatat ggagagggtt tgtttagaaa aatgtgagtt
      421 gtttgaaaat cgctggatag gaacgaacct agcgggtgca tcactgaaag agtcagactt
      481 aagtcgaggt gttttttccg aagatgtctg ggggcaattt agcctacagg gtgccaattt
      541 atgccacgcc gaactcgacg gtttagatcc ccgcaaagtc gatacatcag gtatcaaaat
      601 tgcagcctgg cagcaagaac tgattctcga agcactgggt attgttgttt atcctgacta
      661 attgctttga tgtgtgattt taaacgctca aatttataaa acgaataata ttgtcaacct
      721 tgcaataata gacataggga caaccatgta taaattttta ccctcaggcg tcttcacaat
      781 agagccatat agcagtgggt cacttagtgg gcttaatttt tccattaaag ataatattaa
      841 tattgctcag tataaaacat catatggtag cccatcttgg caagtaaaca taaggctgcc
      901 atttataagg cactgttgca aaaatgtgga ggtgataggg gtactacgcc ggaaccccag
      961 atttttccgt ccgttcaaat tatccacggt tgagcagctt gatcccgtcc gcccgcagtt
     1021 gaccaacagg ggaaatatgc ctgtacaaag tttgccgggt tatcccaagt tcctggcata
     1081 gcgtactgac ctttgtttct gattgtccca ttgatgccat tgccagtcgc agtttgactg
     1141 gcgtcatttt ataaggtcgg ccgccgttcc gcccacgggc tcttgctgat gccagacctg
     1201 cagtcgttct ttcagcaatc agttcgcgct caaactccgc cagtgcggca aagataccga
     1261 agacaagctt gccagcggcc gttgttgtgt cgatagtcgc cccgtgaccg gtcagcactt
     1321 tcagaccggt ccccctcgcg gtcaggtcgt gcactgtatt aatgagatga cgaagatcac
     1381 gaccgaggcg gtccagcttc catacgaccg agagcataaa aaatattggg gcagcgtgag
     1441 ctatgctgcc atcgctggtc ccttcccgtc actttgaaaa cccggatacc agcagacaaa
     1501 tttatcctgc agttcgttat tcaaaacggc ggtccgggtc tgtaacaaat aatgagcccc
     1561 ttttctgctc cactgcatct gctgcttttt ggccattcgt ctggcgatca cttcattgat
     1621 cgtggattcc acaaacgcgg ttgataccgg ctctccgtac cgacgcattt cgccgtagtt
     1681 tgggatcatc atcttattat tccggatgta ggtatacatt tcatccagat gtttttgcag
     1741 ggatttcagg ctaggatagc tgagttcagg gtcatcgcaa tacataacgc aattatcaat
     1801 atgttccagc gcagcgacaa cattgccatg ccagagatat cgtttaatgc tttccagcag
     1861 tgccagaact ttactccccg cctctggatc tgataccagc agtccccggg catattgcat
     1921 gagcaccttc agcctcatgg tgatatgaaa ccagtccagc acatgcgttg actcagggta
     1981 cataccgaac tggaggtccc tgagattatc cgcgccgtcg gacagaaaaa atatctgctg
     2041 gttggcctgc attccctgcg ctgaaaggtg ggtcatgagc ctgcgttccg gatgacagtc
     2101 atccttctga acaaaaccga agcggcgggt gtcagcaggc gcaccaacgg aaaaagactt
     2161 cccggcaata atttcaaaat tacgcttttt gtcatcccga tcgcgaacat aaccaccatc
     2221 tatacccacc acaagcggtt ttcctggcct gggcaggttc ccccagtcgc gggggcagcc
     2281 ggaaagaaaa cctgaatgag cttcagcctc agcgtcaaga cgctgcgcca cctggcataa
     2341 atgattcctc accgttgagg cattcaggct gtggcccacc ggcagaatat ctttcagcag
     2401 acgagtcgtc atttcatagg agatcatcga ggcccagcgg gtttcaatat atttcagtgc
     2461 cgggtgagaa taatcgccgg cccagtcgct gagcaaactg actgtctttg tatcactctc
     2521 ttcacaacgg caccggtaca cccgaagccc ggataccgga ataacgccaa acagcgtccg
     2581 gtaccgtatt ttctgtttgc ctttgattct gcgcgcagca aggcagtgag ggcaccggat
     2641 atggtgttga gtatattcgt ctgcctgcag ctggaccacc gactgctgga ccgtattcag
     2701 caacagcttg gattctgaca ccgataatcc gatgtcgttc cgggtctccc ctgatttctg
     2761 gattgtcatc agttcctccg ttcgactgga gcctgattca tcggtgatga ctatctggag
     2821 cgtcagttgc atcattgcct cctgctgtgg tggatgacta tctcccggct actggttgtt
     2881 catctggtga cgaagcttat aatatgtgga aacgccaagg cccgcatggt cgcaggcgat
     2941 cctgagcctg acccctctgc ttctgagtgc gtcaatttcc tggatctttt tgatatcggc
     3001 gccggggcgc cctgtttttt caccccgtgc ttttttcgcg gcgatgccgt cggcctgcct
     3061 ttccttcagt aaggctcgtt caaactggct gaatgccgac atcatgtgca gctgcagttc
     3121 ctgcatcgga ttgtttgtgc cggcgctgaa cgtcagctgc tctttcagaa aaataagggt
     3181 aacgccctgg tcacgaagcc gggtggtgac agcgcacata tccgacatat tccggcaaag
     3241 gcgatcgatg gaatgaacca gaagcgtatc accagggcgt aattgcccca ggagttcctg
     3301 taaccccggg cggtttgtgt ccttcgcgct ggcatgctca acacaaattt tatcaggctg
     3361 aaaacccgcc tttgacaatg cctcttcctg acgggccgta ttctgatcgg ccgagcttac
     3421 cctgatgtag gcatattgca cgcaaaacct ccggaaggtc tttaaacatc atagagattt
     3481 aaagaataat ccataaacac tgatttaggg aggtttatag acattttttc ggggatctga
     3541 gaggcatgct gaaaacgtat acctttatag acatcagtca gcgacgacgg cccagccaga
     3601 taacttcagg ctccagtacc ggagatgcaa ggtcgagcct gctcagttgc cggttgattt
     3661 tgtggcggtt ga
//
LOCUS       DAJVRL010000347         3652 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.209, whole genome shotgun sequence.
ACCESSION   DAJVRL010000347 DAJVRL010000000
VERSION     DAJVRL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.209"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..641
                     /locus_tag="OQN02_004223"
     CDS             <1..641
                     /locus_tag="OQN02_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="QnrS family quinolone resistance pentapeptide
                     repeat protein"
                     /protein_id="HCS1644790.1"
                     /translation="TYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVNCKFIE
                     QGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNFAHQV
                     SNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGVFSE
                     DVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
     gene            complement(960..1376)
                     /locus_tag="OQN02_004224"
     CDS             complement(960..1376)
                     /locus_tag="OQN02_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1644791.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(1421..2812)
                     /locus_tag="OQN02_004225"
     CDS             complement(1421..2812)
                     /locus_tag="OQN02_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HCS1644792.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(2849..3421)
                     /locus_tag="OQN02_004226"
     CDS             complement(2849..3421)
                     /locus_tag="OQN02_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1644793.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
BASE COUNT          879 a          931 c          877 g          965 t
ORIGIN      
        1 atacatatcg gcaccacaac ttttcacata aagacttaag tgatctcacc ttcaccgctt
       61 gcacattcat tcgcagcgac tttcgacgtg ctaacttgcg tgatacgaca ttcgtcaact
      121 gcaagttcat tgaacagggt gatatcgaag gctgccactt tgatgtcgca gatcttcgtg
      181 atgcaagttt ccaacaatgc caacttgcga tggcaaactt cagtaatgcc aattgctacg
      241 gtatagagtt ccgtgcgtgt gatttaaaag gtgccaactt ttcccgaaca aactttgccc
      301 atcaagtgag taatcgtatg tacttttgct cagcatttat ttctggatgt aatctttcct
      361 atgccaatat ggagagggtt tgtttagaaa aatgtgagtt gtttgaaaat cgctggatag
      421 gaacgaacct agcgggtgca tcactgaaag agtcagactt aagtcgaggt gttttttccg
      481 aagatgtctg ggggcaattt agcctacagg gtgccaattt atgccacgcc gaactcgacg
      541 gtttagatcc ccgcaaagtc gatacatcag gtatcaaaat tgcagcctgg cagcaagaac
      601 tgattctcga agcactgggt attgttgttt atcctgacta attgctttga tgtgtgattt
      661 taaacgctca aatttataaa acgaataata ttgtcaacct tgcaataata gacataggga
      721 caaccatgta taaattttta ccctcaggcg tcttcacaat agagccatat agcagtgggt
      781 cacttagtgg gcttaatttt tccattaaag ataatattaa tattgctcag tataaaacat
      841 catatggtag cccatcttgg caagtaaaca taaggctgcc atttataagg cactgttgca
      901 aaaatgtgga ggtgataggg gtactacgcc ggaaccccag atttttccgt ccgttcaaat
      961 tatccacggt tgagcagctt gatcccgtcc gcccgcagtt gaccaacagg ggaaatatgc
     1021 ctgtacaaag tttgccgggt tatcccaagt tcctggcata gcgtactgac ctttgtttct
     1081 gattgtccca ttgatgccat tgccagtcgc agtttgactg gcgtcatttt ataaggtcgg
     1141 ccgccgttcc gcccacgggc tcttgctgat gccagacctg cagtcgttct ttcagcaatc
     1201 agttcgcgct caaactccgc cagtgcggca aagataccga agacaagctt gccagcggcc
     1261 gttgttgtgt cgatagtcgc cccgtgaccg gtcagcactt tcagaccggt ccccctcgcg
     1321 gtcaggtcgt gcactgtatt aatgagatga cgaagatcac gaccgaggcg gtccagcttc
     1381 catacgaccg agagcataaa aaatattggg gcagcgtgag ctatgctgcc atcgctggtc
     1441 ccttcccgtc actttgaaaa cccggatacc agcagacaaa tttatcctgc agttcgttat
     1501 tcaaaacggc ggtccgggtc tgtaacaaat aatgagcccc ttttctgctc cactgcatct
     1561 gctgcttttt ggccattcgt ctggcgatca cttcattgat cgtggattcc acaaacgcgg
     1621 ttgataccgg ctctccgtac cgacgcattt cgccgtagtt tgggatcatc atcttattat
     1681 tccggatgta ggtatacatt tcatccagat gtttttgcag ggatttcagg ctaggatagc
     1741 tgagttcagg gtcatcgcaa tacataacgc aattatcaat atgttccagc gcagcgacaa
     1801 cattgccatg ccagagatat cgtttaatgc tttccagcag tgccagaact ttactccccg
     1861 cctctggatc tgataccagc agtccccggg catattgcat gagcaccttc agcctcatgg
     1921 tgatatgaaa ccagtccagc acatgcgttg actcagggta cataccgaac tggaggtccc
     1981 tgagattatc cgcgccgtcg gacagaaaaa atatctgctg gttggcctgc attccctgcg
     2041 ctgaaaggtg ggtcatgagc ctgcgttccg gatgacagtc atccttctga acaaaaccga
     2101 agcggcgggt gtcagcaggc gcaccaacgg aaaaagactt cccggcaata atttcaaaat
     2161 tacgcttttt gtcatcccga tcgcgaacat aaccaccatc tatacccacc acaagcggtt
     2221 ttcctggcct gggcaggttc ccccagtcgc gggggcagcc ggaaagaaaa cctgaatgag
     2281 cttcagcctc agcgtcaaga cgctgcgcca cctggcataa atgattcctc accgttgagg
     2341 cattcaggct gtggcccacc ggcagaatat ctttcagcag acgagtcgtc atttcatagg
     2401 agatcatcga ggcccagcgg gtttcaatat atttcagtgc cgggtgagaa taatcgccgg
     2461 cccagtcgct gagcaaactg actgtctttg tatcactctc ttcacaacgg caccggtaca
     2521 cccgaagccc ggataccgga ataacgccaa acagcgtccg gtaccgtatt ttctgtttgc
     2581 ctttgattct gcgcgcagca aggcagtgag ggcaccggat atggtgttga gtatattcgt
     2641 ctgcctgcag ctggaccacc gactgctgga ccgtattcag caacagcttg gattctgaca
     2701 ccgataatcc gatgtcgttc cgggtctccc ctgatttctg gattgtcatc agttcctccg
     2761 ttcgactgga gcctgattca tcggtgatga ctatctggag cgtcagttgc atcattgcct
     2821 cctgctgtgg tggatgacta tctcccggct actggttgtt catctggtga cgaagcttat
     2881 aatatgtgga aacgccaagg cccgcatggt cgcaggcgat cctgagcctg acccctctgc
     2941 ttctgagtgc gtcaatttcc tggatctttt tgatatcggc gccggggcgc cctgtttttt
     3001 caccccgtgc ttttttcgcg gcgatgccgt cggcctgcct ttccttcagt aaggctcgtt
     3061 caaactggct gaatgccgac atcatgtgca gctgcagttc ctgcatcgga ttgtttgtgc
     3121 cggcgctgaa cgtcagctgc tctttcagaa aaataagggt aacgccctgg tcacgaagcc
     3181 gggtggtgac agcgcacata tccgacatat tccggcaaag gcgatcgatg gaatgaacca
     3241 gaagcgtatc accagggcgt aattgcccca ggagttcctg taaccccggg cggtttgtgt
     3301 ccttcgcgct ggcatgctca acacaaattt tatcaggctg aaaacccgcc tttgacaatg
     3361 cctcttcctg acgggccgta ttctgatcgg ccgagcttac cctgatgtag gcatattgca
     3421 cgcaaaacct ccggaaggtc tttaaacatc atagagattt aaagaataat ccataaacac
     3481 tgatttaggg aggtttatag acattttttc ggggatctga gaggcatgct gaaaacgtat
     3541 acctttatag acatcagtca gcgacgacgg cccagccaga taacttcagg ctccagtacc
     3601 ggagatgcaa ggtcgagcct gctcagttgc cggttgattt tgtggcggtt ga
//
LOCUS       DAJVRL010000348         3209 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.213, whole genome shotgun sequence.
ACCESSION   DAJVRL010000348 DAJVRL010000000
VERSION     DAJVRL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.213"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..1296)
                     /locus_tag="OQN02_004227"
     CDS             complement(<1..1296)
                     /locus_tag="OQN02_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HCS1644794.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFF"
     gene            1460..2017
                     /locus_tag="OQN02_004228"
     CDS             1460..2017
                     /locus_tag="OQN02_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1644795.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            2200..3060
                     /locus_tag="OQN02_004229"
     CDS             2200..3060
                     /locus_tag="OQN02_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HCS1644796.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          838 a          755 c          805 g          811 t
ORIGIN      
        1 aaaaaactgc cgccgtgagc tgaactcccg gctgaggtag tcacaggcat tcagagtggt
       61 aaccccggca ggtgcggatg aaaatttaac ggtattcagc agatggggca ggaaacgacg
      121 aacgcgcccg tactgctcca ccatttcttc atgaaaatta tcgtctgagg gccgggcaat
      181 ttcacggaca agcgtgatga tttcagccag cttttgcctt gggatgtagc tgaacacctc
      241 agcacgaatc gattcgtccg gtgtttcttc tttcagcagg tacgaacatg cgctggcgag
      301 cgccaatgca gatttatcca gatccttcag cgagcggagc cgttttttct gcccaatctt
      361 tctggcgtca cggatgataa cggccagcat ggcgtccaga acgtccaatg catcatccag
      421 cgccagcgtt tcccatgcaa ggacaaaggc aaccagaacc gccatccttt tctgcggtga
      481 catcctggca atattgaaca ccgaagtcat accagcataa cgtgcgagat ttttcaggcg
      541 cacagccggg agtgtactca ggttttcagc atgcaggcca aaatcgttca gagttttcca
      601 gcgttcaatt gcttcattaa acgccggacc actgatggtc acagggccct ttttcagtga
      661 ttccagtaaa gacaggcggc tgcaatcagt tggccccagc agcatctcca gctgtgaacg
      721 ctgttcggct gacggtatca gtgccagttt gttccacagg cgcaacgtcg ccttttccct
      781 tacctctgaa atcaaccggg tcagcgtagt ggctccgggg agaataatac gatgttgcat
      841 aagccaccct gtcgccagat cgaaaagcag gccaggacgt tcgttgctta tccagctccg
      901 ggtatataaa agacgggtaa ggcgaaatgt ccagggccag gcaaattcac gatactgata
      961 gtgctgacgt atcagcgctg catgctcacg gcgggtattt tccctctgac cgtattctgc
     1021 aagaacggtg atatcacgaa tcccgagctg tctggcggta aaatgccgga cgccggaagg
     1081 aatatgattc atatcggtga ggaaggtgcc cagaaaacgg acacatccaa tttgcagggc
     1141 aatgcccaga cggttgtgat cacctctgct ttttccgata aattccttgt ctgcttcatc
     1201 aaggtgaaaa tatcgtgcca gctgaagctc atccggttca ccggtgaatc tgccatagct
     1261 ttcagtctgc tcagtggtca gaaagtcaac gggcatatcg gcctccctgc ctgacgggca
     1321 tttagtaaca tttttccaac cgtacgaaat gttataaatt atcggacatc gtaaaactgt
     1381 tacattaata tgtctattaa atcgtaaatt tgtaataata gacatgagtt gtccgatatt
     1441 cgatttaagg tacattttta tgcgactttt tggttacgct cgggtctcaa ccagtcagca
     1501 gtctcttgat cttcaggtca gagcactcaa agacgcaggt gtgaaagcaa accgtatatt
     1561 taccgataag gcatccggca gttcaacaga ccgggaaggg ctggatttgc tgaggatgaa
     1621 ggtggaggaa ggtgatgtca ttctggttaa gaagctcgac cgtcttggcc gcgacactgc
     1681 cgatatgatc caactgataa aggaatttga cgctcagggc gtggcagtcc ggttcattga
     1741 tgacgggatc agtaccgacg gtgatatggg gcaaatggtg gtcaccatcc tgtcggctgt
     1801 ggcacaggct gaacgccgga ggatcctaga acgcacgaat gagggccgac aggaagcaaa
     1861 gctgaaagga atcaaatttg gccgcaggcg taccgtggac aggaacgtcg tgctgacgct
     1921 tcatcagaag ggcactggtg caacggaaat tgctcatcag ctcagtattg cccgctccac
     1981 ggtttataaa attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2041 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2101 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2161 ccctggtaaa tgcttcaata atattgaaaa aggaagagta tgagtattca acattttcgt
     2221 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2281 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2341 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2401 agcactttta aagttctgct atgtggtgcg gtattatccc gtgttgacgc cgggcaagag
     2461 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2521 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2581 agtgataaca ctgctgccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2641 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2701 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     2761 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2821 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2881 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2941 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3001 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3061 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3121 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3181 ttttcgttcc actgagcgtc agaccccta
//
LOCUS       DAJVRL010000349         3015 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.219, whole genome shotgun sequence.
ACCESSION   DAJVRL010000349 DAJVRL010000000
VERSION     DAJVRL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.219"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            <1..279
                     /locus_tag="OQN02_004230"
     CDS             <1..279
                     /locus_tag="OQN02_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1644797.1"
                     /translation="LCSGGQIQCATSHRGKGVAHSLIEFAKKWALSRQLLGIRLETQT
                     NNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            337..1125
                     /gene="aadA1"
                     /locus_tag="OQN02_004231"
     CDS             337..1125
                     /gene="aadA1"
                     /locus_tag="OQN02_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1644798.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1201..1698
                     /locus_tag="OQN02_004232"
     CDS             1201..1698
                     /locus_tag="OQN02_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644799.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1759..2130
                     /locus_tag="OQN02_004233"
     CDS             1759..2130
                     /locus_tag="OQN02_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644800.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2540..2917
                     /locus_tag="OQN02_004234"
     CDS             2540..2917
                     /locus_tag="OQN02_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1644801.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT          788 a          730 c          782 g          715 t
ORIGIN      
        1 ctctgttcag gtggccaaat tcagtgtgcc acttcacacc gaggcaaagg agtcgcgcac
       61 agtctcatcg aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta
      121 gagacacaaa cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc
      181 ggcggcattg acctgttcac gtataaaact agacctcaag tctcgaacga aacagcgatg
      241 tactggtact ggttctcggg agcacaggat gacgcctaac aattcattca agccgacacc
      301 gcttcgcggc gcggcttaat tcaggagtta aacatcatga gggaagcggt gatcgccgaa
      361 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
      421 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
      481 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
      541 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
      601 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
      661 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
      721 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
      781 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
      841 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
      901 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
      961 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctagg
     1021 caggcttatc ttggacaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     1081 gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt
     1141 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagagagct ggggaagact
     1201 atgcgcgatc tgttgaaggt ggttctaagc ctcgtacttg cgatggcatc ggggcaggca
     1261 cttgctgacc tgccaattgt tttagtggat gaagctcgtc ttccctatga ctactcccca
     1321 tccaactacg acatttctcc aagcaactac gacaactcca taagcaatta cgacaatagt
     1381 ccatcaaatt acgacaactc tgagagcaac tacgataata gttcatccaa ttacgacaat
     1441 agtcgcaacg gaaatcgtag gcttatatat agcgcaaatg ggtctcgcac tttcgccggc
     1501 tactacgtca ttgccaacaa tgggacaacg aacttctttt ccacatctgg caaaaggatg
     1561 ttctacaccc caaaaggggg gcgcggcgtc tatggcggca aagatgggag cttctgcggg
     1621 gcattggtcg tcataaatgg ccaattttcg cttgccctga cagataacgg cctgaagatc
     1681 atgtatctaa gcaactagcc tgctctctaa taaaatgtta ggcctcaaca tctagtcgca
     1741 agctgagggg aaccactagt gtcatacgaa cctccaagag acggttacac aaacgggtac
     1801 attgttgatg tcatgtatga caatcgccca agtaagtatc cagctgtgtt cagaacgtac
     1861 gtccgaattc cagactatgt cgaactagat aacgcggagg agctggccga cgcaatcatt
     1921 cgagaggttg agttaaagga ggcatggctc acaagggtct tggggaactc agccgatcct
     1981 gacgcttctg tgggcaagtg ggaccggcgc ctagcgcagt gcggtggtat tgatgagatg
     2041 tcaagtgggg tgcaggcaac atacttcagt ccaaacggtc agctggtcgg cttagtggca
     2101 tacattgatg ccgaatggat ggatgaatag ctctgacgtt acgattcagg tgagacaacc
     2161 agatgggcat tgcgtacaag ctcagttgcc accgatgtgg ctacgagtct gatcttcttt
     2221 acctaggcca aggtatggcg atgcttcctg agcaaatagt cggaacgtgc acatgctgcg
     2281 acaatctaac gacgatttct ataaatgaaa gctacggagt atgctcactt tgtggggcta
     2341 aggatcgtgt gatatttaca agctatcagc atgaggcaag gccaagaaat ccctgctatc
     2401 tagagagaag tttcaaatat caatgcccaa gatgccatgc cttcagtatg gagcctccgg
     2461 atctaccgga gatgctttgg gactaatgct ccgctttgcc tcgggtatcc gttcgaccta
     2521 tgtttaagag gaaacgcacg tgtctgccgc caaagctctt gcctatgcac tttgtgacaa
     2581 gttggaaagc aaaaaagttg atgaagtcgt acgaaatctt gtttctagcg gctggaatat
     2641 tagggaagtg gcctgggaag cttcgtcctt tgatcatgaa atgccctact ttgacacggg
     2701 gcgggacttc gatattggat ttcctgacac gctgtcatcc aagattgagt ttttgaccgc
     2761 gaatggatca atcgtccaag cacgtgctcg cctgcacttc aagcaggctc taatttttgc
     2821 caaggcgtcc aaacatgtcg ccgcgctcaa gaactcattg gattactact atggatcgtc
     2881 tgtaatcccc cctcaaaacc acggaggcta tttgtagaaa attccattaa tatgaactca
     2941 aggtgttccc tacggacgaa tcagcgatga aggtggtgta cctggctatc caggcggcga
     3001 ccaagaaatg gacga
//
LOCUS       DAJVRL010000350         2570 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.223, whole genome shotgun sequence.
ACCESSION   DAJVRL010000350 DAJVRL010000000
VERSION     DAJVRL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.223"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(40..312)
                     /locus_tag="OQN02_004235"
                     /pseudo
     CDS             complement(40..312)
                     /locus_tag="OQN02_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(705..1718)
                     /gene="intI1"
                     /locus_tag="OQN02_004236"
     CDS             complement(705..1718)
                     /gene="intI1"
                     /locus_tag="OQN02_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1644802.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1874..2347
                     /locus_tag="OQN02_004237"
     CDS             1874..2347
                     /locus_tag="OQN02_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCS1644803.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          611 a          667 c          694 g          598 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgcca gttattccgc gtttcttcag
       61 gtcgtcgagt aaagtaatga cttgacccaa tgaaccgccg agccgatcta gtgcccaaac
      121 tactagggta tctccctcgc gcaatgcttt caggcagttc tccagttccg gcgcaccttt
      181 tttgtcgcgc tttgggccgc tacgtgaggt ctgatcctga tagatttgct cacatccagc
      241 ttttgttagt tcgtcaacct ggtgcgccac atcctgaaga tgcgtagatt tacgtgcata
      301 gccgattttc attcttttct cgctaattag ttatggggtt attgttatgt tgatacagta
      361 acgagttttg ttacatgagg ggagtcattt ttcgggagaa gtcaggactt ttcaagactg
      421 tcacaaaaac catcgttttt gatacattaa tttaaccaat aggttgcaga tcaaatcgcc
      481 tgtaacagcc tttctggctg tttgtcgttt tcagaagacg gctgcactga acgtcagaag
      541 ccgactgcac tatagcagcg gaggggttgg atccatcagg caacgacggg ctgctgccgg
      601 ccatcagcgg acgcagggag gactttccgc aaccggccgt tcgatgcggc accgatggcc
      661 ttcgcgcagg ggtagtgaat ccgccaggat tgacttgcgc tgccctacct ctcactagtg
      721 aggggcggca gcgcatcaag cggtgagcgc actccggcac cgccaacttt cagcacatgc
      781 gtgtaaatca tcgtcgtaga gacgtcggaa tggccgagca gatcctgcac ggttcgaatg
      841 tcgtaaccgc tgcggagcaa ggccgtcgcg aacgagtggc ggagggtgtg cggtgtggcg
      901 ggcttcgtga tgcctgcttg ttctacggca cgtttgaagg cgcgctgaaa ggtctggtca
      961 tacatgtgat ggcgacgcac gacaccgctc cgtggatcgg tcgaatgcgt gtgctgcgca
     1021 aaaacccaga accacggcca ggaatgcccg gcgcgcggat acttccgctc aagggcgtcg
     1081 ggaagcgcaa cgccgctgcg gccctcggcc tggtccttca gccaccatgc ccgtgcacgc
     1141 gacagctgct cgcgcaggct gggtgccaag ctctcgggta acatcaaggc ccgatccttg
     1201 gagcccttgc cctcccgcac gatgatcgtg ccgtgatcga aatccagatc cttgacccgc
     1261 agttgcaaac cctcactgat ccgcatgccc gttccataca gaagctgggc gaacaaacga
     1321 tgctcgcctt ccagaaaacc gaggatgcga accacttcat ccggggtcag caccaccggc
     1381 aagcgccgcg acggccgagg tcttccgatc tcctgaagcc agggcagatc cgtgcacagc
     1441 accttgccgt agaagaacag caaggccgcc aatgcctgac gatgcgtgga gaccgaaacc
     1501 ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc
     1561 gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg
     1621 gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     1681 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     1741 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     1801 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaacc
     1861 caggatgaga accttgaaag tatcattgat ggctgcgaaa gcgaaaaacg gcgtgattgg
     1921 ttgcggtcca gacataccct ggtccgcgaa aggggagcag ctacttttta aagcattgac
     1981 ctacaatcag tggcttctgg tgggtcgcaa gacgtttgaa tctatgggcg cactccccaa
     2041 taggaaatac gcggtcgtta cccgctcagg ttggacatca aatgatgaca atgtagttgt
     2101 atttcagtca atcgaagagg ccatggacag gctagctgaa ttcaccggtc acgttatagt
     2161 gtctggtggc ggagaaattt accgagaaac attacccatg gcctctacgc tccacttatc
     2221 gacgatcgac atcgagccag agggggatgt tttcttcccg agtattccaa ataccttcga
     2281 agttgttttt gagcaacact ttacttcaaa cattaactat tgctatcaaa tttggaaaaa
     2341 gggttaacaa agctatgcaa tcgacggcaa aaagcttcgt tcgcttcgcg cactacgcct
     2401 tttccgcgat tgatagcgac gttatgtgaa tattgaaatg agcattccaa agaaaggaag
     2461 tagaaaaatt atcgtgggcg aaaatgagtt tttgtggctc attaggtcaa aactcacata
     2521 ttcgcaggac ggcactgttg caaatagtcg gtggtgataa acttatcatc
//
LOCUS       DAJVRL010000351         1745 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504032
            SAMEA8900204-rid15857443.guided.251, whole genome shotgun sequence.
ACCESSION   DAJVRL010000351 DAJVRL010000000
VERSION     DAJVRL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900204
            Sequence Read Archive: ERR6005077
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477991.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477991.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:49:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 3,846
            CDSs (with protein)               :: 3,846
            Genes (RNA)                       :: 68
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 58
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 151 of 326
            Pseudo Genes (incomplete)         :: 62 of 326
            Pseudo Genes (internal stop)      :: 139 of 326
            Pseudo Genes (multiple problems)  :: 25 of 326
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900204-rid15857443.guided.251"
                     /serovar="S. flexneri 1b"
                     /isolate="504032"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-12-22"
     gene            complement(<1..524)
                     /gene="intI2"
                     /locus_tag="OQN02_004238"
     CDS             complement(<1..524)
                     /gene="intI2"
                     /locus_tag="OQN02_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCS1644804.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIP"
     gene            856..1329
                     /locus_tag="OQN02_004239"
     CDS             856..1329
                     /locus_tag="OQN02_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1644805.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1424..1708
                     /locus_tag="OQN02_004240"
                     /pseudo
     CDS             1424..1708
                     /locus_tag="OQN02_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat1"
BASE COUNT          518 a          356 c          382 g          489 t
ORIGIN      
        1 gggattaggc gcgtgggcag taggctgttt ctgcttttcc cacccttacc gtcatgcaca
       61 gtgatgcagc cattatcaaa atcaaaatct ttaacccgca aacgcaagca ttcattaatg
      121 cgcaaacctg caccatacag cagcgtaaaa ataacttggt tgcgagtatc cataacctgc
      181 aaaatgcgtt gcacttcatt tgcagagata acagagggta gccgtctagg cttgcttgca
      241 gggatataat caatatcgcc caacggctgt tgtaaaaacc tgttgtacaa aaaagctagg
      301 gcatttaaag cgattttctg cgtgtttatg gctacatgtc tgctgtttgc taagctggat
      361 aaaaacagcc tgacctcttc actgcccatg gtctgaggat gacgtttttt gtgaaacaga
      421 ataaaacgct taatccagtg caggtaagtt ttttcagttt tcagcgcata acctttttgt
      481 cgcatatccg tgcgtataga atttaaaaat ggactgttag acataaaacg ctccttgtct
      541 tgcaactgtc tgcctataca gcctattcta gctgggattt aaaaaagtgc ctgtttttta
      601 cgcctagaga tgcttgttta ccggtagagt tttaatttaa tgctaaataa attaaaatgt
      661 tatgagttct ttgggtgaga taatgtgcat cgtgcaagca ggatagacgg catgcacgat
      721 ttgtaataac agagtgtctt gtatttttaa agaaagtcta tttaatacaa gtgattatat
      781 taattaacgg taagcatcag cgggtgacaa aacgagcatg cttactaata aaatgttaac
      841 ctctgaggaa gaattgtgaa actatcacta atggtagcta tatcgaagaa tggagttatc
      901 gggaatggcc ctgatattcc atggagtgcc aaaggtgaac agctcctgtt taaagctatt
      961 acctataacc aatggctgtt ggttggacgc aagacttttg aatcaatggg agcattaccc
     1021 aaccgaaagt atgcggtcgt aacacgttca agttttacat ctgacaatga gaacgtattg
     1081 atctttccat caattaaaga tgctttaacc aacctaaaga aaataacgga tcatgtcatt
     1141 gtttcaggtg gtggggagat atacaaaagc ctgatcgatc aagtagatac actacatata
     1201 tctacaatag acatcgagcc ggaaggtgat gtttactttc ctgaaatccc cagcaatttt
     1261 aggccagttt ttacccaaga cttcgcctct aacataaatt atagttacca aatctggcaa
     1321 aagggttaac aagtggcagc aacggattcg caaacctgtc acgccttttg taccaaaagc
     1381 cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt catatgaaga tttcggtgat
     1441 ccctgagcag gtggcggaaa cattggatgc tgagaaccat ttcattgttc gtgaagtgtt
     1501 cgatgtgcac ctatccgacc aaggctttga actatctacc agaagtgtga gcccctaccg
     1561 gaaggattac atctcggatg atgactctga tgaagactct gcttgctatg gcgcattcat
     1621 cgaccaagag cttgtcggga agattgaact caactcaaca tggaacgatc tagcctctat
     1681 cgaacacatt gttgtgtcgc acacgcactg aagtggtcaa caaaaactgg ccaccgagtt
     1741 agagt
//
